LOCUS       DACEUF010000001       125573 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.001, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000001 DACEUF010000000
VERSION     DACEUF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 125573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 125573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..125573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.001"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(177..2363)
                     /locus_tag="IXG20_000001"
     CDS             complement(177..2363)
                     /locus_tag="IXG20_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1608228.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2581..3264
                     /locus_tag="IXG20_000002"
     CDS             2581..3264
                     /locus_tag="IXG20_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ1608229.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3345..4064)
                     /locus_tag="IXG20_000003"
     CDS             complement(3345..4064)
                     /locus_tag="IXG20_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1608230.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4376..4582)
                     /locus_tag="IXG20_000004"
     CDS             complement(4376..4582)
                     /locus_tag="IXG20_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608231.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4827..5429
                     /locus_tag="IXG20_000005"
     CDS             4827..5429
                     /locus_tag="IXG20_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ1608232.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5570..6851
                     /locus_tag="IXG20_000006"
                     /pseudo
     CDS             5570..6851
                     /locus_tag="IXG20_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            6864..7592
                     /locus_tag="IXG20_000007"
     CDS             6864..7592
                     /locus_tag="IXG20_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1608233.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7661..9184)
                     /locus_tag="IXG20_000008"
     CDS             complement(7661..9184)
                     /locus_tag="IXG20_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1608234.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9436..10617
                     /locus_tag="IXG20_000009"
     CDS             9436..10617
                     /locus_tag="IXG20_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1608235.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10677..12305)
                     /gene="treC"
                     /locus_tag="IXG20_000010"
     CDS             complement(10677..12305)
                     /gene="treC"
                     /locus_tag="IXG20_000010"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ1608236.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12756..15467)
                     /locus_tag="IXG20_000011"
     CDS             complement(12756..15467)
                     /locus_tag="IXG20_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1608237.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15477..16178)
                     /locus_tag="IXG20_000012"
     CDS             complement(15477..16178)
                     /locus_tag="IXG20_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1608238.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16364..16963
                     /locus_tag="IXG20_000013"
     CDS             16364..16963
                     /locus_tag="IXG20_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1608239.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            17044..18057
                     /locus_tag="IXG20_000014"
     CDS             17044..18057
                     /locus_tag="IXG20_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1608240.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18165..19082
                     /gene="rbsK"
                     /locus_tag="IXG20_000015"
     CDS             18165..19082
                     /gene="rbsK"
                     /locus_tag="IXG20_000015"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ1608241.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19110..19505
                     /gene="rbsD"
                     /locus_tag="IXG20_000016"
     CDS             19110..19505
                     /gene="rbsD"
                     /locus_tag="IXG20_000016"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ1608242.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19505..20878
                     /locus_tag="IXG20_000017"
     CDS             19505..20878
                     /locus_tag="IXG20_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1608243.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21235..23379
                     /locus_tag="IXG20_000018"
     CDS             21235..23379
                     /locus_tag="IXG20_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1608244.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23431..24472)
                     /locus_tag="IXG20_000019"
                     /pseudo
     CDS             complement(23431..24472)
                     /locus_tag="IXG20_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(24749..26551)
                     /locus_tag="IXG20_000020"
     CDS             complement(24749..26551)
                     /locus_tag="IXG20_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1608245.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26565..27317)
                     /locus_tag="IXG20_000021"
     CDS             complement(26565..27317)
                     /locus_tag="IXG20_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1608246.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27326..27688)
                     /locus_tag="IXG20_000022"
     CDS             complement(27326..27688)
                     /locus_tag="IXG20_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1608247.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27864..28736)
                     /locus_tag="IXG20_000023"
     CDS             complement(27864..28736)
                     /locus_tag="IXG20_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ1608248.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28787..29275)
                     /locus_tag="IXG20_000024"
     CDS             complement(28787..29275)
                     /locus_tag="IXG20_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1608249.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29413..30204)
                     /locus_tag="IXG20_000025"
     CDS             complement(29413..30204)
                     /locus_tag="IXG20_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ1608250.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GKSYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(30259..30867)
                     /locus_tag="IXG20_000026"
     CDS             complement(30259..30867)
                     /locus_tag="IXG20_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ1608251.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30860..31177)
                     /locus_tag="IXG20_000027"
     CDS             complement(30860..31177)
                     /locus_tag="IXG20_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1608252.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31333..32061)
                     /locus_tag="IXG20_000028"
     CDS             complement(31333..32061)
                     /locus_tag="IXG20_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ1608253.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32220..33101
                     /locus_tag="IXG20_000029"
     CDS             32220..33101
                     /locus_tag="IXG20_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1608254.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERANRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33338..34381)
                     /locus_tag="IXG20_000030"
     CDS             complement(33338..34381)
                     /locus_tag="IXG20_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1608255.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34384..35052)
                     /locus_tag="IXG20_000031"
     CDS             complement(34384..35052)
                     /locus_tag="IXG20_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1608256.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35194..37530
                     /locus_tag="IXG20_000032"
     CDS             35194..37530
                     /locus_tag="IXG20_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1608257.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37645..37956)
                     /locus_tag="IXG20_000033"
     CDS             complement(37645..37956)
                     /locus_tag="IXG20_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608258.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            38137..39612
                     /locus_tag="IXG20_000034"
     CDS             38137..39612
                     /locus_tag="IXG20_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ1608259.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39659..40243
                     /locus_tag="IXG20_000035"
     CDS             39659..40243
                     /locus_tag="IXG20_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ1608260.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            40245..40664
                     /locus_tag="IXG20_000036"
     CDS             40245..40664
                     /locus_tag="IXG20_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ1608261.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(40945..41229)
                     /locus_tag="IXG20_000037"
     CDS             complement(40945..41229)
                     /locus_tag="IXG20_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ1608262.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41461..42171)
                     /locus_tag="IXG20_000038"
     CDS             complement(41461..42171)
                     /locus_tag="IXG20_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ1608263.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42168..43640)
                     /locus_tag="IXG20_000039"
     CDS             complement(42168..43640)
                     /locus_tag="IXG20_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1608264.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            43778..44251
                     /locus_tag="IXG20_000040"
     CDS             43778..44251
                     /locus_tag="IXG20_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1608265.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44404..44955)
                     /locus_tag="IXG20_000041"
     CDS             complement(44404..44955)
                     /locus_tag="IXG20_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1608266.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45060..45590
                     /locus_tag="IXG20_000042"
     CDS             45060..45590
                     /locus_tag="IXG20_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ1608267.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46073..46516)
                     /locus_tag="IXG20_000043"
     CDS             complement(46073..46516)
                     /locus_tag="IXG20_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1608268.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46532..47692)
                     /locus_tag="IXG20_000044"
     CDS             complement(46532..47692)
                     /locus_tag="IXG20_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1608269.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48199..49899
                     /locus_tag="IXG20_000045"
     CDS             48199..49899
                     /locus_tag="IXG20_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608270.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(50001..50834)
                     /locus_tag="IXG20_000046"
     CDS             complement(50001..50834)
                     /locus_tag="IXG20_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ1608271.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(51138..53303)
                     /locus_tag="IXG20_000047"
     CDS             complement(51138..53303)
                     /locus_tag="IXG20_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1608272.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            53563..55857
                     /locus_tag="IXG20_000048"
     CDS             53563..55857
                     /locus_tag="IXG20_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1608273.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            55850..56515
                     /gene="pgmB"
                     /locus_tag="IXG20_000049"
     CDS             55850..56515
                     /gene="pgmB"
                     /locus_tag="IXG20_000049"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1608274.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            56508..57515
                     /locus_tag="IXG20_000050"
     CDS             56508..57515
                     /locus_tag="IXG20_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1608275.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            57537..58556
                     /locus_tag="IXG20_000051"
     CDS             57537..58556
                     /locus_tag="IXG20_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1608276.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(58837..60204)
                     /gene="nhaC"
                     /locus_tag="IXG20_000052"
     CDS             complement(58837..60204)
                     /gene="nhaC"
                     /locus_tag="IXG20_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1608277.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(60313..61734)
                     /locus_tag="IXG20_000053"
     CDS             complement(60313..61734)
                     /locus_tag="IXG20_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1608278.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(61977..63854)
                     /gene="tdc"
                     /locus_tag="IXG20_000054"
     CDS             complement(61977..63854)
                     /gene="tdc"
                     /locus_tag="IXG20_000054"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1608279.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(64145..65401)
                     /locus_tag="IXG20_000055"
     CDS             complement(64145..65401)
                     /locus_tag="IXG20_000055"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ1608280.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            65981..67282
                     /locus_tag="IXG20_000056"
     CDS             65981..67282
                     /locus_tag="IXG20_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1608281.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(67351..67950)
                     /locus_tag="IXG20_000057"
     CDS             complement(67351..67950)
                     /locus_tag="IXG20_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1608282.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            68204..69085
                     /locus_tag="IXG20_000058"
     CDS             68204..69085
                     /locus_tag="IXG20_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1608283.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            69098..70707
                     /locus_tag="IXG20_000059"
                     /pseudo
     CDS             69098..70707
                     /locus_tag="IXG20_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
     gene            complement(70784..71443)
                     /locus_tag="IXG20_000060"
     CDS             complement(70784..71443)
                     /locus_tag="IXG20_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1608284.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(71726..72391)
                     /locus_tag="IXG20_000061"
     CDS             complement(71726..72391)
                     /locus_tag="IXG20_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608285.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(72538..73143)
                     /gene="dhaL"
                     /locus_tag="IXG20_000062"
     CDS             complement(72538..73143)
                     /gene="dhaL"
                     /locus_tag="IXG20_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ1608286.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(73171..74154)
                     /gene="dhaK"
                     /locus_tag="IXG20_000063"
     CDS             complement(73171..74154)
                     /gene="dhaK"
                     /locus_tag="IXG20_000063"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ1608287.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(74156..74551)
                     /gene="dhaM"
                     /locus_tag="IXG20_000064"
     CDS             complement(74156..74551)
                     /gene="dhaM"
                     /locus_tag="IXG20_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ1608288.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            74705..75025
                     /locus_tag="IXG20_000065"
     CDS             74705..75025
                     /locus_tag="IXG20_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ1608289.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(75137..76006)
                     /locus_tag="IXG20_000066"
     CDS             complement(75137..76006)
                     /locus_tag="IXG20_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1608290.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(76102..77406)
                     /locus_tag="IXG20_000067"
     CDS             complement(76102..77406)
                     /locus_tag="IXG20_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1608291.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFI
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(77403..77912)
                     /locus_tag="IXG20_000068"
     CDS             complement(77403..77912)
                     /locus_tag="IXG20_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608292.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(78087..80222)
                     /locus_tag="IXG20_000069"
     CDS             complement(78087..80222)
                     /locus_tag="IXG20_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1608293.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(80305..81960)
                     /locus_tag="IXG20_000070"
     CDS             complement(80305..81960)
                     /locus_tag="IXG20_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1608294.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKKLDGAFSLYQTVRPEELIYPNK"
     gene            complement(81962..82276)
                     /locus_tag="IXG20_000071"
     CDS             complement(81962..82276)
                     /locus_tag="IXG20_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1608295.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            82446..82706
                     /locus_tag="IXG20_000072"
     CDS             82446..82706
                     /locus_tag="IXG20_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ1608296.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            82720..83034
                     /locus_tag="IXG20_000073"
     CDS             82720..83034
                     /locus_tag="IXG20_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ1608297.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(83519..84073)
                     /locus_tag="IXG20_000074"
     CDS             complement(83519..84073)
                     /locus_tag="IXG20_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ1608298.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(84199..86129)
                     /locus_tag="IXG20_000075"
                     /pseudo
     CDS             complement(84199..86129)
                     /locus_tag="IXG20_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(86435..87151)
                     /locus_tag="IXG20_000076"
     CDS             complement(86435..87151)
                     /locus_tag="IXG20_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1608299.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(87152..88975)
                     /gene="glpO"
                     /locus_tag="IXG20_000077"
     CDS             complement(87152..88975)
                     /gene="glpO"
                     /locus_tag="IXG20_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ1608300.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(88989..90485)
                     /gene="glpK"
                     /locus_tag="IXG20_000078"
     CDS             complement(88989..90485)
                     /gene="glpK"
                     /locus_tag="IXG20_000078"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ1608301.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(90621..92081)
                     /locus_tag="IXG20_000079"
     CDS             complement(90621..92081)
                     /locus_tag="IXG20_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1608302.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(92071..93366)
                     /locus_tag="IXG20_000080"
     CDS             complement(92071..93366)
                     /locus_tag="IXG20_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1608303.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(93698..94375)
                     /locus_tag="IXG20_000081"
     CDS             complement(93698..94375)
                     /locus_tag="IXG20_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ1608304.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(94405..95496)
                     /locus_tag="IXG20_000082"
     CDS             complement(94405..95496)
                     /locus_tag="IXG20_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ1608305.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(95597..96031)
                     /locus_tag="IXG20_000083"
     CDS             complement(95597..96031)
                     /locus_tag="IXG20_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1608306.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(96615..98072)
                     /gene="ascB"
                     /locus_tag="IXG20_000084"
     CDS             complement(96615..98072)
                     /gene="ascB"
                     /locus_tag="IXG20_000084"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1608307.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(98069..99964)
                     /locus_tag="IXG20_000085"
     CDS             complement(98069..99964)
                     /locus_tag="IXG20_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1608308.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(100022..101797)
                     /locus_tag="IXG20_000086"
     CDS             complement(100022..101797)
                     /locus_tag="IXG20_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1608309.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(101847..103343)
                     /locus_tag="IXG20_000087"
     CDS             complement(101847..103343)
                     /locus_tag="IXG20_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1608310.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(103537..105159)
                     /locus_tag="IXG20_000088"
     CDS             complement(103537..105159)
                     /locus_tag="IXG20_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1608311.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(105487..106800)
                     /locus_tag="IXG20_000089"
     CDS             complement(105487..106800)
                     /locus_tag="IXG20_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1608312.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(107045..107269)
                     /locus_tag="IXG20_000090"
     CDS             complement(107045..107269)
                     /locus_tag="IXG20_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608313.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(107289..107657)
                     /locus_tag="IXG20_000091"
     CDS             complement(107289..107657)
                     /locus_tag="IXG20_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1608314.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(107853..108563)
                     /locus_tag="IXG20_000092"
     CDS             complement(107853..108563)
                     /locus_tag="IXG20_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608315.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(108581..109078)
                     /locus_tag="IXG20_000093"
     CDS             complement(108581..109078)
                     /locus_tag="IXG20_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1608316.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(109115..109567)
                     /locus_tag="IXG20_000094"
     CDS             complement(109115..109567)
                     /locus_tag="IXG20_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1608317.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(109702..110307)
                     /locus_tag="IXG20_000095"
     CDS             complement(109702..110307)
                     /locus_tag="IXG20_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1608318.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETSEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(110757..111377)
                     /locus_tag="IXG20_000096"
     CDS             complement(110757..111377)
                     /locus_tag="IXG20_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1608319.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(111379..112248)
                     /locus_tag="IXG20_000097"
     CDS             complement(111379..112248)
                     /locus_tag="IXG20_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ1608320.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(112271..112678)
                     /locus_tag="IXG20_000098"
     CDS             complement(112271..112678)
                     /locus_tag="IXG20_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1608321.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(112697..113986)
                     /locus_tag="IXG20_000099"
     CDS             complement(112697..113986)
                     /locus_tag="IXG20_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1608322.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(114013..114315)
                     /locus_tag="IXG20_000100"
     CDS             complement(114013..114315)
                     /locus_tag="IXG20_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1608323.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(114340..116433)
                     /locus_tag="IXG20_000101"
     CDS             complement(114340..116433)
                     /locus_tag="IXG20_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1608324.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(116922..117650)
                     /locus_tag="IXG20_000102"
     CDS             complement(116922..117650)
                     /locus_tag="IXG20_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ1608325.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(117796..120417)
                     /locus_tag="IXG20_000103"
                     /pseudo
     CDS             complement(117796..120417)
                     /locus_tag="IXG20_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(120531..121862)
                     /locus_tag="IXG20_000104"
     CDS             complement(120531..121862)
                     /locus_tag="IXG20_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1608326.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(121828..123306)
                     /locus_tag="IXG20_000105"
     CDS             complement(121828..123306)
                     /locus_tag="IXG20_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1608327.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(123611..125446)
                     /gene="tdc"
                     /locus_tag="IXG20_000106"
     CDS             complement(123611..125446)
                     /gene="tdc"
                     /locus_tag="IXG20_000106"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1608328.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
BASE COUNT        36692 a        26110 c        21910 g        40861 t
ORIGIN      
        1 ataaataagg gaggtgtgac aaaattcttg tcacacctcc cttttccgaa taaactgtgt
       61 tccagaaata gcttcttcga aaataagctg aaatttcaca aaaatttgag aagcaatttt
      121 cgtacattcc ttcttctttc ttgaaactga ccacttctgt cacaccctct cttctatcac
      181 tttccttttt tcacacgact cccggtttct ttgataaatc tcgatacttt caattttctt
      241 gaatttttca tttgaggtac acatagatac agacgatttc ttgctctagt catcgcaaca
      301 taaaatagtc ttcgttcttc ttctatcgtc gttactggtt ctgcttttat ttcattaaag
      361 tcgattactt tcttttctgt cgctccgtta taaggcaaac ttccttcgtt acaaccaatc
      421 aaaaagacat ttcggtaact aagaccttta cttttgtgaa tgctctggat acatagtgca
      481 tcaagttttc cttgtctgta agcaatcaag cgggttttta attctttagc tgtcgcttgg
      541 gtttcttgcg ataaggcttg gagtcttctg aaagtagcat gctgctttac ttcttctcgt
      601 atccattctt gatcactctg gtcgatttcc ttagctatac gtggcagatg aagatagctt
      661 tgaacaagcg aatagatttc ttgatgctgg tctgttttac gcaacgcttc ctcaaactct
      721 cggatttctg cagatttcga ttttaaattg gggcttatcg acaaaatata atctgcaacg
      781 gatatatttt gatatttagg agaaaagtat tgatcgcatt cacttttttt caggtagcag
      841 tctgccatca gctgccggag caattgctgg taggcctgat ttttcttttg cagattcagt
      901 gctgtttgcc aatgaacaat tttttgatag aaatgttgga aagacttgat accaaagata
      961 ccttcatatg gactatctgc ttccagtaca aaaggaatat tcgttttcag cagagattgg
     1021 acaagctgct gcgtttgtga agctgatcgg tataatatga ttgattcctt atatttccca
     1081 tttttttgaa taaatgtctg aatttgtttg attagccact gactttctgc tttagtatca
     1141 gaaaacaaat aggcttccat aacacctgct tctttttgtg ctggaatcat cgcctttggt
     1201 atccgatcat ttttattata gctgatcaat ctatttgctc cttggacaat agctgcccga
     1261 gaacgataat tagttaataa gaaaagagtt ttggtaggat attctttttc aaagttcttg
     1321 atgtatgctg gctctgatcc tctgaatcca tagatactct gatcatcatc tccaacaaca
     1381 atcaacttga tcctgttttc ctttgtcatc cgtttcacga tttcccattg aagtggatta
     1441 gtatcttgaa attcatcaat gaatagataa tcatattttt ggccgatata atgctggatc
     1501 tcctcattct ccaaagcgct tttcagattt tggagtacat cattgaactc tagcaattgc
     1561 tggcttcttt tttgttcttg gagttccgtg agcaatccat acagtcgttc tgatatcggc
     1621 tgtcgtgcat tcttatcaat ggaatcatat ttttctttaa ctaactgaac catttcttct
     1681 actgaaaggt tcatattctg ccaatagtct aaccgagaag caatcgtttt ttctccatat
     1741 tgatcaaata tataaagatt atatttacgt atcagctgtt gcaaaacagc attcactttt
     1801 tgtgcagtat cctttgaaaa gtctccttgt attccttgca ttgctacttc aggaaatttt
     1861 cgacaaattt cattgtatat cctaaaaaag atactatgga atgttccgat atggacagcg
     1921 gtttttctct ggctgaaatt atctactcgt ttttttagtt catttgcagc gttccttgta
     1981 aaagtgatca ttagtatgcg ttcttcgtct acttgttttt gcgtcatcag ataaacagct
     2041 tttgcacaga ttgtttgcgt tttgccgctc ccagctcctg ctatgattcg aagatgtttc
     2101 ttcatatcat atgtgatagc tgctttttgt tcttgattga agcgattata ggaacgtttc
     2161 tccatcattt taaagaaaga gctgtttcct tcactttctt ttacttgtac ttcttcctca
     2221 ataagtccgg gtgcttcgcc ataagccgct tcataggtag caatgatttc ttcttgtagc
     2281 cgaaagactt gttgaagctt tttatacttg ttctcccagc gtttcaattc tttttgtaat
     2341 cggagaattt ctttttcatt caactgattg atttccttcc catctttctt ctttgaattt
     2401 gtttatttca agttggttat ttaaagtttc gttgtctatt ttttctttat actaacagac
     2461 ctttctaaaa atgaaaatgg gaagcgatat gattttagtt ttctgaaaat ttttcctttt
     2521 tttcaatcgt ttatgctaag ataaggaaga caaaaatatt cataaaaggt gatgaaaaaa
     2581 atgcagactt cctttcacga ggggcgtaca ataggacttt tgctgacatt tatcggagga
     2641 gccatggaca gttatactta tatccaatat ggtgcttttg cctctgctca aactggcaat
     2701 atcattctat cgatcatcca agcatttgac ggagaatggc taagtgttgg taaaaaagta
     2761 ttgtctaccc ttttcttttt cttagggatt cttctaacaa agtatctaat tgattatttt
     2821 agaaaaaaag aaaaacattt ctggcgcttg tttgttctat attatgaagc agtgattttt
     2881 ttctttgtta gtttgacacc cgtccatgag catcccgcat tagtaacgat tttgattgct
     2941 tttactgcat cgatccagtg ggtagctttt gataaaatca acgggcgtgc ctacactaat
     3001 ttatttacaa ctggaaattt aaaaggggta gcaacgaact tatatgatta ttgtatttct
     3061 aaagaaaaag cagattttga tcgatttttc cactatctaa gtgtcgttct tgcctttatc
     3121 gcaggagcta ttgtccttgt tttctgtcat catctgtttg gcgcccattc gattttgatc
     3181 gtttccattt tatttttgat catggccctc actcagacat ttcatttatg gcaattttat
     3241 catacgaatc ggttcactat ttgatctcaa tgaatcaaaa aaactaagca agatgctgct
     3301 cacagagaaa tatctgcgag tgagcaattt gcttagtttt tatattattt agttgtaaag
     3361 attccctttg ctttcgggtt atttgtagtg tagcggatcg actgggcatc tgcctcttct
     3421 gtcattttct ttgcccgcat cttgatttct tgtacgattt gttctccaca tgccggatcg
     3481 gctacgaaat catatttgtc cccatcgatc actattttgg gacatatatc aaactgttca
     3541 taccactctt cgtatcgtcg attgagttct ttatagtagg aatagagttc aggatcttgt
     3601 tctagctgtt catattcccg gcctcttttt ttgattctcg ctaacatagt ttcaaaggat
     3661 acacgaatat aaaccagtag atctggtttc ttttgtggcg aaatttcttc taactccttc
     3721 agcatcgttt ctaaaagacc ttcatactgc tttacttcta cttcagaaac acgccctaaa
     3781 tctgcattca aatgaaacag catactatct tcgtagatgg agcggtctag tacgttgtca
     3841 tcatggctga aagcatcttt gattgccaaa aagcgtttgt tcaaaaagaa aatctgtaat
     3901 aaaaaagtat acttttctgg attagaataa aatagcggca atacttcatt atcttccacg
     3961 ttctcataaa aaggttttgt ttctaaatct tgggcaagca tttcagttag tgtgcttttc
     4021 cctgcaccga tcgttcctgc aacaatgatc acaattatcg gcatccttcc tgtaataaaa
     4081 tctgttcttc attttactac ttcatttata gaaataaaag ccttgacttt atctgctcct
     4141 taaaagctac ttctctaaaa gtagccaaaa gtaactccta ttcatgaaaa taaaggctgt
     4201 gacaaaagtc ttgtcgcagc ccttttttca aataaacagt attctcctgc caacacctcg
     4261 gtcacaaggc aatattcata aaacatgaaa agctgttttt taacattatt ccttgtaact
     4321 cagagctaaa cgggagactc acaacctctt tatgctctca acattttatc tgtttttatt
     4381 cttctattgt acttgatttg cgtacaacta actgccagtg cagattatga gcggtatcat
     4441 tgtcttctac tgcgcatttc atgacacgta tcatcacact attttcatag attttttcta
     4501 tttttccttt tattggtgat tcaaataaat cagatttaaa tgcatatgtt tcgccgactt
     4561 caaacaattg ttcattttcc ataagaatcc ctccatcacc ttttataagg tcagtaattt
     4621 tttaacataa atcactcgaa aaagcacaaa cttgtcataa aaagtacttt tcgtatcaaa
     4681 actgtcactt tttttggtaa ggttcttgga aaacgaagat ttttttagga gatcatgacg
     4741 gttttgatgt caaaaccact ttttatgaaa caaaagacgt tttttatttt ttttagcata
     4801 aaatttgtgg tggaggtacg tatgagatga aagataaagt tgtgaacaaa ttattagaag
     4861 ccttattcag cggagacaaa tcggaagaag atatggtgta tgtccaagta aaatttgtct
     4921 tagaagtttt attgaataat cttggaaaac tagcggtcgt tctagtcttt tctctggtga
     4981 caggctcatg ggcggaaaca ggaatcacct ttctttctta tatctgcatc cgcagatatg
     5041 cttatggatt gcattccgac tctgaattcg tttgtctgct gtggacacta ctttatttat
     5101 ggggcgttcc cttggtgatg aaacatctgc aattgactat ctccttccca atgatggtct
     5161 ttttgttaat cagctgcttt ttgttactgc ttcgctatgg atcaagaggt acagcaatca
     5221 atcccattga acccgagaaa cgtcccccat tattaaagaa ggcgatcagc atgtttctaa
     5281 ttttttctct cattacgtta tttttctcag cttcttactt ttctacctat ttgctacttg
     5341 ggattgtttt agaaatagct acgttgcttc ctattacaaa ctacttgatg aattttggag
     5401 gaatctttca tgaaaaaaac aatcattaaa caattaacta acttatctga cttttttgta
     5461 tctggctgct gctttggtat gagttctgaa ttcaaaatcc ctgaaaaatt aaaaaaataa
     5521 tacgccaatt gtgctataat ttggtaaaaa tcaatgggag ctgtacttta tgtttttatg
     5581 gtccatagcg ttgcaatatt tgatttacac aatttgtatc ttattgattg accatcacat
     5641 ccgatacaaa tatctgtttg cgggagtcat tctgatttct tgtattttga ttggatgggt
     5701 ctattcgatt ttagggattt ttactgctct aattgctata ggaatgctga tgcttacttt
     5761 tttcttcttt tcgacttcgc gtcaacaatt ggtttacgcg ctttcttttt gggttattga
     5821 cgtcgctttt aggagatcac ttaacaagca tcggcgattt ttttgttttg aaatcttcgg
     5881 ttattgaacc aggagattca ttgcagtatt ttcacattgt cttatccgct tttttatctg
     5941 ctttattagc tttttgttta aaaaaatcgt tagagacttg gtttcacact gtggaccgcc
     6001 gtatgatcgg tacgatcggg acattgatat tatttacata ctatatcatt attttttata
     6061 cccgtttttc tggtgaaaca cctgatgtct tagctttgaa tacgagtttt ttcattctat
     6121 atctgtgtgt tggcctgatc atcttggttt attattggaa aatctctaat aagaaattag
     6181 ctgatcagtt attagagcaa caagtcagga tgcaacagga ttatatcaaa gatctagaaa
     6241 aaaactatca agacttgaga gaattcaaac atgattacca aaatctgctt ttttctcttc
     6301 acagctatat ttcagaaggc gacttagaag gattacggtc ttattatgat caaacgatat
     6361 tacctacacg ccagatgatg aacctttttc cggccaatct aagtatgttg gataatatga
     6421 aagttcctga gatccgtagt ttactgtcat taaaactcat gatggcacag gaaaaagggt
     6481 tgatcgtcca gcttcatttt cctgagccaa tcgaactgga tacgaaacat acggtaaacc
     6541 ttgtacgtat gcttggtatt atcttggaca atgcgattga aggagctagt gctgcaaaga
     6601 agaaaaaaat cgaattgacc atactcaaga agaatcattc cgtgatgatt cgtgtgatca
     6661 atacaacaac aaatacatta ccgttaaacc agttgaaaca aaaagggttc tcaacaaaaa
     6721 caaatccaaa aaacgaaggc cttggcttgt ttatcttgga tgaattgaca aagaccaatc
     6781 cttatctttt ccttgaaaca tcaattgaaa accagcgttt ttctcaaacg atttatatac
     6841 aaataaaata aaggagtaat gatatgattc ccatttatat ttgtgaagac gatccaaaac
     6901 aattagaagt tttatcaacg gtgatcaaaa atagaatcat gattgaaaac ttagacagtt
     6961 atgtccatat ttctacctct gatccggaag aacttctcaa tgcagccaag atcagaactt
     7021 cttcttttgg tatttacttt ttagatatcg aactagaaga cagtgaaatg gatggtgtag
     7081 cgatcggtaa attgatccgt gaattagatc ctttaggaaa aataatctat gtgacttccc
     7141 atacggaaat gtcgatgaaa atcttaaaaa gcaacatcga accgactgat tatatcgtca
     7201 aagaagatct ttatgattta aaagaaaatg tcgagcatat cctgagcaaa attttcgaag
     7261 attttcaaat gtcccaaatc caaaaagata tcttcaaaat agagtttaat gatgagatca
     7321 aatttttacc gatcaaagac atccaatatt tttctacttc gctaggagcg ccacataaac
     7381 tggaagtcca tttgactcat gcccaattac aattctatga gaagatcaaa gaaatcgaga
     7441 aaatgcatcg gaattttatc cgttgtcata aatcctatgt tatcaataca caaaatgtgc
     7501 ggtcgattaa taaaaaaacc agagaagtca cgttggcaaa cggcgagacc atcccagcct
     7561 ctatccgtgg attaaagaaa ttgatttcat aaaaagaacc gaatagtttg gaccgactaa
     7621 atgaccagcc gatccaaacc cttcggttct tttttgccta tcagtgcaaa agcatttcct
     7681 gtgcaatttc acgtccatcc attgtatctg gataaaatgt tggccaattc tccatttctt
     7741 tcagcaatac ttcttgactt tcatttcctg catataaatg aaaatgttcg gagtcagccg
     7801 gtgcaatcgt gtgatcactg aattgaatat atctaaaagc gccactttcc ttatcttctg
     7861 cttcgaatag ataccttacg ccacggtttc ctgcctcgta ggtcaagatt tctttcccta
     7921 cataacgata atttgaacgt ttcgttgttc cattcttaaa cgtaaatgcc atctctttct
     7981 cactgatctt gattgaagca atatctgttt gatagccctt cgtataatat tctttatatt
     8041 cttgtgctgt cttatcctga ttcaattttt ctttatattc aaaaacagga tcaaaagtcc
     8101 catcttctac atacgtatct actgtttgcc aagttcctgc ccaatctgaa agagagcgat
     8161 cttgtacatc ctcgtcttca aaataaccgt tgtagacgct cttttctttt ttgggggtca
     8221 aagtatcttc tagcgggttt tctgtctctg tcgttttttt caacgcttcg aggtttgctt
     8281 ccatgataga aagatagttt tctccatttt ctatttgttc attcgtcaat ccttccaaag
     8341 gattcaatac ttctaaagat acacctgctt cgtttgctaa cgttctggca atactatctt
     8401 tggcgttttc ttcaaaataa atatagttga ttccatgttc tttaacaaag tgtttcagtt
     8461 cagcaattct cgccgcagac ggttcttcac tagaagaaag accagcaatc gatacttgat
     8521 tcaatccata ttctaacgct agatagttga atgcagcatg ttgcgtgaca aaagatcttt
     8581 gttttgcttg cttaaaggtt tcttgataaa gctgatggag tgcttgaagt ttctcggtat
     8641 aggctgctgc attttttgtc catacttcct gtttttctgg gtaagcttca atcagctgat
     8701 cacgtatgtt tgctacctgc ttgattgcta aacttggagc tagccataca tgtgggtcta
     8761 gctcatgtgt atggccttct tccccgtgtt catggtcatg atcatgtcct tcttcacttc
     8821 ccggaagtaa aagcatacct tctgtcgctt ttatcgtatg gacattgcct tcttgtaatg
     8881 ttttttggat tgcggggacc catgtttcca tgttttcatc attataaaca aagatatctg
     8941 tatcttggat ctttgccatg tctttagcag aaggttcata atcgtgggaa tcagtccctg
     9001 ctggaatcaa taactctact tctccttcat ccccgacgat tgcttttgta aaatcataca
     9061 tgggataaaa ggtcgtcatc acgctcaatt tttctttact gtcctcggct ttcccacttg
     9121 ccccgcaagc agttaacata cctgctaaaa ccagcatggc caatatacct atcaatcgtt
     9181 tcatttatga cactcctctt ctcatcttct ttcatttgaa tgttccgatt cgcttttcgc
     9241 aaaccgtaat agttacgttt tagcaacagg atttaattta tcatatggtt aaaagaactg
     9301 tcaagcgttt tcttatttta attgatgaga aagtttatat gaatgattga cgatattttt
     9361 gcaatcaacc tctatcattt tccttttata cccgcttttg tctataatga agttatagat
     9421 aaaggaggaa aaagagtgaa aaaagaaaca gatacaacga atcgtttgat ccgcttttta
     9481 ggaggaaaaa cctcttacta tgttttagga atagttatct tacttgcgct gatcatttat
     9541 tttttccatc agatctcgtt tatattcaag cctgtcggaa tcattatcgc tactatcttg
     9601 cccccattat tgtttgcact gatcttgtat tatcttttgg aaccagtcat caaacgtttg
     9661 agcaagcacc tttcgaggaa cttgtcagtc attatcgttt atgtgattat tttattttta
     9721 ttggggcttg gaagcatatg gcttattcct tttcttgaag aacaggcaaa gacattgatc
     9781 agtagtttgc cagatttgtt tcaagatttt caagagagtc tccgacaatt tttggagaaa
     9841 acaccttttg ccgattcatt taatcaattt ttcgcttcta ttgacgacgt gacgtctgat
     9901 atagctggat ttattggaaa ttattgggaa agtggcgcac aacggatcag caacattttt
     9961 tcaactgtta cagctatttt tattacttta tttactggtc caattgtcgc ttttttcctt
    10021 ttgagagatc ctcaaaaatt ttatcagtca gttttagcga ttgtcccacc agcatttcgt
    10081 acagatttta aaaatttgac aaagattgct aatcaacaat tgggttcatt tctaaagggg
    10141 caaatcattg cttcatttat ccttggagca gtctactggg tttgtttctt actgatcgga
    10201 ttagaatttg ctagtgtttt agctatttct gctgggttgt tatgtatcat tccatatatt
    10261 gggccgttta tcgccttctt tccaggtttg atcattgcat tccaagactc tacttttatg
    10321 gctgtcaaat ttgtcattgt ctggtttgcc gtacaacttc tccacggtga cttggtgatt
    10381 cctcgagtaa tgggtgatcg tttacagatc catccgatta cgatcttaat cgttttactt
    10441 gtcatgggtg atctaatggg aattgtgggc gttatttttg gtattcctat ctatacgtta
    10501 gtcaaattac tcgttacttt tattttccgt aaattcaaac aacgctacaa taaattttat
    10561 ggcgataaag gagagtatga aaactcttca ttttctgaag aagattattt taaataaaac
    10621 acaaaacgtc gatcttacga gaatagctcg taggaacgac gttttgtgtt tttgttttaa
    10681 tttttcagca aagcaattgc ctggtatggt tttagctcca gactttgatg aagaacagta
    10741 tcggaataat tatcgataag aatattggct tctgtgaatc cttctgggat ggcgatcctt
    10801 gtatctttat catagaaatt tgttaataca agcagctgct gtccttcaaa ttggcgaaga
    10861 aagccataaa cttccggatg atttgcttca taagcatgat aatctccttg tgcaacgatt
    10921 ggatatgttt ttctcagccg aatcaatttc ttgtaatagg acaaaataga atcttcagaa
    10981 gatagttctt tttctacatt aatctcttct agcttgcctc caacttttaa ccaaggtttc
    11041 cctgtagtaa atcctgcttg ttctgtgtct gaccattgca tgggtgttcg agagttatcg
    11101 cgagattttg ctttgatccg acgaaacgct tctttttctg tgaatccttg ttcaaggagt
    11161 aacttataag cattcaaact ttctacgtca atatagtcgg aaatcgatga atagtctggg
    11221 tcgatcatgc cgatttcttc tcccatataa atatagggcg tcccacggtt caaatggatc
    11281 atggttgcta gcatctctgc tccttgtttt cgatactgat cgatagctac aaatcgattg
    11341 agcgcacgtg gctgatcatg attattaaaa aatagagcat tccagccgtt accctcactc
    11401 atcttttctc cccatgtatg aaatagtcgc tttaattctg caaaatcaaa tggaggagta
    11461 gaccatttat tcccattttc ataatcgact ttcaaatgat ggaaattaaa gaccattgat
    11521 aattcttctc tactaggttc tgtatacaag atacaatttt cgatcgtcgt agagctcatt
    11581 tcaccgactg tgatgatagt ggaatccgtc ccaaacgttg cttggttcaa ttcttttaga
    11641 tacgtatggg tgattggttt gtccgtatat tcaggtttgc catcattttc tttattattt
    11701 ttcaattgtt cgtctttacc gataacattg ataacatcga aacggaatcc ttttacccct
    11761 ttttccatcc aaaaacgaac aactttgaat agttcctctc ttactgcttg gttccgccag
    11821 ttcaagtcag cttgtgtccg atcatataag tgcaaataat atttccccgt atctccaaat
    11881 ggcgaccaag cacttccacc aaattttgat acccaatccg ttggctcttc tcttaaaatg
    11941 aagaagtcct gataatgctg gtcccctgct agtgcttttt tgaaccattc atgttctgtc
    12001 gaaacgtgat tcagcaccat atcgagcatg atttctatcc ggtatttctt tgcttcggct
    12061 attagctgtt cgaattcctc catcgtacca aagataggat cgatggctgt gtaatcagag
    12121 atatcataac cgttgtcttt ttgagggctt ggataaatag ggtttagcca aatcatgtcg
    12181 atgcctagat cattcagata cgggagtttt tctttgattc cttttaaatc tccaattcca
    12241 tcaccattac tgtctttgta agatttggga tagatttggt aaatgacttt gtctttaaat
    12301 gtcatggata tttacctcca ttcaactgat ttattaccta ccacaactta tatatacaag
    12361 ttatcattta ttattataac tgaaccgaaa gcgtttgtaa aatttatctt tatacattct
    12421 aagaggtctg ttgcccaata aaaaacaaga gatcagacat gtatttgtct atcctcttgt
    12481 ttaaaatagc aaaatattct tgtttcttct ataaatggtg ttcaaaagct tttatctacg
    12541 gcaatcagtc ttctcaaatg taaaacatga ggagctaatt ttatttgagt attctttttg
    12601 ctcgatgcag gacggctttt ttacaacctc tgattcatgg tgttcaatca gctgtccttc
    12661 ggtattagct caaaaaacgg caaaatatga aaaactattt tgccgtttgt ttctctaata
    12721 ctcaggtcaa accgctgatt tcacaacctc tgtttttatt cattcgattt caacgcatca
    12781 atcatatcga tatgctttaa tttgaaatgg gtaacaatca tcacaataac cgtaaatacg
    12841 atagttaatc ctgctgataa gacataagcc caccatgtga tcactaatgg aaagatgaca
    12901 ttctccatag acgcttgttg caagatgaag tcagtcaata tatatcctac accaaatcct
    12961 ccgatgattc ctaaaagagt aaagatgata ttttctcgaa caatgtacat cgtgacttct
    13021 ttatcaaaaa atccaaggac ttttattgta gataattcgc gtacacgctc ggaaatattg
    13081 atattagtta gattatacaa gacgataaaa gccagcagtc ctgataatac gacaaagatt
    13141 aagacaaccg agtctaagtt cgccattgat tcttcttgcg tttcgatttg tttggacatg
    13201 aaagacgtat ttgtcacagc gtctgtggca agcagtttct cggaaagcat ttcttcttct
    13261 tttgttgtca tcttatctgt acgcagcaaa aatgcattat ccatcatcga ttgatcagta
    13321 actgaagcat aatattcagg ggacatataa ataaagctac ctagatagtt tttcacgatt
    13381 tcttttactt cgactttcca ttctttttga tcttcatcat aaagcgaaat atgatctcct
    13441 gtagataagt caaatagttc tgctactttt tgcgtaatag cgattcctgt tttaggcaag
    13501 gagcgtgggt tctcttcaag atcagttaaa tggataaaac gatccaaatc agctgtttta
    13561 cttggtacga tcactgtcag actttgcgaa gcatgtcctt tttttctcaa ttctacggtt
    13621 tgagtatgaa tagctaggat ctcatttact ttttcttgct tctgcagcat atccgctgct
    13681 ttttgacgac tatcttccgt ggatgaatcc aatgtaacga ttgcttgata gtcaatgatc
    13741 gttccaaatt gtttggctga aacagaactt agagaatctt tcaatcccac gcctgcaacc
    13801 atcagcccgg cacatccagc aattccgata atcgccataa gcattcttga cttatatcgg
    13861 aagagatttc ggtaactgat tttttgatta aagctaaggc gtgaccacag cggagtgatg
    13921 tattcaagaa aaatgcgttt ccctggcttc ggtgctttcg gttgaagcaa tgcagcaggc
    13981 cgttctctta aatctttgaa taaaacgatc attgcagcgc caaatgcggc aatgaagaat
    14041 gcggcagctg cttgtaaaat cggcggccag tcataaaaaa taagcgcact cccaatatca
    14101 taccgctcgt tagacagctc aaaaatgatc cttggcaaaa gattcgttcc taatatcgtt
    14161 cctaaaacaa tacctatccc tgaggctaga cttgcataga ttgcatattt ttttgcgatt
    14221 tcaactttcg tataacctaa cgcctttaat gtcccgattt ccctgcggtt ttcttctacc
    14281 atacgtgtca ttgtcgtaaa ggtgatcaat gcggcaataa agaaaaagaa aacaggaaaa
    14341 acattggcaa tcgcagcgat acgatcagac agatcaccaa attcttgaaa accgacattt
    14401 tctgaacgtt cattgtatag gtatgatggt ttagttaaat tttcaatttt ttctgcattt
    14461 cgattcaatt cttcctctgc agaggccagt tcacgttctt tctcaactaa ttgcctttct
    14521 tgttcagcaa gttgagcttc ttgttgagat aatgtttgaa cagcgattgt tcgttgcgct
    14581 tcaggaagct gctggatagt tgctgactgt tgcgctaact gttttttagc atcttctaac
    14641 cgtgttcttg cttgcgttgt ttgtgctttg ccatccgcta ttttttgctg tgccggttct
    14701 aattcttcgt tggccgattt tttcagctct tctaagcgtt cttcgggtcg atcttgtaaa
    14761 tacttttctg ccttttcttg attttgctcc attttatctt tgtaagcttt gctgtacgta
    14821 ttcaaattct ttacgtttgt aaaatcaaga tataagacag aataaatatc tgttttaaaa
    14881 acggattcag gtaaatagac aaaataatcg acgctaccat ttccgacgtt cgcgtatcct
    14941 ctctccaaat tgttgatgaa caatggagaa cggacaaagc ccgtgatggt atactctttt
    15001 tctttcaatt catctgaatc aatctgataa atatcaccga ttttataccc ttcatcttcc
    15061 gctttttcat ccaatacaag ctggtttggt ttttccggga gttttccatc agttaattgt
    15121 agcttgttct gcttgttatt tttgttataa gaaaatattt gaatcacttc gtttttgcca
    15181 gtattggaat aatagatctg ttttcctgtt tctacttttg catctgggat cttttctgct
    15241 tgagcaatat ctttttgtgt caagccacca gtagaaacga tttgcaaatc agaaagtcct
    15301 gctgtaccaa catagtgatc catcgttttt tgcatgacag gtccagcagt tttgacacct
    15361 acaaataaaa gtgttcctag catgacaata aggatgattg caatgaatcg tcctttagat
    15421 tgccgaattt cgcgaaaacc tgctttaaga tatgttcttg ttttcataaa atccccctac
    15481 catacaatct catcaatgga tgaaggatga tcatttaatt caacagacct tactttagca
    15541 tcattaatgt gaatgactct atcagcgatg ggcgctaaag cagaattatg tgtaatgatc
    15601 aaaacggtat tgccgtgttg tctgctagcg ttttgcaata atttcaatac ttgcttgccc
    15661 gtttcaaaat ccagcgcgcc tgtcggttca tcacataaaa gaagtttggg atttttagct
    15721 agtgcacgag caattgaaac cctctgctgt tcgcctcctg ataattggga cggaaagtta
    15781 ttcaatcgat gagctaggcc cacttgcctc aatacctcta caggatcaag cgcatcagga
    15841 gaaacttccg ttgccaattc cacattttct tttgctgtta gattaggaac gagattgtag
    15901 aattggaaaa cgaaccctac atctgttcgt cggtaagcag taagctgttt atctgaaaat
    15961 tgcgcaatat ctgtgtcatc aataatgatc tgaccttcat caggtgtatc catcccacct
    16021 aaaatattaa gaatcgttga ctttcctgct ccacttggac caagtataat cactaattcg
    16081 cctttttcaa ttgaaaaaga aatatcgtca tttgctgtaa tggtcgtttc tcccattttg
    16141 tatcgtttgt attcattttt tacttcgaca tacttcactc tgattcctcc attttcacga
    16201 tatttctcgc tatatctatg catcgaatgc gttttctcac acttatttat tttaccactg
    16261 aatttctcta gactaaagaa aggaatcctt taatttagtt gacaaataaa cgatttatga
    16321 tttacttaca taaaaaacat tgttatttga aagaaggaac atcatgaatg atttaaagga
    16381 aaatatgatt tttgatttat caaaaactgc ttttgtcgcg atcgacatgc aaaaagagat
    16441 cgtaaataac ggtacactct ctccacacac tgcagcatct attctgacaa acaatgattt
    16501 gttagtaaga acattgaaac gtaccgctgc cttgaaagtt ttagtgaacg tagacatctc
    16561 cactttccct tatttgagtc aacaaactga tatgggtgga atgactgctt ctgtacctcc
    16621 agaattcact gatttacttt tgaaggattc tttgaaagat acagataata tgttgactat
    16681 cacaaaatac aatccaagtg ctttttttgg tacttctctt gatcttcaac ttcgccggcg
    16741 tggaatcgaa actatcattt taagcggtgt agcgacaaca aacggtgtat atgctactgc
    16801 attagatgcc tttcaacacg ggtaccatat cgtgcttgct gaagatgctt gcagtgatcg
    16861 tgacaaagaa tcccatcaat tgttcataaa aaagatattt ccgaaaactg cacgagtacg
    16921 ctcaacgaaa caaataatcg aagctatcca acaaagcaaa taagtactgg cacatgtgat
    16981 tttcatttac ttctttttga tttcatggga aaataaccac aggatttaga taggagtatg
    17041 aacatggtga aaaaaaagat cacaatcaaa gaagtcgcta aactttccgg cgtttcaatc
    17101 acaactgtct ccttgatatt aaatggaaat acggagaaat tcagtccgaa aactgttcag
    17161 aaagttttag ctgccaaaga agaattaggc tatcagcctg actattttgc tagacaaatg
    17221 attaccaagg aaacgaaaac aattggtgta ctcgttcctg atatcacgaa tccattcttc
    17281 agtacactga tgcgaggaat tgaggatatc ttgtataagc agaatttcgt cacgatatta
    17341 tgtaatgctg attctgatca tcaaaaagaa atcgaatatc tagccgagct cacacgtcga
    17401 ggtgtggatg gattcattat cgcgacttct gctgtctcta cagatgcaat caacgaaaat
    17461 ttgaaaaaac aaggccggcc cttcattgtt ttggatcaga aaaaatctga aggatttagt
    17521 gatgccgtac gaacagacga ttttcgcggt ggttacctag cgggcatgca cttgcttagt
    17581 ctaggacatc aaacgatcgc ccttgtctat cctgaaaatc caccggaaaa tgtccacgct
    17641 cggattgagg ggtttaaatc tgctcttgat gtttaccaaa tccctcacga ccaactgatc
    17701 ctcttaccta cacagttctc aaaacaaggt ggctaccaga tcacagcaga acttcttgat
    17761 tctgctgcaa ctggtgtgtt tgctttaaat gatgaattag cttttggatt atatcgcgga
    17821 ttagaggaag ctggaaaaag catccctgaa gactacagca ttattggcta tgataatata
    17881 gatatgtgcg agtatatcaa gccgaaactg acaacgatcg cacaaccaat cttcgaattg
    17941 ggacaaacat ctgccaagtt attgttagat cggatccaat tcccagaaaa agaatgggaa
    18001 gaaaaaagat tacctgtacg attagaaaaa agattttcaa ctgcaccctt gaaatagctt
    18061 tcaaggtgtg ctatattttt atcgaactat taaaacgttt tactaaaacg ttttaaaaaa
    18121 taattttccc atcaaaataa atgagatagt tggaggtttg taaaatgaac acggttacag
    18181 taattggaag tattaatatg gacactaccc ttcgtttaac caacatgccc aagccaggcg
    18241 aaacgatgca tgcgcatgag atttttcacg ctggcggagg taaaggtgcc aaccaagcag
    18301 tggcagccaa acgttcaggc gcacggacat cttttattgg aggcgttggg gctgacagcg
    18361 aaggacaaca gttattagat ctactgacaa aagaaaacat cgatacatct gggatagctg
    18421 aaatccaagg cgccacaacc ggacaagcaa tgattatggt agacgcagca ggagaaaata
    18481 gtatcttgat ccatgcaggg gcaaataatg ctttccatga acaagaagtc ctaaaaaaca
    18541 agcaactgat tacaaacagt gactttatta ttgctcagtt tgaaagtagc ttagatgcga
    18601 cgatcctagc tttttctatt gcaaaagacg caggaaaaac aacgatcctg aatcctgcac
    18661 cagctcgtga aacgattcct actgaactac ttgaaaagac agatatcatt ataccaaatg
    18721 aaacagaaac agaaatcatt acaggaatca gagtgacaga tcataacagt ttggtcgcag
    18781 cagccgaaaa actccatgaa ttaggtattg gaacagtaat cattacacta ggcagtgccg
    18841 gcgctttcta tcatacagaa aaagaacacg gtatcgtccc tgcttttaaa gtggatgccg
    18901 ttgacacaac agccgcaggt gatacattta ttggcgcatt aagcagcaca cttcagccag
    18961 accttagtaa cttgaaagaa gcgatccttt atggaaatct agcctcttct gttgctgtgc
    19021 agtcttatgg tgcgcaacct tctatccctt accgcgaagc tttagaacaa tcagcagtct
    19081 aaaattaaca atgagaggaa gaaacaaaca tgaaaaaagg aaaagtaatc aattcagata
    19141 tttcacgagt aatcgcacaa atggggcact ttgataaatt aagtatcggg gatgcaggca
    19201 tgcctgtccc ctttggtact gaaaaaatag atttggctgt ctcaaacggt cttccaagtt
    19261 ttttatctgt tttagaaaac gttttagaag aacttgaggt acaatcgatt gaattggcgg
    19321 aagaaataaa gacaatgaac ccggaaatat tggaaaaaat caaattattg ttaccagata
    19381 caccgattag ctttatccca catgaagaaa tgaaaaaaga gttgaataac tgtcatgcat
    19441 ttattcgtac aggcgaaatg acaccttacg ccaatattat attagtgagt ggagtggtct
    19501 tctaatggaa acgacaatga atcctgaaca gatggtactg tccgagcaga ttcatatgct
    19561 gattgaacag ttgggaggaa aagacaatat tgattctgtc cttccatgtg cgactcggct
    19621 tcgtatcaaa ttgaaaaaac agccagtagc caatgcagaa aaattaaaac agcaaatccc
    19681 ttccattaag tatttcattt tactcgatag atatgcagaa ataatcatgg atcagggatt
    19741 ggctcctgct tacaaggaat tgacgcgtca aatcgatcat tcagctccga aagaaaagct
    19801 aaatccgatc gaaaaactat ttggtttgat atcggaaatc tttacgccga tcattccgct
    19861 gtttgccggt tctggtatat taaaaggtct ggttgtttta gcgactacga ttggctggct
    19921 tagtgaaaca agcggaactt atcatatttt gtctgctgcg tcgaatgccg tcttttattt
    19981 tctgccaatc tttcttgctt tttctgctgc caaaacgttt aaagtgaatg gatatattgc
    20041 ggctgtcatt gctgcagcac tgattgaacc gaatctgacc tctttacttt cagacagtgg
    20101 caaagcagct actgattttc tggggattcc tgtagtactg atgcagtata cgtctactgt
    20161 catgccggcg attttaggta ttttctttta ttcttttgtt gaaaaattcc tcaaaaaata
    20221 tattcgcgaa aatatgcaat tagtatttgt acctttactt tcattagtta tcacggttcc
    20281 tttgacgtta atggtattcg ggcctgcggg tgtttacctt ggcgaaggtt tagctgccgc
    20341 aattacttgg atgatggata ttaacggacc gatcagtggc gcaatcatcg ctggtggttg
    20401 gaatattttg gtaatatttg gtatccaatg ggctgtaaac cctgtcatga tcagtaatat
    20461 ctctgcttat gggttcgacc gaatcgttcc gttgacagga gcagccaatt ttggaatggc
    20521 tggtgcagct ttaggtgtat ttctgcgaag taaacgttca aaaacccgtt cgatctccgg
    20581 ttctgctttt gcttcgattt tacttgcggg agtgaccgaa ccaactgttt acggaattgc
    20641 gatcccattg aaaaaaccat ttgttgctgc ttgcattggt gcagctgctg gtggagctgt
    20701 aatgggattt gcccaagtga aagcgatcgc ttttgtattt ggtagtctga ccacattgcc
    20761 tgcattcatt tccggtactt ttttctggta cttagcagga ctggctgtta gtctagtcgt
    20821 agcaatgatc actactctcg ttagcgggtt tgatgaagac ctaatgtctt atgaatagtt
    20881 aatgaaaaag actgaagtat gagatgttta tcacatattt cagtcttttt ctttttagga
    20941 tcattatttt tataaatttt tgtgatactt tccaaacttt ggtataattt acatatgcat
    21001 aaccattttt atccccactt gtttatcccc aaacaaaggt ggtctatgca ataaaatgaa
    21061 cgtcaaagaa aaacgaatga atgctcgttg cttatttaca aattgtttta tttagaatac
    21121 acagacagat ttatgaaaag aaaaagcttt tcgacacgat tatctgttcc aaaaaatgtc
    21181 taatgaatgc tgaataagac agaaaatcaa tgaaaggaaa aggactagtt tattatgaaa
    21241 aataaaaaaa cgatacgttt gctagctgct acttggctga ttgctccgtc tgttttgtcg
    21301 accaataccg tatttgccgt agataatcaa atagctgatc aaacagtcac aacagatacg
    21361 actacatcca gctcagctac tagtgaaaca acggattctt ttacagaaga cccaactgga
    21421 acaactaact caacaacagg atcgaccgag ccaactgaaa ttgatccggc tgatccatca
    21481 gaaaatgaaa agccagccga gacatattcc cttacaattg ctccttcttt gattggtaag
    21541 atccaactga ctgcttcgac agcggattcc cctgaacagc aacctttgga tattacggaa
    21601 agtggaacgg tagaaggttt attagcggaa acgaaggtaa cttacactat tacgccagct
    21661 gaaggtgaac aaattgcttc agtcgctgtt aatggtgcca aagaagtaaa ttatgatgca
    21721 aagaccttta cagtaggtac gaacaattta tctattactg cagaatttaa agctggaccg
    21781 gatacttcaa cagagaaacc aactgatcct agtgaagaag tccctagtac accagatccg
    21841 gaaacgccaa acaaaccaga tgtgggtaat aacaacaata ataatggttc aaataattca
    21901 aacaataata acaataattc gaacagcggg aattcttcga atcaaacaaa cccatctgga
    21961 tcgaataaca atggaaatac tcagagagaa ccaaaccatc caaacaatgg ttcatcgaat
    22021 ggcggaacga acaatgcttc atctcgtcca aatacagcaa atcaaagcgg tatagagaat
    22081 ccatcttctg cttcttctga ttttgttgta aaatcaccaa ttgatgctgt attgcctaca
    22141 aatacaacga atgtccaaca agcaatcgtt cgagaagcat tcaagcatct tggaaaacca
    22201 tatgtatggg gagcaaaagg tccaaatact tttgactgtt ctggtttgac ttattacgta
    22261 tatatgaaag caaccggtca ttatatcggt ggctggacag gcgaacagca atatgcaggt
    22321 acgcagattc cagtaagtca ggcccaacct ggcgacctcg tcttctgggg accttcttcc
    22381 ggtgttactc accatgtagg tatctatatt gggaacggac aatttatcca tgcgcctcaa
    22441 ccaggagata aagttcgagt aacttctatt tcagatttca cgcctgattt tgctgtccga
    22501 gtaaacttag caggtttacc tgctgcttca ggttcgctga caggcggctc atctatttta
    22561 gatggattag atggctcgtt ccatttcagt caaaaccaaa caactgatca gttcttgaaa
    22621 aagattgcag atgatgcaca ggaaatcggg caaaaagaag gaatctacgc atcagtcatg
    22681 atggctcaag ctatccttga aagtggatct ggaaacagct tattatcaag cgaaccaaat
    22741 cataaccttt ttggtatcaa aggatcatat aaaggaagca gcgtgacctt caatacactt
    22801 gaacaagata gttccggaca aagctaccaa atccgcgcac aattccgtaa atatccttct
    22861 tacaaggaaa gtttggaaga ctatgccgac ttgatcaaaa atggactgac tggcaatcct
    22921 gatttctaca aaccaacttg gaagtcggaa acaaaagact acaaagaagc aaccaaatat
    22981 ttggaaggac gttatgctac agatcgccaa tattcacaaa aattgaacgc gatcatcgaa
    23041 gcgtacgacc tgacaaaata tgatgaaccg aaaaaagaag cagcaacaac tgaagaagca
    23101 aacgctgaaa tcctcaatat gtctaagcga ttcgatgtgc caatcaaatg gacaacagat
    23161 ggcgtgccaa tcacaggatt agaattttca acgataaaac gcgtcaacaa cgcttcttcc
    23221 atcttccgtg tcgcttcaat ctgggatttg tggaacaatt ttacggcaag acagatacca
    23281 gaaacaacta ctcgtacagt taaagctgca actgttcctg tactaacctt gttaagttta
    23341 gacaaatcat tctggcttga tctgccttta aagaaataga ataaattaaa aaggggatgt
    23401 gacactgtca cactcccttt ttaatttatt ctatttcttt atatttattt tgtattccgt
    23461 tacaaactcg cgtacttggt taggttccag agtgacatct ccaaaacttt caagtcgctc
    23521 cgctcctggc agttcttgag tctcaaatgt gataccgcca tgatttacta atttattccc
    23581 gcgcatttcc ggtccatctt ctccaaaatt tgctgtaaaa atcacgattc ctgaagcatc
    23641 tgtttttact tggatcgata ctttttcatc tgggcttgtc aattccgcag catttagtaa
    23701 accagattgt tttttcaaca aaaatggatg atcgatgccg cccactatca ttgattggcg
    23761 aaaatcactg gcaaagacct cacctaattt tttaggacgt tggaagtcaa atgctgtgcc
    23821 acttactgaa actacttctc ctgtaggcgt tgtatcttta ttgagcgtag caaataatcg
    23881 gctattcaac caaagtatat gttcgtcgat tgactgtgct ggatcaccgg ttaaattgaa
    23941 atagacatga tttgtaggat tgaatactgt tttgcggtca ctgatccctt ggaccgtcac
    24001 tcgccatgta tcatcgttcg tcagcgtata ccgtacttct acatctaaag ttccaggaaa
    24061 tccgttttcc atatctgggc tagtctgata gaaaatgacc gatgcttcgt tttcaccatt
    24121 gatgactgta taatgccact gcttggcttc aaagctaggt gcaccaccat gtaaaccaat
    24181 gaccagtgtc cgggtctgtt tttacttcga acgtttcatc atctaatttg aatgtgccat
    24241 tcgtaatccg tccagccata cgtccgattg aagcaccgat ataagggtct ttttctagat
    24301 attcttttgc ttcatcaaaa cctaagatca actctcgttt ttcattatcg attattgttt
    24361 gaaaatgaac aacccgtgcc ccaagatcac tgacagacaa agatgtccca ttttgattcg
    24421 tgatcgtaat caagtgatag ttttcgccaa aattttgttc attgatttcc atgttccttg
    24481 caccctcttt tctttcattt gcactgattc cagcatacat ggaaaccgtt ttcttaacag
    24541 cttattccgt gagtaacgag aaaaaattta ctatattcta ataaaagacc gtgaaaaaaa
    24601 ggttgtgaca aaaatattgt cacaacctct ttttccgaac aaacggtgtt caaaagcttg
    24661 catctgcggt aataagccca accaaacgaa aaatatgagg agctattttt gcttggctgt
    24721 tcttattact tgatgcagga cagctttttc acaatctctt tttcaatacg attccagtat
    24781 ataaccatgt cgtcagtaca tagtagatga aatacaatac gaagaatatc gtaaacggaa
    24841 cccaaatatc tttgtatgga ttttgcatga atagaccaaa catttgtaat ccgaataaga
    24901 catggactac acctaagatt cctggtgcta agaataacac gcctacttct ctacggatcg
    24961 attgtttaag caacccgcgt cgtgttccta tcttttcaag catttcataa cgagcaatgt
    25021 cactcttgct tcctgacaag atcttgaaca tcaaacagct agcaagcatg gtcaagaaag
    25081 ctaaacctag gaagaatccc ataaattcaa aaccagagaa tagaccatta tacacttcat
    25141 acatttcata tttctggttg ctgtactcta ctttttgcaa agatggattg ttcattttat
    25201 tttgagcaac aagtgtttcg attggtttca ggtttgcttg gaagtctttt acctgaacta
    25261 gctgtaaagt ggattgtggc aagttcagtt gatcaaattc tgattgactg actaccttga
    25321 tttctttttc ttgctgttcc ggcaattcat agcttcgaag ttcctcttgt acagtcagtt
    25381 cattcatttt tttagcagga actttttctt tcgtgatctt cggcggtgtt ccatcttcat
    25441 gttttatcat gatcaatggt tgagccgcaa attcacttgc attatagtaa atcgttcccg
    25501 catcttcttt ttgagtataa gaagcagaaa gtgttggtga aagttctttg attttttgct
    25561 gatcttctgc ccgaggatta ttcatcacta gatcatatcc agaattgttt tctgtcaagt
    25621 ctgcaatttc atttttaaaa cctaatccta ccgtcaaagc gcccagtgcc aaggcaaaca
    25681 gcatagcgac cattgagagc atttgtgtat attcacgaat acgaaagttt aactgtgaca
    25741 atgtgaaatt gttcaatttt ttcaatgaaa tctgttctga tttctttagt aatgataaaa
    25801 agaaaatgat cacagaatgg aaaatgaaat aagttcctaa tacgatcgta actaaggcta
    25861 cccctatacc aaagacttga taggtcccca cgtgtttcat catatagtaa ccgcagccta
    25921 gaaaagcaat tcccagtact acttcaagga aaaacaagaa tttatgctgc ttgattcttg
    25981 ttggtgtctg atccgcacga atcaatgcta aaatcggttt tttgacaatc gaaaaagcat
    26041 tgacaacagc agctaataaa aacaaaacag caaaaaacaa aacagtgatc aacaacccgt
    26101 gaatactaaa aggtgtaaaa tgcgaaatat tgagatctaa ctgatccact aataatcggt
    26161 ggacgatact tgtcaatcct acaccgacag ctgaacccac taaagtagct gcgatcccta
    26221 ctaagaatgt ttcagaaaaa atcatctgtg cgatttttct gccttttgct cccagcatca
    26281 taaacattgc atagtctttt tgccgcatcg tcatcaaaaa ggaatttgca tataaaatat
    26341 aaacgaatgt gatgatccct agtaaaacag aaccgaatcg gaaaatgata actgtagccg
    26401 caacgatcgt attacttttc aaaaaagctt cgttgcttgc cattgattca aacatgtaaa
    26461 aaatagcaga tgccataact aatcctgaga ataacaccaa gtagtctctt aaacggctct
    26521 tgatcccagt aagtgataat ttccaaagca tagtcaatct ccccctactg ttccaagttt
    26581 ccaagaatag atagtatttc tcgatagaag gaatcacggc tttgatctcc acgtttcact
    26641 tcttgatgga taccgccatc cttgatgaat aaaatacgtt ggcaataact tgcagaaaac
    26701 ggatcatgcg tcacaagaag aatcgataca tgatcttttg tattcaattc gtccatcgta
    26761 tctaaaagat cccgggcact tttggaatcc agcgcacctg taggctcatc ccctaataga
    26821 atcgttggtt gtgtgatcaa tgcacgtgcg gcggcgaccc gttgtttttg tccaccggaa
    26881 atctcagaag gatagctttc caaaatatga gagatggata aacgttctgc aattttttgg
    26941 acacgttgct tgatttcttt tggtctgacg ccttgtaaag aaagaggaac agcgatattt
    27001 tcttgtgctg tcaagctttc taataaattg aaatcttgga aaataaagcc aatctcattc
    27061 gcacgaaaat ctgctagttg atttcctttt aatgtggtca catctttttg attgattcgg
    27121 atatgcccat ctgttggttt atctaatgtg gataaaatat tcaaaagtgt cgacttacca
    27181 gaaccagaag cgcccatgat cccaataaat tcgccttttt ctacatcgaa gctgatgccg
    27241 cttaatgctt gcgtcttttt ctcattgttt tttccgtata cttttgttac attctgtact
    27301 tcgactattt tgttcatttc tttccctacc ctttaattag tttttccagt gctgttttcg
    27361 gcacgtcttt tttttcgact tcttcatacg aagtgacacc tttatttttg ttccaggtga
    27421 tttttaaata tgctccttta cgaagcggtc gagtcaattg tcccttgaag tccagttctt
    27481 tttccttacc ctctttcgta tagccaggca ggatatatct atagttgact gtttcttgtc
    27541 cagagtctga ttttgaaact gtttttgtac catctgtcgt aatttgtaca tagtattctt
    27601 ctccacccat aactacgtaa tctgcgattt tcacccctgc aagtaccaca acgatgaaaa
    27661 caagtagacc gatcagaagt tttttcactt tttccccttc tctcttactt tatatactga
    27721 ttatagaaaa aagcgaacaa ttctgccttc gaatcaacta tcattcttct ttcaattttg
    27781 tcattttctt taaaacaact aaaaaaagag ttcaagacaa taatccgtcc taaactcctt
    27841 cttcctatct tacaaaaagt atgtcactta cggtctttca tgtcgcctgt attgataaca
    27901 ggttgcgttc ctttgtctgt aatgattgag acaagtaaat tattgatcaa ttggactttc
    27961 cgatcatcgg tgaaattgat ttcttgtcca tcttcgattt gctctaaggc catttgagtg
    28021 attgtgacag ctccttcaac aatggtttgt cgagcagaca aaatggcttt tgcttgttga
    28081 cgttggagca tggcactagc gatttctgtt gcataggcta aatggttcaa gcgtgtttcg
    28141 attacttcga cacctgctac tgctaaacgc tcttgcagct ctttttgcaa ctcgtcagac
    28201 actgccgtcg tgtttcctcg aagcgtcaaa tcattctcat tgaatgtatc atacgggtat
    28261 tggctagcga tatgccgaat ggctgtctca ctttggattt ctacaaaatc ttgataatac
    28321 gctacatcga ataatgcctt tgctgtatca acgactttaa agactactac tgctgagatt
    28381 tcgatggggt ttccatccaa atcattgact tttaaaacag aactattgaa attccgaact
    28441 ttcaacgaaa cagtcatttt ttgtgccaaa ggtatcgtaa ggaaaaagcc attttctcga
    28501 atcgtaccta aatattgtcc aaaaaacaaa attacttttg cttgattagg actgactaca
    28561 gttgccgaac ttaacaacaa taacgtgatc aaccaaagaa aaatacttaa aaaaagtgcc
    28621 caaactttcg cttgccacat acctaaatag aagaaaaaca atccaactaa agctaatacg
    28681 accaatccga caatccctgc ataaccattg atataaaatg ctcttttttc tctcatgcta
    28741 cttcctgcgc tcctttatcc ttcaataaat atctaatgtt acttttttaa tttttttttc
    28801 gaatacgcgc cattacacct tctagttcta actctttgtc ttcagtgatg taccaaccgc
    28861 ttgcctgtgt ccctccggct gcctgcggta aataatccaa ttttgttcga acccagtgct
    28921 tttcttctgt aaaatagtct tctaccaaga cctctagatc catccctgct gaaagaggca
    28981 aatcttcccg aacagattta aagataaatc gttccttctt cccatcatac ttcaatattc
    29041 ctgtatcgat aatcggtaaa gaataataat ccagctcttt cttcaccgtc aataaatgtt
    29101 tgaaaacttc atggtacact tgctctaatt ggcgaccttc tgaattgttc gggtaattaa
    29161 ccatcacttt ttctttgtct ggatcataga aggtttggct tagttgagtg atataagtat
    29221 gtaatctttc catttctgtt tttaactttt ctctttgacc ttcttctacc ttcatatact
    29281 ctttcaccct gttttctttt tagatatttt ctttcttatt ttactatagt tatgtgcgtt
    29341 aagcgatgaa aaacagcaaa gattggaagg atctttgctg ttttttctta aaatatccag
    29401 ttgagtacag gatcaatctg tttcattgtc ctcttgatcc gtcgtaatat tgatcgatgt
    29461 gtctttagca gtgatcttta gctttttctg tccttgttct aacaaagtat ttgttattgg
    29521 ctggtagtct ttttcaatat aagatttacc agacgtttgg atagtatagc cactgttttc
    29581 catctcatgc aaagataaat tgccaccgtt taggatgact tttccttctt tgctgatcaa
    29641 actgtcttct agatggaagt tgctgttttc acctgtgacg atcaagttat ccacattggc
    29701 ctcatctaat cgaagtgagc ctaattcatc tttcgcagtt attgttcctc ctgtaaattc
    29761 attgaacgat acccagccat catgtgtctt agtattcact tttttgattg atacttcgtt
    29821 gatatcaatt gcactattat tcccttcgat tgtcaattca gataattcat gatctgcagg
    29881 gacaacaata gtgatttgtg cacttgtgtc ttctaaacta atgattgcac ctgttgattt
    29941 tttgactgca ttcacagtca acacgccatt tttctccttt atttctggct tttctgtctt
    30001 atcataagtc gtttttatct caaaatccaa tccgcgtatg accgaaactg tttgattttc
    30061 tgcatggatg acgattttgt ttgcatcctt gatttttttt gtctcagtaa ccatatcgat
    30121 caaccgagga ccattatccc aagtcagact cttttgagcg cctaatgtat aagttgcacc
    30181 tgctaggagg ccaccagtaa gcatcagacc aataccggta atcaaaaaat gtttcaatct
    30241 caatttatgt gtcctccttc aaacgcccgc tgaatgcaga tctctttttg taataaaacg
    30301 tcgtcccatc catttaacaa agttcatcat gacataaaaa agcccttttg ttcctttcag
    30361 caacagtggc gaaataaata ctgcgcttcc tgtggcgaac agaccgacac cgataaagaa
    30421 taatccactc agaatcgatt gtccaaacac actgattcct gagatgatca tgaataggcc
    30481 aacacctaag atactgatac ataaaacata gatactaaaa atgagcgcta gtaaagcaac
    30541 caatagtccc ccaataacca atataaaccc cagtgctagt ggaatgagga caggagaagc
    30601 aaataaaccg ataatgatta accaaatcag gcgaatttgt ctttttggat ttttcactaa
    30661 ctcgttttct gcttgttctt cttctgaaaa gtaactgatt tttaatttta aggcaaactg
    30721 ctttggtgta ccatattcat caatgatctg atctaatgtt tttccagaat ctaaaaaata
    30781 ttcctcataa tactggatca aatcaaaacg atcgtcttca ggaatttctt gaagatgatc
    30841 cacaacttca ttcaaatatt cattcatttg tgctctcctc caataaccgg tctaaagaat
    30901 ctcgaaaaac aatccattca tcaataattt cttctaaatg acgacttccc attggtgtca
    30961 acgagtaata tcgacggttt ctgccttcaa aaggttcatc ataggtctca agatatcctt
    31021 cttttttcaa tcttctcaaa accggatata gtgtagattc actgacagag agactttctt
    31081 gtacgatttt tgttaaggca tacccataca tatcttcctt gcttaatgct gctagcacag
    31141 taccatcaag aagaacagtt ggaatctgga tgtgcattac gtcaccttct attctcaagt
    31201 gtaatatttt atttcaatct atattataca acatataata tagtttcaaa atcagtttgt
    31261 caaatacaaa aaaactgact aaaagatttg atccttttta gtcagcaaaa aatggcttct
    31321 ctttgatttt tattataaca gtggcataat ccaatttgtc cacatccagc tcagtggcat
    31381 gataacgatc attgcaggta tcatgtcagc tactgggaac attttcacgt tgatcattct
    31441 aaaaccggta gcgaccatca agaatccgcc gcaagcttta aaatctaaga tcatgtctgg
    31501 tgtagtcaat ggataaataa attttgccaa taagaacaaa atataaaaaa taatgaattg
    31561 gggaattgct atcacagaaa cgacataccc taaattacac gcaaaaatag ctgctgtgaa
    31621 aaaatcaagg acagatttag aaatcaaaat cgtactgtct cctgtcatcc cagaatctaa
    31681 acttccatag atccccgtac cacttgcaca aaatagaaca atgaccgtca ctaatgtgct
    31741 gataaattca tcgtgggtca taccaagctt ttcactaggg aagattttag aaataggtac
    31801 ttgcatcagc gcagccccct tatttatccc attacctaaa tgaacaatca aaccaatcgc
    31861 tgttcctatc actaaagcaa aaataacagc cggcatattt ttcatcggcg caatggaata
    31921 gattcccatc cccatcgaac acacaccaaa aaccatcgtc agctgtgctt taaaatgttc
    31981 acttaattta tttcctacta atccgccaat tagtccgcca aacaaaacgg aacctacatt
    32041 gatcaaaact ccagttggca tcaaaaaact cctctctttt tctacgaagc gaaaaaaatc
    32101 agaaaaattt ctgtaattat tgctttcgct gcaagaaaga ttgtaacaga cagagaagat
    32161 tacaccaatt cattttttgt gttaaagtat tctttattgg aataagtagg aggaatatga
    32221 tggatatcca taaattcgaa gtttttcttg atttagccga aacaatgaat ttcaccaaaa
    32281 ctgctgatcg acaattcacc acccaaggaa atatttctaa acagattctt tcgttagaaa
    32341 cagaattaga cgtcaaatta ttcgaacgcg ccaatagaaa aatcgaattg accgaagctg
    32401 gaagcttatt gctcccatat gtaaaaaatg tcgtagaaca ataccatgct atgcaagaag
    32461 tgcttgaaac ctatacgaaa gacaacaatc tttcattaaa tatcttaacg ataccgacaa
    32521 tgatcaatta taaaggtttt tccaaaatca ccgagttttt gaaacgccat cctgaattta
    32581 cagtccagtt gaaagaagtg gaaagtgtcg aattaactta caatgacgcc aatgaacata
    32641 gtgacacgat ccactttgct agaagtttcc aggccccacc aagttcgatt gagtggttgc
    32701 ctactgaaac agacgatttt gtagcagttc tacctaaaaa tcatccttta gctagtaaaa
    32761 agaaattgga tctaagcgaa ctgaaaaagg agaacttttt gctccttggc cccactacga
    32821 acttgtatca acctgtacta gatctgtgtc gcgaagctgg atttgaacca aaaatctctt
    32881 ataaaggagc aagaatcgac ttgatcatca atatgattgc aaatgacatg gggattgcta
    32941 ttgttatgga aaaaacagtc aagaatctat taaaagaaaa tactgtgatt ctccctattt
    33001 ctccaacaac agagagttat cttgcattta ctcgtaaagt cggtgagcat tctttggctt
    33061 cagatacatt ttgggagtat ttaaatcaag aagaaaacta agccctacat tttctgtcca
    33121 tctatccacc caaaaaaaga gagcgacgca tgactttcgc tctctttctt gcatcatgtc
    33181 cttgtttcaa tgacacctct aacatttctc gtagttcaat aagaaagcag cgatttttat
    33241 gccaaaagaa ccgtaagaac atttccctaa tcacaaaaaa acgactaaaa acccctaaaa
    33301 accttcaata gaatttttag gggttttatt ctttttccta ttgtttactt aaccgtatca
    33361 ttgctgtcgt tcctactcct tcgatactgc tataatcaat acgtccatca tgcagttcta
    33421 gaattttttt aaccagtgaa agtcctagcc cattcccaga aatttgacga cttgtatctc
    33481 cttgataaaa tttttcaaac attcgatctc tggtctcttc attcattcct acacctgaat
    33541 cggtcacttc cagtacaatc tctgtggctg tctcaaatag tttgatatga atctgtccat
    33601 tgatttgatt gtactttatg gcgttatcca tgatattcag ccacacttgg tacaaaaact
    33661 cttcattgcc tgtgtaattt actgcagcaa gttcgatatc caattctagc tgttctttct
    33721 cccatttagg ctgaaaaaaa acgattactt ctctgatttg ttcatctaat cgatactctt
    33781 tcttttccag ttggatacgc tggttttcca gtttgttcaa ttttaagatg ttctctgtta
    33841 attgtgaaag ctgcgtgata ctttcgataa tccgatataa aaatatttgg cgttcttcgt
    33901 cagagagatt tggggcttgt aacaattgaa catatccttg gattgttgct agtggtgttt
    33961 tgaattcatg ggatacactg gacacaaagt cattcctcag tgtctcgata ctattgagtt
    34021 cttggaccat aacgttgaaa tctttataca gttgctgtac ttctgctact ttctgctgtt
    34081 catccattcg aatcgaaaaa tctcccgttg ctaccagact catatttgtc cctagcgcac
    34141 tgattggatg caggatacgc cgcccaacaa aaatcgatat tcctgtaccc acgaccatac
    34201 tgaaccccgc aaagacgaat aacgggtata acgttggttt tccagccgat tcagcaggcg
    34261 agatagaatg tcgagacaat aagaaaataa aaccgagaaa acacagcagt gtcactaaga
    34321 tcgaaatgaa gacaatacta gcaaaataga tccataattg tgaaaatagc ttttttttca
    34381 tctttaaata accgccttat agcctagtcc ccgtactgtg atgatttgaa aatcttccgt
    34441 cttttcaaaa cgggtcctta aacgtttgat atgtacatcg atcgttcgtt cttctgtatt
    34501 cgtatctaat ccccaaatat catccatcaa ctgttggcga gtgaagatct tgttgggata
    34561 agacagtaat ttatacagaa gcagaaattc tttttgaggc agaatcaacg aatgctgatc
    34621 ttgtaacact gtataagcat ctagctctaa ttttgtttgt ccgattatca agctactgct
    34681 gtaattgatc tttgaacgtc gaagcaatgc ttccacacgt aggatcatct cattgacatc
    34741 gatgggtttt gtcatataat catctgctcc taatttgaac ccgcgtttct tatcctcaaa
    34801 atcggccttt gccgtaatca ttagaacagg tgtgtcaaat ctggtgctac gtaatgagtc
    34861 tagcaagtca tagccatcca ttttcggcat catgatatct gtaatgatta attctatacg
    34921 tgtcgcttct agttttttca gcgcatcctc tccattcaat gcggtgatga ctgaaaagcc
    34981 cgctcgttcc aacacagaac ggtaaagcaa ctgtaattgc ggatcatctt ctactactaa
    35041 aatacgattc attgatctcc ctctgttttt tgaaaaattt ctagattttt tcttgtagtt
    35101 catcttcagt taacatttat ttcgtatact acgatcattg ttctgatata cttattagta
    35161 taccgaatat ttcaatacgg gagaagggtg attatgcttc aattaaaaga tataaaaaaa
    35221 tattataaag tcggcgaaac caccaccaaa gcattggatg gtgtgtctgt cgctttcaga
    35281 caaaaagaat tcgtagcgat tttaggtcct agtggttctg gtaaaacgac tatgctgaat
    35341 gtgattggtg gactagataa ttatgattcc ggtgatatgg taatcaacgg aaagtctact
    35401 aaagacttta aggacagtga ctgggatgcc taccgaaaca actctatcgg tttcattttt
    35461 caaagttaca acttgattgg tcatctaggc attattgaaa acgttgaact tgggatgaca
    35521 ttaagtggtg tttctaaaga cgaaaaacga aaacgggcag aagatgcatt gcaccgagtc
    35581 ggcttgacaa atcatatgca taaaaagcca aatcagttat ctggtggaca aatgcagcga
    35641 gtggccatcg ctcgagcact agccaatgac ccagatatcc ttttgtgcga tgaaccaacc
    35701 ggagcgttag atacagaaac aagtatccag atcatggaat tgatccaaga gttatcgaaa
    35761 gaaaaattag tgatcatggt cacacataat cctgaactgg cccatcaata cgcagatcga
    35821 atcattgaat tttctgatgg taagatctta acagattccc atcctcacat cgaacgacca
    35881 aaagatgatc aatttaactt acgcagaaca aagatgagtt tttggacagc gttaaagctt
    35941 tccttcaaca atatccgaac aaaaaaagga cgtactttct tgacctcctt tgcttccagt
    36001 attgggatca tcggaatcgc gatcgttctg tccctttctt ctggattcca aaaacaaatc
    36061 gataacacgc aagctgaaac gatggcgaaa tttccgatta ctatttctaa ggtaacgact
    36121 aaccagactc gtgacgatgc cggattgggt gcaagcaaag cggattatcc agacagcaaa
    36181 acgattactg caaaagtcag cgaagaagat aaagcacagc acacgaataa gattgatcaa
    36241 acatacgtcg attacgtaac agacatcgat cctaacttaa gtaataatat cggatttacc
    36301 cgtactacag gaatcaatct tttacgtgat gtaaatggca aagtacagcc tgtcagcttc
    36361 tcgaatcaaa atcctgatac ggaatctctt tctttatcta gtacgatgtc agcaatgaca
    36421 ggtgtgggag tgtcaagttt ccctacacag cttgacacta gcaaagaaaa ctttttaaaa
    36481 gacaattatt ctttacttgc cggaagttat ccagcctcag caacagatgt cgtgctgatc
    36541 gtagacggca ataataatac taatatcaac gcattgaaga atcttggatt tgatgtgaaa
    36601 gaagacgaaa aacttgattt tgatgatatc gttggtacaa cattcaaatt agtaaacaac
    36661 aatacttact atacaaaact tccaactggg aattttatcc ctaatacaga ttatgatgcg
    36721 atgtatcaga atgcttctga tgaattgaag atctcgggga tcctacgcgt gaaatcttct
    36781 tctacaatga atctcttatc tccaggaatc gcttatagcg atcaattaac gactcaaatc
    36841 gtcaacgaaa acaaagaatc agaaatcgtc aaagctcaaa gagacagtgg tgtaaatgtc
    36901 ttaactactg aaaaagttga cgaaaatgcc aaacaaacat tgcttagtta tttaggtgga
    36961 gacagcttgc cttcaagtat catgatttat ccgaacaatt ttgaagacaa agaaaagatc
    37021 ttggattatc tagatgatta caataaaggc aaatcagatg aagataaaat catttatact
    37081 gatttagctg gaacgatgac tgaactgact ggcggattga tggatgctat cacttatgtc
    37141 cttattgctt ttgcagggat ctctttagta acaagtatga tcatgatcag tatcattact
    37201 tatacatctg tgattgaacg gacaaaagaa atcggtgtac taaaagcttt aggtgcacgt
    37261 aaaaaagaca tcactcgcgt atttgacgca gaaacgtgta ttttaggtat ttcttctggg
    37321 atattaggtg tgtttattgc atggctagct acgttcccta tcaatagcat cttatacaac
    37381 atgacggatc taaaaaatgt tgctcaacta aatcctgtcc atgcgattat tctagtgatc
    37441 gtctctacta tattgacgat gcttggcggt catcttcctg cacgaatggc agcgaaaaaa
    37501 gacgccgcta tcgctttgcg tgcagaataa ggttgtgaca accacattta gatttgagtc
    37561 ataagagcgc tgaaatagta gaaaacagca agaaacgagg aagtaatatg ttccttgttt
    37621 cgcgctgttt tttttaatta ttttttataa ttctggtata cttttctttc ctttgtacca
    37681 caactcttct ggcgtatacc tgtcttttcc atttatgaat actgttccat ctgcttcaat
    37741 cgttacttca gtcccaatcc acatattacg agaaagtgcg agaagatttt ttctttttcc
    37801 tcttcctttt tttccaaatt gcaaggcatc ggttatctct tctgtccaat gagatttcac
    37861 gtattttact tctccgtttt caaatgtaac tttcaaaata ttcgctatat tttccacttt
    37921 tactgcttta ggaaatgact cctccatttt tttcactttc cttcctccaa cactaagtat
    37981 gcgctttctt ttagtgataa gtatatcata gtttattcta ttatcaaaaa aacgcatcca
    38041 ttccattgta gaatacttta ccgaacaagt tgaattgttt tcctcttttt caccttttac
    38101 tatagatagg taaagacaac taaggaggaa tctctcatga cagatcaacc gtatgattta
    38161 agaaaagtca tcgaagaact aaaaacactt ccaggtcaat atcacgaaac agatattgaa
    38221 atcgatcctg aagcagaatt atctggtgtt taccgttata tcggtgctgg cggtacagta
    38281 aaacgtccga ctcaagaagg accaactatg actttcaaca atatcaaagg tttccccggc
    38341 acacgtgtga acatcggaac aatggctagt cgtaaacgtg taggtgaaat tttgcaccat
    38401 gattatcgaa cacttggtcg tttcttgaaa gacgctgtag aaaatccaat caaacctgtg
    38461 aaagtagaca aagcagacgc tccagcacaa gaagtcgttc atctagctac cgatgcagac
    38521 ttcgatattc gcaacatttt gcctgcacct acaaatactc ctgaagatgc tggtccatac
    38581 atcacaatgg gtgtagtctt aggatcagat ccagaaaaaa caatgagcga tgtaacgatc
    38641 catcgtatgg ttctagaaga taaagatacg atcggtatgt acatcatgcc tggcggacgc
    38701 cacattggtc acttccaaaa acaattcgaa gccttgaata aaccaatgcc tatcactatc
    38761 aatattggtt tagatccagc aattgccatt ggcacgactt tcgaaccacc taccactcca
    38821 cttggctaca atgaactatg gtgtgcagga gctttgcgta acgaacctat tcaaatcgtt
    38881 gatggtatag cagtcgatga agtgggtatt gctcgtgctg aattcatcat tgaagcagaa
    38941 atcatgccaa atgaaacaat gcaagaagat atcaacacga actcaggaaa agcaatgcca
    39001 gaatttcctg gctatactgg ggatgctaat ccagctgtac aagtagtgaa agtcaaagca
    39061 gttactcacc gaaaagacaa tccaatcatg caaacaacga ttggtcccag tgaagaacac
    39121 gtatctatgg ccggtattcc tacagaagca agtattcttg ccttggtcga taaagctatt
    39181 ccaggaaaag tagtaaatgt ctacaatcct cctgcaggtg gtggtaaatt gatgaccatc
    39241 atgcaaatcc gtaaagaaaa cgaagcggat gaaggtattc agcgtcaagc agctattctt
    39301 gctttatcag ccttcaaaga attgaagaca gtcattttag tcgatgaaga tgttgatatt
    39361 ttcgacatga acgatgtagt ttggactttc aacactcgtt tccaaggaga taaagatatc
    39421 atcgtgatcc caggaatgag gaaccatcca ttggacccat cagaattacc agaatataat
    39481 cctggctcta ttcgtttccg tgggataagt gcaaaaacta tctttgacgg tacagttcct
    39541 ttcgatatga aagaacattt tgtacgtgca aaattccaag aagtagaaga ttgggaaaaa
    39601 tatttgaaat aatgaatgat ctgcaactgt tttgacatca taaaaaggag tgcggaaaat
    39661 ggaacaattg gcacaacgga agaaaaaaat cgtgattggt gtaagtggcg cttcaggcac
    39721 catttatgcc atcaacctag tgaagaaact aaaagaatat ccgatgatag aaacacacgg
    39781 aatcatcagt ccatgggcaa aacagaactt aaagctggaa tccgacttat cactaactga
    39841 attcgaaagc ctgtttgatt accattattc aaacaaagat ttaggtgctg ctgtcgccag
    39901 cggatcattt ttgaccgatg gaatgatcat cgtaccggca agtatgaaaa cagtcgcagg
    39961 tatctctgtt ggtttaggcg acaacctgat tgcacgtgct gctgatgtaa caatgaagga
    40021 gcaacgaaag ttagtcattg ttcctcgtga aacgccatta aatgccattc acttggaaaa
    40081 cttgactaaa ttagcacgac taggtgtgca aatcatacca ccaattcctg ctttttataa
    40141 tcagcctcaa acgatccaag atcttgttaa tcaccataca atgaaacttt tggatcaatt
    40201 tgaaattgta aatgagcatt caaaacgttg gcagggtgat taatatggaa aaagaaacga
    40261 tagttgaagt aaccaaacta ggtttcacga tccaagctgc tgttaaagta atcggtaaag
    40321 acttattgat cactttaaca ggcggagata caccacatat cggaacagtc actacatttt
    40381 ctcgtgatac agagatgcaa actgtacgtt tccctagcca cgatggtcgg ttccataaag
    40441 acgatgtctt agccgaaaaa atagctgtga tcatcaaaac tgccgttccg ggaagttgta
    40501 cgatcacttc cggtatccat gttgaccata tctccaatgc acaaatcaac gcctcttttc
    40561 cgatgtcaga agagttaggt tacaagattt tagattggtt ggaaaaaata gatttccata
    40621 tcaaagaacc agtttattat aaagatggtg aaaaaccatt atgaaagaag aggttgtgaa
    40681 atcagcgtta tgacctgagt atgagagaaa aaaatggaaa aatagcttct catattttgc
    40741 cgtttttgag ctaataccga gggacagctg attgaacgcc gtttatcaag aggttgtgat
    40801 aaagccgctt cgtttcgagt agtaagaaaa attttgtaaa catgggtttc cacatttttg
    40861 cataaatttg acttactacc gagaaactgg cttttgaaca ccgtttatca agaggttgtg
    40921 acacgtcttt tgtcacaacc tcttttattt ttgcttcact gtaactgcta caatatcacc
    40981 gatttctttt ccgatgtttt tacgaatatc ttttcgtagt ccaaggatgt agcagactga
    41041 accatctgga tttttcacgc ccatattcac gatactgcct tcatatggca cattgtcaaa
    41101 agttgcttgg actttaactc ttccagcacc gaattctttt cttatatcat acggaaaaat
    41161 aatatatgcc ccgcccttgc cgacttcgga agcatagatt tttgattcaa aagaataaat
    41221 ttccctcatt ttatttctcc tattttttac atctagtata gcatagcaaa aaaaagaagg
    41281 aactgtgaca aaagtcttgt cgcagttcct tttttcgaat aaacggtgtt ctcctgccaa
    41341 ctcctcggtc acaagtcaca atagtcaaca aacatgagaa gctgtttttt gaaattgctc
    41401 cttgtgactc agagttaaac ggcaggctca caacctcttc cttaaacggt gttcaaaata
    41461 ttattcgcct tttgcttctt tcactaattt tgcgaatgct tgttgttgga attcagcagc
    41521 taaggctttc gtacgttgtt cttgctctgc tttgctcatt tttggatttt cagtcattgc
    41581 ttttaccata ccgtcttgaa cataagcttt tgcttcactt tccatcgtac tttgctgttt
    41641 cttcactaat tcagctaact tggctgcttg gtccgtactt aattctaccc atgtctctgg
    41701 gattttgaat gtattcactc ggctgtcata ttcgtgattg ttatcagcaa tattgaacca
    41761 gaatttatac atgtcgtttt tgtattccca ataagtagtt ttcgtcactt tttcagcatc
    41821 gccgtctgtc ttttctactt tatttgtttc gttatccgta ccagttgctt ttggtttttc
    41881 ttgtttttca tttgttcggt aaagatacac tttttctttt ccattcccta aagctttata
    41941 caaaagcata tctgctcctt gggaatcagc actggaaaca attttttctg tttttgtttc
    42001 cgttactttt tccattccaa agtgattgga caaattagcg acgatcaaac ttagtgaagc
    42061 gacgaagatt agtccgaaaa tcaaagataa aaaggtttgc catgtttttt tagcgaacac
    42121 gttcgttaaa gcaaaagcaa gcaagctgac aatcaaaact ccaataatca tgcattatcc
    42181 cccgctttca ttagaacagg ttcttttttc ttcaataaaa aggcaataat aaagccaaga
    42241 atcccaaaga tgatcgctac gataaacgcg gcatggtaac ctgacagtgt ggcattgatt
    42301 gcctgatctt tataagctaa tggcgtatta tccaatacag atttgcttgg gatatttgct
    42361 tttgtcacat tcgtcaacac actgatcaaa atagctgtac caatcgagct tgcgatttga
    42421 cggaatgtat tatttaccgc tgtcccatga ctgatcaagt tatttggcaa tgcattcata
    42481 ccagaagtcg ttaccggcat catgaccatg gagatcccaa acatacggat cgcataaagg
    42541 atcacgatat ataaaactgg agtcgttttt gtcaagaaag caaatggtgc agttgccgct
    42601 gtcaaaatga acatccctgt gatggctaaa cgtttcgcgc cgtatttatc gaaaatcgca
    42661 cccgtcacag gcatcatagc ccccatgatc aatgcacccg gcaacaacat caagccagaa
    42721 tggaatgctg attctccacg aatattttga atatacaatg gtacgaccat ttctgcccct
    42781 accatcgcca tatttgttac accagctaaa acagctgcaa tcgtgaatgt agatgatttg
    42841 aatacacgaa gttccaagaa tggatgttcc aaatgtaatt gacgccatga gaacaaggcg
    42901 atgaaaatca caccaacaac taagaaacca attactttca agcttcccca gccgtcatct
    42961 cctacactag aaaatccgta aagtaaacta ccgaatccaa tcgttgataa aatagcggat
    43021 aaaatatcaa tgctaggatt tgacaatgga ataacacttt tcattaggaa gaatgctaaa
    43081 accaagacaa gaacaacgat tggcagtacc ataccaaaca agtcacgcca atggtagtta
    43141 tcaatgatat aaccagataa tgtagggcct aaagcaggag caagtccaat aacgatccct
    43201 gacatcccca ttgctgatcc tcttttttct ggcgggaaaa tagatagcat gatattttgc
    43261 aataatggca tcgatactcc cacaccgcaa gcttggatca aacgaccgat tagtaatgta
    43321 ctaaaattag gtgctgtaaa gcaagtaatc gttccgatca aaaagatcgt catggcagaa
    43381 agatataaat ttcttgaact gaatttattg atcagccatg ctgttattgg gatcatgatc
    43441 ccgttgacta gcaagaaacc agtcgttaac cattgcacat cagacgctga aatatcgaaa
    43501 gctttcatca atgtaggaaa agctgtcgtc aataaggtct gattcaaaac ggtacaaaat
    43561 gtaccaatta ataagacaac aactaaaagt gtccgattat atggttttcc gtaaatatct
    43621 acgggttggt tgttactcat atttctcctt ctttctgaat tttttctgaa actactagaa
    43681 tactttagtc ctaaattcgc tatttgtcaa cctacttgac acataagtta atacggatat
    43741 aattaaggga agcaagagga caaaaggagt gacaaaaatg gttggggctt cccttaaacg
    43801 tttgttggaa aatgatcaat tggttgttgg catcagtgaa ttaagtgata tcgtaggtgt
    43861 gtctccacgt caattaaggt attgggaaca aaagggattc attcgttcga tcgaagctga
    43921 tgcgaattgt cctagaaaat atcgtttacc tacagtcata aaagtagaac tgatcaaaaa
    43981 atatttagat gacggattta ctttgtcaaa agctgcagaa aaggcagaaa tccgccaaaa
    44041 gaaaatgcac catgtccgta aagttttttc aaaagcaatc aaagatatcg aattgatcga
    44101 cgaacgctat acgattttac aaatcggtaa cttcaaagaa gagaatgcta agatgtacat
    44161 cattcatgac aacgagacag acgaattaac ttacaagctc cttcctgtcg atgaaaaagc
    44221 tgactacgaa cagttgttaa aagaactcta aacgccacta atctcctaag taccaagctt
    44281 tttaataagt agatgctaca taaatacttg aaaaaaggcc tgtttgaaca taagaatatc
    44341 cgaacggtga tctgcgtttc tttcatatag aaaggaattc tccatcactg ttcggatatt
    44401 tttttatttt tttcggttac tttcttctaa tacatctgtt agccaatttg catattctgt
    44461 ttcccgattg atcgcatgcg ctaaaatcag gtagtgtccg taattttcat gtttttcttt
    44521 ttcagtagga aagatcgttt tttttcgctc taatagatga agtcgttttt cttcatgaag
    44581 gtcacgctgc tgctgaataa tttctactaa tgccggatca ttcacgtctt tgacgaaata
    44641 caatttcaga acaaattcgt ctcgattaac cggtagttca ttggtgggtg tatgaatcca
    44701 ctctgccaat tgttccttcc cttcttctgt taattcataa actttctttt ccaatttttc
    44761 acctgtgatc tctacttgat gctgaatgat tccttgctct tccatttttg caagttcagg
    44821 ataaatctga ctatgttttg cttgccaaaa ttctcctatt tcatttttga acgattgatt
    44881 caattcatat cctgtcatct tcttcttact taataaccct aacaaaatat atcgaagtgt
    44941 attttttctt gccatcagtt ttccctcctt gacaaatctt taaatccatt ctatcataaa
    45001 tgagtaatcg aaaacatgta gtttattaca tgttttaatt tataaggaga tgcttaaaca
    45061 tgaaagaatt taaagaattg gaagatttta ttggaactca ctttatctat acgtatgaca
    45121 atggttggga atatgaatgg tatgccaaaa atgaccatac tgttgattac cgtatccatg
    45181 gcggaatggt caaaggccgt tgggtaaaag accaagaagc tgatatcgtt aaattgacgg
    45241 acggcgtatt caaaatcact tggacagagc caacagggac agacgtggca ttggacttta
    45301 tgccaaatga aaagaaactt cacggaatga tctttttccc taaatgggtt gaagaacatc
    45361 cagaaatcac tgttactttc caaaacgaac atatcgcaga aatggaagca gctcgtgaaa
    45421 aatatgaaac ttatccaaaa ttacttgttc ctgaatttgc tacgatcact tatatgggtg
    45481 atgcaggtat agacaatgaa gatgttatta gcgaagaacc atacgatgga atgccagatg
    45541 atatccgtag cggaaaatac ttcgatgaaa attataaacg agtaaaataa ggttgtgaaa
    45601 gagaggttgt gaaatcagcg ttctgacctg agtattagag aaagaaacgg aaaaatagtt
    45661 cctcatgttt tgccgttttt tgagctaata ccgagggtca gctgattgaa caccgtttat
    45721 caagaggttg tgaaatcagc gttatgcttt gagtaataag aacgctagtt agaaaatagc
    45781 ttttcatatt ttctaggcta gtgggcttat taccgagaag cagccgtttg aacaccgttt
    45841 atcaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa tggaaaaata
    45901 gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt
    45961 tatcaaagat tgtaacaact gcatattcac acagttaaat tagtgacttc aaaatgaaat
    46021 aatgctccca gcaaaaaatt aggctgggag cattatttcg ttattctgtt catcaaaaat
    46081 cagcgttttg attccatttc atcacaagtt cctcctcatt tactgcttca tctttttgct
    46141 gtgtttgaat tgtaaaacct tgtgaaagat agaaatgaat agctgattga tttttttgat
    46201 agactgtcaa agtcagtgtt ttattggttt cttttatttt tttcattagt tgcgtaccga
    46261 ttctttgttg ccggtattcc cttttcacaa agattcctgc gataaactga tcaatcaccc
    46321 cagcaaaacc aataatttct tgatctattt ctgctaagta aatcactgca tctggtaaag
    46381 ctttcctaac agattctact tgattctccc aataagttga tggaatgaat tcatgagaat
    46441 ccaagttagc atgcagccaa atcgatagaa cagattccag ttctttggca gacaattttg
    46501 ttgttgtgcg gatcattttt tctctcctta cttattcatt gattttaaaa taaaaaccat
    46561 ttttttgaag ctcttttaca agttttcgcc cctcaaaaaa gtatatcttc tcttgtttta
    46621 cttttctaat accaaaagaa acagcaactt taggatcatc agctgaaacg attcgataag
    46681 ttccaccagg caattgatcc agcttgactt tcccatgatg gtcgctttgt actgaaaacg
    46741 gcacaccatt tctagtaagt gtttttttct tatgtttcaa aatgaatgaa ctaccattta
    46801 ccggtttccc tgacgaatca ctaataataa atgaaaaatc aagcactttt ttacttaatc
    46861 tatactgata gattagtcgt ccgataaaca ctaacagtat gactacagct attatcgttc
    46921 ctgcaagaat aaaccattgc cgcatggttc tttcctgatt tccttttcct tcttcttttt
    46981 ttaccgattc gttataagga atccgatgtc ctgtcactag caaacgatgc gtgttgatca
    47041 tatacggcgt acatgtaagc aaggtaacta agtcttctcc ttctgaaatc gttagaacag
    47101 acgtgtcatc tggcagtacc acttgtatat ttttaacttg ataagccaat ttctccccca
    47161 aaacagtcaa aataaacgtg tctccgtttt ctaaacgatc taattcggta aataaccgtc
    47221 gattaggtaa accgctgtga gcagagatca ctgaatgcgt cgaatttcca ccaagcggga
    47281 aagaggttcc ttgcaagacg cctgctccat tttcaaggat cctaccattg agccgatcat
    47341 aaagtgaaat atttaattgg atcttaggta tattgatact tccgatcagc cattcctcta
    47401 attggctgga tgttgtactt tcatttctgt tcttttcatt gaaaggatca gtaccaggat
    47461 tgaatccttg gtcagttaat cgttggtttt tctcctccat tttctttcgc tgcacttcaa
    47521 tttcagctgc ggtccgtttt tgtacttgtt cgatacgttt ttgatcaata aaactattta
    47581 aagaatccac ataaaatgga tataaagcga tcagtaaccc tacgacgata catattaata
    47641 aaaacaaacg aaacaaaagt cgacgagact gtttttgctt actctgcttc acatgtgtcc
    47701 tcccttgcaa gtttttttgt tctttctttt attatatggg ttcaggaaaa aaatagaaaa
    47761 tgaaagccga tgaggttgtg aaaaagctgt cctgcatcaa gtaataagta cagcccaaaa
    47821 aaatagctac tcatattttt tgttgggttg ggcttattac cgcagatgca agcttttgaa
    47881 caccgtttaa ggaagaggtt gtgaaaactg cgttatgctt tgagtaataa gagcgctagt
    47941 cagaaaatag cttctcatat tttctaggct agtgagctta ttaccgagaa gcagctgatt
    48001 gaacaccgtt taccgagagg tcatgaaaaa gctactttgt ttcgagtatt aagaaaaatt
    48061 ctgtttcaaa acaaggagat gcaacaaaac ttatgtcgca tcctctctcc ttagaacttt
    48121 cacttatgtt tttcttgtaa aattcataaa tattttcatg aattttctaa tttcttgcct
    48181 acctctcgca taggatatat gatgaattta tctaaagttt gcaaaggagt tcgacaattg
    48241 aaaaaatatg gcccattttt agcaattttt ttcattgctt tggcatttct catgcctcat
    48301 ttattcacac ataaactaat tttgggtgct gatgccccat tccattacaa tcggttctat
    48361 gatgccgcgc aacaaattaa atatggggat ttccattatt ttccttccct ttatggattc
    48421 cagcaaagtg gcaggatcgt gaatgccgtt tacggcccat ttttcgctta tttacaagga
    48481 ttgatcgtgt tgcttgcagg tagctggttt cattatcaga ttctttcaaa tttcattctt
    48541 tatcttattt caggattttc tatgctgatt ttactgagat atgtcaatgt tcgaaattgg
    48601 gtaagtgtac cagtagccgt tttattcatg agtacgtatt ctattcaata ctggtctatc
    48661 aaccaaggat tcactagctg ggggacttgt ttttatcctt tatgtatgat ccctattgct
    48721 gatttcgtcc ttcataaaaa gttccctgtt atcaaagtcg ctgtctcagt gggacttatg
    48781 acacagatcc atttactgtc ttcgatcatg ttagctttga tgtatatccc tttttatctt
    48841 tattactttt ttaaccagac tgaaaaagga aaagcactgc ttcaactgat taagtcggtc
    48901 ttgctttatt ttgttctgac agcaaatatt tgggcaggat tagctattgt ttacagtggg
    48961 aataagattc ttcctccctt tgttaacaga gaaatgagtc aaaaggcgat agatctggat
    49021 ggcagttatt ggctccatta ccctaaggcc tttcctatat tgatattatc aggttttttg
    49081 ctttttattt tctttcaaca aaagaacacc ttattccaaa aaatcgtgtt gtacacaagt
    49141 cttgttttct tttttctttc tactagcttg attccatgga cgacattgat cgaaaaaaat
    49201 gtgcctttta ttagtttgat ccagtttcca tttcgatttt tcgtgccatt tacgctattg
    49261 cttctacttt accttgccct ttcactgaac cagtggtctg aacaaaagtg gttccgtctt
    49321 ttaagtatat ttggcattct tattttttca gcacagacga tccagaactt ataccagcat
    49381 ctggaaaagt gggaaaatga aacatttgtc agtcgacata cttatctatt cgatacgccg
    49441 gaagaagcaa ggaaaacatt cttcagcaaa gatatgtcta ccagcttgtt cgttttccaa
    49501 aaaaccacac ctgactatct gccaatctat aaggagacaa aagacaataa atacgaccgg
    49561 tataacgaag aaatactaga aaacgaaaca acttttataa aaacacatcc aggcggtctt
    49621 ttgacaatca agtggaaaaa cactggggac aaaactgttc atatccctgt tatcgtctac
    49681 gatcggactg tgctacagca aaatggaaaa acattgactg attatgaagt aacagatatc
    49741 gggaccccaa tcgtgaagca gcaaaaagga atcaacgagc tcacgcttca ttatcaaacg
    49801 ccaatctatt tctatttcat tctttctctt actttgattg gctggtttac cttgctatgt
    49861 ttatttattt atcatagata caagctttta cgtgcttagg caaaagggat aaatacatcc
    49921 gatcaaaaaa ctctccaaac ggtataatcg tttggagagt tttattttaa tcagtttttt
    49981 tatgttatat gttatatttt ttattcgttc acatctaatt cgatttccac cccgtagtga
    50041 tcactgatga ttggactatc gatcccatca aaaatccgtt gatatttttt aacctgcatt
    50101 ccttcaggga cgaaaataaa atctatacgc aatttttctg tattttgttc ccaaccatct
    50161 attttctttt ccacagtagc tgcttttcct ttttcattcg cgacaacata cgaatcttga
    50221 agaagaaggc aactttctct tacagtataa taaccttgag agtctactgg attattgaaa
    50281 tcacctagga aaaaaagtgg tacgcgagtt tccaaaaagt agttttccaa gtttttccac
    50341 tcaaaataaa agccactttt tttatctgtc caccaagaaa agtggcagct cgagacaaaa
    50401 atgtcctgat catctatctt tgttttccca atcaagatct tgcgtgtctg ataatcttct
    50461 ttcttttgaa attgggagac ggtaaggacc tgactttcca atgttttttt cgtaagcagt
    50521 gcatttcctt cttcatattt atcataaccg atgtggctca tttcccaact ccaaaaatac
    50581 tcttttccgt gttcctttaa aagctgaact aaacggtagg caaaattatc tttacggata
    50641 ggtacttgat tctttaccgg acagaacttc attagcttcg caggttctac ttcttcagat
    50701 tccagtaatt ggttgatttc ttgtaaagca atcacttcat aatcttctaa caggatctta
    50761 tctgctaatt cttgcaattt tttctctggt tctggctcaa gccagctatg cgtgttgagt
    50821 gtcatgaatt tcattggttt tctccttgtc ttcatttcga aacaaaaggg ttgtgacaaa
    50881 atttttgtca caacctctcc agcaaatagt gttctcctgc taactcctca gtaataagct
    50941 caatggacca ggagctattt tttgtttggc tgtttttatc acttggtgca gggcagcttt
    51001 ttcacgacct ctccactaaa cagtattcaa actgctgctt ctcggtaata agcccaacca
    51061 aacgaaaaat aggaggagct atttttgttt ggctgttctt atgacttgat gcaggacagc
    51121 tttttcacaa cctctcctta cattttaatg gagccgatca ctttaccagc tgtcactgta
    51181 cctaattgct tgatcactac ttcttctact ttatcgtttg taaatacgat catgatcgtt
    51241 ggatcttttt cttcgttttg gatcacatca agatccatct cagctaattt cgttcctgtt
    51301 cctacttctt ggccttcttt gacagcaatc tcaaaagctg gctgattcat ttggactgta
    51361 tcgattccca tatggatcaa aacttcgata ccatctttcg tttccagccc aattgcatgt
    51421 ttgctaggga aaatacttgt cacttttcca gcgatcggcg caacaacttc tcttgatgtt
    51481 ggaataacag caaagccgtc acccatcatt ttttgtgaga ataccggatc ttttacttca
    51541 gataaaggaa tcacttctcc agaagctgcg gaaactagtt cttgttctat acctaagtat
    51601 tgcttactac ctgcttgtcc atttcctgta gaaaccattt ctggctctgg aatctctgct
    51661 cctgattgca gcagatcttc gatatctgat ttcaagacat ctgcttttgg tccataaact
    51721 gcttggactc cattgtcttt gacgatcaat cccatcgccc ctgctcgttt ccaggcttct
    51781 tcagtcccta ctttttctcg atcatttaca cttacgcgta accgagtcat acaagcatcg
    51841 acatctttga tattttcttt tcctcccagc aaatgaatga tctggaccac ttgctgatct
    51901 gctgttcctg ttccgctgtt agatacaacg gtttcttcac tgtcattgtc gtaatttccg
    51961 ttacgtcccg gtgtagcaaa gttgaatttt ttgatcaaga aatttgctaa gaaatacgta
    52021 acaacaccaa aagcgatcac acacaacacg aagttgataa gatctccacc tagacccgct
    52081 ttgatagcta acggtgtccg agtcagcaat tcgatattcc caaatgaatg gacccgcaat
    52141 ggcaagatat ctgccatcgc aaatgcagcc ccttggatca ctgaataaat gacatataat
    52201 ggcacagcag caaacataaa catgaactcc aatggttctg ttacacctgt tagaaagaca
    52261 gccaacgctg ctgaaaaata cattgattta tatcttgctt tcttatcagg atcgacatta
    52321 cgatacatcg caaaagccat tcccatcaag atacctgaag aaccaatcat ttgtcctact
    52381 ttgaaacgag ccggcgtcca gttatgcaac acaaactcat attgcgacat atcccctgca
    52441 cctttaagat tcactaaatc agtggcccaa gccaaccata acggatcttg cccaaatact
    52501 tgtgtccccg cctgtgcgcc tgataaaatc tcatacgatc cgccaagctg tgtatagttg
    52561 ataggaatcg tcagcatatg atgcaatcca aatggcaata gtaaacgttc taatgttcca
    52621 aataaaaacg gtgctagaat cggtgcgctt tcttgtgatt ctgcaatcca taaaccaaaa
    52681 ttattgattc cagcctgtac gtttggccac accacagcaa gaatcaatgc cacgattgtt
    52741 gaccataaga taacaacaaa cggaacaaag cgctttccat tgaaaaatga caaagcatcc
    52801 ggcaattttc tataattata gtacttatta taagccattg ccccgacaaa tccagcgatg
    52861 atccctacga atactcccat attcaaagct ggtgcttcca atacacttgt aaaaaatcct
    52921 ttaaccatga tcttcgtacc gaacagagtg tgggtaaatg catcttcatt cgcaagcatg
    52981 tcacttgtga caccaaagat agccccagtg atccgattga ttaaaataaa tgagatccca
    53041 gctgcaaatg ctccgcctgc tctttctttt gcccaacttc ctcctattgc taaagcaaat
    53101 aaaatatgaa gattaccaat aattgcccaa ccaatatttt caattactcc gcccgttgtg
    53161 acaagcggcg tccaatcagg attgatcaag ggaattgatt tccctatact gatcatcaac
    53221 ccagctgccg gcataactgc taccacaacc attaaagcct ttccaaattt ttgccaaaac
    53281 tcaaaactaa atatcttttt cattccgttt agctccttta cgcaatcggt ttcgttaaac
    53341 gtattatagc gtgcgttttc ttttttgtaa atgctttatt ttttattttt ctgtctatac
    53401 cattctataa aacctataat acgtgtgttc ccttctataa ttttctccta aaaatttatt
    53461 tttttaagct tttttctatg aaatattttc ttttgtctat tgacttgtgc aatcggttgc
    53521 gtttattatg aacgtataaa acagctcatt aggaggagaa aaatgaaaca gatcaaacga
    53581 ctatttgcta ttgatccatg gaaaatcagc acgacacaat tagataaaaa aaatcttcga
    53641 ctgcaagaat ctttaacaag tattggaaac ggttatatgg ggatgcgcgg gaactttgaa
    53701 gaaacttact caggggacca ccatcaaggt acttatttgg cgggtgtctg gtatcccgat
    53761 aagacacgtg tcggctggtg gaaaaatggt tatccagaat actttggaaa agtgatcaac
    53821 gccttgaact ttatcgcgat agatatttcg atcaacgatc agctgatcga cttggcttct
    53881 ttggaaccag aagactttta ctgggaatta gatatgaaaa atggtgtatt atctcgtaca
    53941 ttcacaatca ctacttctac taataaagta cgcatttcct tcgaacgttt tctgagcatt
    54001 gtcaaaaaag aatcagctta tatctattta actatagaaa tgcttgaagg tagcggaaca
    54061 gtcaaactcg tatcaaaatt ggatggaaat gttcaaaacg aagacagcaa ctatgatgaa
    54121 catttttgga atgagattga tcgtggaact atagaagaaa tcggctattt gacgactaaa
    54181 actattccaa acaactttgg gattgaacaa tttactgtta ctgcagcaat gcgccatttc
    54241 gttaatggaa aatcagtcat ccctgactat cacgaagata ctttagcatt acatggttgc
    54301 ttgtctgcag aattatctca aggtgagaca tggtcagtgg aaaaagaaat tatcgttctt
    54361 actagtagag atattccaga agcgcagcaa gcagaaaaag ctgtctcaga attaacgaac
    54421 cagacaaaca attatatcca agcaaaagaa gagcaaacag cggcttgggc agaacgatgg
    54481 aagcttgccg atgtggtcat cgaaggagac gaagaagcac aacaaggtat tcgattcaat
    54541 ttgttccaac tattctctac ttactacggc gaagatgaac gattgaatat cggccccaaa
    54601 ggattcacag gagaaaaata cggcggagcc acttactggg atacagaagc gtatgctgtc
    54661 cctctttatc ttgctttagc caaacctgaa gtaactaaga atctgctgaa atatcggcat
    54721 aatcaactgc cgcaagcaat ccacaacgct cagcaacaag gattaaaagg cgcgctttat
    54781 cctatggtca ccttcactgg tgtcgaatgc cataacgagt gggaaatcac attcgaggag
    54841 attcaccgaa atggcgcgat tgcctatgca atctacaatt atacgaatta caccggcgat
    54901 acggaatatt tgaaaaatga aggactggaa gttttagcag aaatcgcacg cttctgggca
    54961 gaccgtgtcc atttttcaaa acgagcaaat aaatacatga tccacggtgt gaccggccca
    55021 aacgaatatg agaacaatat caataacaat tggtatacaa atacgatcgc agcttgggtc
    55081 ttgcgttaca cgcgagaaaa ttatttaact tatcaaaaag aagctgctat cactatcagc
    55141 gaagaagaac tagccaaatg gaaagatatc gcagagaaca tgtacttccc agttgacgaa
    55201 gaattaggaa tctttgtcca gcatgacaca ttcttagata aagatctcat gcctgtatcc
    55261 gatattccca caagcgatct tcctctgaat cagcattggt cttgggacaa aatccttaga
    55321 tcttgcttca tcaagcaggc agatgtacta caggggattt atttcttcaa tgatactttt
    55381 acaaaagaag aaaagcaaaa aaactttgat ttctatgaac caatgactgt ccatgagtct
    55441 tccctttcgc caagtatcca ttcgatttta gcagcagaat taggaatgga aagcaaagct
    55501 gtagaaatgt accagcgaac agctcgcttg gatttggata attacaataa cgacacagaa
    55561 gacggcttgc acatcacttc catgactggt agctggttgt cgattgtcca aggatttgct
    55621 caaatgaaaa ctgcccatga tcagttgaga ttcgcaccat ttctgccaaa aacttggaaa
    55681 gcttattctt tccatatcaa ttatcgcgga cgtttattgt tcatctcagt cacagatgaa
    55741 caagttactt tcgagttatt agaaggttct cctctagaaa tttacgtcta tgaacagtct
    55801 tattctttag cggatcgtct agaaataacg ctaagaaagg aggaagcaaa tgtttaaagg
    55861 ggtattgttt gatttagacg gtgtcattac cgatactgct gaattccatt atcgtgcttg
    55921 gcgaaagctt ggtcaggaaa tcggcattac gatcgaccac acattcaacg aacagctaaa
    55981 aggcgtcagc cgagaagact cactctcttt gcttttagct tatggcggaa aagaacactc
    56041 tttttcaaag gaagaatttg cagaattagc gaaaagaaaa aacgattatt atttagagat
    56101 gatccagacg atcgaaccaa aagatgtctt ccccggggtc gtgcctttac tggattcttt
    56161 aaaagaagaa aaaatcaaga ttgctcttgc ttctgcaagt aaaaacgggc cattcctact
    56221 tgaaaaaatg ggtcttacac cttactttga tgctattgcc gacccggcag aagcaacaaa
    56281 cggaaaacct gcgccagata tcttcctcct tgcagcaaaa gctgtcggac taaaagcaga
    56341 agaatgtatc gggatcgaag acgcgcaagc cggtatccaa gccatcctct caagtggcgc
    56401 tcaaccaatt ggcgtgggac gtgctgaaga ccttggagag gagatcccgc ttgtccctga
    56461 tacgactttt cttacgatcg attacttgaa agaaaagtgg catgaccatg gataatccag
    56521 agatctccgt cgtaccagtt ccagagactg aaataaagaa aatcacattg aagaacgact
    56581 acttagaagt cgttcttctc aattacggtg cccgcctgca ccaaatcttc gcaccagaca
    56641 aagaaggaaa atcagaaaat gttttattgt cttacgatcg tttcgaagat gttctaacag
    56701 acaaaagctt ttttggagca actgtcggac ctgtagccgg aaggatccgg gatgcttctt
    56761 ggggtgagca tagattggag aaaaactgcg gatcacacca tatccacggt ggcacgaaag
    56821 gctggtcttt ccaattttgg gaagtagaag ttttcaaaac tccatcatca ataggtgtgg
    56881 tcttttactt aaaagatgaa ttttctacct atccaggacc aatcactgct acgatcactt
    56941 accgtttaac aaaaaacgaa ttagagatga caacctctgc tagtagcctt gttgaaacca
    57001 tctgcaatcc gaccaaccat gcctacttca acctttcagg aaatggaaca agagacatct
    57061 atgaacatca gttaacggtc tttttagatg gtgtgcttga attagatcaa gaaaaactgc
    57121 ctactggaaa ttggacaaaa aaagaaaaac tgccaatcga ctttagaaaa tccccaacac
    57181 tgcaagagat ccttgcatgt tatccagatg gactagatga tgtatttctt ttgcatcatc
    57241 ctaggctttc caaaacttcc ttacaattat tcgagaagca ttctggtcgc caaatgacta
    57301 tagcaacttc taataaaagt atggtattat tttctacgac tggatttgaa gctgatttct
    57361 ctgtcaatgg aaaacagatg cattcaaact acggattagc aatcgaaccg caagaaatcc
    57421 ctgatatcgt tcatttcccc aaatttggat cgatcaacct ccatcctggt caagaacgga
    57481 tcagtcagac gatttatcgg ttctctgcac aataacaaga gaaaggtgtt tttatcatgg
    57541 ctattaccgt aaaagatgtg gcgaaaaaag ccggtgttgc cacctcgacc gtctctcgtg
    57601 tcatcaacga tcatcctagt atttccgaga caacaaagaa gaaagtccga aaaattatgg
    57661 atgaactagg ttatgttcct aacatgactg cccgcaatct aggaaaacgc atttccagtg
    57721 ccatcggcgt aatcttaccc cctcttgatt caaaagagcg gatcggtaac ccattttatc
    57781 ttgagatcat ggaagcaatc aacgaagaag cgcggaatta tgatatgact acagcaatcg
    57841 ctactgctaa aaattttgaa ttgctgttag agaacgtcca gcgtatgcat ttacaaaaac
    57901 aagtagatgg ttttattcta gtgtattcag atagtcaaga tactgtcatt gattatttat
    57961 accaaaacca catccctttc actttgatcg gacagcctta tcatcatgag aacgaaatcg
    58021 tttatgtaga taatgataac cagcttctag gtaaacaagc aacagaattt ttgatccaga
    58081 atggacataa gaatatcctg tttgtgacaa acacgactca tgaaagtttg tatttcgaac
    58141 gatatttcgg ttatcaaaaa gcaatgatga tggctggtct tcctgcgtta tccagtgtag
    58201 tactagagca tccggaggat tatctgtcct ttgagaaaac gttgaaagaa aacgaagcca
    58261 cagcattggt ggtcattgac gatatcttcg cccttcgcac gatgcagttg gcgcaaatgt
    58321 atggctatgt cattccagag acactctccg tcattagctt caacaactcg atcttctcaa
    58381 cattaacaca cccttacctt acaagtatcg atatcgatat ctccgaatta ggacgtgtcg
    58441 ctatgcaaaa gctgcgcgaa ttgatccatg aagaattgag cagtggcgta cgtttggtca
    58501 tccctcatcg cttgatcaaa agagagacag tcgttccttt agaaaaccca tactaaatag
    58561 ctgactcact aaatacgctg accatttatc ctatggtcag ctttttatgt aaaagaagat
    58621 actacttcta acacattgtt tatctgtaaa aaagagcgtg ggacaaaagt aaagaacact
    58681 tttgtctcac gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata
    58741 agctgaaatt cacaaaaatt taaaaagcaa ttttcttgag tttcttctta tttcttggag
    58801 ttaaacgctt ctgtcccagc ctcttttttc cttccttcaa ttttttacac gtcgacgatt
    58861 caataaaaaa ccgcctaata ttgtaaagac aggtgcaaga atcgagaaaa aggcgaatgg
    58921 caagtaatgc aacgcgctaa ctttcatcgt tcccataatg aatgtgccgc tgactcccca
    58981 cggtacgaga gaattgacgg tcgcacctga atccgctagt gttcgtgtca aataggtttt
    59041 tgataagcca acgtcatcaa aagaacgctt gaatgtttct cctggaagaa tgatggacag
    59101 atactgttct ccaacgatca gattgattcc gatacagctc aaggcagtaa acccgactaa
    59161 acgagaaggt cggtccatct tttcttttaa ttcttgaacg atggtatcaa cgatcagata
    59221 tttgatcagt aacccaccta atgccaatgc aagtaaaatc aaactagcag aacctaacat
    59281 actcatgatc ccgcctcgag aaagcagttc atctagcttt ggatcaggtg tatttgcgac
    59341 ataaccattc atcaagatgt ctgctgcttt tccgaccgtc aaacttttct catggataaa
    59401 agaaagaacg agagcagctg tcgatcccgc tagtaatgta gggatagccg gaattttttt
    59461 gattgccccc ccaaataaaa taactagtgg aatcaatgtc caaggagaga tccaaaagct
    59521 cgctttcatg ttttggatca tttcattgat actagctaga tcggctgaat gactgttatg
    59581 tcctaatatc gtataaaaaa tcaacgaaag gacgaatgct gggaaatccg tataacgcat
    59641 accagcgata tgttcgaata gattaactcc tccaataccc gctgccaagt tggtcgtatc
    59701 tgataaaggc gagcagttat tccctaaaaa ggcaccggaa acgactgcgc ctgtagtcat
    59761 tgcatcattg aatccaagta aatgaccgat ccccaaaaaa gcaattccaa tcgtcgaaat
    59821 cgttgtaaat gaactgccga cagtcactcc gacaattgca caaacaagaa atgctgtcgg
    59881 taaaaagaaa cgcacagaaa caagtttaaa tccataaacc ataatcgtag ggatggttcc
    59941 agataaaatc catgaagcaa ctaagacacc aatcaaaaga aaaatcagga ttggaatgat
    60001 ccctggagtg attcctttga tgatcccgtc atggatctca tcccatgtga actttttaaa
    60061 tctgccataa aacaacaaac acatgaaagc tgcaaggatc gggatatgtg gcgagagctg
    60121 gaatccgatg atcagtactc ctaggagtgc caacaacacg atcaatacag cgatactttc
    60181 ttttaaagaa atatttttat tcatcgtcga tctcctattt caaatgaaaa ggccctgcca
    60241 tgaaaaaact attcacttgg caggacctcc tactaaatca gcgacaaaac gtcggctgaa
    60301 agacttattt ttttattttt cttctaaact tttttctacc tcaatccccc aagaatcttt
    60361 cttgaagtga ttgatgatca atggaataac gaacatgacc actgtgatag ctgctactaa
    60421 taatgcatca ctcaatgtcg ctgtacctaa tgtagcaaat acgacaacga tgatagagaa
    60481 gatcagcatg cagctgacta cccaagctaa tccgtttccg tttttaccaa ttcggtaagg
    60541 acgttcgaat tctggatgtt tcttacgcat tttaagcaat gaaacagcaa tcaaaatgta
    60601 cacgatacaa taaataacag ttgtagcatt tgtaagtgtc aagaatacag cacttacgtc
    60661 atccattaca ccataaagca aagcaaataa tgaaataacg attgattgtg ttagaacgac
    60721 atttctagat acgccgtatt tgttttcttt atggaatccg aattttggtg gaaggaatcc
    60781 ttcacgagct acttggatga ttgtttttga tggtccagta acccaagctg ataattgtaa
    60841 aagcacacca ataaatacca tgaagctgaa gatatttccg atcacttctg gtaagcctag
    60901 gatttgacag tcaatcaaga taggctgcgt gatatttgct aattccattt ttccatctgg
    60961 tacgacattt gctacaagca tcgcattgat gacattcaat aaaactaatc cgatcaaagc
    61021 gataaatacg ccttttgtat aattttttgt tgcgtctttc aaacgaggca tgtaaacaga
    61081 actcatttcg atcccaacaa aaataaatgt gataccagct aagtatttca atgaatcaat
    61141 atgttccaag tcagggaaag cttttgacca tgaaaagtca cctagatagc cgtttgagac
    61201 tagtccgact ttgaaggctg ccaaaacacc taaaacgaac atgatgacta ctgggatata
    61261 tacccctagc catacaccga tgttcccgcc gacttttgcc atatcgaatt tcaagttcaa
    61321 gatcgtgatg atccagtaga taactaaaat acaacctaaa acgaaccagt gattattgcc
    61381 aagttctaca ttaccaaatg tgtttcctaa caatggtcct aaagtagaag cgaccattac
    61441 catacctggg aacatttgca cccataatag ccatgcaacg acaaatcccc atttgctgcc
    61501 cagtgctgtt tttacccata actgaggacc gccttcttga ggaagcatcg tggaaagctc
    61561 accggaaacc attgatatcg gaccggcaaa aaacaaaact gcaaaaattg aataaaaaat
    61621 caatgtccaa ccgactgcgg ctaacgtagg aatactccga accgttgcac acagtgccat
    61681 tgtcagaccg ataaaggtcc caagacccat tttacttact tcagatttac tcaccgttga
    61741 cactctcctt gattttttgg ataaaataac ggtgtctttg aagtagtttc agcaataaac
    61801 gaaacaattc agcgaatatt tgaggggatt ctttgagttt attgcttgaa gggtttcttc
    61861 aaagacaacc tcaaaacaaa gggatcgaag agcttaggtc tccccttgct cttcagatcc
    61921 aaacatgcgt cagaaacagt tagtattcaa gctgttttct aacatgatta ccttgcttat
    61981 tttgcttcgc ttgccaataa ttggtctgcg tagatttttt ccaatttttc ttgaagagct
    62041 gctttgattt tttcagcata ttcttcaaag tgttcttctt tgttcatgta tggtgtcatt
    62101 acgctggcac gcaatacagt tacttttccg gcacggttcc attcttcatc tgagaatcct
    62161 agttggttca caaattgtaa tgggctgttg ccatagtctg ggatagcaaa gtcagtatgt
    62221 gaagttaaga actcattacc gtaaatgctt cctttcacgt atgaagaata atcatatacg
    62281 tcatggttca atttattcat tgcaactaag tcatcgttgc ctttttcttt gaatacatag
    62341 tctaccatat tgaaatctgg ataagttaat ggatgaactt cgatttcttt gtctcctact
    62401 ttaaatgaca agtcattcaa gaagttgtag aaacgatgtg caccttcgat tgatgcaccc
    62461 attaatttac catatcctgt gacattcaaa ggtaagacat ggtgtgctgc ccaaacgctg
    62521 gctgctgttg cccctgcttt tgaaccttcc aagatataag ccccaagtaa tgctgggata
    62581 tctgcgcctt tttcaaatac atatgtcgca aagtaagaga taacgtctct catgcggata
    62641 tctttgatga cgataccacc ggcagaatat ggaacatagc ccattttatg tgggtcaatc
    62701 gttacagatt ctgcttcttc gattgcttta tatgcactgt gtacttcttc taagatataa
    62761 tctttgtttt ctgtaaagac attgtatttg taatgaacat cttttaaatc ttcaaatgga
    62821 atgaagttgt tgtcttcgtc taagaaaatc gcacggccgt atccgccata agcagcatct
    62881 acgtgtaagt agaagtaaat accgtctttt tctaatacgc gacgcaatgc aacgatttta
    62941 tcgataccgt cgattgctcc ttcttctgtt gatcctacaa caccgactac acctaggatt
    63001 ggtgtttttt cagctgctag accacgaacg attttttcaa gttcgttgat gtccatgcga
    63061 tagttgtggt caactggcac tggaatcact tggtctaaac cgataccgat gatgtcggca
    63121 gcttttagcc atgaatagtg tttagtttgt ggaaccaacc attttcctaa tttttctaaa
    63181 tgttttccgc tacgtgctga gtgagctttg atttcgtcga ttttttctgg gacgctgtct
    63241 aacagattca tgatttcttc tgttgacaag ttcattaatt cccaatcgct ttttccagca
    63301 actagttcag gtgtcacttc tttcattgca agcggcaatg atttgatgtt gcgggcatac
    63361 caaagtcctt ctaagtttgc taaagaacca tcagcaacga tgtggcccca gccatcttta
    63421 tagctcatta attttgcaaa ttccattcct acttcttctt ccatttggct tgttgcagga
    63481 gaagattcat aggcaacgtt attaccattc catagcatag caaaattgta cgctagtaaa
    63541 gatggcatca atgtttcaga gttcatatga ccccagtaac gtccggcatt atgccaagga
    63601 acggaatggg tacgcatccg cgctgaaatt tctgatagaa catcctttgt tttattcaca
    63661 gtgtgttcaa aactagcact gcttttttct tctggcgtaa taataggcat gtcttgaggc
    63721 atgtagtttt gacgccagcc taaatgttca tccaccaatt cattcaacaa agctttatag
    63781 atttggccgt tctcagcttt gtccccaatg aatagggcat ttaggtttag atctttcgac
    63841 aatgattcac tcatagtgaa accctcctaa aaaaatattt aaattttgtc gagccctaca
    63901 ttacacctgt tcttctattc tgtaaactaa gttttggcct gatttttaat catattttaa
    63961 tatgtatcaa ttatccgaat tgttcacact tttgaaataa ttaaaaaaaa cgagggaatt
    64021 tacaaatgta aactccctcg caaagattgt attaacgaca accagcgttc tattttagga
    64081 tgggtttcaa aattggaatg ttgtaatagt atttcccttt ttcaaggtaa aaacttacta
    64141 aattctaatc aattactttg gccaaaaagt aatttttctt accttttcga ataaccacaa
    64201 acttaccatc gaattcatcg gatggattta tgacaaaatt taaatcggta acgcggtcac
    64261 cgttaatact tatagctccg tttgaaacat cttcacgtgc ttgacgttta gatggttcaa
    64321 tttttgtgga aacaagcagt tccacgatat tttcaggtgt gcttgaaatt tcaacgttcg
    64381 gcattttacc gaatccttgc gcgatttctt ccgcatttag ttctttaata tttccagaga
    64441 acaatgcttg cgttattttt tgcgcttctt tcaagtcttc ttcactatgc acaaatcttg
    64501 taacttcttc tgccaaacgg cgttgtgctt cacgtttctc aggttctgtc tctacttttt
    64561 tcgctaagtc ttcgatttct tcttgagaca agaaagtaaa gaatttcaag tatttcacta
    64621 catcgcgatc atcttgattc aaccagaatt ggtagaactc aaatggtgaa gttttcttcg
    64681 gatcaagcca aacggcaccg cctgcagttt ttccgaattt cgttccgtca gccttcagca
    64741 tcaatgggat cgttaaaccg aacgcttctg cttctggtcc ttctaatttt cggattaggt
    64801 ctaatccagc tgtgatattt ccccattggt ctgctccacc gatttgcaat tgcacatcgt
    64861 atgttttatg cagatgcaaa aagtcgatag attgcaggat ttggtaagta aactctgtaa
    64921 atgagattcc tacttccaaa cggctggcaa cgatatcttt tgctaacatc gtgttgatgt
    64981 tgaagttttt accgtagtct ctcaaaaatt caagtaaaga gatttttgac aaccagtcgt
    65041 agttgttcac aaatgtgata tttgcatctt taccaaacaa ttttctcatt tgattggaaa
    65101 gtgcgtccac gttatgttgc acttgttcca tcgtttgcaa cacacgttct gttttgcggc
    65161 cgcttgggtc gccgatcgtt cctgttccgc cgccaatcag aatatatgga tgatggcctg
    65221 ccaattggaa tcttttcatc atcataaatg gaatcaaatg tccgatatgc atcgaatcac
    65281 cagttggatc aacgccgcaa taaagtgaaa tgctcttttc ttcaacgagc tcgcgaagtc
    65341 tttcttcgtt tgtttgctga ttaatggcgt cgcgccaagt taattcatca ataatattca
    65401 ttttactgac ctccgtgtag tttttttatg aaagcaggaa cctgctagca tgtgtaatta
    65461 attatttctt caggatagga taggaaataa aaaaagtccc ttggcatata agccaaggga
    65521 cgaaatctcg cggtaccacc caatttgtat gtctagatat ccaacatacc tctcgaacat
    65581 ttaacgctgt cgcgtggcaa tcactgccaa agactcctga aatgtaattc gctttaaagc
    65641 atgtactggt ttgcagcgac caccagcttt ctgaaacatt ggctttaaca actacttcgt
    65701 ttcgatcttc gtcttgttat tattaatatg tgtctatcat agagcgcaac tttttctttg
    65761 tcaacagaaa aaggatattt tttttaaatt cctcattttt tcttttttga atccgctcta
    65821 attccgaaca ttttattttc aattattgtt aaaagaaata actttttctg tattttttct
    65881 gcaatttatc gagaagtcac tttactttgc ctcttattct ttcttcgtta caataaggaa
    65941 tgggacaagt aaaaaaatga aaaagcaagg gacgaggtat atggaaaaaa agcaaaaaat
    66001 tattttagga gccacttctg ctttaactat cgttatttta attctaagtt tgttttttgt
    66061 catccgatca tctacaaaaa aagaaccaat cattcctgaa aaaaaaataa cagaaaaagc
    66121 aaaagaaccg acagatgata agcaacaact agttactcaa gcaaaaaatc tggctcaaag
    66181 ctacgattat gaccaagcga ttgcgttatt ggaaaaagat agtaagaacg gatctactga
    66241 aacacaacag cttcttcaaa cactaaaaaa ggagaaagaa caactgatcg actggtctga
    66301 tcctactcag atctctcatg tctttttcca cagcttgatc gtggacccgc aaaaagcctt
    66361 ccactctcaa caggcacaag gatacaaaga ttatatggtg actgttgaag aattcaaccg
    66421 ttcgatcgaa caactttatc aaaatgattt tgttttagtt aatttgaatg atttgatcca
    66481 aaaagacgaa caaggaaatc tttcctttaa cgggcttgcg ctacctgaag gaaaaaaacc
    66541 attggttctt tcacaagatg atgtcagcta ttatgaatat atggataatt ccggttttcc
    66601 tagccgattg gtcgtatcaa ataacaatga aatcaaaaat gtctataaag agaaagataa
    66661 agaagaaatc ggtgactttg acatggttcc tctgatcgat gcctttgtca agaaacatcc
    66721 tgatttttct tatcgaggcg caaaaggtac cttggccctg acaggttaca acggagtttt
    66781 aggttatcga acatcaaaat ctcaatatgg agacaatgaa aaaacaaaaa aagagattaa
    66841 agaagctaaa aaagtggcgg aacagttgaa gaaagacggc tggagctttg cttcccatac
    66901 atggggtcac ttgaatatga ctcaagcttc actagaagat gtaaaaaaag acaatgaatt
    66961 gtggcagcaa gaagttgcac ctatcctagg aaaaacaaat attctgattt atccatttgg
    67021 tgcagacatc agcgactggc agccttattc agcagaaaat caaaaatttg cttacttgaa
    67081 gagtcaaggt ttcgatattt tctgtaatgt cgatgcttct acaccagctt ggggacaatt
    67141 aggtacggat ttctatcgaa atgctcggat caatatcgat ggcattcgtt ttcaatctga
    67201 tttagacgga aaaaatccta ttcttgatcc attcatcaat gtccatgatg tctatgacaa
    67261 agaagcacgc gaatctgctt gattttacgt catttatgaa ccaaaaaagc cgaactgaca
    67321 ttttatcgtc agtttcggct tttttatgct ttatttcttc ttcggtgcga ttcccttgat
    67381 caggacttct gtccattctg ctgtatatgg aagcagtttt gcgtatatct ccggcttaga
    67441 aattccttct accattgctt gaaaatagag gatcaagaac tcatccgaat atttcaaatc
    67501 cacttttcct tctttccgcc ctcgatggaa aagatcaagc atgatactga aactttcttg
    67561 cgtatacgca tccatcgcgt cagaaaattc ttttttttct ttttttgtat agtatgtcat
    67621 caaatcgata taaaattgtt tgttcactcg attgagataa cggattttgt tttcactcat
    67681 ggcaatcagt gtttcttcaa atggaagatt ttccgcaatc aacgctttcg cttcttgccc
    67741 taaatcattc ataaatgcaa aaaatacttc ataaatcagc ccttccttgc tgccaaaata
    67801 tttaaaaatc gtcgctttac ttacctgagc gttctcagct accttatcca ttgtcactcc
    67861 agcggttcct atgtctgtgt tcatcaagtc aaaagctgct tgtaaaatct ctttcttttt
    67921 ctcttgtgct ctcttttcaa aaccgttcat ttgtagagcc tcccgtaatt ttaaactttt
    67981 ataatatata tagttcaaat ttattctgag gtttattata actatctatt gagaaagtat
    68041 caagctagca attccttgtt tatgaattca ttgacagaaa tttataaaaa gaatattatg
    68101 tactaaaata ataaaaaaag ttttataaaa aggtataaaa cctttttatt aaactattta
    68161 atcttgttta gtatatattt ttagaaaaag gagtggggtt tttatggcag aaatcgtcaa
    68221 agtcacacat ttgaaaaaga gctttggtcg tttcgaagct ttaaaagatg tctctttcac
    68281 cgtacattca ggtgaagtcg ttggatttat cggaccaaat ggttctggaa aatccacgac
    68341 gattcggaca cttttaggga ttataaaaaa agacggcgga acggcaacga tttttgggca
    68401 agatgtatgg gaagatagtt tatctatcca tgaacgtctt tcttatgttc caggcgatat
    68461 ctctttgtgg ggaaatttga ctggaggagc gatcatcgat ctcttcatga agcttcatgg
    68521 aaaagggagt gctcaaaaaa aagatgaact gattcaacgt ttctctcttg atcctaagaa
    68581 aaaagcaaaa aactattcaa aaggaaatcg ccaaaaagtg ggcttgattg ctgctttggc
    68641 tgttgattcg gatctctatt tatttgatga gcctacttct ggtttagatc ctttgatgga
    68701 aacgattttc caagaagaag ttgaaaaatt gaaagaacag ggcaaatcca tcctactttc
    68761 ttctcatatt ctcagtgaag tcgagcgact agcggataaa gtcgtgatca tacgcgaagg
    68821 aacactcgat gaagaaggaa cactcgatga attgcgtcat ttgactcgtt cgaccatcac
    68881 tatagaaaca gaagcagaga atagtcactt agcacaattg aacggtgtcc atgactttgt
    68941 gcaaaaaggt cacctcgctc aatttgctgc tgataacgaa gctttcgatg tgattttagc
    69001 tgaagcgtcc aaattaggtg tgaaaaaatt tgaatctatt ccaccgactc ttgaggattt
    69061 attcatgaga cattatgaag gatgaggagg aagcgaaatg actgataaat tttcacgctg
    69121 gtccaccctt ttttctcaaa accttagccg tgactggaaa aagatcctga tatggctagt
    69181 cggtgtcact gctttttctg gtggttttgt ccctgcgttt gaagaattga cttccggaca
    69241 aggattagct ggattatacg aaaccttgca aaacccggca atgattgcaa tggtcggacc
    69301 aactccagct tcgaatgctg ctgagtatac tttaggagcg atgtatgcaa atgaaatgtt
    69361 gcttttttgc agtttgtttg ctgctgtgat ctctgtttta cacgtggtga gccaaacgag
    69421 aaaaaaagaa gaacttggtc tgattgaact tattcgttcc tttcgagtcg gacgtcaagc
    69481 aaactcttta gctgtcatcg ctgaaagtat tctgatcaat ttgcttttag caatatcgat
    69541 cagttgtacg atgatttttt tccaagcgga ttcgatcact gcagaaggtg cttggctttt
    69601 tgggctatct gtaggtagta tggggatttt ggggagtgcc gttgcactgt ttgctgcaca
    69661 actgatgcca agttcagcca gttcgaatgg tctgtcttta gcgtttttag gtggacttta
    69721 ccttctacga gcaataacag atgtagctga ttcctccctt tcgatgtgga aacctttagg
    69781 ctggtcttat ttgacctatc catttacaac taacaactgg attcctttgc tttatttgtt
    69841 tttgttcagt ctaatcttga taatcagttc atttgtttta gaggaacatc gagatttgaa
    69901 caccggctat atccccgaac gtgaaggaaa accgtatgct aaacgttctc ttttatcctt
    69961 gcgtggttta ttattctatc taaataaagg gatgacaatc agttggctta tcacttttgg
    70021 tttgatgggg gcggcatacg gctctatcta cgggaatatg caacagtttt tagaaagtaa
    70081 cgacttaatg aaacagatgt ttatccaatc tggcgtctca attaaaaaaa ctttacaagc
    70141 acgatcatgg tcgtcttgat ttgtctagct gctattttac caattgctag catcaatcgt
    70201 ttatttacag aagaaaccca cggacgtatg aatcagctgt tcgcaacaaa aatttctagg
    70261 aaccaacttt attggaccaa tatgttacta gctattctat ttgctatatt ggcaatcctg
    70321 attagttctg gtgcactagg gctttctgct ttggcttcga tgaaggatgc gaaagacatg
    70381 gatctattga tgtttcttaa agccgggttc aatttcctgc ctgccgtttt agtgtttatc
    70441 ggaattagtg gctttctatt cggatggctt cctcgaatcg gtaaacttgc ttatatctac
    70501 ttagcttact cctttgcact caattacttt ggcggaattt tggatttacc agaatggttt
    70561 tccaaaacag ctgtccctag ctgggttcca cggatgccaa acgattcttt tgatctttcc
    70621 gtgttcctta cgttatcatg tatcagcctt gcactgttcg tccttggttc tattggttat
    70681 aggcgaagag acttactaga aggataataa aaaagcatgt acaatttcat gctagcctcc
    70741 aaatctgggt caaaaatcca acatttggag gtcatttttt tatttacaag tcttctaact
    70801 gctctttatc aatgatccag acttctcgat tctttctttt tacataacca ttctcctcta
    70861 ataatttaaa ttttctagat aaagtttcgg gggtcgtacc gataaatgca gcaagttctt
    70921 tcattttcat tggcaggaaa aagtgatcct tccctgtcgc tttagctaag tcaagcagat
    70981 agacagcgac tcgttcttct actttttcca tcatcaaaaa agttgtctgc tgctgtactt
    71041 ttgttaattt ctcaacgttg attgctaaaa gtttcagact aagggcggga taatgcagta
    71101 agagttccga gaaatctttt tgtctcaata tgcaaatttc cgttttttct agtgcttcgc
    71161 caaataaaga ctcatttgga acgcctagca actggctctc tccctcatat ccgcccggct
    71221 ctattactcg taaaagctgt tcctttccgc tagcagataa ttgatacact ttcatactgc
    71281 cggaggccac gatcactaat tgaggttctc cattcggcgc aagaatctgt tctccttttt
    71341 ccacatagtg atgtttgaca agtgcgtgta ctttttcttg atcttgctgt actaagtgat
    71401 tgaacaaagg aacaaatgaa acacataaat gatcatgact cataatctct cctccttcta
    71461 aacagctatt tctaataaag aaacataact tcccttattc caactgataa ttttctttta
    71521 tcatagcata aacgtcaagc gaaccagata ccagcaaata gagtggattt ctttttgtcc
    71581 cttgctttat tttccttctt ttgagacctt aagtaattga ttcctatcaa ttttttaaat
    71641 atttactttt tgaataatca aaaacaaacc ttcactcgca agccgagagt aaggtttgtt
    71701 tttcgcattg cttatttcgt gttgtctatt gaaatttttc cgcacctagc ttttgatata
    71761 gtggtgctag ttcttgtaag gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg
    71821 gatagaagaa tagtttaccc atttgaaagg ttcggtctct catcgctttt ttcaactctt
    71881 cttgtttctt tgtatcggac aaatagtcag acaaagctaa gtggtcgtca agttcatgtt
    71941 ctggttgcgg ccatatgtaa taatccttga tatcgactgt tttgctccac tctttcagta
    72001 tcgcaagcaa ctggttatac gctgcaatgc taccatatcg ttcttcgctt ttataatgtt
    72061 ggtacatcaa atagatttgg tattgttttt gattcaacaa aactgctaga caggcattct
    72121 cgtcttgacg agattcgctt cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat
    72181 tcgtccagct ttcgatttta ggtttgctca tgccgtacat atcaggcaaa agtgcagctg
    72241 tctggagctg aatctctttc catttttgcc agtgttcttt atattctttc ttgatttgtt
    72301 caatcttatc tgttggatgg ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa
    72361 acaactgata atctgatttt ccaagttcca ttgtttcgcc tctttccgtt atcttttgta
    72421 ttctacctga attttgcgat atggacaaat agaggttgtg aaaaagctgt tctgcatcaa
    72481 gtcataagaa cagccaaaca aaaaactgcg atgagcatca ccgcagtttt attgaactta
    72541 aggttgattg atcacatcta aaagcgttga aaataaaatg gcactagaag cagctcctgg
    72601 gtcgatatgt ccgattgcac gctctcctag ataggaagct cgtccttttt tagctaccaa
    72661 gactttggtg tgttcttttg cctggtcaat gatttctttt gttaaattcc ctgccttcaa
    72721 tgcctctgtg actggcagcc aaacatctaa catcgtttta tcgtcagctt ctgcctttcc
    72781 tcggctttga atacccgctg ttccctgctc gatcacttcg cctaattttt cttgagaatc
    72841 aatagaatcg acctcttttg tcgctttcat catgttcatg aatgcagatc cgtataacgg
    72901 accagaagcc ccaccgactt tcgatatcat cgccatagag aatactttga atgtatcgct
    72961 gattgtttca ggatgatcgg cttgaaaagc ttcttcataa gctgaaaccc ctcttcccat
    73021 gttatttcca tggtctccat cgccaatcgg ggtatctaga tcactgagat attgtttgtt
    73081 ctgattgatt gcctctgcaa attgcgtgag ccattcttgg atttcttgaa cggttagttt
    73141 catattatca cccttttatc ttgagatttt ttaccatgaa atcgtctgta cattgctttc
    73201 taaagaatct ttccattgtg tctccgtcaa gtcgatcagt gttagagaaa gaccttgcat
    73261 atctagtgag gtcataaaat taccgacttt tcggaaaact acattgacgc ctctttcttc
    73321 caacagagct aaaacatcat tcatgaacac gaactgttcc atcaaaggcg tcccgcccat
    73381 tccgttgact agtacacctg cttcttttgg taaattcgag taatcatcta aaatcttagt
    73441 aacaagttcc tttgccagta ctttagaagg ctgcatcttt tctcgacgat agcctggctc
    73501 accgtggata ccaacaccat actcgatctc atcttctcct agttcgaatc ctggtttccc
    73561 tacttctggt acagttgctg ctcgaagcgc tagaccgatc gtcttagttg cctgtacgac
    73621 cttttctccc agttctttca actcagctag ggaagcaccg tttcttgctg catcgcccac
    73681 gattttgtgt acaaggacag tacctgcaac tccgcgtttt ccagcagtat acgtactatt
    73741 ttcaacggcg atatcatcat ctacgatcac actttctact tcaatatcgt ccatagcagc
    73801 taattccttt gccatatcga agttcaatgc atcccctgta taattcttaa cgatcagcaa
    73861 aactcctttt ccactatcgg cttccttaat agccgtctga atctgatctg gagttggaga
    73921 agtaaacaca tcaccaagca ccgctgcact taacatccct tcccctacaa acccggcatg
    73981 ggatggttca tgtccgcttc ctccaccaga aaccaaacca acttgttccg ctttctgatt
    74041 cttggctaca acacgggaag attctacacg atgaacaagt tctggatagc tttttactaa
    74101 tccttccagc atttcttcga caatattgct tggatcattc atgatttttt tcaatttatt
    74161 cccatccttt ctttccggca ttttgtgctt ctgttaagat ttgctgagta tcatctgtta
    74221 taccggcagt gatggctgct gcaaaagctc cttccactaa aggcgcatcc accagatgat
    74281 aacgttgctg ctgctcttct tccaacatgt cttttgccag ttccgagttc aataccgcgc
    74341 taccgatatc cgcaaaaaca aaataactag ctgcatcgct tgattcattg actgcttcta
    74401 cgattttcat cgggtctgat ccgatctcgc catttattcc gccgagagaa aaaatttgca
    74461 cctcttcgga tttttgcatc tgctcaatca tttctttaat accatctgtt actttttgac
    74521 tatgagaaat aagcaaaata ctttttttca caagaatctt cctttctttt acatagttgc
    74581 taacgattgg tttcgctttc attaatgaac aattcaagta tataataaat ttctaatttt
    74641 gggaaaatat gtgtagtgct tcttgaagtt tgttatactt taattaaaag agaggagtga
    74701 tcgaatgatt acaacaacaa cgaacagtgt agagggaaaa gccgtagtag cttataaagg
    74761 gatcgttttt ggtgaggtca ttactgggat ccatgcattt aaagacttgg aggcggggtt
    74821 gcgaaatatc tttggcggcc gctcaaaaag ctatgaaaac gaactaatgg gtgcaagaga
    74881 agaagcactt gccgaaatgg ccgaacgtgc aaaagcatta ggagctgatg cgatcattgg
    74941 cgtaaaaatg gactatgaag tattaggcag cgacaatggc atgctgatgg tcacatgtag
    75001 tggaacagct gtctccacga gataagtagg ggacatctgt tttaataact aaagagagat
    75061 gggacatgta gtggcactca agaattagat ttttcagtct aacttcgagg tactctacct
    75121 tcccatcctc tcttttttat ttctttttgc ggatgattcc gtaagctgct gcgactaaca
    75181 cacccgcccc tgctaaggaa taactgacag aagctgtttt ttcatttgtt tttggcaaag
    75241 tctgacttgt tgatttggat ggctctgtcg tttgctgaac tgtttctgtc gtgcttccag
    75301 aggtcactga tttttctcct gccgaatgca aaatatcttt tattcgttgg aaactagctg
    75361 ctaagtcagc tttttttgct tcaaaatcat tagcaactgt ttgtgcttcg ctgatctttc
    75421 cgtggattgc ttgactggcc gctgtgatat ccgcttgagt ttgacttagc tttccagaag
    75481 ctgtttgagc aacctgattt aattgctgat tgaattcttt tgcttgttgg atcttcccgt
    75541 caagatctgt ttggacagct tccgcttttg ctttcgcttc ggcaagcttt ttctcaagtt
    75601 gtgcacggtc atttcccact ttatttttaa gatcagctaa atcttgatcg attgctgctt
    75661 ggtcttcttt caatttgttt tttgcagctt ctaatgcttc ttcgagttct tttcttttat
    75721 tttccaagtc ttgcaaatga tcgatttggt cagaagaggt agaactattt attgatgtat
    75781 cttctgaaga tgaaatcgtt tctcctgaat tatctgtcgg tacattggaa gtcatttctg
    75841 tttccgaggc accgttttca gcaccagaag agcttatggt tgttgaaggt tctgtttgag
    75901 caggttcaga agtttccgca aaagctgtct gtcccaataa gaaaatactg gaaacagcaa
    75961 ttggccagat taacggcttc attctttttt tcatcacttt tcccatcctt actacttttt
    76021 aactaatttc ttattataag caaaaatgtt tggaagaacc ataaacacaa caggctgttt
    76081 gtctaaaaaa cggtgcatct tttatttttt tgtacgttcc gggcttttcc atgtcccgct
    76141 tccataacct aaaatcgttt tgactgctgg aatgaaagtc gtcacgatcg ttatcgcgtt
    76201 cactatccag aacaaaagca tatatagtgg tgcgaaaaga aggtatttca attttcttcg
    76261 tcgatcatct acaatcagcg aagctagcaa ctggaaaaat cctgcgacca tttcaaagca
    76321 gatgaacaca aaactgaatg taaaagtaat ataaatctgt tcgtagtttc cttgatatac
    76381 ataatgcccg attaatccag caaaaagaac tacggaaata caaaagaata ttgaccagac
    76441 aatactcaat gtttgatcaa tgaagatgat cgttcttcca atatgtttaa aaggatggag
    76501 catcactttt ttgaaattcg tcagccagac ttctgttcca ccttttgccc aacgttttct
    76561 gtgtcgatat agcatcttca acgtcacagg aacttccatg aaaaagataa tctcaggagc
    76621 aaaaacagat aaccagtcat ttaactgatg atcccaagca atactgatgt cttctgtcgc
    76681 tcgatcttgt ctgaatccgc ccacatcgat caaggcttct tttcgataca tcgtatttgc
    76741 tccactgtac gcataaagac caccgaaaac agctgactgt gttcgtttaa tgatgcccac
    76801 gatacttgag aattcaaccg tttgagattt tgcaatcaat ttggtacgat tttggacatc
    76861 catatttgca gtgaccgcag caatatgtct tgcaccaggg cgaatgaaat aattcacata
    76921 cctaatcagt gcatctggct ccggtaccgt gtctgcatca ttgcttaaaa tcaatttccc
    76981 ttttgcaaag gcaagtccga tattaaacgc gtgggctttt cctttatttt tttcgatccg
    77041 aaccacacga agattagcgt attttttcat cagtctagct aatatttctg gtgtttggtc
    77101 cgttgaacca tcgtctgtca ctaatacctc atagttgtga taattcaatt tcgtcatcaa
    77161 atactcgatt gtatattcga tcacaatttc ttcattatgg gctggcacca tgatcgtgat
    77221 gaacggttcg acactcaagg gaacatctac ccattctttt tgcttatgtt tgtatataaa
    77281 cacataacac cagacaccaa taaaccatac aaatccgcct aaaatcggat aagaaacgag
    77341 aaaaaaatac ataatagaca aacaagtatc taagaataaa ttacctgtat gggtgattga
    77401 gatcatttca gttccacccc attttcttta tacaaatctg caatgaatgt cgtatctaga
    77461 tttttctctt cggagacaga ataaaaacgg accgtttctc tctcttcttt tgttccaaaa
    77521 cgttccgtat acacttcttc aagtaattct tgtctttttt tcaatttttc atcttcatat
    77581 tcaaaagatt tatttacttt tttgtataga tttcgattgt tccacctagt caatacaaat
    77641 gaggtaatca ggaaactgaa aaaaatcaat acaaaaaagc cgattaagaa atacagcatt
    77701 ttcttttctg ataaaaaagc gtacaaggaa aaatccttgg ctctttctgg tctcgttaat
    77761 gggaaaaaga cccatataaa aggaagaaca acgatcatcc atcctaaaag agccactaac
    77821 gtttgccaaa tctttaacca gtaattacct tttgaaaaat acttatcttt caccaaaatt
    77881 gatgcaactt cttctcttgt tacttttttc atatcctcac ctcacgtttt tttgttacga
    77941 cgtaggagtt taccatacat ttcatttctt ttcacataaa gtgttcctaa atttttccta
    78001 agaaaaagaa caaaacattt ctcatcagca aatgaaaaat attttgttct ttttatcagt
    78061 tgttcttttt attttttatc tcttaattat gttgctcttt ccaatacgac aatgcttttc
    78121 attcctctag atatcgccgt gtacatttgt ctgcgtcttc tttgctcatc tccatcgttt
    78181 tcaaatggat ataagattac atgatcaaat tccagtcctt ttgctagact caaaggaatg
    78241 atttggcagt tttctaatcc atatgaattt tttttaatcg attcttgtag cgtttgggtt
    78301 tcctcttctg tttgggtgat aactgccgct ttttctcctt ttagagaaac caaaatctca
    78361 gctagtattt tcaacaattc ttcagaggat cgaaaagagc ggtattcggg ttcttttcct
    78421 tgcggtcgga tcgatacgat gtcgatcttc tcttgatcca cagcataggt tttgaaaagc
    78481 tctgtgattg ctccgctaga acggtaacta tttctaagat cataccgatg gatcggcaga
    78541 tggtagtcgt cgaaacaacg cataatatca gcaaaagtag tggaagaatt aaagattgcc
    78601 tgattctcgt cacctactaa agtaaagtga gtttttggaa ataattcgat caatagacct
    78661 aattgtgcca gggtataatc ttgtacttca tcaatcaata gatagcgaaa tgctggtaca
    78721 tttacctttt caacaagtaa atgacgaata gctaatataa tgactgcttc atcaacagta
    78781 cgtgtgggtt gccaagcata agctgagcca tatcgttttt cataaattct ttcaaacaac
    78841 tgatgctcat ccacccatgc catttcttcg atttgtctgg ttatttttcg atattttttt
    78901 tgtaaaagtt tccttgcata agctacaata ctttgttccg aatcatcaga aatcaattta
    78961 ccgaataatt ccaactgcct atcttctgtc aacgagagta cctgattctg cacagcagga
    79021 ctttttgctt gtttcaaaag atgattcttc cattcgctca tcagtgcctt tttcgttgct
    79081 tgaagcctat cgatcaattt aggatgagca ggaactttct catatatttt ttcgatcgtt
    79141 tttttagaaa ggactgtttt tccattttta gtaagtccac ggataaaatt cggatgatct
    79201 aaaaacaaag gatctgattg ttttagatta tcgataaact ttttgctacg caaacgttct
    79261 gtctgctccg atacattttc tcccgtgata cgtttgaaat aagcctcttc accttctatt
    79321 tcctctgctg aaagctgtga aaccagctgt atgatggtta gattcagtgg atttctttca
    79381 cctaatgatg gtaggacatt cgaaatatag tcgataaacc gatgattagg tgatagaatc
    79441 agcaagtcat cggatgtcat cgtatctcga tataaataga gcagatacgc aatacgctgc
    79501 atgatagcag aggttttacc acttcctgct acgccattta ccaagatatt ttgacttttc
    79561 acatcccgaa tgattgtgtt ttgttccgat tggatcgtag cagtgatagc cttcatttca
    79621 ttggtctcat cttggcctaa gacttctaat aatacgtcat cttgaatagc tacagacgta
    79681 tcaaaaaact tgaccaaacg atctctttcc aaaagaaatt gtcgtctttc tttaataacc
    79741 gtatcgattt tattttgacg aaccgtataa gaagatggac caatcatgtt attgtaaaaa
    79801 agttcggcaa ttggtgaacg ccaatcataa acaagatttt catctgcatc atctgtgaaa
    79861 tttgctgtac cgatataaaa ggattctttt ggctcattat ccagaaagtc tacaacgact
    79921 tttccaaagt agggactttt caataaacgt tcgatctttg ctagctgatt ttctgcgttt
    79981 ttctgtttga tattcatctg atcgatttct ctatttttca tttcaatcat cgaaaaagta
    80041 tccaggttat ctaagtaact gtcaaaattc aatcgaagct cgccagccat ttctttcaac
    80101 gactggtttc cttctatttt tgccgtatcc atcgattccc gcagttcgtt ttttttatca
    80161 attagctttt gatacacgtt cttcagatat gcttcttcta actgtttttg tgtgttgacc
    80221 atacacattc tcctcttttt ttaaagtggt ggaaccgatt agtttacctc ttttaagagt
    80281 cgaatgcaag ctttttaacg agtatcattt gttaggatat atcagttctt caggtcggac
    80341 agtttgatat aaagaaaatg caccatcaag tttttttacg tggtagccag actgcttcaa
    80401 gattctttcc gctagatagc tacgtaatcc gctatgacag ctgacgatat attcttgttg
    80461 gctgtctaat tcatttaatc ggtcccgaag ttcattcaaa gggatatgtt tggcttcctt
    80521 gaatttgccc ttttccaatt cttctgctgt tcgcacatct aataaaattt ttccttttgc
    80581 tagttctgtc ggcaattcat gccattgtac ggtttcacta agcccttctg caatgttcat
    80641 tgctgcatat cctagcatgt ttactggatc tttggctgag ccaaatggcg gtgcatacgt
    80701 caattctaat tccggtaaat cgaagatcgt caaatgccct ttgattgctg ttgctaaaat
    80761 atcgatccgt ttatccacac cttttgcccc aacaccttgt gcaccataaa tctcgccagt
    80821 ctctggatgg aagatcaact tcagtaaaat atctgttgct tctggataat agcttgcatg
    80881 atcttttcct gatacatgaa tgacttcaaa agacagttcg ttttctttgg ctgttctttc
    80941 acttaaaccg gtggatgcgg cagtcattcc aaacgttcgt ataattgctg ttccaatacc
    81001 gccttgattt cttcgagaaa ttccggaaat cgtgtccgca acttgtctac cttgacgatt
    81061 ggctggtgaa gcaagagaaa taagtgcatc ttttccagtg atttgctgtt ttacaacgat
    81121 agcatccccc accgcataaa tatcaggaat attcgtttga taacgttcgt ctactaggat
    81181 acccccgcgt atgcctaatt cgattccagc ggtcactgct agctggttat caggacgaac
    81241 accaatggat aaaataacta aatcagcagg gatggtacgc ccatcatcca gtcgcaatcg
    81301 tttcccgtgg tcttcaaatc cagcgacagc atgagaagta atcacttcta ctccttgatg
    81361 agacaattct ttttctataa aagctgccat ttcttcatcc agtgttggaa gaatatgtgg
    81421 tgcttgttcg atcaccatga cttctaaacc tcttcttttt aggttttctg ccatttccaa
    81481 tccgatgaat cctgcaccaa cgatcacggc acgctttggc tgtttttcta atgcatgcat
    81541 cactttatcg atatctggta catttctaat agaaaaaaca ttgtcggctt ctgttatccc
    81601 tggaatcgat ggaacaactg gttttgcacc aggagataaa accaatgcat cgtactgttc
    81661 aatgtgtttt tgaccctcat gtacgacttc taccgttttg ttttcgggaa agatggctgt
    81721 cacttcatga tttggacgca catctaaaca aaaacgagcg gataatgatt ctggcgtttg
    81781 gacaagaaga ttttcccgtt cactgatttc tcccgaaaga taataaggca agccgcaatt
    81841 tgcaaaagaa acatacggtc ctttttcaaa aacaacgatt tctgcatctt ccattaatcg
    81901 gcgaagccgt gtagcagcag acattccacc tgctacacct ccgacaataa cgattttcat
    81961 cttatttccc tcctactgtt cgatcaaacc attgattcat gccaccacgt acattcacta
    82021 catcataacc agatttcttt aattctttag ctgcttgttt actcctcatc cctgattgac
    82081 aaatcacata gacggtcttt tctttgtctc catcatatct gtttatactt tgtaatggaa
    82141 catttttagc tccttttata tgcccgcccc ggtattctgc aggtgttcta acatcaagta
    82201 acgctggatt ttccaaaagc ttttccttta attctgggac tgttatcgat tttattgatt
    82261 gaaatagcaa attcattttc tgcacctcat ttacattttt ataaatacct catggggtat
    82321 attaaacagg agctttatta atatgtcaac cagaatgact cttcattttt ttcactactt
    82381 tttatatttg cataaaatac cgataagggg tattatctaa ttataaaaat agaggagaat
    82441 gtgttatgga gattgataat aaaaatatcc tgaaccgttt gaaacgtaca gagggacaaa
    82501 ttcgtggtat ccaaaaaatg atcgaagacg aaaaagaatg tatggatgtc atcactcaat
    82561 taagcgctgt tcgttctagt attgatcgcg taatgggcat gatcgttgca gataatctga
    82621 aaaattgctt tgagaaacct gatagtaatc cagaagaaca agcggctaaa ctagaacaag
    82681 cgatcaagat gattattaaa aagtaaagga atgatttcaa tgaaagtcgt tttccatatt
    82741 gatgaattag aaaaatggtc agaaacaggg aaaaacgtaa aaaacctgat aaaagcttct
    82801 ccagaaacga cgattgtcgt atctgtaaat ggcatcgcga ttaccggtta tctagactct
    82861 gccaatgcag aattcttaga tttgcaagga gtcgtttttc acgcttgcgc caatgctatg
    82921 cgtgctaatc atatttcaga aagttcttta cctgaacaag taatcgttgt accagcaggt
    82981 gtccttgatt tagttgaact gcaatcccaa ggttatgctt atatcaagcc ttagtcagta
    83041 gaggttgcga cagaagtgat cagcttcaag aaatttcggc ttatttttcg aagaagctac
    83101 ttctggaaaa ccgtgaatca aaggttgtga aaacgctgtc ctgcatcaag taataagaac
    83161 agccaaacaa aaatagcttc tcatattttt cgtttggttg ggcttattac cgcagatgca
    83221 agcttttgaa caccgtttaa ggaagaggtt gtgaaatcag cggtttgacc taagtattag
    83281 agaaaaaaac gggaaaatag ctcctcatat tttgccgttt tttgagctaa taccgaaggt
    83341 cagctgattg aacaccgttt accaagaggt tgtgagccta ccatttaact ccgaatcaca
    83401 agaaataatt tcaaaaacaa cttctcctgt ttttggtttg gttggcttat tatcgagaag
    83461 tagctactgg gagtctattt atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt
    83521 attttctttg aaacatcttt cggtactcga cagcatacaa gacaaaagat tgtaaaagac
    83581 cgtaagtgat gacggcaaaa atgactctca cccacagcgt tggtaattct gggaaaagca
    83641 gcaaaaggat catgccaaca tagatgacag cagtcgctac tttgccatac cacttcgctc
    83701 cacttaattt gatattttta gatagcccga ttaatccagc tacaaacata aagccttctt
    83761 ttatcaagaa tagaccaaac aacagccaaa gccacggtcg ataccatgcc atagacaaaa
    83821 tcagaaaaag ttgtgtcaat ttatctgcaa acggatcaag caccttccct aagtctgtta
    83881 cctgattata tctccgagca atgaccccgt ctagataatc agacaaacct gagacaacaa
    83941 tgaccgctcc tgctatataa aatgactgtc tcagcacaaa ataaagataa atcggcagca
    84001 aaaaaatacg gaatatcgat aaaagattcg gaatcgtttg tatttcttta cgccagtctg
    84061 tgcttaattt cattttatcg ccctacctcc tcagcactct tttcttttta ttttagctta
    84121 cacggggaat aaaaccaatg atttcactgt ttttattttt ctttacagcc actgatatta
    84181 tgagctaatt tgagataatc acttgctgtt ttcatcgttt actcttcttt cttcctgtcg
    84241 tttgcgcatt tcttttacta gtactttatg agcttttttt gatcctcgct ctaggtcacg
    84301 ctgccgttta cgtgttttat aatcgataaa cagactgtct aaatcgtaac ctgtcgggta
    84361 cagatcttct gctgaacgca ccagacgaac acgctgacga ggcacgtgga ttattttttt
    84421 gtctaaatat acatcaatcg tatcttcacc tatatcttca taaactaacg cttctttttg
    84481 tggtattcat tgacaaatat ccgatcacct tttgcaaaga gctggcgttc ttttttattc
    84541 tccttcctat ttgtaactgc tgtcttagga agaaattctt ttttctgtgt ccgataggct
    84601 ttttccgcca agtaacgctc tgcttgatgg atcaatttca tcgacatatc cattttatga
    84661 gcaatccaca aagcttggct ttctcctgtt tctccaaccc gcaactgata tttaggacga
    84721 agtgcttccc gatcgaaagc catagcagcc ggcacaaaat cttcatgatc tccggcgaat
    84781 ttcttgattt ctccataatg ggtcgtggca atgatcaatg ccccttgctc atacatagat
    84841 tccatgattg cgatcgctag tgcagcccct tcatttggct ctgttccact gccaatctca
    84901 tctaaaagta ccaacgtatt cctttttatc tttttcaaaa tagcagcgat gttttgcata
    84961 tgaccagaaa atgtactcaa cgcattttct aaattttggt ggtcaccgac atcgacgaaa
    85021 atctcatcaa agacagcaag ctctgtccct tcttttgccg gaacttgcat accgaacatc
    85081 gccatcaaag tcaataaacc gactgtcttc aacacgactg ttttgcctcc ggcatttgct
    85141 ccggtgataa tcaagcctcg atagtcttta ccgatttcaa catccaatgg taccgcatgc
    85201 tcaggtaaaa atggatgtct gccttgcttg ataatgatat gttccgattt attgattcta
    85261 ggtgtgcttc cattgatttc tcggctaaat tttgcccgag caaagatgat atctaacacc
    85321 gtgatcgttt cgatcaataa atcgatcact tcttcgtttt ctgcgatagc accagtcaat
    85381 gccgccagga cttgatattc ttcactgatt tcttcagctt ttaacaactg atagtgttca
    85441 ttcaatttag aaatcacagt tggttcaata aaaaccgtcg ttcttttatt tgattcatcg
    85501 ataatcgttc catctatttt atttttatag ctggctttga taggaatcgt atagtattcg
    85561 cctttttgaa caatcattgc ttcttggatc atttctttgt ttttcggatg acgcaagaat
    85621 ttcagtaatt ttgactggat ttctttttcg attagttgta attgtttgcg tactttacgc
    85681 aaattacgtg acgcgtcgtc actgacttta ttatttttta ttttctggtc aatcaactct
    85741 tctacattta tcaaatcagg aagatgtttg ctataagcaa acaacaaggg tgtctggtat
    85801 tgattcttat caaaaaactt tgtgatcatc cgactgcttc taagaaaatc cgcatattcg
    85861 atcaaatcgg ttggctgcag aatcaaccct tttttgactt gttctgtcag tgcatcgatc
    85921 ctcggcaacc ccataaatgg aacatgctga ctgctttcta agatcaagcg tgcttctctt
    85981 gtttcttctt gacgatccaa gacagtttgt aggtttgtcg agaccgtcat atcgctgatt
    86041 cgttttttgc tatagtttcc aattgctctt gcttggattt cctttttgat ccgatcaaat
    86101 tgtaattgac taatcatttc gttgtccata ttctctcctt ttcttgaaga gatacctgtc
    86161 ccaaaataaa taagggccta gagataaata aaaggataga acacagctta aaaaagtcat
    86221 gtcctatcct tattagaata caaagatatt gttttcctga caggtaccgt ttcataagca
    86281 caccaaataa actttgttta ttggctattc gacttactga ttagatacca gctacactca
    86341 caaaacatac cccattcttc taggtcttat ttattttcca cttaactata gcaagacaca
    86401 ttttggttgt caaactttag tatgttttat agatttacac cattaatgaa tagacaccta
    86461 ccgcgataat tgctccgatc attggacctg cgattggtac ccatgaatat gcccaatctg
    86521 aagttccttt attagctatt ggcaataccg catgtgctaa tcgaggacca aagtcccgag
    86581 cagggttgat tgcatatcct gttggccctc ctaatgaaag accgatggct aagatcaatc
    86641 cacctacagc gaatacgttc gttccaggtg caaattcgtt ttgtccaaaa gctagcaacc
    86701 caagaactaa tacaaatgta ccaattaatt ctgtgatcac atttgctgga taattccgta
    86761 tagcaggacc tgttgcaaac gttcctaaaa tcgcgctttc atctttcgtt gcattccaat
    86821 gtggcaaata agaaagccaa acaagaatgg cccctgcaaa agctcctaaa acttgcgcaa
    86881 taatgaaagg aacgacaaga ttccaactaa ttgcccctgt catcgccata gctagagaga
    86941 ctgcaggatt caaatgagct gggcccatga atccagaaat atagacagcc attgttacag
    87001 cgagtcccca accaaatgcg atgacaatcc agccagaagc ctgtgcttta cttttgttta
    87061 aattcacagc cgcacacaca ccatctccaa gcaaaactag gatcagtgtc ccaataaact
    87121 cccccatgat ttgagtcata tcactgttca tttatttctc cccctttagt tcccgcagat
    87181 ctgattctgc aatcaatgct tctaaacgtt tagtctgttg cgccttctcc tcttctgtcc
    87241 attcaaaata tccagcaatt gcatcgatca caggttgctt gatctcatca agctgatctc
    87301 gttcaaacaa cagatgattt gtacgacgaa tgagataatc gcctggggcc aaaaccattt
    87361 cttcttctaa accataacgt aaccgagctg actcagcgag actcaaacca ggataaggtg
    87421 ccatttcttt tgctaattca aagatacgtc gggcattagt cccgtaaaaa tcagcgatat
    87481 aagtagcatc ttcttcctct aaaccggctg ctactccttc ttttgctaat tctgtcactg
    87541 tttcttctaa tttagttgga tcgaagtttc cacctgaaat ctgatatttt ttagagtcga
    87601 tttctttcgt ctctattccg tattcttctt ttaataattg gcgtatcaat cgcaaagctc
    87661 cttctgccat cttacggtaa tcggtgattt tcccaccaga taaagtcagc aaaccatctg
    87721 gttctctttc taaagaacta cctctggaaa tcgtagaagg tgctttttca tcacggctgt
    87781 tttccaaatg gttcaacacg tcttctactt cagcacgaga aactttgttt tccttatatt
    87841 cacttactgt atcgacaact ttattgaagc tcttgtctga aatcgatcca ttatctccac
    87901 cattgtaatc agaaccagaa ttaccaatca acagtggacg aagtcctgcc cagcttgctt
    87961 cgatatcatt caatgtgata tttgcttctg gatagcgatg attgatcaca tccaatagat
    88021 aatctacatc ttcttgtgtg actttcggat ccgtaaaatc tccttggtaa tccgtatctg
    88081 tcgtaccaaa gtaagtcttg ttttctcttg gaatagcaaa aaccatccgc ccatcttgct
    88141 ttcctgtatc gaagtatgtg ggttgcggta caggcagttt tttcgcatct acaactaaat
    88201 ggatcccttt agttggacgc attttaggag agactggacg cgtaaaattc aagtttcgta
    88261 ctttatctac ccaaggacca ctcgtattga tcactaattt tgctttgatt tcgatcactt
    88321 catctgtcag caggtcacga gctttgacgc caacgatttg atcgccatca tataaaaagc
    88381 caaccgcttt cattttactt actagataag ccccatcttc ggcagccttt ttgatattat
    88441 caatcactaa acgggcatca ttgttgcgga aatctagata gacacccgcg ccttttagcc
    88501 cttctttttt caaaaacggt tctctttcca atacttcttc tggtgtcagc gtataattct
    88561 catatttggt tcctgtcaca tttgccagtt tgtcataaag gtccattgct actttgaccg
    88621 agaacatatt gaaggttgtt gctccttcgc cctcatagat tggcaaaagc attggatctg
    88681 gttttgggat gtgtggggca atactttgta cgactgcacg ttcaccaact gtgtcagcta
    88741 ctacttccac atcaaatgtc ttcagataac gaataccgcc atgaacaagt ttggtcgagc
    88801 gagaggaagt cccttctgca aaatcctgca tttcgatcaa tcctgttttg attcctgatg
    88861 ctgctgactg gactgccaca ccggcaccag tgattccgcc accgatgatc aacagatcca
    88921 attcttcttg ctgcattttt tgaatgctat cttgtcttgt cttgtttgaa aacatatgga
    88981 tttcctcctt attcaaatgc ttgggtagct tttactgctt ttttccaacc gttgtaaagt
    89041 ttttctcggc gctcttcctc catttgaact tcaaatagct tgccagcttc ataaaattcg
    89101 cggatctcgt ctgtatcttt ccaataaccg acagctaatc ctgctaaaaa tgctgcccct
    89161 agcgccgttg tttccaagtt ttctgctcgt tggatcggta cattcaagat atctgtttgg
    89221 aattgcatca gatattcatt gtttgctgca ccgccatcta ctttcaatac aggaatcttg
    89281 attccagtgt catcttgcat cgtatcgatg atatctctca cttgataagc aatcgactgt
    89341 aaagtagctt taatgatatc ttcttttgtc gtaccacgtg tcaagccaaa catagcccct
    89401 cgtgctgcct gatcccaata aggagcacct agcccaacga atgcaggaac aacataaacc
    89461 tcatcttcgc ttgtagagcg tttcgctgcg tcttctgaat cgctagcttt ttgcaacatt
    89521 tgaagtccat ctctcagcca ttgaatcgaa gaaccagcca caaaaatact tccttctaaa
    89581 gcatagtaaa cctttccatt tatgccgtaa ccaattgtcg tcaataaatt atttttggat
    89641 aattgcggtt tttcaccggt attcatgaca ataaatgaac ctgtaccata agtattcttg
    89701 accatgcctg gttcgaatgc catttgtccg aataatgcgg cttgctggtc tcctgccatc
    89761 cctgaaattg gtgcttcgct tccataaaaa tgataacctt gcgtataacc ataaacttct
    89821 gagttagaag tgacttttgg taacatcact cgaggaatat ttagtaaatc taaaatctcc
    89881 tgatcccaat ctagctgatg gatattgaac aacatcgtcc gactggcatt cgagtaatca
    89941 gtaacatgtg cttgtccatc ggtcagtttc catactaacc aagaatcgat cgttccaaac
    90001 atcaattcgc ctttttcagc tcgttcttgc gcgccttcta catgatcaag aatccagcgt
    90061 atcttcgttg ctgagaaata ggcatcgatc actaaccctg ttttttcgtg gaaaaattct
    90121 tgatagcctt cattttttaa gttatctgca attccagctg actgtctgga ttgccaaaca
    90181 actgcatgat agattggacg tcctgtatct ttttcccata ttactgttgt ttcacgctgg
    90241 ttcgttatgc caatcccagc aatctgattt ggtttgatcc ctgattcgat gaacgctccg
    90301 gcaataactg actgtacaga attccagatt tcatttgcat catgttccac ccaactttct
    90361 tgcgggaaat attgcgtgaa ctctttttga gaactgccga tgtgtcttcc tttcttgtca
    90421 aagataattg ctcgtgagct agttgtacct tggtcaatcg ccatgatata cgttgattct
    90481 gtcattgtga ttccctccat tatatgcaaa cgctttcttt aactactctt atcatactac
    90541 tgttttaaaa aaagaaactg tcaaaatttt attctttttt tctttttttg acagtttttt
    90601 attatgaagc tattttttaa tcaattcttt tcttttttca gcgtctctat tcttcttttc
    90661 aatcgaataa tgtcgtaagg tgacgcaaat tctgacagat aatattcaca ctgttttttg
    90721 tttctctggc aaaatgtgat caccaaatca tattctttac cttcttcata acttccaatc
    90781 tcaataccgc cgattcccct cagttctctg atcagatatt gttgaaaagc aatacgataa
    90841 atcggcagac tttctaaatc gattccaatc actactggtt ttgcaatata aaaatcgatc
    90901 atcaaaagaa cattggcata ctggatcatc aggtaactaa gctcactatc ttcagctctt
    90961 ttcctcggca attgctgcac cgctgtttcc tgtaatttcg ccagtaactg tgttaaggaa
    91021 tgcccgagca ttttctgctg ctgttccaaa agccgatcac gctcatagac ttcgatcttt
    91081 ccaacaaaga aaaaccattc attgttgatc tgggaaattt ggtagcctac tgccttttct
    91141 tctttgattt tcaaacgacg aggacgataa tgccataaca tcgtttcccg aatgtaagta
    91201 tctaaaacag cggttggtaa ctttttgctc cgtgtcaaat catactgata aaatatttct
    91261 tcgtctacta tcgaaaaaga aacaaaaaag ctgtaaaaca tcattgtttc gtatacattc
    91321 agttctgctg ctgttcgact aagatgcatc acgatgattg gatcgattgc ttgataaagc
    91381 cggtccgact gatagagtaa tttcttttct ttcaaagttg cgtacgtact tttttcattc
    91441 aatagtcttt ttttcgtaat cccgagccag caagcaatct gtgcttctgc tgatgccgta
    91501 aacgttgttt ctaatacacg gtccaatcct agaatgaacg gtcttttttc tggtgtattt
    91561 tgcaaaaaaa gcggtcgctg atcttccggg atatattgga atagttcata ataaaaatat
    91621 cgtatctgta gctcttctcc gtataattga ccgtttttga tttgtaatcc aaattcctct
    91681 aagagctggt tcaattcttt gatctttcga aaaacagaag actcactgat attaaacgct
    91741 gtcgctaatc gaacgatgga tacttgctga tgttctaata aatagacgag catcttaaac
    91801 ttcaagcttt cctgcaataa aaacaataga attttttcca aacttacatc ttcgtccaac
    91861 acaagcagcg cctgatactc atttcgctgt acttctactt ttttttccat cattcggcca
    91921 atcaattcaa tatcttccaa atattgatca aacgaggctt ttgacagttg gacatgctct
    91981 ctcaaaccat tgatagaggc tgttccccct tgcagaacaa ggtttcgcaa aatcaggatc
    92041 tgtaactgct cttttctttc taataatgct tcaatcttca taggcagtat tcgccacctt
    92101 tgaccacacc atatctggca cacggtattc tggctggaaa aatagctcat gttcttctaa
    92161 ctgttcaaac gtccatcctt cttctactgc aattgtcagt tggtcaagaa ggtggaacat
    92221 ctcttttttg ctttggagct gtgctccaag aatctttttt tcagaatcaa atagaaattt
    92281 gagtttaact ttgctttgat ttattgctgt aagcttgtag tctacgtatc ctgtttcaat
    92341 tttttgggga taaaaaatgc tctcttcttc tgtcaaacct acgcttccta agaaatagcc
    92401 aaaccactga ttgccgactg gacgataggt tccctgatcc tttgtctgtg agccactaat
    92461 tgtcttgctg acatttcgtg ctgtacggac agcgttattt actagagaaa catacatttt
    92521 ttcatttgtt ggacgaaaag ttacttggat cgcatcgcca atcgcataaa cgtctttttg
    92581 agacgtttgc aagtagtcat ctacccagat cgttccatca tcattcaatg ttaattgttc
    92641 ttcccacatc tgattgtcgg gacgagaatg attagccagc aaaacaagat catagttctc
    92701 ctttcttttt tccgtctcta caatagcggt ttcattttcg gtcaaagaca caacttgttc
    92761 ttctaaaaaa agtgaaagcc cttgattttt catttccttt actaacggtt ctgtcatttc
    92821 aggatctaga taacgaaaaa gaattgactt tcggctttcg taaagatgga tttgtttttt
    92881 tcttgatgcc aacccttcag ctatttcgat tcctgcttgt cctgcaccaa taattgcaat
    92941 tttctttgca ttcatgatct tttgctgaag agccgttacg ttttgtatct ttttaactgg
    93001 gtgaataagc gttgattctg cagaaatatt tcgaaacttt tgatttgaac cagtcgctag
    93061 aatcaaacga tcaaaatcca atttttttcc tcctgctaaa acgacttttt tttctttcaa
    93121 tcctattact gtcttttctg tgtaaacatg gattcgatag cggtcagtca gttcttcttt
    93181 cgtgatccaa gaaagttgtt tttcactggt aacctttcct tttaatatca aattgaaccc
    93241 gctgggaatc catcctaact tcgattgctt ttcaatcaaa tatatttcac ttgttggcat
    93301 caattggcga agataaatag ccgcatgaat ccctgcaaac gacccgccga caattacaat
    93361 gttcatctgc attcctcttt cttataaggt gttctactgc taacttctca atcagaaaac
    93421 acagtattca taactcgctt tcaaccttat gatacatagc gatgcagcag aatcaaccct
    93481 ctccatcata ccgtgttcta ggtctgcttc tcaataatgt actagtggac ctaattgaag
    93541 agtatcattt ctgaagagat gtttcaaaaa atgtactctt ataaaagaaa aaaactcgcc
    93601 ttttacgtac ctctgtccaa ataggaaaca gaaaagaacg aaaagacgag ttttagcgtg
    93661 tgtaatgtaa tagttagaat agacacatca tttattttta attctttaat gcttcaacaa
    93721 ctgcttcgcc taattgatga gcagattgag gattttgccc tgtaagaagc cgcccatcaa
    93781 tttgaaccac ttctgaaaaa gctgctccag actgatagtt tgctcctttt tctttcaaag
    93841 catcttctgc taagaatggt acttcattcg ttgtaccatt tgcttcctct tcttcattac
    93901 tgaacccagt cactgtacgt ccagctaaaa tagattttcc agactcatct gtaactggca
    93961 acaatcctac aacaccgtga caaacagctg agaccagccc ttcatttcga tagatttgtg
    94021 aagcgacttt cccaagatct attgcttctg gaaaatccca cattgttccg tggccaccag
    94081 cataataaac cgctgcataa tcatttgcat ccacatcatc aggacgtaag gtatttccca
    94141 aggcccgatt tctgaaatct tcatcttcat aataggccca atcgatttca tccatattca
    94201 aaacactttg cggttctaaa ggtacatatc cgcctttagg actgacaaaa tctgcatcga
    94261 tcccattttt tactaataca tcataaaaat gagtcagttc acttaaccac aaacctgtag
    94321 gacgctcgat cttttcgtac ttttctacat ttgttaatac gatcaatact ttcattttcc
    94381 catcctcttt ctataaaaat tgcgttattg ataacgtgct ttcaagaaat tatataatgg
    94441 gcttggcaaa taaagctctt tatatgcttc ttcaaaaggt ttcgttacaa attcatcctc
    94501 acggttttcc aataatttct ctgcccagtc aggatcgacg atcatttgct gtccaattgc
    94561 tgcaagttct gcatatttca atacctgctc tacatcttcg cgattgcgga tcccaccgac
    94621 accaactaaa gggatctttc cttgaatcgt gttatggacc acttcaagaa tcgatttatc
    94681 gttgtattta tcagaacgag ctattcgatc atacatattt aatgaaacat gcagataatc
    94741 tagttttgac tcacgtaatc gttcaagaag ccagatcgtg tcttcaaaac ggatacctgg
    94801 tgtttcatat tcttctggtg agaaacgata ccctacgatg aatggttttt tagcatattg
    94861 ttcaaccgct gagaaaaccg ttgttaataa ttcttcaata aaacgataac gattttccaa
    94921 gctaccgccc cattgatcct ctcgtctatt cgaatgcgga gagaaaaatt gttggatcaa
    94981 atacgtattt gctccatgca actcgattcc atcaaatcct gattcaatcg ctctacgtgt
    95041 ggctttgcca aatgcttgga ttgtttcaac gatttcttct tcactcattg ctcgtggtgt
    95101 ttccgcatca ggacgttttg ctgcaactgc acttgcagat acaggctgtt ctccagataa
    95161 agttgctcga cttgtcattc gtccaccgtg gaaaatctga ataatcgctt ttgcgccttc
    95221 tccttggatt gctttcgcta gctcacttaa tcgaggaatc atttcatcat gagcgatgct
    95281 gagttcacct ggccagcctt ttccgatatc ttggacattt gcagcaccag taataacagc
    95341 accaagacct ttagaacgtt gtttataata agtgatctca tcttcggtga tcactccatt
    95401 gaaaaagctg agttgtgtcg tcataggcga cagcatcaat cggttcttca attgaactcc
    95461 tgaaggtaaa gtaattgatt gatcaaattg tgacattgtc attcctccca taattcgctc
    95521 tgattaacct atcgagaatc atctatcatt aaaccactcg attagttgta catgcaacta
    95581 atttgcttaa aaaattttat tttttttgta acttatctgt tgcatcattc attgtctggg
    95641 ctaattcttt acttgcttca tttcccaaaa ctgctttgta tgactgatga agttcttccc
    95701 agctaccatc gacttcctta gcaaattggc gtccttgttc tgtaagataa actaatgaca
    95761 agcgaccttc gctaactgtt ttgacgtatc ctttatattc taatttttca acaaaacgag
    95821 caatcgttga tggggcggaa tagagcattt gtgcaatatc tttttgtggt attccatctt
    95881 tttcttttaa tatcaataaa ataaaaccat aagttggtgc cagacctgtc cttctaaacg
    95941 cttcatcagc taatttattc aacatgcgat ctagcttttt cacgctaaag aaaagacatt
    96001 catctaacgc tttttcaatt ggcttttcca tttcatcccc tccattggcg tacatatcat
    96061 agtatgattt ttattttttg tcaaaaaaca cggaataatg tccgcctcct ctgtctagat
    96121 ttgaaaaaaa gaagtagcca tcttcaaaga atttcggctt gttttcgaca aaactccttc
    96181 tggaacaccg tgaatcagag gttgtgaaaa agtcgtttag ttttgagtat gaagaaaaat
    96241 tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga aaaactggct
    96301 tttgaacacc gtttatatca gaggttgtga aaaagccgtc ctgcatcaag taataagaac
    96361 agccaaacaa aaatagttcc tcatattttg tcgttttttg agctaatgcc gatggtcagc
    96421 tgattgtaca ccgtttattg agaggtcgtg agcctgccgt tgaactccga gtcacaagga
    96481 gcaatttcaa aaaacagctt ctcatgtttt atgactatgg tgacttgtga tcgaggagtt
    96541 ggcaggagaa tactgtttat tcgtaaagaa ggggctgtga caagactttt gtcacagtcc
    96601 cttatttcct tttattatag gatttcgcca tttgattgga tgacttgtct gtaccaatca
    96661 aatgattttt tctttaaccg tttctttgtg ccattccctt cgtcatcttg atcaacatag
    96721 atatatccat aacgttttga catttgtgaa gtagaagcgc tgacgatatc aatgcagccc
    96781 cacgacgtat aaccaaataa atctactcca tctttgatag cttccttcat ctgctcaata
    96841 tgctgcttca aatagtcaat gcggtaatcg tcttgaacgg ttccatcttc ttctactttg
    96901 tctatagctc cgagtccgtt ttcagctatg aacagtggtt tttgatacct atcatataat
    96961 tgattcaaag caattctcaa acccttagga tcaatctgcc agccccattc acttgatgga
    97021 agatacggat ttttaattcc tccagttatt aagtttcctt ggaccccttc ccaattttct
    97081 ggatgagcac tgactgcact agacatgtag tagctaaaag aaacaaaatc aacagggtag
    97141 gctgctaaga tttcttcatc acctacttct atttttaagt tgacaccttt ttcttcaaac
    97201 attcgttgcg tatacgctgg ataatatcct cttgcttgaa catcactaaa gaacaaagaa
    97261 gttctttgct gctcaattgt tttttgaata tcatccggat cacaagaata cggaacaaga
    97321 agctgatagc tgatcataca accgacttgt gaacctttga taatttcatg acaagctttt
    97381 gtagctaaag cacttgctat aaactgatgg tgtgcacctt ggtatttcgc ttgttccaaa
    97441 tggggatggt tttcttcaat tactcctacg ctaacaaaag ggtgatgttt tacacagttg
    97501 atctcattga aagtcatcca atattttact ttatttttat agcgtttgaa tactgtttca
    97561 gcgaaacgaa cgtaaaactg aataagcttt cgattaggcc atccaccata ttctgtaacc
    97621 aaatataacg gcatttcata atgggaaaga gaaacgactg gctcaatccc atattttaga
    97681 cattcgtcaa acacttgatc ataaaaaaat aatcccgctt cattgggtgt ttcttcatca
    97741 ccatgaggaa aaattcttgt ccatgcgata gacatccgat agcatttaaa acccatttca
    97801 gcaaataaac ggatgtcttc tttaaagtga tgatagaaat catttccgtg tcttttagga
    97861 taataatact tgctgttggg atcctgcgcc gcttttactt gctgatgcgt catcatatgc
    97921 cattgatctg tccatttttc tttaggtaaa tcgctgtcga atgtatacac atcagacaca
    97981 gataatcctt tttcatctac atcccatgca ccttcacatt gattggccgc tgtcgctccg
    98041 ccccataaaa atccttctgg aaattttttc atatcgatcc ccctaaactt gctagttttc
    98101 ttgtttgatt atttttctta tgactagtaa tgatattttt gaatctacta ctatttgtaa
    98161 caataaccat tgtggtcgga tcatatccct ctttgattaa ttcttgcaaa tctacttcca
    98221 tcaataaatc gcctttacgt actttttttc catcagatac tgcactttga aagtatttgc
    98281 catttaaatt cactgtatca ataccgatat ggatcagtat ctctgctcct tcttcactga
    98341 ctaccccaat tgcatgtttc gttggaaaaa ctgttgaaac ttccccgttt acagaactgt
    98401 agatttttcc gtccgttgga atgatcgcaa acccttctcc taaagatcca ctagcaaatg
    98461 ttggatcgtt tacttcagat aaatcaatta tttcacctgc tgcaggagta cttaacacta
    98521 tatcctttga ggattcttct tcgatttcat ccttgtagcc gaaaagaaaa gttagcatac
    98581 aggaactgat catcgctaag aaataactag caactagcca aacaaatcct tcaccaatat
    98641 aaatcgggaa agacaaaata cttcttgtag caactgccaa agcaccggct cctgcggcac
    98701 cagctactcc accaaatata gctccacata tacaagcaat gtaaaatggt cgtttatatt
    98761 taagagtcac accataaaca gcaggttcag tgattccaaa taatgctgtg ataaatgcag
    98821 ataaagataa actttttact ttcttatttt tcgttttcaa aaatacccca agtactgctc
    98881 ctgcttgcgc agctacggca gtcatacatg ctggacctag cgtatcacga ccgtagaaag
    98941 aaagattgtt ggtcattact ggtacaagtc cccaatgtaa cccaaaaatc actaacactt
    99001 gccaaaatcc gccgataaca gcaccagata aaacagggct aaagttataa acagctaaat
    99061 atccagctgc aatcagctga cttgcataat ctgcaattgg acctacaact aataaaataa
    99121 ttgggaccat cacgatgata caaatgaatg gtgtcaataa atttcgaata ctttcgtgca
    99181 agcttttgcg aatcttaggc tcaatataag aaagaaccca tacagctaaa attgctggaa
    99241 taaccgtact tgtatacctt gctagaatca cgggcatccc tagaaatcgc aaggttatgc
    99301 tatcactata tgcactgaca atcgtaggat aaacgagagc tgctgcaata accatagcga
    99361 tgaaacgatc tgtattgaac ttttttgcag cagtatatgc caaaaatata ggcaagaaat
    99421 agaaaaatgc atcagctgct gcattcaaaa tgatataagt tcctgattca gtagttaata
    99481 aattcaaact cgtacataag gcgagtactc ctttgatcaa accacttcct gccaagacgc
    99541 ctaaagtcgg ggcaaaaaca gcggcgatca tgtctataaa agagtttaaa atgccttttt
    99601 tctcagttcc tctctttcca ctagatgcat catcttctag tttcatttgt ttggataata
    99661 cgtcatacac ttcatttact gcatttccta ctacgacttg aaacattcct ccagcttcga
    99721 ctacagtgat cacaccttct agttgctcta ccttctcctt atctgccttc ttattatctt
    99781 ttagggtaaa gcgtaacctt gttgcacaat ggtaaacatt gctgacattt tcttcgcctc
    99841 cgacaaatcg gacaatatct ttcgctaatt gtgaatagtc catttttact tcccccttgt
    99901 taattaatgc cgtagcaatc cttcttacaa gataagtata gttcttagaa aacggtttct
    99961 ccattcaatt agtacccctt ctaatagcga acgaaactgt acaatagtgt gtatggttta
   100021 cttagccgag caaatcactc actgcttgaa agaaaacaag tatatcgttt ctcttatagt
   100081 caaaggagac aaaaaaaaca cgcttgatct tcctttgctg atgtataagt ggtatctcaa
   100141 gctgatactc tgtgattttt gtttctactt ggcttttgat aagacagcac aacaaagtct
   100201 cggcatttaa cggaagcata actaggtcag aactaggaga gattagttgc tgaatggaat
   100261 ctatcttttt atggattttt atttgatttt cttctttgta gtaatgatca agcagttttg
   100321 ttttcttttt ttcttcaact atggtcagct tttcacgcaa aatctgataa atctcccaag
   100381 attcaaactc atctagaaca ctaggtagat aaatcaaatc atcccactca aaaataagct
   100441 cttgctcagt agtcaaaatt gcatcatatt ccgttatttt gtttgattga tttttgaaaa
   100501 gatacactgg ttctatccgg cattctttga tccacatgtt catactttgt tgaatctgtt
   100561 tttttagtgt attcaaaata ctaattggct ggttactaac aatcagtatc tccccttgtt
   100621 ttcgaaaagg attcaacgct tcggcaagat acaaaacaaa ataagcgctt tcagctaaat
   100681 tgggacgtaa ttgaaaatgt attgggaaga aacgccgaat taaataaatt tctaaaggat
   100741 aacgggagat gatcttttgt acaaaatgat tcatggtatt atgtccggca tccatcctcg
   100801 ttttcatttg tttgatatgg gttagcaaaa attctgtttc atggaacaat aacgaaacag
   100861 gaagatctaa aaaacgaata atcgtttgct caaatgctct tagattcttc tctatttctt
   100921 tgtttttttc atcattaatc atcaaataat ttaactctaa tagtcgattt tcaattgctt
   100981 gtttttcttg attggaaaaa gaatagggta gttctgatga taactttttt gtgagggtct
   101041 ctaccatcgg aataaataat gtatacctgt ttgttttttc taaaagttct tctcttcgaa
   101101 gaatggtcaa aacgagatac cgagaaaatc gtatgaagtc ctctcctgaa acaatataat
   101161 tatatgatat aagaacctct tttaatgtct tttggacagt cggcatgata ttggaaaaaa
   101221 ttgtactgat ttgttgagga aaccttgcgt gttggatcac cgtttctgtt ccaacaagag
   101281 ataagaaaat tctttttgaa ttttcttcag caatgatttt tagtcctttt gtcgacgaaa
   101341 cttccagttc taatttagga ttgcgctcga tccaacgcaa aatcgttttt atttgtaatg
   101401 atacggctgt ttttgaaaga aagaaaatgt ctgctatctt ttgcatactg acataccctt
   101461 tttgaaacaa taagtacgtc acaatagttg aagctcggaa atccatgctg gaatagattt
   101521 tatgtttcgt cacctcttgt atgatttgct gtttagtttc agattcaatt gattctaaaa
   101581 aataacctga acgtttattt gactggataa tgattttctt ctcgtagata gcaagcatat
   101641 ttttgatttc agtttggatc gttttcttgt tggtacctaa agcttcagct aattcttcgc
   101701 ttgttatcca atgattctta tttcttaata ctacaagaat ttggagttgt cgtttattta
   101761 agtccatttg cttacacctc ctctttgtta taaacataca ggaaaaacct ctcaaatgac
   101821 aatagtcact tgcagaggtt taaaaatcat ttgaagaaag atctatattc aaggttttta
   101881 ccattagact ggataatttg tttataatat tcaaaactat cttttttcaa tctcctaaag
   101941 cttccgctcc catcattttg tctatccaca taaataaagc catatcgctt cctcatttcc
   102001 cctgttcctg ccgatacgat atcgattggt ccccaccata agtaaccaat gatttggcaa
   102061 ccatctttta ttgcctcagc aatttcctga agatgtattt ttagatactc acaacgtttt
   102121 ggatcgtgga ttcgattcaa ttcatctggt gcttcatcta gtccaatgcc attttcaact
   102181 atgaataatg gtaattcata acggtctgtc aattcattca tgacataacg aaaccctaga
   102241 ggatcaattg gccaactcca aggttcggga gatttttcct tcaaaaaggg attatcgaaa
   102301 ccatttgctc cgccagtatc tacgcgcatt tctgcttctt ttttatagac agcgctacga
   102361 taataactaa atgcaatata atctgccgtg ttagcttgca agcactgttc gtctccatct
   102421 aacattactg gtgcacagtc ttcttccttc catgtcctgt aaatataagc tggataatgc
   102481 cctcggcaca tgacatcgga gaaaaaccat gaattgcgtt taaattgcag tgaaccaaat
   102541 acattctctg gatcacagtc atatgcataa gtcgctaatg cagaaaagct acacataata
   102601 cccatcttag catttggatc gattttcttt aataattgag tagcacgggc gttcgctaga
   102661 aacatatgat gacaggcttg ataaatcact ttttttgtta gtgtagcaat gggttcaagt
   102721 ttatctctag cagggaatgt aaccaatcca gcagctgcat aaggcatacg aaaagcatta
   102781 ttgatctcat taaaggtcat ccaatatttc actttccctt ggtagcgttc aaagacggtc
   102841 tgcgcataaa attcaaaaaa gtcgattaat tttcgatttt gccacccccc atactctcct
   102901 accaatgcta agggggtttc ataatgacta agagtaatta ctggctcgat atcatatcga
   102961 tttaatgtat tgattagtct gtcataaaat acgagaccag cctcatttgg cttctgttcg
   103021 tctcctcttg gaaatattcg tgcccatgag atgcttgttc gaaaagcttt taaacctaac
   103081 tccttcagta aacgaatatc ctcttcaaat cgatgataaa aatcaatcgc ttcatgagaa
   103141 agataatgga gtgattcgtc taaagcaatc tcccaaatct ttttttcttc attccactga
   103201 atactaggat gctggttcat gatcccgata agcgtatctg tcacattagg taatttgccg
   103261 tcttccaacc atgcaccttc acattgatta gcagctactg ctccgcccca taaaaaattt
   103321 tctggaaata tttcctgatc cataactatc tctcctcgct atcaacaatt tcttacttga
   103381 agtatagctc gattcatcaa aacaagatat tctaactatt tcggcagaag agaagaaaaa
   103441 aattgaatgc tactcttcat ttttaatatt tatatataac aaatacataa gacgatcccc
   103501 gaaagaaaac actcgttaac catctaagca aacgatttat tttacttcca acacaaatgc
   103561 ttcataaggt cgtaaatcaa ctttgccgtc tggtaactgt ttcagaggat aatttgacac
   103621 aataaccctt aaagcttcac gtggaatagt gaacgtctgc ttttcactgg acatattaca
   103681 aataacgagc cattgctggt cttcatatat gcgagaatag gcaaagacat gctcaggcgt
   103741 cttttccact aattcatagt ctccccagac gatgatgtca tgttttctac gcaaatcgat
   103801 caatttcttg tacatataaa aaacagaatc ttgatctgct aacgcttctt ctgcattgat
   103861 ttctttataa tttggattga ctcgaagcca tggaattcca gtagtaaacc cagcatgttc
   103921 acgggcgttc cattgcatag gcgtcctagc attgtctctt cctttcgcct tgattgattc
   103981 caagatttcc tcttttgtaa atccagaaga aatcctttcg ttatacatat tgattgtctc
   104041 aatatctttt gcttccgcga tatcatcgat tgggcaattc gtcatgccta actcttctcc
   104101 ttgatagata taaggggtgc ctttcatcat atggagcaag atagcaaaca tcttggcact
   104161 ttcttttcga tacacatcgt catttcccca acgagaaaca attcgcggaa gatcgtgatt
   104221 attccaaaat aaactattcc atccttcatc acctaaagat gtttgccatt tggataatac
   104281 ttttttcagt tcagagatac tcaagggttt caaatcccat ttgtctttgc cttcttgctg
   104341 atctaatcct atgtgttcga attggaagac catggataat tcatttcttt taggattgga
   104401 ataaagttta gcgatttcgg tagttgctcc ccatgtttcg ccaacagtca tcagattttt
   104461 gtcaccaaaa gttgcttgat tcatttcctg aagatactca tgcagtttag gtccattact
   104521 tgtgatttct ttatccggtt ctttgccaat catatcgatc acgtccatac gaaagccccc
   104581 aataccttta tcgatccaga aattcatcat ttgataaatc tcttggcgaa cctgtttatt
   104641 tttccaattc aaatcaggtt gacgcttgct atacaagtgc agaaaatatt gccccgtttt
   104701 ttcatccaac tgccaagcag aaccactgaa tattgagcgc aatctattag gctcttttcc
   104761 attgaccgga tcacgccaaa tataatagtc ccgataagga ttttctttat ttttacgggc
   104821 ctcaatgaac cacggatgtt catcagaagt atgattcacc accagatcca tgatgatacg
   104881 gatatttctt ttctttgctt cttcaatcag ttcttccatt tcttccatcg taccgaattc
   104941 tgccatgatt gcttgataat tactgatatc atacccattg tcatcattcg gagactcgta
   105001 tactggagaa agccaaattg catcaatacc taaatacgcc agataatcca acctggaaat
   105061 aatcccccgc aaatctccaa taccatcttg atttgaatct tgaaaacttc ttggatagat
   105121 ttgataaaca actgaatggt gccaccattt ttgttccatg attttcctcc tataaatgga
   105181 tgtttctatt ttttgcttaa tcatttattt tagtctctct cactaaaaat tgtaacgctt
   105241 tcaaatagct attgcacctt gttatcggta acagaataaa acaaaaaaac agctgaggaa
   105301 attccctcaa ctgttttttg aaaggattga tttatgcagg gaagaatgct gatttcacaa
   105361 cttctttata aacggtgttc actcagctgt cccttggtat tagctcaaaa aacggcaaaa
   105421 tataagaaac tattttttcg tttttttctc taatactcag gccagaacgc tgatttcaca
   105481 acctctttat ttcacattca atatccacat gaatcccaca aatacaaaga acagaccata
   105541 catgattgga gatacttctt ttcctctttt agctgctaac atggtaattg gatagaagat
   105601 aaaacctaat gcaatcccat ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa
   105661 gaatgaaggg atcgcaatct ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat
   105721 caatactcct accataatca atgctggtgc agttacttgt gaagtaacaa ctgatagtaa
   105781 tggagaaaag aacaagccta acaagaagaa aatccctgta gtaatagctg tgatacctga
   105841 acgtcctcca acggcgatac cagctgatga ttctacaaat gcaccaacag gagaagtacc
   105901 aaataaggaa cccgcaagca tagctgttga atcagaagct aaagctttcc ctacacgagg
   105961 catcttgttg tctttcatga aacctgcttg gtttgctaat ccaactaaag ttcctgctgt
   106021 atcaaagaaa gtcacaagca agaatgtcag cactacgacc cacatttgaa gagaattgat
   106081 gtcccctaca tgttttaatg caactaaaaa tgttggtttc aaacttggtg ctgcggcaac
   106141 aatttcagaa ggcattggaa tcaaacctgt gaccagacct aaaatcgttg tagctgccat
   106201 cccgatgaag atgccacctg gtacacgccg aacaagcaag atagcagtta cgactaatcc
   106261 aaaaatcgtc aaccaagtag aaccaacgtt gagtgatcct aatcctacta atgtagattc
   106321 attcgaaacg atgatcccac cttcgctcag accaaggaaa gcgataaata gaccgatacc
   106381 gccagaaatc gcatatttca aatctgctgg gatcgcatca atgatcattt cccgcagctt
   106441 gaagattgtg atcaaaataa aaatcaaaga agcaacgaat acgccggcta atgctgtttc
   106501 ccatggaatc cccattccta aacaaacaga ataagcaaaa aaagcattga tcccaagagc
   106561 aggtgccgtt ccaattggat aacgggctaa tacccccatt aaaacacagc cgatcgcact
   106621 ggctaatgca gtcgcagtaa acactgctcc ttcatccatt ccagaagcac ctaatacagt
   106681 tggatttaca aataagatat aagccattga gataaatgtt gtaaatcctg ccagtacctc
   106741 acgttttacc gtggtgttca atttttctat ttcgaagtag gaaagtattt tttccttcat
   106801 cttccatccc cctaaatccg aacattataa ataaagtccg ttaaaatttc gatttattaa
   106861 taaaacaacg tgttttatca tatactaaaa caaaacataa gtaaacaaaa aattcggatt
   106921 ttaagcaaaa tccgaacgtt ccatctgatt aaatcccttt atctcaaagt tcatgaacat
   106981 tcactttgga ttacaagaat ttctttattg tgttaataaa gtttcttcat tctagtctca
   107041 aaaatcaata gtgtgatttg attttaccat ttcgtttctg ttcttctttg atgaattctt
   107101 ttgtatcgac aggattcaga ttatttagtc cgccaaaacc tgtatcgacc atttttcccc
   107161 agccttttgt cttctttttt cctgatttga gattctttgc tcgaagcttc tcactatgtt
   107221 ttgctcgccg atacttcaaa taaccttcta atcttcccat tagatccact gtttttcctc
   107281 ctttttattc aatcagacga ctcagctttt cctataacaa aataattgcc ttcagggtca
   107341 gaaaaattga aagtcaagat gccattgtta tctgtgattt ctcctggttc ttccatgttc
   107401 tcgtagatag tttcaaattc atccgtaaaa aatactaaag aaggcggtgg tcctaatacc
   107461 tctggtgaaa acttctgcac aaatactttt gggaatacgc ctaattctaa ttcttttgag
   107521 atgctgagta caatactctt aaattcttct ggcaattcga ttgtttcaga aatttctgca
   107581 ccaatttttt ctacaaagaa tcggcaaatc atctctacgt cattgacata aagcatcaca
   107641 cgtgtttttt gtatcatcat ttgacccttc tttctatttg attttctttc ctgtctataa
   107701 actgattcca ttatatcacg aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa
   107761 taaagcaata gtttctagga ttgctttcct ccacaaactt tttttacaaa aaaaaaacgg
   107821 caatgaatgc cgctcttctt atttcgattc ttttataatt ctgcttctga ttcatctttg
   107881 gttagtgtgt gtctaacact agttacgcca aatgtttgat cgattttttc cttcaacgct
   107941 tcgattgcct gtgcgccacg tgccgcttgt tcagcattga tggattcaat gaccagcacc
   108001 gctgcttgtg tttcttccgt cagcatcgtt ctttgggctt cacgccagtt gagacagcga
   108061 cagatcgctc cttgttcatc atagtagatg atttcttccg gaagtgccgg agcatcggaa
   108121 tctgcaccta aaggcaggaa tgattcgcca ccttgggctt ttcctaaatg cagatttcct
   108181 gcgatctttt cgatatcttc tccgccgcaa ggtacgccaa aagacaagga aatactatta
   108241 tagatatcta caagcggatt gattgggaaa aattcccgtt cttgatttgc acgtttcagt
   108301 aaagcttcaa tagaagaacg tgcacctttt ttagtcttga atgtggtaaa tgcctgacgc
   108361 cactgagcaa tgacttcgtt ttggctgaat gtttcttctg taaggtaatt tcttgcttct
   108421 tttgctgcct cattcaataa ttgatgatac gtttcttcac tttcttttgt tacgtggtta
   108481 tcgataccat ttacggataa gatttcgatg gttgcttcag gaaatacttc ccagaaattg
   108541 gtatccacta ttactttttt catctgttca cacctcttga ttagaataat tgcttgatga
   108601 atagatagtc tgtttgggga tcatctccca tataaaaaac atgttcgctg tatttgataa
   108661 atcctttttt acgataaaaa gcgagcgcaa catcgttgtg ttcccaaaca ccgagccaaa
   108721 tggctgtttt ttgcttttct tttgcttttt caatagctgt atctagtaaa aatgtaccga
   108781 taccttgatg tttaaatttt tttcgtacat agattcgctc gatttctaaa aaatgatcag
   108841 ccatttcctc agattgactc gttcctgtat ttagtttcag gtaacccgca ggctcttgat
   108901 cgatactcac taggaaaaaa tcggtttctt tatcttttag ctcttctgta agtttttcta
   108961 gtgcataagc ttgtgtcaag taatcagcta aatgctccgg tgaattatct tttccaaatg
   109021 tatctgtaaa tgtttcgata ctaatttgtt gtagtaagtc tacatctgaa ggcgtcattt
   109081 ttcgaatttc gtatttattc acggcttctt cctttcaata gatcctttta ttgccttttt
   109141 tgacaaaatt ccaatcttcg ctaacattct tgcatacttt atttaaaaga tattccaatt
   109201 gttttgcttc ttcgtcagaa agaccattta atgcgactgc attggagtag ttgttttctc
   109261 gaatgatgaa aggatagatt tctgctcctt ttttagtcgg atagagtttc ttgatttttt
   109321 tattttcttt atcttccaaa cgttcgatca tcccattgga ttctaatttc ttgattgctc
   109381 gggcagtcgt tgtacgatct actttgatca tttctgctag tttttcttgg atgattccag
   109441 gattttcgca aatacgtact aaataaagat actgtcctct tgttaaatcg acttctttga
   109501 attcgatatt gctaatagaa tctagcgctc gggcaatcat tccgatgtct cgaaggattt
   109561 cagccatttc atcccacctt tattttgttt gtaagactag ttcacctctt tttgttgcaa
   109621 atgcaactat tttcaataaa aaaatcaagt tcttttttaa taactagtaa agtatattct
   109681 tttcttcttt tacgaaaaag ctcaatcaca atagatctgg tttttgtttt ctaaaataag
   109741 taactgtttc ttcgttgttc gcttttctga tagctttggc aactcttcta tactaaaaaa
   109801 tcccatgttg gaagtttcat tattctcaat aaacttggcc tcttcaccgt ggatcgcaca
   109861 agcaaaaaca agtttataat actgaaaaag ctgtggaata tctttttgtt tattcgtatc
   109921 aaaaactgct cgcaactgag tagtttctac tgttaatcct gtttcttcat agacttcttt
   109981 acgcacattt tcttcaggag acaatcctat ttcagcaaat ccaccaggaa gtgaccattc
   110041 tttcgtccgt ttatcttcga ttagtaggac cttgccatct tttttgatat aggcacgaac
   110101 atctactttt ggtgttggat gtccttctcc aatcgttgtg agcgctttta tttcttcgga
   110161 agtttctttt cctatttcac tgatcagttc caatgaaatc gtccggatct cctcaaaacg
   110221 ctctttatca aatacatctt ttccataata tagaccagct tgagaaagtg cgagtagtct
   110281 tttgtattga gccaacttct tgttcattct cttcccttct tttttagcaa atgtatgact
   110341 atgtaatcaa gtatagaaga ctggtttaat tacggcaaat aaatataatg aaatctgcaa
   110401 taaataaaag aatgttaaaa aactcgagaa caaaagacat tcgacttctc tggggctgtg
   110461 aataaatggc gttcaaaagc ttgcatctgc ggtaataagc cccaccaaac gaaaaatatg
   110521 acgagctatt cttgtctggc tgttcttatt aattgaggca ggacagcttt ttcacaacct
   110581 ccccactaaa cggtgttctc ctaccaactc ctcggtcaca agtcacaata ttcatcaaac
   110641 atgagaagct gtttttttga aattgctcct tgtgactcgg agctgaacga caggctcacg
   110701 acctctcggt aaacagcgtt caaaagccaa tttttcgata ttaagccatt tctttgttac
   110761 ttattgtcat tgatttcttt ttctaattta tccaatagtg gagtgtaatc cacttgagta
   110821 tagtctgtta catcaaattc atatagtgtt cctaactgga atttgtcata ttttcggtcg
   110881 gcgatgatct ttttgaatgc ttctttcgta atatgattgg tccctttcgt tcgatcttcc
   110941 aatacgtcac catagtgata atgatccatg atatagctca aatgacgatc tgattcacga
   111001 tcacgtttgt accgacgttg ccataacact tcaaagtctg ctgttaggcg aatcgtgatc
   111061 acctgatatc cgtatctatc tgctaattct tggagtcgtg ctttttgctt atcactaaaa
   111121 ggatactcgg acacaatcac tttctttcct gcttccatat acaaatcaag tgctttgtaa
   111181 tagaaaggat aaactttcgt cctttcttgt tccgcccgtt cttccaaatt gttaaacccg
   111241 atggattctg catagtattc cttgatttca tcgagcgtga tggcatacat atctggaaat
   111301 cgttcccgta gtttattcat aagatatgtt tttcctgtac caggactacc agccagaaga
   111361 atgaaatact ttttcatctc atttatttcc taagttaatt ggactttcca agttcataaa
   111421 cagtccaatc ttatgcttga caatttcttt tactgcttca tttgctggaa tcacattatg
   111481 gcgaagagac ttgttgattt ctgtttcact aaaggtttct ttagctactt tcgtccagtt
   111541 gactaaaagc tccgttccaa cattgaattt gcggatcccg ttattgatca attcaggata
   111601 atcttcttct ttcacacctg ttcctccatg gataactagg gggacatcaa ctactgcatc
   111661 gatttctttc agcaagggaa tgtttacttc tgtttttgat ttgaattgtc catgattcgt
   111721 accgatagca atagctaatg catccacgcc tgtagcttct acaaatttca ctgcgtcttc
   111781 cggtcttgta tataccttgt cattttcagc gacatggatt ccttcttctg taccgccgat
   111841 tgttccaagt tctccttcta cggaaacacc gtgcgcatgg gcatattcta ctactgcttt
   111901 tgtcttcagg atattttctt tgaaaggcag atgggacccg tcatacatga cagaagtata
   111961 tccgctgtcg attgcttctt tgatatcatc aaagtctcgg gcgtggtcta agtgcaagac
   112021 tgcatcaacc atttcttctt ctgcaatagc tgtcattaca ttgactaaca catgcatgcc
   112081 gatatattta gcggtatcga cacttgtttg aataatgatc ggcgaaccca tttcttttgc
   112141 tgcgcctagc atagcaggaa gcatttctag gttatgcgta ttaaaagctc caactgtaaa
   112201 attcaactct tctgctgttt ttgttacttc tttcaatgtt gtatacatct tttattgcca
   112261 ccttatcttt ttatttgttt cctttgatcg ttacttgttt tgcaatttgt cgcatctcat
   112321 ccattttgcc catgtagtaa tcgatccctt gatcatcacc agcacgagca gcatctgcac
   112381 gcttctggtt atagaggctc atcagttttt tatacccatc tccgatcgtc tgtttatttt
   112441 ccaaactctt ttctaaatat tggatggctt gatcgttcac atacaattca gaaaatttaa
   112501 gtccaacttg ttcataaagt ttcgctaatt tatgcttatc ttgggtagtg gaaatctctt
   112561 gtttcaattg tgtaatctct gattgaatct tttcggtcgc ttctggcgat agggcttttt
   112621 cttcttttgc cagaactttt ttcggttctt tttctttttt cttaaaaaat ccaaacatca
   112681 ttttccctac tttcaattaa aatgctttca aaactgggct taagatccag gcatataaga
   112741 gtgctgcggc tactgtatct acatcggttg ctgtagcatt gttgaatccc atcgaattaa
   112801 agatcgttac tagcaaagcg ggcaataatg taataaagaa accatgagca ataccgccaa
   112861 tgatcgctcc tcggcgtccg ccaactgcgt tgccaaatat ccctgctgtc cctccggcaa
   112921 agaaattggt tagcatacca ggtaaaatca tcgctaagcc gaacatgggc aaagtaaaca
   112981 tggcaataat cgtaccaatc gttgtcgtaa tgaaacctaa aatgacagca tttgggctat
   113041 aaggaaagaa aactggacaa tctaaggcag ggatcgcatc aggaactaat ttcatagcta
   113101 tccctcgaaa tgccggcacg atttcaccta acagtagacg tacaccagct agcaatacat
   113161 acacacccac tacgaattgg atggcttgta aaaaggcaaa catgatatag ttttgatctc
   113221 ctgataattc agaagcgaat ggttcgcctg caaataaaac agtgatgata tatagcggga
   113281 ccatcacgac catgacagac aaataagtat cttgcagaaa ttcgaaatgt gtcggcaact
   113341 tgatatcttc gatcgacttt ttattttcgc ctttctcacc aaaaagctta gcaacaccgg
   113401 cttcaaataa gtacccgatc gtacaaaaat gacctaaagc aatatcattt gatccagtga
   113461 ttttacgaat gatcggttgc gctactgccg gcatagcaat ggcgaaacaa gctccgataa
   113521 aaccaccgac caagatcaat acgatccctc gcagaccggc aaaatagcca aagactgtcg
   113581 tcatcgttgc catccataaa attgcttgac cagttaaaaa aatgtacttc cattttgtga
   113641 aacgagccaa aataatattg aatacaaaaa tggctaagaa cgtcagtgca atatctctac
   113701 ctaaacccaa atcattcatt gcttgaccat tgatagattc aatagaaggg atgatccctt
   113761 gcatatgaaa tccttcagta aagattttcc cgaaataagt taaacttcca acgatgatgc
   113821 tcgaaccggc actcaacact tgaaatccta ggagtgtctt taacgtgcca gagatgactt
   113881 gtcctgctga tttcttctgc agtagtagac ccaacatagc aatcaatgca atggtgatcg
   113941 atgcctgagt caaaatattt tcgatgataa aattgataac gctcatgttt ttaccctctt
   114001 ttctttttgt atttagataa ctcccttatc ttgcagaact ggggtgattt tttcttcaat
   114061 ttcatttttt gaaacaatat ttttcaaata gatcatcgcc gttttaggat caactgcgaa
   114121 cttttcgaac tgcgaacgaa aattttctgc tgtgatgatc acatctggtt tcatggaaac
   114181 tgcggaagaa atatcacaat ggtccagttt ggcttctact cctaatttgt tcaaaacatc
   114241 ctcagcagac atctgtgctg caaaactact tcctaaacct gcgccgcata cgaataaaat
   114301 gttcaatttg ttcatctttg ttctcccccg tatcatttat tatttctcaa ctactgaaaa
   114361 aagcagttgt ttgaaatctt cgatatttgt gcattcaatc aatcgatcca acttctcttc
   114421 gttgttgatc aattcaatta gttctttcat gatgttcaga tgggagtagg agtctactgc
   114481 tgccaaacaa aaaataatct ttactggatc catttcagga ttaccgaaat cgattggttg
   114541 ctcaatcgta gctacgctga ctcctaattt attgacccca tcttctggtc gggcatgggc
   114601 tagtgccatg tgtttgccaa tcacgatata cggtccgtaa tcattcactg cacgaatcat
   114661 cgcttcgaca tagcgctctt cgacaatgtt ttctttaacc aatggtctag cagctagttc
   114721 aatcgattcc tcccaagtag tggctttttc tttgatcatg atattgctgt ccgtcaaaat
   114781 atcttttaac ataggctgga tctccctttt gttgatttct aaattgtttg tttcgaataa
   114841 tttttctaac ttgccataga tgattcgatc tactcggcca ccactttctt tcatgacatc
   114901 tattatttga gtgaataact ccgtcgattc atgactgtta ggctctaacc gctgtagttc
   114961 ttcatttctt ttcaaaaagt cttctataac caagatattc gtctcagtta aaatcggatc
   115021 gacaactaaa agcggcttat ttcgataatt caattgaaag gtgctgaaaa ccagattgac
   115081 atccaattta tctactaatt cgatttcttt tgaacttaaa actgcagtaa tctcgatctg
   115141 aggaaacttt tctttcagat tctctgctaa cagatttcca gtagccatcc catggttaca
   115201 gacaacgact gatttataaa cataattcaa gtctcgcttg attacactgg cgatcgtcga
   115261 aaagtggatg acaagaaaag ctatctcgtc ctccagtatc ttgccaccaa ttacttcttc
   115321 gatcgtagga acaaaatgag tgactgcgcg gtagattggg aaataatttg ttcggatgtt
   115381 ttctttcaat ggattcatga cttggatatg attcttgatt cttacaatca gtgcagccat
   115441 gtgcttatag agattttcga ataaggcttc ttcctttaga tggaaaggta cgtgtgtctc
   115501 gttctctaca aattggatca attggatcgt tagtaattga gcttgtaccc actcgattga
   115561 attggagata tctctagcat tgaacgtctc aatagtgaaa cgaagataat ttctttctac
   115621 ttgcgggaca ttgtccaatt gaaattcctc gatcatgtgg tcaataaatg cagaaaagct
   115681 actgtctttt atttctgtat ttttccaatt aatggcagag ataaattcat gtttcctgat
   115741 tcgctggatc cagatcaatg taaaaagcag gatttgtttt ttataaatat catcttcttg
   115801 tttcaacaac ttattggaat agatctcctg tatttttttg atttcctcca aaggaaaata
   115861 atggagaaag attttttgag ccggtgttaa ttgtgcatct tgattcataa agaagtcggt
   115921 cattcccaga ttctgattga taaaatcata gatcatcgta cggatagatc gttctgaacc
   115981 tttataaacc agtccttgct ttccataact gacgatctct atcccataac tgaccagtgt
   116041 tgctcgtaac cgtctcatat cttcatctaa tgtactttta gagatttgaa attcttcttc
   116101 ttttttgttt agatggatcg ggattcggct gtaagcaacg gctaaaacta gatcaaaaat
   116161 acgttcgcgc cgattcaaaa agccgtcttt cacatctttt atttcagttt ctaatcgagc
   116221 ttgcttttcc ttttccagct tcaacaaaaa acctttgttg cgaattgtac ggatctccgg
   116281 aagcttttta ctgctcaacc agatattcgc ttcaagaact tcctgacgaa tcgtccgctg
   116341 actgacattg aactcacttg ctagatgttt tgcttttact ggatattggg ctgtcatgat
   116401 tctgtgaagt atttcaattg ttcttgtttt catagaaatg atctccttct gatttcaagt
   116461 atatggttcc gcttacataa taaaaagtag atatttctgc cacttgaagt ggaaagaaag
   116521 taagcaaagc aaacaaaaaa taagccatat gctttttcat ctcctcaacg aaaaagacat
   116581 acgcctatta ttacaaaaat tttgcccatc tatacaaaaa agaggttgtg aaaaagctgt
   116641 cctgcatcaa gtaataagaa caactaaact aaaatagtgc ttcctatttt ttgtttggtt
   116701 gttcttatta tcgcagatgc aagcttttaa acaccattta gtggagaagc tgtgacagag
   116761 atgttcagcc ttgagcgata agaagcgatt ttgaaaaatc gcttcccata ttttacaaaa
   116821 tcgtgcctta ttgccgatag gctatctctg gaacaccgtt tattcagaaa aaggagctgc
   116881 gacaaggctt ttgtcacaac tccgttcata aatatttcct gtcaatccag cataatcggt
   116941 tgaataaaat cggcttgcca tcttttttct ttctcagaaa aatcaaaata ccatatctga
   117001 gaaaaatact gtcgttgtgt cttacttcct gagatcaatc gcccatcttt tcgatcgact
   117061 gaataatcac agcatctgaa atcaattcgg atggcaaaac tattttcgtg gatctgtcga
   117121 taatttccta cttcctgaat agttattttt ttagtatgat ttcttacacc gtctgctttg
   117181 tttttttgca gaactttctg atgttcggca aacagcttgt ctgtataaca gcttctggct
   117241 ttttcaggag tttcttcttc ccaagcggat tggatcttca tgaatatctt ttggatttcc
   117301 aaaaactgtg ctttcttttc tttagccgtt cctgacatgc gggacaatat ctccgatttc
   117361 agccttttct tttgtctttt attcttgacg atagaaaaag cagaaacccc tcctactgac
   117421 aaaacagcta atgcaagaaa ccccgtttgc tcataactag aaggacctgt acttggacca
   117481 taatacccat aagaatctct gccgtaagaa cgactgccaa aataactgcc cccatatgaa
   117541 cgaccgccaa atgaacctct gcccgaactt cgaccgctag atgtacctcc accatgacca
   117601 cccgcgacag cgaatccttg ggtcggcaga gcaaacaaac ataggcccac taacaaaatg
   117661 attgctaaat aattgatttt ttcattgtct tcctcgttat cttttattac aaaaaactat
   117721 ttttatcata gccttgctaa aaggaagagt caataaccag atgcataaac tgttttttta
   117781 gcaattttgt cttatttagt cattgagtaa catcggacag atgtatgtgt cggcagtcca
   117841 taattcctct atcttcagca tgaacaagct tgcagacaag agatctgcgc ttcctccagg
   117901 agagatttgg ttttcctgac aaaagtgcaa cagtgctgta aatggacgat ttggtaaagc
   117961 actttgacta gcagcatgag caagtttttg catttccact aacgttttct gatcactgcg
   118021 atgcaatacg catgtgtcat ctagcgaact catcaaaact aacaacatat gcatttgttt
   118081 ctgtacatat gaatcattat ctagctcctt agctgcttgg attgcaagcc tgatatgcgg
   118141 ataccctaat atcgcttctt catatgcacc atttacgcca tatttttgct tcaccctatg
   118201 accatgagtg gtttttttat gacgatattt tgtatcagga aaagcagcaa gcttgctgac
   118261 atcatcaagt attttcgata aagaaaaagg ctgttccata ctgatttttt gtgcaatgac
   118321 actgatcaat agccccattg cccaaatcgc tcctttatgg gtattgatgc catttgtagc
   118381 aatcagcatt gccttttccg cttttcgccc aatctcagca attctttctc ttaactcctg
   118441 atcaatagca taatgccatg ctatttgagc catttcttca aaaaaaggag ttagcgtctt
   118501 agcagactta tccaaaatgc gccgaccttc ttttacggct tttgcgatcg tatggaaatt
   118561 atcatctcct aaaaccatat cagctgcctg tttgcttact tcactacctt tgattcccat
   118621 agcgattcct atatcggctc ttttcaaagc aggtgcatca tttacgccgt ctccggtcat
   118681 tccgacgatt tctccatttt tctgcaaagc tttcacgatt cgcagtttgt gttctggtgt
   118741 cgttcgagca aagacgtcga cttttggtac ttgtcgaatc aactcttctt cagacataag
   118801 atccaaatcg atcccttcta aaactttagc agtatgtttt aagccaattt gttccgcgat
   118861 cgcttttgct gtatctttgt gatcccccgt aatcattttc actgagattc ctgcttgttg
   118921 gcattccttt acagctttga ctgcactttc ttttggcgga tcaatcaacc cagcaatccc
   118981 tgcaaaagtt aattgggtta aagactcatg cgtgagttcc tgtttcgaat ctactttttt
   119041 gtaagcaaat cctaaaacac gctgaccttt tttcgcaaac gctgtggctg tttgacccca
   119101 tgcttcttat tctgaagaag aaagagttga tttagccaat aatacttctg gcgcaccttt
   119161 gacgtaaatc acccctcctt tttcctccgc atgcatcgtt gccatatatt tgtattcaga
   119221 gctgaacgga atttttgctt ctattttttt caaaggcaga tcggctgttt cggtgtggcg
   119281 aatcagtgca agttctgtcg gattgccact taaattttct gttttctgtt cttctttcgt
   119341 ctttaattcc tgacaatttt tcataataaa cagtacgcga gtttcttcct ttgctgctac
   119401 atccacaact gtcatttcat tttttgtcaa tgtccctgtc ttatctgaac agatcactgt
   119461 catagaacct aatgtttcta cagaaggcag tcctttgatg atcgcctttt cttttgccat
   119521 ttcatgtact ccgacagata ggatcatggt caacacagct ggcaatcctt cgggtaccat
   119581 tgacaccacc aaagcgatca tagctgaaag aaggatatgc caatccatgc catagcggaa
   119641 tgttgtaaaa acaactaaga acaagatcaa acaaagaatg ccttgaaaaa tttgtttgtt
   119701 caatcggttg atcttcttga tcagaggcgt cgtttgttgt tcgaccgatt gcagtgcttg
   119761 attgatttta ccgatttctg tcgtgtttcc tgtttctaca acgacaccga ctgcagaacc
   119821 agattgtaca agcgttcctg aaaaagccat atttttttgg tcaccgattg attcttctgc
   119881 tgccaaaacg gaatgatttt tttcagatgc ttctgattct cccgtcaaaa tcgattcttc
   119941 tactagtaga tcatgtactt cgatcaaccg aacatctgct ggaacgacgt ctccggcacg
   120001 taagacaatg acatctcctt ttactagttc tttcgcaggg attttttctt gcatcccgtt
   120061 cgatagtact atcgcttctt gtcccatcag ctgcttcaaa ccattcaacg attcttctgc
   120121 ttttctttct tgccaataac taacgagtcc gttgacgatc actacgaata aaataatact
   120181 tccttcgatg tattcgctcg aagcaaattt caaaaaagag gcaatgatca acacgatcat
   120241 cagcagatca gtaaagtgtt ttgctatttt tttccatcgt tttgtctgct gtcttgcttc
   120301 tatctcatta tatccttgat cagtcaggcg tttctgccgt tcttcttgcg acaagccttc
   120361 tgttgatgtt tgatagtttt tcaatacatc ttctaccgtc aatctgtacc agttcatcct
   120421 atcccctcct gtttaattga tactgttatt atacgcaggg aagttttttc aggcgtatcg
   120481 attatttcag gggggtctga aataattcgt tttgcagaat gtatctgggc ctaactgata
   120541 tttctattgc ttattctttt atatacaaaa tttaagaata caaaccagat aaccaataaa
   120601 aaaggaataa agcgtgtcgt gctttcgaat aacaagctga aagcaattga agtaaaaaac
   120661 accaacaaag caagatcttt atatctttgt gtagtgttcc gtttcttttc tttaataaat
   120721 tgaacatgtg cacacaagat cgaagaccac acaaatagaa aacaactcgt tgtcatgcct
   120781 gccaaaagct gaaaaaacaa tcgactatca gctacaaaaa aatgtagaca aagaccgaca
   120841 aaaaataaca tccctgtaat taaaataccg tgtaaaggta cgcctttctt agaaagcgca
   120901 caaaaccaat ggggcaaact tttctctttt gctgtttgat ataaaatcct gctagtactg
   120961 tagactgcac tatttgctga ggacaaagaa gcaaccaaaa tcattagatt gacaattgct
   121021 gctccgcttt ggcaatggat cacttgcagc atagtcacta aaggactttc ttttaaatct
   121081 aaacaatgcc atggaacaac aaataaaatc gacaaaatcg ttcctatata gaaaaagagg
   121141 atacgccaag gcagacagtt gattgcattt ttcaaattct tttgtgggtc actcgtctca
   121201 ccaactgtta atccgatcat ttcaattcca gcaaaactga ataaaaccat aggaaatgaa
   121261 gcaatcattc cagcaaagcc aaacgggact aattcattga gctgtccaaa atttgctttg
   121321 attccaatag actgggtatg aaaatctgag acactcaatc ctatacccaa taagataaaa
   121381 aggaaaatcc ccaagatctt gatgattgag aaaaaagatt ccaattcacc aaaccacttg
   121441 actgcaacaa ggttgactgc cattaagatc attagtgcgc tacctccgct tatcaaagct
   121501 ggtacatgta caaaccagaa atggatatac aatcctattg ctgttaattc cgcaagtcca
   121561 gctccaagcc agcaaagcca ataagtccaa ttgacaaaaa atgcccactt tttgcctaaa
   121621 taatagtcaa cgaaatcact gatagagtta aatgtactat ttgctaaaaa taaatcaccc
   121681 atgacccgca tcaaaccata aatagccaaa ccgcatatca aataaacgag aataacagct
   121741 ggccctgctg atgcaattgc ctttgctgaa cctaaaaata atcctgttcc aattgcgcca
   121801 cctaatgcga ttaactggat atttcgatta gttagagtcc gtttcattgc cggtttttcc
   121861 atcaagttca cctttttctt tttcaagcag catggcgact tcctgttccc aagaaggttt
   121921 ctttattttt tcaatacaga gtggtactac aaacaaaatg acactgatgg ctgtgactgc
   121981 aattagattg atccatgtcc cagctttcat cgtcagtccg acagaaaatc cgattgccgc
   122041 aaataaaaca ataacgacag tccaaactcc aagattgctc tttttctctt tttttccaat
   122101 tctaaatggt cgattcaact ttgcatgtgt ataacgcaat ctcaaaactg cgatccccat
   122161 gatgacatag acgatacagt agataacagt agttgaattt acgagcatca aaaatgcttg
   122221 attgacgcca ggaatcagca aataaaaaag tgcaaagatt gagataatga cagactgagt
   122281 caaaataacg gatttggata cgtcaaattg attcgtcttc caaaaattga attttggtgg
   122341 atacgcacct tttctagcac tttctgtcac tgtctttgct ggtcctgagg cccaagcaga
   122401 taattggact gctacaccaa tgaagacaag taaactaaac agattcacaa taagatgtgg
   122461 caaaccaaga atttggcaat aaatcgaaat agattgcgcg atattattca attccatttg
   122521 tccttttggt acgacatttg ctacaactaa agcattcaaa atattgacga aaaatataaa
   122581 aattaacgcg gcaaatactc cgcgaatata cgttttaact ggatttttca accgagtgat
   122641 atacacagag gacatttcga taccagtaaa aataaacatg atcggcgcaa aataaacaaa
   122701 tgaggatgca gtcgtagtat ctggtattaa tttgtcccat gaaaaactgc ctaatgttcc
   122761 ggttggtacg atcccaactt tgatccacgc agcaaaccct aataagagca tcatcgtcaa
   122821 aggcacgtac aatcctaacc aaacaccgac ttttccgccg atttttgcca tatcaaattt
   122881 caaattcaaa aaagtgatga tccaataaac gactaaaatg acaactaatg taaatttgct
   122941 gttcaaacca agcggcacat tatcgataat attaccaaac aacggagcaa gtgctgaagc
   123001 gaccatcacc atcccaggaa acatttggac ccataacagc catgagacaa cgaaccccca
   123061 tttttttcct agagcattcg atacccaaag ttctggacca cctttttggg gaaacattcc
   123121 ggcaaattca ccagagatca atgagattgg aaaagcgtac aataatactg caaccagcat
   123181 gtaaaaaatc atcgtccagc tcgttgctgc tacatctgga atatttcgta cacttgctac
   123241 aagggcgcag gtcattccga taaaggtcat ggtcgtgatt tgctttgatg aaccagtatc
   123301 tttcatcttt attccttctt tcaatagcga catatgtttt tgttttcttt tatttttgtt
   123361 caagaacaga aatgacaagg tttcatcgat tcagtacaaa acccagtatc accgaaacat
   123421 ccattgaacg atcttagtga actgggtttg atacatgaaa cgaacgatcc aacttataga
   123481 acgttttata aaaaagaata tcttggcgag gaatatatga aaaacagggg gaaatacaat
   123541 tgttttgatg aagcaaataa ttgtctgttc tccatatgtc tcttgttatt ctttcacaca
   123601 aaaatcagtt ctattggatg atttcgttta gtttcttttc cattgctttg gcgatttctt
   123661 ttgtataaaa attgaaaatt cgatcatcat tcaaataagg agtcatgata gccgcacgta
   123721 gcagagtgat tttctgttct tttttccatt cttctttcgt tagacccatt ctttcaacaa
   123781 aacgaatcgg agaatcaccg taatcttctt gtgtaaatgt agtatgagaa gtaataaacc
   123841 gttcaccata aacatcgcca tccatataag aagaccgatc gaacattttt tcatttaatt
   123901 cattgatagc gttcaaatct gtacagcctt gttctttgaa tacccaattg accatattaa
   123961 aatcaggatg attcaatgga tagacttcga ttgtttttcc tttaacagta aaagtcagat
   124021 gatcgagaaa ttctctgaat ctttgagctg cctcgattga agccccaatc aattgaccat
   124081 atccggtcac attcaatggc aatacacgat gtgctgtcca gactgcggcg gcggttgctc
   124141 cagcttttga tccttccaaa atataagctc ctaacatatc tggagccttt actgattttt
   124201 caaacacata tggcgcgaaa taagaaatga tatttctcat atttttatgt ttgattacga
   124261 ttcctccagc agcataaggg acatagccca tcttatgagg atctatcgtg acagaatccg
   124321 cttctgaaat agctctgaac ccttcatata cctctttgtc aatcgttacg cagtggtgga
   124381 aaacatgatg ttcttcaaag aattcagcta aggaagcata cggcacaaat tctcctgctt
   124441 cgtttagaaa cagtgaacgc gcatagccgc catatgcggc atcaacatgt aaatagaaat
   124501 agatcccttc atcttttaac ctttctctta attggacgat tttatctaca ctgtctactt
   124561 gaccttcttc tgtcgttcct acgacagcaa ccacacctag aattgggatt ttttgttcag
   124621 ctaattcacg aattgttttt tccaaagcat taatatccat tcgataatcc tcttgaactg
   124681 ggatagcaac catctgatct aagcctactc cgcaaatatc taacgccttc atccaagaat
   124741 agtgttttgt ttgaggcacc agccatttcc ctaatctttg gatatttttt ccgctacggg
   124801 aagaagctgc tttgacctca tccatttctt cgtccgtaaa gctttcggtc atttccaaaa
   124861 tttcttcaac agataaattc aaaagttccc actcagacat ttttttcact tgttcaggca
   124921 gcacttcctt gacagctaaa ggaatcgatt tgatgcagcg ggcgtaccat aatccttcaa
   124981 gattagcaat agaaccatct gctgcaatgt gtccccatcc gtctgtcata ttgaataatg
   125041 aagcaaaatc ctgtcctact tctgcttcca tttgcgaagt tgccatagaa gattccaatg
   125101 caacattatt tgggttccac agcatcgcat agttgtaagc taataaagca ggcatcaatg
   125161 tttcagcatt catttgtccc cagtaacggc ctgctgaatg ccatgggatc gatcctgcgc
   125221 gtaatcgctg actgacttca tccaagacct ctatcatatg gtcgcgggtg gctagataat
   125281 ctggagtgag cttatctcct tcgctaattg caggcatatc tgaaggaata taattttctc
   125341 tccatccaag atgttcatct accatcttgt tcaacaacat tttataaact ggtccatttt
   125401 cagctttgtc gcctataaag acggccttga tatccatatc tttcattttt cttccctcca
   125461 tattttgttg gtcaatgtct cgcagataca tgtgtttatc tgtaacacct cttcactata
   125521 gcgccaaaaa tgagaaattt ttttttataa catcaaatcc acgtagattt tta
//
LOCUS       DACEUF010000002        91707 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.002, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000002 DACEUF010000000
VERSION     DACEUF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 91707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.002"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            218..736
                     /locus_tag="IXG20_000107"
     CDS             218..736
                     /locus_tag="IXG20_000107"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1608329.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            864..1547
                     /locus_tag="IXG20_000108"
     CDS             864..1547
                     /locus_tag="IXG20_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ1608330.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1856..1929
                     /locus_tag="IXG20_000109"
     tRNA            1856..1929
                     /locus_tag="IXG20_000109"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1890..1892,aa:Arg,seq:tct)
     gene            2088..2447
                     /locus_tag="IXG20_000110"
     CDS             2088..2447
                     /locus_tag="IXG20_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ1608331.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2570..4201
                     /locus_tag="IXG20_000111"
     CDS             2570..4201
                     /locus_tag="IXG20_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ1608332.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4263..4904)
                     /locus_tag="IXG20_000112"
     CDS             complement(4263..4904)
                     /locus_tag="IXG20_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1608333.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            5186..5494
                     /gene="rplU"
                     /locus_tag="IXG20_000113"
     CDS             5186..5494
                     /gene="rplU"
                     /locus_tag="IXG20_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ1608334.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5521..5865
                     /locus_tag="IXG20_000114"
     CDS             5521..5865
                     /locus_tag="IXG20_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ1608335.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5878..6171
                     /gene="rpmA"
                     /locus_tag="IXG20_000115"
     CDS             5878..6171
                     /gene="rpmA"
                     /locus_tag="IXG20_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ1608336.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6578..9412
                     /locus_tag="IXG20_000116"
     CDS             6578..9412
                     /locus_tag="IXG20_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1608337.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            9603..10094
                     /locus_tag="IXG20_000117"
     CDS             9603..10094
                     /locus_tag="IXG20_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ1608338.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10197..11186
                     /gene="manX"
                     /locus_tag="IXG20_000118"
     CDS             10197..11186
                     /gene="manX"
                     /locus_tag="IXG20_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ1608339.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11219..12022
                     /locus_tag="IXG20_000119"
     CDS             11219..12022
                     /locus_tag="IXG20_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ1608340.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            12043..12957
                     /locus_tag="IXG20_000120"
     CDS             12043..12957
                     /locus_tag="IXG20_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ1608341.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            13040..13414
                     /locus_tag="IXG20_000121"
     CDS             13040..13414
                     /locus_tag="IXG20_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ1608342.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13553..13798
                     /locus_tag="IXG20_000122"
     CDS             13553..13798
                     /locus_tag="IXG20_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1608343.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13970..15034
                     /locus_tag="IXG20_000123"
     CDS             13970..15034
                     /locus_tag="IXG20_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1608344.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15280..15705
                     /locus_tag="IXG20_000124"
     CDS             15280..15705
                     /locus_tag="IXG20_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1608345.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15698..16147
                     /gene="nusB"
                     /locus_tag="IXG20_000125"
     CDS             15698..16147
                     /gene="nusB"
                     /locus_tag="IXG20_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ1608346.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16244..17098
                     /locus_tag="IXG20_000126"
     CDS             16244..17098
                     /locus_tag="IXG20_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ1608347.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            17099..18436
                     /locus_tag="IXG20_000127"
     CDS             17099..18436
                     /locus_tag="IXG20_000127"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ1608348.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18441..18665
                     /locus_tag="IXG20_000128"
     CDS             18441..18665
                     /locus_tag="IXG20_000128"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ1608349.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18667..19557
                     /locus_tag="IXG20_000129"
     CDS             18667..19557
                     /locus_tag="IXG20_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1608350.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19566..20384
                     /locus_tag="IXG20_000130"
     CDS             19566..20384
                     /locus_tag="IXG20_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ1608351.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20491..20940
                     /locus_tag="IXG20_000131"
     CDS             20491..20940
                     /locus_tag="IXG20_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1608352.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20958..22640
                     /gene="recN"
                     /locus_tag="IXG20_000132"
     CDS             20958..22640
                     /gene="recN"
                     /locus_tag="IXG20_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ1608353.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22695..22811)
                     /locus_tag="IXG20_000133"
     CDS             complement(22695..22811)
                     /locus_tag="IXG20_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ1608354.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22884..23840)
                     /locus_tag="IXG20_000134"
     CDS             complement(22884..23840)
                     /locus_tag="IXG20_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1608355.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            24011..24379
                     /locus_tag="IXG20_000135"
     CDS             24011..24379
                     /locus_tag="IXG20_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ1608356.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24549..24980
                     /gene="mraZ"
                     /locus_tag="IXG20_000136"
     CDS             24549..24980
                     /gene="mraZ"
                     /locus_tag="IXG20_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ1608357.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            25001..25960
                     /gene="rsmH"
                     /locus_tag="IXG20_000137"
     CDS             25001..25960
                     /gene="rsmH"
                     /locus_tag="IXG20_000137"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ1608358.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIGLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25965..26366
                     /gene="ftsL"
                     /locus_tag="IXG20_000138"
     CDS             25965..26366
                     /gene="ftsL"
                     /locus_tag="IXG20_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1608359.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26367..28559
                     /locus_tag="IXG20_000139"
     CDS             26367..28559
                     /locus_tag="IXG20_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1608360.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28585..29547
                     /locus_tag="IXG20_000140"
     CDS             28585..29547
                     /locus_tag="IXG20_000140"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ1608361.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29561..30922
                     /locus_tag="IXG20_000141"
     CDS             29561..30922
                     /locus_tag="IXG20_000141"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ1608362.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30948..32036
                     /gene="murG"
                     /locus_tag="IXG20_000142"
     CDS             30948..32036
                     /gene="murG"
                     /locus_tag="IXG20_000142"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1608363.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            32051..33271
                     /locus_tag="IXG20_000143"
     CDS             32051..33271
                     /locus_tag="IXG20_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ1608364.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33427..34752
                     /gene="ftsA"
                     /locus_tag="IXG20_000144"
     CDS             33427..34752
                     /gene="ftsA"
                     /locus_tag="IXG20_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ1608365.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34775..36016
                     /gene="ftsZ"
                     /locus_tag="IXG20_000145"
     CDS             34775..36016
                     /gene="ftsZ"
                     /locus_tag="IXG20_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ1608366.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            36033..36710
                     /locus_tag="IXG20_000146"
     CDS             36033..36710
                     /locus_tag="IXG20_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ1608367.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36724..37323
                     /locus_tag="IXG20_000147"
     CDS             36724..37323
                     /locus_tag="IXG20_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ1608368.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37338..37625
                     /locus_tag="IXG20_000148"
     CDS             37338..37625
                     /locus_tag="IXG20_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ1608369.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37674..38456
                     /locus_tag="IXG20_000149"
     CDS             37674..38456
                     /locus_tag="IXG20_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ1608370.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38477..39184
                     /locus_tag="IXG20_000150"
     CDS             38477..39184
                     /locus_tag="IXG20_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ1608371.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39446..42244
                     /gene="ileS"
                     /locus_tag="IXG20_000151"
     CDS             39446..42244
                     /gene="ileS"
                     /locus_tag="IXG20_000151"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ1608372.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42293..43819)
                     /locus_tag="IXG20_000152"
     CDS             complement(42293..43819)
                     /locus_tag="IXG20_000152"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ1608373.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43834..44481)
                     /locus_tag="IXG20_000153"
     CDS             complement(43834..44481)
                     /locus_tag="IXG20_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ1608374.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44665..44994)
                     /locus_tag="IXG20_000154"
     CDS             complement(44665..44994)
                     /locus_tag="IXG20_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ1608375.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45171..45899
                     /gene="sfsA"
                     /locus_tag="IXG20_000155"
     CDS             45171..45899
                     /gene="sfsA"
                     /locus_tag="IXG20_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ1608376.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45915..46928
                     /locus_tag="IXG20_000156"
     CDS             45915..46928
                     /locus_tag="IXG20_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1608377.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46928..48205
                     /locus_tag="IXG20_000157"
     CDS             46928..48205
                     /locus_tag="IXG20_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1608378.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48268..50970
                     /locus_tag="IXG20_000158"
     CDS             48268..50970
                     /locus_tag="IXG20_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1608379.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            51122..51439
                     /locus_tag="IXG20_000159"
     CDS             51122..51439
                     /locus_tag="IXG20_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1608380.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51469..51789
                     /locus_tag="IXG20_000160"
     CDS             51469..51789
                     /locus_tag="IXG20_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1608381.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51897..53357
                     /locus_tag="IXG20_000161"
     CDS             51897..53357
                     /locus_tag="IXG20_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1608382.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53425..53646
                     /locus_tag="IXG20_000162"
     CDS             53425..53646
                     /locus_tag="IXG20_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608383.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53677..53859
                     /locus_tag="IXG20_000163"
     CDS             53677..53859
                     /locus_tag="IXG20_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ1608384.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53859..54272
                     /locus_tag="IXG20_000164"
     CDS             53859..54272
                     /locus_tag="IXG20_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ1608385.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54395..55576
                     /locus_tag="IXG20_000165"
     CDS             54395..55576
                     /locus_tag="IXG20_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1608386.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            55965..56038
                     /locus_tag="IXG20_000166"
     tRNA            55965..56038
                     /locus_tag="IXG20_000166"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55999..56001,aa:Arg,seq:cct)
     gene            complement(56107..57246)
                     /locus_tag="IXG20_000167"
     CDS             complement(56107..57246)
                     /locus_tag="IXG20_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1608387.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(57545..58180)
                     /locus_tag="IXG20_000168"
     CDS             complement(57545..58180)
                     /locus_tag="IXG20_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608388.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58293..58928)
                     /locus_tag="IXG20_000169"
     CDS             complement(58293..58928)
                     /locus_tag="IXG20_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ1608389.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58962..59423)
                     /locus_tag="IXG20_000170"
     CDS             complement(58962..59423)
                     /locus_tag="IXG20_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ1608390.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59553..59984)
                     /locus_tag="IXG20_000171"
     CDS             complement(59553..59984)
                     /locus_tag="IXG20_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1608391.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDK
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(60002..60322)
                     /locus_tag="IXG20_000172"
     CDS             complement(60002..60322)
                     /locus_tag="IXG20_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1608392.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60621..61397
                     /locus_tag="IXG20_000173"
     CDS             60621..61397
                     /locus_tag="IXG20_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1608393.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61412..61615
                     /locus_tag="IXG20_000174"
     CDS             61412..61615
                     /locus_tag="IXG20_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1608394.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61631..61969
                     /locus_tag="IXG20_000175"
     CDS             61631..61969
                     /locus_tag="IXG20_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ1608395.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            61956..62135
                     /locus_tag="IXG20_000176"
     CDS             61956..62135
                     /locus_tag="IXG20_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608396.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(62178..62648)
                     /locus_tag="IXG20_000177"
     CDS             complement(62178..62648)
                     /locus_tag="IXG20_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608397.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            62735..63433
                     /locus_tag="IXG20_000178"
     CDS             62735..63433
                     /locus_tag="IXG20_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ1608398.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            63611..63952
                     /locus_tag="IXG20_000179"
     CDS             63611..63952
                     /locus_tag="IXG20_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608399.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            63945..64616
                     /locus_tag="IXG20_000180"
     CDS             63945..64616
                     /locus_tag="IXG20_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ1608400.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            64622..65308
                     /locus_tag="IXG20_000181"
     CDS             64622..65308
                     /locus_tag="IXG20_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608401.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            65311..66060
                     /locus_tag="IXG20_000182"
     CDS             65311..66060
                     /locus_tag="IXG20_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1608402.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            66072..66341
                     /locus_tag="IXG20_000183"
     CDS             66072..66341
                     /locus_tag="IXG20_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608403.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            66503..66805
                     /locus_tag="IXG20_000184"
     CDS             66503..66805
                     /locus_tag="IXG20_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608404.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            66802..66963
                     /locus_tag="IXG20_000185"
     CDS             66802..66963
                     /locus_tag="IXG20_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1608405.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            66960..67265
                     /locus_tag="IXG20_000186"
     CDS             66960..67265
                     /locus_tag="IXG20_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608406.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            67265..67621
                     /locus_tag="IXG20_000187"
     CDS             67265..67621
                     /locus_tag="IXG20_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ1608407.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            67581..67826
                     /locus_tag="IXG20_000188"
     CDS             67581..67826
                     /locus_tag="IXG20_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608408.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            67823..68242
                     /locus_tag="IXG20_000189"
     CDS             67823..68242
                     /locus_tag="IXG20_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ1608409.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            68239..68796
                     /locus_tag="IXG20_000190"
     CDS             68239..68796
                     /locus_tag="IXG20_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ1608410.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            68793..69089
                     /locus_tag="IXG20_000191"
     CDS             68793..69089
                     /locus_tag="IXG20_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608411.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            69166..69579
                     /locus_tag="IXG20_000192"
     CDS             69166..69579
                     /locus_tag="IXG20_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1608412.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(70037..70312)
                     /locus_tag="IXG20_000193"
     CDS             complement(70037..70312)
                     /locus_tag="IXG20_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608413.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            70766..70972
                     /locus_tag="IXG20_000194"
     CDS             70766..70972
                     /locus_tag="IXG20_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608414.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            71168..71335
                     /locus_tag="IXG20_000195"
     CDS             71168..71335
                     /locus_tag="IXG20_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1608415.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            71361..71705
                     /locus_tag="IXG20_000196"
     CDS             71361..71705
                     /locus_tag="IXG20_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1608416.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            71710..71991
                     /locus_tag="IXG20_000197"
     CDS             71710..71991
                     /locus_tag="IXG20_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608417.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            72094..72408
                     /locus_tag="IXG20_000198"
     CDS             72094..72408
                     /locus_tag="IXG20_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ1608418.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            72386..74080
                     /locus_tag="IXG20_000199"
     CDS             72386..74080
                     /locus_tag="IXG20_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1608419.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            74100..75278
                     /locus_tag="IXG20_000200"
     CDS             74100..75278
                     /locus_tag="IXG20_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1608420.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            75241..75927
                     /locus_tag="IXG20_000201"
     CDS             75241..75927
                     /locus_tag="IXG20_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ1608421.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            75927..77087
                     /locus_tag="IXG20_000202"
     CDS             75927..77087
                     /locus_tag="IXG20_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1608422.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            77097..77972
                     /locus_tag="IXG20_000203"
     CDS             77097..77972
                     /locus_tag="IXG20_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608423.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            77969..78280
                     /locus_tag="IXG20_000204"
     CDS             77969..78280
                     /locus_tag="IXG20_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608424.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            78270..78623
                     /locus_tag="IXG20_000205"
     CDS             78270..78623
                     /locus_tag="IXG20_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1608425.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            78613..79014
                     /locus_tag="IXG20_000206"
     CDS             78613..79014
                     /locus_tag="IXG20_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608426.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            79007..79411
                     /locus_tag="IXG20_000207"
     CDS             79007..79411
                     /locus_tag="IXG20_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608427.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            79423..80031
                     /locus_tag="IXG20_000208"
     CDS             79423..80031
                     /locus_tag="IXG20_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1608428.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            80051..80413
                     /locus_tag="IXG20_000209"
     CDS             80051..80413
                     /locus_tag="IXG20_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608429.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            80416..80598
                     /locus_tag="IXG20_000210"
     CDS             80416..80598
                     /locus_tag="IXG20_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608430.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            80615..84046
                     /locus_tag="IXG20_000211"
     CDS             80615..84046
                     /locus_tag="IXG20_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1608431.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVKKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            84097..84834
                     /locus_tag="IXG20_000212"
     CDS             84097..84834
                     /locus_tag="IXG20_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1608432.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            84844..87135
                     /locus_tag="IXG20_000213"
     CDS             84844..87135
                     /locus_tag="IXG20_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608433.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            87159..89285
                     /locus_tag="IXG20_000214"
     CDS             87159..89285
                     /locus_tag="IXG20_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608434.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            89448..89894
                     /locus_tag="IXG20_000215"
     CDS             89448..89894
                     /locus_tag="IXG20_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608435.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            89896..90033
                     /locus_tag="IXG20_000216"
     CDS             89896..90033
                     /locus_tag="IXG20_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1608436.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            90071..90364
                     /locus_tag="IXG20_000217"
     CDS             90071..90364
                     /locus_tag="IXG20_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608437.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            90361..90585
                     /locus_tag="IXG20_000218"
     CDS             90361..90585
                     /locus_tag="IXG20_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1608438.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            90582..91607
                     /locus_tag="IXG20_000219"
     CDS             90582..91607
                     /locus_tag="IXG20_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1608439.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNPYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT        31039 a        14935 c        19281 g        26452 t
ORIGIN      
        1 agacataaag gtattccttt ctaaaacttg taatgttagg gtaaacgttt tttaaactaa
       61 cgtcaattat ttataataaa aaatgagata aagatgagga aatagaatta gaaacatata
      121 attattaaag ggaaacattt ttacagctat aatttgaaaa aaatttatcg ttattatatg
      181 attaagaagg attgaataaa gaaagaagtt gaaaacgata cctgattaca aaaaaataac
      241 ttttataagt tctataacgt tgattatatt tttaattctt attcttctcg tcgtcttggt
      301 tccccgaatt tttggtttga ctccttatat tattgaggac aaagaaatga gtccatatta
      361 tgcacaagga aatctgatat atgtacgacc gaaaaataca aatgagattt tagtcggaga
      421 tattattaca tattatgaaa attcaggaaa aaaaatctcg acgcgtcgcg taatagccgt
      481 tgaaaacaac cacgaagatt tttatacaaa agctgataca aagacttata ttgagcctgg
      541 agtagtaaaa aagagaaata ttatcggtag tcccatcttt cagattccat atattggact
      601 agtcctttca aaaacggcat ttgcatggat tcaggggtta ttctttacga ttgctttttg
      661 cctaacagga atcacagcat ggaatacctt tgtagagttg aaaaagaagc ggagacgaac
      721 acagaaattt ttgtaagaat gttaaaaaca taaatgtatt ccagagatcc aacgactctt
      781 ctgaacatga aacacgagaa aacactcatg cagcttgtag atttagtcag ttgttcgtat
      841 aatgtgtgaa gaggaggagt agtatgcgca atgaaatgat gcatcgtcct gtagtcaaag
      901 aagaactatt ggattttatg cgtacccagc aaaaaaaatt accgggtgag ctaggcaaac
      961 tggaagaaga agctcatgta gcagaagtac cagttattcc ccatgaaaca gttgtatttt
     1021 tgaaattttt acttggacag cttcagcctg aacgaatctt ggagatcgga gcagctatcg
     1081 gtttttcatc aagtgtcatg gccacgacaa tcggagaaaa cgctcatgtt acgacaattg
     1141 atcgtttcga tgtcatgatc gagaaagcta aaaaaaacta tgaacgtcta ggactgacag
     1201 ataaagtgac tttgcttgaa ggtcaagcag cagatatctt gccggagttg tctggtccgt
     1261 atgactttat ttttatggat agtgcaaaat caaaatatat tgaattttta cctgaatgct
     1321 tacgtttgtt aagaaaaggt ggcgttttga tggtagatga catcttccaa ggaggaacga
     1381 tcttgctccc agatgaagag attccccgtg gcaaacgagc tatccatcgt aaattaaatg
     1441 aatttcttcg tgttgtgatg gatcatcccg atttgacatc gacaatcttg ccattaggtg
     1501 atggtgtgat cttaatgaca aaggaatcag aaacaatcga gttgtgatac atgattatgg
     1561 cagcctgtag ataatcgatt gaatcgaggg ctcaattcga acttgatgta aaagtttcta
     1621 tatttgtaag tcaattaaag gatacaggaa atctaaataa acaggagtag catgaagtca
     1681 atgtagctcc tgttttcaat tgataacctt agaacagctg aaggagaatg tctcgtttta
     1741 aaagattgat ttagtagttg ggccaaaata attcattatt tgctaattaa ataactattt
     1801 cgtattgaca gaagcaattt aattatctat aattattgat gttccgctga ttcttgtcat
     1861 agtagctcag ctggatagag catccgcctt ctaagcggac ggtcgggggt tcgaatccct
     1921 cctgtgacgc taaaaaaccg ttagaaactg aggtttctaa cggtttttct ttgttttttg
     1981 tgccaatatt gtttgttttc aaaggcaaat aaaataaaaa aacatcctta tcgagtacaa
     2041 ttataaggag caaaaaaata aaactcatca gaaaagagga agaagatatg acgatactat
     2101 ctagaatttt agtgactcta gttgcaattg aattttttta tatcatgtat atcgaaacgg
     2161 tgagaactga ttctgacacg acaagtaggg tttttaaaat gagtaaagaa gagttaagca
     2221 gaaaatctgt tcaaacattg tttaagaacc aaggagtgta caatggacta ttaggagtgg
     2281 gactacttta tggagcttat ctttcttccg catctaaaga aatcacaagt atgctattaa
     2341 tatctatttt ttttgttgca ttatatggca gtttgactag cgatcgttgg attttactca
     2401 aacaaggtgg gctagctctg ttggcattgc tttctatttt cttataaata tagaaaaaat
     2461 aagaatttat ctaacaggaa actttgtgaa aaatattact ttatctcatg aaatatgata
     2521 ttctaatgaa tggtatttta gaaaatttca tgaaaaagga gttagatagg tgttcaaaaa
     2581 ttatgtttcc tcactaaaaa aagaattttc aggttatgat ctttcaagat ttcagaaaga
     2641 tcttttggct gggatcacag tagctgctgt agcattgcct ttagccttgg cttttggggt
     2701 atcaagcgga gctgatgcgg ccgcaggttt gattacggct atcattgccg gtatcgttat
     2761 tggcggttta tcaggcggtt tttatcaaat atcaggtccg acaggtgcga tggcagctat
     2821 cttgatgtct attgcggcaa caaagggaat gcaaggtgta ttgcttgcta cttttttagc
     2881 gggtctattt ttacttgcag caggactttt ccgattagga acactgactt cttttatccc
     2941 agcacccgta attacggggt ttacttcagg tattgctatc attattgcat taggtcagat
     3001 cgataattta tttggtgttc actcggaagg tacaaacgtt attgaaaaac taagtagtta
     3061 tcaaacgata ggatttccga taaacacagc ctctttgatc atgggagggc tagtcatttt
     3121 agggatgttt atctatccaa aaaaatgggc gaaacgaatg cctagttcgc tgctggctat
     3181 cattttggca acagcagtta tgctattgtt ccatttacca gtagcaactg tgggtaagat
     3241 tccacaaaca ttgatcagta gtaatcgttt gaatatggga gattttagct tttctgcctt
     3301 gcaacaagtg gcagttccag ctattagcat tgctttatta ggaatgattg aaagcttgct
     3361 atgtggtgct tcggctggcc gaatggcaaa taagccctta gatagcaatc aagaattaat
     3421 ggcgcaagga attggaaatc tgttgcttcc tttctttggc gggattcctg ctactgcggc
     3481 catcgcccgt acaagcgtgg ccatcaaatc tggtgcacaa actagattag ctggtatgat
     3541 acatgcagtt gtcttactgc tctccatgct tgtttttgcg ccagtgatgt cacagattcc
     3601 aatgccagca cttgcaggtg ttttgattgt gacggcttgg cgtatgaatg aatgggaaac
     3661 gatcaaggag ctatttacta ataaatattg gtcagcagtt ctattgttta tccttacgat
     3721 gatttgtaca gttatctttg atttaagtat tgctatcgtg atcggggtga tcggcggctg
     3781 tgtatttttt gttgtcaaaa gcgctgctat cacgatttca ttagaaacga tcgattggca
     3841 aagaatgggg ctgccagaaa cggaaaagct gaataactgg acagtagtgt atattagtgg
     3901 tccactgttt tttatgtctt cagaaaaact caaaaataaa ttggaagaac taaatcatca
     3961 cgatggtgtc attttctcca tgcgaggcgt accaagtatt gatttgacgg cacgtgcagt
     4021 ttttgatgaa tttcaagaaa aagcagagca aagaggacaa acgattatct atacttcctt
     4081 gcagccagaa gtagaaaaac aactttctca cttgtgggaa catacattca cgcatcaaca
     4141 tcaaacagtt gctcatgcgt tgacagcttt acatgcgaaa tatactgcaa ttagccatta
     4201 aaacaatgaa aaagaacaga caagactgta aactgaagat tttatggtct tgtctgttct
     4261 ttttaatatt gctgtaaaag tgaagctagt acttgccagt cttgatggat aacaggtaca
     4321 agggagcgat tgtagaaaat agctgctgga tgaaaaagtg gaagaattcg atagtttttt
     4381 tctgaccatt cgtaggcatc cttttgttct gttaattgta agacaggccc tgtatagagt
     4441 tgaccatgat ttttggtaac cgtatattct ttgccaagta aacgttgaag accaatgttt
     4501 ccaagtgtcg caattaattt tggctggacc ttcatcagtt cgtaatccaa tattggtgca
     4561 tgcgcaaata cttcggaacg agtaggtgtc cgatttggat aaacagtttc tgactgttgt
     4621 gtccgtttat tgatccgatg agtcacgcga tacgggcggc tccttacagc actggtgata
     4681 tacacctctt ctcgtgttaa gccagttgaa gaaagtgcct gcatcaattc ttttccagct
     4741 tgaccgctaa aaggaacatg cgtatcaatc tctgttttcc ctggggcttc tcccacgagc
     4801 atcagtttag gatgaagagg tccttgccca gcgataaatc cttctacagg acggccgttc
     4861 attcttaaag aagcttcttg tatcagttct tttgggtatt ccatttatgc tcctcctttt
     4921 tcttttatta taagaataaa agaaacacaa tgcgactatt ttatttggta gaactgtgag
     4981 gatagaaaag cagaagtatt gcttgaattt ttgttttact ttttgatttt aaatttagtt
     5041 gactaacggt tgctgttctg gtaagctagt actgttgtaa ttgtggactc cacagctaca
     5101 accgctcaga acaagtaata agcacagcat atgctgtcac ttcggatggc gagtctaagt
     5161 atatcagaag gaggtgcttt atagcatgta cgcaattatt aaaactggtg gtaaacaagt
     5221 aaaagttgaa gtgggacaag caatctatgt tgaaaaactt gacgttgaag caggagaaaa
     5281 agttgttttt gacgaagtta tcttagtagg cggagaatct acaaaagtag gcgctccaac
     5341 tgttgcaggt gcaactgttg aaggtactgt tgaaaaacac ggcaaacaaa agaaagtcgt
     5401 tactttcaaa tacaaaccta aaaaacattc acaccgtaaa caaggtcatc gtcaaccata
     5461 tacaaaagtg gttatcgacg cgatcaacgc ttaatagcgt ttgagaaaga aggcctatat
     5521 atgatcaaag gaacatttaa gcgaaatgac gcaggtcaga tcgtttcatt cactttgacc
     5581 gggcatgctg atgctggtcc ttatggaagt gatatcgttt gtgcgggtgt ttctgcgtta
     5641 gcgatcagca ctgtcaacgg gattgcctca ttagccggtt ttgagccgat tgttgaaatg
     5701 aatgaagaag aaggcggtta tctttatacc gaagtcactt caggaatgac acaagaacaa
     5761 aacaatattg ctgagattct attagaaaat cttttattag gattacaatc gatagaagct
     5821 gaaaactcag aatatatcca aattaaaaca attaatgata aataggaggt gcagaccatg
     5881 ttgttatcta tgaatctaca attatttgcc cacaaaaaag gtggcggttc tacatctaac
     5941 ggccgtgact cagaatcaaa acgtctaggt gctaaacgtg ctgatggaca aacagttaca
     6001 ggtggatcaa ttctttaccg tcaacgcggt acaaaaatct atccaggtgt aaacgtgggt
     6061 atcggtggag acgacacttt atttgcaaaa gttgatggcg tagtacgttt cgaacgtaaa
     6121 ggccgtaaca aaaaacaagt gtctgtttat ccagtagctc aagaagctta attgaatctt
     6181 atgattaaag aggttgtgac aagacttttg tcacagcctt ttttgtttat aaaaataaaa
     6241 gttgtagtgg aggctacgaa gtttactttg tctttttaca cttaatattt ttttgccaat
     6301 ttggaatcag gatgttttga taactatcgt tttcaattga tggaagctat attgtttgct
     6361 atcagtgaaa taattatatg gtaaacagtg ttctttttct tgtttccatt ataaaaatcg
     6421 tatcgggatt tttcgttctt ttaaattgaa catttctatc tatccgcgtg ttatcaacaa
     6481 aacaaaatca ggtaaatagt tgacgaaaca gaaataaata catagtatta tgttttgaag
     6541 cctaagtaca atagtctata gaaaaggagc gataccattg gcaaaacgca ttgaccgaat
     6601 ttatatgtat gtcaaagaaa aaaccgctca tttaacaaag gcagaatatg atcaaggagt
     6661 aacaacacag gaaattgctg aagtgttagg gattcagcga acaaatagca gtaaagatct
     6721 gaatcagcta gtgcgcgaag gaaaactgat aaagacagac ggacgaccag tacgttacat
     6781 atatcaaaca ctcgttacgc acgtaaagcc agttacaaaa catgtagtca gttacaaaga
     6841 aacgccggtc tctgaagaaa aaaatacact ccaaatagat actaaggata tctttgcaaa
     6901 aatcattgga gctaacggca gcatgaaaaa ttcagtggaa caggcaaaag ctgcgatttt
     6961 gtatccgcca agaggattaa attgtttgat tacaggacct accggatcag gaaagactta
     7021 ttttgcacac gcgatgtatc attttgcaaa agcgcatcat gtaatagatg aggaaacgga
     7081 actgattgtt ttcaattgtg ctgattatgc caataaccct gagcttttga tgagtcattt
     7141 atttgggtac gcaaaaggtg cttttacagg cgcagatgaa gaaaaaatgg ggattatcga
     7201 tcaagcggat ggcggtatgc tgtttttgga tgagattcac cggttgccgc ctgaaggtca
     7261 agaaatgatc ttttacttta tggatcatgg aacttacagt cgtcttgggg aaacaacgaa
     7321 atcccatgaa gcaaatgtac ggatcgtagg tgcgaccaca gaagacccag gttcttctct
     7381 attagaaact tttgttcggc gtatcccaat caatatcaaa cttccggctt ttgaaaaacg
     7441 tcctgcaaat gagaaagtcg atttagtaaa aatcatgatt gcccacgaag caaaccgcac
     7501 gcagcgaaag atttctttga cagaagatgt agcaaaagca ttgatcggca gtgtgaccta
     7561 tggaaatata ggacaattga agtcaaatat ccagttagta tgtgcacgag gatttctaaa
     7621 tcatatgcaa tcacccgaaa tctcgatcac aatcgatgat ttgacagaag ggattcgtag
     7681 cggattgatt caattagcaa gcaaccgcag tgcaatggca gagctttcta aaatattgga
     7741 gccaaagata actgtagcac caaatgatac gatcatgaaa atccaatcgg actcctatga
     7801 gttaccttat aatctatatg atatcatcgg agataaagca gctttactga agtcggatgg
     7861 tttggatcaa gaagcaatca atcatttcat ttctacagat atcaacatcc atctgaagtc
     7921 tttctataaa gaccatggtt tttcttttaa tgcagacagc aaattagcag aatttgtaga
     7981 tccgaaaatc atagaagtga ccaatcagat ttatgtaatg gtcaaaagat ctttaccata
     8041 tgagtttcag caaaacttta tttatgctat gagcttgcat atcagttctt ttttgaaaaa
     8101 aatcaactta ggagaagaac ggcatacgaa tgataacatc cgtgagatgg cttttgattt
     8161 tccagatgaa tatgctgttg cgaaagaagt gcgtagttat atcgaaacgt attttcaagt
     8221 ggagattcca gaaagtgaag actattactt gaccgttcta ctcgtctcgt tacgggcaaa
     8281 tcaagcaagc ggcagaatcg gtgtagtcgt tgcagctcac ggaaacagca cagcaagcag
     8341 tatggtccaa gttgtccaac agttgttaga tgcagagaac gtacgagcag tagacatgcc
     8401 tttggatatg gatccgaaaa cagcattgga ccgaattgaa cgcaatgtac aagaagtaga
     8461 tgaaggaagc ggggtcatac tcttagtaga tatggggtcc cttgcgtcgt ttaattcgca
     8521 aattcaaaga gacactggaa ttccagtacg tactgttgat atggtaacaa cttctctagt
     8581 tttggaaact gtacgaaagg tctcagtatt agggacagat ctagatatgt tgcatgaatc
     8641 attgaaaaat tttcgaggtt acgcagaaat atcttctgta gaatctgttc aggaagcaaa
     8701 acctattcat aagaaaaaag ctgttttggc tatctgcgca tctggcgaag gtacagcaca
     8761 aagaatcaaa gaattgatcg aacgagcggt aaacaagcgt caagaaacag aattagaagt
     8821 tctagctttg tcgattgtgg aagtgaaaga agaactgcca gttatccaaa aagaatatca
     8881 gatcatagct actactggga ttactgattt gaatatccaa gccccattta ttcctttaga
     8941 acgttttatt gatcaaaata tcgaagtcat tttggatcag ttgctgatgg aatcagaatt
     9001 ggaggaaact gaattcatct ccctagatga agagtcagct aaaaatacgt gtgttgagtt
     9061 tatctctgat aacttcatct ttattaatgg tagtaagtta atcgatccaa tgtggcaatt
     9121 ttctacacaa atctctcaga cgacgggaat cggcgatgaa gaatatggat tcaaaatcaa
     9181 cctagtgatg cacacagcag gaatgatcga acgtattatt cggaatgaac cattgactgt
     9241 ggaagaaaac gaattgacaa atacaacgaa tgatcctctt tatagccaat tggcagcctc
     9301 agttgttctg ttggaagatc agataaaagt aaaagtgccg atagaagaaa tgtattatct
     9361 gctacgtcta gtccataatc agttagataa aaaagaatac actgtgccat gaatacacaa
     9421 aaacgataca cttttatagt gtatcgtttt tgtgtattgg ttaagaatcc tttaaattca
     9481 attagtgtat cgtaaaaaag ttggcacgct ttttgcttat agtagtgtgt gctaggaggt
     9541 ggttgttcac acagaaatga taggggtttc tgtgaagtag tgaggaagaa ggagttttgc
     9601 agatggatat tcgattggca cgtatagatg atcgtttaat ccacgggcaa gtcgcaacag
     9661 tttggtcgaa gcaaacaggt gttgaacgta tcattgtgat cagcgatgta gtcgctcaag
     9721 ataaattgag aaagtttcta ctaaaagaag ctgcaccgcc ggggatcaca gcaaatgtaa
     9781 taaccgtgca gaaaatgatt caaatctatc atagtggatt attatcacga gcaaaagtga
     9841 tgcttttatt caccaatccc caagatgttg aagcggttgt ccgctgcggt gtacagttga
     9901 agtcgttgaa tatcggcggt atgagtttta ctgaaggcaa atcaatgatt accaattttg
     9961 tttcagtaaa tcaacaagat attgattcct ttcgttactt gaacagtcaa gaagtggagt
    10021 tagagattcg aaaagtacct gctgacagga aaatcctttt gatggaacta ctagcgaaaa
    10081 tcaaaaaggt gtagcaagaa tggttgttgg aaaaggtcaa ataattctgt tgtaatggtt
    10141 agcaattatt aatatttttg aatttgttac aacaaaagta aataggaggt ataaatatgg
    10201 tagggattat cctagcaagt catggacaat ttgctgaagg aatcttacaa tccggttcaa
    10261 tgatctttgg cgaacaagaa aacgtcaaag ccgtgatttt gaaaccaagt gaaggtccag
    10321 atgacttgag agccaaattg gaagaagcgg ttgcttcttt tgataatcaa gacgaagtgt
    10381 tattcttagt tgatttatgg ggcggaacac cgttcaatca atcaaacaca ttattcgaag
    10441 agcacaaaga taaatgggca atcgtcagcg gtttgaattt accaatgttg attgaagcct
    10501 acgcatcacg cttttcaatg gaatctgctc atgaaattgc agcacacatc attgaaacag
    10561 caaaagacgg tgtaaaagta aaaccggaag aattagaacc ggcagaagca ccgaaagcag
    10621 cagttgaaga cgcacaacct aaaggagcac ttccagaagg aactgtggtc ggagacggaa
    10681 aaatcaaata tgtattagca cgtgttgact cacgtctgtt acatggacaa gttgcgacag
    10741 cttggacaaa agcagtgcaa ccaaaccgta ttatcgttgt ttcagatgca gtatcaaagg
    10801 atgatctgcg taagagacta atcgaacaag ctgctcctcc aggtgtaaaa gcaaatgtta
    10861 ttcctattag caaaatgatc gaagtagcaa aagaccctcg ttttggaaat accaaagcat
    10921 tgcttttatt tgaaaatcct gaagacgtat tgacagctgt tgaaggtggc gtagacatca
    10981 aagaattaaa tgttgggtca atggcgcatt cggtcggtaa agtcgttgta agtaaagtat
    11041 tgtctatggg acaagaagac gttgaagcgt ttgagaagct tgaacaaaaa ggtgtgaaat
    11101 tcgatgtacg taaagtacct aatgattcac gcgataacat ggatgatatt ttgaaaaaag
    11161 caaaagctga attagcaaaa gcgtaataaa taatattaaa atttatagga ggcttatcat
    11221 gtctattatt tcaataattt tagtcgtatt agttgccttt ctagctggta tggaaggtat
    11281 tcttgatgaa tttcaatttc atcaaccact agtggcatgt acattgatcg gtttagttac
    11341 cggtaacctc gaagctggta tcgtacttgg cggtacgctt cagatgatcg ctttaggttg
    11401 ggcaaatatc ggtgccgctg tagcaccaga tgctgcatta gcatcagtag catcagcaat
    11461 tattttagtt ttaggtggac aaggtgttaa aggtgttcct tctgcgatcg cgattgccgt
    11521 tcctttggct gttgcagggt tgttcttaac aatgatcgta cgtacaatcg ctgttccaat
    11581 cgttcatttg atggatgcgg ctgctgaaga aggaaacatc cgtaaggttg aaatgtggca
    11641 catcattgct gtatgtttac aaggggttcg tatcgcggtc ccagcagcag cgttgctatt
    11701 tatcccagca caaactgtgc aatcattcct tgaatcaatg ccagcatggt taacagatgg
    11761 tatggctatc ggtgggggaa tggtcgtagc tgtaggttac gctttggtaa tcaacatgat
    11821 ggctacaaaa gaagtatggc cattcttcgt tattggtttc gttgtagctg caatttctca
    11881 attaacattg attgctctag gtgctttagg tgttgcatta gctcttattt acttgaacct
    11941 ttctaaaatg ggtggttcat caaataatgg aggcggaagc aactctggtg accctttagg
    12001 cgacattttg aatgactatt aatcttgaag gaggaaaata aaatggcaga agaaaaaatc
    12061 aaattatcaa aaaaagatcg tttagctgtt gcgtggcgct ctaccttcat tcaaggttca
    12121 tggaactacg aacgtatgca aaacggtggc tgggcatttt ctatgattcc agcaatcaaa
    12181 aaattataca aaacaaaaga agaacgttct gcagcattaa aacgtcactt ggaattcttt
    12241 aatacacacc catacattgc ttctccaatt ttaggggtta cattggctct ggaagaagaa
    12301 cgtgcgaatg gcgcgcctgt tgatgatgta gcgattcaag gggttaaagt aggtatgatg
    12361 ggtcctttag ctggtgttgg tgacccagtg ttctggttta cagtacgtcc aatgctaggt
    12421 gcactaggtg cttcattagc tatgggagga aatatccttg gaccaatcat cttcttctta
    12481 ggttggaaca ttattcgttg ggcattcatg tggtatacac aagaatttgg ctacaaagct
    12541 ggttctaaaa ttactgatga cctttctggc ggattgttac aagacgtaac aaaaggtgct
    12601 tcaattttag ggatgttcgt attagctgcc cttgtacaaa gatgggtatc aatcaaattc
    12661 ttaccagttg tatcaactgt aaaattagat aaaggtgctt acatcgaatg ggataaattg
    12721 ccttcaggtg gtgaaggaat caagagtgca tttgaacaag ttaataatgg cttagccctt
    12781 tctcctgaaa aggtaacaac attacaagac aacttagacc aattgattcc tggtttagct
    12841 gcattaggat taacattgtt atgtatgtgg ttgcttaaga aaaaggtttc tccaatcgtt
    12901 atcatccttg gtttattcgt agtcggtgtt gttggacacg tcatcggact tttataatcc
    12961 ataaaaatat agctcgggcg ttgtcccgag ctttttttat atagtatact gttcataaac
    13021 aaatatctgg agggagaaaa tggttcaatc aatcaataca aaagtagact tggtaattga
    13081 tgctacttcg tttacgggtc tgacagatta cgggcaaatc atgataggtg ataatggttt
    13141 tgagttttac catgcgcgag atagacgcaa atatatccaa attccatggg aagaagtgga
    13201 ttatgtacta gcatcagtca tgctaaaggg aaaatggatt cctcgatatg ccattcaaac
    13261 gaaaaaaaat ggtacataca cattttcttc caaagaacct aaaaaagtgc ttcgtgcgat
    13321 cagagaatat gtagatgaag atagaatggt acaatcattg agtttctttg atgtagtgaa
    13381 acgtggtatt cgtagtatgt ttaaacgcaa gtaaaaaata aagaaaactt ctaagaggag
    13441 gttttcttta tttttttact ttgcagaaga aaatatgaaa tagcaaaacc aattatttgt
    13501 cccctttaaa tcccatatgc taaaattgat tagcaaagaa ggaggttata ttatgagttg
    13561 gttatggaca ttgattgttg gagccgtgat cggagcaatt gcaggtgcga tcaccagtaa
    13621 aggaaattcg atgggctgtg taacgaatat cattgctgga ctagtcggtt caagtattgg
    13681 acaagcgatt ttcggcgaat ggggaccaaa agctgcaggt atggcactta ttccatctat
    13741 tttaggcgca gtgattttgg tagcagttgt ctcatttttc tttggaaaac gatcataagt
    13801 ttttacaaaa tagtaaacta aaaggagttg cagcgagact gtgactcctt tttggcttta
    13861 tttatttaaa ataaaaagaa gttttccgga aatttttatt taactgtcgt gtacgaagaa
    13921 aagtttgcta taatggaaaa aagcgtgcaa agaaagaggg agtttcatta tgatgctacg
    13981 tgtagaaaaa ttgcgcaaaa aaatgcaaga agaaaatttg gattcatttt tggttaccag
    14041 tccgtacaat cttcgttact taacgaactt tacaggtacg acaggattag ctgttattac
    14101 tttggaaaaa gcatttttta ttactgattt tcgttatacc gagcaggcgg cagcgcaggc
    14161 tcaagggttt gaaattatta aaaatgtcgg acctattttt gatgaggtag caaacttagt
    14221 acgcaaagaa gaactgcact cattagcttt tgaggaaaca acagtctcat ttttagaata
    14281 ttcggtttta gaagaaatca tcgatgcgga attagtacct gtttcacaga tgatcgaaga
    14341 actcagagaa gtgaaagatg aagaagaaat cgctattatc gaaaaagcat gtcatattgc
    14401 ggatatggca tatgatcaca tcctgaaaat gatccaaccg ggcatgactg agattgaagt
    14461 cgcaaatcaa ctagactttt atatgcgttc tcttggcgct tcaggtgttt cttttgatac
    14521 gatcgttgct agcggattgc gttcggctat gccgcatggt gtagcaagca aaaaaatcat
    14581 cgaacaaggt gacttgatca cattggactt tggttgctat tacgaagggt acgtttccga
    14641 tatgacacgt acattcgcta ttggtgatcc tggggaaaaa ttaaaagaaa tttatcaaat
    14701 agtcttagat gctcaattag cagttattga tgctgcaaaa ccaggaatga ctggtgtcca
    14761 attagatgca gttgctagag attatatttc aaaacatggt tacggggaag catttggaca
    14821 ttctacagga catgggatcg gtttagagat ccacgaagga ccaaacgttt cttctcgtgc
    14881 agaaaagcaa tttgtaccag gaaatgtgat cactgatgaa ccagggatct acttaccagg
    14941 aatcggtgga gtacgtattg aggatgactt gttgatcaca gaagatggaa atcgtgtgtt
    15001 gactcgttca ccaaaagaat tgatcatctt atagtttcat caatagaaga agtaagcgta
    15061 aaaatcagtg ttcacagact ttgttataga aaatcactcg atttaatgat acactataga
    15121 agaactttta agctgtaaga gataataaag ttgggtgttt tctccaattc atagcagttt
    15181 gcagctgatc ggcggcagtt ttcttaccac gtgatcacta tgccgcatca tagagaattc
    15241 ggatgtatct cgtgcttagg caaagggagg aataaataaa tggcagatga aaaaaatttg
    15301 attttaaacg caaaccaaga tttaggagag atcgtgatcg ctcctgaagt catcgaagta
    15361 attatcggga ttgcagcttc taaagtagaa ggcgtatacg gaatgcgcgg aacttttgcc
    15421 agcaatgtca ctgaattatt aggacgtgca gctcacggaa aaggtgttta cttagcaaac
    15481 gaggaagatg gcttgaaagt agatctttac tgttatctag aatatggtgt ttcagttcct
    15541 aaagtagcaa tggaaatgca ggaacgggtc agccagcaag tccttttcat gacggatatc
    15601 gatttatctg aagtcaatat ccatgtagta gctgttgttc ctgaaaaatt agaacaacca
    15661 acaatcgaag aactttttga agacgaagag gaagagaatg aataaagaat tatcaagaca
    15721 cgagattcgg gaaatggcat tgcaggcttt atttccatta gacttcaatg cagacctaac
    15781 aaaagaagat gcgattttca acgcaatcga attagaccac cgcgacatga tcaacgaaga
    15841 tgaatcagag tttgttcctg tttatcttga tacgctagtt ggcggtgtct gtgcaaaaaa
    15901 agatgaattg gataaagtga ttgaaaaaca tttaaaaaac aattggcaca tcaatcggat
    15961 ctcaaaaatg gatttggtga tcttgcgtat cgcgattttt gaaatgactt atgtgacaga
    16021 tgtaccagcg gctgttgctt taaatgaagc aatcgagttg gcaaaaacat ttagtgatga
    16081 ccgttcacgt aaattcgtca acggtgtttt atccaatgta ttgaaagaat tagaagctgg
    16141 ggcgtaagtc ctagcttttt tctatgaatc atcataaacc tgttataaat cgggaaaatt
    16201 ctatggtaga atgaaaacac aaaaaatatc tgggagtggt tagatggcag aattgattaa
    16261 cggaaaagaa ttagctgaaa aaatgcaggc agagattgct gaaaaaatcc aaaaattgaa
    16321 agataatggt atccatcccg gattagtcgt tttattggta ggggaaaatc cagccagcca
    16381 aatctatgta agaaataaag aacgtgtagc aaaagaaatc ggaatccatt cattggtaga
    16441 gcgttatcct gagacgattt ctgaagacga actacttgca gaaattgaaa aatacaatca
    16501 agatcctagt tttcatggta tattggtcca attaccattg ccaaagcata tagatgaaga
    16561 aaagatcctg ttagctatcc gccctgaaaa agacgtagac ggttttcatc cgatgaattt
    16621 aggcagactc cttgcgggta aaccggacaa gattccttgt actccatacg gcatcatgaa
    16681 aatgtttgaa gcttattcta tcgatccggc gggtaaacga gcagtagtta tcggacgaag
    16741 caatatcgtc ggtaaaccga tggctcagct actcttgatg gcagatgcaa ctgttactgt
    16801 tgctcattct aaaaccgaaa atctggctga actggcacaa gaagcagata ttttagtcgt
    16861 agcaatagga cgtggacatt ttgtcacaaa agaattcgta aaaccaggtg ctgtagtgat
    16921 agatgtaggg atgaatcgtg atgctaacgg taagctgatc ggtgatgtga aatttgatga
    16981 agtggagcct attgcaggct ggatcactcc tgtgccaaag ggtgtagggc caatgactat
    17041 cacgatgctg atgtatcaaa ccgtaaaatc tgccgaagct tttggagcag gtgaatagat
    17101 ggcacaagaa tatttaactg tcactacatt aacgaagtat ttgaaaagaa aatttgatgc
    17161 cgatccttat ttagaacgcg tatatttaac aggcgaaata tcgaatttcc gtttacgtgc
    17221 caatgcccat caatatttca gcttaaaaga tgaccatgca aaaatatctg ctattatgtt
    17281 caaaggagca ttccaaaaat tacgattcca gccaaaagaa ggaatgaaag tccttgtcgt
    17341 tgggcgtatt tcattatatg aagcgggagg ttcttatcaa atctatgtag agcacatgga
    17401 gcctgatgga ataggtgctt tatatcaaga attggaagaa cgaaaagaaa agctgatgaa
    17461 ggaaggcctt tttcaagggc ctaaaaaaat gctgccacgt ttcccaaagc gaatcgcggt
    17521 ccttaccagt ccaagcggag cggttatccg cgatatcatc acgacggtga aaagaagata
    17581 tccaattgtc caattggttt tgttcccaac agttgtacaa ggaaaccaag cagcggacaa
    17641 tgttgtcagg aatatccaga gagtagaaga gatgggcaat ttcgatacaa tgatcatcgg
    17701 tcgtgggggt ggttcgattg aggatttatg gccgtttaat gaagagcggg ttgctcgggc
    17761 gatttatcaa gctaatacgc cggtgatttc atctgtagga catgaaacag ataccacgat
    17821 cgctgattta gtagcagatg ttcgggctgc cactccaaca gcagctgcag aattagctgt
    17881 tcctgttttg tcagaagagt tgatgcgcat tcaagaacgg caagctagat tgcagcaggc
    17941 gtttgtacgc cagatccagc gcaaacaaga acggttcgag cgtgctcgac gttcctatgt
    18001 atttcggcag cctgaaagat tgtatgaagg tcagaccatc aaattagacc agttgaaaca
    18061 gcgattatat caagctacac agcaaattta tcatgcgaaa gaaaaacaaa cgatcgcatt
    18121 aagccaccaa ttggaacaag tggctccgct ttatcgagtt aaagcagctc gccaagaaac
    18181 tgcttatcta gaaaaacgct tagaagagaa aatccaacaa tatatgaaac aacagcagca
    18241 gcgttttgct caagctgttc aatctcttga tttgttgagt ccattgaaaa tcatgggaag
    18301 aggctacagc tatacgacgt taaatgatca agtagtaaaa agtgtcaccg agattcatca
    18361 aggggatttg ctgacgatcc attatcaaga tggaatcgtt aaaggacaag tacaagagat
    18421 agacaaagga gaataaaaat atggaaaatc caacttttga agagtcactg caagaattag
    18481 aaaaaattgt catgcagtta gaacaaggag acgttccatt agaatcagct ctagattcgt
    18541 ttaaacgagg aatggaatta agcaaacatt gccaagatac attagctaaa gcagaagaga
    18601 ccttgacgaa aatgatgaca gaatcaaatg aagaagttga ttttgacgga aacgaggaaa
    18661 gctgagatga gattaactga cttttcagct attcatctac ctgctgtaga aaaagaaatc
    18721 ttgtcttttt tagatgagca tacaacggat agaggactct atgatgcgat gagttattca
    18781 gtgaaagcag gcgggaaaag aatacgtccc ttattacttt taacggccgt tgcttccttt
    18841 gatgagccta ttgatgttcc cgtctatcaa gtagcagctg ctttagaaat ggtgcatacg
    18901 tattccttga tccacgatga tttgcctgcg atggacaatg atgatcttcg tagaggaaaa
    18961 cctaccaatc ataaagtatt cggcgaagca ttagctatat tggccggaga tggtttgctt
    19021 acaggagcat tccagctaat cagtatggca cacttaggta attctcctaa gcttctgctg
    19081 cttcagcaat tagctgtttg tgcgggatca caaggtatgg ttgctgggca agctgcagat
    19141 atcgagggcg aatcgaaaaa gctttcttta gaagaattag catttattca tgaaagaaag
    19201 acagggcatt tgattcgtta tgccttgctg gctggtggca tattagcgaa gcaacctgaa
    19261 gaaatcttat tgctacttca acgattagcc gaacatttag gtttagcttt tcagataaga
    19321 gatgatctac tcgatgtgat tggtacaacg aaaacattag ggaaaacagc tggtaaagat
    19381 gaacgtatgg aaaaaaatac ttacccgcgt ttattaggcc tagaaaagac acgtgaggcg
    19441 ttggagattg agctgttatc cgccaacaag atcatagaca aattagaaga ggacgttttt
    19501 tcttttgatg gcgggctgat gcgccagatg atcaagcaat ttgatgtgga gaaatgaggg
    19561 aaagaatgga aaaagaacgc gtagatgtat tagcggtcaa acaaggatta tttgaaacaa
    19621 gagaacaagc caaacgttca gtgatggctg gtttgattta caacgaaaaa aacgagcggt
    19681 ttgataaacc gggagaaaaa attccagtga gtagtgaatt aagagtcaaa ggtaaaaaac
    19741 ttccttatgt ttctcgaggg ggattaaaat tagaaaaagc actcaaagca tttgatctat
    19801 ctgttgaagg aaaaacattg ttagatattg gtgcatccac tggcggattt actgatgcag
    19861 ctttgcaaaa tggcgcaaaa atgagttatg ctttagacgt tggctacaat cagctagctt
    19921 ggaaaataag acaagatcct agagtcgtcg taatggaaag agtgaatttt cgttatgcaa
    19981 aacccgaaga ctttacctta ggaattccag aagttgctgt tatcgatgtt tcttttattt
    20041 cactaaaact gatgctgcct ccattacatg ctattttaaa agaagaggga gaagtgattg
    20101 ctttaatcaa accccaattc gaagcaggca gagaagctgt agggaaaaac ggaattgttc
    20161 gtgaccctca gacccatcaa aaagttttag aagacatttt gtcatttgcg gcacaaggtg
    20221 gatatgatgt gctggaatta agttattctc cgatcacggg gggagaagga aatatcgagt
    20281 tcttggctca cttgaggaaa gtacccgaaa gcggaacaat caattcagct attaatatgg
    20341 cagaagtggt ttcaaatgca cacgaacagt ttgaccataa ataaattgta actgtgaaaa
    20401 tcaagtgaga agtatgcgga ttcttcttta ttataccaag gaaataagct atcattgatt
    20461 aagaagaata tgctaaggag cgaagacgta atgagaaaaa aagatcggca tcgtttgatt
    20521 actcgattgt tgagtgagca agatatccga aagcaggaag aatttgttga aatcctgaaa
    20581 agcaaaggaa tatctgttac tcaagctacg atttccagag atataaagga actgaaactg
    20641 atcaaggtac cggcttcaga tggcggctac cgttatagca taccagctga aacaaacgaa
    20701 gatgtaaata ctaaattgga aaaactatta aaagacgctt tcgtggcagt tgaccaaatg
    20761 gaaaaatttg ttattttgaa aacattgccg ggaaatgctt cggcagcagc aaatttgatt
    20821 gataaacgtt acaagaaaga attattttcg attatcaatg atgacgataa tgttttgatg
    20881 attacacgaa ctgaagacca ggcagtttta ttgaagaaag acttcctgca ttacctttaa
    20941 gttgaggagc gaatgtgatg ctacaagaaa tcagtattac taactttgcg attattccgg
    21001 aattgcggct ttcttttcac gaaggaatga ccgcgctgac cggagagact ggtgcgggta
    21061 aatccattat tatcgatgct ttaggtctgt tagcaggagg aagaggttca agcgattata
    21121 tacgtcaagg agcagaaaaa tgtgtgcttg aagggttgtt tgaactacca aaacaagaag
    21181 gattctctga actgatggtt gaattaggga tcgaaacaga tgaggacaat ctgattgttc
    21241 gacgggatat gtccttaact ggaaaaaatg tttgtcgagt gaatggtcat attatcacat
    21301 tagctaatct gagaagaatc ggcagttatt tagtagatat ccaaggacaa aacgaacatc
    21361 aggaactgct ccagcctgaa tcacatcttg ctttattgga tcgttttgga gatgtagctt
    21421 tccaacagaa gaaaaagagt tatcagcaag aatatatctc ttatcgtgag ctggaaaaaa
    21481 gggtacgaaa agtgcagcaa aatgaaaaat cctatgttca gagaatagat atgctccatt
    21541 tccagcaaga agaaatagct gctgctgatt tgcaagtcgg agaagaagag aaattaaaag
    21601 aagaaagaga aaaacttagc aattatcaaa aaattgctga tggattagct gcaggttacg
    21661 gagcactgac gaataatgaa caaaacagtg tagatggtgt tggcttggcc gtttcagaac
    21721 tgcaagggat cgcccatcta gatgtggagt atgaagcaat ttatgaaaat atccaaagtg
    21781 cttactattt gctgcaagat gcaatcggcg atatgagtcg tcagattgat ttattggagt
    21841 tagatgagag tcgtttagaa gaagttacgc aacgtttaga attaatccga cagttgaaac
    21901 gtaaatacgg cgaatccatt gagtcaatat tggcttacta tgacgaaatc accgaagagc
    21961 tagcttcttc tgatttttca gaaggtcaat tggacaaaat gaaagaggaa ctagagcaaa
    22021 aagagcttct acttcagcaa caagccgcag atcttcatga agcgcgtaaa aaaatcgcaa
    22081 aagaattgga aaaatccatt ctgcatgaat tgaaaagtct gtatatggaa aacactgagt
    22141 ttgaggtacg tttcttaaaa gaagaaaata gacaattgaa tcatgatgga tttgaccaaa
    22201 tcgaatttta cattacgacc aatccaggag agccactgaa gcctttggtg aaagtcgctt
    22261 ctggtggcga actgtctcgg atgttgcttg ctttgaaaac tattttttct tctgagcaag
    22321 gtgtgacaag tattattttt gatgaagtgg atacaggtgt gagcggaaga gttgcacagg
    22381 ccatcgcaga taaaatttct caaatatcga aatactctca agttttgtgc atcactcatc
    22441 tgcctcaagt tgctgcagtc gccgactatc aatattatat tgtgaaagca gtgattgatg
    22501 gacgtacgca aacttctgtt tcagagttga aaacaaaaga aagagaagaa gaaattgcac
    22561 gtatgctggc tggaagtgag attacaaaac tcacactcga acatgcaaaa gaactattga
    22621 aactcgcaaa aaggacctga gccacctcgc agtggttcag gtccttttca gcagctgata
    22681 gctatatttt atttttacat gacactcatt acagcagtca atacaacaga tccaatcgtt
    22741 aaaatcagca ttgtccaaat gacaaatttt gttactttag caaaagtact tgtttgtttt
    22801 ttttcacgca ttgttttcaa cctcgtcatt aattagttga ttcaagtgta caatcttttt
    22861 tccatttcca caagagggct tttttataaa aacttccctt tttttaaata ataggtagtt
    22921 acgatacaaa taaataatgt taatagaact agccatataa aggcatcatc cctttcagca
    22981 ataggtaact taatgttcat tccatagata ctgccgacga ttgtcggtat agttaaaata
    23041 atcgttaaag aagttaaaat tttcataata ttattcagat tattcgatac gattgctgaa
    23101 aaagtctgat tcatttgttc tactagtttt aattgaatgc gagaagtagt cattgcctgc
    23161 tctgtttcca ccaatacatc acgtaaatga tttttcaaag catgattttc ttggaaaagt
    23221 ttattttgcg ctagttcttt caatgcatct aaattagcag aagtcgcttc tctgaaataa
    23281 acgaggcttt tttggatatc catgatttga taaagttgtt tgttttcagt ggatacttga
    23341 agtttggctt ccaaagtatc gcattgagat tgaagcgtag ctaaataact gttgtaagag
    23401 agtgtgacct gccaaatgaa ttgaaagaag atattgagat taggcgaaat atcacttttt
    23461 ggaaaggtct ggctaaagac tttctttaaa aacagtggtt catgattcgt aattgtgata
    23521 agcttctttt tgggcgtcaa aataatagag agcgggtatg tatccacttg aaaataaccg
    23581 ctaggacttg ttttagcaaa aggatattgt aacaataata acgctggctt tgtcaacttt
    23641 gattgattga attcttctgt acgggaattt tctgcatcgt ccaatatgga agtgatgtaa
    23701 tctgacggaa tttcaaattg atgtgtcagc tgatgaatct cttcttgatt aggacgttct
    23761 acacaatacc agatcgtttg gtcagtttcc tttccggtat tacgcagttt ctcattatct
    23821 aatgtaaaat atttgatcat gattttcctc ctgttgcctg tttcttttat tataacggat
    23881 agggaattgg aatacatgga atgtgtttgt aagatataga atataactgc ataaatttaa
    23941 ataaaatgaa acagttgtgt aaagtatata cagtatactt gaagcaacga taaaaaagga
    24001 gtttacgttc atgggcacat tcacaccact tatcttgttt tggtatattt acccggtaat
    24061 cgttttgttt ggttgccaat ttcttgtaaa attattttcg ttaaatcgtc gttttaaaat
    24121 aaaagcccct gatttggcga ttcctttttt gtggggcggt atgcatgcat tgtctagaaa
    24181 tacagggaca atttctatct taccgttttt ctttatttct gttttgctaa tgggaatttg
    24241 tattgctgtt ttccaagcat attattatga agaaattatt tatccgcgtt atttcaagat
    24301 gacttggcgt ttggtttttc tgttgacaat cgtcttgtac gctgtcttga tcattgtgaa
    24361 catactttct tacctatgaa aaaaatagaa aagctgtaaa aagtctttta aaaggctttt
    24421 tgcagcttga tttttaaaaa aatgtttatg aataaaaaaa gttgttcccg gatttcccag
    24481 aaaaggtggt agaaagtggg ggattgtggt agactaattt tagttagtgg aggaatgggg
    24541 ggctttcaat gttgatgggt gaattccaac ataatattga tgcaaaagga cgtttaatcg
    24601 tgccctcaaa acttcgtgaa gaacttggcg aaaagtttgt tctgacacgc ggattggatg
    24661 gctgtctttt cggctatccg atgagcgaat gggaaaatct ggaagctaaa ctgaacgaaa
    24721 tgccgttggc taaaaaagac gcacgtactt ttgtgcgatt cttctattcg gctgccactg
    24781 aatgcgaact tgataaacaa ggacgaatca atatacccag cacgttaaga aattatgcag
    24841 ctttgacaaa aggttgcgta gtcatcggtg tatctaaccg aattgaaatt tgggatgaga
    24901 cacgatggca agatttttct gcagcagcag aagagaactt tgacgaaatt gctgaaagta
    24961 tgattgattt cggactataa gaataggaag aggctgacta atgactgaaa catttcaaca
    25021 ctatacagtc atgttgaaag aaacggtaga tggattgaac atcaaaccgg atggcgttta
    25081 tgtagactgt acactcggcg gtgccggaca tagtgaatat ttattaagcc aactaagttc
    25141 taaagggcat ctgtatgcgt ttgaccaaga tcaaaaagca atcgatcacg ccaagctgcg
    25201 tttagcttct tatgtcgaac aaggtatggt cacatttatc aaagcgaact tcagagaatt
    25261 agaagaacgc ttgagtgagc atgtagataa ggtagacggc attttgtatg atctaggggt
    25321 ttcctctcct cagctagatg aagcagaacg tggatttagt tatcatcaag atgcaccgtt
    25381 ggatatgcgc atggatcagt cagctccttt atccgcttat cacgtaatta atgaatattc
    25441 ctaccatgaa ttagtaaaga ttttcttccg ctatggcgag gagaaatttt ccaaacaaat
    25501 tgctcgtgaa atcgaacgag tacgaaaaac aaaaccaatc caaacaactg gtgagcttgt
    25561 cgaaatcatt aaacaagtca tcccagctcc cgcaagaaga aaaggcgggc atcctgcaaa
    25621 aagaattttt caagctgtgc ggattgcagt aaatgacgaa ctcggtgccg aagaggcctc
    25681 tttagaacag gcaatcgggt tattgaaagt caatggaaga atcagcgtaa tcacattcca
    25741 ttcattggaa gacagaattg tcaaaagtat gtttaaagaa tatagtacgg tacaagatct
    25801 accaccggga cttcctgtca tacctgacga attccaacca gaactgaaag tcattactcg
    25861 taaaccaatc cttccttcag aggaagagct aacagaaaac aatcgatcac gcagtgcaaa
    25921 gttacgtatt gcagaaaaaa taagaatgaa agaggagtag aatgatggca gaattaaaga
    25981 aaatgcaaga ttacgaatat gatttacctg aattggtgga tcagccggaa caacaaatgc
    26041 ctgattcttc ttcgaatcaa gaaatcattc ttccacaatc accggcaaaa cgattaaagc
    26101 agatttctgc tttggaaaaa attgctgtgg caagcgtggc gattgctatt gttgctttat
    26161 gtgttttaac agttatgctg cgcacaaata tcagcggagc tgaaaaagaa atcacgtcaa
    26221 tacaaacaga aatcaatgat aaaaagcaag aaaaaacgag tttagaacaa gaaaaaaacg
    26281 aactttctcg tactgaacgg attaaacaaa tcgcagaaga aaaaggattg tctattaatg
    26341 acgacaattt aaggaaagtg aagtaaatga gtcttaaaaa taaaattcgg cggtttatgg
    26401 aaaagaaaaa tctaaatccg atgaacaatc gcaaaaaagt aggtatcatt ttatttgcta
    26461 cgagtattgg attattcttt ttatttgccg tccgtttctc gtatatcgtt attggaggtc
    26521 atgttgctgg tacctcatta gctgaaaaaa caaaacagtt gtaccaagga agtgaagtag
    26581 taaaagcaaa aagaggaacg atttatgatc gtaacggtgt ggcgctcgca gaagatgcct
    26641 cctcttattc aattaaagcg atactctcaa aaacatatac ctctggggat aaaaaactgt
    26701 atgttgaaga aaagaatttt gataaaatag cagaaatcct gcataagaat ctttctatag
    26761 acaaaaaaga tgcactgaat attttagagg acggcgcaaa aaaagagctg tatcaagttg
    26821 aatttggtag ctatgggaaa aatatctccc aagaaaccaa gcaaaacatc gaagcggaca
    26881 tgaaaaaaga aggtgtcgct ggtctttatt ttgtcgacca tcaggctcgg atgtatccaa
    26941 atggcgtctt ttcttctcat tttattggat acgcagttcc agacaaagat gagaatggct
    27001 tagttggtaa actaggtttg gagtctgctt acaatgatat cttgagcggt aaagatggta
    27061 agattattta tcaaaaagat aattttcaaa atcctctgcc aggtacagtg gcagaagaag
    27121 aaaaagctgt cgacggacaa gatatctaca cgactttaga cagcagacta caaagttatt
    27181 tagaaacact gatggatcaa gtaaatgaag aatatcagcc agaagaattg acagcagtcc
    27241 tgatgaaagc aaaaaccgga gaaatcttgg caatgggaca acgacctact ttcaatcctg
    27301 aaacaatgga aggattgact ggaaaagatg ctatctggcg taatttcttg gtacaagata
    27361 gttatgaacc aggatcaacc atgaaagttt tcactactgc agctgctatc gaagaaggag
    27421 aattcaacga gaatgaaacc ttccaatctg gaaaaatcca agtagaagat gctacgatca
    27481 atgaccatga ctttggtgaa aaaggtgtgc tgaccatgcg tcaagctctt tcatggtcaa
    27541 gtaacgtcgg gatggtcata ctggaacagc gattaggcgg acgatggtat aactatttac
    27601 aaaaactggg atttggacaa agtactcatt ctggattaga tgatgaagta aacggtgctt
    27661 tacctacatt aaatattgtc gaccgagcaa tgagtgctta tggacaagcg gtaggtgtga
    27721 cgaatttcca aatgatgaaa gctttcactt ctattgcgaa taacggtaca atgattcaac
    27781 ctcgatatat cagcaaagtg gtggatcccc aaactggaga agagcgaaca actcaaacag
    27841 aagttctagg gcaacctttt tcaaaagaaa caacagaaaa agtccgtgaa tatatgagag
    27901 atgttgtcga aagcgaaaac tacggtagtg cttatggtgt atatagtgta cctggataca
    27961 atgtatcggc aaaaaccgga acagcccaaa tcgcttctga tacaggggga tatcaaacag
    28021 gagatacagc ctacctgtat tcgattgtgg agatggttcc ttctgaagat ccagactatg
    28081 tcttgtatct aacaatgaaa caccctaaga catatgatcg aatggcattg gctaaaatcg
    28141 caaatccatt gatgaaacgg gcaatggatt ttaaagaaac tgaagaagat tcagatacag
    28201 aaacaaaaac agaaaaagta tccgtagccg attatcgtaa tcttgaagcg gacgttgcag
    28261 cagctgatgc tcaaaaaagt ggactgcaac ctgtagtgat cggtaatggt aaaaaagtac
    28321 aaaaacaatc tactgccaac ggtgatcagt tgatctctgg agaaaaactg atcctatata
    28381 ctggtggtga taagctgatg cctgacgtaa caggatggtc aaaagcagat atcatgaagc
    28441 tggggaaaat cctcggtatt gaagttagtt ttgatggaga tggttattgt gtcaaacaag
    28501 aacttgctcc atatgaaaaa atcactgaag acaaacttag ttttacttta gaagaataag
    28561 aagagaaata ggagagaaaa gagcatggaa tggacacaag cattgattcc cattgtcagc
    28621 agttgtgcaa tgacaattgc cgcaatgccg ctatttattg gttattttca aatgaaaaag
    28681 caaggtcagg cgattcgtga ggagggacca aagtggcaca atagcaaagc cggaactcct
    28741 acaatgggcg gcttagtgtt tctgatcggc tctattttga caggaatttg ggttggagct
    28801 tggcaaaaac agctaactcc tactcttttc atcttgcttt ttgtattagc gttatacgga
    28861 gtaatcggct ttcttgatga ttttataaaa atattcaaga aaagaaacat gggactgaac
    28921 tcgaagcaga aattgattgg acagattcta gggggaatca tcttttatct tgtttaccgt
    28981 tctgaaggtt atccaggtgt attgaatttc ttcggactag atctaccttt aggaatcatt
    29041 tatggtattt tcgctctctt ctggcttgtt ggattttcaa atgcagtaaa tctgacagat
    29101 ggcatcgatg gattagttgc tggcttagga agtatctctt ttgcagctta cggtctaatt
    29161 gcttggcatc agcatcagta tgatgtatta atcatttgct taagtgtttt aggaggttta
    29221 ctgggtttct tcgtctacaa cagaaaacct gccaagattt ttatgggaga tgtcggttca
    29281 ttagcgttgg gcggactttt agcggcaatt tctataatgc taaaccaaga atggacattg
    29341 cttttgattg gtttgatcta tgttatggaa acagcaagtg tgatgcttca agttacttcc
    29401 tttaagctta caggaaaacg aatttttaaa atgtctccaa ttcatcatca ctttgaaatg
    29461 tgcggatggt cggagtggaa gattgataca gttttttggt tgatcagcat tgtgacttcg
    29521 ttgatcacat tatggatcgt ttggtaggag atgacgggaa atgaaaaaaa tatcaacata
    29581 tgaaaataag aaagtgcttg tcttaggact cgcaaaaagc ggagtaagtg cggccaaatt
    29641 attacatgaa ttgggcgcac ttgtcaccgt taatgatgga aaaccttttg acgaaaatcc
    29701 ggaagcacaa gagctgcttt ctttagggat aaaagtaata acaggtagtc atccaatcga
    29761 attgttggat gaagaatttt cattgatggt caaaaatcca gggattccct acagtcatcc
    29821 tttagtagca aaagcccaag aaatgggtat tccggtaatt acggaagttg agctagctta
    29881 cgaagtagcg gaatgcccaa ttattgggat tacaggaacg aacggaaaaa caacgacaac
    29941 aacgatgaca ggactactac tgaatgcagg agcagatcaa ggaattgcac gtttagcggg
    30001 taatattggg tatccagcaa gtggtgtagc gcaagaagca aaatcagaag ataaaattgt
    30061 gatggaattg tctagcttcc aactcatggg aatcacagat ttccgccctc atatagcggt
    30121 gattaccaat atctatgaag cacatattga ttatcatggt acaagaaaag aatatgtcaa
    30181 agctaaatgg aaccttcaaa aaaacatgac agaaaaagat tatctgatcc ttaactggaa
    30241 tcagtcagaa ctacaagaat tagcgcaaag aacaaaagca cgcgtactgc ctttctcgac
    30301 aaaagaagtg ttggaagacg gtgtgtatgc tgacgattac tcaatatact ataaaaaaga
    30361 aaaaataatg gaaatatctg aacttggcgt accaggtaag cataatgtag aaaatgcact
    30421 agctgctatt tctgtagcaa aattgtatgg tatatcaaat gaagctatac gagaaacgtt
    30481 gcattttttc catggcgtac cacatcgtac gcaatatgta ggagaaatcc aaggcagaaa
    30541 attttataat gattcaaaag caaccaatat tttagcgact aaaatggctc taagtggatt
    30601 tgaaacgtct aaggtcgtgt tattggctgg cggattagac agaggaaata catttgatga
    30661 attaattcct tcattaaaag gaatcaaagc aatggtcgta tttggccaaa caaaagaaaa
    30721 gttgatggat gctggaaaaa aagcaggcat cgagacaata gtaacagccg attctgtaga
    30781 acaagcagtt cctttagcat tagaaaacag tacagatggt gatgtagttt tattatcacc
    30841 agcgaatgcc agctgggatc aatacccaaa ttttgaaact agaggaaaca gattcatgga
    30901 agctgtgaac cgattaaaat agaaaaagta gaagcgagcg ataaaaaatg aaaattttgg
    30961 ttacaggcgg aggaacaggg ggacatattt atccagctct tgcctttgtc aattatgtaa
    31021 agactcaaga accgaatgct gaattcatgt atgtgggagc aaaacgaggt ctagaaaaca
    31081 aaatcgtccc tgatacagga atgcctttcc ataccttgga gattcaagga tttaaacgaa
    31141 aaatttccat gcataacatc aagacggtcc gattgtttct taagagtatc cgtcaagcaa
    31201 aaaaaatcct cagtgatttt caaccagata tcgttatcgg tactggagga tatgtttccg
    31261 gagcggtagt gtacgctgca gccaaaatgg gtattccaac agttatccat gaacaaaaca
    31321 gtgtaccagg tgttaccaat aaatttttaa gccgatatgt ggatcggatc gctctttcat
    31381 ttaaagatgc ggcttccttt tttcctgaaa acaaagcagt tcttgtaggt aatcctcgag
    31441 cacaagaagt agcagatact aaaaaatcag atattttgaa aacatttgat ttggatccag
    31501 aaaagaaaac agtcttgatt tttggcggta gtcaaggagc acttaaaatc aatcaagcag
    31561 tatcagtttt tttaagtgaa tatattcctt cttcttacca agttttgtat gcttcaggag
    31621 atagatatta tgaagagatc ctaaataaaa taaatcctga aatacaaaat attagtgtcc
    31681 agccttatat caaaaatatg gcagaggtca tggcttccag tgatctgctg gtggggcgtg
    31741 ctggtgcaac ttcgatcgca gaattcacag ctctggggtt accggcagtg ttggttccca
    31801 gcccatatgt tacgaatgat catcagacta aaaatgcaat gagtcttgtt cacgcaggag
    31861 cagcaaaaat gattgcagat aatgaactaa caggtgaatc actaagtcag acagtcaacg
    31921 aaatcatggg tgatgaagaa ttgcaaaagc aaatgtgccg agcttctaaa gaacaaggaa
    31981 tccctgatgc ttcaaaacgt ctttatgact tagtaaaaca aataatcaaa aaataaaggc
    32041 ggtggtctta gtgagtaaga aaaaagaacc aaaaaaattg tctcatccgt cttctgatga
    32101 aaacaatttg actccttggc aaaaggctaa ccgaaaatat ctggcagaac aagagatgaa
    32161 aaaccaagag ccaaaaaaag aagacataac attagataca cccctttcag attctgaaga
    32221 agaaaaaacg gttcaagagg aacagcaaga agaaagccag tccgagaaaa aaatcgaatt
    32281 ttttgaagag atcaagcaaa agaaaaacgg tggaccatat aacggttctt ttttgaaccg
    32341 gctgcccaat ttaaaaagtc aacggcacaa agtactattt cgtcggttag ctttgatcat
    32401 cactgtattg ggtataccgc tgattttttt gatctattat gtttctcctt atagcaaatt
    32461 acaagcagtt gcagtatcag gcaataagac agtcaacagc caagaaatca tctctgatac
    32521 aaaattgtca ttaggagaaa atgtttgggg acagtatttt catcgatcta cttatatcga
    32581 tcgccttaaa aaagcacagc caagaatcga aacagcaaat atacatttca aagggatgaa
    32641 tgaattcgat ttggatgtaa cagaatataa agagattgct ttgatcgcaa aaaacaatca
    32701 atactaccca gtgattgaaa atggaacagt attagatgaa aaagtagcaa atcctacaaa
    32761 aaatctccct atcttggaag agttcaaaga ctctgcaaaa atcaaagaat taacaaagca
    32821 atacaatcag ttatcatcag aattacaaaa agcaatttct gaaatcaagt acacgccaag
    32881 agcaagcaat aaaaatctga tccaattgaa tatgaatgac ggcaatcaag ttattgtaaa
    32941 tatcaataat cttgcgaacc aaatgaaata ttattcgcaa gtagccaaag atatggatga
    33001 aaaaggtgtg atcgacatgg aagttggtat cttctcgcat ccttacggta gcactcaaga
    33061 aacggaagaa tcgggccaat cggaagaaac aactgaaagt gaagaaaatg cggatcaaac
    33121 aaacgaatca agtgaagagg cagaaacgca aaaagcacaa acacctggat cgtctagcac
    33181 aaacggatca tctgattcaa atgattcaag tgaaacaagt caaatagaag ataatccaag
    33241 tacaactccg tcaagtttaa atgaaaattg ataaaacaac tttaaaaagt aagaatgtac
    33301 tttctaaaaa ccagtaattt atggtagaat tggaagcagt gaatatatct attaataagt
    33361 atattttaaa acggacgcta gttgttcgct tgtatatgga atagtaagta ggagggaatc
    33421 ccattcatgg caaaaacagg aatgtatgta ggccttgata ttggaacgac atctgttaaa
    33481 gttgtcgtgg ctgaatatat cgacagccaa atgaatatta ttggagtagg aaacgcaaaa
    33541 tcagaaggga ttaaccgagg cattatcgtt gatatcgata aaacggtcca agcgatacaa
    33601 cgagcagtac gacaagcaga agaaaaagca ggtatccaga tcaaaggcgt aagtgtcggt
    33661 ttgccagcaa acatgcttga agtagaaaat tgccagggaa tgattgcagt taacggagat
    33721 tcaaaggaaa tcaccgatga agatgtcaga aatgtggctt cagctgccct tgttcgttca
    33781 atacctcctg aacgtcaaat cgtgtcaata ttgccacaag attttactgt cgatgggttc
    33841 gagggaatca aagatcctcg tggaatgatc ggcgtaagat tagaaatgta cggtctgttg
    33901 tttacaggac ctaaaacgat catccataac atacgtaaat gcgtggaaaa tgccggatta
    33961 atcgtcaacg aaatggtgat tactccttta gcgctgactg aatcaattct ttctgacgga
    34021 gaaaaagatt ttggtacgat cgtcattgat atgggtggcg gccaaacaac aacggctgtt
    34081 atgcatgata agcaattgaa gtttaccaat cttgaccaag aaggtggcga atttgtcact
    34141 aaagatatct ctattgtatt aaatacgtca tttaacaatg cagaagcgtt gaaaataaac
    34201 tacggggatg catacccaga acggacatca ccagatgaag aatttccagt agatgtgatt
    34261 ggtcagtcag aaccagtgaa agtcgatgaa cgctacttgt cagaaattat ttcagctcga
    34321 atggaacaga ttttcaacaa agcaaaagat gcattggacc aaatcgaagc attagagttg
    34381 ccaggtggcg tcgtattgac aggcggagct gctagtcttc ctggtgttgt ggacttggca
    34441 caagaaatct tcggcgtaaa tgtaaaattg tatgttccaa atcacatggg gctgagaaac
    34501 ccggtattta caaatgtaat aagtatcgtg gattattcag caaacttgag tgaagtgtat
    34561 caattggcaa aaattgctgt tacgggtgaa acctcagcag ctcgtcaaat ggtagttgaa
    34621 caagaaacaa caaacacata tgagagctat gaagcacctg aagaaacaat ctacgatgag
    34681 ccggaaccaa aagagtccgg agaaaatgta aaaaataaaa tcaagggttt ctttacaaat
    34741 atttttgatt aattcatcct gggaggaata agatatggaa ttctcaattg ataataatat
    34801 caatgacggc gcagtaatca aagtcatcgg agtcggtggt ggcggtggaa atgctgtaaa
    34861 ccgaatgatt gaagaaaacg ttaaaggtgt tgaattcatt acagccaaca cagacgtgca
    34921 agcattaaaa aattcaaaag ctgaaacagt tatccaatta ggccccaaat atactcgtgg
    34981 tttaggtgct ggttctcagc cagaagttgg acaaaaagcg gcagaagaaa gtgaacaatc
    35041 cttacgcgaa gctttagacg gtgcagatat gattttcatc actgcaggaa tgggtggagg
    35101 aaccggtaca ggtgctgcac caatcgttgc aggtatcgcg aaagaattgg gcgcattgac
    35161 tgttggtgtc gtgacacgtc cattcacttt tgaaggaccg aaacgtggcc gttttgctgc
    35221 agaaggtatc gctcgtttga aagaaaatgt ggatacatta ttaattatct caaataaccg
    35281 tttattagaa gttgtagata aaaagacacc gatgcttgaa gctttccgtg aagcagataa
    35341 tgttttacga caaggtgtac aaggtatctc tgatttgatc acagcgccag gttacgttaa
    35401 ccttgacttt gctgacgtga aaacagtgat ggaaaaccaa ggtacagcat tgatggggat
    35461 cggtgtggct agtggagaag atcgcgtagt agaagcaacg aaaaaagcaa tctcctctcc
    35521 attgcttgaa acatctattg atggagctga acaagtactt cttaacatca caggcggttt
    35581 ggatatgaca ttgttcgaag cacaagatgc ttcagatatc gtagcaaatg cagcaactgg
    35641 cgatgtaaat atcattctag gtacatctat caatgaagaa atgggcgatg aaatccgtgt
    35701 gacggtcatc gcaacaggta tcgatgaatc aaaaaaggaa cgcaagtcta gccgaccagc
    35761 tagacaagca caaatgcaat cacctgctca aaagactgta ttagatatgg atcaagctaa
    35821 accaacttct tcagaagaag aaaacagttt tggcgattgg gatatcagac gtgaacaaaa
    35881 tgttcgtcct agagttgatg attccaattt tgacaatatc gaaaaaaaag aatttgatac
    35941 attcaaccgc gaagaaacaa aatctcatgg cgatgatgaa ttaaatacgc caccgttttt
    36001 ccgtcgtaaa aaataaagga gaggaagagc acatgattgc tgacaacttg cagaaaatca
    36061 agcaagaaat tcaggattcg tgtgctcttg ttcatcgtga tccgaaagaa gttacattag
    36121 tagctgtaac aaaatctgtt gaatccgctc aagctgaaga attggttaga gctggcgtta
    36181 tgaattgtgc agaaaaccga gtagataaat tattagaaaa gaaacaagat ttatttgatt
    36241 ttcctgaaat aaagtggcat ttaattggta atttacagcg cagaaaggta aaattgattg
    36301 taaatgaaat tgattacttt catgcgttgg acagtttaag attagcagaa gaaatccaga
    36361 agcggttaga gaaagaatta tcctgtttta ttgaagtaaa tgtttctggt gaggaatcaa
    36421 aacatggaat ttcaccagat gagcttttac cgtttgtgga atctttagct gcctgcacaa
    36481 aaatcaaggt tgttgggttg atgactatgg cgcctaaaga agcttctgat gagcaaatcc
    36541 ataaagtgtt tggtacgctg aaagaattgc gtgatcttgt tcaagcaaaa ggttatgccc
    36601 atgcaccatg tagtgaatta agtatgggga tgagtcaaga ctttcaaatt gcgattgaag
    36661 aaggtgcaac atttatccgt gtaggaacag cgctattcaa agacgaataa aggaggaaca
    36721 aaaatgccgc ttttaaacag aggagcaatt gctaattttt tcggattagc tgatgaggaa
    36781 gagtacgaat accaagatta tcctgaaaca acaaaaaagg ctgtacagcc aactaaaaaa
    36841 aatacaacac agaatcaatc aactcattct gtatctgctg cgaatactca tgaaaaacaa
    36901 aaaacggcga cttcacgtcc tgtaacgcat gaaagtattc agccggctga aaagaaaaaa
    36961 gtaattgaac tacatcaatc acctacaaaa actgtagcga aagaaagaac agcttcaaga
    37021 acctctacac aacctaaagc aaatcttgct ggaaaaatca ctgtaatgga accaagagct
    37081 tattctgaag ctatggcgat tgctaaacga atcattgctg gagaagcaac cttagtgaat
    37141 tttcatttag tagaagaaaa acaagcgaga agaatcgttg attttctaac aggaacagtt
    37201 tatgcactag atggagatat caaacgtgtt ggagatgaga tttttctttg tacaccgact
    37261 gggatggaga ttgacagttc aaccgctcaa tcttttgcag atgcgaattt gtttgatctg
    37321 taagaggagg aactacgatt tatactattg cactattact tcatttactt caacgggcag
    37381 cctatttcta tagcattatc ttgatggtat atgcattgct ctcttggttt ccaggcgggt
    37441 atcaatcagg gctaggacga ttattagcta agatctgcga accgtatatc agtttgttcg
    37501 atcgtctgcc tttgaggatt ggacccttgg atttcacaat tttaggggca atcctgatct
    37561 tgaacttagc cgtacaaggt ttagcacaga tcgttatatg gataactgtc catttagttt
    37621 attgattgaa gcaacaaaat ctttattctc agaatagata ggaggggaaa attttggatg
    37681 caaatgtata ccagcacttt cgggcagatg aacgtacatt tatcgattcg gtaggcgatt
    37741 ggattgaaca ggttcaagga caatatgcac cctatctgac cgaatttctt gacccaagac
    37801 aatcctatat tttagaaaca ctgattcgac aaagcagtga tttgcgtttc atgttttatg
    37861 gcggatatga acaggcagaa agaaaaagat gtttgatttt ccccgattat tacgaacctg
    37921 tagaggaaga ttttgaaaca gcattgtatg aaatccatta tcctagcaaa tttgccagcc
    37981 taagtcatgg gaaaatcctt ggaactttag tgggaacggg gatcaaacgc tcttactttg
    38041 gcgatatcat ctcggacggt gatcgctggc aagtatttat tgcaaatgag gtggatcatt
    38101 ttgttgctgc acaagtaaca aagattggaa aggttgctgt acgcttggaa ccaatcaaat
    38161 acacagaaat gattatgccg aaagatagct ggacacaaga aagaggaaca gttagttcca
    38221 tgcgactaga cagtgtgatc tctgagattt ataatatttc cagacagcgg tccaagcagc
    38281 tgatcgaagc tggtaaagta aaagtgaact ggacagaaaa tacaagacct gattttgtac
    38341 ttgaactatt agatattatt tcagtcagag gttatggcag agtccagatc caagaacttg
    38401 aggggaagac gaaaaaagaa aagtacaggg tactgttcgg tgtattacgt aaataaaata
    38461 aaaaggtggt ttactcatgg ctttaactcc attagatatc caaaataaga cattcccgac
    38521 aaaaatgcgg ggatataatc aagacgaagt agatgatttc ttagatttag ttgttcgtga
    38581 ctatgaggaa ttgactcaaa gaaatcgaga attagaaaaa gcagtcaaac attctgaaga
    38641 aaaacttgag tacttcaatg aattaaaaga tgctttgaac caatcaatca tcgtagctca
    38701 agatacggct gataaagtaa aaacaagtgc aagcaaggaa tccgaagtga tcgttacttc
    38761 tgcacaaaac aaagcagatg agttagttgc gaacgctgaa aaacgtgcac atcaattaac
    38821 aactgatgca gaagaaaaag caagaaaaat cttgacagat gcaacagaaa aagcaagaca
    38881 gcttgcgaca gaaactgaag atcttaagaa aaaaacacga gtattccatc aacgtatcag
    38941 cttgatgctt gagtctcaat tagaacaagt gaaaagtccg gaatgggatg aaattttaca
    39001 accattctcc agctacgtga ctgacagcca tgaagtgatc aaagaagttt tagcaaaaca
    39061 gcttgacaat gaaaatgata ctgatgtaaa ctcagacaca ggagtcatag aagcacctat
    39121 gacggcattt gatacacagg ctattgatat ctcgatcgtg cctggtgaaa atgaaaacga
    39181 gtagaacaga aaagcacttt gttattctta aagcgagccg atgagagtgg aagttcggta
    39241 agacactgca tggtgaaaga tcctctactt gagacctgct tttccgaaat tagagtaaga
    39301 agcagcggct gacctcgtta ttggtcacag agagtttgca ctagatagat gcaactaaat
    39361 ttgggtggta ccacggcatg ttcgtccctt gcggctgaac atgctttttt gtgtccggaa
    39421 aacaaataaa ggagtgtatg gttgaatgaa aatgaaagaa accttgcaat taggaaaaac
    39481 aaaattccct atgcgtggta atttgccaaa ccgagaaggc gagtggcaaa aagaatggga
    39541 agaaaatcga atctaccaaa aaagacaaga gttgaacgaa ggaaaaccta gctttgttct
    39601 tcatgatgga cctccatatg ccaatggtaa tatccattta ggacattcat tgaataaaat
    39661 cagtaaagac attatcattc gttcaaaatc aatgtctggc tatcgtgcac catatgttcc
    39721 aggatgggat acacatggat tgccgattga acaagtattg gctaataaag gcgtgaaacg
    39781 taaagaaatg tccatggcag aatacttgga aaaatgtcgt gaatatgcac tttcccaagt
    39841 agataagcaa cgagcagatt tcaaacgcct aggagttgca ggagattggg aacatcctta
    39901 tgttacatta gatccttctt atgaagctgc acaagtacgc gtatttggta aaatggctga
    39961 aaaaggctat atttacaaag gattgaaacc aatttactgg tctccgtcaa gtgagtcttc
    40021 tttagcggaa gctgaaattg aatataaaga tgtaaaatct ccatctattt ttgtagcttt
    40081 caatgtcaaa gacggcaaag atattcttga tgaagataca gcttttgtca tttggacgac
    40141 aacaccatgg acattgccag caaaccaagg tattgctgta aatccaaaat ttacgtatgt
    40201 attggtggaa gccgatggcc gtaagtttgt cgtagcgaaa gatttgctag aaacagtcca
    40261 atcagaaatc ggttgggaaa atgtaacagt tctaaaagaa tttgctggac aagaaatgga
    40321 atatatgact gcaagtcatc cattctatga acgtgaatca cttgtgattt taggcgatca
    40381 tgttacttta gacgctggta ctggattagt ccatactgct ccaggacatg gggaagatga
    40441 ctatatcgct ggtagcaaat ataaactatc tgtagtgtca cctgtagact caaaaggatt
    40501 gtttacagaa gaagcacctg gatttgaagg tatcttttat gacaaagcaa atcctatgat
    40561 cactgatcta ttaaaagaaa aaggagcttt gctgaagcta gatttcttta ctcatagtta
    40621 tcctcacgat tggcggacga aaaagcctgt gatctatcgg gcaactccac aatggtttgc
    40681 atctatcgat aaattccgtc agaatatttt ggatgaagta gaaaaagtag agtgggttat
    40741 cccatggggt aaaacacgtt tgtataatat gatccgtgac cgcggcgatt gggtaatttc
    40801 ccgtcagcgt gcatggggag taccacttcc gattttctat gctgaaaatg gagaagcaat
    40861 catcacacca gaaacaatcg aacatgtggc aaaattattt gctgaacatg gatcaattat
    40921 ctggtttgaa aaagaagcga aagaacttct tcctgaagga tttacccacc caggttctcc
    40981 taatggcgaa tttacaaaag aaaaagatat catggatgtc tggtttgatt ctggttcttc
    41041 tcatgaagct gttttacgtc agcgaccaga attatcattc ccagcagata tgtatttaga
    41101 aggatcagac caatatcgtg gctggttcaa ctcaagtatc acgactagtg tggctatcaa
    41161 tggtgtagca ccatacaaat cagttatttc tcaaggattt gctttagatg gtgaaggccg
    41221 taagatgagt aaatctttag gaaacatcat tgcaccagaa aaagtgatca aacaatttgg
    41281 tgcagatatc cttcgcttat gggtatctag tgtagattat gaagcagatg ttcgtgtttc
    41341 tatggatatc ttaagtcaag tggcagaagt atatcgtaaa atcagaaata cgatgcgttt
    41401 cttattagcg aatacagaag atttcgatcc taaaaaagat actgtttcgt acaatgattt
    41461 acgttctgta gacaaatata tgaccgttcg attgaaccaa gtaatcaaag aaatccgaga
    41521 agaagggtac gacaaatata acttcatgca tatttaccgt acagttatga acttcttgac
    41581 ggttgatcta tcgtcattct acctggattt tgctaaagat gtagtttata ttgaagcaga
    41641 agatgattat caacgtcgtt gcatgcaaac tgttttctat caaacagctg tagctttgac
    41701 aaaattattg actcctatca ttccgcatac agcagaagaa atctggtcat tcctgaaaga
    41761 agaagaggag tacgttcagt taagcgagtt tcctggttat gaagagtttg caaaccaaga
    41821 agaattgatg gatacctgga ctgctttcat ggacttccgt gataatgtct taaaagcatt
    41881 ggaagaagca agaaacagca aattgattgg taaatctttg gaagcaaaac taactgttta
    41941 tccaaaacaa caagttcgtg aaatgttaaa agctttggat gcagatattg cacaattatt
    42001 gatcgtttca ccagactact ttgaagttat ggcagcagat gaacaagtgc cagataacgc
    42061 tatggtattt gatgatgttg ctattacagt ggaaaaagca gacggggaaa cttgcgatcg
    42121 ctgtcgccaa gttcgtaaag atgtaggagt agatgaaaaa cttcctcatc tatgcggtcg
    42181 ttgtgcaaaa atcgttgaag acttctatcc tgaagctgta gctgaaggat tcgaagaaaa
    42241 gtaaaaggga aaagggggtg tgacatgttc tttgtcacac ccccttttgt tatcaatcgt
    42301 ttaattttcc acgttcacga taccagttat ctggttgcca gatccattcg aatccgtctt
    42361 tttcaagtag ttcgaaagct gctttaggtc ccattgatcc agctttgtag ttgggaaaat
    42421 ccggttgtgt ttggtcccaa gcttttcgaa tgacatccac gatatgccaa gattgagcga
    42481 cttcttccca atgagagaag ttcgttccat caccattcaa cgcatctaat agtaattttt
    42541 cgtaagcttc tgggctattt tcaaccattt ctgcactatt tctaaattcc aatttgattg
    42601 gttccgtttc aaatccttgc cctacttctt tcccattcaa ggataaagaa aatccttcag
    42661 ttggctgaat atagatcgtt agaacattag gtggaagtgt cgtatcgtca catggttcat
    42721 cgacagatgt tttgaataca ttaacaggaa cttgtttgaa tacgatgttg atccgagtcc
    42781 ctttttcagt caaacgtttg cctgtacgta catagaaagg aacacctgac caacggaagt
    42841 tatcgatagt gaatttgcct gcgacgaatg tttcagtctc tgattgagca tccactttgt
    42901 cttcttcgcg atagcccttg aattgtacac catccaattc accttgagca tattgcccgc
    42961 gaacgaagtt ttctaacact tcttcttcgt tataaatgcg gatagctttc aatgctttga
    43021 tcttttctgt acggatttct ttttcggaaa atgctactgg cggttccatt gcaagtaatg
    43081 ctacaacctg taaaatatga ttttgcacca tgtctttaag tgcgccacta tgatcgtagt
    43141 agcccccgcg atcttctaca ccgaggtttt cgccaaaagt aatttggata ttgtcgatat
    43201 agcgattgtt ccattgagcc tcaaaaatat tattggcaaa acggatagct gagatgtttt
    43261 ggatcatttc ttttcctaaa taatgatcaa tcctgaaaat atcttgttct gggaatactt
    43321 ctctgatttg ctggttcaat tcaaaagcag actggtaatc agaaccgaac ggtttttcta
    43381 tgatcaaacg attgaagcca ttttttgtca taatattttg tgatttcaaa tgagaaacga
    43441 tcgttccaaa aaattgaggt gccatagcca aataatagac acggttaccg tttaattgat
    43501 agtgtccatc taatcggtca gacaattctt ttaatgtctg ataatgcgtt gaatcattta
    43561 catcatggga ttgatagtaa aaatggcttg caaaggtagt tgcttcttca tctgaaggat
    43621 tcaatgcacg gattgtttct ttgacaactt cacggtagtg ctcatcgctc cagggacggc
    43681 gagcggttcc gattacagca aaatgctcgc ctaaatttcc ttttctgtaa aggcggaaca
    43741 aagacgggta caatttacgc tgggctaggt cacctgtacc gccgaatatc gtaaacaata
    43801 catttttttc attagacatg tggatacact cctttatatt ttatcgacaa aaatcgtgct
    43861 ggccgcctta aagctgacgg ggattttctt tctatcattt tggatagtga tcggtccttc
    43921 ataagctgcg atgtcaacga tctcatattc ttcgttgata ttcaggtcaa tagtgactaa
    43981 gtaatccaac aattcttttt catcaagcac tcgtgcgata cgcacttttg taccgacagg
    44041 ataggactcc aatgtttgtc ggcgttcttc atggacaact tcctcttttt taggaatcga
    44101 tcctccatga ggacaatgtt caggatgatt caaatagttt tctaaatgat ctgcaagatg
    44161 ttccgaagtc acatgttcca atacttcagc atcatcatgg acttcattcc agctatagtc
    44221 taaatgttca actaaaaaaa cttcccataa gcgatgtttg cgaatcagac tgctagcttt
    44281 ttgcagtccg cttgcagtca attgtacacc ttgataaggg gaatgttcta caagctcttc
    44341 tttcacaagt ttagaaatca tttcactgac agatgcagct gagacatcta gactagatac
    44401 gatttgttta ttgttgactt tcgttgtatc tccacctagt tctaaaatga ttttcaaata
    44461 atcttctcga tttggtgtca tattcgatca tcctttcatg gtaaaaaacc atcattagta
    44521 agtgtaacaa aaaaaacgtt aattgactat ttttctaaat aaaaaacaaa ggatttttag
    44581 acgtttttac aacaaaaaac gaaagatgat agagaagact cattctttcg tttataaagg
    44641 atatatatat acattgggag ggatctattt ttcagataac aacttatatg aagtgtctgc
    44701 agattcgtca taaagagttt gttcgggata agaaaagata agataaaaac catactgatc
    44761 acgtacgact aaacgatatt catctatttc taccacagat gtgccaagag actgaccgtt
    44821 gatcaaaaac gtgtaatcag atgtaatctg taattgttgt ttttgctggc tatcactaaa
    44881 gaaccatgta ccttgatact ttgagagtcc ttctgacttt tccgcttgtt ttttcttttt
    44941 caagtaaata aaacttgaga gaacggtaat gagtgaacca agaaaataga tcattttttt
    45001 tatcaccaaa tcactccttt atgagaaaat aatcgcatag acatattctt tcttctttat
    45061 ttcattgtac cgaataaaag ctaaaatttc tctaagactt tcgactggtt tttttactgt
    45121 ttttctacaa attatgatag gatacatagt aggaagaaag gtgacataat ttgaagtatc
    45181 agcaagtgga aatagctttt tttattgacc gcccgaatcg gtttatagca cattgtatga
    45241 aaaaagacgg agaaataatt attgcgcatg tcaaaaatac aggtagagga aaagaggtcc
    45301 tgataccagg cgctgaagtg gctattgtat ttgctcctgg acctaaacgg aaaaccgcct
    45361 atgatctgat tgctgtaaaa aagaagggtg actggtttaa tatcgatagt caattgccta
    45421 atcggctggc aattgatggt attttagacg gcacgatcct tttaccagga ttaactggtc
    45481 catttacggt atacaagcgt gaagtaactt atcttcactc caaatttgat atttatttag
    45541 agacggtggt cggacaaaaa gcatttgttg aagtcaaagg tatgacactt gaaaataaag
    45601 cgattggtgc ttttcctgat gcgcccacac tcagaggatt gaaacatata ggagaattaa
    45661 cgtatgccgc acaagatggt tatgccgctt atgtcttgtt tattgctcag ttcgaacatc
    45721 ttcatctggc aacgatccat gaagaaatgc aaccagcact agctgatatg gttcgacatg
    45781 cgcagcaaag cggagttcaa atcctcgcct ataattgtca agtatcggca gatgaggtaa
    45841 ctgtcaagca ggccattcca tttgatgtag atatcccttt tgaagatcct atacaataga
    45901 aagaaggtaa aataatgatc aacttaggta ttatcggaac aaattggatt actcatcaat
    45961 ttgtccaagc agctttaagc acaaaaaaat atgaattgac agcggtatat tctagacatt
    46021 tggaaaaagc acaggaattt ggttctcatt atgaagggga tatcgaattc gcaacagatt
    46081 tgaagacttt tttcaatatt actcatatgg acaccattta tattgcttct ccgaatagtc
    46141 ttcactttag tcaagctaag caagcaattt tggcaggaaa aaatgtgata gttgaaaagc
    46201 cagctttttc gacgccaaaa gaaatggatg aaatcatcca tttggcaaat gaacacaatg
    46261 tgtatttttt tgaagcagca agaaatatcc atgaatcagg ttttaaaaag attgccgatt
    46321 acttaccatt aaaagacgag ataatcggtg caaatttcag ctatatgaaa tattcttcgc
    46381 gctatgacca agtattagat ggagaagaac cgaatatctt ctcgactcat ttctcaggtg
    46441 gtgcattgat ggatttaggt gtttaccttg tatatgcagc tgttgcttgg tttggaatgc
    46501 caaaagaagt ccattatttc ccaagaaaga tttctactgg tgtagatggt cttggatggg
    46561 ggatcttacg ttatgagaat tttgatgtat cgatccagcc aggtaaaatc ggtgattctt
    46621 atttaccgtc agagatttat tttgactcag gaacgctagt gatgaatgga gtcaatgcaa
    46681 ttgaacaagc acaatttcat gatcgcgcgc ataaagaaat cattgatttg gcggtaacag
    46741 ctgcagaaaa tccaatggca gatgaagcag aggattttgc caatgtcatg gctcatccga
    46801 aagatccagc ttggggattg ttgtatgaag agtggatcga gctagctaga aatgtgaacc
    46861 aagtgattta tgatttaaga aaaaatggcg gcatcaaatt cgatgcagat aatgaggaag
    46921 atttttaatg aacgaatttg tacaagtatt tcccgaaaat tggcagaaaa aatatgagga
    46981 taaagggttt atctcacctt ctcttattca acgagcagtt tttcagccac taagtaataa
    47041 acaaagtata gtagcggttt ctcctacagg ttcaggtaaa acactggctt atctttggcc
    47101 tctactacta aacgtggaac caggtgaagc aagtgcgttg gtcatttttg cttcttctca
    47161 agaactagct attcaagtag cagatgttgc acgtgaatgg ggaaaagaca aagagttgaa
    47221 agtccaatct ttggtgggcg gagccaacgt caaacgtcag attgagggtt tgaaggaaaa
    47281 accagaaatc ttgattggta caccaggaag aatattggaa ttgatgaaag caaaaaaaat
    47341 aaaagcacat caagtgaaaa caatggtatt tgatgaagca gaccagctat ttgacgctgg
    47401 gaacagccaa atcattgatc aaattcttca tcaagctcca acagagtatc agctagcttt
    47461 tttttcagcg acagcagatc gttcccttga aagcattgaa aaaatcactg ggaaaacgtt
    47521 tgagacaatt gacgtgacag cagaggacga ttctcgaaaa ggactgagac attacttttt
    47581 acgtgtaccg atacgaaaga aagatgacta cttgagacgt ctcacgcata tagaagattt
    47641 tcatggtctg attttcttta atcaactgac tgaattaggc gtaatggaag aaaaattgct
    47701 gtatagaggt gtaccggtag gaagtctggc ctctgatcag aacaagctac tccgaaaagc
    47761 agcgctagaa caattcaaaa agaaaatggt cagagcactt ttgaccacag atgtggcggc
    47821 tagaggttta gatattacag gtcttcctta tgtagtgaat gcagaagttc cgttaagtga
    47881 agaagcttat ttgcatcgtt cgggaagaac tggtcgaatg ggtaatgaag gaatagtaat
    47941 tacactcgta caagataata atctaagaga tttaaaacgg cttttacgtc catcaaatat
    48001 aacacttgaa gagatttttt tatacggagg taagctgcaa actgagcagc caaccaaaga
    48061 agatgcagct cctgcagtaa ataaaaagta tgcgaaaact ttttctggaa aggagcagaa
    48121 cccagaatat aacaaagaaa aaataagaaa aaaagttaaa aataaatcaa aaaaagagag
    48181 aaataaagga gcaaggcgca aataagcgcc tttttatgct atgattatga aaacgggaac
    48241 aaaattggtt gccagaggag gggttatgtg aaagaacgtc aagaagagat cttgcagcta
    48301 ttgacagaaa accaacaagg attgacagca agtgatgtag ctgagcgctt aactatcgat
    48361 cgaagcaatg caagccgata tttaagtgaa ctatacaagg cccatcatat tgtaaaaacc
    48421 gctggccgac cagtcgttta ctctttgcca acggaaaagt caaaatctga tgaagtgcat
    48481 gtcgactctt ctacgcaagt gacttttgaa acactcgttg gagaaaatga ttctttaaaa
    48541 gtcagcatac agcaggcaaa agctgcgatt ctttatccac cgcgggggtt gcatacgatt
    48601 atttttggag aaactggtac agggaagtcg atgtttgctg aatgtatgta tcattttgca
    48661 atcgattcag agatgctttc tgcagatgcg ccatttgttt ctttcaattg tgcggattat
    48721 gctcaaaatc cgcaactgct ctttggacac atctttggga tcaaaaaagg ggcttacaca
    48781 ggagcagctc aagatagtcc cggtctaata gccaaagcag acggcggtat attgttttta
    48841 gatgagatcc accgtttacc gccggaaggg caggaaatgc tgtttacttt cattgataaa
    48901 ggtatttacc gtccgcttgg agaaagtgca caagtacatg aagcatctgt tcagatcatt
    48961 ggggcgacga ctgaatcttc agaaagtttc ttatctacgt tcaatcgacg gattcctatg
    49021 gcaatcacct tgccgaatct tgcttcacgt tcactggatg agcgttatga aatcatctct
    49081 ctttttatca aacaagaagc gaatcgtttg aatcaacgta tcgatatcga aaaagaagcg
    49141 attcttgcct tcatgcttta tgatgcggaa ggaaatatcg gtcaagtgaa acgagacttg
    49201 aaactagtct gtgcaaaatc ttttttacat taccgtactc atcatgagaa aaagctaatc
    49261 attcgaaaag aagaactttc ccttcaagta caaaaaggat tactgaaaat caaggaaata
    49321 ccggaacgtc tcgaccgatt catggacagc aaaagtcagt atttgacatt tgaaccagga
    49381 tttgctgatg ttgtctggtc acaagatcca gaacgaaaca tgcaggtcta taacgatatt
    49441 gaggaaaaag tcacttctct ttccgaaacc ggtgtggaga acattgatct agagaccctc
    49501 atttcaaaag atgtggatgc ttatttccag acatatgtca aagaattgac taaaagttcg
    49561 attcccaaag atcttttgcc agatgaaatc tggcggttaa ccaatcaatt gtatgatgtg
    49621 gctgaaaaac ggttagaccg tatttataat gaaaaagctc gttttgcttt tgctttgcat
    49681 ctgcaaagta cattggatcg tgtgagagaa ggtcatatga tcgtccaccc agatttgaat
    49741 aacgtacgga aaaattttaa gagggaattc caggtagcaa tcgatctttc aacgatcatt
    49801 gaagaagaag aacaagttga gattcctttt gatgaaatcg gctttattag tatgtttctt
    49861 tctatcaatc taggagaaag tgagcctgtt aaagaaaaca aagtagatgt tctcatattg
    49921 atgcacggca gacagactgc ttcaagcatg ttggagatgg cacaagaact attaggaaca
    49981 aaagtaggga aagcttttaa tatgccactg gttatggaag ttcaaacaat gtatgaagaa
    50041 gtgttacaat atgtcaaaaa ctatcaagag catttgacga atggcctact cttgttaaca
    50101 gatatgggat cattaaacac cttcagtgat ttgatttatg aagagactgg cattcggacg
    50161 aaagctatca cgatgacaag taccatgatt gtgatagaag cgatacggat ggcaagtgtt
    50221 ggaagaagct tagaagatat ttatcaaaat atccagcttt cctttgaatc gatcgtacgt
    50281 gatcagttca aagcggaaaa gtctgaaact ttacagcgta aaaaagccgt aatcgtgaca
    50341 tgctttactg gtgaaggagt agctgccaaa ttatatcagc gtattatgcc agtgattaat
    50401 caaaataagg tagaactgat ccagatgcag tttttagaac gtgaatcatt caaaaaacac
    50461 atcgatgcct tattggaaga atatgagatc aaagcaattg cgggaacagt tgatgttgat
    50521 tatcaaaata tcccattttt ttcagcctat gacgtttttg atgatgaaaa actaaatatt
    50581 ttaaagcgta tagctgatga tgagatcccg acagctaaaa ttgtcgattc acttgctgga
    50641 tcattaagcg atgtcttttc tattgatgaa ctgatccgtt cattgcaaaa gacagttcag
    50701 cagatccaga ccgatttgtc gattatcgtg gaacctggag tagatactgg aatcgttatc
    50761 catttagcct tcatgttaga cggattgatc aaacaagaaa gccatcggca atttgagaaa
    50821 ctgtctattt tctcaaaaat ttatcgtttg gaaatggata tcgttcgggc aaacttgatg
    50881 gcactagaac gaaaatatca ggtaaaaatt ccagaagatg atatcgcatt tcttacacaa
    50941 atgtttcttg aaaataaaat aaaaaaatga acacactaat catactgttt acacactaaa
    51001 catctgtgtg cgcactataa aaaagagcta aaaagcatga aaacgctatt tcagctcttt
    51061 tttttattgg catgcttctt gcgttatata aaagtgtaaa aaaataaaag gagtgttaaa
    51121 aatggcaaaa aaaacaatca tgttagtttg ttcagcaggg atgagtacga gtttattggt
    51181 cacaaaaatg caaaaagcag cagaagaaaa agggatggaa gccgatattt ttgcggtctc
    51241 tgcctcagat gcagataata atttagaatc taagaatgtc gatgtattat tattaggtcc
    51301 tcaagtacgt ttcatgaaag ctcaatttga acaaaaactt gcgcctaaag gtattccatt
    51361 agatgtcatc aatatgcaag actatggcat gatgaacgga gaaaaagttc ttgcacaagc
    51421 agaaaatcta atgaaataaa tcaagtatgc agttgggggt ataacaacgt ggaagatcaa
    51481 caaaatctag aagccatcat ggggttaatc atgtttggcg gtaatgcaaa gagcgatgca
    51541 atggaagcga ttgcggctgc taaaatcggt gattttgagt tagcggatag gaaaatcgct
    51601 gatgcagaag aatcacttat acaagcgcat catgcacaaa cagacatgtt gacacaagaa
    51661 gcacaaggaa atcatatgca agtgacatta ttgactgttc acagtcaaga tcacttaatg
    51721 acatctattg cgtttactga tttagcaaaa gaaatcatcg aactttatcg tcgcgtatac
    51781 agcgaataaa tacaacttct tttttgaacg gaagaagaaa aatttttgaa cggatttttt
    51841 attttgttat aaggagaact ctctttatat aaaaaactta taggaggaag taaaaaatga
    51901 acggactatc aagttggatg gagaaataca tcctgccggt tgccgcaaaa atcggagcgc
    51961 aaaaacattt agttgctttg cgtgatgctt ttatcggcac actgcccgca acaatggcgg
    52021 gatcagtcgc tgtaatgatc aatgcaatta ttcgtgattt accgcaacag tttaacagta
    52081 cctatgctga cagtttagca gcagacaaag gaatctttgc cgtattgaat gtcatcatcg
    52141 gaatcaatgg ttttgtctgg aacggtacac tagcaatcgc cggattgatc tttgcctttt
    52201 cttgggggta taatcttgcc cgagcttatg gagtaaatga tctagctggg gggatcgttg
    52261 gtttagctac cttgattcaa ggtatttcct ttggtccagc attatcagca gcattagatg
    52321 tgaatgttcc ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt gcaacgattg
    52381 cagatggcac actgtcagtt ggattatggg gttggctaaa actagatcat ttgaatggaa
    52441 acgcttattt tacagttatg atcatgggag ctatttcagt cattattttt tgtaaattga
    52501 tgcttgccaa cattacgatc aaaatgcctg attcagtacc accagcagta tctaaagcat
    52561 ttgcagccat tttaccagcg acgatcgctt tatacgtcat tgctattatc aattatatcg
    52621 taggtcgtgt agctgatggg caactgatca ttgatttggt gcaaaaatat attgcagaac
    52681 cattcctagg attatctcaa gggttgggag cagtattgat cgtcacagta tttgttcaag
    52741 tattctggtt ctttggtatt catggaccaa atgtattggc accagtgcta gagggaatct
    52801 ggggacaagc acaactaatc aatattgata ttttccaaaa aggttataac ggaaaaactg
    52861 gtacaccagc cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg gtccgcggat
    52921 cgttcgatgc ttttgcatgg tttggcggat caggcggaac gatcgtctta ctgatagcta
    52981 tcttgatttt ctctaaacgt gcagattatc taacagtagc taaattatcc attgggcctg
    53041 gtatcttcaa tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg aatgcaatca
    53101 tgtttatccc attcttgatt gctccagtag tagcaacagc aattggatgg acagctactt
    53161 atcttggcct agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg ccgttcttat
    53221 tgtcattctt agcaacagga gcggattggc gggcaccgat cgtaacatta gtatgtatgg
    53281 tcgtgacttt cttgatttgg gcaccatttg tcatctctgc aaacaaaatg gaccctgccg
    53341 caggcgaaca acaataagat gaacgtgagg agcgggacgc tttaagtttc gttcctcata
    53401 atgaattttc gaggaggaac tgaaatgatt acaattgcca gtgcaatgca acaagatgaa
    53461 atcaaagaat tgttagaagg atacaatgaa gaaggaatga cttttacatt taaagaaaaa
    53521 caaggaatca agctgttctt tgaaacaagt gcagaagatc cggaagttgc ggctaaaaaa
    53581 gcaaaggaac taatcaaagc acagccttgg ggaacagtcc tttattttca atcaacagct
    53641 gtttagaaaa tggtttaaaa agataggatg gttgagatga taaaaaacgg tttattttta
    53701 tgtagtattg gttctttgat cgtgttgtat agtttgaatc cacattcgca aaggtatgat
    53761 ttgtatagta tggtcacagg tctttttcta acaggattag gaatctattt cttcatgaag
    53821 gggaaaaaaa gagaagaagc aaaggagaaa aataactaat ggaaatcacg aaaaaattaa
    53881 atattccagc atcgtttttt tacgataaag ttatggactc tgtccttttc gatgtacgta
    53941 aagcgaccgg caaatcagta actagaaaac agctgaaaaa tttggagtac gtcaaacaat
    54001 tctcgcaaaa taaccgggct cgcataaaaa tcgaagaagt cattgaaaac cagtcttata
    54061 agtttcgtac atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc aaaccattgg
    54121 acgattcgac ctgtgaggtt cattacacag aaaaaatgga gtcttatggc atgcttcaaa
    54181 agttgaatga tatgctttta ggaacaatct tgatgtattt taaacggaga cgtttcaaaa
    54241 aaatgctaga tatgatcgaa gcaacgtatt aaatcaatga aacatggtac tatttagtta
    54301 ttgaaaaatg aagtaagcta gaaggaattt ttcataggaa gatagatctt tcctgcgaat
    54361 cctcactagc ttttttgtag gaaggggcag caaaatgaca aacaaagtaa gagagctagg
    54421 tgtatcttcg attgaggaaa tgtttgattt agccacttat gcatttaatg gggcagatac
    54481 acctgaaagg cgagaacgtt ttcgtacact tgcggagaat tcttggaatt acggattttt
    54541 tgatgaggag gatcggctaa caagtcaagt catggcgacc ccttttccag tgaattttta
    54601 cggacaagag tacctaatgg ccggcattgg ctatgttgct tcttatccag aagcaagggg
    54661 acaaggcggg atcaatcaga tcatggaaaa gatccttgaa gattgccgtg atcgtaaagt
    54721 aagcttatct tatctggcgc cattctccta tcccttctat cggcgctatg gctatgaaca
    54781 atcatttgat aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta tcaaaaaaaa
    54841 atctggaaaa ataaagcgta tggattttga agatgcaaaa gctgcaatca gaagaattta
    54901 tgaacagatg ccggaaaacc aacgagctgg tctgaagcgc gaagactggt ggtatacgta
    54961 taaattcaag caaaaaggca attaccagtt tgctttgcat tttgatgata atgaggaagc
    55021 aacgggatat atcgtttatc agcttgcaac gtcttccttt attattgcgg agtggggatt
    55081 tttggatcat gatgcttttt gcagccttgt tcgcttcgta agttctcaca atggtgcttt
    55141 tgaaacattt gaatattcat gcggctacgg cggtaatgat caaaattatc tattagaaaa
    55201 tccatttgct tctgtaatga tcacacctta tatgatggcg aggatcgtag atatttcttt
    55261 gtttttggaa aattatccat ttaaaaagag acaagctgca tttgccctac aaatagaaga
    55321 agatcattat gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa atggaaaaaa
    55381 tcatctctca aaagtagaac aaacaaaact cccaatcgtt cgaggaagta tccaacatat
    55441 cacgcagcta ttattagggt atcgtaaagt ggaagagttg gtattccaag aaagaataca
    55501 ggtgacaaaa gaatatgaag aaacgttaag ctcaatcttg ccacagcaac agccaattct
    55561 taatgactat ttttgataaa aaacgggtga tgacaatatt tttgtcacca cccttttgtt
    55621 tttcatttgg catttgaagg aaaatgttaa agtaaatcct cttttttcgt aaatttgaat
    55681 tttttgtttt cttttgtttt agggttttct tttttgtggt cttggattgc ttctttcgta
    55741 atcttttccc gatttttttt agatgaatct aagcgttctg cgtatacttt ttcttttggc
    55801 ttatcattca ttggaaagga cctccctaca ttatttcatt ctacctctag tttatcatct
    55861 gttaaggact agtggaaacg ttttgcctaa cttataagct gattttttta gcagataagc
    55921 ttgcaagtaa aaaacaaaca agctacaatt gatttattga aattgttcct gtagctcagt
    55981 aggatagagc aatcgcctcc taagcgatag gtcgctggtt caattccagt caggaacgta
    56041 taaataaccc ctgtatcctt tgcggtacag gggtttgttt tgtatttgtg ccaaaaatgt
    56101 gccaaactag ttatcatttt tatataaact atcgaacaca gtattgattt tttctttatc
    56161 ctgatcttca agctctcgaa caatatgaag ataagtagac attgtagttt ctaaactgct
    56221 atgtcctaaa cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc
    56281 atgtgtatgc cttagtccat gcaaagtaaa ttgcttttca atccctaatt cttttaattt
    56341 tttgcgcaaa tatttgctga cggcgtttga tgaaactaat ccatttttta agttgaaaaa
    56401 aacaaaatta tgagggtttt tgatttcaaa attctcgaat aattcttttt gattgatttt
    56461 aaactttttt aagagatcaa tcgtatgttt atcaatggaa attttcctat ttgaagtttc
    56521 gttttttgtt tttccccatt catttaattt ataattccat gttttattaa tagtgatgat
    56581 ttgatcttcg aaatcgatat cttcccatgt taatcctaga agttcagcaa atcgcattcc
    56641 agtggcacca gcaactaaca cgagcatagg agaagagtac tgcgcagaaa ggttttcttc
    56701 tacgagtttc attaaagatt taaattgatc ataatctaaa tatttatctt cttttggctt
    56761 caatgaatct tttcctttga ttactgcttt tctagttgga tcaaacggaa ttaatccttc
    56821 ttctacggca tctttcaacg acgctctaat atggttatta aacttaataa cggtagattt
    56881 tacatggtct ttagcatact tatttaaaaa gcgttgatat cccactctat ctaaatcaga
    56941 aatcaatacc gctggcatat atttctttat gttcattaac gtatcttcgt attttctata
    57001 agtgatagga gatactgttc cttctttata aagttgcatc caatcctcaa agtaatctga
    57061 taataataga tttttgcgat cccctttaag gcctttgttc aagttatatt ctaattcgtt
    57121 ggcggcatct tttgcttctc cttttgtctt aaatcctgat tttcttatct tcgtgtattt
    57181 tccgtcatct tttttgtatg aaatttcgta ttgccaacta tttccacgtt tgactaatct
    57241 tgccataatt gaatcagctc tctttctctg atacaatagg aactataaag aagcccattg
    57301 tttgggtttt attatttttt gcacatcctt tttgcttgcc ggcgcaagga tgtgctattt
    57361 gtcgttttaa tggtataatt ggatgtgtat tagatcatcc aatcagaggt gtttattatg
    57421 attaaagtga tttttaaaag aataactgaa cttccagagt ttagattatt tgttgattat
    57481 tggtatgtct ggttttttat tgttgcattg ctgataggtc tagatgtcat attagaaaga
    57541 aaacttaagt aagagttgag ttaataaatt gaattcctaa atgagatatt ttgtagccca
    57601 tattttgttt gccgacatca cctaatacta tggagctaat cgttttttct aacagaccaa
    57661 aatcagataa tctatttaag ctaccatcta ttagctcttg tgaatagcca gacatgctga
    57721 tatttccagt gaaattttcg tttccacgac tcattaagca tgctaacaat tgaatctcaa
    57781 tcgatgttaa ttgattcagt acaagaagat aatactcctc gttatcccat gaagcgttgt
    57841 taattcgaag taaacaattc ttataaagat ttttatacat atctatttta ttttgtgcct
    57901 ttgatttttc tacctcgtca tttatccctt cgaatattcc aatgagttgg tccttgttct
    57961 ctaagttgaa aacatcttct ggaatttgat cgcgaatgtt ttctaatgat tcgtttaaat
    58021 ttttgtagaa tttctcgatg cgtttgaaac gcttctcgtt tttagaccca aaataaagtg
    58081 actgcacaac gccaccaata taaggaattg ctccaatacc gccttcaact attaattcaa
    58141 gatattcttt tttggttagt ttttcattgc tattatccat agttacctcc tattttgtca
    58201 tttcctttac aagcattgtg actcctgatg gcactctttt gtataggttt tattattgtg
    58261 cacaatctaa ctcttagtgg ggcgtggttc atttattttt tacttacttt actattaatc
    58321 atatcccatt gagcatagga tttttctatg ttcgatcctc ttgaactaat ttctcctgta
    58381 aaagttactt tatcgccctt tgtaaaggta tcagcattgt ttaagtcctt cgcaaaaatt
    58441 acatatgaaa ctttaggtgt tgcagaaata tcattccaac ttttatcatc gtactctttg
    58501 tctgctataa tggctattct agtagtcatt gattcgataa cagttccttc aaaagtatat
    58561 gttttaccac taatatattt agtaaaagct gtattttgct cagtagctgg agtaatagaa
    58621 taataactat ctaagaaagc accccagtca tgattatttg tctctaaaaa ttcttcaaat
    58681 tgagttaggc ttttattgct gctgttagat gtagtagttt gagtatcaga tgatgagctt
    58741 tcctgtttgt tattgtcatt tgcctcgttt gatgaggaaa gcgagaaacc tacggtagta
    58801 aaaataatcc cgataataag gagaatcata ggtgttttct tatttgtttt tttgataaat
    58861 cttacaatta acatgattaa acctataata aataaaacaa taccagtaaa accaaatata
    58921 ccgtccatta atagatcctc tttctctttt gatattgaat attataaacc aagcaattct
    58981 tttttcttaa aatcgaattc ttcttgatta ataatccctt catctaacaa ctctttatac
    59041 tttttaattt catctgcact tgaaatttgt gtttctttgg aattattatc taatatagat
    59101 tgttgcttct ctatatattt ttttaaattt tccatttcat tccaatattt ttttgtaaac
    59161 ataacggtgt tttcatcttt cgtggccgct aatatacctc cgcgactttc gtttccgcct
    59221 agcaaggtaa attgtatata accattactc atacctggtt tttttaattg tacggctgat
    59281 atatttttaa aggggatagt tttttctcct ttaatacctt gattaactaa atttataaat
    59341 ccttttctaa taattgaaat agaatcatca gttactttaa tcaaggtttt tcctggagat
    59401 tttattaaaa tctctttttc cattttttct tcctcatttc tgtgatatga ttttttttgt
    59461 agaagctcat atatgaggaa agagcccgtg ttgcagcacg ggctttttta atgttttgct
    59521 gaaatcggtt tttttaataa ttcttgatgt attcatacat attgccttgc gtaagaatat
    59581 tttttcttaa aatagcattg gcagacagca taacaagtgt atctgagctt attctgaata
    59641 gaataatact ccataaattt ttcgagatta aactgagatt tatcagtcag ttcgttctca
    59701 atatagatat ttaataaaat tagaatagct attttatctg cttctgtttc aaattttgag
    59761 tgaaaagttg tagaagtatc gtacaaaact gaaaattcaa aatgagaggc gctgaaatgt
    59821 gcaagttcat gagataaatg aaaggcttct gcagtttcac cgtatagatt ttcattcaaa
    59881 aaaatgattc tgggttttgg atagtagaaa ccaggctctt tcatctccat ataaactact
    59941 ttcaaattgt attcgctcaa catttctttc aatttcaaat acatacaaac catcactcca
    60001 actattcatt ttcctctaaa gctttagcaa ttgcaatcgc tttacgcatt gtctccttag
    60061 atatttcttt tccatcaaaa gaaaaaacag tatcgtcttc tgataaatcc acatgtttag
    60121 gggtttctct ttcttctcta cctagaaggt agtctacaga gacatcgaaa tagtcagcaa
    60181 tccgacttaa ttcatctgaa ttaggagtat tatttttcca ctttgctaga taaccgttag
    60241 aatatccaaa attaatttct aactgccgta tagatatctt tttcctcttc gccaagtctt
    60301 ttattatttc ataggtattc atcgataaaa caacctttct aaatgtttac aaaaaaagtt
    60361 tagaaaaata cgcagaaata atttgactaa ttctgagtaa tgagctatac tatatcttgt
    60421 aaacagattt aacaactaaa aagacaacaa aaaacactat tgatatgtaa atgcagaccg
    60481 ccaagaaagc tttaaaatca atgtttttat gtcttattta attatgctct tagtatagag
    60541 tattactcag atactgtcaa ttgaatttag aaaaaagttg ttaaatttgt ttacgaatat
    60601 aaaagaaagg aatgagaaat atgaacacac cgcaaatttt taatttcgaa caaaacgaag
    60661 ttcggacaat tttagtaaat gatgaaccat attttgtagg caaagacgtt gcgagtgttt
    60721 tgggttattc aaacactaaa gatgctttgt cgcggcatgt agatttggaa gataagatgg
    60781 ggtcgcgaat cacgacctca ggtcaatcaa gagagatgac aatcatcaac gaatctggtt
    60841 tgtacagttt aatcttaaaa tcaaaacttc cctctgccaa aaaatttaaa cgttgggtaa
    60901 caagtgaagt gttgccagca attagaaaac atggaggtta tctaactcca gaaaaagtag
    60961 aagaagcttt gcttaatcca gatacaatca ttcaattagc aactcaacta aaagaagaaa
    61021 gaactggaag attaatcgca gaacaaaaga ttgcagagta cgaacctaaa atctcctatt
    61081 tagatagcat attatcttct acagattcag taacaattag tcagattgca gcagattatg
    61141 ggatgtctcc acaacagatg aataaattac ttcataaact aggtattcag aaaaaagtcg
    61201 gtaaccaatg gttattgtgc aaaaaacaca tgaaccaagg atacacaaaa tctcatacaa
    61261 ctgagatccc gaaagccgat ggtggcacta aaattgtaat gaataccaaa tggacacaga
    61321 aagggcgtct atttatctac gaattactaa aaaaagaagg atattaccct caaatggatt
    61381 tagaggaaat tggttagaaa ggagttttag tatgactgac attgcagaaa tcactcaacg
    61441 agatagagaa aaaatcaaag aatatgtcga aagttcaaag ttcttaactt acaccatgct
    61501 tgctgaaaga tttggaatta gcaaaagcta cttatcttta attttaaacg gtaaaaagac
    61561 ttctgcagaa gcaaacagaa ttatagattc gattatcact atgtacgaat tgtaaaggga
    61621 ggaacagcta atgcaatatc tagaagcaaa aatcccaatt ccagaaggct atgtaattat
    61681 ctcccaagtg gattatgagg agttaaaaaa agctgatgat actggtagat ggatgacgtt
    61741 gccagaagtg ctagaacgga ttaacagaaa atatgattgg tttacttcta gagttttaaa
    61801 gaacccaaga tatagaaata ttatcgatat cgagaaaaac aaaaatggat ttgtctatta
    61861 tccagttgaa ggaagagaca catatctatt tttaagaagt aaaacacttg aatttttaga
    61921 aacaaatttt tcggaaatct taaggaggca agcggatggg aaaatttaat agagcgctag
    61981 tgttcagcgc accgctaatc atctacgctt taggactttg gggaagcagg caagcgttga
    62041 taggaacgat tgtttacatg gtttggattt ttatggggct tgatgaagct gaagctgagt
    62101 acagagcgaa aaagccaacc gagggggctg actaaaaatg aatagaaaag agaaactaga
    62161 atgtatatta ctattactca gtttaattct ttcactaatt tctctattgg gaagtttgta
    62221 tttttgatat caacaatttt gtggatttgg gttctattta gagtaaatat catttttgtt
    62281 tggtgattaa ggagtttatt aaaattaatt ttccctactt caaatgcaag gtaacaagat
    62341 atagcacttt taggaggaat atttagcggt acagcttcag agtatattct attatcatcg
    62401 actgatcctc ctttagcaat taatttctta aatggagtag ctgaccatct gcggtttaag
    62461 ttagcggatg aaagttctaa attaactaac atagctggtt ctgttgaata gttagaaatg
    62521 attacttttg tataaatcat atctgcagcg aagtaagaag cattaagttc aacttctagt
    62581 tgagggcgca ttccttttat ttttatccct gttaaaaacg tactcaatat aaaacttatt
    62641 attgacatcc attgaaaaat tgttaagtga aaaaaattca aaataaacac caccagtttt
    62701 taactaaatt ataccaaaaa ggagagaaga aataatgcaa gaattagtaa ttttgaaaaa
    62761 taaagaagct gtgactacga gcttacaagt ggcagaaagc ttcgagaaaa aacatcaaca
    62821 tgttttaaga gatattgatg cactaaaaaa agatgtgtcc aattttggac agatgtttgt
    62881 agaaggtaat gaaccagatt catatggcag aaatcgacga gttttcttca ttagtagaga
    62941 tggatttttc ttgctggcta tggggtttac aggaaagaaa gctatctact tcaaacaaaa
    63001 atacattgaa gcattcaacg aaatggaaga tgttattcgc aagaatactg ttcctcaaac
    63061 aattgaagat atgatgatct atcaattaga agaaatgaaa gatgttaaaa aagatgtttc
    63121 catgcttaaa gatactatgc gaattagcgg acaacaagag tttgaaatta agcaaaaagg
    63181 aaatatgaaa gttatgggag ttctaggggg aaaagaaagc cgagcttatg aagaaatcag
    63241 caaaaaagta ttctcaaaat tttggtctga atttaaacgt accttttcaa tcccaagata
    63301 tggcgagtta cctcgtaaga gattcgatga tgctgtttca tttattgaaa tgtggttgcc
    63361 agaaactgcg atccgtatgg aaattgatca actgaacaga caacagagac tttttggtga
    63421 tgacaatgaa tagagctgaa gcgctaagaa tagggacggc aattgctaat tgctggtgga
    63481 aatactataa accaatcatc ctaagccaac aacatattga caagcaaaaa gcatggcaac
    63541 aaataaaaaa gtgactccgc cggcaagcaa agagtcacaa agaaaacaca tcataaggag
    63601 attttagcat atggaaaaag aactttccac tctagatcaa tatttgattg atcctgattg
    63661 gggcaagccg aaaattgagg aaacaagtgg tcgaaaaatc agacgaaatc ttttgacgaa
    63721 tgaagaacta gcttgggatc aagatgattt aggcaaccat gtaactattt gggatcatgt
    63781 ttatcttatc catctatcga agcattcgaa taaacctgaa tatatttacg tcatcgaaga
    63841 tggcttgatt gatgcgctag aagagtacga cagagataac ttgattgata tctcttatta
    63901 cggaccaggt aagaaataca ttgctgaaat ggaggcagaa tttgatgagt gaaatcaaag
    63961 ggacaacgaa ctttgaaaaa ctttttagtc gtaagttaaa taaaattctc aagaaaaaag
    64021 gaaattttga ttatttatct tgggctcacg cgtgggagat tatgaaaaag aatgatccac
    64081 aggcaacggt aactattaat gagtacaaac actacagggt tgtttctgga actcatcaag
    64141 actttcttgt tgaggaatat aaaccttttc ttatggatga aactgggact tatgtatctg
    64201 tctcagtaac ggttaaagga cacacggaaa ccgaattatt tcctgtttta gattatcgaa
    64261 accaaccagt tgttaaacca aatgcaatgc aaatcaataa ctcattgaag cgatgctttg
    64321 tgaaagcatt ggctctacac ggactgggat tatatgtatt tcaaggggaa gatattccaa
    64381 caccacctag aatcgataca aagaaattaa acatgctaga gacgattcta gaagctttca
    64441 atgagcagat gggtaaagat atgaccaaaa ccttaatcga atatgttaat gagcagacag
    64501 ataaattagg gctcttagct gataacgttg aaactattga acagttaagc tatgagcaat
    64561 gtgccttgat ggagcgagca atagcagcta agagaaaaga attagataag aagtgatatg
    64621 agtgtttaaa ccattaatcg attcatattc agcggttctg aaaaagttca aaggaaaaga
    64681 cataagcgca accatcaatg aggaagtgaa cattgatcga ctaaagacga tgtatgacgg
    64741 ctacgatggt gatcgagtca ttgaaattcg ttttattgat cctagacgtt tcaccgtaca
    64801 gcaacgaaac ttcatctatg cgctgatagg cgatattttt atcgatacag gcatgccaac
    64861 ggacttctgg aaggaattct tctactttcg ttttgaaggt gtcacagggc gcaaaataag
    64921 cctgaaagac gaatcgaata caactgtgag tgatgccaat gtcttagcaa atatcatctt
    64981 agatttcatc tttgaacatc atattccttt caaagaaggc tatgagattt tacctgcgaa
    65041 tcaagaatat tacttctaca aatgcatcac aaaaagagtc tgctgcatct gtggcaaaac
    65101 aggagctgac atcgatcact ttgacaaagc gctaggaaga cgaaagcgca aagaagttga
    65161 tcatgcagag tacacatttg cagcactctg cagaatccat cacacagaga agcacaaaat
    65221 aggtgtgatt aatttcaaaa ataaatatca aatcaaaggg atcaaattaa accagaaaac
    65281 aatcaaaaag ttaaatattg gagggtaaaa atgacagaac atcgaagtta ttatgcgatt
    65341 ataccagcca acgtaaggta cgacaaaaga cttaaaccaa atactaagtt gttatacgga
    65401 gagataacgg ccttgtgtaa tgaaagaggc ttttgttggg caggcaatga gtactttgca
    65461 gatttatatg gtgtgaataa agagaccata tcgcgatggg taagtgattt gattaagttt
    65521 ggatacttga atcgggaaat catttacaaa gagggtacca atcaaataat caataggtac
    65581 ctacgaatta atcaataccc tattgacgaa aaacgcaata cccctattga cgaaaaagtc
    65641 aaagataata atacatctat taataataca tttaataata caaaagaata tataagagag
    65701 ttaccgcctt cgaaaaaatc gaaggctaag cccgtccgtc ataaatacgg agagtataaa
    65761 aatgttcttt tgtcagatga gcagatggag aaactcaaaa cagaattccc taatgattac
    65821 caagagcgaa tcgaacgact gtcagagtat tgtgaatcat ctggtaagac ttataaaaac
    65881 tatttggcaa ctattcgaag ttgggcaagg aaagaaaaaa gtgaacctaa gaacgcaagc
    65941 agtggataca agcgcacagg aagacgagag aagcttcctg aatgggcaat cgaccaagaa
    66001 gcctatctca agaaaaaagc gctagaacga gctaatagac aatcaaaagc accattctaa
    66061 gaggtggaaa aatgaagatc gattatctag aactaattaa tgaaatagcg aattataaaa
    66121 agggcgagga attagacgtc ctgagagacg tatatgatca actcgaagaa gctggaattg
    66181 aaggaattaa gaatgatcgt tcgagttgga gtaagctcag atactatttc gcactttata
    66241 tcgatacaac acaattaaga aatttagcat ataccaaatt actatttgtt gattgtgtta
    66301 aaggcttgca aaaacatctt aatgaacttg agcaggtgta atcagatgga cctaaagaca
    66361 tttacagcac agattgaact aatgcatcaa gaagctttaa gacaaagtgt atcgtacgaa
    66421 gacaagtggc tcaacacgtt ccatggcgga cgtgagagcg cacttgatca agtactcaaa
    66481 ttactgaaag gagaatgtcg ggatggataa gaaagcggca atgcagcgaa ttatcgaatt
    66541 gacttattca gaagattggc aaaatgacaa agaagctgct tcagaagtga tgaggcttgg
    66601 aagagcgatg tgggcagaca agagcaacaa gccaagacca cgaaaaatcg caatttggca
    66661 cggtgacaaa cttctagtga tagggacagc tgaacagtta gcaagtctca caggcttgca
    66721 cgagaaaatc gtgaggaaaa gagcaaggtg tggctacaca gacgctaaga agagaacgtt
    66781 tagatacgtg gaggaatcat catgacaaca gaagaagtga ttcaaatgcg tattcgaagc
    66841 cttcagcgtg agattgacga tctggaacga acaaaggcag tgatggtcaa tgaaacggcg
    66901 agaaaggcaa tcgatttgca catagagaat ttaagaaggg aaatccatcg attggaggaa
    66961 tgagcgtgga taagaaagca gcaatgaaaa gaattgctga attaaccaag tcagaatctt
    67021 ggcaagaaga caaagaaata gttgcagaag tccaaaagct cggtaaatca atgtggactg
    67081 aaaagcccaa acggagaacg ccgagaaaaa ttgcaatctg gcatgatgac cgaattttag
    67141 taacaggtac tgctgaacag ttatctgaaa ttactggatt aagcaaaaac attatctggg
    67201 atagagctag gagcttatgg attgattcaa aaggacgaca gtttaggtat gtggaggaga
    67261 gataatggat ctcattacac aatacagtga catcatcctc aagaaaatca tgatgaagat
    67321 tcagaaagat aaaaaatcaa aagaacgagc tgaattagtt aagttagaaa tggctgaaac
    67381 aggagcagga gtgcgaagta gcaggcattg gaaagcagca gcaaacattg aattttatta
    67441 caacgaaatt caaaaagggt tcgatcagat gcgtgagctg gatcggcaaa caaattggag
    67501 caagaaactt catcaagatc gtttcaaatt tgtagagaag tataaagaaa tattagaaga
    67561 gtatttgagg aggacagcaa atgataaaaa aactcgttca attcagcatg gatttatatg
    67621 atatcgaatc aggagcaaca ctatctgtgg aatcggacca tctaatcata aattttggtg
    67681 gaaagcgcca gattattttg tgggtagttg atgatgtact gtttccagaa attgttcatg
    67741 atttcgaaga atcaaaagcg gttgagtttg aaatagtgaa aaaagtaatg gaattgattg
    67801 aaaaatacga ggaggacagc gaatgatacc gaagtttaga gcgtgggata aacgaaagaa
    67861 cgtaatgaga gatgtagccg tcttgcattt tactaaaaac ggcaaaacaa actttattga
    67921 atattggata aatcctaccg aattgaaatc atatcacgtg cgaaacatcg acctcatgca
    67981 atccacagga atgaaagata agaatggtgt ggatatattt gaaggggata tagtattagt
    68041 cagcgtgcga aatggcttcg attacttaga taataaagtc tgtattgtca aaaattcaat
    68101 agattattcc ggattagttt gtgccactgt tgatgaagac ttagagtatc aaatttttaa
    68161 cacagagctg tttgaagaat acacgtacga agtcaccgga aatatatacg agaatagcga
    68221 gttattggag gaacagcgat gaataaacag gaagcaatca aaaagttaga aagtattaaa
    68281 gcgataggaa atgatgcaat agctgcttgc tataacgaga gtataaattc aggtattacg
    68341 ttaatgaaaa aaatagacga accgcagaaa ccagttattc cacaactcgt ggccggttgg
    68401 cttgagaaat ctacggaccc ttttacaaaa gctgaaaaaa tagcgtattt aatcaaatct
    68461 aaagatggtg attcatatta tttctgtgat tggtttgtac gagatggcat agtgacgcaa
    68521 gagcaaggag aagaattact cgcttgggca acaagacaat catatgaaac actattgagc
    68581 ctatacaacg gctacgaggt tgagaaagag ccgttatggg caataaagaa tgccgatgga
    68641 aactatctta ctaaatgtgc tttatgggga aaagatggag taaattatag ttttgaatgc
    68701 aatccatctc atcgattgct tttcactgat aaagcaacag cggatgctgc agcattgttg
    68761 gtgaatggaa cagtggaaga ggtggtagaa agatgaaact aaaagacgga ttttacgcta
    68821 gcagtcacgg tatcggcggt ttaatgctag atatgccgac aaagaaccct aaaacacgca
    68881 agaaatcaaa attcaaagtc ggtgacatgg tccgctgtga agcagaagag ttcgtttatc
    68941 cgttcagagg atacgttaaa aagatactgt caaactcagc aatcattcgt attgaaaata
    69001 caatgaagtg tgatcgttcg actgcaaaac acaagcatta tttagctgta gcgagattag
    69061 ttgacatgga agtaatcaag agcaaataaa aaaagccgga tcgctccgac tgattcaata
    69121 aatccaacac atttattata tcacataaag gagcggtttg acttgatgca attgttacga
    69181 gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt
    69241 ttggagcgga tggcaggtcg ctctttgata gatattaagt cgccgattat aacggatatg
    69301 ccgaaggcac cgaagcatgg caataaggca gaagacgcga ttattcagat gatggatata
    69361 gaaacggaga gagatgcgat tctagcggct ttgatggctc ttagtctgat tagtcgtcag
    69421 atactctact acagcttctg tgtgccagat agcttctcaa actacagaat tagccgtgaa
    69481 gtgggttatt cagaaagaag tatacaacgg atgaagtcgg aagctctaat agagtttgca
    69541 gaagcatata aacacggaaa aataattgct tataaataat ttggcggttt tttggcggaa
    69601 tgatggcggt ttttagacat ttaccagtgt tattatggta gtgtcgaaag attaggaaac
    69661 aggatcgaca aaataaacga agggaggcaa tctccctcat cgttttaaac ttcttcttag
    69721 atagacagca actaattatg aagaaaagga tgtgaattca actcctccta aattcttcat
    69781 cggttgctgt ttattttgat tagacagcag cactcacaaa actaaacatg ttcttgtcgt
    69841 ctttcaactg ctgctgtcta ttaatttatg tattggaggg aagaaaaatg aaattatcag
    69901 tagaaggcac tccagaagaa atacaagaat tgctccaagc tattggtagt agcaaggagc
    69961 aaaaggtaag tgtttctaat tcagatattg acggtatttg caattgttta aacagagaaa
    70021 aaatatctaa tccttactaa gagaaaatat gttcgttaat gaatttccaa ttttcctctg
    70081 tagctctacc ttgataattt ttttgcagtt cacagatgaa taaggaatcg ttattatcaa
    70141 gtactgtatt taatttttca cacatttgat ttggattcaa attagtttta actaaccaaa
    70201 aagatttttg tagctttata tatgccccaa attcttttat tatttcaaat actttatcat
    70261 acttttgccc tgggttatta agatcataac taatcatgta gggtttatac atgttgtctt
    70321 gcacatcctt gttttatatt tcagcggacc actcgctgat aaataaaatt atacgcttag
    70381 tatttatttt cacaatatta atttatcgta ataacttttg agtaaataga acaaaaaacc
    70441 tgcactagtt tccgctagta caggcagtga ctatatcggt tgataatcta gcatatatta
    70501 aaaataattg caagaaaaga aatttatagt ggtttatacc aaattatcaa agcaatgtta
    70561 ttttgttgct gtctattgtt ttttaattat tcacacgata actaaaggtg ggtgaagaga
    70621 aatgattcca ttaataattt caatttttgc gctctgtctt aatgtctata tgattggatt
    70681 taaaaatggg caaaataaaa aatagtagca gccaagaata attttatagt gtcactgtgg
    70741 cggaaagggt agacgctaag catgtgtgct aggtcaatgc ttcggcaacc atgcaaggtt
    70801 cgattccttg ccagcgacat taaatgccta tgacggttac gactaccgaa aaaagatcgt
    70861 taagaagcta tacggtgcta cgtacggcaa tgtagtaagt gtgctatctg tacaccacca
    70921 agcttcggtc actgtggcgg aagtagaaga cgcagcggta aatggcgagt agcctcgtga
    70981 gagcctggta agttctcgtg agtggtgcaa tcccactcca gcgactttaa gcaaccgagg
    71041 catcggcggt ttaaaaatat aggggtgcgc aatttcgtac gcgttttgtg catcgtgcaa
    71101 gttactatta catattagat cactctttga gtggtctttt tatttttgca caaaggagga
    71161 aacaacaatg tatagaccac aatacttaga acagaagtat gaagtaatca ctgttcataa
    71221 aggtaatggc gaaaaagtat atgagtatag aagaccaata aagagcgata catataaacg
    71281 aaaggaaaac aatgaagtta ttccattgta tggcaaaaga atagctaagc attaaataag
    71341 attgcgaaag gagacggaac atgaccgagg aattctatag atggctatta cagttgacaa
    71401 gagaagatcg tttggttaag ttctatcagt ctcctaaatg gcgcaggctt agagagaaag
    71461 cgatgaaacg agatcactat gaatgccaag agtgtagaag actaggtaag tatcatagag
    71521 tagagaacgt tcatcatata aaggaagtca aggatagacc tgacttagct ttagatttag
    71581 ataatcttat ttgtttatgt gttgaacatc ataatgaagt tcatggcaga tatcttacag
    71641 cattagataa acaagagaag aagatagaaa gctttgctaa cttcgatgca agtgaaaggt
    71701 ggtaagtgca tgatcatcaa tgataatggc agagagtatg atacagaaaa gattgaagag
    71761 tattcatctt atactcaggg attaattaaa cgtttgatat acgttcgcta tgtaggtatt
    71821 agggatctgt tatcagataa ctgttgtagt aaatacaaag tgaatcaagt aagagaagcg
    71881 ttgaataaag ataataacgt cgaaagaata aaaaatgttt ttggatatag tattgaagag
    71941 attaattatt acattgactt cgctgaagct ttcattccga tggtgagata accccccctt
    72001 aaaataaatc gcaaattttt tgggggtgat gaaacggagg gggctgtcag gaaaagagat
    72061 tttttcgaac tttatcatga aaggagggct aaaatgttta aaaacgaatt gtctcaaaat
    72121 cgctacagag aaaaattacg ccgctcttta ataagccaat tggaaagtca gaaaacaaat
    72181 attgagccat tcttagataa tgttgatcgt tatatcagtt tatgggaaac ggcgatatca
    72241 ctggaagaag atatatccga gaacggcatt agactggaga atggtaaaaa gaatgaatca
    72301 gtagcgttgc ttgtttctgt caacaaacaa atgggattga tgttggataa acttgccatt
    72361 actcctgaat tggtaggtga agcaaatgaa tcaattcctg agttataagc atattgaaaa
    72421 ttggttcaaa gctatagaag aaggcactat caaggtatgc aaagagcaat tattgctaaa
    72481 aaattatcta gaagaaagag tctttactag agaagatatt tacttcgata agcagatggt
    72541 agaggattca atcaatatac cagcacaata ctttccattc gaattaattc cgtgggaaaa
    72601 atttctacaa tgttttattt atggtgttcg atggaaaaaa gataaaacac tagtgttcaa
    72661 tagatatctt tcattaatgg gacgtggtaa tggtaaaact ggttttgctt cttggaacaa
    72721 cttctttcta ctaaccgcta aacacggtat taaaaattat gatattgata tctatgccaa
    72781 taatgaaagc caagcaaaga ctagttttga tgatgtattt aaagtaatta aagatcatcc
    72841 tgatttagat aaaaaagtat ttaaagctac gaaggaagtt attcaaaata tcgctacaaa
    72901 cagcaaactt cgttataaca cggcaaatgc tagaacaaaa gatgggaagc gaccaggtgc
    72961 aaaccgcttt gatgaaattc acgaaaatga agattattca atgataaatg tggctacttc
    73021 tggtggtggt aaaattcgag attatagaga attttatgat acaactaatg gtcatgttcg
    73081 tggtggtccg cttgatgaca ttatagaaga atcaaaaatg attctttctg gagaacttgg
    73141 aattgacaag gatggagcag aattttctag tttgtttcca tttatttgtc gcttggataa
    73201 cgataatgaa gttgatgatc ccgacatgtg ggaaaaagct tgtccaacta ttaattacaa
    73261 tgcagattta aaacggaaaa tgtttcaaga atactctcaa atgcaacgta atgctggttt
    73321 aagacttacg ttcatgacca aacgaatgaa cagacctatg gaagatacac gatttgctgt
    73381 tgcttcatat gatgatgttc tgcatacgaa agaaaaagaa tttcctgaaa aaatggatga
    73441 agtgatagga acagtcgatt ttgctgatag acgagatttt gccagcgttg ggttgctagg
    73501 aaaatacgat aaagatgttt attttacaca acatactttt atccacgaat cagcccttcg
    73561 attacaaaac atcaaacgag aggttataga tatttctata gatcaaggaa aatcacagat
    73621 cgttcatgga aaaaatatag aagctgatta tattgtaggt tggtttcttg aaatgagtaa
    73681 taaatattat attaaaaaaa tcgctatgga tatgtaccgt gcaaaaatat tgaagcccgc
    73741 tttagaagaa gcaggtttta ctgtggaaat tgttcgaagc ggatctgtta cacatggtat
    73801 gttaaaagat ctggttgatg acctttttat taatcaacgt ttattttttg gtgacgatgc
    73861 gattatgcgt tggtattgca tgaatgtata tgaagagcat atttctaatg gaaatatacg
    73921 ctatgaaaaa atagaacctg aaactagaaa aacggatggc tttttttcat tccttcatgg
    73981 tttgaatttt ttagatgata tttatgattc tgctcctgta acagtcacaa atagctcagt
    74041 agaaaataca ggaactggat ttactcctct agtattctaa cttgaaagga ggtgagaaag
    74101 tggggatttt tcaaaaggcg gtaggatact tcacaaaaaa agcaacggtt cctttagaag
    74161 aatacttttg taaattgcaa gttgattttg tgtatcgaaa atttgcgatt gaaacttgta
    74221 ttgatttgat tgcaaatgcg atgagtaaag cggaattcaa gtcatatgaa gatggaaaaa
    74281 ataaaaagaa tgatctttac tataggctga atgtagctcc taataagaaa aataatgcaa
    74341 cagaatttag aaaaaaactg atcaggagat taatattcta caatgaagta ttgatcgttt
    74401 ctccgtctaa taattctagc gaaatattta ttgcggatag ttgggatgtc acagaatatg
    74461 cattgaaaga tgatgtgttt tctcaagtgc aaattaacaa catagtcctt gatagagaat
    74521 ttctagaaag tgatgttatc tatataaaat acgcagatca acaaattagg caactagtcg
    74581 atgcgtatta tcaagcgtat gggaaactca tttctagtgc catgaatgtt tacaagcgct
    74641 ctaacgctcg tagatacgta ctgaaaggga atttattccg accgcaagac aatacaacac
    74701 aagatcaaat caataaaatg atgacatcac aatttaaggc ttttatggaa gctgataatg
    74761 caggtgcggt atttcaatta caaaatgagt acacattaga agatttcagc ggaaacttcc
    74821 aaagcaattc aagagatata aaaaacttaa tagacgacat ctttgagatg acagcagcag
    74881 cgtttcacgt tccgaaaaac ctactaaagg gagacatgag tgggttatcg gatcaagtgg
    74941 acgctttttt aatgttcgaa atcataccga ttgctgaact tattcaggat gcgtttaacg
    75001 ctagtctcta tgaagtagaa gaatacttgt cagggaattt tgtacgtgtg gatacaacta
    75061 tgatcaagat tactagcttc aaagatttgg ttgacgctat tgatgtaggc attagaaatg
    75121 gagtatttac aatcaacgaa ggaagagaac gcgttggaaa tgatcgctct gataaggcga
    75181 tggcagatga aatatttata actaaaaaca atcaacaagt atcgaaagga ggtgaggcga
    75241 atgacgacaa tgaaaacatt tctagcagta aagaatgaag gcgcagtacc gcaaattttt
    75301 attcagggat ttattggttc tagttggttc tttgaaggga atactgacaa gggaatcaaa
    75361 aatattttgg atagtctagg tgatcaagaa gaaattgaag tagtaattaa ttcaaacggt
    75421 ggagacgtat ttcaagggat tgctattggg aacttactta agtcaaataa agcaaaagtt
    75481 aacgttgtga ttaacggctt agccgctagt gctgcttcaa ttatcgcaat ggctggcgat
    75541 actataaaaa tctacaacaa tgcacaattg atgattcacc gcgcttccac atacggagaa
    75601 ggaaatgtcg atgacttccg tacgattgct gaccaactgg aatcaattga taaatcggta
    75661 aaggcttcat ataaaacacg attcaatggc acagatgaag cattgcaaga acttcttgaa
    75721 aaagaatcgt ttatggatgc agaaacagct ttgagttatg gattggtcga tgaaattatc
    75781 gatgcagaaa atagctcagg tactgaagct aaaaaagaac aaagcgttga agaaattttg
    75841 aatgacgtta aagaaaaaag agcagaaaaa attgctgcat ttacagcagc attaaataaa
    75901 acatttggac aaggagatgt aaaataatga cagttaaaaa tttaaaaggt gtaacagctg
    75961 caagcgacca attgatgaaa gcttttaaag atggtaacga agaatctttt agcgcagcta
    76021 tggtaagctt atctaaggaa attcaggata aaattttaga agaagcaaca gcaaaaaatc
    76081 aagatcaatt agtattaatg aaccgtggtc agcgtgtatt aactacacaa gaaacaaaat
    76141 tctataacga agtagtgaaa aacgaaggtt ttgcaggggt cgaagaatta gtgccagcta
    76201 ctgtatttga acgtgtattt gaagatttag aacaatctca tccactattg caaaaaatta
    76261 cttttgttaa cacaactggt gtaacagaat ggattgtgtc acgtggagtc aatccagcat
    76321 ggtggggtaa actttgcgaa gctgttaaaa aagttttaga taatggcttt gatgtaatta
    76381 acatgaagca gttcaagcta tcaggttata ttcctgtatg taaggcaatg cttgatttag
    76441 gtccagtatg gttagatcgt tatgtccgta ctgttttagt agaatcattg agaattgcat
    76501 tagaacaagc aattgttgat ggtactggta aagatatgcc agtcggaatg atgcgtgaca
    76561 tgagcaaaca aactagcgga gaatatgctg aaaaaaaagc agaacctatt acagctttag
    76621 atgctgtaac tatgggcggt ttgatggcgc gactatcaaa attcaatatc gaaggtgtga
    76681 atgatccgat ttatcgtaat gtgaatcctt ctgatgtggt cctaattgtg aatccaactg
    76741 attactggtc taaagtattc ccagctaaga ctgtactaac tgctaatgga gaatacgtac
    76801 aagtattgcc agtaccagtt tcagatttgc agtcaacggc tgtgccagaa ggaaaagcag
    76861 ttattggggt agcctcagat tacttcatgg gtgtaggatc tacactaaaa attgaagctt
    76921 cagatgaata ccattttgtt gaagacgaac gcatttatct agctaaacaa tatgcaaacg
    76981 gtcaacctaa acgtaacgat agtttcattg tattagatat tagcgctttg ggaactacta
    77041 ctacaactac aaaaccaaca accacaacaa ctacaacaca agcgtaggtg atcagaatga
    77101 agtatattct ttgtcagccg gcaatcaatc ggtttaaatg ggagcttgaa gtttgtttaa
    77161 ctaatctgaa gaaactagga atcaaagata tcgtattgct tttcagcaga cacgatgatc
    77221 agattcctat tttttttgag aaggaatatg gtgttgaagt tcatgtgtac gatgatctgc
    77281 gggacgacaa agagtatatt ccttcgatta aaccatattt atggtggaaa tatttagaag
    77341 aagatcattc gcgtgaggac gaccgatatt tctatatcga ttcggatgtc attttcaata
    77401 aaagaattaa tttgcgcaaa ttgccttcta aagatgatgt ttggtattgt agcgactgct
    77461 gtagttatct aagtcttgat tatattagaa gctgtgaaaa cggagaaaat attctaaaag
    77521 atatggcaaa cattgtaaat gttacagtag aatctttgga aactataaac actaattcag
    77581 gaggcgcaca gtgggttatt aaccgtccta aagcgaatta ttggaaaaag gtttatctgg
    77641 attctaatcg gctatatcgc taccttagag ggcaaaaaac aaatatacaa atctggacag
    77701 ccgagatgtg ggcacagctt tggaacatga tgtatttcaa tattggtcct aaagttcacg
    77761 aggaattaga cttttgtttt gctactgatc caatagaaaa agttaaagaa gtaaaaatct
    77821 tacacaatgc tggagtaaca acaaacgatg aagatttatt tttcaaaggg agatacgtga
    77881 cttctacgcc ttttgatgaa gatttatcat ttgtaaacaa gaaaaaatgc tcttacgcat
    77941 atgttaaagc aattaaggcg gtggttagat gacgcctgaa caagtgactg aagaattgct
    78001 aatagctgtg aaggataata tttacgttac ctggaacgaa gaagatgagt caattaaaaa
    78061 gatgatagct aaaaatgctg tttatcttca aagtaaagtg agtacaacac tttctttttc
    78121 tcctgaaagc ttagaatacg gattgctaat cgaaagatgt agatacgact ggaatcgtgc
    78181 tttagatgag tttgaacaaa atttcgctag tgagttatta ggtttcattc aacattatgc
    78241 gctacaagaa tatattgcag gtgatgtgaa tggcgaataa tcgtagactc gaagaaacat
    78301 tcaacgatgg ttggttaaag attttgacgc aaactaccaa aagaaatgaa ctaggaaaaa
    78361 agattggtgt agaagataca gaaatcactt ctttaaaatt tagaaatctt tccatgagag
    78421 atagtgatat aacagctatg gatgcgatgg gatcgaaatt aactaagaaa gtaaagactc
    78481 catttcatcc aatcgccaag aaatttaata aagatcaata ttttatcgta atcgatagta
    78541 tgcgttacaa cgttatctat gccgattacg ataattttta tatctatttt tatcttgaaa
    78601 gtgtgggtga atatggtgat tgataattct aaagaaaaag aacgtttaaa taagcaaatt
    78661 tctgctatca aaacttcctt agaagagcat tttggcctca aactctttca agactccgta
    78721 ggcgaggatg agctacctga tgattttaat tacttcattc tcgaaacagg agaaatagaa
    78781 atgatcactg agccaaaata tagcgtgggt caaaatctat atctaacttt ctattcagaa
    78841 aatagagaag atttaacagg agactcacta gatattattt cattgattca aaatcgttcg
    78901 attcgttttc agagaatgga tcccaatcat ttaaaactag agaaccaaga tcgctatatc
    78961 gatcaattgg tatttacgtt tagacgatta ttgaagagtg attgtcatgg ctaaaaatag
    79021 ttgggagcta aaaataaatg gacatgatga acttcttgtg cggatggaac gctattcaag
    79081 cgagagcgaa cgactgatta acgaagcatt gaaatcaaaa ggttcggcta ttgcagtgga
    79141 taggattaca gaaaaaattc ctgtttctga agcagattta agaagagggc accaacacgc
    79201 aaaaaatagt cgtccactta agactcaata cattaatttg ggtttcatca ttagacctac
    79261 aagaaaattt gagtatttaa aatatcctga tttggggata ggtacttcta aaagaaatca
    79321 gccggacgaa tttatgagaa gaggattagg tcttgcactt gatccaatta cagaacttct
    79381 gattcgtcaa ttcgataaat taaataaata gggggaacaa caatggctaa aacaaaaact
    79441 gtagtaacaa cgttcgataa cgtaagtatc aaacgaattg cttttaattt taagaacgca
    79501 ggaaatgcaa tcgcaacgaa ttgtaacgga caattagatg gcgaaacaga aatgcaaacg
    79561 gtggttaaaa aatgtggagc gacagaagta aaatcaaaat ctaaaccaat caatatgacg
    79621 gtaacaatta ctgcacatgt accgatggaa gtttatcgac gtttcaatgg gttgaaacaa
    79681 gatggacgta ttaaaccagg catttactct tacggtcctg attccgtagg cgaagatttc
    79741 tcacttgctg cagagatcgt ggatgacttc gaagaaaata gaaagttagt tggtatgtta
    79801 gcatgcactt cgaatacagg attaacattc tctattgaaa atggtgcgga tgaagtagct
    79861 gcgttagaac tagaaacaaa agttatgcaa gatgaatttg gtaaatttta tcatgaagca
    79921 attgttgcag aacttgaaga agacttaaca gatcaatgga tgacgaatct atctgctgat
    79981 gtgattaaaa aaacttcagt tgtgacaact acggccactc aatcacagta aaaaaaacgg
    80041 aggtagcgaa atgaacgaag attactcaaa aattgaacta aacgatggaa caattttgaa
    80101 tttagaacct aaactgaata tcaagaaatt attgatgatc aatagagatt ttaacacaga
    80161 cgagtttgca aaaatgactg tgggaaaagg atccatggat atttctgtta ttcaaggtgc
    80221 aaaggctgtg tatattgctt accgccaagc gaacatgact gattatattt cattcgatga
    80281 atttatcgat aaatgggatt ttgatatggc tactgccagc tatatttatc aattgatgat
    80341 gttcaaacaa gcacgcgatg cttatcaaaa agaatttgaa aaagcaaata aggaaaaaaa
    80401 gcttcaaaag taaaaattcc aaagctctta gttgaaacgt gggtcgatgt ctattcgatg
    80461 ttgaccgacg ttttttctat gccttcagat ttggttttaa gcgatatctg tttagatgac
    80521 attttgcaaa tggcttacaa caagagtgct tatgaaggat ggaaaaacta tgcaataaac
    80581 caatcccaga aaaactaaag aaaggaggta aaaaatggct aaaaagagaa cagaagcaga
    80641 agtaactttc atagctaacg atgacggatt gaaatctacg ttaaaagaaa tcagtgctga
    80701 attaactaaa aatagagcag aattaaaact agaacaagct caattacaac agactggttc
    80761 tgaatcagac aagttaggaa gtaaattatc ttctttagaa aagcagtatg aattacaaag
    80821 tcaaaaagtt gaagtaacta gccaacgttt agccaatgcg aaaaaatatt atggagaaaa
    80881 ttccaccgaa gttaagaaac ttgaaagaga actgattaat caacaaacag cgcaacaacg
    80941 tttgtcaaac gaaattgata aaacgagtaa tgcactagct caagcaaaag gcgaaataca
    81001 gacgtacgag tctacaatgc aacagttgga tagcgaacaa aaaaatgttc aagctagtgc
    81061 ttctctgatt gaatccgaat acaaaaaatg gcaagcaact gctggtcaat cagcttctga
    81121 agccgagaaa ttagcgaaag cccaagaata tgtttctcaa caatctgaaa atgcggagaa
    81181 aacgatagat atcctaagac gacagttaga agctacacag tctgaatttg gcgctacatc
    81241 cacagaagca atgcagatgg aagcgaagct taatgatgct gaacgtgaat ttgaagagtt
    81301 aggacaagct gctaaaaatg tagatacaac taacttggac gatatcggaa gcaaaataga
    81361 tatgaataat ttaatggaag cttctgacgt tttaagcgac attggcgata agcttacaga
    81421 attagggaaa caagcagtgg actctgctaa cagtgtaggt agttcccaga gtaagataca
    81481 agctaatttt ggtttgtcta aacaagaggc tgaagaatta acgaatgtag ccagagacat
    81541 ttattataaa ggttttggag aatcgttaga tcagtccaca gatgcattga ttttggtaaa
    81601 gcgtaattta ggagatttaa ataatcaaga tttacaaaat atcacggaac aagctatggt
    81661 cctagaaaac accatgggcg ctgatatgga tgaaacgtta cgtggtgtaa atggcttaat
    81721 ggtcaatttc ggcttgagtg ctcaagatgc aatggattta atggtttcgg gtactcaaaa
    81781 cggtttagat aaaacgcacg aattaggcga caatatggca gaatatagcc aattatggag
    81841 tcaaatggga tattcagctg atgaaacgtt cggaatgctt caaaatggtt tagatgcggg
    81901 tgcttataac cttgataaag tcaatgactt agttaaggaa atgggaatat cgttaacaga
    81961 tggtcgattt gagcaaaaca tggatatgtt tagtgaaagt actagaaaag cttttgaaga
    82021 gtggaaaaat ggcggaggaa cacaaaaaga cgttattaat tccatgattc aagattttag
    82081 caatatggat ggtcaatacg accaattaaa taaagcttcg acaatttggt ctgcgcttgg
    82141 cgaagataat gcgatgaaag ttgtccaatc tttaactgat gttaaccata catttgatga
    82201 tgttagtgga tctgcacaaa aaatgaatga agattctact actccgttgc aagagttgaa
    82261 cgggaaaata gctgaattaa aggattcatt agctcctata ggcaacacaa tcatagatgc
    82321 actcgaacca gtaattgatt ttctaggaaa gatggctgat gcgtttaata atcttccaca
    82381 accagtacag gatttcattg ttgctatagg tggtcttaca gcagcatttg gcatactgtt
    82441 accggcaata ctagccgtgt catatttatt tggtccgatg atgcttataa ttggaggaat
    82501 tatagctgtt atagcaggcg ttattgtggc aattaagaac tggggtgcaa ttactgactg
    82561 gtttagtggt ttatggaaaa aatttactga ttggttgggt gatacttggg aaagtataaa
    82621 agacggagcc tcatcagttt gggatggggt taaagaaacc tggtctggat ttgtagattg
    82681 ggttcaagat atttggcaag gagtttctga ttggtttgga gagctatgga gcggattagt
    82741 tgaaggagct tccaacatct ggcaaggagt ccaagaaact tggcaaacat tcgttgattg
    82801 ggtttcaaat atttggaacg gagtcaaaga agtatggtcg attatttggg cagacattgt
    82861 aggaattgtt caaataccat ggacattaat aacgtcattg attcaagctg gtattaatat
    82921 tatcgtgggt atttttgatg tagctggaca gttattgggc gcagcttggc aagctgtttg
    82981 gacacctatt tctgatttcc ttaaaaatac ttgggatact atgacacaat ggataagtat
    83041 cgcttggaat gggattgtaa ctacattcca tactatattt gatccagtag tggcatggtg
    83101 gaatggtata tggacagcta ttagtactac ggcttcaaat atttggaatt caattagtgc
    83161 aacagcttct agtatttgga acagtatcaa gaatacaatc actagcttgg tacaagcagc
    83221 tgctacagta attcaaaata tttggtcaac tgtatctagt tggttaggtg gaatttggaa
    83281 ttcaatcagc tctacagcat caaatatctg gaatagtgtg actagtagta taagcaatgc
    83341 tataaacgca gctaaaagtg ccattcaaag tgtttggaat agtatatctt cgtggattag
    83401 cggaatttgg aacggtatca aaaatactgc tttgaatctt tggaatggaa ttacaagcac
    83461 tattagctct aaagtaaacg atggaaaaaa tgcaatttca agcggttggt ccaatctaac
    83521 aggtattgtt tccgacatat tcaataatgt taaaagtaca attgctaaca tatgggaagg
    83581 catcaaaaag actgttagcg ctccaattga ttggattaga gacaaaatca gtggcatttt
    83641 tgataatttg aatatttcta taccacatat tccgttaccg gaatttatca tggaaggcag
    83701 ctttaacccg ctaaaaggtc aaatccccca tttgcgtgta aaatggcatg ctaaaggagg
    83761 tatctttact aaaccaactt tactaggtgg aatgaacggt gtcggtgaag caggacctga
    83821 agcagtttta cctttgaaaa gatctgtttt gcaagaaatt ggtgatcgta tcttgagtag
    83881 cacatcagtt tcatctaggg cacaaacgat tcaacctgtg aataactacg aattcaattt
    83941 cacaattgat ggtaacgcag atgaggttac tatgaagcaa acaactcaac aaatcattga
    84001 tagcattaca aaaattcaaa atgataatgc ttcggcatgg cgttaaacag gagagtattt
    84061 ctcctgtttt tttagtatta aaaaggatgt gaaaaaatga ctgattgtat acattctata
    84121 atcgatggat ttcctgatta tttgcataaa ttggctttag ctgaaagacc aaccatacct
    84181 tctccaaaaa gacagagagt tgaaacttct gttttaggta ggttaggtgg cttagtacaa
    84241 gattactcgt ttgaagatat gtcgtttaca ttgcactata actatttaga ggatgtggaa
    84301 gaccatcaag cgttcaagca atcgttttat atcatgcgtc attggttaaa ctatgcaaag
    84361 aaattagaat tctctgacga tcccaacgtc tattacgtta tccagactat cgatattggg
    84421 gatgcagaaa acgatattgt tgaatgggga gagttcgatg taaatattac tgcgaaacca
    84481 ttcgcaagag ttcaagaaga tgtacctata accgtagata aaccacagtc atttaacttg
    84541 ctgaataata gtttagaaga aagttttcca aagattatca tcactccttc agctacttca
    84601 tgccagttca tcttaaatga ttatgtgttt agtttcgaag gcttagtagc aggaactgat
    84661 gtagtcattg atagtgattt gatgctttgc tacgaagagc aatcggacgg agatatttta
    84721 gatcggtcca acaaaatgaa gaccatgcaa tatccgacat tgcaagtgga tatcaatcat
    84781 tttaattgca ctggtttgag caaaatacaa atttatcgta acgggttaag gtaggtgaaa
    84841 tagatgatcg ataatttaat aactatttac gataaaaatg acgcgaataa tttagctgaa
    84901 catttatatg atacgcaagg tttaggcgct ttgtcagact ggttaacagc tactgttagc
    84961 aataaactaa acggagccga gatatttcag ggtacttatt caataaacgg aactaatgca
    85021 gatttgattg tagaaggacg tattattcag tgttatgtag atgaaaatcg agcaaaacag
    85081 cgtctacgga tttattatgc aaaaacttct gtaataggca atacgataga agtaaaagct
    85141 gaacctattt tcaatgatat aagaaaatcg gtgttgaata aatatgacag cggaacagaa
    85201 aagatcactg ctactcaggc atggcaaaac gcaaaagttt tagcgaaacc agctatccct
    85261 tcgcagtttt ctttctcgtc attagtagat acgcttgcta atgtgaagat agaaaaggcg
    85321 aattttttag aattctttgg tggaaaagag ggatctattc tagatcgatt tcatggtgag
    85381 tttctaaaag ataataacac attacgtcat gaaaaaagtc taggtacgga tcataaaatc
    85441 aaagcgattt atactaaaaa cttaactggt cttgacttag agattgatgc tcaaagtgtt
    85501 ttagttggag tttatccatt cattagcagc tcttcagaag gagaagacga gatcactcta
    85561 ccagaagaag ttattttcac ggattacgtg gatgattatc ctgctggata tgtttctttt
    85621 gttgatttta aagacaaagc gactgatgta gccacattaa gggaagctgc taaagactgg
    85681 ttgaaaacaa acatagataa acaaaaacca caagtgagtg gttcgattga attagtacca
    85741 ttgaggcatc aaagaggcta tgaaaaattt gttgatctag aaaaagtttc gatgggtgac
    85801 ggagtagatg tgtatcatcc acagttaaaa gtgaatatgt cagcgagaat tgtggaatat
    85861 acgtttaatg ttctaaccaa ttcatacgat aaattagttg taggaaacgt caaaacaaac
    85921 ttcttagaaa acacagagaa taatgtcagc aatttgatta atgatgccat tgatcaattg
    85981 aaaaacggtg gcgaaatcag tgatttactc aatgatattg tagatcatca aactgatatg
    86041 attactggtc aaaatggtgg ttatgtttta ttagatccta aagaagcgcc tagtcgtatt
    86101 ttgattatgg acacaccaga taagaatacc gcaaggaatg ttttacaaat caacaatgct
    86161 ggtattggtt tttctaaaac tggcattaat ggaacatatg aaacggcatg gacgttagat
    86221 ggcggattca atgcctcgtt tattacggct ggtgaaatag taggaattac tattagaggt
    86281 actacattaa ttagtgatgg tgctgattat agaacaagta ttgctaatgg caaaatgact
    86341 tggtactcaa aaaaagttaa caaagatatt atggagctag aagcacgtga ttatgtaagt
    86401 gctgatgccg gtattgtatc atacaccatg aaaactggtg gtggtttcat gattagaaat
    86461 ccacagggta acttggtttt tagtacgtgg gataatggta ataacagacc gtttctatct
    86521 tttggtgcgc ccaatttcag gtatagcaat gctagttatg taacttctgg cgacggtagt
    86581 tctttaagca ttaatggtag tgcgggtaac tcatgggaat ttaaggtagc tggtaggact
    86641 atgaaattta ctagtgatgg tatgctaacg ttaccaggtt gtttttttgg ttcatgggaa
    86701 gatgggaaac ttgctaggtt tgaacaatca acggtacaag tatataaaga ttttactgtt
    86761 agaggtacta aaaactcaac tgtaccaaca gaacattatg gacaacgact attgaacgct
    86821 tatgaaactc cagaatatta tttcgctgat tatggggaag ccgttacagg tgacaatggt
    86881 aaagttcgtg ttgatattga ccccatgttt gctgaaacag taaatctaag tcggtatatg
    86941 acacatgtga cacctacaga actagttttg tgtgctgtta ctcatgaaga tattgaccat
    87001 ttcatcattg aaactagtaa gccaaacgta ttagttagat ggaatttagt ggcacaccgt
    87061 ctagggtatg aagatattag attaaaagag gatacagcat atgatagcac agtgcttgac
    87121 caaaaacgtt tttaaaacga agacaaggag gtatataaat ggctagcagt ttatataatt
    87181 tggctttaga tttcagcaaa gaattaaact acaccaaagc tattatggct cgtcaaggtg
    87241 ataaagggat tacggtgacg gttaaaccgt ttctaaatgg cttgcagatg gatacgagtg
    87301 gcggaacatt tactttaaaa ggaacaacac catctaaccg ttacgtagat aatgttgcaa
    87361 ctagtgtaac tagtgaagaa gtcacgtttt ctcttgatgg cacatttatg agtgaagcag
    87421 gatattataa acactgctac gtagaatata gaaaagacaa tcaaatttta acaacgcaag
    87481 atatcatttt tttctcacta ggagtgtctg acatttcgca aggccaagcc gatgaatatg
    87541 tttcgcaatt agaagagttg attcgaaagt acaacgaaac ttttgatgct tttatggctg
    87601 aaatcaaagg tagagtggat agcttaaatc aacagattac tgatttaact ggtcaagcta
    87661 aaacgctaca agacaagtta gatgctctga aagaagaaat ttctaagtta ggtaacttac
    87721 aagtgatgta cagtaacagc atcgacttcg gggactatga ttattcggag aacccgaatt
    87781 taatgccata tattacagaa ccttgggttg gacctttgtt aggtaatgga catacagtta
    87841 aagatagtgt taaaagggtt ataactcata ctaaaactag aacagctaat tccggcgaca
    87901 tattatcgct tggcttaggt attccgtgta cagcggaagc taataatagg tatcttataa
    87961 ccactttacg accatctacg acatacacat taagcgttac tatgagtgtc ggttcagatt
    88021 ggactggaga gaccaatact ataggggtta ggcttagata tttaaatgaa caaggtggta
    88081 tagaactgcc cattaatgca ttaataccag caaatgtaga acgcgataaa atggtcactc
    88141 atacatttac tggtataaca aaagataatg taactagcat aacaaactgt tatgttgaaa
    88201 tattctcact taatagtgaa tataaaggaa cggttagtgt cagctatgac gttaagttaa
    88261 aagcacacta ccctaactta ctagatggcc cttactggtt aggtaaagtt cctttaggcg
    88321 agaatattgc tgaccctaca gttgtctttc cacacaaaac tagtgaatat atggtttatg
    88381 gtagacgcaa tacagaaaac tatatcgctg accaaacata taccatatca atgaaagcaa
    88441 ctaaactaac agttcaatcg tttgccgtgt atattgctgc tggccgcgtt aaagtaggag
    88501 acatgaagcc tactgagggg ttagctaata catgggagtt aacttttact gtaactaagc
    88561 aacatataga tagtggggta actaattatc tggaaatata ccaatacccg tcagctacaa
    88621 aaggagctgt tcaaattgag tggctcaaac tagaaaaagg caatactcga accccgaata
    88681 ttagtgagta taaatatcgt ggtactggta tgcgtgattc aaacaatcca aaagattatg
    88741 tttgggatct agcaccagaa tatgtcgaag ataatttggc cacagatatt aaaatttctg
    88801 aaattactgg taaagcaaac aattataccg atgggaaagt atcggagatt aattcgcagt
    88861 tgactgcttc aattaatgaa gtagacacca cagctaagga tgctcaaaca aaagcgaatg
    88921 ctaatgcgac tgctatagat gaattagaca ataagatcga tgaacgcatt aatgatacag
    88981 ctactaccac attaacagtt acaaacggga ataccggatc agcaaagctt tatcgtgaag
    89041 gaaaaacagt ttctatatat tttgtggctt taaacggaaa aagcagtggt ggaaatgatt
    89101 caacgatact aacaattcca gaaggctatc ggccaccaat tagttttgag caactggttg
    89161 gctcgataga ccgttctact ttgaacagtg ctcagttatc tattggtgca gatggagcca
    89221 ttaaatggcg aagaaactca agttatggat cggattatac ctttgcaatt acttacacga
    89281 tttagaaaag cgtgaatcga tatgaaggca gcatatagac caattgaacc ttacggattc
    89341 gagcaaatca ttgtgaatga tgaagaacat ttaccggaag aatgcacaga agtcgaacca
    89401 ccgattccaa attggaaacc gaaattcaat tactgggagg gaaataaatg aaaaacattt
    89461 ggaaatatgg acgtactggc ggagagtacg caggaaaagt attggacgac atgcttgtat
    89521 ccgttcctta cacagatcag ccaccgcttg aaggaattcg tgctgatggc gaaccgttaa
    89581 caatcgctga tcaaatgttt gatcctaaat tgaatcaatg gattatttta gcgaacgcac
    89641 tagatcacaa cgatttaaac aatctcaaag caatgtatga gtcgttagaa aatgagaacg
    89701 gcgatttaaa acagatcaat gccaaactca tgctaagcga tgtagcaatt aaacaggaaa
    89761 atactgcatt gaaagaaaaa gcggatagtt tagcacaaat caattcaaaa atgatgcttg
    89821 cttcgttaca aaatagcaaa gacatttcag aaattaaaga gcaactaaat ccagcttcaa
    89881 agggaggtga gtagtatgtt tagttttagc gatgtgaaaa tgatgtatga ttggggctgt
    89941 ttcactaacg aacaagtaat ggttttcgtt ccgttgtgca ttactgaaga aaaagcagat
    90001 aaaatcatta gcaaagaaga gagcgcatct taattgatgt gctttttatt ttgattcaag
    90061 gagttgtcac atgattaatt taggggaatg gggagcgata gcaggatcaa taaccgctat
    90121 cgtttctttg attttattag taataaaacc aattactgca tctttctcga agattactga
    90181 gactctttca aaagtaagtc acaatttaga tttgctgact aaagatttag aatcgagcaa
    90241 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
    90301 tgcagaaaaa ttggtagaac acacgcaaca aattaaaact ttatttaggg agagaagaaa
    90361 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
    90421 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
    90481 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
    90541 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
    90601 ttactgcgat gagcctgtta acggctcttt ttttattacc cattaatacg tttgcttata
    90661 ctattaatga cgagtataat ttagcgccga atcaaggaga ctccagatta gcaattccta
    90721 acaaaattat tttgcacgaa actggaatag atgcaccagc aagaaacgta gccgccaaca
    90781 tgaaaaataa ttataacgga agcaatcctt atactacaga tgttattggt gacggtggga
    90841 ttgtttaccg tgtgggtgag caaggatatg tttcgtgggg agctggtaac gctaatcctt
    90901 atgcgcctgt acagattgaa ttacagcgca catatgataa agcattgttt gaaaaaaact
    90961 atcgagctta tattgaatat acaagagata gtgcaaaaaa atatggaatt ccattgactc
    91021 ttgatcaagg aacttcttta tttacaaaag gaatcatttc tcatttgtgg gtgacaaatt
    91081 atgtttgggg gaaccacaca gatccatatg gttacttatc gcaaatggga gttagcaaag
    91141 aaaagcttgc ttatgattta gctcatggat ttaccgatga aaatccaaca acttctgaaa
    91201 acaagcctgt cattgatcca acacgagctg gtgcagctaa tcctacgctg acagatggaa
    91261 caaatcacgc ccacattgat cagtttgggg aaatcgaaaa cgcaaacttg catgtggctg
    91321 gatggcacat tgctaactat aaatacgagt atattttcat tatggactac aatactggga
    91381 aagaattagc tcgagtaaga gctgatggaa tttatagacc agatgtaaac caagcttata
    91441 atacttctgg aaatgttggt tatcatgtat ctttcaatat gcgtaatttt cctagtaaga
    91501 aagtctatgt aatgatgcgg gcaacgaatg atccagaggg aaacactaaa ggcggtgcgc
    91561 aagatttcca tgacaaacgt tggtatttaa atattcctaa acgataaaaa tagctcctcg
    91621 ttgaggagca gtacataact atattgacaa ctataaaaat tattcgataa aatagtgatg
    91681 ttatcgcata tcttcactat cacccat
//
LOCUS       DACEUF010000003        70988 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.003, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000003 DACEUF010000000
VERSION     DACEUF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 70988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.003"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            336..1439
                     /locus_tag="IXG20_000220"
     CDS             336..1439
                     /locus_tag="IXG20_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1608440.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1723..2403
                     /locus_tag="IXG20_000221"
     CDS             1723..2403
                     /locus_tag="IXG20_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1608441.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2493..3527
                     /locus_tag="IXG20_000222"
     CDS             2493..3527
                     /locus_tag="IXG20_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1608442.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3541..3876
                     /locus_tag="IXG20_000223"
     CDS             3541..3876
                     /locus_tag="IXG20_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608443.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3860..6340
                     /locus_tag="IXG20_000224"
     CDS             3860..6340
                     /locus_tag="IXG20_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608444.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6396..7955
                     /locus_tag="IXG20_000225"
     CDS             6396..7955
                     /locus_tag="IXG20_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1608445.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7952..9316
                     /locus_tag="IXG20_000226"
     CDS             7952..9316
                     /locus_tag="IXG20_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1608446.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9487..10557
                     /locus_tag="IXG20_000227"
     CDS             9487..10557
                     /locus_tag="IXG20_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1608447.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10572..11240
                     /locus_tag="IXG20_000228"
     CDS             10572..11240
                     /locus_tag="IXG20_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1608448.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11260..11838
                     /locus_tag="IXG20_000229"
     CDS             11260..11838
                     /locus_tag="IXG20_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1608449.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12241..13152
                     /locus_tag="IXG20_000230"
     CDS             12241..13152
                     /locus_tag="IXG20_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1608450.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13408..14325)
                     /locus_tag="IXG20_000231"
     CDS             complement(13408..14325)
                     /locus_tag="IXG20_000231"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ1608451.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14527..14964
                     /gene="zapA"
                     /locus_tag="IXG20_000232"
     CDS             14527..14964
                     /gene="zapA"
                     /locus_tag="IXG20_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ1608452.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15022..15570
                     /locus_tag="IXG20_000233"
     CDS             15022..15570
                     /locus_tag="IXG20_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ1608453.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15685..18045
                     /locus_tag="IXG20_000234"
     CDS             15685..18045
                     /locus_tag="IXG20_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1608454.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18132..18446
                     /gene="trxA"
                     /locus_tag="IXG20_000235"
     CDS             18132..18446
                     /gene="trxA"
                     /locus_tag="IXG20_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ1608455.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18628..20406
                     /gene="uvrC"
                     /locus_tag="IXG20_000236"
     CDS             18628..20406
                     /gene="uvrC"
                     /locus_tag="IXG20_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ1608456.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20551..21450
                     /locus_tag="IXG20_000237"
     CDS             20551..21450
                     /locus_tag="IXG20_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1608457.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21443..22705
                     /locus_tag="IXG20_000238"
     CDS             21443..22705
                     /locus_tag="IXG20_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1608458.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22868..24766
                     /locus_tag="IXG20_000239"
     CDS             22868..24766
                     /locus_tag="IXG20_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ1608459.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25065..25784)
                     /locus_tag="IXG20_000240"
     CDS             complement(25065..25784)
                     /locus_tag="IXG20_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ1608460.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26121..26453
                     /locus_tag="IXG20_000241"
     CDS             26121..26453
                     /locus_tag="IXG20_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608461.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26440..28443
                     /locus_tag="IXG20_000242"
     CDS             26440..28443
                     /locus_tag="IXG20_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ1608462.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKHQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28462..28932
                     /locus_tag="IXG20_000243"
     CDS             28462..28932
                     /locus_tag="IXG20_000243"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ1608463.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28953..29537
                     /locus_tag="IXG20_000244"
     CDS             28953..29537
                     /locus_tag="IXG20_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ1608464.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29552..30538
                     /locus_tag="IXG20_000245"
     CDS             29552..30538
                     /locus_tag="IXG20_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ1608465.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30528..30839
                     /locus_tag="IXG20_000246"
     CDS             30528..30839
                     /locus_tag="IXG20_000246"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ1608466.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30861..32642
                     /locus_tag="IXG20_000247"
     CDS             30861..32642
                     /locus_tag="IXG20_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ1608467.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32635..34011
                     /locus_tag="IXG20_000248"
     CDS             32635..34011
                     /locus_tag="IXG20_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ1608468.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34018..34653
                     /locus_tag="IXG20_000249"
     CDS             34018..34653
                     /locus_tag="IXG20_000249"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ1608469.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34683..36038
                     /locus_tag="IXG20_000250"
     CDS             34683..36038
                     /locus_tag="IXG20_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ1608470.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36230..37624)
                     /locus_tag="IXG20_000251"
     CDS             complement(36230..37624)
                     /locus_tag="IXG20_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1608471.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFNSPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37716..38687
                     /locus_tag="IXG20_000252"
     CDS             37716..38687
                     /locus_tag="IXG20_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ1608472.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38684..39466
                     /locus_tag="IXG20_000253"
     CDS             38684..39466
                     /locus_tag="IXG20_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ1608473.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39635..40006)
                     /locus_tag="IXG20_000254"
     CDS             complement(39635..40006)
                     /locus_tag="IXG20_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1608474.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            40140..40583
                     /locus_tag="IXG20_000255"
     CDS             40140..40583
                     /locus_tag="IXG20_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1608475.1"
                     /translation="MDKPISGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40626..41435)
                     /gene="yidA"
                     /locus_tag="IXG20_000256"
     CDS             complement(40626..41435)
                     /gene="yidA"
                     /locus_tag="IXG20_000256"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ1608476.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41454..42821)
                     /locus_tag="IXG20_000257"
     CDS             complement(41454..42821)
                     /locus_tag="IXG20_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1608477.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42805..43635)
                     /locus_tag="IXG20_000258"
     CDS             complement(42805..43635)
                     /locus_tag="IXG20_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ1608478.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43798..44235
                     /locus_tag="IXG20_000259"
     CDS             43798..44235
                     /locus_tag="IXG20_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ1608479.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            44311..44931
                     /locus_tag="IXG20_000260"
     CDS             44311..44931
                     /locus_tag="IXG20_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ1608480.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            45120..45626
                     /locus_tag="IXG20_000261"
     CDS             45120..45626
                     /locus_tag="IXG20_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ1608481.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45643..46542
                     /locus_tag="IXG20_000262"
     CDS             45643..46542
                     /locus_tag="IXG20_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ1608482.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46629..47513
                     /locus_tag="IXG20_000263"
     CDS             46629..47513
                     /locus_tag="IXG20_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1608483.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(47705..47776)
                     /locus_tag="IXG20_000264"
     tRNA            complement(47705..47776)
                     /locus_tag="IXG20_000264"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47741..47743),aa:Arg,seq:ccg)
     gene            47836..49170
                     /gene="rpoN"
                     /locus_tag="IXG20_000265"
     CDS             47836..49170
                     /gene="rpoN"
                     /locus_tag="IXG20_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ1608484.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            49373..50410
                     /locus_tag="IXG20_000266"
     CDS             49373..50410
                     /locus_tag="IXG20_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ1608485.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            50451..51452
                     /gene="gap"
                     /locus_tag="IXG20_000267"
     CDS             50451..51452
                     /gene="gap"
                     /locus_tag="IXG20_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1608486.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            51562..52752
                     /locus_tag="IXG20_000268"
     CDS             51562..52752
                     /locus_tag="IXG20_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ1608487.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            52856..53611
                     /locus_tag="IXG20_000269"
     CDS             52856..53611
                     /locus_tag="IXG20_000269"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ1608488.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            53727..55025
                     /gene="eno"
                     /locus_tag="IXG20_000270"
     CDS             53727..55025
                     /gene="eno"
                     /locus_tag="IXG20_000270"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ1608489.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            55425..56564
                     /locus_tag="IXG20_000271"
     CDS             55425..56564
                     /locus_tag="IXG20_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ1608490.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(56631..57206)
                     /locus_tag="IXG20_000272"
     CDS             complement(56631..57206)
                     /locus_tag="IXG20_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1608491.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(57211..58185)
                     /locus_tag="IXG20_000273"
     CDS             complement(57211..58185)
                     /locus_tag="IXG20_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ1608492.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(58209..58253)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            58468..59436
                     /locus_tag="IXG20_000274"
     CDS             58468..59436
                     /locus_tag="IXG20_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1608493.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            59549..59827
                     /locus_tag="IXG20_000275"
     CDS             59549..59827
                     /locus_tag="IXG20_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1608494.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            59945..60181
                     /gene="secG"
                     /locus_tag="IXG20_000276"
     CDS             59945..60181
                     /gene="secG"
                     /locus_tag="IXG20_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ1608495.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            60293..61039
                     /locus_tag="IXG20_000277"
     CDS             60293..61039
                     /locus_tag="IXG20_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1608496.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            61042..63399
                     /gene="rnr"
                     /locus_tag="IXG20_000278"
     CDS             61042..63399
                     /gene="rnr"
                     /locus_tag="IXG20_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ1608497.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            63421..63885
                     /gene="smpB"
                     /locus_tag="IXG20_000279"
     CDS             63421..63885
                     /gene="smpB"
                     /locus_tag="IXG20_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ1608498.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            64187..64906
                     /gene="atpB"
                     /locus_tag="IXG20_000280"
     CDS             64187..64906
                     /gene="atpB"
                     /locus_tag="IXG20_000280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ1608499.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            64959..65174
                     /gene="atpE"
                     /locus_tag="IXG20_000281"
     CDS             64959..65174
                     /gene="atpE"
                     /locus_tag="IXG20_000281"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ1608500.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            65265..65789
                     /gene="atpF"
                     /locus_tag="IXG20_000282"
     CDS             65265..65789
                     /gene="atpF"
                     /locus_tag="IXG20_000282"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ1608501.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            65776..66318
                     /locus_tag="IXG20_000283"
     CDS             65776..66318
                     /locus_tag="IXG20_000283"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ1608502.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            66341..67897
                     /locus_tag="IXG20_000284"
     CDS             66341..67897
                     /locus_tag="IXG20_000284"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ1608503.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            67911..68813
                     /locus_tag="IXG20_000285"
     CDS             67911..68813
                     /locus_tag="IXG20_000285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ1608504.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            68836..70242
                     /gene="atpD"
                     /locus_tag="IXG20_000286"
     CDS             68836..70242
                     /gene="atpD"
                     /locus_tag="IXG20_000286"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ1608505.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            70256..70678
                     /locus_tag="IXG20_000287"
     CDS             70256..70678
                     /locus_tag="IXG20_000287"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ1608506.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT        23555 a        12436 c        15089 g        19908 t
ORIGIN      
        1 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
       61 gctttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
      121 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
      181 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
      241 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
      301 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
      361 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
      421 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
      481 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
      541 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
      601 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
      661 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
      721 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
      781 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
      841 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
      901 tcccgctgcc aatgccaatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
      961 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     1021 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
     1081 tagaatctgg tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg
     1141 ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     1201 tcgtgtttga cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg
     1261 ccgatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     1321 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     1381 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     1441 aaataaatga cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt
     1501 tcaaggatgt cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca
     1561 tacttaataa aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat
     1621 acaaaaaggg tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt
     1681 ctctactata cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt
     1741 gggacattat tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag
     1801 ggtcaagcga cttcaaaagg agatattacc tttactgaac caacaaatac cgttgagcct
     1861 cttaacccta ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact
     1921 ggccagacag gttctttaac attggatgta gttcctgaat tgccatttgg tacacacgag
     1981 attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc
     2041 caagtatcag atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca
     2101 gtttcggttt cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt
     2161 ggaacatcca ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact
     2221 gtaactgggt tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa
     2281 ggtctgggaa catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa
     2341 gcagcagctg gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca
     2401 tagagtatga aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta
     2461 ccgcagttgt tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt
     2521 cgtttttttc tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt
     2581 cagcaggaga ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg
     2641 gttattttga tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca
     2701 attcttcaga tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg
     2761 gtggaacgat cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg
     2821 tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa
     2881 ctatacctat agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta
     2941 ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca
     3001 aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac
     3061 cggatctgaa acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc
     3121 ttcgtttcca aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct
     3181 tttatgaaaa tcagctttat attgaaaata cgtctaatga atttttagtt gcaccaaatt
     3241 cgaattttca cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg
     3301 cagaaatcat agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga
     3361 tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat
     3421 ctttcccatg gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc
     3481 tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg
     3541 ttgaaaacat cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa
     3601 gagccagtcc aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca
     3661 gttccgcatc caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt
     3721 tataaagaat ctaatcaaca tggcgtattc ccggagacga atgaagaaaa gatccgtttt
     3781 ctttatttga tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga
     3841 aaggaaaagg aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt
     3901 ttttacttcg ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg
     3961 agcaacgcct cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa
     4021 tggtggacgt agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt
     4081 gcctaaattg actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac
     4141 ggtccgtttt cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat
     4201 cactgcacaa ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag
     4261 tgatgtatta aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat
     4321 caaggttact agcggtagtt tattatcagc aggaagccat accttgcgtt ttgggatggg
     4381 gtcgtgggat acaaatactc tattggcaga tattattgct attattacgc gaggcgaatt
     4441 caacgtggac gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga
     4501 aaacggagat aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc
     4561 ttctatgtct aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca
     4621 agatcgtatg ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac
     4681 tggcggaaac ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg
     4741 gaatagttac atctcaacaa gtgattcaac aggaaaatat aatacatttg ctgataccac
     4801 agaatacgtg gatggaaata accagaacct tccaggctct accaatagtc ggacattgac
     4861 gatggggctg actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt
     4921 caactatttt acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag
     4981 cacaagtgga ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg
     5041 attgctttct cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt
     5101 accaacaaac ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca
     5161 agtcacggcc aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta
     5221 ttcgaaagga acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca
     5281 atcaaataac gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca
     5341 atattatttt gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc
     5401 gtacaaatgg cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa
     5461 ttttccatct gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg
     5521 agatcccctg tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac
     5581 aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac
     5641 ttatatcaat ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca
     5701 aggagtttct gtcccagcag atctattgag caaaatagga gataccattc atcttactta
     5761 caactatcaa ttaaatactg tagatacttc tgtacaatcc gtcagcatac tgacaaaggc
     5821 agctgtactt tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt
     5881 ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc
     5941 agatgatttt acatttggta acgatctccc caaaccgttg aagacaagtt attatgaagc
     6001 aaaaggcgat ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca
     6061 actgacaggg acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac
     6121 aaaattatac ttcaatcatt ctggatcaaa acaattgatt cagcaaggtc aaaacacgtt
     6181 gatttatgag agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga
     6241 tggtttactc ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg
     6301 catggtcaca tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga
     6361 tctgtgataa ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa
     6421 aatcagaaat gcttggagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac
     6481 aacggcaaat caaacttttc caacttttat tagaggaaaa aggagagatt tccgttaata
     6541 acgtacttac ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac
     6601 aagctaactt ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat
     6661 taatcagaca tggaaatata gcagcagtcg atgtttatta tcattgctac ttgcgctatt
     6721 ctacaaaata tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac
     6781 ttgtcatgga acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg
     6841 attctttaaa tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc
     6901 aaatttgtta tttttatttc caattatttt ggtttggacg tccttatctt gaaaacaaaa
     6961 aagaatacag tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt
     7021 ttactattac agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc
     7081 gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc
     7141 aaacgacaca atttttattg gacctccata catttttgac ccgttacttc tttcaatatg
     7201 cctttccttg gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta
     7261 cattagatac acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg
     7321 acgtagtagc tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt
     7381 atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc
     7441 attttgccta ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt
     7501 taactgaagt agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat
     7561 ttgctattgt tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc
     7621 gctatgcatc tattttgcgg acgctgatca agcagttaga attacgtctg caaattgctt
     7681 atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat
     7741 tgctgaatac gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca
     7801 ttttgactag ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca
     7861 ctagcaacga atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt
     7921 tagaaaaatt gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca
     7981 tgcaggcatc acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact
     8041 gatcgatgaa gatcataaag atggactagg ctatgtttca aatggaatca acctttattt
     8101 aaaaggaaag atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag
     8161 tggggcaaaa ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct
     8221 catcctcgca tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc
     8281 aacaggttcc tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta
     8341 taaaaatcta gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt
     8401 gatcttcggt gctggttata ttggattgga attagcagat gctttgagaa ataaaggata
     8461 cattattcat ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat
     8521 gatcaacagt tttcaaaatc agctgcaaac taagcaaatc aatttttatc caaatgaatt
     8581 cttgatcgat tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tatctcaagc
     8641 aataaaggca gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa
     8701 agaaaaagtg gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag
     8761 tgatccggac atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg
     8821 gcatctcttt ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac
     8881 tttgagtggt cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat
     8941 agactacttt ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa
     9001 taccggttcg tgttcaggag aatttgatct atttccacaa tacgatgaag aaaacaaaac
     9061 agtaaatgca aagctgatct accatccgga tacattagaa ataattggcg gacagctgat
     9121 aagccaagaa ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac
     9181 tacgattcca cagctagcag ttgaggattt tggatttctg tctgaacaca cctctcgttt
     9241 tcactacctc aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt
     9301 agataaaaga ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta
     9361 atcaatcgag aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata
     9421 ttcgccaaaa atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg
     9481 agttaaatgt ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt
     9541 gcaggggtga tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc
     9601 ttagctcaag ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg
     9661 aaagatgcaa attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc
     9721 gaaggtggga aagtggcgta tcttgcccag acacctggag tcgtgactag taaagacagt
     9781 acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg
     9841 cccaatcttg ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt
     9901 ttgaaagaag aatatggtct agccgtagga gatacagtta agttatctgg tagtgacaag
     9961 acgtttaaac taactggctt tacggatcat gccaaattca atgtctcacc agttctctat
    10021 acaaccatca atgcttacca agagatccgc ttcgaaaaag aagatacaag tgaaaatgca
    10081 cggattaacg caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag
    10141 atcaaaattt ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt
    10201 ggatttatga tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat
    10261 gtactgacga tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga
    10321 ggatttattg cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt
    10381 cttggtctat taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa
    10441 agcaattggc tgttttttgg cgtcattagc ttgttgatgc tggtagtagc tgtgttaggc
    10501 gcactatttt ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa
    10561 ggaggaagag catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg
    10621 accaagtagt taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg
    10681 caatcatcgg tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc
    10741 agacgcctag cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa
    10801 gtgaacgcgt taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg
    10861 ttccttttct gacagtaaaa aagcagttat tattagtaga taagatcaaa catgaacatc
    10921 gtcaaaaaga agcaaaagaa ttatttgagc agcttggcgt ggataagttg atcaacaagt
    10981 atccagaaga actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca
    11041 atgatccgtc cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac
    11101 gagaagtagt cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg
    11161 ttactcacga tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg
    11221 tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa
    11281 gatacacagc aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc
    11341 ggcacttcaa caagagagat cgcacaaaaa gttgggatca cccaacccaa tctctatcat
    11401 tatttcggtg ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt
    11461 ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca
    11521 aaaatggcgc agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat
    11581 cttgaaagca atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat
    11641 tatcgtgaac cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt
    11701 tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg
    11761 aaaagacctg tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt
    11821 gtattaggag aagcgtaaag ctgttatata gggagaaaaa tagttaggtg aagaagcaag
    11881 atatgacgaa gatcgttata tcttgttttt tttatatagg tagtttttca taaatacaaa
    11941 tttgttattc tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga
    12001 agtgttttaa tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt
    12061 gggtagatga atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa
    12121 aaatcatttt agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc
    12181 gtacttaagc tgattttaag ttttgaccct tagtctggat ctacgaggtg agagtcaaga
    12241 atgaaaaaaa taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa
    12301 atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg
    12361 gttacaaaga caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga
    12421 aaaaatgacc gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac
    12481 ggcggagagc tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat
    12541 gatggctata ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa
    12601 acaaaaccgg aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat
    12661 gatactgggg cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca
    12721 gccataaaag taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact
    12781 acagatgaat tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat
    12841 gtctttatca gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg
    12901 acatattatt actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact
    12961 gatctgcctc tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat
    13021 cttcaacctt ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa
    13081 ttagtaacag acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact
    13141 tattttcagt aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg
    13201 ccgggcttat tatcgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgagcc
    13261 tgccgtttaa ctccgcgtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga
    13321 ctattgtgac ttgtgaccga ggagttggca tgagaacacc gtttattcgg aacaagggac
    13381 tgtgacatga cttttgtcac agttccatta tttttttagc agcttcaaag ctttttctgt
    13441 attggcaaaa tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag
    13501 ttttgccgct acttggtcag atttcgttcc agctcctgat ggaagggtga ttccagcttc
    13561 agctgatgct ttgtgtaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc
    13621 aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt
    13681 tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat
    13741 cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa
    13801 agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga
    13861 taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg
    13921 gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc
    13981 acgtttcaca taagcagcac atactgttag cggaccaaaa tagctgccat ttccgacctc
    14041 atcactacct aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc
    14101 tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt
    14161 accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc
    14221 agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat
    14281 ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt
    14341 tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca
    14401 tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa
    14461 cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg
    14521 attcgtatgg cgcacgaaaa aacacgctac aaagcagtga ttgccaacca aacatatact
    14581 attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag
    14641 ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg
    14701 gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatcttgga gctggaagaa
    14761 gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa
    14821 cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat
    14881 cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa
    14941 attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt
    15001 tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg
    15061 tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta
    15121 ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac
    15181 attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca ggtatttgct
    15241 tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc
    15301 tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg
    15361 agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata
    15421 tttttgattt tgcagttact tgcctttgtt ccattagatg tggtccaaaa tcaattcggc
    15481 gagcatagtt tcgctcgttt tatcgttgag agaacgccat ttcttaccaa caagatgtat
    15541 gagctttggg taacgaatat tttgaattaa ggaaaatagt gctggaaaat atttcgtttt
    15601 ttgtacagag tagtttattt aagaaggctg cgtagcgtat gtccggcaca gctctttttt
    15661 catagtgaaa gataaggtga taaaatgaat aaccgaatcc ttgaaacatt ggaatttgaa
    15721 aaagtcaaac agatggtcag acaatttgta gtgactgcac aaggaaaaga agaactggca
    15781 gaattggtac cagtgagtga gaaacaaacg atcacaaact ggcttcaaga aactgaggat
    15841 ggattgaaag tgcaacgtct gcgaggcggg atccctatac ctaaacttga aaatatccgt
    15901 ccccatatga aacggatcga gatcggagca gacttgaatg gagtggagct tgcacaagtt
    15961 tccagagtat tgtctacgac aagcgaattg aatcgtttca ttgatgatct ttctgacagt
    16021 gaaatcgaat ttgcacgttt gtatatgtgg gcagatcagt tagtcacgat ccctgttttg
    16081 agccgccgtt taaaggaagc aatcgatgaa gacggacgcg tgactgatga cgcttctcct
    16141 gagttaaaga gcataagaca gaacattcgg cgaagtgaac aagctgttcg tgaacagtta
    16201 gatgggatcg tccgtgggaa aaatgcaaag tatttaagtg atgcgatcat caccatgcga
    16261 aatgaccgct acgttatccc agtcaaacaa gaataccgcg gtgtatttgg cggtgtagtc
    16321 cacgatcaaa gtgcttcagg acagacttta tttattgaac caaaacaagt ggtggatttg
    16381 aataatcgtt tgcgtcaata ccaaatcgct gaacgaaatg agattcaacg tattttaagt
    16441 gaactttctg ctgaacttgt tccccatcgt caagaaatca tccacaatgc ttatgtgatt
    16501 ggtaaaatgg atctgatgaa tgcaaaagca cgatttggta aagaagtaaa agccatcgtg
    16561 ccgggaatca gtgaagacaa tcatgtcgtc ttgaaacaag caagacatcc actgatcgat
    16621 caggaaaaag tagtttctaa tgacatcaca atcggaaaag actatcaagc catcgtcatt
    16681 acaggaccta acacaggtgg gaaaacgatc acgttgaaga ctttaggtct gttacaactg
    16741 atggggcaag caggattacc gattcctgca ggtgaagaaa gtcaaatcgg tatttttgag
    16801 gaagtatttg cggatatagg agacgaacag tcgattgagc aaagtctatc gaccttttct
    16861 tctcatatga cgaacactgt agatatttta tcaaaagtca atgaaaaaag tctggtatta
    16921 tttgacgagc tcggtgcagg aacagatccg caagaaggtg ctgctctagc aatcgcaatc
    16981 ttagatgatc ttggtaaaaa gtctgcttat gtgatggcaa caacgcatta tcctgaattg
    17041 aaagtttatg gatacaatcg ggcaaatacg attaatgcca gcatggagtt cgatgtggat
    17101 acactaagtc caacgtatcg tttgctgatt ggcgttcctg gacgaagcaa tgcgtttgaa
    17161 atctctagcc gattaggtct tgatacagaa gttattgatg aagcgaagca gttgatgaat
    17221 gatgagagcc aagatttaaa cgaaatgatc acagatttag agaatcgccg gaaaatggca
    17281 gagaccgaat atctggaaat gcgtcatttt gtttctgagg cacaagaact tcacgatgat
    17341 ttgaaagaag cttacagcta tttctttgaa gaacgtgaaa aagaaatgga aaaagcaaag
    17401 aaaaaagcaa acgaagtcgt gtcagaggcg gaagaaaaag cagaaaaaat cattgctgat
    17461 attcgtaaaa tgcaacagca gattgggcaa ggaaatgtga aagagcatca gttgatcgat
    17521 gcaaagaccc aattagcaaa tctacatcaa gaagaaactt tgaagaaaaa taaagtattg
    17581 aagaaggcga aagaacaaaa aacgttgaaa ccgggagacg aagtgttggt tacgacttac
    17641 ggccaaagag ggacgctttt acgtaaaaac gggaatcagt ggcaagtaga aatcggtata
    17701 ttaaaaatga atgtgtccga agacgagctg acgcctgttg ctcctcaaaa agagccgaca
    17761 caacgagtta tccatgctgt acgatctgaa tctagtagtc acgtgccgaa tcaattggac
    17821 cttcgaggaa aacgatacga agaagcgcta agtgaggtag atcaatattt ggattctgcc
    17881 attctagctg gttatcctca agtgactatt gtccacggaa aaggaacagg cgcacttcgc
    17941 aagggaatta ctgattatct taaaaaccat cggagcgtca aaagctttga gtttgcaccg
    18001 gcaaaccaag gcggaaatgg tgcgacaatt gttaaattta agtaaagctt actaaaggta
    18061 agcagatggt tcgctatcca agtatagaaa tgctatacta atcatagatt ttaaaaatgg
    18121 aggtctttga tatgtcacaa gtaattacag ataagacatt taatgaagaa acagataaag
    18181 gacttgtatt gattgatttt tgggcaactt ggtgtggtcc atgtcgtatg caagcaccga
    18241 ttttggatca gttggaacaa gaatatgatg aagaagaatt cagaattgct aaaatggatg
    18301 tggatgaaaa tccagaaact cctcaacaat tcggtatcat gagcattcca acattaatgt
    18361 tgaaaaaaga tggtcaagta gtagaaaaag ctgttggtgt tcattcaaaa gaacaactac
    18421 gtcaaatgat cgatcaatat ttataaaaac ttacagcaaa aataagctga gggtgggact
    18481 gaatccagtt ccgtcctcgg tttttttgcg gttgagttaa ggttattcaa attaatattt
    18541 tttgagtata attacgttgt caggctggat tgtttttttg acagacatgc tataataact
    18601 ttaattgaat atgaagggga gtaccagatg aacgaacgaa tcaaaaacaa attagcttta
    18661 cttcctgatc agcccggctg ttacttgatg aaagataaaa atggaacgat catttatgtc
    18721 ggcaaagcca aaatattaaa aaatcgtgtc cgctcatatt ttactggcag ccataatacg
    18781 aaaacggaac ggcttgttag tgaaatcgaa gactttgaat acatcgttac agaatcgaat
    18841 atcgaagcat tgctcttaga gatcaatctg ataaagaaaa atgatcctaa atacaacatt
    18901 atgttaaagg atgataaaac ctatcctttc cttaagataa cgaatgaaaa atatccccgt
    18961 ttggtgatta ctcgtaaagt actaaaagac aaagcactgt attttggacc atatcccgat
    19021 gtcggtgcgg cgaatgagac aaagaagatc ctcgatcgat tatttcctct gagaaaatgc
    19081 aaacctagtc agacgaagga gccttgtctt tactatcatt tgggacaatg tctgtgtcct
    19141 tattatttcg acgtagatcc gaaagtctat acgaaaatcg ttgatgaagt gaaaaagttt
    19201 ttaaacggag gacacgaaaa aatcgagtca gagatccatc agaaaatgga aaaagctgct
    19261 gaaaacatgg agtttgaaaa agctgcagaa taccgagacc agatccgtgc cattgagaca
    19321 atcatgactc gccaaaaaat gacaaatact gatttactgg atcgagatgt atttggttat
    19381 gcgattgaca aaggatggat gtgtgtccaa gtgtttttcg taagacaagg aaagctaatt
    19441 gaacgagatg tttcaatgtt tcctttctat aacgaagcag aagaagattt cttgacttac
    19501 ctcggtcagt tttatcagga aaatgagcat ttcattccta aagaagtctt gattcctgat
    19561 acgatcgaca agcccagtgt agaagctttg ttagctacga aagtgctgca accaaaacga
    19621 ggagaaaaga aaaaattagt tgaattggcg aataagaatg cctcagttgc tttgaatgaa
    19681 cggtttgatc tgattgccag aaaacaggaa cggacgatcg gagcagttga acgtttagga
    19741 aacgcgatga atatcccgac cccaatacgt atcgagtctt ttgataactc taatatcatg
    19801 gggacgaatc ctgtggctgc gatggtcgta ttcattgatg gaaaaccggc aagaaaagaa
    19861 tatcggaaat ataaaataaa aacagtcaac ggaccggatg attatgcatc aatgagagaa
    19921 gtcatctatc gcagatattc acgagtgatt cgcgaagggt tgcctttacc tgatttgatc
    19981 ttgatcgatg gaggaaaagg acaagtagat gtggcaaaag atgtcttaga aaatcaatta
    20041 ggaatcgata ttcctattgc tggcatggca aaaaacgata aacataaaac aaatgagttg
    20101 ctattcggta tagatttggc tgtagtaccg cttgagcgaa attcaccaga gtttttctta
    20161 ctccagcgaa tccaagatga agtccatcga ttcgcaatca ctttccatcg gcagctacgc
    20221 agtaaaaaca gttttgcttc ccgattagat gaaattgaag ggttaggacc aaaacgaaaa
    20281 aaaatgctgc ttaaagagtt caaatcgttg aaaaatatca cagcagccac gacagaagaa
    20341 ttacaagaaa tcggactgcc gaaaaatgtc gcacaaaacg tatttgataa attacatcag
    20401 gaatgaggaa agggcacttt acaaagaaag taaagtgtcc tatcatttta tgtacaacaa
    20461 aaagagcgtt tacgttttgt tggtaaataa taaatcgctt acgtctggtg tctaaggata
    20521 tatgtgattt cttgaagaag gagtgaagaa atgttagaag tcaaggatct tgttaaaact
    20581 tttgggaact acacagctgt cgatcatgtc tctttccaaa tccccgatgg aaaaatcatg
    20641 gggctaattg gccaaaacgg ggctgggaag acaacgactt ttcgtttgat cttggatttt
    20701 ttgacacaag accaaggaga agtgttatgg aatggccatc atcttgggga gaaagattat
    20761 gatgtcatcg gttatcttcc ggaggaacgg ggactttatc cgaaagtaac gattcaagac
    20821 caactgcttt attttgcaga acttagaggg aaatcaagaa aagagattga accaaaaatc
    20881 gatttttgga tggaaaaatt tcaggtaaaa gggaaaaaga cggataaagt aaaatctttg
    20941 tctaaaggaa atcagcaaaa agtccagctg atcgccacgt taatccatga acctaaattg
    21001 atcattttgg atgagccatt cagcgggtta gatccggtaa atgctgaatt gctaaaagat
    21061 ggaatattgg aattgaaaaa acatggttca tgtgtcattt tttctagcca caatatggat
    21121 aatgtagaaa aaatctgcga tcatttgatc atgttgcgaa acggaaaaat ggttttgaat
    21181 ggaaaagtac atgagattcg tgaatcattt ggccgtacga agttattctt ggaatctggt
    21241 ttaagccaac aggaagtaga agaaatcgaa ggcgtgaaaa aagtgatcct tcatgaagac
    21301 aatacgttag agattacctt agaagatcct gaagctggga aagaaatctt tactcgtgcg
    21361 actcagttcg gctacattcc tatgtttaac cagcaaccgc caactttgga agagattttc
    21421 aaattgaaag caggtgaaat cgatgcgtaa gttttggatt attaccaaag atgtctattt
    21481 aaaaaatgtt aagtcgattt ctttcttgat catgatatta gtccctttta tcatgctggg
    21541 tatcatttat gtagcaggaa actttgctca acaaaatagt gatgtcaata aaatcggcgt
    21601 attggctgat aatgagcaaa tcagtcagca aatgagccaa gtcaaaacag atgattttga
    21661 gttcaaagca gttgattctg aagaaaaagc acaaaaagag ctttcggaag aaaaaatcga
    21721 tgcgtattta gtagtctcaa ctgagaatga taagataaat gggatgcttt atagcgaaaa
    21781 ttcattagga caaagtacac aactactgat acaacaacaa ctgactggct tgcaatcgat
    21841 gcttcgagca agtcaattag gcatttcacc agaagaagta gctagcttga gccaacaagc
    21901 aagtttgact cgacaaaaag tcaattttga ttcaaaagga aaaatgacag ttggagaaga
    21961 taactcgaca gtccaatatg tcgtaagtta tgtagcaacg attgtcttgt tcattattat
    22021 tttgacgtat gcgcagatca ttgcacaaga gatcgcttct gaaaaaggca cgcggatcat
    22081 ggaagtgatc ctgtccagta cgactgcaca aaagcatttt tacggaaaac taacaggtgt
    22141 tttacttgtt gcattaacgc aaatggcatt atacggtatt atttttggta taggatacag
    22201 tcaattcaaa gatatggaaa tggtcaaaaa tatattagac ggtatctcat tggataactt
    22261 gattggacca ttcttgtggt attctcttct attcatgatc tttggcattt tgattttttc
    22321 tgttttagca gctttatgtg gctctttggt caacaaagca gaagatacag caaaagcaat
    22381 tttgccagtg acttatctat ctctaggcgg atatatgctt ggattgatac taggagcttc
    22441 agatccaaac aatatcgtta ttcggatcac ttcgtatatt ccattcttat cttcatatat
    22501 tatgccggtt cgcttagcaa atgagacagt cggtatgggt ggagcattga tttccttgtt
    22561 ggtacttgta gccgtaacat tcttattgat gatcttgtct gcaaacatgt acaaatcgaa
    22621 tgtactcgtg tatagcgaag gcggattatg gagttcattg aaacaatcga tttcaattat
    22681 gagaaatgaa cgaaaaaaag cataaaatat agaaaaatat agaaggaagc tggacattta
    22741 ttgttcccag cttctttttt gtatagacct atttctattt ttttattata ttggatatat
    22801 atattcattg aaaatagagc ataacaactt tataatgttg agtataccaa tttttggagg
    22861 cgttttaatg gaccgctatt tagatttact ttcagagaag ttccctacaa cggaagatgt
    22921 catcacggaa atcatcaatc tcgaagcaat tttgcagctt ccaaaaggaa cagagttatt
    22981 tttgagcgat atacatggcg aatttcctgc ttttgatcat attctgcgta tcggttcagg
    23041 aaatcttaaa gaaaagatca aagacctttt ctctgaacaa atgacggaaa cggagatcag
    23101 ccaattcact atttttacgg cttatccaga atatgtgttg acgactgatt ggtacaaaaa
    23161 acaagataaa tcccagctga tccatcgttt gatcgatctt ttacggttta cgacagtcaa
    23221 atacactcga tcaaaagttc gaaaaggatt gccgaaagaa tatagctata tcattgagga
    23281 actcttgtat atcgatgatc gtatcaacgg gaaaaaagac tatgtaaaaa aaatcattga
    23341 acaacttgtc ttgatgaagg aagaagaaaa atttttaaca aaacttgcgc agacgatcca
    23401 aaaatctgtc atcgatcatt tgcatatcgt gggtgatatt tttgatcgag gaactcaagc
    23461 agccaaagta atggatcgaa tcatggattt ttcctctgta gatatccagt gggggaatca
    23521 tgatattttg tggattggtg cgtattgcgg ctctgaggca tgtttattga cgcttttacg
    23581 gattgccgca cgctacaact atttatttga attagagaaa gaatatggat taaatttacg
    23641 tccattattt tcttttgccc atcaaaccta tcagaagaat ccagtattta cccctaaaaa
    23701 caggaaagat ctatctgaaa gagagcaaga agaattagaa aagatccatc aagcactttc
    23761 tatcctccaa ttcaaatcag aacatcagtt gttgaatcga cgacctgaat ttaaaatgga
    23821 cgatcgtaaa ttgttggaaa aaatcaacta tgaggaatca acgatcgttt tagaaggtca
    23881 gctctatgag ttgaaaggga cttgttttca aacgatccag ccagatgatc ctaaaaaatt
    23941 cttggaagag gaacaaaaag tgatcgatag tttgatgtat tcattccagc actcgttaag
    24001 aatgcaaaaa cacatgacgt ttttgatcga aaaaggggga atgtatctca cgaacaatca
    24061 gcatgtactg ttccatggct gtatacctgt agatgaaaag gggcagctct tagcatttga
    24121 attagaccaa tcttatcgcg gaaaagaact gctgggattt tttgaaaaac agataactga
    24181 aagcgcaaaa gatttgactg caaatgaaga tttggcgacc gatttgattt ggtatgcgtg
    24241 gagcgggcca ttttcgcctt tattcggcaa gagtaaaatg gcaacttttg agcgctattt
    24301 tctcacagag cctcatacgc acgtcgaaaa aagcaatccc tattatcact tgcgagatga
    24361 agagtggttc agtgaaaaaa ttttggcaga attcgatgca aaagaagaag gttcagcaat
    24421 catcaatgga cacacgccag taaaagtaaa aaaaggagag tcaccgatca aagcaaacgg
    24481 aaaagcattt gttattgacg gaggtctatc caaagcttat caaaaaacaa ccgggattgc
    24541 tggctactca ttactatgca attcttacgg tttccaaatc gtgacacatt atccgttctt
    24601 gcctattgaa caacaatttg caaaaaaatc tgatcaaacg aatgtaaaaa aagtgatcga
    24661 gcagcagctc ccacgcaaac ttaatcggaa tacgacaaaa ggaatggaat tacagcaaca
    24721 gattactcaa ctccaaaggc tgttggatta tcgaaaggac gattgagaaa aatgctagcc
    24781 aacattgctc aatagactaa aaagcgactg caacaaaagg cgtgtcgcag tctccttcgg
    24841 aattagctca aaaaacggta aaatattaga agctattttt ctgtttattt ctctaatact
    24901 caggtcaggg cgctgatttc acgacctctt ccttaaacgg tgttctcctg ccaactcctc
    24961 ggtcacaagt cacaatatcc aacaaacatg agaagctgtt ttttgaaatt gctccttgtg
    25021 actcagagtt gaacggcagg ctcacgacct aatcagaaaa acgattaaaa ttcattcaca
    25081 aagaaatcga ataaacgctg ttcagatgaa tctacacgat gagaaagttc cggatgccat
    25141 tggacaccaa ggattttgtg catattatct ttactttgga ctgcttcaat gagtccatcg
    25201 ttggaaaagg cgatggcttt taacaaagga gaaagatctt tgactgcctg atgatgatag
    25261 gaattcacaa agtatgaatc agggagaagg tcacttaata aactgtccga aacgattttg
    25321 acttcgtgag ttgcaaactg cggggcagtg ggctgttgtt cgtgttttac ggaccatttt
    25381 ggatataatg aaagatcttg ataaagtgtt ccctccaaca cgacattcaa tagttgaagt
    25441 cctcggcaga ctgcaaaaat cggttttctt tgtttcaatg cttccaagat caatgcctgt
    25501 tcgaattggt ctcgttgaat atttgtttcc gtcagttttg gatgagggtc ttccatgtaa
    25561 agttgcggac taacatcctg accgcctgca agaatgagtt tgtcgatttt ctcaattagt
    25621 tgaggcgctg aatctggaga catgactggc agaataagag gtaatccttg agcatctaaa
    25681 actgcatcca caaatccctg cggtgtatag cttacttgat ttccttggaa agtatctgtc
    25741 gctcgaatca gttgattccc ggcaatgccg atgattggtt tcatttttat tccccctaaa
    25801 gtagactaat tgtaacaagg atttaaaaaa atttaaacta tgtaagactt gcccctatac
    25861 ttatctgttc ttgctgtgtt actatctgta aagagaattt tcgtgtgaaa ttcatgacga
    25921 tagttgaaaa tattgcttaa tttgttataa taaaaagtaa gcagaaataa aaaggttggg
    25981 cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg acattttatt gaaggggcct
    26041 gcctatacta agttatcttt agaggtacat actttcaatg aaaaaagaaa gcatgtacct
    26101 cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa atccgaaaag
    26161 cagaagaaga aaatgagaag cgtgctcgaa ccctacggca gcagttgaag acctatgcag
    26221 aagaaaaaga gcaagaattg gagataatcc aaacgaacag tcggcaacag ctgaaacagt
    26281 tggtagaaga aaaagaacaa ttcgaaaagc aagaattaga aaaagaaaaa atgaaattga
    26341 aagcagaagc agacaaggtg caagcaactt tgaaagacca atatcaaaag catcagagtc
    26401 aagcaatcgg tgcaatcatt gaaagggtga aagaaactta tggccgtcac taagatggaa
    26461 aaagtgaccc tcatttcaga taaaagaaat caggagagga ttttacaagc agtacaagga
    26521 atccaaaatg ttgaaatccg ggatctgtat caaaatagaa caaacaacca gtgggtagaa
    26581 aaatattttc cagataccac gattattgat aaagaagcaa aattaagcgc tttaggcaat
    26641 cggctgaaag acatccaaga agctatccaa ttcatacaac accacggcga tagccgtcaa
    26701 aagaaactcc atttgaaaca tcaagaactt tccttacaag acttggaagc aaattattcc
    26761 gaagaaaatt tcatgaataa attagaagaa gtccttgatt taaaagaaaa gtgggaacag
    26821 ctaactgaaa aacgtgatcg tttagaagaa gaggaagaat ggcttcttca ttggcaacag
    26881 ctggatattg ctccaagtat gtatgcaagt aagacgacca tttttgagat ggggactgtg
    26941 agtgcagcaa attttgaaag tttcaaggaa gaactaagta agcttgatga cgtttatatg
    27001 gaagaaatag attatacgcc aaaacaagtt tatctttcgt atgttgcttt aaaaaaatca
    27061 gttgctgcta tagaagaaat tgctgggaaa tacgggttca ataaagaaac gtatccttat
    27121 gaacaaaatc ctaaagaaaa attgcaagaa accaaacagc aattatccat tgttcacgag
    27181 cagcaaaaaa agataacgac agcgttaggt cagtgcagcg gctatatcca agaatttgaa
    27241 tgggtagaag aagtcacact tgccgttgca gaacgggaaa aagtaaaaga acggtttatc
    27301 catgccactt atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa acagaatttg
    27361 atgcatgtgt taaacgaaac tgtatcagaa gaagatatat acgttagttt cgaaacacca
    27421 aactcagaag agattcagca ggaagtccct actaaattga aaaaccatcc gcttatcgaa
    27481 ccttttgaat tgctgactga aatgtacagt ctgccaaaat atgaagaagt agatccaacg
    27541 ccttggatga caccatttta tcttgtcttc ttcgggatga tggtcgcaga tgttggttac
    27601 ggattgctga tgctcattgg gacaatacta gctcaaaaac tatttgtttt accccgcggg
    27661 atgaaacgtt tcgtaaaatt ctttgagatt ctctcggtac catcgatcat ctggggattg
    27721 atctacagtt catttttcgg acaggcatta ccaaaagaac tatttggtat ccgcttgcct
    27781 ttcccaatcc tatctacgac ggatgacgtc aatacgattt tgattttatc cgtgattttc
    27841 ggtctgatac agatcttggt tggattattc gtggcagcaa aggaaaatat caggaggaaa
    27901 gattacttga gtgctgtcag tgatggtttt gcctggcaag ggatcctgat tgggatcgtt
    27961 attgcattga ttggtgtcat gttgataaaa aatacagcat ttgtttatct tggcgcgggg
    28021 ctatcgatca ttgctgcttt atgtatctta atcgtgccga ttatccaatc gccgtctaaa
    28081 gtcaagggtg ctgccaaagg agcgtataat ctttacggat tgactggtta tatcggtgat
    28141 ttagtcagtt atacacgttt gatggcacta ggtatttcag gcggaagtat tggcgcggca
    28201 ttcaatatgc tagttgcttt catgccgcca gccgctcgat tcagtgtagg gatccttttg
    28261 atcatcgctt tacacgcact gaatatgttt ttgacactgt taagtgccta tgtccatggt
    28321 gcacgattgc agtatgtcga attttttgga aaattttata caggaggcgg acgtgccttt
    28381 gacccgctga aaacggcgga aaaatacgtc aacatcaatc ataagaaaaa aaacaataaa
    28441 taattggagg atttttatac aatgatggat tacttaatta gtcaaaatgg tggaatggta
    28501 tttgcagtat tagcgatggc aacagcgacg attttttcag ggattggttc agctaaaggt
    28561 gttggaatga caggtgaggc agcagcagcg ttaacaacaa gtcaacccga aaaattcgga
    28621 caggctttga ttctgcaatt gcttccaggt acgcaagggt tgtatggatt cgttatcgcc
    28681 tttttgattt ttatcaactt aggaaatgat atgtcagttg tacaaggatt gaactttcta
    28741 ggcgcttctc tgccgattgc ttttacaggt ttgttttcag gtatcgccca agggaaagtt
    28801 gcagcagcag gtatccaaat tttagcgaaa aaacctgaac atgctacaaa aggaatcatt
    28861 tttgctgcga tggttgaaac atatgcgatt ttaggtttcg ttatttcctt cctattagta
    28921 ttgaatgcct aagcataaag gaggaaaagc agatgaacgc cattgaaaaa atcatctctc
    28981 agatgaatga agcagcagag caagaaagag cggcgttaga gcaagaagag cgaatgaaga
    29041 tcgatcaaaa ctttgaacaa aaacgtacgc aaatcgaaac agaacatcaa aaacaaaaag
    29101 aaaaacaaat cgaattactt gaaaaaaaat accgtcagct taggaatcgc caacaagtag
    29161 aagtacgaca agaaaatttg aatgcgaagc aggaattttt acgacgattg tttgcagatg
    29221 caatagctga aatggagaat tgggatgaat ctgaacaaat ccagtttatc aaaaattcat
    29281 tgtattcatt acctttgaca ggcaaagtaa cattcattgc tggagaaaaa tcagcagctt
    29341 atctttctca gacattgctg gatgaatgga acaatgaact tccctttatg atggttttaa
    29401 gtgatgaaac tgttgctgat caagcgggtt ttctgataaa cgatcaagga gttcagtata
    29461 attttctttt cagcagcctt gtacaagata tccaaggaac gatgagtttt gagattgcca
    29521 atcaattatt cgaataagga gggatcagtg tatggaatac cacgaactaa atcccttgat
    29581 tcgcggaaga gagcttgaac ttttgtccaa tgagacattt gaacggatga ttcaaacaaa
    29641 ttctgtcgaa gcacttggtg aaatattgaa atccacgatt tacagtccat atatttatga
    29701 cggatatgaa tatgacttcg aacagaatct tttaaaggag cagagccgtc tatttcgctg
    29761 gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc tatacgatgc gctatacttt
    29821 ccataatctg aaggttttga caaaagccga gatgacaaat caaaatctgg accatttgta
    29881 tatcgacgat ggattttatt cattagaaac cttgaaggat gccattcata cgcaagtttc
    29941 aaatgaactc cctgaaagca ttatggacag tatacgagaa gtacgtgatt attttgaaga
    30001 gtctaccatt cttcagggaa ttgacgtgat ttatgatcga cagtttttga cagagcagcg
    30061 acggcttgcc gataagctag gatatcaaga attgctgaca gaagtcattg cttttattga
    30121 cttgaccaat atcacaacga tggctcgagg aatattgcag aaaagaacat atgggttcat
    30181 gacgacggta ctttccagct cgggaagtat cgaaaaagaa gtatttttga actttgtaga
    30241 taaagatatg gaagattaca cgaattttct attagagaca gattatgctg attttctgcg
    30301 ccctgtcatc cgtgagggaa atcttgatct cgtaaagctt gaacagttga aagatgatta
    30361 tctaagttca ttgtatcaag aagcacaaac ccaggctttc ggaccactgc cactactagc
    30421 ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta tcggcaaaag
    30481 aagccatttc gaaccagaaa caatcagaaa gcgggtgaga caagtctatg acgcataaga
    30541 tcggtgtagt tggcgacaaa gattctgttt tgccattcaa attgtttggc tttgatgttc
    30601 gttatggtac aaccaagcaa accgttcgtc aggcaattga agaaatggca aagaatgagt
    30661 atggcgtgat ttatgtcaca gagcaatgtg cagcattgag cccagaaacg atcgaacgtt
    30721 acaaggaaca aatgatacct gctatcgtat tgattcccaa tcatcaagga tcattaggga
    30781 tagggctgga agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat atcttatagt
    30841 atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc ggacctcttg
    30901 taatggctga gaacatgtct gatgccagta tccaagacat gtgtctagtg ggagatctag
    30961 gagttatcgg agaaattatc gagatgcgtg gcgatgtggc gtctattcaa gtatatgaag
    31021 agacttctgg aattggtcca ggcgaaccgg ttcgctctac tggtgaagct ttatctgttg
    31081 aattaggacc agggatcatt tcacaaatgt ttgacggaat ccaacggcca ttagatacct
    31141 ttatggaaat cacacaaagt aatttcttag ggcgcggagt tcaattgccc gcattggacc
    31201 atgagaaaaa atggtggttc gaaccaacag tagaagccgg agaaacggtg agtgctggag
    31261 atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg gtgccaaatg
    31321 ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt cactattgat gaaacagtat
    31381 gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa tggccagtaa
    31441 gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc acccatgatc actggacagc
    31501 gagtgattga tacattcttc ccagtgacaa aaggaggagc agcagctgta cctggtcctt
    31561 ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa atgggccgat gtggacatgg
    31621 ttgtctatgt gggatgtggc gagcgtggaa atgagatgac ggatgtttta aatgaattcc
    31681 ctgaattgat tgaccctaat acaggagaat cgttgatgga acgtacggta ttgattgcta
    31741 atacatcaaa catgccagta gcagctcgag aagcttctat ctatacggga atcacaattg
    31801 cggaatattt ccgtgacatg ggttatgacg ttgctatcat ggcagactcc acttcacgat
    31861 gggcagaagc acttcgtgag atgagcggac gtttggaaga aatgcctggt gatgaaggtt
    31921 atccagctta tcttggttca cggcttgcgg aatactatga acgatctgga agagtcattg
    31981 cattaggatc agaaggtcgc gaaggaagta taaccgcaat cagtgctgta tccccgtctg
    32041 gaggggatat ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa gtattctggg
    32101 gtcttgattc cagtttagcc caaaaaagac attttccttc gatcgattgg atacaaagct
    32161 attctcttta ttcaacagaa gtagggaagt atatggatca tatccttcaa gctgactggg
    32221 catccatggt caccgagggc atgcgtattt tgcaggaaga acagcagctg aatgaaatcg
    32281 tgcgtttggt agggattgat tccctttcag ataatgaccg tctgacatta gaagtggcaa
    32341 aatccattcg tgaagattat ttgcagcaaa atgcttttga tgatgtcgat acattcactt
    32401 caagagagaa acaattcaaa atgttgaaga tgatcttgac ctttggtgaa gaaggacgcc
    32461 gtgcgttgtc gttaggtgct tatttaagtg agatcatgga cggtaccgta tcgattcgtg
    32521 aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt agaaaaacta gatcaaatta
    32581 ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga aggaggcatg agcaatgatt
    32641 aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga tggcggttga aaaagttgct
    32701 ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat tcgtcgtgga
    32761 caagtattgg aagtccaaga agataaagca atggtccaaa tattcgaagg aacaagtggt
    32821 atcaatttac gtgactcttc tgtacgattt ttaggccatc ctttagaact aggtgtatcg
    32881 gaagacatga tcggtcgagt attcgatggt ttgggacgac cgaaagataa cggaccagag
    32941 atcctgcctg aaaagcattt ggatattaac ggagaagtaa tcaatccagt agctcgggat
    33001 tatccagatg agtttatcca aacaggtatc tctgcaatcg atcatttgaa tacattagtt
    33061 cgtgggcaaa aattaccagt attttctggc tctggtcttc ctcataaaga attagctgct
    33121 cagatcgcgc gacaagcaac ggtactcgat tcatcagatg atttcgccgt agtctttgct
    33181 gctattggaa tcacttttga agaagctgaa tttttcatgg aagattttcg tcaaacgggc
    33241 gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg accctgcaat cgaacgtatc
    33301 gctactcctc gtatggcttt gactgctgca gaatatcttg cttatgaaaa gggaatgcat
    33361 gttttggtta tcatgacaga catgaccaac tatgcagagg cattgcgaga gatttctgca
    33421 gcacggcgag aagttccagg aagaagagga tatcctgggt atctttacac gaacttagct
    33481 actttattcg aacgagcagg aagaatccgc ggactaaaag gatcggttac tcaaattccg
    33541 attctaacaa tgccagaaga cgataaaact catccaatcc ctgacttgac tggttatatt
    33601 acagaaggac agatcatctt gactagagag ctttacaaga gtgggattca gccaccaatt
    33661 gatgtactcc cgtctctttc gcggttaaaa gataagggaa ctggacccgg aaagacaaga
    33721 gaagaccatg cagctacgat gaatcagctt tttgcagcct atgcacaagg aaaacaggca
    33781 aaagaattag ccgtagtttt aggtgaatct gctttatctg atgtagacaa gatctatgcg
    33841 aagtttgctg agcggtttga aaaagaatat gtcaatcaag gattctacac gaaccgttcg
    33901 attactgaaa cacttgatct agggtgggaa ctgctatcga tgttgccaag aaccgaatta
    33961 aaacgaatca aagacgacat gctggacaaa tatctgccag aagggaagtg agttctgatg
    34021 gcacgattga atgtgaatcc gacccgcatg gaattgactc gattgaaaaa acagttgaca
    34081 actgccacaa gagggcataa attattaaaa gataagcaag atgaactgat gcgtcaattc
    34141 atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc tatgacagaa
    34201 gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg aacctttcat cgaagaatta
    34261 tttgttttgc cggcggaaaa cgttgatttg tctatcgtag acaaaaatat catgagtgta
    34321 aaagtacctg tgatgaattt tgaatatgat gaacagctga cagacacacc tttggaatac
    34381 gggtatttga attccagtgc cgaactggat cggtcaatcg atcggtttac taccctgctt
    34441 ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc aactgatggc gagtgaaatc
    34501 gaaaagacac gtcggcgagt caatgcatta gaatatatga cgatccctca attagaagaa
    34561 acgatctatt atattcgtat gaagctggaa gaaaacgagc gcgcagaagt aacaaggttg
    34621 atcaaagtaa aaaatatggg taccactgac taagtggtat ctttttttaa aagaggaaaa
    34681 cgatgattaa acaaagcaaa caaataaaga aacgtttgcc ggctgttcag cttctcgcat
    34741 taggattttt tctcctgatt ttagttggcg gcggcctact taccctacca ttttttagta
    34801 atagtgggca tgggacaaat tttatcgatg cgcttttcac tgcaacttct gctgtctgtg
    34861 taacgggatt gacaacattg aatacagcag agcattggaa cagtgctggt caatttttga
    34921 tcatgctttt gattgagatc ggcggtcttg gttttatgat ggttccgatt ctatgctttg
    34981 cattgataaa gaagaaagtc agtctcagca cacgtattat attaaaagaa gcgctgaatc
    35041 tagaagaagt atcaggtgtg atgaagctca tgctttacat cctaaaattg gcctttgtta
    35101 tccaattgat tggtacggtt gctttatctt ttgtatttgt gcctgaattt ggctggttga
    35161 aaggtatctg gtacagtttg ttccatgcaa tctctagttt ttgtaatgca gggtttgatt
    35221 tgttaggaga cagtttagcc aatcaccagc aaaatgttta tttgatcatg gtggtatctg
    35281 ctttgatcat ttctggtggg ttaggcttca tcgtatggcg tgatttactg gaatatcatc
    35341 agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac cgttacaggt acccttttat
    35401 taggcggatt cgttgtgttc tttttcacag agcgcaatgg ggcaacgcta gtggaaggga
    35461 atatgtttca acgattggca aacacctttt ttatgagtgt gacacctaga acggctggat
    35521 attattcagt tgattacctc catatgagcc atgcaggtct gattttaacg atgtttttga
    35581 tgtacatcgg cggaacatct gggtcgacgg ccggcggatt gaaaacaact acgttaggtg
    35641 tgttgctgat ccagatgcgt tcgatgttga aaggacgtac gagagcggaa gcatttggcc
    35701 gtaccatccg acaaggcgca gtgttgcggg cacttacttt attttttgtt acgttgacgt
    35761 tgtgtgtagt cgccatcatg attttatcgg tgactgaaac gatacctaaa acttctggca
    35821 tcgaatatat tgcttttgag gtcttctctg cgtttggtac agtaggtctt acgatgggat
    35881 tgacgccaga cttgactgca atagggaaat tgatcattat agcgcttatg tacattggac
    35941 gagttggtgt attgactgtg gtcttctctc tgatgacaaa agcacaaaga acacaatcaa
    36001 aatacaaata tccagaagaa agtgtcatga ttggataatt tcatcttctg acagaaacct
    36061 agtgatttag taaaataaga aaataaaata atttttcttg cttcttggat actttaagtg
    36121 caaaatgttc gtttattttt tgaggcacta cccgttgttg aacctcgggt agtgcctctt
    36181 ttttattgaa aggaatcaat aagatttgat tcctttattt atcaataggt tataattcat
    36241 atttttttat tttttctttt acgatactta gtccagaagc taaaatttca aggttattag
    36301 aggataggga aattcgaacg aatgcatctg gttgcgcacg tgtagtaaag cgtctggaat
    36361 gataaagtcg gataccgtta gcaagaaagt cattctcaat tatcttaggg tcaatctcag
    36421 gatcaactgg aagaattcga tagtaaggga cacgattcgt tggtttttct actttgaaaa
    36481 gagaatcaaa aagctgattg gctctttttg taagacttaa tttagtttct aagatcctat
    36541 ttgcttcatt tgaatggagg atttgagtga tgacttccac atctaatcca gaagttttca
    36601 catttatatt aaaaattgct tgttctatct tgctttggac atgtactgga actacaagat
    36661 aagcaactcg caatccagaa caaataaatt ttgtcattcc agctaagtag attgtttggt
    36721 taggtaataa attttggaaa ggaggaatga tcgtatgttg atagtaagtt gttaaaaatg
    36781 aatggatatc atcttcaata acccatagac cttctttttc aatcacttcg gctaattgct
    36841 ctcgtctttt gtgagagatt tgaaacccta taggattatt acaagaaggc ataagaaaga
    36901 caccatgaat cttctttttt ctacattcct gaatcaatat atcaggtcgc attccttgct
    36961 cgtcaaaatc aattggaacg atctctaaat gatataactg agccaattca atgaaattag
    37021 cgtatgtata tcggtctgtt gctattctat cacctggaga gaaaagtgca gaaagtgtga
    37081 tagccaatcc attttgaaca ccggaaacaa tagatagaga ttctaaggac ggaatgactc
    37141 cttgagtttt taaccaattt gctgcagtag tcaattgaaa atttgtgcct ttgggatctt
    37201 ggtaattaag caaaccaaca agatttgggt tattactagt tgattgaata aacgggacaa
    37261 cgaatttatt acattcttca aaagaactaa ccagaccaaa atcaatgata tgttcggtta
    37321 cttcattggt agagatggtg atggactcaa agacattggg agaattaaat gtgccacttc
    37381 caatattcgt atacagcaaa cctttctcaa ttccatattt ataagccttg ccaacggtag
    37441 taaaatttaa atctaaataa tcagctagct ctctttggga gggtaactga gtgtttttct
    37501 gtaatttatt ctttaaaata tcttgttcta attgttgaat caacgatcga taataaggac
    37561 gcttcaattt ttgtttgtct ggtcgccaag ttaacgggta attgtcataa gaattaatag
    37621 gcattttctc cactccttaa aattgtatac catacaattt taccattgat tgtattgatt
    37681 ttagaagtta atcttttgtg aggagggaat tgtctatgaa aattattaat tttgaggaag
    37741 tagtagcttc ttttagtttt gaagaagcga ttcaagtaat gaaaaaatgt ttttttgatt
    37801 atgaaagtgg taaaatcagc caaaatcctc gattggtgga aattttacct gacggaaaag
    37861 gcaaaaatat gtttgcgatg atgcctgctt atttggggga gaacagatat tttggcacaa
    37921 aagtcattac cgcttttccg gataatcatc agtatcattt accttctcat ttaggagaga
    37981 ttttcttgtt tgatgctcaa aacggactac ccaaagcatt gataaacgca aatgcagtca
    38041 cctggattcg aacagctgct gtatctgcgc tggcaacaga ttacttagca aatccgcatg
    38101 cagaaacact tgctttaatt ggagctggtc aacaagcatt gtcgcatttg actgcgattt
    38161 ctaccattcg tcccatcact acagtgtttg tttacgatat tttagaagaa agacgaaata
    38221 cttttatcgc taaagctaaa aaacagtatc ctaaaataac gtttataaat gcaatatcag
    38281 tagaagatgc agtacgtcca gcggaaatca tttgtacgtt gacctctagt aaagaggcat
    38341 ttcttcaaaa agagtgggta agggaagacg cacatatcaa tgctgtgggc acatttactc
    38401 ctgatacaag agagataagt acatcgttga tggaaacttc tcaaatctat gtagatgatt
    38461 actcagctgc cttaacagaa agcggggacc tgcttatagc catggcagaa ggagatttag
    38521 aggaatcttg tattgttggt acattaggcg aattagtttc tgagaaaaaa acattttata
    38581 aaggagaatc gaggccgacg attttcgacg cagttggtct agctattgaa gatctttgtt
    38641 gtgcagaata tatttataac aaattagaga aaggggcttt aaaatgaact tagattatta
    38701 cgtagttgat gcatttgcag atgaggtgtt caaaggaaat ccagcagcgg tttatgtgct
    38761 agaagaatgg ctgccagaag gtacgatgca aaaaattgcc attgaaaaca atttatctga
    38821 aacagcattt acagtgaaga aaaaccaaga atttgagctt agatggttta cgccagatcg
    38881 agaaatcgat ctttgtggtc acgcgacatt agcaacagca tttgttttat ttaactatta
    38941 caaaatacct gatgaaacga ttaaattttc tactcaaagc ggtaatctat ttgtgacaag
    39001 aattaaagat tattattaca tggattttcc tagtattatg ccgaaaaaag tgccgatatt
    39061 gtctgaatat gaagaagcga ttggggctaa gattaaagaa gcctacttag cacgtgattt
    39121 atttttcgta ttagaagatg aaaaaacggt ggctaaattg cagccagatt ttacagctat
    39181 caaaaatttt gaattaggaa taggagtcat cgtgacagca gaaagtgtgc aaaaagactt
    39241 tgtttctaga accttctttc caaaattaac aatcaatgaa gatccagtat gtggatcagc
    39301 acattctaat cttattcctt attgggcaga aagaatgaat aaaacaaaat tgtcagctta
    39361 tcaaatgtca tcaaggggcg gggatctaga atgtgaatta aaagaagatc gtgtgataat
    39421 aggaggaaaa gcagttttgt tttctaaagg tacctcgttt atttgatttc tttcaaatga
    39481 attaaaaata aattggtgga gagtggcaac cttatagcta caatggttgt taaaaaaaga
    39541 cgcaaacttt ttcaatttaa tgaaaagttt gcgtctcatt ttaattattg gtgtttgttt
    39601 tttattcaat caagagtaaa atgccccata ctaactatcc tcggatgact tcgattttat
    39661 aaccatcagg gtcaatgaca aagtagtaag aaggcgcagt gccaggtaat ccttttaagt
    39721 cagtaatctc aagaccggca gctttatgtt gctcgtgtaa tttttctaga tcatcagctg
    39781 ctatagcgat atgaccatag ccgtctccta gatcatacgc accatgatca tagttgtaag
    39841 tcaactctaa ttcatagtca tccccaggaa gtgtcaaata aacaagtgta aatttgtttt
    39901 ctggaaaatc acggcggcgg ctttcttcaa aaccaaatgc cttttgataa aattcgattg
    39961 atgcttcaag atctttcact cgaacacatg tgtgagccat tttcatagat tcatctctcc
    40021 ttttcctttt actcaatcat aacaaaaaga agtaaggaaa tagttgattt gtgcttgttc
    40081 atgagaaatt tctcatgtaa aatggataga aaataaaact gttaaggtgg aaactattca
    40141 tggataaacc aatttctggt gaaaaaaagc aatttgctga ttatcaaacc cgttatgcag
    40201 catatattgt catagaaaga gatcagcaga ttgcagtaat cgaggctcca aatggtgctt
    40261 tttttcttcc tggcggggaa attgaaggag aagagacgaa agaagaagcg attgtccgtg
    40321 agctgataga agaaatggga attgtggcag aaatttcttc ttatctggga gaagctgatg
    40381 agtattttta ctcgaattat cgtgcaactt attattataa tccaggatat ttctttgttg
    40441 ctgattcttg gaagcgtata ggagaaccaa ctgaaaagac caataaaatt tggtgggtga
    40501 gccctcaaga agcgcttgtg aaattgaaac gtggtagcca ccgatgggca gtcgagagat
    40561 ggatcgaaaa aaataaaaac tgaaagaaag cgagtagatc agctttctct cagttttttt
    40621 atttattaca gtacatattt tttgattgct tcagctacac cgtgctcgtc gtttgatgaa
    40681 gtgatgacat ttgctgcttc tttgacaaga gaaacggcat ttcccattgc tactcctaga
    40741 ccagcatatt caatcatgga taagtcgttc tcgttatctc cgatagccat gatttcatct
    40801 tgatcaatac caaggtgctg tgcgagattc gcaactgccg cacctttact tgcttcttta
    40861 tttagtactt cgaagtaata gggcgcactt ttgaccgttg tgtatttatc acggaaagtt
    40921 tgcggtaaac gcgcaattgc tgcatctaaa atttctggtt cgtcgatcat catcattttg
    40981 acgatgttca tgtctggtgt catttcttca ggtgttcgat attttagcgg catcttgact
    41041 aaacttgctt cgtggatcgt ataagcgctg atatctcgat taggcgtgta gatcgctttg
    41101 tcatcgatcg aatggagatg actgccgaca cggcgtgcca tcacttcgat ctctaggaaa
    41161 tcttcatgtg tcagtccgtg acgggcgatg attttacctg atttcgtttg ttgtacgagt
    41221 gccccgttat aagtaataac gtaatcatta tcttgaaaaa ggttcaactc gtttaactgt
    41281 tcctggacac ctggtaaggg acggccagtg cataaaacga tcgaaacacc ttttgcaaca
    41341 gcagcggcaa tcgcttcttt tacttctttt gttacttctc ttttctcatt taataaagta
    41401 ccatcgatgt cgatagcgac tagtttgata gacataattt cctccaatat attttaattt
    41461 atttcaatca atgcgccatt acggatatgg ctggcaaatt cttgataagt ttcgtcaaat
    41521 aaatcataat gatcacgcaa ggacggatca accatttctt ttggaaagaa aaatcgctca
    41581 tctccttgtt cttgtcccgc taatgcagca acaagctgac taacctgtga taattctatc
    41641 aaagttccgt catttcgcaa aatctcgatt tgtgtgcgat gtctgccttg ttctgggcga
    41701 taaaaatcat aaggtagatc gtagctagaa ttcacagcag tgtaataaac aggattatat
    41761 ccgacctttt ctactaacaa agtgagttct tggactaatt ttgaatcccg gttgccagaa
    41821 aaagtagcag atttcagtgg tttacgattc aaaaaacgtt tagctaaatc atttaaaata
    41881 ggatcagcag attcagacca ttgagtaaag taagtgccca agaccccatc atcaagtttg
    41941 aggtattctt ctaaagtaaa gcttccgtca agaaatggca gaagtaattg agaatggagt
    42001 tcaaaactgc tggcatcttc ttgatacaac tcgtgtgcgc gatgaagcag atgctccaaa
    42061 attacttcca taccacggga aacaggatgg aagtacactt gcacatacat ttgatatcgg
    42121 ctaacgatat aatcttccac tgcatgcatg ccactcatgg aaaaagcgat cccgccttca
    42181 taaggacgaa tcacacgtaa tattcgggta agatcaaatg ttccatattc tgttccagtg
    42241 aaataagcat cccgaagcaa atagtccatt cgatctgcat cgatctgact agaaatcatt
    42301 tggacaactt gtgggttggg gtaagttttt tggatcacac tagctacctt ttcaggaaaa
    42361 ccttcttcta ctcggttaag aatctgatac acttctgttt ctggagaagt gatgatctct
    42421 acagtgatgg cttcatgatt cgtatggaag atatgttcaa atgtatgaga gtaaggacca
    42481 tgaccgacat catgcaacag tgccgcacat aaagtcacca gccgttcctg atcatcccag
    42541 cctccgttgc ctattttttc ttttgaaaaa tttcgagaaa agatatcgca gattcgtctt
    42601 gaaatctcat aaacgcctaa tgaatgagta aaacggctat gttctgctcc gtgaaaagtg
    42661 aaagaagaag tgcctaattg tttaatgcgt cgtagacgtt ggacttcttt cgaattgatc
    42721 aaatctaaga ttacttgatg ctggacatgg atatagttat ggactggatc acgaaaaact
    42781 ttttccatag gcagcatttg atatttataa ggggttgtca taatgtagct cctttatgtt
    42841 attacgttgt tccattctct ggatttggtt gttccattgc tccagctgga tttctttttt
    42901 tagccagtcg gtagaatcct gtaaatcact tgcagaatac agcttttcaa aagcttttgc
    42961 aaatgcttct ttgacttctt ttacaaaaag gggttggtca agcagttctt caagtgttgc
    43021 cattgtagca ggatccactg caggatagcc atttgtacca aagtcttctt ttaaagcaga
    43081 ttgataaaat cgcttcatga cttgcccgcg tttttcctga ttaccgttga cacttaagta
    43141 catcataaca gaaatcccat ttttgatccg acgctgagcg ataccggcaa attttttgcc
    43201 atggatgctt aaatcataag ttccaggaca ataagaatgg gtgatttcga atgcttgtat
    43261 cggtgccttt ggaaaagcgt atttagtcaa taggagcatt tgctcgtaag cgtcatctat
    43321 ggttattttt tttgtgtcac tttgggggaa aatcaaagaa atattcaaaa ttccttcatc
    43381 tgatacgatc cctaatccac ctgaatttct gatcactgga gcgtaacctg catctttcag
    43441 tgtttggatc ccgtcactta gaaaaggagt tcgaacatcc ttcataccta agatgactgt
    43501 gcagtcatat tgccagaaat gtaaaaagat ttgctggtgt tctttagaat attccgttaa
    43561 tatatctgtt aatgcaaaag gggaaaagtc gtttttttga taaatgtgct gatctagtcg
    43621 aatcgcaggg ttcattcaac cggctccttt gtataaattc atattttcta caatattata
    43681 ctctaaaatc aaagagttgt tttggaaaaa aggttttctg taaaaatgga caagttggtt
    43741 gacaaaacaa aagaaactag gaacaatgta gctatgagaa gtaaaggaga atgaataatg
    43801 gaattatcac aaggaacacc gatttcaatc aaattgcgta caaaagtaaa acagcaaggg
    43861 gaagtgcaag atttttattt tgatttgaaa gggcaaatgg tgacaattgg cgatacgctg
    43921 tacatacgtt ataaagagat tcaggaggag acgggcgatg agattcctgt aacgatcaaa
    43981 ttagttccag atggtcatgt acagctgatc cgtgcaggag aaatgcggat gcgtttgaaa
    44041 tttggctata aagaacggtt agaaacatcc tatcgaacac catacggtat gatccagatt
    44101 gctactttta cgaaagaact gcatgttagt ttgaaagacc gtcccacagg agggaaggtt
    44161 cgaatcgatt atgatctgtt catgggaccg gaaaaaatcg gcgaatatta tttgaccttg
    44221 gattttacag cgtaaatata aaagaaaagt ttgatttttt gtgtctgatt ttgtatgata
    44281 aggagtagac tgtgaaagga cgtgtcctga ttggaaatta gtgtatttga aggtgcaaac
    44341 aaaagcgaat tatcaatgat cgaagtagca cacgcgatct tagaacaacg tggcgatgtc
    44401 atggattttt ctgatttagc taaccagatc caaaactatc ttgggaaatc agacagtgaa
    44461 attcgtgatt cactcgcaca attttatact gacttgaata ttgacggcag ctttatctca
    44521 ttaggggata atcgttgggg cttgcgttca tggtatgcta tcgattcgat cgatgaagaa
    44581 gtaaaccatg gaatggacga agaggatgaa gatactccac gtcgccgtaa acgcaagaaa
    44641 gtcaacgcct ttatcaatga tgatgaagac gcgatcgatt acaatgacga tgacccagaa
    44701 gatacagatc tgacagaaga agatgacgat gatctgtttg atgacgatga tgatgaagat
    44761 gaagaaatcg ctgcttataa ttctgacttg caagaaatcg gagcagatga cccgacagat
    44821 gatgaagaag acttgccagg aaatatcgaa gaagatttaa gtatcataga cgaagacgat
    44881 gatgatgaag attatgaaga ggaagaattt tctgactctg aaaaagaata aacattattt
    44941 tcttacgaga ggttgtgaca gagacgatca gcctatcaga ataaggaggt gtgacacgat
    45001 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
    45061 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
    45121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
    45181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
    45241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
    45301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
    45361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
    45421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
    45481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
    45541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
    45601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
    45661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
    45721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
    45781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
    45841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
    45901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
    45961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
    46021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
    46081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
    46141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
    46201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
    46261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
    46321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
    46381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
    46441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
    46501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
    46561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
    46621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
    46681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
    46741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
    46801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
    46861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
    46921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
    46981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
    47041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
    47101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
    47161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
    47221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
    47281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
    47341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
    47401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
    47461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
    47521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
    47581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
    47641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
    47701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
    47761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
    47821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
    47881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
    47941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
    48001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
    48061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
    48121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
    48181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
    48241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
    48301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
    48361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
    48421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
    48481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
    48541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
    48601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
    48661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
    48721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
    48781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
    48841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
    48901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
    48961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
    49021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
    49081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
    49141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
    49201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
    49261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
    49321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
    49381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
    49441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
    49501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
    49561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
    49621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
    49681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
    49741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
    49801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
    49861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
    49921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
    49981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
    50041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
    50101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
    50161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
    50221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
    50281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
    50341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
    50401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
    50461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
    50521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
    50581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
    50641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
    50701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
    50761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
    50821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
    50881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
    50941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
    51001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
    51061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
    51121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
    51181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
    51241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
    51301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
    51361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
    51421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
    51481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
    51541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
    51601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
    51661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
    51721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
    51781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
    51841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
    51901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
    51961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
    52021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
    52081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
    52141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
    52201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
    52261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
    52321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
    52381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
    52441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
    52501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
    52561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
    52621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
    52681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
    52741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
    52801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
    52861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
    52921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
    52981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
    53041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
    53101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
    53161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
    53221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
    53281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
    53341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
    53401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
    53461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
    53521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
    53581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
    53641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
    53701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
    53761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
    53821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
    53881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
    53941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
    54001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
    54061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
    54121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
    54181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
    54241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
    54301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
    54361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
    54421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
    54481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
    54541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
    54601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
    54661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
    54721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
    54781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
    54841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
    54901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
    54961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    55021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    55081 cttagttaag taatcgaact ctctacttgc tgaaatatca gcaggtagag agtttttttt
    55141 gcaactagct tctgtatatg atgtttacaa agaatggtac aagaaaagat catccttgaa
    55201 aaaggaaagc taacaataac tagcatcata taagagcttt taaggataga acagtagaat
    55261 aggaatctat gagcaagtcc tctcgtataa ataaaatagg agggaagtag cagcatattt
    55321 ttcataaagt ggctctcttt cttcattttt actttggttg ctcctatttg aaaattcgag
    55381 cacactcaaa tacaataaaa agaaatggca agggagagga ttagatgaaa aaagaagaaa
    55441 aaatcgcgat tttacaagag atcatccgaa ttaaatcagt gaatggaaac gaaggtgaag
    55501 tagcagccta tttgaataag cttttggcaa agcatgacat aacaggagag attgtttctt
    55561 acagagatgg tagagataat ttgattgctc gttaccaaaa gggccaatca ggaaaagtgt
    55621 taggcctatc tgggcatatg gatgtcgtgg cggctggtga tgaatcgtct tggacctatg
    55681 caccctttgc agcagaaatc catgggaacc gtttgtacgg acgtggtgct acagatatga
    55741 agagtggctt ggcagcaatg gtgattgcca tgatcgaact gaaagagtca ggaaaaccat
    55801 ttaacggaac agtcaaatta ttagccactg tcggagaaga agtgggtgaa ctaggtgggg
    55861 aacagctcac gaaagcaggc tatgtagatg acttagatgc cttgatcatc ggagagccaa
    55921 ctaattacag tttgatgtat acgcacatgg gatcaatcaa ctatacagtc acctcgcacg
    55981 gaaaagaagc gcatagctct atgccagacc aaggttataa tgcaatcaat cacctaaatg
    56041 aattcattac gaaagcaaat gctgaaatga atcacctcgc tgaaacaatc gagaatcctg
    56101 tcctaggaaa aacaatccat aatgttacat tgatcagtgg cgggaatcaa gtcaacagca
    56161 tcccaagcca tgcgcagctc caagggaaca ttcgatcgat tccagaatac ccaaatgata
    56221 aaatcattgc attgctgcaa tcaatcgtca atgaattgaa tcaagagaca gactatcatt
    56281 tggaattgat gatcgattac aacaaaatac ctgtcaaagc tgatccagat tctcctttga
    56341 ttcattgcat tcagcaacaa ttcagccagc cattaccttt agttggagcg gcagcaacaa
    56401 cggatgcagc cgaatttacg aaagctaacc attccttcga ctttgtcgtc tttgggcctg
    56461 gtgtcgttac tttgcctcat caagtagatg agtatgttga aatcgataat tatttggata
    56521 tgattgaaaa atatcaagga attattttat cctacttagc gtaagatttt tttaataaat
    56581 gaaaaaacag agccagatta gcattatttt gctaatccag ctctgtttat tcagcctatt
    56641 tttctttttg aatatatcaa gcgttgtggc aacatagtga tgactaatga aacaacgacg
    56701 atagacaaca aacgatcaag gtagtctgta cctgcctgta ccaagatcac acttgttgtc
    56761 tgacttaaac ctaacccatg taatagctga acgatcatac tggatcctga ggaggtgatc
    56821 ccgccaaata actgaacggt aatgatcgaa gaaacgatcg ttccaggtac cgacaaaatc
    56881 aaagcgagta ataacagttt caagctgttt ttatctttgc cgagatatct tggcaaaatc
    56941 aatccagcaa gcaagcctgt gagaatttgg attggcgaat aatacagaga aaaaatatct
    57001 gtagtcactc cacttaaaat accgctggac aatcctgtca gcattccagg tacaggccca
    57061 agcaatgcac cagcaaaaat cgttccgata gaatcgagat agattggcaa acgaagccat
    57121 aaagcaatcg ttcctccaat ataattgatc ccaatacaca aagccatcac tgtaagtacg
    57181 cgagttgcca ttttgttttt attcattttg ttagcccatc tttaaccttt ccaaatcttt
    57241 cataatcgtt tcttcttgtg catttaaaag aacggttaaa aactttcgga aaaaaagacg
    57301 agtatctact tttgtcataa ttttactgtt agcaggtttc tgccaaaatt cataacgatc
    57361 aaccaatgtt tgtccacgac ttattccttg agtttccaca gcagtgaagc ttttgaaacc
    57421 ttcacagatg gccccctcga taaaataagc aacagctaac ggatcattga tgacacatcc
    57481 taaaattctt tcttgtttcc agtggaagtc aaaataaaaa cgagtgatcg cttttagata
    57541 ctctccctct tcaggattca tttgacagca gtattcgagg atagtcggtg tcaagacgat
    57601 ttctcttgtc acatctaacc caaccatttc tatcgtttgt ttgagattct cgaatacata
    57661 agaagctgct tcaggatcac accagtaatt gtattcagca actggcgaac agttaccatg
    57721 gcttttgtaa gttccaccca tcgagacaaa acgatccata tgtttcccaa tatttgggtt
    57781 gactttcaaa gcaaaagcga tattggtcaa aggacccaaa gcaatgactg atgtatttgt
    57841 cttttctttg aaataatcag ccaaaaaatc aacggcatgg agaggttctg cctgttttct
    57901 caagatcatc gggaaatttg tttcccctaa accatccata ccatgagtgt cttgtgcact
    57961 gatgaaaggt tgcttcaaag gtttttccat tccttgataa acaggaatgt ctaaacgatc
    58021 acagcgttcc aggcatttca gcacattctg cgtacctata tctgcaggga cattcccaca
    58081 aactacggta atagcaacta cttcgatatc aggggatttg acagctaatg ataaagctaa
    58141 agtgtcgtcg atccctggat cgcaatcaat aattactttt cgcattattt ccaatctcct
    58201 ttctttagag atacttagtt ttttattctg ggaagtttcc gaaccagtcc aagacgagcg
    58261 tcttgattat aatgcacgaa attagtatac tggcttttat tgcgaatgta aatgctctct
    58321 tacaagtaaa tctattgagg agaactttaa gtaaatgata caatgaaaaa gaaatggaat
    58381 ttacgtgtgc cgaaaataaa gaatcctatg gatcggtaca ttggataata gttagattat
    58441 agtttggcag aaagcggcgg gtgtacaatg gttatttttt atttcattct tattctgata
    58501 attataggga tcagttacca ttttctttct tatttttatg caaagaaatt agtaagagcg
    58561 ggagtacaga gaggatatga ctggtatgaa gacactggtc atcgtttgat ggaccctgct
    58621 gcagtcacgt cagtagaaaa aaaacgacaa aaattagaag aagagtgtga aagcttttgg
    58681 gagcagggaa cacccaaaac aatcaaaagt tatgacgggt tgaaactagc tggtcagatg
    58741 ttcatccatc caaataagca gaataaatgg gtcatttgcg tacatgatta ccgaagcaca
    58801 gggaaaagag acatgtctca tataggtaaa agatatgcag agaaaggatt caacgttctt
    58861 gtacctgatt tacgtgcgca cggagaaagt gaaggagaaa tcatcggaat gggatggcta
    58921 gatcggctag atttaattgc ctggatccaa ctcatcttag atgaacagcc gaatgcgtcc
    58981 attattttac acggcggatc aatgggagct tctacgatca tgatggcaag tggtgaaaaa
    59041 cttccatcag cggtcaaagg atttatttta gacagcggat atgtttctgt ttatgcagaa
    59101 ttccgatata tgttaagtaa aatcactgtc tttcctaaaa aaatggtcat gagatatgcg
    59161 aatcattatg cccagaaata cgccggttac tccttaaagc aagcatcagc tacgagacag
    59221 cttggcagca accatctgcc tttattggtc attcatggtg aaagagatca ttttgtccct
    59281 acagaagcag cgtacacgat tcaaaatgct acggcagggg ataaagcact gctgcttgtt
    59341 ccagaagcag aacacttgga agcatcgatg aaagatccga aaacctattg gacagtgatt
    59401 ttttcattta ttgaacaaag aataacgatg aattaatttt ctcttctttt ttgtaagtaa
    59461 ttcgaggata aataattgtt tatagatcag gagactgcta aaataaggta ggaataaaaa
    59521 aatatcagtt atttgaggag tgcggcatat ggaaatcaaa gaagaaaaaa atcgtcttgc
    59581 tttattcaat gatgaacaac aagagatcgg agaaatgaca tggtcagatg cgggcccaga
    59641 catcatgatc attgaccata catttgtaga tccagcatat cgtggtcaaa agttagctga
    59701 aaagctagtg tatactggag tagaattagc aagacgagac gggaaaaaga tcattccttt
    59761 atgcccttat gcaaaaaaag agtttgaaaa gaaaccagaa tatcaagacg tcttacgtca
    59821 gtcataagaa taacagctga aagttttgaa ctttcagctg ttattccatt tatatagagt
    59881 tgtcacggac ttttatctat gataaagtta aagagacgat atcttttggg aaggaggctg
    59941 cgatatgtat aatttaattt tgggaatcgt gatcgtttta tcgattgtta tggtcattgc
    60001 gattatgatg cagccaagca agcaaaatag tgcagcgagt gcatttaccg gcggtgcgga
    60061 tcaactattc ggcaaacaaa aagctcgcgg ctttgaagct gtcatgcagc gatcaactgc
    60121 agttatggga gctatctgga tgatcttatt atttgtccta gcatatttat cttcgaagta
    60181 gttgaataag cctctcgcaa aacggcgggc ggcttttttt attgcaaaga gaacgcttaa
    60241 gaagaagtta tgaaagaaac tatggtaaaa tgaggcgaaa gaggtgagac gagtgagaca
    60301 attaccaaaa cccctttatg caaaacatgg aaagcgagcg gttttgctgc ttcacgctta
    60361 ctcaggaagt ccaaacgatg ttcgaatgct cgcacgattt ttggaaaaag cagattatac
    60421 agtttatgcg ccgcttttta aaggacacgg aacaatggag ccatatgata ttttgcaaga
    60481 aaaagcagaa agctggtggg cagacactaa aaaagccatc cattttttac agtctgagca
    60541 gttttctgat attgccgtat taggattatc aatgggcggc atctttgctg tacgagcact
    60601 agaagaagaa tcagtgattg gcggcggttt tttctgttcg cctttgtctc ctgtaaaaac
    60661 aaacgtgcct gaaaactttg aaaaatacgt acgtcaagtg ctgaagacag caggtaaatc
    60721 agagaaagaa atcaatgaaa aagcagttgc ttaccgctcg ttagctgaac aacaattaat
    60781 ggatatacaa gatcaggcag caatcgtaga aagtaggttg tcagatatcc aacagccgat
    60841 atttttagct caagctggga aagatgaaat gatcgaccct aatggcgtat ttgaaactgc
    60901 acgcaagttg agccgtcagc gagtgacact tcgatggtat cctgaaagtg gtcatgtgat
    60961 tacagttggt acagcaagac gcgaattaga aaaagacgtc ttggaatttt tagaaaaatt
    61021 accttggaat gaggaataaa tatgacaaaa gaaacgataa aagagaaaat tctctttttc
    61081 atggaaagca gtcgcaaacg cagtttttct atggaagaaa tcgctgaagg tttagggttc
    61141 caaaaaagcg atgattttaa attactagta caaacgattg ctcaaatgga aagagaacag
    61201 agtatagttt tcaataaaaa aggaaaagtg aaacttcctt taaaaccaat cttgatcgaa
    61261 ggaacatttc gagcaaatga acgaggattt ggatttgtaa cgattgatcc cgaagaagat
    61321 gacatctata tcccaaaaga agcgactggt tttgcaatgg atggagacac cgtagcaatc
    61381 gatatcatga agcgagcaaa tacagatatg gatcgcggag cagaggggaa agtagtcgag
    61441 attcgagacc gggcgactac tcagttagca ggggaattcg ttgcttattc agaagaagaa
    61501 atacaagaaa cggatcttta cggcgttgtt atcccgaaag atagaaagct caaccaattt
    61561 aaagtatatg cagcagcaga gggagttcgt ccagttgacg gaagtattgt gctgatcgaa
    61621 ctgacccatt accctgaaaa agggtatgcg actagtttag aagggataat caagcaagtg
    61681 attggtcata agaatgaccc tggcatggat atcctctcaa ttgtcgtggc taatggtatc
    61741 cctacgaaat ttcctgatga tgtattgtct gaagcagatg aagttccaga tcaaatcagc
    61801 gaaaaggata taactggacg tagagattta agaaatcagc tcattgttac gattgatgga
    61861 gaagatgcaa aagatctaga tgatgcagtt acggttaaaa aattagataa cggtaattat
    61921 tttcttggag tacacattgc agatgtttct tactatgtga cacaaggcag tcaattggac
    61981 agagaagcat atgaaagagg gacaagtgta tatttgactg atcgagtgat tccgatgatc
    62041 ccgcaacgtt tatcgaatgg tatctgttca ctgaatcccc atgttccaag gctgaccatg
    62101 agttgcgaaa tggagattga tccaaatgga caagtagtct ctcatgatat tttcccaagt
    62161 gtgatccaaa caacagaacg gatgacctac acagctgtca atcaaatctt agaagatcaa
    62221 gacgaagaaa cgatgcaacg ctatgctcag ctcgtaccta tgttccaaga aatgggtgaa
    62281 ttgcatcgaa tccttgaaac gatgcgaatc aaacgtggcg cgatttcttt tgaggatcgc
    62341 gaagcaaaaa tccttgttga tgctgaagga cacccaaatg atatccagct aagaagcaga
    62401 ggaattggtg aacgcttgat cgaatctttc atgcttgcgg ccaatgaaac ggtagctgaa
    62461 cattataata aattaaatct gccgtttatt taccgaatcc atgaacagcc aaaagaagaa
    62521 aaaatgcagc gcttctttga ttttgcttca gctttgggca ttctggtccg aggaacgaaa
    62581 ggaacgatca caccaaaaga tttacagcat gtgatcgaag gagtagaaga caaaccagaa
    62641 gctgcagtta tcaatactat gttgctaaga agcatgcaac aagcacgcta ttcagaagac
    62701 aattttgggc attatggctt agcggcagat tactacacac actttacatc tcctattcga
    62761 cgctatcccg atctaatcgt ccatcgtctg atccgttctt acagtgaaga taaaagtgaa
    62821 agcaatcaaa gttcgtgggc tacggaactt cctgaaattg cagatcatag ttctaagatg
    62881 gaacgtcgtg cagtagaaac agaacgtgaa gtagattcca tgaagaaagc agaattcatg
    62941 atggacaaag tgggggaaga attcgatggg atcatcagct ctgttgtgaa attcggcctg
    63001 tttatcgaac tgccgaatac agtagaggga ttgatccata tcaatgaatt gaaacaggac
    63061 tacttccatt ttgttgaaaa tcatttagct cttgtaggtg aaagaactgg tctgactttt
    63121 aaaatcggac agaaagtgcg agtaaaagta gtcaaagctg atccagatga acgagcagta
    63181 gattttgaac ttgtttccgc agaagaggtt acagcgttgg aacggccaaa aacacgaaat
    63241 ggaaaatcag gaaaacgcga aggaaaacaa gcaggaaaac gcccagaaag aaatggcaag
    63301 aagaacaaac gatatagcga tagtaagcaa acgttcggta agaaaaaagg gaagaagcca
    63361 ttttacaaag gtgttaaaaa gaagaaaaag aaaaaataaa atgagttgga ggaaacagct
    63421 atgccaaaag gcgagggaaa attaattgca caaaacaaga aagctcgcca tgattattcg
    63481 atcatcgaca cgatggaagc agggatggtc ttgcaaggaa ccgagatcaa gtcgatacga
    63541 aacagccgga tcaatctaaa agatggattt attcgcgtcc gcaacggaga agctttcttg
    63601 cataatgttc atatcagtcc ttatgaacaa ggaaatattt ttaatcatga tccgttgcgc
    63661 acgagaaagt tattattaca caaaaaacaa atcatccggc ttgaaaatga attgaaaaat
    63721 actggaatca ctgttgttcc tttaaaagtc tatattcgta acggctatgc caaggtattg
    63781 attggtctgg cgaaagggaa aaaatcttat gataaacggg aagatttgaa acgaaaagat
    63841 atcgatcgac aaattgatcg aacattaaaa aatttctcta gataatttca ttatcctttt
    63901 tcgcatattc ggtgcttttg cttttactta atgataaaat ttgttagcat ttaatctgat
    63961 aaaacatgat tatcataggt tttttcttag atcaggagca ccgtgtataa agaaaaattc
    64021 gcttcgtttt tgactgaaat aaaaaagtgt ctaattttca tgaaaatgtt ttgaattttc
    64081 tgtttcatgg tggtatgata ccgtgtggtt agaaaccgat tggaaaaaac cagatacgca
    64141 catgaattga ttttatgtga atttcgggga aaagaggtga aatggattgg atgaacgctc
    64201 gctaacgttc cacattggac caatttggtt tgatggtaca gtttgtataa tggtgttact
    64261 gacctgtttg attgttttct tcttagctta cttttttaca agaaatctta agttgaaacc
    64321 aacaggcaaa caaaatgcga tagaatgggt cattgatttt agtcgaggaa tcgtaacaga
    64381 taatctgcct agaaaagaat tgagcaattt ccatttgttg gcatttacat tgtttttatt
    64441 tgtttttgtt gcaaacaaca tcggtttagt aacaaaaatc gttttaccta acgaaacaac
    64501 actttggaaa agcccgacag cagacccgtt tgttacttta actttggcat ttataatgat
    64561 cttacttact cacttatttg gggtgaaaaa gttaggcttc aaaggatatt tcgtaaattc
    64621 gttcttgaaa ccatatagtt ttatgtttcc gatgaaactg attgaagaat ttacaaactt
    64681 attaacactt gctttacgtc tttatgggaa tatttatgcg ggggaagttt tgctaacctt
    64741 gattgcaaaa atggtaataa gcttaggctg gttctcattg ccgttagcaa ttccattaga
    64801 gatggtctgg atcgcattct ccttgtttat cggaagcatc caagcatttg tcttcgttac
    64861 tttatctatg gtttatatga gccataagat tgaagtggaa gaataatatt cgttttaaaa
    64921 acaaactact atttatctaa tttaggagga aaacaattat gaattatatc gcagcagcaa
    64981 tcgcaatcat gggagcagca atcggagccg gttatggtaa cggtcaagtt atctctaaaa
    65041 caatcgaatc aatggctcgc caacctgaaa tgtctggtca attccgtaca acaatgttta
    65101 tcggggtagc tctagtcgaa gctgttccta tcttaggggt agttgtcgcc ttgattctag
    65161 tttttggtgt ttaattgaat cagtattaag gcagaaacaa tttggtttgt tactgctttt
    65221 ttacatgaaa actagaagta cttcaaagaa aggaaggctc gcctatgctg aatcaattgg
    65281 caattgcaga agttggcaat gcgatgttag gaaatgtcat cgttgtcagc ggctcatttt
    65341 tgatactact ggctctcttg aagcattttg cttggggacc aatcagcgat attttgaaaa
    65401 aacgtgaaga caagatcgcc aatgatttgg attctgcaga acaatctcgc atcaactcag
    65461 cgaaaatgga acaagaacgc gaacaacaat tgttagcctc tcgttctgat gcagctgata
    65521 tcatcaaaaa tgcgaaagaa agtggagaat taagccgcca aaatattttg aaggatgctc
    65581 aagaagaagc agctcgtcta aaaagtaaag cccaagctga tatcactgta gaacgtgatt
    65641 cagcgctgaa ctctgtaaaa gacgatgttg cagaactatc tcttcaaatc gcggaaaaaa
    65701 tcttgaacaa agaattatct ccagaaatgc acgaatcatt aatcaatcaa tatatcgaag
    65761 gtctaggttc ttcaaatgaa actagataaa tatacagtag gaagacgtta cgggaaagct
    65821 ttatttgaac tggcgatcga ttcaaataaa gcagaagaaa tttatcaaga tctcgtaagc
    65881 ttacgtcaaa tctatgaaca agtacctgga ttaggtaata tgttaagtga tgttcgtcta
    65941 gaacctcatg aaaaacgaga aatcatggac aagcttgtta gtggctatga tggaatcgtt
    66001 aaaaactttt tagaagttgt ttatgcgtat aaccgaatgg atgatctttt gttcatgatc
    66061 gaagaatatg aacgtcgata tgatgaacat caaggtttgc tgttgggaag tgtcacaaca
    66121 gcagttccac tttcagacga acaacgaata caattagaaa agaatgttgc aaaaacaatg
    66181 aactatcaat cagtcgaatt aaaacaaatc gttgattctt ctattattgg tggcgcgatt
    66241 gttgaagcta accatcgagt aatcgacgga agcattcgca cgcaattaga aaatatgcgt
    66301 aatcaattaa atagatagta caaagaggta ggtgaatcga atggccatca aagcagaaga
    66361 aattagtgcc ttgattaaag aacaaattga aaattatcaa aacgtgcttt cagtcgaaga
    66421 aatcggtacc gtaacttatg taggtgacgg gatcgctcgt gcacatggtt tagaaaacgc
    66481 gatgagtggt gagttgcttg aattttcaaa tggctcatac ggaatggcgc aaaacttaga
    66541 atcaaatgat gtaggtatca ttatccttgg cgatttcgag tccattcgtg aaggagataa
    66601 agtaaaacga acaggaaaga tcatggaagt tccagttgga gaggccttga ttggacgggt
    66661 agttaatccg ctaggtcagc caattgacgg actaggtgaa atcgttactg acaaagctcg
    66721 tccagtagaa gcaatggcac caggcgttat gcaacgtaaa tctgttaacg aaccaatgca
    66781 aacaggtcta aaagcaatcg acgctcttgt accaatcgga cgcggccaac gtgaattagt
    66841 tatcggtgac cgtaaaacag ggaaaacttc cattgcgatc gatacgatca tcaaccaaaa
    66901 aggtcaagat atgatctgta tctatgtagc aatcggacaa aaagattcta cagttcgtac
    66961 acaagttgaa acattgaaaa aatatggcgc aatggattac acaatcgttg tgaatgccgg
    67021 tgcgtctcaa ccagcgccgt tgctttatat cgcaccatat gctggtactg caatgggtga
    67081 agagttcatg tacaacggga aacatgtctt gatcattttt gatgatcttt caaaacaagc
    67141 tgttgcttat cgtgaacttt ccttgctgct tcgccgtcca ccaggtcgtg aagcttatcc
    67201 aggggatgtt ttctacttac actcacgctt attggaacgt gcagcaaaat taagtgatga
    67261 tttaggtggc ggttcaatga ctgccttacc atttgttgaa acacaagctg gagatatctc
    67321 tgcttatatc ccaacaaacg ttatttcgat cactgatgga caaatcttct tagaaagcga
    67381 cttgttctac gcaggtactc gtccagcggt tgatgccgga ctatctgtat ctcgtgtagg
    67441 gggttctgcg caaatcaaag caatgaaaaa agttgctggg actttacgtc tagacttagc
    67501 aagttaccgt gaattagaag catttacaca attcggttct gacttggatg cagcaacaca
    67561 agcaaaacta aatcgtggac gccgtacagt agaaatcttg aaacaaaaat tgcatgcacc
    67621 gctagctgtt gaaaaacaag tagtgatttt gtatgctttg acacatggat tcttagatag
    67681 tatccctgta gacagtattt tagacttcga acatgaatta ttcgaatatc tggataccaa
    67741 tcattcagat atctttgaaa caatccgtac aacaaaagat cttccagaag aagaagcgtt
    67801 gaacagtgcg attcaggaat ataaagacat gttcttagct acaaaaggaa ataattcctc
    67861 tacagaagat aaattgaaat ccattcaaaa tgcatagtga ggtgagatga atgggtgctt
    67921 cattaaatga aatcaagaca cgtatcgctt caacgaaaaa aacgagccaa atcacgcggg
    67981 caatgcagat ggtgtctgct tctaagttga caaaatctga agcttcttca cagaagtttc
    68041 aggtttatgc aacaaaagta agagaaatcg ttacgcattt gactgctact cagttaaatg
    68101 atattgcctc agaaaatcct agaggggata tcaattacaa cagcatgttg ctcagccgtc
    68161 ctgttaagaa aacgggttat atcgtcatca ctgcagatgg cggtttagtc ggtggatata
    68221 acagctcgat attgaaacaa acgatgtcta ttttagaaga agaccaccaa tcacccgatg
    68281 attacgtgat gatcgcaatt ggcggaactg gtgcagactt ctttaaagca cgcggtatca
    68341 atctagcata tgagctgcgc aatctatctg atcagccaag ctttgatgaa gtccgcaaaa
    68401 tcgtaaatat ggcaacaact atgtaccaaa acgaagtatt cgatgaatta tacgtatgtt
    68461 ataaccatca tatcaattcg ctaactagtc agtttcgtgt agaaaaaatg ctgccaatct
    68521 cagacttaga tccagaagaa gcaactactt tcgaacaaga atatattttt gaaccttcaa
    68581 aagaagaaat cttgtctcag ttattgcctc agtatgctga aagtttgatc tacggtgcga
    68641 tcgttgacgc caaaacagca gaacatgctg ctggtatgac agcaatgaaa acagcaactg
    68701 ataatgctgc taatatcatt gatgagctga cggtttctta caaccgtgca agacaagggg
    68761 ctattaccca agaaattacc gaaattgttg ccggagcttc ggcactagaa tagtattgag
    68821 attggaggaa aaaagatgag ttcaggcaag attgttcaag taatcggtcc cgttgtcgac
    68881 gtggaatttt ctttagacca atccttacca gatatcaaca acgcattagt tgtttataaa
    68941 aatgacgaaa ataaatcaaa agttgttctt gaagcagcgt tagaattagg ggacggagtg
    69001 atccggacca ttgcgatgga atcaacagat ggtttacaaa gaggaatgga agtcattgat
    69061 acaggcaaag caatctctgt tcctgtaggt aaagaaacat taggtcgcgt attcaacgta
    69121 ctaggagata cgatcgattt agaaacacct ttcccagaag atgcggaaag aagcgaaatt
    69181 cataaaaaag caccagcctt tgacgaatta agtacaagta cagaaatttt ggaaacaggg
    69241 atcaaagtta tcgatttgct tgccccatat ttaaaaggtg ggaaagtcgg actattcggt
    69301 ggtgccggtg ttggtaaaac cgtactgatc caagaactga tccataatat cgcccaagaa
    69361 catggtggta tttctgtatt taccggtgta ggtgaacgta ctcgtgaagg taatgacttg
    69421 tattatgaaa tgaaagattc aggagttatc gaaaaaacag ccatggtgtt cggacaaatg
    69481 aacgaaccac caggtgcacg tatgcgtgtt gctttgactg gtttgacgat tgcggaatat
    69541 ttccgtgatg tagaaggtca agatgtactg ttgtttatcg acaacatttt ccgtttcact
    69601 caagctggat ctgaagtatc agccttgtta ggacggatgc cttctgcggt tggttatcaa
    69661 ccaacattgg caacagaaat gggtcaattg caagaacgta tcacatctac gaaaaaaggt
    69721 tcgatcacat cgatccaagc aatctatgtg ccagccgatg actacactga cccggcgccg
    69781 gcaacagcat tcgcccattt ggatgcgaca accaacttgg aacgtaaatt aacagaacaa
    69841 gggatttatc cagcggtaga tccattggct tcttcttcaa gtgctttagc acctgaaatc
    69901 gtgggagaag aacattataa agtagcaaca gaagtccaac acgtattgca acgatatcgt
    69961 gaattacaag atatcattgc tatcttaggt atggatgaat tatccgatca agaaaaagtg
    70021 ttagtttctc gtgcccgcag agtccaattc ttcttgtcac aaaactttaa cgtggctgaa
    70081 caatttactg gtcttccagg ttcatatgtc cctgtagaag aaacagtgaa aggattccgc
    70141 gaaatcttgg aaggcaaata cgatgatctt cctgaagaag ctttccgaag tgttggccga
    70201 atcgaagacg tcgtagaaaa agcgaaaaca ctaggctact agaaaggggt gccccatgga
    70261 tcaatattta acagtaaatg tggtaactcc tgccggtttg gtctatgatc accatgcagc
    70321 tatcgtcgtt gcacgtacga cagctggtga gcttgggatt ttgcctaaac atgcaccaat
    70381 catcgttcca ttgacgattg atgaagttcg tgtaaaacga acagattcag atacccacgt
    70441 cgattggatc gctgttaatg gcggaatcat ggaagttcgt gataatgttg tttcaatcgt
    70501 tgctgatagt gcggaacgtg aacgtgatat cgatgtatct cgtgcggaac gtgcaaaaca
    70561 acgcgcagaa cgccaaatcg aagaagcaaa agcaaaacaa gatacgaacg aattgaaacg
    70621 ggcaactgtt gcgttgcacc gagcaatcaa tcgtatcaaa gtatctaagc actcataaaa
    70681 tgagatgcgg atgtttatcc gtgtttaaga acagtaaaaa gaggttatga cagaagtggt
    70741 cagcttcaag acataagaag gaatttacga aaattgcttc tcaaattttt gtgaaatctc
    70801 agcttatttt tcgaagaagc tacttctgga acaccgtata tcggaggttg tgaaatcagc
    70861 gttttgtcct gagtattaga gaaacaaatg gaaaatagtt cctcatattt tgccgttttt
    70921 tgagctaata ccgagggtca gatgattgaa caccgtttat ttggaataag ggactgcgac
    70981 aagacttt
//
LOCUS       DACEUF010000004        63426 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.004, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000004 DACEUF010000000
VERSION     DACEUF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.004"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            229..1092
                     /locus_tag="IXG20_000288"
     CDS             229..1092
                     /locus_tag="IXG20_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1608507.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1221..2843)
                     /locus_tag="IXG20_000289"
     CDS             complement(1221..2843)
                     /locus_tag="IXG20_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1608508.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3092..3613
                     /locus_tag="IXG20_000290"
     CDS             3092..3613
                     /locus_tag="IXG20_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608509.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3799..5529)
                     /gene="spxB"
                     /locus_tag="IXG20_000291"
     CDS             complement(3799..5529)
                     /gene="spxB"
                     /locus_tag="IXG20_000291"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ1608510.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5694..6512
                     /locus_tag="IXG20_000292"
     CDS             5694..6512
                     /locus_tag="IXG20_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ1608511.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6615..6812)
                     /locus_tag="IXG20_000293"
     CDS             complement(6615..6812)
                     /locus_tag="IXG20_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608512.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6981..7559
                     /locus_tag="IXG20_000294"
     CDS             6981..7559
                     /locus_tag="IXG20_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1608513.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8604..9107)
                     /locus_tag="IXG20_000295"
     CDS             complement(8604..9107)
                     /locus_tag="IXG20_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1608514.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9465..10490
                     /locus_tag="IXG20_000296"
     CDS             9465..10490
                     /locus_tag="IXG20_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1608515.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10813..11691
                     /locus_tag="IXG20_000297"
     CDS             10813..11691
                     /locus_tag="IXG20_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608516.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12167..12283
                     /locus_tag="IXG20_000298"
     CDS             12167..12283
                     /locus_tag="IXG20_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1608517.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13211..13549
                     /locus_tag="IXG20_000299"
     CDS             13211..13549
                     /locus_tag="IXG20_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608518.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13643..13873
                     /locus_tag="IXG20_000300"
     CDS             13643..13873
                     /locus_tag="IXG20_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608519.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14273..14644
                     /locus_tag="IXG20_000301"
     CDS             14273..14644
                     /locus_tag="IXG20_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1608520.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14918..15610)
                     /locus_tag="IXG20_000302"
     CDS             complement(14918..15610)
                     /locus_tag="IXG20_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1608521.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15613..16398)
                     /locus_tag="IXG20_000303"
     CDS             complement(15613..16398)
                     /locus_tag="IXG20_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1608522.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16635..18035
                     /gene="lacG"
                     /locus_tag="IXG20_000304"
     CDS             16635..18035
                     /gene="lacG"
                     /locus_tag="IXG20_000304"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1608523.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18060..18377
                     /locus_tag="IXG20_000305"
     CDS             18060..18377
                     /locus_tag="IXG20_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1608524.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18390..20063
                     /locus_tag="IXG20_000306"
     CDS             18390..20063
                     /locus_tag="IXG20_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1608525.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20077..21708
                     /locus_tag="IXG20_000307"
     CDS             20077..21708
                     /locus_tag="IXG20_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ1608526.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22199..23764)
                     /gene="guaA"
                     /locus_tag="IXG20_000308"
     CDS             complement(22199..23764)
                     /gene="guaA"
                     /locus_tag="IXG20_000308"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1608527.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(23823..24746)
                     /locus_tag="IXG20_000309"
     CDS             complement(23823..24746)
                     /locus_tag="IXG20_000309"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ1608528.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            24942..25547
                     /locus_tag="IXG20_000310"
     CDS             24942..25547
                     /locus_tag="IXG20_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1608529.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            25855..26871
                     /locus_tag="IXG20_000311"
     CDS             25855..26871
                     /locus_tag="IXG20_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1608530.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            27012..28313
                     /locus_tag="IXG20_000312"
     CDS             27012..28313
                     /locus_tag="IXG20_000312"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ1608531.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28359..29021
                     /gene="deoC"
                     /locus_tag="IXG20_000313"
     CDS             28359..29021
                     /gene="deoC"
                     /locus_tag="IXG20_000313"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ1608532.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            29042..29440
                     /locus_tag="IXG20_000314"
     CDS             29042..29440
                     /locus_tag="IXG20_000314"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ1608533.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29506..30588
                     /locus_tag="IXG20_000315"
     CDS             29506..30588
                     /locus_tag="IXG20_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1608534.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            30800..32362
                     /locus_tag="IXG20_000316"
     CDS             30800..32362
                     /locus_tag="IXG20_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1608535.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32355..33488
                     /locus_tag="IXG20_000317"
     CDS             32355..33488
                     /locus_tag="IXG20_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1608536.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33488..34444
                     /locus_tag="IXG20_000318"
     CDS             33488..34444
                     /locus_tag="IXG20_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1608537.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34543..35709
                     /gene="deoB"
                     /locus_tag="IXG20_000319"
     CDS             34543..35709
                     /gene="deoB"
                     /locus_tag="IXG20_000319"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ1608538.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35733..36551
                     /locus_tag="IXG20_000320"
     CDS             35733..36551
                     /locus_tag="IXG20_000320"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1608539.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36568..37275
                     /gene="deoD"
                     /locus_tag="IXG20_000321"
     CDS             36568..37275
                     /gene="deoD"
                     /locus_tag="IXG20_000321"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1608540.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(37573..38259)
                     /gene="gpmA"
                     /locus_tag="IXG20_000322"
     CDS             complement(37573..38259)
                     /gene="gpmA"
                     /locus_tag="IXG20_000322"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1608541.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(38431..39012)
                     /locus_tag="IXG20_000323"
     CDS             complement(38431..39012)
                     /locus_tag="IXG20_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1608542.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(39145..39828)
                     /gene="rpiA"
                     /locus_tag="IXG20_000324"
     CDS             complement(39145..39828)
                     /gene="rpiA"
                     /locus_tag="IXG20_000324"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ1608543.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            40147..40560
                     /gene="rpsL"
                     /locus_tag="IXG20_000325"
     CDS             40147..40560
                     /gene="rpsL"
                     /locus_tag="IXG20_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ1608544.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            40644..41114
                     /gene="rpsG"
                     /locus_tag="IXG20_000326"
     CDS             40644..41114
                     /gene="rpsG"
                     /locus_tag="IXG20_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ1608545.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            41208..43295
                     /gene="fusA"
                     /locus_tag="IXG20_000327"
     CDS             41208..43295
                     /gene="fusA"
                     /locus_tag="IXG20_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1608546.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELNLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            43467..44654
                     /gene="tuf"
                     /locus_tag="IXG20_000328"
     CDS             43467..44654
                     /gene="tuf"
                     /locus_tag="IXG20_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1608547.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            45061..45369
                     /gene="rpsJ"
                     /locus_tag="IXG20_000329"
     CDS             45061..45369
                     /gene="rpsJ"
                     /locus_tag="IXG20_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ1608548.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            45400..46029
                     /gene="rplC"
                     /locus_tag="IXG20_000330"
     CDS             45400..46029
                     /gene="rplC"
                     /locus_tag="IXG20_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ1608549.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            46057..46680
                     /gene="rplD"
                     /locus_tag="IXG20_000331"
     CDS             46057..46680
                     /gene="rplD"
                     /locus_tag="IXG20_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ1608550.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            46680..46970
                     /gene="rplW"
                     /locus_tag="IXG20_000332"
     CDS             46680..46970
                     /gene="rplW"
                     /locus_tag="IXG20_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ1608551.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            47009..47842
                     /gene="rplB"
                     /locus_tag="IXG20_000333"
     CDS             47009..47842
                     /gene="rplB"
                     /locus_tag="IXG20_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ1608552.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            47885..48163
                     /gene="rpsS"
                     /locus_tag="IXG20_000334"
     CDS             47885..48163
                     /gene="rpsS"
                     /locus_tag="IXG20_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ1608553.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            48186..48533
                     /gene="rplV"
                     /locus_tag="IXG20_000335"
     CDS             48186..48533
                     /gene="rplV"
                     /locus_tag="IXG20_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ1608554.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            48547..49203
                     /gene="rpsC"
                     /locus_tag="IXG20_000336"
     CDS             48547..49203
                     /gene="rpsC"
                     /locus_tag="IXG20_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ1608555.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            49206..49640
                     /gene="rplP"
                     /locus_tag="IXG20_000337"
     CDS             49206..49640
                     /gene="rplP"
                     /locus_tag="IXG20_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ1608556.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            49630..49818
                     /gene="rpmC"
                     /locus_tag="IXG20_000338"
     CDS             49630..49818
                     /gene="rpmC"
                     /locus_tag="IXG20_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ1608557.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            49843..50109
                     /gene="rpsQ"
                     /locus_tag="IXG20_000339"
     CDS             49843..50109
                     /gene="rpsQ"
                     /locus_tag="IXG20_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ1608558.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            50167..50535
                     /gene="rplN"
                     /locus_tag="IXG20_000340"
     CDS             50167..50535
                     /gene="rplN"
                     /locus_tag="IXG20_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ1608559.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            50572..50880
                     /gene="rplX"
                     /locus_tag="IXG20_000341"
     CDS             50572..50880
                     /gene="rplX"
                     /locus_tag="IXG20_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ1608560.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            50908..51447
                     /gene="rplE"
                     /locus_tag="IXG20_000342"
     CDS             50908..51447
                     /gene="rplE"
                     /locus_tag="IXG20_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ1608561.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            51466..51651
                     /locus_tag="IXG20_000343"
     CDS             51466..51651
                     /locus_tag="IXG20_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ1608562.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            51687..52085
                     /gene="rpsH"
                     /locus_tag="IXG20_000344"
     CDS             51687..52085
                     /gene="rpsH"
                     /locus_tag="IXG20_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ1608563.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            52117..52653
                     /gene="rplF"
                     /locus_tag="IXG20_000345"
     CDS             52117..52653
                     /gene="rplF"
                     /locus_tag="IXG20_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ1608564.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            52814..53170
                     /gene="rplR"
                     /locus_tag="IXG20_000346"
     CDS             52814..53170
                     /gene="rplR"
                     /locus_tag="IXG20_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ1608565.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            53191..53691
                     /gene="rpsE"
                     /locus_tag="IXG20_000347"
     CDS             53191..53691
                     /gene="rpsE"
                     /locus_tag="IXG20_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ1608566.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            53706..53885
                     /gene="rpmD"
                     /locus_tag="IXG20_000348"
     CDS             53706..53885
                     /gene="rpmD"
                     /locus_tag="IXG20_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ1608567.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            53929..54369
                     /gene="rplO"
                     /locus_tag="IXG20_000349"
     CDS             53929..54369
                     /gene="rplO"
                     /locus_tag="IXG20_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ1608568.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            54369..55664
                     /gene="secY"
                     /locus_tag="IXG20_000350"
     CDS             54369..55664
                     /gene="secY"
                     /locus_tag="IXG20_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ1608569.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            55726..56373
                     /locus_tag="IXG20_000351"
     CDS             55726..56373
                     /locus_tag="IXG20_000351"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ1608570.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            56564..56782
                     /gene="infA"
                     /locus_tag="IXG20_000352"
     CDS             56564..56782
                     /gene="infA"
                     /locus_tag="IXG20_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ1608571.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            56815..56931
                     /gene="rpmJ"
                     /locus_tag="IXG20_000353"
     CDS             56815..56931
                     /gene="rpmJ"
                     /locus_tag="IXG20_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ1608572.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            56949..57314
                     /gene="rpsM"
                     /locus_tag="IXG20_000354"
     CDS             56949..57314
                     /gene="rpsM"
                     /locus_tag="IXG20_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ1608573.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            57342..57731
                     /gene="rpsK"
                     /locus_tag="IXG20_000355"
     CDS             57342..57731
                     /gene="rpsK"
                     /locus_tag="IXG20_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ1608574.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            57811..58749
                     /locus_tag="IXG20_000356"
     CDS             57811..58749
                     /locus_tag="IXG20_000356"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ1608575.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            58777..59157
                     /gene="rplQ"
                     /locus_tag="IXG20_000357"
     CDS             58777..59157
                     /gene="rplQ"
                     /locus_tag="IXG20_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ1608576.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            59429..60268
                     /locus_tag="IXG20_000358"
     CDS             59429..60268
                     /locus_tag="IXG20_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1608577.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            60244..61113
                     /locus_tag="IXG20_000359"
     CDS             60244..61113
                     /locus_tag="IXG20_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1608578.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            61103..61900
                     /locus_tag="IXG20_000360"
     CDS             61103..61900
                     /locus_tag="IXG20_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1608579.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            62103..62849
                     /gene="truA"
                     /locus_tag="IXG20_000361"
     CDS             62103..62849
                     /gene="truA"
                     /locus_tag="IXG20_000361"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1608580.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT        20105 a        11327 c        13509 g        18485 t
ORIGIN      
        1 ttcacggtgt tccagaagta gcttctacgg aaataagccg aaatttcaca aaaatttgag
       61 aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      121 tattttgatt agaacttgcg aaaataaacc ggttaaagtg tgtctagcgc aagagatttg
      181 ctataataga cgataagggt aaaatgtaac gagaaggggt tgaaaaatgt gtatcaaatt
      241 gtaacagatt cttgttgtga tctgccatac catgtattag caaaagaaaa agttgatttt
      301 ttatcaatga atctccaatt gaatgatcaa gagttagtag atgacctcgg aaagacattc
      361 aattatgacc gctacatgaa tcagctgaaa aatggtgcga tgcctacgac atcacaaatc
      421 aatgttggcc gatatttaga atttttccgt tcttatgcgg aaaaacagat tcctgtttta
      481 tatgtggctt tttccagtgg attaagcggc tcttacagta gtgcgcttca agcggttgaa
      541 ttgctgaagg aagaatatga agaccctgag atctatgttt ttgatacaaa agctgcaagt
      601 attggggaag ggcttttagt attggaagct gtaagattga aaaaagaagg taaaagcatc
      661 gaagaaaccc tagagtggct ggaaagtaat tatttgaaag tccattcatg ggtaacagta
      721 gacgatctga aatatctaga acgaggcggt aggatttcca aagcaagtgc tactcttggc
      781 agtctattga gtgtaaagcc tattattacg gtggataaag aaggaaggct gacgaatgtt
      841 gacaaagttc gaggaagaaa caagtcgatc cagacaatcg tcaatgagac ggtaaaaaca
      901 atcgttgcac cacttgatca aatcgtttat gtggcttacg ctggggatct agagagcgcg
      961 gagaaggcaa aacggctttt ggaagagcag ataaagatga aagatgtgaa gctttatccc
     1021 ttaggaccaa cgatcgccag tcacacaggc tatggctgta tcgcgatttt ttcgatggga
     1081 atcagtcgat aaaagcagta actcattctg taaaaaagcc cagaaattga ttgtaacaaa
     1141 aacagcgact cttaaaagat agatgagcat cctatctctt agaggtcgct gtttttttat
     1201 tttttaagct aaataggcca ttatgcccat aactcgttca attgtttttg aatatctgca
     1261 ttttccaaaa attcatcata tgtcgtatca gctcgatcga tcacaccttt atccgaaacg
     1321 gcaatgatgc ggtttgccaa tgtttggata aattgatggt catgtgacgc aaataagatt
     1381 gagccagtaa atgccatcaa gccgtcattc aatgctgtga ttgattctaa gtctaaatgg
     1441 ttcgttggat catctaatac aagtacgtta gctttcgaaa gcatcatttt tgaaagcatg
     1501 caacggactt tctctccacc agataaaaca ttcactggtt tcaatacttc ttcaccagaa
     1561 aacaacattc ttcctaagaa gcttcttaag aaggtgttat catcttcttc tttacttgca
     1621 tattgacgca accagtctaa aatcgttaga tcagtatcga attctttcgt tgtatccttt
     1681 ggtaaataag aattacttgt tgtcacaccc caacgtactg ttccagtatc tggcgtaatt
     1741 tcacccatga taactttgaa tagcgttgtt gttgttatat cattttctgc gacgaaagcg
     1801 accttgtctt ctttgctcaa attaaaagat atatcatcta aaattttctt tccatcaatc
     1861 gttacactta cattttctac ctgcaataag tcgtttccaa tctcacgttc aggtttgaat
     1921 ccaacgaatg gataacgtct agaagaaggt tggatatcgt ctaaagtaat tttttcaagc
     1981 atttttttac gtgaagttgc ttgttttgat tttgatgcat tcgcactaaa gcgggcaata
     2041 aattcttgga gttctttgat ttgttcttct tttttggcat ttgcgtttgc ttgaagttta
     2101 gccgctagtt gactagattc caaccaaaaa tcataatttc ctacataaag tttgatttta
     2161 ccaaaatcta gatccgccat atgtgtacat actttgttca aaaagtgacg gtcatgggat
     2221 acaacgatca cagtattttc aaaattgatc aaaaattctt ccagccaatt gatcgattgg
     2281 atatccagac cgtttgttgg ttcgtctagc aaaagaacat caggtttacc aaatagtgtt
     2341 tgtgctaata acacttttac cttttgtcca ccagttaatt cactcatttg ctgatggtgc
     2401 aattcttcag ggatgttcag accttgcagt aaaacagctg cttctggttc agcttcccat
     2461 ccattaagct cagcaaattc tccttctaat tcagctgctc gaataccatc ttcgtcagtg
     2521 aaatcctctt tcatgtaaat cgcatctttt tctttcagca cttcatacag acgcttgtgt
     2581 cccatgatga ctgtttcaag tacagtatag tcttcatagt cgtaatggtt ctgtttcaat
     2641 acggccatcc gttcatctgg tcccatagta acggaacctg tggaaggctg cagatcccct
     2701 gataacactt taaggaacgt tgatttacct gcaccatttg caccaatcaa gccatagcag
     2761 tttccggggg taaatttaat attcacatca tcaaatagtt tgcggtctga aaagtgcaaa
     2821 ctcacatcat ttacagtaat caattttttt cctcactttt atcattatta gttttgacaa
     2881 gtcttataga attatatagg ggaaaggtaa aagattcaag gcaatcgaac attctttgtg
     2941 tttcacgtga aacagaatga aaaaaataaa aataaaagcg gagcgaaaat agctactata
     3001 ggatttatta gtttgcaaca gcataatttt tgacgttatt tataagaggg agtactataa
     3061 aactgtaaaa gaaatgaaag aaggattgaa aatggatgaa aaaagaacca tcgactgggg
     3121 ctcattgatt ttgggaattt tatttgtact tgtctcattg ttatcatttc aagaccctgt
     3181 cggaaattta gttgcaattg tagtagtctt tgcgattttt gcctttatta aagggatatt
     3241 tgaactgttt gtacggaaca gattaaaaga gctaacagga tataaaggaa agatgccctt
     3301 aataatcggg atcatcgata ttctagttgg cgtctttttc ttattcaata ttggtgcagg
     3361 agtcgcagca ttgccttttg tttttgcagt atggtttatt gctgattcag ttttagcgtt
     3421 atttactgct gacttggcaa gaggagtaag tgaaggatat tattggttta cgatcatcgt
     3481 caatatttta ggaattttat taggaatttt cttattgttt aatccaattt cttctgcgtt
     3541 gacattaagc tttttagttg gattctattt tatgttattt ggtatcacac atatcgtcta
     3601 tgcatttaga taagtaaaat caaacaaata aaaaagccgg cttccgatta cagttttact
     3661 gttgtaattg gaagccggct tttttatcat ggacaaatat gcacgaggtg atttttaggt
     3721 gtcgagacaa atttatgcaa aaatgtgaga agctatatac acaaatttta tcttaatact
     3781 cgaagcatag cgcagttgtc acaacctctt ttcatataat tcattcaata ctggcaaatc
     3841 atggacttca tatttttctt tgaatgcttg gatttcttct tcagaatatt ttcttggatc
     3901 taaatgcaga tcttctactg gcaatgggcg cttgttattg atcttcacat cgattactac
     3961 aggacgatct gattttgcag ctgcatcaaa ggcaaatcga agttgcgagt attctgtgac
     4021 tgtaaagcta tttgccccga gcgcttctcc aactttaccg aaatctgcat cttctaaaaa
     4081 tacaccaaat ttcttttgtt ccgtatcttc ttgttctgct tcgatgaaac caaaagaatc
     4141 attcgaaaag acaacattaa taattggaag ctgatatttg acttgtgtaa tgatatcttg
     4201 catgaccata gcaaacccgc catcaccact taaggtaaat acttgttttt ctgggtagct
     4261 tagttgtgcg gcaattcctc ctggcacacc attacccata gttgcgaacc atccagatgt
     4321 cgtatgtttt tgtttaccgt tcatattcaa taaacgaata gaatggatag tcgtgttacc
     4381 gacatctgtg acgaaaatcg catcgtcttc agcaatacga ttgatttctt tatagacagc
     4441 ctctggacga atcggttctt cctctcgatc ttcaaaagat ttcaaccaat ttacccattt
     4501 ttctttattt tcttgatttg ctaggtacca gctatcagca gggcgagcat cccctaactc
     4561 gacaagtctt tgcaaagctg tgtttgcatc accgaggatc gacaaagatg tttcatggcg
     4621 tcgtccaaat tttgacgcat cgatatcgat ttggataaat tcagcagttg gattaaaaaa
     4681 tgcacgtcca aatgggaagt cgctaccgac aaataagatc agatcggctt ctgccaatgc
     4741 ttcatttccg ggtttagtag cgacacgtcc agcaaaacca agaaagtttt cataatcatc
     4801 aggaataatt ccttttgcta gaacggaagc gacaacagga actgaaaaat gttctgagaa
     4861 ttctttgatt tgcggaaatc cgtttcgcgt tccttgacca acataaagaa ctggtttttt
     4921 tgctttttca agataaggca aagcagctag caaatctttt tcttctggta atatcacact
     4981 tgttttgtga gtgtgagcgg tggagaaagg ttgttcgtca atttcttcaa aaccaagatc
     5041 aacagggata gtcacgatag ccacgccttt atgctcatag gcggctttaa tggcttcatc
     5101 tactacatgc ggaagacttt gtggtgtcat gactgtacga ttgtatacgc taacatctgc
     5161 gaaaatagga ttttcattta gttcctggaa agagttatag ttcatggatg tagaagcaac
     5221 ttgtcctaag aacgctacta gaggcacatg gtccatctgt gcatcataca aaccattgat
     5281 caaatgtgtc gcaccaggtc ctgcagagcc gaagacagcg ccgactttcc ccgttaattt
     5341 tgcatctgca gcggctgcga gtgcgccgac ttcttcatga cgaacttgaa tgtagttgat
     5401 ttcggttttt tcttgatata gcgcatccat aattgaattg aaagatcctc cgggaatgcc
     5461 gtaaatatgg tcgatacccc agctttcaag aacttttacc attgcgattc ctgcattgat
     5521 ttttgacatg gtgtttcact cctttagcaa tgatgataca taaatttttt gtatctagag
     5581 tatagaactt tttatcgaaa atcccaacta attcgtcttt tcacaaaaat gtagtcatgt
     5641 ttttgggaat atgctagagt agtacataga tagattcaag agaggggaat ggcatggttg
     5701 aatggctatt gagacttgaa ccttggcagc aagcacttgc tgggacagct tttacttatt
     5761 ttatgacagc tcttggtgcc gggttggttt ttttctttaa agaaatcaaa aaagaagtgc
     5821 taaacttgat gttaggattt gcttctggtg tcatgattgc tgcaagtttt tggtctttgt
     5881 tagatccggc tattacaaaa gcagaagaaa atggagatat tgcttggcta gttgtaagta
     5941 ttgggtttgg tttaggcggt gtgtttttat atatggcaga taaaacactg ccccatatgc
     6001 attttggacc acaacatgaa gcagaaggat tgcctaccca tttaaaacgg acgatcttgc
     6061 ttgttttttc tattacattg cataacattc ctgaaggttt agccgtgggg gtagcattcg
     6121 gtgcagcagc aactgcggat aatccgacag ccgctatatt agccgctgtt tcggtggcgc
     6181 taggtattgg tatccaaaac ttcccagaag gagcagctgt atcaatcccg ttgcgacaag
     6241 aaggtctgag tcgtaaaaag gcctttgttt acggacaagc ttctggaatc gttgaaccaa
     6301 ttgctggagt gattggagcg ctactagtaa cgaaagtgga gctgctgctt ccttatgcac
     6361 ttgcttttgc cgcaggagca atgatttatg tcgtggtgga agaattgatc cctgaagcac
     6421 agcagacatt gtccagcaaa cgtcattttg cagtttttgg cgtcatgagt ggatttatca
     6481 ttatgatgat tttggatgtg gcattaggat aaataaaaaa aatatatatg atatatcaaa
     6541 tagggccgcg aacttatatc gcgaccctat ttttttagta agttatatca taatgaagtg
     6601 aatggtatca tacattaata atagtttttt ctatgtgtat ttcctttgat ccagcgaaca
     6661 agccagatga taagtgcgat tgggattaga actttgaccg caaaccataa aagcccgctt
     6721 aggacactga caaaaagact gatgacgaag ctagctgcaa ttagcactaa aagaaccatg
     6781 aagatattta agaacatatt tttttgacgc atacaaaacc ctcctcttgt tcattcttta
     6841 ctatagaaaa agtataacag ggattgttta aaaacacctc caactgaaga ctgattttta
     6901 cttcatctac ttaaatgatc aaacaattgc tttttctctg gtttatcatc taaaatagag
     6961 cggaaaagga ggagaatatc atgcctaaaa atcgtcttga agcatttaca gatgctgtaa
     7021 tcgcgattat catgactatt ttagttttag aacttcatga acctaacgga gacacgtggg
     7081 aggcgctcgc tgcattaggt taccgatttt ttatctatat tgtcagtttt atcattttag
     7141 cgatttattg gaacaatcat catcatttat ttcaagttgt acataagata aatggacggg
     7201 tcttatgggc gaacagtttc tttgtttttg cgctttcgct ttttccgttt gcaacttcat
     7261 ggatcagtga gtacatcaat agcctggctc cagaaatgac ttatggtttt gttaccttga
     7321 ccgctaatat tgcgtattat atcttggcaa aagaacttgt caaagcaaat aaaggccacg
     7381 agaaagcatt gaaggcgatg ttgaataatt acaagaaatc gtatctatcg attggtttga
     7441 atgtgttagg aattgtctta gggtatttag tagcgccagt agccgtgctg atcgcaaaca
     7501 tcgtgattct agctgcttgg atcatcccaa gtcgtaccat cgaatcacaa tatcggtaaa
     7561 atgatccgtg tttaaaattt aagagaaaaa tatagaaaaa ataaataaac gatcaaatat
     7621 ccgagtgata ctagtattca aagagggatg cagatggatc aaaaaagaat tcatgtagga
     7681 ctgattacta gttttgtcac gatgcttatt tatttggatc aaggtcattt gacttggaca
     7741 gtagttggtg tcgtaggatt tttgacatta ttcacagcaa taaaaaaata aacatcttta
     7801 tgtaatctga tgttctatct atttaagaaa agcatgttta cagcagtatg attctgttgt
     7861 aaacgtgttt ttatttgtgg aaaatttaat ttttagaaaa gtgtatcgaa aaatagatag
     7921 ttagtttttc cacagtggat aacttcgtaa gagataaaaa taaaaaatta gtcaaagaag
     7981 ggcgtataga tttcttttga taggagatat acaacagaaa tgaataaata ttaacgtgtt
     8041 ttcaaacagt ataaaacggt gtggtttttg gaataaaaaa ataacatatc tcattttaca
     8101 cttttgttat tttatactta aaaaataacg acacagaaaa taccgtgtct cagatttctc
     8161 tactacttct aaaaactact gtttttttcc tttgaaatgc cttcaaaaag taggatcagg
     8221 cagaatatac tggcagaatc aaggagaagg gagcctaaat aaggagaaaa accatagaaa
     8281 atataaagta attacttata aaacaagctt tttatagaaa aataatatac ctgagtaggg
     8341 tataaattaa gacggaattc ctttctagat tgagatattg actaggaaga atgttaagat
     8401 tttaagcctt taagctaaaa aaaaaccctt tttgttaaat aaggggaata tagcctgtca
     8461 gtttcaaact gcaagtcgtc ctgctgcaat aatatagtag attagagatg aaaatagatt
     8521 tttttaaacc aatgactgaa taaaaagacc gtcatagata cacattggaa tgtgtctatt
     8581 acggtctttt tttacgaacg attttattga agacggacag ttcgggtcaa aaataaaaaa
     8641 gctacgatca gaaataagaa taaaatgtat cccaaaataa tggctagacg gtctaactta
     8701 tttgtcgata gatgtttgtt tagaagttga taggcccata aacctatcag tcctcctgtt
     8761 gtattagtta tcaaatcagt gatgtccgct acgccaatac cgaataggaa ttgaatcaat
     8821 tctgccaaga agcttacaag gaaaataatc cctaattttt tagaaaaaga aagtctcttg
     8881 aaattgacat tcaaaagcac cccaaaagga aaaaagacaa tcaagttata agtggtctca
     8941 cgtaataccg tcgtattttc aaaagaaaaa gtagaaaaag ggacgagatt gacattgctg
     9001 taagaataag tgagaaattg tggaagatta aatgatagct tgaataaaat caaccataat
     9061 agaaagaata aataaatccc taataaaaat tttgatacat accgcataga catctcctcc
     9121 atttaaaaag tataccatcg aaaaccgctg aaaaatcata aaaaagtatg aagtttaagg
     9181 accaacgtag ttatcgtacg aaaattagaa gctttttttg ttgggaatcc ggtttcttta
     9241 acaaagctaa gatagcgagt tgcttggaaa agttgctatc ttttttctgt caaaacgact
     9301 cggaatgttc gttttttgtt ctaatttctc caagttgttt actttttgat actaagttta
     9361 tgaaaaatca agaaatagtt gttatactag gtaatgctga caaacgggat tggcatttca
     9421 tcttggttca gctttttttc caattactag gaaggaggga tagattgttc aagacttatt
     9481 ttcgtttcaa taaaatacaa gataatcctt ttcgtatcat gaatctgatg ctacttatct
     9541 ttattgtatt atggttaggt tacataaatc atgatttttt gatttcaagt ttttcatcat
     9601 tagggatctt tacttttttc tattatcaaa acataccgat gaaatatttg ctgaaacgta
     9661 tgtttctgat cggttgcgga ctgttggttg ctttcgtttt ggggatctta tcgacttatg
     9721 ttttatggct tgaaccattt gccgtagctt tggttgcttt ttccagcaga ttcgtgttgc
     9781 gattattcca tatttctaaa ccaggtggct tgttttttgc gatgctttct gccatgggaa
     9841 cgagcatgca gctgccaatc gctcaattac cgatcgtctc actttatttt tttatgggtg
     9901 tcgtttttgc cctgattgcg gcagtgatta caaaattact ggattcacgg cctgagcaga
     9961 caattgaaaa agcaacttta aaagaaagat ttcacgaaga gcctttagtg atcattgatt
    10021 ctgttttcta cagcgctgcc ttatttcttt ccgtttatgt cagtcacggc ttgaatctgc
    10081 ataatcctta ttggttgacc ttgtcatgtg catcgatttt gctagcagaa aacttggatg
    10141 cgatgaagca tcgacaagtc caatatctga ttggatcgat gggtgggtta tgtgtgtcgg
    10201 cattcttgtc atttgtgccg tttacgcagt tgcagaccat ttttctgatc acttttctat
    10261 atggaatcgc acaatttcta gttgctagaa attatgcagt tgctaatatt tttcttaatc
    10321 cgatggcgtt gatgctatct acacttattc gtggagcata cttgatttct ttgatcgaat
    10381 atcgtttttt agggatcgtc ataggaagtt tcatcggttt aggtgttgcg tgggtaatga
    10441 cggttggttt gcaacattat ttaacggttg ttcgtcaaaa attagaatag aaggacaaat
    10501 atatgtgcca aaagaggtta tgacaagatt tttgtcacaa cctctaattc aaggtgttca
    10561 aaagccagat tctcagcatt aagtcaaatt cacacaaaaa tgtggaaagg aactttcaaa
    10621 aaaattttat atttattcta tcaaaaaatt ttgtttatga tggtaactga ataaactttc
    10681 ctgagatggt caatgctttc cttgaaagtc ttacagatat tctttcttca tagaaagttt
    10741 aagttttata gttagtgtac aataaaagta tgtttatacc atatggtgtc cggataatca
    10801 gtcatttaaa caatgaggtt caaagatatg aaaaggggaa atttgatgaa gaaaataagc
    10861 aatgaaatcc aaattctggc agaaacttta actcttccca aagtcgttat agagcaaatg
    10921 attgtacaag caaaaaacat ccttgatgag gtgtttgagc aatatagtca tgaactaatg
    10981 gatcctaata aagcaaaggc gactgcaaag aaaatagaca aagtctatga agaggaacct
    11041 tttgcagcat tagctattta tctatatgca gcttctcaaa gttggaaaaa aatttatcaa
    11101 ccattgaaga ttccaagaac tatctatttg gcaacaatga atgcttttac tagatttatt
    11161 catgaacatt tccaagtaac aggaagctat cgctttgacc gtggtttttg gatttggcat
    11221 tacactagtg gcttgatctt tcggattggt gaattggagt acgaaatgac atattttccg
    11281 aaagggtaca cggatctaag attagaagga aagcagtgcc tttctcttca tattccttcc
    11341 gatgcagatt tatctcagaa taagataaga gatagttacg aaagtgccat tcgttttttt
    11401 gaactttatt ttcctgatta ccattaccaa gtgatgtaca cagatacttg gttactatca
    11461 cccaatctta ctaaatggtt gaaaaaagag tcaaaaatct gtctttttgc agccgattat
    11521 cgattattat cagttgatga acaggacgat agtggagtcc cttggatctt tggacgagta
    11581 gatgcccaaa tacatgatta tccagaaagt acgagtttac agcgacaagc aaaagagcaa
    11641 ttgcttgcag gcgagcacat tggctctggc ttaggtattc ttacttcata agtttagtac
    11701 ttcgaaatag tttaaaagag cgtgagacaa aagtaaagaa cacttttgtc tcacgctcta
    11761 aacacgtata aacggcggaa gcagaagcaa ctccttcgga aataagctga aattcacaaa
    11821 aatttgaaaa gcaattttcg tgaatttctt cttatttctt ggagttaaac gcttctgtcc
    11881 cagcctcttt tttagtgttc tatcgaacac acagtacgtt caataggtaa actagctata
    11941 acaatagata ttaaagttaa cggacaagag ggcctttttt aggtagctaa aacgtaataa
    12001 tgaaaattta tttccagaga taggagtcaa tcagctagtc tgatcgactg ttttaacaag
    12061 ttgactttca ttttaacttt tgataccatt taaaacacaa atcgtaattg tttcgatttg
    12121 tgttttttag catgactaca atcaaagaag aaagtgggtc gtccatatgg aaaaaacaga
    12181 tatctcaagt gcataccgcc gcttgaaaag cccaaatatc aaaacacgaa aacgtgcgct
    12241 gaagatcatc aaagatgtga aacggaatag tgggaaaagg taagaagaag gaaacttaat
    12301 taaaaaaata gccgaaaaac gtttttcggc tattttcaat aatcggtttt tgtgtaaggt
    12361 ttccgaaaga actgctgttg attttccatt tttaacatat gataaattgg tagaatcttt
    12421 cttttcaaat tgataccatt ccctaattcc agttatatag ttaactacag aaccacaaag
    12481 cacgtacgtt tataaaagta ttatctgtag ttatttttca attccctttc aacctcttat
    12541 tttgaccagt agtattgatc actttacctt ttaacacctt tgttcgtttt tgaaacattt
    12601 ttagtcaaga atggaaacgt aactacataa tcaaaggttt tgcgaaatat ttcatgaaat
    12661 atttcatgta gaagcgagca atacggttgc aaataaccat tatcgtttaa taagcagtta
    12721 cgaaagctcc caatatctct aacgttcagc tcactccatt tcttttcttc aaaaaaatca
    12781 gtgattcgta tctctttcta tttgtcgaaa aagttaactt taaaaagtaa cgccgaaaaa
    12841 atatgcaaaa aaataaaaaa gcagttacta ttgctagaat aacatcaagg aataataagt
    12901 tttggttttc agataatagt cgataagttg tgtattggaa ttttagatac agtaataaag
    12961 tatttttcat gaaattttgt gagaatcact attacaagta tctttaataa aattaatggt
    13021 taaggaggaa cttaaagtag aatgtaaatg aatagttgta gtcagtcgat tgtgatacta
    13081 gactcaaagt tgcaggaata agtttttcaa tttgtgattg gaagataaaa aatgaaaaag
    13141 aagggctttt ttttatactt tgttgattgt agtgtttatt ttccctctgt ttagccaagt
    13201 aacattttct atgactcaac cagtttcaag tgaggaacat gataaccttt ttcgtgctac
    13261 agaagagttt aagaaatatc aggaaaatat tgttccggag actccattag ctagtgagcc
    13321 aattactgat attgatggaa accaaatagg atggtatata cagtacgaag ttatggtggt
    13381 aaataatgac tcaatagtaa aaaatacagt aggcttagag ctaacaccga ttgaaaaggt
    13441 gagttcattg ttaacgtata tttatactga tgaaagtgac gagactgaga tcattttaac
    13501 ggattactca aaaattgcta ctgataaaaa gtatatattg tggatgtaga cactagtgaa
    13561 atactttatg atatagaaat tgatgaaaat ttactagatg tagcacaaga aatcgatcat
    13621 caaaagcagg aagctattcg atatgctggt agaaaaggga aaagaaaata tttattacgt
    13681 gaatgtagca aaagttagag aagatgaaaa tgagtggaaa gaattcaaga gtcgctactc
    13741 aattaactcg actccgacat ttacagttta cagagaagga tcgatagaaa aaactgtttt
    13801 ttggactaaa gaaagtggaa tgtctttagc tgaagtagaa gagtttttgg attatgttag
    13861 tatgcaacag taagggagtt cttattatga aaaattttga cacatattat gttattgttt
    13921 tttcgatatc tttgctgtta atattactaa ctttaatgca gtataaagag tatccagagt
    13981 attttatttt aacttttgta tatttagtaa gtagctacac acttctatat aaattcagaa
    14041 gttcttctag agtttctaaa gtttgtttct taattagctt attgattcta taatattttt
    14101 gatttatata ggaaattaaa aatattatag aatcaacact cagagaacgg gttggttttt
    14161 aatagattta ataagtactg ataatgaaat ttctcctggt tgggaaaacg aagttggtta
    14221 ccctgtagaa gaattttttc aagattactt tgcggagtat gccgatagga taatgagaga
    14281 ttacgtaaca gataaaactc aagaagtcat caatcaatcc cagtcattaa atgaattatc
    14341 tgataaggcg aaagaagaag cagttgccat tatttcttca gggattagtg actatattga
    14401 tatgagtggt gaaatcaatt atggcgataa agacaaaatt gtacgcatga taaaagcttt
    14461 tatcaaaact tttgatgtga aggcagatct taagtttaat gatacttacc tagtaggaaa
    14521 attaattaat atccttggga cagagaaaga tacggaaacg tttatttcct ctttgtctat
    14581 gttgtttaca gataagcatt tttcaactca gcttggtgga gaggaactag tggaaatctt
    14641 ttaaccatta cgcccagcat taataaaagt atatcaaaaa agtactcctg atgattttta
    14701 agattggttt gagaaataat gtgaaaacac tatttttaat gttaattata gatagagatg
    14761 aacgcgtcat atggttaaaa gaatgaaaaa gtcaaagata cactgctata tttcactaag
    14821 gatatagcag tgtatctttc atgtcgtttc actggcagtt ttttataaaa ggaggtgtgc
    14881 atttcagttg cttttctttg gtagttagtt gaatcgttta agaattaacg gatgtggcag
    14941 acaggtgctc tgtccaaaaa ccagtaaaat ctttgatggt tccatcttta tttagctgtg
    15001 cctgttcaaa tactgttttg tttttaccga atggatcaat tgcaataatt gttccgatcc
    15061 ccgcacgttt ggcagcaagt aatcctgagt aagcatcttc gataacaaga caatcagccg
    15121 gagctaagct cagtttgtct gccgctttaa gaaaaatatc cggttgcggt ttcccaggga
    15181 aagagccgtc gtcatagaca atcttgtccc agtcaaacca gcgggctaaa tcgaaaatat
    15241 caaaataaaa agcaacattc tctttgacag tagccgtggc aatcgttaat ggaatctgtt
    15301 ggtcagtcag ctggtcaaga gtcgcttcaa gcccttttgt caactgaagc tcgttttcat
    15361 tttgcaagca aagcatacga taatagcttt ctttttcttc tgacaatacg gcgatctcag
    15421 aggcggtcaa atgctctgaa atgaaatgtt tcaaaatctc cgtattcgtc cgaccatgaa
    15481 tattttttaa aatatcctcg tctgataaac catcttttgt atgatcatgt atcatatgca
    15541 gccaagcagc ttcatgcaga tgagagtcca aaaacattgt tccgttgaaa tcaaatataa
    15601 tccctttcat agttacgctc ctatagtggt attttcggtt gaatcagcaa tttgcagatc
    15661 gacatattgt gcatagtgct cttgaacctc ccgcgagatc tggcgatccg taatgagcag
    15721 atcgaaatca tgcaaattat aaaacacata aaaatcatct cgattgaatt ttttattatc
    15781 aacgagtaga tatttcttac gggaattatt tagagcgatc tgctgtgctt ctccttcttc
    15841 ggtactgtaa gtagagatcg cttcgttgtg aacgccattt gaactgataa atgcttttgt
    15901 aaactttagt cttcttaaat tgtcgttggt gatcggtccg acaaatgcgc cggtattttt
    15961 ccggaaatca ccgccaatca gtagaatttc tggtccatct gcttgagcaa gaatatcaaa
    16021 aactggataa ttatttgtaa tgatccgaat tttcttgttc agtaaatgcg tcgccaatag
    16081 ttcgattgtt gtacctggtc ctaagaaaat cgtttctccc tcgtgaatca aggaggctgc
    16141 tgtggcagca atttcttttt tttcttgaat ctgcagagtg gctttttcag tgtgagaaag
    16201 ttcttgatct aaagtgtagg aaagactttg agcccctcca tggacacgca gcagcttgcc
    16261 cgatttttct aattcatcta aatctctgcg aacggtcata tcagaaacat ctaattgatt
    16321 gatgatttca ttaacggtaa ggattccttt tttattgacc atttctacga tagtaagcaa
    16381 tctttctttt ttcaacattg tcttcacctc taatttaagg ataatctatc atataaacgg
    16441 acaacagtca atctaattcg aacaaaagta aacatttagg gtggttttta tgtttgttac
    16501 aatagatttt attgctgttt tctggttttt atgattattt gtgtttgttt ttgttgacaa
    16561 atgtttcgga tgcgtttaca atgcaatcag ttagaaagag cgaaaactga ttatagattt
    16621 gaggagagat gtacatgctt aaattaccag aagattttat ttttggcgga gccacagcag
    16681 cctatcaggt tgaaggagcc actaaggaag gtggcaaagg cgctgttgct tgggatgact
    16741 ttttagagga acaagggcga ttcagtcccg atccggcaag tgatttttat catcaatatg
    16801 caaaggacat tgaattgtgt gaacgttttg gtgtaaatgg tctaagatta tccattgctt
    16861 ggagccgcat ttttcctgat ggagctggga aaccaaatcc agaaggaatc gctttttatc
    16921 atcgtgtgtt cgaagaatgt aagaaaagaa acgtaacgcc gtttgtgacg ttgcatcatt
    16981 ttgatacccc taaacgtttg tttgaccatg gagatttttt gaatcgagaa acaatagaag
    17041 catttgtttc ctatgccatt ttttgttttc atgaattcaa agaagtcaag gtgtggagta
    17101 cctttaatga aatctatcca gttgcaacca atcaatattt gttaggtgtt ttcccaccag
    17161 gaatcaaata tgattttaca aagatcgtcg cctgtcttca taatatgatg gttgctcatg
    17221 cccgtgtagt gaactatttc aaagaaaacg aacttccggg tgaaattggt gtagttcact
    17281 cattagagac gaaatatgcg gcaacagacg cgccggaaga taagcatgct gcctttttag
    17341 acgatgcatt gtcgatccgt ttcttattag atgctaccta tttaggctat tattcaactg
    17401 aaaccttgac tgctcttgat gagatatgtg aagcaaatca ggccagctat cattttcctg
    17461 aagaagattt cgttgaattg aaaaaagcat ccacgcgtaa tgactactta gggatcaatc
    17521 actaccaatg ccattttgtc aaagcttacg atggagaaaa tgcaatccat cataacggta
    17581 cgggtgaaaa agggacctct gtttataaag tgaagggaat cggagaacgt atttataaag
    17641 aaggaattcc tcgaactgat tgggactggc tgatttatcc agaaggtcta tatgacttgt
    17701 tgctgcgaat taaaagcgat tacccacatt acaacaaaat ctatatcact gagaacggga
    17761 tggggtataa agatcagttt gaagatggca ttattatgga tcagccgaga atcgactatt
    17821 tgagagtcta tttggaatcc ttaagcaagg cgataacagc cggggtaaat gtgaaggggt
    17881 attttttgtg gtcgcttatg gatcttttct catggacaaa tgggtataac aaaaggtatg
    17941 gcttgtttta tgttgatttt gaaacccaga agcgttaccc aaaagaatca gcttactggt
    18001 acaagttggt cagtgaaacc aaaacgatta tttaataagt gataatggag gaagaaaaga
    18061 tgaataaaga ggaattacaa atgcttggtt ttgagatcgt tgcatattca ggcgacgctc
    18121 gcagtacatt attgaaatta ttgaaagaag taagacaagg aaactttgaa catgttgatt
    18181 cggcgttgaa agaagccgac gaaaatttaa cccttgcaca taattcgcaa acaaaaattt
    18241 tagcggaaga agcatctggg aaagagatgg agatgggatt catttttatc catgggcagg
    18301 atcatttaat gaccacttta ttgctgagag atctgattca agactttatt gtactgtaca
    18361 gacagaatca aggataggag aagaacgcta tggatggaat cattaaacaa atcgaaaaag
    18421 ggaaaccatt ttttgaaaaa atctcacgta atagttattt aggagccgtt cgtgacggat
    18481 ttttaacagc gatgccagct attttatttt caagcatttt cattttgatt gctgcggttc
    18541 ctgagatttt tggttaccaa tggcctgaat ctgtctccac atggctatgg aaagtctata
    18601 attattcgat gggtgttgta gggttactcg tttcagcaac cactgctcgc tgcttggctg
    18661 attcgatgaa tcgtcgctta ccgaaaaaca aagtaatcaa caccacctct gtgatgctgg
    18721 cctctgtggt aagtttcctg ttgttaagcg tctctccgat tgaaggtgga ttttcaacgg
    18781 aatatatggg gacaaaggga ctactggctt cctttgtcgc tgcattttta acagttaatt
    18841 tatataagtt ttgtgtgctg aaagatatta ccattaaaat gcctaaagaa gttcctggaa
    18901 cgatttcgca aaccttccga gatatttttc ctttctcttt ttcagtattt gcggccgttt
    18961 taattgattt aggcattcga caaatcttcc aaatctcatt tgcagaagca atcattacgg
    19021 tgctgcagcc gctatttaca gcagctgacg ggtacttagg aatcgctatt atctggggag
    19081 cgatggcgct gttttggttt gtcggtgttc atggaccgtc gatcgtggag ccggcaattg
    19141 cggcaattat ctatgccaat gtggaaacca atctgcaatt gttcaattcc ggtgaacaag
    19201 cttctaaagt actgacggtg ggtttaggga attttgtagg aactatggga ggaactgggg
    19261 caacattggt tgttcctttt atcttcctac tctttgctaa gtctaagcag ttgaaggcag
    19321 ttgggaaagc ttcctttatt ccagtaagtt tcgctgtcaa cgaaccattg ctatttgcgg
    19381 cacccatgat tttgaaccct tattttttta ttccattttt gttaacacct gttgtaaacg
    19441 tttgcctgtt caagtttttt gtcgatgtat tgggcatgaa cagctttatg tacgttttgc
    19501 cttgggcaac tccagcaccg atcggtttgg ttctaggaac gggcatgggt atcttagctt
    19561 ttgttctagc agcggttcta atcgtcgttg acttcctgat ctatctacca ttttgcaaag
    19621 catacgatgc tgtcttagta gagaaagaag cacaaaaagc agaggtgatc gcaaccgagg
    19681 aaggtgcttc tgccgatgta cgtgaggaag tacctgtggc agcgagtcaa gttatcagcg
    19741 atgcaaataa agaaatcaat gtgttggtac tttgtgctgg cgcagggaca agtgcgatgt
    19801 tagctaatgc tttaaaaaaa ggtgcatctg aatcgaaagc accgatcagt gcaatggcgg
    19861 gtgcttatgg ttctcattac gaaatgctgc aggattttga tatggtggtc ttagcaccgc
    19921 aagtcaattc ttactatgat gatatcaaag aagacaccga tcgcttagga atcaagctgg
    19981 ttgcgacaaa aggagcagaa tatatcggat taacgagaga tcctgaaaaa gctgttcagt
    20041 ttattctgaa ccaatttcaa taaaggagga gccataatga aaatgggaat ttggttgcgt
    20101 aaagctgttt tatttgcagc tgtgttaggg gctttggggg ctgtttcaaa aactgttgaa
    20161 gcatctgcca gcgaatttat tcgcggagca gatatttcga tcctgtcaga tatggaaaaa
    20221 tctggtgcaa aatattatga agacgatatt tcaaaagatg ccttagagat tttaaaaaat
    20281 aatggtgtca actatgttcg actgcgtttg tggcaggatc cttataatag taatggagaa
    20341 agttatggag cagggacaaa tgatctggaa acgacgattg accttgcaaa acgagcaaaa
    20401 aatctgggac ttaaggtgct gctggatttt cactacagtg atttttgggt tgatcctggg
    20461 aaacagaatc ttcctaaagc atggcaaggt ctgacctttg aagaaatgaa tacggcattg
    20521 tacgactata caaagaacgt tctgtcggaa atgaagcagc tggacgttta tcctgatatg
    20581 gttcagatcg gcaatgaact gaatagcgga atgctctggc cttatggcaa gtcttgggga
    20641 gaaggcgggg gagaatttga tcgattggct gcttttttga agtctggtat ccaagctgtt
    20701 cgagacactc aacctaaaac aactccagta atgctccact tagctgatgg tggcgataca
    20761 ggagcattta cttggtggtt tgatgaaatc acgagtcgag gtgtgtcatt tgatttaatt
    20821 ggagtgtcgt attatccgta ttggcacggt tcaatgaatg aacttcaaac caatatgaat
    20881 gcgatcagcc aacggtatgg taaagaagtg atcgtagttg aaacagctta tgggcataca
    20941 acagcgaatg ccgatactat gccaaatgct tttggtgaag ccgaagcagc tgcgggggga
    21001 tatgaaccaa caccgacagg acaagcagag tatttgctgg atcttgccga tcggattcag
    21061 gcagtaccga ataatcgcgg ggcgggcttc ttctattggg aacctctttg gtacaacgga
    21121 aatgtgagtt gggcaacaca agcaggtatg aattatctca gtgttcaatc cactatggga
    21181 aacgaatggg acaaccaagc gatgtttgat tttgctggaa acgccttgcc aagcttgcgt
    21241 gcgtttaaac aagcaggggc gcaaataaat ctagttaaaa atgcaagttt cgaaagcgat
    21301 ggaccaacga ccagtccttc caattggcaa acctggtttc aacaaggaac cgctaagggt
    21361 acgatcaaaa cggaagcggg cacttttttt ggggactaca aactaacttt ttggcaggat
    21421 caagcatacg aggcatcagc ttatcaaaat gttagtggca taagcaaagg aacttacaca
    21481 ttgagtgcct atgtaatgtc tgggggagag tttgatacta atcagttata tatcaaaaat
    21541 tatggtggag cggaactaaa tactgcggtt gttaaaacaa gtgaatggac aaaaattaca
    21601 atcaaagaca ttccaattac aacaggtgtt tgtgaaattg gggtttatag caaggcttca
    21661 ggaaatgctt ggacgaacgt tgattttgtt tcattggtga aacaatagac tgcggatcgt
    21721 ttcgattcaa gatcggaggt tcccttcagc cacatcgtta gcgtagaagg caggaatcga
    21781 acctgcacct cgcctttgcc aaaaataacc agaaacgatt tccagtcatt tttaccaaaa
    21841 cgagtaaagc accagacttc atctacactt tgtattatgc taaagagagt tcaacgattt
    21901 cgtaagaagc aatgaaaaaa ttgcaaaaag ttgtgcagat aacataaagt ttgtttaaga
    21961 aagggtaacc ttagaattcc gtcgtgaata tgatttgagc acttagatgg atagaaaaaa
    22021 gttctcggaa aacagttcgt ttttcgagaa cttcttttta tgataagaat gaaacaaatt
    22081 aacttatatt atagtctctt ttggtaggga accatcttat ttttaatgaa aatcaagcat
    22141 tttagctatt gaagaatgtt gatttatagg gattccccct ccatattatg ttggctcttt
    22201 attcccactc cactgttgct ggtggtttag aagtgatgtc atacacgata cggttgacgt
    22261 gggctacttc gtttacgata cgtacagaga ttttttgcaa gacttcccat gggatacggg
    22321 caaagtcagc ggtcattccg tcgattgaag ttacagcacg gataccgact gtgtagtcgt
    22381 aagtacgtcc gtcacccata acaccgacag agcggatccc tggaagaaca gtgaaatatt
    22441 gccagatatc acggtcaagt ccagcagctg cgatttcttc acgcaagata gcatcagatt
    22501 cgcgtacgat ttctagcttg tcttcagtga tttcgccaag aacacgaata ccaagaccag
    22561 gaccagggaa tggctgacgc caaacgattg aatcaggcat gcctaactga gtccctaatt
    22621 cacggacttc gtctttgaat aatgtgttta atggttcgat caattcaaat tgcatatctt
    22681 ctggcagtcc acctacatta tggtgagatt tgatcgtctg tgctgtttct gtaccagatt
    22741 cgataacgtc agtatacaac gttccttgtg ctaggaaaga gacgccttct tcaccagcaa
    22801 gttttgttgc ttcgtcatca aaaacatata cgaattcatt cccaatgatt ttacgttttt
    22861 gttctgggtc agatacacct ttcaacttgt ctaagaagcg atctcttgca ttgactttga
    22921 tgatgttcaa gccgaatttt ccgcctagac tttccattac ttgttccgct tcgcctttgc
    22981 gtagcagacc gtggtcaaca aagatagatg tcaattggtc accgatcgct ttttgtaaaa
    23041 gaaccccaac gacactagaa tcaacgccac ctgaaagacc aagcaagact tttttgtcac
    23101 caacttgttc acggattttt tctacttgca tatcgataaa gttatccatt gtccagttac
    23161 cttcacagtg acaaacatca aaggcaaagt ggcgcagcaa ttcgattccg tattcggaat
    23221 gacgtacttc tgcatggaat tggataccat aaaaattacg tgttgtgtct tggatagaag
    23281 caatcggaca atctttgctt gttccgactg tttcaaagcc ttcaggaact ttagttacca
    23341 agtcaccatg gctcatccat actgtttgtt gttttggtgt ttctttaaac aagacagctt
    23401 gatcgcttgt tacttctaac aaggctttcc catattcgcg gtttttagca ggctctacgg
    23461 aaccgccaag tttgtacgtg atcaattgca taccatagca aatccctaat acaggaatac
    23521 ctaaattgaa gatctcagga tcgatcccaa atgcattctc atcatataca ctgtttggtc
    23581 cgccagaaag aatgattccc tttggattca tcgcttttac ttcttctgca gtaatgcggt
    23641 ggctcaataa ttcggaaaag actccaagat cacgaatacg tcttgtgatc aattggttat
    23701 actgactacc gtagtctaat acgataatct tttcaacgtt cgtcatatcg gcaacgtttg
    23761 tcaaatttat tacccctatc cctttaaatt ttatctcaaa atagtcaaac tattttaaaa
    23821 tgctaatatt ttcgcatgaa aatcgaatcg atgatatgat tttcagtttt atggagaatt
    23881 atatcggctc ttcctcgtgt aggcaagata tattcctgta aatttttcaa attcacattt
    23941 ttccatacat cttttgccat tttaaaggct tcttcacgat ctccgatagc aaaacgataa
    24001 taataattct ctggattttg gaaagccgta tccagtaacg caccaaaacg atccaaatac
    24061 catttttcga tcagcacggg atcggcatcg acatagacag aaaaatcaaa aaagtcgctg
    24121 acgtagattt gctgattcgc aggtaattga agcgtattga tcccctcaac gatcaagata
    24181 tcaggctgct ggatcaattc gtacttgtct ggaatgatat catacacatc atgtgagtaa
    24241 acaggtactt ttatctcttc ctgaccagat tttacacggt tcaagaaatc gatcagcatc
    24301 tccatatcat aactttcagg aaatccttta cggtccatga ttccacgctc ttttaatata
    24361 cgatttggat aaagaaatcc gtcagtcgtg atcagctgaa cgtttcttcg tttgaaggta
    24421 cgagacaaaa tcgtctgcag cagtctggct gttgtacttt ttccaacggc tacacttccg
    24481 gcgatcccga taataaaagg cggtacttct acgtatttat gaagaaataa acctttactc
    24541 aagaacaaag attcaaattc tttcatataa agataaatca gatgagcaag aggtacatag
    24601 atatctcgaa catcctgcat ggaaatgcgg tcattcaaac ttttgatatt atctagttca
    24661 tcttgtgtca aaggtgcttg cccattacga taaaattctc gccattcttc tcttgccaca
    24721 cggtagtagt tcattggatc attcatatta gcctcctgcg ttctcccatc aatggaacta
    24781 ttttaacaca aaataaacgc aaaccccacc acttaagaga gattgccgat ctttttgtat
    24841 aatctttata atagaaagaa acggagtctt attcttgact tgcgcacgga tacataaaag
    24901 gatagactgt tgtcatgaaa gaaatgagaa ggatggaaga catgacaaat cattactatt
    24961 ctgaacgccc agatacggcg cacgattttg aacaatgggc ttttgagtta aaaggcagaa
    25021 cctttcaatt tgtaacagat tctggtgtat tttcacgtga aacagtggat tatggttcac
    25081 gtgtcctgat tgatgctttc gattgggaag agttacctga tgaaggaaag atcttagatg
    25141 tgggatgcgg gtatggaccg attgggttag ctttggcatt tgcgacacag cggtttgttg
    25201 aaatggtcga cattaatgaa cgagcggtaa gtctagcgca aggaaacgca aagcgtaacc
    25261 aaatcgaaca tgttgatatc catcaatcga atatctatga agcagttcat gaagaaacct
    25321 atgcagctat cgtaagcaat ccgccgatcc gtgctggaaa aaaagttgta catgaaattt
    25381 tgacaggcgc atatcctcgg ttgaagaagg gcggtacact gacaattgtg atccagaaga
    25441 aacaaggagc gccgagtgcg caaaagaaaa tggaagaaac atttggtaat gcagaaatcg
    25501 tcacaaaaga taaaggatat tatatcatca aaagtgtgaa agaataacta gctgaaaaat
    25561 agtggagcga cgaaagagtg gaactgggcg tgcatgtacg ggtacgtatc agttcttttt
    25621 ttgttgagaa gtttttaaaa acaaagaact attttttctt caaaaagaaa tagtaggaac
    25681 agagtcgttg ttttttacga taaaataaat aagatacaga acttgtatag aaggctgcca
    25741 agtcggtaga taaaggaggt ttttatcaaa aaacatactt tccttttttc tctattcttt
    25801 tcttgggggt tttggtacac tgatagggaa tttagataga agggagccaa taggatgaag
    25861 ctgaccatca gagatatcgc agaaatggca ggcgtatcag taacaactgt ctcacagatt
    25921 ttgaataaca aaggaagcag atttagtgat aagacaagaa aaaaagtgtt gtcgatcgtg
    25981 aatgaacatc attataagcc cgattatttc gcttcaaatg tgatcaatcg acattcaaag
    26041 accatcggaa tgatcgtccc tgatgtcact gatttcttct tctccaaact gatcgaaggt
    26101 gtagagagct atttgaatcc tttgggctat gtgatcatgt tatgcaattc tcgtcatagc
    26161 caagaaaatg agatcaagta tttacaagag ctttcccacc gctcagtgga cggaatcttg
    26221 ctggccacac caaatatttt acctgagaaa tatgcattag acagcggttt ttatcaaaaa
    26281 atgccaatca ttttaatcga tcgcggattg aatcgtcggg ataacggacg gcttatcgta
    26341 aaggaatatg agggcgccta tcaagctgtt tcgctgttta ttgaaaatgg ccatacgaag
    26401 atcggcatgc tgaaggaaac cactggatac tatcaattag aagaacgttt caacggatat
    26461 cgtcatgcgc tgaaagatca tggcctgcct tttaatggga aatttgttga acatggagag
    26521 ttgactgtcc aaggtggata tactgcatca aaaaaattac tcaaacataa agatatcacg
    26581 gctatttttt gcgggaatga tgccatggcg atcggatgct atcaggcgat cgatgaattg
    26641 ggcaaaaaaa tcccagaaga tatctcagtc ataggattcg atggattaaa gctgtcagaa
    26701 tacatgattc cgcaattgac gacagtgcag cagccaagtt ttgatattgg attttatgct
    26761 gcacgtttcc tgatcgatac gatcgaattt cctcaaagaa aagttccgaa caaagtgttc
    26821 gaaacaaaat taattattcg tgagagcgta aaagtattga caaagggcta actcggcggt
    26881 atattgctta tgtaagcatt tacaaaggct agttagtgct ttttttattt ttagtaaacc
    26941 agtttactaa agtggtttac ctttcctaaa aataatgtat aataccgaat gaagacaagg
    27001 aggatcaggc aatgcgaatg gtggatctga ttgaaaaaaa gagggatgga aacgagctgt
    27061 cgaaagagga aatcgagtac atcgtcacga actatacgaa tggaaagatt ccagattatc
    27121 aagtcagtgc attattgatg gcaatttttt atcaagacat gacaaatgaa gaaatcacga
    27181 acttaacatt ggcgattgct aattctggtg atgtcatcga tttgagttct cttgaaggaa
    27241 taaaggtaga taagcattcg acaggtggcg ttggtgacac gaccacattg attttagccc
    27301 ccctagtagc tagtgtcgga gtgacagtgg ctaaaatgtc tggacgcggt ctaggttaca
    27361 caggtggtac actagacaag ctagaagcaa ttcccggatt tcagatcgag ctttcggatg
    27421 aagaatttgt acgcattgtt aatgaaagta aagtagcggt aattggtcaa tcaggaaact
    27481 tggcaccggc ggacaaaaaa ttgtatgcct tgcgtgacgt cacagcgacc gttgattctc
    27541 ttccgctgat tgccagttcg attatgagta aaaaaatcgc ggcaggagca gatgcaatcg
    27601 ttttggatgt gaccactgga gacggtgctt tcatgaagaa catcgaagat gcaagacgtc
    27661 tggcaaagac catgacgagc attgggaaac tggctaaccg agaaacagtg gcggttatct
    27721 cagatatgtc agaacctctt ggcgaagcta tcggaaatag tctagaagtt gtcgaggcaa
    27781 tcgaaacatt acaaggaaat ggtccagaag atctagtaga aatgtgctac gccttaggaa
    27841 gccaaatggt tgttcttgct ggcaaagcta agacaaccga tgaagcaaga acgttgcttc
    27901 aagaagctct ggaatcaggt aaagcgttag caaaattcaa agaaatgatc caaaatcaag
    27961 ggggagaccc agcgatcgtg gaacatccag aacgaatttt gactgcacgc tacacaatgg
    28021 agcttcctgc taaacaaagc ggagttgtgt caaaaatcgt ggcaaatgaa ttaggaattg
    28081 ctgcaatgat gctaggtgct gggcgtaaaa ccaaagaaga agatattgat cacgctgttg
    28141 gattgaaact gcataaaaaa attggtgaca ctgtgacaaa aggtgagtca ctattgacga
    28201 tctatagtaa cgacgaagaa atcacttctg tcattgaatt attatataaa aatattgaaa
    28261 ttggtgaatc ggcaatgaag ccaacgttga ttcatgacat catcacagaa taaaggaaaa
    28321 aagcggactt taccgtttta aagaaaaggg gaaaagaaat ggaactaaac cgaatgatcg
    28381 accatactat tttgaaagca gacgcatcta aagaagctgt gatgcaaatt atcgaagaag
    28441 caaaaaaata ccatttttat tctgtctgca tcaatccagc atgggttgcg ctagcgaaag
    28501 aacaattaca aggtgagcca gtagccgtat gtacagtaat cgggtttcct ctaggagcaa
    28561 acacacccga gacaaaagca tttgaaacaa cagatgccat caataacggt gcagatgaag
    28621 tcgatatggt catcaatatc ggtgctttga aatcaaagga tgatcaacaa gtccaaaaag
    28681 atatcgaagc agttgttgaa gcagcaaaag atcgtgcgct tgtcaaagtt attattgaaa
    28741 catcgttatt agatagagaa gaaatcatcc gagcttgcga aatagcaaaa gctgcaggcg
    28801 ctgattttgt caaaacatca actggtttct caacaggcgg agcaaaggta gaggatgtcc
    28861 gtttgatgcg tgaaactgta ggaccagaaa tgggtgtcaa agcttccggc ggtatccata
    28921 acgaagaaga agcaatggca atgatcgaag ctggtgctac aagaatcgga acaagcgcag
    28981 gagtagcaat cgtatctggt tcaactggtg aaggctatta attttttcaa aggagaagct
    29041 catggcagaa ctcaaacaag aatggatcaa cacagctatt gaagcgttag ataaagccta
    29101 tgttccttat tcccattttc cggtaggcgc ttgtctcgta acggaatcag gcaagattta
    29161 tcaaggcatc aacattgaga atgcatcatt tggattaacg aattgtgccg agcgcacagc
    29221 ttttttcaaa gcagtatctg aaggagaaaa aacatttacg catctagtcg tagcggggca
    29281 cacccccgat cctatctctc cttgtggtgc atgtcgacaa gtaatggcag aattctgttc
    29341 ccctgatatg ccggttacgc tagtaggaga caatggtgtg acgaaggcaa cgaccgttcg
    29401 tgcactgctg ccttatgcat ttactgaaaa agatctataa ctcatgttca ttcagtcagc
    29461 tgactgttga aaaataaaac aattatttta ggaggcttta cagaaatgaa aaaagcaaaa
    29521 ttatttggtt taggtgctgt cgcactagca gcagggttat ttttaggtgc atgcggcaac
    29581 aacggctcta ctgattcatc cggtggcaaa agctcatcag acacaacaac tgcagcattg
    29641 atcacagaca caggtggtgt agacgaccgt tcattcaacc agtcagcttg ggaaggacta
    29701 gaaaaatggg ggaaagatca aggactttca cgaggaaatg atggcttcca atatttccaa
    29761 tcttcaaatg aatcagatta catcccaaat attgaccaag cattgaatgc tggattcaaa
    29821 acgatttttg gtattggtta taaactaaaa ccagcgatcc aagaacaagc aacaaacaat
    29881 acaggtacga actttgttat tatcgatgat gtcatcgatg gattagataa cgttgtatct
    29941 gcaactttca aagacaacga agcttcttat cttgcaggtg ttgcagcagc ttatacgact
    30001 gaaacaaacg tagtcggctt tatcggcggt gtaaaaggtg aagtaatcga tcgttttgat
    30061 gctggattca aagccggtgt tgatgcaggt gcgaaagaat taggaaaaga aatcaaagta
    30121 ttgaatcaat atgctggtga cttctctgct ccagataaag gacgttcaat tgctcaaggt
    30181 atgtatgcac aaaacgcaga tatcatcttc catgcttcag gcggtacagg taacggtgta
    30241 ttccaagaag caaaatcatt gaacgaatca ggcgacaaaa aagtttgggt tatcggtgtt
    30301 gaccgcgacc aatcagacga aggcgaatac acattaaacg gcgaaaagaa aaacttcaca
    30361 ttgacatcta cactgaaagc agttggaaca gtagtagaag atttggcaca aaaatcagcg
    30421 gacggtaaat tccctggcgg cgaacacact gtttatggat tgaaagaaga cggtgttggt
    30481 ctaacagaag gacaactttc tgacgaagcg aaaaaagcag ttgatgaagc aaaagaaaaa
    30541 atcatttcag gtgacgtaaa agttccagaa actcctgaag aaaactaaaa ccatttctat
    30601 agccttgtta gacacatgtt tgacaaggct atggtttgat tagaacaaga attgttcatt
    30661 tatccaagct gccttgtgac agtttggata agggaacaac tccctgaagt aatgtttgtt
    30721 tcaaaagaag aacgctcact aagcgtgaat cttagtcaat cgttgcccta gatagaaatc
    30781 gttaggatgt gaaaaaaagg tggttcaaga aaatattgtc atagaaatgc agaatattac
    30841 caaagaattt ggaacattca aggcaaatga caacatcaat ttacaagtaa aggcaggaga
    30901 aattcatgcg ttgcttggag aaaacggtgc tggcaaatct acattgatga acgtgctttc
    30961 cggattatta gagccgacat cagggaaaat tttgatgcgt gggaaagaag taaagatcac
    31021 aagtccgacc aaagccaacc aattagggat tgggatggtc catcagcact ttatgcttgt
    31081 tgatgccttt actgtaacag aaaacatcgt gttgggaagc gagcctagtc gcgcagggat
    31141 gcttgaccat aaaaaagcac gaaaagagat ccaaaaagtt tctgaacagt atggattgtc
    31201 agtcaaccca gatgcttacg ttcgtgatat ttcagttgga atggaacaac gggtagaaat
    31261 tttaaaaaca ctttaccgag gagcagatgt actgattttt gatgagccga cagctgtatt
    31321 gacccctcaa gaaattgatg aattaatcgt gatcatgaag gaattagtca aagaaggcaa
    31381 gtcgatcatt ttgattacgc ataagttaga tgaaatcaaa gcagtagctg accgctgtac
    31441 agttatccgc cgtggaaaag gaatcggtac agtcaacgtt aaagacgtaa cctcacaaca
    31501 attagccgat atgatggttg gaagaacggt ttcattcaaa acgatgaaaa aagaagcgaa
    31561 gcctcaagaa gtcgttttgt ctattgaaaa tctagtggtg aaagaaaatc gtggattaga
    31621 agccgtgaaa aacttgaact tagaggttcg tgctggcgaa gtacttggta tcgctggaat
    31681 cgatggaaac gggcagtcgg agttgatcca agctttgact ggtttgcgaa aggcagaaag
    31741 cggacatatc aagctaaaag gggaagacat cactaataaa aaaccccgaa aaatcactga
    31801 acatggtgta ggacacgtgc cagaagaccg tcataaatac gggttagtcc tagatatgac
    31861 attatctgaa aatatcgcac tacaaaccta tcatcaaaaa ccttacagta aaaacggcat
    31921 gctgaattat tcagtaatga atgaacatgc cagagaattg atcgaagaat atgatgttcg
    31981 aacaacgaat gaacttgttc ccgcaaaagc tttatcaggc ggaaatcagc aaaaagcaat
    32041 tatcgctcgt gaagtcgacc gagatcctga tctgttgatt gttgcaaatc caactcgtgg
    32101 actggatgta ggtgcgatcg aatttattca taaacgtctg atcgaacaaa gggacaaata
    32161 caaagcagtg ttactgatca gtttcgaatt agaagaaatt ttaaatgttt cggatcgtat
    32221 tgctgttatc catgaaggag aaattgtcgg tatcgttgat ccgaaagaaa catctgaaaa
    32281 tgaattaggt ctattgatgg cgggctatac cttagaagaa gcacgaaaag agttagaaaa
    32341 gggggctggc gcacatgaat gatcgcagcg aaaaattccg taatttatta gtgcctgtac
    32401 tttcagttgt tttaggattg atcttaggcg cgatattgat ggctgctttt ggttttcagc
    32461 ccatccaagg ttattcttct atgctagatg cagcccttgg ttcgcaaaga gggataggtg
    32521 aaacgttaag gcaagcaacg ccgttgattt ttacagcatt aggtttttct gtagctaact
    32581 cagctgggtt tttcaatatt gggctttctg gacaagcact ttgcgggtgg atcgtcagtg
    32641 tctgggtggc acttgcgttt ccagatctgc caaaagttgt tttgcttccg ttatgcgtga
    32701 tcttaggtgc cttagctggt gcgatggcag cggctgttcc aggtttactt cgagcatttt
    32761 ttggaacaag tgaagtgatc gtaacaatca tgatgaacta tatcttatta tacgtaagca
    32821 catttgcttt acatgaagtg atgcctgaaa gctatcgctc aagtttggac tcttccaata
    32881 tgatcagtca aaatgcctca ctgcggttac catggttaac acaagctttt ggcggctctc
    32941 gcgtaaactc aggattattt ttagcaatca tcgcgcttat tttcgtttgg ctgctgatga
    33001 agaaaacgac tttaggattt gaaatccgtt ctgttggatt gaatcctttt gcttctgagt
    33061 atgcaggaat gagtagtaaa cgtacgatcg ttttgtcgat ggtcatttct ggcgctttgg
    33121 cagggcttgg cggtgtggtt gaaggattag gaacgtatca aaacttcttt gttcaaacga
    33181 cttcgctatc tattggattt gacggaatgg ctgtttcctt gcttggttct ggatcagcaa
    33241 tcggtattct tttatcagcg atcttgttca gtattttgaa gatcggtgga cttggcatgc
    33301 agacaggagc aggtgttccg tttgagatcg tcaatgtatc gattgctttg attatcttct
    33361 ttgtcgggat caacttcctg attcgtttcg tcatggctaa agtcttccaa gggaaaaaac
    33421 aagaagagat cgttgcgaca atagaatcga aaccaacgaa tcaaaaccat gaagggggcg
    33481 aactataatg tcgactatcg aattaattgc cttgatcctt acttctacac tggtttattc
    33541 aacaccgcta attctaacgt cactaggcgg aactttttct gaacgaagcg ggatcgtaaa
    33601 cgtagggcta gagggaatca tggtcatggg tgcttttagt tccatcgttt tcaacttgac
    33661 gatgagtcca tcacttggtt ctatgacacc atggatcgct attttagtgg gtggcgtcgt
    33721 aggtatccta ttttctctga ttcacgcagt agcaacagtc agtttgcgtg ctgaccatat
    33781 tatcagcggg acagtaatta atctgatggc acctgcttta ggtgttttct tgatcaaagc
    33841 atggtatggt aaaggtcaaa ctgataacat cacgcagaat ctggggtatt tttctttccc
    33901 aggattagca gatattccgg tcattggacc aattttcttt aaaaatacat tcttacctgc
    33961 ttattgtgct attttgattg ctgtcctagc ttgggtgatc atcttcaaga cgaaattcgg
    34021 cttgcgcctc cgttcagttg gtgaaaatcc tcaagctgcc gacacattag gtatcaatgt
    34081 atatgctatg cgttattcag gcgtactgat ttctggcttg ttaggcggta tgggcggtgc
    34141 tgtttttgct cagtcgattt ccggtaactt ttcagctggt acgatcgttg gacaagggtt
    34201 catctccatg gctgctatga tctttggtaa gtggaatcct ttaggggcaa tgggtgcctc
    34261 attgttcttt ggttttgctc aaagcttgag tatcattggc gcacaattac cagttatttc
    34321 ttcgattccg ccagtattgc tgcaaattgc tccatatttg ctgacaatca ttgtgctggt
    34381 tgttttctta gggaaagctt ctggtccaaa agcaaacggg aagaactata tcaaatcaaa
    34441 ataatctttt agcgaataaa aagggtgggt tacgggaagt gtttttcgtc tcgccctttt
    34501 tgttttaaag gcaataaaaa taaaaagaaa gaaggaataa tcatgtttaa acgcgtacat
    34561 ctgatcgtca tggattcagt aggaattgga gaagcaccag atgccgataa gttcggtgat
    34621 gttggttctg atacattagg acacattgcg aaagaagcag gattgaacgt tccacattta
    34681 gagcagcttg gtttaggaac gatccgtcct cttgaaggtg tggaatcagt gaaagatcat
    34741 caaggctatg cgacgaaatt agaagaagtt tctgtaggga aagatacgat gacaggccac
    34801 tgggaaatca tgggattgaa tatcaaaact ccatttcgtg tgtttcctaa tggattccct
    34861 gatgatctga tcaaacaaat cgaagaattt tctggccgta aagtcgtatg taacaaacct
    34921 tatagcggaa cagctgttat tgacgattac ggtgaacacc aaatgaaaac aggcgattta
    34981 attgtatata cttctgccga tcctgtcctt cagatcgcgg cacatgaaga aatcattcct
    35041 ttagaagaat tatatcggat ttgccaattc gttcgtgata taacaaaaga tgatccgtat
    35101 atgattggcc gaatcattgc acgtccgtac gtgggtgaac caggagactt cacacgaaca
    35161 agcaatcgcc atgattatgc gttgaatcca ttcggacaca cagtccttga ttctttgaaa
    35221 gaagcaggaa aggacgtgat tgctgttgga aagatcaacg atattttcaa cggacaagga
    35281 atcactgaat ctgtccgcac gaaaagcaat atggatggcg ttgaccagct gctgaaagtt
    35341 atgaaaaacg atttttcagg aatcagcttt accaatttag tagattttga tgcattatac
    35401 ggtcatcgtc gggatgtagt cggctatgcg catgcgattg aagaatttga cttacgaatt
    35461 ccagaattgc ttgaagcgat ggaagaagac gacttgctaa tgattacagc cgatcacgga
    35521 aacgatccga cgtttactgg taccgatcat acgcgagaat atgtaccgtt attggtttac
    35581 agtaaaaaaa tgaaagaaca aggggcattg cctcaaggat attattcaga tatttcagca
    35641 acgattgctg agaactttag tgtttcttcg actgaaaatg gcgaaagctt cttgaaatta
    35701 ttaaaataat ctttttaaat aggagaaagc aaatgaccaa gttaagcgag atgttacaag
    35761 aaacgaccca gtttatcctt gataaaggag taaaagaaat tgattttggt ttgattctag
    35821 gttcaggatt aggtgaatta gcagatgaaa tcgaagatgc tgtaatgatt tcttatgatg
    35881 agatcccgtt ctttcctgtt tcgacagtag ttgggcatgc aggaaagcta gtttatgggt
    35941 ctctggcagg caagaaagtg ttagccatgc aagggagatt ccactactat gaaggtcatt
    36001 ccatgcaaac agtgacctat ccagtacgtg tgatggccgg actaggggcc cattcagtca
    36061 ttgtgactaa tgcatgtggt ggtgtcaacg aatcatttgt accaggagat ttgatgctga
    36121 ttaccgatca tatcaatttt actggtgaaa atcctttgat cggtccgaat gaagatgcaa
    36181 tgggccctag atttccagat atgagtcaag cttatacgcc tgactatcaa gcaacagcaa
    36241 aagcagcagc agaaaaacta ggtttggatc ttaaagaagg cgtatatatg ggctattctg
    36301 gaccaactta tgaaacgcct gcggagatcc gaatgtctcg gacaatagga gccgatgcag
    36361 tagggatgtc gactgttcca gaagtcatcg tagcagctca cagcggatta aaagtgttag
    36421 gaatctcttg tattaccaac ttagcagcag gtatgcaagc gaatctgagc catgaagaag
    36481 ttgtagaaac aactcagaga gtcaaacaat cttttaaagc attggttaaa gaaacattga
    36541 cattattgta ataggaggaa caataaaatg agtgtgcata ttgaagcgaa agaaggcgag
    36601 atcgccgata agattttact tccgggagat cctttgcgag ctaaatatat tgcggagact
    36661 tttttggaag atccagtttg ttacaatcag gtacgcggta tgttaggctt taccggcaaa
    36721 tataaaggag aacgtatttc tgttcaagga acaggaatgg gaatgccatc agccaccatc
    36781 tatgcccatg aattgatcca gtcgtatggt gtaaagaaat tgattcgagt aggtacatgc
    36841 ggtgcattgt caaaagacgt ccatgttcgt gatttagtac tagcacaagg tgcagcgacc
    36901 agttcttcga tgatcgaaaa aaatttccaa gcctttcatt ttcctccaat cagtgatttc
    36961 aatctattac tgaaagcata tgagatagct aaagaaaagg gctatacggt tcatgtagga
    37021 aatgttcttt cggaagattc tttctacaaa gatgatttga cagagacgtt ccaattagct
    37081 gaacttggcg ttttaggagt ggaaatggaa gcagcggctc tttattactt aggcgcgaaa
    37141 taccatgtgc agacattaag tttgatgacc gttagcgacc atttgatcac cggtgaagaa
    37201 acaacagctg cagaacgtca atctacgttc aatgaaatga tcgaagtagg attggaaaca
    37261 gcgatagcag aataagaagg aaatgaaaaa gctgccctgc attaagtaat aagaacatcc
    37321 aagcaaaaat agctcctcat gtttttgact tggctggact tattattgca aatgtaagct
    37381 tttgaacacc gtttaccaag aggttgtgaa tctgccgttt aactctgagt cacaaggaac
    37441 aatttcaaaa aacagctttt catgtttgat gaatattgtg acttgtgacc gagaagttgg
    37501 caggagaaaa ccgtttatac gaaaaaaggg actgtgacaa atcgtgtcac agtccctttt
    37561 ttatagtgtt ttttataggt agtatttttt agcaactgtt aagtcatcat ttaattcata
    37621 aacaagtggt tgacctgttg gaatttcaag atccatgatg tcttcgtcag agataccttc
    37681 gatatgttta gctagggcgc gtaatgagtt accatgtgca gctactaaaa cagttttgtt
    37741 gtctagtaat gctggagcaa tttcatcttg ccagaatggc aaagcgcgtt ctaatgtcac
    37801 tttcaagttt tctccaccag gaatatcgcg tttgtctaac attgcgtaac gacggtcatt
    37861 tgctgcagaa ccttcgtcag tttcttccat taatggagga agagtatcgt aagaacgacg
    37921 ccagatgtgc acttgttcgt cgccgtattt ttcagctgtt tcttttttgt tcaaaccttg
    37981 taatttacca tagtgacgtt cgtttaagcg ccatgatttg atttgtggta cccaaagttg
    38041 atctgaatat tctaagatca agttacatgt tttgattgca cgagttaaaa cagaagtgta
    38101 agcaacatca aattcgattc cagcttcttt gattttgcgt ccgccttctt ttgcttcttc
    38161 gattccttct ggtgctagat ttacatcagc ccaaccagta aattggttca atgcattcca
    38221 ttcactaaga ccgtgacgag aaaaaactaa ttttggcatg tttagctctc cttttaagat
    38281 aggtttatat ttctgaaatt ttcagttctg ttttattgta caattttttg gaagagattt
    38341 ccatcgaaaa attagataag attctatgag aaaacaagta gagaatataa aaaaacgaac
    38401 agtgcccacg caactgttcg tttttaggta ttagatgcat aattgctcga tttctttttc
    38461 aggtaaattg atatatttat aacatgttcc aactgaaaca ccgcattttg aagaaataaa
    38521 ttggatcgtt tctttttttt cataatagag acgtcggatt tttttgatcg tttttgcatc
    38581 gatttttggt cttccgccga tttttccttt tttacgagct tcatctaatc caactaatgt
    38641 acgttctttg atcaatgcgc actccatgtt tgctaggttt tccattaatt ggaaatagat
    38701 ttttcccatt tcgcgcgtat cgattccttc atcaagacta atcaagtgca gcccgcgatc
    38761 tttgaataat tgcgtgagtt ctgttaattg ccgcgtagat ttgcctaatc gatgaagttg
    38821 gcaaatgact agtgcatcgc cttgttgcat gtcagcgatc actgactcca aatgaacagc
    38881 agtagaatca ttttgttcca cttcatatgt ctcatggaaa atttcttgac aaccaaagtc
    38941 agataattga tcaacctgag ttgttaaatc aacgtcattt atggttgtgc gtgcataacc
    39001 aataattttc atggtgattc ctttctagca gtgattttct aactggatat aacagtaaaa
    39061 atgtagcata aagtacgtca aaaagatagt tttttcgcaa attttccgct gtcttttttt
    39121 attggatgat caagcagttt ttctttattt acgacggatt gcgtcaagaa tctcaacgcc
    39181 tttttctgag ccgacaacta ctttagaaac catgtttagg aaaagcccat gttctactac
    39241 cccaacaagt tcatctaaat aggtagctaa gctttctggc tgttcgatga cttctagatg
    39301 cagatcaatg atgtaatgac caccatctgt ggttaagact tctccgtttt cgtctgtacg
    39361 caagacgggt tggaatcttt tttcttcaaa aatatgaagt agctgttggg agccataagg
    39421 gatcacttca actgggagtg ggaacttccc aagtttatcg actaatttag aagaatcaac
    39481 gatccaaatc gtttcttttg agtacgtagc gactattttc tcgaaaagca aagcagcgcc
    39541 gccgcctttg atcccttgaa aatcagcact gatttcatca gcaccgtcta ttgtcaaatc
    39601 gactacgggg acttcatcaa tactctttaa cggtattcct aatttttccg cttgttcttt
    39661 tgtcgcattt gaagtagtaa ctcctgtgat acgtaaacct tcttcacgca tacggcgacc
    39721 aatctcttct accatataat aggcggttga accggtcccg agacctacga tcatcccgtc
    39781 ctttacgaat ttcgcggctt caatgccagc taattgtttt aaattcatct ttgcgtccac
    39841 ctttttttag tttgagcatc gtgctttcct cactatttta gcgtgattta cggtaaaaag
    39901 ataccattct tttttattcc aataaagctt tttcaaaaaa aattgcatct cttgttgaca
    39961 tgttttttca ttcgtgatat tctaacaaag gtgctttatc tacaggtttc tgtgaagaaa
    40021 cgtgctgact gtttatcaaa gcatatttga taaggatgca gacattgcat ttatttttta
    40081 tcacaaaaaa agaaccacct ggatgtgtgg aactagacgc aaatcgtggg aaggaggaaa
    40141 caaggaatgc ctacaattaa ccaattagta cgtaaacctc gtaaatcaaa ggtggaaaaa
    40201 tctaattcac ctgctttgaa caaaggatat aacagcttta agaaaactca aactaacgta
    40261 aactcacctc aaaaacgtgg ggtagcaact cgtgtgggta caatgacacc taaaaaaccg
    40321 aactcagctt tacgtaaata tgcacgtgtt cgtttgtcaa acttaattga agttactgct
    40381 tatatcccag ggattggtca taacttacaa gaacacagtg ttgtattatt acgcggtggt
    40441 cgtgtaaaag acttaccagg ggtacgttac catatcgttc gcggtgcttt agatactgct
    40501 ggtgttaacg accgtaaaca aagccgttct aaatacggaa ctaaaaaacc taaagcttaa
    40561 ttttaattat taaacaaatg aaacttgctt ctactatata gaagtaatta ctatgaacaa
    40621 tatttcggaa ggaggagtta cggatgccac gtaaaggtcc tgttgcaaaa cgtgatgttt
    40681 taccagatcc tatttataac tcaaaattag taactcgctt gatcaaccgt gtaatggttg
    40741 acggaaaacg cgggattgca gctaacatta tctataatgc ctttgacttg atcaaagaat
    40801 ctacaggtaa cgatccatta gaagtgtttg aacaagcaat gaaaaacgtt caacctgttc
    40861 ttgaagttaa agcacgtcgt gttgggggtt caaactacca agtaccagtt gaagttcgcc
    40921 ctgaacgccg cgttactcta ggtcttcgtt gggtagtaaa ctacgctcgc ttgcgcggtg
    40981 aacacacaat ggaagaacgc ttagcaaaag aaatcatgga tgctgcaaac aacacaggtg
    41041 cttctgttaa aaaacgtgaa gacactcata aaatggctga tgccaaccgt gcgtttgctc
    41101 actatcgctg gtaagattct ctctgtttgg tcggctttta taagtcgacc tcagatagtg
    41161 tccttatttt tagcaactag atagtaaaga gaggagaaac aaggagaatg accagagaat
    41221 tttctttaca aaacactcgt aatatcggta tcatggccca catcgatgct ggtaaaacga
    41281 ctgctactga acgtatcttg tactatactg gtaaaatcca taaaattggt gaaactcatg
    41341 aaggagcttc acaaatggac tggatggaac aagaacaaga acgtggtatt acgatcactt
    41401 ctgctgctac tacagcacaa tggaagggtc accgaatcaa catcatcgac acaccaggac
    41461 acgtagactt cacagttgaa gttgaacgtt cattgcgtgt acttgatggt gctgtaactg
    41521 ttcttgatgc acaatcaggt gttgaacctc aaactgaaac agtttggcgt caagcaacaa
    41581 cttatggtgt acctcgtatc gtatttgcaa acaaaatgga caagatcggt gctgacttct
    41641 tatattcagt atcaacttta catgaccgtt tgcaagcaaa cgctcaccca atccaattac
    41701 caattggtgc ggaagacgac ttcacaggaa ttatcgacct tgtaacaatg aaggctgaaa
    41761 tgtatacaaa cgatcttgga acagagatcg aagaaacaga aattcctgaa gaataccgcg
    41821 aattagctga agaatggcgc gaaaaattgg ttgaagctgt agctgaaact gatgaagaac
    41881 ttactttgaa atacttagaa ggtgaagaaa tcactgaagc tgagttaaaa gaaggtatcc
    41941 gccgcgcaac agttaacgta gaattctacc cagtactatg tggttcagca tttaagaaca
    42001 aaggggttca attactattg gacgctgttc ttgattactt gccatcaccg ttagatatcc
    42061 cagctatcaa agggattgac cctaaaactg atgaagaagt agaacgtcct gcagacgaca
    42121 gcgctccttt ctcagcttta gcatttaaag ttatgactga cccattcgta ggtcgtttaa
    42181 ctttcttccg tgtgtactct ggtgttttac aatcaggttc atacgtacaa aacgcaacaa
    42241 aaggcaaacg tgaacgtgtt ggtcgtatcc tacagatgca tgccaactct cgttcagaaa
    42301 tctctgaagt ttacgctgga gatatcgctg ctgctgttgg tttgaaagac acaactactg
    42361 gggacacact atgtgatgaa aagagtctag ttattcttga atccatggaa ttcccagaac
    42421 cagttatcca agtcgctatt gaacctaaat caaaagctga ccaagataaa atgggtgtgg
    42481 ctttacaaaa actatctgaa gaagatccta ctttccgtgc tgaaacaaac gtggaaacag
    42541 gcgaaacaat catcgctggt atgggtgaac ttaacttaga tatcatcgtt gaccgtatgc
    42601 gtcgtgaatt caaagttgaa gctaacgtag gtgcgcctca agtatcttac cgtgaaactt
    42661 tccgtgctgg cactcaagcc gaaggtaagt ttgtacgtca gtctggtggt aaaggtcaat
    42721 acggtcacgt atggattgaa tttacaccaa atgaagaagg tgctggtttc gaatttgaaa
    42781 atgcgatcgt cggtggggtt gttcctcgtg aatacattcc agctgttgaa actggtttga
    42841 aagatgctat ggaaaatggt gtcttagctg gctatccatt agtagatatc aaagctaaac
    42901 tttacgatgg ttcataccat gatgtcgatt caaatgaaac tgccttccgt gttgctgctt
    42961 ctatggcatt acgtgcagca gctaagaaag ctaatccagt aatccttgaa ccaatcatgg
    43021 ctgtagaagt tgttatccct gaagattatt taggtgatgt tatgggtcac gtaactgctc
    43081 gtcgtggacg tgttgaaggt atggaagctc gcgctggcgg ccaacaagtt gttcgtgcga
    43141 tggttccact tgctgaaatg tttggttatg ctacaactct tcgttctgct actcaaggtc
    43201 gtggtacatt tacaatgaca tttgaccact acgaagatgt accaaaatca gtacaagaag
    43261 aaatcattaa gaaaaacggc ggaaaagctg aataatttct taaaaccgga tagtcctgca
    43321 aagagattat ccaaagagac tatattttta atatagtttc gtgtaaaata attatcgatg
    43381 atatgggcat gtggtgtacc ttgtgtacat catataccta tcagacaaat aaaaaatata
    43441 ttgtattttt aggaggacat tttaaaatgg caaaagaaaa atttgaccgt tctaaaccac
    43501 acgttaacat tggtactatc ggacacgttg accatggtaa aactacatta acagctgcaa
    43561 tcacaactgt actatctaag aaaaacggcg gccaagctat ggcttacgat caaatcgatg
    43621 gtgctccaga agaacgcgaa cgcggaatca caatctcaac agctcacgtt gagtatgaaa
    43681 cagatacacg ccactacgct cacgttgact gcccaggaca cgcggattac gttaaaaaca
    43741 tgatcactgg tgctgctcaa atggacggag ctatcttggt agtttctgct gctgacggcc
    43801 caatgcctca aactcgtgaa cacatcctat tgtctcgtca agttggtgtt ccttacatcg
    43861 ttgtattctt gaacaaagta gacatggttg atgacgaaga attactagaa ttagttgaaa
    43921 tggaagttcg tgacctatta acagaatacg aattccctgg tgacgatgtt cctgtagttg
    43981 ctggatcagc tttgaaagct ctagaaggcg acgcttcata cgaagaaaaa attcttgaat
    44041 taatggctgc agttgacgaa tacatcccaa ctccagaacg tgacaacgac aaaccattca
    44101 tgatgccagt tgaagacgtg ttctcaatta ctggacgtgg tactgttgct acaggtcgtg
    44161 ttgaacgtgg acaagttcgc gttggtgacg aagttgaagt tgttggtatt gctgaagaaa
    44221 cttcaaaaac aacagttact ggtgttgaaa tgttccgtaa attgttagac tacgctgaag
    44281 ctggagacaa cattggtgct ttactacgtg gtgttgcacg tgaagacatc caacgtggac
    44341 aagttttagc taaaccaggt acaatcacac ctcatacaaa attctctgca gaagtatacg
    44401 tgttgacaaa agaagaaggt ggacgtcata ctccattctt cactaactac cgtccacaat
    44461 tctacttccg tacaactgac gtaacaggtg ttgttgaatt accagaagga actgaaatgg
    44521 ttatgcctgg cgacaacgta acaatggaag ttgaattaat ccacccaatc gctatcgaaa
    44581 acggtactaa attctcaatc cgtgaaggcg gacgtacagt tggtgccggt gtcgttacag
    44641 aaatcaaagc ttaattcact taagttttaa agtaaagcaa catttattcg gacgtaatcg
    44701 ctcggaacga aggtcttagg atctttgttc cgagcttttt tatttattgc aggatcagga
    44761 aaaaagacag gagtattata aatagaagaa acacgaaata aaagagtcag tattttctaa
    44821 atgaaacttt atgaaaacaa aatgtttttt acaagaaaaa gccttgcttt gtgagtttat
    44881 atatattata atgacaaagg tgcgtttact ttagtgaatt gcacagacgt aatcagaatg
    44941 atttcgtcgg ctaagaaacg agaggttgcg acacgcccgg acgctttgcc acgaacgagc
    45001 gtgacggaaa ttttcgtgga gctatgtcta cttttcaaaa taggcgaagg agggaacaag
    45061 atggcaaaac aaaaaattcg tatccgttta aaagcgtatg aacaccgtat tttagatcaa
    45121 tcagcggata aaatcgtgga aactgcaaaa agaactggag ctagcgtgtc tggtccaatt
    45181 ccattaccaa ctgaacgcag tctttacaca atcatccgtg cgactcataa atacaaagat
    45241 tcacgcgaac aattcgaaat gcgtacacac aaacgtctta tcgacattgt gaacccaaca
    45301 ccaaaaactg ttgatgcttt gatgaagcta gacttaccat ctggtgtaaa catcgaaatc
    45361 aaattataaa actaaaacaa aaataatgga ggtgtactca tgaccaaagg aatcttaggg
    45421 aaaaaagtgg gaatgacaca aatcttcact gaatctggtg aattaattcc agtaactgtt
    45481 gtagaagcta ctccaaacgt agtactacaa gtgaaaacaa tggaaacgga tggctacgaa
    45541 gctattcaag ttggttacca agatatgcgt gaagttttat caaacaaacc tgcgaaaggt
    45601 catgttgcaa aagcaaacac ggctcctaag cgcttcattc gtgaattcaa aaatgttgag
    45661 ctaggagaat atgaagtagg aaaagaaatt acagtagatg ttttccaagc aggagacatt
    45721 attgatgtaa caggtactac gaaaggtaaa ggattccaag gggttatcaa acgccacgga
    45781 caaagccgcg gaccaatggc tcacggttct cgttaccatc gtcgtcctgg gtcaatgggt
    45841 ccagttgcac ctaaccgtgt atttaaaaac aaacgtcttg ctggccgtat gggtggcaac
    45901 cgcgtaacta tccaaaactt ggaagttgta cgtgtcgatg cagaaagaaa tgttatcttg
    45961 atcaaaggta atgttcctgg agctaaaaaa tctttaatta ctatcaaatc agctgtgaaa
    46021 gctaaataat tgtaggaaga aaggaggaac taaggaatgc cgaatgtagc attattcaaa
    46081 caagatggaa gccaaaacgg tgaaatcaca cttaacgaag aaatcttcgg aattgaacct
    46141 aatgaaagtg ttgtttacga tgcaatcgta atgcaacgtg cgtcattaag acaaggaaca
    46201 cacgcggtta aaaaccgtag cgctgttcgc ggcggcggac gtaaaccatg gcgtcaaaaa
    46261 ggaacaggtc gtgcacgtca aggatctatc cgctcaccac aatggcgtgg aggtggcgta
    46321 gtctttggac caacaccacg ttcttacagc tacaaacttc ctaaaaaagt tcgtcgttta
    46381 gcaatgaaat ctgtattgtc agaaaaagtt gctgaaaaca acttggtagc aatcgaaggc
    46441 ttgaacttcg acgcaccaaa aacaaaagaa ttcaaacaag ttcttgctaa cttatctatc
    46501 gactctaaag tattggttgt tctagaaaaa ggtaacgact ttgctgcttt atcagcacgt
    46561 aacttaccaa acgtttctgt agtaacttct gataatgtga gtgtgctaga cgttgtatca
    46621 aatacaaaag tgttagcaac acaaactgct cttactcaaa ttgaggaggt gcttgcataa
    46681 tgaacttact agacgtaatc aaacgcccag tgatcactga aaaatcaatg cttgccatgg
    46741 atgacaagaa atacactttt gaagtggaca ctcgcgcaaa caaaacttta gtaaaacaag
    46801 cggtagaagc tgcttttgac gttaaagtca aaaacgtgaa catcgtgaac gtacgtgcga
    46861 aattcaaacg tatgggcaaa tatgcaggat atacaaaaaa acgtcgcaaa gcgatcgtta
    46921 cattgactga agattcaaaa gaaatccaat tattcgaagc tgctgaataa gcactaatag
    46981 ataactcatt aaattaggag ggaaaaacgt ggcgattaaa aagtacaaac ctacctcaaa
    47041 tggtcgtcgt aacatgacag gttctgattt tgctgaaatc acaacatcaa cacctgaaaa
    47101 atcattgtta caaccattga aaaaccatgc tggacgtaac aacaacggcc gcatcactgt
    47161 acgtcatcaa ggtggcggac acaaacgcca atatcgcgtg atcgacttca aacgtaacaa
    47221 agataacgtt gttgcaactg ttcaaacgat cgaatatgat ccaaaccgtt cagctaacat
    47281 cgcgttagta cactatgaag atggagtgaa agcttacatc ttagcaccta aaggactaga
    47341 agtgggtatg aaacttgttt caggtccaga agcagatatc aaagtcggaa atgctcttcc
    47401 attagaaaac attccagttg gtactgttat ccacaacatt gaaatgaaac ctggtaaagg
    47461 tggacaattg atccgttctg ctggtacaag tgctcaagta cttggtaaag aaggcaaata
    47521 cgtattgatc cgcttgaact ctggtgaagt tcgtatgatc ttagctgcat gccgtgctac
    47581 aatcggttct gtaggtaacg aacaacacga attaatcaac atcggtaaag ctggacgctc
    47641 tcgttggatg cgtaaacgcc caactgtacg tggtagcgta atgaacccta atgatcaccc
    47701 acacggtggt ggtgaaggta aagctcctat cggacgtaaa gcaccagttt ctccttgggg
    47761 tcaaccagct cttggttaca aaactcgtaa caaaaaagct aaatcagaca aacttattgt
    47821 tcgtcgtcgc aaaaataaat aattactttg accatgtgtc gcacattttg agaggaggtt
    47881 caccatgggt cgtagtttaa agaaaggacc ttttgtcgat gagcatttaa tgaaaaaagt
    47941 tgaagctcaa gcaggggttg aaaagaaaaa agttatcaaa acttggtcac gccgttcaac
    48001 agttttccca agttttgttg gatataccat cgcagtttat gatggacgta aacatgtacc
    48061 agtttacatc caagaagaca tggtaggaca caaattaggt gaatttgcac ctacaagaac
    48121 ttatcgtgga catgctgctg acgataagaa aacgaaacgc taatttgagg ggaggataaa
    48181 tccaaatggc agaacaaatc acatcagcta aagcaactgc aaaaacagtt cgcacttcac
    48241 ctcgaaaagc ccgtttagta attgacctta tcagaggtaa aagtgtcgca gatgcaattt
    48301 caatcttgaa attcacacca aacaaatctg ctggaatcat tgaaaaagta ttgatgtcag
    48361 cagttgcgaa tgcagaaaat aactttgact tagatgttga aaacttggta gtaagcgaag
    48421 cttttgttaa cgaaggacct acaatgaaac gtttccgtcc tcgtgcaaaa ggatcagctt
    48481 ctccaatcaa caaacgtaca agtcatatca cggtagtcgt atcagaaaaa taaggaggga
    48541 taatcagtgg gtcaaaaagt acatccaatt ggaatgcgtg taggcatcat ccgcgactgg
    48601 gatgctaaat ggtatgctga aaaagagtat gctgagttct tacacgaaga tttaagaatc
    48661 cgtaaattta tttcaactaa acttgctgat gctgctgtat caacagttga aatcgaacgc
    48721 gcaacaggtc gcgtaaacat ttcaattcac acagctaaac caggtatggt tattggtaaa
    48781 ggcggttctg aagtcgaaag cctaagaaaa gaattaaaca aattaactgg taaaagaatc
    48841 catatcaata tcgtggaaat caaaaaacca gatctagatg caaaattagt aggtgaagga
    48901 atcgcacgtc aattagaaaa ccgtgttgct ttccgtcgcg ctcaaaaaca agccatccaa
    48961 cgctcaatgc gtgctggcgc tcaagggatc aaaactcaag tatctggtcg tctaaacgga
    49021 gcagatatcg ctcgttcaga aggatactca gaaggaactg ttcctcttca tactttacgt
    49081 gcggacattg attacgcatg ggaagaagca gatacaacat acggaaaact aggagttaaa
    49141 gtgtggatct atcgtggaga aattcttcca actaaaaaaa acactgagaa aggagggaaa
    49201 taatcatgtt agtacctaaa cgtgtaaaac accgtcgtga attccgcgga aaaatgcgcg
    49261 gggaagctaa aggcggaaaa gaagtagcat tcggtgaata cggtttgcaa gctgttgatt
    49321 cacattggat cacaaaccgc caaatcgaag ctgctcgtat cgcaatgact cgttacatga
    49381 aacgtggtgg gaaagtatgg attaaaattt tccctcacaa atcttatact gccaaagcaa
    49441 ttggggtacg tatgggttct ggtaaagggg cacctgaagg atgggttgca ccagtaaaac
    49501 gtggtaaaat catgtttgaa atcgcaggcg ttcctgaaga agtagctcgt gaagcgttac
    49561 gtctagcttc tcacaaatta ccaatgaaaa ctaagatcgt aaaacgtgag gaaatgggtg
    49621 gtgaatcgaa tgaaggttaa agaaatcaga gaattaacca ctgccgaaat gcttgatcaa
    49681 gaaaaacaat taaaagaaga attgtttaat cttagattcc aattagcaac aggtcaatta
    49741 gaaaacactg cacgtattaa agaagtacgt aaatcgattg cacgcatcaa aactgtgttg
    49801 cgtgaacaag ctaagtaatt atggaaggag gccattaagc gtatgactga aggaagaaat
    49861 caacgcaaag tttatcaagg tcgcgtggtt tcagacaaaa tggataaaac aattaccgtt
    49921 gctgtcgaaa caaagaaaaa ccaccctatc tatggcaaac gcatgaacta ttctaagaaa
    49981 tacaaagtgc atgatgaaaa caacacagca aaagttggcg acatcgtgag aattatggaa
    50041 actcgtccat tatcagctac aaaacgtttc cgtttgttgg aagtagtcga agaagcagtt
    50101 attatctaat aacacgagat tttcctatat agattgaatg caaaatctga aaggaggata
    50161 cacaacgtga tccaagcaga aagtcgttta aaagtcgctg ataactcagg cgcacgtgaa
    50221 attttgacga tcaaagtcct tggtggatct ggtcgtaaaa ctgctaacat cggtgatgtg
    50281 atcgttgcta cggttaaaca agcaacgcca ggtggggttg tcaaaaaagg tgacgtcgtt
    50341 aaagccgtta tcgttcgaac aaaatcagga gctcgtcgta cagacggttc atatattaaa
    50401 tttgatgaaa atgctgcagt gattattcgt gatgataaaa gccctcgtgg aacacgtatc
    50461 tttgggccag ttgcacgtga actacgtgaa aacaacttca tgaagatcgt ttcactagca
    50521 ccagaagttt tataatcttg agacacttat caaaggaggt gcgaaacagt tatgtttgtt
    50581 aaaaaaggcg ataaagttaa agttatcact ggaaaagaca aaaacaaaga aggcgttgta
    50641 ctagcagcgt tcccgaaaaa agataaagtt attgtcgaag gtgtaaatgt cgttaaaaaa
    50701 caccaaaaac cttctcaagc tgctcctcaa ggtggaattg ttgagatgga agcgccaatt
    50761 catgtttcta acgtgatggt ggttgactct actggtgtag ctggccgagt tggctacaaa
    50821 gaagtagacg gcaaaaaagt ccgtgtttct aaaaaaaccg gtgaagtttt agataaataa
    50881 aagtttaagg aaggagggtc ttactgaatg aaccgcctaa aagaaaaata tcttaaagaa
    50941 gtaactccat cattgatgga aaaatttaac tatagctctg ttatgcaaac acctaaagtt
    51001 gaaaaaatcg tcatcaacat gggtgttggt gatgcggtat caaacgctaa aaacttagat
    51061 aaagcagttg aagaattaac tttgatcact ggtcaaaaac caatgattac aaaagctaaa
    51121 aaatcaatcg ctggattccg tttacgtgaa ggaatgccaa ttggtgcaaa agttacattg
    51181 cgcggagaaa gaatgtacga attcttagac aaattggttt ctgtttcact accacgtgta
    51241 cgtgacttcc atggtgtaag taaaaaagcc tttgacggtc gtggaaacta tactttaggt
    51301 attaaagaac aattgatctt cccagaagta gattatgatt tagtagataa agtacgtggt
    51361 atggacatcg ttattgtaac aacagcaaac tctgatgaag aatctcgtga gttgttgaca
    51421 caattaggca tgccattcca aaaataatta aaggaggcga actacgtggc taaaaaatca
    51481 atgattgcta aaaacaaacg tcctgcaaaa ttttcaacac aagcttatac tcgttgcgaa
    51541 cgttgcggac gtccacattc agtttatcgt aagttccatc tttgccgtat ttgcttccgc
    51601 gaacttgcct ataaaggtca aattcccggc gtgaagaaag ctagctggta aaaagtaaat
    51661 tcgcataaga aggaggtaaa agatcaatgg tcatgacaga tccaattgca gattttctaa
    51721 ctcgtatccg taacgcaaac atggttaaac atgagtcttt agaagtacct gcatcaaaaa
    51781 ttaaacgtga catcgctgaa atcttgaaac gtgaaggttt cgttcgtgat gttgaataca
    51841 tcgaagatga caaacaaggc gtgatccgtg ttttcctaaa atttggtaaa aacggcgaac
    51901 gtgttatcac aaacttaaaa cgtatctcta aaccaggttt gcgtgcttat gtcaaagctg
    51961 acgaagtacc aaaagtattg aacggtttag gtatcgcaat catttcgact tctgaaggtg
    52021 ttgtcactga taaagaagct cgtgaaaaaa atatcggcgg cgaagttatc gcttacgtat
    52081 ggtaatcaag tagaatctac aaggaggtgt ctttaagtga gccgtatcgg aaataaagtc
    52141 gttgttatcc ctgaaggagt aacagttact caagacggaa acaacgttac agttaaagga
    52201 ccaaaaggtg aattgactcg tactttttca gcagatatca aaatgaatat cgaaggaaac
    52261 gaagtaactt tcactcgtcc aaacgacagt aaagaaatga aaacaattca tggaacaact
    52321 cgtgcgaact tcaacaacat ggttgtaggt gtcagcgaag gtttccaaaa agcgttagaa
    52381 ctaatcgggg ttgggtaccg tgcacaatta caaggatcaa aacttgtatt gaacgttggt
    52441 tactcacatc cagtagaaat cacaccacca gctggagtaa ctgttgaagt accagctaac
    52501 acacaagtaa ttgttaaagg tgcgaacaaa gaagaagttg gcgaattagc tgcaaacatt
    52561 cgtggaactc gtcctccaga accttataaa ggtaaaggta ttcgttatgt tggcgaatat
    52621 gtacgccgta aagaaggtaa aactggtaaa taatcgttta gaaaaattgc atagaagcca
    52681 gcagcaggaa aaaggcattt ggctgaagtt taccttcaaa aaaatacatt tttacttgtg
    52741 ccgcagcttc tatttttcga aacgtattta ctgcttgaaa tagtagtaca ataaaaataa
    52801 agaggtgaca attgtgatta caaaaccaga taagaacaaa acacgtcaaa agagacatcg
    52861 tcgtgtacgt aacaatatct ctggtactgc tgagcgccca cgcttgaacg tttttcgttc
    52921 taacaaaaac atctacgctc aaattattga tgacgtagcg ggtgtaacgc tagcaagtgc
    52981 ctctaccttg gataaagaaa tttcaggtgg aacaaaaaca gaagcagcac aagctgtcgg
    53041 taaactagtt gctgaacgtg cagctgaaaa aggcattaaa gtagtagtct ttgaccgtgg
    53101 tggatacctt taccatggcc gtgtgcaagc tttagctgaa gctgctcgcg aaaatggact
    53161 agaattttag aagaaggagg aataccattc atggtttata tcgatccaaa acatttggaa
    53221 ttagaagacc gcgttgttgc aattaaccgt gtaacaaaag ttgttaaagg tggacgtcgt
    53281 ctacgttttg ctgctttagt tgttgtcggt gacaaaaacg gacacgtagg gtttggtaca
    53341 ggtaaagctc aagaagttcc tgaagcaatc cgtaaagcaa ttgaagatgc gaagaaaaac
    53401 ttagttgaag tacctatggt tggttcaaca atcccacacg aagtaatcgg tgcttttagt
    53461 ggtggtagaa tcctaatgaa acctgctgta gaaggttctg gggttgctgc tggtggacca
    53521 gttcgtgccg tattagaatt agctggggta gcagatatca cttctaaatc tttaggctca
    53581 aatacaccaa tcaacgttgt tcgcgcaaca gttgaaggtt tgaaacaatt gaaacgtgca
    53641 gaagaagttg cagaacttcg cggcaaatca gttgaagaat taatcggata aggaggacaa
    53701 caataatggc tgaattaaaa gttactttaa aacgcagtat tatcggacgt cctcaaaacc
    53761 aacgcgatac agttaaagcg ttaggtctta caaagattaa cagttcagtg gttaagcctt
    53821 ctaatgaagc aattaaaggc atgatcaata ctgtttctca cctagtggac gttgaagaaa
    53881 tttaatttag aaaaatcata actagaacat taaggaggtg ccaaacctat gaaacttcat
    53941 gaattaaaac ctgctgaagg ttcacgccaa gtacgtaacc gtgttggtcg tggtacttca
    54001 tctggtaacg gaaaaactgc cggacgcgga caaaaaggac aaaaagctcg ttcaggcggc
    54061 ggtgtgcgtt taggatttga agggggacaa acaccattgt tccgtcgttt accaaaacga
    54121 ggattcacaa acgtgaaccg taaagactat gcagtcatta atcttgatgt attgaaccgc
    54181 tttgaagacg gagctgaagt aactcctgtt gctttagttg aagctggaat cgtgaaaaac
    54241 gaaaaagctg gaatcaaagt attagctaat ggggaactaa acaagaaatt aactgtgaaa
    54301 gcagcaaaat tctctaaagc agctcaagaa gcaattgaag cggctggtgg atcaatcgag
    54361 gtgatctaat gtttagacta ttaaaagatg cttttaaagt aaaagacatt agatcgaaaa
    54421 tcctatttac tgtatttgtg ttgtttgtat ttcgcctagg tgcacatatt actgtaccag
    54481 gcgtaaatgc tgcttcattg caaaaccttt cttctttgcc atttttcaac atgttgaact
    54541 tggtaagtgg tagtgcgatg caaaactttt ccattttctc aatgggggtt tcaccataca
    54601 ttactgcttc tatcgtcatt cagcttcttc aaatggacat cgtgcctcga tttgttgaat
    54661 ggtcgaaaca aggggaagtt ggtcgtaaaa aactgaatca agcgactcga tatctgacat
    54721 tagtattggc atttatccaa tctgtcggta tcactgctgg attcaatcaa tacgctcaat
    54781 taggtttcgt gaataatcct aacgtagcaa cttacgtaac gatcggtttg attctaacag
    54841 ccggtacgat gtttgttact tggctaggag aacagatcac agaaaaaggt atcggaaatg
    54901 gcgtatcaat gatcattttt gcaggtatca tttctagatt gcctgaaagt atcagagaaa
    54961 tttatgaaga ttactttata aatattgatc cttcagacat atggaaatct gctatttttg
    55021 tggccatttt actagtagcg gtcttgttga tcgtgacatt cgtaacattt ttccaacaag
    55081 ctgaacgcaa gatcccgatc caatatacga aacgcgtagc tggcgcgcct acaagcagct
    55141 atctgccgtt gaaagtaaat gcagctggag ttattccagt tatctttgct agttcgttta
    55201 ttgctacgcc aaatgctgtt ttacaagcat tgagcggtag attcggttct gaaaactggt
    55261 acaaagtgat gatggagatc tttaactata atacagcacc tggtgctacg atttatacga
    55321 ttctgatcgt tgccttcact ttcttctacg cctttgttca agtaaaccct gagaaattag
    55381 cggaaaactt gcaaaagcaa ggaagttata taccaagcgt acgccctggt aaggggactg
    55441 aagagtatgt ctcaagatta ctgatgcgtt taagtacagt tggtgcagtc ttccttggtt
    55501 tagtggcatt gcttccaatc attgctcaaa tggtttggga tcttccacaa tcaatcggat
    55561 taggcggtac aagcttactg atcgttatcg gtgtagcttt agaaactgcg aaacagttag
    55621 aaggcttaat gctgaaacgt aaatatgttg gattcattga gtaagaaagc gtgttgaggt
    55681 ttcccctcaa cacacgttct cacacattta ttaggaggga aaacaatgaa cctcatttta
    55741 atggggctgc caggcgcagg taaaggaaca caagctgaaa aaatcattga tgcttatggt
    55801 attccgcaca tctcaacagg agatatgttc cgcgcagcga tgcaaaacga aacagctctt
    55861 ggcctggaag caaagtctta tatggataaa ggtgcattag ttcctgatga agtaacaaat
    55921 ggaatcgtga aagagcgact agctgaacca gatacagaga aaggcttctt attagatggc
    55981 tttccacgta cgctagatca agcaaaagct ctggacgcaa tgctaaaaga tttgaataaa
    56041 aaaattgatg ctgtcatcga tatccatgtt ggtgaagaaa tcttggtcga acgtctagcc
    56101 ggccgattta tctgcagcaa ttgtggagca acttaccata aaattttcaa ccctacaaaa
    56161 gttgaagaca catgtgatcg ttgcggtggg catgaattct atcaaagaga agatgataaa
    56221 cctgagacgg ttaaaaatcg tctggctatc aacatcaaaa atagtgaacc aatcttggct
    56281 tattataaag aacaaggttt gctaaacact attgatggtg ctcgtgaaat cgatgctgta
    56341 tttgcagatg tacaaaaaat cattgaaaaa tagcacgatt gcccgtagcc gacttgcatt
    56401 atgcagttac tcttgtcaag ttcaataaat tatgatagaa tatgtcagtc cgacttctag
    56461 agaatcctct agatgatcca ttttgagaat tttgaggtgg gaactgcagt ttccgccatt
    56521 ttatcgtgtg aaaatgcgaa acaagtacaa ggaggtactg tgcgtggcaa aagaagatat
    56581 gattgaagtc gaaggtacag tcgtcgaaac tttgccgaat gcaatgttta aagttgaact
    56641 agaaaacgga caccaagttc ttgctactgt ttcaggtaaa attcgtatgc attacatccg
    56701 tatcttacct ggtgacaaag taacggttga attgtctcca tacgatctta accgtggccg
    56761 tattacttat cgctttaaat aattgtactc cgtaaaacta caggaggtat tatcatgaaa
    56821 gtaagaccat cagtaaaacc aatgtgcgaa cattgtaaag taattcgtcg taaaggacgc
    56881 gttatggtga tttgcccagc aaatccaaaa cataaacaac gtcaaggata attggaggtg
    56941 ctataaatat ggctcgtatt gcaggagtag atattcctcg tgataaacgt gtagtagttt
    57001 cacttactta tatctatggt attggtaaca ctactgctaa aaagatctta gctgatgctg
    57061 gtgtatctga agatgttcgc gttcgtgaat taacgaatga acaaacagat gctatccgtg
    57121 cagaagtaga taaattaaaa gttgaagggg atcttcgtcg tgaagtgaac ttgaacatca
    57181 aacgtttgat ggaaatcggt tcttaccgtg gaatccgtca tcgccgtgga ttgcctgttc
    57241 gtggacaaaa cacgaaaaac aatgcacgta ctcgtaaagg cccagctcgc gctatcgctg
    57301 gcaagaaaaa ataattctta agtgaaggag gttaaaactt catggcagca aaaaaagtga
    57361 atcgtaaacg ccgtgtgaag aagaatatag aagcaggtat tgcacatatc cattctacat
    57421 tcaataacac tatcatcatg atcactgata ctcacggaaa tgcattagcg tggtcatcag
    57481 ctggttcatt aggtttcaaa ggaagcaaaa aatcaactcc ttttgccgct caaatggcag
    57541 cagaaactgc tacaaaagca gcaatggaac acggattaaa gactgttgaa gtaacagtta
    57601 aaggacctgg ttctggacgt gaagcagcaa ttcgttcatt acaagctaca ggtttagaag
    57661 tgactgcaat tcgtgacgtg actccagttc ctcataatgg tgcccgccct ccaaaacgcc
    57721 gtcgtgttta atgagtgccg ctcattttga gttttatcag taacgtcatt gaacaagaca
    57781 cttttcgttt tgaaaggggt aaagataaga atgattgagt tcgaaaaacc aagaatcgca
    57841 aaaattgatg aagaaaaaga ttatggcaag ttcatcgttg aacctcttga aagagggtat
    57901 gggactactt tagggaattc cctacgtcgt attttattat cttctttgcc aggtgctgcc
    57961 atcactaata ttcaaatcga tggtgtactg catgaattct ctaccatcaa aggtgttaga
    58021 gaagacgtca ctcaaatcat cttgaacatt aaaggcttag cgctgaaaat gtatggccaa
    58081 gaagaaaaaa cccttgaaat cgatattacc ggacctgcta cagtaactgc tggcgatatt
    58141 atcgttgata gtgatgtaga aattcttaac aaagatatgt acatctgtag tgtctctgaa
    58201 ggagctacgt tccatgctcg cttaacagtg aaacctggac gtggttatgt tcaagcagat
    58261 gaaaataaaa aagaagatat gccaattggc gttcttccag tcgattcaat ctacacacct
    58321 gttcgtcgtg tgaactatca agttgagaac acacgtgttg gacaccgtga agattttgat
    58381 aaattgacga tggagatttg gacagatggt tcaattgaac cgttggaagc aatgagtttg
    58441 tctgcgaaaa tcatgactga acatttggat atttttgtga acttaactga tgaagcaaaa
    58501 aatgctgaaa tcatggtcga aaaagaagaa acacaaaaag aaaaaatgtt ggaaatgacg
    58561 atcgaagagt tagacttgtc tgttcgctct tacaattgtt tgaaacgtgc aggaatcaat
    58621 actgtacaag aacttacaaa taaatctgag cctgaaatga tcaaagtccg taacttagga
    58681 cgtaaatcat tagaagaagt taaagcaaaa ttgcacgact taggtttagg tttacgtaaa
    58741 gaagattaaa accttttacg aaggagggaa accaacgtga gttatcgtaa actaggacgc
    58801 acatctagcc aacgtaaagc gatgttgcgt gatttaacaa ctgatttatt aatcaacgaa
    58861 cgtattgtga ctactgaagc tcgtgcgaaa gaagttcgct caactgcaga aaaaatgatc
    58921 actttaggta aacgcggaga tttgcatgca cgtcgtcaag ctgcggcttt cgtacgtaat
    58981 gaagttgcca gcgtgcgtga agaaaatgag gacatcgtca ttgaatcagc tttacaaaaa
    59041 ctgttcaacg atattgcacc tcgttacgca gaacgtcaag gcggatacac acgcattttg
    59101 aagacagaac caagacgcgg tgatggtgca ccaatggttg tcttagaact tgtctaaaat
    59161 cacactaata acatcacttt atagatgatt cttttagagt gttatgatgt tggaagatat
    59221 ttttccgagt ctagctcagc gctgctcctc aagcgaatat cgcttgggga gtaagcactc
    59281 atcataaagt gtttttttat accttcaatt acctgtcatg acagatatgt ttgaaggcat
    59341 aaaaaaacat tttaagcgag aaataattga aaattttgtc tttttttgat agtgttagta
    59401 ggaactttag gatgaagaag gagtaatcat ggagccaatt attgaattag aaaagataaa
    59461 ttataaatac cagccagatg atcttcgccc cgcattgaaa gatgtctcct ttacaattga
    59521 taaaggtgag tggatcgcta ttattggaca caatggttca ggaaaatcga cacttgccaa
    59581 aacgatcaat ggattgcttc tgccagaatc agggatcgtg aaagtgggaa atcaaatatt
    59641 ggatgaagaa aatatttgga cgatccgaca gatggttgga atggtttttc aaaatccaga
    59701 caatcagttt gttggttcga cagtagaaga tgacgttgct tttggtcttg aaaatcaagg
    59761 gattcccaga gaagaaatgc tagtgcgtgt gaaagatgca cttgagaaag ttagaatggc
    59821 tgagtttgct tcacgcgagc ctgctcgttt atcaggaggg caaaaacaac gtgttgccat
    59881 tgccggcgtg gttgccttac gacctgatat cattatttta gatgaagcaa ccagtatgct
    59941 agatccagaa ggtcgtgaag aagtgatctc tacgatcaag aagatcaaag aagaaagtca
    60001 attgaccgtt atatctatca ctcatgatat tgatgaagca gctaatgcca atcggatact
    60061 agtgatgaga caaggcgaac ttgtccgtga aggtacacca aaagaaattt tttctgctgg
    60121 tcctgaactg atcgacttag gtctagatct accatttcct gaaaaactga agagcgcact
    60181 gaaagaacgt ggggttgatg tgcccagtga atatatgaca gaagaaagga tggtggattg
    60241 gttatggaca tccgttttga acaagtagac ttcacttatc agccgaatac accatttgaa
    60301 caaagagcct tatttgacat caatatgacg atcaaagaaa acagttatac tgcattagta
    60361 gggcataccg gaagcgggaa atcaacctta cttcagcatt tgaatgcgct tgttaagcca
    60421 acaagcggta cagtccatat tggagaacga gatattcagc cggatacgga taataagaat
    60481 ttaaagccca tccgaaaaaa agttggcatc gtctttcagt ttccagaagc acagctattc
    60541 gaagaaacgg tagcaaaaga cattgctttc ggtcctaaaa actttggtgt cagcgaagaa
    60601 gaagcactag tcctagcaaa agaaacatta gaacaagttg ggctggatga aagctatttg
    60661 gaacgttcgc catttgaact ttcaggaggg caaatgcgta gagtagcaat cgctggtgtg
    60721 cttgccatga ggccggaagt acttgtattg gatgaaccaa cggcaggact tgatccccag
    60781 ggtagaaaag aaatgatgga gatgttttgg cgtcttcata aggaacagca gatcactatt
    60841 gtattagtta cgcatttaat ggatgatgta gccaattttg cagattatgt ctatgtgttg
    60901 gaaaagggac gaatcgttaa tagcggggag ccgcaagagg tctttcaaaa cattgaatgg
    60961 ctaaaagaaa aacaactagg cgtacctact gccaccgagt ttgcagaaga attaatggct
    61021 aaagggatga actttgctac cttgcctttg acagctgaag aattagcaga cgctatcgtc
    61081 cattatgctg gaggaaatgc ccatgatgaa taaattgata tttggtcgtt atatgcctgg
    61141 agattccttg atccatcgat tggatccccg agctaaatta ttagccagtt tttattttat
    61201 cggaatcatt tttttagcaa ataattggga aacgtacctt ttgttggcat ttttcacatt
    61261 actttctgtc ttcttgtcaa aagtagattt gaaatttttt atccgaggca ttcgcccatt
    61321 gatctggcta atcctcttca ctgtagcttt gcaggtcttg tttacaagtg gcggagaaac
    61381 ttattggagt tggggtgttt tccatatcac tgaatttggt gtgatgaacg gcctctttat
    61441 tttctgtcga ttcgtgttga tcatttttat gtcgactcta ctcacgctta ctacaccgcc
    61501 tttggaatta tcggatgcga ttgagtactt gcttcgtcca ttgaaagccg tacggttccc
    61561 agtccacgaa atttcattga tgctgtcgat tgctcttcgt tttgtgccta cattgatgga
    61621 cgaaacagaa aagatcatga acgctcagcg cgctcgcggt gttgattttg gtgaaggaaa
    61681 cctagttcag aaaatgaaag cagtcattcc tttgctgatt cctttatttg tcagcagctt
    61741 caaccgtgcg gaagatcttg cgactgctat ggaagcaaga ggctacaaag gtggggaagg
    61801 aagaacgaaa tatcgtgtat tgcattggca taatagagac acgatggtta tcattgcttt
    61861 tgtattatta actgtgctac tgatcttttt gagaggataa acgttcagtg ggccgctatc
    61921 tattcttttc ttgctgatct gtttgcaaga aaggatgcga gcagtgtatg acaagaaata
    61981 ttagggtctg tggcagacta aggtcacagg cctctttctt ttatctgaaa agaaagaacg
    62041 aaaaaatttt ataaaaaaat cattattcca gataaccttt taggaacaaa ggagagaaaa
    62101 acatggtacg ttacaaagca atcattgcat acgacggaac aaattttaat ggattccaaa
    62161 aacagccaaa tgggcgtact gttcaagaag aagtagaaaa aacattacaa aaaatggcaa
    62221 atggaaaaga aatcacggtc tttggttcgg gaaggacaga tgccggagta catgcgattg
    62281 ggcaagtaat ccattttgat tatccagagg agcgtccgtt agaacgaatg cgtttcgcgc
    62341 tagataccca gtcaccagag gatattgctg taagacaagt agaaattgtt tcagaagact
    62401 tccacgcaag atatttggtc aaagaaaaga cgtatcaatt tcgagtggat atagggaaac
    62461 cacgcagtcc ttttcgacgc cattatgcaa gttattttcc ttaccctctt gatttggaaa
    62521 aaattcagcg ggcgcttcct gatttattag ggactcacga tttcacctct ttttgtgcat
    62581 caggaagctc gatagaggat aaagtacgta cgatctacga ggctaagatg gaggtaaatg
    62641 aaacaaaaga cgaattgttg tttacattta ggggaaatgg cttcttgtat aagatgatac
    62701 ggatcttagt aggaacgctt ttgaaaattg gcaatggccg attgcctgaa gatagtatcc
    62761 cagatattat tgcgaaaaaa gatcgtaatg cagcaggacc tacagcacat cctgaaggac
    62821 tttatttgta tgaagtgaaa tatgaataag aggttgtgac agaagtggtc agcttcaaga
    62881 aataagaagg aatttacgaa aattacttct caaaattttg caaaatttcg gcttattttc
    62941 gaaggagcta cttctggaac accgtgaatc agaggttgtg aaaaagctgc cctgcatcaa
    63001 gtaataagaa caaccaaaca aaaatagctc gtcatatttt tcgtttggtt gggcttatta
    63061 ccgcagatgc aagcttttga acaccgttta aggaagaggt tgtgaaatca gcgttttgac
    63121 ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt tgttgagcta
    63181 ataccgaagg tcagctgatt gaacaccgtt taagaaagag gtcgtgagcc tgtcatttaa
    63241 ctctgagtca caagtaacaa tttcaaaaaa cagcttctca tctttgagga atatcgtgac
    63301 ttgtggacga ggcgttgata agtgaatcat ctttttgtta cctatttttt ctgtaaccaa
    63361 aaaacgatgg aacgttgttt tgattgccct taatgaggac agtcggaaca ggtttgtcat
    63421 ttttat
//
LOCUS       DACEUF010000005        49058 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.006, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000005 DACEUF010000000
VERSION     DACEUF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.006"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            4..816
                     /locus_tag="IXG20_000362"
     CDS             4..816
                     /locus_tag="IXG20_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ1608581.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IXG20_000363"
     CDS             813..1613
                     /locus_tag="IXG20_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ1608582.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IXG20_000364"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IXG20_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ1608583.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IXG20_000365"
     CDS             3110..4819
                     /locus_tag="IXG20_000365"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ1608584.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="IXG20_000366"
     CDS             5031..9383
                     /locus_tag="IXG20_000366"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ1608585.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="IXG20_000367"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="IXG20_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ1608586.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="IXG20_000368"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="IXG20_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ1608587.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="IXG20_000369"
     CDS             11221..11514
                     /locus_tag="IXG20_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ1608588.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="IXG20_000370"
     CDS             11511..11822
                     /locus_tag="IXG20_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ1608589.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="IXG20_000371"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="IXG20_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ1608590.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="IXG20_000372"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="IXG20_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ1608591.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="IXG20_000373"
     CDS             complement(14623..14844)
                     /locus_tag="IXG20_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1608592.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17654
                     /locus_tag="IXG20_000374"
                     /pseudo
     CDS             15292..17654
                     /locus_tag="IXG20_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18022..18945
                     /gene="truB"
                     /locus_tag="IXG20_000375"
     CDS             18022..18945
                     /gene="truB"
                     /locus_tag="IXG20_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ1608593.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18949..19890
                     /gene="ribF"
                     /locus_tag="IXG20_000376"
     CDS             18949..19890
                     /gene="ribF"
                     /locus_tag="IXG20_000376"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ1608594.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20242..21027
                     /locus_tag="IXG20_000377"
     CDS             20242..21027
                     /locus_tag="IXG20_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1608595.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21040..21834
                     /locus_tag="IXG20_000378"
     CDS             21040..21834
                     /locus_tag="IXG20_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ1608596.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKN"
     gene            complement(22014..22466)
                     /locus_tag="IXG20_000379"
     CDS             complement(22014..22466)
                     /locus_tag="IXG20_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1608597.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22931..23299)
                     /locus_tag="IXG20_000380"
     CDS             complement(22931..23299)
                     /locus_tag="IXG20_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ1608598.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23449..23931
                     /locus_tag="IXG20_000381"
     CDS             23449..23931
                     /locus_tag="IXG20_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ1608599.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            23968..24978
                     /locus_tag="IXG20_000382"
     CDS             23968..24978
                     /locus_tag="IXG20_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ1608600.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25093..25806)
                     /locus_tag="IXG20_000383"
     CDS             complement(25093..25806)
                     /locus_tag="IXG20_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608601.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(25996..26586)
                     /locus_tag="IXG20_000384"
     CDS             complement(25996..26586)
                     /locus_tag="IXG20_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ1608602.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27070..27297
                     /locus_tag="IXG20_000385"
     CDS             27070..27297
                     /locus_tag="IXG20_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ1608603.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27313..28479
                     /locus_tag="IXG20_000386"
     CDS             27313..28479
                     /locus_tag="IXG20_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ1608604.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28713..29756
                     /gene="hrcA"
                     /locus_tag="IXG20_000387"
     CDS             28713..29756
                     /gene="hrcA"
                     /locus_tag="IXG20_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ1608605.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29850..30413
                     /gene="grpE"
                     /locus_tag="IXG20_000388"
     CDS             29850..30413
                     /gene="grpE"
                     /locus_tag="IXG20_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ1608606.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30464..32293
                     /gene="dnaK"
                     /locus_tag="IXG20_000389"
     CDS             30464..32293
                     /gene="dnaK"
                     /locus_tag="IXG20_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ1608607.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32444..33610
                     /gene="dnaJ"
                     /locus_tag="IXG20_000390"
     CDS             32444..33610
                     /gene="dnaJ"
                     /locus_tag="IXG20_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ1608608.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(33692..34357)
                     /locus_tag="IXG20_000391"
     CDS             complement(33692..34357)
                     /locus_tag="IXG20_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ1608609.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            34333..34506
                     /locus_tag="IXG20_000392"
     CDS             34333..34506
                     /locus_tag="IXG20_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1608610.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(34551..35288)
                     /locus_tag="IXG20_000393"
     CDS             complement(34551..35288)
                     /locus_tag="IXG20_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1608611.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(35290..36792)
                     /locus_tag="IXG20_000394"
     CDS             complement(35290..36792)
                     /locus_tag="IXG20_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1608612.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            37051..39045
                     /gene="uvrB"
                     /locus_tag="IXG20_000395"
     CDS             37051..39045
                     /gene="uvrB"
                     /locus_tag="IXG20_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ1608613.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            39055..41871
                     /gene="uvrA"
                     /locus_tag="IXG20_000396"
     CDS             39055..41871
                     /gene="uvrA"
                     /locus_tag="IXG20_000396"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ1608614.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            42134..43399
                     /locus_tag="IXG20_000397"
     CDS             42134..43399
                     /locus_tag="IXG20_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ1608615.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            43403..44134
                     /locus_tag="IXG20_000398"
     CDS             43403..44134
                     /locus_tag="IXG20_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ1608616.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            44269..45153
                     /gene="rapZ"
                     /locus_tag="IXG20_000399"
     CDS             44269..45153
                     /gene="rapZ"
                     /locus_tag="IXG20_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ1608617.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            45150..46148
                     /locus_tag="IXG20_000400"
     CDS             45150..46148
                     /locus_tag="IXG20_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ1608618.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            46182..47117
                     /gene="whiA"
                     /locus_tag="IXG20_000401"
     CDS             46182..47117
                     /gene="whiA"
                     /locus_tag="IXG20_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ1608619.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            47660..48310
                     /locus_tag="IXG20_000402"
     CDS             47660..48310
                     /locus_tag="IXG20_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608620.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            48497..>49058
                     /locus_tag="IXG20_000403"
     CDS             48497..>49058
                     /locus_tag="IXG20_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ1608621.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        16408 a         8404 c        10563 g        13683 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatcctg tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa acccgaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatattga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaaacgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga acctttcttt attgaaggaa atgattcaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa ttaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaatcgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt tttgcaagtt
    16741 acgaatcatt cttgcgagtg gttgactcta tgttgactca gcacttcaaa tggatgcgga
    16801 aatcacatga cctttcttgg cgaaatgatt atccttcgct aaatctcatt gcaagctcaa
    16861 cagtcttcca gcaagatcac aatggatata gccaccaaga tccaggaata ttgacacatt
    16921 tggcagaaaa gaaggcggag ttcatccgtg aatatctgcc tgcggatgca aatacattgc
    16981 tcgcagtcat ggataaagca ttccgctcgt cagaaaaaat caatttgatc atctcttcca
    17041 aacatccgcg tgctcaattt tactcagcag aagaagcagc cgtcttagtg aatgaaggct
    17101 tgaaaatcat tgattgggct tcgacagcaa aagaggagga acctgaattg gtcattgctg
    17161 ctgcaggaac ggaatctaac ttagaagcat tagcagctgt aactttgctg ctggaagaat
    17221 tcccaaaact aaaaattcgg ttcatcaatg tcgtagattt attgaaatta cgtcatccaa
    17281 gtcaagatcc gcgtgggttg agcgatgaag aatttgatca atattttaca aaagacaaac
    17341 cgatcttatt tgctttccac ggttatgaaa cattggtccg cacgattttc tttgatcgtc
    17401 ataaccatca tttaatgatt catggctaca aagaaaatgg agatatcacg acaccatttg
    17461 acatgcgtgt agtaaatgaa cttgatcgtt atcatttggc aaaagacgca gctttaaaaa
    17521 tcaaaggaag tcaagcagaa gattttgcga aaaaaatgga tcagaaatta caagaacatc
    17581 aaaactatat tcgtgaaaat ggtattgatc tgccagaagt attggattgg aaatggaaaa
    17641 atcttgacca ataaaatgat aaaatcgatc ccgcaaaata gaatttttta cattgattaa
    17701 gaaactgaaa aaataaataa aaaaaacgga aaaaagactg gaagaatggc cagtcttttt
    17761 tttcgttttt gggaagtgaa aaccaaacta tttacctgta agccacccat aaatccaatc
    17821 aaatggtgca aagaaaaaat cttccattct attccctcct ttaaaaaagc ataacatgat
    17881 ttctatttac gtgagcatga attttgttac gtttttattg ccgaaaaata aaaaccgttc
    17941 attatgatgt caaattcgtc agtaattgat gcaatgtgtt ataataattc agttaaatca
    18001 aaaatgaaat ggagaaaaga catggacggg ttattacctt tatggaaaga acgcggaatg
    18061 acaagtcatg attgcgtatt caaattaaga aaaatcttac atacgaaaaa aatcggacat
    18121 gggggtacgc ttgacccaga cgtagacggt gtgttgccca tttgtattgg aaaagccaca
    18181 aaagttatcg aatatttgac agattctggg aaaatctatg taggcgagat aactctaggt
    18241 ttcagtacaa cgacggaaga tcgatccgga gaacgggtag aaacaaaacc agtagatatg
    18301 tctttatctg aaaaaaaaat agatgaagtg atggcttcat ttattggtga gatcacccag
    18361 ataccaccaa tgtactcagc ggtaaaagtc aacggtaagc ggttatatga atatgcccgc
    18421 aataatgaaa cagtggagcg tcctgttaga aaggctcaga tttaccgttt tatccggaca
    18481 agtgaattga caagagacga agtgacagga accatttctt ggcgcttcga agtagaatgc
    18541 gggaaaggaa cctatgtccg cacattagct gtagatacag gcaagaaact tggttaccct
    18601 gcgcacatgt ctgatctgac ccgaacagca agtgccggta tgtcccagaa ggatgctctg
    18661 acattagaag aagtagctca agctgtgaaa aatgggacaa tcgatgattg tttgctgcca
    18721 atagaaaaag gagtggaacg gttcaaacga gtagatatta ctgatgaagt atggcagaaa
    18781 gtaaaaaacg gtatgagact tgattatact gtatttgcgc tcccagaaat gccgtcagaa
    18841 gaaattgctt tgttttataa aggaaaagtt gtcagtattt atcagccaaa cccgaaagaa
    18901 aaaaacaaat taaaacctag taaagtattg agaaatgagg aataacgaat gaatatcatc
    18961 aagatcagac atccttacca atcagaacaa attccagaag acgatgtcgt tttagtgctg
    19021 ggatttttcg acggcgtgca tatcggacac cagaaagtca taaagacagg tatggaaatt
    19081 gcacaaaaag aaggattgaa attagcattg atgactttca atcagcatcc atctattgtc
    19141 tttaaaaaga tcgatcctag cagcgtgaaa tatttgacta gccttaagca aaaagaagaa
    19201 aaaatggccg ctttgggtat agattattta tatgaagttg attttacttc ttcttttgct
    19261 catttagcac cgcaggaatt tgttgatcaa tacatcgtag ggttacatgc aaaatatgct
    19321 gtttccggtt ttgattatac gtatggacca aaagacattg cagatgtcgc tcattttcct
    19381 gaatatgcca aaggaagatt caaaatcgtc acagtttcta aagaagaaga acaaggtgaa
    19441 aaaatcagct ctacccgtat tcgtggatta ctagacagcg gacaagtaaa agaagcagca
    19501 aaactattag gttgcacata tgaggtagaa ggggtagttg tccatggcga tgcacgaggc
    19561 agactactag gattccctac agcaaatgtc aaagtaaaga gcacggttca tttgcctaaa
    19621 gaaggtgttt atgtgtcgga aataaaaatt ggggaaacat ggtatccagc catgggttca
    19681 attggacata atgatacatt cggtgaagga cgggagctga ctgtagagtt gtatattttg
    19741 gactttaatc aagatatcta tggtgaacat gtcgcggtac gctggaatca atttttaaga
    19801 gatcaagtga aattttctag tgtagaggaa ttgattgaac aactcaagga ggatgaacaa
    19861 gcaacagctg aatatttttc tcagcaataa aacattatgc ataaaatctc ttgtttatgt
    19921 ataactagca attacaataa aatgggtgat aaataagccc aggaaagggg ctgtgacaaa
    19981 agtcttgtca cagccttttt ttccaaataa atggtttttc agaagtagct tcttcggaaa
    20041 taagccgaaa tttcttgaag ctgaccactt ctgtcacaac ctctttcttt atagaaataa
    20101 aaggacacta gcaattcatg aaaacgattg ctagagcgta tcatttgctt tagactatat
    20161 atcttggtgt acactcactt taaggcggaa ataaaagtta tccgtaataa caggtttagg
    20221 tatgaaaaag gagtgggtac tatgtacaag gaattagaaa acaaagtcgc agtagtaact
    20281 ggtggctcaa aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat
    20341 gtagttgtca actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa
    20401 gaggcaggcg gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag
    20461 cgtttacttg atacggcttt agaaaatttt gggacactgg acgtttgggt taataatgct
    20521 gggatggaaa atcagcatcc aacccatgaa cttagccttg aagattggga aagagtgtta
    20581 aaagtcaatt tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag
    20641 aataataaaa aaggaaatat catcaatatg tcttctgttc atgaacagat cccatggcca
    20701 acgtttgctc attacgctgc ttcaaaaggc ggggtaaaat tatttaccga aacggtagca
    20761 atggagtatg caccgaaggg aatcagaatc aatagtatag gccctggtgc tatccgtaca
    20821 cctattaatg cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc
    20881 ccactaggac gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca
    20941 gacgaatcca gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat
    21001 ccttctttcc aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg
    21061 atcccggcag ttggctgggg gatccagcca ctgattttga aaaagattgg tggcagacca
    21121 acaaatgaga tattaggaac tggtattgga gcggtgcttg tcggcttact cgttcaatta
    21181 ttcgcttctc cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca
    21241 ttttgggtta ttggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa
    21301 acgatgccaa tttcgactgc attacagtta gtcggaacct cgttgattgg tgtcttcgct
    21361 tttggagaat ggtctggaag tatggggaaa atcattggag cagcagcaat cattcttctt
    21421 gtatttggtt ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca
    21481 aaaggaatta gtctattagc atcaacaagt attggttatt gggtatacag tgcattgcct
    21541 aaactagttg atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta
    21601 ggggcagtga tttatgtgat tttccgtaaa ccaaccgcat tttctgaaga caagagttgg
    21661 aaagcaacga tcgttggagc catctttagt gtgtcagcat tggcttatat tttttcagca
    21721 aatgaaaatg gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca
    21781 ttaggcggta tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa ttaacgatta
    21841 tcggattatt gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat
    21901 ggtaaaattt catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa
    21961 aaatagcgct agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat
    22021 acaagggaaa tatctgtctg gatcaataat ttttcattga tccattgact aaagtttacg
    22081 acataattga agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat
    22141 tgtaaaagac ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct
    22201 cggaattgat tgattgctgt aaaatcacga tattgaaggt tgaaacgcat cgtgattggt
    22261 gcatttagca tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg
    22321 aactcaaaaa gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt
    22381 tttccttcat gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat
    22441 tcatttactg agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact
    22501 tagttgatat ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta
    22561 aaaatcaagt cagaaaattc taacttttat ttctctaaag aaattcgttc tctcctaaag
    22621 tgatattata tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat
    22681 tttctcgtat tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt
    22741 actaagtaga aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc
    22801 ttataaaaat agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata
    22861 ggattgaaca aaacctgtat aaggtgatga atagtttcct cgccagtaaa aggtttattc
    22921 gtataaagct ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa
    22981 tcaattgttc aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat
    23041 ccgtatatac gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt
    23101 ccggtgcgat agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt
    23161 cagtaaacat aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta
    23221 tcttagcaaa aaggtctttg atcttttgtt gatgttcttt ttctttgatt tgattgatga
    23281 tcggctgaaa tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag
    23341 aaaaacaata gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat
    23401 ttatatgata agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg
    23461 gcaaagaaat tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat
    23521 acaaatgaag atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa
    23581 agcttattct taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa
    23641 caaacggatt tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca
    23701 gaacgcatgg aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa
    23761 aaacagattc cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca
    23821 ccaggtagac cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag
    23881 ctacttgaga tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa
    23941 atagtgcata cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca
    24001 acgattgggg ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa
    24061 tcagctttaa agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg
    24121 acgcaagata aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg
    24181 atgcaagatg aaaatatcca aatgatcttt ccgacttggg gaggagaatt attgattgag
    24241 attctcgatt ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat
    24301 attagtacgt tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaact
    24361 aaccttattg atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta
    24421 ctgaaaacaa gagaaggaga atcgattcta cagcaatctt ctgaaaagta tcaaaaagtg
    24481 tggccccatg agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa
    24541 tcagtcagcg gagatgcagt aaggatgaag ggacgattat taggtggttg tattgatacg
    24601 atcaggcatt taatcggtac tccttatgga gatgtccaat cttttaaaag aaagtataat
    24661 gatggaaagc cgatcttgtg gtttctagaa aactgtgagc tatcgtcagt tgatttaaga
    24721 agatcacttg ttcagatgaa actggcaggc tggtttgatg gcagctcggg gatcatgttc
    24781 ggtagaagtg cggcagagga tttgttagat tataaaatga aggatgtata cgaagatctg
    24841 tcaaaggaat taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa
    24901 ctgacattga ttaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt
    24961 ttgcaaacgt ttatttaaaa aattttttcc tgccttggag tctatcaaag accaataaat
    25021 ctaaatagaa aagaaagtga aagaagctga caaaatgctg tcagcttctt tttgtataac
    25081 atattagaaa gtctaattat ctataactgt cacatcgatt aactgtttca cgtcattggg
    25141 atttcctgta ggaatagcta ctacagatgc attatcaatg atttcttgag ttgtttctct
    25201 aatgaacgct ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata
    25261 actagtgatt gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt
    25321 cccagtgagc ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat
    25381 aggtgttcct gcaggataat gaataacatg taattcgaaa tcacccgatg tatctctaaa
    25441 agtattacct ctaatagcta gccaaacacg tgcgtactca atttgtagat cagaatatcc
    25501 tgctaacgga tctattgact gagaagactg tgaggaggtt gtgtctattg ttgaagaaga
    25561 aatatttgtc tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact
    25621 gcttgttgaa gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc
    25681 tgaactggtt tgtctgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt
    25741 actaggagac gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt
    25801 tttcatataa aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg
    25861 aatccgtaaa accttgtcta tgaatataaa tagagtgtat cttctttgat aaatgtcaga
    25921 aaaagtgtga caaataatca tcagtaaaat tacttgtcac actattgata aggagtattt
    25981 aattgaagag aatgattagg agaaaggatc tccgtacttt ttatagtaat catagaatcc
    26041 aagtttgtat agacgaatcg ttttttctct tatttcttct gtttcaaaat ggactgcttg
    26101 aagaatcaag tctgtgaatt ctataggagc tgttccattc gcagtaatca aattacgatc
    26161 tttcacagct tgttgttcta taaaatcttc tttattctga taattttccg attctaacca
    26221 aggaaattgc gcatttccgg tgtgtttgta accttgcaat aatccgctct gagctaaata
    26281 atcaactgcc ccacaaatag cagctactgg tacgtctgtt tttaacaatt tatcgattat
    26341 ttgatataaa tgatgatttt ttatgtccca tgaattgcca ccgatcataa taaaaaggtc
    26401 aactttttca ggtatttgtt cgattaagta atcgattttt gtttttaatc ctccaatgga
    26461 agtgacctct gattggatag aagcagtttt gttttgccat ttctcatttt ggtttagtgt
    26521 gctagtaata taagcacctt cccaatcggc atattcctct aatagaaacg taatcgcagt
    26581 tttcattttc aagctctcct ttactatcta taacaaggac aacagtttta acctacagac
    26641 attcattaaa attgggtagc gacagcgaaa acaacaattc ttaagtttta gacagagtat
    26701 acagatagtc aacttaatta aatacgaact aaataaaaat gacaggtaat catttttgtt
    26761 taccagcatt ataacatttt gcatacatgg atgacaaaag aaccaattcc cacttgcttg
    26821 agtacaatat taaaagaaac tggaaggcaa aaagtaatta tagaaacgaa aaataaatca
    26881 tatcgggcac tcagacatga aaacaaccct agagatatat aactaggtaa ccagtttaat
    26941 gaaagaaaaa atcatccaag aagtcgattc ttggattttt tagtggattt gttcgccaaa
    27001 tacgtttagt atgcgtatat gagaggttgt aagcaaagcg ttgtggtctg agcacttaag
    27061 gaggaatctg tgaaaatagc ttttcatatt tttgcagatt ttgacttaat gccgaaggtc
    27121 agctttgcga ttaccgttta tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat
    27181 aagcaagcat ttttagagct aggtttcctt gattctaaat atgcggctta tttcaatgag
    27241 ttggtgcttg aacgcgattt taagaaggtc gaaaacaggc acgacttttc gacgtaagga
    27301 gaaagaattt caatgataga aacaataaat cccgcagaaa acaaggtttc tgcgtacatt
    27361 catatcccat tttgcgagca tatctgctat tactgtgatt ttaataaagt ttttttagaa
    27421 gggcagccag tagatgagta tgtggaaatg ctgctgaatg aaatgaaaat aatgctagaa
    27481 agtcatccga cagcgaattt agaaacactt tacgtagggg gcgggacacc aacttcttta
    27541 tctgctaaac aacttgatcg actgttatca ggtgctagag agatcctgcc ttttcaaaat
    27601 ggtaatgagt ttactgtaga agcgaatcca ggtgatttga cccgtgaaaa acttcaagta
    27661 atgaaaaact atggcgtcaa tcgtttatcc atgggagtgc agacatttga taatcgctta
    27721 ttgaaaaaaa ttggacgtaa acacaccgca gaagatgtgt atcaaactat gcaattttta
    27781 gaagcagaaa attttaccaa tgtaagcatc gatttgattt atgcattacc gggacaaaca
    27841 ctagaaggat atctggatac tttggaaaaa gctttggctt tggatctgcc tcactattct
    27901 ttgtactcat tgatcctcga aaacaaaaca atgttcatga actgggtacg acaaggaaga
    27961 ctcgaactgc ctgatcagga aacggaaacc agaatgtttg aagagacgat tgaagcaatg
    28021 gaaaagcatg gacgtcacca atatgaaata agtaactttg ggctctccgg acatgaatct
    28081 caacataacc tcatgtactg gaacaatgac cactattttg ggtttggagc tggtgctagc
    28141 ggttatcttg gagatattcg ttaccgcaac aaaggaccaa tccaacacta tttacgccca
    28201 ttacgagcaa atgaacgccc ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata
    28261 gaagaagaaa tgttcttagg actaagaaaa aaaacaggga ttactaaaaa acatttctat
    28321 gatcgctatc aaaaaacaat tgaatcggtg tacggtgatg tgctaaggga attagagcaa
    28381 gaagggttac ttgttaatga agaagaccgt gtttatttga caccaagagg gacctttttg
    28441 gggaacaatg tgtttgagcg ctttttgttt gaaaaatagt ttaaagaata tttgtttttt
    28501 atctgaattt aaataaaaaa agttcaaaaa tacaataaag gactattgaa acgttgatat
    28561 aactggattt acatcaatta tttagaatta gcactcttgt gtttagagtg ctaaaaaatg
    28621 gagtctttct cttgacagga gaagggcttc ttgctatatt tataagtgga ttagcactta
    28681 gaatatatga gtgctaatga gaggtgagga agatgttaac aaagcgacaa aacgacattt
    28741 tacgtctgat catccaacac tatacgagta gtggcgtgcc cgttggttcc aaaacattga
    28801 tgtctgaggg agtcaaggcc agctcagcaa cgattcgcaa tgatatgaaa gcattagaag
    28861 atgaaggatt gctgttgaag actcactctt cttctgggcg gattccatct gctttaggct
    28921 atcgttatta cgtggatcat ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc
    28981 tgattcgtca gtctctaggc agagaatttc atgaaatcaa tgagattatt aagcaatctg
    29041 ctgagatatt atctgagctg acaagctata cgactttttc attgggacct gaaataaaag
    29101 accgccgact gactggcttt cgtatcgtac ccttgaataa tcgacagatt attgctatcg
    29161 tcgtaacaga tatggggaat gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa
    29221 gtcaggaact agaaaaaatg gtacagattg taaacgaccg tctagtcgga gatcctctga
    29281 tgacggtgta ccataaactt cgtacggaga ttccgatgat tcttcataaa tatttccaaa
    29341 cgacagaagg gatgtctcag ttatttgaca ccgttcttgg tcatgttttt gaagataagg
    29401 tttatgtcag tggacaaatg aatctcttgg attatgagcc ccgtcaagat cttgatcagt
    29461 tcaaatcgat gttttctttt atgaaagact cagacggact tacacaaatg atcactccga
    29521 tggatagcaa tattcatatc aagattggtg ctgaattagg caatgattta ttgcagaata
    29581 tgagtatgat tcaagcaagt tacgagatag atggtcatgg aagaggtaca atagccttgt
    29641 tagggcctac tagtatgcct tattctcgga tgttcggtct agtcgatgtt tatcgaagag
    29701 aactagcagc taaacttgct gattactacc gttcgcttga cttgtcaaac tcgtaatgtg
    29761 aataacgaag ctatttgcag ctgatcgttc tctctttctt agtaagagca atcaaacagc
    29821 tgactgaata atgaaaggaa gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag
    29881 aaatgacaga tgctcagcca gaaccggaaa tcgatgtcga agctgcagaa gattcgggta
    29941 tttcagaagc tgaagcggaa gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa
    30001 tggaagataa atacttgcgg gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa
    30061 atgagcgtga gcaattacaa aaataccgtt cacaagattt agccaaaaaa ttacttccat
    30121 caattgacaa cctggaacgt gcattagcca cagaagtgtc agatgatcaa ggggcaggtt
    30181 tgaaaaaagg cgtggaaatg gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa
    30241 ttgaaaaaat ccctgctaaa ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa
    30301 ctgttccagc gactgaagat acaccagccg atacgattgt agaagtacta caagaaggct
    30361 acaaattaca tgatcgcgtg ttaagaccaa ccatggtcat tgttgcacaa taattgtgcg
    30421 gatataaaac ttacactttc aattaaagaa ggagacgtta attatgagta aaattatcgg
    30481 tattgactta ggtacgacta actctgcagt agcagtattg gaaggaaacg aagcaaaaat
    30541 catcgctaac ccagaaggaa accgtacaac accttctgtt gtatcattca aaaatggaga
    30601 aatccaagta ggggaagttg caaaacgtca agctgtgaca aacccaaata cgatttcatc
    30661 aatcaaacgc catatcggtg aagcaggtta caaagtagat gtagaaggta aatcatatac
    30721 accacaagaa atctcagcaa tgatccttca atatatcaaa ggattcgcag aagactattt
    30781 aggtgaaaaa gtcgaaaaag cagttatcac tgtgccagct tactttaatg atgcacaacg
    30841 tcaagcaaca aaagatgctg gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga
    30901 accaactgca gctgctcttg cctatggttt agataaaaca gatcgcgacg aaaaaatctt
    30961 agtattcgac cttggtggcg gtacttttga cgtatctatc cttgaactag gagacggtgt
    31021 ctttgatgta ttgtcaacag ctggcgacaa ccatctaggt ggggacgact ttgataacaa
    31081 aatcatcgac tacatggtag cagaattcaa aaaagaaaac agcattgact tgtctcaaga
    31141 caaaatggct ttacaacgct tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg
    31201 cgtatcaagc acacaaatca gcttgccatt tatcacagca ggagaatctg gaccattgca
    31261 tttggaaatg acattgacac gtgcaaaatt tgatgaatta acagctgact tagtagaacg
    31321 tacaaaagta ccagtacgtc aagcgctaaa agatgcaggt ttatcacaat cagaaatcga
    31381 cgaagttatt ttagtcggtg gatctactcg tatccctgca gttgtagaag cagtacgtaa
    31441 agaaacagga aaagaaccaa acaaatcagt taaccctgat gaagtagttg ctatgggtgc
    31501 tgctatccaa ggtggcgtga tcactggtga tgtgaaagat gttgttttac ttgacgttac
    31561 accattgtca ttaggtatcg aaacaatggg tggagtgttc actaaattga ttgaccgcaa
    31621 tacaacgatt ccaacaagta aatcacaagt attctcaact gcggcagata atcaaccagc
    31681 agtagacatc catgtattac aaggtgaacg cccaatggct gctgacaaca aaactctagg
    31741 aagattccaa ttgacagata ttccagcagc tcctcgtggt attccacaaa tcgaagtaac
    31801 attcgatatt gataaaaacg ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa
    31861 agaacaaaaa atcacgatca aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat
    31921 ggtaaaagat gcagaagcaa atgcagaagc agataaagct cgtaaagagg aagttgacct
    31981 acgtaatgat gtagatgcat tgctattctc tgtggacaaa acattgaaag aattagaagg
    32041 caaagtggac gaagaagaag tgaaaaaagc agaaacagct cgcgatgaat tgaaagccgc
    32101 tgttgaagca aacaacatcg aagaaatgaa aactaaacgc gatgcattga acgaaatcgt
    32161 tcaaaacttg actgttaaac tatacgaaca agcggcacaa caacaagctc aagaaaaccc
    32221 agaagcagct caaggcggag cagatgatgt cgttgatgct gattttgaag aagtcgatgg
    32281 cgacgataaa taatcagttc actgaatttc gagagaaggc caaagttgct actttggctt
    32341 tttcttttga aaaatagttt gaagtatcgg tttttgtttc aaacaaaata tgatatgata
    32401 acaagcgacg tgaaaatgat gagaaccaat aggaggaaag cctatggcaa caaaacgtga
    32461 ttattatgaa gtcttagggc tctcaaaagg agcatcagaa gatgaaataa agaaagctta
    32521 tcgtaagctt tcgaaaaaat accatcctga catcaataaa gaacctgatg cggaagaaaa
    32581 attcaaagaa gtatcagaag catatgagat tttaagtgac ccgcaaaaac gcgctgcata
    32641 tgaccaatac gggcatgcag gcacagatcc aaattacggt gctggtggag ctggtggcgg
    32701 atttggtgga ttcggcggct tctctggtgg cggcttcggt ggttttgaag atatcttcga
    32761 ctctttcttt ggaggaggcg gtcggtcagt cgatccaaat gcgcctcgtc aaggtgctga
    32821 tttgcaatat acggtcgact tgtcttttga agaagcgatt tttggtgtag aaaaagaaat
    32881 caagtataat cgggaagaaa tctgtcatac ttgtggtggg aacggtgcaa aaccaggcac
    32941 acaaccaact acttgtcata aatgtcatgg tgcaggtacg attaatgtag aacgccaaac
    33001 accacttgga cgagtgatga gccgtcaaac ttgtgatgtt tgtcatggga cagggaaaga
    33061 aatcaaagaa ccttgtccaa cctgtcatgg ctcaggacat gagaagaaag cacattcagt
    33121 taaagtgaat gtaccagctg gtgtagaaga aggacaacaa atgcgtttag ctggtcaagg
    33181 agaagctgga gaaaacggcg gaccatttgg tgatttgtat gtggtcttcc gtgtagaaga
    33241 aagtgatatc tttgatcgtg aaggttctga aatttactat gaattgcctt taagttttgt
    33301 acaagcagct ttgggggatg aagtccaagt accaactgtc catggagatg tgaaattgaa
    33361 gatccctgct ggaacacaga caggtacgaa cttccgttta cgtggaaaag gcgctccgaa
    33421 gttacgtggt ggtgcaacag gcgaccaaca tgtcaaagta aaactgatca caccgaaaaa
    33481 tctaaatgaa caacaaagag aagcattacg tgcattcgct gaagctggcg gcatcaaagt
    33541 tgaagaacag caagaagacg gtttcttcga taagtttaaa gatgcttttg gcgggaaaaa
    33601 gcggaagtag tttttctagt aaaaacgcta agtcattaat aattaaaaac cagacgaaca
    33661 atcatatgtt cgcctggttt ttttgtagtt gtcatgtttg taccaacaaa aaagccagct
    33721 ttagaatatg ataatcgtca tagtttctag gggtataacc agtgagagaa tggaacttgt
    33781 tcaatcgata ttgcaaggtg tttttatgaa taaataattc ttctgagcaa tttttgatgc
    33841 ttccgttatt tttactgaag catgccaaag tcttcttgaa caaatgtact tcttcttctg
    33901 tcaggctttt caaaatacgt tttacatata atgttcgttg atctgacggg attgctggta
    33961 aaacgatccc gaggtctaaa tcttcaaaaa agcggatctc gttcacagat tgatggaaag
    34021 ctatccattc gcaaacttgt ttggcatgaa ggaaagattg cggtgtctct tgctctgtat
    34081 aacaaagcga cccaattcca aagtttagct gtaactgata cattttatta aactttgtct
    34141 ggattttatc aagcaatgat agcaatgagg ctctggaaat ccgattgact aggataatga
    34201 tttgttgtct atgtacagtg agcaaagcct gtccgtaata agagaagacc ctttcgagca
    34261 actccaataa aggattgatc cgttcttgac acaattgctg attgtcagaa agttgtccca
    34321 caactacata atatggagaa gaaaaatcaa gctcaattaa gtgagggtgg tatcgactat
    34381 gtacttgata tcatcaatga accgatggta ctttcttatg cgatggaaca ggtagagcga
    34441 ttgcttgttt gtgcaggaaa acaagcgatg cggctgatac gattaggtga gaaaataaga
    34501 agataaaaat acacggtccc ttcatggcga ccgtgcattt ttggttttta ttaaatattt
    34561 aatactttat tcaagaaatc ttttgtccgt tcattttgcg gattttcaaa gatttgttga
    34621 ggcgttccat cttctaaaaa gtttccgtcg tcaatgaaca tgacgcgatt ggccacttct
    34681 ttagcgaacc ccatttcgtg cgtaacgata accatcgtca taccttgttc tgctaacttt
    34741 ttcatgacgt tcaagacatc accgaccatt tcaggatcga gtgcagaagt aggctcatca
    34801 aaaagcatga tgtcagggtt catcgctaga gcacgagcaa tagccacacg ctgtttttgt
    34861 ccaccagaaa gcatttcagg atacatatct tttttatctt ctagaccaac tgtctgcagt
    34921 aatttgattg cttttttctc tgcttcttct ctagattctt tcttcaggtc catcggtgct
    34981 aaaatgatgt tttccaaaat agaaagatga gggaataggt taaagtgttg gaataccatt
    35041 ccgatatgtt gtctcaccaa gttgatatct gtgcttttat ccatcaagtg ggcgccgtca
    35101 ataataattt caccgtcaga aggttcttct aaacgattca agcaacgcaa gaatgtactt
    35161 tttcctgaac ctgaagggcc aatgatacag acgacatctc cttcgttgat ggatacattg
    35221 atgtcgttta atactgtgtt atcgccgtat tttttgacta aatgctcaac taagattttt
    35281 tctgccatct tacttcacct tcttttctaa aacttttgct aattttgtca atgctgtgat
    35341 caaaatcaag tacatgatcg caatgatcaa gtaaacatac gtactttgtg ttgtacgtgc
    35401 aacaatgatc ttaccagttt gcagcaattc gactacaccg attgcggaaa tgatcgttgt
    35461 gtcttttaat gagataacga actgattgac gaatgatggg atcatgatgc gtatcgcttg
    35521 cggcaaaatg attttttgca ttgtccgatt ataagaaaga cctaaacttc tggaagcttc
    35581 catttgtccg acaggaacgg cgttgatccc gcctcgaaca atctctgcga tataagcact
    35641 tgcgttaaga gtcaaggtga taacaccagc aaggaagtca ggaatcgtaa atccaacgat
    35701 accaggcaac ccaaagaaaa taaagaacgc aagaaccatc attggaattc ctcggatgat
    35761 atcgacataa atactagcaa ttgtgcgaag agcgcgtatt ggtgctacac tgaataatcc
    35821 aaaaatcaca ccgattacta tagccaaagc aaaggagatc aaagttagga gaattgttct
    35881 ccaaagtcct ttcaacaatg cttgatagtt gttttggata agtccaagaa atgttgattc
    35941 atcagcagtg gaagactcac tggaagtttc tgaaccatcc gctacatatt ctttaataat
    36001 cttatcgtat tcgcctgttc tttttaattc tttcaatcct tcattgaaca tattaagaag
    36061 ctcaggattt tgtccttttt taacggcaaa tgcatatttt ccgccagatt cttttttgat
    36121 cggtgtttcc aattcttgtc cttgtttgat cgcataaccg attaccggat aatcatccat
    36181 cattgcatcg attgcgccaa cttttaaccc gtcgtacaat tgatcagcag catcaaaata
    36241 tttgatagaa tagccatatt cagattcatg gtcgcttaaa aattcagcac tttcggtacc
    36301 aacttttgct ccgactgttt ttcctttcaa atcttcataa gacttgatcg aagtatctcc
    36361 ttttttgaca gctaattgga tcccgctatc aaagtaagaa tcagaaaaat catagctttt
    36421 ctttcgttca tctgttacag tcattccggc aatcattccg tctgcttgtc ctgattccaa
    36481 agcttgcaaa gcagcagaga aaccacggta gtccattcgg atgttaaatc cttgcagttt
    36541 ggcgatgttt tccatcaatt ttacatcgat gccttcgtac tcgccctttg cgttttgaaa
    36601 ttcaaatgga gcaaaagcag agtcactggc aattacgtat acgtcttttt tgggctctat
    36661 tttttgcatt gtctcgcttg tgcttgactc tgccgttgta gtgttgtcag cttgtgcaac
    36721 atgaccaaac gcaagtgtca caagtcctgt cattaagaaa acaaatgagt gaaataaatg
    36781 ctttcttttc ataatcaacc tcctaaattt ccaatatttt attattatag gcgaaaagtg
    36841 ctgaatcaga caagactgta agctcgaaaa aatgaaaaaa atgcgagttt ctaacatttt
    36901 atgttataaa acataacatg tataataaga taaaaaagtg gcgaaagtat gttcggtgtg
    36961 gtataatttt ttagtaagga atgcctttgc tttccttttc tttttattgg tcgtaaaatg
    37021 aaaaggaatg aatgaaaagg aggacgaaga atgattgaga gagatacctc ccgacacttt
    37081 gagcttgtct cgaaatacca accggctggt gatcagcctg aagcgattaa ccaattagtg
    37141 gatggcgtag tcggcgggaa aaaagcacaa atcttattag gtgcgactgg aacagggaaa
    37201 acatatacga tttccaatct tattgagaaa gtgaataaac caacactgat tattgcccat
    37261 aacaaaacat tggctggtca gctgtacggg gaattcaagg aatttttccc taacaatgct
    37321 gttgaatatt ttgtttctta ttatgattat tatcagcctg aagcttatgt gccttcaagc
    37381 gatacgtata tcgaaaaaga ttcaagtgta aatgatgaga tcgacaaatt acgacattcg
    37441 gctactagtt cattattaga aagaaatgat gtgatcgtca ttgcatccgt ttcatgtatt
    37501 tttggattag gctcgccttt tgagtaccaa aaacaagtag tttcgattcg acagggagca
    37561 gaactagatc gcaatcagtt gattcgtgac ttagtcagta tccaattcga aagaaatgat
    37621 atagattttc agcgcggtcg ttttcgagtg cgcggtgatg tcgtggagat ttttccggct
    37681 tcaagagatg aacgtgcctt acgtgtggag ttttttggag atgaggtcga acgtatccga
    37741 gaagtcaatg cactgacagg tgaagtttta ggagaaacgg aacacgtagc aatcttcccg
    37801 gcgactcact ttgtgacgaa tgatgaacat atggagcatg ccgtagcgaa tatcaaagcg
    37861 gaattagaac agcgtttaac tgtcttgcga aatgaaaaca aacttctgga agctcaacgt
    37921 ttagaacagc gaaccaatta cgatattgaa atgatgttag aaatgggcta cacgtcggga
    37981 attgaaaatt attctcggca tatggacgga cgtaaagaag gtgaaccgcc ttatacgcta
    38041 ttggatttct ttcctgaaga ttttttgatc gtggcggacg aatcgcacgt aaccatgcct
    38101 caaatcagag gaatgtataa tggtgaccga gcgagaaaac agatgctagt agattacggt
    38161 ttccgtttgc catctgcttt agacaatcgg ccacttcggt tagaagagtt tgaaaaacat
    38221 gtcaatcaga tcatttatgt ctctgcgacg ccaggaccat atgaacatga acaaacagat
    38281 accgttatcc aacaaatcat ccgtccgaca gggctgcttg acccagtgat cgaagttcgt
    38341 ccaatcatgg gacaaatcga tgatctagta ggcgaaataa atgaacgagt cgaaaaagac
    38401 cagcgggtgt ttgtaacgac attgacgaaa aaaatggcag aagacctcac agattatttc
    38461 aaagaattag ggatcaaagt caaatatttg catagtgata tcaaaacgct ggaacgaaca
    38521 gaaatcattc gagatcttcg attaggtgaa tttgatgtgt tagttggaat caatttgctt
    38581 cgtgaaggat tagacgtacc tgaagtttcc cttgttgcta tcttggatgc ggataaagaa
    38641 ggctttttac gtagtgaacg ttccttagtc caaacgattg gacgagctgc tcggaatgaa
    38701 gaaggtaaag taatcatgta cgcagacaaa gtaacagatt ctatgcgtct agcgatggat
    38761 gaaacgtcaa gacgtcgtac gattcagcag aagtacaatg aagaacatgg gatcgttcca
    38821 aaaacgatca ttaaagaaat ccgagatttg atttctatta cgaaagaatc ggaagatgat
    38881 acaaaagaag cggtacaagt ctcgtacgaa gaaatgacga aggaagaaaa agacacgtta
    38941 ttgatgaaat tagaaaaaga aatgaaagat gcggcaaaag ctttggattt tgaaacagcc
    39001 gcgaatgtgc gagatatgat tttagaatta aaagcttcaa agtaggagga caacatggct
    39061 aacgataaaa ttacgattca tggtgcacgt gcccataact tgaaaaatat agatgtaacg
    39121 attccacgag ataaattcgt ggtcgttaca ggtttgtccg gttctggaaa aagttcccta
    39181 gcatttgata cgctgtacgc tgaagggcag cggagatacg tagagagcct ttctgcttat
    39241 gctcgtcagt ttttaggaca aatggataag ccggacgtag atagcatcga cggattgagt
    39301 ccggcgattt ctattgatca gaaaacaaca agtaaaaatc ctcggtcgac agtaggtact
    39361 gtgacggaaa tcaatgacta tttacgcttg ttgtttgcac gtgtaggaca tccgatttgt
    39421 cccaacgatc atatcgagat taccagtcaa tcagtagatc aaatggcaga tcaagtaatg
    39481 gctttgcctg agcgcacaaa gatccaagtg ttggcaccga tcgtggtaaa gaaaaaagga
    39541 caacataaaa aaatattcga acgaatccaa aaagagggat acgtccgcgt tcgagtcgat
    39601 ggtgagatct atgatttaag tgaagtgcct gagctggaga aaaacaaaaa acatgatatc
    39661 gcgatcgtca tcgaccggat cattgtgaaa gaagggattc gttctcgcct atttgattct
    39721 ttagaggcgg ccctgcgatt agcagacggc tatgctctgg ttgacgtgat cggtgaagaa
    39781 gaaatgttgt ttagcgaaca ctatgcctgt ccttattgcg gatttactgt tggcgaacta
    39841 gaaccaagac tgttctcatt caatgcgcct tttggagctt gtccagactg tgatggttta
    39901 ggtgtcaaat tagaagtcga tcaagattta gtgattccag atagaacgaa gacacttagt
    39961 caaggtgcta tcgttccatg gaatccaatc agctctcagt attatcctca aatgttagcg
    40021 caggcttgtg aaagtttcgg tatcgattta gatacaccat tcgaagaatt accggaagag
    40081 catcaggaga tcatcttgaa tgggacagat gatcttttcc atttccatta tgaaaatgat
    40141 tttggtggcg ttcgcgatgt agaaacgact ttcgaagggg tattgaagaa tatcgagcgt
    40201 cgttatcatg atacgaacag tgatttcacg cgtgagcaaa tgcgtcttta tatgacagaa
    40261 ttaacttgtc agacatgtaa aggttatcga ttgaatcccc aagctctatc tgttcaaatc
    40321 aatggaacga atatcgggca aacaaatgag ttgtctattc aaaaagcagt agatttcttc
    40381 tcaaacttaa cgttatctga acaagagaaa gtaattgcca aaccgatttt aaaagaagta
    40441 aatgacagac tttcattctt ggaaaatgtg ggactagact atctaacttt gagtcgcgct
    40501 tccggaacat tatctggggg agaagcgcag cgaatcagat tggctacaca aattggatcg
    40561 aatctttctg gagttttgta tattttagat gaaccatcga tcggattgca tcaacgcgac
    40621 aacgatcgac tgattggctc attaaagaaa atgcgtgatc ttggcaacac cctcgttgta
    40681 gtcgaacatg atgaagatac gatgtgggca gcagattatt tgattgacgt cggacctggg
    40741 gccggagaac aaggcggaca aatcgtggct gcaggcactc ctgaagaagt agctaatgat
    40801 gaaaattctt tgacagggaa atatctttca gggaaaaaat caatccctgt accaaaagaa
    40861 cgccgtaaag gtaatgggaa agcaatcaag atcactggtg cttcagaaaa caatctgaaa
    40921 aatattagtg ttgaatttcc attaggagaa tttgtagcag tcacaggtgt gtctggttct
    40981 ggaaagagta cattggtcaa tagtatctta aagaaatcgt tagcgcaaaa attaaataag
    41041 aattctgcta agccaggtaa attcaagaca atttccggct acgaaagtat cgaaaagatc
    41101 atcgatatcg atcaaagccc aatcggccgg acgccgagaa gtaatccagc gacttataca
    41161 agtgtatttg atgatatccg tgggttattt gctcaaacga acgaggcaaa aatgcggggt
    41221 tataagaaag ggcgttttag tttcaacgta aaaggcggtc gttgtgaagc ttgtcgcggg
    41281 gatggaatta ttaagatcga aatgcacttt ttgcctgatg tctatgttcc ttgtgaagta
    41341 tgtcatggta aacgatataa ttctgaaaca ttagaagtgc attacaaagg aaaaagcatt
    41401 gctgatattt tggaaatgac agtagaggat gctgtagaat tcttcaagca cattccaaag
    41461 attcatcgca aactgcaaac gattgttgat gttggcttag gttatgtgac gatggggcaa
    41521 ccagcaacga cattgtccgg tggtgaggca caacggatga aacttgccag tgaattgcac
    41581 aaaatctcta atggaaagaa tttctatata ctagatgaac caacgacagg acttcatagc
    41641 gatgacatcg cccgcttgtt gcatgtatta caaagattag tagatgctgg taacacagtt
    41701 ttagtgattg aacacaatct agatgtaatc aaaacagcag attatatcat tgatttagga
    41761 ccagaaggtg gagaaggtgg aggaacgatc cttgcgactg gaacaccaga agaaatcatt
    41821 aacgtaaaag aaagttatac aggtcactat ttgaaaaaaa taatggtata aagaggatag
    41881 atagctgaga tagttaaaaa aacataaaaa aatgaatttt tgcttgtttt attaatatca
    41941 gtgttctaga atcattacat ggtagttctt tctacactga ttggtataga taaaaacttc
    42001 ctataagttc aatccgttca aaaaagaatt gagcaatcgg aggactttcc tttgattgct
    42061 tttttcttta tggtatgata ttccttgtgt agttggaaaa gaaaaacaag agattatgag
    42121 taagtgagag ataatgatga acagtattga aaatgaagaa tttatcccta tattattagg
    42181 aagcgacatg aacgtttatg gtatggcacg ttcttttaat gaagcatacg gcaaaatctg
    42241 ccaagcatat gcttcggatc agttagcccc tacccgctac agtaaaattg tcaatgtaga
    42301 agttattcca ggatttgata aagaccctgt atttatcgag acgatgcttc gtttggcaaa
    42361 agaaagatat tctgataaga gtaaaaaata tcttttgatt gcttgtggtg atggatatgc
    42421 agaattgatc tcccaacata aacaagagct gtcagaatat tttatctgtc catatattga
    42481 ttattcttta tttgaaaggc tgatcaataa agtaagcttt tatgaagttt gtgaagaata
    42541 tgatcttccg tatcctaaaa cattgatcgt cagagaagaa atgttagtaa atggacattt
    42601 ggaacaggaa ttgccttttg aattcccagt tgctttgaaa cctgcaaaca gtgtggagta
    42661 tctttctgtc caatttgaag gaagaaaaaa agcttttatt ttagagacta gagaagagtt
    42721 cgatttaatt ttaggccgga tatacgaagc cggttataaa agtgaaatga ttgtacaaga
    42781 ttttatcccg ggtgatgata gtaatatgcg tgtgctaaat gcgtatgtag atgaagatca
    42841 tcaagttcgg atgatgtgcc ttggtcatcc actattagaa gatcctacac cagcatcgat
    42901 tggtaattac gttgtgatca tgccagatta taacgaaaaa atctatcaaa ccattaaggc
    42961 atttttggaa aaaatcgaat atacaggctt tgccaatttc gatatgaagt atgatcctag
    43021 agatggagaa tataaattat ttgaaatcaa tttgagacaa ggtcgtagca gtttctttgt
    43081 gacattgaac ggcttgaatc tggctcgttt tgtcacagaa gatcgagtgt tcaacaaacc
    43141 ctttgttgaa acgacctatg gaacgaatca atcagataaa gcaagattat ggatgggtgt
    43201 gcccaaaaaa atctttttag aatatgctcg ggaaaatgaa gacaaaaaac tagctgagca
    43261 aatgatcaaa gaaaatcgtt acggtactac tgtattttat gaaaaagacc gatcaatcaa
    43321 gcgctggttg ttgatgaaat atatgttcca taactatatt ccaagattca aaaagtattt
    43381 tcatgtgaaa gaaggctagt tcatggagaa ttttttcagt attttaggcg gaatgggcac
    43441 gatggcgaca gaaagttttg ttcgcttgat caaccaccga acgaaagcta caaaagatca
    43501 agaatatttg aattatgtct tattcaatca tgcaacagtt cctgatcgta cagcttatat
    43561 tttagatcga tcagaagaaa atccaatgcc gtttctgctg gatgatattg agaaacaaaa
    43621 tttattgcga ccaaacttca ttgtgttgac ttgtaacacc gcacactact tttttgaaga
    43681 gttacaagcg gcaacggata ttcctatctt gcatatgcca agagaagcag caaatgaact
    43741 tgtccgtcag catacgacag ggagagtagc gattctagga acagaaggaa gtatgaaagc
    43801 tggaatctat gaacgcgaag tcaaaaatct tggtttcgaa acagtgattc ctgatactgc
    43861 gcttcaagaa aaaatcaatt acctgatcta tcatgagatc aaggaatcgg attatttgaa
    43921 tcaagagctc tattacgaaa tcttagagga agcggtagag cgcctaaact gtgaaaaagt
    43981 catcctaggt tgcacagaat tatctttgat gcatgaattt gctgaagata atcattaccc
    44041 agtgatcgat gctcaatcta ttttagcgga tcgaacgatc gaacgtgcat tagctgaaag
    44101 aagcgaggca ttggatacag catcggaaaa gtaaaaaaat tggatttggg gcaaatatcc
    44161 taaaagatat ttggtccaaa tctttttgta aaagaaaaag aaaactggta atgatacgcc
    44221 cgggatgata taattttatt atgcacaatg tgagaggggt tcgtaaatat gacagataat
    44281 ttgcaattag tcatcataac aggtatgagt ggtgcaggga aaacagtagc tatccaaagt
    44341 tttgaagata tgggctattt ttgtatcgat aatatgccgc ctcgattgat tccaaaattc
    44401 tgggaattga tcaaagaatc aggtaaagtc acaaaaatgg ctttaggagt ggatttgcgt
    44461 tctcgttcct tctttgaaga aatccaaaac atgctgatcg aaatcgaaaa tacgaacttc
    44521 atcgacactt ctatcttatt tttagatgct tcagacgaag aactagtggc tcgctacaaa
    44581 gaaacaagac gagcacatcc aatggcgatg gacggtttga tcactgaagg gatacgtaaa
    44641 gaacgggcga tactagacga cttgaaagcc caagcttccg tgatcattga tacgacaaca
    44701 ttgacaccac gacaattacg tgaaaaaatc aatgagtcat tcaaatcaaa gggagaccaa
    44761 ggatttcgga tcgaatttgt atcatttggt tttaaatacg ggttgccgat cgattcggat
    44821 attgtgatgg atgtgcgttt tttgcccaac cctcattata tcccggaact aagaccattg
    44881 accggaaaag atcagccagt ctatgattat gtgatgtcct ttgatgaaac gcaatcattc
    44941 taccataaat ttttggatct tctggaaact gtattgcctg gatacattaa agaaggaaaa
    45001 agcagcttga cagttgcaat cggctgtaca ggcggtcaac accgttctgt ggcattgact
    45061 gaacgtttgg caaatgcttt agggaaaaac tataaagtca atgtgacaca tcgggacaag
    45121 gataaaagga aagagacggt gaaccggtcg tgaaaatgaa aacctaccgg ataagaaaac
    45181 caaagatcgt agtggtcggc ggtggcactg gtctgccagt tatcctgaaa agtttacgaa
    45241 atcaaggagc agatatcaca gcagtagtga ctgttgcaga tgatggtgga agcagcggtg
    45301 caatcagaga atcgatcgct atggctcctc ctggtgactt gagaaatgtc ttagtggcgt
    45361 tatcggatat gccacaattc tatgaagata ttttccaata ccgtttcaaa aaggaagatc
    45421 agtttttagc caatcatacg atcggtaatc tgatcattgc cgcagtttcc gagatgcggg
    45481 gaagcactta tgaggcgatt caattattag caaaaatgat gcatgtagat ggacatgttt
    45541 atccttcttc agaaactcct ttgaccttgc atgctgtttt taaagacgga acgactgcag
    45601 taggagaatc taaaatcgcg atcaaccgta aaaccattga ccgtgtgtat gtctgcaaca
    45661 caaatgataa aagtgaagcc aaagcggctc gaaaagtcgt ttcagcaatt atggacgccg
    45721 acatggtcgt tttgggaccc ggaagtttat tcaccagtat ccttccaaat ttagtgatac
    45781 cagaaatcgg cgaagcaatg ctcaagacaa ctgctgaaac cgtctatatc tgtaatatca
    45841 tgacccaaaa aggggaaacg gaacatttca cagatgccga tcatatacgt gttttacaca
    45901 agcatttgaa tcagcctttt atcgatacag tattggtcaa tacggaaaaa gtgcctgaag
    45961 attatatgga cccagagatc tatgatgagt atctagtaca agtcgcacac gacttcaacg
    46021 gattgagaaa tgaaggatgt cgagtgattt ccaccgactt tttagaattg aaagatggtg
    46081 gtgtcttcca cgatggagac aaagtagtag aagaattgtt ccgtttagtt ttcggttcaa
    46141 aatattaagc aatttattgt tggagaaagg aggagaatag gatgtcattt gctgcagatg
    46201 tgaagaaaga actaactggt ttggaagttc atcgagaaca tgcgaaagcg gaattagctg
    46261 ctttgctgcg aatgaacggc tctttaggta ttgtgaacca gcagtttatt ttgaacgtcc
    46321 aaacagaaaa tgctgcaatt gccagacgta tctattctct tttgaaagat cattatcagg
    46381 tacgaagtga gctactcgtt cgtaaaaaaa tgaaattaaa aaagaacaat gtctatatcg
    46441 tccggttgaa gcaaggcaca aaagatgtgt tgatggatct tgatatcatg gatggtatga
    46501 tgttcaatgg acacgtttcg gacgagatta tgggaaattc gcaaaaaatg cgttcttact
    46561 tacgtggtgc ttttatggcc tcaggttcag tcaataatcc ggaaacgagt cgttatcatt
    46621 tggaaatatt ttctatttat gaacagcata accaagacat ttgcgatatg ttaaattatt
    46681 acggtctaca tgctcgaacg cttgaacggc gcaatggcta tatttcgtat ttaaaaggag
    46741 cagaaaaaat tgctgatttc ttaacattga ttggtgcaac caactcaatg ctccgatttg
    46801 aagatgttcg aatcgtacgg gatatgagaa attcggtcaa tcgtctagtc aattgtgaga
    46861 cagcaaatct gaacaagacg attgatgcgg cttctaaaca aatcgaaaac atcgaattga
    46921 tcgaagcaaa ggtcggtcta catgcccttc ctgaaaaact tcaggaaatc gcagaacttc
    46981 ggcttcaaca tccagaggtc agcttgaaag aattagggga aatgattccc tcaggtgcaa
    47041 tatcaaaatc gggaattaat caccgaattc gcaaaataaa tgaatttgcg gaaaaactgc
    47101 gtgaagaagc agtttaatcc aaagaaaaaa ctgatacatg aagcaggaga tgcgaatgta
    47161 tcagtttttt ttaaaagcta tacttgcttt tcttccgtca aactataatt ccaactttac
    47221 tggagtaaca taatgttgaa aggcttctat tgttttttaa tacaatcgta taaaaagcgg
    47281 ggagacaact gaatgaagat tttagtttta tacataaact tttgcttaat atagaagtcc
    47341 tgatatagat aggatcaagt atttatctat aactggagat taattaaaaa ctggaaggaa
    47401 ctgctcgatc aaccttgaga actgtttttc ttacttctgg tcaagattct cgtggttggt
    47461 ggataaggat ggttgataag ttcattggat tagctagtcg aaaacaacca ggtgtaatta
    47521 atgtagaagt tttaacaagg ggatggagtg atttattcag tgataatcat aagttcataa
    47581 atagtaattg ggatgtatgg ttggtatacg ttatacctga acttgaacta tcaagatttg
    47641 gtttagaatt tgagaatcga tgtttataaa gggagggcaa aaaatgtgct tttttacaac
    47701 ttttactatc attatgattg cagtactttt tatttttcta gatatagcaa agcgaaatac
    47761 agcattttta ttatatcggg ttttacttcg agcaggtctc ataacattta tatcgatcgt
    47821 tggatttttt ctattcacag ttattgtatt tatttggaga actacagcac caccattgcc
    47881 agagattaca tatggagaat ttccgtttag tcttgaatat gaaatagatg aggaaagaat
    47941 tttgattgaa gatacggtga ttgcagaatt tataggaagt tttagaggga atgtgacaac
    48001 tcaacctaga cgaagttgga gtataaaact tttaaataat agtggagagt tgaaagcatt
    48061 tcattatgaa ttcttaataa tagaaagtga agatgtaaca atcactttaa gtagtggctc
    48121 tgctggatat tttatgaatg atggtggaag caagccacag ctcacagtcc gtgatcgtat
    48181 tgcaggtttt atgcattttt ctcactatga cttagaagaa gctcgtaaaa tacttgcaga
    48241 atatgggatc acattaatca attgggaata tgcgccaccg ataagaaatt ttttcaatga
    48301 ttattattag tcttataaaa aacagaatgt aaaatttatg tgcgcgagta tttagttttc
    48361 cattttttgt ttgatagata tatagcattg ttgaagcatg ggatagtggt gccaatttta
    48421 tcaatgaggt tggtcaggca gttaacataa ttggtgatgc tataaatcca tttaattgga
    48481 attggaggaa ttaggtgtgg aaacagtgag attatggata ttcatgaaaa attttcgttc
    48541 ggctttgaga tatgttattc tggaatcaaa atcaggttat tatctgattg acacagatca
    48601 ctatactttt tggggaggct acctttttcc gatttttaat tggataatcc cccataaggt
    48661 tatgaaaatt aacttgcctg cagaagaaat aaatagatta atactcaaca aagatttaca
    48721 aaggaaaaga gagaaagaac attctaatgt tgcttggata ggtccagcat ttgcagtagt
    48781 gacttcttca attgtcggtc ctatgctaga gagtatttat agtgattttt ctattggaat
    48841 acgttattta tttgttactt taagtatagc tgttcttttg tgcataaagg gttatgttag
    48901 caagaagaat aaagatattt taacactaat cggtgtaaac aatttatcta gtactaaaat
    48961 tatactgttt cctgaatcta taaaagaagg atcaaggttg ttgataattt ttataatgct
    49021 tatactgttt tctgttttat ttggggtgat gtttatag
//
LOCUS       DACEUF010000006        48979 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.007, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000006 DACEUF010000000
VERSION     DACEUF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.007"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(217..957)
                     /locus_tag="IXG20_000404"
     CDS             complement(217..957)
                     /locus_tag="IXG20_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608622.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(1087..2034)
                     /locus_tag="IXG20_000405"
     CDS             complement(1087..2034)
                     /locus_tag="IXG20_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1608623.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(2163..2927)
                     /locus_tag="IXG20_000406"
     CDS             complement(2163..2927)
                     /locus_tag="IXG20_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1608624.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(2931..3320)
                     /locus_tag="IXG20_000407"
     CDS             complement(2931..3320)
                     /locus_tag="IXG20_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608625.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3455..3997
                     /locus_tag="IXG20_000408"
     CDS             3455..3997
                     /locus_tag="IXG20_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ1608626.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            4145..4777
                     /locus_tag="IXG20_000409"
     CDS             4145..4777
                     /locus_tag="IXG20_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608627.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(4911..5036)
                     /locus_tag="IXG20_000410"
     CDS             complement(4911..5036)
                     /locus_tag="IXG20_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1608628.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5170..6063)
                     /locus_tag="IXG20_000411"
     CDS             complement(5170..6063)
                     /locus_tag="IXG20_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1608629.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6245..7171)
                     /locus_tag="IXG20_000412"
     CDS             complement(6245..7171)
                     /locus_tag="IXG20_000412"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1608630.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7256..8017)
                     /gene="pflA"
                     /locus_tag="IXG20_000413"
     CDS             complement(7256..8017)
                     /gene="pflA"
                     /locus_tag="IXG20_000413"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1608631.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8261..10492)
                     /gene="pflB"
                     /locus_tag="IXG20_000414"
     CDS             complement(8261..10492)
                     /gene="pflB"
                     /locus_tag="IXG20_000414"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1608632.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10769..13219)
                     /gene="parC"
                     /locus_tag="IXG20_000415"
     CDS             complement(10769..13219)
                     /gene="parC"
                     /locus_tag="IXG20_000415"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1608633.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13231..15276)
                     /gene="parE"
                     /locus_tag="IXG20_000416"
     CDS             complement(13231..15276)
                     /gene="parE"
                     /locus_tag="IXG20_000416"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1608634.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15442..15876)
                     /locus_tag="IXG20_000417"
     CDS             complement(15442..15876)
                     /locus_tag="IXG20_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1608635.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            16003..16641
                     /gene="plsY"
                     /locus_tag="IXG20_000418"
     CDS             16003..16641
                     /gene="plsY"
                     /locus_tag="IXG20_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ1608636.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16773..17648)
                     /locus_tag="IXG20_000419"
     CDS             complement(16773..17648)
                     /locus_tag="IXG20_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1608637.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17730..18521)
                     /gene="codY"
                     /locus_tag="IXG20_000420"
     CDS             complement(17730..18521)
                     /gene="codY"
                     /locus_tag="IXG20_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1608638.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18539..19939)
                     /gene="hslU"
                     /locus_tag="IXG20_000421"
     CDS             complement(18539..19939)
                     /gene="hslU"
                     /locus_tag="IXG20_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1608639.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19953..20501)
                     /gene="hslV"
                     /locus_tag="IXG20_000422"
     CDS             complement(19953..20501)
                     /gene="hslV"
                     /locus_tag="IXG20_000422"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1608640.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20538..21443)
                     /gene="xerC"
                     /locus_tag="IXG20_000423"
     CDS             complement(20538..21443)
                     /gene="xerC"
                     /locus_tag="IXG20_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1608641.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21641..23017)
                     /gene="trmFO"
                     /locus_tag="IXG20_000424"
     CDS             complement(21641..23017)
                     /gene="trmFO"
                     /locus_tag="IXG20_000424"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1608642.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(22986..25064)
                     /gene="topA"
                     /locus_tag="IXG20_000425"
     CDS             complement(22986..25064)
                     /gene="topA"
                     /locus_tag="IXG20_000425"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1608643.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25186..26043)
                     /gene="dprA"
                     /locus_tag="IXG20_000426"
     CDS             complement(25186..26043)
                     /gene="dprA"
                     /locus_tag="IXG20_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1608644.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(26099..26866)
                     /locus_tag="IXG20_000427"
     CDS             complement(26099..26866)
                     /locus_tag="IXG20_000427"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1608645.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26859..27719)
                     /gene="ylqF"
                     /locus_tag="IXG20_000428"
     CDS             complement(26859..27719)
                     /gene="ylqF"
                     /locus_tag="IXG20_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1608646.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            27979..28377
                     /locus_tag="IXG20_000429"
     CDS             27979..28377
                     /locus_tag="IXG20_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1608647.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            28614..29156
                     /locus_tag="IXG20_000430"
     CDS             28614..29156
                     /locus_tag="IXG20_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1608648.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(29239..29790)
                     /gene="lepB"
                     /locus_tag="IXG20_000431"
     CDS             complement(29239..29790)
                     /gene="lepB"
                     /locus_tag="IXG20_000431"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1608649.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(29942..30169)
                     /locus_tag="IXG20_000432"
     CDS             complement(29942..30169)
                     /locus_tag="IXG20_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1608650.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(30166..30684)
                     /gene="msrA"
                     /locus_tag="IXG20_000433"
     CDS             complement(30166..30684)
                     /gene="msrA"
                     /locus_tag="IXG20_000433"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1608651.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(30704..31417)
                     /locus_tag="IXG20_000434"
     CDS             complement(30704..31417)
                     /locus_tag="IXG20_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1608652.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(31420..32316)
                     /locus_tag="IXG20_000435"
     CDS             complement(31420..32316)
                     /locus_tag="IXG20_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1608653.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(32474..33316)
                     /locus_tag="IXG20_000436"
     CDS             complement(32474..33316)
                     /locus_tag="IXG20_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1608654.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            33448..34101
                     /locus_tag="IXG20_000437"
     CDS             33448..34101
                     /locus_tag="IXG20_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1608655.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(34164..34691)
                     /locus_tag="IXG20_000438"
     CDS             complement(34164..34691)
                     /locus_tag="IXG20_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1608656.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(34711..35658)
                     /locus_tag="IXG20_000439"
     CDS             complement(34711..35658)
                     /locus_tag="IXG20_000439"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1608657.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(35670..37556)
                     /locus_tag="IXG20_000440"
     CDS             complement(35670..37556)
                     /locus_tag="IXG20_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1608658.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(37716..39794)
                     /locus_tag="IXG20_000441"
     CDS             complement(37716..39794)
                     /locus_tag="IXG20_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1608659.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(39807..40082)
                     /locus_tag="IXG20_000442"
     CDS             complement(39807..40082)
                     /locus_tag="IXG20_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608660.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(40444..40899)
                     /locus_tag="IXG20_000443"
     CDS             complement(40444..40899)
                     /locus_tag="IXG20_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608661.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(41053..42261)
                     /locus_tag="IXG20_000444"
     CDS             complement(41053..42261)
                     /locus_tag="IXG20_000444"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1608662.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(42373..42705)
                     /locus_tag="IXG20_000445"
     CDS             complement(42373..42705)
                     /locus_tag="IXG20_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1608663.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            42853..43728
                     /locus_tag="IXG20_000446"
     CDS             42853..43728
                     /locus_tag="IXG20_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1608664.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(43810..44391)
                     /locus_tag="IXG20_000447"
     CDS             complement(43810..44391)
                     /locus_tag="IXG20_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1608665.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(44397..45656)
                     /locus_tag="IXG20_000448"
     CDS             complement(44397..45656)
                     /locus_tag="IXG20_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1608666.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(45658..46704)
                     /locus_tag="IXG20_000449"
     CDS             complement(45658..46704)
                     /locus_tag="IXG20_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608667.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(46939..47214)
                     /locus_tag="IXG20_000450"
     CDS             complement(46939..47214)
                     /locus_tag="IXG20_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ1608668.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(47457..48767)
                     /locus_tag="IXG20_000451"
     CDS             complement(47457..48767)
                     /locus_tag="IXG20_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ1608669.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
BASE COUNT        13345 a        10248 c         8359 g        17027 t
ORIGIN      
        1 tcttctttat aaaaaaataa actatttttg caaacgatct gcgaaaatag tttgtttttt
       61 tattttgcca ttattttctt acttattggg agaaaaaatt cgcttaaact caaaaaaatt
      121 tgaggatacg atataaactt tcggatagag tataaaataa ggaaacgccg cagcgtttcc
      181 tttccaactg tagccgaata taaatttgta tatcacttat ttaaaatagc tattttctcc
      241 caaagagttt ccttgatcat cgatcaatcc ttgtaatgag cgttctaaga taccatggct
      301 ttttttcgat tttttttctt ctgactgctt tgtttcctct aacgtttttg ttcgattgaa
      361 tgttcgtaca gacggctcac catttctttt ttcttgatac ggcgtcggtt ctgtatcgaa
      421 caacagataa gagtcatccg tttttttcat cgattcgatt aaatgatcct cagaaatttt
      481 gctttctcta gggtctggga taatcgatgc gggtacatat ttaggaacaa aataagaacg
      541 ctcccgcacc ttcgtgtcaa cagatgcgtt tcgtgtagta tgcttggact ctgtccttgt
      601 ttcactttgt ctgacagaac gagaggaatc accaaaaagt ttttttcgtc ctgtcgatgt
      661 gatttctgat tggtaatggc cggtataatc cggtaatttt cctttatgct tttccaaatt
      721 ttccttacgc ggagctgact tcaatccatt tgttgcacga cgcttgcgac ggattttctc
      781 ggtgttcgtg ttcgtgaaat gaaattcttt catctgttca aacggggact gattctgttc
      841 agtcaaaaga tcttcttgtc cgtcaaataa tactcgcttc ttgtcgttgg ttaattttac
      901 gcctaactca tcatcgtacg caggaaaacg gaaatgtttt cgtctgattt ctttcattca
      961 aaccatcctt ctcaaaaatg gaacttttct caataatagc acagattcat tgttttcagt
     1021 agcaatgcgc tgattatttt aagaaaatag cgatgaattt cataaatata tatatagtaa
     1081 taagacttaa ttcgttgtct ctctgataac tagttcagga aaaaaacgac gataaccttg
     1141 actcgcccct ttttcttcta ttttttttaa caacatttct ccacatactt cacccatacg
     1201 tataatatct tgcttgatcg ttgtcaattt aggtgaactg atttggtcta aaaatacccc
     1261 atcaaatcca atcacaccaa attcttcggg caagtgtcct ccagcattta taatccctct
     1321 tactgctcct agtgctagcc gatctgaact acatacaaaa caagttttat ctgtaaaatc
     1381 tgcccagttt tctgtcatat atctttctac ttctgtcgaa tgatttggaa accgcaagat
     1441 tttactagga agatcaactt ccttcatcac agataaataa ccagtttccc ttgaaatttc
     1501 aaaaggctcg tccacttcga taccgatata aataatcgtt tgataatctc tttctaaggc
     1561 atattgcgtt gcttttgctg taccatattc attatcgctg tctacataat catatccttc
     1621 agaattttcc ccaaacaaga caactggttt ttccaaacgg tcgatccaat cgaagtcttt
     1681 ttttctgaca cctgtgatga tgtatccatc acaagaaccg acgtcgaagg catcatttgt
     1741 aactaattgg agagaataat gcgctttacc gatccgttta gcgattccca tcagcaaatt
     1801 catgtaatag ggctcagttg tgtcgatttc ttccagtata aataatttga tgatctgcga
     1861 ccgattcgtg acaagtgctt ttgcagcaat atttggacgg taatccagtt cagccattgc
     1921 tgagaaaact aactctttca attcgtcagt cacctgttcc ggatgattga tgactcgaga
     1981 gaccgtcatt tttgaaacat tggcacgttt tgcaacatct gctaaagtgg gcatgctctc
     2041 ttcctttcta acatgttgtc attcttacta tcaattatac caaattttcc taacagagtt
     2101 tattatagcc caaaataaaa aacggaaaat cctctatcgt catgagtgat tttccgtttc
     2161 aattatttat acttttttat caaatttttt gctaaaaaag tggaagcttc ttccattagt
     2221 ggtatttgta ccatatgtcg ctgtgttttt gaggaacgaa aaacaatttc ttttccgtaa
     2281 gacttatgcc gatttttttc tacaaatcgt tccgtttgta tataagggat tttttcgtca
     2341 gcttgtccat gccaaaagaa cagaggacgt ccagctaact tttccggctg caatgacaaa
     2401 tcatagctct taacccatga aatcaaatca aaatagtctt ttggtacgac ataattatat
     2461 tccgatgcgc gccttacgat ttcttttcca tattccaaag gtgcagggga acccatcaaa
     2521 cacacgcccg ctactatatg aggatgttta gccaacaagg cgcatgttgt cattcccccc
     2581 atcgaaaccc cgccaactgc aagctgatca tgtagaagac ctcttttttc taaatgcttg
     2641 ataagtgtgt caaattcaaa cagatttccg tagatactat cccaaaatgt ataggatgga
     2701 atagaagaaa ccggctgttt gcgctcccca tgattcatcg catctggcag gatcacacga
     2761 atgccctgct gcgcaatttt tcttccttgc gtcaaaacta actccttact tgtctgccat
     2821 ccgtgataat agatgacaac gggaatacgt tcctctctta attctttcag aacaacttct
     2881 aacactggaa tatgatggac agaacgtttc aatataattg gattcaattg ttactcacct
     2941 attttcatta aatttcctga tcgccatagc aaccatgctc caacagttgt ctggaacagg
     3001 ttcagtgtac caaataatat aaatgcagga ctgaatggct cgggaaataa gcacagagaa
     3061 caaagtccca ccactcctaa tgctagcgaa aggagtggat aaatagaaaa attcaaaaaa
     3121 ctggcaacgc taccgaaaat attaccaata aacagtatca aaaagaaaaa aacgcttaaa
     3181 attaaaccat ctgtcagcga catctgaaaa acaatgttct ctacctcaat tggctgttga
     3241 atcgaataga cacctaagta tatactgaga aggagactta gcacgtggct ggcacaaaac
     3301 acacgattga ccgttttcat tttaatcacc taacctatct ttataattct attataatca
     3361 ttttatcatt tttttgtatg tataaagttt caatttttct tcacttacga ttttttttga
     3421 tattgttgtt atataaatga aaggagtttt acaaatgatt gatccaaaaa tgttggggat
     3481 gatcttcctc attaattttg cttatatcac tctgaacacg ttacgtttca tgttgacaat
     3541 gaaaggctat cgactgattg ctccacttgt cagtatggtc gagatcacga tttatatcgt
     3601 cggcttatcc cttgttttag atcgtttaga taatccactg aatttatttg tctatgcgtt
     3661 aggttacgca gtaggtatca gtgtcggaat tttgattgaa gacaaattgg cattaggata
     3721 catcatggtc acaacgattt tgccagcaaa tagcgcagaa gataaaagtt tgccgggtat
     3781 actgagagaa cacggttatg gtgtgacaca gacttctgca atggggctgg aaggtgagcg
     3841 gctagttctc gaaatacttt ctcctagaaa aagcgaacgt gaactgtatc gcctgatcaa
     3901 agaaatcgaa gagcgtgctt ttatcatttc ttatgagcca aaatatattt caggtggttt
     3961 ctggactaaa aaagtgagaa aacgacaagg aaaataactc acaaacttaa aaactgtcta
     4021 atatctaaat taaattttaa atgttagaca gtttttattt attattttga ttaaaaaaca
     4081 tcgacatata ctcatcaatt tgttattata ctttatatac ttatcaattt aatttggaga
     4141 tattatggaa attcaacgta gaaccactaa ttttttacat ttaggctctt tttttgcaac
     4201 tttgacgatc ttattttttc tatgttattc ataccttaag acctcacatg cgccttctag
     4261 tgcattaggc tggttgcttt tggatcattc tgatatcaca gaagccgaag aaaatccgtt
     4321 ttttttgatt ttgtcttctc tttgcggctt actgttttct gtttatttcg gtgcctattt
     4381 ttttcatcga catcctggta cgttaaacga agcttcaagc gtattgattg catttaaaac
     4441 gttttctttt ttagcaattc ttttttattc gtttaatcac aatacgcttt tcttctttat
     4501 catgaatggt ttagtcgtga ttttatccat ttttacttat tttatttttt cactcatttt
     4561 tagccaattg aaatgtccgc tcttctttcg attagttcct gtcacctgtt ttatttacag
     4621 cttgttttcc ttttttgtcc ttgctttaat gccactgacc aacccttcga cgatccaagt
     4681 cgcaggaaat ctgttggata tgctcttcat cgtcagtcta ggtgtattta tgtggcaaag
     4741 aaaaaacaaa caaaataaaa agcattttga agcctgacta ttacgacttc ataaatgctt
     4801 ttttagtttt aaacacttca atctaaaaga ataacccaag attggataaa ttattaatga
     4861 ctcgccattc gtttcttagt gattctggat tatcaaatgg acttcctaat ctaatcagct
     4921 agttcttccc ataaaatgcc atacttgact gaatcataat attgattatt taaaaatcta
     4981 acttttctta tcactacttc ctttgtcatt ccacatttct caccattttt ttgcattcct
     5041 ttgtttccag accatgtagt gaatctaata ggtggtagat aggcaaaata ctcaaataat
     5101 tcttttaacc ataaaggaag agaaaggctc ctcctgcaag agtcagtata atttttgctt
     5161 actgctctct taggttttac tcactaaaac tgttccatat tctttgatat aagaaaatgc
     5221 atgcggtata aatttcttgc tcagctcaga aaagatttgt cgtttaagaa aggccatttc
     5281 ctccattgtc aatgaaggat ccaatgttgg taattcaatc tcaaaattgt ttttttcgac
     5341 aataggtaat tctaatgtga atgattcttc ttcctttata gtattcgtca caagtagaga
     5401 ctgatggaaa atactaggcc ggcgattttt atatttattc ttacgtatcc tgtcaacgat
     5461 aacttctatt tgatcaaaaa agtacgattc atttacgtct ccaataagtt cggctaattc
     5521 tttgaatgcc ttcgttacag gaagctgatt cttctgtaca aaaaagtaat tagccaaagt
     5581 aagcgggaag tgttctatag aggaaagtgt gataaaacgg tacttctcat ttcctgcagt
     5641 tgtcctcagc aattctgtct ctcggctgtt tcttactcca taaaaatacg cttctttccg
     5701 ttcatcttcg aaacaatacg cccaaataac ttctcccatc gctaattttt gttcatcaac
     5761 agagagtctc ttcaattgtt cagctatggt ttcagcagcc gaagtggctt gttgcaagta
     5821 atctttagga tcaaatattg gaaagttcaa atgatatctt cgttcttccc tcttgattaa
     5881 tcctgcatcg atcaataaat caagaaaatg ggaaaattta ttttgcgaaa attctttttt
     5941 caattctctt agaatcgttt cttgttctcg atgttcatat agataatcgg tcaatgaaga
     6001 aaaaagcgga tcatcggtca gtttttgata aaagttttcc ttattttttg aataatagtt
     6061 catatatgtt ctcttttcta tccgttttat tttacttaaa aggcttttac gtaaaaagga
     6121 ggaagaacaa gagtcataat tgactgttat tctcctccat ttcttcggcc tttagtatgt
     6181 tctatgtttc tttttatatc aatcgcttat ccattcattt tgaatgcttc atactaccat
     6241 ttgattagct aaatacagcc gtcaattgag gtacgacttg ttttttacgt gaaacgacac
     6301 ctttaagcaa agcacggttg ttggtcaaag ttgtttggaa agcttcttct actttttctt
     6361 ttcctttacc agcaacaagt aattctgaat cactgtctaa gatgtttgtg acaactaata
     6421 cgaacagatc gtagccattt gctttcattt catcttccat tgctgcttct agctctgctt
     6481 gtcgatccat cacttcatta aggtccactg tattgacttg agcgatacga acgttggaac
     6541 tgcccattgg gaaacttttt gcatccaaat caagaagaac cgcagctgat ttatcactta
     6601 gatttgttcc tgctttcaac atttcaagtc cataagtgtt tagatcgact tcagcgattg
     6661 ctgcaagttc ttttgcagct gcttcatctt caggcgtaca tgttggtgat ttgaacaaca
     6721 atgtatctga aatgatagct gagagcatga ttcctgcgat ttctttagga attgttactt
     6781 ggttttcttt atatagtttt aaaataatcg tacttgtaca tccaactggt tctgcacgat
     6841 agtacaatgg attagctgtt tcaaagtttg caatacgatg gtggtctact acagctaaaa
     6901 tatttacttc gctgatatct gaaatacttt gttggaattc attatgatcc acaagcatga
     6961 catctgcatt ttctgcttta gctgactcta ctactcgtgg tgcagatagc tgaaagtggt
     7021 ctaatgcgta ttgtgtttct tcattaggtg tacctagagc aactgcttct gtttctttac
     7081 ctaattcatt ttgcaagtgt gaaaacgcaa tggctgcccc gattgcatct gtatctggat
     7141 tttggtgacc aaaaactaaa atttttgaca tgtcattcgc tcctttgtga tttctcctat
     7201 ttaataatag caaaaaaacg gaaagaagca atacttatct tctttccgat gcgacctatc
     7261 gcgttaaata ttttgtatac tcttctgtat gaagcaatcg tttagcattt tcgacacgtt
     7321 cttttgtagg tggttcgatc ccttctaatg gatatttcaa accaagtgtt tcccacttgt
     7381 attttcccat cgtatgataa ggaagaatct ctactctgtc tacatttgac aaggtttgaa
     7441 taaagttgtc tagtcggatc aaatattcat cataatcact tcgctcagga actagtacat
     7501 ggcgaatcca aaccggtttg ttgatttttg acaaatattg agccatttct aaaatatttt
     7561 cattgtccca atgcgttagt tttttatgct cttcattatc gatgtgtttg atgtcaaata
     7621 acagtaaatc tgtatacttc atcaattctt gaaaacgtga aaagaaaggt tcttcatacg
     7681 taaaaggttt tccgcatgta tccaatgtag tatgcatctt ccgctctttt gcttttttaa
     7741 atagttcgat caaaaaatcg atctgcaata gaggttctcc accactaaca gtaataccac
     7801 ctttatctcc ccagtaaggt ctgaagcgtt ctgctttatc taaaagttca tctgctgtgt
     7861 attctttccc tccgccaata ttccaagtat ctggattgtg acaaaattgg caacgcatgc
     7921 ggcatccttg cataaacacg acaaagcgca gtccaggtcc gtctactgaa ccgaaagttt
     7981 cgatagaatg aacatagccg attgtttttt cttccattta agttcacatc ttttctttga
     8041 tttcatttta agtacaagtt agctgtcgaa gcgaagcttt ctgaacaaaa tttttcgcaa
     8101 ttgccgcttc gttatgtgta aaatttacag ggacggaaca tacaagtcct gcccctgtaa
     8161 atcgtttctt tcaaataagc atactgatta ttcgattttg atgcgatggc tattcaacgt
     8221 cctttcgacg ctaagtcgaa aaactttttt cacgaacttt ttacattctg tcgtgtgaag
     8281 ttctcatcaa gacatctaat tgttgttctc ttgtcaagtc acggaattta actgcataac
     8341 cagatacccg gatcgtcaag ttcggatatt tcattggatt ttccattgca tcacgcaata
     8401 aatcattact gaatacattg acattcaaat gataagctcc ttttgagaag tatccatcta
     8461 agacattacg caagttttcg attcttgtat cttgttcttt tccaagacct gaaggattga
     8521 ttgtttgggt atttgaaatt ccatccaatg cacagccgta attcatttta gctgttgagt
     8581 tcaggctagc taataaccca ctttgttccg cattgtaact tggatttgct cctggcgcaa
     8641 gtggttttcc agcttcacgg ccatcaggag tatttcctgt atttttaccg taaacaacat
     8701 ttgaagtaat cgtcaataat gaagttgttg gttttgagtt gcggtaagtt ttatgacgtt
     8761 tgatttgtcc catgaagtat tccaagatcc atgaaactaa ttcatctgca cggtcatcat
     8821 catttccgta tttagggaag tcgccatcca cttcaaaatc aactgctatt cctttttcat
     8881 tacggattgc ttttactttt gcatatttga tcgctgacaa gctgtctgcc gcatgagaga
     8941 tcccagcaat acctgtagca aatgtgcgtt gcaagtcaga atccatcaaa gccagctgag
     9001 ctgcttcata agaatattta tcatgcatat aatgaatgat gttcaacgta ttgacatata
     9061 gttctgctaa ccaatccatc atattcatga agttatgttt cacttcttca aagtctagta
     9121 tttctgatgt gattggacga tattctggtc caacttgggc gccagtcact tcatcgactc
     9181 cgccattgat ggcatataat agtgttttag ccaagttagc acgagcgcca aagaattgca
     9241 tgtcttttcc gacaactgtt cctgatacac agcaagcaat cgcacaatca tcagaacccc
     9301 attgttcacg aagctgatta tcattttcga attgaatcga agaactttcg atagcgattc
     9361 tgctagcata tgttctgaat ccttctggaa ggtgttcaga atacaaaaca gtcaagtttg
     9421 gttctggtga aggtcccata tttgttaatg tatgaagcag acggaaatca ttctttgtaa
     9481 ctaatgaacg accatctaat cccatacctg caatggataa agtagcccag atagggaatc
     9541 ctgagaataa ttggttatat tcaggtgtac gcgcgaattt gaccatacgt aatttcatta
     9601 ctaagtggtc gatcaattct tgtgcttctt tttctgtgat tactccatta tccaaatcac
     9661 gttggatata gatatcaagg aatgctgaga tacgtccgat tgacatcgca gcaccatttt
     9721 gtgatttgat tgctgctaaa tatccgaagt atagccattg gacagcttct tttgcatttg
     9781 cagctggttt agaaatatca tagccatatt tcgcagccat ttctttcatt tgcgctaatg
     9841 cacgatattg ttcagtgatt tcttcacgta aacgaatcac ttcttcactc attgtatgat
     9901 aaccagtcat atcatgatct tttttctttt gttccatcaa atagtcgatt ccatacaatg
     9961 ccacacgacg atagtcaccg atgatacgtc cgcgaccgta agcgtctgga agccctgtaa
    10021 tgattttgtt tttacgagca gcacgcattt cagcagtata agcgtcaaat acaccttgat
    10081 tatgtgtttt gcgccagtca gtgaaaatat gtgtcaattc atcgtttggt gtatagccat
    10141 tgctttttag cgcattgtta gccatattga ttccgccgaa cggcatgaat gcttgtttca
    10201 aaggtttgtc tgtctgtaga ccgacgattt tttccaaatc tttatccaaa tagcctgcat
    10261 cgtgtgaagt aatagttgat acgatttctg tgtccatatc atagacaccg ttacgctcga
    10321 attggatatc atttaacttt tgcaattcat cccataattt ttctgttgct tctgttgggc
    10381 cttccaaaaa gctgtcatca ccgcgatatt cagtgtagtt attttgaata aagtctctag
    10441 tgtctacgcc ttctttccat ttggtacctt taaatccatt ccattgctcc attgtatgga
    10501 cctcctaaat ttctgtttta tgtaacagtt tttacgtgat tagtataaca cagcacccta
    10561 tctttgcaag ggttttcttg ttttttattt aacaattttt atgttttatt tacatatatt
    10621 taaatcaacg ttgacatacc aacatttaag cattgtgaac actttcatct ttttttgcaa
    10681 acgtataagg atttattaaa aatgaatgaa aaagagacgc cacctgtatt aatacagatg
    10741 gcgtctcttc attaaaatac tacaacagtt acgactcgat gaaaatctca ggagcatcga
    10801 taacttcgat gacttctcca ccttttttct catccatcgc aaatgatcca ttcgatgttc
    10861 gatcacctat agggaaatca gttgggtcaa tcgttaactg ctgccctttt tgattcatca
    10921 aggttatttc ttttgatctt ccttccgaca tatagataat acggtgcgga ttttttttca
    10981 gctcacgcat gaccgtcact ccccgcttcg cacgtgaagt ttggctgatt tcttgagcaa
    11041 gcatgcgttt aacagctcct cgctgagtaa cgattacgat cggcgtatca ccttctgcta
    11101 acacaagcag cccgttgata atcctatctc catctttcaa attcatagac ttcactccag
    11161 ctgcctttgc tccaacaacg ggaacatctg ttaaaggata gcgcaaaccc attccttgat
    11221 gcgttactaa gaaaacatct aagcctggct gatcttcttt cagatacaca gcaatcagtt
    11281 catcttcttc cgcctttagc ttcatacagc tgagcggacg gctcttatag gtgcgccatg
    11341 gttcgaactc tgtcattcgt gtttgcttga tcaatccatt cttgctgatg aatacaaatg
    11401 ttttctctgc ttgaattttt tgatatggga aaacagctaa aatcgattcg tctacagaca
    11461 gattggagat tgtttgagaa atgtgctcac cagcatcttt ccatttcaaa tcgggtagtt
    11521 catggacagg gcggtagatc acatttgctt tatttgtgat cagaagaaca tggtccaatg
    11581 tactcaattc acctgagtat agcaagtagt cgccttctcg catcccgatt tcttctggtt
    11641 tggaagcact ataagaacga aggcttgttc gcttcacata gccttctcgc gtcacactga
    11701 caattacgtc ttcttgtgcc actaagactt gcgtatcaat tttgatttct tcgatttcgt
    11761 cttcgattgt cgttagacga gcggaagaat aatttttttt cacttctcgc aattcttttt
    11821 tcataacaga gaaaagctct ttgtcatttg aaaggatttt attcaactct gtgatttgcg
    11881 caatcaagct ttcggattcc ttttgaagct ctgtgatatc cgtgttcgtc aaacggtata
    11941 attgcaatgt gacaattgct tctgcttgtt cttctgtaaa ttgaaagacg tcgactaaat
    12001 tctttttggc gtcttttttg tctttacttt cacgaatcgt tgcgattact tcatccagaa
    12061 tcgataaagc tttcatcaat ccttctacga tatgctgtct tttttgagct ttggctaaat
    12121 cgaattgact acgttttgtt atgacttgct ttcgatgctc gatataactt cttaaaatgt
    12181 ctttcagtcc aacttgatgc ggagtcatat ggtcgatagc gaccatattg aaattgtaat
    12241 tgatctgtag ctcagtgttt ttgaacaaat aattcaaaat tccttgagca ttggcgtctt
    12301 ttttcaattc gacgacaatt tgcagacctg ttctatcact ttcatctcgt acttcagcta
    12361 ttccatcgat tttcttattc aaacggatct catccatttt tttcaccaag gtcgctttat
    12421 ttacttcata tgggatttct gtgatcacga tttgctgttt attacccttc attgcctcta
    12481 tctttgtttt cgaacgaaga ataacttttc ctcgtcctgt ttcataagct tttttgattt
    12541 catcttttcc ttgcaagatc cctcctgttg gaaagtcagg tccaggaata tattccatca
    12601 atttttcaag gctcgcttgt ggatgatcga tcatatagat tgtgccatcg atcacttcag
    12661 ctaagttatg agtcgggatt tctgttgcat atccagcaga tatacctgtc gatccattga
    12721 cgagaaggtt tgggtatcga gctggaagga ctgttggttc tttttctgta tcatcaaaat
    12781 tccacacgag atcgactgtt tctttctcga tatctgctag catctctcca cttaacttgg
    12841 ataatctggc ttcagtataa cgcatcgcag caggtggatc accgtccata ctcccgttat
    12901 tcccgtgcat ttcgatcagt acttcccgca acttccagtc ttggctcatg cggaccatcg
    12961 cttcataaat actgatgtca ccatgaggat gataattccc catgatattt ccgacagatt
    13021 ttgctgattt acggaatcct ttgtcatacg tattgccatc tttgttcatg gcaaataata
    13081 tccggcgttg cactggcttc aatccatcac ggatatcagg cagtgcacgt tcttggataa
    13141 tatatttgga ataacgtccg aagcggtcac ccatcacttc ttctaaagtt aattcttgta
    13201 cttcttgtcg attttccatc aggtgacctc ctattctact tcaaataact gattgttttc
    13261 atttttatct gctcgttctt catctaataa atcattagaa actgaaggag agatttcagt
    13321 atcctctttt ttatctaaga tactgccatc ttcttcgagc gtaaattgca cgtgattttc
    13381 gatccatttt cttcgaggtt ctactttgtc acccatcagt gtggttactc gacgttcagc
    13441 ttgcgcagca tcatcgatac ggacgcggat caacgtacgg gaagtcggat ccatcgttgt
    13501 ttcccacaat tgttccgcat tcatctctcc aagtccttta tatcgctgca gcatatatcc
    13561 tttgccgact tttttaatca ttgctgccaa ttcgtcatct gtccacgcat attcaatgac
    13621 ttgcttcttt ccttgtcctt tggatacttt atataaaggc ggtaacgcga tatatacttt
    13681 cccagcttca atcagcggtt tcatataacg ataaaagaaa gtaagcagta atacttgaat
    13741 atgggcgccg tctgtatccg catcggtcat gatgattact ttgtcgtaat tacagtcttc
    13801 gatagaaaac tcaggaccga ctcctgcacc aatcgtatag atcatcgtat tgatttcttc
    13861 atttttcaag atatcttgca ttttagcttt ttcggtattg atgacttttc ctcgtaaagg
    13921 tagaattgct tggaactttc tgtctcttcc ttgtttagca gatccgccag cagaatcccc
    13981 ttcgactaaa tacaattcat ttttcttagg gtttcgggat tgtgcgggtg tcaattttcc
    14041 tgaaagaaga gattcgcctt ttttacgctt cttcccattt cggctttctt ctctggcttt
    14101 acgtgctgct tctctcgctt ctctggcttt gattgcttta cggaccaaca tctggctcat
    14161 ttcgctattt tcctgcaaat aaaagcccat ttgttcactg atgacgttat ctactacggt
    14221 acgagcaact ggcgtaccaa gtttttcttt tgtttgccct tcaaattgca gaagattttc
    14281 aggtacacgg atagaaagta cagcagctaa cccttctctg aaatcactac cttccagatt
    14341 cttgtcccgt tctttcagca aaccaacttt tctcgcatat tcattataag atttcgtcat
    14401 cgccgctttc ataccagctt catgggttcc cccatctttt gtccgaacgt tattgacaaa
    14461 agataaaaca ttttctgaat aaccgtcatt gtattgataa gcgacctcta cttcgatacc
    14521 ttctttttct cctgaaaaat aaacaactgg tgtcaatgta tctttttctt cattcagata
    14581 atcaacaaat tctttgattc cttcttcgta atgaaaaatt tccttgactg gttcttctcc
    14641 acgaagatca gaaagagaga ttttaacgcc ttttagaaga aaggcagatt ctcttaaccg
    14701 ttcggctaaa atttcatatg aaaatttcgt tgtactgaat attgtatcat ctggtaaaaa
    14761 atggacagtc gtcccgtttg ctttttttgt ttttcctatc tttttcaaag taccgtcagg
    14821 tttaccgccg tttttaaatt tctgctgata ctctacgcca tctctaacga tcgttacaat
    14881 tagccatttc gataaggcat ttaccacact ggctcctaca ccgtgcagtc caccagaagt
    14941 tttgtaccct ccttgaccaa actttccgcc agcatgcagc acagtaaaaa tcacttcaac
    15001 tgttggtatt ccggaagcat gcattcctac aggcatccca cgtccgctat ctgcaacagt
    15061 tatactattg tcttcatgga tggtcacatc aatctcactg ccataacctg ataatgcttc
    15121 gtcaactgca ttatcgacga tttcatagac taaatgatgc aagcctcggc tgtcagtgga
    15181 tccaatgtac atccctggtc tttttctaac tgcctctaat ccttccaata cttggatgga
    15241 cgagtcattg tattcgttgt ttactttttt tgccaaggca aacgctcctt caataatact
    15301 ttgcggcaaa accgcaccac gattatcata ctagaaaagg tcgtgaaaaa aaagagggta
    15361 gacgagaaat caattttaat tatctcctaa ccgctttttc gacattcccc tctatcgatc
    15421 atctcttctt tgtggtcatt tttatttttg gttttctttc aacttaccaa gttcaatctt
    15481 aatacaacga tccattacga tatctgtacg tcctgctttt cttagcatct cagctgcttc
    15541 tttgttttca atccctaatt gtgcccagaa cactttagca tcagttttca aaaaatcacc
    15601 tgctatttca ggtaaaaatt cactccgtct aaatacatct acgatatcga ttggatcatc
    15661 gatttctgtc aaattcgcat agactttctc gcctagaatt tcttcaccag ccaataatgg
    15721 atttactggt atgattttat atccattgtc ttgcaatacc tgtgcaacca tgtaacttgt
    15781 tcgttctggt ttattgctca agcctacgac ggcaatattt tttgcttctt ccaaatagtt
    15841 gaaaatcgtt tcttgtgttg gattttttac agacatttct gccacccctt gcttttcttt
    15901 ttattccatc tattatactt gtatctttca ttttttcaat tgttcctgtt acctattcat
    15961 gctaaaataa aggatagttt tcaacttgaa agagggtaaa aaatgaaatt gatcttatta
    16021 cttattttag catatctttt aggttctatt ccttccggtg tctggattgg caaattgttt
    16081 ttcaaaaaag atatccgaca acacgggagc ggtaatactg gaacgaccaa tacatttcgt
    16141 gtattaggca aaacagccgg gattgttgtc ctgcttatgg atattttaaa aggtacattg
    16201 gctacttgtt tgccgatgat cttccacctt tctgggatta accctctttg gtttggggta
    16261 tgcgccattc ttggccatac atttccgatt tttgctgggt tcaaaggagg aaaagctgtt
    16321 gcgacaagtg caggaatgct tttaggattt aatcctgtat tttttgttta ttcttgtatc
    16381 atctttatcg ttagtttatt ttgtacaagt atggtgagtt tgagcagtat gatcagtgca
    16441 gtattgatca ctttatctac gatcgtcctt ccttatgtag ctcctgtgat tttggctaag
    16501 cccaattggt tactgacgat cattgccttt ttagtttctt cctttatctt ttatcggcat
    16561 aaagacaata tcaagcgtat cagaaaccat actgaaagtc gaatcccgtt tggtttgaat
    16621 agcagcaaga aaaaatcctg atactttttc attccaaaga gataaaacgg gagtgtgaca
    16681 aatcgatgtc acactccctt attacttaca ggatagcttt tttacacacc ttggtaaacg
    16741 gtgttccctg ctaacttctc ggtcacaacc tcttacttca ctgtgatgga atattttgtc
    16801 ttgaagattt catgggcacc tagttgattg atccctactt tttccatcaa gtttccacta
    16861 ctgttcaccg tgtcagcgat tccgcaccac ggttcgatac aaacgaaagg cgcttctttt
    16921 ggataaggag accagatccc tacataaggc atatctttat agcttaaagt tacgctatgg
    16981 gaagattttt cacttcgaat ggcgtacgca tttagacccc ttgtttcaaa aaccaaggca
    17041 tcttgctcaa aaagttcacg attcaaagcc agattcgtat ttgtctgacc taacgtcttt
    17101 tgatccatat cgatgaatgg gcctttcaat ggtaggcgta atcttgattt caatggagaa
    17161 aatgccagat agaaatcttc aaatgacaat ccttcttcaa gaggtacatt gaaagcagga
    17221 tgtccaccga tagaaaagaa catttctttt tcaccggtat ttgccacttg ataatgaacc
    17281 gtgatgccgt ctcctcctaa ttcatagctg atcactaatt caaaatcaaa tggatacttt
    17341 tctaatgtct ccggcgtact ccttaagatg aaactagcca tttcttgtcc atgttcaatg
    17401 acttcaaaat ccatatcccg agcaaatccg tgttgcccca tttgatatgt tttcccttga
    17461 taaacatact gatcatcttt taaacgtcca acaaaaggga ataaaaccgg tgcatgtctg
    17521 ccccaatacg ctggatcacc ttgccagata tactctaaac catgttcttt tgatttcaga
    17581 ctgatcaatt ccgcaccgtg ttcagcgatt tcggcgatca gctggtcatt ttgaataata
    17641 acagtcattg ccattcctcc aacttcgact tactttttaa aatgggaagg ctggaacaaa
    17701 catttctatc tgccccagtc cccttacttc taataactgt gttttgctaa ctcgtcttta
    17761 aaacgggaat tcaatacttt taaataagtt cccttcatgc ctaacgaacg agattcaatg
    17821 attccagctg attccaattt tcgtaaagca ttgacgatca cagaacgagt gatgccgata
    17881 ctatcggcaa tattagaagc agtcagacga ccttcatctc catccaatgc ttcaaagata
    17941 gcttgtacag ccttcaattc gctataagat aatgtgttga ttgccatttg gacagccgtt
    18001 gcactccgta catctgcttc gatgcttcga gacttctgat aaaggatctg cataccgaca
    18061 accgtagctc catattctgc taagactaga tcgtcatctg caaacgattg ttccatacga
    18121 gataagataa tcgtacccaa acgttcgcca gctccaaaga tcggaacgac agtcgtcaga
    18181 ccaaatggat atttctctct tgactctacc ggaaatgcag taatatcact agtaattggg
    18241 atattagctt cagttttcat caactgtgcc actgtatcag tataagattc aggaaattgt
    18301 ttctcaacaa acattgtttt gattcgttca ttgttcacat cgtgacgttc attataacct
    18361 agtaatactc cgtcacttcc tatgatatag gtgttgctgt caagaatacc acctagtgtg
    18421 acagccattt tatcgtaagg caaatctgct tgcatgtcaa aagtattctt ttgctgcaaa
    18481 agtgtgttta tcttgcgggt tttcgttagc aagtctgtca ttgtcgtcct ccgatttttt
    18541 ataaaatata gcgacttaaa tcttcatttg ccgcaattga gccaagtttg tcatccacat
    18601 atgcttctgt gatcgttatt tctcccattt gcatatctgg tgcttcaaat aatagatctt
    18661 ctagtagttt ttccaaaatg gtatgcagtc tgcgtgcgcc gatattatct gtgtcacggt
    18721 tgacatcgaa agcgatttga gcgatccgtt caattgcttc ttttgtaaac gtaactgaca
    18781 cattttctgt accgatcaac gcgacgtatt gttttactaa agcattattt ggttcggtca
    18841 gaatcttcac aaaatcttca gctgtcaaat catctaattc tacccgtatc gggaagcgtc
    18901 cttgaagttc tggaattaga tcacttggtt tagacaaatg gaaagctcct gaagcaatga
    18961 ataggacgtg atctgtttgg atgatcccgt attttgtgtt tacttgtgaa ccttcgacaa
    19021 ttggcaatat gtctctttgg acgccttctc tggatacttc tccagattgc tgtgatttag
    19081 aagtgatctt atcgatttca tcgataaaaa taattccact ttcttccgct aatttgattg
    19141 cctcactatg gatatcagca tccttgacta attttgctga ttcttccttg atcaataact
    19201 ctaaagcttc tttgactgtt accgtccgtt ccactttttt cttcggtgtc aaagcaccta
    19261 gagtttcatt taggtcgatc cccatttgct ccaatccatt gttcatcatt ggtgcaggtt
    19321 tcttttggtc ttcaacttca atggttactt cacgactatc cagtaaaccc ttttgtagtt
    19381 gttccaagac tgtacggcga ttgattcgaa tatcttctgt cacttcttct tgcggttcag
    19441 cggtattttg ctgactagca ttaaaaagat tcatcatttg ttcaaattga ttagtggatt
    19501 gtttctgttc ttttttgata ccaggtacca agatttttac caaacgtttt tctgcatttt
    19561 ttttagcact tgtgtataca cggctgtact gctctttttc tacgatttgg atcgcatttt
    19621 ctactaaatc ccgaaccatt gattctacat ctcttccgac ataaccgact tcagtaaatt
    19681 ttgttgcttc gactttgaca aatggtgcat tgacgatttt tgccaaacgt cgtgcgattt
    19741 ctgttttacc gacccctgta ggtccgatca tcagcatatt ttttggtgta atatcttgtt
    19801 gcattttttc atctaactgc aaacggcgat agcggttacg caaagcaacg gcaacagatt
    19861 tttttgctgc gtgctgtcct acgatatatt catctaattc acttacgatt tgtcttggtg
    19921 tttgttttaa ttcatacata tctactcacc tcttacagtt cttctacgat aatattatga
    19981 ttagtaaaga tacagatatc tgcggcaata tttaacgcat tttctgcaat ttctttcgca
    20041 ctcatgtcgt tttgagcata gtttttcatt gctcgggctg cagccaacgc aaaattccct
    20101 cctgatccga tagctaagat accatcatct ggtgtgataa cttctcctgt acctgaaaca
    20161 agaagcattt ctttatcatt cattacgatc agcatcgctt caagtttttg catagattgc
    20221 tgtgtccgcc attcttgagc taattcgaca gctgcgcgtt gtagattccc gttatattcg
    20281 tttagtttgc cttcaaattt ttcttctaaa gtaaacgcat ctgcaacact tccggcgaaa
    20341 cctacaacaa cttctccatt gtagatacgt cgtactttct ttgcagtgcc tttcatgaca
    20401 acagattcac ccattgttac ttggccatct ccagccatag caaatttccc gtctttttcg
    20461 acggcacaga tagtggttga atgaaatggt gattcgacca tgtttgtttc ctcctaaatt
    20521 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
    20581 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
    20641 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
    20701 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
    20761 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
    20821 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
    20881 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
    20941 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
    21001 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
    21061 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
    21121 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
    21181 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
    21241 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
    21301 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
    21361 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
    21421 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
    21481 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
    21541 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
    21601 agagcgagac tatccgtctc accctcttca tggagtttcc ttactcattt gggattgcag
    21661 ttttttctaa ttctgttttt attgcctcat tagcttttaa agctcgttgt gcaattgctt
    21721 catatcttgc ttttttgtct cgaatgcgct ctggcagttc aggaaacaga ccatagttgg
    21781 cattcatcgg ttggaaatgt tttccttccg catgagtaat gtagtaagcc atacttccaa
    21841 tagctgtttc ttgtggaaac tcgactggtt ccatttcttt tgctaagcgt gcggcgttga
    21901 ttccagcaag tagtccgctc gctgcacttt caacatagcc ttctacacca gtcatctgac
    21961 ctgcaaagaa cagatcttcg cgtttcgttg attggtaagt cggtttcagc aattctggcg
    22021 aattcataaa gctgtttcgg tgcatcacac catagcgaac gaattcagca ttttctaacc
    22081 caggaatcat ccggaaaaca cgtttttgct cgccccattt caagtgagtc tgaaagccca
    22141 cgatgttgta taaagaagct gcagcattgt cttgacgcag ctggataaca gcataaggac
    22201 gtttgccagt tttaggatct tccaatccaa ctggtttcat aggtccgaaa agcatcgttt
    22261 tgaatcctcg ctgtgccatt acttcaattg gcatacatcc ttcaaaatat ttttctttct
    22321 caaaagattt aagttctgcc acttcggcat tgattagtgc ctcatggaaa gctttgaatt
    22381 cctcttctgt catcggacag ttcaagtaag ctgcttctcc tttgtcatag cgggacttca
    22441 aatatacttt atccatatcg atcgtagctt tgtctacgat tggtgcagct gcatcataga
    22501 aataaaaacc atctgaacca ttgaatgctt tgatgctttc tgctaatgga tgagaagtca
    22561 aaggtcctgt tgctactacg gtgattcctt ccggtatttc agtaatttct tcgtttatca
    22621 ctgttactaa tggatgctct ttgacttttc gggtaattgt ttcagaaaaa gaatcgcggt
    22681 caacagctaa ggcaccacca gctggcactg ctgtttcatc tgccgaagtg attactaccg
    22741 aattcaatcg gcgcatttct tctttcaata accctactgc attagccaaa ctgtttcctc
    22801 ttaatgaatt ggaacatact agttcagcaa aattacttgt gtgatgcgcc tcagtcgatt
    22861 tgactggacg catttcatat aaacggacag ggactcctgc ttgtgcgatc tgccaagcag
    22921 cttcacttcc ggcaagccct gctccaacaa catttaccat tttcaatgaa agaacctctt
    22981 ttctattatt tttggatgtc ttcttcgtaa tctccattta cacagactac ttgtttaccg
    23041 cctttgactt tcttctcgac cagatatccg tcacattttg gacaatttct gcccactggt
    23101 ttatcccaag aagtgaattc acattctggg aaacgggaac aaccatagaa tagtctgttt
    23161 ttcttagatt ttcgttcaat gacttgtcct tcatgacata ctgggcaagt cacaccgatt
    23221 tcttttacga ttggtttcgt attccgacaa tcaggaaaat tactgcaagc ataaaacttc
    23281 ccatatttcc ctaattttat caccatcgga tgtccacaga catcacaatc gaatcctgct
    23341 ggttcatctt tgatttgaat tttttcgatt ttttcttccg cattcgtcag ttctttttca
    23401 aacggtttat agaaacgatc aaccactttg acccacgctt ccgtgccttc ttcaatttta
    23461 tccaaatctc cttccatttc tgctgtgaaa tggatatcga cgatttgagg gaagaattca
    23521 cagattaatg aattgacaat ttctcctagt tcagtcggct caaatctttt ttgtgtcaat
    23581 tttacatagt agcgtctttg gatcgtatca agtgttggtg cataagtgga aggtcgccct
    23641 accccgtttt cttctagtgc acgaatcaac gtagcctcac taaaacgtgc tggtggttga
    23701 gtaaaatgtt gttttggttc aatatcaaca gattggacta catcgccttc ttcaagttct
    23761 ggaagaatat tttctttctc ttctttgccg tcatctctac cttccacata cacttgcatg
    23821 aatcctttga atttgatttt tgaaccgttc gcaatgaaga tgacaccatt ttgttctaat
    23881 gtcactttca ttgtatctaa aatagctggt gtcatctgac tagccacaaa acgtgaccag
    23941 atcaatgtat aaagtttcaa ttgatcttta tctaaatatt gtttgagttc atctggtgta
    24001 cgaagcacac tcgttggacg cacagcttca tgggcatctt gtgcgccttg agcatttttg
    24061 atctttcggt gatcatgtct agaaaattct tttccatacg tatcttcaat aaatgctgcg
    24121 gcctctgctt ttgctgtatc ggcaatccgg gtagaatcag tacgcatgta tgtaatcaag
    24181 ccgactgttc cttgtttacc caaagtgatt ccttcatata attgctgtgc taccatcatg
    24241 gtttttcttg ttcgaaagtt taacttacgg gcagcttctt gttgcaggct acttgtagtg
    24301 aaaggattcg cagggtttct tttgcgctct ttcttttcta cttttttaac ttcgaagtct
    24361 gttccatcaa ttctagcagt gacttctttt acactttgtg catctggtaa tttctttttc
    24421 ttaccgtcga ttccccagaa atttgctttg aatttcttgc gagctttttt aaaattaccg
    24481 tcgatgctcc aatattcttc tggaacaaat tctcggatct ctttttcacg gtcaataata
    24541 atttttaaag cgacagattg gacacgtcct gcacttaacc ctttcttgac cttacgccag
    24601 agaataggac tgatcgaata accaacgatt ctgtctaagg tacgacgagc ttgctgtgca
    24661 tctactaaat ctacatcgat cgttcttggt tccttaaaag ctgctttgac tgcgtctttt
    24721 gtgatttcat tgaagaccac ccgatttttt tctttcagat ccaacccaag taggtacgct
    24781 aaatgccaag caatcgcttc tccttctcta tccgggtcac ttgccaagta aactttttga
    24841 gctttttttg ctgcggcttt caggcttttg atgacatcgc cttttccgcg tatagaaata
    24901 taatggggtt cataattgtt ttcaaaatcg atacccattt tacttttcgg taaatctctt
    24961 atatggccaa cactggccat tactttatag tttcgtccaa gatatttctc aattgtcttg
    25021 gcttttgcag gtgattctac aatcactaaa tatttatacg ccattactga atggactcct
    25081 ttccattgtc gtgttcctac tattaaaaga ggattcatac aaatcgtagc taatcatatc
    25141 cattcagaaa acgtttgtca agaaaagcaa atgaaaagta tggtgttatt ctttaaattc
    25201 tgccaagata tcttcacccg aaaccgtgca aactgcaccg tcttggatca aatgatgaca
    25261 tccggtagaa tgttctgtta aaatatttcc tggaacagaa aaaactgttc ttccaagatc
    25321 caatgcttgc tgtgctgtta tcagagagcc gctttttttc ttggcttcaa agacacatgt
    25381 tcctttagac aaagctgcga taatccgatt tctcattgga aaacaatgct ttttcgggcc
    25441 ggattgagga ggatattcac tcaaaagaag atgatcattt gcaatcacct cttgcaagtc
    25501 acgattttca gcaggataga cttgctctat ccctgttcca atgactgcga ttgtccgtcc
    25561 accatgagag attgctgctt ggtgaccgta tgtatctatc cctttcgcgc agccactgac
    25621 gatcacatat ccagcttcta tcactggttt tagtaaataa taaactgttt ttttggctaa
    25681 agcagaagtg tgtcttccac caacaacgct taacatgtta caagaaagta aagatagatc
    25741 accattataa aacagtacaa ttggcggata ggtcaagtga agcagctgag ggggatattt
    25801 gggatcaaag caagtgataa acgactgttt cgattggatt ttgccccagt tagcgtgaat
    25861 ttcctgccaa cttttgcgaa acagctcccg gtgtttggtt attttaccta tcttaatgat
    25921 gtcttcaaat ccgatttctt tataaggata ctgcatcatc cagttagcaa gtgcccattt
    25981 tccttgacta ctgattcctt ttgtataagc aagtttcaat aaattttctt ctatttgata
    26041 catcataaaa ccgcctttcg tctctctttc taataagata cgtaaaagcg gctgtttttt
    26101 attgtaaata tttttttaca ggtgcaaaac ttttacgatg gattggcgta attccttgtc
    26161 gttcaagtcc ttctaagtgt tcctttgttc cataacctgc attatgcgag aaaccatatc
    26221 caggataaag cacgtcatat tctttcatca attggtctcg atatacttta gcaatgatac
    26281 ttgcagcagc aatcgacaca gatcgtgcat cccctttgat tattttttcc tgctgaatag
    26341 gcaaatccaa ggtcatcgca tcgatcaaca aatagcttgg tacaatggac aatttttcta
    26401 ctgcttggat catcgctaat ttacttgctt cataaatatt cacatcatcg attgtctgct
    26461 gatcaacgat cccgatcccg attgccaggg ctttttcttg aacttgttga taaagaagtt
    26521 ctcgcttttt ctctgataat tgtttggagt cattcagccc taatatgatc tcatttttcg
    26581 gcaagataac tgcagctgcc acgacaggtc cagctaatgg tcctcttcct acttcatcaa
    26641 tgccggcaat ccattggtga ccttgtgcgt acccttgctt ttcaaagcaa aacatttctt
    26701 catatttttc cttaattaat gcttcctttt tcaatcgtcg ttcaaaagaa gccagtgctt
    26761 gctgcacgcc tttgcgtgga tcatttctta atgattctag tgtaggatca tctattctct
    26821 gtatgctttt caatcgttct ttgatacttt ggatggattc agtcatcact tttttgctcc
    26881 atttcttcgc aacgatccag tgtgtatcgt ccaagttttc cttgacgaat atcatgcacg
    26941 actaattcgc tgccacgatc atagtcttct ttatacccac gttttgctgt aatatccatc
    27001 agtaattccg gtgccaattt ttcttcttgc tcgattgata aaccataacg atcgctgatt
    27061 cttcctgaat aatatcgggc aaaaaagtct aatccgtaaa tcgctaaatc atctaaatgg
    27121 acaagctggt ctttgatcgc gcctgtcagc gctagttttt tgccgatttc ttcatcttca
    27181 aatttgggcc ataaaatccc tggtgtgtct aacaattcca actcattacc taaacgaagc
    27241 cactgctgac cttttgttac gccaggctta tttcctgtct gtgcaatttt cttgcctact
    27301 agtcgattca tcaacgtaga tttcccaaca tttggaatac ctaagaccat tgcccgaata
    27361 gcgcgaggct tgatcccccg agcagcatct cttgcccgtt tttctttcaa tgcttcttta
    27421 gccgcaggta ctagaccttt aattccttta ctttcttgtg ccgtgatagc taatgcaaga
    27481 tggccttgtt tacgaaaata ttcttgccac agctttgtct gttgtggatc tgccagatcc
    27541 gctttattca ataaaacaag gcgaggtttt tgctgaatga tctgatctag cattggatta
    27601 cgagaagata aaggtaatcg tgcgtctacc aattcaaata cgatgtccac atatttcatt
    27661 ttttcagaaa cttctcgtcg ggcttttgcc atatgtcccg gaaaccattg gatggtcatt
    27721 gttctctcca ttcctttcat accggaatat tccccggctt tacttgtttt tttctttttc
    27781 aatataaatt cactttttta ttataaagaa attccatcta ttagttaagt gaaagtgaac
    27841 cagtcctcat tctatcacat cttcactttt ttgatgatgc aaatttttat tttggtttct
    27901 atatatgaat ctaaattttt gacagcgttt cctaaataag agatgataga cgaacaaaac
    27961 agaaaggaga cgaaacaaat gaatagtatg gtttttgtta attttcctgt tgctgatgtc
    28021 aatcgttccg ttgcttttta tgaaaagctg ggttttaaaa aaaatgaaga attttctact
    28081 gaagaagctg gttcaatggt atgggatgac actttttgga taatgcttct ctctcatgat
    28141 ttttataaaa aatttataaa agataaagaa atagctgata ataaaaaaac aagtggtgct
    28201 ttgatttcgt ttagtgttga aaatgcggat gaagtaaaaa aatttgctca aattgcaaaa
    28261 gaaaatggtg gtactttcca cgaagtagat atgggaatgc ctgaagaaga aatgttttca
    28321 ttagaagtcc aagatctaga cggaaataca ttagaacccg tatggatgaa gatgtaagca
    28381 gataactaat ctgacgtttt ttataacaaa aaaacaaagg cggatgatac gataagtatt
    28441 ctccgcctct atttttttta ttcgtacaat ctctttttta cagtttataa aagcagtctt
    28501 ctcacccttt ttttaattgt tagatgccag tgtatgtaaa tcaccaatgt gtcatgacac
    28561 attggtgatt ttatggtatc cttttattat taactgacaa aggagagaat attttgaaca
    28621 ctttgattgc aaaagacgtt tttagattgc ccgaaatgaa tgcaagattg ttgtcgacct
    28681 ctgctctttt attagccctt gatgtcatct tatacaagat agcgattggt cctgcttatt
    28741 ttcaaatcac attcggattt ttaagtatgg cgcttatggg ttatctatat ggaccgatct
    28801 gggctggttt gcttgaaatg ttgtcgaaca ctttaaattt cacgatattt ggttcaggaa
    28861 cgtttcaagc tgcttttctt atcacggctt tccttggtgg atttattaat ggcttatttt
    28921 tatatcataa aaagataaac ctgccttccg tcatcgctgc ccaattattg attatgatcc
    28981 cagtcagcct ttttatcaat tcttggctga tgtctgtttt gtttggtgca gatttcaaag
    29041 taatttttct aggtagattc atcaggaata tgatccaaat ccctattcaa ataatggttg
    29101 tttattcatt tatcaaaact ttagaggcta gaaaagtgtt taaacgagct gtttgatttt
    29161 acgtactaag gtagtaaact gattttggat taaacaataa agcgtgcagt ctcaacagac
    29221 tgcacgcttt attgtttctc actggatcca ttttatttca tcaagtggat aatagacaaa
    29281 tcttgcttta cctagaatat cagttttatt gatcgtgcca aacgaacgac tatcttttga
    29341 aactcttcga ttatctccaa gtacaaaata attatcttcc ggaagcttgg attgtcccgt
    29401 tagctcttgt agtgtaaagt ccgtcgtata ggatgcattt gcatgatctg atttcaggtt
    29461 tttagttaga taaggttcct taactacttt cccattgatc ttcaattgat cattttggta
    29521 gctgacattt tctcctggaa gaccgattac tcgtttgata taaatcgtgc catctgctaa
    29581 ttgaaaaaca acaatatcaa atcgtctgat ctccgaaaat ttttccatca caaccatatc
    29641 accttgcttc aacacgtttt ccattgaatt gccttccact ggtaccggga tgaataaaaa
    29701 tccccgcaac atgaacgcaa ggaatacaga aacaagtaag tatttgaata caagccagaa
    29761 gcgattaata tagcgttgtt tttttgtcaa tctctatcaa tccatccctc tttttttcat
    29821 tctttcttta ttataagaaa aatatcaaaa aaatgcgact agatagctga aataaaaata
    29881 attctttcaa ttaggctatt ggacaaaaaa cttttttgat cataaagtac aaaactatct
    29941 cttatatgtc atgattattt tctaaatatt tttcccatgc ttcgtcaaag atatccatgg
    30001 acggcaaata gtcgacattc agttccagat aattagaaat ttcttcgtag ctagagctgt
    30061 gcttaggaaa ttggatgtct ttaccggctt ctgtagcgaa aacagataaa gcatcatgtg
    30121 tgccaccgcc tcgcagtgtc attaaataat gaaaaaagct tcgattcacc cttcgttcct
    30181 ccaattttct tcgataaagg cagcccggcg tgcatggttt cttttaaaat tttcaggatg
    30241 ctttttataa tactcttgat ggtcttcttc tgctagataa aatggttggg ctggttcgat
    30301 ttttgtaacg attggctgtt cgaaacgtcc actttcagct aactgtttct tacttttttc
    30361 agcaatgatc cgctgttctt ctgtcaaata aaagatcacc gggcgataat tgtcaccgcg
    30421 atcctcgaat tgtccgaaag cgtcagttgg atcagtttgc tgccagtaaa gttctactaa
    30481 ttgttcatat gaaatcaatg ttggatcata gataatctcc actgcttctg tatgtccagt
    30541 cgtttggctt tttacttgtt cataagtcgg atctggtaca tgccctccag tatatcctga
    30601 catcactgta tggataccag gaagcgtgtc gaaaggctgg atcatacacc agaagcagcc
    30661 tcctgcaaat attgctcgtt ccattatgat tccccctcta atactactca tccgatgatt
    30721 ttttattttc ttttgtcttg ctttcagtac tgctggtgct agttttgttg gtatttgagt
    30781 tgtttgaagc atcagaggta tcttttggca aatatagact aaaccgaatg tcatcattta
    30841 ctaagtcgat tttatctgct ttaatataca tcccgttttg cattcggaat tgatccaacc
    30901 taagcatgat agtcagatca tctgtattga cttcgatcca atctggtaat ttatagtttc
    30961 tcttgatcat attcatcaca tctttgattg gcaaactcaa tgttccgata gaaagacttc
    31021 tagcttttag ttggacattt ccgttttcca tcacatacgg atcaaaatat aaatagaaat
    31081 cgataggaaa acctagtact tcgaaagtac cattgagtag tgcttcattt tccaaataaa
    31141 atttatattc aatatcacta tctttttgat actcactaag gaaaaagctg atgatttgat
    31201 tgactttttc tttatttgaa ttgatcgtta agacaggatc tccttgtctt tccgtaagag
    31261 ctggtatttc tgggagttct ggttcccgat tagcaaaaat acggctgcct aaaaatacgg
    31321 tcgaaccaag tactacccca agaaggataa ggaaggcgaa tttccagtaa tttgttttct
    31381 ttgttttgtt tgaagatacg tcagatcttg ttttcatgtt tattgatcat cctccttctc
    31441 taaccattgg ttttgtgtct cgatcagctg atctctcact gcatttgcca tcaattgata
    31501 acctaaatta tttggatgga aatgatcttc ttcatagagc acattatttt tgatgccatt
    31561 tagattttct gtcgtgctag ttgtttgatc tgttccttct tctgtgacac ctacttcctg
    31621 attcaatcct tgataaagca aatcattgat tgggataaag tagcagttcg gcattttttt
    31681 tgtcaccgtt tctgttcctt tgttccaatt gttaacaatt gtctgcatgt cagtgatctc
    31741 aggaaagttc aaataaaatg gattgtagat gcctaaaatg taaataggtg cggttttatt
    31801 gagggatcga aattcttcca atagttctgt gacattttcc tgatagtttt tcagtggctt
    31861 tttaaaggaa ttgaccgtta acccgaataa attattttgg ataactttca tcaaatcatt
    31921 accgccaaca gtcagtgtga taaaatcagc tgttttgata ttgtttcgaa tttcttcttt
    31981 tgtttttact cgctttaaga tctggtcact tcgttcacca tttacaccat agttttctgt
    32041 ttcaaccgcg gtcaaattat aacggtcttt tagatcattt gcgacaaggg gaacgaatcc
    32101 tccttgcttg gtttgatctc ctactccttc agtcagcgaa tctccgatag ctatgtagtg
    32161 aagtttttct tttttatttg gatcttctct cacagtagtt actggtttca ttagtgggcc
    32221 agctttcggt atagaagcag ctaaacctgc atagatgatg atcgcaatta ataatgggaa
    32281 caaaacgagg atcaaatgtc gtttcgttaa tttcattttt tcacctcttg tattcgattg
    32341 gttctaatgt tatcaaaaat tttatcaaaa aggtagtctt tatgaaaaaa gaaaacaatt
    32401 attttcctaa ttttataaag aaaaagagaa taagctgcaa gcctttcagg ctgagggctt
    32461 atcctctttt cgactaatct acataataca tgattgcaaa cgcgtcttta ccagtatgcg
    32521 tagcgataac cggattcgta tgcagtacag ggatatgcat gtccgggaat aattcttgca
    32581 aaccctcttt gaattcttgc gccaattctt ttgcatccgc atgtgaaata ccgatttgtc
    32641 gcacatttgg caattctttc agttcatttt tcaaattatt aaaccattta tcgaacgttt
    32701 tctttcctcg ccccttagtt acagggatta attcagaatg ggcgaaattc atcaccacgc
    32761 gtatgttcaa aacactagac agcaaaccag ttgttcggct gattcttcca ccttttacca
    32821 agttatctag tgtggatatt ccgatgtata attttgtatt gtcttgtact tttttgactt
    32881 cttcaaggat tttctctact gttttacctt cttttgctaa ttcagctgct cgaattacct
    32941 gaaaagataa tccttggtca gtggtatcac tgtcgataac cgttactttc gtttttgtta
    33001 gattactggc ttgtcttgct gcttctactg ttccactcaa gccttttgtc atatggatcg
    33061 aaatcacttc actgccgtct tctcctagct catcatacat cgagatgaat tctccaattg
    33121 gtggttggct tgttttgggc agattttttg ctttcgccat catttccata aatctttcgc
    33181 cgtctagttg gtcatcatcg ggatatactg ttccgtcgat catgatggat agcggcatga
    33241 cacgaatgcc taaagcatcg cgtacatttt tttccatcgt acatgaagaa tctgttacga
    33301 tttttacatt tgtcattatt ttctctctct ctttctttaa caagatgcct tattcatgtt
    33361 aaactacatg taagacaatt tctattttta gtataacaga caaataatga tttttcatta
    33421 attacaccta gtaaaggagc cgttttcatg gaaaaaacga aatttaatcg aacgtatgaa
    33481 atattaaatg aagtgtttaa tgcagtcacc catggaattg ggactggatt gagtatcgcg
    33541 ggactagtgg ttcttttagt aaaaggtgcc cacttgcatt cctccctcca tattgtctct
    33601 tatgctatct atggatcgat gatgattctt ttatttttaa cgtctacttt gtttcattca
    33661 ttgattttta caaaagcaaa aaaggtgttc caagtattcg atcatagttc gatttttctt
    33721 ttgattgcag gaagttatac tcctttttgt ttgctgagta tccgaggatg gctaggttgg
    33781 actttatttg tattaatctg gttgtttgcg attcttggca ttatatataa agcgttgacg
    33841 ttgcataaac gggagagagt atcaaaagta tctacgatca tctatatttt tatggggtgg
    33901 ctctgtgtcg tggctgctgt tcctctttat cactctcttg gaatgactgg tgttattttg
    33961 atggtagcag gcgggctttc ttacacgata ggcgcattct tttatagctt gaattctatc
    34021 cgctatatgc atgttgtttg gcatttgttt gttatgttag gtgcaggttt tatgtacttc
    34081 tctgttttat tctttactta ataaaaaaaa taaagctatg gatgggacag ctctaaataa
    34141 cagctagtct atccatagct tttttatatc gtcaattcgc ctgtcccgct ttttacagga
    34201 ttctcattac gctgatacgt ttcaaattga tgcggataac gatttttttc atccatttct
    34261 ccttgactag tattgatcag tgaccaatca gaccagtcaa caggtggaaa ataagcatct
    34321 ccatcgaata catgatggat gactgtacgg tataatatcg tacaaaatgg cagaaattct
    34381 tgataaacag ctgaccctcc agcgatgaaa aagataccgt ctgtttcttt ttccagagca
    34441 agcacgtctt ccacactatg catgacatgc acgccttctg ctttatagga agaatctctc
    34501 gtcaaaacaa tcgtctgacg attcggcaac ggacggctac ccatcccttc gaatgttttt
    34561 cgtcccatca ctagtgtatt tgcttctgtc atttgtttga agaatttcag atcatttggc
    34621 aaacgccaag gaagacggtc ttcttttcca atcaatccgt tttcatcctg tgcccaaatt
    34681 gcagctaaca ttttctcatc ctttctgatt ctaaacagcg ataggagctt tgatcgccgg
    34741 atgtgggtca tatccttcta attggatatc ttccatctca aaatcaaata ctgactgctt
    34801 ttcctgattc aaaactaaag tcggaaatgc gcgtatttct cggctcaatt gttctttcat
    34861 ttgttcgata tgattgatat aaagatgagc atctcctaat gtgtgaacaa attcgccaac
    34921 cgctaaacct gtttcatgtg caattaggtg agtcagcaac gcataacttg caatgttgaa
    34981 cggaacgcct agaaacacat ccccactgcg ttgatacaat tggcagctta gccgtccctc
    35041 ttgaacataa aattgaaaca tcgtatgaca aggaggcaaa gccatgctag gaacatcttc
    35101 cggattccat gcagaaacga tcaatcttct agaatcagga gtctttttga ttgcttcgat
    35161 cacttgtttg atttgatcga taaagccacc gtcttttgtt tcccagtgac gccattgata
    35221 gccgtagatg tgtccaagat cgccgtatgt ttcagcaaag gaatcatcgg ttaggatctt
    35281 ctcgcaaaaa agcttgctct gctttttata ttcttcattg aattcaggat ctgtcaaaca
    35341 tctatggcca aaatcagtca tatctggtcc attatagtct ggacttttga tgtagcgttc
    35401 aaatgcccat tcatcccaaa tatggttttt attttgtaat agatagcgga tattcgtatc
    35461 tccttttaaa aaccaaagca attcgctttt gatcaatcca aaaggtactc gtttcgttgt
    35521 taacaaagga aacccttttt gcagatcaaa ccgcatttgg tatccgaata agcttaatgt
    35581 gcctgtgcct gttctgtctt ctttacgatg cccttcttcc aacaattttc ttcctaagtc
    35641 aagatacgct tgttccatcc tttttcctcc taatcttcta cccattcact caagtattcc
    35701 caccgtgcca ttttttcttc aagttgtgct tctgtttcag atacgtcgtt ttgcaattct
    35761 tgtaatcgag tgaaattatc tccttgatga ttcatctctt cttggagcaa tgagattttt
    35821 tcttcaagtt ctgcaatctc atcttcaatc gtttcccatt ctttttgttc catgtaagat
    35881 aatttcttct tttcagcagg ttccttgctc tgtatttttg gtttcgcttt ttcagcaggc
    35941 tgctgggtct ctttttgctt cagtaaataa ctggacatgt ttccgtaata aagttcttgt
    36001 tttccgtttc cttggaatac taataactta tctgctacct tgtccaagaa ataacggtca
    36061 tgggaaacag tgatcacagc acctggaaaa cttctaaaat aatcttccaa aatcgtcaat
    36121 gttgctatgt ccaaatcatt ggtcggttca tccaataata aaacattagg ttgctgaatc
    36181 agcagtttca acaaaaataa ccggcgtttt tctccgcctg aaagtttgcc tatcagtgtc
    36241 ccatgcataa atcttggaaa taaaaatcgt tcaagtaatt ctgctactcc gatttgactg
    36301 ccatctgctg tttttgcttc ttcagcagct tcttgtaagt aagcaatcat ccgtttatct
    36361 ggtgccattt cttcattttc ttgtgtataa tacgcaagat gaaccgtttc accgatagac
    36421 attgtaccgc tatccagggg aattctgcca gctagaatat taagcaatgt cgattttccc
    36481 gcaccatttt taccagtaat acctagtctt tcccgagatt gaatcaataa atctaaatgt
    36541 tctagcagtg tttggtcatt tatacggtaa ctgccatctt tgatttccag tacttttttc
    36601 cctaaacgtt gtgtcgctag gttcagctct aaatcatctt cttggtttac ttggtacaag
    36661 ttctccttta gatcttcgaa acggttgatc cgtgcttgct gcttggtgcc acgtgcctgt
    36721 acgccagccc tcatccaagc taattcctgt ttataaagcc gcttgcgctt ttcttcttgt
    36781 tctacttcta cacgatcacg ttctgctttt tctaagacgt aagcttcata atttccttta
    36841 tactcgtata attttccaaa tgaaagttcg aagattcggt ttgctactct atctagaaaa
    36901 taacgatcgt gggtaaccat caatagtgcg ccacggtatt gtttcaaata gttttccaac
    36961 cactcgatcg cttcataatc taaatggttt gttggttcgt ctagtaatag taaatcgggt
    37021 tcatcaatca acacttgcgc cagactaact cgttttttct gaccacctga taaagtactg
    37081 atttttttat ctaattcact gattcctaat ttttgcaaaa taatctttgc gttggtatct
    37141 gtagtccaag catcttcttt attcatttgt tcttctgctt tagcataacg tttttgcaca
    37201 tcagggtctt ctccgttttc ccctagttcg atcaatgctt cctcatatgc tcgaacagtt
    37261 tggatcaagg ggctgtttcc ttgaaagact gcttggagaa cagttaattc atcggaaaat
    37321 tcagctgctt gagaaagata accaatacgg taatcagttg gataaaacac tgtttgccga
    37381 tcaccgtcgc tgctatcgat accggcaata atatttaaga gacttgtttt acccgtcccg
    37441 ttcgtcccaa tcaacccgat acggtctttt tcgtggacca aaaaagaaat ctggtcaaac
    37501 aaatcttttt caccataagt ttttttgagg tcagttactt ttaattcttt caacatgttc
    37561 cctccttatc ttggatattg tatcataaaa atagtgaact tcaggaaaat ttttttggta
    37621 tttgttcttt ttgtgttctc aaaaaaagaa caggaccgat tcgatcctgt tctgatatgt
    37681 tttgtttttt ataaaacttg aaaattttta cctttttatt ttattggact cgtttttttt
    37741 gactttgctc gaaacgcaaa tcggaaagaa aagttatcag atggtttgtt catcgtataa
    37801 gattctaaca aattgatatt cctgcgatag ctgttcgcag aaaacgtcgt gattttttca
    37861 gcaacttcgt attcatcaat gatttttctt tcaagataat aacctcttag catttttttc
    37921 gtaaacagac tatcttgctg gatcatccac gaagaaatca aattccccga gttcattttt
    37981 attccttcaa gtttgcgttg ctggataaaa aattgaacca tcgtttcatc ataaatatca
    38041 gtcaagttat ctaaactttc aataatcaac tgagtatttt tttgatatgt ctctctgatt
    38101 tcttggatat cttcatcact aataaatgtc tgacgatcag catatcttct ttgccaaaac
    38161 attttcggat ggagaacgat tcgcattaac ctacggacaa caatgaacca aaagccgata
    38221 aaagacaaaa tctggcttgt gatagaatgt tcgaatcgag aaagaaaacg ggagtaaaaa
    38281 cgataaccat tagaagaaag cttgcccgaa cgataacttt catccaatcc atcccgttcg
    38341 atcgacaaaa tcagtgcttg tatttcttgt acttcttgct tttcatcatc ggttaaatct
    38401 tccaagtaca gatcacgtat ccgttcttga taattttcta tcactgcttc agtggctaaa
    38461 aattcttttg tattacgatc aatttccttt atttcttttc gcaaaacttc aacgacctct
    38521 tctaaaatca tcaaagcatt caaatccgtg cttttcgctt cctctgtttc cgtcagcata
    38581 ggcaagacaa tgatccctat gaccaaagtc actaagatga cacccgccgt cagaaacaaa
    38641 agcaaagacc gctggtaaaa catcatatta ttgattgaaa atggcaaaat aaaaatcgta
    38701 gccaaactga ctgttccctt tacaccgcca aatgttaaga tcaagatctc gttcaactgt
    38761 tttttgaact ttttactgcc atctttgaat acataaaaaa gactgataga aatgaatctg
    38821 atcacaaaaa gcatgacaga gattaatacg ataatcgcta ataaaagacc gtttggatat
    38881 aaaccgtctc cccatactgg cgagaacact tgcgtcaatt cgattcctaa aaatataaat
    38941 actaatgcat tcagtgtaaa agcaatagtt gtccatgtac tgtgggacaa gctcgacaat
    39001 tctgcatcaa acacagagat tttcctaaaa ccagaagctt ggagaatacc tgctgccact
    39061 gcagcaatga ttccagatac atcaaatgcc tctgccaata cataggctag aaaaggaagc
    39121 aaaagttcga tcagcagata ccctgtgaca tcttgtgctg atgctttttc gatcaaatga
    39181 attatttttt gtttgaacca gacaagcaaa aagccgatca aagctccccc aatactagaa
    39241 actactagac tcatccccgc gtttactgct gaaaaagatc cggtgatcag cgcaccaaga
    39301 gcaaactgga aggcagtaac accagatgca tcattcaaaa gaccttctcc ttccaaaata
    39361 tgcatcgcct ttttagggat attcactcgt ccagaaagag aactgactgc taccgcatct
    39421 gtcggaccta acgctgctcc aaaagcaaag caggcagcaa gtgggacact tggcaacagg
    39481 aatgataacg tcgcacctac cccgacaagg gtcaaaatga ctcctccaaa tgccaagaac
    39541 aaaattgtcc cgaaattttt gagaatcgaa gaaatatctg ctttttctcc ttctctaaat
    39601 aacaatggtg caataatcat aactaagaac aattcaggtt caaaatcgat tgattgtccc
    39661 cactctgtca gacctaatac cactccaagg aaaatttgga tcagtggagc tggaatcatt
    39721 ggcaaaatgc ggctagctat gttcgataag gtgatcgtta ctgcaaatac aataactaac
    39781 tcagcaaatt ccatcgtcca tcccctttat tttttatttt gtgtaagcat ttttaaaaat
    39841 tgttcttttt tctttaaaat tttggtttcg actacttcaa cctgtgtttg gttcccctct
    39901 aattcacact gtacacgttt tgactccaaa cgaagcagat catgtttcag acgttccaac
    39961 tgacgataca tcaatatatt tcttttacgc aacttacaaa gtatttgacg gcggtcatcc
    40021 atcgaaaggg cgccatagcg atcatgcaac agtttttttt gttgttcaaa actctctctc
    40081 attttccaga tctcctatac acaaacttcc atcattatag caaagttctt tttggatgaa
    40141 aaatatttca ttgtttcatc gtttattgct agttagaaat ttccataaat ttttcaacgc
    40201 ctcattctat tccaaatagt atcagatatc aagtaaaact taagaaagat aaaagtttct
    40261 gacaaaagga tgtttacttt cccattcctg cctttcttgt tcttttatca caacaaaaaa
    40321 atggggctgc gacaaaaatc ttgtcacagt cccttattct gaataaacgg tgtggaagag
    40381 acagcctatc agtagtaata agacacatct ctttcacagc ctcatttttg cgtaaaaatc
    40441 gatttattcg aaaaattccc cgtatacatc ttctacacgt tgatctggat tgatcacgat
    40501 atacaagtta cctgtttttt cgctgatttt tgttgattga tattgattgt ctaatccttc
    40561 tgttccttta tctagatatg ggaagtacac aagatcaggt gtgccatttc ggtaaaggat
    40621 ttccattcgc tcgatatctt cgtatttcaa tgcgcgatta aacatgccca tttccagtcc
    40681 gcctaggttg atatcatgtg tcgcaactgc gtccccttca tggaagattt cgattttaaa
    40741 tccagcacac ggatggattt caacaaattc actgccattg attcgtccaa aacttgttgt
    40801 gatttgtttg atccaaagat cgccgatatg acggcggttg atcgtccagg tttctccatt
    40861 tctaaaataa aattttagcg ctgtcatttc tttactcatt aaaaagactc ctttatgatg
    40921 taatttattc ttgtactacc tcagtttagc acaaatttat gtaagtggtc acatcttatg
    40981 ttttttcttc taatgacgta tctgcccact ttaagtagcc aacaaagctg agcagataca
    41041 gtctagtgtt ttttaaatca atttttcttt cgataaaaaa tccaaaacct gttttttatc
    41101 attttttatt ctttgttcta cgacggcttt ttcagctaat tgcaaagctt cactaaccca
    41161 cttaccaggt ttttttccag tttcttctag caagatttta ccatctactg ctaattcgct
    41221 taatgacttg ataggtaaac gatcatagga ttctcctacg gcatccatct gaggtgtttc
    41281 gtcaaaatag atcatcaatt cttctacttg cagtgcatgt tttttgccta agaagtacaa
    41341 ttcgaatggt tccaaagctc cagtttctcg ttgtcgtagt ccttggacaa gtgcttgaac
    41401 gtctttaatc atttggttag aacacttcca cgatttcaaa aaggaacgaa tctcattttc
    41461 ttttagatcc agttggctga tcaataatgc ccatgcttgg ctctcttctt tcaatgggac
    41521 ggatggtaat tcagcaaaac gtaaaagtgc ttcgccttct tctttcaaac cgggacagca
    41581 aatgtagcac tctgtttcaa taaatgcatg gattcccgcg gcccgttcat ttcccaagag
    41641 catcttgatg aattccacat ttatacgttc tacagatatt ttacctaata gctcgtggtt
    41701 ttctttgatg gcgtagaagg tttcttcctc taatttgaac cctagttgac tgacaaaacg
    41761 caacccgcgc atcattcgta aggcatcttc atggaaccgc tcatgaggat tgcctacagc
    41821 tcgcaagatc tgttcttcta gatcttgcag accatcgaac aaatcgatga tttctccatc
    41881 ttctttcaaa gcaaacgcat tgatcgtaaa atctcttctt ttcaaatctt ctgccaacga
    41941 acgaacgaat tcgacatgat ctggtctacg gtagtcctga taagttgatt ccgtccgaaa
    42001 tgtggtgatc tcatattgtt catcattttc taataccaac acagttccat gttcgatccc
    42061 aatatctatc gtttttggaa aaagctgttt gatttcttct ggaaatgcgc ttgttgcgat
    42121 atccacatca tggattggct ggttcagcaa gatatcacga acacttccac ctacaaaata
    42181 agcttcatat cctgcattct ctaatttttt cagaactgga attgcctttt ggtattccag
    42241 aggtaattgt tttaatttca tttttccaac ctttcctgcg tgtttcttgt cctgagataa
    42301 agactcagtt gcatggtgac tgtttgacaa gtccgtttca gagtcttcaa ggccaggacc
    42361 acttcatctt gtttactcgt ccttacgctc aaagcgatta tggtctcttt gattgaattt
    42421 ttccatgttt ttttcaaaaa cttcagttaa gtcgatattc aatgaattag ccatgatcat
    42481 cgtaacgaat aaaacatcac caagttcttc tgctaccgag ttaactggtt cagaagcctt
    42541 tttgcttttt tctccataag tgtggttgac ttctcttgcc aattcaccta cttcttcagt
    42601 taatcgagcc atctgagcta atggagagaa ataccccacc ttgaattgct ggatatattc
    42661 atccacttcc tgctgcatac tgtataaaga acgtttttta tccatctttt tccctccaaa
    42721 actatcttac caaaacttta acgcaaatcc tagataagag caattgagaa aatcgaattt
    42781 ccatgctaca tttaggaagg aatttctaaa tggaaattct gtgaaaatac ataattgaaa
    42841 gctggaattc atatggtcaa acgaaaaaca gtaaaagaag ttctttttat cattatagga
    42901 acaagtattt atgcatttgg tcttgtttac ttgaacatcg ctaatcattt agcagaaggg
    42961 ggagtctcag ggatcacctt gatcttaaga gctttatttg gtattgatcc agcttatacg
    43021 actttattaa tcaacatccc attgatttta ctaggaggaa agatcctcgg taaacgttcc
    43081 ttggcctata ctgttctagg tacactttct ttgtcattct ttttatggat ctggcaaagg
    43141 gttccactag aaatcaattt acagcatgat ctactgatcg tttctctact tgcgggttta
    43201 gttgcaggtg taggaagcgg gatcgtctac cgcatgggtg gaacaacagg cggcagtgat
    43261 attatcgctc ggattttaga acaaaattac ggcatcacta tggggcgttc cctattggca
    43321 tttgatatca tcgtcttact tgcttcatta acctatatcg acttgaaacg tatgatgtat
    43381 acgttgattg tttcgtatgt tttcagtcgt gtgattgatt cgatcttaga tggcggatat
    43441 tctgcaaaag gtctacttgt cgtctccaac aaaagcgaag agattgcacc actactgatg
    43501 accggtctgc aaagaggtgt gactttcctt tctggagaag gcgcattctc tggtaattca
    43561 aaaaaaatca tctatatggt cgttagctct agtgaattaa ctgaagctaa gaggatcgtc
    43621 cacgagattg acgaaaaagc tttcctttca atcatcaatg tgcatgaagt ggaagggcaa
    43681 ggctttactt atttgaagcc acagacaaga ttactgaaaa aactataaga aaaaagacaa
    43741 agataggcta tgaggaaaga ttccacatcc agctgacctt tgtctttttt agtactatac
    43801 tatttttaac taattgttat gttcacttac ccggttactt ttcttttgtg tctgcaaaag
    43861 gatctttcgg ttaacttcat cagataattt caaaaaagca tccctatctc catcttctag
    43921 tgcttgatcg atttgagcat aaagcatttt tagttttgcc tcttcttctt tttcttgaaa
    43981 atatcgttca attaattcag tatcttcttc actgactgaa gaatcccaag aagaaaatgg
    44041 attgtcttct aagatagata gatattctct tgtctgccaa gcattttcga atatacattc
    44101 cacatataaa ggctttttcc aattcaaacg gatttcatga aaaatctggt ctgtgtccgt
    44161 aaattctttt ccctgcaaaa acaatttcat gggttcccct tcaacagata gatcactgat
    44221 ctgcagtcct cgatccgcaa aattcgcccc ttcaacaaag tggacattgc ttaaaatcgc
    44281 ttcatgattg gcaagataat tcaagatcca aaaaacttct cttcggctaa aagatacatg
    44341 attgaccatc cacgttaaaa aattcttctt ttcccgtaca tcaattagca tttttctcac
    44401 ctctctgtta ggtcatctaa taatgacagt gcctccatat ctcctggttc aagtgttaaa
    44461 taataagtca ataattcttg cgctcttttc agctgccctt cttctcttaa gaaaaaggca
    44521 tactctttca agaaatctgg ttcatgtgat agttcatgat aagcctgttc atagtgtaca
    44581 gctgcaacgg taaagtcttc taattcatta taagcatggg ctaggttcca ttctgcataa
    44641 ggattagcag tttcttccat ctgattgatc acttgtatca catcttcata tctttcatca
    44701 tccaagtaaa gattacttaa agtaagtaat gtttcatctt gtttgtctcc tacttctaaa
    44761 gctttcagta aaagttcttc tgctcgctgt ctatcatgca gacggaaagc attttccgag
    44821 gccaggtgat aaagttcgac ttggtaagga ttttctttga ttccttcttc taacactgtt
    44881 tgtgcttctt caatcatttc ttcttcttga agagcttgtc ctagatatag atataacgat
    44941 tgataatgcg gattaacttc tcgaagttct tccagatagt tgattgcctg ctgattttct
    45001 ttcaattgaa gattcgtaaa agctagctgg aacagtcgat catctgtcct ttcttctttc
    45061 agtgcttctt ctaaaggtac gatagctgct tcgaattctc cttgcatact taaggattgt
    45121 cctaaacgtt cctgcatcga gattcctgaa atttcattta tcccgcttgc caacaatgtt
    45181 tcataaacca ctgctgcctc tttgaaacga tcaactgaaa aatacaattc agctagcgca
    45241 aactggatca atggttcatc aggaagcaac ccagctgctt ctttcaattt tgcttcactc
    45301 acttcaggga ttccgataac ttggtacaaa tcagctatcg ccaataagct ttgcggatag
    45361 taatcacttt gcttatccac tttttccagg tattcaaaag ctaagtcgat ctcattatct
    45421 tcgattgcaa tttctgctaa cgggatgttc agtccgtcat tttctgggta ttggttgatc
    45481 agttgttcaa aaatctgttt tgcttcgatc aaaaagccaa tagccagcaa ttcttcgcct
    45541 agttcagcta atatatctgc tgggtcttct tttaatgctt tttctaaaaa gatttgtgct
    45601 tgtgacaagt cttcatctga taaagcttgc aacatttttt cgctatttgt attcatttca
    45661 attccctctt atctcactga cgatttgttg aaataccgtc attgttctca ataattcttt
    45721 ttcgtttgga tgttggtatc cgtctattat acctattttt tctaaaacaa gtattttttt
    45781 gcttttttct gccaaaaaaa gaactttttc caaatactcc atctttgaaa tctgttccag
    45841 ccagtggatt ggatagccaa gcaagtccag ccacacaaaa aagtttttta gttggaaatc
    45901 aaaaggatat gcttggagat tccacacgaa atgaaacatc attcctaaaa attttttcat
    45961 actagcagaa agtagatggc cattttcggt cagataaaaa gcttgttcaa acagtttacc
    46021 ataatttgac aggttttctg cctctaattg ataaaaatag atcatttctt cgatcatccc
    46081 ggcaaacggc cgagtcgtta attgcttttt atttggatag tttttataca gattttgcaa
    46141 aaaacgatga tcacaaatca ttccgcaaca gataaaagtc aaaaagtcaa tctgctttcc
    46201 gtccacctgc cgctcgctga ttgtttgatc ataaacgatg cgttctggca gattgatggt
    46261 ttgcagcact acacgatttg gtagctgcaa aatcgtccgc tgcggtgtta atgctcggct
    46321 tagagaacgg atcgatacag ggatcatcca tagtgatgaa tcaagggttg tatgtttttg
    46381 aaaaaaacct gccagatcca tgatcccttc atttccaaat ccgatgataa acatggattg
    46441 attttcggga tagcgtttga cgaaatcaag caggttcttt aattctgtta aatgattgca
    46501 ttgagtgttg gcacaaatat accagtcatt gtttgcttga tttgcaaata acgggttgat
    46561 cttatctgca aacaaatcat aatagcgctg attcgttaaa aataagattg gctgatcaat
    46621 ggaaagttct cttagctgac tgactagcgt ttctccgtaa aacactttcg tcaacgcatc
    46681 ttctacttga taatcaagtt ccatacgcat ccctcctctt cttattttta ttttaccatt
    46741 agtaagcaaa ttctagaaaa gataagagca aagctcacct cataacttgc aaataaataa
    46801 aaattaaata aaatacatgt gttttctatg tatgacatac ggattcggaa tctctgtctc
    46861 ttatttattc cttttctgct aggaaataaa aagaaaagca cttgtagccc tagagccaca
    46921 agcgcttttt caactgtctt atttaacagc gtcttttaat gctttacctg gtttgaatgc
    46981 aggtactttg ctagctggaa tttcgatttc ttcaccagtt tgtgggttac gacctttgcg
    47041 agctgcgcgt tcgcgaactt caaagttacc aaaaccgatt aattgaactt tttcgccgtt
    47101 agctaaagca tcttgaatag ttgaaaatac agcatctact gctgcagttg catctttttt
    47161 agttaaatca gtagctgaag caactttttc gattaattct gctttgtttg ccatggatag
    47221 attcacctcc tccacaagag atatgataga aatatcattt agttaaaact ttctgagtgg
    47281 tccagaagtt taaaaagaaa tattgattaa cttcaatatt ctgttatcac gatatcatat
    47341 gaaccgctta atagcaaggt ttttaccaat tttcacatgc tttttttagt catagaaacg
    47401 taacaaacga aactgtcttt ttcttttgaa aaaaaaacgt cgatatggta aaatgcctat
    47461 tttctcctac gagggataat tcggatggac gtcccttcaa aagtaaatgc ttttctgatt
    47521 tgattttcta agaaacgttc ataagaaaaa tgcatcaatt cctcttcatt gacaaaaacg
    47581 acaaatgtag gtggtttgac agcgacttgt gtagcataaa aaatcttcag gcgttttcct
    47641 ttatctgttg gtgttggatt gattgctaca gcatccataa tgatatcatt caacacagcc
    47701 gaagagatac gaagattctg gttcatactt acttgttcga tcatttcagg cagacgttcc
    47761 aaccgttgtt tagtcactgc agaaacaaaa acgattggag cgtaatcaag ataccggaat
    47821 tcatcacgaa tctcttgttc gaaatcacgc attgtattgg tttctttctt tacaagatcc
    47881 catttattca cgacgataat aattccacga ccagcttcat gggcgtaacc cgctactcgc
    47941 ttgtcttgtt cccgaatccc ttcttcagca ttcagtacca tcaaaacgat atcagaacgg
    48001 tcaatggcac gcattgcacg catcacgctg tatttttcag tattttcgta tacttttccg
    48061 cgtttacgca taccagctgt atctatcatt aagaatttct gaccactttc agaaacgaag
    48121 tgggtgtcga ttgcgtctcg ggtcgttcct tcaatatccg aaacaattac acgttcttct
    48181 cctaaaatcg cattgatcaa agaggattta ccaacatttg ggcgaccaat caaactaaac
    48241 ttaatcgtgt cgtcgtcctc ttcttcagtc tcttcagaga aatacttcac tgcttcatct
    48301 aaaatatcac caatccctag tccatggctt cctgaaacag gataaggatc gcctagccct
    48361 aaggaataaa attcatagat atcgtttctc atttctggat tatctacttt attgactgct
    48421 aaaatcactg gtttattact tctgtataaa attttcgcaa ccatctcatc tgcgtctgtc
    48481 acgccttcgc gtccgctgac tacacaaatg attacatctg cttcttcaat cgcgatttct
    48541 gcttgatgct tgatctgatc cataaaaggt tcatcgccaa gatcaattcc gcctgtgtcg
    48601 atcacgctga attctcgacc taaccattca ccttttgcat aaatacgatc acgagttact
    48661 ccaggagtat cttcgacaat cgagatacgc tcaccagcga tgcggttaaa aatcgtagac
    48721 ttaccgacgt tcgggcggcc aacgattgca attgttggat ttgccatatt atttcctcct
    48781 cacaagtttc caatctcact tagtttaacg aatgcattgc caactttcaa gcatagattg
    48841 tcaattaaac gaagtatttt tattccttat tgaaatacta atcgcttttc aaaagaaaaa
    48901 ggctctatac tttttgatgt tggtggatga caccagcatc ttttttattt tccaaaacat
    48961 atagaaaaga caccttgaa
//
LOCUS       DACEUF010000007        47697 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.008, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000007 DACEUF010000000
VERSION     DACEUF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.008"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            101..544
                     /locus_tag="IXG20_000452"
     CDS             101..544
                     /locus_tag="IXG20_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1608670.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(550..1734)
                     /locus_tag="IXG20_000453"
     CDS             complement(550..1734)
                     /locus_tag="IXG20_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ1608671.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2002..2700
                     /locus_tag="IXG20_000454"
     CDS             2002..2700
                     /locus_tag="IXG20_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ1608672.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2707..3384
                     /gene="nth"
                     /locus_tag="IXG20_000455"
     CDS             2707..3384
                     /gene="nth"
                     /locus_tag="IXG20_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ1608673.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3510..4043)
                     /locus_tag="IXG20_000456"
     CDS             complement(3510..4043)
                     /locus_tag="IXG20_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608674.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4340..6721)
                     /locus_tag="IXG20_000457"
     CDS             complement(4340..6721)
                     /locus_tag="IXG20_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1608675.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6711..7358)
                     /gene="recU"
                     /locus_tag="IXG20_000458"
     CDS             complement(6711..7358)
                     /gene="recU"
                     /locus_tag="IXG20_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ1608676.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7422..7961
                     /locus_tag="IXG20_000459"
     CDS             7422..7961
                     /locus_tag="IXG20_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ1608677.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8051..8470
                     /gene="gpsB"
                     /locus_tag="IXG20_000460"
     CDS             8051..8470
                     /gene="gpsB"
                     /locus_tag="IXG20_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ1608678.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8492..8859
                     /gene="rnpB"
                     /locus_tag="IXG20_000461"
     ncRNA           8492..8859
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IXG20_000461"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9068..10234
                     /locus_tag="IXG20_000462"
     CDS             9068..10234
                     /locus_tag="IXG20_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ1608679.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10280..11776
                     /locus_tag="IXG20_000463"
     CDS             10280..11776
                     /locus_tag="IXG20_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ1608680.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11857..12540
                     /locus_tag="IXG20_000464"
     CDS             11857..12540
                     /locus_tag="IXG20_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1608681.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            13185..14375
                     /locus_tag="IXG20_000465"
     CDS             13185..14375
                     /locus_tag="IXG20_000465"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ1608682.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            14477..15430
                     /locus_tag="IXG20_000466"
     CDS             14477..15430
                     /locus_tag="IXG20_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ1608683.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            15499..16848
                     /locus_tag="IXG20_000467"
     CDS             15499..16848
                     /locus_tag="IXG20_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1608684.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(17031..17354)
                     /locus_tag="IXG20_000468"
     CDS             complement(17031..17354)
                     /locus_tag="IXG20_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608685.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            17483..18646
                     /locus_tag="IXG20_000469"
     CDS             17483..18646
                     /locus_tag="IXG20_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608686.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            18716..20065
                     /locus_tag="IXG20_000470"
     CDS             18716..20065
                     /locus_tag="IXG20_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1608687.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMVFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(20110..20541)
                     /locus_tag="IXG20_000471"
     CDS             complement(20110..20541)
                     /locus_tag="IXG20_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1608688.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            20929..23571
                     /gene="alaS"
                     /locus_tag="IXG20_000472"
     CDS             20929..23571
                     /gene="alaS"
                     /locus_tag="IXG20_000472"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ1608689.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            23771..24367
                     /locus_tag="IXG20_000473"
     CDS             23771..24367
                     /locus_tag="IXG20_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1608690.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            24489..25637
                     /locus_tag="IXG20_000474"
     CDS             24489..25637
                     /locus_tag="IXG20_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1608691.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(25675..26088)
                     /locus_tag="IXG20_000475"
     CDS             complement(25675..26088)
                     /locus_tag="IXG20_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ1608692.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            26167..26598
                     /locus_tag="IXG20_000476"
     CDS             26167..26598
                     /locus_tag="IXG20_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ1608693.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(26697..26930)
                     /locus_tag="IXG20_000477"
     CDS             complement(26697..26930)
                     /locus_tag="IXG20_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ1608694.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            27113..28783
                     /locus_tag="IXG20_000478"
     CDS             27113..28783
                     /locus_tag="IXG20_000478"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ1608695.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            28930..29316
                     /locus_tag="IXG20_000479"
     CDS             28930..29316
                     /locus_tag="IXG20_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608696.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            29303..30001
                     /locus_tag="IXG20_000480"
     CDS             29303..30001
                     /locus_tag="IXG20_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1608697.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            30006..30470
                     /locus_tag="IXG20_000481"
     CDS             30006..30470
                     /locus_tag="IXG20_000481"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ1608698.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            30467..31378
                     /locus_tag="IXG20_000482"
     CDS             30467..31378
                     /locus_tag="IXG20_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1608699.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            31613..32152
                     /gene="pyrR"
                     /locus_tag="IXG20_000483"
     CDS             31613..32152
                     /gene="pyrR"
                     /locus_tag="IXG20_000483"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ1608700.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            32166..33449
                     /locus_tag="IXG20_000484"
     CDS             32166..33449
                     /locus_tag="IXG20_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ1608701.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            33462..34388
                     /locus_tag="IXG20_000485"
     CDS             33462..34388
                     /locus_tag="IXG20_000485"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ1608702.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            34407..35687
                     /locus_tag="IXG20_000486"
     CDS             34407..35687
                     /locus_tag="IXG20_000486"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ1608703.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            35692..36768
                     /gene="carA"
                     /locus_tag="IXG20_000487"
     CDS             35692..36768
                     /gene="carA"
                     /locus_tag="IXG20_000487"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ1608704.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            36773..39958
                     /gene="carB"
                     /locus_tag="IXG20_000488"
     CDS             36773..39958
                     /gene="carB"
                     /locus_tag="IXG20_000488"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ1608705.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERTGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            40012..40788
                     /locus_tag="IXG20_000489"
     CDS             40012..40788
                     /locus_tag="IXG20_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ1608706.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQSSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            40785..41717
                     /locus_tag="IXG20_000490"
     CDS             40785..41717
                     /locus_tag="IXG20_000490"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ1608707.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            41714..42430
                     /gene="pyrF"
                     /locus_tag="IXG20_000491"
     CDS             41714..42430
                     /gene="pyrF"
                     /locus_tag="IXG20_000491"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ1608708.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            42437..43066
                     /locus_tag="IXG20_000492"
     CDS             42437..43066
                     /locus_tag="IXG20_000492"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ1608709.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            43054..43368
                     /locus_tag="IXG20_000493"
     CDS             43054..43368
                     /locus_tag="IXG20_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608710.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            43406..44047
                     /locus_tag="IXG20_000494"
     CDS             43406..44047
                     /locus_tag="IXG20_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1608711.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            44078..44782
                     /locus_tag="IXG20_000495"
     CDS             44078..44782
                     /locus_tag="IXG20_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ1608712.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            44779..45681
                     /locus_tag="IXG20_000496"
     CDS             44779..45681
                     /locus_tag="IXG20_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1608713.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(45839..47545)
                     /locus_tag="IXG20_000497"
     CDS             complement(45839..47545)
                     /locus_tag="IXG20_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ1608714.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        15521 a         8349 c        10269 g        13558 t
ORIGIN      
        1 tttttcttgg tttcagattg ggttttccta caaaatattt ttatctttgt tgaataggaa
       61 acaaaaaaac aatataatga aagaaaaaga aggtgtagaa atggataaaa gacaactgct
      121 aatagaagaa tatctggatt tagctaatga gattattcat cgtaataaac cagaaatgga
      181 aaaagaattt gcaggattta ctctaaatga actagaagtg attgaacata tcgggaaaat
      241 ccctgatcct aacgtgacaa aattagcgaa tgctagttac atgactagag gagccatcag
      301 taagctaaca aaaaaactga tagctagagg gatcataaac acttatcaaa gcgaagagaa
      361 taaaaaagaa atctattttc aattgacgcc aatcggacaa aaaataagga atagacacga
      421 gcaactccat caaaaatggg tacagcgaga tgctactgta tttgaaaata tgtcaaaaga
      481 agaatttgac acagtatttc gttttatcgg tcgatacaga gaatttatac gaagtagcaa
      541 atgaagctac tattttttat tgtagtgata ataggcaaaa atcataagca aaaatccgaa
      601 taccgtcgga agaaatggtg ctgcagcata aagctgtcct gcgattagcg ggccgatgac
      661 gcgcgataga gattgtatac tttgggtacc gcccattaca agaccttgct ggttatctga
      721 gacagaattg gatagttggc cattgaatac aggagtaaaa atagaatcac cgaaaccata
      781 acagataatc ccaaataaaa acaaaatcgg cagtagaaga attccagata atgtgatgaa
      841 tagataggcg aggatttcac tgaacatccc tattcttgtg atctgctgtt ctgaaaattt
      901 tttgagcaat cgaggcataa tgaacaattg cgagacagta tccaagattc caatcaaaga
      961 gaagcttaaa ccaaccaacc cagccttcca ctgaaaggcg tcaatcgaaa attgagaaaa
     1021 gatagattga agagagccag ctgccaacca aacagcaaaa cctgctgtca ataaaggaat
     1081 aacggatgaa atcttgaata attgttgtaa ttggtgaaaa ggtcttacat gggagaaact
     1141 taaatctact gaacgttttt ttattggaag gctttctagc ataaatgtgt agccataaac
     1201 ggcatttaag aaagtaaaga gtgctccaat aaatatagga acagaattcc ccaactcagc
     1261 taatagaccc cctataatag gacccagcgt tgttcctata ccgactaaag cacccatcca
     1321 tccaaaaact ttggttcttt cattaggttc tgtgatgtcg gcaaagtaag catataaggt
     1381 ggagatttct cctgccgtca atccttcaat gatacgacca aggaacaaca tccatatcga
     1441 atttcctaaa ccaaaaagca aatatccgat acttgatcct aataaactga taataaggat
     1501 cggatgacgc ccaaaatgat cacttaagct accgagaatg ggggcagata agaaggcagc
     1561 tagagcataa acagacatga gcaaagtgat atagaaagct tgctgatgta tggagtgaga
     1621 ataaggcagc gttaaaaacg gaagtacagg actgacgata gtaaatccta atccagtgaa
     1681 taagacagag acgtatccaa aaactttaga atgtttattt gtgaaactgt acatgtaaaa
     1741 actcctttat gcaataatgt taacttggaa acaaaaatag tgtatagtaa ttttgtttcc
     1801 ttgtcaacat tctaaggaac agcgaaatct acttaattta tttattccga aaagtgaaga
     1861 tacgcatcaa aaacatgaac aaacaaaaat caagtagacg acattgtaaa tctgaccgat
     1921 cagtgaagta aatagaaaag aaacctgttc ttgcttccca tgtttttttt acgtatactg
     1981 tgattgagcg gaggagaaat catgttgaat ttagaaaatt acttaagcgc tggacaaaca
     2041 gtcatatcta atcttttact taaaaattat cataggatcg gtctacaatc tgaagaattt
     2101 ttatttgttc tacagttgca tatggcacag cttgaaggag atactttccc tgatttgcag
     2161 atgattgcta tggatatggg aatcaaacaa gatcagattt ttcaaatact ggatcgtctc
     2221 gtaacaaatg gatttatcaa gatagaaacg acgattgtca acggtaaaaa agcagatcga
     2281 tataatcttt accccatcta tgatctatta ggtgagtatt taaaaacaca agaaaaaaaa
     2341 tacgaagaaa aaacacaaga aaaagaaatc cagtcagtgt atcaattatt tgaacaagaa
     2401 tttggacgcc cgctttcttc gatcgagttc cagcggatcg gacaatggct agaagaagac
     2461 cattatcagc cggaaatttt gaagctagca ctgcgggaag ccgtattaaa tcaggcttat
     2521 agttttaatt acgttgaccg cattttgcta tcgtgggaaa gaaaaaatct acgtacaaaa
     2581 caacaagtgg aagaagacca aaaacgaaga aaacagcagc ttcttcaaag agaagtagaa
     2641 cagtcagctc aacaagaaga attgccgaaa gtctcactga aaaattggtt ggaggagtag
     2701 cggaatatgt taagcaaaca aaaaacaatg gaagctctag aaacgatgta tggaatgttt
     2761 cccgaagcac atggagaact caagcacaat aatccatttg agttattgat tgcagtgatc
     2821 ttaagtgccc aggcgacaga tgtatcagtg aataaagcaa cgccagattt gtttgcctct
     2881 tttccgacac ccgatgcgtt agcagaagct tcaatcgatg aaatcattct taaaataaaa
     2941 acgattggtt tgtatcgaaa taaagcaaaa aacatcaaag cgtgtgcaca gcagctgatt
     3001 gaacgtttcg atgggcaagt gccaacgtca agagaagaat tgatgtctct tcctggtgta
     3061 ggcagaaaga cagcaaatgt cgtattgggg gatgcattcg ggataccagc aatcgcagta
     3121 gatacccacg tggaacgtgt ctcaaaacgc ctgaggatct gcaaactaga tgctacggtc
     3181 atggaagtag aagaaacact gatgcggaaa gtaccacaag aattatgggt aaagacacat
     3241 catacactga ttttttttgg tagatatcat tgcacggcaa gaaatcccaa gtgcgaggta
     3301 tgtcctttgc tatcaatatg tcaagatggg aaaaatcgga tgcgtctaaa agaaaaggcg
     3361 ctaaaaaaga aaccacgcct gtagacgtgg tttctttaat tgcaaataaa tgtatgaatt
     3421 atcttgttat tatggagaaa agctgtttcg tttatctttt ttgcatgaaa aagtaaatgt
     3481 acatactcag tagctaggat ggtaacttat tacaatttat tgcgataaaa ataaaagttg
     3541 ttatttttat tgggaaaata cccgatgtag aaaataatgc cttccaatat acctataaaa
     3601 taagtccagt attccaaagg attatataaa taagtgacgt atctatctaa aggtgtaata
     3661 aaaatgataa gtgataataa aaaaatacca tttataacta aacataaact aatggatttg
     3721 ggcggtttca ggctcatcag aaaaatatag attctggtga acaacaacca taaaaaagtg
     3781 agaatcattg catgagagct tctttccata aattttcctt taagagtagt gcttaaaagt
     3841 acagaaaaaa taataaatgc aaggatagga gcaattaaag taaatttatt taataataaa
     3901 caaaattttt cagctttttt attcttggaa taacgaggta ttgttttcga taaaaaaaga
     3961 tggtgcagac aaattatgat aattaagcat actatcagat aactcaaaat gaagccttct
     4021 ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac aatgggatta
     4081 ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat tataaacaag
     4141 taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa
     4201 atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg
     4261 gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt
     4321 agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat
     4381 ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag
     4441 atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg
     4501 gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac
     4561 tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat
     4621 agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat
     4681 ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat
     4741 ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg
     4801 acgattctga agtgacaggt gtcatttttt tattgtaacc tgtccacacg gaaatcgaat
     4861 aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt
     4921 ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag
     4981 cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca
     5041 tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc
     5101 cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga
     5161 aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt
     5221 cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat
     5281 tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa
     5341 cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc
     5401 agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc
     5461 cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag
     5521 aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga
     5581 tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg
     5641 caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata
     5701 gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat
     5761 tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct
     5821 tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag
     5881 cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat
     5941 ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag
     6001 gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt
     6061 acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt
     6121 agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt
     6181 cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga
     6241 tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg
     6301 cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta
     6361 ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct
     6421 tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg
     6481 tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc
     6541 ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa
     6601 ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca
     6661 cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca
     6721 ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa
     6781 ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa
     6841 ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta
     6901 ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt
     6961 tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa
     7021 gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca
     7081 ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc
     7141 ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac
     7201 ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg catcccccga
     7261 ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc
     7321 ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca
     7381 cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa
     7441 tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa
     7501 tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg
     7561 aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg
     7621 aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg
     7681 gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt
     7741 atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc
     7801 gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa
     7861 aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc
     7921 ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt
     7981 atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag
     8041 aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa
     8101 aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa
     8161 agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag
     8221 tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac
     8281 agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct
     8341 tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca
     8401 acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg
     8461 tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat
     8521 caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg
     8581 aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct
     8641 atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg
     8701 gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg
     8761 aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt
     8821 ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt
     8881 aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc
     8941 aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga
     9001 atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag
     9061 attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga
     9121 agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg
     9181 tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc
     9241 agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga
     9301 aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc
     9361 tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa
     9421 agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga
     9481 aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct
     9541 ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc
     9601 attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc
     9661 attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca
     9721 taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca
     9781 agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt
     9841 agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga
     9901 agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac
     9961 ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga
    10021 agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg
    10081 gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac
    10141 caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac
    10201 tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg
    10261 atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa
    10321 tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa
    10381 agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa
    10441 aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc
    10501 ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt
    10561 accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca
    10621 aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga
    10681 attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt
    10741 gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct
    10801 taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac
    10861 agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat
    10921 cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat
    10981 gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa
    11041 aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga
    11101 tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca
    11161 atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc
    11221 tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg
    11281 ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc
    11341 tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt
    11401 tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac
    11461 aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt
    11521 tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga
    11581 aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc
    11641 agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac
    11701 acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt
    11761 atatcaggta aaataaaata caatttacaa agatagctga aattcatcta atctatagat
    11821 gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct
    11881 gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa
    11941 agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg
    12001 acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac
    12061 gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc
    12121 tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc
    12181 gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca
    12241 atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct
    12301 gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc
    12361 aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat
    12421 gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa
    12481 tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga
    12541 tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt
    12601 acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag
    12661 gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat
    12721 tggcaggaga acaccgttta ttcggaagaa taggactgtg acaagacttt tgtcacagtc
    12781 cttttttcga attcaacaag taaaactttt aagaaatatt cagagagtaa aattaaaaat
    12841 tgctgataaa cgaagatgga aattttatgt atttttattg gtctatgcaa taaataaatt
    12901 aaatattcag aatacaattg attttaacga aagggcttct atcaatggtt gtgttgtatt
    12961 tatctgccca ggtagagtaa aatagatcat tatctaaaag gtaattgttc atactatata
    13021 agtcatttga aagtaaaaag atactgtaaa taaaaaatgt ggttaaataa ttctcataat
    13081 cagtgtaagc gttatcaaaa atatagcata cttgctttgt agatgaatga cacgatatat
    13141 gtttggtatg ttctgcaaga cagatgaata tggaggtgga gacaatgtct tccaaagaac
    13201 taacaatgta tttagagaaa gaattggatc aactgaaaga aaaggggtta tacaatacga
    13261 tcgacatact agaaagtgaa aatggtgctt gtatcatcgt agacggaaaa aagatgatca
    13321 atctagcatc caacaattat cttggatttg ccaatcgcga agaattgaaa aaagcatgta
    13381 ttgaagcaac cgaaacttat ggtgtaggag caggagcagt ccgaacgatc aatggttcgc
    13441 tgaaaatcca tcagcaatta gaagaaaaaa tcgcagaatt caaagggaca gaagcagcaa
    13501 tcgcctttca atctggattc aactgcaata tgggcgctat ctctgcagta atgacaaaag
    13561 aagatgctat cttatcggat gaattaaacc atgcatctat tattgacggt tgccgcttat
    13621 ccggagcaaa aatcatccga attaaacatc aagatatgaa agaccttgag aaaaaagcaa
    13681 aggaagcgat agaaagtaag aaatataaaa aaatcatgta tatcacagat ggtgttttct
    13741 ccatggatgg agacattgca cgattgccgg aaattattcc gattgttgaa aaatatggac
    13801 tgatcaccta cgtcgatgat gcgcacggct caggcgtaac tggaaaaggt gcaggaaccg
    13861 tcaagcattt tggattaagc gacaaaatcg atatgcaaat gggcacgtta tcgaaagcta
    13921 taggtgttgt tgggggttat gtagctggat cgaaaacact aatcgactgg ttgaaagcta
    13981 gaagtcggcc attcctattt tctacttcgc taactccagg tgcagctgca gctgcattgg
    14041 cttcgattac tttgatgcaa gaacatccgg aacttgtcga aaaagtttgg gaaaatgcta
    14101 attattttaa agaagaattg aaaaaagtag gctataacat cggtatgtct gaaacaccta
    14161 ttactccagt cattttggga gacgaaaaag tgactcagac tttctctaag aagctgatcg
    14221 aacatgggat ttatgctaaa ccaatcgttt atccaactgt gcctcttggg acaggcagga
    14281 tcagaaatat gccgactgcg gaacatacga gggaaatgct tgatgaagca gtagcagttt
    14341 atcaaaaaat aggaaaagaa atggaaatta tttaactaaa tttagttttt tcgtctattt
    14401 aaaattagag aacatgttca cgcatatatt gttaatcatc tgaagatagt agtaaaaaag
    14461 atcagggagg atcatcatga aaaaagtatt ggtgacagga tgtttaggac aaattggttc
    14521 agagttgatc acacggctac gtcaagaact aggcagtgaa aatgtcattg cgacagatat
    14581 acgcatgcct gaagtcattg agaaaaacga tatctttgag atcctagatg taatggatta
    14641 cgaaaaaatg cgcagcatag cagaacaata tcaagtggac acattgattc atttggctgc
    14701 tcttttgtca gctgttgcag aagcaaaacc acaattagcc tggaatttga acgtgactgg
    14761 gctggtcaat gcattggaag tcgctagaga attccagctg aaatttttta cgccaagttc
    14821 aatcggcgcg tttggtccaa atactccaaa agatcatacc ccacaagata ctcttcagcg
    14881 tcctacaacc atgtatgggg taacgaaggt agctggagaa ctactatgtg attattatta
    14941 tgtgaaatat ggcgtagata ctcgtggtgt ccgtttccct ggattgattt cttatgcaac
    15001 cttgccagga ggcggtacga cagactacgc agtcgatatt tattattcag caatcaaaga
    15061 aggaaagtac actagtttca ttgatcaagg tacatcaatg gatatgatgt atatgccaga
    15121 tgcgatcgat gccattataa aactcatgaa tgcagatcct gatcgattgg ttcatcgaaa
    15181 tgccttcaat atatcggcga tgtcttttga cccggaagag atcaaagcat ctattcaaaa
    15241 aatcatacca gattttgaaa tggattatga tgtggatcct gtacgtcagt caatagcaga
    15301 atcttggcca aattcactag atgtttcttg tgcgcaagag gaatggggct tttcgccgaa
    15361 atacgactta gatgtgatga caaaggatat gttggaaaaa ctgaaagaaa aattggtaga
    15421 gacggtataa aacaagaacc acatccaaaa agttttttat acttttggga ttggctatgg
    15481 tatgaaaggg gacaagggat ggaagaatat caagccacgc cagtaaaaga agtaaaaata
    15541 gaaaatgaat taaaacgtac gatgggattt tttactgctt tatcgactgt tatgggcaca
    15601 gtgatcggtg caggggtatt ttttaaagcc gcaagcgtag cagaagttac aggatccgcc
    15661 agtttgcata tgttttcttg gtttctcggt ggtatgattt ctgtctgtgc aggactaaca
    15721 ggagctgaac tggcagctgc tattccagaa acaggaggga tgattaaata tatcgaacgg
    15781 atttacggaa atacagcagc ttttttattg ggctgggcgc aagtcgtgat ttattttcca
    15841 gcaaatgtag cagccttgtc gatcattttc ggtacacagt tcgtcaatct atttggtctt
    15901 tcccagtcaa tgattgttcc cgtagctgtt acagctgctg tatcgatcct gttgatcaat
    15961 tttttaggtt ctaaagctgg aggggctttt cagtcgatta cgcttgtctg caagcttatt
    16021 cctttgtttg tgatcgtcat tttcggtttg ttccgacaag aaggagtaga cttccaactg
    16081 tttccgatcc aagcaggaga aaatctttca tttttctcag ccttgggagc gggcttgcta
    16141 gcaacgatgt tcgcatacga cggttggata cacgtaggaa atatttcggg agaattgaaa
    16201 aagccggcga aagatttgcc aaaagcaatt tctttaggaa tcatcggaat catgatcgtt
    16261 tatttattag taaatgcggt gttcttaaga accgcatcta tcgacggagt cgcaggaaac
    16321 agcaatgcgg caagtgacgt tgcaaaaatg atttttggcg gcttcggtgg aagactggtc
    16381 acagttggta tcttgatttc tgtttatgga accatcaatg gctataccct tactggaatg
    16441 cgtctccctt atgttatggc aaaagaaaac aacctgcctt ttagcaagct ctttgctaaa
    16501 cttcatgata aaactaaagt tcctgtagcc gcaggtatct tagagctagt catcgctatt
    16561 ggcatgatga tgatcggtgg ttttgataca ctaacggata tgctgatctt tgtcatttgg
    16621 attttttata cgatggtctt tgttggtgtc attcttcttc gtaaaaaaga acctgatttg
    16681 tttcgaccat acaaagttcc aatgtatccg tttattccac tagtagcaat cattggaggg
    16741 acttttatcg tatcaagcac gcttatcact cagacctttt tagcttcgat ggggatagta
    16801 ttgacacttg ctggtatccc gatttatctc tatttaaaac gtagatagct aaaacattcg
    16861 aattttatta gaactgcact tctctgccaa acaaaatatt ttatttaaat gaggagtacg
    16921 tggaagaaat gtcaaaagta ctcgttaatt ttgatcacgt aaatgtcgtt ttgtctacga
    16981 tgaaatagca aagtaaaaga cagaaatcgg gctcaaccga tttctgtctt ttactttgat
    17041 ttgcgtaact gaactattcg gcgtacgccg cgataaatat ttaataaaac caagcaacca
    17101 gaaatgaaaa tgggaagcca aatcagatag acagggtagc caagcacttt agacaaagca
    17161 tgtggagtca ttttatccat ttcaagaagt gcttgctgcc aagtctgaag attgaagact
    17221 agcatgaaac ccgaaataat caggagaata aatcctaaat aatgaattgt agttcttttt
    17281 cttacataag cgatcgtagc ataaaggaac agtaaaaaaa taggtacgcc aaaaacaaag
    17341 tacatattca gcatctcgac accccctttg tgtttatctt actttacttt gaaagaaatg
    17401 agaaataata gcctcgttac ttgcttgaaa cagcaaagga ctgctaagat aagagagata
    17461 caaagaatca aggaggagtc atatggcaag ttatatcgaa ccgattcgtt ttgcgctgat
    17521 tgtttttcca tttttagcct tagcgatctc atctgttttt tttatttatg aatatcgaaa
    17581 gtatggaaca tttctagtaa caagggcagt tattttgtat tctttcgttt tttatttatt
    17641 gtgtgcgtat tttttggtga tcttaccact tccggcaaag gagacggttg cccaaatgac
    17701 agggccaaag atggagctgc atttatttgc ttcatggcaa catttcagag acaacacggt
    17761 attggtcttg tctgaaccta gtacctacct gccggcaatg aagcagagtg tatttttgga
    17821 accagctttt aatgttttat tactcatacc gtttggcatt tatttaagat attatttccg
    17881 gctttctttt atgaaaacga tgattgctag tttctgcctt tctctatttt ttgaattgac
    17941 gcagctaagt ggactttatt ttatctaccc aagaccatac cgcttatttg atgtgaatga
    18001 tttagtgaca aatacattgg gtggaatcat tggttttgtc gttacgccgt tatttacatt
    18061 tatgcttcct acaagaagac gaatggatga actttcttat gaaaaaggaa agactgtctc
    18121 attgactcga cgcttggtcg cttatttgat cgactggaca tttttatctc ttattaattt
    18181 ggtagcggcg attttcttta aaattgcgtt cgattcaggg gcagttactt ctcatatctg
    18241 gtggctttta ttagaagtag gggtatactt tatgattgta ccgtttataa ccaatggatg
    18301 gacacttggc aaaaaaattg tgcgtatccg tttggtggaa gaagaccgcg aacgaatttc
    18361 tttaaaagca ttgatgatcc gctacgggta tctgtacttt cttttttatg gtttaagctg
    18421 gttggggcag ttgcttcagc aaatcatgaa ttcgacaacg aatacggtgt tgcagatgag
    18481 tgcagtattc atttctctcg gtgtcttgtt actacaaggt atttttattt taaatatctt
    18541 gctatcattt ttgagaaaaa aacggattct gttttatgaa aaagctagtc acacatatac
    18601 tgttagcacg attggccaaa caaaggatga tgattctcga aattagctga gcaaaaacgc
    18661 tttgtctgtg ttataatgcc aaagtcgtga taatttaaag agaagaggtg cacgaatgaa
    18721 taattttgat atattccgct tcaaaccgtt tatcaatgaa gcgttaaccg caaagggctt
    18781 caaacagccc acagaggtcc aggaacgttt gattccttta attgcaaaag gaaaaaatat
    18841 cattgggcaa tctcaaactg gtacaggaaa aacacatacg tttttgttgc ctttgtttga
    18901 aaaaatcaat gcagaaaaag aggaagtaca agttgttatt acagctccaa gtagggaact
    18961 agctgcacag atctatgaag cagctacaca aatcgctaaa ttttctacac cggagatccg
    19021 tgtctcgaat tttgtcggcg gtacagacaa acagcgtcaa ttaaatcgtc tgcatcacca
    19081 acagccacag gttgtagtag gcacgcctgg tagaatatta gacatgatga atgaacaagc
    19141 gctgaagatt cacaccgcat ttgcatttgt tgtcgatgaa gcggatatga cattggatat
    19201 ggttttttta aatgaagtag accaaattgc aagtcgatta ccagaaaagc tgcagttttt
    19261 agttttttct gccactattc cagaaaaact tcgtccattc ttacgaaaat acatggaaaa
    19321 tccaatcatt gaagaaatca aaccaaaagc agttatttct gaagacattg aaaattggct
    19381 gatttcaaca aaaggtaaag atgagaatca aatgatttat caactgctga caattggtca
    19441 tccttattta gcaatcgtat ttgccaatac aaaacagcat gtggatgaaa ttgccgatta
    19501 cttgaaaaac caaggcttga aagtagcaaa gattcatgga gacatcaccc caagagagag
    19561 aaaacgagtg atgcgacaag tacagaatct ggagtatcaa tatgttgtag cgactgattt
    19621 agctgctaga gggatcgata tcgaaggtgt ctctcatgtt attaatgcag aaattccaga
    19681 agatctagac ttctttatcc atcgtgtcgg acgtactgga cgtaatggct taaaaggaac
    19741 ggccattacg ttatattcgc caaatgatga gcaagccatt gatgcgatag aaaaattggg
    19801 cgtctcattt gaaccaaaag aaatcaaaaa cggggaaatc gtcaaaacat acgatcgtaa
    19861 tagacgtgcc aaacgagaaa aatcaaaaga tgtattagat cccacgctga ttggtcttgt
    19921 gaagaagaaa aagaaaaaga tcaagcctgg ttataagaaa aaaatcgatt gggcaattgc
    19981 agaaaaaaat aaaaaagaac gcaaaatcga acgcagacag caaagccgta ctgctcgtaa
    20041 aaataagaaa aattcacata aataaaaaac aagaggttgt gacaaaaggc gtgtcacaat
    20101 ctcttgtttt taagctagac tttttttcat tccgatacaa agtacttcat cttcaataaa
    20161 aggttctgtg caagtatgat acccttgttt ttcataaaaa tctacagccg tcatttcagc
    20221 tactacataa ctagtctgac aaccttctga aaccgcccgt ttttcagcag cttcaagcaa
    20281 ctgaccgcct aacccttttc ctcggtattc ttttgctacg caaaatcgtt ccggcttgaa
    20341 gcaagtaggt gttttctttt cataacgcaa agtagctact ggtctttttt gatcaagaaa
    20401 tagataatag gtttgttgcg ggatatcttt tccgtcgaat tctagttctg caggaatccc
    20461 ttgttctaat acaaaaactt gatatctcaa aaagcagcta gctgcgcgta tccattcatc
    20521 cgctccaact atttttttca tcatattcct ccttgtcatg aatagcagag atgtattgat
    20581 tcctttattc ttatcgtacc aaaaatcgtg gagtatgacc aaaaggaaat gaaaataaaa
    20641 attgagatat tgacagtgat caatagaatg cgtatactaa ttaagtacga aaaggatatg
    20701 atttgttttt taagactcac agaaaattct ttcttggctg aaaaaagaat agtcactaat
    20761 caaattgctc cctttttcta ttttggtaga aataccgacg cagctcagcg ttatcgagtt
    20821 taagaggtaa tgattacaca tcattgcaat caaggtggta ccgcgagttt tcgtccttgg
    20881 ctgtgatggt gtttttttgt atttaattta tagaaagcag gtaatgacat gaaacaatta
    20941 agtagtgcac aagttcgaca aatgtttctt gatttcttcc aatctaaagg acatacgatt
    21001 gaacctagtg cgtctttgat ccccgtgaac gatccaacct tattgtggat caactctggt
    21061 gttgcaacat tgaaaaaata ttttgatggt tcagttgtgc cagaaaatcc aagaatcaca
    21121 aatgcccaaa aatctattcg aacaaatgat atcgaaaatg tcggtaaaac agcacggcac
    21181 catacgatgt ttgagatgct gggtaatttt tcgattggtg actatttcaa aaatgaagcc
    21241 atccactggg catgggaatt tttgactgat ccaaaatgga tggcattcga tccagaaaaa
    21301 ctttatgtaa cagtttatcc aaaagataca gaagcaaaac gcatctggca tgaagaagtt
    21361 ggcctgccac tagatcacat tattgatgta gaagacaact tctgggatat cggtgcaggc
    21421 ccatgtggtc cggatacgga aattttctat gaccgtggag aagcttataa cgatttagca
    21481 gaagacgatc ctgaaaacta tccaggagga gaaaatgaac gttatttaga aatctggaac
    21541 ttagtatttt cagaattcaa ccataagcct gataacacat ttgaaccact tcctcataaa
    21601 aacattgata cggggatggg attggaacgc atggtctcaa tcgttcaaga tgcgtcaacg
    21661 aactttgaga cagacttgtt tatgcctatt atccacgccg tagaaaagct aagcaaacaa
    21721 gtaaaatatg gtgaaaaccc agtaacagat atttcttaca aagtaatcgc agatcatatt
    21781 cgcgcccttt catttgctat tggcgacggt gcattgccat cgaatgaagg gagagggtat
    21841 gtcttacgcc gtttgcttcg tcgtgctgtc atgcatggga aaaaattagg tattgaacaa
    21901 gcattcttat atcgtttagt ccctgttgtt ggggaaatca tggtaagtta ttatcctgaa
    21961 gtacttcaac aaaaagaatt tattgaaaaa gttatccgta cagaagaaga acgtttcaat
    22021 gaaacaatca atgaaggttt ggaaatctta aatcaagtga tccaagaagt aaaaaacgaa
    22081 aacgggacga ctctagcagg aaaagacatc ttcaaactat acgacacata tggcttcccg
    22141 gtagaattaa cggaagaggt ggcagaagat gaaggactga aagttgatca tgctggtttt
    22201 gaaaaagaaa tggatgcaca acgcgaacgt gctcgctctg cccgcagtac tgaaaaatca
    22261 atgggggtac aatctgcttt attgacagat atcaaagtag aaagtaaatt cattggatac
    22321 acagaacaag aatcagatag tcaattattg gtcatcgtta aaggtgagtc tcttttgaat
    22381 gaagtcgcag aaggtgaagc tcaaatgatc tttgatcaaa cacctttcta tgcagaaatg
    22441 ggtgggcaag tagcggatca aggatggatc atgaatgaag taggcgaaat cgtagcagtt
    22501 gttacagatg ttcaaaaagc gcctaatggt caatttatgc acaccgtaga agtaaaagca
    22561 ccaatgagtg aaggacaaac ctatcatcta ttgatcgatc aatcgatgcg caatcggatc
    22621 atcaaaaacc atacagcaac ccatctactg cacaaggcat tgaaagaagt acttggcgag
    22681 catgcaaatc aagctggttc tttagtcgct cctggacatt tacgattcga ctttacccac
    22741 tttggtcaaa tcacttcaga tgaattagct gaaatggaaa gaatcgtcaa cgaaaaaatc
    22801 tgggaagcta tcccagttga aacaattgaa acagatatcg atacagccaa aaacatgggt
    22861 gcaatggctt tgtttggtga aaaatacgga catgacgttc gagtagtaaa tattggcggc
    22921 tggtcaatcg aactgtgcgg aggcacacat gtagcaaata cagaaaacat cggaatcttc
    22981 aaaatcgttt ctgaatcagg aattggtgca ggcgtgcgac gaatcgaggc tgtaacaagt
    23041 aaagaagcgt acgaactact tgaagaagaa gaaaaacaac taaaacaagt agcaacaatt
    23101 gttaaatcac ctcaattgaa agaagttgtt tcaaaagcgg aacaattgca acagcaagta
    23161 agagagctac aaaaggaaaa tgaacaatta gctagcaagt tagcgaatca acaagctggt
    23221 gatgtattca aagatccaga aacgatcaat ggaaaaacaa tcattgcagc acaagtaaaa
    23281 gtgaaagata tgaatcagct gcgtcaatta gctgaccaat ggaaacaaaa aaatatttca
    23341 gatattcttg ttttagcaaa cgaacaagaa ggaaaagtca gtctgctagc ggcaatgacg
    23401 aaagaatcaa atgaagctgg actaaaagct ggcgacttga ttaaagcaat cgcaccgaaa
    23461 gtaggcggtg gcggtggtgg acgtccagac atggcacaag ctggcgggaa aaatcctgct
    23521 ggtattccag atgcacttaa agctgcgaaa gaatgggttg cgcaacaata gggcgtttac
    23581 gtttgacaga aaaacaaaag ttcaaacatt gaacgaaaca tgattatggg ggcgaagaca
    23641 aacaagtctt gccctcatac ttttttcaat tggatggcgc atctaatcaa atggtcggat
    23701 tcggatggtc tttagttgac gaaagtgaca gaaattttta cgattagtat aaataaaaag
    23761 gggtgaaaaa atgaatagta gaaataagac ctttcgacta gtcttacgtg cgatcttgtt
    23821 agcaatcatc atcgtacaag cgatggttcc ttggctgggg tttattccat taggattcat
    23881 tagtttaaca attatccata tcacagtaat tattgctgca gtagtattag gcccaaaaga
    23941 tggcatggtg attggcttgt tttggggaat cgctacaatt gttcgtgcct atgccatgcc
    24001 aacaacgcca tttgatacat tagtgttcac gaatccgatt atctcggtag ttcccagagt
    24061 acttgttggc ttagtagcag gcttggtttt ccactggatt tatcaaaaaa ataaatcaat
    24121 aacgatcagc tcagcctttg caggggtact gggttcattg gtcaatacag tcttagtgtt
    24181 aggattcatg ggattatttt atacaggtgc aacggcagat gcctatggtg tagactctgc
    24241 tcttttattt aaaaccttag caggggttgc agcaatcaat gggataccag aagcaatagg
    24301 tgcgggaatc ataacaccat tactagcaaa agcgttattt gcagcaacac cgttgaaacc
    24361 agaataatga ccatctttta atagtaatag gacgcacgat agatagaaaa aggaagagag
    24421 gagaggaaaa caatcgtcct ctccatcttt ttacctgagc aacaagcaaa ctagaagggg
    24481 taaaaaactt ggctaagaaa caagaagaaa aaaagaaaca aataccgcaa cagcggacaa
    24541 aaaaaggagt actagcgggt atcttaggat tggttacgaa tctgatcttg tttgtctcta
    24601 agctaatgat cgggcttttt tcaggaagtg tctccattat ggctgatgca gtgaacagtc
    24661 tatcagatac tgcttcatcc atattgactt tgatcggatt taaaattgct gcaaagccag
    24721 cagatcagga acatccttat ggacacgaac gcttcgaata tatcagtggg ttatttgttt
    24781 cgatcatcat tacttatgta ggatttcaat ttttagagtc ttctgctgaa aaaatcatcc
    24841 atccagaaaa tgtctcttta acacccatcg tcttttttgt cctaatcatt tccatctttt
    24901 tgaaatttgc acaaggcaaa atgtatcagg ttattgcgca atccattgat tctgaaacat
    24961 tgagagctac agcaacagat agttataatg atgtctttac tacactggct gtattgcttt
    25021 cagcagcaat cgaatgggta actggatggc gtattgatgg atatataggt tttgctttag
    25081 cattatatat cctttatagt ggattgacga tgcttcgaaa ttttgtatac gaactattag
    25141 gaagtcgccc aacgccagca gagattgaag aaatggaaga gcatttaaat gcttgcgatg
    25201 atatacttgg ctatcatgat ttattggtgc ataactatgg acccaataaa cgctttgcat
    25261 cagtccacat cgaagtagat gaatcaatga atttgaatgt ggctcataag attattgatc
    25321 tgatagaaaa agactttaaa aagtctttgg atgtggaact tgtctgtcac ttggatccaa
    25381 tgcctgttga taacgaaaaa tactataata tcttgcggca gttaaagcaa attcttcttg
    25441 aaacaggtac agggcttaat attcatgatt ttagagtaat tcatcaagga aaaacgctgc
    25501 aatttgatgt agtggtccct gagaaattcc agtttgttga tacagaacta gaaagaatct
    25561 tacgtagtca aatagaagaa aaaatcggag attaccaatt ggaaattacg tttgatcaca
    25621 attatctttt atattaaatg agaaactata aaaagactcc ttaaagtttt taatttaaag
    25681 agtctttttt tcttttaatt gtttatggag agcctgacgt gcaagattat ctgctccttt
    25741 atttttgttt tcgggcaacc attgcaggat caataaagaa aattcggata gaagttcttg
    25801 gattctttga aggtagggaa ggaagagctc gttcttcgtt gcgtttttgt cgaccgtaga
    25861 aactagtgtt ttgctgtctg aaaaacagaa aatcgtttca ttctgtaagt cgtttttctt
    25921 tagaaattct aaagttttta aaaagacttc gaactctgct tgatggtttg tccctataga
    25981 aagcgggata gatagctgtt cttgtttacc ttgatagaca atcaagattc cacctccact
    26041 aggaccaggg ttgccttttg tagaggcatc aatgtatact tttaacataa aaagagattc
    26101 tcctttttgt tcaaactaat gttatggtaa gattatatca ttactgaatc ggtgagaggt
    26161 tagagaatga aaaaaaatat tcgatggcag cctgaactgg cacatacgat catctattgg
    26221 tcattgacct tgatcattct gttttttagt ctgattcttt ctttagaaaa cacaggtcct
    26281 tattggaaaa gcaattttgt tttaggtatt tttcttgttt ttctatttct aggtttgcgg
    26341 cgttctttta ttttaaaatc aaactctgtg attatacgtt atgctgcttt ttggaaagac
    26401 cgagaaatat ctgtcggaca gattcgatct ttgacgccaa ctaatactgg ggtgcaaatg
    26461 atgttgcaag atgagaaagc cccatctgtt ttcatgatgc gaaaaaaaga tcaagtagtc
    26521 ctttttgatc attttgaaaa aaacggaccc aaatcagaat caattcctta tccttatacg
    26581 aaaaaaagaa aaagctaatt ttaaaaatca gaacctaaaa aaacgaattt ttacaggaaa
    26641 agaaaagagt gagacgtacg gttcatcccg tttagcgtct cactctattt gtttatctat
    26701 tcatgcatac tagtatcggt agtctgaacg ttgcctttct tgacatgggc agcttgtgtt
    26761 cctcgattcc cttcgatgat ctcaaattcg accacttgat tttcttccaa agttttgaat
    26821 ccctcttctt caatcgcagt aaaatgtaca aaaatttctt ctgtgtcatc ataactgata
    26881 aagccgtatc cttttttgtt gtcaaaccat tttacaattc ctgtggtcaa agcactcacg
    26941 acctttctaa agtctgatca tagccttcat tataaagagt atttataagt acgtcaaata
    27001 aattaaaaac ctgtaatttt atgttttttt tctgaataaa aaaaggtata attggcatat
    27061 cttaacaaag aagatatttt gttgaataaa gttcgtgaaa aggggtaatc aaatgaagaa
    27121 tgatctgcaa atttcacaag aaacagagct agaaccgatt gttgaagtgg ctagtaaatt
    27181 agatcttaag gaagacgatt tggaactgta tgggaaatat aaggcgaaaa tcaatttttc
    27241 tggaatcaat cgatcaaagg ataacgctga ggggcatttg atcttagtta cttccatcaa
    27301 tccaacacca gctggcgaag gaaagtcaac gatcactgta gggttaggag acgcgctcaa
    27361 taaaatcaat aaaaaatctg ttattgcatt gcgagaaccc tcacttggtc ctgtgatggg
    27421 aatcaaaggt ggtgctgcag gcggaggata tgcacaggta ttgcctatgg aagatatcaa
    27481 cttgcatttt actggtgaca tgcatgctat cacaacagct aataatgcat tatctgctct
    27541 tttagataat catattcatc aaggaaatga attgaacatc gatgcccgcc gagtgatttg
    27601 gaaacgggta gtcgatctca atgatcgtga actgcgtaaa gtaattgttg gcttgggtgg
    27661 gccaatccaa ggtgttccaa gagaagatgg gtttgatatc acagttgcca gcgaaatcat
    27721 ggctatcttg tgtcttgcgg cagaccttga agatttaaag gctcgtcttg caaggatcgt
    27781 tgtcggctat acatttgacc gtcagcctgt gactgctgga gacttgaaag cagaaggcgc
    27841 tctggcatta ctgctgaaag atgcaatcaa gccgaatctt gtacaaacaa tttacggaac
    27901 accagctttt gtccacgggg gaccgtttgc caatatcgct catggctgca atagtatttt
    27961 agctaccaag acagcattga gattagcaga ttacgttgtc acagaggcag gatttggtgc
    28021 agatcttggc ggagagaaat ttttagatat caaagtacct aacttgaaaa aagcgccgga
    28081 tgctgtggtg attgtcgcca ctgtccgtgc attgaaaatg catggcggaa tgaaaaaaga
    28141 tgaacttaaa aatgagaatc ttgatgcttt gaaagtaggt tttgcaaatt taaagcgcca
    28201 tatacgtaat atggaacaat atcagctacc tgtgatcgtc gcaatcaatg aattcgtaac
    28261 agatacagac aatgaactca cactgttgga gcacttgtgt gaaaatcagg ggattcttgc
    28321 caagagagcg agtgtatggg cagatggagc agaaggcggc gttgatttag caaaagctgt
    28381 agtacgattg attgatagaa aagaagctaa ttacaagcca ttataccgcc tagaagaaac
    28441 gatacaagaa aagacagaaa caatcgtcaa gaaaatttac ggaggaaatg gtgttgtttt
    28501 cagcaaaaaa gcgcaaaaac aaatagaaga gtttacgaag aatggatggg acaaactgcc
    28561 gatttgtatg gcaaaaacac agtattcatt ttctgatgat ccttcactgc taggtgcacc
    28621 ggaagatttc accatcacga tccgcgaaat catcccaaaa ttaggggcag gatttttagt
    28681 agccttgact ggagatgtta tgacgatgcc tggtttgcct aaaaaacctg ctgcattgaa
    28741 tatggacgtg acaaatgacg gagaagtgtt gggattgttt taagcttgat tataatctac
    28801 catcgaagga gctgtatttt gactacagct tcttttttta gacgataggg aattaatttt
    28861 gtttacacta ggatcattct ttttctttat tgtattttga gctatactaa agcataatag
    28921 gagggtatga tgaaaacgac atttataaga aaaaccggtt tttacggtat gggtagctcg
    28981 attcagctaa gaatcaacaa tgaaaaacgc agtgttctca gtcatgaaca aaagataacg
    29041 cttgatctgg aagtgccctt tacgatgcag gtcacctttt attggctaaa aagccccgtt
    29101 tatacagtga aagaagcaaa gcctgcttat gtgatcacaa tgaattactt gattcttcaa
    29161 atctatccgt ttttatttct agcaactggg ttgggggccg ttttcctaca aaatttattt
    29221 ttcagttttt ttgcagttat tgtgttgatt gcgttctttt tctatataaa aaaccgggca
    29281 tatttgatca aggaggaaaa ttatggcaaa ctctgatctt tttttaatca gtttcaatcg
    29341 aatcgaaaaa tggatgagag acgagatggg aaatgcacga aatatgggat ttaccgaact
    29401 tgtcagacgc ctagctcaaa gaaaacaatt aatgatcaga aaatacgaag atgatctttt
    29461 acagcttgct cagttaagga atgcgatcgt ccatgatcgg atcgcagtcg attttgtcat
    29521 tgcagagcca aatgattgga cagtcaagcg aatccagctg atcgaacaag aactgattca
    29581 gcctgaaacc gtgctgcctc gatttgcaaa acgtgtaaca ggttttgaac aagatcttcc
    29641 gttacttgaa ctgttaaaga tagtagcaga aaaaaggtat tcacaatttc cgttatacaa
    29701 taaaggaaaa tttgtagcat tgattacttt acgtaatatc gggttttggc tagcaaaaga
    29761 aagccaaaaa ggtccggttg atttaacaaa taaacgagcg ttggatttga tcatccaaaa
    29821 cggaaagtac acaaattatc attttgttcc agctaaaaca catatttacg aggttgaagc
    29881 gatgtttcgt gagcaaggga ctttggaagc cgtacttatc actaaagatg ggaatcccga
    29941 tggtaatctg ctcgggatcg tacgacctcg tgatatttat aaacaaatag aaaaggattg
    30001 attttttgtt aattatttat tggatattca gcgctttgct agtaggagtg gatcagttgg
    30061 tcaaatggtg gatcactgat aatctagcgc ttggagagac gaaaaatctt ataccaggta
    30121 ttttgtcact gaatcatatt cgaaacactg gagccgcatg gagtatgcta gaagggaaga
    30181 tgtggttctt tactatagtg acactcattg cagtcgttgt gattctcaca ttgatgatta
    30241 aaaatcgtga aaaaggtaat cgctggttca tgattggatt gagtttgatt ctagctggtg
    30301 cgattggcaa ttttatcgac cgtattcgtc ttggctatgt agtagatatg ttccagacag
    30361 attttatgag ctttccgatt tttaacgtag cagatgtgac acttgtatgt ggagtgattt
    30421 gtatgttaat ttatattatt ttagatgaaa aggatcaaag aaaaaaatga cagaacttaa
    30481 aacgacgatt caccatgaat ctggtaggat cgataaagta ttgactaatt tattcaccga
    30541 ttacagtcgt tcgcaaattc agctgtggct gaaaaatgga gcggtttctg tcaatggaca
    30601 gccagtcaaa gcaaattaca aagtaaaaca aggagacgag attactgtct tgatccccga
    30661 accagaaaca ttagatgtgg tcccagaaaa tatcccgttg acgatcgttt acgaagatga
    30721 agatgtagcg gtgataaaca aacctcaagg aatggtcgtt catccttcag caggtcatac
    30781 gcatggcaca atggtcaatg cgctgatgta ccatatcaag gatctttcta cgatcaacgg
    30841 cgttattcgt ccaggtattg ttcaccgaat cgacaaagat acttccggac tgctaatggt
    30901 tgccaaaaat gaccaggcac acgaatcatt agctcagcaa ttaaaagata agacctcttt
    30961 gcgcaaatat gttgctttag tccatggaga gattcctcac gaaaaaggaa ccatcaatgc
    31021 gccaatcggc cgttcgaagg aaaaccggaa aatgcaggcg ataagagaag atgggaagtc
    31081 ggctgtcaca cattttactg tattggaacg tttcgaagga tttactctgg ttgaattaaa
    31141 actagaaacc ggaagaacac accagatccg tgtgcatatg aagtatatcg gttatccgtt
    31201 agctggagat ccagtatacg gaccgaagaa aactttgaaa ggcaatggtc agtttctcca
    31261 tgcgcaaatg ttaggtttta cgcacccgac tacaaacgaa aagatgatct ttgaagcacc
    31321 tcttccagaa gtttttgaaa aaactttaga aaaattaaga aaaaacattg cattttaatt
    31381 tagataacgg tatagtaatt aacaacgaaa gcataaaact taatagaatt cctttaagtt
    31441 tagtcccgtg aggctaagaa gggtgggagc aaggatacgt atggtgcgcc ataagtagat
    31501 tgctcattga catagtatag ctatcagtgt atcagtatac gctcagtcta aacattcctc
    31561 ctgtcctctt agggcaggag tttttttatg caaaaaagta gggggaatga aaatgcaagc
    31621 aaaagaagta gtagatcagg taaccatgaa gcgagcattg actcgaatta cttatgagat
    31681 tatcgagaga aaccatagta tccaagatat cgtattggta ggtatcaaaa cacgaggaat
    31741 ctacattgca tcaagaattg ccgaacgcct gaagcagcta gaagatatcg atattcctgt
    31801 aggagaactg gatatcactt tataccgaga tgacaaaaaa gaaaatccag aagaaccgga
    31861 acttcattct tctgacatcc cagtttcctt agaaggaaaa gaagttattt tgattgatga
    31921 tgttttgtat acaggacgaa cgattcgagc agcaatggac gcagtaatgg attttggcag
    31981 accaagaaaa atttcattag cagtcctggt cgatcgtggg catcgagaat tgccaatccg
    32041 tgcagattat gttgggaaga acattccaac atctcgggcg gaagagattt tagtagagat
    32101 gcaagagctt gatggacaag atcgcatcat gattttgaaa gaggaagatt agaggagaga
    32161 gaaacatgtc ggagaaaaaa ggatttcata atgatgacgt ggtgttagat atccacgatc
    32221 gcccaacccc aggacattgg gtaggattaa gtttacaaca cttgttcacg atgttcggag
    32281 caaccgtgtt agtccccatc ttagtaggta tcgatccagg tattgcgcta gtcagttcag
    32341 gactaggtac attggtgtac ttatttacta ctaaaggaaa gatacctgcg tacttaggaa
    32401 gcagcttcgc tttcattgca gcgatgcagc ttttgatgaa aacagatggt tatccagcga
    32461 ttgcacaagg agcagtcaca actggattag tctatttgat tgttgcgctg atcatcaaaa
    32521 agatcggttc agcatggctg gataaaatct tgcctccaat cgtcgtcggt ccagtcatca
    32581 tggttatcgg gttgggactt gcggcaaatg cagcaacaaa tgctatgtat aatcagggca
    32641 cgtatgattt caagtatatt gctgtagcgc tgatcacatt aggattaaca attttcttca
    32701 acatgtggct aagaggtttt ctcggattga ttccgatttt actaggtatt attagcggat
    32761 acatcgtagc cttgctgttt ggaatcgtgg atactaagcc aatcgtagac gctgcctggt
    32821 ttgctgttcc taactttgag attccctttg tacaatatga accaaagcta tatcttggag
    32881 caatcacaac aatggcaccc atcgcttttg tgacgatgac agagcatatc ggccatctta
    32941 tggtattgaa taaactaact aaacgtaatt tctttgaaga accgggtctt cataagacgt
    33001 tgacaggtga tggattagca caaatcgtgg cagcctttgt cggaggtcct cctgtaacaa
    33061 gttacggtga aaacattggt gttctggcta tcacacgtgt ccatagcgtg tttgtgatcg
    33121 gtggagctgc agtatttgca gtcattttag gattcgtagg aaaattaagc gctttgattt
    33181 taagtatccc tggaccagtc atctcaggaa tcagttttat cttatttgga gtaattgccg
    33241 caagtggaat gaagatttta atcgagaaca aaatcgattt tgataaaaag aaaaatctgt
    33301 tgattgcttc agttatctta gtagtaggaa ttggcggact cgtctttgaa gtaggcacat
    33361 ttacattgtc ggcaatggca ttagcaactg tacttggcat cattttaaat cttatcttac
    33421 cagaaacttc acgaagcgaa gaaaactaag gaggaaacat tatgatcatc aaatcagaac
    33481 gtatcagctt aaagcattta ttgaccgttg aggcacttac tgatcaagaa gttatgggct
    33541 tgatccgcag agggcaggaa ttcaagcaag gtgctaattg gactccgcaa aagcgccagt
    33601 actttgctac aaacctcttt ttcgaaaata gtactcgcac acataaaagc ttcgatgtag
    33661 cggagaaaaa actagggcta gaagtgattg aatttgaagc aagcacaagc tctgtccaaa
    33721 aaggggaaac actatacgat acagtactga cgatgtcagc attaggcgta gacgtagcgg
    33781 ttatccgtca tggtgatgag aactattatg acgaactgat acaaagtaaa acaatccagt
    33841 gttcgattat caatggagga gacggaagcg gacagcatcc aacccaatgc ttgcttgatt
    33901 tgatgaccat ttatgaagaa tttggtcatt ttgaaggtct gaaagttgcc atcgtaggtg
    33961 atatcactca ctctcgcgta gctaaatcca atatgcagat gttaaaacgt ctaggcgcac
    34021 aagtattctt ctcaggtcct agagaatggt acgacgatga attcgaagtg tacggacatt
    34081 atatgccatt ggatgaacta ctagatcaag ttgatgtcat gatgatgctt cgggtgcaac
    34141 acgaacgcca tgatggaaaa gaaagtttct caaaagaagg ctaccatcag gaacatgggt
    34201 taacagttga gcgtgcgaag aagttgcaaa aacatgcaat cattatgcac ccagcaccag
    34261 ttaaccgtga tgtagaatta gctgattcat tagtggaagg attacagtcg cggatcgttc
    34321 agcaaatgag caatggagta ttcgtcagaa tggcaatctt agaagcagtg ctaaatggaa
    34381 aagcgtaatt ataggaggat gaaaaaatga aaacactcat taaaaacggt cagatcaata
    34441 cacgaaaaaa tatgacgaca ccagcagaaa tttggataga agatgggcga atcaaagcaa
    34501 tcggtacagg attctcagaa gccgaatttg atgaagtgtt tgatgcaaag ggacaattga
    34561 tcacacctgg tttagtggac gtccatgttc atttgagaga gccaggattt acatacaagg
    34621 agacgatcga agctgggact cgctcagctg ctcgtggcgg tttcactact gtatgcgcga
    34681 tgccgaattt aaatcctgta cctgatacgg cagaaaagct gagacaagta tatgacatca
    34741 ttagaaaaga tgcagttgtg aaggtattgc agtatgcgcc aatcacagaa aatctgcgaa
    34801 gtgagaaatt agttgatcaa gaagcactga ttgaagaagg tgcttttgct ttcacaaatg
    34861 acggtgtagg tgtccaaaca gctgggacga tgtatctggc tatgaaagaa gctgcaaaaa
    34921 acaataaagc cctagtagcc catacagaag atgaatcttt attatttggc ggcgttatgc
    34981 acgctggcaa gaaggcagaa gaacttggtc ttcccgggat cttaagcgta accgagtctt
    35041 ctcaaattgc tagagatctg ttgctagctg aagcaactgg tgttcattac catgtttgcc
    35101 atgtttctac taaagaaagt gtccgtgtaa tcagagacgc aaaaaaagca ggaatccacg
    35161 taacagcaga agtttcaccc catcatctta ttttgatcga cgaagatatt cccgaagatt
    35221 tcggcttttg gaaaatgaac ccgccgctaa gaggacggga ggacagagaa gctttgatcg
    35281 aaggcttgct tgacggtacg atcgattgca tcgctacaga ccatgccccc catggattag
    35341 aagaaaaaag ccaaagtttc atgaaatcac catttggtat cgtaggaagc gaaacagcgt
    35401 ttcaactgat ctatactcat tttgtagaaa ctggtcgatt cacgttggaa caagtgatca
    35461 attggttggc agttaaaccg gctgagattt ttggtttgaa tgcaggaacg ctaacggttg
    35521 gtgcccccgc tgatgtagca gtttttgata ttacacaaac ttgcacgatc gataaagaag
    35581 actttttatc aaaaggggag aatacacctt ttatcggctg gaaagtaaaa ggcgaaacac
    35641 aaatgacttt tgtcaacgga aaacttgtgt ggcagaaggg agaataaaag tatggaacgt
    35701 ttgcttatat tagaagatgg aacagttttc gaaggaaagg cttttggtgc agatgctagt
    35761 gtcttcggag aaatcgtttt tacaacaagt atgactggtt atcaagaaac tatcactgat
    35821 caaagtttta acggtcaaat cattacattt acctatccta tggtgggtaa ttatggggta
    35881 aatagagatg attatgaatc gatttctcca acatgcaaag gagtagtagt aaaagaacat
    35941 gcacgtgtag cttcaaactg gcgaaaccaa atgacgctag attcattttt gaaacaaaaa
    36001 ggaattcctg gaatatcagg tatcgatacg cgcgccctta ctcgcaaaat ccgtgaagtc
    36061 ggaacgatga aagctagtat agttgatgct ggagattcct tcgaacatgc atttgatcag
    36121 ctgaaagcaa ccgttctagc aaccaatcaa gtgcagcaag tctcaactaa caaaccttat
    36181 ccaagtccag gaacaggtcg gaaagtcatt gtcattgact tcggcttgaa acatagtatt
    36241 ttacgagaat tgagtaaaag aaactgtaac ctcacagtac ttccctataa cacagatgcc
    36301 gagacgattt tagcgctatc accagacgga gtcatgctga cgaatggacc tggtgatcca
    36361 aaagatgtgc cagaagcaat cgaaatgatc aaaaaaatcc aaggaaaagt accgattttt
    36421 ggtatttgtc tcggtcacca gttatttgct ttagccaacg gtgcagatac ctataaaatg
    36481 aaattcggac atcgaggatt aaatcacccg gtacgagaag tagcgaccgg aagaattgat
    36541 tttacttctc aaaatcatgg ctatgcagtc gatgaagcca cgattgatcc agaaaaactc
    36601 atcgtaactc atgtagaagt aaatgacgga acagtagaag gagtcaaaca cagagattat
    36661 cccgctttca gtgtgcagtt tcatccagat gctgcccctg gtccgcatga tgcactacat
    36721 ttatttgatg aatttatgga aatgatggac gcaggaaagg agcagtaaac agatgccgaa
    36781 acgtacagat atcaagaaaa ttatggtgat tggatctggt ccgatcatca ttggtcaagc
    36841 cgcagaattt gactacgcag gaacacaagc ctgccttgcc ttaaaagaag aaggatatga
    36901 agtggtttta gtaaattcta atcctgcgac gatcatgacg gataaagaag tggcagatca
    36961 agtatatatc gaaccgatca cacttgaatt tgtatcaagg attcttcgaa aagagcgtcc
    37021 agatgctctc cttccaactt taggcggaca gacaggcctc aatatggcaa tggagctggc
    37081 agcatcaggt attttagaag aattgaacat agaacttcta ggaacaaagc tttctgcgat
    37141 cgatcaagca gaagatcgtg atctattcaa acagttgatg aaagaattga atcagccaat
    37201 ccccgagtct gaaatcgtca atacagtaga agaagctgta gattttgcaa ataaaatcgg
    37261 ctatcctgtc atcgttcgtc cagcctttac tttaggcgga actggtggag gtatgtgtga
    37321 aaatgaagaa gaattacgta tcgtatcaga aaatggatta agtctttccc ccgctaccca
    37381 atgtttgatt gaacgaagta ttgcaggttt taaagaaatt gaatatgaag tgatgagaga
    37441 ttcggctgat aatgcaatcg ttgtctgcaa tatggaaaat tttgacccgg tgggtatcca
    37501 tacaggtgat tctatcgtat ttgcccctag tcaaacattg tctgaccatg aatatcaaat
    37561 gcttcgtgat gcttctcttt cgattattcg agcacttaag atcgagggag gctgtaatgt
    37621 ccaactcgct cttgatccac atagttttaa ttattatgtg attgaagtca atccgcgtgt
    37681 ttctcgttca tctgcgttag caagtaaggc gactggctat ccgatcgcta agctagctgc
    37741 taaaatagcg cttggattaa cattagacga aatgaaaaat cctgttacag gaacgacttt
    37801 tgcagaattt gaacctgcat tagattatgt tgtagctaaa attcctcgct ggccgtttga
    37861 taagtttgaa aacggagaac ggcttttagg cacgcagatg aaagcaactg gtgaagtgat
    37921 ggctatcggt cggaatatcg aagagtcttt attgaaagct gtgcgttctt tagaaatcgg
    37981 tacacaccat atcgaacttc ctgagttggc agacatcacg gacgatgacc taatccaaaa
    38041 agtggttcga gcacaagatg atcgcctgtt ttatgtagct gaagctatta gaagagggta
    38101 caccatagaa gaattagcgg atttgacaaa aatcgattta ttctttttag ataaactgtt
    38161 gcatatcgta gaaatcgaac agcagttgaa acagcagccg ttggatgaca agatattgaa
    38221 aacagcgaaa caaaacggtt ttacagacaa aaaagtggcg gagctgtggg aaataaacga
    38281 agaagccgtt cgccagatga gaaaagaaaa tgatttgatt cctgtatata agatggttga
    38341 cacttgtgca gcggaattcg aatcaaaaac accttatttt tatagttcct atgaatatga
    38401 aaatgaaagt tcacgatcag aaaagccctc aattctagta ctgggatctg gtcctatccg
    38461 tatcggacaa ggggttgaat ttgactatgc gactgtgcat tctgtcaaag caatccagca
    38521 ggcaggatac gaagcaatca tcatgaacag caatcctgaa acagtttcga ctgacttttc
    38581 tatttcggat aaattatatt ttgagccatt gaccttcgaa gatgtgatga atgtgatcga
    38641 gctagaacag ccgataggag taatcgttca atttggtggt cagacagcaa tcaacttagc
    38701 cgaacctttg actgctgctg gtgtaaaaat cttagggaca acgatcgaag acttggatcg
    38761 tgcggaaaat cgtgatttat tcgagcaagc gctgaaagag ttggatattc ctcaaccttt
    38821 aggggatact gctacaagta aagaagaagc cgtggttatc gcttcgagaa ttggttatcc
    38881 agttcttgtt cgtccaagct atgtattagg cggacgtgcg atggaaatcg ttgaaaacca
    38941 gcgagattta gaagattaca tggaacatgc tgtgaaagca tctccagaac atccagtact
    39001 agtggaccgt tacttgcttg gtggtgaatg cgaagtggat gcgatttgtg acggtgaaac
    39061 agtactcatc ccagggatca tggagcatat tgagcgtact ggtgtccatt caggggattc
    39121 aatggctgtg tatccacctc aaacattatc tgaatcgatc aagaaaacga tcgaagatta
    39181 cacgatccgc ttagcattag gtttgaattg tgtcgggatg atgaatatcc agtttgtcat
    39241 ccatgaaaat gaggtttatg tgatcgaagt caatcctcga gcaagtcgga ctgttccatt
    39301 cttaagtaag gtgacaggaa ttccaatggc acaaattgcg acgaaagcta ttttgggaga
    39361 gaaactaaag acacttggtt atgaaaacgg actttatcca gaaacaaaag aagttcatgt
    39421 taaagcacct gtcttttctt ttacgaaatt gcagaaagtt gatacttact tagggcctga
    39481 aatgaaatcg actggagaag taatgggatc tgacagacaa ttagacaaag ctttgtacaa
    39541 ggcattcgaa gcttctggat tacacttgcc tgaatatgga gccgtattat ttacgattgc
    39601 ggacgaaaca aaagaagaag cactagaatt agctaaacgt tttacagaaa ttggctatag
    39661 tctgatagga acgaaacata cagctgctta tttcgaaaaa aatggtctgg tcgttgaaac
    39721 agtagcgaaa atttctgagg aagcagcaga aaaaaatgta gtcgacttaa taagagatgg
    39781 taaaacacaa gtggttgtca atacgattga taaagatcgg ggaaatgctt ctaaagacgg
    39841 tttcatcatc cgaagagaag cagttgaaca tggtattcca ttgtttactt ctttagacac
    39901 agcagatgcg atcgttcgag tgatggaatc ccgtgctttt tcaactaacc ctatttaacg
    39961 gcgagcacat gttaagacaa aaagacgaaa aaccgttgca ggaggggaca gatgaaacaa
    40021 gaaatgatgg aaatcgttca gcaaaaacag ctggcaccaa agatttttga aatgacattg
    40081 aaaggtgatc tagtaaaaga aatgcagcaa ccaggacagt tcttgcatat tctcgtgccg
    40141 cgtgcagact tgctattacg ccgaccgatt agcttgaatc atatcgacca cgaaacaaat
    40201 acttgccgta tcatttatcg ggtggaaggt gaggggacta gagtcttctc tactctttct
    40261 gccggagacc atttagatgt catgggtcct ttaggcaatg gttttgattt gtccaatctt
    40321 catacaggag acgaagtatt tataatcggt ggaggaatcg gtgtccctcc tctatatgag
    40381 ttatcaaaac agttagtcga aaaaggaatt aagccaacgc attttttagg ttttgccaca
    40441 catgaagtcg tttattatga aaaagaattt aatcgtctag gagaaacaag aatcgctact
    40501 gacgatggaa catacggtat gcacggaaat gtcggtaatt tattgttagg aacgaagacg
    40561 gaacctacag ctgtttttgc atgtggttca aacgggttat tgaagacagt ggaacagctg
    40621 ttttcttcgc atcaaaatgt ccagtcatca ttagaatccc gcatggcatg tgggataggg
    40681 gcttgctatg cttgcgtttg tcatacgccg gaagatgaaa caggaacaaa aagtgtgaaa
    40741 gtttgtgacg aaggaccgat ttttaaaata ggagaggtaa ttatatgaat gagaatcctt
    40801 tagcggtcaa acttcctggc ctagaattaa aaaatcctat catgccggca agcggctgtt
    40861 tcggttttgg caaagaatac ggaaaatact atgatttgaa tcagcttgga tcgatcatgg
    40921 tcaaggcaac gactccaaat gcccgctttg gcaatcccac gccaagagtg gcggaaacac
    40981 catctggcat gttgaatgca attggtttgc aaaatcctgg tctagacgtt gttatgcata
    41041 aacttcttcc agaattagag gagtacaatg agttacccat catcgcaaat gttgctggtg
    41101 cttgtgaaga agactatgta gaagtttgta gcaaaattgg cgaagcacca aatgtgaaag
    41161 caatcgagct gaacatttct tgcccaaatg tcaaacatgg cggaattgct tttggaacag
    41221 attctgatgt tgcttttcaa ttaacacaag cagtaaagaa agtttcttct gttcctgtct
    41281 atgtcaaatt gtccccaaat gtaacagaca tcgttccaat cgctcaggca atcgaagctg
    41341 gaggggcaga tgggtttacg atgatcaata ctcttcttgg tatgcggatt gaccttaaga
    41401 caagaaaacc gattttagcc aatcaaacag gtggactctc cggaccagca atcaagcctg
    41461 tcgctattcg tttgattcac caagtagcaa gtatatccag ccttccaatc attgggatgg
    41521 gcggcgtgca gacagtggat gatgttcttg agatgtttat ggctggagca agtgctgtag
    41581 ctatagggac cgcgaatttc accgatccgt atatctgtcc taaactaatt aaagagctcc
    41641 ctgtgcgaat gagcgaacta ggcatcgaat cgctggaaag attaatcaaa gaagtgagag
    41701 aggaacgtga acgatgaatc aactagacca acgaccaatt attgcattag atttttcaac
    41761 taggcaagag gtagaagact ttcttcgatt tttcccgaaa gaagaaaaat tgtttgtgaa
    41821 aatcggaatg gaattgttct accaagaagg acctgaaatc gtacgttatc taaaagaggc
    41881 tggtcataat gtatttttag atctgaaact tcacgatatt ccgaatacgg tcgaaaaagc
    41941 aatgcgggga ttggcaaagc ttggtgttga tctcacctgt gttcatgccg ctggcggtat
    42001 tcgtatgatg gaggcggcaa tgcgaggatt agaagagggg actccagaag gtggaaaacg
    42061 tccgctactt ctagctatca ctcagcttac ttctacaagt gaagaagaaa tgcatgccga
    42121 tcaactaatc gaagtttctt tagaaaaaag tgtgatccat tatgctagct gtgccaaaaa
    42181 agcaggatta gatggtgtgg tttcttctgc ttgggaagta gaagcgatca aagaaatggc
    42241 aggagacgaa tttgtttgct taactccggg tataagacca gaaggaacag tcgcaggaga
    42301 tcagacaaga gtagtcacac cttctcaggc aaaaaagatc ggctcaactt ttatcgttat
    42361 aggtcgacca atcactcagt caactaatcc ttataaagca tatcaaacga ttcaaacaga
    42421 atggagctaa cctaaaatga atgttgaaca aagtatcgca aaagacttat tagaaattga
    42481 agcagtattt ttaaatccaa gtaatccttt tacatgggca agtggaatca aaagcccaat
    42541 ttactgtgat aaccgaatca cgatgagcta tccaaaagtc cgaaaagaga tcgcaaaagg
    42601 attagcaagc aaaatcaaag aagcttaccc tgaagtacaa gtgattgctg gaactgcaac
    42661 agcaggaatc cctcatgccg catgggtagc ggaaatactg gatttgccaa tggtctatat
    42721 tcgaagtaag gcgaaagatc atgggaaagg taaccaaatc gaaggacgta tatttgaagg
    42781 tcagaagatg gttgtgatcg aggatttgat ttctacaggc ggaagtgtct tggaagcagc
    42841 tgaagcagca aaaagagaag gtgcggatat ccttggtgtt gcggctatct ttacgtatga
    42901 gttgccaaaa ggaaaagcaa atttcgaaaa agccgaaatc cctcttatga cgttgaccaa
    42961 ttattctgta ttgattgaag cagctttaga agatcgttat atcgatgagc aagaattaac
    43021 tctgctaaaa gaatggaaaa aagatcctga aaattggcag atttaagcgc gcttgatgaa
    43081 gatagagaaa tccgatttgt gtctccagaa tcatcaattg gaatagcaaa aatccaaaaa
    43141 ctgattgcga gaagcatctt ttcatcaggt atgaaaagaa acttctatcg tgatcagttt
    43201 ttgatttttt taaaattcat ttttctaaga ctggttagca gaaagaagag aataatctct
    43261 tctctttttt ctattatata tagattttct ctattttttg gtaagggagc tgtttcaaaa
    43321 atggtaaaga gcggaaatgt tgcgaatatc agttatttaa tgatatgata ttcggaaaaa
    43381 gaaatctcaa agggaggtaa cttttatggg gttgaatgaa tttattttag gattgaacaa
    43441 tttagagacg atcacaaggg cacctggatt ttttaaatat acagagcata cagttgcagc
    43501 acattcgtat cgtgtagcat ctattgcgca agttctagga gacattgaag aagcgaatgg
    43561 acaaccgatc gattggaaac tgctctatga aaaatcattg aatcatgact acacagagcg
    43621 ttttattggt gatatcaaaa cgccggtgaa atacgcaagc aaagagttaa gagggatgct
    43681 gcaaacagtt gaagaaaaga tgacggacga attcatcaac caagagattc cagctgagtt
    43741 ccaagaagtt tatcgccgtc ggttgttcga aggaaaagat gaaaccgtag aaggagaact
    43801 attagccatt tcagataagg ttgaccttct ttatgaatct tttgatgaaa tcatcaaaaa
    43861 taacccggaa aaagtttata aggagatgtt cattgaggca gtgactacga ttaaaagctt
    43921 tgaacatcgg ccttctgttc agtatttctt taaagagata tttccggaac tattgaacca
    43981 ggaattttat ggaaaagagg cgtttttaga aaccgtctca acgattgtaa agaaagaaga
    44041 aaaataaaaa tgatatgaga tgcaggggga agtatagttg aaaaagaata tggatgtcga
    44101 atggggctat tctggagaca aaggtccgga aaattgggcg agtttatgtg attggtttgc
    44161 gagaggtgcg gaatttccat tgcagtcgcc tattcatttg accaaggggg aagaaaccaa
    44221 aatagaagga gacacgcttt cctttcatta tcaaaagcaa cggtttactg acaaggaatt
    44281 caaaaacacg attcatttag taccttttga ccaactcagt tatgtagagt tcaagaaaga
    44341 acgctattat ttgacagata ttcatttcca cttgccttct gaacatatca tcaacggaaa
    44401 acaggaagat attgaatttc attttgttca tatgaacagc aaaaaagaga atctagttgt
    44461 gggtgtcatg taccagctaa tggtacagga aggttggctg tataatcaag aaaatgggga
    44521 aagttggaac ttagaaaaac atgagcattg ggtaaatccg gatgtgttct ttccggagca
    44581 aaaaagccat ttccattata ttggctcatt gacaactccg cctaccagcg ggccaatcca
    44641 atggtttgta atggatcatg taggaaaact gaaccaagag tttcttctcc ccttcgatgg
    44701 tcagtatgct cgaccgaaca accgaccaat ccagcccttg aatggaagag aaatttatta
    44761 tttcgaggag tttgagcaat gaatattcag cagatgaaat acgtggtcgc tgtagcaaac
    44821 aatggtagtt tcagagaagc ggcaaagaaa atgtttgtct cccagccaag tctttcagct
    44881 ggtatcaaag aattagaaac cgagttaggg atcacgctgt ttactcgaac gaatcgaggg
    44941 gcatatttaa cagaagaagg gcaagacttc ttaaatcgtg ccgagaaaat attggtccag
    45001 cttgatacat tagaaactca ctatctaacg agtgaaaaga gagacaattt ttcgatcgct
    45061 gcccagcatt atgatttctt aggtcctttg accgcacagc tactcgagga attcaaagac
    45121 gagatcaaac aatttcggat catggagacg acaacagcga gagtgatcga agaagtcaaa
    45181 gagcagcaca gtgagatcgg cattatttat atgaatgagc ataatcaagc tggtatcaga
    45241 agatatttac aacaaggaga gctaagtggc cgcagcattg gaaaattcaa aacgcatatt
    45301 tttttaaggc aaggacatcc attagctgaa cacgcagaga tcaccaaaga agaattgtta
    45361 tcttatcctc aagttcgttt tacacaggat ggaaataatt tcccgtattt ttatgaagac
    45421 cttatcgaga tccctgatca agaaacagtg atttatacga gtgatcgagg tactttgatg
    45481 aatatcgttc ttgaaactga tgcttacgct tcaggatctg gtattgtgat tggagggatt
    45541 cgcgagcact tgcggttaat ccctttggca gaaggcgaac taaatgaatt ttatatcatt
    45601 tattcggcga aacgtccgct cagtgaaatc gctcaaagat ttatcaaaga gcttactcgg
    45661 ttactggacc agcagagcta agaaaagcag atgagaacgc tgtttattga acaccgttga
    45721 tcaaaaaaga ggggttgtga cagaattttg tcacaactcc tctttttgta gcaaacaatt
    45781 tataactaat tgctgttgtg ggaaagtacg caaatatgat tctagaaaca ctcattgatt
    45841 acctgttttg tttcatagct gtaatttttt cttcttctgg tgtcacgata atcgtctttt
    45901 ggttttcata aataacataa ccaggcttgg cgccatttgg ttttcggata tgctttacct
    45961 gtacaagatc aaccggcact tgggcagagt atcggtattt tgaaaaatag gcagctaact
    46021 cggcagcttc tgtaattgtt tcatctgagg gcttgtcact tttaatgatg acgtgcgaac
    46081 cgggtatatt ttttgcatga agccaatagt cggttttttt agctgtcttc attgtcagct
    46141 ggtcattttg aagattgttt ttaccaacaa gaatgagcgt tccgtcagaa gaaaagtatt
    46201 gatcaggctg agatggtttc ttacgttttt gtttttttaa gttcttcttt ttcaggtatc
    46261 cttctgaagt caattcttct ttaattgctt cgatatccat tgggccggca atttccagtt
    46321 gagataagac agattcaaga tattggattt catctttggc ttcttggatt tgttcgccaa
    46381 tcagcttaac agcgttcttc agcttttggt atcgatggaa atatttctgt gcattttgat
    46441 ttggtgtcaa tgccggatct agtgctattt tgattggccg atcttcttca taataattag
    46501 gtaaaacaac ttcttttgcg ccgcgtggta cttgtgtcag aaaagtagta agtagttcgc
    46561 catttctacg gtaattctca gcgttttcgg attctttgag ggtttgttca cgttttttca
    46621 atttattttt atttcgtttt agttcatttt cgatttttcg aatcagttct ccgccttgtt
    46681 gttttgcccg atctttctct gctttttcgt gataatagct atctaataat tgactgagtg
    46741 ttggataagc tgttacagca gatgcctgtt cggacaatac ctgataagca atcggtgtga
    46801 aaaaatcttt attttccgtt tcgtaaaaag taggaatagg ctgatggctg atggctgaga
    46861 aaaattcgtc ccatacaacc atcttttcat taggacgttc cagtaatctt ttggtcagct
    46921 cttgagcgat atcgaaaccg attccctgaa actgacgttg gattgctttt ggatctagtt
    46981 cagtttggga aagccgctga aagatttttt ctttctcgga gctaaatggg ttcaagacct
    47041 cttgttttgg cggagcaaca tattctacac caggaagcaa agaacgataa gtgttttgag
    47101 agcttccaat gtgtttgatc gcatccagta tttttccagt ttctcgatta actaagacga
    47161 tcgtactatg acgtcccatt aattcaacga tcaatatgat attttgtaga tcacccaact
    47221 cgtttcgttt agcaaagtgg aaatggatca ctcgatcatt ttcgatttgt tcgatgtctt
    47281 ctaaaatagc gccatccaaa tattttcgaa gcatcatcac gaagttaggc ggattatcag
    47341 gattctgata atcgatttgg gtaatctgta cacgagcata acttgggtgc gcagataata
    47401 ataatcgatg gtttttgccg cgtgaacgaa tcaccagcac gatttcattt tcataaggct
    47461 gatggatttt cgagatccgg ccagatagta acgtttcacg caattcgttg accatcgcgt
    47521 gggtaaatac accatcaaag gacatggttc attcctcatt tcttttcata agtatattca
    47581 ttataacgaa aaaaagaaga aaaaccaatt tggccgaata gtaaagcaaa agtctagaaa
    47641 aatcttggac taattacata aaaaacagtg tttctatcgt tgaaatccaa attttta
//
LOCUS       DACEUF010000008        46978 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.009, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000008 DACEUF010000000
VERSION     DACEUF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.009"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(<1..652)
                     /locus_tag="IXG20_000498"
     CDS             complement(<1..652)
                     /locus_tag="IXG20_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1608715.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIK"
     gene            complement(1220..2287)
                     /locus_tag="IXG20_000499"
     CDS             complement(1220..2287)
                     /locus_tag="IXG20_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1608716.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(2307..2786)
                     /locus_tag="IXG20_000500"
     CDS             complement(2307..2786)
                     /locus_tag="IXG20_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAQ1608717.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(2803..3264)
                     /locus_tag="IXG20_000501"
     CDS             complement(2803..3264)
                     /locus_tag="IXG20_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1608718.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(3278..4528)
                     /locus_tag="IXG20_000502"
     CDS             complement(3278..4528)
                     /locus_tag="IXG20_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ1608719.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(4906..6357)
                     /locus_tag="IXG20_000503"
     CDS             complement(4906..6357)
                     /locus_tag="IXG20_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1608720.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(6678..7442)
                     /locus_tag="IXG20_000504"
     CDS             complement(6678..7442)
                     /locus_tag="IXG20_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ1608721.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(7470..8168)
                     /locus_tag="IXG20_000505"
     CDS             complement(7470..8168)
                     /locus_tag="IXG20_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ1608722.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(8180..8959)
                     /locus_tag="IXG20_000506"
     CDS             complement(8180..8959)
                     /locus_tag="IXG20_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ1608723.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(8975..9919)
                     /locus_tag="IXG20_000507"
     CDS             complement(8975..9919)
                     /locus_tag="IXG20_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1608724.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(9965..10744)
                     /locus_tag="IXG20_000508"
     CDS             complement(9965..10744)
                     /locus_tag="IXG20_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608725.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(11124..13199)
                     /locus_tag="IXG20_000509"
     CDS             complement(11124..13199)
                     /locus_tag="IXG20_000509"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ1608726.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(13201..14118)
                     /gene="glyQ"
                     /locus_tag="IXG20_000510"
     CDS             complement(13201..14118)
                     /gene="glyQ"
                     /locus_tag="IXG20_000510"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1608727.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(14504..15322)
                     /gene="recO"
                     /locus_tag="IXG20_000511"
     CDS             complement(14504..15322)
                     /gene="recO"
                     /locus_tag="IXG20_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ1608728.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(15465..16364)
                     /locus_tag="IXG20_000512"
     CDS             complement(15465..16364)
                     /locus_tag="IXG20_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ1608729.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(16379..16780)
                     /locus_tag="IXG20_000513"
     CDS             complement(16379..16780)
                     /locus_tag="IXG20_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ1608730.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(16758..17234)
                     /gene="ybeY"
                     /locus_tag="IXG20_000514"
     CDS             complement(16758..17234)
                     /gene="ybeY"
                     /locus_tag="IXG20_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ1608731.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(17263..19455)
                     /locus_tag="IXG20_000515"
     CDS             complement(17263..19455)
                     /locus_tag="IXG20_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ1608732.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(19479..20450)
                     /locus_tag="IXG20_000516"
     CDS             complement(19479..20450)
                     /locus_tag="IXG20_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ1608733.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            21155..22534
                     /gene="brnQ"
                     /locus_tag="IXG20_000517"
     CDS             21155..22534
                     /gene="brnQ"
                     /locus_tag="IXG20_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1608734.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(22690..23136)
                     /locus_tag="IXG20_000518"
     CDS             complement(22690..23136)
                     /locus_tag="IXG20_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ1608735.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(23163..23339)
                     /locus_tag="IXG20_000519"
     CDS             complement(23163..23339)
                     /locus_tag="IXG20_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ1608736.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(23501..23929)
                     /locus_tag="IXG20_000520"
     CDS             complement(23501..23929)
                     /locus_tag="IXG20_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1608737.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            24021..24881
                     /locus_tag="IXG20_000521"
     CDS             24021..24881
                     /locus_tag="IXG20_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ1608738.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(24975..25268)
                     /locus_tag="IXG20_000522"
     CDS             complement(24975..25268)
                     /locus_tag="IXG20_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1608739.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(25341..27068)
                     /locus_tag="IXG20_000523"
     CDS             complement(25341..27068)
                     /locus_tag="IXG20_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1608740.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(27281..28207)
                     /gene="trxB"
                     /locus_tag="IXG20_000524"
     CDS             complement(27281..28207)
                     /gene="trxB"
                     /locus_tag="IXG20_000524"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ1608741.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(28352..29785)
                     /gene="ascB"
                     /locus_tag="IXG20_000525"
     CDS             complement(28352..29785)
                     /gene="ascB"
                     /locus_tag="IXG20_000525"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1608742.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(29785..31188)
                     /locus_tag="IXG20_000526"
     CDS             complement(29785..31188)
                     /locus_tag="IXG20_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1608743.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            31418..32320
                     /locus_tag="IXG20_000527"
     CDS             31418..32320
                     /locus_tag="IXG20_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1608744.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(32361..34247)
                     /locus_tag="IXG20_000528"
     CDS             complement(32361..34247)
                     /locus_tag="IXG20_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1608745.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(34344..35196)
                     /locus_tag="IXG20_000529"
                     /pseudo
     CDS             complement(34344..35196)
                     /locus_tag="IXG20_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(35531..37315)
                     /locus_tag="IXG20_000530"
     CDS             complement(35531..37315)
                     /locus_tag="IXG20_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1608746.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(37315..39066)
                     /locus_tag="IXG20_000531"
     CDS             complement(37315..39066)
                     /locus_tag="IXG20_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1608747.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(39208..39432)
                     /locus_tag="IXG20_000532"
     CDS             complement(39208..39432)
                     /locus_tag="IXG20_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ1608748.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            39683..41269
                     /locus_tag="IXG20_000533"
     CDS             39683..41269
                     /locus_tag="IXG20_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ1608749.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(41335..43110)
                     /locus_tag="IXG20_000534"
     CDS             complement(41335..43110)
                     /locus_tag="IXG20_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ1608750.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(43321..44223)
                     /locus_tag="IXG20_000535"
     CDS             complement(43321..44223)
                     /locus_tag="IXG20_000535"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ1608751.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            44502..46847
                     /locus_tag="IXG20_000536"
     CDS             44502..46847
                     /locus_tag="IXG20_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1608752.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT        13475 a         9934 c         8106 g        15463 t
ORIGIN      
        1 ctttaataaa acttgttatt tttgaatgtt gcaaatatga aatatggaat atgatatctt
       61 cagatatata atccctttct gacggaaact tgatattgtg ctgttgaatt atctcagatg
      121 aatacagcgc atgccaaact gacattggca atataaaatc gactttataa ctcggctcag
      181 tgccaatcat tccaagcaac actttttcaa ttatagcgtt atttgtaaat atcgtactat
      241 catatcttgc tggctttgaa ataatattat tatcatcata atattcataa tatccagcat
      301 aacatgtgtc tgcattactc ctttttatac catttaacat tgcttcaata taattagctg
      361 caacaaaatc atcagaatca ataaaagcaa catattttcc ttttacatgt tcaagtcctg
      421 tatttcttgc caatcccaag ccagcattat ttttatgaat aatacgaatc cttgaatcct
      481 ttaatgcata gtcttcacat attttattag atgaatcctt agatccatca tccacaatta
      541 taatatctag gttcttatat gtttgattac acactgattc caaacaactt cctacatact
      601 tttcaacatt atatacaggt atgattacac taattagatc actcgaattc atccttctat
      661 cctttcactc acgttttctc aattagtcaa tgacggcatc aacgattttc ttaaattctc
      721 tgaaaaaaat agactctgat cttgatgatg tgaatgacat actatcttct tctagaaaaa
      781 ttatttcctt taaattattt attaaatcaa taatatgatc ctcgtaagta tgctttccct
      841 tggaataatg aaaatatact tttgtattac acaatattgc aattttattt cttaatatga
      901 ttatttcact cattcattac tgccacatat acacaactta taaccacccc ctgcaaagtt
      961 tagggtaagt tatcagataa tatcttcctc ttcatcatcc agaatacaaa atctatttat
     1021 ttatagaaat atcattaatg tttttatata atgtaccgtg cttgtactct tgctctatta
     1081 caagcactaa aacactacta ttcatttgaa ttcttacacg gataaaaatt tacatcgtct
     1141 atttcaatat tttttctctc tattttattc acttaataag ttcattacat aatcgaaaca
     1201 ttttttattt tccttgtcat cactccttgc tccctttttg atacagattt aaaattctat
     1261 caaagaaatt tccatcgtta aatttcgtta ttgccaaatc cttcgaacaa cgtgaaaatt
     1321 ttacgtattt atctttatca ttgattttta gtattgcaga tgtcaacgct gatacgtcac
     1381 caggttgaaa caaatatcca ttttctccat cacgtactgc tgaagaaata tctcctacat
     1441 ctgtagcaat tacaggcatt ccataagaaa tcgcctctag aatagatata ggcaatccct
     1501 cgttatagga cggtaaaatc atcacatttg atcttctata gcactcctcc tttttttctc
     1561 ccacaaccca acctgtaaat tcaaaatacg attgcaaatt atattcttct atcaattctt
     1621 tgagcgatat ttcttcatca cctgctccag caatctcaaa aataaacttt ttttctggat
     1681 attgttctgc aatgttttta actgcttcta ccaaatcgtg tacacctttt cgctttatca
     1741 aaactcccat aaacagaaat cggcacattt ttgaattcca ctttatggta tattctggaa
     1801 ctacaataga attttctaca acaacagttt tacaatttgg ctcaatttct ttaattatct
     1861 tcttccattt ttctccaagt acaatcgtgg aatcagactc tcttaacagc aagcgaatct
     1921 gattttgttt ttttacagaa acactatcat accatttttt aaattccgat ccatgaagat
     1981 ggatgattac aggaatattt aataatctaa accacttatt cagcacatat ttcctactaa
     2041 aactccctct gtacgacatg tgcatatgaa taatattagg tttactcaat aaagagatac
     2101 ctataatttt agcaaatgca tacataaaat ataaagattt aaatattgca tttccaccct
     2161 tataactcgg tataaaagtc atctcgatat tctttttttc ccaattaaaa ttgagcattt
     2221 gtgtaattat acttgtaatt ccacctttta ccgagctgtc attaccaatc ataagtactc
     2281 tttgcataac gctttactcc ttatatttat attttttcta ttaatacctc tatcgaatta
     2341 ataatcgttt ccgtgttaga tacatacttg ttaaacatcc gaactcttgt ttctttaacg
     2401 gcatcttcta aattttctaa ttcataacaa ggatagataa tattcatctc atcaaattgt
     2461 ctaattaact gcagttggtg atcatcgaca tgctcaccat acttagccaa tcgaggaaca
     2521 gcaatgaccc ttttaccttt ttttacggcc ccaattatag cacctgtacc tccatgagta
     2581 atcaccattt ctgctttttg catagtttca gtaaactcat ccctatttag aaatgcttta
     2641 taggaataat ttagtggttt ataatcacta taaccactct gtgcaaatgt actttcgatt
     2701 attactcctt tttcaattaa atcatcaata gcttttaaca atctatcaaa ttgaaatttt
     2761 tgtgaaccta atgttataaa tatcattttt ttctcctatt atttaatata tcccacccaa
     2821 ataaatagca ttaggataaa attgtagcat agtttcccac tgaacataaa attgatctgc
     2881 aaaatgatac atcaattttc ctgtctgagt agggctggtt acttttgcaa acgattcaat
     2941 ataaatcagt ttttttccca tcatttttgt tattaagcag atagggatca tagctaaaac
     3001 acctgtacaa atcacgagat ccggtttttc ctttatatat ataataattg atttgaataa
     3061 attaataagt atagtacaca aaaaaaactt ttcttttcta tttacttgtc tcatgtagta
     3121 cgttttttcg ccttttgact cagctgaata ttctgtctgt tctgttataa caaaactctc
     3181 atacttctcc atcagtggtt ttaacatcat taactgttca taatgccccc cagaggaagc
     3241 cgcaaaacat atctttttgt tcatattaac ctctctatca gtcacgctga aaaatatctc
     3301 tagtgtaaac tttttcttta atatcatcca agcaggtatc atatcgattg gcaataatag
     3361 aatcacacat cgccttaaac tgctctaaat cgttgataac cttacttccg aaaaaagtct
     3421 ctccatcttt cagtgcaggc tcataaatca ccacagtcgc acctttagct tttacgcgct
     3481 tcataactcc ctggattgaa ctctgacgaa aattatcact gttgctcttc atggtcagcc
     3541 ggtatatacc gattaaaact ggattttccc tgttgacatc atactgatta tattcagcgt
     3601 agttgtaata gcccgccatc tgaagaacac gatctgcgat gaaatcctta cgggtacgat
     3661 tagattcaac gatagcctca atcaaatttc caggaacatc tgcatagttt gccaacaact
     3721 gcttggtatc ttttggcaag caataacctc catatccaaa ggatggattg ttgtagtgag
     3781 ttccaatacg aggatcgaga caaacaccat tgattatctg ctgagtattc agccccttca
     3841 tttcagcata agtgtctaat tcattgaaat agctgactcg cagtgctaaa taggtatttg
     3901 caaacagctt tactgcttcg gcttcagtga atcccataaa aagagtatcc acgttctcct
     3961 taattgcact tttctgcaga agtgttgcaa aaacatgagc agcatttaac aagcgctcat
     4021 cagtcatatc agtaccaaca ataatacgag atggatacag attatcatat aaagccttgc
     4081 tctcgcgcag aaactctgga ctaaaaataa tgttcttact ccccgttgtc tcacggactg
     4141 attcagtgta gccaacagga attgtagact tgatgaccat gatggtatca ggattcacgt
     4201 ccatcacaac ctcaataact gactcaacag cagaagtatc gaaatgctga gtacgactgt
     4261 catagttagt cggtgtagca ataacaacga aatctgcgga tttataagcg ctccttgcat
     4321 ccaaagtagc agttaagtca agttcttttt ctgtaagata cttttcaata tattcatcct
     4381 gaattggaga cttccgttta ttaatcagtt caatcttttc agggataatg tctaccgcgg
     4441 ttacctggtg atgctgcgcc aatagtgtgg cgatactcaa accaacataa ccggtgccgg
     4501 ctactgcgat gttgtacttt ttcttcacaa tatctcctct ttcctctctt aagtatggag
     4561 aatgttactc ctacggctat tcatcatgtc aaaataacac agcttagaat gttgataaaa
     4621 taattttcca gaatgtgata aaatctcttt catttataaa ccacacatct aattttatct
     4681 attctccaca agacatgtag agtattcttt aataacaaga tacgacctta ctaaatactt
     4741 caaaaaatta tcatatttaa ttcataaata taaatcttca ttttaaatgt atgcccaaaa
     4801 atttatctgt aatgcgtcat caaaatctag attccaaatc tttatcaatt tttatccact
     4861 cattctacca ctttagtaat attttctatt tataacactt tcgtcctaca ttgatccccc
     4921 cctctgaagt acagttttta ctgtcttaaa taaaatttta aaatctaacc ccatactcca
     4981 attttttata tattccgtgt caagttttgc tacttcttca aaatcagtaa tatcacttct
     5041 accacttacc tgccacattc cagtaattcc tggcttgata gcaattcttg cacgatgatg
     5101 ataatcatat tcttcatact ctgatttaag gcatggtcga aatcctataa ggctcatctt
     5161 tcctgttaga acgttgaaaa attgagggaa ttcatctaaa gacgtggatc taataaattg
     5221 accgattcct gtcttttttg ttccatctgg aagtatttta tttccgataa ttcttggatc
     5281 aaactccatc ttaaacataa gattattgct catcttattt tctttcatta attctacttt
     5341 tctttcttct gcatccatat acatacttct aaacttatac atcttgaacg gtttaccatt
     5401 ctgacctata cgttcttgtg caaagaaaat tgggcctggt gaattaatat aaataagtgg
     5461 agcaataaag ataaaaataa ttcccgttgc aatacatcca acaatacctg caagaatatc
     5521 aattgttcgt tttataaatg cctgttgagt tgtcataaca ttgatacttg ttgtaagtac
     5581 tgtatattct cctatacgac caatagattg ttttcccaaa gcactatctg atacttttgc
     5641 aagattcaaa tgtacagtaa ggcccatttc agagcatcgt tcaataaatt ctttcataca
     5701 cgaagtactt ttattcaaaa taataaagac ttcatctacc catccctgac ataagtaatc
     5761 cgctgcattt ttggaatcag aaacaactgt cactccctca acatcttcac caatcatgct
     5821 tttatctatg ataatgacac catttatttt atacatttta tagttatgct ctttcacatt
     5881 acgaataact acttcagcaa tactactaga tgtaactata ataagtgatt tgttcccctc
     5941 atttttcatt ttatgtttca agtaatgttt ccataagagg cgagttatat atgtaattac
     6001 aacgtataaa cctcccatca agaaaagaac gactctagaa taatcatttc caccctgaac
     6061 actaatgaga tacaaacttc ctaacagcac caacataatc gcttgcttta cagaagaagc
     6121 aaattctata tagtaccctc tttttaatac acctctatat gcttcaaaca tgaaaataac
     6181 taaaagatct gccagttcaa tgaagactgc catgttttga tacattggaa tcacataagg
     6241 attccaatct acctggcgta agacatacga tagataaaaa gcaagctgga tacacaataa
     6301 atctaaaata ataaaatcaa tatgtttaac ccatcccctt gaacttttct tgtacattcc
     6361 tttttactcc cctcttttta ataaaataaa ttgtattact tgttttctct cctaaatatc
     6421 tttctgaagg attattgact cgacaaattg gcttgtggat attcttatat atagaacatc
     6481 cgtaagccaa cttaaaactc cttaacaaaa tttttttact catattgtag atcaaaacaa
     6541 gtatccaaga aatcatattt tatttctttt ttctgcttac tcaacaacag ctaaaagaaa
     6601 aatcatcata aacaaattca actaagccaa cgatcccctt gtgaaatcaa gttttttctt
     6661 gatttacgaa taaagagcta aaataaacca aacttcttct tttttacctc ttcatagact
     6721 ggataactga tttcatcccc gttgatcaga tcctttgcga cttgtttcat ctgtactact
     6781 ttttccttac caaagtcctt tgcaatctgc tcatagcact cttttaagta aaaggtgcgc
     6841 ttagagaccc catgggcatc agaagcagcc atctgcacga gattatgttt gaccatcgtt
     6901 ttggctgttt tctggatgtc tttgccaaat accccaacat aacttggcgc agtgagttgg
     6961 gccaaacatc ccatatctag gaacggaagc aaatgatttg ggtcttcgcg aaaatgcacg
     7021 ttgcgttctg gatggacgat tactggtgtc tttcctagtt ctaacaactc aaaaaaaagc
     7081 cgttctgcat ataacggcac ttccagtgtc ggaaattcga tcagaagata tgtatcatct
     7141 aaatctgtaa ataatatttc atctcggtgg atgtcttcga tgagcgttcc ggtaatacgt
     7201 acttcttgtc cttccaataa cgtcaaaggc aactgtcttt tctctaattc ggcttgtaac
     7261 gaagcgacta gagaaatgac ttgtgatttt tcattgctgt attttccatt attgtggtgt
     7321 ggtgtgcata aaatatgcgt gatgccttgc tgaatcgctt tttcagccat tgcaatactt
     7381 gcttctaagt tttctgcccc gtcatcgatt cctggtagga tatggcaatg tagatcgatc
     7441 atttctcttg tctcctctct tcttattcct taatttccgt agtaataacc agcatctttg
     7501 ctatgttctg ctccgttgta cacaacacct aagactcgtg cttgtaccat ctccagcagt
     7561 tcttttgctt tagtcaagga ttctttacgt gacatatttt cacgtacgac caataaagtg
     7621 ccatccgttt ttaaagacat gatctgtgca tctgttacgg ctactactgg tggcatatcg
     7681 aatatcacca cgtcaaataa ctgtcttgct tcggatagga tctgatccat tcttggcgag
     7741 cttaacaatt ctgatgggtt gggtggctta ggtccactag gcaaaatcga caagttctcg
     7801 actggcgttc gttggatcac atctgctaca cttcctgatg aactaagtgc tgtacttaat
     7861 ccactggcat tgttcagctg gaatgtctta tagacgacgg gtttacgtaa atctgcatcg
     7921 actagtagaa ctcgttgacc tgatttagca aaaacgactg caatgtttgc tgcagtggtg
     7981 gatttccctt ctccaggacc tgatgaagtc acgacaatcg ttttgatttg ctgaccagct
     8041 gcggaggcaa actggatatt cgtccggatc gtacgatatt gttcggcgat tggtgaagat
     8101 gggtttgtta atgtgatcaa actgactgca tgagtttgtg ctgctttttg tttctgtgtt
     8161 cgtgccattt ttcttcctcc tagactcttg agcggcttct gcgtgatggg gctgttttat
     8221 cgtttgtagc ttttggtcgt gcaggagctg ttcctttttt acggatacca gcgttcaatt
     8281 ctttcgctga catttctgga actgttccta agattgggaa tcctaatgta tctgtcacat
     8341 atttttcatc tttcactgtc cgatcgagta attccaataa gaatgccaga cctacaccta
     8401 ccatcattcc taacattaag ccagtcatta aattcaattt attatttggc gaaacaggtg
     8461 tcgtattggc aactgcacct gaaatgatcg agattttatc aacattcatc acatcttttg
     8521 cgttctcttg gaaaacttgt gaagttgtgt tagcgatctg ttgagcagcc actgcattcg
     8581 tacttattgc ctgaatcgag aacatttgtg aattttctgc ctgattaact gagatggcat
     8641 ctttgatttg ttcagcagtc attttcacat tgtcttctgt ttctaaacga tctttgactt
     8701 cattgagcac taaatcactg acgatcatat ctttatacgt attgatcatt tgcaggttcg
     8761 tattgacatc attgacattt gcagtgtctg attgtggcag tgtcacgatc aattgtgctt
     8821 gtgaactgta ttttggtgtc aagatgaaaa atgttacgat tcctgagatg gccaagccag
     8881 caaacattga gatgatgatt attaccaagt gtttgcgtaa tgtttcaaaa atttctttca
     8941 agctgattgt ctcttccatt ttttctccac tttcctattt attctctaga ttcatttgat
     9001 cttttaattc ttttttcaca cgatcaagtt cttgatcact gactttttga taggagatat
     9061 tatcttgcat gaagccttct ccttgcagct gatcttgctt aatcgtacca aatgcctccc
     9121 gataatctaa agcaattttt ttcatttcat caaagttcat gtctgttttc atattcgact
     9181 ctagagcatt caaaacggat tgatagttgc tgacaccgtt tagactcaat acttttttcg
     9241 ctacgccttc tacgatcttt ctttggcgcc cttgtctacc gtaatcacca tttgggtctt
     9301 cataacgcat acgagaataa gataatgcct gatccccgtt caatgtaatt ttaccgattg
     9361 gaaagtcata gccatcttgt gagaaagtga ggtcattatt gacttcgata ccaccaactg
     9421 catcaacgag ttcctttagt cctttcatat tgatagagac atagtgatcg attgggatgt
     9481 ctacatattt ttgtacggta tccatcgcca tggcagctcc gccgaatgcg taagcgtggt
     9541 tgatcttgtc ttttgtgtca cgacctacga tgtcgacata cgtatctcga ggaatgctga
     9601 cgatcgttgt ctgtttgtct tcaggactga ctgtggcaac catcatcgta tctgatcttc
     9661 cttgatcgac ccgacctaaa tctccagtat caatccccat caatagaaca gagaaagatt
     9721 cttgtttgtc cagatcaact ggtttcgttc gttctccttg ggttcgttcc actgattcgt
     9781 aagttttctc aatagaatgt gagagatcca gatataattt tgctcctact cctacaattg
     9841 ctaatactaa tacgataaga actgctaaaa taatcaaaaa tatttttttc cctcttgatt
     9901 ttcttcttcg tcttgccata gtttcctccg tttgctaacc ttgcttgaat caacgtttta
     9961 actgtcaata gcttatattg ctatagccca tttgctgagc aacagtttgt gcttttgttg
    10021 cgtatgcggc tcttattttt tcattccgga tctgctctat ttgactgagc gccgtcaaat
    10081 attgatcgta agtagcggta gctgttactt gaccgtcttt cagcatcgta tcgaatagct
    10141 gatctagttg cgtgacttgt gcaatttggg tatcagcgaa tcctagatat tgcataacga
    10201 ctgttttcca agcactatcc ttaaaaaagc tcatattttc acgtacatgg gcgacatctg
    10261 cacttgccaa cttttctttg atgatgacat catccacagc ttgctgccag tttgatacgt
    10321 ttttagtaaa taatttattg acagccgtct ggatcttcag ttttgattct gcatcctcta
    10381 actgcgttaa gactgctttc ttttcttctt ggatcgcagc agcttcttta ggtaattctg
    10441 attccttgat gtcataatca tctgccgaaa gtttgataga agatagttta tgtgacaaat
    10501 ttgaaactgt ttcttctgtt atttcaggat ttaggaaaat tttttctttt gtactgtaca
    10561 gcttatctac ttcttttgat aaagaggtca cggtgtcttt ttgattggtg atggcagatt
    10621 ctgcgtaatt gatccgttga tgatattgcc gctgattatt gagatggtat ccaaacaagc
    10681 aaatcacaat agcgcctgtg cctaccgcca cagtagccaa taacttgacg aacggctttt
    10741 ccatagattc actcctaaat attgatgata gataagtaaa gatgtgattc attcacatca
    10801 gaatgagcgc tttttatttt aaaagcataa ttaaactaat atattcatag aaatatttat
    10861 aaacaggttt tccctatata atcataccac atacagtttt tttgactagg gaaattccta
    10921 atttaaaagt aaataaaaca gtaacaagat tataattaat cgttttcatt tttatttcct
    10981 cacttttcat gagcgttttg aaaattcgaa caaagaataa aataacaaaa attattaaaa
    11041 tagaattaat aacagctatt tataacaaaa aaaacagaga cacagcacgt ttcgtgcatg
    11101 ctctctgttt ttcaaacaaa agactatttt gtgatgattt gatccaagct tgctaatgac
    11161 aaagccatgg cagctatttg acttagttgt gttagtcggt tcttacgtac tgctggttca
    11221 tcagccatga ccatcgtttg atcaaaataa ttttcgatca gtggacgaag agcgacaagc
    11281 tggtcgtagt tttcgtggat cgtttggtca gcaaaacttg ctttcattgc ttctactgct
    11341 ttgtgtaatt ctttctctgc gtcgttttcg aacagctcag ggtctacttc tgattgtttt
    11401 tcaagctgcg cttttttcgc taagttcacg acacgtgtca aagcttccat tgaaggtttg
    11461 aagtcttcat ctgcaagatg ttgtttgaac aattgtgctg ctgcaaatac ttttgtcaga
    11521 tctttttgtt ccgcattcaa aacagcatcg atgacatcat gacgaatatt cttcgttgtc
    11581 aataattggc gcagacggcc tttgataaag tcgatcactt gttgttgccc ttctgataaa
    11641 ccaatgccga aacgatccgt atctttactg ataacatcgc taatcgtttc ttgcagaaca
    11701 gacaaaggaa aggcccattt tttcgattcg atgatccgaa cgacaccgta tgcttggcga
    11761 cgtaaggcat aaggatcgtt tgacccgctt ggaatcatcc caactgcaaa gaaagaaaag
    11821 atgctatcta atttatctgc aatcgctaaa acagaaccta ctgtagactg aggtagctct
    11881 ccttcactgg atataggcat gtaatgctca cgaatcgctg tcgctacagc aggtttctcg
    11941 cctttcagaa gagcatattt ttcgcccatg atcccttgta gttcaggaaa ttcccctacc
    12001 atgttggtta ctagatcaaa tttatagatt tcagcagctc gttgcaaatc catcaattct
    12061 tcctcagata gtcctacttg ttcgccaatg atacgggcaa tgacttgtac ccgttgcatt
    12121 ttctcataga tactaccgat tttttcatgg aaagtaacag tttttagttt ttcaacgtat
    12181 tggtcgattg aagctttctt gtcttcatta taaaagaatt ccgcatcttc taaacgagcc
    12241 gtcaatactt tttgattgcc tcgaaccaca tcttccaatt ttacccgatc accattacga
    12301 gcagaaataa aatgaggaag cagcatacct tgatcattcc gtacctcgaa ataacgttga
    12361 tgttctttca tagaagtgac tagtacttct tcaggaacag aaagatattt ttcttcgaag
    12421 tcgccagaaa agacagttgg atattcgact aaattgttga cttcttctag taaatcttcg
    12481 tctagatcaa taacccagtg atttttttct gctaattcgt ttgcttgtgt aacgatcatt
    12541 tcttgtcttt tttgcggaga cgcaatgacg aattgttcat tcaatttttc ttcatattct
    12601 tttgcatgcg caaatgtcac atcttcgcct aagaaacgat gacctcgaga ggctctgcct
    12661 gttttgacat ccaagatttc gaatgggatt acttcgtcat ctaacaatgc cacgatccag
    12721 tgaatagggc ggatatattc aaaatcgtat tctgcccaat gcatcgttac agggaaagtc
    12781 aaacttttga tcacccctgc taagcctgtc aatacttctt ttgcttcctt accatgagtg
    12841 tatttcgtta catagacata ttccacacct tttaattctc taaaagtgat agcgtctgtc
    12901 gtcagacctt gtccgcggac aaatccttcc gctgctttcg tccagtttcc atctgcgtcc
    12961 aaagcaattt ttttggctgg tcctttgact tcttcttcac tgtccgcttg tcgttctgga
    13021 atatttgtta ctttgattgc taaacggcgc ggtgtggaaa atgtttcgat cgtatcataa
    13081 gtcaattggt tttcatctaa aaattttgct gttttttctt ctaattgttt catgcttggt
    13141 gtgacaacat gggcaggcat ttcttccagc ccgatttcaa gtaataagtc ttttgccatc
    13201 ttattcagcc tccttcacgg tttctggatt tgctgtgttt ttattcaaca aagggaatcc
    13261 taatttttcg cgttctgcca caaagatttt ggctacagca cgagccatgt tgcggatgcg
    13321 agaaagataa ccggcacgct ctgttacgga tactgcaccg cgggcgtcta ataagttgaa
    13381 tgtatggcta catttcaaga tataatcata cgccggatga acaagatttt catcgataca
    13441 gcgtttagct tctttttcaa aacgatcaaa gttgcttagt agcaagtctt ggtcgcttac
    13501 ttcaaaagaa tatttggaat gttcatattc tggttgtttg aagatctcgc catatttcac
    13561 gccatcagcc cattctaaat catagacact ttcaacttct tggatataag aagcaagacg
    13621 ttctaatcca taagtgatct cagaagttac tggtttacaa ggcagtccac caacttgctg
    13681 gaagtacgtg aattgtgtga tttccattcc gtcaagccat acttcccaac ccacaccggc
    13741 acagcccatg gatgggttct cccagttgtc ttcaacaaaa cggatgtcgt gttctaatgg
    13801 atcgattcct aaaaggcgca agctttctaa ataaagctct tgaatattct caggtgaagg
    13861 tttcatcacc acttggaatt gatgatgctg atacaaacgg tttgggtttt caccataacg
    13921 tccatcagct ggtcggcgag atggctctac gtaagcagca ttccatggtt ctggtccgat
    13981 tgcccgtaaa aaggtatatg ggctcatcgt acctgctcct ttttctgtgt cataagcttg
    14041 catcagcatg cagccgtttg atgaccaaaa tttttgtaat gttaaaatca tatcttggac
    14101 ggtcaatttt ttactcatct gttttgcccc tttcaatttt aaagcgttgc tgtcaaataa
    14161 gaatacagga aagaaaacga caaaaaatcc ctatgtctga ttacagacat agggacgaat
    14221 aattcgcggt tccaccctac ttccgaataa agattcggca gcttcattta tcgtactcgc
    14281 aagtgcctct tcaaaaatcg tttgggtccg gctcccactc tcccgaactc gctaaaccac
    14341 gtgtttttta ctgatcttgg tcaatgtacg tattcatttt gcagttttct tgtttctata
    14401 atagatccta tcactgaacg atctatttaa tctatggtta ataatagacg gaactccctg
    14461 tgtttgtcaa atggttttga ttttttgttg tatgttttat ttactattct tctttttcag
    14521 gtgtctcttt ttctttttca gacatgtctt tctctgacgt ctttaatacg tgtgcccaag
    14581 acttcatttg attgatgaac tttttgcttt tcaaatggat accgacatat tcatcataca
    14641 gctggtcgat gagttcacgg attgcttgtt tggtttcttc tttcagatgg attccactaa
    14701 ttttatcata agaaatagct gaaaacatcc gaacgaaata aacagctcga ggatctgcat
    14761 gataacggtg atcgtccatc gaccaatgtc tttcgcatat cacaccacca tatttggaag
    14821 aatagtcaaa ttttcctttt gtttccccgc atacgacgca gtgtgtccag attggtgcaa
    14881 ttccgaagcg ttgcagtatt tggacttcaa aaatattcgt gatgatttct gcatcgtctc
    14941 cttggtccat ccgttgcagt gcttgttgcg taaactggaa caaatgcggg tcgtactgat
    15001 gatcttcgat tgccgcatct actagattca ataaatatgt gccatagccg ctcaaaaaga
    15061 tgtcttcttg gatgtgtcga aacgcatgga catctttgac actgcttaaa aatgacaaac
    15121 cttcacttcg aaaattcccg atataaactg cttcagtaaa agggagcaaa gccggagcta
    15181 agggctgatt ttgccgatgg atctctttga tataaaacat cattttgcca gaagattctg
    15241 taaagatttt gactaattta tctttttctt tgtaattacg gctagataaa atcaagcctt
    15301 ttgattctgc aagctggctc atattctccc tcctaaaaaa taaatctgga aaagcagatt
    15361 ctgccgatcc agatcattta tgttgcgatc ttccgcagct tttctcaaca cgcggatttt
    15421 tattcagttt atttctatac ctaatttaca agaagctcag aatatcagta ttcgtctttt
    15481 cggtagccga agtcttgcag atagactttt ttgtctcgcc agtctttttg aactttgacc
    15541 caaagttcta gaaagacttt gtctcctaaa agattttcga tatcttttct tgctttcgtt
    15601 cctacttgct tcagcatttt tccgcctttg ccgataatga tccctttttg gctatctctt
    15661 tcgacaataa tcgtagcctg gatatggatt ttatcattct cgtttcgctt catcgaatca
    15721 acaacgactg cgacagaatg aggcacttcg tctcgtgtta aaaacaagac tttctcacgg
    15781 atcaattcag aaacgatgaa gtattcagga tgatcagtca cttgatcatc tggaaaatat
    15841 tgtgggccct ctggcatttc actgactaat acatccatca aacgttcgac gttgtttcct
    15901 tctgtagcag agattggaat cacttgtgca aaaggcatct gcgttgaata atcttcgatg
    15961 attcccaaca actcgtctgg atggattttg tcgattttat tgataatcag ataaacagga
    16021 gaatgcacgt tttttagacg ttcaataata aaatcgtctc cccgaccgcg tttttgatca
    16081 gcactaatca taaataagac cgcgtccact tctttcaacg cactgtaagc tgcttctacc
    16141 ataaaatctc ctaaacgatg ctttggttta tggattcctg gtgtatcaat aaaaatcaac
    16201 tgagcttctg gtgtagtgta tactccttgg attttatttc tagttgtctg tgccttgtca
    16261 ctcatgatgg caattttttg acccacgatt cgattcaata atgtggattt ccctacattt
    16321 gggcggccga cgattgcgac aaaccctgat ttatgttctg gcatatatgt tctccttttt
    16381 aattaaacaa caaatgatat attttaggga caaataaaat caacccaacg atgacggtaa
    16441 aacaagcggt cactaaaact gctccagctg ccatatcttt gatttttttc ccaatcggat
    16501 gaaaatgaaa atccgtaaac atgtccacga cattttcaaa aacagtattg atgatctcta
    16561 ctatccatac gagaaaaaca gctaaaagca gccataacca ttctatccga tcaagttgga
    16621 aaatcacgcc cataaacaaa gcgaggattc cgaacgcaac atgtttcctc atgttgcgtt
    16681 cttcctcaaa aacagttttg actccttgga cggcaaattc cacggacatc aggaaatgtt
    16741 tgtttttctc tacgttttta tcttttaagt ccataggcat ctaagatctc tttctgtaaa
    16801 ccaaacattt ctttctcatc ctctggtgtc atatggtcat acccgttgat atgcaagaat
    16861 ccatgaaccg ctaaaaagcc aagttcccga tcatacgaat ggccgtaagc ttctgcttgc
    16921 tctgcagcac gttcagtaga gatcatgatg tctcccagta cacgaggcaa tggattctct
    16981 ccatcttcaa aaatcactgg gatttcgtct tcgccctctt cttccaaggc aaaactgatg
    17041 acgtctgtcg gtgcatcttt tccacggtaa tcacgattga ttacttggat tgctgcattg
    17101 tccatgaaag taacagacat ttctgtttct tctggcaact ctaaatatga tgctgcaaat
    17161 tgcagcaagt cgtcgatttc tttcattttc tcttctgaga cttggtttgt ttcatccatg
    17221 aaggtaatat ccattataca tcgcttcttt ctattcttct tgctattctt cttgttcttc
    17281 ttttatcttc tcaacttccg atttcatttt aggatatttg atccgagaat ggaaggtact
    17341 gcttaaaccg ctgatcaatg atttttctac tttacggatt tcttttaacg tcaatccact
    17401 gtcgtccaat tgtccatcgg aaatacgctc ttcaataaga ttatggacaa aactttcgat
    17461 tttttcgctc gttggatggt ccattgcccg tactgccgct tcacaactgt cagctatatt
    17521 gacgattcct gcttcacggc tttgaggttt tggtccagga taacggaatt gctcttccgt
    17581 aacttccggg ttgcgttcct ttgccttaac gtagaaataa cgcatcagcg tggttccatg
    17641 atgctgtcta caaatatcga tcaccatttg cggcatatta taatcttcca atattttggc
    17701 accatctgtc acatgtccaa agataatatg tttactgtct tctggcaata agaaattgtg
    17761 cggattttcc gctcctgaag gcaagttctc aacgaaaaag ctggcgtgtt tgatcttgcc
    17821 gatatcatga taatagcaag ctactcgagt tagtaaagaa cgtccaccga tttctgctac
    17881 agcattagca cttagattgg ctaccatcat gctatggtga tacgtacctg gtgcttcttc
    17941 cagcaactgt ttcaataaag gatgattggg attgctgagt tcatttaata cgatcacact
    18001 atcatcattg accatcaatt caatataggg gtgcagacct atcgtcagca agaatgaaaa
    18061 cagtgcacct gtgaatccgc agactagcgt taaccaagtc gttgcatccc caaagctcat
    18121 tccttggtag ataatcaaaa tgacatccat catcacagga aaaataatga cccacatagc
    18181 agcggataac ccttgttctg agatccgttt gcgtctgacg atcgttgcca tgagaccaga
    18241 aaatacatag gacatcaaaa taactgtcag aaagttcgtg ccaatcgaat cataaaaaat
    18301 gaaaagtgcg gatactgctt gaaaaatacc agccatgatc cctgctcttc gattcaagaa
    18361 aaagcttaaa acaagtggag caaaagccgc ggggtaaaac agcggaatat aagcagcttg
    18421 ttctgtttgg aagagttgga aaaatttcat tagtacgacg cttactgaca ttgccgtcac
    18481 gtagaaaagt acgtattttg ttctttctcc tgcattttgg aattgaagtg aattataaat
    18541 cagtacaccg atttgaagca aaacagctaa aaccatggca atgagtggga aaatagaagt
    18601 cgtttgattc gtcaatccta gtaattccaa ttttttcatc gctgaagcat caatttgatt
    18661 tccttcgcga acaataattt ctccttggaa gatcatcact ggttgtacag cttctcgtgc
    18721 agattgtttt aattcttctg tctttttctc attcaataca tcattaacta cgatgccttg
    18781 atccactaag tagcgaatca tttgttgctg ctctgttgtg acattcaaat attgaatctg
    18841 atcttctgct tcttgtttaa aagcttccaa ttcgctttca cgaacttgtt tactcatctg
    18901 ttcgtcaatg agcttcaaac tttcatccct aactgtgtga atttccgttt cagtcatttc
    18961 aaatatcgtc gaataaaaat tgttcggcag tttttggtaa aacgcaacgt tttctgcatt
    19021 cacgttttca aaatttttct tcagtgtagc aatccgctct tctgtagtag gtttcgggat
    19081 attttcatct tccttcgcct tatctacctt gccattataa gatttgttga tggtatcatt
    19141 cgtcttatcg atcaattcga ataattgctt gatccgattg ttctggtcat ctgccagatc
    19201 tttttgataa gtatattctg gagtcactgc ttctgctgcc agttttcttc tttgttcggt
    19261 ttcttccgtg ttctcaatcg ttttattcgc acggatactc tcttctgcta cttggccttc
    19321 tctgaaatca atggcctttt gtttgacact actaaatgta acgccgcaga caatgataaa
    19381 aaacaagaac agaaggaaag gcaatgctgc ctttccgagt ttttcttgta gtttctgcag
    19441 tgttttattc aacatgtgct tatcctccta agcaaagctt attcttgatg aagatccgcc
    19501 ttttcataag ctttgatgat ttcggcaact acagggtgcc gaacaacgtc acttgcttca
    19561 aaattaacaa aatcgatttt tttgatttgc tttaatgttc gttctgcatg aacaagacca
    19621 cttgtcgtcc cacgaggcaa gtctatctga cttgtatccc cattgacaat catttttgat
    19681 tgataaccga gacgggtcaa gaacattttc atctgagcga ctgtcgtgtt ttgtgcttcg
    19741 tccaagataa cgaatgcatc atccaatgtc cgcccccgca tgtaagctaa aggtgcgatt
    19801 tcgatcacgc ctcgttccat caatcggttc gtatgatcca atccaaaaat ttggtataaa
    19861 gcatcataaa caggccgcag ataaggatcc actttttctt tcagatcccc gggtagaaag
    19921 cctaggtttt cccctgcctc cacagcgggc cgcgtcaaaa tgattttttg cacttcgcct
    19981 ttttttaggg cagcaattgc taatactacg gcaagaaacg ttttccctgt tccggctggc
    20041 cctacaccaa atacgatgtc atgtttggct accgattcaa cgtatttctt ttgaccgctg
    20101 ttttttaccc gtataggttt tccattacgg tctttgacga tctcttcctc atacatctca
    20161 acaaaataat ctaatgtacc tttttgcccc attttcaatg ccgtcacaac atctggcgta
    20221 cttacgtgga ttcctcgttt gatcaattcc tgtaatgtcc gaatgactga ggcagccaag
    20281 gaaatctggg attgctcacc aatgatctga atgatttctc cacgtgtatg gatggttgtt
    20341 tccgttgttt cctcgatcag ttttatatgc ttgtcgtgtg cacctaaaag catgctgaca
    20401 tcatcagcag ctgtcaatct aatctctaaa gaactagact cttccgtcaa aaatagacat
    20461 cccctttttt tcgattcatt ctttcctatt ttaccatact cagaagaaac tatatagcaa
    20521 ataagaaaag gttgtaaaag agtcgcttaa ccatacgtac caagaaacgt taaaaaatag
    20581 ccgtttccat tcgctaaagg tctcgttttg ataaaaagaa gctgagaatg taagttatat
    20641 gaaagtggag aacaaaatat ttcgtttcat tcaacaagta ttctctaggt atttttcctg
    20701 tttcaaaaaa agaaaacaaa atagttaaaa tattcttgca tttcaaaaaa aacaggtgta
    20761 ttattacgtc aacaaataaa catacagcaa agcgtcgagg aaaacagtag gctattcaac
    20821 gatttattcc cagtgagtca tgtaagtgag aaatgacaat aaatcgaata gacgaacatc
    20881 atttccgagt caatgtcgaa aacgtagatc attgtggctg cctccattat caggctagcg
    20941 gataacttta tcaaaaagcg tccgtgaatg aagaagcttt tttaagcgaa aaaaggtggt
    21001 accgttctag aatctagagc ccttttccca agtggaaaag ggcttttttt tattttaagg
    21061 tggtggtttt catggaatct gatattagtg gcattactaa ttgctgcaac gtttatatcg
    21121 tcaatcaaaa tgataattat tggaggaaaa atttatggaa gaaacaaaaa cttattcatg
    21181 gaaacaacta ttgatggtca gttcacttat cttcgggatg ttcttcgggt caggtaatct
    21241 aatctttcct gtccacttag gtcaaatggc tggcgcaaac tgggtcagtg ctggcgtagg
    21301 atttgctatc tcaggtgctc ttttcccatt actagctatc ttagctgtag tagtgacaaa
    21361 aagtgatggt ctgtatgact tagctaaacc tgtcggaaaa tggtatgctg ccttgttctt
    21421 aatccttgtt catttgacga tcggaccatt tttcggtaca ccacggacag ctgctactgc
    21481 ttttgaaatg gctgctaaac catttttgcc tgaaaaatat aattcattag gtatgttttt
    21541 attctctgct cttttctttt tacttgctta tttccttact gtccatccaa cacggttggt
    21601 caaatacgtc ggaaagtatc taaactcctt gtttcttgca ttattagcaa tcatttttgt
    21661 cgtttcttta ttccatccaa tgggaaatct aaatcaatct gctgcagata cttatcaaac
    21721 aaacgcttta acaaatggtg tcctagaagg ttataacacg gttgatgcag tagctttgct
    21781 tgccttaagt gtcacttttg tccatgcagt ccgtgacctt ggtttcaaag ataaaaaagt
    21841 cacagaatta acggcaaaag ctggtagcct gagcgttgcg ctagaagtca tgatctattt
    21901 tggacttgtt cttctcggtg cgatgagttt gaataagatg tctttatcag aaaatggcgg
    21961 aactgcactt tctcagatca ctaattttta tttaggtaac ttcggcgcta gcttcttagg
    22021 agtgatggtc acattaggtg tcttcactac cgctatggga ctcgttgttt cttttgcgca
    22081 agatttccat aaactgttcc ctaaagtcag ttatatgaca tggctgcggt taacgacttt
    22141 cgtctctttt gtcgttgcaa atgctggttt agacaatatc atccaatggt ctttaccagt
    22201 attgatgctt ttgtatccat tgtcgcttgc gttgatcttg ctttccttaa cagcaaaatt
    22261 tttccaaaag acaccatttg tctatcaagt aacgatgttg tttgctgctg ttcctgccgt
    22321 attggatatg ctggcaaatt cgcctgcttt agtgagtcaa caaagagttg tagcaagcat
    22381 gctcgaattt tatcatcatc acgttccttt tgctgcatta ggacttggct ggatggtccc
    22441 aacgttactt ggttacgctg gcagcctact gttttattac gcctatcgcc tatcagggta
    22501 taaacaagaa gcaaatgaac taccagaaga ataaccaaat caaacacaat ttaacataag
    22561 aaatattaaa caggaaaaaa ccagctagat gaaagaatcc tattctttcg tctagctatt
    22621 ttttagttct cttttttacg cgcacaaaaa aactgcggac aatgattgtc cgcagtttaa
    22681 atgataaaat taagattgta atagttcttt cacaatggca tttacttggt ttccatctgc
    22741 ttttcctttg actttaggca tgatcgcacc catgactttg ccaaattctt tcattgatga
    22801 agcaccagtt tgtgtgattg cttcttgaac aagttgtcga atttcttcgt cggaaagttg
    22861 ttccggcatg tatttttcga ctatcacaat ttcacttttt actttttcag ctaaatcgtc
    22921 acgtcctgct tcttcaaact catgcaaaga atcacgtcgc tgtttcattt cgcgagacaa
    22981 caccgttaat tcttcttctt ctgtcaagtc gtgaccttct ttaatctgct cgttttgtat
    23041 agatgacttg atcattcgga tgacttgtaa agattcctta tctttcgctt tcatcgctgt
    23101 tttcatgtca tcgtttaatc tacttagtag tgacacacta ccaactccaa ttcatcacag
    23161 aactagaatt ttttgcgttt tctagctgct tcagattttt tcttgcgtct tacacttggt
    23221 ttttcgtaga attcacgttt acgagattct tgtaaagtac ccgcttttga cacggaacgt
    23281 ttgaagcgac gaagagcatc gtcaagtgat tcgtttttgc gaacgactgt ttttgacatg
    23341 ttaattccct ccctccgagc ttaaaaagta accgtaattg ttattgaaat atatctcaaa
    23401 aaacaagact gtcctgtaaa tattatatct aatgctgttt tgaccgtcaa ggaaatcttg
    23461 ctttttttta aaattatcaa atgttatttt atttaaatcc ttattgacaa ttttcacatc
    23521 ttccgaaaat ctcgaatcta tgcccttcga tcgtacatcc accgagctgt tcttcgaaat
    23581 aattcattgg acacatatgg atttctttcg ttgttccgca caccgtacag ataaagtgat
    23641 ggtgatggcc gatttcccct tgacagcaat gaaaacgaaa cttcatctct ccatttaaat
    23701 cggtttcttc cagtaaatcg atttctgcaa aatcatggag atttcgataa atcgtatcgt
    23761 aacttactcc tttgaattcc tgattcatat agtcaaaaac ttctttagcg gatacgtagc
    23821 gattgcgctt cactaaataa gttaacaatg cttctctttt tttagtatat ttaaaaccgt
    23881 tatttttcaa tgtttctagt gattgttcaa tcaatgtctg ttttttcatg acttattcct
    23941 ccaaatcaaa atgattacga agtacacatt ctagagtatg gtataataaa cataaaaaaa
    24001 atgcaagtga ggttttatcc atgtcttccg attctacaga aatcattact ttttttgtca
    24061 tctctgactc tgctggagaa actgcgacaa aattagcgca agccactatg gcccaatatc
    24121 ctaccgttga gttcaattta ttccgcagaa catttgtgac agatgagaaa acattgatgc
    24181 aggcactgca agatgcacta aaagaaaaag cccttgtcct acacacattg atcaatcaag
    24241 aattgatcga gatgacgcgt tctttttgtg aagaacatca attatttagt tttgatgtga
    24301 tgactccgcc gatatcagaa atcgagcgct taactggtat caagcccatt cggcaacctg
    24361 gcgcccttca tttattgaat gagaactatt tcaaacggat caaagcaatg gaatttgctg
    24421 taaaatacga tgacgggaaa gatcctcgtg ggtttctaga agcagatgtc gttttgctag
    24481 gcgtatccag aacttccaaa acgcctttga gcttgttttt agctaacaaa aatttaaagg
    24541 ttgccaatct acctttgatc ccacaagcac atattcccaa acagctttgg gagatcgatc
    24601 ctaaaaaaat cgtgggactg acgaataacc cagatatttt aaacaatatc cgtaaagaac
    24661 ggatgcgctc ttacggatta aatcctgata ctgcttattc cgatatcgaa aaaatacgag
    24721 ctgaactcga ttttgcgaat gatctttatg aaaaattggg ctgcgtagta atcaatgtcg
    24781 cttctttatc tatcgaagaa acagcttcac ttattttgaa tgctttagac ctagaagatc
    24841 atagttatta tggaactgaa accaccgaag aaacagacta acagtttttc ttgtcttttt
    24901 cttaaaaaaa ctggactgtg tcagcgaaga agattcgttg atacagtcca gtttgtctct
    24961 atttgcacct atcgttattg atgttccaca tgtttgagcg tctggttcaa aatatccagt
    25021 acgtgagaat catcaagact atacaaaatc gcttttccct cacgatggga cttgacgaca
    25081 tggttttctc ttaataaacg cagttgatgc gatacagcag attgttccat ttctactgct
    25141 tgggtgattg ctgtcacatt ttgttccccg tctttcagat acagcagaat tttgagacga
    25201 gtggggtcac tcaacatttt gaatagttgg ctgactttct ctacttttgt tttttgcgtg
    25261 tctggcaatc cattcacctc caacttgaaa aaagactgac ttctaaagtt taccactttt
    25321 tccatcagtc tcaagttttt ttattcttct gtcatttttt tgataatcgc ttcataagcg
    25381 gctacttttt cagctgcgtc ttctgaagag tttcctttta caccgatata aaatttgatt
    25441 ttcggctcag ttcctgaagg acggatagcg atccagccat tttcttccag cgtatacttc
    25501 aatacgtcag atggcggtgt cgttagtttt tctacgtttc cttcacggtc tgttctcgtc
    25561 aactctttga agtcttctgt ttggatgact tttgctccgg caaattctgt cggtgcttct
    25621 tcacggaatt tttccatcaa agcagagatt tttgcagagc cttcttgtcc gctcattgtc
    25681 acagaaatcg ttttttctgc gtagtagcca tatgtttcaa aaatcgtttg caagccgtca
    25741 tacaatgttt tgccttgttt tttatagtaa gctgctactt ctgctaataa gagcaatgct
    25801 tgaatcgcgt ctttgtcgcg aacgaaaggt ttaactaaat aaccgtaact ttcttcgaat
    25861 ccgaacataa aagtatggga atgatcttct tcaaattgtt gaattttttc agcaatgaat
    25921 ttgaatcctg tcaatacatt gaacatggta gccccatagt gttccgcaat tgctgtaggc
    25981 aattcgctag aaacgattga tttcaatacc gctgcatttt caggtaaagt gcctcgcgat
    26041 tggtgtgctt ccaaaatata ttgagcaaac agggctccga tttggtttcc tgtcaatact
    26101 tcataggagc catttggcaa acgaacggct gcgcctaaac ggtctgcatc aggatccgtt
    26161 gcaatcagta agtcagcgcc ttctttctcg ccaagtcgca tcgcatactc gaaagcagag
    26221 tgttcttctg gattcggtga tttgactgta gtgaaatccg ggtctgcgac agcttgttct
    26281 ggaactagta cgaattgttc gaatccagct tgtttcaacg cacgttcgcc caacattttt
    26341 cctgttccgt gaagtggtgt gtacacaagc ttcaaatctt tacccattgt ttccaccaat
    26401 tcatgatcaa tcgttactgc tttgatttct tctagataag cggcatccac ttcttctccg
    26461 atgatcgtga tcaatccgct atgttcagct tcgtcgtcag ataatacttc aacttttaac
    26521 ggattgctga cttcacggac atatttcgtc aatgcatctg cgtctgcagg aggcatttgt
    26581 cccccatctt ctccgtagac tttatatccg ttgtaagctg ctggattgtg ggatgcagtt
    26641 accatgattc ccgcaaacgt cttcaaatga cggactgcaa aagaaagctc tggtgttgga
    26701 cgtaaacttt cgaatacata agaagggata ttgtgatgtg ctaatgtttt ggctgcttcc
    26761 atggcaaatt ctggtgattg atggcgtgag tcataagcga tagcgacacc tcggcgtttt
    26821 gtttcttcac cttgtgtatc catgaaacgt gcaagtcctt ctgttgcttg tcttaccgta
    26881 tagatgttca ttcgattgat cccaggtcct aagattcccc gcatgcctgc agtaccgaat
    26941 tccaacggtg cataaaaagc atcttcaagt ttttcaggat cttcacccag atcatttaat
    27001 tgttttttta agttttcttc taaattttct tcatgtaccc attgttcata cacttgttcc
    27061 caagacatga aaataacacc tctttctgaa atatggttct taataagtat agcattcact
    27121 tattagatat gaaagtatga ggaccaaaaa acgcttaaat caagaaaaga ttcttttatc
    27181 aagcacggtt acaagtattc gcataaatcg gattcagtag atttcacgca aaaaaaagag
    27241 taaaagaaca attgaaaccg ttctttcact ctttttaaac ttatgcagtt gtttcttcta
    27301 aagattccaa gtatttatac acttgttgtc cagcaatccc accatcacca acagcagtag
    27361 tgatttgtcg caaatctttt tcacgaacat ctccgatggc aaaaacgcct ggtatattcg
    27421 tcttcatttc gttgtctgtt ggaatccagc cggcttcgtt cgtcaaccca gcttctctaa
    27481 atggtgcagt taatggatcc aatcctacat agatgaatac tccgtctgct gccaaatcaa
    27541 actcattgcc agttttgaca ttacgtgctt tcacacctgt cactaccatt tcatttccaa
    27601 cgatttcgtc aacgactgta tcccacataa aggaaatttt ttcattcgca aatgcacggt
    27661 cttggatgat cttttgtgca cgtaattcat ctctacggtg gacgatgact acttcagaag
    27721 caaattgtgt caaatagatg gcttcttcta ctgctgagtc tcctcctccg atcactacta
    27781 gacgtttgtt acggaaaaat gcaccatcac aaactgcaca gtaagaaacg ccacgtccag
    27841 caaattcttc ctcgccaggt acgcctaatt tacggtggat acagcctgtt gcgataatga
    27901 ctgtttttcc ttcgtaagtt ttatcttcac aaataacttc tttgtggctg ccgtgatcct
    27961 tgatatcttg cacgattcca taagcatttt ctgtcccaaa tcttgaaaca ccttcataca
    28021 tcttataagc tagatcagga cccatgattg aatcgaaacc aggatagttt tcgatttctg
    28081 ccgtattgtt catttggccg ccaggagcgc cttgttcgat catcaatacg gataagtttg
    28141 aacgtgaagc atataaagca gcagtcatcc cagcaggccc tgcgccaata acgattacat
    28201 catacatatg tagattctcc tttacttaat caagtaagca tactttacag ccaatgcgaa
    28261 aaaaagtcta ttgttctgct tgcgtttctc gaatagcttt attatactcg aaaaatccgc
    28321 tgaaaaacaa tagactttta taaatcattg atcaatcgtt cgaaagattt tctccatttg
    28381 aatcaattac ttttttatac cagttgaatg atttcttctt cgaacgggct aatgtgccat
    28441 ttccttcgtt gtcacgatcg acataaatga atccgtacct tttcttcatc tcacctgtac
    28501 cagctgatac taaatcaata catccccaag tcgtatagcc taatagatcg actccatcta
    28561 attctactgc atctttcatt gcctggatat gtgctgccag atagtcgatt cgataatcat
    28621 cttccacata accattctca tcgggcgtat cgactgcacc taagccgttt tctacaataa
    28681 atagtggttt ttgataacga tcataaatat cattcatcgt tattctcaag cctaatggat
    28741 caatttgcca accccattca cttgcttcca gatatggatt tttgacagaa gcaaagatat
    28801 ttcctgcagt ctgttccaag agttcgttgt cagtagtcga aactcgagaa gaataataag
    28861 aaaaggagat aaaatcaaca gtatgttctt tcagcaatgc ggcatcccca ggttccattt
    28921 tgatctgaat gccatttcgt tccatttctt tcaatgcata agcgggatat tctcctcgtg
    28981 actggacatc aatgaagaaa taattttctc gatctgactt tctagaagcc caaacatctt
    29041 caggattgca agtgtatgga taatatgctc ctgctgccag catacagcct actttgttct
    29101 ccggatctac ttcatgagcg atcttcgttg cgatggcact tgcaaccaac tcatgatgtg
    29161 ccgcttgata tttgatttgt tcttcatttt ctccttcttc aaaatacagc cctgcgccca
    29221 tgaatggtgc atgaaggatc atattgatct cattgaatgt caaccaatat ttgacaagtc
    29281 ctttatagcg attgaagatg actcggcaca gattttcata aaaaccaacg agttctctgg
    29341 aacgccacgc accatattct ttgatcaaat gcatcggaca atcaaaatgt gtaatcgtca
    29401 caagcggctc gatcccgtac ttttgacatt ctttaaagac atcctcataa aatttcaacc
    29461 cagattcatt tggctctttt tcatcgccca ttggaaaaat acgactccaa gcaatggaca
    29521 tccgataagt cttgaacccc atttctgcaa ataaagcaat atcttcctta taatgatgat
    29581 acatatcgat cgcaattttt gctggataaa aatgattttc atcaaattca aacattttct
    29641 tttttccagt aatgacagga aaacggtctt ctccgattgg cacaacatct acgttcgcta
    29701 acccacgtcc gccttcatca taaccgccct cgcattgatt ggctgctgta gctccgcccc
    29761 ataaaaaatc ttttctgaat cccatcatac ttcctccatt acatgtgtat tgcttgataa
    29821 ttctcttgcc agttcatctt ggacttcgat tccttctgct tttaaccggc tgattgtttc
    29881 cttgaattgc ggtaaatact gctgatgtgc aataaacaac tcatccatga tccgttttgc
    29941 tgtctttcct gatcgtataa gcggattgat cgtaaacgct tgcaaagctg ttccataatc
    30001 accagtcacg gctgcttcga tcgtcagcaa ttccatattc ttcattactt gcaaccagcc
    30061 tctttcagca gcaggaaggg aaccaaacgc tacatttcgt gcgccttgtg cgccaacata
    30121 ggcagtgact tctactacac aatctgccgg cagatcagga acagctccat cattctttgt
    30181 agataccacg atctgtgttt ctttatttcc atagatcgaa gcaatcgttt cacaggcagc
    30241 atctgaataa taagcaccac ctcgtttttg caattgttca ggtttatgat ccaattctgg
    30301 atctttgtat aattcaaaca gctctgcttc tgtttctttc acttgctgcg ctcttgtacc
    30361 aatcgtttga tattcttcta aagcatgttc cagcatttct tcttcacgat agtaatagcg
    30421 gtggtagcca caagggatca tgttcatttg ttttaattgt tctttgaaaa acgggatgtc
    30481 aaagatattt ttcggcaatc cattgtcttc atcgtataaa cgatcaatca attctgccgt
    30541 gcggtcattt cctttattat ctgctacttt gtgccaatgg aagtgattta gaccggcaaa
    30601 cttgtagatc agctcttctt ttttcttgcc aagcattgtt ggttctgtca tcatcgccat
    30661 tactggcaca ttgcataatc cgatcacttt atcccatttg ccatatctga taactgcttc
    30721 tgtcaccatc ccgcttgggt tcgcgaaatt aatcagccaa gcatttggac ataaacgctt
    30781 catgtcttca acgattgcca aaaccactgg aacggtacga aaagctttga atatcccgcc
    30841 agcaccattt gtttcttgcc caatcatccc ataataacta gggatccgct cgtctttgat
    30901 tcgtgcatct aattgaccga cacgaaactg agtcgtaaca aaatccgcat ctttcaatgc
    30961 ttcttcccga tccagagttg catagatttt gacttcatac ggagaagcat cccacatacg
    31021 ctgagacatc tgactgacga tctcaagctt ttcttttcct tcttctacat ctacgagcca
    31081 gatttctcgt atcggtaatt cctcatatct tttgataaat ccttccatca actcgggggt
    31141 gtaactgctg cctccaccaa tcgtagcgat tttgattcct tttgccatgt tctcactcct
    31201 ttacttttta cacttttatt atcttttttg taaacaaaac aaacaataga aattcgtttc
    31261 tttccagtaa catttacaaa agttagtgtt ttcttctgga tttttgtaaa tatcttgtca
    31321 aaaaatgtct ttattaagct atataacata aatccgttta caaatcattt tcaaaaaagt
    31381 tataatttac gtaaaaaaac aagaagaagg tatctacatg ttattacgtg aaaaaatcaa
    31441 agaaatctcc ttttctcctt cagaaaatga agtgattcag tttctcttaa gcgattctcc
    31501 agcacttggc gaaatgacca tccaagagat tgctgagcga tgttatgtcc acccgtccac
    31561 actcatccgt gtagcaaaaa aaatgggttt tgatggctgg gtcagttttc gtaatgcttt
    31621 tttagctgaa acagcctatc ttgaagggaa ttttcaagac gtagatgcga atcttccttt
    31681 ttccaccaca gacggtatta tgacgatcgc aaataaaatg gcttctttag aacagatgac
    31741 gattcaagat acactttctc tcattcatca tgaccaactg caaaaagcca aacaactaat
    31801 gcttcaagca aaaaaaatcg tgattttcgc cagtaactcg aatactttga tttctcaaga
    31861 tttcatgctg aaaatgaagc gcatcaaaat ggatgtcacg attgtcacca cttttggtga
    31921 atcgttctat gaagcgtaca attgcacaag tgatatgtgt gggattctga tttcttatac
    31981 gggtgaaaac aatgtgatcc ttcgtaccgc agaaatattg aaacaaaatc atgtgccaat
    32041 tttagctttg accagtatcg gtgattcaac gctatcttca atgagtacag ccgctttgcg
    32101 acttacgaca cgcgaacgac tgtattcgaa aattggtaat tttacgatca atacatcgat
    32161 ttgttatctt ctagacgtgt tatatagttg tgtttttgca gaaaattatc aacagaattt
    32221 agtccatcta atttctgtag gacaaaaatt tgataaacgc cctatcagct cagccatcat
    32281 gaaagagcct ttcaattttg aaaataaaga agtggactaa aaagaatagt taagcctagt
    32341 ccattctttc acctaaccgt tcaggtcagt gcggtgataa gtgcatcgtc tggcccgacc
    32401 gtttcccctt gattttccaa aatatccaaa taatccatgt aattgatgac aatcacaggt
    32461 gtttctaaac tgtatccttc ctcttcgatt ttctttttgt cgaagcgaag caacagatcg
    32521 ccttgtttca ctctttctcc ttgctgcacc aagctttcaa aatatttccc ttccaactga
    32581 acagtatcca atccgacatg aatcagcaat tccagacctg cttcagaaac catcccgatt
    32641 gcatgtttcg tcggaaacag ggacatgact gttccgtcaa aaggggcacg tacttctcct
    32701 ttttcagggt agatcaagat tcctcgacct agtgttcctt gtgcaaaagc tgcatccttt
    32761 gctgcattca gtggagcgat ttgtccttgt atcggtgttt ttacaatttc tttacctgat
    32821 cttttctgtc tatcatctgt ttcatctgct tcctccttcc aaaagaaaaa agtcagcgca
    32881 aaggatacca ccatcgcaat ggcgatccca atcaatacat taatcacatc tgttaaatca
    32941 ccatctggtc taatatagtt gggaattgta aagatgccta gtccaccttg attccaagaa
    33001 gttaaattca ttaacccact atacgcgccc cctaacgcac caccgacaca ggtaaaaata
    33061 aatatttttt tacgtggcaa tgtaaaccca taaatagctg gctctgtaat ccccacaata
    33121 ccagaaacaa tggctggtat tgcaattgct ttgtcttttg gatttctaag cttaaagtag
    33181 agtgcggtaa gtgctgcagt ttgtccaaaa gacactactg caacaggtgt cataatattt
    33241 tgccaaccat tcacagaaaa ttgcatcaat cccattggta cgattgccca atggagacca
    33301 aacatcacca gaatttgcca gaagaagcct acgattgctc cataaataac tggcgaaagt
    33361 gctaaaagtg tttcaaatcc agccattaaa atatcagttg cgaatgtgaa tactggtcca
    33421 acgagtaaaa aacctaaagg cattgtaata agaacggtga agaatggaac gaagaaattc
    33481 gcgatcatct caggtactaa ctttttaatt ggtttctgaa tttggctagc taacaacaca
    33541 ataaagataa tgggaatgac agaagaagcg tagttacgcg caatccaggg aatgccgaat
    33601 actgtttgat aaataggtgc ttcaaaaata gtgccagaaa atatggtcgt tagtggtgct
    33661 tctgctgcac cactcaaagc ttccagcgac aagctaggat tcattaatgc tgcgccaagc
    33721 aacatcccta cgaattcatt tactttaaat ttacgggcag ctgttaaacc gataataaca
    33781 ggcataaatt ggaatacaga atcaccgata ttatcaaaca taacaaatgt gccagaaccg
    33841 cggtcaaacg cgcctaagaa gacaaggaat gctgtcagtc cacgaatcat cccgtcagca
    33901 gctaggatag caaggaatgg ttggaaacag cccgatattc catcaataag catatcaaac
    33961 ggtctcattt tttctgttga aacagcctca tcaaaagtaa ggtttcctgc ttttataacc
    34021 tcttcaaaca cgactggcac atgattcccg atgacaactt gatactgccc tcccgctcgc
    34081 attaccgtta caataccgtc catatttttc aagatctcat catttgcttt ttcttcgttt
    34141 tttaatttga aacgaagcct tgtgatacaa tgtgtcaaac cattgatatt ttccttcccg
    34201 ccgacatttt ctacgatttt tctcgctaat tcttggtatt ttcccatttc tattccctcc
    34261 aatttattag tggaaggttt agccaactga atgtcactac ccttatttca tacgttcttg
    34321 ttaatttatc ctcctttttt tatttattta caacgattct catgcactaa acgcgcagta
    34381 tggatcgtta aatacatttt ttcgtccgtg gacatctggt agttgaacgt ctcggctaaa
    34441 aagtctgcta ttttctcaac gcaaccaaac gtatccttat attttttttg aataacctga
    34501 aacaattcct cagaaacttc tcccacttct gatttttctc catctaggac gcgtgtcaga
    34561 aaaaaacgta agtgtgtgac gaatcgaaaa aaatggactg aatcttcatc aaatgtaatc
    34621 ttatagtaat agcggacgat atttagaata tcctgtattg ttatagtgaa caggtaggta
    34681 ttgtcttgtt ggctttgaat gccttgtgca ttgactaaat gcaatgcgat aaatccggct
    34741 tcatcttctg ataacttgat gtggtattta tccgagattt tcttcactgt tttttttccc
    34801 gatggcatac tctttaggaa atagttgttt gatatcatac agcaatacat ttggtacaaa
    34861 tattcccatt ttgtgacgtt cgatagccgt atacaaatga tccgtcaatg caatatacac
    34921 agaatcattc aactggactt gtagagcctc ttttgtatat tcaatgattt cacaagaaat
    34981 ttctaagtac tcaagaggaa tcgcttgtgc caattcttgg aactgttgtg aaagcccgtc
    35041 atttgtcaac tgaaattttt tgtcgatgca tgagtcaaaa atcgggtcac ccactttctt
    35101 tttgaatgcc aaaccttttc ccatcacgat catttcctga tttctttcat ccctggttat
    35161 tacgacgttg ttattcagta ctttctcaat cagcatgttt tgctcctttc catgatatat
    35221 agggctgtat ccaaaacaaa aagaaaaacc caaaaagccg tccatggaac catgggagac
    35281 tcatcaggtt tagcttgcat caggcaatca ctatccaagt tgtatcgttt tctttatatt
    35341 atcgaaagca aacgcttttg tcaacgcttt cttagtggtt attagccgct tttgactagc
    35401 tgtcgtcgtc ttttttaaat cgggtgacta cgccactaac gctcacagat gaagaatctg
    35461 cgaagctctg ttttattttc actctagcgc ttagggcaaa atgctactat gctactgtca
    35521 caacctcttt ttacacacca ctgctttgca gtttgtacat atctgcatac aaaccatttt
    35581 gcgctagaag tgtttcatga ttcccacgct ctactatatg acctttgtct aaaactaaaa
    35641 tcaattctgc atcccgaatc gtggacagcc gatgggcaat cgctatcgtt gtccgtcctt
    35701 gtcgcatttt tgccaatcct tcttggatca atccttcggt ttcagtatcg atatttgctg
    35761 ttgcttcatc caatactagt attttaggat ctgtcacgat tgttcgcgca aaagtgatca
    35821 gctgtcgttg tccactagaa tagctggcac cgtgttcgat cacttttgag tgatatcctt
    35881 ttggtaattc atcgatgaac ttgtttgctt gaacaaactc agctgcatgg cgaatctgtt
    35941 cgtctgtgat agctggattc atcatccgga tatttcctgc aatatctcca taaaacaaga
    36001 aggcatcttg caagaccaat cccattttct tacgtaactc ttccattgga tattctcgaa
    36061 tgtcatgatc atcaatcaag atttggcctt cataaaattc gtaaaaccgc atcaatacgt
    36121 tgatgatcga gctttttccg cttccggtgt gccccactaa tgcaacggtc tcacccggat
    36181 tagcgacaaa agagatattg ttcaatacat tgtgctctcc atcataagaa aagcttacat
    36241 tgcgaaactc gatcttacct gaagtaatcg cttgatctgc tccaggattt tgttgcggtg
    36301 catattcttc tgtatccatg attttcaaaa tacgactacc ggcaaccatc ccatctgtaa
    36361 aggtgctcaa aaagtccatc atttgagaca ttggattaaa aaattcctgg acataggtcg
    36421 taaatgcata gatcagtcca acttctacgg gagaatgcaa cgcatcgatc ccaaacaaag
    36481 tcaacgccaa cgcaatcgcc aatgcataga gaaaattgat gattggactt aacagaatcg
    36541 aattcgtttt gatcatcgca tagcgcgtgt ctaaatactc gttgttcact tgttcaaatt
    36601 ctttagctaa acgagcttct tggcgaaact gctggataat acgcatgccg gaaatcgatt
    36661 cattcaattt cgtgttcagc tgacttaatt tttcccgcat atttcggtaa atacgagaac
    36721 tgaatttctg gtaataccag atgacgattc ctaaaatcgg caagaacacc aaagtccaca
    36781 aggcaatttc tccattgatt tgaaacatgg caacgaaaga agaaacgact gcaaagatcc
    36841 cagtcaatac catcaaaaag acataccaaa attcaaatag cgtttctgtg tcattcgtca
    36901 ctcgagaaac ggtcgatcct gccggtgtct ggtcaaaata acgcatacct agtgtatgca
    36961 gcttttcaaa taatcttacc cgaacatact gataggtctt caatgaagcc atggaataca
    37021 aataccattg gaaaaaccaa atgatacttt tgactatgac tccaaacaag tacacaccag
    37081 caaaaaacaa aataacttgt gtcgtagctg actgtttagc cagatgatca tccataaaag
    37141 tttggatgat ccttggcaat aggacattga tcacagacag ggcaaacgca aataagatag
    37201 caacgatgaa cgttcttcta aaaggtttgg cgaacttcat cagtcgtttg atgatcttct
    37261 tctgttcttt aagtggaatc gattttgtcc attccgattg ataattttca tccatcaggc
    37321 ctcgcctcct tctatcttcg cttctaattg ctgtttttcc cacattcgtt cataccagcc
    37381 acctaatgca agcagctcgt ggtgggttcc tcgttcaatg attttccctt gatcgacaac
    37441 aatgatttct tttgcatgca tcacgctgct taaacggtga gctgcaatga tggttgtttt
    37501 ctcttttctt atttctttta aattatgcaa gatagcttct tctgttttgg catctactgc
    37561 agaaagtgca tcatctaaga tcagcaattc aggatttttg atcaatgctc gagcaatcga
    37621 aatcctctgt ttctgtccgc cagataaaga gactccacgt tctccaacta aggtatcata
    37681 accttttggc aattccttga tttcattgtt gataaatgct aaagaagccg catgctctac
    37741 ttcttcttct gaaagttttg gattagaaaa acggatatta tctcgaatgg tcatcgaaaa
    37801 caggaagtga tcctgaggca cataaccgat tgatcctaat aaagcattca atgaatattc
    37861 tttgatcgaa cgtccgccaa attggatttt cccttgataa tcatcgtatt cacgcatcag
    37921 taatttgatg attgtcgttt ttcccgcacc agttttccca acaataccta atgtttctcc
    37981 ttcttggatc gcaaatttga tttgttctaa tgctggtgat tcttctttcg gatagttaaa
    38041 agaggaaata gctacattca gctcgccttt tgccggggtc tgcactgcac ccagttgttc
    38101 cacgatatgc gtcttttcat gcaaaagttc attgacgcga tcataagaag catttcctct
    38161 ttccaatacg ttgaacaatc gaccaatcgc aaacatcggc caaacaagca taccgatata
    38221 gctaataaat gagaccatct gcccgattga gatcgtgcct tccaagacat actttccacc
    38281 taaaataatt gttgcaacat aagacagacc gataatcaag gtaatgaatg gatcaaataa
    38341 ggagtctaaa aaattgactc gtttgttttt aataattgca tcttcaatct tttcttcaaa
    38401 atccgcgaga tcttcttttt cttgtccaaa tgttttaatg actttgatcc cagtgatact
    38461 ttcttgtgtc ttatcattga tactagaaaa agcttcttgc gaatcacgaa aagcatcgtg
    38521 gagttttgac cccagcactc gtgaagtaac ggctagcaaa ggcagaggaa tcagcgcaat
    38581 cagtgttaag cgccaatcaa taaacaaaat catcgcaatg atcgttgttc cgcccgtaat
    38641 cactgaatct gcaaaggtca agattccagc tcctgctaca ttctggattg cattcaaatc
    38701 attcgttgca tgagccatca aatctccagt ccgatatttt tggtaaaaga gattgtccat
    38761 ttctgtaaaa tgtttgaata gctgctgccg aagttctctc tccaaccgag ctgcacttcc
    38821 ccaaatattc gtccgccaga tataacgaaa aagatattgt aagaaagctg caaggatcaa
    38881 taataagatc caccaggaaa tgatctttat tgataaattc ttatcggcga tctcatcaat
    38941 gatgatccca atcacttttg gaggaactaa ctgtaccaag gccactgcga atagggagaa
    39001 aatcccaatt aaataatgtt tcttttcctg acgaaaaaac caacctaatt ttctaaaaat
    39061 cgacatttta ctcctccttt gtctttatca agagcatagg attgtctgtg cttgtctttt
    39121 ttgctgaata gtctgaatag tctaacaaat tacgaaataa aaaacggact gaaaaaatca
    39181 gcccgcaaaa agtctttggg tcagaaccta ttttttccct tggttcttca tttgctgcat
    39241 catctggcga attttcttct cagatggttt ttgccccatt gacatcatca ttgatcgcaa
    39301 catgtcctca ttaacgggag gatttttttc taaatagtct ttcatatatt tacgcgctag
    39361 gaaaaagcca cctactgcgc cgattagcaa agcaataata gcaataagta cgacaatacc
    39421 tgttgatacc atataaatct ctcctttcta tttccgaatt gctctcaatg atttatttta
    39481 ctaaagttta ttgaaaaaag aaaggcgtat tctttagttt catgcaaaaa atagtggttt
    39541 ttttagagaa gaacaaaatt tctttcatta accagcaaga cacaccacga aaaaaaatta
    39601 ggcataccga atctttttct ttaatttttt ctttctgagg tgtagaatgt aatcgtagat
    39661 aaataggaaa ggtggttttt tcttgaataa tcaatccaac aaacaatttc ataagcatcg
    39721 acttattgaa catacaaatg gtttatcttt agctgaagta aatggaacag tggatgtacc
    39781 aaagggaaaa agttttttta aaacacttct tgcttactct ggaccaggtg cattagtagc
    39841 agttggttat atggatccag gtaactggtc cacttcgatc actggtggac aaaatttcca
    39901 atatttattg atgtcagtta ttttgatgtc tagtctaatt gctatgcttt tacagtatat
    39961 ggcagcaaaa ttaggaatcg tgacccaaat ggatttggcc caagcaatcc gagcacggac
    40021 aagtaagaca ttgggtgtca ttttatggat cttgactgaa ttagccatta tggcaacaga
    40081 catcgcagaa gtcattggtg cagcaatcgc cctctactta ctttttgata ttccattggt
    40141 gattgccgtc tttattactg tattagatgt tttcgttctc ttactactga caaaagtcgg
    40201 gtttcgtaag atcgaagcac tggttgtctg cttaattttc gtcattttat tcgtttttgt
    40261 ctatcaagtt ggcttgtcta acccagattg gcacagttta tttgctggct ttatccctaa
    40321 cagtcataca ttctcagact caccggtcat cggcggcgaa actccgctga ctggagcttt
    40381 agggattatt ggtgcaacag tcatgccgca taacttatat cttcattcag cagtttcaca
    40441 aactcggaag atcgatcacc aaaacgaaga agatgtagca caagctgtac gtttttctac
    40501 ttgggattct aatatccagt tgtcaatggc ttttctagtc aatgcactgc tattgatcat
    40561 gggtgtcgct gtcttcaaaa ccggttctgt ccaagatcct tccttttttg gtctgtatga
    40621 agctttatct gatccatcag ctatgagcaa tggtattttg attgctgtag caaaatctgg
    40681 tatcttgtcc actttatttg ctgtagcact tcttgcttct ggccagaact ctactattac
    40741 tggtacgttg accggtcaag tcatcatgga aggattcatc catatgcgta tcccgatttg
    40801 gctaagaaga ctggtcacac gattgctttc tgtgattcct gtattgattt gtgttttgtt
    40861 tacaagtaca aaaggaacga tcgaagaaca tgccgctttg aataacttaa tgaatgaatc
    40921 ccaggtgttc ttggcttttg cgttgccatt ttcgatgatt cctttactga tgatgaccaa
    40981 cagccaagcg gagatgggta gccgatttaa aaattcattc atcgtaaaac ttcttggctg
    41041 gttctctgtg attagtctaa cttacttgaa cttacgagga ttaccaggac agatcgaaag
    41101 tttcttcggc gagcaagcca cttccgctca actagcattg gctgatcaca tcgcttatgt
    41161 aatcattgtt cttgtccttc tcctgttggc atggacgatt ggtgatttat ataaaggaaa
    41221 caaaaaatat gaacagcttt tgcaggaaac ggtagaagaa atcgaataaa aactgatttt
    41281 tccacacaaa gacccttgat agttaggtta aaaatctagc taccaagggt ctttttattt
    41341 agaagataaa gcttttttca gatttaattt actgaaaact agcatgcttg ttactacgat
    41401 gaattgcgtt aatggcacgc ctgcaccagt gtagacactt aaaaaatagt cctttttttc
    41461 tggcgcataa gaacaaaacc atgagacacc gaaaagaaaa accaattgtg tcagtattaa
    41521 tccggtgacc acaatcaaag aggaactata ataggcacga tcagcttttg caaatacggt
    41581 tcctagtatc ataagtaata tccctgctat tgaagcaatc agcacgggaa tagccagaac
    41641 ttggtaaatt tttatgatga cattcgcgac ttttgctgat tttgttaatt ggaaattagg
    41701 tgttctcggt tcattactat cgatccaatt ctccattagc ggaaggttta ataattcttg
    41761 agcctgtttg acttgttctt gcgttccgaa actataattt cctccttgat ggaaatcttt
    41821 atatcgccat gcataagcgt atcctgattg aaggaaatcg aatacgattg gcagatcact
    41881 catcgtcttt ccatctcctg atcccatcag atagaactct tttttctttt tgatggttcc
    41941 cttgtgatag gcttcttcta attcttggtt gacttttttc caaaaatcat tggtttcttt
    42001 tgcattgttt tggtaataac cagattcgct taccacatct cgcaatgccc aaaagatgat
    42061 atctccgggt aattctttta cttttccacc ttgagtccac gcgtgagtcg tatataattc
    42121 gtcaatacgg gtagaaacag tatggaatgt aggagatgcg gctaatgctt tatttagtgc
    42181 agcatgtgag acccatatct cactgttttc tttattcaag ttcgtttcat catctattcg
    42241 gatcagttgt ttcatcaaac gtccaaattc tccactggaa cggtcattga tggttgcaat
    42301 accgtatgtt gtctcatttt tatattcgat ccactgatag acgatggaaa agccaactat
    42361 aggaagtaaa aacaatgaaa gtttcagcca ttcccgtttc gagagaagaa agagtcgttt
    42421 ttttaacttc tttacttctt tgaaagaaaa gtcttgccct agcaagatct ggacgaaact
    42481 gatacccaaa gctactacag taaaaggaag aagccagata gaatcttcgc gaatgaacca
    42541 aaagaaagga aaagcgacag aaagccctaa aaaccatggc agcaattttc tgctagaacc
    42601 gtatttgcgg taataaatag ctaataaaca agcgataatg atctcaacag ccggaatcac
    42661 gagggcatta cgatatgttc gagtggaata ttcatgtgta aaactaatcg gagaaaacaa
    42721 aaaataaaaa tagatactta aaagaagata cttgttcttc acaatcggtt taaacgcgag
    42781 tgcaaccaat atactggcaa aaatactgaa catgccgaac aaaactgcat aagaaagatg
    42841 cagtttatgt gcaagtacta aaaataagct gtaggagatt cctttggaaa gtgttgtagt
    42901 cgtatactcc cctagccatt taccatccaa aatattttga gcatagtgaa tcagcagctg
    42961 atcatcatag gtcgccgaca tatctgttcc atagattgct ttactatata aatacaaccg
    43021 aataattgta aataatacgg ctatgccaag aataaagtat gtatgtttct tttttagttg
    43081 gtcgatcaag gggtttaaaa tgttcagcat cttttcgtcc tttcaatcac gttttgctac
    43141 ttgtttgtga aacttttcgt tttttacttt tctatcagta taccctgttt gtctgaattt
    43201 aaaaagaggg ggcaaaagga gcatggcgaa aacgaatgca aaaagcactc ctttgtttct
    43261 taaacaaaaa aggacaaggc agactgccca atcctttttt gttgctgtca ggtagaaaac
    43321 ttaaagtgtg acttcttctg ctaataactc agggaacact tcgggatcga atgcttgcgc
    43381 tttgaacatg gaaatttctt ttccatatgg tgcgtaagct ttctttttat ctttaccgac
    43441 atatggggtt tctaatattt tcgaaatgtt gtcaaacatt ggatcatgga cgattttgtt
    43501 caatgtgtca aatccaattg ttccaaaacc gatattggca tgccgatctt tatgagaacc
    43561 ttgtggattt ttagaatcat tgatatgcag tactttaagc cgatctaatc cgatgacctt
    43621 atcaaactct tccattacac ctgaaaaatc ttcttttata ttatatccgg catcattcgt
    43681 atgacatgta tcaaaagtaa cggataattt ttcattcaat tttactccat cgatgatttg
    43741 cgctaattcc tcaaatgttc gaccgatttc cgtacctttc cctgccatcg tctccaaagc
    43801 gatctgtgcc gtctgttctt ttgtcaaaac ttcattcaac ccctcaataa ttctagcaat
    43861 cccagcttct gcccctgctc ctacatgagc gcctgggtga agggtgattt gagtagcacc
    43921 taaagcttcc gcacggacaa tctcttctct cagaaattgt acagcaaatg gaaacatttc
    43981 tggtttgatc gtatttccca aattaatgat ataaggcgca tgaacgacga tatttgttag
    44041 tccgttttct gccataaatg cttttccggc atcaatattc atctcttcga tagcttttct
    44101 tctagtattt tgaggagcgc ctgtatagat cataaaggtc gaagatccgt aactggctgc
    44161 ttcttctgcc gcgcctaaca gcattttctt tccgctcatg ctgacatgtg agcctaataa
    44221 catggatgga ttcctccttg aaaggcaatt gtttcgaaca attcctgtat attgataaga
    44281 tgatgtacaa agaaaatggt ttgtttttct ttgccctgtt ctccatagta actgaaactt
    44341 tccctaaaga aaagcttaag aactctttca taaaaaagat catttcctca cgttttatct
    44401 cattcgaaaa agaaattcct gtcaaaaaca gccttgtctg tttttttgtc ctattatttg
    44461 ctataattag cgaaataaaa ccattagttg aggtgaccct tttgccacgc aaaaatactc
    44521 gaaaaaaaca gaatagaaga aaaaaagaaa aatggtttgt tcctaagatt atttttagag
    44581 tgtttcaatc attgactgtt tttattactg ttcttctttt gttgttcgca gctctaggta
    44641 tcggaatagg tgctggctac tttgcctatc ttgtcgaaga cacacaattg ccgactaaaa
    44701 aagaactgca gacagaacta ggaaacatta cggaaacgtc aaagattgtt tatgcagata
    44761 atacagagat atcaaaaatc cagacagatc tgatgcggac gactatttca tctgataaga
    44821 tttcgccttt attgaaaacc gcgattatca gtacagaaga tgaatacttc gataaacatc
    44881 aaggttatgt ccctaaagct gttttacgag cactagtttc agaagcgact ggcatcggct
    44941 cgtctggagg atccactttg acacagcaat tagtcaaaca acagatccta accgatgaaa
    45001 caacattcaa acgtaaagca aacgagatcg ttctttctgc acaagtagaa aaacactttt
    45061 caaaaaatga gatcattact acgtatttga atgtctcacc atttggtcgg aataataagg
    45121 ggcaaaatat tgctggagtc caggaagctg ctagaggaat ctttggtgta gatgcaaagg
    45181 atgtgactct gccacaagct gcttatatcg ctggtttgcc tcaaagccct atcacgtata
    45241 gtccatacac gaatacaggc gcgctaaaag aagatctttc tgccggtctt gccagaaaag
    45301 attttgtttt attcagtatg taccgtgaag gacaaattac gaaagaacaa tacgaagaag
    45361 cgaaagccta tgatcttaca aaagactttc tcccacaaca aatcgcggaa cagactgaca
    45421 gagaattctt gtattatacc gtgatgaacg aagcaacaag gattattgca caacaattgg
    45481 cagaaaaaga taatgctgat atgtcagatt catcggtcag tgaagcttat tatcaaaaag
    45541 cacagcaaac gatccaaaac aaaggatata cgatccattc gacgatcgat aaagatatct
    45601 atgctgcatt acaaaccggc gtagaaaatt atggctacat gttagatgat ggtgctggta
    45661 gccaagtgga gactggaaat gtattgatgg ataacagaac cggacgtatc tatggcttcg
    45721 tgggtggtcg gaattacagc cagaatcaaa ataaccacgc ctttgataca gaacgtcaag
    45781 ctggttcatc catcaaaccg gttcttgttt acggtcctgc aatcgatatg ggacttgtcg
    45841 gatcagaatc ccgtgtatca gattatgcaa cgacttggca agaaggaaaa aatgctggtg
    45901 agaaaatcgt caatgcgacc aatgaagggt cgaacacctt ccaaaccgtc cgtgagtcac
    45961 tggaatggtc caacaatatc ccagcttatc acttatatca agatgtgttg aattccggtg
    46021 gatcgaaaca atacgcttac gagcactact tggcgaagat gaactaccca gcaaatgata
    46081 attggggcgt tgaatcggct cctttaggga cagtagatgt aacgacgctt caacagacaa
    46141 atggattcca ggccttagca aatggtggag tctacgaaga aggctatatc attgattcca
    46201 tcacagataa tgcaggtaat gtgatttata aacatgaaag taattctgtc cgtatttatt
    46261 ccgaagcaac ggcatcgatc atgaatgaca tgatgcgtag tgtgatcaat gaaaaaatca
    46321 ctacaccatt caaagatgtc atctcaagct taaacggaaa tctaggaaat gcagattggg
    46381 tcggaaaaac tggttctacc aatgaatacc gagattcatg gctgatcgtc tcaactccat
    46441 ctatcactat cagtagttgg gcaggacacg atgacaatac tggaatggac agccaagcaa
    46501 gaatacgttc ggctaattat cttgccaatc taatcaatca agcttaccaa gcaaaccctg
    46561 agattttcgg cactgatgag aaatttgact tatcatccga tgtgatgaag aaaaaagtat
    46621 ctgggttcac aggacaaaca gctggaaaag ttactgtcaa taaaaaatcc atccaaacgc
    46681 caggaaaaac agtggagtca ctttgggcaa aaaatggtcc gaagaaaagc gcctttaaat
    46741 tcggcgtagg cggtactgat gaaaactata cagattactg gaacaccgct tcagcttatg
    46801 ctagagcgaa tccagagacc aaagaatcag acgataaaga tgattgatct aatagattaa
    46861 tcagaattaa agggagctgc gacaaaaatc ttgtcacagc tccctttttc cgaataaatg
    46921 gtgttcaaaa gcttacatct acgatattaa gacaaattta tgtaaaaatg tgaaaaac
//
LOCUS       DACEUF010000009        46896 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.010, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000009 DACEUF010000000
VERSION     DACEUF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.010"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            212..1018
                     /gene="proB"
                     /locus_tag="IXG20_000537"
     CDS             212..1018
                     /gene="proB"
                     /locus_tag="IXG20_000537"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ1608753.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1015..2262
                     /locus_tag="IXG20_000538"
     CDS             1015..2262
                     /locus_tag="IXG20_000538"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ1608754.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2338..2682
                     /locus_tag="IXG20_000539"
     CDS             2338..2682
                     /locus_tag="IXG20_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608755.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2799..3125)
                     /locus_tag="IXG20_000540"
     CDS             complement(2799..3125)
                     /locus_tag="IXG20_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608756.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3498..4700)
                     /locus_tag="IXG20_000541"
     CDS             complement(3498..4700)
                     /locus_tag="IXG20_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1608757.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4817..5332
                     /locus_tag="IXG20_000542"
     CDS             4817..5332
                     /locus_tag="IXG20_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1608758.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5531..5977)
                     /locus_tag="IXG20_000543"
     CDS             complement(5531..5977)
                     /locus_tag="IXG20_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1608759.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6024..7502)
                     /gene="msr"
                     /locus_tag="IXG20_000544"
     CDS             complement(6024..7502)
                     /gene="msr"
                     /locus_tag="IXG20_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1608760.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7856..8485)
                     /locus_tag="IXG20_000545"
     CDS             complement(7856..8485)
                     /locus_tag="IXG20_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1608761.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8469..8933)
                     /locus_tag="IXG20_000546"
     CDS             complement(8469..8933)
                     /locus_tag="IXG20_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1608762.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9420..10688)
                     /locus_tag="IXG20_000547"
     CDS             complement(9420..10688)
                     /locus_tag="IXG20_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1608763.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10919..11656)
                     /locus_tag="IXG20_000548"
     CDS             complement(10919..11656)
                     /locus_tag="IXG20_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1608764.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11723..12292)
                     /locus_tag="IXG20_000549"
     CDS             complement(11723..12292)
                     /locus_tag="IXG20_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1608765.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12420..13292
                     /locus_tag="IXG20_000550"
     CDS             12420..13292
                     /locus_tag="IXG20_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1608766.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13634..14506
                     /locus_tag="IXG20_000551"
     CDS             13634..14506
                     /locus_tag="IXG20_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1608767.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14503..16461
                     /locus_tag="IXG20_000552"
     CDS             14503..16461
                     /locus_tag="IXG20_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1608768.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16712..17563)
                     /locus_tag="IXG20_000553"
     CDS             complement(16712..17563)
                     /locus_tag="IXG20_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1608769.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17686..18153
                     /locus_tag="IXG20_000554"
     CDS             17686..18153
                     /locus_tag="IXG20_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1608770.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18416..18898)
                     /locus_tag="IXG20_000555"
     CDS             complement(18416..18898)
                     /locus_tag="IXG20_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1608771.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18998..19861)
                     /locus_tag="IXG20_000556"
     CDS             complement(18998..19861)
                     /locus_tag="IXG20_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1608772.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20459..21307
                     /locus_tag="IXG20_000557"
     CDS             20459..21307
                     /locus_tag="IXG20_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608773.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(21478..21819)
                     /locus_tag="IXG20_000558"
     CDS             complement(21478..21819)
                     /locus_tag="IXG20_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ1608774.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            21905..22612
                     /locus_tag="IXG20_000559"
     CDS             21905..22612
                     /locus_tag="IXG20_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1608775.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            22599..22922
                     /locus_tag="IXG20_000560"
     CDS             22599..22922
                     /locus_tag="IXG20_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1608776.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(23011..24048)
                     /locus_tag="IXG20_000561"
     CDS             complement(23011..24048)
                     /locus_tag="IXG20_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1608777.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(24495..25478)
                     /locus_tag="IXG20_000562"
     CDS             complement(24495..25478)
                     /locus_tag="IXG20_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ1608778.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(25894..26664)
                     /locus_tag="IXG20_000563"
     CDS             complement(25894..26664)
                     /locus_tag="IXG20_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1608779.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(26657..27592)
                     /locus_tag="IXG20_000564"
     CDS             complement(26657..27592)
                     /locus_tag="IXG20_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1608780.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(28186..29835)
                     /locus_tag="IXG20_000565"
     CDS             complement(28186..29835)
                     /locus_tag="IXG20_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1608781.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(30030..30926)
                     /locus_tag="IXG20_000566"
     CDS             complement(30030..30926)
                     /locus_tag="IXG20_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1608782.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(30919..31599)
                     /locus_tag="IXG20_000567"
     CDS             complement(30919..31599)
                     /locus_tag="IXG20_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1608783.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(31600..32382)
                     /locus_tag="IXG20_000568"
     CDS             complement(31600..32382)
                     /locus_tag="IXG20_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1608784.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(32375..33292)
                     /locus_tag="IXG20_000569"
     CDS             complement(32375..33292)
                     /locus_tag="IXG20_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1608785.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(33295..33579)
                     /locus_tag="IXG20_000570"
     CDS             complement(33295..33579)
                     /locus_tag="IXG20_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1608786.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            34367..37594
                     /gene="fss3"
                     /locus_tag="IXG20_000571"
     CDS             34367..37594
                     /gene="fss3"
                     /locus_tag="IXG20_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1608787.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            37707..38021
                     /locus_tag="IXG20_000572"
     CDS             37707..38021
                     /locus_tag="IXG20_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1608788.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            38034..38408
                     /locus_tag="IXG20_000573"
     CDS             38034..38408
                     /locus_tag="IXG20_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1608789.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            38418..38753
                     /locus_tag="IXG20_000574"
     CDS             38418..38753
                     /locus_tag="IXG20_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ1608790.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            38836..39981
                     /locus_tag="IXG20_000575"
     CDS             38836..39981
                     /locus_tag="IXG20_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1608791.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQCATCF"
     gene            40696..42555
                     /gene="ltrA"
                     /locus_tag="IXG20_000576"
     CDS             40696..42555
                     /gene="ltrA"
                     /locus_tag="IXG20_000576"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1608792.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
     gene            42649..42888
                     /locus_tag="IXG20_000577"
     CDS             42649..42888
                     /locus_tag="IXG20_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ1608793.1"
                     /translation="MTSKDYLSLFNFRVALGRMSELGYSMMFGEVEKNFFMKHIKGRG
                     YVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            42966..43640
                     /locus_tag="IXG20_000578"
     CDS             42966..43640
                     /locus_tag="IXG20_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1608794.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            43873..44307
                     /gene="dcm"
                     /locus_tag="IXG20_000579"
     CDS             43873..44307
                     /gene="dcm"
                     /locus_tag="IXG20_000579"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1608795.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            44308..45015
                     /locus_tag="IXG20_000580"
     CDS             44308..45015
                     /locus_tag="IXG20_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1608796.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            45005..45295
                     /locus_tag="IXG20_000581"
     CDS             45005..45295
                     /locus_tag="IXG20_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608797.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            45554..46738
                     /locus_tag="IXG20_000582"
     CDS             45554..46738
                     /locus_tag="IXG20_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1608798.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            46753..46872
                     /locus_tag="IXG20_000583"
     CDS             46753..46872
                     /locus_tag="IXG20_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1608799.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT        14569 a         9268 c         8883 g        14176 t
ORIGIN      
        1 aaactactcc tattctaaca tatccgaggc tgtaaaagac tttctttaca aattcttcaa
       61 gaaattctgc tatcttaaag taaaccatta attcgttctt tttccaaacg actgcaagtt
      121 gtacaaaaaa actatttttt acatgacttt actcgtcaaa acaagtagaa tagaacgaaa
      181 ttaatgagtg gatatttgat tgaggtgtca catgcgtaaa gaaattaaaa atgcaaaacg
      241 aattattgtc aaagtaggaa caagtacatt gatttatccc aatggaaata tcaatcttgc
      301 ggcaatcgat cagttagctt ttgtcctttc tgatcttaga aatcggggaa aggaagtgat
      361 cttagtctca tcaggagctg taggcgttgg tatgcataaa ttacatttgt cagaacgtcc
      421 gactttgatt cctgaacagc aagctgcggc ggctgttggt caagccgaac tgatgaatat
      481 ctataatcaa agattcagtg tatataatca acagacggca cagatcctcg tgacaagaga
      541 cgtggtagat tatcctgaaa gtcgtaaaaa tgtgaggaat acattggaac agcttttgat
      601 tatggacacg atccctatcg tgaacgaaaa cgatactgta gcagtggatg aattagatca
      661 tctgacaaaa tttggcgata atgatcagct gtcagccatc atggcaaaac ttgtcgcggc
      721 agaccttttg atcgtccttt ctgatatcga cgggttttat tcagataatc cgactacaaa
      781 tcccgaggct cagatgtatc gctatatcga taaaatcgat gaaaagattc tggcacaagc
      841 aggtggtgct ggtagcacat ttggaacagg cggtatgcag agtaaattaa aagccgcttc
      901 taggatattt gaagcaaaca gcgcaatgat tttagcaaat ggaaaaaatc cggcaattat
      961 ttttgatatt cttgcaggaa aagaagtagg tactttattt aaggaggact tgaaatgact
     1021 gacttgatgc aattaggaca acaggcaaaa gatagcgcgc aacaactagc acttatgcct
     1081 acaaaccgca aaaatgagct acttgttcaa atggcacaaa ccatcaaaaa gaatcaacaa
     1141 gcgatcattg aagcaaatca taaagactta gaaaaagcag tcgaaaacaa tatcagtgaa
     1201 acgatgcaag accgcttgca gctgacacct gagcgaatca atgccatggc tacagagatc
     1261 gaaaaaattg cttcgttaga cgatcctatt ggaaaagtag atgagatgtg gacgaacacc
     1321 gacggattga gaatcggcaa aaagcgcgtg ccactaggcg tgaccggcat catttacgaa
     1381 tctcgtccaa atgtcacgac agatgcagct agtctagcat tcaaatcagg gaatgccgtt
     1441 atccttcgcg gtggcaaaga agcattcttc tcgaatcaat tattagtcca attattgcag
     1501 caagtactgg tcagtgctgg cgaatctcct tatgccatcc agtttgtcga tgatacttct
     1561 catgaaactg caggacagtt gatgcgattg acagaatact tagatgtgct gattcctcga
     1621 ggcggagcaa aattaatcca acgagtcaaa gaacaggcaa cggtacctat cattgaaaca
     1681 ggtacaggaa ataatcacgt atatatcgat aaagaagcac agttatctat ggcagtgaac
     1741 atcatcgtca atgcaaaagc ttcccgtcca tctgtctgta acgcggcaga aacattgctg
     1801 atccattctg agatcgcacc ttttttcttg ccagctattg aaaaagaact ggtagaacac
     1861 ggggtctctt tacgtgcgga tgctcgtgcg ttagaatatc tagagacggc tgctttagca
     1921 gaagacacag attgggatac tgaatatcta gattatatcc ttgctgtcaa agtggtcgat
     1981 tctttggatg aagcaattgc acatatcaat cggcataata ctaaacattc agaaacgatc
     2041 gtgacagata gttatgcggc tagtcaacgt ttcttaaatg aagtggacgc ggcggctgtc
     2101 tatgtcaatg cctccactcg ttttacagac ggatcggtat ttggttttgg tgcagagatt
     2161 ggtatctcta ctcaaaagtt gcatgcccgt ggtccaatgg ggttaaatga attaacttcc
     2221 acaaaatata tcatttatgg cgatggacaa atccgcgaat aatgtttcgt gtgaaacatt
     2281 attcccgcct tttgcctttt ttctgtaaag gttctatcca actagaaagg tgtcaatatg
     2341 tttgaaattt taatgatttt ttttatttac cttatttctc tgaatatcgc tgcttttctg
     2401 ggagtcggta ttttatccct tttctttcaa ttcaaaaaac gaagcatagg gagtcaacgg
     2461 gaaaaatggt cgcagtattt tgacaaaatc gggcaaaaag ggctggttac acgcttgcac
     2521 attagttaca tggttgcatt atgcttatta gcgacaatca attactattc ttttttcgat
     2581 cattcaatcg cttatacgat tacgctactg attgctggga tttttcactt atcttataaa
     2641 tatcagctga ataaaaacca cttgaatcgg actttccgct aaaaaatatc cgtacgataa
     2701 gtagagtgcc ttgaagatcg gcaaaaaact gccggacttt ttagaggctg tgacaaagat
     2761 tttgtcacag cctctttttc catataaacg gcgttcgcct accagctgcc tggtaaatct
     2821 gtttcctctt cgttaagaag cgaaaatccg tcaaatgttt cgccatcata ataacgaagc
     2881 agctcatcca tggacaagtt catcgttttt tctttttctt cttccgctga ttcagccagt
     2941 agctgttccc gatacacctg atacatacca gctatttttt gctgcatcgc ttttctagtt
     3001 tcaccttccg cattgattat tggcaattct ttgatatagc cacgataaat caaccgatca
     3061 tctgaaaatt caatgggtaa ttcaacgagt gtcaacccga cttcctgaaa atattcttcc
     3121 agcatctatt atccccccat tctttttctt aaatgaattt tagcttacct actgaaaatt
     3181 ttccagtttc attattttga gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct
     3241 ttttccgaat aaatggtgtt cttctgccaa ctcctcaatc ataagtcaca atattcaaca
     3301 aacatgagaa actgtttttt gaaattgttc cttgtgattc agagttaaac gacagtctca
     3361 caacctcttg gtaaacggcg ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg
     3421 aaaaatatga gaagctattt ttgtttggct gttcttatga cttgatgcag gacagctttt
     3481 tcacaacctc tgatttctta ttcttcttca tttgtagcaa aataattacg gtgataaatg
     3541 atacaacata cactgataag aacaatggca cctgcaaaat aaaacgcata gctttcacca
     3601 atatgttcac caatcacacc tgcaataaat ggtgctaaag ctccaccgaa aaagcggaca
     3661 aaactatagc tggacgaagc aacgtttcgt tgcagacctg gtatttccat agcaatcgtc
     3721 gtcaacagtg tattgataat tccttggaaa aatcctgcta acacaatgct gatcggtaca
     3781 aggactggca tagaagttcc agctcctaat aacagcaaac aaataatgaa taagacaaat
     3841 gctgctaaaa ttgtccgata agtcgtaaac ttccgctcaa gagccggtgc aacgaaaatc
     3901 gaagcaatag ccagaagcac accccagcca aaaaatacaa aaccgacttc taattcagaa
     3961 tagccaatca ataaaaatgg cgcatacgca agtaaagtaa agaatccgaa attatataac
     4021 aatgcgatta gtccaactga acgaagttta tgattgctta acgctttgac accttcccaa
     4081 aggcctactt ttctctttgg tttctcagtt tcaggtaaaa gtaccgctag ggctataaaa
     4141 ccgcaaaaca tcaaaaatgc taccccaaaa aatggataac gccatgaacc actgcccaag
     4201 accccaccga ctagagggcc aacagccatt ccgcagccaa gtgctgcttc atacatcatg
     4261 attgcttttt ctgtgttacc aactaaaata ctgacgatcg ttgctaaagc agtagagaca
     4321 aataaagcat tcccgacacc ccagccggca cgtaacccaa tcagcattcc gatattccca
     4381 gataatccgc caagaccagc gaatacaacg atgataaaca atcctgtcat caatgttttt
     4441 ttcaaaccga tccgactaga taagaaacct gtaaatagca tcaccacacc agtgactaac
     4501 atgtaactag taaataacag ggtcgtttca gctggcgtag cgtttaactg tgcagcaatc
     4561 gtttttaaga ttgggtctac tagacccact cccataaaag cgatcataca ggtagctgct
     4621 acaactagcg ctgaactttt cccctttgtt tcttcatttg tttgttcttg tatatctatt
     4681 tgattctcca atatttccat ccctttctct ctattagcat catacatgat ttattttaca
     4741 tgtaaatatg cacatgtaaa acgtggaaac tttactgtga tataataaag aaaataagac
     4801 gagaggtagg atttttatga atactttagc ctatatatta ttatgcgtgc ttttatccaa
     4861 accacgttct gggtatgaat tgaagcagct gatcacaatt ttttgggaag cccatcacag
     4921 tcagatctat acaacgcttg ccaagttaga ccagcagggt tatgtgaccg ttttggacaa
     4981 agacgagcac tctcaaaaaa agatctacca cttgactgat gaaggacgtg ctcttgtaga
     5041 ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa gatgaatttc tagcaaaaat
     5101 ctatgctttt tcgacgctcg ataaaacaac agctactgga ctgctattca ctagagaaca
     5161 acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg aatgacctct cgctagacaa
     5221 gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga attatgcttt gtcagcagga
     5281 actccaatgg tgtcagcaag taagagaaca aatgcagcag ttttttgagt aagggataaa
     5341 cagaaccttt ctagaaatat ctattgaaga gcaaattatc ttcaataaaa gagcaataca
     5401 gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc tatcgttcgg atcactattt
     5461 ttctagaact gcattcaaga gactatgaca aaaagcatgc caaggccttt tttcaaataa
     5521 cagtgttcca tcattcatta ctttgaattg cttttagcac acttcttgaa agcgggccaa
     5581 cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat tttccaagca ttcatataag
     5641 cagagaataa ttgatcaggt attccttgtt ccatgatcac accgaataaa gacatacatg
     5701 tcaccattcc taaaaccatc aagcatgaaa tagttaagat catttggacc ttgctttctg
     5761 gatttactgg tagtttaaag gcaatttttt tcgccagcac accgatgatg aagacatcca
     5821 aaatgaaagc caccacgaat ccaggcacta gaccaatcaa caaacttgat aaatgaagat
     5881 cttgatgcag ccataaatta tacgcgctca tccccaagac catcaaaaaa cacatcattg
     5941 ttgtaaaaat tattccttct tttttatttg taggcaatct gtaccactcc tttaaatttg
     6001 tcacgaaaat catcgtagca catttaaaaa tttctcgtaa gtactttttt tattttctgc
     6061 ccttttattt cataaacttc atcagccgta cgctcgacaa agtaagaatc atgagaagta
     6121 aacaaaatcg ttcccggata tatttgcatt agcttctcta aagcttcgat cgttgccata
     6181 tcaataaaat tagtcggttc atctaacagc aacacattac ttggcttagt aaaaagcaaa
     6241 gcaatcgtca gacgggtcgc ttctccccca cttaatgtac ataacggttt cgtcaaagtt
     6301 tcctgagcaa accctaagtg attcagcaaa ctacggacaa gcgattctga aaactctgtt
     6361 tgtctcatga caaaacgaat gatggattct tcagatgtca tttgataacc cttttgctga
     6421 tatacttgaa agcttacttt cggagagaga aggattcctt ctccttgttt gcggatatgt
     6481 tctaataagg ttgtcttacc tgaaccattt tcaccgacaa tcgcgatttt tttacctaaa
     6541 gaaaactgaa aatcaccatt taccagtaat gttctcccac ttctttccaa ttggacattt
     6601 tgtcccatga tgggatagcg gctgtggagt tcaagagatt ttggtactgg aaaacggatt
     6661 tgtttcatct gctgcggctt ggttgtttct cctatttttt ggagtcttct ctctaatgtt
     6721 ttcgcattct tttggatggc tttttgtaca ctgtcttttt gtttagagga agacaaccga
     6781 tccggtctga tttgttgctt tttcttttta ctggacactt tttccaactt ttccgcttgt
     6841 tttttctttt cttggatcga tttttccaat cgttttttct ctttcatgat cgactctgct
     6901 tctcgtagct gagtcttttt ctccaattcc ttttgagtga gataggcact ataattcccc
     6961 gaaaactctc ggatttctcc gtcttttact tcccagattt tcgatgctag ctcatctaga
     7021 aaatagcgat catgactaac aaaaaccagc gtaccataat aataacgaag ctcttcaatc
     7081 agtcgatcaa tactttttct atctagatgg gttgttggct cgtctagaag catcccttgt
     7141 tcataagaag aaagaagacg tatcaactcg atctttcgtt cttcccctcc gctccatttc
     7201 cgctctgaag tttcttccag tcttaactgg aaatgactgg ctaattccaa atttaggtcc
     7261 tttgcttcag cgagataggt taattgaggc aggtagttga aggtgatctc tgtttggatt
     7321 ttcccatgat cgggaaaaag ttcaccggct attagtttga gcaaagtcga tttaccgaca
     7381 ccattttctc ctatgatccc tatacgatct tgctgataga cacgaagaga atcaatagat
     7441 agttctagct ggttgccaaa gctgacttgt agatttgtta tatttactgc taaattttcc
     7501 atatgtttta cactcctatt tcatgtaaaa caacaattat cgatactaaa aaagagcaga
     7561 aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta ctctgaacga aataactatg
     7621 actaaatcac caaatctgtc atagctttca cctattctac tgtcgttcga aaaaatggac
     7681 atactccatc catgaaattg ttgtttatac atgtcttttg aatttattta gttgttattc
     7741 aaaagttcga aacgtaattt catcgatgca gtcatttttc cgacctcctt ttttattagg
     7801 ttaccacaca cgattcaaaa aaagcaatag ctactgaata gtaactgaaa accttttatt
     7861 cgaacgtagt gatcttttct ggtgctaaac gaacagactc gtaaccttct tctaccgctt
     7921 ttcctattga taaaataata actggaacat aacggtcttt atccaaacca aatgcctctg
     7981 ccaactgatc tgcttcgaat ccaccaattg gatttgtttc atagccatga tcacgcgcaa
     8041 ctaacatgaa ttgcatagcc gctaagctag aatcgacttt gactacatcg ttcatttctt
     8101 ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa ttgttgatct ctgacttctt
     8161 ttggcatctt ccctgattcg taagcttggt tatagatttc ttcgccatat tcgtaacatt
     8221 gcatatcgcc aaaaatcatg accattgcag aagaagtatc attttgtctc gtattgaaac
     8281 ggatcaaagg acgcaagatt tcttttgctt cctcgctttc agcgaccaca aagcgccatg
     8341 gttgcatatt gacagaagaa ggtgcagtcg tcgcttcttt gatcatttcc agcatttctt
     8401 cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga tcttctacca gtcataattg
     8461 ttgaaaaatc atttttttgt tccattatta ttaacctctt tcgtttctgt tgttatattg
     8521 caaagttggg tattcaattt atctaaaagt gttagaaatt gtttcatttc tacatcgctg
     8581 aaagccgggg atagcacgga acctgtatct ttatggtcca tttcacaatg ctcgattttc
     8641 ttctgggctt tttctgttag cgctacatac acttcccggt tgttctgagg atttctctga
     8701 cgcgaaacat agccatttgc ttccaatacc ttcagatgac gagtaactgc tccttgatca
     8761 atctgcaact tttcttgcaa actgctttgt agcaatggat tttcttcttt cagtctcatc
     8821 atgatctcgt atctagtaag acttataccg atattttctt caaagatctg gacgattttt
     8881 tgatcggcta atttgatttg atataaaagg tgactaatct cttctaaaga cataatttcc
     8941 tcctttctaa taattgactc gtcaacgatt gacttgtcaa taatagaata aaaaaagaaa
     9001 aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc aatagaaaag tatagagaaa
     9061 agaaattttt tgtagaatga acggaaaaaa tgataaagaa agcacaacgc taaaaccaag
     9121 attattgatt cttatcaaac tatagaagat agctcttctg cagtagaaaa ttttacaccg
     9181 ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg aatattagaa attggatcag
     9241 tatgcttaaa cagtaataaa aaagttatga caagatgttg tcacaacctt ttttgagtaa
     9301 acggtgttca aaaacttgca tctgcggtaa taagttcaac caaacaaaaa acatgaaaat
     9361 ctgtttttgt ttggctgttc ttactacttg atgcagggca gctttttcac tacctctttt
     9421 taataaccat aaaatcctat tccttcatat ctccagccca tcggtgttag ccaatctctt
     9481 tccttagcac tcaaggtata gtgatgggta ccagtttttt catttggatt aaatagtcga
     9541 taaattggct tggaaccacc agaataaaaa cttagacctt cataacgcca accgattttt
     9601 gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat ggtctccact gtaaggattg
     9661 tataaacggt agacaggttg tccgctgttt ggtgcttgcc aagcaatccc ttcatattcc
     9721 cagcctacag ttggcagata atttttttct ccggcattca atgtatataa atgttctcct
     9781 gtatgacggt tatacagacg atacatcgga gaagttgaag ttggtgctgt atcatactgt
     9841 gtcagattat aggtttcgat tacattgttc aatgtagaac cataattagg cgcagtggca
     9901 taacgacctg ttagccaagc cgtagcatct ttgtaagagt tcgtattgct tttccatgca
     9961 ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct tcaataccgt tacgttatct
    10021 tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg cattcaccgt aatccattgc
    10081 ccattcacat attccgaagt aggcatcgta acagattggc cattatagct tcctttaatc
    10141 ccaaatagat tgtaattagg ggcagaagcc aaagtactgt tcccccagcc actttcaacg
    10201 atggcttgag caatcatgac tgatgcatat aagtcattgg cacttgccaa actttgtgca
    10261 gttgaggcaa tactatcaat gaacgcttgt tgagaattag cagatgagcg agcataaaaa
    10321 gcatagccag tatagttttc gaattcagac aaatctgctt gatgggtatg tgtcaatgtt
    10381 tgcccatctg tcaattggtt cgtgatttct tctgcattct ctggattgtt ctgagcttct
    10441 gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt cagttgttgg ttcttcggtt
    10501 gaaattgtat tttctgagac agtagctgac tgcaaagtac tctctgtatc gttttcaatt
    10561 cctgtttctg aagaactcga tgtctgagtt gcgacagttt ctgtcgattc ttcagctagc
    10621 actgttgtgt tggcaactgc tagatttcct aaaagaataa cgctaaataa taaattcttt
    10681 gctttcatct tttttctcct ttgaattatg aaacttttcc taaataacag tgtaactaaa
    10741 aaagtaaaag caagtcaatg tccatattta aataacattt gtttcttttt gataaatttg
    10801 ctttttctac ataaaaagac gtaaatgaag aatattcttc tccattcacg ccttaaattg
    10861 atggtcgttt gaataactat ttaacaagta attctctttg gatattatcc gttatggatt
    10921 atacttcctg tgtcgtcgga cggataatca tgttactgac agacatccgg tctggcgttt
    10981 ctatcgcaaa tgctacagca gaagctatat cttcggaggt caatccccat tctttttgcg
    11041 cttcatgaag ttcaagagca gcctttttgt cgctgatggt ttgatataat tcagtttgaa
    11101 cagctcctgg agagatgatc gtagatttga tgttattttg ccgttgctct tgacgtaatc
    11161 cttccatgat tgcacggaca gcaaattttg ttccgcaata aacggcggaa tcaggataaa
    11221 caacatgtcc cgccacagaa tcagttgaaa tgatatgccc agatttttgt ttttccatga
    11281 ttggaagtac ggcggaaatt ccatttaaca cacccatgat gttgatatca agcatatttt
    11341 gccattcatc acggtgacct tcaactaatg gagcggttgg catgattcct gcattattgt
    11401 acaatacatc gattcgaccg aacttttcca tcgctaaatc gataactgct tggacttgtg
    11461 caaaatctct tacatctgct gtttgaatat atagctcagc ttctggtaaa gattctttta
    11521 ttgcttttag gcgatcttct cggcgtgcag ctataactag tttagcccct ttttctgcta
    11581 atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc catgatgaca atcacttttt
    11641 ctgataaatc attcattcta ttctcctcct gttttgaaaa atctcttgct tatactcact
    11701 atctcttatt tcatcttact gttcaaataa tccgttcgac aggtttgacg attttggcag
    11761 ggattcctgc aacgatggta ttgtctggga catctttcgt aacagttgca ttcgctgcaa
    11821 caatggcatt ctcgccaatc gttacgcctg gcagaatagt tgctcctgct ccgatccaag
    11881 catttttctt gatgtggatt ggctttactg acaagcctct tcgttctttt ggatcggtta
    11941 gatggttcac cgtgatgagg ttgacacgag gaccaatcaa tactgaatct tctaaacaga
    12001 tgccacctaa gtcaacgaac atcacttgac gattgataaa gacatttttc cctatactaa
    12061 tatgtttgcc gaaatctgta taaaaaggca gcagaacagt caccgtatca tctactttgt
    12121 cactaatgat attttctagc cgtttgcgga tatctgctgg ttcatggtac ccagtattca
    12181 gcgctgctaa aggctcttcg ttttcagctt ggatctgatg gatcacttca aagagcggat
    12241 ccgttactcg aatctcttta ttgacgattt gtttttcgat gaggcttgtc attttgctcc
    12301 ctccttttat ccttacttta taacggacgg cttatcatgt ctaatactta taaaacatac
    12361 ttaactattc cttttaacta tacttagtgg tatgcttttt taaaaacagg agggaaaaaa
    12421 tggatattcg tatactgacg tattttttag cagtagctag agaaaaaaat atcagccgag
    12481 cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa acaactgaaa gatttagaag
    12541 aagaacttgg tgtgacactt ttcactcggg gctcccgaga gatacagttg acggattctg
    12601 gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt ggtagaaaaa acggcgaaca
    12661 atttaacaaa tgacgcaatc atctctggag aaatctacat tggcggaggc gaaactaaag
    12721 caatctcttt catttctgat agccttcatt cgttgatcaa ggcgcatcct gatatccagt
    12781 ttcatttata tagcgggaat gcagatgata tcatagacaa actggatagc ggattacttg
    12841 attttggact cgtaatcgaa ccgacaaaca aacaaaaata ccagtcattt cgactgccag
    12901 cccaagatcg ttggggactt ttgatacatc gaaatcatcc tttagcaaag caaaccgtca
    12961 tccatccgca ggaactgaaa actgttcctt tgatcatttc ccggcaaacg tcagtggaca
    13021 gtcaactagc tgaatggcta ggtttcagca tagatcagtt atcgatcgtc ggaacatata
    13081 acttattgta caatgcttct ttgatggtcg ataacgggac ttttggtgca ttagctctag
    13141 atgggatcat caatacttat gggacggaaa aaatattcat cccactcgat ccgccattac
    13201 aagcttcact gaatctcatt tggaaaaaag accagcctct ttctcctgct gctcgtaaat
    13261 ttttagatgt cctaacggag aaatacagtt aagtagagtt tttttccttc gctttcagct
    13321 ttttaacaac gaataagata agcaaaagat ccaacgatta aataacataa gtttcttaat
    13381 ttatttcttc cttttatttc tgataattcc agtaaattac tataattata aaaaaagaat
    13441 tgacaaaaca taactgtcta tataaaattg attacgttaa caataattcg tttcatgtga
    13501 ctagatcata ctaggcatga aaaatctctg aggaacatgg tttccctagg aggtttttcg
    13561 tgtctatttt tgtttggccg caatcaactg gtctagcaaa cgtgaaaacc aaatcttcaa
    13621 aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa aatgcagtat tggtagatga
    13681 cggtggacag gaaaaagtag ctattggaaa agggattgga tttgacagaa aaagaaatga
    13741 tttgattttt gatcgtgatg tcgaacggat atttgtcatg gaaccagaag gtcaaatcaa
    13801 gttgcaaaat ttacttaatc agattgatga acgcttttta tttgcagcag aaacgatcat
    13861 tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa catgtattga tcgctttgac
    13921 cgaccacatt gctttttctg cggagaacat taataacggt atcgtcatcc gcaacaaact
    13981 tttaagagaa atcgaagtgc tttacagtga tgaattcagt attgctcaat gggcagtaga
    14041 gtatctgaca aaagagcttg ggattccata tacttatgac gaagcaggat atatcgcaat
    14101 ccatattcac agcggacgaa gcggtcggaa caataaccat cgcagtatcc gtgaagtgac
    14161 gattgtatct gacatcatcc atttagtgga atcagaattg gaaacagata tccattcaga
    14221 accattttca ctgaattatt caagactggt gaatcattta cgactgcttc tacaacgaac
    14281 ccatgcgcaa caatatgctg ttttagacac ggaaatcgta gaaatggtca aacgaaaata
    14341 cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg ctgaccaaag aataccagct
    14401 cgcgatcacg aaagaagaac tgggttactt ggccatccat atcgagcgtt taagaagcgc
    14461 aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa aattgaaagc atatctacaa
    14521 cgaatgggac gttcccttca acttccagtt gcggtcttac ctgctgctgc tttactagta
    14581 gggatcggaa actggtgggc atcttatagt aacgacattg tcgctcattt tttacaagct
    14641 ggcggtaacg cagttttagg tcaattacct ttgctgtttg ccgttgggtt agcactaggg
    14701 atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt tggtcgcatt tgaagttcca
    14761 gtaaatgttt taaaaactga ttcagtagct actttattga atatcaaacc agaatcagtt
    14821 gatccatctt ttgctcagat cagtaacgta tttattggta ttattgccgg attgattgcg
    14881 gcagctcttt ataatcgttt ccacgaagca aaattaccga tggctttatc tttctttagc
    14941 ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt tgctgatcag tgcagtgcta
    15001 ctgctcgtat ggcctccggt ttactctgct ttagtttctt ttgctaaatt cattttgagt
    15061 ttaggctggg gtggtgcagg tctttatggc ttcttcaacc gtttactgat ccctacagga
    15121 ttgcatcaag caatgaactc cgtgttctgg tttgacgtag caggaatcaa tgatattggt
    15181 aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa tgtatcaagc tggatttttc
    15241 ccaatcatga tgttcgggct tccagcagga gcatatgcta tttacagaaa tgcacgtcca
    15301 gaaaacaaag caaaaacagc ttccttgatg ctagcagcag cttttgcttc attctttact
    15361 ggtgtgacag aaccacttga attttcattt atgttcgttg cttggccatt atatgtcttg
    15421 catgcaattt tcactgggat ttctttagca gtgagtgcat tcttccattg gacagcagga
    15481 tttgccttta gtgccggatt tgtcgattac ttcctatctt tgaaaaatcc gattgcgaat
    15541 cagccattga tgttgatcgt ccaaggatta gtaacagcag tgatttacta ctttggcttt
    15601 aactttgcta tcaaaaaatt ccatctgatg actcctggcc gcgaggaagc agatctttca
    15661 gatgacgata ctgcaacgac taattcagac aataaatatg cggcacaagc aagcaaaatc
    15721 tacgctgcat taggcggagc cgacaatgta acttcgatcg ataactgtac tacacgcctt
    15781 cgtttgcaag tcaaagatac aggcctgatc gatcaaaata aaatcaaagc cactggcgta
    15841 cctggtatga agatcatcga cggaaaaaat gcgcaagtca tcgtaggtac agaagtacaa
    15901 ttcgttgcag atgagatggc aaaactacat ggtggtgctg cagcacgacc tgcacaaaca
    15961 aacaccgtgg ttaaaacaga aacatttgcc ggtgaaacga tcgaacaaga tatttacgct
    16021 atcgcaaatg ggaaactgat tccaatcact gaagtttcag atgatgtatt cgcggaaaaa
    16081 atgatgggtg acggctatgc tgtattacca gaaaatggag aaatcttctc tccaatcgct
    16141 ggtacgatca caaacatttt cccaacgaaa catgctgtcg gaatccaaac agatgctggt
    16201 atcgaagtct tgcttcatat gggcatcaac actgttgact taaaaggcga accattcact
    16261 ttatatgtag aagaaggtca aaaagtcgca agaggtcaat tgattgcttt agttgatctt
    16321 gccgctattc aatctgcagg caaaaacacg gatatggtcg ttgtattcac aaatggcgac
    16381 aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa aagcaaatga taaaatcggc
    16441 tctgtttcca acaaagcctg agccatcgtc ttccccagac ttttttatcg ataaacaaac
    16501 aggagctgag acaaaaatct tgtctcagct cctttttccg aataaaaacg gcgttctcct
    16561 gccaactccg cggtaataag tccagctaaa cgaaacatat gaagagctat tttttatttg
    16621 actcttctta ttacttgatg cagaacagct ttttcacaaa atttttcttc atactcaaaa
    16681 ctaatcggct ttttcacaac ctctttatac attattcctg atccaatatt tcttttaatg
    16741 ctgcttcaag agaagttaat ggttttccca atactttttc taaatcatca gaggaaatag
    16801 ctaactgatc ctttttaata tcatgttgga tcgctaaaaa catgtcagct gcttcttgag
    16861 ggaatccggc ttcttttaga ttttcgacaa acgctgcatc acttgcttca ataatttcta
    16921 actctttacc tgtcgcacgc tctaatgcct tggctaattc ttcatagtca gttagattgc
    16981 cggataattc taagatttct ggagagtcac tttctactag tacttttgct gcagcttctg
    17041 catattcacg tttcaatgcc caacctgttt ttccatttcc tgcagcataa acgaattgac
    17101 cagtttttaa agcctgccca atgataggta attcattctc tacataccaa ttattccgta
    17161 aaaatgtatg ggaaatacct gaatccttaa tcattttttc agtgaattgg tgatctggcg
    17221 ctaatatact gatcgagtga tcggcatctg caaaacttgt ataagcgata taagataccc
    17281 cagctgcttg tgctgccttc actacattct tatgttcggc ttgacgattt cctggaacgc
    17341 cagagacaaa aagcaatcga tctattcctt tcaacgcttc tttcattgag tctaaatcat
    17401 cataatcacc aatacgtata ttaaatccag cttctttcag tgcttctcct ttttcttcac
    17461 tacgtactag tacatagatt tcattttgtg gaaccatctt ttttaagaat tccagcgcat
    17521 aaccaccaaa acctccagtt gctcctgtta ctaaataatg catatttgcc actccctatt
    17581 ctttttattt gttaagattt acagctgtac atttataaat tacaccttta tcataaagag
    17641 tgtggtaaga taagtcaagc aaaaaaaatg aaaggatgac aactgatgaa gtattctctg
    17701 caatttagtg atgcaatcca tgtacttgcc tatattgaaa tgtttcaaga tacccgatca
    17761 ctttctagtg agatgattgc cagtagtatc gaaacaaatg cagcaaatgt acgtaaaatc
    17821 atgagtcagc tcaaaaaatc cggtttgatc tttacaaaaa ccggaaaacc gcagccttcc
    17881 cttgccaaaa gtccagatca gatttcttta ctagatgtct atcgcagcat cgaaggtaat
    17941 acacagctta tccaagtcga tccaaaaaca aatccaaatt gcgtcgttgg tgcgaatatc
    18001 caagaagctt tagaagaaag ctacgctcgg ctgcagaaaa aggcagaaga agaaatggct
    18061 aagatcacac ttgatagtct gatacataaa atagccgttt tggaaaaaga aaaaagacca
    18121 gaaaacacag gcctcgtaga gagattttta taattctctc tactgtgcct gcatttttct
    18181 gatcggaatc gtctacataa attcttttat tctccggggg acaaaagcct tgtcatgccc
    18241 ctttttcctc tctaatgctc aggtcaaagc gctgaagtca tgacgtcttg ataaacggtg
    18301 ttcaagtagc tgaccctcgg cattagttct gaaaaatgca aaatatggaa agctatttcc
    18361 catttttcct ctctaatgct caggtcaaag cgctactttc acgacctcta tattatcatg
    18421 gtttattgac taccggtatc cagatttctc ccatatattt cccgttggta actcccattt
    18481 cgactacagc atttggaccc ccaacatagg cgtaatcttc tacttctctt aaaacatgcc
    18541 caaaggccgt attcgtcaaa tcatttctta attcttcagg cgattctgct aaagcgctta
    18601 caacaagata cgttcctttt gggaatggga tgattcgcgt agtgtttgga acatcctgac
    18661 tcgtttgaac accgacatag tacatcattt tcccgttata gggttcgttg actgtaaaga
    18721 aataatgatt ttcagcagct tgtttcaatg catctattgt tccattaccg ataacttttt
    18781 gctcaaatgc ttgtttctct gcagccaaac cgacaaaatc cgtattgcta ctcttcaatt
    18841 ccacaccaat acccgtcacc gtaaaatctg gtttttcttc gatcctatac tttgccattt
    18901 ctcccaccct ttctattata ttaagttcat cttacataaa attaaaggga aatgacacga
    18961 ttacgctctt ttttctaggg atatttttat tttttaacta tttttttgct ttcactgcaa
    19021 tccaaatctc actgtaaaca ttactgaaat cctctcccca ttttgtaaaa gagatctcgg
    19081 gagcttcgac taacccataa tctgaagaag gaagccattc tgcatagatt ttcccccaag
    19141 tatcttgtaa agtttgggga aatggccctt tattaggaaa aatggcccaa gtgtgttctt
    19201 ctatagcaag catttctaaa tcagcgtacg gattttcttt tgtcgtagca aagccaatca
    19261 tgtgcatcaa actgcccttt tcttccaagc ggtctccatc aaatccataa gaaacgttga
    19321 ttacttgatt tggatataaa tctcctaact tatgcatctc ttccctttga gtctccgtga
    19381 tcgtctgcgc taacttttgt atttcttgat tgactccttc aaatttgaca ggaacttttt
    19441 tcgccacacc tgccacataa aactgttctt tcttttcgat acgaaattcc atcgattttc
    19501 ctcctctgac atcaataaaa aatgtcaagc gtggaaaaga tttttgtatg ccgtatctac
    19561 tgatttctga aggcagatat cctgaccaat cacgaaaagc tcttgaaaaa ccttcgactg
    19621 tttcatatcc atatctaaac gccgtttctg tcactcttga tccatctaga agatcgcgat
    19681 tagccaaagc caatctacgg tttttgatat agtccattag cgacatccct gcaataaacg
    19741 taaagatgcg ttttaattga tattcagaga ttcctgtatt ttcggctatt attttatagg
    19801 aaagtccttc tgtcaaatgt tcttcgatgt aatcgatcaa ttggttcaga tctttaagca
    19861 tatttatctt cctccttgat tgccttttta tcatattatc aaagaaagtc aattacttga
    19921 tgaatcagga accaatttag tcagatgtag aaaatgttgt atggatctaa agaaaattct
    19981 caagcagcgt ttcgtaacgg ataattggta ttatctgcct atctatgttt tacctttaga
    20041 aaaataaatt ttggggaaga cagattctaa gtaaacaaag cggtaaaaat atcttttatt
    20101 cccttagtta atccgcttac tacctactgt acaaagcaaa ttgttgttca aactgctgat
    20161 cagaacgctg cttggatttt tctttgcgat acatcatacc tcttttatca attggaacaa
    20221 ttatttattt ctaacaaaat cacttcattt ttgtcagata aataaaaaac aaaaaaagtt
    20281 ccatctttaa aacacgctat tcttttactt tttctcacat tctctaagaa aagaaaagga
    20341 gaatgaattt ttgacaattc gagcaaaagt gattatcaaa tatgtaaaat cagctacttt
    20401 tttcttaaac gtttctttat actaaatata aataagtgtt tcatgaaagg agaactagat
    20461 gaataattgg cttgagtatt ttcctgaaaa cgttttggaa cgcggttaca gctatcatct
    20521 gcacggtttt gtccgtcatt tgaactatac aagcaagtac ttgtccgcaa cagtttctgg
    20581 cacggaagat tataaggtag tgatcacttg ggatgaaaag accaacatga cttgtgattg
    20641 tctatatgcc atagaaggaa aaaaatgcaa acacatggct gctgtcttat tcgcttacga
    20701 agaacgtcca ataaaaaaat ccaactactc actttccgaa ctctcgtccc ttgtttcctc
    20761 cgctagttcc tctcttgtac gggagctttt aacagagatt cttatagaac atccgcactt
    20821 tatcgaacgc ttcaaagtta agatgccatt tcatgcaata aattattcag ataaattaac
    20881 aactatcatc cataaatacg atcatattat caagaaaaac aaaaacagga aaacagcgaa
    20941 gttcatcatg gagatgcgaa agttcatcca agaagcagtt gaatcgctta ttcagcaaaa
    21001 tgcctatctt cccgcattcg aattgatcaa tgaagtgatt gctactctcg aaacattcta
    21061 ttgggagcct gaggatgaac gaacccttct tctgatagag gactgttatt atttatggaa
    21121 agaattatta gcagaagcac ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg
    21181 ccaagtcgat catactgacg cctcttattc aaaacgctac tctataaaaa tcctgaaaga
    21241 agactttcgg gaaaaagaat tttctaacca aaagaaaaaa atagataaaa aactaaagaa
    21301 acggtaaaga aagacgattt caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa
    21361 tccacaagaa atacagcaga ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa
    21421 agaagactta acgcgtaccc aatattagtc atttgactat taggtacgcg ttttgtttta
    21481 taagtcatga caaatttcct tcgttgctgg atagattttc cggcattgtt gatagatttt
    21541 atgatacttt tctacatttt gtaagagagg ttccgctgca tctgtatatt ggataaaatt
    21601 ctgacaacaa ctttcaggtg tgtcgaacca ccctaaccct agtgcagcaa tcattgttgc
    21661 gcctaatgca ggtccttcct ccacacttaa acaatagacc gttgcatcga aaatatcagc
    21721 ttggatttgt agccatgcaa gatttttcgc gcctcctccc accgaaacaa tacgtgtaaa
    21781 tctgcgaccc gctagttctt tacgtttcaa tcccaccatt gacaaatgct cattaaaaat
    21841 ttagaatatt cacaagttta acgacaatta tgaaaattta tgtcgtgcta aaggagtttt
    21901 gttattggat gaaaagttag atataaaaac tgcgatcaaa gacacgttac ctaccgtttt
    21961 cggttatatc ggtattggac ttgcatttgg tatcgttggg aaagctgccg gatttcatcc
    22021 actagtcgtc acgttgatgt ccctgctggt ctatgctggt tctgcccaat ttatcacagt
    22081 cagcatgctt gctagtcaca gcccattgct ttccatcgtt ttctcgacct ttctagtcaa
    22141 ttcccgaatg attctgatga gtatgacgat tgctccttat ttcaagaaaa atcggctgct
    22201 tcaaaatctg ctgattggca cattgctgac agatgaaagt ttcgcactgg gaatgaataa
    22261 attgaactat actggacaaa agttaaactt tcgttggatg aatacagcta attggatttc
    22321 ttatttgacg tgggtgggtt cttctcttgt tggcgcactt ttagggaatt ttattactga
    22381 tccgaaaaag tttggtttgg atttcgccat cgtcgctatg tttatcgggt tactttatct
    22441 tcaagtcatt tctgatagaa acacctctaa acgattacag ttgatcctga ttggtctcac
    22501 tttgattctg gtatatgtcg gactgatttt tatcccaagc aacttagtga ttgtagttgt
    22561 aacactgatt gggtgcggat tgggggtgtg gatcaaacat gccttctttt gagtttacat
    22621 tgctgactat tttaggttgt actttagcaa cttggctttc tagggtccta ccatttgtct
    22681 tactgaaaaa attcgattta ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg
    22741 tcattatgtc tgctttatgg ttcagcagtc tatttaccca aaatatcgga catctgccgc
    22801 agatcaatct cgaaaatgct ttggcttcag ttcctacact tttagctgct attctttcaa
    22861 aaagcttatt ggtgatcgtc ttggcaggaa ttctttccct ttcgttgatt cgcttattat
    22921 aaataaaaaa acaagagctt atggcaaatt actgtcatgc gctcttgttt ttatcaaagt
    22981 caaataagaa aaaaatcggc gtattcttgc ttatccgttg aacggaccac taacaacgac
    23041 ttttcctaac atatgtcctg tttctacgtc tttggttgct tgtttcaagt tcttcgcatt
    23101 catcccatct gaatagacct tgctcaatgt actgcggata atcccttcat ttgctaaagc
    23161 agcaatgtgc gctaaagctt ctccttgcgt ttcgatttca tagttatagt ctgttttagc
    23221 gaacatatac tcccagtcga agctgataga ttggtttttc caaacaccta tatctaacgg
    23281 ctctttgatc cctacgatcg ttcccacatg tccaaatggt cggacaaggt gcttgatttg
    23341 atcaaagtag cgggtaatat cgaataatac agcgacatac tctacttgat cgattcctaa
    23401 ttcacgaaaa cttttactta agtcgtgatg ataatcgatt ggatagtcta cacctgtttt
    23461 tttcagccat tcaaaattct tcggacttgc agtcgcgtag acttctaggc cagcccaatg
    23521 cgctagttga ttcaaaatcg aaccgacgcc cccggatccg ttgatcacta agattttttt
    23581 cccgcgattg gcattttctt ctggagttag accaaatttt tcaaataaca attcataagc
    23641 ggtcaatgag gtcaacggca aggctgcagc ttcttcatct gaaagatttt ttggtgcaag
    23701 agcgacaata cgttcgtcaa ctagttggta ttcttgattg cttcctgctc ttgttgttgt
    23761 acctgcgtaa aaaacgcgat cgccgacaga aaatttttgc acttcgtcac ctacagcaac
    23821 aactttcccg actccatcaa aacctaagat acgtaaagca tttcttatcc cgttatcttg
    23881 gcgaagtttc gtatcgacag gattaacaga tactgcgttg acttttacca gaagatcccg
    23941 tgaactgggt acaggtatct gttctttttc atcaataaag ctttttggat catcgatcgg
    24001 caacccttca taaaaaccga ctaccttcat taattccttt tctgacatac gaattcctcc
    24061 ttagcttcat caagttggag catccataca gtgttcattt caatatatat agtatgctat
    24121 ttgttccacc ttttttattc atcataatta ttctcttact taaagtaaac tatttgcact
    24181 tttttggtta aaaaagaggt tgcgacaaga cttttgtcac aacctcttat aaacggtgtt
    24241 ctcctaccaa ctcttcggtc acaagtcaaa atattcatca aacatgagaa gctgtttttt
    24301 gaaattgctc cttgtgactc agagttaaac ggcaggctca caacctctga tataaacggt
    24361 gttcaatcaa ctgaccctcg gtattagctc aaaaaacgac aaaatatgaa gagctatttt
    24421 tgtttggctg ttcttattac ttgatacagg acagcttttt cacgacctct aattcacggt
    24481 gttcaagcaa gaaatcattg ttcactgaaa agcgttggat gttctttttc aaaggtctct
    24541 aagatctctc gtacttcatc agtagatttc gtattcatca actgaactcg caagtcattt
    24601 gctcctggaa atcctttgat atagatttta aagaaacgat gtagtcctgt gatggaacgc
    24661 gggatgattt ctgaataatg atcttgcaga tctagttgca gacgaagtag tccgatcagt
    24721 tctttttctg tatgttccct cgggtctttt tcaaatgcat aaggattctt gaaaatccct
    24781 cggccaatca tgatgccatc aacgccatat ttctcggcca attcttctcc tgtctggcga
    24841 tcggggatat ctccattgat cgtgattagt gtttgtggag cgatttggtc acgtaaagca
    24901 atgattttag gaatgacttc ccaatgtgca tctactttac tcatctcttt acgcgtacgc
    24961 aaatggacag aaagattggc tatatcttga tgaagtaggt gactgatcca ttcttccatc
    25021 tcagacattt ccgtatagcc gatacgcgtc ttgacgctga caggcaaacc gccagcttta
    25081 gctgcttcaa tcaattgtgc cgctacttct ggacgaagga tcaagccact gccttttcca
    25141 cgttctgcta cattagggac tgggcagccc atgttgatat cgattccttg gaagcccatt
    25201 tctgccatag caatgctcat ttctctaaaa aattctggct tgtctcccca aatatgggca
    25261 accatcggct gttcatcttc tgtaaatgtt aatcttcctc gtacactgtc ttttccatct
    25321 ggatgacaga aactgtctga gtttgtaaac tcagtaaaga agacatccgg tgctgctgca
    25381 tgctttacga catggcgaaa tacaacatct gttacatctt ccattggtgc taaaacaaaa
    25441 aaaggctttg gcagctcttt ccaaaaatta gtagtcattt atttgttact ctccattcta
    25501 tcaagcagtg aagtcgcaat ctttctcaaa aacattgtca ttatactagg aaacaaaaaa
    25561 agaagcaaag tgaacagctt aaaagatgtt tctgattgag caaaaaaaag actgtgacaa
    25621 aaatcttatc acagtccatt ttccggaata aatggtgttc aaaagcttgc atttgcggta
    25681 ataagtccaa ccaaacgaaa aataggagga gctatttttg tttggctgtt cttattactt
    25741 gatgcagaac agctttttca caacctctcc actaaacggt gttcaatcag ctgaccctcg
    25801 gtattcgctc aaaaaacaac aaaatatgag gaactatttt cccgtttgtt tctctaatac
    25861 tcaggtcaaa acgctgattt cacaacctct tttctaactt ctcaatcctc ttcctttatt
    25921 gatcaaatac caagtaacgg cgtacaaaac aacgatgaat aacacaagta cgatcatcga
    25981 tgttgctact ggcacatcga tcgttccaag gaatccgtaa cggaaaccgg agatcatata
    26041 aacgatcgga ttgacttttg aaacagcttg ccaaaagggc ggtaacatag agatggcata
    26101 gaaaacgcca cctagataag ttaacggctg caagacaaat gtaggcacga tcgatacatc
    26161 atcataagac tgagcaaaaa tcccgttgag cagtcctgct aatgaaaaca agattgccgt
    26221 catcaataat gtaatgatga ccatgaacca tgaatacaca tgaagtggta caaagaacaa
    26281 ggaaatgatc gttacgagag agccgacgag aatactgcga cccacaccac caatcacaaa
    26341 gccccaaata atcacatgcg taggaacagg tgctaccaag atttcttcga tatttttctg
    26401 aaatttttga gagaagaagg acgaagaaac attggcatac gaactagtga tagcagacat
    26461 cataatcaaa ccaggaacga tgaattccat atatgaaaaa ccacccatgt caccgatccg
    26521 cccaccgatc atttttccga aaataacaaa atacaaggat gtcgtgatca ctggcggaac
    26581 cagtgtttgg acccatatac gcaaatagcg gttcgtttcc ttgatagcta aactttttaa
    26641 tgcagtaaaa taaagattaa acatttttct cctccgtatg attattttct tctgtgattt
    26701 tcaagaacag ttcttctaaa cgattcgact tattcctcat agaaagaact ttgattcctt
    26761 gtcttgtaag ctgatcgaag attccgttca ccccttgatt tcgttcaact tcgacagcga
    26821 ttgtctgctc atcttcaaat gtacaagcat atccttcaat cacaggtttg gtatcatatg
    26881 gcgctaaatc aaagataaat gtttcaaatt gcagttttga cagaagctct ttcatacttg
    26941 tattctcgat cagttcaccc gattggatga ttccgatgtt gcgacaaagc atttcagctt
    27001 cttctagata atgcgtcgtc aaaatgatcg ttgtaccatt ttcgttcaat tctcgtagaa
    27061 actcccacat ctcgcgtctc aactcgatgt caactcctgc tgttggttca tccaagatca
    27121 gcaaacgtgg ttcatgcatc aatgccctag caatcatcag tcttcgcttc attcccccgg
    27181 aaagcatacg tgcacggaca tttctctttt cccaaagatt ggactgcttc aaatattttt
    27241 cgctgcgttt catcgcttct ttgcgagaga caccgtagta gcctgcttga ttcacgacga
    27301 tttgctggac cgtttcaaaa gggttaaaat tgaactcttg tggaaccaat ccaatctgtt
    27361 gctttgctcg aactagatcc ttgtccagat cataaccgaa gacattcact tttccagatg
    27421 ttttattgac taaagaagta ataatcccga tcgttgtaga cttcccagct ccatttggcc
    27481 ctaatagcgc atagaaatct ccttctttca ccgaaagatc gattccccgc aatgcttcga
    27541 ccccacttcc atagatcttt ttcaaattat ttatttcaag cgcatttgcc atttatttcc
    27601 tatctcctta tagacatgat tcttaattgt atcatggaat acagcagatg aaaaaacaac
    27661 ttggcttcca cacaaaaaca cacctgctaa caaagaggag atgtgttttc tagttcatga
    27721 tcatcttcga tagaataact atctaaacaa ctctggaact tttaaaatca taaaagtacc
    27781 gacagcaact actacgatga catatagaag atccttataa ctgaggttct tgtctttttt
    27841 attcatgtta agctttcctc cgtcctttta tcgaaagctt ttttctttta ttttaaccga
    27901 aataaataaa atggaaatag ttattttcaa tttctgtttt cgctgtttgt tttacggaga
    27961 actttgccat ggaacgtgcg tctcgtcgct gatgctaagt tttttcgata aaagctgcta
    28021 gaaggaagac agcgtggttt attttgtatc gtcttttgct aaatcttcca agatcatctg
    28081 gacagcagac tcgttcccat tgaattctgc cacttttttg gcatcaatgt acttcttttg
    28141 ataatcgtct ttttggaccc acgcatcatg cttcgtttga taaagttaat caaacggata
    28201 ccactgacgc tgttcatcga acaacaaact ttctttttgg gcctgttcaa ctatggattt
    28261 aggataattc aaaactttca gcaaggcaat cgcatttcgg gtccttgaag gtccttcttt
    28321 taatttgaaa tcaaaactga ttccttttcc ttctgttact tgttcttcaa agtggacatt
    28381 ctcacaataa ttctttaaaa tctctgtcaa ttcgatatca tgtgtggcga caaatgctaa
    28441 agatggatac tccgcaagcc accgaacgac tgaagcggaa gaagcaatcc gttcgatcgt
    28501 atttgttcct tttaaaatct catctacgaa gcagtagcag cgttctttgg aagccacttg
    28561 gtccagcaaa cgtttgatcg atttgatttc tgaaacaaaa taactgtcgc cttcaaataa
    28621 atcatcttcg attgccatgg aagtcaacac atgtccaggt tgcagggtaa actgttctgc
    28681 aatcgctgtt tggatcgtct gtgctagaat acagctgatg gctatacttt tgacatacgt
    28741 agattttcct gatgcattgg aacccgtgac taatgtattc ttctgccagt tgactggatt
    28801 aactaccgct tcttttaaca atggatgata gatatctttc gctgtcacac cgccctcagt
    28861 aaactctggt cggcaagtga tcggcatata ggtgcgataa ttcaaaacgg ctgctgcaac
    28921 ttccaattct cccagcaatt cccacagctt gatggcttca gttgaatgct tttctaattt
    28981 agtgagaacg aaatgatagg agataaatgg cagcatgaac atgatgttga catattcaaa
    29041 aatcaattct gcttcactcc cgctcttcat tcgaaatgaa aaacctaatt tagggatgtc
    29101 tcttaatggt ttagtttgct gtttcagctc tttttgaagt ggtgtcgtca tttttgctaa
    29161 ctgattggcg gctgaaatcg ttgaaaccaa ataactcatg ctattcagct ctgtttctag
    29221 tgtttgtttc ttcactagat aatagaccgt attgaatact aagccaccga ttgctagata
    29281 gattcctagt acttgcccta aaagaagcaa acctacaccc accagcggaa gcaagccaag
    29341 aaaaataaat aaacctacgc gtccaatctt tttacttttt ccatccgcta gatattgttt
    29401 tgcaaaatta tgatcttttt ttcctaaacg agcgaattga tattggattc tttctctcag
    29461 ttgaggattt tcctgataaa aagcaattag tttttctaac tgataatctt ctccaaaatc
    29521 aaagctacgt aaacgctgat acaacgcttc tgaacctaca ctagaatagg tactgttgat
    29581 cccttcaaat acttcgaaca tatccaagtc ataccaagta agatcgtcga tttcactatc
    29641 ccatgaacgg aattttttct ccgtgagcca cgcatccttc aaactttctt ctttatcaaa
    29701 tctcggttga taaggaattt ttccccaatt actacgaact gtttccttta ctttcaaacg
    29761 tgaaaaaatg tctaaaacaa ctaataaaat aacaactgca ataataatta aaacgataaa
    29821 ttctctttgg cccatatccc tctccgttcc ttgatttgtt agttttagta tagcactgtt
    29881 gcgctatttg aaaagggggt acaaaaataa ctgtcttcgt tacctaattc gtatcaatca
    29941 caccaaaata aaaacacctt ccaaaaaaga agatgtcttt aaaacgagat acaatttttt
    30001 gtcattcact tcaggtaaat tagagccaat taaaccctcc gcaacttgac ggagatggag
    30061 aagatgcccc cgtctaatct agcggtcatc tctcctccca ttccttccac caattttttt
    30121 gacaggtaaa gcccgattcc gctagattcc gtatttgtgc ggctggtatt ttcggtataa
    30181 aaacgctgga agactttttc aacagaggtt tgcgctcctg gtttgatagg attggccact
    30241 gttaatcgaa tgaatgaatc ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa
    30301 cgcaataaat tctgtatgat attttgcagc actcgcagca acatgagcgg atctgctaat
    30361 acagttgtat ttatcgagtg ttcagcgaac gctacctcga tatttttctc agaaaattgt
    30421 tcataaaaag agaaaaatac ttcttccacc tctttagaaa aagaaagcgt atggagatcc
    30481 atttcttttt gcttcgtctc tattcgagca agatcataaa actgatccgt catactgagt
    30541 aatcttgcaa cagattgttt gatgatcgtt aaatacttct cccgtttctc cgtatcctct
    30601 acctgttcca gtaattgtac ataaccgtta atagcagtca aaggagttct gagatcatga
    30661 gaaaggccga cgatcgatag tctgatgttt tcttccattt cttgaaatgc ttgtcctttt
    30721 tgttcatttt catccaccca tgtattcagt gcatctacta ggtccattag attcttttct
    30781 cgaaattcga ctgaaagacg accaccatat tcttgtcgtt cagaaagagt ggcgattttc
    30841 ttagttaatc tcttgatttc cctttgcagg aagaaaataa aacttcccat aaagataaca
    30901 acgagaagca acagccactt aatcaaatct aaaccccact ccccacaccg tatgaatata
    30961 cacagcatgt gcctttgcta gtttccctcg aagattggaa atatgcatat tcactgtttt
    31021 ctcactatca aaatatggtt cttgccaaac tgtttcataa agatttgctc gcgaaaatac
    31081 ttttttcgga tgttctaaaa aaagcttcag cagatcgaat tctcttcctg tcaaatggac
    31141 agccacacca ttcacattta catccctcga ctctttgtcc agtaagattt ccttgtaata
    31201 gatgcgttgc gacagatctt ttacttggac agaagtctga tgacgaagtt ggatgccgat
    31261 tcgtaacaac agttcttcga tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc
    31321 caacaaggaa actttatctc tttgatcgct ttttgctgaa agaacgatga tcggaacatt
    31381 cgatgtctgt cggatttctg ctaacaatgc ttcacctgaa agccctggaa gcatgagatc
    31441 tagaaggatc aaatcgacag gatatgtgct taacaataat tttccttccg tacctgaata
    31501 agcatcaaga agtttgtatc tctgttcaag aacctcctcc agtaaagaat gaacagcttc
    31561 atcgtcttcg ataatcaaaa tagtttccag caggaacacc tacttaatgt cagatttttg
    31621 gaaaataaac attcctagag ctgatgatac cacgattgtt actagactga ccagaatcat
    31681 tttcggtgct tgcgaataag ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc
    31741 cgtaaaccac tccgttagaa attccaagcc tttgattttt gcgaaaacac cgggaacgaa
    31801 ggccgttccc atgatataaa gaaaagaaag aagcaaactg acactggtac ttctagtgat
    31861 gaatgtcaaa gccactggaa cagataatgt cgctaatatc agcagaagca atacaggaaa
    31921 tacttttaga acataatttc catagttttt gattccctca acttcaccaa atccgaaagc
    31981 aagtgtgtaa gcaatcatgg ctagccccgt aatcaaaacc acgccaagtg ttgtgatcaa
    32041 aagcgtaacg agaagtttcg acacataata agtcattcga ttccgtccta aagataacgt
    32101 attgcgaatc gtaccgtttt gaaattcttc cccaatataa aagcacgcaa atgctgcgaa
    32161 agccagaaga atccatgcag ccattttttc caccattaaa atccctgttg caccgttgat
    32221 gactgtatcc gttgcttggt ttaattcatt gccgtatttt gtatgcatat accattcgtt
    32281 ccaagaataa aaaagcggaa gtacgaccat cagtcctagt gagacccaca ttttacggct
    32341 cttccatact tttatttttt cagcagttaa tagattaaac atgtggattg cctccagtta
    32401 attgtaagaa ataatcttcc agtttttgcc caactaggtg gataccatcc acaggaactt
    32461 ttgcctctac caatgcttga ttgatggctg ctacttggtc aattccttca aaaagctgga
    32521 taacacggct agattggaca aaataatccc tataaccaag agagtcgaga accgttactg
    32581 cttgttccgt cgccgaagtg tcaattttta taaattgacg agattcttgt gccaattctt
    32641 ctttcgataa ttctttgatc aagtgtcctt catgcaaaat accgtagtga gtggcgatct
    32701 gtgacaattc atctagcaag tggctagaaa gcaagacggt gatgccccgt tcttttacca
    32761 aacgttggat gatttctcgc atttctgcca tccctgctgg atccatccca ttcgtcggtt
    32821 catccaaaat caaaaattct ggattcgtga tcagtgcatt tgcaatagct aaacgctggc
    32881 gcatacctaa agagaaattc tttgctttct ttttccctgt attttccaat cgcatcattt
    32941 tcagcaggtc ttcgatttct ctatccgaca caccgccgtt cgctgcttgg atacgtaggt
    33001 tatctcttgc agttaattct ggataaagag caggggtttc aatcgattga cccatacgtc
    33061 gtcttgctgc ctgcaaatcc gtcgttccaa ataattcgat cgaaccttca tcaatcgaga
    33121 tcaaacccat aatagttctc ataaaggtag acttacccgc tccattctcc ccaatcaaac
    33181 catagatcat tccacgtttg atttcgatcg atactttatc taatgctttg gcagcaccgt
    33241 attttttggt tataccagtt gctttcaaga ccgtttcatt tccaaaatcc atacttatcc
    33301 ttctttctta ttgataagtg aagtatagtt gacgggtata aagaaagcag ccagaaatgt
    33361 ataaagaaag tgtaaatttt ccaattgaat ttcaagaaaa atcagactgt ctcgcagacg
    33421 tggttgagtt gtctgccaat caaagagcgc accaaacagt gctaatccaa gtggactggc
    33481 caccatggta atcgttgtta tcgttgacat cagacgtcct tgtatatgcg ttggaacttc
    33541 tcgctgaagc cagaccatat aaaaagcaaa agaaaacatt aatccttaaa aacggactta
    33601 tgaaaaataa tacatgccgt ttgggattta caattagcta ccaaataaga ggttatgaca
    33661 aaagactttt cacaatccct tcttccgaat aaacggtgtt ctcctgccaa ctcctcggtc
    33721 acaagtcaca atatttatca aacatgagaa gcaatttttg taaattcctt cttatttctt
    33781 gaagctgacc acttctgtca caacctcaga agcggataaa taatattgtt tgatgatact
    33841 ttatcttttt cccgtttgtt tatagatcga atgatattat agaccaagaa tcctacctat
    33901 taacatagct aaaaacacaa aaaaggcact ttccaaattg tggaaagtgc cagaaaagtt
    33961 aggattaagg taactgcgct cagcattgct tcgcccagta cgattctcgt aactcgtgaa
    34021 ctcgaacgat aatcgcaatt aacgttttga gaactgtgaa gctttacggg ctttcttaag
    34081 acctggtttt ttcttcgtat cattgaataa gtttgaaaaa ccttgattta taaaggtttt
    34141 cccgtaaata tattttatta tatttgatta aatttgaaat gtttgtgttc taaaatgtgt
    34201 tctaatttaa aggcagtata tttcgtattt tagattaaat ttttaggctc attcattctc
    34261 aaatccattc tattcagtca ataaaaattt taaaaagact ttataaacaa agatgttcaa
    34321 gcgagcatct tatttttttg tgcttaattt gaaaggagaa catttcatgt ataaaaaaca
    34381 aaaaaagaaa ttatgtgtat taactatttt gagtttggct gttcaagcaa ttgttggagc
    34441 aattgctcct acgattactt ttgctgatga aattacccat ccacaaacgg taaccgtaga
    34501 actagattta gcacatcaat atgctgttga aggaactttt agtgatggtc gtccaatgtc
    34561 agaagtcact gttccgcatt atgcagtgta caacggcttt aaacaagata ttttctgtat
    34621 tgaaccagga gtcccgattt acaatgagtt cactcctggc tatgagaaaa acccgttacc
    34681 agatatgtcc gataaagcca aattagtttc cgttttatgg aaaaaagtag gtacagatgc
    34741 agatactcat attgtggcac agaagatgat ctggcaagaa gttaatgggt atacccttca
    34801 ctcaataaaa cgatcagatg gtagtgccgt aaatatcgca gccattgagg caaaaataaa
    34861 tcaagccatt gacgattatc agaagaaacc aaccttccat aatagtacgg ctaaaacagt
    34921 attagggcaa tcgactactg tgacggatac gaataatctg aatttgtcag agttcgatga
    34981 ggttgtggaa aatgccgcaa acattgatta tcgtgtgaat ggtaatcagt tgataatcac
    35041 cccaaatgcc aattcaaaag aaagtggtgt gctgacgctt aagaaatctg ctggtaccgg
    35101 aacacctgtc gcctataaaa aggctggcca acagacttta atggccgggg cgattgataa
    35161 acctaatacc tacacaatca aaattgatgt ggaaacggag ggttctttaa agatcaaaaa
    35221 agtcgataaa gaatcgggtg atgttgtacc aggaacggtt ttccatttag actttggaaa
    35281 aactttacct gcaaaagacg tgactactga caaagaaggc attgctacat tggatgagat
    35341 tcctcatggt acaaaagtaa ccattaccga aaaatcagta ccggcgcctt atacgattga
    35401 taccaccccc atgactacca ccattaaagc aggcgaaacc atttacgtga cttcaaaaaa
    35461 cgcacgggaa aaagggcaaa tcattctgga taaaagcgga gttgaaacag ggagtgatct
    35521 ttggaatgac aattattcct tagctggtaa tacgtttgcc attcgcaaag acagtccaac
    35581 cggtgaaatc gttcaagaaa tgaccacgga tgaaaacggt catgcggaaa caccaaaaga
    35641 gattgctaat gcgttggaat tgggaaccta ttatgtgacc gaaactaaag ctagtcatgg
    35701 gttcgtgaat acgttcaaac ctgtaaaagt cgaattgaag tatgccaatc aaaccgtagc
    35761 ccttgttacc agtaacgtaa aagggcaaaa tcaagaagtc actggcgaaa ccactttgac
    35821 gaaagaagac aaagatactg gggataaagc acaaggtaag gcagtctttg agggtgctga
    35881 atacaccttg tttactgcta aggatggaaa agctgttaaa tggagtgagg cttttaaacc
    35941 agagttagcg aagggaacga aaggttctga tgaaacggtc actttgactt tagacgaaaa
    36001 gaaccaagtt gccgttaaac atttagctat caacgagtat tattggcaag aaacaaaagc
    36061 gcccgaagga tatagccttg atgaaacaaa gtatcctgta tccatcaaaa aggtggatga
    36121 tagcgaaaaa aatgccgtga ttacccgaaa tgttacggca aaagaacaag tgattcgctt
    36181 tggctttgat ttctttaaat ttgctggatc agctgcgggc actaccgaaa ctggcttcaa
    36241 tgatctgacc tttaaagtgt cgccattgga agggaccaat gaaatcacag gtgccgaaga
    36301 tgaagcgacc acagcttata acgagcaatt agggtttgat ggttatggca agtttgaaaa
    36361 tcttccttat ggggattatt tacttgaaga agtagaagcc ccagaaggtt ttcaaaagat
    36421 tacgccatta gaaatccgtt ctacttttaa ggaaaacaaa gaagactttg tgaagagtga
    36481 gtatgtcttt accattaccg aacaagacca aaaacaacca attaaaacgg tgaccgttcc
    36541 ttacgagaag ctgacgaaca aagcattttc tgttagtttg aaccgtttaa tgctctatga
    36601 tttgcctgag gaagaagata gtttaacttc tcttgcgact tggaaagacg gaaacaaaga
    36661 attaacttct ctggattcta ccgagctagt tgataaattg agttacaatc tacatgaaat
    36721 caaagaagac tggtatgtgg tagcgcaagc cattgatgtg gatgcgacaa aagctgccca
    36781 agaaaaagat gaaaaagcca aaccagtggt gattgcagaa acaagcgcaa cgttggcgaa
    36841 caaagagaaa acaggaactt ggaaaattca gcataaatta accgccgaac aagttttaaa
    36901 taaaactatt gtcttattca actatgtgta tgaaaataag gaagcttttg aagcaggcga
    36961 taagccagta gcgaaggatg ttagcttgaa taaccaagcc caaactgtca gttgtacagt
    37021 ggaacaccat gtttctatcc aaacaaaggc ccacctagaa aacggttctc agacctttac
    37081 tcatggtgat gtggtggata tgtttgatga tgtgtcgatt actcatgatg tactggatgg
    37141 ctcaaaagaa gctttcgaaa caattctgta tgctttatta ccggatggta caaacaaaga
    37201 aatttggaaa tctggcaaga ttgattatga agtaaatgat aaagaattca ccaaaactgt
    37261 gcttgcgaaa aaagtagata ccggaaaata tccagaagga accaagttta cttttgcgga
    37321 aatcaattac gataaagatg ggaatatcaa tggaaaacac aatgaagatt tgaaagaaaa
    37381 atctcaaacc ttaacaccaa aagaagttcc aaccatacta agtacgccga aacaaccgga
    37441 aacaccaact gttccaagtg gctctcaaga atctagtccc acagtgaaga ccttccctca
    37501 aaccggggag aaaaattcca atgttttact gttcattggt tttactttga tttttgcgac
    37561 ggcgggctat tatttctgga atcgccggaa ctaaggtgat gagatgatac aagtaaatcc
    37621 taagaaccaa cgtgcgcgac cgcctcctaa aaaagtagtc gcgctatttt tgtatccaaa
    37681 actaacaatg aaaagaggaa aagaagatgg aattgaaatt tgtcgtgcca gatatggctg
    37741 aaacctttgg aaagttaagt tatgccggtg aaggagaaat tataacagaa ggatatggac
    37801 ggaatactac ggtgattggt cgcagttatc atttgtattc cagtaagcaa cgggccgatg
    37861 atattgaggt ggtagtagct gcggaagccg gtgaaaagga ttttgatcaa gaccaaccgc
    37921 taaaagccgt gaatccccat ttggttgcca aaggctatga gattgagaat cgtgggttta
    37981 ctgattacgt gttgtatgtt gatgatttag tgaaagcata ggaggaaaag aagatgagat
    38041 taacagaagg tattgtcgta gattcaggtt taacatttgg gaagttgcgt ttttccgcat
    38101 tacgccgaga agtacggaag caaaatgagg atggaacaat tggtaatgag gtaaaagaac
    38161 gaacctataa tttaaaatcc tctgcacaag ggcgaatgat tcaagttagt atcccagcta
    38221 atgtaccctt acgcgagttt gcttatgatg cagaggtaga gttggtcaat ccaatcgtgg
    38281 ataccgttgc caattatgtt tttcgagaag gaactaccgt taattggttc attaaggcgg
    38341 atgatttagt cttgaagcgg cagccgaatc aaggaaatcc tacgaaccaa aatgaaagaa
    38401 agaaataggt gaagattatg gaaattaatg ttgaacatat tttagattgt ctcgatcaat
    38461 atggaaaagg cgagctgact gaggaacaac tcgttaaagc attaacctct gatgaaaaaa
    38521 tgtttctgat tatacatcaa ggtttacttg gagaaaatag cgacagtgaa gaagactctg
    38581 atgtactaga ctggttagga gaagaagagt ctttctttat agttgtcgaa gtaaatgaaa
    38641 acttatgtcg gcaggcagaa tccattttgg aagaaattgg tgtggagatg ccggatgcta
    38701 ttgaaggctt tttaaatcaa ttggttgaaa cgaaacaact cccctcctcg tagcagtaaa
    38761 tgactagcat aactttttga aattgcctaa tgaaatccta ttgggctttt tcgattggag
    38821 gtggaaacca tcaaaatgta caaaggacat cgcatacgcg caggagatca gcacttggtt
    38881 tatcactttg ttcttggctg gctactcgct ctgttcatcg gttggatgag cgtcttttat
    38941 tttcaagagt ttagacaatt tgatatttct aagctatcac tttctactat tgaaatcgtt
    39001 tggtctataa aagatttagt atgtctgctt ggaagtctcg ctttttcagg agctatgata
    39061 ttgctttata ttcacttttt tcttgatcat tggcgaagct tgtggcatcg acaaaagcta
    39121 gctcggatga ttctagagaa ccattggtat gaagtgaaac aaactcaaag tgaaggcttt
    39181 ttcaaagatt tgaatagtag ccgaaccaga gagactatca gttacttccc caaaatctat
    39241 tatcgaatga aagatggatt actgtctatt cgcgttcaaa tttcacttgg gaaataccaa
    39301 gatcagcttt taaaattgga aaagaagtta gaaagcggac tgtattgtga gttagtagaa
    39361 aaggaactca aagactccta tgtggagtat actttactgt atgatatgat cgccaatcga
    39421 attgggatag acgaagtagt ggcagaaaat gggactctgc gattgatgaa aaatcaagta
    39481 tgggcttatg attctttacc ccatatgtta atcgctggtg gcacgggtgg agggaagacc
    39541 tatttcctcc tcaccattat cgaagcacta ttgaagtcag atgcagaatt gtttatcctc
    39601 gatcccaaaa atgcagattt agctgattta ggtacggtga tgcctcatgt ttattcacaa
    39661 aaggaagaaa tttctgcttg tgtggaagat ttctatgaac gtatgatggc tcgtagcaag
    39721 gcaatgaaag aaatgtctaa ctacaaaaca ggagagaatt atgcctatct tggacttcca
    39781 cccaactttt taatctttga tgaatacgtg gcttatatgg agatgttaac gacgaaagaa
    39841 agtgcggtga ttttgaataa gctcaaacaa atcgtgatgt tgggtcgtca atctggtttc
    39901 tttctgattt tggcttgcca aaggccggat gcgaaatatt taggagacgg aatccgcgat
    39961 caatgtgcga catgtttcta actgaaaagg ttaggcactt aaaagaagaa aacataagtt
    40021 tccttttaag gagaactgat attttgagca gtggaatgat ggggtaacgc cctgaaacgc
    40081 tcccttaata ctccgactgg ctgtttaaca tatagctgtt agatggtcag aagctcggtg
    40141 aagtcggctg aaagttaccg taaatatgat aaattcatat acgaaagttg ttcagaggtg
    40201 aatgatgtaa cctataggtc ggaggtctat aaaatactca cggtgagaat gtaagtaata
    40261 ctactgacga acttgcgaat gaaagaatct atacacggac tgtatagaaa tgtacaggat
    40321 tctaccgtat caacggaaca gaatgtatgt tgtggtttag taagattatt ggttatgaac
    40381 aaccatgcaa tgttacaggc attggaaata tacaggttca aagcaagcac ctaaaagtat
    40441 atataaagat aaaaatatcg aaacatggga agctggcaat gtggaggaaa ctatattcca
    40501 ttgaaacatt tgtgacgaag aataataagc acatttatgc cagtgaaagt ggtggcacag
    40561 taccaatgag gatatagaaa tatatcggag gaatagccac tagtcaaaag aaatcgttaa
    40621 ttaaatgatg tatttcatag aatcgagtaa gacaaaataa acactacaac ctaagtaaac
    40681 aaaggtagtg gtgctatgaa taaagtaaaa acaaataaaa cacttcgtca tagtgaatac
    40741 tatggcatga cacaaatatt tgatgaactg tatgtaaaaa gtgataaaaa agaagttttt
    40801 acagagctca tgacaattat tatgagtgat gaaaatattc gactagcata cagaaatatc
    40861 aagaataaca agggcagtta tacagcgagt accgataaga aaagtattcg agacattaaa
    40921 acactgtcag aagatacctt tctaactgag gttaaagttc tttttcgctg ttacaaacca
    40981 cgagcagtta gacgtgtaga aattcccaaa ccaaatggaa aaacacgccc tctgggcatt
    41041 ccatcaatct gggataggtt aatacaacag tgtattttac aagtcttaga accgatatgc
    41101 gaagctaaat ttcacaatta tagttatgga tttcgaccaa accgttccac acatcatgcg
    41161 attgctacct gcaatcgact ggcaaatagg gggaagctac actttgtagt agatgttgat
    41221 attaaaggat tctttgataa tgtcaatcac tctaaacttt tgcgtcaaat gtggacgatt
    41281 ggaatccgtg ataaatggct attaggtatt attcgatcca tgctaaatgc tcctatagtc
    41341 ctaccaaccg gtaaagtcga acaccctaaa aaaggtgtac ctcaaggtgg tgtcctatcc
    41401 cctttactat ctaatatcat attgaatgaa ttagattggt gggttgcttc acaatggcaa
    41461 aataccacga ttgagacaga aaagattatg gataggcgag ataaaggcaa aggcattgat
    41521 aagactaatc agtatcgggc attaagacga acaaacttaa aagaaatata cattgttcgg
    41581 tatgctgacg acttcaaaat cttttgtaga aaacgaagtc atgcagataa aatatttcac
    41641 gcaacgaaaa tgtggcttaa agaacggtta tcacttgaaa ttagcgaaga aaaatccaaa
    41701 attgtcaatc tcaaaaaagg taaatcagaa tatctaggat ttgaattaaa gctaatcaag
    41761 aaaggtaaaa aatacgtagt cgaaagtcat atgagcaaaa aatcaatgac aagagttaaa
    41821 atccaattaa agaaacaatt acgtaaagtt gccagaccga agaatcattg cgaacaagca
    41881 aaagaaattg gattatataa ttctatggtg attggaatcc atcaatatta tggaattgca
    41941 acctgtgtaa atctcgattg cagtaaaata gcctatacac taaaaccgtt catcaatcac
    42001 aaactgccga caaagaaaca tggaaagata ttaaatacat ttatcaaatc gaagtatgga
    42061 aaaagtaaag aaatgcgatg gttgaataat gtgccaattg taccgctcaa ctatgttcag
    42121 tttagacttg ctgtccccct atcagaggga acttgcaagt acactgaaag tggacgaagc
    42181 aagatacaca gtaaattaaa attagaccta gcccttttac ttcacatgat gagaaatagt
    42241 aattatgaaa gaagtattga atttgtggat aacagaatat ctaaatattc tgctcaaaag
    42301 ggaaaatgtg ctatcacggg taaatttctc gaatacgaag aaatccattg ccaccatatc
    42361 gtaccagtaa aacaacaggg tacagacaaa tacagcaacc tcattattat tcacaaaaat
    42421 gttcacgcat tagtccatgc tatcgaagaa aaaatcatac acaaatacct gaatgtatta
    42481 aatctaacca atgaacaaat agaaaaacta aatgaattac gtgtcaaagc tggtaatagt
    42541 gttttaacag tttaacttga aaaatatatc atacttaaca ttcttttgat ggaacgccgt
    42601 atgaagtgaa agtttcatgt acggtgtgaa gcgggggaaa atctggagat gacatcaaag
    42661 gattacctat cgctattcaa ctttcgcgtg gctttgggtc gtatgagtga actcggatat
    42721 tccatgatgt ttggggaggt cgaaaaaaac ttctttatga agcacatcaa aggtcgaggt
    42781 tatgtggata caggtggcag tgtgattagt gagttttata cgccacttgt accaaaaggg
    42841 tatgatttct tagattcaat tagtaaatta gaaaagatga atgattgagt gatcttcaat
    42901 actgacttta tacttcaagt aacgtataat acttttatat agtaggaaga aaggggataa
    42961 ttaatatgga aagtaatgat tcttatttag agtatgcaaa agcaaataag gataagttta
    43021 tttcttatat tatcaaaggg aaatcccccg aagttggacg agacgcagtt tttatggcag
    43081 ggagtcctgg ggctggaaaa tcagaggttg ccattggatt agctgagaat tatgataacc
    43141 atgtgattat tgatgcggat tactttcgca cccagtttcc tgattataat ggaaaaaact
    43201 ctagtctctt tcaaaaagca tcttcttggt tggtagagca ggcatttaga tatgtcttag
    43261 atcatggcta ttcttttatt ctggatgcaa cttttgcaat attaagtgct gaaaaaaata
    43321 tctcgcgggc gttgaaaaaa cattttcgag tgacgatttt ctatgtttat caagacccta
    43381 aaatcgcttg gaaatttaca caagaacgtg aaattgcaga gggacgtcat gtgccgaaaa
    43441 aggcatttat taacgctttt ttcaaatcaa gagagaacat tgcaaaagta aagagtaaac
    43501 atccagatgt attgcttcat atcataataa aagattatac gaataatatt tccgaagttc
    43561 attatgctac ggataatcta gaattatttt taccactagg gtatagtagg aaagatttgg
    43621 aggttcaatt acatgactga gaaagaacaa gtaacgaaaa ttgtaaagaa atacaataag
    43681 agcattgcgg atttatcgga aaatgctact gcaaaagagt tcaaaacggt gattaaatat
    43741 gtagccgatc aggccaatga aaaacaacgg aaattagttg gattaaataa aaaatagtag
    43801 gacaaattaa taatgtatga gccaagagca aaatgttctt ggcttttttc tttgcaatga
    43861 atgaggtaat gtatgagatt tttagattta ttcgccggta ttggtggctt tcgtttaggt
    43921 atggaacagg cgagccatca ttgtatcggt ttttgtgaaa ttgataaatt tgctagacgt
    43981 agttataaag caattcatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta
    44041 tcagatgagt ttattcaatc cctcggacca gtggacatcc tttgcggcgg atttccgtgc
    44101 caagcttttt caattgcggg aaagcggcaa agattttccg atactcgagg tactttattc
    44161 tttgaaatcg ctcggttcac cactcttctc cgacctaagc ttttattcct tgagaacgtc
    44221 cgaggattgc tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat
    44281 ggattggggt atgacgtgga gtggtaggtg cttaactcaa aggcctacat tccccaaagc
    44341 cgggagcgga tattccttat cggacattct cgagacgcat gtaccgaaca agtatttcct
    44401 atcattggac cgtcttcaac atctgatcaa aacatcagaa atttggtaaa tatcaatcct
    44461 tccaatcgag gaatgggtgg acaagtctat ggttcagatg gcgtagcgcc aaccttaaca
    44521 ggtgatgaag gaatcaaaat cgcactgcca gcaagggatg gaatctctgt agcaggacta
    44581 ttaccgggaa agtttgaaca aggtaatcga gtttatgaag ttactggaac cgcccccacc
    44641 ttgtcaacga aacaaggtgg tacaaaaatc atgattcgaa aaaatgaaac agaatatcaa
    44701 gaggtgaagc cagaagacag tgtgaatctg gcttttccta attccacaag tcgccgaggt
    44761 cgtctgggaa aacagagtgt tcatacctta ctaacaggag accaacaagc gatcgtcacg
    44821 gatcagtatc aaattcggaa gctaacacct agagagtgtt ggcgacttca gggctttccc
    44881 gattgggctt ttgatcgaga ttctcaagtc aattcagata gtcagttgta taagcaagcc
    44941 ggaaactctg taacagttcc ggtcattttt gatattgcca gaagattaaa agagttggat
    45001 gtacatggtt tatgatgagt tgattggaga aatttattgg gtgattggaa agattcagtc
    45061 agacccagaa ttagaagaac aattgcgacg gttaaaattt gatattcgca agaatggtgt
    45121 caaagtcgct ggcgatccat atttaatgaa tgaggagacg gatgctcgaa ttgaaataaa
    45181 tcaagtaatt gcagaatttg agcggattgc ggaccaagca aaagaaccag atattcgtca
    45241 atacttgtta gaaatgaaag cagagttgga agtaaatggt attacagatg agtaacctaa
    45301 accaacacaa ctgactgtta ggcgctgacc gcggaggatt cgcgaagcag aaatcctccg
    45361 tggtgctgtg cctgttcatg gacggcgtta gccgaccatg atgtctcacc cccgtatcta
    45421 acaggggggt acaaaaacac aaaaaataag taagcaacca ttaagaatgc ttgttgtatc
    45481 aaagatagta agtaatttgt ataggtgtta actcatttga aaaagttaac accttgaatt
    45541 tggaggtgag aacttggcac aacggaattt agattatcgg ctattaaaag accgacgcaa
    45601 cgaatacggc gtttcacaaa ataaattggc tacggcttgt ggacttagtc ggccgtactt
    45661 aaatcagata gaaaatggtg gcgtaaccgc atctataaaa accatgagga aaatttttga
    45721 ccaactggaa agctttaatc ccgatttgcc tttgacgtta ctgtttgact atgtaaggat
    45781 tcgctttccc acaacggatg cacgaaaaat tattcaagaa attctccatt tgaaatttga
    45841 gtatatgctc catgaagatt atgcctttta ctcctatcaa gaacagtatg tcatgggtga
    45901 tattgtggtg atgttgtctc atgaagaaga taaaggtgtg cttttagaat taaaggggcg
    45961 aggttgccga caatttgaaa cttttttact cgctcaaaag cgtagctggt acgacttttt
    46021 cgaggattgt ctaaaggccg gtggcgtgat gaaacgcttg gacttggcaa tcaatgatcg
    46081 agtggggcta ttggatatac ctgagctaac gaaaaaatgt cagaaggaag aatgtatctc
    46141 cttgtttcgt acctttaaaa gttatcgttc cggggaactt ttaaaggctg atgaaaagga
    46201 cggcatgggc aataccttat atatcggcag tctcaagagt gaggtttatt tctgtttgta
    46261 tgaaaaagac tatgaacaat atatcaagtt agggattcca ttagaccaaa ctgagacgaa
    46321 aaatcgtttt gagattcgac tgaaaaatga ccgtgctttt catgcgattc aagatttgtt
    46381 aaaaggtcgt agcattgaga gcaccacgtt ttccattatc aatcgttact tgcgatttgc
    46441 ggataaagta gaaggtaaaa ggcgaaccaa ctggcccttg aatgaacaat gggggcgttt
    46501 tattggacgg aatcggaaag agattcaatt aacttccgag ccaaaaccgt ataccattga
    46561 acgaaccttg aattggctag gccgacaagt cgcccctacg tggaaaatgg ctaaggagtt
    46621 ggatcgattg aatcggacaa cctatatcca ggatatggta cagaatgcac gcttatcaga
    46681 ccgacataaa aagattttgg agcaacaaag tatggcaatt gaaaatttga ttatatgaaa
    46741 agaggaatcg tcatgggaat tgtaaaagat atttttttgg tatttggtgg cagtatgctt
    46801 ggtgttactg tgatgtgctt gatgaatgca agtgcagcag cggatcgagc aatggaaaag
    46861 aaaggaaagt gacaagatga atttcggaca aaattt
//
LOCUS       DACEUF010000010        44603 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.011, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000010 DACEUF010000000
VERSION     DACEUF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.011"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            249..815
                     /gene="amaP"
                     /locus_tag="IXG20_000584"
     CDS             249..815
                     /gene="amaP"
                     /locus_tag="IXG20_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1608800.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            828..1016
                     /locus_tag="IXG20_000585"
     CDS             828..1016
                     /locus_tag="IXG20_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1608801.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1029..1586
                     /locus_tag="IXG20_000586"
     CDS             1029..1586
                     /locus_tag="IXG20_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1608802.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1618..1860
                     /locus_tag="IXG20_000587"
     CDS             1618..1860
                     /locus_tag="IXG20_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1608803.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2201..3415
                     /locus_tag="IXG20_000588"
     CDS             2201..3415
                     /locus_tag="IXG20_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1608804.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            3446..4873
                     /locus_tag="IXG20_000589"
     CDS             3446..4873
                     /locus_tag="IXG20_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1608805.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            4896..5822
                     /gene="glsA"
                     /locus_tag="IXG20_000590"
     CDS             4896..5822
                     /gene="glsA"
                     /locus_tag="IXG20_000590"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ1608806.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6030..7490
                     /locus_tag="IXG20_000591"
     CDS             6030..7490
                     /locus_tag="IXG20_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608807.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            7711..9474
                     /locus_tag="IXG20_000592"
     CDS             7711..9474
                     /locus_tag="IXG20_000592"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ1608808.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            9558..10016
                     /locus_tag="IXG20_000593"
     CDS             9558..10016
                     /locus_tag="IXG20_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ1608809.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10125..10808
                     /locus_tag="IXG20_000594"
     CDS             10125..10808
                     /locus_tag="IXG20_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1608810.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RSNTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            10955..11569
                     /locus_tag="IXG20_000595"
     CDS             10955..11569
                     /locus_tag="IXG20_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ1608811.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            11585..11791
                     /locus_tag="IXG20_000596"
     CDS             11585..11791
                     /locus_tag="IXG20_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608812.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            12308..13471
                     /locus_tag="IXG20_000597"
     CDS             12308..13471
                     /locus_tag="IXG20_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1608813.1"
                     /translation="MKKRKKIDWWILGPYLTLSMIGLLEVYSASSYRLLQADENTKSL
                     LLRQLIFIFLSWSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGA
                     QRWISLFGIQFQPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPK
                     IAGALMILSIAWVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNH
                     AYERIATLRDSFIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIF
                     SIITEELGLIGALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVG
                     SIAGLMPMTGVPLPFVSYGGTSYLILSLGIGITLNISSKIQAEELPLYRPEKQ"
     gene            13563..14318
                     /locus_tag="IXG20_000598"
     CDS             13563..14318
                     /locus_tag="IXG20_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1608814.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSVPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            14376..16415
                     /locus_tag="IXG20_000599"
     CDS             14376..16415
                     /locus_tag="IXG20_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ1608815.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEQPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQVTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFANNGTLVY
                     PKLIADKETKDKKNVIGETALQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGKENSFLFAFNPDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(16524..17675)
                     /locus_tag="IXG20_000600"
     CDS             complement(16524..17675)
                     /locus_tag="IXG20_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1608816.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(17829..18359)
                     /locus_tag="IXG20_000601"
     CDS             complement(17829..18359)
                     /locus_tag="IXG20_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608817.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(18458..18808)
                     /locus_tag="IXG20_000602"
     CDS             complement(18458..18808)
                     /locus_tag="IXG20_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ1608818.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(18965..20494)
                     /gene="ahpF"
                     /locus_tag="IXG20_000603"
     CDS             complement(18965..20494)
                     /gene="ahpF"
                     /locus_tag="IXG20_000603"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ1608819.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(20504..21067)
                     /gene="ahpC"
                     /locus_tag="IXG20_000604"
     CDS             complement(20504..21067)
                     /gene="ahpC"
                     /locus_tag="IXG20_000604"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ1608820.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(21211..21873)
                     /locus_tag="IXG20_000605"
     CDS             complement(21211..21873)
                     /locus_tag="IXG20_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1608821.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            22057..23703
                     /locus_tag="IXG20_000606"
     CDS             22057..23703
                     /locus_tag="IXG20_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1608822.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            23694..24776
                     /gene="uxuA"
                     /locus_tag="IXG20_000607"
     CDS             23694..24776
                     /gene="uxuA"
                     /locus_tag="IXG20_000607"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1608823.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(24884..26437)
                     /locus_tag="IXG20_000608"
     CDS             complement(24884..26437)
                     /locus_tag="IXG20_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1608824.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            26585..27874
                     /locus_tag="IXG20_000609"
     CDS             26585..27874
                     /locus_tag="IXG20_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1608825.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(28000..29079)
                     /locus_tag="IXG20_000610"
     CDS             complement(28000..29079)
                     /locus_tag="IXG20_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1608826.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(29188..29628)
                     /locus_tag="IXG20_000611"
     CDS             complement(29188..29628)
                     /locus_tag="IXG20_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1608827.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(29685..30095)
                     /locus_tag="IXG20_000612"
     CDS             complement(29685..30095)
                     /locus_tag="IXG20_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1608828.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(30276..30710)
                     /locus_tag="IXG20_000613"
     CDS             complement(30276..30710)
                     /locus_tag="IXG20_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1608829.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(30810..31658)
                     /locus_tag="IXG20_000614"
     CDS             complement(30810..31658)
                     /locus_tag="IXG20_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1608830.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            32047..33813
                     /locus_tag="IXG20_000615"
     CDS             32047..33813
                     /locus_tag="IXG20_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ1608831.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            33915..35174
                     /locus_tag="IXG20_000616"
     CDS             33915..35174
                     /locus_tag="IXG20_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1608832.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            35321..36622
                     /locus_tag="IXG20_000617"
     CDS             35321..36622
                     /locus_tag="IXG20_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1608833.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            36625..37476
                     /locus_tag="IXG20_000618"
     CDS             36625..37476
                     /locus_tag="IXG20_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1608834.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            37489..38304
                     /locus_tag="IXG20_000619"
     CDS             37489..38304
                     /locus_tag="IXG20_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ1608835.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            38455..39621
                     /locus_tag="IXG20_000620"
     CDS             38455..39621
                     /locus_tag="IXG20_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1608836.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            39851..40498
                     /locus_tag="IXG20_000621"
     CDS             39851..40498
                     /locus_tag="IXG20_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ1608837.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            40582..41526
                     /locus_tag="IXG20_000622"
     CDS             40582..41526
                     /locus_tag="IXG20_000622"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ1608838.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            41661..43112
                     /locus_tag="IXG20_000623"
     CDS             41661..43112
                     /locus_tag="IXG20_000623"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ1608839.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAVIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            43133..43969
                     /locus_tag="IXG20_000624"
     CDS             43133..43969
                     /locus_tag="IXG20_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1608840.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            43969..>44603
                     /locus_tag="IXG20_000625"
     CDS             43969..>44603
                     /locus_tag="IXG20_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1608841.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLE"
BASE COUNT        14930 a         7832 c         8710 g        13131 t
ORIGIN      
        1 actgagcata atctttgcaa tttttaatct tgaataatat cctttaagta tagccagcaa
       61 tcaaaagtaa atagagaata agtaagcaat tgttatttca gtatcagact ggcaataaaa
      121 cgccgttatg atccagcaat gtttggtagc gctttattta ctgatgctat gctaactgtt
      181 cgctatctat actgtgattg tcggtagagt tatttacagg gtaaaaataa gtttatagga
      241 ggtggaaaat gaataagggg ttaaaaataa tcggtgttat catgagttta ctgctacttt
      301 cagtatttct agcgattgcg ttgatcaatg cgccgtatat tatgcctgaa caattggaac
      361 gatttcgctt ctttacttta acgaattact acatgcaaca atatattttc tgggcagctg
      421 tagtatttgc cgttcttgct attatcatct tgctagtcgt tcttttctat ccaaagtcaa
      481 gaggaacttt cgttatgaaa cgggaagatg gcaaactaac aatcgacaaa aaagcgatcg
      541 aagggttggt acgttcccat ttacacgaag aagagttcat tcattcacct aaagtacgga
      601 tacgttcaac gaaaaatcga atccatatcc atgtcaacgg agacttgaaa cgaacttctt
      661 cattagtagg gaaaacaggt gtattgatgc aagatatcga agaagaagta actaaagtac
      721 tcggaacaaa agaaacggtc aaagtggcag tcacatacag cggttatgaa gaacaagaag
      781 atacaagaga ctacaagcac tctcgagtgg aataggaggg ccaaataatg aaagaactat
      841 tttcagcata caagctccct attatctttg ggggtcttgg actactgcta gctgttttat
      901 ggttaacgat tggatttttc aagacattgt tgatcataat catgacagca attggcgcag
      961 gaatcggttt ttatttgaaa cagtcaggaa tagttgatca attttttaaa tcataggagg
     1021 aaacaaagat ggaaaacaaa aacatgaata caacaaatgg atcagttttg gcaaatgaaa
     1081 aaggagaaaa ccgcatgatg aatggtgaat tgacttatga agacaaagtc atccaaaaaa
     1141 tcatcggtta tgcattgaaa gatgtagatg gtctattgac attagatggc ggatttttct
     1201 caaatatggc tgaaaaatta gtcaacacag ataacgtaac agcaggtatc aatacagaag
     1261 taggtaagaa acaagtagct gtggatctag atgtgatcgt agaatacggt aaagacatcg
     1321 aagatattta caaccaaatc aaagaattga tcagtactga agtaaacaat atgacacatt
     1381 tagatgttat cgaagtgaat gtgaatgttg cagacatcaa aacacaagaa gaataccaaa
     1441 aagatcaaga aacagtacaa gacaaagtaa cagaagcagc aaaatccaca ggacaatttg
     1501 cttcacgcca aacagataaa gcaaaatctg ctgtaggcaa aggtgcacaa aaagtcaaag
     1561 aaaataacga accacgtgta caataagaag taagaagtca gttaggagga atttaaaatg
     1621 ggatttattt gggcattaat cgtcggcggt atcattggag caattgcagg agcaatcacg
     1681 aataaaggat catcaatggg gatcatcgca aacatcattg ctggtttagt aggttcagca
     1741 attggacaag ctttactagg aacttgggga ccaacaattg caggaatggc tatcattcca
     1801 tcattgatcg gtgcaatcat tttagtatta gtcgtttcat tctttgcacg taaattataa
     1861 gcaaattgaa gaaaaactta tgttgaaatg aagaacaaat ttgagaaccg tagcgacact
     1921 cgctgcggtt cttttttata tattttatat gaagtattct ttattcctta tgaaaaaata
     1981 tctttaaaaa taaaaaaaga cgaaaataaa cggttttcta taaaaagaag accgtttatt
     2041 ttttgtcttt atttgtgtga aaaacgaaaa agagcacaac acatcaagct tttattaatt
     2101 gtctgtcttt tataaatgca agtattaatt caaaaaaaca gaagaaaaaa acaactacac
     2161 cttcttttat actcgtaatc gtagaaagga ggcgaaaaaa atgaatacat tacctatcaa
     2221 tacaggagat acagcattca tgattttatg tacagcaatg gtttgcctga tgactcctgg
     2281 tctagctttt ttttacggcg gattagcacg gaagaataat atactaacga tcatgtctca
     2341 atcttttctc tccgttggga taacaacgat tatgtgggta tttggtggat tcggattggc
     2401 ctttggacgg gatatcggcg gtgttattgg aaacccggca gatttctttt tgatgagaca
     2461 tgttacaagc ttccccaaca tgtttcatgg tgcaacgatt ccatttttaa tgttctttct
     2521 ttaccaactg atgtttatcg tgattaccgt tcctttgatg acaggagctt ttgcagggag
     2581 attgaattta aaaggctata ttatacttgt tattttttgg aatctgctga tttatttccc
     2641 tgtttgccac tggatatggg gtggcggatt tttggatcag atgggattta gagatttcgc
     2701 tggtggagca gtcatccaca cgacagctgg attcggctct ttagcatgta tcctcacact
     2761 tggtcaaaga aagattcctt taggagaaag aagtcagcat agcagtctta tggcagctgc
     2821 tatcggtaca ggactgcttt ggtttggctg gttcggtttt aattccggtg gtgcacttcg
     2881 agcagatgaa caagcagtca atgcatttgg ttcaacattc atcgctcttg catttgcgat
     2941 gattacttgg ctgatcattg caaaagtaaa aggaaatggc tttgattttg tcgacgtact
     3001 aacaggatcc gttgctggat tggcagccat tacgccttgt gccggttatg tagaagcaaa
     3061 aagtgccatg ctgattggga tcattgcggg tatcgtctgt catgctgctg ttgattttcg
     3121 taaaaagaaa caatgggatg atgcacttga tgtgtggggt gttcatggca tgggcggatt
     3181 cacaggtacg attctgatag ggatctttgc atctaacagc catttactaa cgaatccaag
     3241 cagctggtac ttcttaggga ttcaagtgat tggtgtcatc attactgccg tgtatgccta
     3301 tgtgctgaca acagtgattt taaaatcggc gatgcacttt acaacgatca ctactacaaa
     3361 agaagaacaa gaagaaggat tggacttttt cttctttggc aacaaactca attgaaacaa
     3421 attcaaatag aaaagaggaa gattcatgca acaaagcaaa acgaaaattc ctgcaaaaaa
     3481 attaacgttc tttggctttt tggctatgac catctcaatg gtcgtttcat tatatgaata
     3541 tcccacattc gcgacctcag gcttcagctt agtatttttc ttgttattag gcggtttgct
     3601 atggtttatc cctgtggcat tgtgcgcagc agagatggca acggtgaaag gatgggaaaa
     3661 aggcggtgtc tatacttggg tcagtcgcac attgggaaaa cgattcggtt ttgccgcaat
     3721 ctttttccaa tggtttgaga taacagttgg ctacttgacc atgctgtatt tcttaactgg
     3781 tgcattatcc tatgcaacgg gtattggtgc tatacaaaac aataaatatc tgaaattggc
     3841 aatcctattg attattttct gggctatttt actctcacag ttaaaaggaa ccaaatacac
     3901 ttcattgatt gctcggatcg gttttgtagc tggtatcttg ttgccagcat tagtcttgtt
     3961 tggcttaggg atccattatg tcgcttcagg cgctccacta caaacgatac tgtctatgaa
     4021 aacattgatt cctgattttt cgaaattgcc tacgctggtt gtttttgttt cctttatttt
     4081 agcatatatg ggcgtagaaa catctgccag tcatgcaaat gaaatggaaa accctaaaaa
     4141 ggattatcca ttagcaatgt ttgtattggt tattctggca atcattttag atacttttgg
     4201 cggattgacc gtcgctacta cagtacctca acatggtttg tctcttaata caggggtgat
     4261 tcaatcgtta gaattcctat taagacacat tgatccaaat cttgcatgga ttgcgaaaat
     4321 tcttgcgatt cttgtttgtt taggtgtcat cggagaaatt gcttcttggg taacctctcc
     4381 ttcaaaagca cttcacgtag cagcagaaga aggattactt cctgaatatt ttgcaaaaga
     4441 aaacacacac ggtgttcctg ctcatttgat gatcgcaaat gggatcgttg caacaatctg
     4501 ggcagccgtc tttacattaa gcggcggggg gaataatctt tcatttcttg cagcgatgtc
     4561 attgactgtt gtaatctacc tgatgttgta ctttatgttt ttcgcgggct atttgaagct
     4621 ggtttttaaa gatgacagtc ttgaaagagc ctatcaggtt ccaggcggaa aagttgtaaa
     4681 agtaatcgtt gcttgtttag gtcttttgac ttcacttttt gctttttgta tctcatttgt
     4741 tccacctgct agtatcgcag ctggacagca tgggatttat gaaacgattc tgattgtttc
     4801 ttatcttatc gtcttagtta ttccatttac gatctacgca tgccgtcata aattccagca
     4861 tgcagaaaaa taaaaaatga aagctggtta ttcaaatgga agtaaaagaa ttagaacaat
     4921 tgattggaaa aaatttccac tattatcaaa caggacaagt ggcggattac attccagcat
     4981 tagctgaagt ggatcccaaa caattaggta tggcgattta tgatttggat acaaaacaac
     5041 tgattgaggg aggagacgcc gatgtgcgct ttgccatcga aagcatgtca aaagttcccg
     5101 tattgttatt agcaatcaaa gacaacggaa tagaaaaagt ctttcaaaca atcaacacgg
     5161 aaccaaccgg ttttgccttc aattctatca tgaatatgga aatcaatcat cgcaaacatc
     5221 caatgaatcc ttttgttaat gcaggagcga ttgcaacaac ctcgttgatt gccggtaaag
     5281 atgcagatga aaaatttgag cgaatcttgt catttatgaa agagatctgt gatgatccta
     5341 atatcacatt gaatgaagaa atctatcatt ctgaatcaag aacaggtgat atcaatcgct
     5401 ctcttgctta ctatatgaaa ggtaatcaaa tgatcgaagg agacgtaccg gagatactcg
     5461 atgcttattt cagacaatgc tccctcaatg tcaccgctac aggcatagca aaattagctg
     5521 ctgtgctggc aaataaagga attgctcctt ggaatggcaa acaaatcatt actgaagaaa
     5581 gtgctacaat cgtcaaatca attatgacaa ctgctggttt gtatgatgaa tcaggagagt
     5641 tttccgtcca tgttggtgta cctgcaaaaa gcggtgtagg cggcgggttg atggcagctg
     5701 tgccaaatcg ttacggcata ggtgtcttca gcccggcact agatccattt ggtaacagcg
     5761 cagctggtat ccaattgttg aaagatgtag tcaaagaact agacgctgac atctttgaat
     5821 aaccattctt gaaagaggct ggggtaaaag tccaaacggc tcaaagctac ctacaatgag
     5881 gctgaaattg tgagcttgtt tgacttttgt cccagtttct ttttttattg gtttttgaaa
     5941 gggcttttct aaataaaaag gaaacaaaaa tacgatttac agatatgatt aaataaaatc
     6001 ataagaaaaa ctggaggcgt gtatcaggaa tgctggaaaa ttacatcgag cgaaatatat
     6061 ttagaaaagt gtatctgtgt gaacaattat ttgaattcca agaaatagac atcgaacaaa
     6121 cagctatttc tctaagggtg actacaccaa cgatccttca cgatctagaa agtctagccg
     6181 agtgtctaga atattgtata aaagaacaag taagagagaa gcataagtat aaactcgtat
     6241 tcaaacatgg tattgctctt tctgagctca cacagtttct gtacggtcaa tcttattttt
     6301 taaagttttt aagttattat tttaatggta tattcacgag tacagaactg gctgatcttg
     6361 aatttatttc tttgagcaaa gtatatacaa tcaaaaaaat cgtattggat ttttttaaag
     6421 aaaatgatta tttgaaaaat aaagaaatca ttatcccaga atttgactcg cggaacatct
     6481 tgctagcttt agtacgttat gtcaattggg aaggctatga aaataaaaat ccgtatgtag
     6541 aagaagcttg taacgagttg attgattatg tagagactca tttttttaaa agaagatatt
     6601 cggaagatga aaagtccttt atcatacgag gaatagagat cgcaatcgga agaaaggcat
     6661 ttccccttgg actgaaagaa gaagataaga aaagcgcaga aacgaagccg ttatttcaat
     6721 tagtctgtca aggattggaa gagcaaaaag gaattctaga cttacaagaa gaagatgtct
     6781 attatatttt ttctttgttc aacagtagaa attataccaa tgagaacatg gagctgttaa
     6841 gaaaagattt cgaagttgtt tataaaaatt ttgttctcaa tgacctatca ctgaatgaat
     6901 tgattttaca gattattagt atcgctgaaa tcgacgatgc agaaaatttt attttcaagc
     6961 aatccttttt acaatttgtc cgtacgttgt gggcagatgg acaagtattt ttaccggaaa
     7021 ggatctatct tctatcagaa aaagaacaac ttctgtacgg aaaaattgta gaaatattgg
     7081 aagagtggaa aaagaaaaat caaatccaac taagatggaa tgaaaatttc attcgaaaat
     7141 tcgtcaaaag tctttctttg atcaacagtg aacaagcggg gagacaggaa atagaaattt
     7201 ttgttgtttc agaaagttca gtcaaacaat ttttttatcg aacccaattt catttgtatg
     7261 cggaagaagg catcgctgaa ataaatccta ttttatttcg ttcattagaa gaattgcctg
     7321 atgaatgtct atgtgcgaaa aaaagagtgg ttttgtgcga tgctgctagc tatcaaaatg
     7381 gattagaaac tgaaaagaca aagatctatc caatctcatt aaaaggtgca aacatccatt
     7441 tgtatcatat tgctcaggaa cttcacctat taaaaaaaca tatttcttga tgagacctta
     7501 attaggagac cttctacaaa gctttttaat tgtctgtaga aggtttttat ttgagtcctg
     7561 attcgtgcga agatctactt atataacaag ctttctctga aattttttta aaatgctatt
     7621 gacttagagt caactctaag tagtagaata gataaagtgt aacaaatcta ttttcattaa
     7681 aagaagatag tacggaggag agaatgtatt atgagtacag tattacatgt attggatgca
     7741 atcaatagcc ccgcagatgt caaaaaatta tctgttgagg aaatgaaaca gttggcagaa
     7801 gagatgcgcc aaaaaatctt gcaaaaggac agcgatgtag gtgggcacgt tggtccgaat
     7861 ctaggtgtag tagaattaac gattgcgttt cattatgtat ttgattcacc aaaggataaa
     7921 atcgtttggg atgtttccca tcagtcttat ggtcataagc ttttaactgg tcgaaaaaat
     7981 ggttttgaaa aaggacatta ccgtgaagtg agcggatatt ccaaccaaca tgaaagtaaa
     8041 catgactatt ttacagtggg gcacacatct acatctatca gcttagcaac aggtttagct
     8101 aaagcgagag atctgaagaa agaatcagga aatgtggtag catttatcgg tgacggctcc
     8161 ctaagtggtg gcttagcttt tgaaggattg aacaatggtg gtgttttaca atccaatcta
     8221 atcgtattag taaacgataa tgagatgtct attgatgaga atcatggcag tttatataaa
     8281 aacttagctg agcttcgtca aaccaacggt aaaagcagca ataacctatt taaagcaatg
     8341 ggatttgatt atcgttacgt ggataatgga aacgatctgg aaacggtgat ccgtttattt
     8401 gaagaagtaa aagatattga tcatccgatt gtacttcacg tccatacgga aaaaggtcgc
     8461 ggttatcagc cggcaatcga taataaaatg aaatatcatt ggcatgtacc atttgatctg
     8521 tccaccgggg aggcaaagca agtaacttct ggagaaacat ttaatatgat catcatggat
     8581 tatttggata agaaaatgca aacaagtaca gctcctattg ccgcagtcaa tgcagcgata
     8641 ccaggcgcct ttggactgaa aacattccaa gaaaaacatc cagatcatta ttttgacgtg
     8701 gggatcgctg aacagcactc tatcactttc gcttctgctt tggcttctca gggagtacgt
     8761 ccagtcgtct tccaatcaag tactttcttg caacgggcat atgatcaatt atcacatgat
     8821 ttagcgatca acgagaatcc agtgattatt ttagtcggtg gcggaacgat ctcagctagt
     8881 gaccgaacac accaaggaac atttgacatt ccttacttat caagtatccc gaacatcaaa
     8941 tatcttgctc cagcaacaaa agaggatatg atctctatgc tagattgggc gttcgaacaa
     9001 acagcgcagc ctgtagcaat ccgaattcct acacatggtg tagaacatgg tacagctatc
     9061 gcaaatgatt atagtattcc agcctatcaa acagtaaata atggaggaaa agtagcaatt
     9121 ttagctttag gcggttttct tgaattagga aaagaaacag taaaagcttt tagaaaaaaa
     9181 ggaatcgaag caacgttgat caatccagga acgatcacag aaaatgacgt agaaacatta
     9241 gataaattaa aagaaacaca cgaattagtc atcacattag aagatggtag tttgtcagga
     9301 ggatttggcg aaaatatcag tcgttattat ggtgattctt caatgaacgt attaaatttt
     9361 ggtgcagaga aagaattcac ggatagcgtt cctgtagggc agctttatga acgttatcat
     9421 ttaacaccaa aacaaatcgt tgaagatagt ctgcgtgtct tgaatattca ataattcatt
     9481 atgctcacca tccagataat tgtttatact agtatctgga tggttttttc attataaaaa
     9541 gcaaaggggt ggatgttatg tatcagcaag tagaagaata tgtcaaaaag ttccaagaaa
     9601 aatatcctag ttatcaatca gaaaaattag gtttcttttc atttggggat acagaaaaaa
     9661 tggcagacga gttgagcgaa ctagttgtaa aaaagataaa gactggtaca tcaagtggtt
     9721 acgatctgta taaacaagag aatgagcctt taccaaaagt tggtcaatta gacgtggtcc
     9781 tgaatggaaa aaatgaacca gtttgtatca tcaaaaatat aaatgtgacc atcttgcctt
     9841 ttagtgaagt cacgaaagag catgcttgga aagaaggaga aggggatcgg acattagctt
     9901 attggcggaa agtccatgct gagtttttta aacatgctta taaagaagcg ttgaacatag
     9961 aatttacaga agagaagctg gtcgtatacg aagaatttga agtaattttc ttttagtaag
    10021 aaaggtctgc tgaaagaaat agctcattat tgactcctga atagaaaagg gttacatttt
    10081 tatatgaagc tgtattttta tataacatta ctggaggaga tattgtggat acactggaac
    10141 aaatcaaagg cggagtcgtc gtttcttgtc aagctttgga aaacgagccg ctacacagtt
    10201 cttttattat ggggagaatg gctttggctg caaaagaagg cggagcagtt ggtattcgat
    10261 caaatacgac agaagatatc aatgaaatca agcgaatcgt tgatttaccg gtaataggga
    10321 ttatcaaaag agattacgat gggtcagaag tgtttattac tgcgacagaa aaagaagtgg
    10381 aagaactttt ggagacgcaa gcagaaatca ttgcactgga tgcaacttct cgtaaacgtc
    10441 cgacagaaaa aacaactgct gaacttgtcc aaatgattca cgaacacggt cggttagcta
    10501 tggcagatat ttcgactttt gaggaagcgc ttcaagcgca agaagatggt tttgatcttt
    10561 tgtctacgac tttagcaggc tatacagaat attcacgaaa gacgcagaca cctgattttg
    10621 atttattgaa tcaaatcata gaacaagtcg atatcccagt aattatggaa ggacatactg
    10681 actctcctga acaagtagag aaagcatatg aattaggtgc tttttcagtc gtagttggat
    10741 ctattatcac ccgtccgcaa ttgatcacta aaagatatgt cgaagccgca agtaaagcaa
    10801 ataactaaaa aacacgcacg gcacagattc ttgacaaaaa agaaaaattg atagatagtt
    10861 taaagcagat tctaagaaga tgaaaaaagc attacttttc attttgtctt caagatctgc
    10921 tttttaacga attttataga aagaaggcga aaccatgttt gaacggagtc cttcagaatt
    10981 gacagaacgt gaaaattaca agttgctgat tggttcagtc atccctagac cagtagcagt
    11041 agtaacgact aaatcagcaa aagatatagt gaatatcgca cccttcagtt acttcaatat
    11101 cgtcagttcg aatcgaccca tcttatcctt ggcgatccag agaaaacaag atgcagtaaa
    11161 agacacagca aagaatattc ttgagacaag agaagctgtt atccatatcc ttgatgagga
    11221 caacgtagaa gaagtaaata aaacagcagc caatctgcct aatgaggaaa gcgaactgta
    11281 cctgactgac ttgactttaa ctgattcagt aatcatttca gtaccagcat tgacagaagc
    11341 aaaagttcgt tttgaaacat ccttatatga acatgtcgag atacggaata aaaataaagt
    11401 aacagctgat ttatttttgc tagaagtaca gaaatatcat ttaaatgaaa cagtctatga
    11461 tgataagaca ggaaggatcg atccaagaaa attgaatgct gtaagccgtt tggcaggaaa
    11521 tgattatgca gcaataggag aaattttcac gattgcacgt cccgaataaa aaggagcact
    11581 agttatgtta aaaataagct ataaaccatc gacagattca aaagaaatga aaaaagaata
    11641 tgaaacagtg aatgattttc tgcaagggca gtacttagaa gttccgccat tgcaagacca
    11701 ttttgtcgtt actactgtta ctttagatgg taaggaaata gaaatgccag atcaaacgat
    11761 tagcggattg tttaattact ttaacaaata aatatttatt ccgaaaagtg aaaaaaacat
    11821 ataaaaaaac acataaaact aagaaattat caaagaatcc tggtgcaact gaaagaattt
    11881 gcaacgggat tctttcaact tttttgttat ttttttctaa aaaatattaa agattgttaa
    11941 atttcactat ttcatttcaa attccattgc ctaatcatta ggaaaagtct atcatgaact
    12001 cgtaaataga atatagtaat aaccttgtta ggtgaggctc ctatacaaac ataggctgtt
    12061 gcccagaaac gtcgaaagtc gccaatgggt aaaacagaaa ctgtcggatt aaggcttttt
    12121 ccaatagcta agaatgtttt ctttacgttg tatagtgcta aaactcggac gatgagatga
    12181 tgctaggtca gaattcttgt tcgaaaaggt ttctgacctt ttctttatat ttttgtgact
    12241 gacttatttg atgagacaaa ctcgctcctc acctgacaag tggattgctg tgtgaggagg
    12301 agatgcggtg aaaaaaagaa aaaagatcga ttggtggatt ttgggtcctt atttgacttt
    12361 gtccatgatc ggtcttttag aagtatacag tgccagttct tatcgtttat tgcaagccga
    12421 tgaaaataca aaaagtctat tattgcgtca actaattttc atatttttga gttggagcgt
    12481 gatcttctta gctcgttcag tcaaactaca ctatttactt caccctaaaa tagcaggata
    12541 cggtttagcc ttatcgattt tctttttagt attagtaaga atagggatat tcggtgtcac
    12601 tgtcaacggc gcacaacgtt ggatctctct gtttggcatt caattccagc cttctgaact
    12661 ggcaaatctt tttttgattt tttatttaag ctggtttttt cgtgacggaa atagtagccc
    12721 aaaagatcta aaaaaaccat tcctgattac agtaggtata acttttctga ttttatttca
    12781 gccaaagatt gctggagcat tgatgatcct ttcgattgcg tgggtcatat tttgggcagc
    12841 ggcggttcca tttaaaaaag ggatctatct aatcgttact ttttctgcat tgctgattgg
    12901 agcagcaggc ggggtattat atttaggaaa taaaggttgg cttccacaga tgtttaatca
    12961 tgcttatgaa aggatcgcga ctttaagaga ttcttttatc gatagtcatg gtgcgggcta
    13021 tcaaatgacc cattcgtttt atgcactata taatggtggc atatttgggc ggggattagg
    13081 aaatagtatc acaaaaaaag ggtatcttcc agagacagaa acagacttta ttttttcgat
    13141 tattacagaa gagcttgggc taatcggtgc attgtgtgtg cttttcttat tgttctcatt
    13201 atgcatgaga atcttttgtt taagcagtcg ctgtaaaaat cagcaagctg gattgtttct
    13261 tttaggattc ggaactttgc tattcgttca gacgataatg aacgtgggaa gtatcgcggg
    13321 gctgatgcca atgactggtg tgccattgcc atttgttagc tacggaggaa caagttatct
    13381 gatcctttct ttaggaatcg gaatcacgtt gaatatttct tcaaagatac aggccgaaga
    13441 gttgccgctc tatcgacctg aaaaacaata aagagaaact gagaaactct cagtttgaat
    13501 tatcataaag tacaatcttt tatttttcaa gcgagaggtt cttttagaaa gaggaattcg
    13561 caatgaaagt atctcaaaaa atcatattat ctattttgtt actgcttaca ttagtagttg
    13621 gatatttttc gattgagttt atccatggat tttcgtctgc aaaacagagt tcaactgtga
    13681 aaaaggtaga gccgaaaagt gtccctacca cactaaatgt ggctttgatt ggttcagatg
    13741 ctcgttcgaa agacgaaaac ggtcgctcgg attcacttct ggttgcacaa tacaaccaga
    13801 aaacacaaca agcaaaactg atctctatca tgagagattc atatgtcgat attccaggtt
    13861 acggaatgaa taagataaat gcagcttact cttatggggg agttgattta ttaaatcaaa
    13921 cattaaagga aaatttcaaa tttgaagctc cctattatgc aagtatcaca tttcaagatt
    13981 ttatcgattg cgttaatgaa ctgtttcctg gtggagtcaa gatcgatgca gaaaaatctc
    14041 tagatttaga tggcgtatat ataaacaaag gagagcaagt gatggatggt aatactttac
    14101 tccagtatgc tcgatttcgg gaagacaaag aaggagactt cgggaggatc agaaggcagc
    14161 agcaagtgat caaagctatc tctcagcagc tgaaagacgt aacttcaata ttcaagctac
    14221 ccaaagcggt tgggaaatta cttggtagta tccaaacgaa ccttccagaa agtgtgcttc
    14281 tggattgtgg tatggatttc ctcaaagaca tatgttagaa taaacaggta taaatagtga
    14341 aataaaggaa tgacaagcaa gagaaggagg aaaaaatgaa aagaagtgac aagcacggca
    14401 aaaatcgaac aggcgcttat attgccggcg cagtgatctt aatagcagct gcgggtggcg
    14461 gttattttta ctaccagcac tatcaagaaa cccaagcagt agaagctgga gaaaagacag
    14521 ttgagcaatt tgtccaagct ttgaacaagg gagattataa caaagctgca gaaatgacat
    14581 caaaaaaggc ggcaaataaa agtgcattat ctgaaaaaga gatcttagat aaataccaga
    14641 atatatacgg tgctgctgat gtcaaaggac ttcagatatc gaatctaaaa gtagataaaa
    14701 aagacgattc tacttacagt ttttcatata aagcaaagat gaatacctca ttaggtgaat
    14761 tgaaagatct ttcttataaa ggaacattag acagaaatga tggtcaaacc acgatcaact
    14821 ggcaacccaa cttggttttt ccagaaatgg aaggaaatga caaagtaagc ctgaccacac
    14881 aagaagcagc aagagggaat attatagatc gaaatggtga accattagca acaacaggca
    14941 aactaaaaca attaggagtc gttccaagta aacttggaga tggaggcgaa aaaacagcca
    15001 atatcaaagc gattgcttcc tccttcgact taacagaaga tgctatcaat caggcgattt
    15061 cacaaagctg ggtacaaccc gattactttg tcccattgaa aatcattgat ggagcaacgc
    15121 cagaacttcc agctggagct accattcaag aagtagacgg cagatattat cctttgggtg
    15181 aagcagctgc tcagctgatt ggttacgtgg gagatatcac agcagaagat attgataaga
    15241 atccagaatt aagcagtaat ggaaaaatcg gtcgctcagg tttggagatg gcttttgata
    15301 aggatcttcg tgggacaaca ggtgggaaat taagcattac agatgcagac ggtgtagaga
    15361 aaaaagttct gatcgaacat gaagttcaaa atggcaaaga tatcaaattg acaatcgatg
    15421 cgaaggcaca aaaaacagct tttgacagtc taggaggaaa agctggatca actgttgcga
    15481 caacgccaaa aaccggtgat cttcttgcgc ttgctagctc tccaagctat gatccaaaca
    15541 aaatgacaaa cgggatctca caagaagatt acaaagctta tgaagaaaat cctgaacagc
    15601 cattcatcag ccgatttgcg acaggttatg ctcctggatc tacgtttaaa atgattacag
    15661 cagcaatcgg tctcgacaac ggcactatcg atccaaatga agtgttgacg atcaacgggc
    15721 ttaaatggca aaaagacagt tcttggggat cttatcaagt aacgcgtgtc agtagtgatg
    15781 tatcacaagt agacttaaaa actgctttga tctattccga taatatatat gcggcacaag
    15841 aaacgttgaa aatgggtgag aaaaaatttc gtacaggctt agataaattc atttttggtg
    15901 aagaccttga tttgccaatt agtatgaatc cagcacaaat ttctaatgaa gatagcttta
    15961 actcagatat cttgctagct gatactggat atggacaggg cgaacttcta attaatccta
    16021 tccagcaagc agcaatgtat tctgtttttg ccaacaatgg cacacttgtc tatcctaaat
    16081 tgattgcaga taaagagaca aaagataaga agaatgtcat cggcgaaaca gcattacaaa
    16141 cgatcgtgcc agatctgaga gaagttgtgc aagatgtaaa tggtacagca cattctcttt
    16201 ctgctttagg gattccgttg gcagcgaaaa ctggtacagc ggaaatcaaa gaaaaacagg
    16261 atgtaaaggg gaaagagaac agtttcttgt ttgctttcaa tcctgataac caaggttata
    16321 tgatggtcag catgttggaa aataaagaag atgatgattc agcaaccaaa cgagcatcgg
    16381 aactattgca atacctcaac caaaattatc aataaaaaat tgctttttaa gtacagtaaa
    16441 aggactgtgg cgttatcgtc acagtccttt tttcaaataa acggttttca cgatgtctct
    16501 ttcacaactt ctttttgatt tactcacact tcataaactt tttttgtaaa atatttgaga
    16561 acaagtggag aaataagtgt acttaataaa atcacgataa ctaaaggtga atagtactgt
    16621 ggttcgatca attgactttg ctgtccaatt tgtaaaatga tcaaagccat ttctccacgc
    16681 gaaatcatac cagcaccgac catcaatgaa ctatgttgag aaaaaccggc aatctgagca
    16741 ccaaaataac ctccaagcaa tttggtcaaa atcgctacaa gcgtcaatat agaaataaag
    16801 agtagttgtt cactaaagtc agaaaaatca acttctaatc ctacactgac aaaaaatact
    16861 ggtataaata ctgcatatcc taaagcctct acattgtggt atacttcttc tttaaccttt
    16921 gtctgactga cagctatccc agcaaaaaaa gcaccaatga cagagctaag cccaacaaga
    16981 tccgctacgt aagacattcc taaacaaata actagcgata cgatgataat agaagaattt
    17041 gcgtacactt tttctgcaaa tgaaagtagg aaaggcgcaa tccatttgac taataagaaa
    17101 ataaaaacaa aataaattag ctgttcaatc aaaacaagcg gtaaagaagc attgctagaa
    17161 gcttcccccg ttagaaaaga taggctgaaa cttagaacta aaacgactaa aatgtcatct
    17221 accacagaag cacccaaaat tgttgatcct tcttttgtat tcactacgtt tagttctttt
    17281 aatacttcta ctgaaatact gacagaagtt gccgcaagaa taatcccaaa gaaaatcgct
    17341 tcatttcccg aaacttggaa tgctttgcct gtcaaccagc caaaaacaac cgggaagatg
    17401 atcccaagca aagcaacgaa cataccaggt cggaaatatt ttttcaaaag agataaatca
    17461 ctttccagac cagctaaaaa cattaataag atcacaccaa tctccgaaaa gtcatggact
    17521 agaatatcag gtttgaccca attcaatcca gcttggccta gcaaaacacc aacaagtaat
    17581 tgtccaatca ctgctgggat acctaatctc cgcgagatat gtgagccaat cgttgttgaa
    17641 atcaatatca ggcataaaat accaataaat tccattctca tcattccttt gtataaaata
    17701 tagcccgaac ttagcataac atacattatt gaaaaaatag gaaaatatac tcacaaaaaa
    17761 accaacaatc caaaagattt ttttggattg ttggttttta tacggtctat tttaataact
    17821 aaaaatgatt aacgtatagt catttttctt attctgatga aattgataat ctcctggatg
    17881 cccatgaaaa tcaaaataaa gccgaatatt tggaatagaa gcatcaaact tttgaatgga
    17941 ttgaacaata agacgaatcc gccaatcaac aacagaatac tactataaac actccaactt
    18001 ttagcttttg tattcatgct gaagaacaac tggaacaagc tgttgatgat aacagctaat
    18061 ccaagaagaa tcggcaggat agaaacgatt gcaggtgcaa aagcatagac gatcagagct
    18121 aagatcagga aaaaaagacc actgaacaga ccgatactcc aagaacctgt tttcctttta
    18181 aaacggtaag cctcaaaaag attcaacaac ccaagcaaaa caagataggc ggtgatcaga
    18241 taaccgataa aatgaaaaac agcagaaggg tttagcacaa tcgcaatccc agaaataata
    18301 taaacagcag ctcttaataa ggcgtatttt tgaatatttt gtttaatcgt ctcaaacata
    18361 taagaaaacc tcctttgact tattttaacg tgaactagtc attttaccaa attaaatgtt
    18421 acaaaatgag cgaaggagaa gaatatctct ggaaaaatca acttctgagg atcttttcca
    18481 tttttttgcc ttttgctagc tcatcgatca ttttatctaa ataacgaatt tctttcatca
    18541 agttatcctc gatgtcttct actcgtacac cacaaacaac acctgtaatc aaggaacgtg
    18601 aaggattggg gacaggggct tccgcaaaaa attgttcgaa agtcacttgt ttttgtagtt
    18661 gatattctag ttctgtttgg gaataacccg tcagccaaca aatgatttca tctacttcct
    18721 cttttatacg atcttttctc tccgcttttg ctacatacat aggataaacg cttgcaaaac
    18781 ttgtcgtaaa aatacgattt tgcgccataa tcagtcacct ttcttttttt caaattattt
    18841 actacttata gtatataaga aaaagagaag aaaaagcgat tattcaaaaa aacaaaaagg
    18901 attgtgacaa aattcttgtc accatccttt tttccgctct ctgacttttt acaatcttca
    18961 tagctcaagt gcgcatcaga taatcgaatg ctcctaaagc tgcagtagct cctgaaccca
    19021 ttgaaacaat gatttgtttg tatggactat ctgtacaatc accagctgcg aaaacgccgg
    19081 ggatattcgt tgcgccattt ttctcggtca ctatttcatt tcttggattc atttcgatcg
    19141 ttctatctag ccaatccgta ttagggagta agccgactag aatgaatact cctgaaacag
    19201 ctagtgtatg catctccatc gtacggcgat cgatatagga gagagattct acctgattcg
    19261 taccgctgat ttctttcgtc gctgcatgtt tgataacagt gacattcgca agagagacta
    19321 gtttatcctg taagatttga tctgctttta attctggtaa gaattctaga acatatacat
    19381 gttttgctaa cccagccaaa tcgattgctg cttcaacacc agaattacca ccaccgataa
    19441 cagcaatctc ttttccttta aataaagggc cgtcacagtg tgggcaataa gcaattcctt
    19501 tatttttaaa ctctttttcg cctggtacat tgatagaacg ccagtgcgca cctgtcgcga
    19561 tgatggcggt ttttgcttta agtgatatac cgttagctaa gccaatatta ataaaatgtt
    19621 gctctttatt taaggaaacg gcctgttgat tcatcataat gtcgataggg taactacgca
    19681 catgactttc tacttgctgc atcaattttg gaccttctgt gtaagaggta ccaatcatat
    19741 tttcaatccc tagtgtttca acgacttgtc cgccgaaaga atcagcgaca agtcctgtac
    19801 ggattccttt acgtgctgca tagatagctg cactactagc tgctggtcca ccgccaatca
    19861 ctaatacatc ataaggttct ttttcaaaca actcttcttc aatcaaaggg ccagtgattt
    19921 tttcaacaat ttgttcgatc gtcatgcgtc cgctactaaa ttcttctcca tttaaaaaca
    19981 cagttggcac tgccatgatt tttttatcat cgatttcttt ttggaacatc ccaccttcaa
    20041 tcatcgtatg agtaatattt ggatttaata cagctaatat attcaatgcc tgtacaacgt
    20101 cggggcaatt atgacaagtc aagctaacat aactgtcaaa atgcaatgtt tgtgttattt
    20161 ttttgatttt aagactcaaa gattcactga ttttcggtgc ccgtccacta atttgtaaca
    20221 tagccagtaa aaaagaggaa aattcatgac cgagggggat accagcaaaa acgattccac
    20281 tttctttgcc aactggattg atagaaaaac taggtgtgcg catcgaatct cttttttccc
    20341 aagagatttt aggagacatt tctgctattt ccttcgtaaa ttcagctagt ttttgactat
    20401 tttcactatt atccaaacta acagaaaaaa caataggaga ttcaatcagt gctaaatatt
    20461 gttcgagatt ttgtttagtt tctttgtcta acatatccac cacctaaatc tttccaacaa
    20521 gatcaaaact tggtttcaat gtttcgccac tttctttcca ttttgccgga caaacttcac
    20581 ctgggtgggt acgtacatat tgagcagcgc gaatcttatc aatcagtgca ctagcgtcac
    20641 gaccgattcc atctgcattg atttccatag cttgaacgat tccgtctgga tcaataataa
    20701 atgttcctcg ctgagctaat ccttgctctt catccaaaac atcaaaaata cgagaaatct
    20761 gatgagacgg atcaccgatc atcacgtatt cgattttacc gatagcatct gatgtatcat
    20821 gccatgcttt gtgagtaaag tgggtatcgg tagagcaaga aaagacttca acacctaaag
    20881 atttcaatgt actgtattgt tcttgtaagt cttctaattc agtaggacaa acaaaagtaa
    20941 aatcagctgg atagaagcaa agaatactcc agttccccat aagatctttt tcactgactt
    21001 ctaaaaattc tccttgatga taagcatgtg cattaaattc ttcgattttt ttcccaatca
    21061 atgacatagt aaaacctcca ataataatta tttatttata attattgtaa tttagttgga
    21121 gttgcaaagt caatggatat tttgttattt aataaagatt actcaaaaac gaaggttaat
    21181 tataaaaata gaagaggtcg caagaaaatg ctactgctgg aaatactcgg gataacgttg
    21241 agtaacagtt tcctgttctt tcagcatcat gtgtaagtgc aatcccgttt gaaatcgagc
    21301 ttcttctggt tttttatcaa ctaaagcctt gagtaaatca taatgttgtt ccatgatcgt
    21361 ttcccatgga agcccctgaa tggtcactcg taaccagcgg aaacgttcta aatgggtatt
    21421 atggtcatct aaccagttcc aaacttcttt tcttcctgca atttcgaaac aagcttcatg
    21481 aaataaatta tctgcacgga aaaaagcgcg ttcgtcattt attttgactg ctttttcttg
    21541 ttcttctaaa atcgtttcta gcactattcg tgtttgtgtg tccaatttgg aacaacattc
    21601 aatcatgatt tgctcttcca actgttctcg gacaaagcga gcattttctg cagaaacaag
    21661 attaattttt gaaacatacg taccactttg aggtatcgta tacacgagtt cttgctgacg
    21721 tagttgaatc aatgcttcgc gaatgggagt ccggccgata gcaagatgtt cctccagtgc
    21781 cttttcagat attttccttc caggttctaa gtcagcatag ataatttgtt tacgaatcgc
    21841 ttgataagct tgagattgca agctgctttt catctttatt cctccaaaat catttctatg
    21901 aacgtagtat aacagaagag gtctgaaaaa caatctggta tgtcacatta actagtaaat
    21961 ttgtataaat agtaaaattt tggttgacat agcgattttt attgcataaa attcacttga
    22021 atacaaaaac taatatgtta gtttaggagg ggaaaaatga ttgatttaaa caactggagg
    22081 aactttatcc ctgaattatc tcaaaaagaa gtagaaatac ctcattttga ccgtgcatca
    22141 ttaagagaaa atggacaaaa aaatcctgtc tggatccatt tcggtggcgg aaatctttat
    22201 cgaggatttc atgcagaaat cgctcaacgt ttagcggatc taggcgaatt gtccactggt
    22261 gtcattgttt gtgaaacatt cgatgaagaa ataattgaaa gggcttacca agattttgat
    22321 gatttgattc ttgaagtggt catgccaagc agtggagagt tcgtcaaacg tatattagct
    22381 gcgacagctg acagtctgta ttgtcatcca gattgttctg aacactttga taagatgatt
    22441 cgacactttg aaaatcctag tttacaatta gttacttttg ctattactga aaaaggttat
    22501 cgattgaaag atacaaataa taaatatata ggaccgattg cagtcgatat aaaaaatggt
    22561 ccaaagaagc catcacatac aatgagtatt cttgctgcgc tactttataa acgtttttta
    22621 tctggagctt ggccgattgc acttgtctct acagataatt tttcacaaaa cggtagtcaa
    22681 ttacgtactt ctttgctaac gatcgcaaaa gaatggcaaa aggaaggtta tgtacaaaat
    22741 acatttatcc aatacttatc gaatgaagaa attgttgcgt ttccatggac gatgattgat
    22801 cgtatcaccc cgaatccttc cttgtcagtt agcaaaatgt tggcaaaaga gggcttttca
    22861 aatatgtcga ttttccaaac cgcaaaagga aataatattg ctccatttgt caatacagag
    22921 gacatccatt atttaatcat ggaagatcat tttcccaatg gacgtccgaa attcgaaaaa
    22981 gctggagtat tcttgactga tcgtgcaacg gtagataaag cagatacgat gaaggtgacg
    23041 acttgcctta atcctttgca tacgtcttta gctattatgg gttgtttact ggggtatagg
    23101 agcatagctg aggagatgaa agatccagat ttatcgctgc tagtttctga aatcgggtac
    23161 aatgaaggcc tcccagttgt ggagtctcca ggtatcattg atccaaagat ctttctggaa
    23221 gaagtaatta ctgtgcgctt ttctaatcca aatattcctg atatgcctca gcgaatcgcc
    23281 acggatacct ctcaaaaggt ggcgattcgt tatggtgaga cgattaaaaa atatatggaa
    23341 atagaagtta aagacccgca gacacttgta ttcataccat tgactctcgc tgcttggctt
    23401 cgatacctta ttggcattga tgatcaagga aaaccattca tcccgagtcc agacccacta
    23461 ttgacagact ttcagcataa gctttccgat attcaactgg gtacgcaaga tacatcacat
    23521 attcatgaga aaatggctcc tattttgtca aataaagaaa tttttggtgt agatctttac
    23581 caagcagagc ttggcagaaa aatagaagaa atgttcatca agatggtagc aggaccgaat
    23641 gccgtacgaa aaacattaca ggattactta caagaacatg gaggaaattt atcatggaaa
    23701 tgacttttag atggtacgga tcacaggaag aaaagattac cttagatgaa atcagacaga
    23761 ttcctgcggt caaagggatt gtaggcacat tgatggatat accagtagga gaggtatggc
    23821 ctagggagag gatcaaagaa ctaaaagaag aaattgagtc tcgtgggtta acactaaaag
    23881 taattgaaag cgtcaatgtg catgatgcca ttaaaattgg attaccttcc cgtgagcagt
    23941 atatcgaaaa ctataagcag acaatccgaa acttggcaga atatggaata gaagtcatct
    24001 gctataattt tatgcctgta tttgactggg tgaaatctga ccttgactac cgcttagaag
    24061 acggctcatc cacgttagct tttatatctg atgatattcc agatgatcca agagaaatcg
    24121 tcgcgcgcat cgaacattcc agcaatgaat ttgaattgcc gggatgggaa cctgagcgct
    24181 tagctcatat caaatccttg cttgaagcct atgcatcagt agacgaagaa aaacttcgag
    24241 aaaattttgc ctattttctc caatcaatca tcccaacatg tgaagaggta ggggtcaaaa
    24301 tggctgtaca tccagatgat ccgccatact cgatttttgg attacctcga atcgtaaaga
    24361 atcgagaaga tttagattgg ttgtgccgca tagtagacag cccttataac ggaatcactc
    24421 tttgtacagg gagtattgca gaagatccag aaaatgatgt atatgggatt ttagcagaat
    24481 tttgtcaaag agatcgaatc ccatttgcac atgttcgaaa tattaaattt ttagctaatc
    24541 gagatttcta tgaggcaccg cataaatccg aatacggttc attagacatg taccgcatac
    24601 taaaaacaat gtatgacaac ggatttaacg gatacatccg tcctgatcat ggaagaatga
    24661 tctggaatga gacaggaaga ccaggatacg gcttatatga tcgagcatta ggtgctgttt
    24721 atctaaatgg attatgggaa gcaatcgaaa aaggaaacaa aagtaaagat aattagtgaa
    24781 aaaaactgta attttattat gtaaataaga aggatgttca aattcttgat acataaatcc
    24841 ggaggttgtt aataagcatt cggatttatg tatcttattt ggtctaggaa gtaacttgat
    24901 tttctttttt cgattgatcc atgagtgctt cttcttttaa ttcaaagata tattctgaga
    24961 tcctcataat cacttttttc cagtcttctg gtgtgatgat tccttttaaa atatagattt
    25021 ttttgatatg agaaggcaat tgtttcaaag ggcagtcgct gataacgatg ttgcagttct
    25081 ccataaaatc attttgtatt ttgatcgatt catttgaaaa aatttgttct atcttaacga
    25141 tcagatcttt actgatataa aaatcacgcg tatagttcac accaatcaca attcgcggct
    25201 gttttttgta gcgatcatac aaatgtacta ggtcttcaac catccattgc acattttcct
    25261 tttctaaaat aactggtaac aagtgttgat tttgttctct gaaatccata taaaattgtt
    25321 caatggcggt ttggacttct ttcaacacgc tatgttcttg ataaagcatg aaagtaggaa
    25381 ggggcgtatt tggttgattg aagttaagaa atccattata aagtttattt cgtgcgaatg
    25441 ccaatgagta aagaatcttt tctttttcag gtataaacaa atcaaataca gtacaaaagg
    25501 actccaccat ttctcttgaa taaagaacat gaggaacgtt tgcttttttt aaaagtttgt
    25561 attgctccaa gcttgtttct tccgattcga ttttcgtgat agaaagtcga gctaaaatat
    25621 tcactaagat gcgttgttcg cgacaaattg gcagattagg tcgcaaaata tctacagagg
    25681 gatcaataaa aaagtcaata tattcatact ctgtgaattc tttttgtacg tctttcacag
    25741 aagttgcagg aaaattctct ttacatattt ttaatagaaa atataattta tctatcatac
    25801 cgctatttag tcggtcaagt acttcttttt taaaaaaagt attcatcata aaagccacta
    25861 cttgcggatc ttcatcataa aattctgtag cagtacttga aaagatgttc caataaatat
    25921 ctaatgctgc atattgaatt tgtacctctt ttccagaaaa agactttctg ccattattag
    25981 aaaaggagat agacaaaccg tacaatgcta aaaatgcatt aatctttttc attttcgtat
    26041 acaaataaga ggcactatag tttgtttgct gacttaaact gataatcgaa tttgtctgac
    26101 agctgaacaa agaagtcaag atctgataac tgactgattc gttgcagtat cttccaaata
    26161 gacgataata gcagtaagcc ggatcaattc cttcaaaatt caactttaaa aaaggttttt
    26221 gcttatcaat atgcagttct gttagattga gacgctcaaa gtcttgttct aattctttga
    26281 tgtatagtaa aaaatttgat tcagacatat ggaaatgatc tgctaaatat tgtgtagtaa
    26341 taaagaaacg atgttcagat aggaaaagaa aaatatctaa ttttgtattg ctttttttct
    26401 tcaagagaaa cgttttaatc gcttcttgag tttgcatgaa agaggctcct ttttacttaa
    26461 attgttaaaa cttgaaaata ttactgtttt tttcataaat ttagacaaaa aaacagtaaa
    26521 catataggta gaatctctac tatcttattt cttgcataca aaaaaatcaa ggagtgagag
    26581 agttatgaga tattcaacca tcttaatatc aacatttgca gccttaagct tatcgcttac
    26641 tataaatcag acagtccaca cagaggagat agaaacagcc aaatggacag aaaccaagtg
    26701 ggccaacgtc aatcgaatta cctttcagga tgaattttca tccttcaatt caggagtcgc
    26761 aaaattaaga aaaggacaaa cagcgaccat caaagcaaaa attgactatt caggagactt
    26821 agcacctata ctacaatcag ctgttacatt tgagaatgtt gatcccgctt tgtcgattgg
    26881 ctatgacgag aacactttga ctatccaaca caagcaagcg atatttacct tcaccgttac
    26941 attaaatcag gacttgttaa aaccagcgct ttttactgtc aaggtatcag atggactacc
    27001 gaacgatcat catcatttaa cgccgtatag tcagcggcag acaattgagc aagattcagc
    27061 tatcgactca ggaggagatc ttgttgaaga accaacagat aaaccagaaa atgaaaataa
    27121 gccagaagta ccgcctacag aaaatccaga cggagaacag aaaccggaaa tagaacctgg
    27181 agaagaaccc gatacagaaa ctcaaccaga gccagacaat gaatcaaaac ctgaaataac
    27241 ccctggagaa aaaccagatg tagacccaga agaaaaaccg gatgtgactc cagaaccaga
    27301 tacggattct ggaaaccaaa cagtaccgga aacaaatcca gacacagata atgaaactga
    27361 aaatccagaa aaaccagagg tagccccaga agaaaaacca gatgtaactc cagaaccaga
    27421 tacggattct ggaaaccaaa cagtaccgga aacaaatcca gacacagata atgaaactga
    27481 aaatccagaa aaactagagg tagatccaga agaaaaacca gatgtgactc cagaaccaga
    27541 tacggacgca agagaccaag gaataccaga aaaaataaac aaaaaaacaa ttcaagagga
    27601 tggaaaaaaa gagagtaaaa aatcaaatct agcaattcta aaaataaatg aagagcagct
    27661 gaataaaaaa tcacgaatat ttgatagcgc ccaatcagct gaaaccttaa aaagtagcaa
    27721 ggatacaacc tttgctagtc ctgaaacgaa aaataaacag ctaccaaaat ccggagagtc
    27781 acaaaacaaa gttatactgt ggagcggaat cattctatta agtatagcaa caatgctttc
    27841 tgcaaaaaga tttaaacaaa acagatctct ctaacatttg aataatgtct gtgaacgcat
    27901 ttttcaaata aaaagaaata tccagaaaac gaccccaaaa taccagattg aaatctgata
    27961 tttggggtcg ttcatacttt atctttcttc atatttattt taagggatat ctgtttcttt
    28021 tggaagatgg ttcgggatct ttttcacttt caatacatct aaaaagaagg taaagatcgg
    28081 aatgccgaca atcaagcccc aaacgccaaa gaaccgttcg ctaacaagta agaccacaaa
    28141 tgtataaaaa atcggcagtt ctgttttgct ggacatcaac ttcggattca gcacataaga
    28201 ctcaattaga tggacaatca aaatcgtaat caggatataa acaacatctc ggatgcctcc
    28261 ttgggaataa gctatgaaag atagaggaat acaagaaatg ataacacctg ctactgggat
    28321 cagacttaaa atgaaaatca tgatcgctaa actcagcagc tgatgaaagc caaatacagc
    28381 caaggcgatt gtagtgagca acgtgtttaa aagagcgata acaaattgag cttctaatac
    28441 caaaccaaat gtattgacaa acttatcggc aaaatagtaa atatcttgaa agaaccaaga
    28501 aaattcactt tttaaaaaca atcttgagaa tacaattgtt tttttctttt caatcataaa
    28561 aaagaaactt aagatgaatg acatagcaaa cgccactgcc atttttccta tatcctgtaa
    28621 atagccaagc gcaatcgaag cgccattttg aagctgttca aaaaaatcgt ttcgctcaat
    28681 ataagtatgg atatattgta aaacaggatc tgcatctttt ggctgatgct cgtaaaaatc
    28741 aataacggac tggatcatat caaatgtttg atgaataagc atcgggatat actttgtgaa
    28801 tgccagatag ataaggtaaa cgatcagtgt ataaatcatg atagctaaaa cacctgtagg
    28861 gattcggaca aatcgctgaa tgatttgaac aagatgaatc atcaaataag taaagataaa
    28921 tgtgagcaaa atggttgtaa tcatacttct aactaagaaa agaacaaaaa cgacaaataa
    28981 taaaacagtg agacggcgta ttttttcatt cgccaaaaaa cgctgataaa cggtcaaatc
    29041 gacaacctcc aaataacaaa aaataaacac gttattcatt atatgatgga atatgaaaat
    29101 aagaaagaga aagagcaaaa agaaaaaccg ctaaatgacg ttatcttatg agttggtttt
    29161 cctcataaaa tcgttcatct agcagtttca gccctttttc tggtcttgat gaaaattatc
    29221 tagcattttt cgggtcagat gttgcagtgt atccaattct tcctgttcaa gtggaagata
    29281 agacttgatc ttttgtggaa taccttctgc ttgtttttgc aacgcagcac cttgaggagt
    29341 cagcgtaaca tttacgattc tttcatcacc cgtttcacgc tgacgattaa tataattttt
    29401 ttctgctaac ttttttagta agggagtcag cgtaccagaa tccagacaca aatcatttcc
    29461 tagctctttc actgaagtct ttttcttttc ccataaaact aacatcacaa gatactgggt
    29521 ataggtaaga ttcagcggct tgaggaggga agtgtagtgt ctaatgattt ccttagaggc
    29581 ggcatataaa ggaaaacata cttgattaac taaatataga tcttccattt ttccacctcc
    29641 gctgttttac tatagtgtat catagcggat ttacacaaaa aagtctattc actaactgct
    29701 ttcagagtaa cttctatatt tccagctgta gcttttgaat acggacacac tttatgcgct
    29761 gcctctaata attcttgcgt ttcttccaga gaaagacctt cgatagatcc ttcaatctct
    29821 acacctaaga caaagctcgt ttcactttct gaataaaggg aaacttgtgc actgacagta
    29881 gaagccccag agatattttt ttgctgtttg atcaattcta atgcactgtt aaaacatgca
    29941 ctgaaaccag ctgcaaacaa ttgttcaggg ttggttgcat tttcgacgcg tttaccagga
    30001 gctgcaatat tcaattcgaa tgtacgatcg ggattatgaa cttctccaga gcgtccgcct
    30061 gtgttgatca ttttcgtaga atacattttt ttcataaaat taccaccttt aattatattt
    30121 tatacaattg aattttacac aatataattg tggaaagcaa agaaatggat tggcataaga
    30181 taaataagag aaagcacatg aaacagacgc gctttctcga atagcaaaca tatacaaaaa
    30241 aatatcagag acaaaaacta atctcatcat aaacattaca tatatttctc aaaaagttgt
    30301 tttctttctt caaaacttac ttcacgatgg acctgatgaa gcatccataa ataaccaaaa
    30361 ggatctctga acattgagtt agtcacacca aagtccatca tttctgtcat ttcttgaatc
    30421 tctacacagc catttcgtaa tgctttatta tgtgtgtcct cgatatcaga gacgatgata
    30481 ttgaaccaga tcgtatttgg atgttcagca gtaggagcag tcaaaccaag agacgtgttt
    30541 tcatccagca tatggaatct cgtgccatac aaggtaaaaa tgacttcatt attgcctcta
    30601 gggtaatctg ttgcctcaat cagttgtatt tcatcaaaga ttgtttgata cagttctagt
    30661 gctttcaaac tatcatttaa tacaaaatct atttccgttc ctttatgcat aaagatctcc
    30721 tctccttttc agtcgaacag ttactagatt cttttattat accagttaaa gaaaacgcat
    30781 tcaaaaaaat agcggattta cttaccaact cattcgattg gatgataagt aaatccgcgt
    30841 cccattactt ctgctacgct gctgacaatg acaaaggcac ttggatcgat ttcttctatc
    30901 aatggtttca ttcgggaaaa ttcatgatca gtcacaacga tcaataaaac ctcttttgac
    30961 tttcgattat acccgccttt tgcctctagc agggtaaggc cacgtccaat ctcagcactt
    31021 aagcgaaggc ggatgtcttc caaataatgt tcactcatga ccataacaga tttctttcga
    31081 ttgatacctg tttcgatata agtcatgaca attgaagtga ttgcaataga taaaatagca
    31141 taaagaaact cttctattcc aaaaacaaac agattaagag aaacaacgaa tgcgtctgta
    31201 aaaaataagc caatcgaagt attcaagtgg aaatattttt taaaaatgag gggagggatc
    31261 gtcgtaccgc cgctagaaga gttgttttta tataaaatcg caactcctaa agcaaaaatc
    31321 gcacttccaa aaatgacaga caacaaccga tctgaagtaa tcattgtttc tggaactatt
    31381 gcaatgatta aaggaaaaaa caagcttccg aaaagtactt taaaaaaggg ttttttacct
    31441 aaaaatagaa gagctagtac caacataacg atattcaaca ctaaaataac tatagctcgg
    31501 tcaaatccta aaacagcttc cagtaatatt cctagaccgc tgacacctcc agcagcaata
    31561 tgatgaggcc ctaaaaacat attgatagcg actcctaata tgaagagggc aaaaataatc
    31621 ttaatgtatg tccatataaa ttgtctcgtt tctttcattt atccacttcc ttaattcatt
    31681 aataatactc ttatcttatt ataacttact tttcacctta agttaacttt aattaataaa
    31741 aaaattaggc tagtctaaat tattatgttt cattcttcta gaatatactt ctacgttaaa
    31801 aactgttaaa aaacttttac tttagaaaat tggagaaatt gatcgcttaa gataaattca
    31861 ggacactaaa tagtacagga agtagattaa aacttttaaa agttttaagt ctatctattt
    31921 ggcaagttta aaaaataaaa attcggtatc aaaaaactgt taccgataac atttttgaaa
    31981 gcgttgtctt ttgtttctct atcacttata gtgatgacgt catattaaaa cacaaggagg
    32041 aaatcaatgg atacagctgc aatttatcat cgaccggaaa gtgaatatgc atttctatat
    32101 acgaatacaa gatttcgtat tcggcttcgt actaggaaaa atgatatcaa aaaagtatat
    32161 gtgctttgtg gagatccata taccataaca actgaaaaat ggtatcaaaa acaacgacca
    32221 atgaaaaaat gtctaagtac caatgttcat gattattggg aaattgaagt gacgagcgaa
    32281 acaaggcgct tacagtatgc gttccatgtt gttggggaag ataacacgga ctgtttttac
    32341 ggtgatcaag gaatttttcc ttatcgagaa aatgtgatta ctgaaccaaa tttttatttt
    32401 cgaatacctt attttcatca aatcgatcga ttcactattc cagaatgggt aaaagaaaca
    32461 gtatggtatc aaatttttcc cgaaagattt gcaaacgggg acaaagcaaa tgaccccgaa
    32521 cacactttac cttggggaag taaagatcca gaccgagaag acttcttcgg gggagatttg
    32581 caaggtgtga gggatcattt ggattacttg acggaccttg gcgtcaatgg catctatttt
    32641 tgtcccatat ttaaagccac atccaatcat aaatacgata cgattgatta ttatgaggtg
    32701 gatcccgctt ttggcgataa aaaactttta aaaaacttga ttgatgaagc acataaaaga
    32761 gggatacgta tcatgttcga tgctgtattc aatcatatgg gtgtccattc accacagtgg
    32821 caagacgttc tcaaaaacgg agaaaaatcg atctataaag actggttcca catccgtttt
    32881 ttcccagtag attcttatca gatgactgac ttaccagaaa ctgctgaaaa tatcccatat
    32941 gatacttttg cttttactcc attcatgccg aaactaaata cagcaaatcc agaagtgcaa
    33001 aaatatttgc tggatatcgc aacttattgg ataaaagaat tcgatattga tggatggcgt
    33061 cttgatgttg ctaatgaagt cgatcatcat ttttggaaaa aattcagaga agcagttaca
    33121 gaaatcaaac cagacatcta tatccttgga gagatttggc attcctctca agcttggctg
    33181 caaggggatg aattccatgc agtgatgaat tatgcattca cagattcgat taaagattat
    33241 tttgcaaaaa agaaaatcac agcttcacaa atggtcagtg gaatgaatca tcagcaaatg
    33301 ctttacagag atcaggtgaa cgaaggaaca ttcaacttat tagattctca tgatactgcc
    33361 aggatcttga ccctttgcca aggtaacaaa gaattgatga agtctgtgat ggcctttatg
    33421 tttcttcaaa aagggtctcc ttgtatctat tacgggacag aaatagggat gactggtgaa
    33481 gatgacccgg attgccgcaa atgtatgata tgggagaagg aggaacaaga tctagagcta
    33541 ttcggtttta taaaagaact tgtttctcta aggaaacagt tgtctaagat tatttctgaa
    33601 ggttctactc agtggctaat agtatatgat cgtgaagata agctctattt cacacgggaa
    33661 ctagagggac agatcatcta tgtttacttt aatcagtcaa aagaaccttg ggtagtagaa
    33721 caggaaaatg aggtgattct tagtcaaaat tgtcagttgt tagaagacgg gaaagcagag
    33781 atacgacaat acggattttt gatcatgtgt tgaaaactgt taccggtaac aacaagaaag
    33841 cgtttgcaaa tgaagatttg tgcccattat aataagggtg taacagcacg ttgaatacac
    33901 aggaggaaat gaagatgaaa aaaagaagtg tgaaacagtt gggtttaggg ttaatctctt
    33961 taggaatctt aggcggatta gcggcttgtg gcaatggtaa ctcttcagca gaaggggaag
    34021 ccgatgaagg aaaaacgtta acagtttccg ttgattctgg atataaagat tatataaacg
    34081 aaataaaaaa cactttcgaa aaagaaaacg atgtgaaaat caagctagta gaaaaggaca
    34141 tgttcgatca gttggaatca ttagcgttag atgggcctgc tggaaaggga cctgacgtta
    34201 tgatggcagc atatgaccgg atcggtgcgt taggacagca aggacatctg gcggaagtaa
    34261 aactgggaaa tgaagcagct tacgatgaaa cagacaaagc ccaagtgact tatgatggaa
    34321 aaatctacgg agaacctgca gtaatcgaga cccttgtttt gtactataac aaagatttag
    34381 tcgatacagc accatcaacg tttaaagatt tggagaatct atccaaagac agtcgttttg
    34441 cttttgagtc agaagcagga aaaaatacag gatttttagc taaatggact gacttttatt
    34501 attcctatgg tttaattgca ggttacggtg gctatgtatt tggtgatgat ggcacaaatc
    34561 cttcagatat cggtttgaat aatgcaggtg ctgtagaagg aatctcttat gcaacagatt
    34621 ggttcaaaaa tgtttggcca aagggaatgc aagacataaa aagtgcagga gacttcgcca
    34681 gccagcaatt catgtccaat aaaactgcag caatcattga cggtccatgg caagcgcaaa
    34741 catacaaaga aaacaatatc aattatggtg tagccaaaat tcctactcta aataatggac
    34801 agccttatca accgtttggc ggaggaaaag gatgggtagt cagcaattat gctaaaaaca
    34861 aagacctctc tcaaaaatgg cttgattatg taacgaatca ggaaaatcaa gaaaaattct
    34921 ttgaaatggt aaatgaaatc cctgcaaacc aacaagctag ggaaacagca aaggctaaaa
    34981 acgatgaatt aacaacagct gtgattgaac aatatgaaac agctcaagcg atgccaaaca
    35041 tcccagaaat gggcgaagtt tggacaggtg cagaaaactt gatgttcgat gcagcatcag
    35101 gttcaaaaac accaaaagaa tccgcagatg aagcagtaaa aacaatcagt gaagccatag
    35161 aacaaaaata ctgattaaaa tgataaccgt ccatttttat aaaaaatgga acaggtgctt
    35221 cagataaaag tggcaagagc gagatggaaa attatcttgc tcttgttgcc attatcttaa
    35281 aagcagtttg cggtttagca agtttagagg gggcattcag atgcagcaag ataaaacaag
    35341 caatatcaac gaaaaaaaaa tcacgctttg ttcacttatt ccagggatgg ggcagtttgt
    35401 aaatggccaa gcttttaaag gtatcgtttt tttcgccata ttcgtcctat tcattggtca
    35461 gatgcttctg ggcggatttc atgcattggt aggattggtt actttgggaa gtataccaat
    35521 ggaagatcat tcattattta ttttgattga aggcactttg caattcatca ttacaatcat
    35581 tttcttgatt ttttacggtt tgaatctttt agacgcttat cgagtagcga agatgcgtca
    35641 agagggcaaa aaaatcaatc ggacgatgaa ggaagtcata cataatatag gtgatgaagg
    35701 ttttccttat ctattgacac ttcccgctta tcttctcatg atttttacga tcattttccc
    35761 agtcctagtt acattgttca ctgcatttac taattatgac ttcaatcata ttccaccagc
    35821 cagtttaatt gattgggtag gggctaaaaa cttttttagt atcttctttt taagctcata
    35881 tcgtaatact ttttttgcag tcttttcgtg gaccgtgatt tggactttat gtgcaacaac
    35941 tttgcaaatc acccttggta ttctcacagc ggttgttgct caccagaaat ttattaaatt
    36001 caaacgtatt ttcggtatca ttttcttatt accatgggcg gtcccagctt ttattactat
    36061 catgagtttt tcgaatatat tcaacgatag cgtaggtgcc atcaatactc aagtgattcc
    36121 tttcataaac cagcttcctt ttgtagatat cagtgcaatc tcttggaaaa cggacccgaa
    36181 ctggacgaaa gtagcaatta tcttagtgca gggctggtta ggatttccct atatctatgt
    36241 gatggttaca ggaatcttac aatcgatttc agaagactta tatgaagcag caaaaatcga
    36301 cggagcaaat gcatggcagc gcttttcaaa catcacgctt cctgctgtct tcctagtggc
    36361 agcgccaaca tttattacac agtatacggg gaatttcaat aatttctcca tgatctatct
    36421 gtttaacaat ggtggtcccg gaagcgtagg cggaggagct ggttctactg atattcttat
    36481 ttcctggatc tataaactta cgactggaac ttctccgcag tattctatgg cttcagcaat
    36541 cacgttgatt atctccgtca tcgttatctg tgtgtcattg ctggtattta aaaagacaaa
    36601 tgcgttcaat atgggggatt aacaatgaaa aaatttcatt cgagtgctcg attccaaaaa
    36661 cgactttctc acttttttac ttactttttt cttttaatac tgtcagtcat tatcatctat
    36721 ccgcttttga tcacagcaag tactgccttc aagtcaggaa atattgcagc ttttagttta
    36781 gatctttcaa gtgactggac tttaggaaac tttactcggc tattcagtga aacactttac
    36841 gggacttggt ataaaaatac attgatcatt tctgtcctaa caatgatcgt acaagtgact
    36901 gtagtaactt tggcgggcta tacttatagt cgctatcgtt ttatcggccg caaatcaagt
    36961 ttgatgttct ttttagtgat tcagatggta ccgactatgg cagcattgac tgccttttat
    37021 gtcatggctt tcttattaga agcgctcgat aagtactggt tcttgacaat gatctatata
    37081 ggcggtggaa ttccaatgaa tacttggctg atgaaaggat actttgacac tgtaccaata
    37141 gatttagatg aatctgctaa acttgatggg gcagggcatt tcaggatttt tttccagatt
    37201 gtattaccgc ttgtcaaacc gatgctcgcc gttcaagcat tatgggcttt tatgggacct
    37261 tttggggatt tcatgttagc taagttcctg cttcgaacac ctgaaaatca gactgttgct
    37321 gtcggcttac aaacatttat ttctgatgtg acaaaccaaa gagtctctct ttttgctgct
    37381 ggggcaattt tgattgcact accgatttgt ctattattct tctttttaca aaagaacttt
    37441 gttaccggcc tgacatctgg cggaacaaaa ggataggagc agagtatcat gcaaacattt
    37501 ccgttaaact atttttccgc attacgtacg ccaactcaag tgtttgcggg acgcaaacta
    37561 ttatcatggc ctaaattttt cttgatattt gtatttttag tttccttgat ggtcatgcct
    37621 gtgactcttt tttatgccaa tcagatccaa gcgattccat tagaacaatt tctatccgtt
    37681 cattcgctga tcgatgaaca aggaacccaa aaattttctg aacttgaatt atctgaaact
    37741 gggttacaag catcgcaaca aacgatagct gtgacccctg aaattttggt aggtgtgtct
    37801 ttatctgaga aacaacaatc cgatcatgga acttttattg attttgagaa ggaacaatgg
    37861 gtgatccaac aaaaggataa atctggtata cgccgctata cgatgaatta tagcccatct
    37921 ttccaaccag attctgtacg tacgcccgaa gatttccaac gatttttaga aagagaattt
    37981 tatgccagta atcgtccaac aatcatttta tcttatagtt taagtcttgg tcttgtccta
    38041 tttgtcatga cgagtctcat tttattcggg gcttcctttt tcttgtggat gacaagaaaa
    38101 agccaacttt catccattca tacattcaaa gagagtgcaa atttgatgct gaatgttatg
    38161 ggaataggtt ccatggcagc agcagttgta gggtttatcc actttgactt tatcttgatg
    38221 ctaagtgtcc aaacaacgat aactgtcttg ctgcttttat gggtttttgt caaaacaaga
    38281 ttcaaagata agcgtgtcga atgacaagtt taagcgattt atgcgacaat gaatgtgaaa
    38341 aaaatgaaaa ataaagtagt ttttgaaaaa tttctgaaaa aattaaactt cttagttgat
    38401 tcttttcttt tttagtatta acgtaatagg gtagtttttt taaggaggaa aatcatgtta
    38461 gagaaaactt tatacaatca attattttca cgttctttct cgctccctgt cgaagtaaat
    38521 tattgggacg gtacaacgaa acaatacgga gatacggaca ctcctcccca aatcaaaatc
    38581 aatatccatg aagaaatccc aatgaaagag attacaagta acgcttcttt ggcactagga
    38641 gaagcctata tggatcatcg aatcgagatc gagggaagta tccaagcatt gatcaacgat
    38701 gcttatcagc aagcaaacag ctttttacga ggaaaagatt atctaaaatg gctgccaaag
    38761 aaagaaaaac atacgaaaca aaaaaacaaa gaagatatcc atgcgcatta tgacttaggt
    38821 aacgactttt ataaactttg gctagatcca actttgactt attcttgtgc ttatttcaat
    38881 acacctgaag atacgttgga acaagcacaa atcaataaag tccatcatat cttagacaaa
    38941 ctctttatca aagaaggcga aacgttgctt gatatcggtt gtggctgggg aacattgatg
    39001 ttcactgcta ccaaagagta taatgtcaag gcaactggga tcacactaag tgaagaacaa
    39061 tacgatttga ttaccaaaag aataaaagaa gaacatttag aagataaatg ccgtgtatta
    39121 ttaatggatt accgcgaact aaaaggcgaa acgtttgatc atatcacaag tgtaggtatg
    39181 ttcgaacatg taggttctga aaatcttgaa ggttatttca aagtggtaaa agatttattg
    39241 aaaccaaaag gaacagcttt gatccatgga atcagccgac aacaaggcgg tgcgacaaat
    39301 gcttggatca ataaatatat tttccctggc ggttatgtcc ctggtgttac tgaattagtt
    39361 gataatatta caaaaaacga tttgcaactg attgatttgg aaagcttacg tcgtgactac
    39421 caattgacat tagaacattg gacaaagaat tttcatcaag tccaagatca agtcacgaaa
    39481 gaaatgggtg attccttcta tcgaatgtgg gatctatatt tacaagcttg tgcagcttca
    39541 tttgaatcaa gtaatatcga tgtgattcaa tatttgcttg ttcaacctgg aaacaatgac
    39601 atcccaatgc atcgtgcata attgaggcaa aagtactgtt taaagactat ttaaaaagaa
    39661 gctaagtttt atgatgctcc agaagtgaac ttttttgtct aacttctagg attgtctcta
    39721 aagcttagtt tctttttctt tatattttca taataaaagt ttggttcttt aatttttaaa
    39781 catgacagaa aattttttct tcgttatact tgtaatagaa tactatttta taacaaaggg
    39841 gatcgaatca atgaaaaaaa ctgttattta tactagttta tttgcagctt tgtttttagc
    39901 tgcttgtcaa tcaaatacca atcaaggcac atctgggaat acgtcttcca gtagcgaaca
    39961 gcgctcttca caggtatcgg actcctcaac aaaagcaagc acatcttcgt ctagccagac
    40021 aaaaagttct agtgaagcaa gtactcagtc agatatcaaa gtgtctgcag ctcgtgcgat
    40081 cgaattgttc caagataaat acccagatac tgcaattact agtttggaat tagatagtga
    40141 ttggggatct tacttctaca agatagaagg tgtcgatgat caaaaagaat acgaagcaag
    40201 aattgatgct caaaatgaaa aaatggagac agagaatcca gaaactctag atcgagatga
    40261 acaaaatggt caaaagaaaa aagaagatgg tctggatgtt gataaattga tcacgattga
    40321 ggaagcagca aaaatcgccg tcgacaaagt aggaagcggt acggcaacag attgggattt
    40381 ggataaagat ttaggtacta cttattggga agtcaaagta aaagacggca atcaaagtac
    40441 atcagtcaag atcaatagcc aaacaggcga agtactgtca actgaacaag atgattaaga
    40501 atttcttcgg aaaacaagag ttgatttttc ttttagtcat gatacactag acaaaataac
    40561 ttgtttgaag gagcgattta cttgagttta gaaacgacct ttgccaatca tatctttgcc
    40621 aatcctttga tgaatgcatc tggtgtccac tgcatgacta cccaggaatt agatgaactg
    40681 gctcattcag aagcaggagc atttatcact aagagctgta cgatcaatga acgaaaagga
    40741 aatcccgaac cacgctattt tgatgttcct ttagggagta tcaattctat gggacttcca
    40801 aacttaggtt tttcttatta tctagagtat gctcttgcgt atgaaaaagt gcaggaaaac
    40861 caaaatcaac cgttattttt ttccattgcc gggatgagtg tccaggaaaa cttggaaatg
    40921 cttgagaaaa tagaaaaaag tgggtttaat gggatcactg aattaaattt atcttgtccc
    40981 aatgtgcctg gaaaaccaca actagcttac gattttgagg ccacttatga aacactgaaa
    41041 gaagtattca gcatcttttc taagccgcta ggaatcaaat tgcctcctta ttttgatttt
    41101 gctcattttg atcagatggc tgatatcttg aatcagtttc cattgaccta tgtcaatgct
    41161 atcaacagtg tgggcaatgg tttgtatatc gatacagaac aagaagcagt agtcatcaaa
    41221 ccaaaagaag ggttcggcgg cattggcgga gagtatatca agccgactgc tttggcgaat
    41281 gttcgcgctt tttatacacg tttgaaacca gaaatccaaa tcattggtac aggtgggatt
    41341 cggacaggac aagatgcatt tgaacatctg ctatgtggcg caagcatgtt acagattggt
    41401 acagagcttc ataaagaagg gccggaaatt ttttctagaa tcataaaaga attaacgcag
    41461 attatgtcag aaaaaggata tacgtcaatt gacgaattta aaggcaaatt acgcaccatt
    41521 tcatagcaaa gagacgaagg tcatgattga tcttcgtctt tttttataaa aaacactcgt
    41581 ctgttcattc ctgtgcaagc gtactcattg tatgttagac gaacttgtgg gaaaataaca
    41641 gaggcaaaaa ggaggataat atgaatgatg caacagaatt agcaaaagag cttaaagaaa
    41701 aagaatttag ttttcaagaa ctagttacgg atatacagaa aaaaataaag aagcaaaatc
    41761 atgaattgaa tgcgtttgtc gcgcttgaac cattcgagag taattcccac gagctattga
    41821 ttccccagtc agaaaaaggg ccattggctg ttatcccatt tcctttaaaa atgctcggtc
    41881 aagaaaaaaa gggatggcta gctacttcag gatctcgttt atttgaaaca caccgagctt
    41941 ctcatacatc taattatgtg cggcaagcag aagcgatagg tctagttcct tttggacaaa
    42001 ccaatgcgcc agagtttggt ttcaaaaata ttacagaccc agtcatctat ggcccagcaa
    42061 gaaacccttg gaaccttgaa tgtacaccgg gcggatcaag tggaggagct gctgctgcag
    42121 tttcatcagg aatcgttcct ttagctggtg ctagtgatgg cggtggttct attagaatac
    42181 ccgcttcgtt ttgcggattg attgggctga aaccttcgcg agggacgatg ccagttggtc
    42241 catatgcttg gagaggatgg caaggagctg ctattgattt cggcttgaca gtttctatga
    42301 gagacacgga ggctttattt tatgggatga gaagtatcca ttccggtgcg ccatatcaag
    42361 tatcgccagt tgagtggcaa acacatccaa gaaaaaaacg gttgaagata gctttttgtg
    42421 ttgattcacc gatccactca cagattagtg aagaggcagt acttgctgta aaaaaagctg
    42481 tagatttcct tggaaaagaa gggcatgatt tgacagagat tccttaccca ctagatggtc
    42541 gtaaactgat cacgtcttat tatcagatga atggcgcaga aacagcagca atgatccgct
    42601 cgattgaaac aggcatccag cgagctgtca gaaaagaaga acttgaaccc atttcttgga
    42661 ctttgttcca atatggtcag aagctctctg ccgctactta catcgattct ctgcatgtat
    42721 gggatgaagc agcaattgtc atggaaaacc tacatcaaga atacgatcta ttcctttcac
    42781 caacgactgc atttaccgcc cctaaaatca gcgaagattt gcaaagtgat gcgataagag
    42841 cacagatgga acacatcagt gattgttctg aagtagaagg tttagacgtt gtttcttcca
    42901 tgtttgagga gagcttgaag atcacacctt atacacagtt ggctaatctg actggacaac
    42961 cagcaatcag cttgcctact catttgacaa aagaaaacat gcctctagga atccaattta
    43021 tggctgccag aggaagagaa gatttgctat ttcaaatcgg ctatctattt gaagcaaatc
    43081 atcagttcca tttaccaaat ccatggaaat agcagaaaca aggagaagaa gaatgaagaa
    43141 aaaaagattg tttgctctaa taactagtgc cttttttatt ttaagcgctt gcggtacagc
    43201 tgccgctaat caggaagata acagctggag taatgtagaa aaaaagaaag aattagtagt
    43261 agcgacttct ggcacactgt ttcccgcatc ttattataat gatcaaaatg aattagttgg
    43321 ttatgatgta gatttggcaa aagaggtggc taaacggtta aatgtcaaaa tctcctttaa
    43381 agaatacaac gtggatggac aaatcacttc tgtttcaaaa agagaatcag attttgctgc
    43441 gaacgatttt ggtatcacaa aagaaagaga aaaaaaattc tccctttcag agactttcaa
    43501 atattcattt gacagtatga tcgtacgaaa aaaagatgat tcaggaatcc actcacttga
    43561 agatcttaaa gggaaaaaag cagctggcga acccaatact agctatatgc gtttagcgga
    43621 aaaacacgga gctaaattag tcacatatga caatgccacg aatgatcagt atttaagcga
    43681 tgtagcaaat ggtcgaacag atgttattct aaatgattat tatctgcaga aaatggccgt
    43741 atctgcttta ccagatatcc ctgtaaaaat actggagaac gtttatttca atgaaaacga
    43801 aagtggtttt ttattgaaag aaggaaatga tgctctgaaa aagaaaatcg atggcgtatt
    43861 aaaagagatg aaagaagatg ggacgacaaa gaaaatatct gaaaaatatt ttcaaactga
    43921 tgtttcagtc aaaccgaaag aaacgatggc tgagtcggtg agtgagtgat gtactttcct
    43981 ctaattgatt tttccctcat gtggacttca cttcctttcg tactgcaagg cttggtatac
    44041 acattaggga ttggcttcgt gtcgtttctt ttaggaaatt tggtagggtt gcttttgaca
    44101 gttttaggtc ttttggattg gctgcctctg aatgtattta ttcgttttta tttatcgttc
    44161 tttagaggca ttcccgcact agtcctgctg tttctgttat atttcgggtt accttatcaa
    44221 ttaagtgcct taacggcttc agtcatctgc tttacgatta ccagcagtgc atttattggt
    44281 gaaatctatc gaggatcact agcaggcgtt tcttcaggtc agtgggacgc tggtttagct
    44341 tctggttttt ctttctttgg ggtattgaaa tatatcgttt tgccgcaagc ttttcgaatc
    44401 tcagtaccgg cactcagtaa cgtagctatg gatctagtga aaggtacttc gttagcagca
    44461 acaatcacag tgccagaaat atttcaaaaa gcaaagatca taggcggtcg gacattcgat
    44521 tatttttctc tctatatttt agttgcactg atttattggg tcatttgtat cgggatcgaa
    44581 aaagtgcaac agtatttaga aaa
//
LOCUS       DACEUF010000011        43921 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.012, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000011 DACEUF010000000
VERSION     DACEUF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.012"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(137..1528)
                     /locus_tag="IXG20_000626"
     CDS             complement(137..1528)
                     /locus_tag="IXG20_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ1608842.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1741..2127)
                     /locus_tag="IXG20_000627"
     CDS             complement(1741..2127)
                     /locus_tag="IXG20_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ1608843.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2183..2806)
                     /locus_tag="IXG20_000628"
     CDS             complement(2183..2806)
                     /locus_tag="IXG20_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ1608844.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2870..3538)
                     /locus_tag="IXG20_000629"
     CDS             complement(2870..3538)
                     /locus_tag="IXG20_000629"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1608845.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3550..3990)
                     /locus_tag="IXG20_000630"
     CDS             complement(3550..3990)
                     /locus_tag="IXG20_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608846.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4049..4720)
                     /locus_tag="IXG20_000631"
     CDS             complement(4049..4720)
                     /locus_tag="IXG20_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1608847.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4905..5303)
                     /locus_tag="IXG20_000632"
     CDS             complement(4905..5303)
                     /locus_tag="IXG20_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ1608848.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5300..6073)
                     /locus_tag="IXG20_000633"
     CDS             complement(5300..6073)
                     /locus_tag="IXG20_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1608849.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6070..6753)
                     /locus_tag="IXG20_000634"
     CDS             complement(6070..6753)
                     /locus_tag="IXG20_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1608850.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6920..7780)
                     /locus_tag="IXG20_000635"
     CDS             complement(6920..7780)
                     /locus_tag="IXG20_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1608851.1"
                     /translation="MERPNVKKQALELRSTIGSVLKYTALSILSFISIFPFVWMVLGM
                     TNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVFALLISSMAG
                     YGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINSFLVVILPSV
                     STAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAAAAIITFMSS
                     WNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTAIVFFVLQKQ
                     FVQGMIGSVK"
     gene            7761..8684
                     /locus_tag="IXG20_000636"
     CDS             7761..8684
                     /locus_tag="IXG20_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1608852.1"
                     /translation="MTLGRSIVSFSTFSEESGYVINTDSKDTLRKFVQKFRQEVFPGD
                     FFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSYLT
                     KIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEELSL
                     LKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYEKE
                     INLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEICEK
                     VGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            8681..10417
                     /locus_tag="IXG20_000637"
     CDS             8681..10417
                     /locus_tag="IXG20_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1608853.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(10480..11316)
                     /gene="pheA"
                     /locus_tag="IXG20_000638"
     CDS             complement(10480..11316)
                     /gene="pheA"
                     /locus_tag="IXG20_000638"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ1608854.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(11313..11822)
                     /locus_tag="IXG20_000639"
     CDS             complement(11313..11822)
                     /locus_tag="IXG20_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ1608855.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(11815..13110)
                     /gene="aroA"
                     /locus_tag="IXG20_000640"
     CDS             complement(11815..13110)
                     /gene="aroA"
                     /locus_tag="IXG20_000640"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1608856.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(13123..14214)
                     /locus_tag="IXG20_000641"
     CDS             complement(13123..14214)
                     /locus_tag="IXG20_000641"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ1608857.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(14231..15397)
                     /gene="aroC"
                     /locus_tag="IXG20_000642"
     CDS             complement(14231..15397)
                     /gene="aroC"
                     /locus_tag="IXG20_000642"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ1608858.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(15399..16463)
                     /gene="aroB"
                     /locus_tag="IXG20_000643"
     CDS             complement(15399..16463)
                     /gene="aroB"
                     /locus_tag="IXG20_000643"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ1608859.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(16496..17518)
                     /gene="aroF"
                     /locus_tag="IXG20_000644"
     CDS             complement(16496..17518)
                     /gene="aroF"
                     /locus_tag="IXG20_000644"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ1608860.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(18177..19151)
                     /locus_tag="IXG20_000645"
     CDS             complement(18177..19151)
                     /locus_tag="IXG20_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ1608861.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(19132..21015)
                     /locus_tag="IXG20_000646"
     CDS             complement(19132..21015)
                     /locus_tag="IXG20_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ1608862.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(21033..23258)
                     /locus_tag="IXG20_000647"
     CDS             complement(21033..23258)
                     /locus_tag="IXG20_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ1608863.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            23373..24407
                     /locus_tag="IXG20_000648"
     CDS             23373..24407
                     /locus_tag="IXG20_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1608864.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(24474..25487)
                     /locus_tag="IXG20_000649"
     CDS             complement(24474..25487)
                     /locus_tag="IXG20_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1608865.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(25565..25753)
                     /locus_tag="IXG20_000650"
     CDS             complement(25565..25753)
                     /locus_tag="IXG20_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ1608866.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(26193..26321)
                     /locus_tag="IXG20_000651"
     CDS             complement(26193..26321)
                     /locus_tag="IXG20_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1608867.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(26324..27697)
                     /gene="rlmD"
                     /locus_tag="IXG20_000652"
     CDS             complement(26324..27697)
                     /gene="rlmD"
                     /locus_tag="IXG20_000652"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1608868.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(27808..28863)
                     /locus_tag="IXG20_000653"
     CDS             complement(27808..28863)
                     /locus_tag="IXG20_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1608869.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(28873..30303)
                     /gene="gatB"
                     /locus_tag="IXG20_000654"
     CDS             complement(28873..30303)
                     /gene="gatB"
                     /locus_tag="IXG20_000654"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ1608870.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(30300..31772)
                     /gene="gatA"
                     /locus_tag="IXG20_000655"
     CDS             complement(30300..31772)
                     /gene="gatA"
                     /locus_tag="IXG20_000655"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ1608871.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(31772..32077)
                     /gene="gatC"
                     /locus_tag="IXG20_000656"
     CDS             complement(31772..32077)
                     /gene="gatC"
                     /locus_tag="IXG20_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ1608872.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVTETEDGFIKVPAIIDNGEAGA"
     gene            complement(32183..34219)
                     /gene="ligA"
                     /locus_tag="IXG20_000657"
     CDS             complement(32183..34219)
                     /gene="ligA"
                     /locus_tag="IXG20_000657"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ1608873.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(34236..36470)
                     /gene="pcrA"
                     /locus_tag="IXG20_000658"
     CDS             complement(34236..36470)
                     /gene="pcrA"
                     /locus_tag="IXG20_000658"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ1608874.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDLEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            36625..38184
                     /locus_tag="IXG20_000659"
     CDS             36625..38184
                     /locus_tag="IXG20_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ1608875.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(38295..40226)
                     /locus_tag="IXG20_000660"
     CDS             complement(38295..40226)
                     /locus_tag="IXG20_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1608876.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(40244..41158)
                     /gene="pfkB"
                     /locus_tag="IXG20_000661"
     CDS             complement(40244..41158)
                     /gene="pfkB"
                     /locus_tag="IXG20_000661"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1608877.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(41155..41907)
                     /locus_tag="IXG20_000662"
     CDS             complement(41155..41907)
                     /locus_tag="IXG20_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1608878.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(42067..42276)
                     /locus_tag="IXG20_000663"
     CDS             complement(42067..42276)
                     /locus_tag="IXG20_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608879.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(42287..43615)
                     /locus_tag="IXG20_000664"
     CDS             complement(42287..43615)
                     /locus_tag="IXG20_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608880.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT        12185 a         9504 c         7862 g        14370 t
ORIGIN      
        1 caacctctta ttcacggtgt ttcagaagta gcttctcgga aataagccga aatttcacaa
       61 aaatttgaga aactattttt aggaaacttc ttcttatttc ttaaagctga ccacttctat
      121 cacaacctct ttttgactat gcattcacat cgatcacttt agaatcatca ttgatgacat
      181 cgcgatctaa atctttgatg cgtgcacttg tcacgatacc agctaccatg ctaccgctga
      241 cgtttactgc tgtacgtgcc atatcgatta atggttcaac agagataact aacccaacga
      301 ttgctactgg caagttcatt gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac
      361 cgactccagc tacaccaaaa gaactgattg ttacaattgc cacaagcatc aaaataaatt
      421 ccaaactaaa gacattcact ccagctgctg gtgcgacgat cgttgccaac atagctggat
      481 aaacgccagc acagccgttt tgtccaatag acaaaccaaa acttgctgag aaattagctg
      541 ttgcatcatc tactcctaaa gcttttgtct gagtctcgat attcaatggc aaagctccag
      601 cacttgaacg tgaagtaaac gcaaagctca ataccggaaa ggcttttttg aaatagttga
      661 ttggattgac ttttgcacca atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg
      721 ctacgtaaga agcaacgaca aatttaccta aattgacgat agctgcaaaa tcacttgttg
      781 ccaaaacgtt tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa acaagcgtca
      841 cgatccgcat aatgatttta tacagactgt cgactaaatc tgaaaagaat gctgcttctt
      901 ttggcgcttt tcttttcacg cctagataag cgattccgac aaatgccgcg aaaataacga
      961 ctccaatcgt acttgttgca cgactacctg ctaaatcagc aaaaacgttt ttagggataa
     1021 aggataaaat ctgctgtgga atcgataagt tttcaactgt ttcctgacgt gttgccagtt
     1081 cagagattcg tgctgtttct gcagctcctt gtgtgaattt agctccttgg agtccgaaca
     1141 gcattgttga accaattccg atcaatgctg caatagctgt tgttccaagt aaagaagtca
     1201 atacagtcgc actaattttc cctagctttt cagaaacttt catttttgtg aatgcaccaa
     1261 tgatcgaaat aaacactaaa ggcataacta gcatttgcaa gaatgataca tatccttgac
     1321 ctacaatccc gatccattcc attgattgag taacgacttt gtcgtctaca ccaaagatca
     1381 attgaagcag accgccaaag acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa
     1441 attttgtatg gcgcttttgc atacgataga acacatataa taatgccgca aaaactagaa
     1501 ccactaaaac tgtgatgagt gttgtcattt ttctcctcct catattatta atgacaagtc
     1561 catcgaagga agtaaaggtt cccgagaatg attcttcaac catcctcctg aaaaaatttt
     1621 tttcagtgac ttttatattt tagacgattt ttatcgaaat gacattcttt ttgcttacaa
     1681 ttagtaataa aaaaacatac ctgtgatagc tttaatctat cgcaggtatg tttttactgt
     1741 ctattcttgt ttttctgatt gaaaattcaa ttttacccca ggctgatcta aggcgatctc
     1801 tgtcacctca taagacattc ggcgtacgat atcgaataac ggtgctacta gctcgtccac
     1861 ttcttcttgt gtgacatcat tttgaccttc aattttccga ttgaggatat gattgatctg
     1921 actcacactt ccagataaaa catatgtatc aaacactaaa cggaactcta agcgtgctcc
     1981 tacgatgcta tgttctttcg gatagttttc gatcgtttct tttaaaggcg agaagcctac
     2041 tttcaaatct gtttctactt cttggttcgg caatctcata tcataatgaa atgcttctac
     2101 aacttctttt tgtcttctga tttccatata attttatcac tcctttatga aaattataac
     2161 atatctcctt tgaaagggga tactactttt taatgatgct ctgcaaggta aggatttcat
     2221 tcagatttcc taccacataa tctgccggga tctcacccag aaaatgatcg atgtcgtaaa
     2281 gacaagtagc gactcctgca tttttccctg cttcgatatc taaacgacga tcgccgatca
     2341 tcatcgtgtc tgttctttct aaatgaaaag tatcgatcaa ataatttaaa gatgctggat
     2401 caggtttacg aggaaagtcc tgatcgatcc ctactacttc ctcaatcaga tgtgccaatc
     2461 gatgttcttt taacaggccc catgttgatt ctgtcaaacg gtgggtcagg atgaaatgac
     2521 gaccgccgtt gtcttttagc atttccagta cttgtttcgt ttctggaaat ggacgggaaa
     2581 cttccgtact ttctttttca tatcggtgaa ataattcatt aaactctctg gcattcaagt
     2641 ttgattcatt gatcaaataa ctagtagaat actttttcat tttgaagtag atttcttttt
     2701 tgtccatact gataccaaaa tcttttagtg cttgccatgc accatctacc attgcgggat
     2761 acgtatcaaa caatgtacca tcaaagtccc atatataatt tttcatgttc cttttgttcc
     2821 attcctttgc ttcttttttc tatctctcac ttttagataa aggtgttctt taaagtattt
     2881 ttttgtccag aattgtcaaa tcatctgcta atgtatactg gattggttgt gcatttggga
     2941 tatcgatcgt atccatttga tcttcaggaa catcttctaa atattttgtc agtgctcgga
     3001 tgctattacc atgacccaca actagtaaat cttgccgatc ttttagtttt ggtgctagat
     3061 gatcttgcca taagggaatg atccgattca ctgtcatttc cagactttct ccgtatggga
     3121 tcaaatgcgg atccaagtga tcatatctgc ggtcaaatct attttctgcg gttaacggcg
     3181 gaacttccgt atatcctctg cgccatgttt ttacttgatc ttttccgtat atttctttca
     3241 tcttatcttt gtttttaccc acaagatccc cataatgccg ttcatttaga cgccatgttt
     3301 ttattatagg aagccataac tgacccattt cttctagtgc gacttgtccg gtaagaatcg
     3361 cacgtttcaa aacagaggta taaacggcat cgaattgaag tccggcttca agcatttttt
     3421 gaccggcaac tgctgcttgt tcttttcctt tttctgttaa ggcgacatct agccatcctg
     3481 tccagtaatt ttcaaaattt gcttcgcttt cgccatgacg taataatact aatttcattc
     3541 ttttctccct taaacattcg tttcgaattt tataccacat gttttgcatg tcacacgtat
     3601 tcgtcctttt cctttaggtg cacgctgctt ttgatgacaa ttcgggcact caaaaaaagt
     3661 ataacttttt cgatccattt tgacttgctt tttgtttttt atctgatgat atttttcttt
     3721 gactctatca agatatttca aataacgttg attctcattt agtctggggt agattttttt
     3781 tgataggaaa cgaaatatca tccatccgaa aaagacgatg aacaacaatt gtccgattct
     3841 atagggcaac caacgactta gcaaactaaa gacaagcgca gcgatcaata gaccgcgatt
     3901 caactgatca aatttcgcat atcttccctg cataagtttg tgatagttct gctgaaatcg
     3961 tattagcctt tctaaccatg tttgcatcat attatctcct ccatcatcta atatttatta
     4021 tgacaaaaaa atccaaaaag aaaaagcctt aattggcctt ttccattgca acttggtgaa
     4081 ggacattccg atttctggta aagtcaatca caatcacttt ctttttatct tctctgttga
     4141 ctactcgaaa tgtgtccgtc aatggaagaa acagcttctt agatggtagc tgttgcatac
     4201 gtataaaacg atggatcatc gtaatcaatt gaggaaattg aatagacgtg tgaagcagac
     4261 ctggatattt tttcaacagc tgtgtcgttt tgatcagatt ccgtttttta ctgatcactt
     4321 ttaaaaagct ttttagaatc ttttgctgac gtctaaaaat agaaaaagaa ttttcgtcat
     4381 ctatttgata acgcatttgt ttttgcagtt ctgctgcagt cagatggatc ttcccatcgt
     4441 ttgtatcgat cattaaacct tctggaaaca gttcaaccac tgctcgatcc caagattcaa
     4501 gagaaagctc gatataatct tttactggta attccaattc taccttaaaa aagtgcaaaa
     4561 cagccgacag cccttctgct tgatagattt ctcctaatgt tagcccctct agatggaact
     4621 ctggtgaaaa atgcagcaat gttttatttt cctgcaattg aacatataga aatgtagatc
     4681 gctcaatcag agtatctttt aaaacaacta aaaaattcat ttttctcccc cttttttcat
     4741 catttttaaa atagcatatt ttgaaaagaa agtctgatgc ttagagtaga atttcataaa
     4801 aatttactat ttcataagta ttcacgagaa aaatacaaac aaaaaagcgg aaataacgct
     4861 catccgcctt cttgtttgtg tttatttcct cttcaaacgc ttgtctatga ttcagacttt
     4921 tctctatcgt tattttcatc taaaagttct tgcattccga taatttctcg atattgatga
     4981 atcttagtga taccaatgac tttgtctaca tcaatactaa aacaaatacc attacctgga
     5041 atatcgatgt tgatgccttg accaatcgca tccattactt tattcgtcaa actatcgggt
     5101 actaatgtca acacaatatc tttttctggc gaaatgtcca aacctaaaaa tttccctgaa
     5161 tcatgtacac cagaacctct tccatgtaag atcgttccgc ctgctgcacc tgcttctttt
     5221 gaaaagtcga taacatcttc gcccattcct ccgtcaacga tcgtcacaat catgtgaaga
     5281 ttcaatgcta caagatcttt catttgcttt cccccaattt ttgcaatctt tctttttcga
     5341 ttctgtcttt ttcatctttt cgtttataaa tatatcctag tactaaagtg gataatattg
     5401 gtgtcaatgc aatcattgca accacaccaa acccgtcaat gatcggatct cttccttcaa
     5461 tttgtgcagc gattgccacg ctcattgata aaataaatgt cgaacacatc gggcccgttg
     5521 ctactccacc gttatcaaaa gccattgcta aaaagatctt gtccactttt cgtcctaaaa
     5581 taaatgctag cccgtatcct gggatcaaga aataataaag agagaatccg acgagcaaac
     5641 gccacataga caatcctact gctgcgccga tcccaattga aattaccatc agcatgattt
     5701 tcgatcggat tctcccctcg ctcaattctt caacctgttt gatcattaca tgaatcgccg
     5761 gctcagcaaa ccctactaag aaccccatta aaaagccaag gggaaccagt atataattgt
     5821 aaggcaatcc cgcaatagca ctgcctaaat gctgtcctac cggtacataa gccatattga
     5881 ctccgtgcag gaaaaggatc aacccaatag tcgttatcaa gaaacctttc ataatgtcaa
     5941 agaaacgctt tttgcgcata cgaaaactaa tgatattcat taaaacaaat aggattacga
     6001 taggtgccac tgccaaaatg acttccatgg taacgatttc gattccatct atcacttgct
     6061 gccagaaact cattggaaga tcacccccaa aatcatcacg gcaagaatcg gccctaacga
     6121 agcaataccg accattccaa aactgtcatt ttcatttttc ttttgtttga tcatactagt
     6181 catccctccg gcaagggcca agataaatgg aacagttact ggaccagtag tcactccccc
     6241 cgaatcaaaa gcaatcggca taaattcgga actggtgaaa aatgaggcga tcaaaacgcc
     6301 tatgtagcct atagccatca attgataata ggatagcttg aatacgaccc gaagcagcgc
     6361 aaaggctaga aatactccag tgccgacgct gacgatccca atcagcaaca ctctgccaat
     6421 ctctccgtca gatatatcat atacttgttg tcccaaaact tgcacggaag gttcagcaat
     6481 cgtgatcacg atcccgatag caaatcccaa actgaccaaa acggtcaaac ttttcttttt
     6541 gatcatatat ttccctacta gatctccgac ttccatcata gaataatctg ctccaaacaa
     6601 aaagagcgtc attccgatca tcatgattgc cgcaccaacc agaaatgaag cgaggtcttc
     6661 tccttctaac ggtgcaaaaa caaacgttaa aatgacaatc aggacagtca tcggcaatat
     6721 agcagcaatc acttctttaa aattttttag catgtatcct cctcctttac tctttaagtt
     6781 taccttaaaa tgaaagagtt ttcgagcaca aacttaaaac gttatcattt aatttttctc
     6841 aacataaaat aacacacgtc ccaaaagtat ctttttactt ggaaaaatga ctttgaagga
     6901 acgcgcttat tgtgtttttt tattttacag agccgatcat tccttgaacg aattgctttt
     6961 gcaaaacaaa aaatacgatg gcagttggta aagtagcgat gactaatcca gccatgatca
     7021 ttccatagtc tggtgtatag gaagcaccca tagcagataa aacaagcgga accgtccgtt
     7081 tatcaggaga ttgcaaagct actaagggcc ataaataatt gttccagcta ctcatgaatg
     7141 tgatgattgc agccgcggca taggtatttt tagccgtcgg catatacact ctaaaaaaaa
     7201 tacttagttc ttttagaccg tctaatcgag cggcttctac tagatctttt ggaaaagatt
     7261 taacattttg tcgaaagaag aagatcaaaa aagcagtgct aacagatggc aggatgacaa
     7321 ccaagaaact gttgattccc aaaaccgttc cgttcaattg tgaaaacatt cgatataaag
     7381 ggatcatcaa tgccgcaaaa ggaaccatca tcgatagcag tagaatattg aaaattaggt
     7441 cttttcgttt actttgataa atctcaaatc catatcctgc cattgacgaa atcagcaatg
     7501 caaacacagt ggtcaccaaa gcgatcatcg ctgaattcca aagggaacga gtaaaattca
     7561 gctcattgga aaacaaattt tgaaaattga tgatcaagtt actgccaatc tttatttttc
     7621 ccgatgtgat atccacgggt gtattcgtca ttcccaaaac catccagaca aaagggaaaa
     7681 tggaaataaa tgataggatg cttaatgctg tatactttaa tactgaccca attgtcgaac
     7741 gaagttctaa tgcttgtttt ttgacgttgg ggcgttcgat tgtttctttt tctacctttt
     7801 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     7861 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     7921 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     7981 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     8041 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     8101 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     8161 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     8221 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     8281 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     8341 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     8401 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     8461 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     8521 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     8581 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     8641 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     8701 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     8761 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     8821 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     8881 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     8941 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     9001 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     9061 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     9121 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     9181 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     9241 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     9301 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     9361 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     9421 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     9481 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     9541 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     9601 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     9661 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     9721 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     9781 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     9841 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     9901 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     9961 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
    10021 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
    10081 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
    10141 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
    10201 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
    10261 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
    10321 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
    10381 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
    10441 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
    10501 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
    10561 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
    10621 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
    10681 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
    10741 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
    10801 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
    10861 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
    10921 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
    10981 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
    11041 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
    11101 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
    11161 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
    11221 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
    11281 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
    11341 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
    11401 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
    11461 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
    11521 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    11581 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    11641 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    11701 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    11761 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    11821 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    11881 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    11941 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    12001 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    12061 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    12121 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    12181 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    12241 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    12301 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    12361 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    12421 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    12481 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    12541 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    12601 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    12661 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    12721 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    12781 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    12841 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    12901 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    12961 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    13021 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    13081 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    13141 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    13201 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    13261 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    13321 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    13381 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    13441 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    13501 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    13561 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    13621 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    13681 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    13741 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    13801 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    13861 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    13921 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    13981 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    14041 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    14101 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    14161 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    14221 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    14281 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    14341 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    14401 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    14461 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    14521 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    14581 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    14641 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    14701 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    14761 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    14821 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    14881 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    14941 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    15001 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    15061 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    15121 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    15181 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    15241 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    15301 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    15361 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    15421 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    15481 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    15541 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    15601 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    15661 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    15721 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    15781 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    15841 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    15901 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    15961 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    16021 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    16081 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    16141 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    16201 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    16261 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    16321 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    16381 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    16441 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    16501 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    16561 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    16621 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    16681 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    16741 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    16801 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    16861 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    16921 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    16981 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    17041 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    17101 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    17161 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    17221 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    17281 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    17341 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    17401 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    17461 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    17521 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    17581 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    17641 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    17701 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    17761 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    17821 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    17881 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    17941 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    18001 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    18061 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    18121 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    18181 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    18241 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    18301 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    18361 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    18421 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    18481 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    18541 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    18601 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    18661 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    18721 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    18781 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    18841 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    18901 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    18961 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    19021 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    19081 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    19141 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    19201 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    19261 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    19321 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    19381 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    19441 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    19501 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    19561 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    19621 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    19681 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    19741 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    19801 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    19861 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    19921 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    19981 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    20041 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    20101 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    20161 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    20221 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    20281 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    20341 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    20401 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    20461 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    20521 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    20581 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    20641 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    20701 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    20761 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    20821 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    20881 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    20941 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    21001 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    21061 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    21121 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    21181 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    21241 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    21301 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    21361 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    21421 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    21481 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    21541 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    21601 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    21661 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    21721 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    21781 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    21841 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    21901 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    21961 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    22021 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    22081 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    22141 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    22201 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    22261 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    22321 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    22381 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    22441 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    22501 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    22561 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    22621 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    22681 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    22741 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    22801 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    22861 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    22921 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    22981 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    23041 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    23101 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    23161 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    23221 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    23281 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    23341 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    23401 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    23461 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    23521 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    23581 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    23641 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    23701 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    23761 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    23821 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    23881 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    23941 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    24001 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    24061 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    24121 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    24181 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    24241 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    24301 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    24361 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    24421 aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta
    24481 tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa
    24541 tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg
    24601 ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat
    24661 gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc agccacacta
    24721 gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg
    24781 gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa
    24841 tcaacaggtc cgcaataaga aatggttcct ttcagactat cttttggaag tagctgtttg
    24901 attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt
    24961 tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct
    25021 ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat
    25081 tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact
    25141 aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt
    25201 acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta
    25261 ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct
    25321 tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag
    25381 aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga
    25441 atgattccga agaaccatcc gataaattta agaattttct ttttcataag gctccctcag
    25501 tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga
    25561 tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc
    25621 attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta
    25681 agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct
    25741 ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct
    25801 tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa
    25861 taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat
    25921 gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt
    25981 gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa
    26041 atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc
    26101 ttattggcaa atcgccaaaa taatgtagac taactaaaaa gactaagaaa caataacaaa
    26161 agttaacact tcttagtctt tctttagcac gtctatttat taaagatttt agggcctttt
    26221 ctcatgtttt tacgatcatt cttaggtgtt tccatttgtt tggatcggcc tccaccttgt
    26281 ttctttttga tcaattcttt cattttttct ttttggtcca tttttattct cctttaacga
    26341 atgaagcgac taattcgata tgatgggttt gcgggaaaag atctacaggc tgaactttct
    26401 tcacggcata acctgcttct gcaaataact tagcatctct tgcaaaagtt gccggattac
    26461 atgagatata gacgattcgt tctggtgaca tttcgattgc tgattcaata aaatgatgat
    26521 ctaatccttt tcgtggaggg tcaactacga tcacagatgg tttgatccct ttttcttgcc
    26581 attttttcat aactttttct gcttttccga cttcataatg aacattttcg attttattat
    26641 ttaaagcatt gaactgtgca tcttcaattg cttctggaat aacttccatc ccatacacct
    26701 tatccactct gtctgctaaa gacaagccaa tcgtacctat tcctgaataa gcgtcgatca
    26761 caatgtcttt tttatctaac tcggcaaact cgatggctgt ttggtacaga tactctgctt
    26821 gttgagtatt tacttgataa aatgactttg ctgagattcg ataggttttt cctaatagct
    26881 gatcttcgat atagtctctg ccatataaaa ctttttcttt gtctcctaaa atcacatttg
    26941 ttttttcttc attgatattt tggatgaccg aaaccacttc tggcaattct tcatgaatag
    27001 cttcagcaat ttccttggat ttaaagaatt tttctttacg agtaactaaa acgaccatca
    27061 tttcatggga ataatgacca cgacggataa tgatgtgacg aagctgtcct tcattttttt
    27121 cttcattata tcctcgtact tggaaccgtt gcaagatatc tctgactact ttgattgctt
    27181 gatcgatgac aggatcttgg atataaaaat cttcgatagg gactagatca tggctattct
    27241 ttttgtaaaa accggtggct aactgtccat cgatacggcg aacagggatc tgtgctttat
    27301 tacggtagcc gaatggttct gccattccga ccgttggaag tacaggaact tctggcatct
    27361 tagcaatctt cgccattaca tgctctactt gtttttgttt gaagacaagc tgttgttcat
    27421 acttcaaatg ggctaaaggt gcgataccta tgcgaagtaa tgcattgttt tctacaggct
    27481 gacgatcagg tgattgtgta ataaatgttt cgacttttcc atatccaaac ttattgcctg
    27541 cttttactac tagtatttct acttcttctc cagggagtgc attttcgata aataatggat
    27601 aaccatcgat ttttgctacc cccattcctt cataactcaa gtcgacgatt tcgactgtat
    27661 atcgttcgtt tttctttact atttgctgtt ctgtcatatt ttgctccttt aagcctattt
    27721 ttcaatccta ctgaactcta ctataaatca gctaattaca caactaaaac cggcactgac
    27781 cttactacgt tcagcaccgg cttttgatta attcttttga ttaggtgttt cgtctgctat
    27841 ttttccgtca ccgtcgatat cttcatccgt taaacgttcg acttctttta cgaattcttt
    27901 gctgacaacc tctaattctt cttcatctgt acctgtgatt gcttcatcag gaatctgatc
    27961 ggtattggca aagaactcaa tgtgttgatg aagattttgg aaatgcatcg gtgcttcccc
    28021 gccgtattca ccatctaagt tgatcattac tggtcgattg atatcatctt ctgtttcaac
    28081 gaacaaactg cttgttttgg tatagattat tcttggatca tcgacatgtc gaccgccatt
    28141 cagcatgagt gctgcaatat gcaagatctc aaagatattt gctgtcttca caatgattaa
    28201 agaaaacttc ccatcatcca atttggcatc tggcgcgatc tgttcgaatc ctccaacaga
    28261 gttcgtcagc cctaggaaaa acatcgaagc atttccgata tattcccctt catcatactt
    28321 caagcgcatt ttgattggct ttactcgagg gagcatctct gctcctttcg caagataagc
    28381 aagataacca aaaatactct ttagttctga tggaacttca taagtcaatt ctgtcaaata
    28441 gccacctgca gcgatattga tgaagtaact gttatcagaa aggccgatgt ccatttttac
    28501 ggtttggtgt ttttttatca cttctgctgc tgctttaata ttatctctag gaatttttaa
    28561 tgctcgagca tagtcgttcg ttgtaccagc tgggatgatg gccattttag gacggtgttc
    28621 aagcggagcg attccattaa ccacttcgtt gattgtgcca tcgccaccag ctgccactac
    28681 tagatcaaaa cctagttctg cgactctaca tgcttcattc ttggctgaat ctggttctgg
    28741 tgtagttgca taggcgctgg cttcaaatcc tgcttcttct aatgcctgta ggatatccgc
    28801 caaatttttt ttcaataatt cttttcccga ggtggggtta taaattacgc gtgctttttt
    28861 cattttcagc ctctatcgtt tcgctaattc atctttaagt agcttattca cgacaccagg
    28921 atttgcttgt cctttagttg ctttcatgat ttgaccgact aggaagccaa ccgcacgatc
    28981 ttttccattt ttgaaatcat cgacagattg ttggttgtta tccaaaattt cattaatgat
    29041 tggcagtagt ttgttcggat cagataattg cacccaacca tttttttcaa ctacttcttg
    29101 ggcttctccg ccatttgtga tcaattcacg gaagactttt ttggcgattt ttgagctgat
    29161 cgttccatcg gcgatcaaac gaatcattcc tgctaagttc tcaggtgtca attttgtctc
    29221 agctaattcc agtttctcac tgttcaagta agcagaaact tcacccatca accagttaga
    29281 tgcttgcttg gcatctgcac cattttccaa aacagcttca aaaaaatcag acatttcttt
    29341 tgtaagtgtc aataccattg cgtcatactc tggaagtcca aactcacgga tatagcgttc
    29401 gcgacgagaa gctggcatct caggcaaact agcttttact ttttgaatcc attcgtcatc
    29461 gatcacgaat ttcgggatat caggctctgg gaagtaacga taatcactag acccttcttt
    29521 gacacgcatc aaaactgttt tattcgttgt ttcgtcaaaa cggcgtgttt cttgttggat
    29581 ttctcctcct gaaagaagaa cttttgcttg acgtttttct tcaaaagcta aacctttttt
    29641 cacgaaagat aaagagttca agtttttcaa ttctgctttc gtaccgaact cttcttgtcc
    29701 ataagggcga agagaaatat tggcatcaca gcgcatagag ccttcttcca ttttcacatc
    29761 gctgacttct gtaaattgga taattgatcg caatgcttct agataagcgt atgcttcttc
    29821 tggtgaacgc atatcagctt cagaaacgat ttcgatcaaa ggcgtgcctt gtcggttcaa
    29881 atcaacgtaa gaataaccgc caataccatg catgtttttt ccagcatctt cttctaaatg
    29941 gacacgttcg atccggattt ttttcttttt accttctact tcgatttcga tccatccgtc
    30001 atgaccgatt ggttgatcaa attgggaaat ttgatatgct tttggattat ctgggtaaaa
    30061 gtaatttttc cggtcaaaat gagtatcttt agagatttca cagtttaatg ccaaagctgc
    30121 tttcatcccg aattccagcg cagctttatt cattactggc agcacgcctg gataacccca
    30181 gtcgatcacg tttgtattgc tgtttggttc tgcaccaaaa tgagctggcg ctggagagaa
    30241 aatttttgag ttggttttta actcgacgtg gacttctagt ccaatgactg tttcaaaatt
    30301 cattcgtctt tccctcctaa aatagctggt tgttgtttat ggaattctgt tgcttgttca
    30361 aacgcatatg ccgcttgata catcgttttt tcatcaaaat aattaccgat gatctgcaat
    30421 ccaactggta atccttctga taaacctgcc ggtacagaca tacctggaag tccagccaag
    30481 ttgacaggga tcgttaagat gtcactcatg tacattgtga ttggatcgtt aatgttctca
    30541 ccgattccga atgcaactgt tggtgaagat ggcccgatga tcaagtcata atctgcaaag
    30601 actttttcaa aatctcgttt gatcaatgta cggacttgtc ctgctttttt gaagtaagcg
    30661 tcataataac ccgcgcttaa ggagaatgta cctaacatga tacggcgttt tacttcttca
    30721 ccgaatcctt ctgaacgtga gttgacatat acatcatcta atgatttcac attttcagag
    30781 cgataaccgt agcggatacc atcgaatcgt tgcaaattgg aacttgcttc tgatgaggca
    30841 atgatataat aaacagccac accgtatttt gaatgaggca agctgacttc ttccacaaca
    30901 gcacctaatg aacggaatgt ttctgctgct ttcaatacag cttcttttac gccaggggcc
    30961 acaccttcgc ctaagtattc tttaggtaaa gcaatcttca ttcctttgat gtcgcctgtc
    31021 agtccctctg caaaatcagg aactgaaacg cctgaagaag taccgtcttt ttcgtcataa
    31081 ccgctgatag ctgtcaatgc taatgcatta tctttcaccg tacgggtcat tggtccgatt
    31141 tggtcaagag atgaagcaaa ggcaatcaaa ccaaaacgtg agacacggcc ataagtaggt
    31201 ttcatcccta cgatgccgtt aaaagctgct ggttggcgga tacttccccc agtatcacta
    31261 cctaaagaaa ctggaatttg tccagctgca actgctgctg cagaaccacc agaagagcca
    31321 ccaggtactt ttgttgggtc ccaagcattt tttgttttct tgaaataaga agtttctgta
    31381 cttccaccca tagcaaattc atccatgttt agcttaccaa cagggatcat atctgcttga
    31441 tagattttat ccatgaccgt tgcatcataa attggttcaa aattgtaaag gatcttagat
    31501 gcagcggttg tcaagatatc ttttgttacg atattatcct tgataccaat tggaatacca
    31561 gcaaggacgt tgctttcatc aatccctttt tcatctaaag cttttgcctg ttccaatgct
    31621 ttttcttcgt tcaatgtaat gaatgcctcg acctcaggct ctgtttcttt gattcgttca
    31681 aatgttgttt ttgtcaaatc ttgtacagtg atttctttcg atacaagcat atgatgcagt
    31741 tcttctagag aacgattgtg taattctgtc attatgcgcc agcctcccca ttatcgatga
    31801 ttgcgggtac tttgataaag ccgtcttctg tttccgtcac atttttcaat aattcatcgc
    31861 ggttccatcc agcttgcgcc cgatcttgtc gcatcacgtt tactgtttca accacattcg
    31921 acgtaaatgg aacaccttcc gtatctactt cttccagcat ttcaaccata tcgatgattt
    31981 tacctaattg gtcggtaaaa cttgcaagtt cttcatctgc gaacgcaagt tttgctaatt
    32041 tggcaacgtg tttcacttgt tcttcactaa tcgccatact accccttctt tctaaacgag
    32101 ttaaaaactt tgctctctat tttaacacaa gagtgccgtg tctgaaattg tttattgaaa
    32161 tttgtctgaa aaaactcatt tttcattctt ctactgagcg acttttagcc aatgcatctg
    32221 ccatttgatc ttctgtccaa acttcgattc ctaattcttg tgctttggtc agcttgctgc
    32281 cagcatcttc tcctgctacc acgatatccg tttttttcga tacgctacca gtcactttcc
    32341 cgccaagatt ttcgatcgtc tcttttgctt cttcacgagt aaatcgtgtt agtttaccag
    32401 taaggactac tgttttttct ttaaaaggag actcgacttc ttgcaattgg gcatttcgaa
    32461 gccctttgta ttcgaaattg acgcctgctt gttttaattc attcatcaat tcatggactt
    32521 cttcgttact gaaataagtg actacactgt cggcaatgat atcaccgatc gtatcaagcg
    32581 caataatcga ctcatagtca cttttactca atgtttctag atcaccgaaa tgttctgcta
    32641 aaatttttgc tgctttcgct ccgacatgtc ggattcctaa cccgaaaatc aaccgctcga
    32701 ccgaattatc tttgctgttg tcgattgctg tcaaaatatt atttgctgat ttttctttta
    32761 ttttgtccaa cgtcaataac tcttcttctg tcaatttgta aagatcagca acatctgcta
    32821 ctagtttttt atcatacatc tgttctagta cgcgcggccc caacccatcg atattcatcg
    32881 catttcttga aacaaagtgg ttcaaacctt ctttcatttg agcagggcat ttaggattga
    32941 tgcaccgtaa ggctacttct tcatccaaat ggacaagttc acttccacaa actggacaat
    33001 gtgtaggcaa ttggtattct tcgctgtctt taggtcgttt atctaacacg acttgcgaaa
    33061 cttctgggat gatgtctccc gctttataaa tcagaactgt atctttcaag cggatatcct
    33121 tcaacttaat ataatcaccg ttatgcaggc tggcacggct tactgtcgtt cctgctactc
    33181 ggactggtgt catgatagct gttggcgtta ctacccctgt tcgaccgact gtccattcga
    33241 tgttttcgat tagcgtttca acttcttctg gcggaaactt gtaagctgct gcccatctcg
    33301 gtgctttgat agtaaaacca agttgatctt gtagcgagaa ttcattcact ttgatgacga
    33361 ttccatcgat ttcatagggc aaatccacac gtttgtcatg atattcttca atgtatgacc
    33421 acacttcatc aatcgaatga catagacgtt tttcccgatt cgtgtggaat ccaatttttt
    33481 ctagctcttc tagcgcatca tactgggttt ttgcttgcat aggaccgaaa tcagctactg
    33541 tgtataaaaa agtgtccaga tttcttttcg ctgtgatttt agaatctaac tgacgcaaag
    33601 aaccagctgc ggcgttccga ggattcgcaa agatatcttt tccctctgct tctctgtctt
    33661 ggttcaattg gacaaatgac cgttttggca tgaaacattc gcctcttact tcgatgttca
    33721 ttggttcttt taatttgatt gggaccgacc ggacagtttt taggttttca gtgatgtttt
    33781 ctcctacagt tccatccccg cgagtcgcac ctctcacgaa atttccatct tcataacgca
    33841 aagaaacgga aagtccatct atttttagtt cacagcaata gtcaactaca cgtcccactg
    33901 ctttttgtac acgttgatca aaagctatca gctcttcttt gctgaaaacg tcgttcaaac
    33961 tgtaaagcgg aatatcatga gtgacttttt caaaaccttc taatactttt ccgcctactc
    34021 gttgagtggg agaatcagac gtgatcaaat caggatattg ttcctcgatt gctactaatt
    34081 ctgcgtattc tttatcataa acatagtctt ccactgttgg tttatcttct acgtagtatt
    34141 cgcgagacca ttggttcagt cttgtacgaa gttcttctgc tcgtttagca gcttcgtcaa
    34201 tcgtcatttc atgttccatc atttaccctc cactactaag ctttttcaat tggcgcaaag
    34261 gcagccaaaa gacgttttac tccttgactt gggaaagcca catctagttc tagatccttc
    34321 gacgtcccgc ttactcggac gactgttcct agaccccatt ttttgtgttt gactttatct
    34381 ccaggtttcc agctgttatt ttcccctcca gtagcacttt tagagggaac aaccgctgtc
    34441 tctggctgtt tatagctata tgctgttttt ttagcgaata caggcgtttt tttaggcgta
    34501 ggacgcatgc cttcgatttc cagtaactcc tgatcgattt cttctacaaa acgagaaggt
    34561 cggttatatt gcgtccgtcc atataatgtt ctggagaaag catttgttaa atacaacgct
    34621 tcttctgctc gcgtgatccc cacatatgcg agacggcgtt cttcctccag ttcgctttct
    34681 tccatcaatg ctcgagataa aggaaaaacc ccctcttcta gaccaatcag gaatacaact
    34741 ggaaattcta atcctttggc tgcatgcaaa gtcatcaatg tcacttggga agcatcttct
    34801 tctaaattgt caatgtcgga aacaagtgcc agatcattta agaagaccgt tagtttttct
    34861 tctggtgcat cagcgtcttc ttcattttgc tgttcgaatt gtttgtcgaa ttcttgtgtg
    34921 accgttaaaa attcctccaa gttttcgatg cgtgcttgtg cttctaatgt attttggatt
    34981 tttaagtctt ctagatatcc gctacgatcc aaaacctctt tcgtcaattc tgtcactgtc
    35041 aagtatggga tcatctgagt gacttcttgg atcatctctc caaatgcacc taattgctgt
    35101 cctgcttttc caccgatatt tgccagatcc acattttgcg ctgcttcaag cagtggccat
    35161 tcatgaagag aagcaaaact gcgtagtttt tcgatagatc ctggtccgat gcctcgtttg
    35221 ggcgaattaa cgatacgctc aaaactgatt gaatcttgag gatttaccag cacattcaaa
    35281 tacgctaaaa tatctttgat ttctttacga tcgtagaact tgtgccctcc aaccatttta
    35341 taaggaatat ttgctttcaa cagagtttct tccattacac gagattgtgc gttcgtccgg
    35401 tacaagatgg caaagtcgcc gtaattccga tgattgcttc gaatctcttc ttgcatccgg
    35461 tctacgataa aacgggtttc gtctcgttca ttatctgcac ggtagtaagt gatcttgttt
    35521 ccttctttgt tttctgtcca caagtttttt ggttttcgat tgctattgtt ttcaatgact
    35581 tggtttgctg cgctaaggat gtttttcgtg gagcgatagt tttgttctag caaaattact
    35641 gcagcatctg gataatcttt ttccaaatcc aagatgttct gcatatctgc accgcgccag
    35701 ccgtaaatac tttgatctgc atctcccacg acgcacaggt tcctaaatct acctgccaat
    35761 aaattgacta gcgtatactg tgcgtgattt gtatcttggt actcatctac atgaatataa
    35821 tggaatttgt tttgataata cgtgagtgaa tcaggatgtt cttcaaataa tcgaatcgta
    35881 ttcatgatca ggtcgtcaaa gtccatacat tgattatatt gaagctcttt ttggtaagca
    35941 gcataacatt ttgccgcaat ctcttcgtaa taactgcctt gcatttttgc aaaatcctga
    36001 ggggtctgca ggctgttttt tgcttgagag attgttccta atatacttcg aggatcatat
    36061 tttttcggat caatgttcag ttcttttagt atccgtttca tcaaggtcag ctgttcagaa
    36121 gaatcgatga ttgtaaagtt ccggttatac ccgataaaat caacatctct tcttaaaata
    36181 cgcacacaca tcgagtgaaa agtcgatacc cagacatctt cgcctccaga tgcgagaatc
    36241 gcattgacac gttctttcat ttcttttgca gccttattgg taaaagtaat cgctaggatg
    36301 ttccaaggat tgacttcttt ttcctcaatc aaataggcaa tgcggtgtgt cagcactctc
    36361 gtctttccac ttcctgcacc tgccattagc agtaatggtc cgtcagtgtg aagaacagct
    36421 tctttctgac gggggttcat cccgtttaat aattcagctt tggatgacat taacaacatc
    36481 ctttctttta tcaatcttgt taattatagc attttcttgg ctttcattaa actagctagt
    36541 tcaaaatcat ttgtttcttt ttatggattt ttccttgtca attcgttgat ttcgcgctaa
    36601 attaaaagaa agcgaggcgt ttgaatggaa aaaaaaggaa aaatcaagaa attagataag
    36661 actcagatat attttattct aaaaggaata tttgttggac tgctgacagg aatcgtcgtt
    36721 agcctgtttc gattatctat cgaaaagcta agcgatttag ttcgaagtat ctatgaaatg
    36781 agtcgggaac agccgattta cttaattggc ctagctattt gctgcatcct tgcagcattt
    36841 ttcgtgggtt atctggtcaa aaatgaaccg gatatcaagg gaagcgggat tccacaagta
    36901 gagggacagc ttcgcgggga attatcaatg aactggttct ctgttttatg gaaaaagttc
    36961 attggcggtg tactttcagt tggtgcgggc ctttttctag gtagagaagg accttctata
    37021 cagcttggcg catccattgg acaaggagct ggacaattgt ttcggagtcc atcttcagaa
    37081 gaaaagattt taatttctag cggtgcaagt agcggacttg cagcagcatt caatgctcct
    37141 atcgcaggat tactctttgt tttagaagaa atccatcact ccttttctcc acttgtttgg
    37201 ctgacatcat ttgcttcagc aatcactgct aacttcgtct ctctttactt tttcggcttg
    37261 agacctgttt tatatctagg tgaccttcct tctttgccgt tgaaatatta tggttcctta
    37321 gtgatcttag gagtacttct aggcatctta ggttttattt atcagaatgt cttgctggct
    37381 ttgccaaaat tttacaaccg attgccttta ccagcttatt ttcacgggtt cgttcctttc
    37441 ttgctgattt tgccaattgg ttatttatgg cctagtactc taggcggagg caatcaaatc
    37501 gttttagctt ttggacatgc ttccttgcca atcattatga ttatcggttt gtttgttctt
    37561 cgattcgttt tttccatgat ttcttacggt gggggtcttc caggggggat tttccttccg
    37621 atattgacat tgggggcttt aatcggtagc ttttatggaa acattatggt tgatttcggg
    37681 atggatccaa tctacatcaa ggattttgtc gttattgcca tggctggtta tttcacagcg
    37741 attggaaaag caccgctgac agcaattatt ttagtgacag aaatggttgg ctctcttaat
    37801 catctgatgc ctttaggatt ggctgctctt gttgcttata tcgtcaatga ccttctcggc
    37861 ggtaatccaa tttacgaatc attattggaa cgtctacttg gaaatactca gaccgcctca
    37921 ctaaaaggtg caaggacaac ttttgatttt ccagtaacgg cagagagttc tttagacgga
    37981 ttaatggtac gagacttcaa ttggcctaaa aatatgctgc taatctctat acgcagagga
    38041 aatcaggaaa tcttgactca cggtgatacg atcatgaatg taggagatat tttggttatc
    38101 ttgactgatg aagcaaactt gccaagaatc aagcaggaaa tcgaggctaa atcttctatc
    38161 acaaatttga agcaatcatt ttaacgaaca tggaaaaact attttacaac aactattcat
    38221 ctacactact tgattctata tgattctata aaaaaagaag aggctgggaa aacccagcct
    38281 cttctttttt atttctatgc ttctgctact gcaggagctt ttctcaataa accataaatc
    38341 acaccagata cgactgctcc gatagcaata aacaccaaat acatgaccgg gtgactcagt
    38401 aagaatacaa cgaaaatccc gccgtgagga gccagtaatt tgattccgaa agctccgact
    38461 aatccgccag ttaaagcaga accaataata aaacttggaa tggcacgaat cgggtcagct
    38521 gctgcaaatg gaatcgatcc ttccgtgaca aacgatagtc ccatcacgat gttcgtcaaa
    38581 ccagcatctt gatctgtttt gctgaattta ttcttaaaca aacgtgtagc aacgaaaatt
    38641 gccaatggag gtaccattcc tccagccata acagaagcca tcacgacact tccgccagtt
    38701 gcaacagtcg tagctaaagt tgctgtacca aacacataag cggctttgtt gattggaccg
    38761 cctaaatctg ccgccatcat tccagctaat aatgcaccaa gtaaagcagc attcgaacca
    38821 ctcatacttg tcaagaaacc gttcaatgca tcattgattg ctttcatagg gacattgacc
    38881 agcaacatca agaaaccagt aatcagtaag ccaaacactg gataaaagag gatcgtttta
    38941 attccatcca atgattttgg taagttcttg aataatttac gtaaaaatac gatgacataa
    39001 ccagctaaga atccaccaat cagtgcacct aagaatcctg caccgcctgt attagctaat
    39061 gcaccagcag caaatccaac gatcaaacca gggcggtcac cgatacttga agcaataaat
    39121 cctgctaata ccggcagcat gaaaccaaat gcagcttgtc cgatttggtt gaaccagcta
    39181 gcagcttgat tgtaattccc tagatttgat aattgatcat gtggcacgcc aataaattgg
    39241 tcgatcatga aagaaagcgc aatagcgatt cctccaccaa taacgaaagg taacatgtgt
    39301 gatacgccgt tcatcaaatc tttgtaaatt ctttgtccga tagtaccatc cgcatttcct
    39361 tcttctgaac tgctttcttt tccatcacca tggaatactg gtgcagtacc actaagggct
    39421 agattgatca actcttctgg tttacggata ccatcactga caggacgatt gatcagttct
    39481 tttccgtcaa agcgattcat ctctactttt ttgtctgctg caacgattac accttctgca
    39541 cgggcgatgt cttcagcagt caatcggttt ttgattcctt ctgaaccgtt cgtttcgact
    39601 ttgatctcca cacccatttc tttcgctttt ttcttcaaag catcttctgc catgtaagtg
    39661 tgagcgatcc ctgttggaca agctgtaacg gcaacaacga attttctttc tggatgagca
    39721 gaagcttctg cagcttgtgt ttcttgttct gctttttctt ctgcttcttt ttgctcttca
    39781 gctgtttgga acaagtcttg gacttcttct ggtgtatttg cttcttttaa ttttcctacg
    39841 aaatctgcat caatcagcaa tcgagatagt gctgctaatg cttgcaaatg tgtgtcgttt
    39901 gcgccttctg gagctgcgat catgaaaaac agataagttg gctgtccgtc taaggcttca
    39961 taatctactc ctttttggct ttttgcgaat aaaactgttg cttctttaac tgcactgttt
    40021 ttcgcgtgag gcatggcgat tccatcacca agacctgtag aagtttgtgc ttcgcgggct
    40081 agaattcctt ctttatatgt ttcgatatcg gaaatccggc cggcatcaaa cattttttgg
    40141 accatttcat cgattgcgcc ttttttatca gtcgcttgca aatccatgat catcgcttct
    40201 ttgaccatta aatccttgat attcatgatt atcctccttg aaactaatca atttttgtga
    40261 tttcaacttc tggcagcaac tcatcaatca attcgcgagc tgccaagtca tcagaaaaag
    40321 ctgttgcgct tccgcaagca actccccatt taaaggcttc gattggatct ttcgtacggc
    40381 tgtaagcccc aataaagcca gcaatcattg aatcccctgc acctactgaa tttttcaatg
    40441 ggcgaactaa tacattcgtt ttataagttc cttgttccgt gaagagcaaa gcaccgtctc
    40501 cagccattga gatcagcaca ttctgtgccc cttcagctag taattttttc ccatacggaa
    40561 tgatttcttc tacagattgg aaggttgtgt tgtaaagctc agctaattcg tgattgtttg
    40621 gtttgatcaa caacggtttc ttaacaagtg cattttttag gtcatcacct gttgtatcaa
    40681 tcacgaaatc tgcacctttt tctcgaatga tatcaatcag ttcttgataa aatccttttc
    40741 gcaggctagc tggcgcgctt cctgacaata cgacgatatc tccttgtcct actgttccta
    40801 atgcttgctt caactcatcg atttcttcat ctgaaataga tgggcctaag ccattgatct
    40861 cagtttcagt gtctgatttt agtttgatat tgattcttgt atcttctttt acggttgtga
    40921 acaaagactg gatttgttct ttttcaagcc acttcgaaat aaattcgcca gtgaaccctc
    40981 ctaaaaaccc tagtgctgta gattgagaat cgatccgttt caaaatacgg gaaacattga
    41041 tcccttttcc tccaggtaac ttgaagtctt ccttcattcg gttcaaatcg cctagtttta
    41101 acccatctac gcgtacgata taatcaattg atggattaag tgttactgtg taaatcattg
    41161 tattgcctcc ttaattgttg tcttttcacg gaattcatta acgaattcta atggacaacg
    41221 ctcggttatg atcgtggctg cttccaaagg tgcaacagag gtgaaggaca actggttgaa
    41281 tttggtatga tcaatcaata caaatgtctc ttcggaattt ttcatggcta aacgtttaac
    41341 agctgcctct tctggatccg gagtcgtgaa tccttgttca agatgggcac cgttcattcc
    41401 cataaatgct ttattaaaat gaaaattttt tagttgttct atcgcattag atcctaaaac
    41461 agcattagtt gccagcttta tcgttccacc taaaataatg gtagcaagct ccaaatcaac
    41521 taaatttgcc gcatgttgaa cggaatttgt cactactgtg atctgttttc cttgcagaaa
    41581 aggaatcatt tctaacgtag tagagccagc gtctaaataa atgacatctt cgtcattaat
    41641 ggtttcagct gccgatttgg cgatttgctg tttttcgtga acgtttttga gtgatttttc
    41701 actcatattc tgctcaaaat tcaataattg ctctttctta gctccgccat gaactctttc
    41761 tagaaaccca ctattctcca gctcttgtaa atcacgtctt atcgttgatt cagaagcatt
    41821 caaaaggttt acaagatctt gtgatttcac gattttctgc tgttcaagca agtctaggat
    41881 tttttgtctg cgttcctctg taagcatttt cacgatcacc tcaatacaaa taatagcata
    41941 ttttttttct actagcaata taaatcagtc aaaaacgttc ataaaaatta taaatcagtc
    42001 aaaaaaaaaa taaaaaacca gtcagaaaga ctggtttttt atttttttgt ctatcatcaa
    42061 gccgttttat cgttttttat tttcgcttct tgtttttttc atataaacag aagcaggttt
    42121 tctttcctta tgaagaagat aataataaag tcccatcccg ccaaacatga ccaaaaaacc
    42181 gatgactgat cgaataggaa aattaggaac aaaaaagaga cagacggata ataatgatgc
    42241 cggaaccatt attcgataat agagttgttt atccattttc ttcacctcat attttttgta
    42301 aagtaatcaa ttgttctaat tctcggtaat ctaaaaattg cacacgcaga tgctgctcac
    42361 ctttcttgac atcgaattgg atacttcgcc gctttttctt tattcgtttg aaggtaacat
    42421 cagagagtct atgtttgatt tcctttaacg gtaattctga cagggattct gaaggaacat
    42481 agattcttaa ggataaaaga tactcttgat atttttcatc tgttacctct acttctagat
    42541 attccctaat ataataacaa taagcttcga ttccattatc caaagcaaca gtcggatcga
    42601 taactttata gcacatcatg cgtgatcgat tctcgtcaac ccaaggaaaa tacgcctgag
    42661 catttagtgt tccgcgaaca cgcctttctt tataatctcc ttgttggata tcataacttt
    42721 ttgttacaaa aaatgaagca ttctttttag gaacgggttg catttgtgca ttctgatact
    42781 ggacagattg gatcgtatgg tcagtgtaaa tgtattcaac gaatggcggt ttcaataaac
    42841 cgacaccatt gtcccaagca agatgatcaa acccaagctg gtccctaaaa taggattgaa
    42901 acatttgatt gtttggcttt tgccagttaa aggggtctcg ataaagctgg atacatatga
    42961 cttcgttttc taattccttc actttttcta cttgttggat gtcgagaaaa atcggtttag
    43021 ggatgatttg atgataatag cgactataat acatttccca acacgaagtg aaacataaac
    43081 cttggtaaaa aagatcgtat cctggaaatt ggttgcaatc gactactact tccccgtttt
    43141 gttgtttcat tttaggtaaa tgttcgattt catccttttt ctcaaacaat agccgttgag
    43201 agatctgttt tgtattgtga tagataaact cgctataagc tcgcatatag gcgaacaccc
    43261 catgagcaat catttttttc tgaatgaagt ctcgaatcaa aaattgcttt tctttgaaaa
    43321 cagagataga caatacattt tttatcaata attgattatc ttccaagcga aaactacatt
    43381 cgttctcgtc atttcctaca actaaccaac tttcgtctct taccgatgct agcattattt
    43441 tttcttgaag ctttcgaaga tcatacggat ccgcttgttg tgtgaaagag cggataaatg
    43501 ttggctggat caacagacgt gtcaaaatag cccaatcgct tatttcctgt tttttttctt
    43561 cttcatttaa gtctgtgagc tggatagtca tcaataattt atcttcgcca atcattttct
    43621 cacctctttc aaatcttttt ataatttaat tataaccaat gataaagaaa aactaaaact
    43681 ttatgtctta gaaaccaatt tggacgatta catgatagac taagtaaaaa gaaacaaaga
    43741 ggtgtttaca atggggttga aatttgaacg tacagataat ttagataaac ttttcgaatc
    43801 atttgctgtc gatccggaaa aaacagttgc accaaaagaa gagatcgatc gatttctaaa
    43861 accacagcca caaaatgaaa agaaaaaaga caagaaacaa aagtaagtta agctcaaaaa
    43921 a
//
LOCUS       DACEUF010000012        43691 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.013, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000012 DACEUF010000000
VERSION     DACEUF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.013"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(117..641)
                     /locus_tag="IXG20_000665"
     CDS             complement(117..641)
                     /locus_tag="IXG20_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1608881.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(634..1107)
                     /gene="tsaE"
                     /locus_tag="IXG20_000666"
     CDS             complement(634..1107)
                     /gene="tsaE"
                     /locus_tag="IXG20_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ1608882.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1230..2210)
                     /gene="pta"
                     /locus_tag="IXG20_000667"
     CDS             complement(1230..2210)
                     /gene="pta"
                     /locus_tag="IXG20_000667"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ1608883.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2363..3043)
                     /locus_tag="IXG20_000668"
     CDS             complement(2363..3043)
                     /locus_tag="IXG20_000668"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1608884.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3169..4038
                     /locus_tag="IXG20_000669"
     CDS             3169..4038
                     /locus_tag="IXG20_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1608885.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4064..4579
                     /locus_tag="IXG20_000670"
     CDS             4064..4579
                     /locus_tag="IXG20_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608886.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4626..5189)
                     /locus_tag="IXG20_000671"
     CDS             complement(4626..5189)
                     /locus_tag="IXG20_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1608887.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5244..5423)
                     /locus_tag="IXG20_000672"
     CDS             complement(5244..5423)
                     /locus_tag="IXG20_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608888.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5500..6009)
                     /locus_tag="IXG20_000673"
     CDS             complement(5500..6009)
                     /locus_tag="IXG20_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608889.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6105..6485)
                     /locus_tag="IXG20_000674"
     CDS             complement(6105..6485)
                     /locus_tag="IXG20_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1608890.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6815..7564
                     /locus_tag="IXG20_000675"
     CDS             6815..7564
                     /locus_tag="IXG20_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ1608891.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7748..9025)
                     /locus_tag="IXG20_000676"
     CDS             complement(7748..9025)
                     /locus_tag="IXG20_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608892.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9104..9748)
                     /gene="pcp"
                     /locus_tag="IXG20_000677"
     CDS             complement(9104..9748)
                     /gene="pcp"
                     /locus_tag="IXG20_000677"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1608893.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9947..10891)
                     /locus_tag="IXG20_000678"
     CDS             complement(9947..10891)
                     /locus_tag="IXG20_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1608894.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10893..11579)
                     /locus_tag="IXG20_000679"
     CDS             complement(10893..11579)
                     /locus_tag="IXG20_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1608895.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12053..12373
                     /locus_tag="IXG20_000680"
     CDS             12053..12373
                     /locus_tag="IXG20_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1608896.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12527..13432
                     /locus_tag="IXG20_000681"
     CDS             12527..13432
                     /locus_tag="IXG20_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1608897.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSYKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSIKGEEEK"
     gene            13429..14202
                     /locus_tag="IXG20_000682"
     CDS             13429..14202
                     /locus_tag="IXG20_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1608898.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14383..15807)
                     /locus_tag="IXG20_000683"
     CDS             complement(14383..15807)
                     /locus_tag="IXG20_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1608899.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16018..16464
                     /gene="nrdI"
                     /locus_tag="IXG20_000684"
     CDS             16018..16464
                     /gene="nrdI"
                     /locus_tag="IXG20_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1608900.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16529..18511)
                     /locus_tag="IXG20_000685"
     CDS             complement(16529..18511)
                     /locus_tag="IXG20_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1608901.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18525..19421)
                     /gene="murQ"
                     /locus_tag="IXG20_000686"
     CDS             complement(18525..19421)
                     /gene="murQ"
                     /locus_tag="IXG20_000686"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1608902.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19424..20485)
                     /locus_tag="IXG20_000687"
     CDS             complement(19424..20485)
                     /locus_tag="IXG20_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1608903.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20696..21223)
                     /locus_tag="IXG20_000688"
     CDS             complement(20696..21223)
                     /locus_tag="IXG20_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1608904.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21290..22792)
                     /locus_tag="IXG20_000689"
     CDS             complement(21290..22792)
                     /locus_tag="IXG20_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1608905.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23050..24171)
                     /locus_tag="IXG20_000690"
     CDS             complement(23050..24171)
                     /locus_tag="IXG20_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1608906.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24297..25238
                     /locus_tag="IXG20_000691"
     CDS             24297..25238
                     /locus_tag="IXG20_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1608907.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25388..26284)
                     /locus_tag="IXG20_000692"
     CDS             complement(25388..26284)
                     /locus_tag="IXG20_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1608908.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26553..27893
                     /locus_tag="IXG20_000693"
     CDS             26553..27893
                     /locus_tag="IXG20_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1608909.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28111..28554
                     /locus_tag="IXG20_000694"
     CDS             28111..28554
                     /locus_tag="IXG20_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1608910.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29058..30476)
                     /gene="nhaC"
                     /locus_tag="IXG20_000695"
     CDS             complement(29058..30476)
                     /gene="nhaC"
                     /locus_tag="IXG20_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1608911.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30879..32102)
                     /locus_tag="IXG20_000696"
     CDS             complement(30879..32102)
                     /locus_tag="IXG20_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ1608912.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32133..32972)
                     /locus_tag="IXG20_000697"
     CDS             complement(32133..32972)
                     /locus_tag="IXG20_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ1608913.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32989..33669)
                     /locus_tag="IXG20_000698"
     CDS             complement(32989..33669)
                     /locus_tag="IXG20_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1608914.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33671..34744)
                     /locus_tag="IXG20_000699"
     CDS             complement(33671..34744)
                     /locus_tag="IXG20_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1608915.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(35391..36062)
                     /locus_tag="IXG20_000700"
     CDS             complement(35391..36062)
                     /locus_tag="IXG20_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1608916.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36149..38827)
                     /gene="mgtA"
                     /locus_tag="IXG20_000701"
     CDS             complement(36149..38827)
                     /gene="mgtA"
                     /locus_tag="IXG20_000701"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ1608917.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39486..39923
                     /gene="ndk"
                     /locus_tag="IXG20_000702"
     CDS             39486..39923
                     /gene="ndk"
                     /locus_tag="IXG20_000702"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ1608918.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39945..40811)
                     /locus_tag="IXG20_000703"
     CDS             complement(39945..40811)
                     /locus_tag="IXG20_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1608919.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40971..43064)
                     /locus_tag="IXG20_000704"
     CDS             complement(40971..43064)
                     /locus_tag="IXG20_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1608920.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        12616 a         9153 c         7718 g        14204 t
ORIGIN      
        1 ttgaacaccg tttattcaaa aaaaatggac tgcgacacga cttttgtcac agtcccttcg
       61 tgtgtttttc tgccttctca aaaggatgac agtttctcac tcttttccta tttcttctat
      121 tcaatcaaat aactcatttt atatacatct aaaaattcac ctagatctgt tcttgcccct
      181 ctaggcatga ccgcttcgat ttggaagccc atttttcggt aaaggtgtac ggctctttca
      241 ttacgaactt gaacgtcaag ctccaatcga aaaagtacac ccatttcctt cgcccaactg
      301 atgatttctt ctagcataag agtgcctagt cccattcccc aatattcctg cagaatactg
      361 ataccgactt ctcccacgtg agacaaacga aattgactgt ctgccttgac agatgctgtt
      421 ccaataatcg tcccttcata gattgcaagc agcaataaat tattattgct ttcgtatata
      481 taatccaact cttcttctaa ggtttctggt ggtaacagga gttctgcttc atccaaaact
      541 aaaaattctg tctccttatt aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct
      601 ggcactgctt cccgcaagat gatcccaact tcttcactca tgattttcag cccatttttc
      661 tagaattcgc tgtttgaagg tttcactttg ctccccgtat gagattagtt tcacataccg
      721 cttttctaaa tctgtatcac ttttttctaa aatcagttcc aaataatcct ccggcagtgc
      781 ttcttctaat agatttcccc attcaacaac ggaaagccca tcaccttcaa aatattcatc
      841 taaccctaaa tctgctccgc tagctgctac ccgataaata tccatatgat acaacggaat
      901 ccgaccttga ctatattctc taataatcgt gtatgtcgga cttttgatca tttgcgttat
      961 ccctaatcct tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt
     1021 caatatcaga ttgtcacctg gcattgcgga atgaccaatt gcctctgcga attgttctgt
     1081 cattgttaat cctgatagtt cgatcatttt tcatcttctc ccgctcttct ttctttttgc
     1141 cagtatatcg cgtaatgaag tttttttaaa gaaaaaatga actagtcaag aacagcagct
     1201 gctgtttttg gctagttcgc gtgtatttgt taatcaatca atgtttgtgc tgctgtgata
     1261 atcgataatt tatatacatc ttcttcattt gctccacgag acaaatcaga aaccggtttg
     1321 ttcaagcctt gtagaattgg tccgattgct tcaaagttac cgaaacgctg agcaatttta
     1381 tagccgatat ttcctgactg caattctgga aatacaaaga ctgttgcttg accagctact
     1441 tctgaatctg gcgctttcag ttgtgcaact gaaggaacat aagaagcatc aaattgcaat
     1501 tcgccgtcga tcatgtattg tggcgctagt tttttagcga ttttagttgc ttcaactact
     1561 ttgtctactt ctggcgattt cgctgagcct tttgtcgaga agctcatcat tgctactttt
     1621 ggttcgatat cgaacaattc tgctgttttt gcagaatcta cggcgatttc agccaattct
     1681 tgcgcagttg gattcacgtt gatcgcacaa tccgaaaaga catatttttc ttgatctctt
     1741 ccacgtacca tgatcatcgc tccgcttgtc cgactgatgc caggctttgt tttgatgatc
     1801 tgaagtgctg ggcgaacagt gtcgccagtc gaatggatcg ctccagagac cattccgtct
     1861 gcaatcccca tatacgtaag catcgtacca aagtaattca catcttttaa aatcttttct
     1921 gcttgttctt tggatgcctt tccatttcta cgttcaacaa aagtatccac catttcatcc
     1981 cacttatcgt aattatcagg gctgatgatc gtaaagctag atgttttgat tcctctggca
     2041 tgcgctgcat ttcttatttc ttcttcgttc cctactaaaa tgggttccac aagttcttct
     2101 gctttcaacc gggcagctgc gcctaaaata cgagcatcag tagcttccgg gaaaacgatt
     2161 ttgatcccac ggcgaacaat tttaaatctt aaactatcaa acaattccac aagatgacct
     2221 ccagacgttt ttatatgtat tatcatacac tattccagaa aaaaagaaca gtttatttta
     2281 tctgtatcag tcaaaaatgc tggaaaaatt aagtttttca catacaaagc gaactgcact
     2341 ataacaggaa agcgctgttt tattacactg tttcaggcag ttgccaatca atcggttctt
     2401 ctcctaacgc aaggagcgca tcattcgttt tcgagaaggg tttggaacca aaaaagccac
     2461 gacttgcaga taacggactg ggatgtggag atgttatgat gatatggcga ctcttatcga
     2521 tcattttgat tttttcttgt gccggttttc cccacaagat aaacacgatt ggtttttccc
     2581 gttcatttaa tttttcaatg atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag
     2641 aatacgcttg cccttcccga acggtaagaa cggtgttgag taaaaggact ccctgtttag
     2701 cccatgacac tagatttcca tgttgaacag gtgcgattcc aagatcatca taaagctctt
     2761 tatagatatt tcttaacgat ggcgggatct ttacgccagg ttgcacagaa aaagataagc
     2821 catgagcttg attttctcca tgataaggat cttgtcctaa aatcaccact ttaacttctt
     2881 cataaggagt caattccaat gcttcataga tatgatacat atctggatgg atcttttggg
     2941 acgcatattc tttttttaaa aattctctta gatgcagata ataatctttt gaaaattctg
     3001 aggacaaaac ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc acctcctaat
     3061 aaaaagaata actcacattt tcttcaatca caagggacaa tcttcaccct cagcacttga
     3121 ttaatatggt aaactaggaa caagaagcag aaaggaacga taatcattat gatcaaatta
     3181 attgcatcag atatggacgg cacattatta gatgcccata tgagtgtttc tcctgaaaat
     3241 gccgaggcga tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga
     3301 aactatcaag aagctcgcgc agctttagat gaggtaggaa tcgattgtgc gatgatcact
     3361 ttgaacgggg cacaggtatt tgataaagaa ggaaattcat tgtttaccgt gtcgattcca
     3421 aataatcaag caatctctgt tttagatatt ctagatgcta acggtattta ctatgaagta
     3481 gcaaccaacg atggtcttta ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc
     3541 atggtggcta cccatttacc tcaccttacg tataaaatgg cgattgccat ggcctcagct
     3601 aagctggagt tattgcacat cacatacgtg gacagtattc gtgctatttt ggaagaaaag
     3661 aatctggaag ttttaaaaat tatttgtttt catacagaag ggccagctgt attaggtcca
     3721 gttggaaagc agatttctct tttaggcgat ctagccgtta cttcctctgg tcaaaacaat
     3781 atcgaagtga accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag
     3841 cgtgggattt ctttagaaga agtcatgacg attggcgata acttcaatga tgtcagcatg
     3901 ctgcaagtag caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat
     3961 gccaaatata caacggacac aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg
     4021 attgcagaag acttgtagtt ttatctagaa aagagggata tagatgtcag aatttttcat
     4081 tcaggaaaag cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc
     4141 cctgtttttg atggttggtc gctggggaac aaagggcgat gcattgtctc tttatgccat
     4201 gaatggcgat ctcgttgctc acatcaagca gataagcctg acattcggta cgcgttttga
     4261 gctgtataaa gagttcgaaa aggtgggaac catgcagaaa atattcaatt ggcccggtga
     4321 cttttattat attcgtagac ttcactggac agtccaaggt gatatctata atcatcaata
     4381 tcaaatccat catttcaatc agcaaatcat gaaaatggat aaagcaacac ttttttcagg
     4441 agattactat gtattggatg tctcaaatga tgaagatgct ccagtttgta tttgtattgc
     4501 tgcggtttta gattactggc tctaccgtaa agacaaaagt cgcgataagc ctgtttctta
     4561 ccgacttaac ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca
     4621 tctttttaga tattcttttg catatgatca taaagatgac cactgatcat ccgctgaccg
     4681 acaactttga agtcatggcg ttcgtataat tttttagcct ttggattttt ttcatccaca
     4741 cttagaccga tgacggagcg attagctttc ttcgccaatt taggtaatgc ttctaataat
     4801 cgcgaaccga tgccttgtcc tctaaaatct tctgatacac tgatagagtc caaataccat
     4861 tcgttgggaa acgtttcttt atcaatgaac aaacgtgtag acgcatctag ctgatatttc
     4921 tctaagatag gtaccagtgg ctgatcgatg attgattctt cttcatctgt ataaccgtaa
     4981 gcaactcctg caactttacc gtctatttct tctacgatac ctcgtgaata actgtaacga
     5041 taatttggat cagtgattgc ttcttctaaa atctctaaag tcttttgttc ccctgtctgc
     5101 ttcaaaaatg gcagttccat atcttttaga atcactaaga tcaatggggc gatttctgga
     5161 gcatcttctt ttctcgcaaa acgaatcata gtttcctctc cttttctcca ttctaacatg
     5221 cagaaaaaga aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc agtaatgaaa
     5281 taaccatgag taacgattct tgccgcttca gttcggcaat aagggcagac gactggcatt
     5341 tctttatcat tatgttcgac tacattattg acttcttcgt atgcaaattt ttttccgcaa
     5401 ttagggcatt tcattttttc catgttatct caacttcctt tcatctttac tctcttagct
     5461 tactcttata ttgtaaaaaa gagaagcaat atccttcttc tatgcagata tccatacctg
     5521 acggtcctgt tctccatcta ttaatcggac tttataataa ggacgttcat ctttgactag
     5581 aagtgtccac tctttcactt ttgctttttt cccgtagtag ctgactgctt ccttcatcgc
     5641 tgcttgcggt tgtttgaact cttccgtttt ttctatatca aaaagatgtt ctgcggcctt
     5701 ttccgtgtca tttcgtacgg ttgttttccc agtggatgga ttgactgcta tgacttgtcc
     5761 ttcacaaaaa aactggtatt catatgttgc attcgtagga ttctccaaat ctttttcaac
     5821 tgcaaaatga atagcagata tgttatgttc ttttgtttcg ttttttacga ttgctgctgc
     5881 ttttttcaat gagatccgat attcctggtc actaatatca tccatgtgaa aagcggcatt
     5941 gagcagtccc gtcataccgg taatccctat tacgatgagg gcagcaacac caaacatcat
     6001 ttttttcatt cttcattctc ctgtttcttt tttttaaaga atatcaagaa atgaacgaat
     6061 gaaaaatcga atgaaacgaa aaaaaagtga caattttgtt atctttattc gtgcagttct
     6121 aatggcaatc cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct
     6181 ctaatcggtt ctgattcgat ccctaaggac tgcaattctg caattgtttc ttcaatatcg
     6241 tctactttga aagcaagatg acgcaagcca caagcttctg gatagtttgg tcgttgtggc
     6301 gcattagcaa ttccaaaaat ctccaattca gcacttccta gtttcagatc taatttatag
     6361 tcatctcgtt ctttgcggta attttctctg attacttcaa atcctaattt attaacataa
     6421 aaatcacgtg atttctgata atctgaaaca atgatggcta tatgatggat cgttgtaagg
     6481 ttcatatgag cactcctatt cttcgtttaa gctcggatct atattaatct ccagttccat
     6541 tgtaaatgtg tcagattgaa aaaaatagct gtttttcctt tttataagaa aaaaggcaaa
     6601 aaaaacagaa agtaggtcct ccaagacact cttcctgttc ccaatataat aaatgaaagt
     6661 aagaaattat attttcaaga aaacaaacag aaaattatca taacacaaat tatttataat
     6721 tgaaagacta gttttttttc aagaaaagca tacaatagat tgtgttagta aatacagaac
     6781 acactacatt tagtgtctta aggagtcctc atacatgaag tcatattttc agtttttagc
     6841 tcatcagcta aaaggctggc cgcaacaaaa ttattatctt ttctttttca gtcttggctg
     6901 ccaggtcatg actcttgtca atagtcccat cacttggctg tccgttatta catttatcgg
     6961 taccacgctt ggtgtattgt gtattttatc catcaatgcg gcaaaatcag tcaacggtat
     7021 tttaggtatt ttgtctgcga tttgttttat tatcgttggg ttttcagcaa agaactattt
     7081 aagtatcggt gaacagattg cttatatgat cacattggat atccccgtat tgttaagtaa
     7141 agaatggaat cataatatgg cttcaaaaat ccgaaaattc actggaaaaa gctggtttat
     7201 cgcaatcatc agtactttcc ttgtctatct tgtttcagga ttcctgattg gtcgttatac
     7261 agatgatcct cggccgtggg tagatgccat tgctttttct attagcttga ctgctggtat
     7321 catcagtttc ttgcgttaca acaaccaata ttactggtgg ttagcttcag ggcttgctca
     7381 aatggtcctt tggtatattt catttacgca tggatctgca agtttggcga tgttcgtgaa
     7441 cagctctgtt tacttgctga atgatgtcct tgcatttacc gtttcacctt ggtataacca
     7501 aaaagaacgt gaacgtatgc aaaaaatgga agaagaatac tacaacgcac aacaacacgc
     7561 ataaaaaaac tgagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
     7621 tataaacggc ggaatcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg
     7681 aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
     7741 ctatttttta tagtaattcc ttcagatact ttcttgcctc atcattgaaa cgcagtgatt
     7801 cttctaactt ctgataatat tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt
     7861 ttcctttcca tttgtcgatt gtctcttgca catttccaga ttggatatct ttcactacta
     7921 gttgccattt ttcttttgag actgtacggt ctttcttatc agcagattgt atagcttctc
     7981 gttctttacg caatactttt aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga
     8041 acccttctgt aaaaccatag cgacaatatt gcttccaaat aaatagatag atttttggtt
     8101 ggatgaatcg ttggtttttc gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt
     8161 gacgttttgt ctttctcaac acggaaataa atgtttcaaa ataagcatta ggatgtactg
     8221 gcacttcttc atatacgatg gattgccaat ctttacgtaa aaatgcgcga tagttgatca
     8281 agaattcttc tacatccttt actaattctt ctgcaaagtc gtcaaatagt tcttttttta
     8341 tctgtcgctt ttttgactgc ccattgatgt gttcacttat tttcttgatt tcattttcca
     8401 atgcttgata atccgctatg taaatcggca tttctacttg gcgaaattct gcgagccgct
     8461 gagctaattt tatatccggt aaatatggct gtttcatccg acgattttgg tacaagacaa
     8521 aacagtattt ttgccatagt ccttcaggaa aatcgatatt cttctgctta agccataata
     8581 attcgtctaa ggccagatca acttttgtca ttccctgatg gatcgaagct gccactgtcg
     8641 gagagacatg caggtgatcg gcaaagtggt tgatgcctag ttttaggatc tttccggttt
     8701 ttggcgattt aacgatgtat tgcactttta attctctacc acactcacaa tacaattttt
     8761 gctggcttcg ttttgttcga aaaaacggat tgatcttgaa gtcagaaaac acccaatctg
     8821 ttgcagctaa ataatgttct tccataaaac gagagctgat tagatatcgg cggtgcttat
     8881 cgactagttc ccgctgtttt ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg
     8941 tgtattgttt agaaattttg atgggatagc ttttgctgat ttcttttgga aaccattctt
     9001 caaagacgga ttgccattct tccacggctg tttctttcct ttctaattac aaaaagctgg
     9061 ctactaacat aacgttagca accagccagt gtttcttgct aaattagtga gttgttccgg
     9121 catttccgga aatatcttct ttattttcta ctgctgcttc aactgcataa gtcaaagaag
     9181 aagcaatgtc ttctaaagac atagaaggcg tacccactgg acgatcgatc acttgatcgg
     9241 gtgagaatgg cacgtggatg aacccacctc gaattgccgg gaattgtgtt tcgatcaaat
     9301 gaagcaaacg atacatgata tcattgcaga caaatgttcc tgctgtataa gagatgtacg
     9361 ctggtagacc atgatcttgt acattttttg tcattgcttt gattggcaat gaggtaaagt
     9421 aagcggctgg accgttttct tctaaagccg tatcgaatgg ctgatttcct tcattatcag
     9481 ggattcttgc ttctgctaga ttgattgcga cacgttcgaa cgagactgct gaccgcccac
     9541 ctgcttgtcc gacacagatc acgacatctg gttggtgctg ttggatggct tctttgacta
     9601 cttgactgct tttttcgaat acggtcggta cttccacttt gatgatttca gcaccgctga
     9661 tttcatctgg catttttttt actgcttcat aggctggatt cactttgtcc ccaccaaatg
     9721 gatcaaaacc tgttactagt actttcatct gttgttcctc cgtccgattg atttaacata
     9781 tggttatctt ttatcatacc attttccttg aacagcgtaa ataaaggaaa caatagttta
     9841 aataacataa cagatcaaac ttaatatatt cttataaaaa aatagtgttc cactacaatg
     9901 ttagaagagg ttgcgacaat tattctgtca caacccctct cttctattat ttaaatagaa
     9961 tcatataagc aatttggaaa actagcataa ataacgcaat aaacaactgg tttttgatca
    10021 ctccgtattt gtctttcatc tccagcatcg ctacaggcac gatattgaag ttagcagcca
    10081 tcggtgtcag cagggtccca caaaaaccgc aagtcaatgc aaccattcct attaacgctg
    10141 gattcgctcc atgactaaat acgaatggtg caccgatccc aaccgtcatc acggtgatgg
    10201 ccgcaaatgc attccccatg attaccgtaa atatcaccat gccaagagcg taaatgatga
    10261 tgccgatgtt cacatttcct tctggaacga tcgtacctac tgcattggaa atcactgtcc
    10321 caactccagc gcttgtaaat accgcaccta gagaagctaa aagcattggc aacatgctta
    10381 acggccctac tgtgcccagc atatctgctg catcatctaa aaatgtactt ggtcgattat
    10441 ctcgagaaaa gaacatcaag attagcatgg ctgcaaaaac accgacgccc acgccgacta
    10501 aagcacctaa attggtaaat agagcaaaca agatcgcaaa gacaccgata ctcaaagctg
    10561 gaataaaaat tttcattcct agtttgtctg acattttttc catatattct tttgatggaa
    10621 gccggctttc tcctttgctg actttcttag caattgctgg tatggtcatc gcaaagatca
    10681 agccaccgtt cacgattgcc ggaagccatc ttcctcctgc aataactacc cctaagaccg
    10741 tccagaaaaa ggctgaccca taacgatgag gattggtttt atctgttaaa tttcttactc
    10801 cagtgtaaat caatacgagt cccatcacga tataaaggat ctccagtaat ttatcgccta
    10861 ataaaatatc aggatttgta aaaaagtcca tcttattgcc cctttcctgg ataatatttc
    10921 ttcgctaatt tcttttcttt aagatagaag tagacacttg ccacaactaa agccgtgata
    10981 gccactggaa cttccactaa agcaagctgt cccaatgtta cgtcatatcc tagatctttc
    11041 agtgttgatt ggactagcaa cgcccctgct cctccgatga atagtacttg gccaaagaac
    11101 cagcagacat tttccatagc tgaagccata ccttttagtt cttcttcatg cttaggatgg
    11161 acttctaacc cttttgattc gatggtcccc aatgccatcg gtaaaatgat tggacgaaca
    11221 aatccagcaa ctccaccaaa actcacgtta aaagccgcaa agatcattcg aaagatccca
    11281 taaatatcta caatcatacc agcagatact ttcttgaatc gcttgatcaa ggaagccgct
    11341 gattctttca gaccatttct ttcaagcgtt cctgttgcca acataatgat gataaaaatc
    11401 gccatcccgc gattgtttac aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca
    11461 agaccgccag tcaaagctgt aacaactaaa gcaaccatta cgatcaatat cgagtctaat
    11521 ttaaatgcaa acccgagaat aacaatcaag acgcctaaaa ggctaagata attttccata
    11581 tattttcctc cataaaatga caatattttt ttaattataa agcaaaacac ataaaaaata
    11641 tagttgtttt ctcacgaaat agacactatt tttaaatatt tttcgttatt tttttatcac
    11701 aaaaattatc ttacttattg taaggtaaag ctttttttaa tttattgccc gataatatat
    11761 agaaataagg tctagttact gtttttttta aatgattaat aaataacaac ttctttctat
    11821 gaaattgcaa agcattcatc ttcttttccg ctaacgatat atccgtcagt gcttggtaca
    11881 ctttactaaa aagtatgaga ttgccgatat ctggataggt gtgctatact acagaaaaag
    11941 atatacaatc tgccaccggg taggataaag aggatgatca ttccattagg tctttgaagg
    12001 gatgattttc ctctttttgt gtattttttt atagttaagg aggaatgtaa gtatggcatg
    12061 gatggaatta atcatagcag gaattttgga agtattctgg tcaacgatga tgaaatggag
    12121 tgatggtttt tctaaaatca attattcttt ttacacagtg attggcatga ttgctagttt
    12181 ttactttttg tcaaaggcaa tcaaaagttt gccgatgagt ctctcttatc ctatctggac
    12241 agggattggc gcagttggtt cggtcataat tggtgtggtc ttgtttcatg ataagctgaa
    12301 tctttccact tggttctttg tggggatgct actcatcagt atcattggca tcaagattac
    12361 tagcggccac tagacacttg gaataagtac tgatcagaaa aaataagcct ttttatttaa
    12421 tattcaattt ttttatgaaa aaataagaaa taagtccaat gacctagcga cttctccact
    12481 catcaggtgt atgcttgtac tatctataga aaaggagtga atatttttga aaagtctttc
    12541 ggtccaagaa ttatccaaga actttggtca caaacaagcc ctcagcgatg tcagttttag
    12601 cttatcccct ggcaaaatcg ttggattagt aggtcctaat ggcgctggaa aaacaacgat
    12661 catgaaagct attttaggac tgatccatta ctcagatgga acaatctgta ttggtgaaaa
    12721 tgagatttct ccctctagtt ataaagggtt ggaacaagta ggagcattga tcgaataccc
    12781 gggtatctat ccattcttaa caggctatga tcatctgaag ttattctctg aaaccaacga
    12841 tgtatctgct atcgacacta tcgtgaatca gctcaagatg gaaaagtaca ttcacaaaaa
    12901 agcgaaatcc tactcgttgg gaatgaaaca aaaattggga attgctcttg ccttactgaa
    12961 ccatccgtcc atcgttattt tagatgagcc gatgaacggt ttggatccac aagcaacaag
    13021 agatgtgcga aaaatcatta ccgacttggc tactcagggc acctctttct tgatctccag
    13081 tcacttactc agcgagcttg aaaaaatggt cgatgagctg atcctgatca atcaaggaaa
    13141 gatcatcaaa caatgtacaa tggctgaatt gaaccaaaca gcgcaagatt tcatcgttat
    13201 ctctacaaca aatgatctag ctgctcgaac gatcttgaca gaagctggat atactttgga
    13261 agatcctgat aaaatcaagc ttttaaaaga aaacgaagaa ttattaggaa ccgtgatcca
    13321 gctattgtct gcacaaaata tccatgtgac agacgtccag catctgacga atgatttgga
    13381 atcttctcta ttggaaattc tggattcaat aaagggggaa gaagaaaaat gacattgctt
    13441 gtaaaacaag aattatttaa attgatcaag aaaaagtcaa ctgctgtttt atctgttcta
    13501 ttagttgttt tgctcatagg aacagcactt ctagccaaaa aatacacgac gatcattgat
    13561 ccagttgaaa tgactgctca gctttttagt gccacatctt ggattgtctt tatcatgatt
    13621 gcagctgcaa gcacgatcat ttcgatggaa gcacaatatg gtacattgaa aaatctcctt
    13681 tatcgaaagt attctcgagg agaaatatta gtaagcaaat ggatcacatt agttatttat
    13741 tctgtttatt tatacctttt agctattatt gtcactgtgt tgatgaaact gattcttttc
    13801 ccttcgattt ccttcacgga gaaggtttct actggtcaaa cactgatcca aagcttgttc
    13861 acttatactt tgggtagtta tatcggttta tggctgattt taagtttagt gttgatgatt
    13921 gcttgcttta tcaatagttc cggcgcatcc atctcagcag gaatcgtctt ttattttgct
    13981 tcgtcaatca tatctggtat ccttattgca ctcatccaaa aatgggagtg gatcaaatgg
    14041 aatccgatca gtatgttgaa tctgcaaaac caaattggaa acgaagagat catgaagcaa
    14101 ttgactcatc tttctactaa tcaaatgcta ttcggtaatt tagcttacat cgttttgttc
    14161 ttagcattag gatatctcgt atttaagcga aaaaatgtat gatgactcat caaaaaagag
    14221 gctgtgaaag agacgttcag ccttattgcc gattggctgt ctcttaaaca ccgtttattc
    14281 tgaaaaagga gctgtgacaa catttgtgtc acagctcctt tcctagtatg tttttgaata
    14341 aacgacaggc tcacaacctc tctttatact tcttttatac ttctatacgt gaagcatccc
    14401 agtggcaaat aaaaccattg caatcactcc gaccatcgtc acacctataa gtaaaatctt
    14461 atctaagtgg atatgttgta caggctcgtt ttgctcctta cgtgctttga ggtaaaaata
    14521 aatgcctggc aagaaaagaa caagaacaag cattacttca ttaaaggcta cgactagcat
    14581 agcccatact tgaaaggcac ttgctaaaag ccctactaaa aattggaccc attctttccg
    14641 ctgataagaa agtttcattt ggtacaatcc taagaaaagc cacgtgaaga aaatagctgc
    14701 tgtaccaagt gtgtaagcaa agttataagc actgtcagtg aataataatg aaagtaaaaa
    14761 gacattcgta cataacgtcg tcaatacgat agcataagtt ggtgccccat ttttgttgag
    14821 ttttccccat gctttaggca aaagtccgtc ttcagccatg tttcccattg tttcaatcgg
    14881 taagatcgtc catgagagcc aaacaccaac taaagaaatg atcaaaccgc cgttgatcac
    14941 gactgctccc catttaccaa ccatcatttc aaacacatag cccatagcag gctgcgcctt
    15001 gatcgtttgc agctgattca tcgtcattgt cccatatgga agcatggaaa ccaagacgta
    15061 aataataatc aagccgaata tccctagaat cgttgctttc ccaacatctg attgtttttt
    15121 tgctcgtgaa cctacgacag aggctccttc aataccaaca aatacccaaa gcatcaccat
    15181 gatgcaactc aatacttgac tggaaaacgg gatcggctgt ccattcgcat tcaatataat
    15241 gtcgctagaa aagttatcaa tgaattgttg gatcgaaaac gtgctgatag ctactacaat
    15301 aaataaaaat aatggcacta atttacagaa tgtgacgata gcattgacga aactagcatt
    15361 ttcgactccc cgattcacta gccatgcata tccccataaa aagactgtac tcatcacgat
    15421 cgatgccaga ttttgcccac cttcaaatac tgggaagaaa tatcctaatg cgctcatcaa
    15481 caatgttgca aaagctacgt tgcctaacca gcatgtgagc cagtaacccc aaccgctgat
    15541 aaaaccagcg aaacggccaa aaccttcttc tgcgtaacta aagatcccgc cttttaaatc
    15601 tggacgtttt ttattgatat tattgatcga caaggagagc atcaacattg ctgttcctac
    15661 gagtacccaa ctgaaaatag ctgggccgcc agccgatccg gagatgtccg tcataatacc
    15721 aaatacgcca cctccaatcg ctgaaccaat tactaaggca gcaagtggaa ttaaaccaat
    15781 tccttttttc tgttgctctg tatccatgta agcatcctcc aaaataaaac cgcttttatc
    15841 aaaaagtata acaaaccttt tttattttta cttaattaat aaaaacttta atagaaatat
    15901 aattctaccg gcttttttct cattatacaa gcaaagcgcc tagtcccttt ccaaattaca
    15961 ccaccaaaaa acttaaaaaa gatgctatac taaaaggaaa gaattggagg atgatccatg
    16021 aacattttat atatatcgat ttcaggaaat acgcgtgcct ttgccaaaca tctagcagaa
    16081 tatgctgaaa aaatgcatgc agaagatcct gtcaatcctg aagttacttt aaaagaaatc
    16141 catgaaaata gtgattttac gaaagaaaca gaaccatttt ttactttcgt tcctacttat
    16201 ttagacggcg gaaatggttt agataacggt gatacagaaa tcttgaccga aacgatgcgt
    16261 gaatatctgg aacatgaaga caatcataaa ctttgtcttg gtgtagtcgg tagcggaaat
    16321 aaaaacttca ataatcagta ttgtctaaca gccaaacaat atgcacaaac atttggcttt
    16381 cctttcttgg cagattatga attacgggga acaccatctg atgtgacacg aatttatcag
    16441 attttagcag aaaacaataa ataataaaaa agaagaagct gaaaaataga tttcagtttc
    16501 ttctttcgtt tctattgttt ctatcatatt aagctttgac atccatcaca ggtgcttttg
    16561 cttttgcttt acctgttttt tccaagacaa agttttttac acgatccata ttggttacca
    16621 agacgatcat ggatgtttct ttgcctgctg ctttgatcgc ttctaagtca gcttctgcta
    16681 caagcgtatc aggtgtcact tgctgccctt ctgttacttt cacatcaaat ggcgtaccgc
    16741 ctaagtcaac tgtgttgatt cccatatgaa gcaagatttc taaaccattt ggcgttgtga
    16801 tcccaatagc gtgtttcgtt gggaagacag agctgactac cccagtaact ggagaataga
    16861 tatgtccgtt ttctggttct acaaagtagc cttcacccat catcttttgt gcaaaaactg
    16921 gatcacttgc tttatctaaa gaaacaactg ttccgtcagc aactgatgag aggatagtca
    16981 cactttcagc tgaagtttga ggtactactt tttcaggcac ttcatttact gtggctgttt
    17041 cagtcgctaa ttcttctttt tctaaacggt ctttaggaat accgaagaag aatgtagcaa
    17101 cgaagccacc agcataagct gccaataatc caaggatgta gcctaaccat ttaccgtcag
    17161 agatcaatgg gatcaaagcg gctcctgaag gtccaatagc gatagccccg acatttccga
    17221 tcattccgat cactgccccg ccgatacctc caccgataca agcagtaata aatgggcgtc
    17281 ctagaggtaa tgttactccg taaatcaatg gttcgccgat tcctaggatc ccgactggta
    17341 aagctccttt gatcatttct gttaattctt tatctttttt gcagcgaatc cataatgcca
    17401 atgctgcacc cacttgtcct gcaccagcca tcgcaagaat tggaagcaat agcgtacgac
    17461 cagtttcatc aatcatttgc acatggattg gagtcaaaat ttgatgcagt ccgaacataa
    17521 ccatcggcaa gaataatgtg cctagaacga agccggcaaa tgctccacca acgccaagta
    17581 cccagttgat cccaccgacc atgccatcag aaatgaaacc tgctaaaggc atgatgaaga
    17641 aaatttcaat cagaccgatg gcaatcaatg aaagagttgg tgtaacgatg atatctaccg
    17701 catcaggtac tactttatgc agttttttct caacgattga caatagccaa acagcgaaga
    17761 tgacacctag gatcccacct tgtccagctg caagagcttc accagtaaat aagtttttca
    17821 gtggtgcttc cggattcatc cctgtcagca tcacaacggc cccgataact ccaccaagag
    17881 ttggtgttgc accaaaaact tgtgcagcat tgatacctgt atagataact aggtagctaa
    17941 acatcccgtt tttcaagatg ttcatcacat cgatatattg ttgccatgtt gctgcatcaa
    18001 gtgttccggc tgtgactaag tttgctaaaa ctgcggcaat ccctgccacg atcccagtac
    18061 cgacaaatgc aggaatcatt ggcacaaaga tattagaaat gtcttttaat accgatttga
    18121 ttttagatgg tttttgtttc gatttttgtg ccgctttcat ttcttgtgcc cgttcgttta
    18181 cacggtcttt tccagatgct gatgaatttg ctgtagctgt acttacagaa gaaagttttt
    18241 ctcctaattt cacgcctgcc aaatcaacca tttgctgtgc tactttgttc actttcccag
    18301 gtccgatgat gacttgaagc tgttcatctt ctactacacc aagcactcct ggaatcgctt
    18361 ttaattcaga gacatttaca agatcatcat tgatgatctt catacgcaca cgagtcatgc
    18421 agtggacaat gctttctaca ttgcccatgc cgcctgcccc tttataaatt tgttttgcca
    18481 gttcttccaa agtcagttcg ttttttgcca tggtcttttc cccctcataa ggtccctttt
    18541 acaaatccgt ttgcttcagc taattttgct tgagcttcct ctttatcact atttgtcaaa
    18601 atcatgacga tagccagctt aacattttga tctgcttctt caaagtactt ttcagctgtt
    18661 tcgtaatcag cttctgtcgc ttgcatgatg attcgttttg agcgttcgat tagtttttca
    18721 tttgttggtt tgacatcgac catcaagtta ttgaatactt tacctatacc aatcatactg
    18781 atagtagaaa tcatattcaa gataagtttt tgtgctgttc ctgacttcaa tcgagtagag
    18841 cctgtcaaaa attctggacc tgcatcgact tcgattggga aatccgcgtg tttactgatt
    18901 tcggcttgtt tattgcaaga aatcgttcct gtgacagcac cgatcgattt tgcgtaatct
    18961 aatccaccga tcacatatgg tgtacgaccg ctagctgcga taccaataac tatatcattt
    19021 tttgtcaaat tcagcttgat tagatcttct tgccctagct cataagaatc ttcggcccct
    19081 tcgacagcta cagtcattgc ttcttctcca ccagcaatta gacctatgac catttccggt
    19141 tctacaccga atgtcggtac gcattcagca gcatctaaca cacctagtct tccacttgtt
    19201 ccggctccca tataaatcag acgtccacct tttttaaagg actcaatcgt gttagcaatc
    19261 actggttcga tacttgtcag ttctttttca accgcagcgg ccactttctg atcttctttg
    19321 ttcattagtt gaacggcttg agaaatactc atctcatcta atccaaaagt ttcttggttt
    19381 ctgcgttccg ttgttaggtt ctctaaattg attttgctca tttttatctt ccttcttttc
    19441 ttataaattg atagttcata ccagctttga tttggttgat caatggtagc tcttctttga
    19501 taactttagc tactacattc actttttcat ccgctggcaa atcatatttg gttaattgga
    19561 tctcacccat atagcgcaaa tacttttcgt tatctaaagt aatagaccct ttcatacgcg
    19621 tagctgtatt tcttgctggg ataggcggaa tctctcgaaa acgtgcatct acgcttcgta
    19681 tgacatctct tgcatcgtct tgccgattcg tatgcttccc taatatatat tcgtaaaatt
    19741 cttcatctac aggttccgta tggagcaaga ctgtctgttc tttttggaaa gaagcaaatt
    19801 gctcctgaac gttttctgac agacctgcat ctccgatgta aacaatatcc gtgttacttg
    19861 ctaatagatc tagagcgcct gcaagaggat ggatccctcg atgttcttcc aaagttggca
    19921 atccttgata tagtgggcct ctcaactctg tatctcctgg tacaaaacct tggacaagga
    19981 gaccttgaat tttgagccat tgattttttt tctgatacca ttctttgtcc agccctgttt
    20041 cgggacgtgg ataataatta tgccaagctt caatctgtga gaaatcagct tctgcttctt
    20101 tcaattcatc gatatcttta ggcgtgatcg tgctagcatt caaactgatt ttcaattgat
    20161 gtgaccagtc ggcaatctgc tgattggaaa tatgataatc cattcttaaa cctgttacac
    20221 caattgcttt caaaggcgtt agatcttcta atgaaaaacc cgctcgttcc aatgcctctc
    20281 ctgaaatatc gaccatcaat tctagtttat tctttttcgc ttcgcttcct agttcgatca
    20341 atcgcttttg atatgccgaa acatcatctt ctggtatatg catggaagtg aaaataccga
    20401 caaagccatt ggatgccatt tggcggatat actgtttctt ctcttctgac aaagaatcgt
    20461 tcataaatat tgaaaagcca aacatattat ccctcttttt ctgtatccgt cttcatttct
    20521 atgactagag tatatatcta aagaaacaaa atttcaatac ttttattaaa ataattaaat
    20581 ttcatttcta aaggttgagt ttatattatc aaaagaattg tcataaccta aaaaaggcac
    20641 tgctaagcaa aagcagtgcc ttgaaatatt ttatttaaat aacacaaaaa cataattact
    20701 cataagaaac caattctgta ttatctacca tgaatgtaaa gaacccctca ttaggcatcg
    20761 gatgaaaata agcattgatc actcttatca aggcattatg gcaaactaac agatagacat
    20821 tttcttcgtc ttcgatgcat tctttcaata aaggaacgat ccgtgcatac acatctaaga
    20881 ctgactcccc atttgggaat cgtacagcga acgctaaacg tgctttttga aagttctcat
    20941 ctttgcttgg cagtccatca taatccccaa aatccatttc taccaatcgt tcatcaacgg
    21001 tcagcggcaa gctgagacga tcagcaactg cttgtgctgt atctcttgct cgttgaagag
    21061 gggaatggat gattttcgtg attggaacag gcaaatctac taccttttct gctagtttct
    21121 gtgcctgcat ctgtcctttt tcagtcagcg gcacatctgc ccgtccgcaa actaatcctt
    21181 gtgcgttcca agttgtttct ccgtgtctcg ttacataaag catgtgattc tctccttcgt
    21241 tggcaaagtt ccccttaacc ataccttgtt gttcttgaga ttacaagggt tagattctct
    21301 caagagaaat aatctcagta gatactttat ccaggaaggt ttgatcatgt tccactagta
    21361 acatggcagg tttcacgttc aagatcagtt gttctaattg ttcttgattg aagacatcca
    21421 aataattcaa tggttcatcc catgtatata gttcagctgg ctgtgataaa gatttagcca
    21481 attccacttt tttccgttgg cccatactca tctgctcgat cttgttatga aaaacatctc
    21541 tttccatccc aagctttcgg aggttgttca aaaatgcttg gtagtctact tggtttttct
    21601 ctgcaaattc cgccaacgtt cctcgattgt cttcataatt ttggcttgca taactaatgc
    21661 tcagatgttt tggctggtat ttttctccta taaccttgcc gttgaatgcc cccagaagat
    21721 aatggataat ggatgacttt cctgcaccgt taggacctga aatcgccacc cgctgatgag
    21781 gctcgattgt aaaatgaatt ggctcgaata acagattttc ataccctaat tgaagatctt
    21841 ctacgcttaa aagtcgctta tggtgagacg cttggctatt catcgtcaaa gaatcgatat
    21901 attcgatatc ttttaatagt ttttctttct cgctgatctg ggtctccatc cgattcacga
    21961 ttgctttgga tcgtttcatc gtccgtgcag catcagcacc cactgctcct ttattcactc
    22021 gtctagattc agtatcgatg aatccgactt gtttctttgt tttatctcct tctcgggaac
    22081 gagaccattc ggctttttca gctgctgttt tctttagcct actgacttct tttttcaatt
    22141 tttcattttg agccatttca aattcatcac gaagtttttt ctgttcttca tagatagaga
    22201 aattcccttg ataaagttcc agttgacttt tttcgattgc taaaacatgg tccactactt
    22261 catcgataaa tccccggtca tggctgacca cgatgaagcc ttgttttttc tttttcaaat
    22321 aagccgctac ttgttttcta ccagagatat ccaaatgatt cgttggttca tcgattaacg
    22381 ggaaatgagt gtcatccaca aataaaagtg ccagtaggac ttttgtcttc tctccccccg
    22441 atagtgtgct gaattctctc cataagattt caggatctgt ttgcatcaat tggagctctc
    22501 tttcgatttc ccatatctca aaatccgtaa tatcatttaa cacgtaatag gttaaacgtt
    22561 ctttatcttt tgtctgttgc gggaaatagg caaattcttg ctgatggatt acttgccctt
    22621 gataaggtag tttgttttgt agaatattca gtaaagttgt ctttcctcga ccgtttcgtc
    22681 cgataagtcc tagtttccat tgtgtgtcaa aatttagatt tgcttgttca aataataatg
    22741 tgccttggct gtcgtagccg aatgtcagat tttttatttc gattttagac attttccatc
    22801 acctcgtaaa aaaagaccag tcctattttc agaactggct aaagaggatg caagggttgg
    22861 atacacacaa aaagacccat acatcgaaaa aaccaagcca atcccgaaaa tctgcacact
    22921 gcttccttgt ctccacaagg tacagtctga atgcagcttt catcaatcga cttaattttt
    22981 caatgcacgg atccctcact ttgtcctata tttgaaatta cgatagcata aagaaaatga
    23041 ttttgtcaac tacttcggga ggatggtgaa aaatctgcta agtgtctgtc ctaagatttt
    23101 tgttttttct aatgtttgcc tgtgttccat tgcttcttct aaacctattg gacccaaatg
    23161 aatacttaag acaattggga gaaaatcaga aagtccttct agctcttggt ctctacttcc
    23221 gcaaatagcg attattggtt ttcgatattt ttttgcaagc actgctacac cgtatggaac
    23281 ttttccgtgg atcgtttgct gatcgatccg cccttctcct gtaatgatga catctgcttg
    23341 ttcgatcttt ttttccaatc cagtgatttg gctgactaaa tcgaaaccag aaaccatctt
    23401 cgctcccaac aaaaaaaggg ctccaccaat accgcctgct gctccacttc ctggaatatc
    23461 atctaaatcc acctcccaga aatcttttat ttgctgagca atctttcctg cttgtttatc
    23521 taagaaagct agcgtcgtat ctattcctcc tttttgtctt ccaaattgcc aagcagctcc
    23581 ttgttgacct gtatatggat tggtaacatc tgcagcgata atcaatttta ctttagaaac
    23641 aagaacgttt tttcgatcaa aatgaagttt tttagtagat aataaaggat ttccttctgt
    23701 gtacccttct aaagaagcac ccaaggattg gagtaatcct agaccaccat cgctacaccc
    23761 gcttcctcct aaagtgacaa taatttgttc tacttcttga agcagtgcat ctttaataag
    23821 ttctcctaag gcataactgg aagcaagtcg gatagtttga tccgttggtt tatcaagtag
    23881 atctaacccc aatgcttgtg cagattcgat gactgcggtg ttttttcctt gccaagttgt
    23941 caataaatag ctgatttttc gctgacggaa taagagatcc atcccatcaa tggtacgcca
    24001 tgttcccctg agcgcgtaat agattgcttc tgcagtattt tctccgccat ctgcaatcgg
    24061 cacattgatt tttcttcctt tacgatcttc ccagccattt agaactgcct gatttagttc
    24121 aaatgaagaa gcacttcctt taaatgaatc aattgccgcc aaaatatcca tctgtatccc
    24181 tcactttctt cttaagtaag acactttttt tcattttttt caaacaaaac aatgctattt
    24241 tcttccattt ttctatatac taaaagggaa ctcattgaat aaaggagaga aaaaacatga
    24301 aaatcgcaat agctggggcc ggagcaatgg gcagccgaat cggattgatg ctacatcaaa
    24361 gcggaaatga ggtcctcttg atcgatcgtt ggcctgctca cattgaagcc attcgtacaa
    24421 atgggttgat tgctgacttt aacggtaaag aagtggtcgc aaaactgcca atctattcac
    24481 cagaagaaat catcgaaagc aacgaacacg tggacttgat cgtagcatta acaaaagcca
    24541 atcaattaga tgatatgttt tgttcgatcc aatccattat tactgacaat acttatgttt
    24601 tgtgtttact aaatggatta ggtcatgaag atgtactgga aaaatatgtg ccaaagaaaa
    24661 atatcttatt tgggatcacg atgtggacag ctggtctagc tggtcccgga aaagtcacat
    24721 tgttaggtga tggagaaatc gaactagaga acttggaacc agaaggcgaa gcatttacaa
    24781 aaaaagttgt agaagtcttt caagaagcaa atttaaatcc gatctatagc cataacgttc
    24841 gttattccat ttggcgaaaa gcttgtgtaa atggaacatt gaatggttta tgcacgattt
    24901 tggattgtaa tattgctgaa ctaggcgcgc aaaaagcggc agagagcatg gttcgaacaa
    24961 tcgtatctga atttgcttct atcgcagcaa aagaaggaat catactagat caagaagaag
    25021 tgtaccaaca tattgcttct acttatgatc ctgacaacat cgggttgcat tacccatcta
    25081 tgtaccaaga cctgatcaaa aaccatcgtt tgactgaaat cgactacatc aatggtgcca
    25141 tttggcgaaa aggacaaaaa tatgatattg ctacaccata ttgtgccttt ttgacccaat
    25201 tagtccatgc aaaagaagga atccttggcg cagaataaaa ggctctctga tgagaatcag
    25261 ctgaatcagc agctggttct ctttttttaa gcccaataaa agatcaaaaa gaaaacacaa
    25321 ctctcatgat gccataagaa ttgtgcttgt ttgtttcagt acataatctc ttcaaaatgt
    25381 aggtttttta tttgttttca acagaggaga ggtcatattc gtattgctca taaatagtgg
    25441 aatcgttagt gataaatcgt tttgcccaat tttctgcatc ataaaattcc aatagctctg
    25501 ataggattgg cttaaatggt gtatgcagaa attccggtaa ttcatctaaa tcacgttctc
    25561 gaagcaaaaa aatattttca ggacgataaa aatgataatg agcaatcgct tgattatccg
    25621 tcactagttt cttttgaaaa tacatggatt caaatgtccg caaagtcact ccttgctgac
    25681 cctcccgcaa cagttccaga atcccgcgcg attcccctgt ttttttcagg tactcttcat
    25741 aagtaagata tccaggttgt tcattccctc gatgtggaag aatgaaatag ttggtcgtaa
    25801 catctctttt tataaattcc tgcttatagc tttctgctcg ttcttttctc ccattattcg
    25861 ttgcaccaaa aaatagatcc gtctttggtt cgctagaaga aaaattcata tgtttcgaat
    25921 aaaatgaact attatgtttc agcccaagtt cttttgcttc caaaaaatca aaatgataaa
    25981 tctcatcgat tgccggttca tcttgtgcct gcttcagcat cgcctcgtgt tctgctttga
    26041 agtgattcca aaaaaataac acaatttttg cattggtaaa tgggcgcacg cgtcgagcca
    26101 tctccatatc aaatccataa tctaaaaaaa cgatcagctg ataatttgcc aactgttgtc
    26161 tagctgattt aatgaattcc gactgtagta aatcttttcc ggaccaaaca tctaaagagc
    26221 ggatggaagg caaaaaataa agtgatttat tttttgcaac tagcagtacg tcatttttgt
    26281 tcatcttttc cgcctcactt tctctgtcac tttttcctat gatagcctgt ttaaatagta
    26341 aaaagccagt aaaacgtttc ctatatttgt atgaaaattt aacatttgta tgaaaatatg
    26401 atgaaataaa tcattcttct attaaaattc gcctaaaagt tttctgaata gttgtaattg
    26461 tctgaaaatt tgttatcatt agtcattgtt aaatattgct atttattctt aatataaatt
    26521 ttgattgtga atttaaaaga aagaggtcat ttatggaaga taaaaaattg aaaaaacaac
    26581 ttagctccag acatatcacc atgttagctt taggtggagc aatcggtgca ggtctattta
    26641 aaggaagtgg cgaagcgatt gggatcgccg gcccttctgt attgattgct tttttgattg
    26701 gtgggctgat tttatatatc gtgatgaaag gtctgggaaa aatcgtttta agcggtggcg
    26761 acacgcatca cggattatct ggtttgatac gcccttatct aggtgctcat tctgctgatt
    26821 ttactgattg ggtctactgg tctatgtgga ttatcaatat catcgctgaa gctgttgcag
    26881 ctgcttcctt tttgcagttg tggttcccgc acattccagc ctgggtattt gtttttatgt
    26941 tggctgtttt aactacgatc atcaatcttt attcggtacg gttattcgca gaaacagaat
    27001 attggctggc ttttgctaaa atcagtgtca ttatcttact gatcattttt gctacttatc
    27061 ttgtcggtca gcaaatgttg ggcacaggcg ttttccctac ccttcagcaa ttgactgacc
    27121 atggtgggtt tactcctcat ggtatgaagg gaatcatcag ttctttgctt gtagttatct
    27181 attcttacgg cggttctgaa ctgattgcta tcacggtaag cgaagcagat gatcctaaac
    27241 gtgcgattcc taaagcaatc aaaggtgtca tgggaagaat catttccttt tacatcattc
    27301 ctttgttttt gcttttgatt ctttttcctt ggaatacttt agcaggcacg aatgtcagtc
    27361 cttttgtgat ggtatttgaa aaaatgaata tcccatttgc agcggatatc gtcaattttg
    27421 tgattgtcct tgctttgttc tcatctatca attcgggtgt gtatgcatct tcaagaattt
    27481 tgttcttccg tttgaaggat cgaaaaggtt cctctagaaa actagctgtt ttaaacaagc
    27541 atcaagtacc gcaacgtgct gttttatttt gtaccagtgt tctttatctt ggtgtagctc
    27601 tttcttattt tgtcggtgac aaattatttg gctatctggc aggttccctt tcgtatacag
    27661 tattattgat ctggatcatt atcagtgcgg catcctttgt actggcacta agaaaaggaa
    27721 cattatggga taaaggaatc agcttttttg cattagctgt cttaggattg attttccttg
    27781 gtatcctgtt cacgaactcc attggggtaa ctgtattgac tggcctgctt tacctgttca
    27841 tttatttcag ttatcaaaaa aagaatgatg cgttcgtatt gacgaatgaa tgaaatcgat
    27901 agaaaatttt attttattta tgtggaatag atggagctat tgaagaaagg agttgtgaca
    27961 aaagtcttgt tgcaactcct ttttccgaat aaacagtctc atgacctctt ggtaaacggt
    28021 gttcaaaaac cctctgtgca aaataattga ttgttctcca tgctcatgac atgatagaag
    28081 aaaggaatga aaaaaaggag tttgttttaa atgaaaaagt attggaagtt tttgttgatg
    28141 atcggtgttt caacggtagt tatgtttggt atgatgtact tgaatgtcta tgcgctcgat
    28201 catatcttct tcagtcgtac acgagtattc atggcgttga tgatgggtgc agtcatggca
    28261 gtcatcatgc tgttgttcat gtggaaaatg tatgaaaaca aaaagataaa tgttatgatc
    28321 ttagccgcta gtcttcttgt ttttgccggt tccttgttta tggtacgaag ccaaactact
    28381 gtcaacgaca cagcttggat gaaggccatg attcctcatc attctatcgc tatcctggca
    28441 agtaaacgag ctgatttaaa agatcctgaa gtaaaaaaat tagcggaaga aatcattcag
    28501 gcacaagaag aagagatcga caaaatgaaa aaaatgatca aagaaatgga atgaccatta
    28561 tccgtccaaa taaaaaaaga ggatgttcca gaagttatca gcttcaagaa ataagaagaa
    28621 gctacttctg gaacgccgtg aataaggggt tgtgaaaaag ctgccctgca tcaagtaata
    28681 agaacagcca aacaaaaata gcttctccta tttttcgttt ggttaggctt attaccgcag
    28741 atgcaagctt ttgaacaccg tttattaaga ggttgtaaaa tcagcgttct gacctgagta
    28801 ttagagagac aaatggaaaa atagttcctc atattttgcc attttttgag ctaataccga
    28861 gggtcagctg attgaacacc gtttatcgag aggttgtgaa aaagtcgctt agtttcgcgt
    28921 attaagaaaa attttgtgaa atggctttcc acatttttgt ataaatttgg cttaataccg
    28981 agaaactggc ttttgaatac cgtttattcg taaaagggat tgtgacaaaa gtcttgtcac
    29041 aatccctttt tatgttttta ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt
    29101 ttcacaaatt tttttgagat tccccaacct gtgaaggcac tgaccatcga aaacacagga
    29161 gaaagcaaac tgaaaaatac gaatggcaag taagtgagtg taggaatacc aaatgtactt
    29221 gctacgaaac ttccggcaat tccccaaggc actaagtagt tgattactgt tccaccatct
    29281 tctagcgttc tacctaatac agttggatca aatcctgctt tttcataagt ttctttaaat
    29341 gcattaccag gcagaataac agataggaat tgttcaccaa taaaaatatt tactccgata
    29401 cttgtcatca atgttgtaaa aatcaaattt ggtgtgttcg tcatctttct tgatactcgt
    29461 tccatcacgg ttgagatcaa gccaaattcg atcaacaatc cacctaatga caaagttaga
    29521 ataatcaatg acacagtagg catcatactt tcgattcctc cgcggctaag cagtgaatcg
    29581 atttgttggt ttccagtatt tgaaacaaac cctgattcga tgaccccagc caacttcgtt
    29641 aaggaagttt gtgggttttg aataaaaatc attcctactg ctacagcgat attcaacaaa
    29701 atcgttaaaa ttgccggcac ctttttccat gcacacagta acattaaaaa taaaggaatc
    29761 agtgaccaga ttgaaatagt aaagtttgct tctagaacat cagtcacttc acgaatagat
    29821 gaaagacttg cggctttact tgtgtgtcct aaaatcatga ataaaaagaa agaaacaaca
    29881 aatgccggga cagttgacca catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca
    29941 atagctgctg ctagatttgt tgattcggat aatggagaca tcttatctcc aaaaacggca
    30001 cctgaaacaa tagctccaac aaccagcgct ggatttaagc ctagtgttgt cccaataccg
    30061 aaaaatgcaa tcccaatcgt tgacataaca gtaaaggcac tccctacagc gctacctacg
    30121 attgcacaca caacaaatac agaagggaca aaccactgga cactgatcaa tttgaaccca
    30181 atgaccatta gagttggaat gatccctgct tgtatccata cagcaatcaa tgaaccgact
    30241 aaaataaaaa taaagattgg gatgattcct ggtttgatgc cattgatgat tccttcgtgc
    30301 agccaatctg tcgggtatct ttttatcact gcatacaaca tgaccattcc gatcgctgct
    30361 aagatcgtga tattaggtga gacctttaat ccgacaacgc caatcgcaat gcctaaaaca
    30421 ataaccatta atacaagtgt tgcttctaaa aaacttggtg ttcctttttt tcccatgata
    30481 atctctcctt ttttagttgt tcttatgttc taaaaaagtt aagatatcgg attactccgc
    30541 cgcaattcat ccgcgattcc tcctttataa aaataaaaag acctgtttta aactaaggac
    30601 gaatgactcc gcggtaccac cttaattaaa aacagatctg tttttcactt tcaaactttt
    30661 attcaaatgt atggactttc catgtaattc gtcaattagg cttgatcggt tcacagcgac
    30721 caccgacttt ctgaacaaaa cacctaaccg ctactgcgtg aaaagttgac ttgtacgaag
    30781 tttaccttcc ataagatcat ttgtcaatga aaaagaaaat attttttcat ttttctgaaa
    30841 attattttta taactttcac acacaagttt attttttact agaaagtagc gccctcttgt
    30901 ttttttcttg ctactatttc ttgatgttct tgcttgattc tttctaatag ctttggctct
    30961 ttcaacagat caagagctgt attggcgagt agttctgctg caatcgcaat agaagcaagg
    31021 cctttgtcac ttttagcagc agctttgaat tcttctgtgt gtccggcaat atatgtctca
    31081 gagattgcta ccgtaggctg gattgttggt atgacttggc tgacatttcc tacatcagaa
    31141 gaaccgagac ttgttctttg ttccgtttct atttcttcat ctggaacacc tgcttctttt
    31201 gcatgctgga agaataagtc atcaaaagaa ggagtaacga tgacatcatc aactgcattt
    31261 tggaacaatc caaactcaaa agtcgtgccc gtacttatcg cagcacctcg tacgatattt
    31321 tccacttttt catatacttt atctaatgtt tgccgatttc ctgcacgtaa atagaaacgt
    31381 ccagcagcgt aatctggaat gacgttggca gctactcctc cgtctgtaat gatcccatgg
    31441 atattgacat cttttggcaa ttgcaagcgt agagcgttga ttccattaaa cacttggatc
    31501 aatgcttcca aagcatttac tcctttttca ggtgctgctg ctgcatggga agcgacaccg
    31561 taaaatttga tgtcgactgg atcattcgct aaagattctg tcgttttccc ataacgaaaa
    31621 gctggatgga cgcataacgc tacatcgaca tcctcaaaaa aaccttctcg aacaaagctg
    31681 cctttagctg atccgttctc gcccccttct tcccctgggg tgccatatac acggatttct
    31741 ccaccagttt catcaatgaa ttgcttcact atgctggcgg ctaatacgga gtaagtacca
    31801 aacaaattat gcccgcatgc atgaccaata cctggtaaag catcgaattc tgccaaaaag
    31861 gcaattgtgg gaccgggttt attggaaata taacgtgcat caaaggctgt tcggtgtcca
    31921 gctacttctt tttctaccca aaacctttct ttttctaatt gtttaatcaa cgctgctgag
    31981 gaataaaact catagttact tacttcagga tgttcgtgga tatctagagc aagtgcttga
    32041 tattctggta aacgatattg gattagatcc ttcaattggt ttgttgagat tgttgttgtc
    32101 ataaaaaatt cctcctatgc tagtcatttt tcttattttc tcccgaatgt ttcccatgca
    32161 ggcacgcttg atccttttga tgttttctcg attactttga ctgtgtcttc tgtttgataa
    32221 gcgtctacta ttttttgata cgttttattg ttttcatctt cttttcttgc aacgatgata
    32281 ttcacataag gacgtgcatg gtcatccact ggttctaaga agatggcatc agaagcagga
    32341 ttaaatccag catctacagc tacaccgcta ttgatcactg aaatgtctac atctgacaaa
    32401 gcacgtgctg tttgggaagc atctaattca gaaatgtcta gattttttct attctcggta
    32461 atatcagaaa cagtgggcgc ttgtcctttt gccttatcca ctttgatcaa tccagcagtt
    32521 tgcaataaca gaagagctct gccgccattt gtaacatcat tagggattgc tacttttgct
    32581 ccgtctttga tatcagctac atccttcaaa gtgttagaat aaattcccag cggtgcattg
    32641 acagtatttc cgatcgatac taaatcagtt ccgtgttctt tattgtaatt gtctaaaaag
    32701 aattgatgtt ggaaagaatt caaatcaatc gatccatctg ctaaagccac atttggttga
    32761 ttgtaatctg taaatttcac gtattccaaa ttaatgttct cctttttcaa acggtctttc
    32821 acgttgtccc atacatctgt atcttctcca ataatcccta gttttacgat agtttctttt
    32881 ttgtcagttg tatctgcttt tccttggcta caccctgcca accaaataat tgctacaaat
    32941 gtaatgacta caaaacgaat aatttttttc attttttctc ctccactttc aatgtgtaat
    33001 ttttttaatc agttgattac tgataaattg acttaaaaaa actaagatta gaatcaataa
    33061 tgtagccaca atcgttacat ctgtttggaa tcgattatac cctctcgata tagcaagatt
    33121 ccctaatccg cctgcaccta ctgctccagc catggtagtc agtccgatca cgttgatgat
    33181 agtcaacgcc gagacccgaa tgataccagg caaaccttct aacaaataca cacgtaaaat
    33241 gataccgatt ggactggttc ccatcgcttc tgccgcttcg atcaccccag gatcaacttc
    33301 tagcagtgcg ttctcgattt gacgagcaaa aaacggaaca actccaaaaa tcaatggcac
    33361 taaagctgct ttcgcaccaa tcgtcgttcc aactaagaaa cgagtagtca atgctaaaag
    33421 agaaagcaag ataatgaaag ggatggaacg aatgatattg atcagcttat caagtatttc
    33481 aaatagcaga cggttagcta gtaatccatt agggcgtgtc actactaacg tcacacctaa
    33541 gatgattcca atcatcccgc caatgaatgc tgtccaaaaa gacatatata gtgtttgctt
    33601 cgtcgcttcg acaaattcat ccggaatcaa ataaacattg ggcaaatatg ttttaaagaa
    33661 atcagtcatt ttatcttcct ccttcgatca ctttgatacg actatctaaa tcgttttttt
    33721 gtaaaatatt tatttttaca ccagatttga tcaaataatc caatgctttt ttccgctgtt
    33781 tggtttctcc agctaaagta acgatcaaac tccctaacgg cgtttcttgt aataactcta
    33841 cattgccata taaaatattc gctgttactt gatatttgct gtacagatga gcgattagag
    33901 gttcgttcgt ctgatttcca atataactta attccaccag ccatttattg gcgatctgat
    33961 cggcaaaagc agaatgggca atgatcgttt ctaatgcttg gtcaagatga gtagctgttc
    34021 gaataaaatc tttggtcaat tcttcttcag gattgctgaa gatctggatg ctgctacctt
    34081 tttcaacgat tcttccatct tccataaccg ctaccttgtt gcagatttct ttgacaactt
    34141 gcatctcatg agtgatcagg acgattgtta aacctaactg gcgattcaat ttttttaaca
    34201 atgcaaggat ctgaagggtc gttttcggat caagtgcact ggttgcttca tcacataata
    34261 ataccttagg ttcattcgcc aaagctcgtg cgattgctac tcgttgtttt tgtccccccg
    34321 acagttgttt aggataagca gatgcttttt cttccaaacc gacaagttcc agtagttcat
    34381 tcactttttg acgacgttcc tgtttcgatt ttccagaata ctttaaagaa aaatcaacgt
    34441 tgtcaaaaat agttcggctt tccattagat tgaaatgctg aaagatcata ccgatggcct
    34501 ttctttcttt tcgcaattct ttgggagaga gctgactcag attcgtctga ttgacaatga
    34561 cttctcctgc tgttggtttc tgcagcaaat tcatcacacg aactaacgta cttttccctg
    34621 cacctgagta accgacgatt ccataaacat cgcctttttc aatcgttaaa tctacagaat
    34681 ctactgcctg tatctctttt tctttttgtt tgaaacatac agaaacatct cgcaattcaa
    34741 tcattaaaat tcctccttat ctaaaaaaag cgtcctcaag aaaatgaaaa tgtcattttc
    34801 ttgaggacgc ttttttgcgt gttaccacct catgttcaag ctgtgttcac acacaactcc
    34861 tcttcaagta cgatcatact ctagcaagat atcggtcgca gccgtcatcg ccttacatct
    34921 atgctcggga tgaattgctc gatggccatt ttccaatagt cgttccttgc ttcttctcag
    34981 ctaccgaagc tctctgtcaa aggttcttct atcgtactta ccatttcaat gcaaataata
    35041 ttcaaaaaaa aagtcctttt actaacataa actatgtcag taaaaggacg acgtatcgcg
    35101 gtaccacctt ttttcggaag tttccttccc tcaagcccaa catctcaaaa agagagattg
    35161 gcgttcgttt taacggtcga acagttccga atttgcctac ttatcgacaa attattctcc
    35221 aaggccattt tcagcttttc ttacttgact cttctcacca gccagagcct ctctgcaaag
    35281 tttccaaact tactttcctt ttcaacgaaa tcgatatttt gttgttggga ttaatctaca
    35341 tgaggaaaac tgaaaagtca agtacgaaat tcaaattatt ttttctatat ttatttttct
    35401 gtttttttat ttaacatttc aataacagtt cgaagatctt ttccttccgc aagaagtttc
    35461 aatttttcct tttcttcgac ggtataattt cggacaaaac tggaatcagg tatcaaaaca
    35521 acgcctaatc ctgctcgtga agcagctgtt gctcctacta aagaatcttc cactgcaaag
    35581 gctgaactag cttctaatcc tgttcgttcc aaagctgtca gataaggaat cggtgaaggc
    35641 ttgttctccg acacttcgtc tccgaatgta aacgtgtcca agtaatcagc taatttaaag
    35701 tgatccaaga tatctgttgc tcttttcgta acagtggtcg tagcaatgcc tagtttcata
    35761 tgacgttctt ttaagtctgc caatatttct tttgcatatg gttttaagtg gatggctccg
    35821 cttgtcagct gttgataaaa atattcttca cgcttcgctc ggacttcctg tactttttca
    35881 gggctacctg ctgctttcac taaaatatca aaactttgtt tccagttttt tccgccccaa
    35941 gcgtcaacga tttcttttgg gatatggata tcaaattgct gaaatcccca aagccagcca
    36001 tcacgatacg ttttttctgt gtcaactaac aaaccatcta aatcaaatat tgcacctttc
    36061 atctatacat taatcctttc caaaacaata cacatagttt agcacgactg gtccaaaaag
    36121 aacagaagca aagaaccagc cttctcgttt ataagagttc ttggaatcga cggatataga
    36181 ttttcttcac agctgtcaca agcgccagat aagcgatgac tgtagcaaat aaccataacc
    36241 agtaattgcc aggaagtggc atcaagccta attcagcacc aaaacttgta aaaggaagga
    36301 cagaaccgac aataattcct aatgtagtga taacaaacat ggcaaatgtt gcgttgcttt
    36361 ggataaatgg tactttcggt gtacgcagtg catggagcac taaggtttgt gtccatagtg
    36421 attcgacaaa ccatcctgca tggaataatg cgatgaaaat cactcgctgt gaactatcca
    36481 atgtatggaa acttccgccc actacagcag gacaaataac aaaatacatc aacaagtaag
    36541 tagtaatatc aaatatcgaa cttgttggac caaagtaaac catgaattcc ccaatactag
    36601 atgcattcca tttttttggc tcgtccaaat attctttatc catccgatcc catggcaagg
    36661 aaatgcagga aacatcatag atcagattca aaaacaaaag ttgtaaaggc agcattggta
    36721 aaaatggcag gaacgtactt gcaatcagca cggaaaacat attgccaaaa ttagaacttg
    36781 ctgtagcctt gatatatttc atgatattcc caaaagtttc tcgtccagaa agaaggcctc
    36841 tttccaaaat cgtcaaatct ttttctaaaa ggatcacgtc tgcagattct ttcgcaatat
    36901 ccacagctgt atctacagaa ataccgatat cagccacttt cattgcagga gcgtcattga
    36961 taccatctcc taaaaatccg acagtatgac catttttacg taaagctgtc gtcaaacgtg
    37021 ctttttgctg aggattcaat tttacgaata cttcatggtc ttcagctact tgtgctaatt
    37081 catgatcact catttccgag attttctcac cggtaaccaa ttcattgatc tctagaccta
    37141 cttgacggca cactgaacgt gtaactaatt cgttgtcccc agtcagaact tttacagcta
    37201 caccatgttc tttcagagct ttcaacgctt gtttcgttgt ttcttttggt ggatcaagga
    37261 aagcaagata accaatcaaa accatgtctg actcatcttt gactgaaaat tctccaacga
    37321 cgcttggatt ggtcttttgt gcgacaccaa tcactcgtaa gccatcttca ttaagtgact
    37381 gtacttttgc aagtacggct tcttttcgtt cttttgttaa tggtgtaaca gtaccgccaa
    37441 gatcaataaa tttagaaatg ttcagcattt cttcaatcgc gccttttgtg atcatttgtg
    37501 ttttaccagc tggatcagca accacaacac tcatacgacg acgttcaaaa tcaaaaggaa
    37561 tttcatccac tttttgataa ttgatcttat ctgtatctaa agtttctgtt gcttcatcaa
    37621 tgatagccac atctaacaaa tttttcagcc ctgtttgata gtagctattt agatacgcat
    37681 ggcgcaaaac acgatcatct tcatttcctt cacaatccat atgatattca agaatgattt
    37741 tatcttgtgt caatgtcccc gttttatccg tacataacac atcgattgca ccaaagttct
    37801 ggatggcatt caaatttttg atcactgttc cttttttggc catcacactt gctcctttga
    37861 ccaaattcgt cgtaacgatc attggaagca tttcaggtgt tagaccaact gctacagata
    37921 gtccaaaaag aaaagcttct agccaatccc cttttgtaag cccattgatg acgataactg
    37981 ctggagccat caatgacata aatttgatca gtagcatgga tgttttatgg atgcctaatt
    38041 caaagcttga ttgaattggt ttttcagata gcgtttctgc tacatgacca aatagtgttt
    38101 catttccaac agaaacaatg attccttccg cacttccact gatgacatta cttcccataa
    38161 agacaagatt ttcgtaactt gtttcgcttt gctgatcgtc ttcacatact ttcgcatgct
    38221 tttctactgg atagctttcc cctgtcaatg ctgcttggga aatgaataaa tcctttgcgg
    38281 acaataaacg catatccgct ggtatcatat cccctgctga aagccgaaca agatctccac
    38341 agacgatttc ttccatagga atctctgtgt actttccatt acgttttaca gctgccgtta
    38401 ctttcacaag tgatttcagt ttttcagccg cttgattgga acgtacggac tgaatcaacg
    38461 tcattgtccc gctgacgata accatagtga agataatggc tacaccgaaa agatctttat
    38521 ctccaggagc tgctaataca tattccgtga tgaatgaaat aattcctaat gcgatcaaca
    38581 ctaatgtaaa tggcgtgatg tatgctttca atatttctac aatgaatggt gtttttttgc
    38641 catatgaaat cgcattttca ccgtaatttt ctcttgaacg ttcgatttgt tcttctgtca
    38701 atccatttgc tgatgcatgg aatagttcaa ataattcttg tttcgttttt ccagcaaaag
    38761 ctgcatagat tttttgtgta tctttctttt tttccatctc aatctctcct ttgtgtccaa
    38821 aagacagctg ttctcgctgc ttttggtctt tagctattca tcggatcgat agagaaaaaa
    38881 ctgaaagggg ctgagaacag atagataata gccctggcct ttcctctaga tccttgtttt
    38941 tcgaatgact gtcatccatt tgttctcatc tcctttacta agtaatttta attcatgact
    39001 tccaatcatt tttcgaatag ttatttttcc aattagacca cctccttttt atttttcacc
    39061 gatggggatt tgcggcaaca aaaaaacctc tcgccaaata ctcagcgaaa ggtttgaaaa
    39121 agcatattaa aaaaagcttc tcagatctca tcgttgagtt ttgacactgt acaacgtaaa
    39181 aagcagatac ctgcttttag ctaccttatg aaaagcctta tctcgacaat tcctgttcta
    39241 cccattggcg tctctcgacg tttttgggca gtagcctatg tttgtatagg agcctcacct
    39301 aacaaatctt attcggtttt ccttcgttaa tataacaact aaaaaagagg atgtcaaatc
    39361 aattttccat gtttttcata ctttttttaa aatacacacg ttatattcct gtctcacgcg
    39421 gttttatttg tctcattttc cacctcccgc tataatagaa atatagtagg aaacgaggtg
    39481 ctgttatgga aagaacactt gttattatca agccagacgg cgtgagacgc catttagtcg
    39541 gaagcatcat ccaacgtttt gaagcaaagg gactggccat tgctgaaatg aagtttgaaa
    39601 ctatgactcc tgaattagca aaagaacatt atcagcactt gactgagcgc agtttttttg
    39661 atgaactgat cgattacatg acttccggtc ctgtcgttta tcttgtgctg gtcggcgagg
    39721 aagtgatcga tatcgtccgt aagatggttg gagcaacaaa agctgccgat gctgttccag
    39781 gtacgatccg aggagactac gcgctgccgg ggactgaaaa catcattcac gcttctgatt
    39841 cccgtgatgc tgcggtaaaa gagatcgccc gctttttccc gccattcgat ataaacaaac
    39901 cgtctgaaaa agcggcgaag tgatacaaat tctaaactgc tggtctagtt gctgggttca
    39961 gcaatggcca gcagtttttt ctctaaggac ctccgttgat tctttcctaa catatattcg
    40021 agcactgtga agaattcttg gtagtctttc gtcttttctt cccctaaagc agcagaaatc
    40081 aatctttgat aataatctgc catcaatgtt tcatagatca ctgtacttct ttctttatgt
    40141 gctaagctcc gtttcatatc ttcattcaca ctacgtgcct ttttcaaccg accattttct
    40201 atataaaacg aagtcaggtt cattaaaaaa gtaaagagat actgctcatg attgttcaac
    40261 tgtgctaatc gtcgcattct ttttttgatt ttcaaataat tgttctcaat gaaccgatca
    40321 ctgaatatcc aaggaaaatc agctagaata gatagttcaa attgaccata gtctggtatc
    40381 tgcatcaagt attcttcaat gattcggcat tcataacgta aaaattcaaa ttctgtctca
    40441 gatacttctg ttttttcgac catgtgaaga ttcaaaacca aataatacag atcttctgtt
    40501 ttcttaaaat gcctacggtt attgctgatc tctttataag gatcaaagct gtcttcttgt
    40561 acccgatcta gcattcgtct ttttttcttt aactttattg gttcatccag ctggtttcgc
    40621 atccattcat attctgcaag tgtcatgttc attcgctcta aaaatgatct caatgttaca
    40681 aatgacattt ttttcactga atttgtctca atcccgacta gagaagtctt aaaacagatt
    40741 ccttccgcta attgggactg tgtcaacttc ttgttgactc gaagtttctt gatcaattca
    40801 ccatcataca taatagttct cctttttaat ttaaattttt ccacattcaa taaatagata
    40861 cgttaaaaag gaaaaataca cgaaaaaaat aaaaaaatgt ttttttcata aaaaatacca
    40921 accttcaagc attagaatgt tgtctaacta ctgaaggttg gtttgattgt ttattgatta
    40981 tttttttgct gttcaacgtc tgttcttgtt actggatata agttggttgt tgttttatta
    41041 ccattttttg aaaagacact tgtcgaatgt tcgccttttt gcttttcgat tgcttcatta
    41101 cgttccgctt catttttata gtcatatttt ttcggatcaa tcgtctcaaa tccgtttggc
    41161 acataaaacc gcaagagatt ttcctgattc agcttatctg aaagacttaa cgcagtcttg
    41221 actttttcct gatctttgga gatttcctca tcgattcctg ctgctttcgt atcgatgatt
    41281 tctcctgtct gattattgta agcttttcct cctaggacgg tgtatttggg tgtcacaaag
    41341 ttcccatttc gaaaagcaac tacttgatca tgctgagaag ataataagtc tgtaccaaaa
    41401 tgaagataat ctttatcatc gattcccaat agatgtagta atgtcggcaa aacatcgatt
    41461 tctccaccgt attcatgatt gactgtccct ttagtataac caggcatatg gatcatcaat
    41521 ggcactcgtt gcatctgtgc gttgtcaaat tcatcccatg tatctggatc tttaccaaga
    41581 gcgcttgcta aatctttatt atctgtattc gagatcccaa aatgatcccc gtaaatcaca
    41641 aagattgaat tttggtaaat accagatttt ttcaaatacg taaaaaattg ttccaatgat
    41701 tgatccagat aatgtgccgt tcgaacataa ttatctactg tgctattgcc agtattcaaa
    41761 gatggaaagt cgaaattctt gtcatccgta taataaggcg tgtggttggt tacggaaaga
    41821 aatttcgtgt aaaacggctg ttgtagatgc tcaagatatt gggcagattc tctgaataaa
    41881 tctttatcta atatcccata acctaacgtt tcgtcggatt catcaaaata agatcgatca
    41941 aagaaattgt cataacctaa gtttttatat acatgatctc tattccaaaa actagctaca
    42001 ttcccatgga aaacagcgct tgtatagcct tgcttttgtc caaggatcgc tggtgcggct
    42061 tgaaagacat tgtctgaccc aagttctgtg aataatgagc cttgaggtaa accaaatgtg
    42121 ccagtttcca acatattttc agcatcactt gtctttccct gtcctacttc atgaaagaaa
    42181 ttgtcaaagc tgatcgttgc ttgattctgg aaaatactat ttaaaaacgg tgtgacttct
    42241 tgtccgtcta ccttcatatt aatcaaaaac tgctggaaac tttctaaatg gagaacgatc
    42301 acatttttcc ctttagcgat cccaaatgtt tcgggatttg gctctgcata atgtttcttt
    42361 gtataatcta aaacttcatc aatcccatta ctgcttgcat gcgcgcgaac actgctagtc
    42421 attcctgttt tgattccgtc ataaatagta tacgcatcga ttcctagata tttgacaata
    42481 tatgaacggt caaatgttcg ctgcagcaat tgtggacggt tggcttcgct cagcgctaga
    42541 tttcctgaaa aaataagccc tgaaagacag gtaacagcga tagccatcgg tttgccgaca
    42601 gggttgcttt tgataggtat tttcttgacc ttcaaccaaa ccagtagccc aatgaacaca
    42661 gcgatatcca gccagtagat gacatcatgg ggcttcatca aagaaaagga gcttcctgac
    42721 aatccttgcg acactttcga aaagcctaat acagatttga ctgtaatgaa atctgtgaat
    42781 tcccgataaa aaataatatt caagtacaac aaaagcgtat ttaaaatatc aatgatcaaa
    42841 agaatagcta acgctggctt tggcttttta atatacaaag aaaggccaaa aaacaataag
    42901 gtagtcgcaa ttgggttgat ccacaagata aagtactgga tcgtaccttc cacacctaat
    42961 gaaaaatctg catagtaggc aatgatcgtc ttcagccaga aaaagaaaac agctgtcaaa
    43021 acgattgcta aacgattttg gcacaatttt ttaagttgtg tcacgtatat cacttctctc
    43081 tattcacatt ttttcttatt ttgctcctga aaaatgaaaa tttcctaaaa aagttgttat
    43141 cttaagattt ttttattctt tctaaagatt attccgtcca cttttcacag cataaaaccg
    43201 ctgcgatgta agcgattaaa ctaatgaaga gataaaagtc ttgtggtgac cgcttcactc
    43261 cacggtattc aaaagccagt ttctcggtat tcgtacaaaa aaagaggctg tgacaaaatt
    43321 cttgtcgcag ctccttattt cttgatgcag gacagctttt tcacaacttc ttccttaaac
    43381 ggtgttctcc tgccaactcc tcggccacaa gtcacaatat ttataaaaca tgagaagctg
    43441 ttttttgaaa ttgttccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac
    43501 acggtgttca atcagctgac cctcggtatt agattatgtc aggacgctaa ttttacaacc
    43561 tcttggtaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
    43621 tataaggagc tattttttga aattgctcct tgtggctcag agttaaacgg caggctcaag
    43681 acctcttgat a
//
LOCUS       DACEUF010000013        43052 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.014, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000013 DACEUF010000000
VERSION     DACEUF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.014"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            211..1080
                     /locus_tag="IXG20_000705"
     CDS             211..1080
                     /locus_tag="IXG20_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1608921.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIISSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1289..2314
                     /locus_tag="IXG20_000706"
     CDS             1289..2314
                     /locus_tag="IXG20_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1608922.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2353..3303
                     /gene="manA"
                     /locus_tag="IXG20_000707"
     CDS             2353..3303
                     /gene="manA"
                     /locus_tag="IXG20_000707"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ1608923.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3397..5100
                     /locus_tag="IXG20_000708"
     CDS             3397..5100
                     /locus_tag="IXG20_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1608924.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSIIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5102..7135
                     /locus_tag="IXG20_000709"
     CDS             5102..7135
                     /locus_tag="IXG20_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1608925.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRCVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7146..7586
                     /locus_tag="IXG20_000710"
     CDS             7146..7586
                     /locus_tag="IXG20_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1608926.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7786..8913)
                     /locus_tag="IXG20_000711"
     CDS             complement(7786..8913)
                     /locus_tag="IXG20_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1608927.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8916..9593)
                     /locus_tag="IXG20_000712"
     CDS             complement(8916..9593)
                     /locus_tag="IXG20_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1608928.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9831..11624
                     /locus_tag="IXG20_000713"
     CDS             9831..11624
                     /locus_tag="IXG20_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ1608929.1"
                     /translation="MKRREGETKWNGRFPKNNISLKARVQKLGSTLSSMVMPNIGALI
                     AWGVLTALFIPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATM
                     GVIVGTDIPMMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLAL
                     LGFSAIGPVVDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLG
                     TEQVAEAGKSILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYF
                     PYVMMKPLLFLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILG
                     VIVAAAVSFGISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQK
                     IIFACDAGMGSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERA
                     KQKAPSARFVSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEI
                     LLVHDDRSGSATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQ
                     MAAPQSIHLSVNSMVNPQKYESIAQYLKQTA"
     gene            11639..12076
                     /locus_tag="IXG20_000714"
     CDS             11639..12076
                     /locus_tag="IXG20_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1608930.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12089..13240
                     /locus_tag="IXG20_000715"
     CDS             12089..13240
                     /locus_tag="IXG20_000715"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ1608931.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13302..14000)
                     /locus_tag="IXG20_000716"
     CDS             complement(13302..14000)
                     /locus_tag="IXG20_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ1608932.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13993..15345)
                     /locus_tag="IXG20_000717"
     CDS             complement(13993..15345)
                     /locus_tag="IXG20_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ1608933.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15568..16968)
                     /locus_tag="IXG20_000718"
     CDS             complement(15568..16968)
                     /locus_tag="IXG20_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ1608934.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17129..17866
                     /locus_tag="IXG20_000719"
     CDS             17129..17866
                     /locus_tag="IXG20_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ1608935.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17991..18470
                     /locus_tag="IXG20_000720"
     CDS             17991..18470
                     /locus_tag="IXG20_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ1608936.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18467..19234
                     /locus_tag="IXG20_000721"
     CDS             18467..19234
                     /locus_tag="IXG20_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608937.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19464..20033
                     /gene="lepB"
                     /locus_tag="IXG20_000722"
     CDS             19464..20033
                     /gene="lepB"
                     /locus_tag="IXG20_000722"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1608938.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20137..20739)
                     /locus_tag="IXG20_000723"
     CDS             complement(20137..20739)
                     /locus_tag="IXG20_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1608939.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20845..21314
                     /locus_tag="IXG20_000724"
                     /pseudo
     CDS             20845..21314
                     /locus_tag="IXG20_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21420..22004
                     /locus_tag="IXG20_000725"
     CDS             21420..22004
                     /locus_tag="IXG20_000725"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ1608940.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22007..23080
                     /gene="prfA"
                     /locus_tag="IXG20_000726"
     CDS             22007..23080
                     /gene="prfA"
                     /locus_tag="IXG20_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ1608941.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23073..23912
                     /gene="prmC"
                     /locus_tag="IXG20_000727"
     CDS             23073..23912
                     /gene="prmC"
                     /locus_tag="IXG20_000727"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ1608942.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23905..24084
                     /locus_tag="IXG20_000728"
     CDS             23905..24084
                     /locus_tag="IXG20_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608943.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24106..25131
                     /locus_tag="IXG20_000729"
     CDS             24106..25131
                     /locus_tag="IXG20_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ1608944.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25306..26550
                     /locus_tag="IXG20_000730"
     CDS             25306..26550
                     /locus_tag="IXG20_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ1608945.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26703..27650
                     /locus_tag="IXG20_000731"
     CDS             26703..27650
                     /locus_tag="IXG20_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1608946.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27784..28413
                     /gene="upp"
                     /locus_tag="IXG20_000732"
     CDS             27784..28413
                     /gene="upp"
                     /locus_tag="IXG20_000732"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ1608947.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28552..28794)
                     /locus_tag="IXG20_000733"
     CDS             complement(28552..28794)
                     /locus_tag="IXG20_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608948.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28915..28987
                     /locus_tag="IXG20_000734"
     tRNA            28915..28987
                     /locus_tag="IXG20_000734"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28948..28950,aa:Thr,seq:cgt)
     gene            complement(29106..29744)
                     /locus_tag="IXG20_000735"
     CDS             complement(29106..29744)
                     /locus_tag="IXG20_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1608949.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29867..30499)
                     /locus_tag="IXG20_000736"
     CDS             complement(29867..30499)
                     /locus_tag="IXG20_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1608950.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30850..31149
                     /locus_tag="IXG20_000737"
     CDS             30850..31149
                     /locus_tag="IXG20_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1608951.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            31644..33809
                     /gene="acm"
                     /locus_tag="IXG20_000738"
     CDS             31644..33809
                     /gene="acm"
                     /locus_tag="IXG20_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ1608952.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(34081..34566)
                     /locus_tag="IXG20_000739"
     CDS             complement(34081..34566)
                     /locus_tag="IXG20_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ1608953.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            35046..35687
                     /locus_tag="IXG20_000740"
     CDS             35046..35687
                     /locus_tag="IXG20_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1608954.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            35706..36341
                     /locus_tag="IXG20_000741"
     CDS             35706..36341
                     /locus_tag="IXG20_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1608955.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            36359..37174
                     /locus_tag="IXG20_000742"
     CDS             36359..37174
                     /locus_tag="IXG20_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1608956.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            37493..38929
                     /locus_tag="IXG20_000743"
     CDS             37493..38929
                     /locus_tag="IXG20_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1608957.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            39118..41565
                     /locus_tag="IXG20_000744"
     CDS             39118..41565
                     /locus_tag="IXG20_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1608958.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            41795..42862
                     /gene="rlmN"
                     /locus_tag="IXG20_000745"
     CDS             41795..42862
                     /gene="rlmN"
                     /locus_tag="IXG20_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1608959.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
BASE COUNT        14781 a         7362 c         8816 g        12093 t
ORIGIN      
        1 gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc
       61 tctttttata agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct
      121 cacatttttt ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa
      181 taaagcttga cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc
      241 tatttaaaaa gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag
      301 aacgttgttg gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa
      361 gaagctggaa gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg
      421 gtgatcaaaa agctgaaccg tttttcattg attatctcta gcacagaaga aatggcagat
      481 atcctttgtc gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga
      541 cgtatgggga ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag
      601 aataagaacg accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa
      661 tctgaagaaa aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat
      721 gttactaact taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat
      781 aatgaagaag agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa
      841 gctagcggag gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa
      901 ttcatgatcg atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt
      961 gatttcttct tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa
     1021 tggtggttcg tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa
     1081 ctttttcttt cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc
     1141 ttattctaga gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc
     1201 agtttttttg tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa
     1261 gaatctgata gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa
     1321 aaagatggaa gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt
     1381 agaaacattt acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga
     1441 tctgttgttt gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg
     1501 tctattagaa gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt
     1561 tgaaaatcag acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg
     1621 gataccaaac agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga
     1681 accaggtgag caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg
     1741 gttgatgcta gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt
     1801 ggaaccatta ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc
     1861 cacagaacaa ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag
     1921 agttttggct gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac
     1981 ttttgtccaa atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga
     2041 gacgattgat tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg
     2101 atgggactta aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac
     2161 aggtacgttt ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg
     2221 cgtacatcct gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa
     2281 ctttttgaaa gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga
     2341 ggagtttgaa ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg
     2401 ggaggaagtc gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa
     2461 gattgggcga tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa
     2521 gggaaaagat tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa
     2581 ccagtgtttc cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta
     2641 catccagatg atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg
     2701 tatatcatcg atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga
     2761 gaagaactag ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca
     2821 gttaaaaaag gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga
     2881 atcatgattt tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac
     2941 agaaaagatg cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca
     3001 actgtccctg cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg
     3061 atcgtcactt atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc
     3121 acatctttca aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac
     3181 cttgtcgttg acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat
     3241 ggcgtttctg agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa
     3301 tagttcccag atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa
     3361 atccgtccat caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac
     3421 aacagcagtc gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg
     3481 atcatgccaa atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt
     3541 gtgtctggcg atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt
     3601 ttacccatct tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc
     3661 gccggtgcaa ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga
     3721 gcgatgatta tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat
     3781 gtgaaattca aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt
     3841 agcgttattt gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg
     3901 ttgctcgcct ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg
     3961 atcttagaac ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca
     4021 cctttaggaa tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca
     4081 aatcctggac ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa
     4141 aaagtaaatg cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat
     4201 tttccatttg tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc
     4261 ggcacgttga tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca
     4321 ctactcgtga tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata
     4381 gcaatcagta catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa
     4441 gaaaaagaga acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat
     4501 tccatcattt ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg
     4561 aaaaagaaat tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa
     4621 ttgaataacc aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga
     4681 gaaaaagtac ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg
     4741 ctgtcacgaa taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca
     4801 gatatcacag aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga
     4861 cgcggctcac aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa
     4921 cagtttgtta tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat
     4981 atcgcacaaa gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt
     5041 ctagttgaac attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa
     5101 gatgtattta tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc
     5161 tgtatctatc caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga
     5221 attagataat cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag
     5281 aggctcattt gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga
     5341 tgggaaaagt aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt
     5401 actgacgaca gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac
     5461 cactttttat gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat
     5521 cattcggaac cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc
     5581 gaatgtatta gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc
     5641 atcactgaat tatttttttc gatgcgttga tccgcaacat ctatctcttg cacgcaaagt
     5701 aatcggggaa gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt
     5761 ggttttgatg ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga
     5821 gacatataat agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg
     5881 gatcgcttct gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt
     5941 attaaatgac ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca
     6001 gcttgcttat ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt
     6061 ttatgaagat gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag
     6121 agccgtttta caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata
     6181 cgaggaagtt gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt
     6241 atccgaagaa gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc
     6301 gaaagaaagc aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat
     6361 gttggaacta cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat
     6421 tgctgattta aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat
     6481 tttaccagga tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt
     6541 caagcagcta aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta
     6601 taacagcaat aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga
     6661 tcaaatcaat caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat
     6721 ttccgaaacg attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga
     6781 ggtggttcat cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac
     6841 aaatatgggg ttattccata catccagctc attgatttct aaaccgatct tttgtatttt
     6901 caatttgaaa catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg
     6961 tatgttagtt atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa
     7021 gatcagtgga gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa
     7081 agaaatcgtt tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg
     7141 aaaagatgat acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga
     7201 aagaagcgtt gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat
     7261 atatccaatc aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag
     7321 ccttgccaca tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc
     7381 aagtgcctga tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat
     7441 ttgtagtggc tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct
     7501 gttcagacat cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa
     7561 tgatttttga acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc
     7621 aagtaataac ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat
     7681 tactgcggat gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg
     7741 ggacggtgac aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt
     7801 tatatagata gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa
     7861 gataccattc atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat
     7921 gatcacattt tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca
     7981 tccaatcgaa gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag
     8041 attgactaga gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag
     8101 cacccacaac aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg
     8161 tatctcctga ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat
     8221 tattactagt atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata
     8281 ataaatggta aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag
     8341 agagattacc tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat
     8401 acataaaatc aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa
     8461 agagagatgc gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata
     8521 acggagcaac tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga
     8581 aatcagtcga tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac
     8641 attccctgtg ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact
     8701 ttccacaaat tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc
     8761 ttcatagtta tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag
     8821 tgggaatgca aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag
     8881 taagagattg cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa
     8941 atagctaaaa tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa
     9001 catgctttag aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc
     9061 ttcccctctt tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct
     9121 aatccgttca acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca
     9181 agcaagacat gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca
     9241 gtcatcttca atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg
     9301 atcaaagtca aataatcaac agccgataga tagtcggata actgattcgt tgattctaga
     9361 aaaaagaaag accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat
     9421 tgatcataag ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat
     9481 ccgttcgggg caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga
     9541 atcacacgtt ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg
     9601 cctccaatat aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca
     9661 aaagttattg tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt
     9721 tttctgatac cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc
     9781 gtgccaaagt acgactattt ctctttttag taagcggata caattagagt atcaaaagga
     9841 gagagggaga gacaaaatgg aacggacgat tcccaaaaaa caacatttcg ctaaaagcga
     9901 gggtacaaaa attaggcagt actttatcaa gcatggtcat gccaaatatt ggagcactga
     9961 ttgcctgggg tgtattaacg gctctattta tcccagatgg ttatttacca aatgaggctt
    10021 tcgctaccat ggtaagccca atgctaactt atctgattcc tttattgatc ggttatactg
    10081 gcggaaaagt aattgctggt gaccgcggat cagttgttgg ggcaattgca acgatgggtg
    10141 tgattgtcgg aacagatatt ccaatgatgc taggcgcgat gatcatggga cctcttggtg
    10201 gatatgccat caagaaattt gatcagctat tccaaaaacg gatcaaatcc ggttttgaaa
    10261 tgctagtcaa taatttttca gcaggtctta tcggttttgg attagcgtta ttaggttttt
    10321 ctgcgattgg tccagttgtc gatgcattga ctcaagcaat ggcaaaaggt gtagagatca
    10381 ttttatctgc ccatttgatt ccattgacaa gtatttttat tgaaccagca aaaatcttat
    10441 ttttgaataa tgcaatcaat catgggattt tgacaccatt gggaactgaa caagtagcag
    10501 aggctggaaa atctattcta ttcttgcttg aagcaaatcc tggtccagga cttggcgtat
    10561 tacttgcttt tactttgttt ggcaaaggag cagcaaaatc ttcagcacca ggagccatga
    10621 tcattcactt tttaggcggg atccatgaaa tctattttcc ttacgttatg atgaaaccgt
    10681 tattattctt atcagttata ggcggaggaa tgtcaggaag tttcattttc caattgatgg
    10741 atgctggact aagagcgcca gcttctccag ggtcgatcat tgcgattcta gcgatgacac
    10801 cgttcaattc ttaccttcct gttattttgg gcgtgattgt tgccgcagct gtttcgtttg
    10861 gtatttctgc agtaattttg aaagcggatg cgaaagatac aaatgaagac ttcgaaaaat
    10921 ctgttcaaga gacaaaggca gctaagcaag cttccaaagg tgcccaaggt attcctcaag
    10981 gagctacatt ctctggcgta caaaaaatca tttttgcttg tgatgcagga atgggatcaa
    11041 gtgcgatggg cgcttcgatc ttgagaaaaa aagtacagga agcagacttg cctcaaacgg
    11101 tgaccaatca agcaatcagc caactgacag atgactcgaa tacattgatc gttactcagg
    11161 tcgaactaca agaaagagca aaacaaaaag caccgagcgc gcgttttgtc tcagtagaaa
    11221 actttttgaa ttcacctcga tatgatgaaa tcattcaaga attaagcagc aagcagaaaa
    11281 catcagtttc tgaaattgag aatgagccag cagaagagtt tctttcagca aacatcaaac
    11341 aaatcaaaga gattctactt gtccacgatg atcgttccgg atcagcaaca atggggatga
    11401 ccgtgctcaa agatatatta gacaagaacc atttaactat cccgttaaga aaagtcaata
    11461 tccaagattt aaaagatgaa cctgattcac tgatcgtagc caaagaaagt ttagcggaaa
    11521 aagtacagat ggctgcacct caatccattc atttatcagt caacagcatg gtcaatccgc
    11581 aaaaatacga atcgattgca caatatttga aacagacagc ttaagggagg aaaacagcat
    11641 gcaaagtttg ccattcgaca tgatcgagct caatcgatct tttaaaacca aagaagaagc
    11701 gatccgctat tgcggtcaga tgcttgtaga agcagggtgc gttgatgagg aatatatcga
    11761 tgccatggta gatagagaca aaatgttatc tgtttatatg gggaatttta tcgcgattcc
    11821 tcatggaaca gatgaagcaa aaaaatatgt gaaaaaatcc ggcatctgtg tcgttcaagt
    11881 accagatggt gtagattttg gtaccgaaga agaagaaaag attgcgactg ttttgttcgg
    11941 tattgcagga gtcggagatg agcatttgca acttgtacag caaatcgcac tttattgcag
    12001 tgatatggac aatgttgtcc agctagcaga cgcgctcagc aaagaagaag taacggaaaa
    12061 tctagcaatt gcttaaggag agagaaaaat gagagcagta cattttggtg caggaaatat
    12121 cggtcgagga tttattggag aggttttggc agctaatgga tttgagatta ctttcgtaga
    12181 tgtgaatcac acgatcattg atgctttaaa tgaaagaaaa gaatacgaaa tcgaattagc
    12241 agatgaatca aaagaaaaaa tcactgtaaa aaatgtcaaa ggaatcaaca atcaaacaaa
    12301 tccagaaaaa gtaatccaag aaatcgctga agcagattta gttactaccg ccattggccc
    12361 gaatatctta ccttttattg ctgagttgat tgcaaaaggt atccaagaaa gagaaaagga
    12421 aggaagcaca acatctttag atatcattgc ctgtgaaaat atgattggcg gttcagcatt
    12481 tctagaaaaa gaagtgtata aatatcttcc ggaaacatca tttactgaca aatatatcgg
    12541 ctttccaaat gcagcagtcg atcgaatcgt cccattacaa caccatgaag atcctttatt
    12601 cgtccaagtg gaacctttca aagaatgggt cattgacgat agccaaagaa aaaataaaaa
    12661 aatccaatta aaaggggttt tatatgtaga tgatctagaa ccatatatcg aacgcaaact
    12721 atttagtgtc aatactggtc atgcgactgt cgcttataca ggtgccttgt taggttacca
    12781 gaccattgat gaagcaatgc aagatgcgtt agttgtggca caattaaaat cagttttgca
    12841 ggaaacagga agtttgctga ttgccaaatg ggggtttgat gcagagcagc atcatgcgta
    12901 tattgaaaaa atcattcatc gcttccaaaa caaaaatatc tctgatgcga tcacacgtgt
    12961 cgctcggaca ccattacgta agttaggtta tcaagaaaga tttacacgtc cagtgagaga
    13021 actacaagaa cataacttga cttgcccaca tttgacggca acaatgggga ttatttttaa
    13081 ttactatgat cctgaagatg aacaaagccg tcaattgcat gaaatgaaga tccatgaaaa
    13141 cttggaacaa ttgattcaag aagtcacagg aatcaatgat ccgaaaacaa tcgggaatat
    13201 caagcaaaac gtgaaccgtt atgccaagca agtcgcttaa aataggatac gaaaaagggg
    13261 aatgtgacga aatttttgtc acattcccct ttttcgaata actaggctgt ttgtccttca
    13321 agatagggca gatccaattc tttaccgtaa cgttgttcta aagctaggcg gatcaaacga
    13381 gctgccaggt tctcatttct tgcaaggata ggtccatgga aataagaacc gaagacattt
    13441 cggtaaatga caccttcaga ttggtcttca ccattatttc cttgaccttg taccactttc
    13501 cccaaaggtc gttcaccttc acctaagaaa gtccgaccat tgtggttttc aaatccataa
    13561 taagtttcat tgaattcttc gttatggata acgatatcac cgataaaccg gttattttcc
    13621 tgacttaacg tgtagtgatc cagtgcactg attccttgga tcttttctcc attagctccc
    13681 atgtagtagt gacctagcaa ttggtagcca ccacaaatgg ctaacatgac gccatcattt
    13741 tcgatatagt tgatcaaaga atctttcttt ttctggatgt cttttgaaat gattaattgc
    13801 tcaaagtctt gaccaccgcc aaagaaaacc aagtcatatt tatcagcatc aaaaggttca
    13861 tgtatactaa cgatctccgt atggcaagaa acccccattt tttctgctat atatttcaac
    13921 atcaaaaggt ttccgttatc accatatgtg ttcatcaaat ttccataaag gtgggcgata
    13981 ttgagttcat aattagccac ggttcattcc tcctttgata aatccttgag cagctagttc
    14041 tttacgaagc tgcaatacag cagtgtaagt agctaaaata tagacatgct cagttggcaa
    14101 ttctttgatt ttttcgatta cttctggtaa ttcttttgtt tctgtcaatt tattttctgg
    14161 aatcccggca acttttagac gtaaagtcat atctgtatga cggtctcctc cagaaataac
    14221 agctgggata tccatatcgg caaagttttc aaaattacca tcccaaatcc agctgacatc
    14281 gattccgtct gcgtaattgg catttaataa agccactaaa gaaaatggat aaggagctag
    14341 accaatcata tcgattactt gattgatacc tacaggattt ttgacaagta ctaatgtaca
    14401 ttttttccca ttgatattga tgacttcttg tcggccgaat actttctcgt catatcctaa
    14461 accttgtttg attttatcag gatcgacatt gaagtactct gccactgcag tagctgctaa
    14521 agcattgtag acattataca taccgccaac ttcgattccg tattcatgac catcgatcac
    14581 gaaatctgct gacacattgt ccatccgtac catatcagtt aatcgataat ccaattcagg
    14641 acgtttgaag tcacaatttg gacagtaata tttccctaag tttgcataag tgatcatttt
    14701 ataatgcaag atgtgatgac aatttggaca taagacaccg tctgtattgt aatgagccat
    14761 ttgttcacga tcttcttcat ggtcaaagcc ataatatttt ctaggattga ctgtttcgat
    14821 agaattgaag attggtgaat caccgttact gataatcgtt gcatttggtg actttgcggc
    14881 accgtctaca atcattttat aggtcgtata gatttctcca taccgatcca tttgatcacg
    14941 gaaaatattt gtaaataaga acagttctgg tttcatatat tctgtgactt tgctcaaact
    15001 tgcttcatct atttctagga cagcaaactt tttctgtcct ttttttggtt ttgcctgcaa
    15061 gaaagtcgat acaattccct gtaccatatt cgcaccagta gggttggtta gaacttcatc
    15121 gaattgctgg cgcaaaatat tgactgttaa agcggtagtc aatgttttac cgtttgttcc
    15181 ggtgacaacg acgacttggt agtcttttgc caaactatct agtatatgtg gatcgacttt
    15241 cagcgctagt ttacctggta aacttgatcc gcctttaaaa aaagttttta atgcccattg
    15301 cgacgttttt ccaactgcaa tcgcaaaatg actgcgtatt cccattatgt aacctccaaa
    15361 aacgatttgc tgacgaataa attcaactaa tcataccata tgttgaaaaa aaggttaaat
    15421 tgcttttctt attcttaaga aaaggttacg gctgaaagtg catgaaatcg gacaaaatcg
    15481 catgatttaa caaaaaaaca gcttgaaaca aaatgattgt ttcaagctgc tgtgatttta
    15541 ttattccctc ttacctatat tcccatttta accgataata attttctcgc ttggatattc
    15601 atagcctaga tctcgtgttt cttttggcac gaataacatc aatgtatata gcattccgat
    15661 ccgcccaata aacatcagtg tagcaatcgt gattttgccc acaacagaca agtctccggt
    15721 aatccctaaa gataaaccag ttgttccaaa cgcagaagtt acttcgatga tgatcgagat
    15781 cagcgtttgt tcttctgtag cagataaaaa aacgatacaa aagaaacaca taccaagaga
    15841 aagcataaag acaacgactg attttcgcac atcatcttga tcgattctcc gtccaaaaat
    15901 attgatgtta tcttcacttt tgaggaagga atatagatac aaaccgatga ttgctacagt
    15961 agttgttcga atcccgccgc ctacagaact aggactgcat ccgataaaca tcagtaaaga
    16021 aaaaatgatc agtgttgtga tttgaaaatc acccaaatcg tgaatctgca gaccagcatt
    16081 acgagtcgta atcgaataaa acatcgaatt gatccatttg acactcatat tggaatcttg
    16141 gaataaatgg tccttttcca atagataaat caagacagtt cccgaaacaa acaagataac
    16201 aaaagctaac acagctaatt tggtaaacaa ggaaaaacga aacggaagct tcgcattgga
    16261 acgtttgtat aatagccatt cccggcactc catcaataca ggaaaaccaa ttcctccgac
    16321 aaaaatcaaa aacatgatca ggatcaagaa aaaatagtca tgtgcaaagg gcttgatgga
    16381 atccccagtg acgtcaaacc cagagttcgt gacagccgat atcgcctgat aaaaaccgta
    16441 aaaaacggca tcacgccaac gatcaaaata gcctctgtaa taaaaataaa tagaaaagaa
    16501 tgtcccaaaa agtaattgaa accagattaa aatagcaaaa gtgatccgaa tcagtcggac
    16561 gataccagac aaacgcggtt gattcatatc cgtcatgatc agctgtcgct gtttcagtgt
    16621 gatccgtcgt ttagaaaaaa tgacaaaagc agtagaaatc atcatgatcc ccaaaccacc
    16681 aacttgaaat aatatttcca gcagtataat accgttatca ttgaacacag aattgatatc
    16741 aaaagtcgat agtccagtca cactcactgt actgattgcc atgaacagca tgtccacaaa
    16801 agaaacatgg ctatctggtt cgcgaaaaat cggcatataa aatagaacga gtgataagac
    16861 cgtcatcaat atataataaa aaacgatgat ttggatcgat gagaaatgat tggcagcaaa
    16921 cttctgtcct cgccgctgca gccttcgaaa gaataaatca acatgcacag tactatcctc
    16981 ctgacaatag tcgtttctac cagtatacaa ggggaattag gaatactcaa agaaattatc
    17041 aaacagataa caatacagct ttcgattaaa atagaaatgg cttataataa ggaaaatcct
    17101 agaaaaaaga aggaggagga ataacaaatt gattaatgct caacaaatcg tagaaaaaat
    17161 gaaaaatcaa aaaataaatt atgatacagt tctgaaaaaa atgatcatgc aatgggagaa
    17221 agaagaagta aagcctaaga tattaataca tacatgctgt gctccttgca gtacgcacac
    17281 attggaattc atgtctcaat atgcagagat cacgttattt ttttcaaatt cgaatattca
    17341 tcctaaaagt gagtatttgc gaagattaca tgaacaaaaa cgatttgtag accaatttaa
    17401 tgagaaaact gaccatcatg ttgcgctaat cgttgatgaa taccgtccaa atgaattcac
    17461 caaaatggtt ttaacaaacc atcttgaaaa cgaaaaagaa ggtggtgctc gctgcagtgc
    17521 atgcttcaat atgcgattag atcttgttgc gcaaaaagcg caagaactag gatacgatta
    17581 ctttggaagt gccctgacca tctcgccaaa gaaaaatgca gctttgatca atcagatcgg
    17641 aatggatatc caaaagattt acgggacaca atacttgcca agtgatttca agaaaaacaa
    17701 aggctatgaa cgctctttgc aaatgtgcaa agagtatgat atttatcgcc aatgttattg
    17761 tggttgcgtg ttcgctgcca agcagcaagg atgcgatctg aaagaaatca atcgggaagc
    17821 aaaagcttac ttaaaaacat caaccgtagt ttttgaggaa aattaaagaa atctttcgga
    17881 aaaacgtaag aaaaaaataa agaatttatg gtagaaataa ggtagactgt ataatttgtc
    17941 cgtagtaaag taaggaaaca agaataaatt tcaatgaagg aagagataag atgcgaaaag
    18001 tagcacctat tttgaagcca atcgctgtat taacaatctc gacgattgtc gcgactacta
    18061 caggtattgt gaatagttcc agctaccgtg caccaagttc aagttctagt actgtagaag
    18121 aaacaacacc aactacttca gtctcgacat ctgaaccaac aacaacggaa agtttgaatg
    18181 aaaccactac gacaagtaca tgggacgata caacaactag ttcgtcgtat gatgagacat
    18241 atacaactga cagctcaagc gaaacaagta atacggtacc agatggcact ccatcggatt
    18301 caaccaatcc aagtacaagt ccggatacaa gtgctagctc cgaaacgata ccaagcagta
    18361 cgactgtaga tgataacacg caaaatgatg atgcagcacg tacagcacag caaaaccaag
    18421 attcaggaaa tacccaaacg tataaccagc aagcaggtga ttctcaatga catttttact
    18481 agaccttatc aacggggtgc ataaattcct tggctattta gacatcagtc ctaaatatct
    18541 taaccgtgga tatacgattt taagtgtgat tcccacgctt tatttattga gaattgttta
    18601 tggactttgg caaaaccaaa actatcttca atttttctta tatggacttg catttctagt
    18661 gcttgtttat tttactgttt taaatgtatt ttattacttt ttagataaaa acacgaaagc
    18721 cgatgtcact cagctttttg tgaagtattt acctgatgaa gcgttcaaca tccagacaga
    18781 agaatcgata gataatggaa attcgatcga taaagtgaat acacaagagg tcatggtttc
    18841 ttatgacgaa gattatcaat tgaaattagc cgagaatatg cggtatttga ttggaaatgg
    18901 tgagatcaaa acaaatgaac tcggtcgcat ggatggattt ctggtcgata gaaatacatt
    18961 gtatccttat tactatgtca agaaaacagg agagaaagag tatgttctaa atattggacg
    19021 aagctactcg gacatggaaa aagtagggac cattcgactt gactcaacag aagacacatt
    19081 ggaacctgtc ggattgttca tcgtcggcgg tgattttgtc aaagacgggt tacgctatca
    19141 tgagccttat cgtctgaaat tgattgcaaa aaaggcacag tcgcaggaag aaccagtggt
    19201 ttatagtcgt agccaaagaa gaagacattc ataatgtgat gaaacgccgt gtatcagtgg
    19261 ttgcgataaa aacgtttatt cggaataagg gggatgtgac acgtcttttg tcacgttcct
    19321 tttttatata acgagaaaga taagacgaac acatcactta ttctttcctt tcatttataa
    19381 acattgtaga ataaagaaag tacaacgaag tattgtgaaa aaacgtaata aataacgagg
    19441 aaaatacaag taggaggtaa gctgtgaaag aatttttgaa aaattggggg attttgatcc
    19501 ttgtattagc ggctatttta ttagctagag tgtacgtgtt tacaccagtt actgtaaacg
    19561 ggcattcgat ggaccctaca ttaagtgatg gacaacgatt gatttcatct aagatctcaa
    19621 actatgaacg aatggatatt attactacaa aggaaccggg ggatgaagaa cggatgattg
    19681 ttaaacggat catcggtatg cctggagata cagtcaagat ggaaaatgac cagttgacca
    19741 ttaatggcaa aaagtatgat gaaccttatc tggatgagtt caaaaaagaa ttttcagaag
    19801 ataagttaca aggagaatat gcttatagct caggattcca agcacaagca gaaagttcat
    19861 ccacctttac gagtgacttt gaatatactg tgccaaaagg aaagtatctt gttctaggcg
    19921 ataaccgctt gatttcaaaa gacagccgaa tgtttgggtt agtcgacaaa gatatgatcc
    19981 aaggaaaagt tgttttccgt tattggcctc tttcagaaat taaaatcatg tgatgaaaaa
    20041 ataaaaagaa gctgtgacat gttttttgtc acagcttctt tttccgaaaa ttccttctta
    20101 tttcttggag ttgctcactt tttttgcaat ctcattttaa gctttttggc tgatggataa
    20161 gaaaagcgaa gcgtttttgt tcataaatag cttttcccca cgatggaagg tatgactagt
    20221 agcagagaca aagcccgttt tttccaatcg attattcaat tcttcttggc taaagccatt
    20281 atggactttc ggatgactaa tttgttcatt cttgtcaaag tctacgatga tcaattgacc
    20341 gccaggagct aaaatttcat aaagttttgt aagaatattc tccgtatcag gaatgtgtaa
    20401 aagtacaagt gatagaagga tgagattagc ttggacagca ggaaattcga cagaagcatc
    20461 agcgtggatc gttcggacat ttttcagatc tgctgcttgg atcttttctt ctgccatttt
    20521 cagcatagtt tctgaagcat cggcaatgat cagttctttg aatctttccg caagcggtaa
    20581 actaacaagc ccagttccgc ctccgtaatc cagcaggact ttttggtggc tgtcgcgcgg
    20641 tagataagta gttagttctt cgcgaataat cttggctaat gcttgacggt caggggaatc
    20701 ataatggtta gcgatggaat caaaaatatt ttttgtcatc aaatagctcc ttacgtaaaa
    20761 ctttgaaatt ttaacttatt atagcacgtg aaaagaaaga aagcatgcta tactcaattc
    20821 aaaaagggag aatctagaaa gaatatgccg atatcgtttg taaaagatag agaggaaaaa
    20881 ggaaagtgtg tccgagaaat attactagac ttgcctgagt ggtttgggct tcctgaatca
    20941 acggaaaaat atatcgagga atcaagtaaa ctacctttat ggtgtgaaaa aagaaaagaa
    21001 gaatatttag gctttatcac tctttcacag acaagtgaag atactgccga aatctattct
    21061 attgtatggg aataaaaaaa gactatcatc accaaggatt aggaacaaaa ctttttaaag
    21121 aagcagaagg ttacgcagca aaacattaca aatatttgca agtgaagact gtagacgagg
    21181 ggcactactc tatatatgat caaactattt gtttctacga atcattagga ttttcaaggt
    21241 tggaagtttt tccaaattta tgggatgaat ggaacccatg tttagttctt gtcaaaaaat
    21301 tggaacaaaa gtgagcaata gatttaatac taatctgtgc ttgatgaaaa ttctcactgt
    21361 aaaaaatgct gaaaatcaag tataatacca taggaacaaa ctggaaggtg aaaaagagca
    21421 tggcacaact attttttaag tatggcgcaa tgaatagcgg caagacgatc gagatcctaa
    21481 aagtcgcaca taattatgaa gaacaaaata agccagttgt cttaatgaca agcggtattg
    21541 atacaagaga tggtttaggc gttgtttcaa gccgaatcgg actgaaacgt aatgcgatgc
    21601 cgatatttga agaaacagac gtatttgacg taattcagaa aatggaaagc aaaccctatt
    21661 gtgtgctgat cgatgaagcg caattcttaa aaaagaaaca tgtcattgct tttacgagaa
    21721 ttgtggacga attggatatt ccggttatgg catttggttt gaaaaatgat tttcgaaacg
    21781 agttatttga aggatcaaaa tatttattgc tttatgcaga caaaatcgaa gaaatgaaaa
    21841 caatctgttg gttctgccac aaaaaagcaa tcatgaattt acattatatt gatggaaacc
    21901 ctgtttatga aggcgatcaa gtgcaaattg gcggaaatga agcttattac cccgtttgtc
    21961 gaaaacatta ctttcaccca tcaatgatta cagaaggaga ataagcatgt acgatcaatt
    22021 acaagcaatt gaagaccgtt atgaagagct tggagaactt ttaagtgacc cagaagtgat
    22081 ttctgataca aagcgtttta tgcagctttc aaaagaagaa gcaaacacgc gcgaaacagt
    22141 cgaagtttat cgtcgttata aagaagtagt acaaggaatc accgatacag aagaactgct
    22201 tggtgaaaaa ctggatgatg aaatggctga actagcaaaa gaagaattat ctgaattaaa
    22261 aaaagaaaaa gaagtcttag aagaaaaaat caagatcttg cttttaccaa aagatccaaa
    22321 tgacgataaa aatatcatca tggaaatccg tggagcagca ggcggagacg aagcagcatt
    22381 attcgctggc gatcttttca atatgtacca aaagtatgct gaagcacaag gctggaaaac
    22441 agaagtgatg gaagctaacg ttaccggaat cggtggatat aaagaagtga tcatgatgat
    22501 caccggagac aacgtatatt cgaaattaaa atatgaaagt ggtgcgcacc gcgtccagcg
    22561 tgttccttca actgaatcgc aagggcgtat ccatacttcc accgcaacag ttgtcgtgat
    22621 gccagaagct gaagaagtag agatcgatat tgaagacaaa gatatccgta cagatatcta
    22681 tcatgcttca ggtgctggcg gacagcacgt caataaaaca gcatctgctg tacgtttgac
    22741 tcacttacca acagggatcg ttgttgctat gcaagatgaa cgttctcaaa tcaagaaccg
    22801 tgaaaaagca atgaaaatct tgcgtgctcg tgtgtacgat aagatccagc aagaagcaca
    22861 aagcgagtat gatgccaatc gaaaatcagc agtcggaact ggggatcgtt cagaacggat
    22921 ccggacgtat aatttcccgc aaaatcgtgt gactgatcat cgtatcggat taactatcca
    22981 aaaattagat cagattttag cagggaaatt agatgagatc gttgatgcat tgatcatgta
    23041 tgatcaaaca tcaaaactgg aagctatgca aaatgggtaa aatgacctat cgagaagtcc
    23101 tgtcacgggc ttcttctttt ttagaggatc atgggaaaga agcatatagt atccaatttt
    23161 tatttttgga acgaaaacaa tggaaaaaac ttgattggct ccttcatatg aacgaagaga
    23221 tttcagaaga agaacagcgg ttgatcgaaa ccgatctgcg acttctgctt gcagatcatc
    23281 cacctcagta tttgctagga tatgcagatt tttatgatca tcggttaaag gtaactgaag
    23341 caacactgat ccctcgacca gaaacagagg agttagtcga atggtgttta gacgagacgc
    23401 ctggtgtacc gttagaagtg atagatattg ggacagggac tggagcaatt gctattagtt
    23461 tgaaagcagc aagaaaaaat tggcatatat ctgctgtgga tctttcagaa gaagcattag
    23521 aagtagcgaa agaaaatgct cagaaagaag gaacgaaaat cagtttttat catggagata
    23581 cattaggacc agtaatggat cagcaatttg atgtgattat ttctaatccg ccgtacatca
    23641 gtcggaatga atgggaactg atggatgaaa gtgtccggtc attcgaacct aaaatggccc
    23701 tttttgctga gaacgatgga ttagctattt atgaaaaaat agcagttgaa gcaccaagcg
    23761 tactaaaatc aaatggaaag atttttttag aaatcggatt tcgccaaggt gaagcagtaa
    23821 aaaaaatatt tcagcaagca tttccagata aaaaagtggc gataaaaaag gatctttttg
    23881 gcaatgaacg aatgatccaa gtttatggtt gaagttgtgg atgaaaaatt ttttatttgt
    23941 ttttttatta taaaaaaaca cttatttgac aaggatgtaa acgacttttt ggtttatcaa
    24001 caagtttatc cactattgac aaatagatta tccacaaaca gtggaaaact tgaggataaa
    24061 ctcagacaaa ataagaatgt atgaaaaagg ggttgtggat aaactgtgga aacgaaacga
    24121 tatgatgaaa cagaattgca agaggcagcc gaagcactga aagaaggaga acttgttgct
    24181 tttcctaccg aaacagtata cggattgggc gccaacgctc ttttaccaaa cgctgtcaag
    24241 aaagtgtttt ctgtaaaagg gagaccgcaa gataatccat taatcgtcca tgtcgcctct
    24301 tttgaacaag taaaagagta tgtggacaac tttcatccgg cagcagagaa gattgtgaag
    24361 aacttctggc ctggaccatt aactttgata tttaagatca aaaaagacac tttgccctca
    24421 gttgtgacgg gaggattatc cacagctgca tttcgtatac cggataacaa aaaaacacta
    24481 gaagtaatcg aactttccgg tgttccgctt gtcggtccaa gtgcaaatac gtcgggaaaa
    24541 ccaagtccaa cgactgcaga tcatgtatat catgatctac aaggaaaaat taccgggatt
    24601 attgatgatg gagctacgcg aattggtgta gaatccactg ttcttgattt aagtgatcca
    24661 acagccatgc ctatgatttt acgaccaggt gcagtaacga aagaacaaat cgaagcagtc
    24721 atcgaatctc ctgttgctat tgatcagcat cttgtaaaag aaaatgaaac accaaaagcc
    24781 cctgggatga aatacaaaca ctactcaccc gatacacgtg ttctgatggt cagagatggc
    24841 gattggtcaa ctgcagttca atgggcaaaa aataagaaga tccgtgcagg tgtcatagca
    24901 agccccgaaa ttgcggatca agtgcggaca gatactgcag ctgtttatat gtataacgac
    24961 aactcagtag aagcggcggc taaaggattg tttgcaggtc taagaggatt agatgaacca
    25021 acacttggat tagatttgat ttttgtacaa gtgtatccag aaactggatt ggggaatgct
    25081 tacatgaatc gcttgaaaaa agcagcagga caaaattatt ttgaaaaatg atcaattggt
    25141 tattgtcgca ttcttttagt atgatacaat gctatcaagt aaatcttcta tcagtgaacg
    25201 aaaaactaag cgtaactaat cgtaatagaa gagatacgaa tatttggcaa tttgtttttg
    25261 atgggaaaaa taaaattgta ggaaaataaa aaggagctgt tgtttgtggt agattacaaa
    25321 acgtttgacc ctgatctttg ggctgcgatc gccaaagaag aagaaagaca agaacataat
    25381 ttggagctga ttgcctctga aaactttgtc tccgaagcag tgatggctgc acaaggaagt
    25441 atcttgacaa ataaatacgc agaaggttat ccagggcatc gctattatgg tgggtgtgaa
    25501 tttgtcgata tcgttgaaaa tttagcgatt gaccgtgcaa aagaattatt cggagcgaaa
    25561 tttgcgaacg tccagcctca ctccggctct caagccaaca cggctgctta tctcgctttg
    25621 gtagaaccag gggacacgat cttaggaatg gatctttccg caggcggaca tctaacacat
    25681 ggatcaccag tcaacttcag tggcaaaacc tatcactttg ttgcttacgg tgttgatccg
    25741 acaacagaag ttattgatta taatgtggta cgtattttag ctagaaaaca tcaaccaaaa
    25801 ttgatcgtag caggtgccag tgcttacgga cgaacgatcg actttgcgaa attcagagaa
    25861 atcgcagatg aagtaggcgc aaaactgatg gtagatatgg cacatatcgc aggattagta
    25921 gcagcaggac ttcatcctaa tccagttcct tatgcagata tcacaacgac taccactcat
    25981 aaaacattgc gtggccctcg aggcggcatg attttgacaa acgatgaagc attagcaaaa
    26041 aaaataaata gtgcggtatt tcctggtatt caaggcggcc ctctagagca tgtgattgcg
    26101 ggtaaagcag tagcattcaa agaagcatta gatcctgcat ttaaagaata tagtgaacaa
    26161 atcatcgcca atgcaaaagc aatggtcaaa gttttcaatc aagcaattgg aaccagagtc
    26221 atttcagggg cgacagataa tcatctgatg ttgatcgatg ttcgcgagtt aggtataaat
    26281 ggaaaagaag cagagagcat tttggacagt gtgaatatca ctgtaaataa aaactcgatt
    26341 ccttttgaaa cactaagtcc attcaaaaca agcggtattc gaatcggcac gccagcaatc
    26401 actactcgcg ggttcaaaga agaagatgct gtaaaagtgg ctgaactagt tgtcaaagca
    26461 ttacaagcaa aagatgataa cgctcaacta gacgaagtca aaacaggtgt acgcgaatta
    26521 actgaaaaat ttccgttgca taaaaaataa aaatttaaat tccattagta aacagaaaag
    26581 ttcaatgttg ggcttttctg tttatttttt gggaaaacgg attctttgac aatatcggtg
    26641 atgctctcta gtatatgagg tgtaatttta gtcttcacac taaatctcag ggaggaaaag
    26701 tcatgactga tcgtatctcg ttagaaaagg aagcagtagc attcagtgat gcaaatgcgc
    26761 ctcatccgcg tatctatgaa ctagcaccgg aagaaggaag agttttacta gaagaagtac
    26821 aagaatcacc tgtgaacaaa tattccgtcg atattgaaga cttgatggtg gatactggcg
    26881 aatggggaaa actcaatatt cgtctcgtac gaccagaagg gaatatggat aaattaccag
    26941 ttatttttta tattcatgga gcaggctggg tttttggcag tgctcaaacc catgataaat
    27001 tgatcagaga acttgcggtt cgtacgaatt ccgtcgttgt ctttccagaa tatagtcgat
    27061 caccagaagc aaaatatcca acagcaattg aacaaaatta tgctgtgttg caacagctga
    27121 ataaattagc caagtcaaaa aatctggatc tcgagaatct aatcgttgca ggagattcag
    27181 taggtggtaa catggctaca gtcatgacga tcttaacaaa agaacgaaag ggcctcccaa
    27241 tatccaagca attgctgtat tatccagtca caaacgctga ttttgataca gattcctatc
    27301 atgaattcgg agaaaactat tttttgacaa gagaaggaat gatgtggttt tgggaccaat
    27361 atacgacgaa tgaagaagag cgtgctgaaa tcacagcttc gcctttgaga gcaaatataa
    27421 atgaactaaa tgatcttcct tcagcaatga ttttaacggg agaagcggat gtattaagag
    27481 atgaaggtga ggcttatgcg cgcaaacttc gtgatgcagg ggttcctgtt acacaagtac
    27541 gcttccaagg aatcatccat gattttgtca tggtaaatgc tatggatcag acaaaggcaa
    27601 cgcgtgcagc aatggcatta tctacttctt ggatcaagaa aaaagaataa gctttgcttg
    27661 tccgcaggaa aacttctttt caaaaacgga ttttcttagg aaaaatggcg agtttgacta
    27721 gtcaatcact tgattttgtt ttacaattaa aaagaaacga cagaacaaag gagagcttaa
    27781 aaaatgggca aatttcaagt gatcgatcat ccattgattc aacacaaact aactattatt
    27841 cgggacaaaa actgcggaac aaaggttttt cgtgaagtag tcgatgaaat cgctatgttg
    27901 atggcatatg aagtatcacg ggatatgcca ttggaagaca tcgtgatcga aacaccgatc
    27961 actgaaacaa cacaaaaaac gttgtctggt aaaaaagtag cgattattcc aatcttgcgc
    28021 gcaggtattg gaatggttga cggtatcttg gaattgattc cagctgctaa agtgggacat
    28081 atcggtcttt atcgagatga agaaacattg gaaccacatg aatatttcgt caaacttcca
    28141 gaagacattg atgcacgtca gttgtttgta gtagatccaa tgttggcaac aggcggatca
    28201 gcaatcatgg caatcgatgc attgaaagaa cgtggcgcgt cgaacattaa atttgtttgt
    28261 ttggtagcag cacctgaggg agtaaaagct ttgcaagaag cgcatcctga tattgacatc
    28321 tatacagctt ctctagatga aaaattgaat gaacatggtt atatcgttcc tggactaggg
    28381 gatgcgggtg accgtttgtt tggtacaaaa taagtaaata cgaaaaaggg tgtgacacaa
    28441 cttttgtcac atcctttttt ggtcattttt aacgtttgat gcagaacagc tttttcacag
    28501 ctttcgtttg catataaaaa aaccatccca aaaatgcaga gatggtcaaa ttcaaatctt
    28561 ttttagttct ttgtagttaa cgattactac acccttactt tgcgtcatca agttcgcatt
    28621 ttctgcagtt tcatcaatct caacaacagc ggaatttttc aattgctttg aaatcgttcc
    28681 agtttgcgac ttgccgtgaa ttgtgaacat tacgtgagta ttgatcttga atttttcacc
    28741 aatttccgat ggtacgatag ttgtatcaaa ttttccaaaa gttgccattt tcataaaacc
    28801 tgcctttcac atcaattata ccacgtttta tgaaaaatat catgaaatta aactaatttt
    28861 ccatcggact ctttacaatc agccaaatag agtatataat ttcttttgga taaggccagc
    28921 ttagctcagt tggtagagca acgcactcgt aacgcgtagg tcgtaggttc gatccctata
    28981 gctggcatta aaagagaaga cagatatatc aaggattttt aatccattga tacatctgtc
    29041 tttttgttta aaggaaataa agtatgcttt cttagcaata gagcatgtat tatttctata
    29101 aaaatttagg agaataaaac gactagcaat atagctgtga aatcaaatat cacatgaact
    29161 gcccaacttg taatgatcga gttgctttta atggcagctt ggttgaataa caatcttgcc
    29221 accccttgta tcaaaaggat gtgtgctaca gtgtgtaaaa tatttccatt atcataagta
    29281 ctgtaatgga taaatccaaa gatgattgct gacaagaaac tagcgacagg aatgctgaac
    29341 ttactagaaa aaatagcgag aaaatacact gagaacaatt cttctccaag tatcatgata
    29401 ggcaaaataa taaataataa cgggctgaaa gcctcattta ttggattgcc tgttaaaccc
    29461 catttcaaaa tttgttgcaa aaataccgag actgcggcac tgattactac attgataaaa
    29521 aaatatttgg ctatattttt gacaggagcg acaggtttcg agaacatttt tttcgtatct
    29581 tttttaccaa aaacaagtgt aaatgctata aatccagtga tagttaaaga aaaaatgcta
    29641 ataatggatg gcgcaaatag caaccctaac aaaccgagta caacgacaat agtgcccaat
    29701 atcccttttt taagttctat tttttttcct aaaatgttat tcatgataat aactcctact
    29761 attcagtttt tgaagataga gtcagtatac atgaagagtt aaaataggca atgttttttt
    29821 atggatattt gtattgctaa aaatttatct tatatattca ttttgattat actactttta
    29881 tttgtaaaaa ttttctagca aaaccaagtg cttgcaactg attaagcgta caatttttta
    29941 gatgttccga gcgtacttca atcatttcaa aggagttgct acttaaatcg atatttttta
    30001 aatcagtatc aaaaaagcta gcattttcta aatcattagt agaaaacatt attttagtga
    30061 gggaacagtc tacaaaatct gcgcttgata agacagagtg ctcaaaggta ctttctttta
    30121 tgtttgaacc agataaattt acatagtcca ttttacaatt ctcgaataac gtgttcccaa
    30181 tataactttc catcaattta gtacctgtta atttacaatt gacaaatttc acgcgatgca
    30241 acatgctttt tgtcaatata cagcctgaaa aatcacagtt ttcgaatata cagtcggtaa
    30301 tctccattct ttgaaaatca ttggaagcaa aaaatgcata cttgaaaata gatttgtaaa
    30361 taattggatg gcagattatt tggttttcga tgacctcttt ttgtgaaagg atattataca
    30421 gttcaaaatc ttcgttttca tagatatgaa aaaaggatcc tttattcaag cttgcttcat
    30481 taactttagg aaaaatcata tttaattacc tccattttca taaagtcata ttaacaaaag
    30541 ctaaaccaac ttacattagc agaaaatgag ataggttttc gcctttttcg ataacgatag
    30601 tgtcgctatc tcttgaaacg ttcttagaat tgccctgaga gacacaaatt aggcgtaatt
    30661 ctaataaaaa tcttagaata aaaattttta ctgatttaat ttagaatgtt tttaaaataa
    30721 aaatcgttgc cataacagtg tattgaaagg ttttaacaat ccagtaatga tttaaaaaaa
    30781 tctagatgta gcgtatactt tgtttataga atgtagtggg aagaaatata tttttgggag
    30841 gaaatcgtga tgtctaaaag tgaagaaata gaaaacatgg tactaaaatt atatgatctc
    30901 gttttaaatc cagagatcaa agaaaaggaa cgtatacttt tgatagacgc aaagacaggc
    30961 ttagaaaaag gtcagtatta tcctaaagtc ataaataatt tggaacgtag tttacgtccg
    31021 ttagcaatca ggggagaatt gtctaaacca gtatcaccgt tttatatgga gatatcaaca
    31081 ataggaaaat ttgaaaaaga attaggtcga ggaatggctt cggcaccgat tacttttggg
    31141 catttataaa aacgagttat tcatctcaat gaattaaaaa gtatagtcaa tctcataatg
    31201 caggctaggt tcatttattt gaacagagtc tgtatttttt tattctaaat aaagaaacag
    31261 atgtttcttt atattgtatt ttatccttta attaacacaa aaacagctag tataaactaa
    31321 taatgttaat taaaagtgta ttttatgaaa ctatcgttat ttattaatgt tgcatttttg
    31381 taagttaaaa attggaaata tagattgaaa aaatgaaagc aataagtaaa ataaaatgaa
    31441 tatatgcgaa aaaataattt acattaggaa aaaccttgta aaaacagttt tttggcatat
    31501 ttatttttta taaatatttg taaaataact atcaattttt tagttaaaag attggaaatg
    31561 agtgggggag gaattgaaga aaggattttt gagagatgat atagtaggtt ttatttttag
    31621 ttaaaatagg ggagatagaa aaaatgaaaa agtgtagaaa tattatttta tcaatgttat
    31681 ttattattac taatataaca tcactgattc ctgttcatgt ctatgcggat gcaggcagag
    31741 atatcagcag taatgtcact tcgttgaccg tggacccaac gaatattaca gatggaggaa
    31801 acataaaagt aaaattctcg tttgacgaaa aaaaacaaaa tatccaacca ggtgattatc
    31861 tttggatcaa ttggccaagt gaaggaaata ttcgcggtga aggattccaa aaagaaatcc
    31921 cattaatgat tgaaaataaa aatgttggaa ctttaacggt cagaaaagat tctgcgcagg
    31981 ttgtgtttaa tgaaaatatt aaaaacctag actcagtaga aggttggggg caatttgaga
    32041 tacaggccag aaacgtaacc gataccggtg aagaaaatac gggaccattc accgtgacga
    32101 gcggtgataa aacagctact gtaaatgtga caaagccagc ttctggttct tcttctagcg
    32161 ttttttatta taaaacagga gacatgctac ctgaagatac taagcacatt cgatggtttt
    32221 tgaatattaa caataacggc acttatgttg aacaaccagt taaaatcagt gatgaaatcc
    32281 aaagtggaca aagattagat ccaagtacgt ttgaaattaa tcaaatacac ttaggtgaac
    32341 agaaggttta tagaggagaa gagggaatcc aacaattttt acaagatttc cccagcgcta
    32401 catttaattt ttcagtaact gataattaca ttgaaataac tataccaaaa aactttgtca
    32461 atcttaggaa aataatggtg agttataaga cgatcataga aaacccagaa caaataaatt
    32521 ttgagaatca ttcggaagct tggtttaaag aatttaataa acctgctgtt gatggagaaa
    32581 gtttcaatca tacagttaaa aatatttcag cttctggtgg agtcaacggt acagttagag
    32641 gcgaattgaa gattttcaaa tatatcaatg acacagagat tggtatccca aatgtgactt
    32701 tcgaattaag acgagcagat gaacagccta ttcaaggaca gtctagtatc ctgttgactt
    32761 cgaatgaaca gggagaagct agtatcaaag gcctgcaagt aggagactat gttgtaaaag
    32821 aaaaagaagc acccaattgg attgattttg atccactaag ttcaaatgaa ctcaaattct
    32881 ccataaacga aaatgataca gagggagtca gtttacctat ttataacaag aaaaaggtta
    32941 cgaacatcac agcaactaaa atttggaacg gtggaaccac tccaagaccg agtatttatt
    33001 ttaaattatt cagagcaagt acaaacaatt gggaaccagt tcctgatgca gaaacaaaac
    33061 gattagacaa tggaataact tcagtgactt gggaagatat ccaacagtat gatgatagtg
    33121 gaaacgaata tacgtttaaa gtgcaagaag tagatcaaaa cggaaatgat tatgtccctt
    33181 caggttatca aaaaattgaa aacgggctag tcgttacaaa tgagaataaa gaagtcattt
    33241 cagtctcagg acaaaaaaca tgggaagaca atgagaacca agacggcaaa cgaccgacga
    33301 cgatcacagt aaatctatta gccgacggtc aaccgatcca acacaaagaa gtaagtgaaa
    33361 aagatgattg gcgctatcgt ttcacaaatc tgccaaaata cagagacggg caagaaatca
    33421 tctatactgt aacggaagac aacgtgcccg aatacagtac gacgattgac gggtataaca
    33481 tcaaaaacag ttatacaccg gaaaaaacaa atatatctat accaggtagc catataacac
    33541 cgtggaaaaa ttttccagag aagacagaga gagagaaaat tagaaatcta ttctctcaaa
    33601 aattgcaaat atttgctcat acaggagaat ccagcgaaca aagaagcaat tatattagta
    33661 agagagctgt acctaaacag ataacaaata aatcaaaaag cattcttcct aaaacaagta
    33721 gtaaaaaatc tagttttgct gtaatcattg gtttattgat agtaactatc tgtgctggga
    33781 tattcttaca aaaatataaa aaagtttagt tagaatataa tattttttgt taaatatgac
    33841 tgaaatcaaa agaataaaat tccgatttat aatcgaaaaa agaagcattt ttatatatgc
    33901 ttcttttttc gtagaatcaa taaatatgga tttggctaac tatggaagaa tactcgtatt
    33961 agttttcagc gttcatacta gttgttacat tgtttgattg gccaaaagaa tttctgatat
    34021 attttgtgct aaataaagaa aaagacgctt gaattgggaa ataagaaaac tactctatca
    34081 ttacaaattt tcataaactg gcagatgttc taagtaaaca agtatctttt caaagtcgga
    34141 aaaatccata atcaacgtag tggtattgat atttggatga aagcctacgg tatcttgacg
    34201 atcaatagca gcatcgatca cgacttggat ttttttctct ttgtcgtgca tcaatgctaa
    34261 tggagtcact gtacctgctg gaacgcctag gaggtcagga agctgttctt cagaaacaaa
    34321 agataagcgc ttttctttta attgttcagc tagttgtttt aaatcggcgt gcttttcatc
    34381 aggcaagatg acaagataga tttgttttcc ttttcttgat ttcaataata gattttttac
    34441 ttgcggacca ggtagggaaa aagggacatt tttgactgac gtaatcgcag gatgatcgac
    34501 tttttgaaaa gagataccca gtttttttag tagttcataa gcttcttgtt cggttgcacg
    34561 actcatcgcg catcaccctc ctttttttat tagtgtatca taaaaaagag ttgacaaatg
    34621 aaaaataatc atgtaaatta cacgtaaagt taaaacaaat gacatcgaaa agaagagtag
    34681 ccgttaggtg tttttagaga gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa
    34741 cacatcttgg agtcgatttt ctgaagcgta tagtaggaaa atccggggag cccgttacag
    34801 ctgcggtcgt tgctagatga ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa
    34861 aggtggaacc acgtgattaa cgtcctttga tcttcggatc aaaggacttt ttgttttcat
    34921 ctgaacggca acgactgtgt gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac
    34981 cacgtataat acgtcctctt ggcatttgcc aagaggattt ttttatttta aaggaggaaa
    35041 agaagatgga gtatttgatt gaagttatgc cgcaattatt agtaggagca ctcacaacat
    35101 tgaaattatt tttatttacc ttgattggct cattaccatt agggattgtt ttagcattag
    35161 gattgtcttt gcattttaag ccgctacgct acttgcttca aacatatgta tgggttatgc
    35221 ggggaacacc gctgttgttg caactgattt ttatttttta tgggttgccg acgattggga
    35281 tcgtttttga tcgtttcgaa gcagcactca ttgcctttat cttaaactat gcagcttatt
    35341 ttgcagagat ctttcgtgga gggatacaat cgattcctca aggtcaatat gaagcagcgc
    35401 aagttttgca attaagtccc tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa
    35461 agattgtttt accttctgta gggaatgaag ttatcaactt ggtcaaagat tcttccttga
    35521 tctatatatt aggtttaggt gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg
    35581 cgactttact gcctttgcta ggcgtggcaa tcatctatct tacattgact ggtattctaa
    35641 caatcgtaat gaaacaagtt gaaggaaaat taaattatta ccgctaactg ataggaggaa
    35701 gaaatatgat tgaattgaag aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag
    35761 atttgaatct aatcattcca gatgggaaaa cattagctat cgtaggtcct tccggtggcg
    35821 gtaaaaccac gcttttacgt atcttggctg gcttagaaag ttctgacagc ggagaatttt
    35881 atttagacgg tgtaccgttc aacccattcg agcttaaaat gaaagaacaa gttgtaggag
    35941 tcgtattcca agacttccag ctatttccac atctgaatat ttttgaaaac attaccttag
    36001 caccaaaaat ggtattgaaa cagacaaaag aagaatacac caaaaaagca gaagaattag
    36061 cggcttcatt agggattagt gatttattga atatctatcc ttatcagctt tctggcggac
    36121 aaaaacagcg ggtagcgatt gccagagcat tggctatgaa tccacgggta ttggcttatg
    36181 atgaaccaac aagtgcttta gatcctgaat tacgccagca agtcgagcgt ttgatcctat
    36241 cattgaaaga agatggggta acccaaatta ttgtcaccca tgatttagta tttgcagaaa
    36301 atgtcggtga tcaattacta aaagtttccc caattcatta agaaaggaga agatagagat
    36361 gaaaaaaacc agttttctat tcgctttgat cgcaggtctt cttctttttg caggctgttc
    36421 gaacaaaaaa acttcagcag ataattgggc gactatcaac gaagagaaaa aaatcgttat
    36481 aggtttagac gatacatttg ttcctatggg atttagagat aaagacggga agctcacagg
    36541 atttgatatt gatttagcca aagcagtctt tgaacaatat gggattacgg tagactttca
    36601 gcccattgac tggtcaatga aggaatttga actgaacaat ggaacgatcg atttgatatg
    36661 gaacggctat tccaaaacgc cggcaagaga aaagaaagtg caatttacaa aaccttatat
    36721 ggaaaacgat caagtgctga tcacacctaa agaatcagga atcacaaact ttggacaaat
    36781 gaataataag agattaggtg cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca
    36841 accagaagta ttaaaagaga tagtcaaaaa ccaagatgct gttttgtatg atagttttaa
    36901 tgaagcattg atcgatctga aaagcagacg ggtcgatggt ctgctgatgg ataaagtata
    36961 cgcagactat tatttgaaac aaagaaacga actagaagac ttcaacataa caaaaggttc
    37021 ttatgagagt gaggattttg cagttggtgc acgcaagtca gatagtacat tagtgaaaaa
    37081 aatcaatgaa gccctagatc aactagagaa aacaggtgaa ttccaaaaaa tatccgataa
    37141 atggtttggc gaagatgtta cgccgagaaa ttaacaaata ggggactgtg acaaaagacg
    37201 tgtcacagtc cctttttccg aataaacagt gttctcctgc caactccttg tgactcggag
    37261 ctgaatggca ggctcacaac ctcttggtaa acggtgttca aaagccagtt tttcggtatt
    37321 aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct tactactcga
    37381 aactaagcga ctttttcaca acctccttta cataatttta ttttgttgtg gaaggggttt
    37441 cgtgttattc taaaagagga acgattgaag gttataggaa aggaagagat gaatggaaca
    37501 caaagaactt acctcatttc caaaagattt tctgtggggc tctgcttctg cagcgtatca
    37561 agtagaagga gcttggcaag aagatgggaa aggagaatca gtctgggatc gatttgtacg
    37621 gatacctgga aagacattta aaggaacgaa cggtgatttg gcagtagacc actatcatcg
    37681 ttataaagaa gatattgctt taatgaaaga acaaggtctg aaagcttacc gcttctctgt
    37741 agcttggaca cggatttttc ctaatggacg aggagaagtc aatcaagctg ggctcgcttt
    37801 ttatgagaga ttgatcgacg aattgattga aaacgatatt gaacctatgt tgactttata
    37861 tcattgggat cttccacaag cattgcaaga tgaatataat ggttgggaat cacgtcagat
    37921 cattgaagat ttcactcatt atgcagaaac attatttgag gcattccgcg ggaaagtcca
    37981 ttactggatt agtttgaatg aacaaaatat cttcacttca ttaggttatt tgttagcagc
    38041 acatccgcca ggtgtgacag acccgaaacg aatgtatgaa gttaaccata ttgctaattt
    38101 agcaaatgca tctgtcatca ataaatttca cgagttagaa atgcctggaa aaatcggacc
    38161 aagctttgcg tatacgccaa attatccaat tgacagtaat cctgaaaatg ttctggctgc
    38221 tgaaaatgca gaagacctga tggccaatta ttggatggat gtctatatgt ggggaaaata
    38281 tccgattgct gccatgaggt tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg
    38341 cgatgcagaa ttgctagaat ctgctaaacc agattttctt gggatcaatt actatcaaac
    38401 ggcgacgaat gcattcaatc cattaaacgg agttggtgct ggaaaaatga atacgacagg
    38461 aaaaaaaggt tcatcagaag aaactggagt tcctggaatg tataaaaaag ttgagaatcc
    38521 atttgtcgaa cgaacaaatt gggactggga gattgaccca gaaggattaa ggatcggact
    38581 gcgtcgtatc acaagccgtt accgcatgcc ggtactgatt acggaaaatg gcttaggaga
    38641 gtatgacaag ttgacagaag ataaacaaat ccatgacgac tatcgaatca attacttaca
    38701 aagccacgtc aaagccatca aagaagcaat ttcggatgga gcagaagtcc taggctattg
    38761 cacctggtct tatacagatt tgcttagctg gctgaacggt tatcaaaagc gatatggatt
    38821 tgtatatgtt gatcaagacg aaacacaaga aggatctttg gaacgatata agaaagacag
    38881 ttattactgg taccaaaaac tgattgaaga aaatggagag aacctttagt tcacatactt
    38941 aaaacaagaa ggtacggctg gatatggccg tgccttttct tgataaaatc atttagaaat
    39001 taagaaaaac tgtaaaataa gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa
    39061 ctttagttcc cttacttttt tttgtataat gaaacttgat gaaatgaggg ataataaatg
    39121 gcacaaaaga aacggcctac taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa
    39181 cagcagcagg aacaaatgct gacgattggt gttgggtttg ccttgatttt atttgctgtc
    39241 ttcggctttt taaaattagg ttttttagga atattgattg caaacggctt tcggatcatt
    39301 gcaggaaata cttaccaaat cctctgtctt ttattagccg tattaggatt ttggattgtc
    39361 attaagaata cagagttttc aattggtaaa aaccgacgct ggtttggcgg catactcttt
    39421 tatttcggta tcttattatt gttacatgcc catttatttg gcaaactcca tactggggaa
    39481 ccaaatatca tggggacgac atgggatttg ttagccagtg atataaaaca aagtcaagta
    39541 gacaataacg ttggtggtgg gatgattgga gcaatccttt atcattttac ttacttcttg
    39601 attgcacagc ctggaagtta tttagcagca gtgctattac tggcaggagg agcattcctg
    39661 ttcagtaatc tggaaggcta tcagctgtta aatggtatcc aatcaattgg agaacgagta
    39721 caggaattat tggaaggtga tcctgccaag caagctcgaa agcaagcggc aaaggaagag
    39781 agaatgaaac agcgcgcaga agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca
    39841 gaaaaagaag ctgcagaata tgagaagaaa aaagcggtga gccagcaaag ggaaaatact
    39901 gctgatgaat ggcaggaacc agaaaataca gagccggagc agctaagttt tgttccaatc
    39961 gacagttttc aagaaaatat ccatcctgcg aatctagaga aaccagtacc agatacacca
    40021 aaacagacca atacagcaga aggtttttca gatgagttgc cagaggatga cggtacctca
    40081 ttagaatttg agattgaagc agaacaggaa aatcaagact atgaattacc gacagttgat
    40141 ttgcttgatt ctataccaac tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat
    40201 atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc
    40261 agcttaggac catccgtcac aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt
    40321 aaaattgtga acttaacaga tgatatcgcg cttgctttag cggcgaaaga tgtccggatg
    40381 gaagcaccga ttccaggtaa atcactgatt ggtattgaag taccaaatgg aaagatcagt
    40441 atggtttcct ttagagaaat catcgaagca caaccaaacc acccagataa attactagaa
    40501 gtacctttag gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc gaagatgcct
    40561 cacttactcg tggctggttc gactggtagt gggaagtcag tagcaattaa tgggattatc
    40621 acaagtatat taatgcgtgc taagccccat gaagtaaaac tgatgatgat cgatccgaaa
    40681 atggttgaat tgaatatgta caacggcata cctcatctgt tgacgccggt cgtgacgaac
    40741 cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga acgatatgaa
    40801 aaattcgcgg caaccggagt aagaaatatt tctggttaca atgaatttgt tcaacagaaa
    40861 aacttggaaa acggaacgaa acatccaacc ttaccattca tcgtagtgat cgtagatgaa
    40921 ttagctgatt tgatgatggt agccagcaat gaagttgaag atgcgattat tcgtttagcg
    40981 caaatggctc gggcagcggg gattcatatg atcctagcta ctcaacgtcc aagtgtggat
    41041 gttatcacag gaatcatcaa agcaaatgta ccttccagaa tggcatttgc ggtatcaagc
    41101 ggtacagatt ctcggacgat cattgattct aatggggcag aaaagctctt agggcgagga
    41161 gatatgctat tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt
    41221 tctgaccacg aagtagaacg ggtcgttcaa tttgttacag atcagcaaga agctcattat
    41281 gaagagaaaa tgatgccgac agatgaagtt gaaacgacag gtgctccaga acagccacaa
    41341 gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc
    41401 cttctccaaa gaagatttcg gatcggttac aaccgagcag cacgtttagt agatgaactg
    41461 gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt attcattgag
    41521 caggaagaaa catcagaaac gttgccggac gataatgaca tgtaaaactt gtgtgatgaa
    41581 gggttgaaaa acgaataacc aataaaaaga caaggcatga tgataccaaa agaaaaaatg
    41641 gtttttttcc atcttttcta atacggtatg atgtcttgtc tttttagttt ttgtgagatt
    41701 aaagttctat tcgatttatt tgtgaagtat tgcattaagg aaatgaatca tgtatagtat
    41761 gtagcgaaac tttgagaaaa agagggaaaa tactttggaa aaacagtcca tttatggctt
    41821 gacgaatgaa gaattaatca attggtttat agaaaacggc gagaaaaaat tccgagctgc
    41881 ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct
    41941 atcgaaacaa ttgatcgaaa aattatcgga gcattttatc atcaatcctc taaaacagat
    42001 ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg tttgaactcc ctgataaaaa
    42061 catgattgag actgtactga tgagacaaga atatggcttg tcagtttgtg tgacgacaca
    42121 agtaggatgt aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga
    42181 tctgactgct ggagaaattg ttgcacagat catgatggtc caacattatt ttgatgagcg
    42241 caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc catttgataa
    42301 ttacgacaat gtaatggact ttttgcatat catcaatgat gccaaaggat tggcaattgg
    42361 tgctcggcac attacagttt ccactagtgg attagcacac aaaatcaagg aattcgctaa
    42421 taacggcttg caagtcaatc tggctatttc cttgcacgcg ccgaataatg aggtgcgaac
    42481 atcgatcatg cggatcaacc gaagtttccc aattgagaaa ttgatggaag ctgtggatga
    42541 gtatttagag aaaacaaacc gtcgaatcac gtttgaatat atcatgttga atcaagtaaa
    42601 tgatagacca gagcatgcgc aacaattagc agatctatta aaagataaga aaaaattagc
    42661 atatgtcaac ttgattccat ataatccagt cagtgaacat gatcaatatt ctcgcagtcc
    42721 aaaagctgat gtattgagat tttatgatgt attgaagaaa aacggcgtaa actgtgtgat
    42781 acgtaaagaa catggtacag atattgatgc agcttgcgga caattgcgaa gcaagcaaat
    42841 gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt ttcaattacc ataaaaggag
    42901 cagttttctt aattaaggaa ctgttcaact taaataagag aaaagattct atagcgaaag
    42961 ctataaagct ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga
    43021 tgacagcctg cttgatgggg gttttagcaa tt
//
LOCUS       DACEUF010000014        42486 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.015, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000014 DACEUF010000000
VERSION     DACEUF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.015"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            75..521
                     /locus_tag="IXG20_000746"
     CDS             75..521
                     /locus_tag="IXG20_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1608960.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(580..1041)
                     /gene="mscL"
                     /locus_tag="IXG20_000747"
     CDS             complement(580..1041)
                     /gene="mscL"
                     /locus_tag="IXG20_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ1608961.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            1293..2561
                     /locus_tag="IXG20_000748"
     CDS             1293..2561
                     /locus_tag="IXG20_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1608962.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2554..3840
                     /locus_tag="IXG20_000749"
     CDS             2554..3840
                     /locus_tag="IXG20_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1608963.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            4618..5502
                     /locus_tag="IXG20_000750"
     CDS             4618..5502
                     /locus_tag="IXG20_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1608964.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            5711..6289
                     /gene="pgsA"
                     /locus_tag="IXG20_000751"
     CDS             5711..6289
                     /gene="pgsA"
                     /locus_tag="IXG20_000751"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ1608965.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            6529..7770
                     /locus_tag="IXG20_000752"
     CDS             6529..7770
                     /locus_tag="IXG20_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ1608966.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            7879..8928
                     /gene="recA"
                     /locus_tag="IXG20_000753"
     CDS             7879..8928
                     /gene="recA"
                     /locus_tag="IXG20_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ1608967.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            9339..10895
                     /gene="rny"
                     /locus_tag="IXG20_000754"
     CDS             9339..10895
                     /gene="rny"
                     /locus_tag="IXG20_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ1608968.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(10974..11780)
                     /gene="proC"
                     /locus_tag="IXG20_000755"
     CDS             complement(10974..11780)
                     /gene="proC"
                     /locus_tag="IXG20_000755"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ1608969.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            11921..14494
                     /gene="mprF"
                     /locus_tag="IXG20_000756"
     CDS             11921..14494
                     /gene="mprF"
                     /locus_tag="IXG20_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1608970.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            14648..15499
                     /locus_tag="IXG20_000757"
     CDS             14648..15499
                     /locus_tag="IXG20_000757"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ1608971.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            15744..16682
                     /locus_tag="IXG20_000758"
     CDS             15744..16682
                     /locus_tag="IXG20_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ1608972.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            16705..17385
                     /locus_tag="IXG20_000759"
     CDS             16705..17385
                     /locus_tag="IXG20_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1608973.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            17358..18182
                     /locus_tag="IXG20_000760"
     CDS             17358..18182
                     /locus_tag="IXG20_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1608974.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            18349..19167
                     /gene="purR"
                     /locus_tag="IXG20_000761"
     CDS             18349..19167
                     /gene="purR"
                     /locus_tag="IXG20_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ1608975.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            19670..21043
                     /gene="glmU"
                     /locus_tag="IXG20_000762"
     CDS             19670..21043
                     /gene="glmU"
                     /locus_tag="IXG20_000762"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ1608976.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            21137..22108
                     /locus_tag="IXG20_000763"
     CDS             21137..22108
                     /locus_tag="IXG20_000763"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1608977.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      22114..22216
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22282..22851
                     /gene="thiT"
                     /locus_tag="IXG20_000764"
     CDS             22282..22851
                     /gene="thiT"
                     /locus_tag="IXG20_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ1608978.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(22932..23705)
                     /locus_tag="IXG20_000765"
     CDS             complement(22932..23705)
                     /locus_tag="IXG20_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608979.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            23971..25671
                     /locus_tag="IXG20_000766"
     CDS             23971..25671
                     /locus_tag="IXG20_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ1608980.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            25641..25859
                     /locus_tag="IXG20_000767"
     CDS             25641..25859
                     /locus_tag="IXG20_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608981.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            25879..26769
                     /locus_tag="IXG20_000768"
     CDS             25879..26769
                     /locus_tag="IXG20_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ1608982.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            26787..27071
                     /locus_tag="IXG20_000769"
     CDS             26787..27071
                     /locus_tag="IXG20_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608983.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            27558..28172
                     /gene="gmk"
                     /locus_tag="IXG20_000770"
     CDS             27558..28172
                     /gene="gmk"
                     /locus_tag="IXG20_000770"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ1608984.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            28177..28482
                     /locus_tag="IXG20_000771"
     CDS             28177..28482
                     /locus_tag="IXG20_000771"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1608985.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            28735..31143
                     /gene="priA"
                     /locus_tag="IXG20_000772"
     CDS             28735..31143
                     /gene="priA"
                     /locus_tag="IXG20_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ1608986.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            31158..31649
                     /gene="def"
                     /locus_tag="IXG20_000773"
     CDS             31158..31649
                     /gene="def"
                     /locus_tag="IXG20_000773"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1608987.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            31642..32580
                     /locus_tag="IXG20_000774"
     CDS             31642..32580
                     /locus_tag="IXG20_000774"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ1608988.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            32580..33938
                     /gene="rsmB"
                     /locus_tag="IXG20_000775"
     CDS             32580..33938
                     /gene="rsmB"
                     /locus_tag="IXG20_000775"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ1608989.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            33951..34691
                     /locus_tag="IXG20_000776"
     CDS             33951..34691
                     /locus_tag="IXG20_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ1608990.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            34688..36757
                     /gene="pknB"
                     /locus_tag="IXG20_000777"
     CDS             34688..36757
                     /gene="pknB"
                     /locus_tag="IXG20_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ1608991.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            36922..37821
                     /gene="rsgA"
                     /locus_tag="IXG20_000778"
     CDS             36922..37821
                     /gene="rsgA"
                     /locus_tag="IXG20_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ1608992.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            37834..38484
                     /locus_tag="IXG20_000779"
     CDS             37834..38484
                     /locus_tag="IXG20_000779"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1608993.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            38485..39123
                     /locus_tag="IXG20_000780"
     CDS             38485..39123
                     /locus_tag="IXG20_000780"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ1608994.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            39278..40132
                     /gene="mreC"
                     /locus_tag="IXG20_000781"
     CDS             39278..40132
                     /gene="mreC"
                     /locus_tag="IXG20_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ1608995.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            40136..40645
                     /gene="mreD"
                     /locus_tag="IXG20_000782"
     CDS             40136..40645
                     /gene="mreD"
                     /locus_tag="IXG20_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ1608996.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            40900..42438
                     /locus_tag="IXG20_000783"
     CDS             40900..42438
                     /locus_tag="IXG20_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1608997.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTVPESSATEE
                     SKTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
BASE COUNT        14006 a         7582 c         9187 g        11711 t
ORIGIN      
        1 attcgtaagg ttcgtttgct tttttgtgtg cgaatcggta tgctaagaat aaaaaagcag
       61 ggggcgtttg cttagtggaa tatcaggtaa agaaatttga agaattgtca acagcagagt
      121 tttacgcgat cgtcaaagaa cgaatcgcag tttttgttgt ggaacaacag tgtccttatc
      181 aagaagttga tgacattgat ggacaagctt ggcatacgtt ttttcaagat actgatggga
      241 agatccttgc ctacacgcga gtatatgaag aagcggagag tatccatttt ggacgtgtcc
      301 ttgttcatca ggacaaccgt aaagatggtt taggaagaaa gattgttcaa gaaacgatta
      361 acatgatcca gaaaaatttt ccagaaaaac caatcgtgat cggcgcacaa gaatacctac
      421 aagcttttta tgagtcgttt ggattcaagg caatctcaga tgtgtatttg gaagatgata
      481 ttccacatat cgacatgaaa aaggaacaag tagcaagtta agaatctaaa aaaagaaagc
      541 cgacactgaa aatcggcttt ctttttttta gattctttat tatttagaag tatcggaaag
      601 atttgtttgc tctactggat gtgtattgtc ttctgccact tgtttggcaa gaagatcgcg
      661 tatgtcacta agataatctt ctgcagtagg agtttcttcc acttccactt gctcttcttt
      721 ggagcgtaaa ttcttcgcac ggtttgccgc tttgacaact aagaataaga caaaaccagt
      781 gatcagaaat gtaatgatag cactaataac gttaccaaac gcaaatgtga cgccttttgt
      841 cggcgaccag tcaagaattg aaagtgctcc ttctaaatca gaagtacctg tgatcattgc
      901 gacaaaccaa cctattagtg gtgtgatcaa gccttgcacc acttggttta cgatgcttgt
      961 aaacgcacta ccaataacaa cgccgacagc aagatccaag acatctccac gcatgatgaa
     1021 ctctttgaat tctttgatca tgaatgaccc tcccttcttt tatttgtctt tagtatatcg
     1081 aaaaataaaa aataaaaaaa caatatacgc ttttagccgt ctgtttcttt tgataaaaaa
     1141 catctatgct atactataaa gtgtaacatc atttagaaaa aacggcgaat tgcctgaact
     1201 tataaatttc aagcagagga ataagtaaaa aaggcaggtt gacccattaa aagagtaagc
     1261 gggcaatcaa ctgtgaaaag gagcatataa atatggtgta tcaattggca gaaggcgtga
     1321 atcttcatgt cttaccaaca aaacaataca aaacgatccg gatttttatc cgttttactg
     1381 cgcgtctgca acaagaagtc atcacgaaac gttcacttct cagcagcatg ttggaaacaa
     1441 atagtttgaa ttatccggat cagacaaagt taagtgcgca acttgcagag ctttatggcg
     1501 caagctttgg attatcagtt agaaaaaaag gaaatttaca ttggctaaac gcagggatca
     1561 gttttgtgaa tggtgaatat gtcaatgatc caaacctttt ttctcaagcg gtagattttt
     1621 taaaagaggt tttattttat ccaaatatta aaaatcatca gtttgatcag ttgacgtttg
     1681 acttggaaaa gaacaatctg cgtctatatt tggaaagttt gaaggaagat aaacaaactt
     1741 ttgcttccta tgccttgcaa gaactttatt ttgaaaacag cccggaacaa aagatcccag
     1801 ggttgggcgt agtcgaagat ttggacaaga tcactgctag ttcgctagcg tcctactatc
     1861 aagaaatgat ggcaaatgat caaatcgaca tttttgttgt cggcgatgtt gatccggaca
     1921 aagcagctga agcagttggg caattaccat ttgagtcgcg ggaaacagtt tatccagatc
     1981 tgttttatac ccaacctcaa gtgaatatcg tgaaggaacg acaagtacgt gagccaatcg
     2041 ttcaagcaaa gctgaaccta gcttatcaga caaatgtgta ttacgatgaa ccagaacgtt
     2101 ttgcgctaat ggtctttaac ggtttatttg gcggttttcc tcactctaaa cttttcatga
     2161 atgtcagaga gaaagaaagt ctagcttatt atgcctctag tagcgtggat acgttccgag
     2221 gattcatgag tgtccaaacc ggtatcgatg aaaagaaccg aaatcaagta cttcatctga
     2281 ttcacgagca attagaaagt ttgcgaaatg gtgaaataac agagttggaa ctgtctcaga
     2341 caaaagcaat gctgagaaac caatatctgc tttctctaga ctctccgcaa gcggcaatcg
     2401 aagcttcttt tttggacagc tggctgccgg aaacaaaatt atctgatgaa gaatggttga
     2461 aacgtatgga gagcgtaacg atcaaagaga tacaacaagt agctgagcaa atcgaattgc
     2521 aggcaatctt ctttttagca ggaggaaatg caaatgaata aaacagaata tgagcaaatc
     2581 aatgaaacac tttatcatga agtactgcca aacggtttga ccgtgtattt gttgcctaaa
     2641 aatgactatc acaagacata tggtttgttc agtacgaatt atggttcgat cgataacgaa
     2701 tttattccct atggtgaaaa agaaaaggtg aaagttccag atggaattgc ccatttttta
     2761 gaacataagt tgtttgaaaa agaagatggt gatgtctttc aattatttgg gaaacaagga
     2821 gcttccgcca atgctttcac aagttttacg aaaaccagct atctgttttc tacgacggat
     2881 caggtagaaa agaatctgac gactctgatc gattttgtac aagcacctta cttcacagaa
     2941 gaaactgtaa acaaggaaaa aggaatcatc ggtcaggaaa tccaaatgta tgaagacgac
     3001 cctaactggc ggatgttttt tggcatacta aataacttgt atcctacaca cccattgcat
     3061 atcgatattg cgggcacggt tgaaagtatc gacaaaatca cagctcaaga cttgtatacg
     3121 tgctatcgga cgttttatca gccaagcaat atggttttat ttgtagttgg taagatggag
     3181 cctgagaaat tgatgaaact tattcgggaa aaccaagaag caaaaaattt ccctccaaag
     3241 caagaaattg tacgttattt ccctgaaaac acaaaagaaa tcatcaaaca aagtgcattg
     3301 gaagcagcca ttacacgaga taaatttgta cttggtatta aaggattaga cacattgccg
     3361 caagaaggaa cagaactgtt acgctataaa acagcaatca atctgttgtt ccaaatgatt
     3421 ttaggcaata cttctcgaaa ctatctagcg atgtacaatc aaggaattat cgatgacagc
     3481 tttggttttg aattcagctt ggatcgtgaa tttcactttg cggacttcag cggagatacg
     3541 gacgaaccag agaaagcagc agaaaaagtc aaagaaatca ttcttggatt cgctgatgat
     3601 ccggaagtgt cagaaacaaa tctcgatttg ctgaagaaga aaatgcttgg acaatatttc
     3661 caatcattaa attcgatcga atatattgcc aatcaattta cgcaaagttt atttggcgat
     3721 cgaactttat ttgatcttcc tgaaatcatc gattcgatcc aaatgaaaga cgtactggca
     3781 gctggggaag catttataag tgaagaggcc ttcagcgagt tttacatgag accaaaataa
     3841 accagtattt tatgaggaaa agtcttaaat ttcgttttag acttgtagta ggaacctcct
     3901 ccaaaatcag cttttatggt attcttagtt gtgttggaaa aattagtgag aaaataaaac
     3961 aaatcatgct ctcttttaat caacttatat taaaataaaa tcaaacgaag taatcaaaga
     4021 ggttgtgaca gaagtggtca gcttcaagaa ataagaagga atttacgaaa attgcttctc
     4081 aaatttttgt gatatttcgg cttattgccg aagaagctac ttctggaaca ccgtgtacca
     4141 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaagta aaaacagctt
     4201 cttaatgttt ttaacttggt tggacttatt accgcagatg caagcttttg aacaccgttt
     4261 aaggaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacat acggaaaaat
     4321 atctccttat attttgccgg tttttgagct aataccgagg aacagctgat tgaacaccgt
     4381 ttaagaaaga ggtcgtgagc ctgccgttta actccgagtc acaaggaaca agttcaaaaa
     4441 acagcttctt atatttattg gatattgtga cttgtgaccg aggagttggc aggagaacaa
     4501 tgtttattcg gaaaaaagga gctgtgacat gacttttgtc acagcaccca aacataacgt
     4561 ctttcgttgc aacagcagat gcttcggaga atcaaacaga ctggagaaga tgaacgagtg
     4621 gcttttgaaa ccatcggaga aaaattacgg caagcacgcc tgaataaaaa aatatcttta
     4681 gatgaattac agcagattac aaaaattcaa aaacgttatt tggaagcaat cgacaatgat
     4741 gattttgacc aattgcctgg taaattttac gtgcgggcat ttattcggca atacgctgaa
     4801 gctgtaggag aagatggaga ccatttagtc gatgtttttg atgggaaaag caaattgaca
     4861 ggatcggtcc ctgaacgtcc gcagcctgaa acagtaagta cttctagaaa agcaatgcat
     4921 caggaagaat cgcatccaag tcgcttttgg acaagtcttc ctgtcatttt attaggattg
     4981 gttgctttgg cgattgtcgt tgtcgtgggc tatatgactt ggcaagatcg tcaagctgat
     5041 ccaataatcg gagacacagc ttcttcgctt aaagtagaag gatcagtcga ggagtcctct
     5101 tcctcagagc aaagcacgca gaccaaagag tcttctacag tagaatcaac aacaaaatca
     5161 agtgaagaaa agaaaatggc aatagctaca ggagaagatt ctggcagtgc catcgcagta
     5221 aatatcacag acgctaccaa gcctgtaacc cttgaattta ctgctgtcaa tcgtgtatgg
     5281 ttaggtgtgc tagtagacaa tgcctatgtt tatcaaggga cactggcagc aaatgaaaca
     5341 cagagtaccg tgttaccgga aacagcaaca aatgcgacca tcacgattgg tgcagcaagc
     5401 aatgcaacga tcaaagcgaa tggagaatcc gtaccagtta atccagggga aaacaatcag
     5461 agtccaaaaa atgtgaacct tactcttcaa tacgcagaat gatcgtataa attagtttac
     5521 gcatctgttt acttgtaggt ataaaaaaga aggatcgtga caaatctgtg tcgcggtcct
     5581 tcttcgttaa ttcaaaaaga tcaatttcaa taaaaatact cctttgaata aattcatttg
     5641 gaaaagtaaa taatatcggt ggaaacttcg ctgatagtca cgtagaatag ggaagaaagg
     5701 agtcgatagt gtgaatttac caaataaatt aactgtactt agaatttgta tgatcccgat
     5761 tttcattttg gttgtaggtt tacctttaga ttggggacaa tttaacgtag gagacaccac
     5821 actggctgtc acacagctag taggtgcagt tatttttgct ttagctagta tcacagactg
     5881 gttagatgga aaaattgccc gtgcgcgagg ccttgtcact aattttggca agtttgcgga
     5941 cccgttggca gataaaatgc tagttatgac tgcttttatc atgttggttg accaaggaaa
     6001 ggcccctggt tgggtagtag cgatcattgt ctgtcgtgaa ttagccgtca caggcttgcg
     6061 tttactactt gtcgaacatg gagaagtcat ggctgcagct tggccgggaa aagtcaagac
     6121 ggctacacaa atggttgcta ttatcttgct gtttatcaat aatatcccat tcagcctgat
     6181 cggtattcca gtagatcaaa tcatgcttta tgcttgtctg atctttacta tctattcagg
     6241 tgtggattac ttcatcaaaa acagcggtgt gttcaaagga tcaatgtaag tatcgtttag
     6301 tggagaggtt gtgagcctgc cgtctaactc tgagtcacaa gggataattt caaaaaacag
     6361 cttttcatgt tttatgtata ttgtgactta tgaccgaggt tttggcagga gaacactgtt
     6421 tatttggaac aaggagctgt gacaagattt ttgtcacagc tccttgccct ctttttaaat
     6481 aaaaaaatat aaaaatacag cgtatactat ataagggagc gggaagtaat gaaagcagaa
     6541 ataatagctg tcggaacaga acttttacta ggacaagtcg tgaatacgaa tgccactttc
     6601 ttgtcagaac agctagctga tctaggtatc gaagtttatt atcaaacagt tgtaggagat
     6661 aatcagcagc gtttagaaga gttgattgct ctagctgaaa caagaagtga actcatactc
     6721 ttatgtggcg gattaggacc gacagaagat gacctgacaa aagaagcaac tgcggctcac
     6781 ctcggtaagt ctctcataca aaatacggaa ggatacaaaa aattattggc atattttgaa
     6841 acaacccatc gaaaaatgac caaaaacaat ctgcaacagt cccagattat cgaaggcggg
     6901 gttcctctac caaatcgtac agggcttgct ttagggactt tttatcaaac agatacacat
     6961 gcgtatatcc ttcttcccgg tccgcctaat gagttgaaac caatgtttgt ggaacaagta
     7021 cgtcctttac tagaggaacg ttttccttct gaagaaaaac taatttctaa agttttgcgc
     7081 ttttatggga ttggtgaatc gcgactggtc actgagctaa aagatctgat tgagacacag
     7141 atcaatccaa cgatcgcacc atatgccaaa ccaaatgaag tgacattacg actgacagtg
     7201 aaaacaaatg atgttcaggc agggaatcaa gctcttttag cattagaaga aaaaatccaa
     7261 gaacgagtag gggaatattt ttatggatac ggcgatgata attcgctagc aaaagtcgta
     7321 gtggaactac tgaaagaaaa caagcagact gtaacagcag cggaaagttt gacagccggc
     7381 gccttccaag ccgcattagg cgacattgct ggtgtatctg aagtatttcc aggcggattc
     7441 gttacctact cgctccaaac aaaagctggt tttctggaga ttgatccgga gctattagca
     7501 gaatatggaa cagtcagcaa agaatgtgtg gagcagatgg ccattcaagc acgtgagata
     7561 gcaagaacag attacagtgt agctttcacc ggagtggctg gaccggacac gttagaaggt
     7621 caatctgcag gaacagtatg gatcgctatt tcatctaaag acggcgtggt ttcggaaaaa
     7681 tatcactttg ccagagatcg tgctgctatc agatataatg cggttatgca aggatttgat
     7741 cttttgagaa aacagctttt aagaaaatga taaatgcgaa tatttgttcg gttttttctt
     7801 gctttttttc actaaaaaag atatgataag gataatgaaa tgttgctctg atttgtaaat
     7861 ataaggagga ttatcgtatt ggcagatgat cgtaaagcag ccttagatgc tgcactgaaa
     7921 aaaattgaaa agagctatgg taaaggctct attatgaaat tgggcgaaaa aatcgaccaa
     7981 caaatctcta caattccaag tggctctctt gcgttagatg tcgctttagg tgtcggcggt
     8041 tacccccgtg gacgtatcat cgaagtatat ggacctgaaa gttcaggtaa aacaacagtt
     8101 gcactacacg ctattgcaga agtacaaaaa aatggcggaa cggccgcttt cattgatgct
     8161 gagcatgcgt tagatccgca atatgcacaa aaattaggtg tgaatatcga tgaactactt
     8221 ctttcacagc ctgacacagg agaacaaggt ctagagatcg ctgatgcttt agtatcaagt
     8281 ggggctgtag atatcgtagt agtcgattca gttgctgctt tagttccacg agcagaaatc
     8341 gacggcgaaa tgggtgactc acatgtcggg ttacaagcac gtttgatgtc tcaagcattg
     8401 cgtaaactct ctggttcgat caacaaaaca aaaacaatcg ctattttcat caaccaaatc
     8461 cgtgaaaaag taggcgtgat gtttgggaat ccagaaatca ctcctggagg acgcgcattg
     8521 aaattctacg caacgatccg tttggaagta cgtcgtgcag aacaattgaa acaaggtaca
     8581 gatattgttg gtaaccgtac aaagatcaaa gtcgtaaaaa acaaagtagc acctcctttc
     8641 aaaatcgctg aagtagatgt catgtatggt ttagggatct cacaagaagg agaacttcta
     8701 gacatggcag tagaaaaaga cattgttgat aaaagcggtg cttggtattc ttataaagaa
     8761 gatcggattg gtcaaggacg ggaaaacgct aaaatctata tggctaatca tcctgaaatg
     8821 atggcagaag tttctgctct agttagagca gcttatggta ttggtgaaga agtcgctgtc
     8881 ccagaagatg aaaaaggaca agaagaattg cctttagtag aagaataaaa attatgataa
     8941 acaaaaacgg ctggtctgaa gagtaggtac tctccttgac ctgccgtttt tttattgaat
     9001 aaagaaatcg tgagttattt aattatacga gtgggtacat aaaataaaaa tatctgttca
     9061 tctacaaaga ataatttaat agaaaagttt ctcaatttaa aggtttacct atatgaaact
     9121 agtgtcattt attttaaaaa ccatcgttta atgaataagt agaaagccgt ttctttgttt
     9181 atttaaagtt gacactacgg ggtacaaaca ttagaataaa gttgtgcatg ttttttgtac
     9241 acatatatgc gaagatgcat attgacgagt ttagaataat ttatcataga agctgtatag
     9301 atgataaaaa tgaaaaagat agtatggagg tggattttat ggtattcaac attctcctcg
     9361 ctatcatcgg tttaattgtc ggtcttggtt tagggtttat ggtcgcaaag tcacgtcacg
     9421 agaaagaaat agctggtgca caatcatcag ctgctggaat catcgatagt gcaaaaaaag
     9481 aagcagaaac tctaaaaaaa gaagcattgt tagaagctaa ggaagaaaat cagaaatacc
     9541 gttctgaagt tgaaagtgag ctgagagaaa gtaagttaga attaaaatct caagagaatc
     9601 gtttgattca acgcgaacaa acgctgaatc gtaaagatga ctcgttagaa aaacgcgaga
     9661 actcacttga aagtaaagaa gaagcactta gttcaaaaca gcaattaatt gaagagcgag
     9721 aaaaagacgt tgaaaaaatg attgaagatc aacaaaaaga actagaaaaa attgctggtt
     9781 tatcaaaaga ggacgcacga gatgtcatca tgaaatcgac tgaagaagag ctgaatcatg
     9841 agttaactct aatggtaaaa gaatctgaac agcgtgccaa agaagaagca gatcgcaaag
     9901 cgaaaaactt attatcatta gcgattcaac gctgcgcagc agatcaagtt tcagaaacga
     9961 ctgtttcagt cgttaccctt ccgaatgatg aaatgaaagg tcgtatcatt ggacgagaag
    10021 gtcgtaatat tcgaacgctt gagacgctga ctggtatcga tttaatcatc gatgatacac
    10081 cggaagcagt tgttctttcc ggattcgatc caatccgaag agaaatcgca cggatgacct
    10141 tggaaaaact gatacaagat ggacgaatcc atccagcgcg tatcgaagaa atggtcgaga
    10201 aatctcgtaa agaaatggat gaacgtattc gcgaatacgg ggaagaggca gcgtttgaag
    10261 tcggtgctca tacacttcat ccagatctaa tcaagatcat cggacgtctg cgattcagaa
    10321 ctagttatgg acaaaacgtc ttgaagcatt cggtcgaggt tgcaaaatta gctggtatct
    10381 tagcagcaga gcttggcgaa gatatccaat tagcaaaacg tgccgggttg ctccatgata
    10441 ttgggaaagc acttgaccat gagattgaag gatctcacgt tgagatcggt gcggaattag
    10501 ctgccaagta caaagaaaac ccagtagtaa tcaacgcgat cgcttctcat catggagatg
    10561 tggaagctac ttctgttatc tctgtccttg tagctgcagc agatgcgctc tcagcagcaa
    10621 gacctggtgc gcgaagtgaa tcattagaaa actacatccg tcgtttaaga agtctggaaa
    10681 atatttcgaa cagcttcgcc ggtgtagaat caagttacgc agttcaagct ggacgcgaag
    10741 ttcgggtaat ggtcaagcca gaagaaatct cggatttaga cgctgttcgt ttagtacgtg
    10801 atattcgtaa acgaatcgaa gaagaactgg attatcctgg acacatcaaa gtaatcgtta
    10861 ttcgggaaac tcgtgcaact gattatgcta aataataaaa aatacaaaag aagagccgcg
    10921 gcaaaagcca cagctcttct ttttttgtaa ggtacgaaaa aacattgcta tcattatttt
    10981 ttattcaaca tctcaagatc tttctcgatc gtcgtattga ttcctttgat gattgctggg
    11041 atgaatcctg cttcttctaa ggcaactaca cctgcaacag ttgtaccgcc aggagaagaa
    11101 acttggtcta caagttccca tggattttta tcacttgaaa ggaatttttg acaagaaccc
    11161 ataattgctt ttgcagcaat ctcagtggct gtctgtttgg acaaaccatt cagtacacca
    11221 gtccgtccca tcgcatcgat cagcatgtaa acgaatgcgg gtgagctgcc agcaaggcca
    11281 atgaatgtac tgaaatctga ttctgctaaa ggatagacgc tgccgattgc ttcaaaaatc
    11341 atcgtagctt gctgaagctg ttcctccgtg gcagcttcat ttttgcaaat cgcagaaacg
    11401 cctgctccta cgctgacatt gacattcggc atcacacgta cgatggaggc ctgaggatct
    11461 tcaagcgctg tttctaaatc tgttaatgat tttcctgaag caatagaaat gaataagact
    11521 ttctggctat attctcttag ttcttccaat actttttcaa tgatttttgg tttcacggct
    11581 aaaagaacga tatctgattg tgatgcaact tcctgattac ttttgcatgc atggacgcca
    11641 gtttcagcag caaatttttg tgcattttca aaatgtccac tggaaacgaa cacctcttct
    11701 ttcgagactg ttcctttttt taataaacca tcaataatag cttgtgccat attccctgtg
    11761 ccgataaatc ctactttcat gttatcctct cctttagcat taggatatct ttctttttct
    11821 atcatcgcaa gcaaagaaca ggtctaaagt ggaaaaatta aaataaataa gataacattg
    11881 gggtatgaca caaaagtata caagttagga atgggaaaat gtgaaaaata aacgatactt
    11941 gatgctgcaa tggataaagg aacatagtct gattttaaaa ctgatttttt tcggctccgt
    12001 gttagtcttt gtggcaaatc aagtcgcaaa tattgctaat ggcatgagtt ggcaagatat
    12061 ttggcaaagg atgggacaac aaagccgcat gacgatcctg ctcatgattc tggctggact
    12121 gctgggtgtc actccgatgt tgttgtatga ttgggtgacg atcagagtct tagaaaaaca
    12181 aggaaaacca aaaatggaaa gaaaagagtt tcttcttgct gcgtgggtaa caaacacgat
    12241 caacaatctc gccggttttg gtggtgtggt aggagcaagt ctgcgggcta gtttttatgg
    12301 aaagcagaca aatagaaaaa tggttttagc cactgtatca aaagtcgcac tatttatgct
    12361 gactggtttg tctgtatggt cgtttcttac atttattgat gtctttttta ttcagtcaga
    12421 aagtattttt cgaagctatt ggatctggct tttcggcggt agttttttag caccagtcat
    12481 tttctttttt gcttatctaa agagaaagac attgttcttc gagttttatc caaaaggctt
    12541 gcttggtctg ctactggcat cctttggaca atggacggga gcccttgcgg tatttttgat
    12601 catcggcaag ttgatgagaa tagatatttc gatgctttcg atctatccta tgttcatgat
    12661 tgctacattg ataggaatgc tgacgatggt cccaggtgga atggggactt ttgacgtttt
    12721 gatgatctta ggaatgagtc aattgtctgt tcggcaagat gtagcagtag tttggctatt
    12781 atattatcgg ttgttttatt atgttctacc ttttatgacg ggacttctat tgttcatcac
    12841 acatacagga gtcaaaatca atcgattttt agataacatt ccgcgactgt ttttccaaaa
    12901 ggttgcgcat atcgtgctcg taagtgctgt ctattttgcg gggatcatga tggtacttct
    12961 atcaacaatc actaatctgt ctgcaatcag tcggctattt caagtattat tgccgttctc
    13021 attcaatttt ttagatcaga cagtgaatct attggttggc tttttattac tcggccttgc
    13081 tagagcactt tcagtaaaag tcaaaaaggc atttctgcct acgattgctt tactatcttt
    13141 tggtatttta aatacaatca cacgtacgtt atcttggcag ttgatattgg tatatgtctt
    13201 gattctgtta gctgtgtggt tatccagaaa agaattttat cgaacaaaat tcgtttattc
    13261 ttggggagct ataatctttg atgctagctt gttcggcttt ttgtttatcg tttacgcaat
    13321 tgcaggctat cacagcggac gctttttagt cggtcgaata acaggaaatc attttatttt
    13381 gtttccttca gatgatgtgt ggttctcagg gcttattggt ttgggcatct cattgctagc
    13441 tttagtggcg ctttatcaat atttagctga agatactacg aagcttggca gtgagtacga
    13501 aaaagaccgt tttgaccgtt tattggaaac atacggaggc acagctacaa gccatcagct
    13561 tcgcttgtca ggttacagtt atttttatta ccaagaagac gaccaagatc aagtcgtttt
    13621 tggttatcag atcaaaggaa acaaatgctt tgttttagga gatccagttg gcaatcctaa
    13681 aatgatgcgg gcagcaacga atcagtttat gaaacaagca gattgtttag gctatcagct
    13741 tgcgttttat aagatatcag aaagctacgt agtgatgctt catgaattgg gttttcactt
    13801 cacgaaagta ggtgaagcag ggattgttga tctagcaaca gaagacttgt ctcggctgag
    13861 tcagcaactt gaatatcaac gtttgatgaa agaaggatac cattttactt ggtacgatca
    13921 actgccggaa gaattacatc aagctgtaaa tgaagtttca caaaaatggc ttcaaggtgc
    13981 acgagaaaaa tacttctctg tgggacgatt tgataaatca tatgtattat ctagcggtgt
    14041 cggtattgtc cgtaaagatc aaaaagtcgt tggcttcatc accgctcagc cgatgacgaa
    14101 gaagcaggcg ggatatgatt tgttgcgggt cttacctaaa gaatctgagg aattagctga
    14161 ctatctgtta gccaatctgt ttattgtata cgagaaaatg ggctactcgg aagccaatct
    14221 aggcttagct ccattagcta atgttggaga aacagatttt tctttctttc aagaaaaagt
    14281 catgcatatc gtatataatt atggtaattt tttctattcc ttccagtctg cttatgaaaa
    14341 gaaattaaaa tatgtcgatc attgggaagg acgttatttt gcttatagga aaggaagtaa
    14401 tttcggattt gctaccatgc agctactcag tctgatagga aaagaaaaag gaaatgttcc
    14461 gagtttggca gaagaagtgc tcacagaatt ataagaatca ctagaacttg tttggattat
    14521 gaagagaaaa ttctgtgaaa gctgttgaaa cgatgacaaa atcaaccact aggcagacat
    14581 aagctatact tttatttttc cctatgatag aatacttgcg acttagactt ttcgaatggg
    14641 tgagaggatg gaattgattg aaaaagcgcc agccaagata aatttaggac tggatgtatt
    14701 atatagacgg caagatgggt atcatgagtt ggaaatggtg atgtcgagcg tagacttggc
    14761 agatcatcta ttttttgaag aaataacaga agacaaaatc attgtagaaa caaataaagc
    14821 atttttaccg gtagatgaac ggaacaatgt ttatcaagct gctttattgc taaaaaataa
    14881 atacaatatc aaaaccggtg tcaagatctt tgttaagaag aatattccag tagcagctgg
    14941 attaggcgga ggaagtacag actgtgcagc tgctctaaga gggatcaacc aattatggaa
    15001 tctgggctgt tcgttagaag aactggcaga aatcggtttg gaagtcggta cagatgttcc
    15061 ttactgtgtc tacggaagta cggcatttgt cggtggcaaa ggggaagaaa tccaagtatt
    15121 accttctatg ccacagtgct gggttgtgtt ggtcaaacca agaatgagtg tctctacacg
    15181 tacagtgttt agagaagtag atacgcaagc catccaacat caagacatcc acggacttgc
    15241 tgaagccatc cgagagcaag attatcagca gatgatcaaa aaaatgggaa acagtttaga
    15301 agatataacg ggaaagcggc atccagtcat ccatcaaatc aaagagcgta tgatcaagta
    15361 cggagcagat gctgcattga tgagtggaag cggaccaacc gtttttgcct tgtgtcaaca
    15421 ttattcaaga gcgcagagag tatataatgg gttaaaaggt ttttgtgagg aagtttattt
    15481 agtacgaacc ctgcaataaa atataagaag taaaataggt gatgaaaaac tgaggaatcc
    15541 taaaatggta ttcttcagtt ttttattgta gtgaaatggc tagatatgag cagcaactca
    15601 tccattttac gatttaatca agaatataca tgcttaaatc agaaattgat ggatttctat
    15661 tgactgtgtt tttttatttt gatacattag ttcacgttaa taaatcgtaa aattccgatt
    15721 tgttcattgg aaggagcata taaatgaaaa aatatctttt gacagctacg gtaatgatag
    15781 gtgctttact gtttgcagca tgtggaaata cgaacaagga agcagacaaa aaagaggatt
    15841 tgacgatcgt tacgactttt tatccgatgt atgattttac aaaagagatc gtaggggatg
    15901 aaggaaacgt gaaacttttg atccctgctg gaacggagcc gcatgatttc gaaccaagtg
    15961 caaaagaaag agcagaaatt tccgatgcag atgttttcgt gtataatagt tcagacatgg
    16021 aattttttgt ggattcgtta aaggattcag tggatagcaa acagaccttg atgatcgaag
    16081 cagctaaagg aatcgaccgt ttagaatcgc aagaagcaga cgaacatgaa gaatcagaag
    16141 aagggcatgg tcattcacat gaatacgatc cacatgtttg gctcgatccg gtattagcta
    16201 taaaagaagt cagaacgatt gctgaagaat taggcgaaaa atatccagat aaaaaggaaa
    16261 cttttacgaa gaatgccgat gcttatatca aaaagcttga agcactggat caaaaatact
    16321 ccgatgagct aaaagatgca acaaaccgca catttgtcac tcagcatgct gcttttgctt
    16381 atttagcgaa tcaatacgac ttaaaacaag tagcgatttc gggcgtttca ccagatcagg
    16441 aacccacacc aagtcgctta gctgagctaa aagaatttgt gaagaaaaac aacatcaaag
    16501 tgatttattt tgaagaaaat gcctcttcga aggtagctga gacattgtcg aatgaaacag
    16561 gagtgaagct agaagtgttg aatcctttag aaagcttgac taatgaacaa atcaaagcag
    16621 gcgaaaatta tatttctgtc atggaaaaaa atctgaaagc tttaaaagaa agtatcaatt
    16681 aagttttaga atggaggttg taaggtgcgt tatatcgaaa tatctgattt gaccttttac
    16741 tatgatgatg aacctgtact agaagatgtc tcttatcatg tagatgcggg tgaatttgtc
    16801 attttaactg gagaaaatgg agcagccaag tcaaccttgg tgaaagcatc gttaggatta
    16861 ctgaaacctt caaaaggaat gataaagatt gctgagaaaa atcaagaagg aaaaaaatta
    16921 agtatcggtt atattcctca acaagttgca tctttcaatg caggttttcc tagtacggtg
    16981 atcgaattag tccgttcagg aagatatcca agaggccgct ggttcaagcg tctctctgaa
    17041 aaagatcatc aacatgtaca gcgtgcttta gaagctgtag ggatgtggga tatgcgaaat
    17101 cggcgaatag gtgaattgtc tggcggacaa aagcagcgaa tcagtttagc gcgtattttt
    17161 gctactgacc ctgacttatt tgttcttgat gaaccaacaa caggtatgga tgaagaatcc
    17221 agaaatgaat tttatcgtct tctccgacat agtgcgcatg tccatgggaa atcagttcta
    17281 atgatcaccc atgatcatga agatatcaaa cagtatgcgg acagacagat ccgactggtg
    17341 agaaaggagg attctcaatg gcgttgcttt catatgaatt catgagaaga gcgtttcttg
    17401 ccgctatctt cattgccgga atcgccccga tgctaggtgt gtttttagtg atcagaagac
    17461 aatcgctgat ggctgatacg ctttctcatg tctctttagc aggggtagcg ttaggattct
    17521 ttttgaacct taatcctaca cttacgacca tgctagtcgt tgtattagca gctgtgatat
    17581 tggagtatct gcgaacgatc taccgaacct attcagaaat ctccattgcc attttaatgg
    17641 ctggtggttt ggcgctagcg ttagtattga tgaatttgag cggagggaat tcagcaacaa
    17701 gtatccaatc ctatctgttt ggttcgatcg tgaccattac atgggagcaa gtcatctttt
    17761 taggaatctt attcgttgcc atcggtatat tgttcttttt attcaaacgg ccgatgtatg
    17821 ttctgacttt tgatgaagat accgcccacg tggatgggct acccgttcat ttgatgtcga
    17881 tgatgttcaa tgtgattact ggtgtggcta ttgctgtgat gatcccgatt gccggtgcac
    17941 ttttgatatc cgcaatcatg gttcttcccg cagcaatcgg tatgcgatta ggcaaaagct
    18001 tcaatgtcgt gattgctatc agtgtttgtg taggatttct agggatgctg tcaggactaa
    18061 ctagctcgtt ttatctagat acacctccag gagcaacaat cacattagtc tttattgctt
    18121 tgttcctgat tgtaaatatc atcaaacgaa tattggttgc cttcagaaga aaaaataact
    18181 aaattataaa gaaaagaagc cttataaaaa cgaaaaattt tttataggct tctttttatt
    18241 ttaataaata ccgaactatt ttcttatttt ttcataaaat gctcgaattt ttgaagaaaa
    18301 acttatataa tgtaaaacga taaaatttct taaaaggagt tggaataggt gaaagtacgt
    18361 cgcagtgaac gtctagttga catgacacag tatttattga atcacccgca tgaattgatt
    18421 tccttaacaa gctttgccga acgttatgaa tctgctaaat cttctatcag tgaagatttg
    18481 ggaatcatca agaaaacatt taaagaacga gggtatggaa ccttggaaac aatcccgggt
    18541 gctgcaggtg gtgtattgtt catccctgaa atttcatatg aagaggcgaa aaaatatatt
    18601 gaatctctag cagaacgtct atcagaacaa gaccgtctgc ttccaggagg atatgtttat
    18661 ctctctgatt tgttaggaga acctgaactg ctgcgtcaag ttggaagaat cattgcttct
    18721 aaataccttg gtgaaaaaat cgatgctgta atgactgtag ccacaaaagg cgtacctatt
    18781 gcccaagctg tgtcctatta tctgaatgcg ccattcgtta ttgtacgccg ggactcaaaa
    18841 ataacagaag gatcaacagt aagtgtcaac tatgtgtctg gatcttctga aagaattgaa
    18901 aagatggaac tgtctaagag aagtttgaaa cgtggttcac gtgtccttgt agtggatgat
    18961 ttcatgaaag gcggcggaac agtcaacgga atgaagagct tgattgaaga attcgaatca
    19021 gaacttgttg gcatcactgt atttgccgaa tcaaaattca acggtcatcg cgccatcgat
    19081 gattatacgt cactgcttta tgtcaaagat gtagatactc aaacgaaaaa tattacggtt
    19141 gtgccaggaa attatttttc agaataagag gttgtgaaaa agccgcttag tttcgagtag
    19201 taagaaaaat tttgtgaaaa tggctttcca cattttttca taaatttggc ttaataccga
    19261 gaaactggct tttgaacacc gtttatctag aggttgtgaa atcagcgtcc tgtcctgagt
    19321 attagctcaa taaacggaaa aattgctcct catattttgc cgtttattga gctaataccg
    19381 aaggtcagct gattgaaaaa cgtttaatga agaggttgta agcctaccgt ttaactctaa
    19441 gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt atgagtagtg tgacttgtga
    19501 ccgaggagtt ggcaggagag caccggttac caaatagata ggcccgttat ggtctatcta
    19561 tttttttatc cagaaaacgc tgggataaaa gaaaatcact tgttttgttg aaaaaatggg
    19621 tatactggga taaactgaaa tcagcaaact atatttagga gtgacgcagt tggacgcacg
    19681 ttatgcaatt attttggcgg cagggaaagg tacccgcatg aagtcaaaat tatataaagt
    19741 tttacatccc gtttctggta aacctatggt agaacatatc attaatcggg tcagtgaaac
    19801 gaaaccagat gaagtgatta cgatcgttgg acacggagca gaacaagtta aagcacaact
    19861 aggagaacgc agtaaatatg cgttacaagc agaacaattg ggaacaggac atgctgtttt
    19921 gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg ttagttatca gtggcgatac
    19981 accattattg actactgaaa cgctgaacaa tctttttgaa tatcaccaag gaaaaaatgc
    20041 aagtgcaacg atcttgacgg cacaagcaga aaacccaaca ggatatggac gaatcatccg
    20101 tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa gatgcaacac cagaagaagc
    20161 tcttgtacaa gaaatcaata caggaactta ctgttttgac aacgaagctt tgttcgatgc
    20221 gttaagcaaa gttggaacaa acaatgctca aggtgaatac tatctaacag atatcatcga
    20281 aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa acggatgatt ttgaagaatc
    20341 aatgggagtc aatgatcgga tcgcgttagc taaagcaaat gagatcatga gaaagcgaat
    20401 taaccagatg catatggtca atggcgtaag ctttgtcgat tcagcaacga catacatcga
    20461 tgcaggcgta gaaatcggtc cagacacatt gatcgaagca ggtgtacaaa tccaaggaaa
    20521 tacagtcatt ggttctgatt gtgtcatcgg gtcacattca aaaatcgtag acagtaggat
    20581 tgaggatcac gtcgtcattg aaaactcagt cattgaaagc agccatgtca agaaacatgc
    20641 agatgtggga ccttatgcac atttacgacc aaaagctgaa attggcgaaa acgtccatat
    20701 cggaaacttt gtggaagtga aaaatgctca aatcggcaaa ggaactaaag tcggtcactt
    20761 gacttatgtt ggtgatgcta cgctgggaga agaaatcaat gtcggatgtg gtgttgtctt
    20821 cgtcaactat gatggaaaga acaaacatca tacgactgtc ggcgaccaca gctttattgg
    20881 ttctagcacg aatatcatcg gacctgtcga ggtagctaaa aacagttcga ttgctgccgg
    20941 gtcaacaatt acagacaata ttccagaata tgcgctggca attgcgagag caagacaagt
    21001 caataaagaa ggatatgcaa aaaaactccc ttatctaaac taagactttt gcaaaatcac
    21061 ttgatttatt taaaaaatat gactactatt tattaggtag aattctagag ttgtgaaaaa
    21121 gaaagtggag gtccccatgt caaaacatta ctttgaccca agattaaaaa tttttgcgtt
    21181 aaattccaat cgcccattag cagagaaaat tgcagcagca gtcggcgtcg agttaggaaa
    21241 atcatctgtt actcaattta gtgatggaga aattcaagta aacattgaag aaagtattcg
    21301 tggatcgcat gtctatgtta ttcaatcaac gagcagccca gtcaatgata acttgatgga
    21361 gttgctgatc atgattgacg cactgaaacg agcaagtgca aaaacaatca acgtagtcat
    21421 gccttattat ggctatgcgc gtcaagaccg taaagcacgt gcacgggaac caatcacagc
    21481 gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca agaatgctga ctttggacct
    21541 tcatgcagtc caaatccaag gtttctttga tattccagtc gatcatttga tgggtgcgcc
    21601 acttattgcc aattatttct tagaaaaagg aatcaaaggc gatgatgttg tcgttgtttc
    21661 accagaccat ggtggtgtca cacgggcacg taaattagcg gaatttttga aatctccgat
    21721 tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca gaagtaatga atatcattgg
    21781 acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg atcgatacag caggtacgat
    21841 cacattggct gccaatgctt tgaaagaagc tggtgcaact agtgtgtatg cttcatgtac
    21901 acatccagta ttatccggcc ctgctttaca aagaatagaa gattcagcta tcgagcgtct
    21961 agttgttaca gactcgatct atcttccaga agatcgtaaa atcgacaaaa tcgatgaagt
    22021 aagtgttggc ggactaatgg gagatgcaat caaacgtatc catgaaaaca aaccagttag
    22081 tccattgttt gaaacaaaaa ataaataata aataaactca caaaggggag tccaattggg
    22141 ctgagattga atctatttct aaacccttcg tacctgtatc ggttatgcga gcgtaggaat
    22201 tgtgaataaa cagctgtttg actattttcg aaagctgctt cctcctttgt gactttttta
    22261 ttcaaaggag gaagtttttt tatgggaaaa aatagagttt gggtagaagg aacgattgtt
    22321 gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga tcggcagcag tttttctatt
    22381 tcattagggc agattccatt gactcttttt gcgttaagaa gagggacaaa ggcagggatg
    22441 ctggcaggtt tcatttgggg catcctacat tttccgttgg gacaagttta ttatttaagt
    22501 gtcgttcaag tgttgattga gtacccgatt gcctatactt ttgcaggcgc agcagggctc
    22561 atggcttcaa atgtccaaaa aggctatgta gacgataata cgaaacaaat tagaaaaagc
    22621 atcgtgtttg gtgcattgat tggcgcactt tccagatatt tttggcattt tattgcagga
    22681 gtggttttct ggggagcgta tgcactatgg gggatgaatt cttggctctt ttctttcgta
    22741 atgaatggtg ccagcgggtt gagtacagct attgtaacga tcatcgtttt attagggatc
    22801 caacaaacga ctccttcgat ttttgtgcca gataaacgac aggagctgta aataaaaaag
    22861 aagcaagaaa cttgagacag aatcatgtcc tagtttcttg tttccttttt attttttgat
    22921 aagaaataga tctacttcaa tggcagataa gaaagatcgg catcttctac caattgccag
    22981 tgtcgatcgg tgacagagta gattaaagta gcgatgattt cctcttctcg ataccgggtg
    23041 ccttttaatt tccaaatata atcttcaatc acatagattg ggatacgata tttaccatcg
    23101 atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct gcatcaaatt acgcatttct
    23161 ttcaataatt ccacactgat tttttcaaca ggtgtttctt ttttgaaaaa agattttttt
    23221 acattgatct tatctgacaa gatcgaactt aatttactag caaaaatcct ataaaattca
    23281 tctaaatagc gataataaat ccgattcagc tgatcattaa gatcgaagta tacaaaatta
    23341 ttctgtagtt tataaaaaaa cggcgaatga agatgtgttt tcatatgacc aaaatataat
    23401 aattctgaaa tttctaaagg tgtcaattct ttcagcatcg ttaaatcagt aaagtcgatc
    23461 cacttcaatt catcgaatcc acgcctgtta cgagaagagg aaaagaaacg ctgaatattt
    23521 tctgtaccac ggattacttt cagccctgta tgggattcat attctccagc agaagcagat
    23581 ggatcaagca agagcaagtt ttgaggaaaa tgcacgatac tgtttgcaaa gtcagtagca
    23641 gtcagccctt ttgttaaaac agcattactt gtggtatcaa tatgtacata aactgactca
    23701 gtcatcatgt tcctcctctt atttcctctt aatctctttt attttacaac atttgcatta
    23761 aaaaagcttg tttcttttat tttaaaattc cattacaaaa tcacgttttc attgaaaaca
    23821 ctctcatttt caattaattt ttgtgatttt tgtatttttt atttaaagga tgttttatga
    23881 attgttttct tatggaatct acaaagtcga atacgagact catgaccttt ttatgacgta
    23941 ctggtatact tgaagaaaaa ggagagcgcg atggagataa aaaaagttta cttttcacag
    24001 aaaatgtcgc tgtattcgcc tatccagcaa gcaaaacaac cccttccatt ttttcaaagt
    24061 catcaagaaa gtagagacgc tttttggcgt tctgcaaaaa agcaatgtgc ttttaaagca
    24121 gtatcgaaaa aatataccgt ctattttact attgctaaaa gtagaggaga tgttattttt
    24181 aataatctgt tgattgaagg tgaaactttt gaatggaaaa aattttttcg gtatatggaa
    24241 gcttgcgctc gtttttttat taaagaaaac tgttgttttt tatttcaatc tcctctatca
    24301 gaagaatggt tgcgtatttt tcataaaaac ggctatcaag gaaccacaca attaaataaa
    24361 aagctggtct atcatacagc acttgtttta ggtggaggag gagcacacgg tgcttatcag
    24421 attggtgtgt ggcaggcgct gaaggaacat gatatcaact ttgagattat cacaggtacc
    24481 tcggtaggtg cgttgaacgg cgcattgatt ttacaaggag atatgaaaaa agctgtgaat
    24541 ttatggaaaa agctgtcgac aagacaagtg cttgcgttac cagaaatggc tgcagttgaa
    24601 gaccttcgtg aacgcttcta tcgagaaaga cgccaaatga cgaaaacagc actaattgaa
    24661 ggcggggttt cttctgctcc attggaaaag atggtcaaag acaatttgga tttgtcctta
    24721 ctcaaacata atcccaaaat acgtttatat acggtgtcta ctaaactgcc agaactagcg
    24781 gaagtagtga ctgatatcca acagacaaaa acagaagaaa taccagattg gatactcgcc
    24841 tctgcttcat tttatcccgc tatggcttat cgaaagatcg gaaaaaataa atatgcagac
    24901 ggtgggtatc gcaacaaaat acctattgat attgcgatta acaaaggagc aacagaggca
    24961 tttgtcgtag atgtgcaagg gccaggccca gcgaaaaaaa ttcgtatgcc agacattttt
    25021 atccattgga aatgtcaaac attgtggaca ctaggcagct ttctgctttt tgatagccaa
    25081 aggaaccaat taaatttaca attaggctat ctggaaatga aaaaacgatt aggctattac
    25141 tatgggaact ggtatacatt tcattctgtt aaacctgcaa gaacatactg gcgaggattt
    25201 ttgtcttact taacaaaaga aattcagtta gaattatctt ttttcaaaag cataaaattt
    25261 tggcaaaaac tgcgtaattt atataagaac agggtcgtac cagagacatg cggattggcg
    25321 atgctagaac ttttagcaaa aaaacatttt ttactgccca atgaaatcta tcaagtagac
    25381 atgatgattc aaatgatctg ccaacaagat ataaaaagta tgccggatga tttattggca
    25441 caaattggtc aattaagtac tgaagaatgg cgaaggtatc gaaaatacca gcaaaaaata
    25501 caaaatgagc gcacgaaaac tgtttatttt gattatctgc tacaaggaaa acgcaaggat
    25561 cgtttgcagc atgaattgct taagcaacca gttgacacat tactgatcct atatttatat
    25621 tatctcaagg aggaacaacc atggcacaag aattttcata tgaaatcgta gaagaaatcg
    25681 ctatattgtc agaaaacaac aaggggtggc gtaaagaact gaacctagtc agctggaatg
    25741 gtcgcccgcc aaaattcgat cttcgagatt gggcgccaga ccatgaaaaa atggggaaag
    25801 gcctgacatt gacgaacgaa gagtttgaac aactgcaaaa agcaatagaa aatatgtaat
    25861 gaggaaggac cctgtcgaat gaaaagtatg actggttttg gcaaagccat ccgcgaaaca
    25921 aaagcgtatc agttagatat tgaaataaaa agtgtcaacc agcgttttct agatgttcaa
    25981 atacgaagtc ctaaaatcct gaactttgtt gaaaacgatt ttcgtcagct tattaaacag
    26041 cagttaagcc gcggtcgaat cgaagtattt gtgaatctag cttatattgg caaaaacgaa
    26101 aaacagatcg tcatcaactg ggaattgatt gattcactta tcactcaatt gacagacgga
    26161 attcaacaac gatacggtag tcaaaatcaa gtgtcaatta gtcggatggc ggaacgcttc
    26221 gcgctggacg aaaacttcgt aacggtagaa gaaaaaccgg aagagagcat ggaagaattg
    26281 gcacagttag ctctagatac attgaaaagc gcacttgatt ctattgaggc gagtcgagaa
    26341 aaagaaggtc aagctttagc tttagtgttg aaaaagaata ctgaagaatt caaagaagta
    26401 ctaaaaaaac tcgatagttt tgtagaaatc tatgaaaagg aatttcaaat aaggtatcaa
    26461 aaaaagctgg aggaatttct tggagcgaaa gtcgatgagc aacggttgtt gacggagcta
    26521 gccatcttgt tggaacgcgg agatattcac gaggaactgg atcgtctggc gatccatgta
    26581 caaaaactag atgaacttat cgaagcacaa tgtcctgtag gtcgggaatt agatttcctg
    26641 atccaagaaa tgaatcggga aatcaacacg atcggttcca aatcaaatgc aatcgaaatt
    26701 aaaaatcaag tcgtccagtc taagacgatc ttagaaaaaa tcagagaaca agtacaaaat
    26761 attgaatgac gaaagaggta gagataatga aatattatga cgtaacgttc cacgaattaa
    26821 gtggaaaaac tgtggttaaa cgggatatcc cttctgaaaa aaacggcttt gacgtatgga
    26881 aagatgcctg tgcagactat aatgagaacg aactgtttat tttgatcaac gacggtgctt
    26941 acgtgacgat gaatcggaaa tttattgttc gaattgacac agaagaggta gaagatccga
    27001 cagaaaaagc acgttctcga aaagatgaga tcatgggtgt agtgaatact ttgtccaata
    27061 tgggctttta gcaaggatgc tgaacaaaga aatacaataa ataatcagaa ttggatttct
    27121 tcgcttagct tctttgctct aggaatacat ttaaaggttg tgacaaaatt cgtgtcacaa
    27181 tcttttttta ttttgaataa gtgatgttca aaaaccattt tctcggtatt aagccaaatt
    27241 tatgcaaaaa tgtgagaaac tattttcaca aaatttttct tcatactcga aactaatcgg
    27301 cttcttcacg acctcttata cacggcgttc aaaagcttgc atctgcgata ataaccccaa
    27361 ccaaacgaaa aacatgagaa gctgtttatg tttggctgtt cttattactt gatgcaggac
    27421 agctttttta cgacctcttg gtacagggta gcttttttac caatttctag ctataattaa
    27481 agataacctt gaaagttgca gaaaaaaagt gcaaaataaa cgataagtac aaaaagacta
    27541 gttgaaagga cgtcgtcatg tcagagcgtg gattattaat cgtgttgtct ggtccatccg
    27601 gtgtaggaaa aggcacagta cgaaaagcaa tttttgagag tgaggataat gattttcagt
    27661 attcgatctc aatgactacc cgtaaaatgc gtgaagggga ggtcgaaggc gttgattatt
    27721 tcttccgaag caaagaagag tttgaatcaa tgatcgaagc tggagagatg ctggaatacg
    27781 cagaatatgt cgggaattat tatggaactc ctttgtcata tgtcaacaag acattggatg
    27841 aaggaaaaga tgttttcttg gagattgaag tccaaggagc aaaacaagtc aaagaaaaag
    27901 tgcccgatgg tgtattcatc tttttgactc cgccagattt agcggaatta aaatcacgga
    27961 tcgtaggccg aggaacagat gcagatgaag taatcgaaga acgaatgaaa gttgcaaaag
    28021 aagaaatcga aatgatggct ctttacgact atgctgttgt aaatgatgaa gtgccgctag
    28081 cagttgaacg aatcaaaaac attattgcca gtgaacattt tcgagtagat cgcgtaatcg
    28141 gtaaatatat taaaatgtta aaggagatgt agtcttatga tgttaaaacc ttcaattgac
    28201 tcactattag atcgtgtaaa ttcaaaatat tctctagtga ttttagcaag taaacgcgcg
    28261 catgaattgg atgcaagtgc acagcctact ctagattctt ttgattcagt aaaaagcgtc
    28321 ggtcaagctt tagaagaaat cgatgcagga aatgtagtca atgatccaca tcctgaatta
    28381 aaacgtgaac gtttgaagat ggaagaagag gaacgtcaag cacaaaaaga gcgtgaacaa
    28441 cacgaattgg aatcacgtat ccgtgaagag caaaaaatgt aataaagaaa aatagagcga
    28501 acatctaaaa agaaaagcaa acccagctgt ttccttttca aacattcgcc acttattgtc
    28561 tggtagaaaa ggctgtgtct gaactttttg acacagttct tttttttctt atctgctgtt
    28621 aattttttgt taggatttgg cagaagataa gacaaatcca tcgatctcta gtaaaatagg
    28681 caaggaactt tttgtcgctt gataagaaaa gacaaaaaaa ggaggaaacg aaccatgaca
    28741 ttaacagctg aagtcattgt ggacgtacct actatgcaga ccgatcagcc attcacttat
    28801 ttggttccct ctgaaatgga aacagctttg caagtcggga tgcgagtaga agtccctttt
    28861 ggtaatggca atcgtcatgt ccaaggattt gttatggcta tccaaaaatc agaagaatca
    28921 gctaatccat ctttgaaggc agttattcgt ttgctagacc ttgctccggt ggtcaatgaa
    28981 gaattacttt cattagctga ttatatgaaa aaaattactt acgcattcaa aatcacttgt
    29041 ctacaaacga tgcttccgag tgtgatgaaa gcagaatatg ataaattgat ctatcctttg
    29101 gcagacacac cagaagttca ggtattgttt gatcagcgag aagtgatttc ctggaaggaa
    29161 gcagaagaag aaggcagcct ttctcaattg attcgctggc gccaagagca attagtagat
    29221 atcaaatatg aagtccatac acgaaataaa gtaaaaacga ttcgtttggt ccgttctttg
    29281 ttaactgaaa agcaaatcga agaagaatgg gcaaaattac ggcaaaatgc caagaaacaa
    29341 aaagaacttc ttttgtgttt atctgaactg agccaagaag aaccaattgc ttatttcaaa
    29401 aataaaggaa tcagtactgc tgtcttgaat caaggaaaag aaaagggctg gctggagttt
    29461 atagaatcag aacgttatcg cgacccttat aaagaccgag tatttgacca aacgacggca
    29521 ttggaactga atgcagaaca aaaaaatgct gtcgaacaaa tcatcacagc aggtcagcag
    29581 caaaaagatc aggtcttttt gctggaggga atcactggca gcgggaaaac agaagtatat
    29641 cttcaggcaa tcgctgatgt tttgtcagaa aataaaacgg ctattatgtt agtgcctgaa
    29701 atcgcattga ctccgcaaat ggtcgagcga ttcaaaggac gcttcggaga atcagttgcg
    29761 gtcttacata gcggactttc ccaaggtgaa aaatatgatg aatggcgcaa gattgagcgg
    29821 gaagaagcgc aagtagtagt aggcgcacga tctgctattt ttgccccgct gaaaaatatc
    29881 gggctgatca ttatcgatga agaacatgaa tcaagttaca aacaagatga gacaccgcgt
    29941 tatcatgcga gagatctagc aatttggcgc agtaaatatc atcattgccc aatagttcta
    30001 ggaagcgcga cgccctcttt agaatcaaga gcacgagctc aaaaaggcgt ttatcagtta
    30061 ttgcagttaa accaccgagc caaagcagct gcacagcttc cagctattga gatcgtagat
    30121 atgcgtgaag agtttcagaa tcatcggacc tctacttttt ctgctaattt gcaggaaaaa
    30181 atacaaaatc gattagacaa aaaagaacaa actgtcctac tgctcaatcg tcgaggatat
    30241 tcgtcttttg tgatgtgtag ggactgtgga tttgttcttc catgtcctaa ttgcgatatc
    30301 tctcttacac tgcatatgga tacacgttca atgcgctgcc attattgcgg acatgaagaa
    30361 cctattccca atcgctgtcc taattgtggc ggcaataaaa tccgctacta tggaacaggt
    30421 actcaaaaag tagaagagga actccgagaa ctttatccgc aagctaggat tttgcgtatg
    30481 gatgtagata ccactcgaag aaaaggggca cacgaacaga ttttacaaaa attcggtgca
    30541 aaagaagcag atatattatt agggacacaa atgattgcta aaggtttgga ctttccagaa
    30601 gtgacattag ttggggtgtt gaatgctgat acctccctga atttaccgga ttttcgttct
    30661 agtgagcata cattccaatt gcttacccaa gtttcaggac gagcagggcg agcggaaaaa
    30721 gcaggcgaag tcgtgatcca aacattcaat cctcaacatt acgccataga attggctaaa
    30781 aaacaaaatt atgagcagtt ttatcagcag gaaatgcatg tacggcatcg tggaggctat
    30841 cctccttatt actttactgt gaaaataacc gccagccatc ctgaagaaca agtagcggca
    30901 aagaaaatct ttcagattgc caatcagcta aaagaagtgt taagccctca aagcttgcta
    30961 ttagggccaa cgcccagcat gattttgaga gtgaaaaacc gatattatta tcaactaatc
    31021 atcaaatata aacatgaacc gaatcttcct catgttttgg atgaaatttt gaatggaagc
    31081 cagaaagaac agcgacaagg tctttttgtg gcaattgaca acgaaccatt aaactttatt
    31141 taagaaaggg ccaaattatg cgctatccta tattgatcca ccctaatgat aaattaaaaa
    31201 gaactgctca gccaatcgat gtaatcacgg acgagacaat cgctttatta gacaatttat
    31261 atgagacaat gattgcgaat gacggaatag ggatcgctgc tcctcaagta ggacaaaaca
    31321 agagaattgc tgtgatcgaa gtagatgaag gcgagaaatt tgaactgatt aacccagaaa
    31381 tcatcgaagc aaaaggtgaa agccttgacg tagaaggttg tctcagtatt ccgcatgtct
    31441 atggaacggt caagcgagca gatgaagtga ccgtgcgtta ttatgatcga gacggagaag
    31501 agatagaagt gacggctttt ggctatttag cccgtgcctt ccagcatgaa atcgatcact
    31561 tagacggtat cttgttcatt gagaaaatga tacagcagat cccggaagaa gaacttgaag
    31621 aatatatgga ggaacatctt gatgactaag ctagtattta tgggcacacc tgctttttct
    31681 gtgccgattc ttgaagggct gatagaatca ggttacgaga tccaggcagt ggtaacacag
    31741 cccgaccgtc cagttgggcg gaaacgagta attacgccga caccggtaaa agaagcggct
    31801 ctgaagcacg gtatccgtgt gctgcagcca gagaaaatct ccggttcgcc agaaatggaa
    31861 gaaatcattg aattggtgcc tgatgtgatc gtcactgcgg catttggcca attcttgcct
    31921 gaaaagttat tgcaagtacc aaaattaggg gcaatcaatg tccatgcttc tttacttcct
    31981 aaatatcggg gaggtgcgcc tgttcattat gccatcatga atggggagaa agaaactggt
    32041 gtaacgatca tggaaatgat caaaaaaatg gatgcaggcg gaatctatgc gcaagaaagc
    32101 ataccaatca ctaaacaaga tgatgtaggg acgatgtttg aaaagcttag ccttttagga
    32161 agaaagctat tgctagaaac attgcccaat attttggatg gacaaaaacc agtacctcaa
    32221 gacgagtcag aagcaacttt ttcgccaaac atcacacggg aacaagaagc aatcgattgg
    32281 cataaaactg cagaagaaat tgacaaccaa gtacgcggaa tgcgtccttg gccaattgca
    32341 ttcacgacct atgagggcat acgctggaaa ttattatcgg ttgaagtatt ggcagaaact
    32401 acagacaaag cacctggtac gatcatccgg aaagacaaaa aaaatctatg gattgcttgc
    32461 ggtaaacaga ccgtcttagc catcaatgaa ttacagccag ctggtaaagg aaaacaggcc
    32521 gtacatgatt tccttaacgg aagcggtcag caagttcaga ttggtcagca ggtgaaataa
    32581 tgaaaaaaat ccctaaaaaa atgaaaaaat cagtacgata tatggcatta ctgacattag
    32641 agcgagtgaa caagggtggc gcctactcga atcttttgtt gaatgagatg atcaaacaaa
    32701 gcgagctaaa tcaaaaagat gtgggtttat ttacagaatt gatctatggt acgattagcc
    32761 atcagcgact attggaattt tacgttgcac cattgatcgc taaagcaaaa aaagtggatg
    32821 actgggtcaa gacgcttctt tatctttctg tataccaatt agaattttta gataaagtcc
    32881 ctgcacatgc tattttaaac gaggcggtcg aaatcgcaaa agtaaaagga aatcctggga
    32941 cagggaaatt tgtaaatggt gtcttgagga attatcagcg tcaaggagca ccagatctgc
    33001 aaggcattgc tgatcctatc gaaagacttt ctgtcaaaat cagcctgcct ctatggatga
    33061 ctaaacgact ggtggatcaa atcggatacg aagaaactga aaagctcggt ttatcgctat
    33121 atcagcctag ccatgctagt gcccgagtag atacacgccg actttccaga gatcaggcca
    33181 tcactgtttt gcaagaagaa gagatcacag ctgaaaaaag ccaagtcagt ccatacggta
    33241 tagtagcgaa aaaagggcat ttagcttcta gctcattgtt ccacgatggc gtcatgacga
    33301 tccaagatga aagttcgatg cttgtagcgc cgtctatgca aatcgagcct gaacatcatg
    33361 tcttagatgc ctgtgcagca cccggaggta agacgacgca tattgcttct tttttagatc
    33421 cgtcatgcgg tggagaagtg gtcgctttag atgtacacga acacaaagtc aaactcattg
    33481 aagataatgc agcacgtcta ggtgtctctt ctgctgtcta cgctcaaaag atggatgcta
    33541 gagaagtcag taaacagttc gaagcagagc agtttgatcg gattttagta gacgcgcctt
    33601 gttcaggtct tgggctgctt cggcgaaaac cagatattcg ttataaaaaa cagccggatg
    33661 aattagcgaa tttaccaaaa atccaattgg aaattttaga aagctgcgca ccaactttaa
    33721 agtcttccgg tattctgact tatagcactt gtacgatctt aaaagaagaa aaccaagacg
    33781 ttgtttctgc tttcttggaa agacaccctg attttgagag gattgatgtt ttgaccaatc
    33841 ctatgctaca atcatcaatc gaagagaaaa tgttgacttt gtatccgcat cagttttaca
    33901 cagatggatt tttcatttgt tgtttgcgca aaaagtaatg aggtgaaaaa atgcagattg
    33961 aatatcaatc agatgtagga agacgacgta acacaaatca agactatgcc agtgtattta
    34021 ccagccgttc aggtctcaag cttgctgtat tagcagacgg gatgggtggt catcgtgccg
    34081 gagacattgc cagtcagatg gcggtaacga atctaggcga agcttgggaa aaacaagaag
    34141 ctgttgacga tgaaaaaatc gcccaatggt tcattcaatc gattcaagaa gaaaatatgc
    34201 tgatttatca gcgtggacaa gaacagccgg aatataacgg tatgggaaca acaatcgtag
    34261 cggccgctct gtcagatgac tggttcacga ttgccaatgt tggggatagc agagcttatt
    34321 tgattcgtga agataaaatc atgcaattga cggaggatca ttctttagtc aatgaactag
    34381 tcaaatcagg agaaatcagc gaagaaatgg cagtgaacca tccaaggaaa aatattttaa
    34441 cacgttctgt cggcatgcct ggaacagtcg aagtagatgt ttctactcat ctttggcaac
    34501 taaaagatcg actgctgtta tgttcagatg gcttgacgaa catgctgtct gaagaagaaa
    34561 taggaacgat catcaatcaa gaaggcccat tgtctcataa agtaactgag ctgattgatc
    34621 gtgccaatga agcaggcgga gcggataata tcaccgttct tcttatcgaa tataaggagg
    34681 acagtgtatg atcgagttgg gcaaaaaatt aaatggccgt tatcaaatca ctggaaatat
    34741 cggtagcggt ggtatggcaa atgtattttt ggcgcacgat ttaatcttgg atcgtgatgt
    34801 agcagttaaa gtcttacgat ttgactttca aaatgaccag actgcgattc gacgatttca
    34861 gagagaagct ttagcagcaa cagaactcgt tcatccaaac attgttagtg tttacgatgt
    34921 tggtgaagaa gacaacatgc agtatctggt gatggaatac gtcaaaggaa tggacttgaa
    34981 acgatatatc caaacacatt atcctgttcc ttatgaaaca gcagtaaata ttatgcagca
    35041 gatcctttct gctatttctt tagctcataa ccatcaaatc atccaccgag atttgaaacc
    35101 ccaaaacgta ctgatcgata atgaaggtgt tgtaaaaatc acggatttcg ggattgctat
    35161 cgctttatct gaaacatcga tcacgcagac gaatacgatg ctaggatctg ttcattatct
    35221 gtcccctgag caggccagag gaagtatggc aacgaagcag tcagatattt atgctttagg
    35281 gatcatcctt tatgagatgt tgacgggtag cgtaccgttt gatggtgaat ctgctgtaac
    35341 aattgcttta aaacattttc aagatgatct tccatctata aaagcacttg atccaaatgt
    35401 gccacaagca ctggaaaacg tgattttacg ggcaacggct aaagaacctg cagaccgtta
    35461 taagtcagcc gaggaaatgt ctgatgatct ttctacagta ttatcaccag ctcgtgcaaa
    35521 cgaagaaaaa tggcagcctc atgtgatgga taatgaaaca aaagttatca caccccttac
    35581 ggaagatgca ccaatgcctg attcattcaa atctatgcct ctgccaaaag acaagccaga
    35641 aaacgaaacc ttaccagagg aggaagaagt tctgcctgat cctaaaaaga aaggacagaa
    35701 aaaatggtgg ctgatcctat tagctattct tgctgtttta ggaatcggaa cagctgtatt
    35761 ctttgcttct ggcgggcgag gagaagttgt tgttcctgat gtttctgatc tttctgaagc
    35821 ttctgctaga gaaacactcg caaaagcgaa attagaagta gcaacaaaaa cagaagaaat
    35881 tgcagatgac aaaatcgaag aaggtaatgt agtgaagaca gacccggcag cgggaacgac
    35941 agtcaagcaa aaacgcgaag tcactctgta tattagtagc ggcacgaaaa aaatcaaatt
    36001 agatgattac agtggtaaaa gttatgaaga tgccgaagca agtttgaaag aattagggtt
    36061 ttctgctgac ttgattcaaa aagaagagga attcagttca gaggtagaaa gcggaatgat
    36121 catttcgcaa tctccatcag aagatacaga agtcgatcct aaaacagaca cgattacttt
    36181 cacagtcagc aaaggaccag aaacagtgac tttgagtagt catgtaggag aaaactacga
    36241 aactgttcgt aattggcttg ttacacaagg attcaatatc aataatatta gtgctactta
    36301 tgactatagc gacactgtac catcaggttc gatcatcagt caggatccag cctcgggtga
    36361 agttgtagca gaagaaactt acatcaattt tgtcgtaagc caaggaacag agcctaaatt
    36421 gaatgatatc tccggctata caaaaagcga agcacaaagt tatttagcta gtgtcggtgc
    36481 agaatatatc gggcatgaga cttccgaata ttcaaacacg gtggataaag ataaagtgat
    36541 tcgtacagta ccaggggcag gaacgacaat taccaaagga atggttgtga acgtaattta
    36601 ctctaaagga ccagatccag cgtcttctag ttctgaaaaa gactcttcag acagttctag
    36661 cagctcaagt agctccagta gcacaagcaa ctcttcaaaa gaagaatctt ccagctccac
    36721 aagttcatca gctagcacct cagaaagttc aaaataagtg ctgaataaaa tatatcagaa
    36781 gggaatgcga cattgatttg ttgcattctc ttttttgtgt ttctatctct tttgatcaat
    36841 tttcaagcca tatactttga gaagtttgca tggaataaca cctactttcc tcttcgtatg
    36901 gtaaaataaa caaaaacgga ggtggcactt atcaaaggac aaatcagaaa agcaatcagc
    36961 ggtttttact atgtatacgc aaacggagag acctatcaaa caagaggaag aggaaatttc
    37021 cggaatcgga aaatcacgcc gcttgtcgga gatcaggtga tctttgagag tgaaaatcaa
    37081 acagacggct atcttctgga aatcttgccg agaaaaaatc aattagttcg cccaccagtc
    37141 acaaatgttg atcttggaat cattgtcaca agtatcgtag agcctgcatt ttcttataat
    37201 ctacttgacc gctttctagt taccttggaa tacgagtcga tcgaaccgat tatttttttg
    37261 acaaagaccg acttgtcaga cgacccagaa aaaatcgaag agatcaagca aacctatact
    37321 aaaataggct atccggtgat tgtacccaat catacgggtg atacgacaga actgatccgt
    37381 tacttcccag aacggctatc ggtctttatg ggtcagtctg gagcaggaaa atcgacgcta
    37441 ctgaatcaaa tatcaccgga actgaattta gcaacaggag agatttctga tgcacttgga
    37501 agaggacggc atacaacacg tcatgtggaa ctgattccta tctatggcgg gttagtagca
    37561 gatacaccag gatttagctc gattgacttt ttgacaattg aagcaggaga actgccgaga
    37621 caatttcctg aatttgtaga agcttctgtc cattgcaaat ttagagagtg catgcatgct
    37681 aaagaacctg gctgcgaagt aaaacataga gtagaaacag gtggtatcgc acagacacgt
    37741 tacgacaatt atttgcaatt cttacaagaa atcgaaaaaa gaaaaccaat gtatgcaaaa
    37801 aaagaaggta aacgaaaata ggaggaaaca agaatgaaga tagcaccatc cattttgagt
    37861 gcagattttg ccaatttaca aagagacatt gagttagtgg aaaaaggagg cgcagattac
    37921 atccacgtag atgtaatgga tggccaattt gtcccaaata tcacattagg gccaaatatt
    37981 gttcaggcaa tccgtcctgt tactaaatta ccgttagacg tccatctgat gatcgttgat
    38041 ccagaaaact acattccagc atttgcaaaa gctggagcag atatcattac tgtccacgtc
    38101 gaagcaactc ctcatattca tcgggcactt caaatgacaa aaagcttagg agttaaagct
    38161 ggagtagtta tcaatccagg aacaccagtc tccatgatca aacatgtttt gcctttggct
    38221 gatcaagtac tagtcatgac tgtcaatcct gggttcggcg gacaaagctt tattgaagaa
    38281 acagtagaaa aaattacgga actcgctgat ctacgtagag agaacaattg gacttatgca
    38341 atcgaagtag atggcggaat cgttccagaa acagcaaaaa tctgtaaaga tgcaggggcg
    38401 gatgtcttcg ttgcaggatc atatatctat ggagccgaaa atccagtcgg acaaatcgaa
    38461 cgattaaaag aggcattaga ctaaatgaaa attttattag ctgctggagg taatccgcaa
    38521 aactggccgc agtctctgct gtccaaatac gatgcctatg tcgggataga ccgtggtgcg
    38581 ctgtacctgc ttcaagcagg ctacccagta gatctggcag tgggtgattt tgattcattg
    38641 tcgaacgaag agcagaagga agtatttgca aaagccaaag agtatacccg ttcccctgca
    38701 gaaaaagacg atacagatac acagttaggt atcttaaaaa catttgaacg ttacccgcaa
    38761 gcggaagtca ctttgattgg agctacgggc ggaagattgg atcatctttt agccaatctc
    38821 tggttaggta tggaacctcg cttcaaaccc tttgcccatc agcttattat cagagattgt
    38881 caaaattatt tgtcttatta tttgcctgga gagcatatag taagacagat accagatatg
    38941 aagtatttag cttattgctg cttgactccc gtctcgaatc ttacattaaa aggcagcaag
    39001 tatgtattgg aaaataaaga agtagaatat ccgacatctt atgcaagtaa cgaatttctt
    39061 tcagataccg cagaattttc tttttcagat ggtatgattg cagttattca aagcaaagat
    39121 tgatccataa acaaataaaa aaatttgtct ttcaggctaa aaatgacaga atctattatt
    39181 tgctaaaaaa aagcttaaga ggagcaactt accttttgtt tcacggcatt tagtgataaa
    39241 attgaacaca agagtctaat taattcgagg tgtctttgtg aagaagttca atcctaataa
    39301 aaatattatt attaccttgg ttattgttat catcgtggtg acgatactaa gtgtgaccgc
    39361 tgcgcagcga gcgaatgaag gaaagcccaa tttcttccaa tccatcgtta atgacagtgt
    39421 tgcaatggtt gataaaacaa tctctgcccc agtacggtgg gtacgaaatg gagtagattc
    39481 tgtccataat cttttttcaa cttatgccga aaatgagcgg ctaaaagaaa aaatcgatag
    39541 ctatgatgac gtttcacagc aaaacaagaa ttacgagaga gaaatcgaag cgttgaaaaa
    39601 agaacttgag ttgaatcaga cgctgactag ttacgaaaaa gtaacggcaa atgtcattac
    39661 tcgttcgcct gacagttggc aggacatgct gatcgtagac aagggttcta aagacggtat
    39721 cgaagcaaat atggcagtca tgtcgcaaaa aggattgatt ggccgagtga tcgaggtcaa
    39781 tacggcttcg tctaaaatcg aattactgtc atcctctaat gaaagctcca atcattttcc
    39841 agtacgggta tcttcggcta atggcgaagc gtttggtttg cttaaaaact atgatgaaaa
    39901 gctccatgcc ttagtggtga cccaattaac tggtgatacg gatatcaaag aaggggatgt
    39961 tgtccagaca tccggtcttg gagggaattc tccagctaac ttgccgatcg gtacggttat
    40021 taaaacgaaa ccagatagtt atgggctgga tcgggaagtt tatgtgaaac cttatgcaga
    40081 aatgtatgac gtgtcagttg tgacgattgt ccaacggcta gcggaggatt agtccatgtt
    40141 gaagaaagaa acaatgaagt actatctgcc aatcgttttg ttctttttga tgttgataga
    40201 tggtcattta acaagaatgc taggggagtg gtcgaaaggc acctatatgt caaatgccca
    40261 ctttctgata ttggcattat tatgttgcag tatggcgttt gaaaaacgtt atttactgat
    40321 taccacgatt gttctcgggg ctatctatga cgcttactat attggcgtta tcggtatcta
    40381 tgcagtagct ctccctttaa ttgtatggtt gatgtatgta atgaaagacg ttatccatgt
    40441 caacatcttt actgaatttt tcagtatgat catctttgtc acgggttatg aattgtttac
    40501 gatggtggtc cagttgattt ttaaattagc agtagtaaat aacacgtatt ttattacaag
    40561 gtttttagga cctacactgc tgttgaacat gattatattt gtattattca tttttccctt
    40621 taagaaatta ttcagtgatg aataatttct ttttttattt ttaggtcgaa agcgaaaaag
    40681 aatcgttgtt atcttaagaa atgctagact taacgcggct ttttcatgat cattttctga
    40741 aacgaacagt tttgtcattt ttaaaagttg ttttgaaaca tttttgtaac ataactatta
    40801 tatcgttgac acactgctat ggtacaattt ttatatcgtt aagaaagact taaatatatt
    40861 tagatattta ttaaattaag ccagtcggag gaatgaagag tgaaaaagag tttaatatca
    40921 gcagtaatgg tatgttcaat gaccttgact gccgtagcat caccgatcgc tgccgcggcg
    40981 gacgattttg attctcagat acaacagcaa gatcaaaaga ttgcagactt gaaaaatcaa
    41041 caagcagatg cacaatcaca aatcgatgcc ttagaaagcc aagtttctga aatcaacaca
    41101 caagcacaag acctattagc aaaacaagat acattgcgtc aagaatctgc acaattagta
    41161 aaagacattg cagatttaca agaacgtatt gaaaaacgtg aagatacaat ccaaaaacaa
    41221 gcacgcgaag cacaagtaag caacacaagc tcaaactaca tcgatgctgt attgaatgct
    41281 gattcattag cagatgctat tggacgcgtt caagcaatga caacgatggt aaaagcaaac
    41341 aacgacttga tggaacaaca aaaacaagat aaaaaagctg tagaagacaa aaaagctgaa
    41401 aacgatgcta aactaaaaga attagctgaa aaccaagctg ctttggaatc tcaaaaaggc
    41461 gatcttttat ctaaacaagc tgatttgaac gtactgaaaa cttcattagc tgctgaacaa
    41521 gcaactgctg aagacaagaa agctgacttg aaccgtcaaa aagcagaagc tgaagcagag
    41581 caagctcgta ttcgtgaaca acaacgtcta gcagaacaag ctcgtcaaca agctgcacaa
    41641 gaaaaagctg aaaaagaagc acgcgaacaa gcagaggctg aagcacaagc tactcaagct
    41701 tcatctactg cacaaagctc agcaacagaa gaatcatcag ctacacaatc ttcaatgact
    41761 gaagaatcat catcagctac acaatcttca gcaacagaag aatcaacaac acctgaatct
    41821 tcaacagaag aatcaacagc gcctgaatct tcagcaacag aagaatcaac aacagtgcct
    41881 gaatcttcag caacagaaga atcaaaaaca gtgcctgaat cttcaacaac agaagaatca
    41941 acaacacctg cgcctacaac accatcaaca gatcaaagtg ttgatacagg aaacggcaca
    42001 ggaagtagta ctccggctcc aacgccaaca ccaacacctg aacaaccaaa acctgtaaca
    42061 cctgctccag ctccttctgg aagtgtgaat ggtgcagcaa tcgtagcaga agcttacaaa
    42121 tatattggta ctccatatgt ttggggcggt aaagatccaa gtggctttga ctgctcaggg
    42181 ttcacacgct atgtttacat gcaagtaact ggtcgtgaca tcggtggttg gacagttcct
    42241 caagaatcag ctggaacaaa aatttcagtt tctcaagcaa aagctggaga cttgttattc
    42301 tgggggtcac aaggcggaac ttaccacgta gcgattgctt taggcggagg acaatatatc
    42361 catgctcctc aaccaggcga aagcgtaaaa gttggttcag ttcaatggtt tgcccctgac
    42421 tttgctgtca gcatgtaaga atgattaatc aaaaagagtt agcccattct ggactaactc
    42481 tttttt
//
LOCUS       DACEUF010000015        41223 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.016, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000015 DACEUF010000000
VERSION     DACEUF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.016"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            125..1168
                     /locus_tag="IXG20_000784"
     CDS             125..1168
                     /locus_tag="IXG20_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ1608998.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1230..1694)
                     /locus_tag="IXG20_000785"
     CDS             complement(1230..1694)
                     /locus_tag="IXG20_000785"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ1608999.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            1940..2887
                     /locus_tag="IXG20_000786"
     CDS             1940..2887
                     /locus_tag="IXG20_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1609000.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(3098..5170)
                     /locus_tag="IXG20_000787"
     CDS             complement(3098..5170)
                     /locus_tag="IXG20_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1609001.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(5103..5480)
                     /locus_tag="IXG20_000788"
     CDS             complement(5103..5480)
                     /locus_tag="IXG20_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ1609002.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(5609..6169)
                     /locus_tag="IXG20_000789"
     CDS             complement(5609..6169)
                     /locus_tag="IXG20_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1609003.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            6319..7611
                     /locus_tag="IXG20_000790"
     CDS             6319..7611
                     /locus_tag="IXG20_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1609004.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(7671..8789)
                     /locus_tag="IXG20_000791"
     CDS             complement(7671..8789)
                     /locus_tag="IXG20_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ1609005.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8803..10224)
                     /gene="arcD"
                     /locus_tag="IXG20_000792"
     CDS             complement(8803..10224)
                     /gene="arcD"
                     /locus_tag="IXG20_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ1609006.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(10470..11591)
                     /gene="mnmA"
                     /locus_tag="IXG20_000793"
     CDS             complement(10470..11591)
                     /gene="mnmA"
                     /locus_tag="IXG20_000793"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ1609007.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNDKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            11995..12594
                     /locus_tag="IXG20_000794"
     CDS             11995..12594
                     /locus_tag="IXG20_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ1609008.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(12732..14018)
                     /gene="efmA"
                     /locus_tag="IXG20_000795"
     CDS             complement(12732..14018)
                     /gene="efmA"
                     /locus_tag="IXG20_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ1609009.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(14289..14633)
                     /locus_tag="IXG20_000796"
     CDS             complement(14289..14633)
                     /locus_tag="IXG20_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ1609010.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(14770..15906)
                     /locus_tag="IXG20_000797"
     CDS             complement(14770..15906)
                     /locus_tag="IXG20_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1609011.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(16095..17069)
                     /locus_tag="IXG20_000798"
     CDS             complement(16095..17069)
                     /locus_tag="IXG20_000798"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1609012.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(17149..18024)
                     /locus_tag="IXG20_000799"
     CDS             complement(17149..18024)
                     /locus_tag="IXG20_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ1609013.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            18154..20061
                     /locus_tag="IXG20_000800"
     CDS             18154..20061
                     /locus_tag="IXG20_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ1609014.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(20602..23880)
                     /locus_tag="IXG20_000801"
     CDS             complement(20602..23880)
                     /locus_tag="IXG20_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ1609015.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(24151..24696)
                     /locus_tag="IXG20_000802"
     CDS             complement(24151..24696)
                     /locus_tag="IXG20_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1609016.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(24708..25400)
                     /locus_tag="IXG20_000803"
     CDS             complement(24708..25400)
                     /locus_tag="IXG20_000803"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ1609017.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(25419..25703)
                     /locus_tag="IXG20_000804"
     CDS             complement(25419..25703)
                     /locus_tag="IXG20_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609018.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(25716..26279)
                     /locus_tag="IXG20_000805"
     CDS             complement(25716..26279)
                     /locus_tag="IXG20_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1609019.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            26507..27169
                     /locus_tag="IXG20_000806"
     CDS             26507..27169
                     /locus_tag="IXG20_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ1609020.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            27293..28489
                     /locus_tag="IXG20_000807"
     CDS             27293..28489
                     /locus_tag="IXG20_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ1609021.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(28590..29966)
                     /locus_tag="IXG20_000808"
     CDS             complement(28590..29966)
                     /locus_tag="IXG20_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1609022.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(30003..30869)
                     /locus_tag="IXG20_000809"
     CDS             complement(30003..30869)
                     /locus_tag="IXG20_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ1609023.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(30958..31983)
                     /locus_tag="IXG20_000810"
     CDS             complement(30958..31983)
                     /locus_tag="IXG20_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1609024.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(32001..32675)
                     /locus_tag="IXG20_000811"
     CDS             complement(32001..32675)
                     /locus_tag="IXG20_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1609025.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(32859..33752)
                     /locus_tag="IXG20_000812"
     CDS             complement(32859..33752)
                     /locus_tag="IXG20_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1609026.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(33826..35523)
                     /locus_tag="IXG20_000813"
     CDS             complement(33826..35523)
                     /locus_tag="IXG20_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1609027.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(35516..36829)
                     /locus_tag="IXG20_000814"
     CDS             complement(35516..36829)
                     /locus_tag="IXG20_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1609028.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(36829..40347)
                     /locus_tag="IXG20_000815"
     CDS             complement(36829..40347)
                     /locus_tag="IXG20_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1609029.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(40640..41098)
                     /locus_tag="IXG20_000816"
     CDS             complement(40640..41098)
                     /locus_tag="IXG20_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1609030.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT        11620 a         8908 c         7368 g        13327 t
ORIGIN      
        1 tattcgaaac aagaggtcgt acaagacttt tgtcacgacc tcttgttctt ttgtcaaaat
       61 actggtttag tgctactatg ttcgagtatg cttttagtat ttattgacga aaggaaccga
      121 tcaaatgaaa tcattgtcac atccaataaa atctgtaaag gattcagcgg tccgcgaaat
      181 cttacccggt ttactgcttt cttttgccat cgctgccttc agcaggcttc ttgctattta
      241 tctgccacag ttaggtggag cgactattgc tattttattg ggtattttat taggaaatac
      301 tttttttaaa cagcctgttc tagcaatcgg cacaaaattt gctgaaagca aactattaga
      361 attctctgtt tttttactag gtgcaactgt cacagctcaa acgattgctc aaattggtgt
      421 aaaaggaatt gtttttgtgt tactgcaaat gtcagtcact atcatcggtg cttacttgat
      481 tggtaaaaaa ctgcaattca gcgatacttc ttctttgatg atggctggag gaaacgctgt
      541 ttgtggttct tctgctattg cttcaatcgc cccttccatc catgcgaaag aggaagaaaa
      601 aggacagatc atcacactag tcaatctact gggtactgtg atgatgttga ctctcccttt
      661 tcttggatta cagttgtttg gtggaaatct cattgaaaaa agtgcacttt tgggtggtac
      721 actccaatct gttggtcaag tagtcgctgg ggcaagctta cttgacaatt ctgttgtcga
      781 attcgccatg ctatttaaga ttctacgtat tatgatgctc gttgttgttg tctatctgtt
      841 tgaaaaattc atgcacaaga aacaaccagc tctgtttgaa acgccaactg atacgcagaa
      901 aaaacgaaaa ttaccgcttc cttggtatgt attagggttt gtgatctttt gtttggtcaa
      961 cagcttgttt tctttacctg tagttattga ccaatcggca cattttatca gcagctggtt
     1021 tgagatcact gctttagcgg caattggtct gcgtttagac tttgcaaaat tcatgaaaga
     1081 aggtacacgc ttcttatctt atggattagc tgtcgggctt ctccaaacag tgatggcttt
     1141 actatttatt tggctgatca aagtttagaa tgaacatgaa aaaacagttg tgattctcgc
     1201 atggaacaat gtccgaatca caactgtttt taattttgca gatatccttc taacaacaac
     1261 aattggcgtt tgatttcaac cccatctatt gcgtttccca tatatcctgt cagttttcca
     1321 ttttgcccga tcactcgatg acaaggaata acgattggca atggattggt tcgatttgcc
     1381 tgcccaatcg ctcgcacagc tttcggacta tcaacagcaa cggctacttc tttatacgtt
     1441 cggatttctc cataaggaat ggtcgacaaa gctttccaga cattttgctg aaaaggcgta
     1501 cctctcttta tagataaagg aacatcaaat tcttttcttt ttcccgcaaa atattcttca
     1561 aactgttctt gtgtgcgcgt taaaattcgc tgattcgctg attcgctttc tgaaattggc
     1621 tgaaaagata cagcagtcaa tccggattca tctgcatcta accataaaaa gccgataggt
     1681 gtgtgtattt tcattttgtt tgctcctagt caatagactg gaacttcctc ctttttctct
     1741 atccaaaact acttgccaac taataaaaat tgttcttaaa agtttgataa accgaggaaa
     1801 catggtaaac taaatacgtt agtttacaaa aagaatacct gttcttttac gtaaaatcaa
     1861 attttaaagg tgttatttaa ggtttattaa attttgaatg taaaccatat ataaacatac
     1921 gattgaaagg atagaaacga tgaaagtatc aattgttgtc ccatgtttta atgaagaaaa
     1981 aactgttcct cttttttttg atgaggttga aaaattcaaa ggtgcccatg agttcgaata
     2041 tttgtttatt gacgacggat caaaagacgg tacgttagca gcaatcaaga agctagcctc
     2101 agcttatcct tatgtccgct ttgtctcatt ttccagaaat tttgggaaag aagcggctct
     2161 gtacgctgga ttgcaagctt ctacaggaga tttagtgacc gtgatggatg ttgatctgca
     2221 agatccgccc gaactgcttc ctgagatgat caaccggatc actacttctg atatcgattg
     2281 tgtcggcacc cggcgttcga ctcgtgaagg cgagcctgcg atacgcagtt tctttgcgaa
     2341 aaaattctac cagttgatca ataaaatcag cgatacagaa atggtcgatg gcgctcgtga
     2401 tttccgcttg atgactcgtc aaatggtgga tgccgtccta gaattaacag aatacaaccg
     2461 attttcaaaa ggattattta gctgggtcgg atttaaaaca gaatatattt cttatgaaaa
     2521 ccgcgaacgt gtggctggcg aaacatcttg gtccttctgg aaattattca actactcgat
     2581 tgatggtatc gtgaacttct cggatgcacc gttaacgatt gcttcattta tcggcgcttt
     2641 gtcttgtgtc ggttctggta ttgcaatttt gttcattatt ttgcgggcat tgttgtttgg
     2701 tgatcctaca tcaggatggc cttccatggt ctcgatcttc ctgttcattg gtggaatcca
     2761 attgttatgt cttggaatca tcgggaaata tattgggaaa atcttcttag aaacgaaaca
     2821 gcgtcctgtt tatatcataa gagaaacaga aaaagtgaag ccaaatgaat tgaagcctca
     2881 ccaataatat acgtcaaatg aagtaaacat ttttttaaat gaaaaaggac tgtgacaaaa
     2941 gtcttgtcac agtccttttt tccgaataaa tggtgttcca gaagtagctt cttcggaaat
     3001 aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
     3061 agctgaccac ttctatcaca acctctttca tatcttatta cttcttcatt gctgctttta
     3121 gtgctgctgc taatgggctt tcttctggtt cttctgcttg tgcatatttt ttcagcaagc
     3181 gtcgttcttc atgtttggtc attttcttct tctgctcttt tttatcaagc aatttttctg
     3241 tcgttccatc gtatttacaa cggaaataag caccgttttt accttcgatg atttccattt
     3301 tccggtgaca ttgcgggcat cgatgattag agacttttgg atcttttctt ctgcggtagt
     3361 tgcaatctga attggtgcag acatagattt tgccgtcacg tgtgtttttc tcgcgcagat
     3421 tggccccaca ttctgggcat ttcttttggg taatcgagaa atcttgatat tcttgtttgc
     3481 ttgatttgat ctcattcacc aagcgttttg tttctttttc gattccattc aagaaggttc
     3541 ctgcttgttt ctttcctgca gcaatagctt caaggtcttt ttcccagctt tctgtcaaat
     3601 ctggtgttac taaagaggga tttaccaata ctaataattg cttaccttta ggagatactt
     3661 gcagtccttg aggggtacgt tccatcagtt cagatttgat tagtttctcg ataatctctg
     3721 cacgcgtagc tggggttccc agactgtatt tttccatttg accaagcaag cttgcttctg
     3781 acaatggttt aggcggtgca gatagttctt ttttgatggc gaaatcggga ttgattctca
     3841 ttcctttttc ccatttgact gtttccgtct cagtttcagt ttcgcctttc catcctgctt
     3901 ggacgacacg attttgccga aaaatgaatc gttctttacc aaattcaacc gtaacttttg
     3961 tttggctgac tttatggggt tgagcaaaca gtcctaaaaa acgagagaca atcatttgat
     4021 agatttttcg ctcttcatta cttaattttt cataacgtgc tctgttttca gttggcaaaa
     4081 gtgcatggtg atcggtcact ttactgtctt ggaatacttt tgtctgttgg acgatggctc
     4141 cgtttttcaa atactgcttg acctcaggag caaagtcgct gactgcttgc aatcgttctt
     4201 tcatcgtact tttcatgtca tttgtcagat atttgctgtc tgtacgcgga taagtaacga
     4261 tcttatgggt ctcatacaaa ctttgtacaa gacttaatgt tttctttgca gaatagccat
     4321 agcgttgatt ggcttctctt tggatctctg ttaaatcata aggcaatggc gcagattccg
     4381 tttttaattt ttcttggact tctgtgacag ttcctttgct tgtactgatc aattgaacaa
     4441 gtttttgtgc ttcttcttgt gtttttaaag caaattgatt tttttgaagc attttggctt
     4501 gttcttttcc agcttgcaac gtgatcgtga aatacgtttg tggacggaac tgctcgattt
     4561 tcttttcttg gtctctcact aatgctagtg tcggtgtctg cacacggcct gctgataaat
     4621 tatccgcgta cttcacagtt aaggctctgg tcacattcaa tcctaccagc cagtccgctt
     4681 ttgcccgggc aagagcagat tcatacaatg catcatattc tttcgcaggt cttagttgct
     4741 tgaatccagt tttgattgct ttatctgttt gagaagagat ccataaccgc ttcactggtt
     4801 tattgaaatg cacatactct aagatccagc gagcaacaag ttccccttct cttccggcat
     4861 ctgtcgcaat gactgcttct gagacatctt ttcgctgagc cagctgcttg attgctttta
     4921 attgatgtcc tgttttagga agtggtttga ttcctagttt tttagggatc attggcaacg
     4981 tgtccatttg ccagctctgc cattccttat tcagatcttc cggcattttc aatcctaata
     5041 aatgaccaag ggcccaagta acgatcacct tgggtccttc ataataattt ttgtgctttt
     5101 gattagcacc aagcaccttg cttaggtctt tcgcaacgct tggtttttcc gctagaatta
     5161 gttgtttcat aaaattcctt tctgctttct actgtgatgg gctgatgatt tttcaacaac
     5221 gcaagtccct tgtattgctg taaatcatcg tcacactctt cacaccagcg ctcatctccg
     5281 tcattccaaa aggcgctgag aaagttactt tttgcattga tatattcata attttgatgg
     5341 atttcgttcc attgtttttc gtacgcttgt cgtgcttctt cgaaagactc gaatgtttct
     5401 tcttcttcaa tatcttcttc ccagtcttca aaaaaccacc aaggctcatt gtccccgaac
     5461 atggtaatta cttgatacat actcgtgctc cttctttcag cttttctaga ctcatcttag
     5521 tcacttttca agaaagttgc aagaattttc tcttataaaa gggtcattta atgaacatct
     5581 taaaattgtt acaaatgaat ctgattgttt aaggcaaagt agcgataaag ttattgatca
     5641 tccaaaaata gctgtcttct ggatcgattg ttggttcttt tgtaaagtat cctaaaaaac
     5701 gtaaggaaag ataaccgtgg atcatactac gaagttccct gcttcgatga actttttctt
     5761 ctttcgttaa ttgaaaagcc tcaaatatct gcaaaatgat ctgattcgtt tcatggattc
     5821 catctaataa aacagcttca ttggtatgcg gtacactgat cagtaactca tacactgctt
     5881 gatttgaaaa agcaaaggat cgataagttt ctgcatagat ctgcagtgct tcctgaccac
     5941 tttttcccac aagagcccgt cgcagctggt cattaagttc ctgcagaagt ttcgctgtta
     6001 gcgctgtttt gacatcacga atatttttga aatggttata cagcgaggga gccttgatat
     6061 ccaagcgttt ggctaaatgc tgaaaggtta tttgctgaac atccatctct tctgctagtt
     6121 ctcgaaagca gtcgatgatt ttctcttgag acagatttct ttttttcatt tccatgtttc
     6181 ctctcataaa ataaatcctt cactctttca gtttaccacg aaaaagaaag agaaaaaaga
     6241 gactttcatt gataaactac atattgtagt ttataataaa gagctgtaga tgaaaatttt
     6301 cagaaagaag gagtctaatt ggctgcaaga ataaaggaaa aattatttag gaaaagagaa
     6361 acttgggaac aaaacttgtt tgtcttatgg tttggaacat ttatggctgg tatcggattt
     6421 agtttagtca tgccttttat gtcactttac atagatacgt taggtaatta tagtacgtca
     6481 caattgaact tttggagcgg attgactttt tcatctactt tcttggtgac tacgcttatc
     6541 tctccttggt ggggaaaatt agccgaccaa aaaggaagaa aattgatgct gttgcgtgct
     6601 tcattaggaa tggcaatcgt catttctttg atgggttgcg tgactagtgt gtatcaatta
     6661 gtcgcattgc gtctgcttca agggattttt tctggttata tcagtaatgc cactgccctt
     6721 gtcgcaactg ggacgccaaa agaaaaaagt ggtcaagttc ttggaacctt agcaactggt
     6781 tctgttacag gaacattact tggaccgctt cttggcggga tctctgcttc agcgtttggc
     6841 tatcgcccta cctttttcat tacagggatc atcttatttt tcgtcttctt gttgagtctg
     6901 tttttcgtcc atgaaaaatt tgtcccagtg aaaaaagaaa acatggtttc tgccaaacag
     6961 attttcaaag acttgaaata tccacacgtc gtcatcggta tgttcgtcac aacgatgatc
     7021 atccaagcat ccaacaattc cattagtcca atcatcagtc tgtacattcg ccaattgctg
     7081 catggacatg ggaatgttac gttgatcagc ggaatcattg cttcgatacc tgggattgcc
     7141 actttgatag ccgctccaag atttggtcgc ttaggtgaca agattgggag tgaaagaata
     7201 cttgcgattg gtttgggttt tgcgattctg gtttatatcc caatggcatt cgtcacgaat
     7261 gtttggcaat tagctgcctt acgttttttg attggtatct ctgatgcttg tctgctccct
     7321 gctgtccaag cattgatcac aaaatattcg cctcatcaag ctgctggacg gatcttcagt
     7381 tataaccaat ctttccaagc aactggtaac gtagtagggc ctatgatcgg ctccagtgtt
     7441 tccagtgcat ttggttatcg tggcgtcttt ctttcgactt ctcttcttgt tttggtcaac
     7501 ttcttgcttg tacgacacaa tacacaagag atccatcagc aagaaactgc agaagaaaaa
     7561 gcttcaggag tctccatagc ttcagggcac tctccttctc atagacaata agcgtaaatg
     7621 aaaaacactg cttgaacagt tgtcacagac tgtccttgca gtgtttttgt ttaggcctgt
     7681 tttttcaaga actcagataa gaggaataat cccttttcca aggtttcttt tttacaacag
     7741 taacctagtc gggcatgctt cggtaaatca aaggcacttc ctggtactaa tagtactcct
     7801 gtttcttcta ataaattgat acaaaatgtc tggtcatctt cttcgatggc taatttaatg
     7861 aaagaagtag agacgtaatt gggagcgaca agttcaactt tcggttcatt ggctacccat
     7921 tgttttaaaa tcacaaggtt ctctgtaatg atctttttgt ttcgcgccaa tattttttct
     7981 ttattcttca atgcatgtac agcaagatca tctgacagga ctccgccgca aatcatcgtg
     8041 tagtctcgat actttcgaaa aacttctgcc aactcaggtc cagtagctgt ccagccaata
     8101 cgaatacctg gaatggagta ggttttggat agggaattgg ttgctatacc tttttcatag
     8161 acatctacga tcgacaagaa ttcaccttta tccgttaaag gcgcatatac ttcatctatc
     8221 aatacatatg catctactgt acgagcaatt tctgcgattt tttctaaagt tttccgatct
     8281 agcaatgtgc ctgtaggatt gtttgcacta ttcaaacaga tgatttttgt ttcaggtttg
     8341 actaaggctt ctagctgttc taaatcaaac tgccaatctt cttcttcctt caaatgaaca
     8401 aagtcaacag cggcacctaa agatttgggg atgtcatata attgctgata agtaggaagc
     8461 atcgaaacaa catggtctcc tgcttccact aacgcgtata gagctaaaag attagcacca
     8521 gtcgctccat tcgtttgtaa gatgttttcc ggatcgacgg tttgataaag agaagcgact
     8581 tcttgtttga aaacatctga tccttcgatc cagccatagt ccaatggttc gtttttgcgt
     8641 ttttcgaaaa atttacttac gctagtccca tctaagccaa cgagttcgtc taatgtcaat
     8701 gcttcgatcg agctttgtgc aatatcatat accgctttct tttcccattt atttagccat
     8761 tcttctacac cgaaatttgc gattttcatg tttctttccc ccttatatct tgatccagcc
     8821 tacagcaagc aacccgattg aaagcagtgc ggccacacaa atcagcccaa tcattacttt
     8881 ttcttttccc gtaatttgat gtttggtttc acgacaagcg atccagtaga agcaaaaacc
     8941 aggaagatag gcaatactac ataaaaggac ataagaaaat cctgctaaaa tgatgcccat
     9001 caattcaaaa actacagcaa tcccaccgat cactacttgt tttttgattc cttcttttaa
     9061 agaaaactgg acttgataga tcccgacaag tagataacaa atcaaaattg ccgctgtaca
     9121 taaagaatag gcaaattgat aggcataatc agtaactaag aaagtcaata agaatagatt
     9181 agtcaagatt gcagtaacaa ctaacgaata agttggggca ttttttttat tcgtttttcc
     9241 ccaacctttc ggcaacagac cgtctcgagc catcaacatc gttgtttctg ctgggagcat
     9301 cgtccaagac aaccaagaac caatgatcga aataatcaag ccgatattga tcagcgctgc
     9361 tccccaattt cccacgattc cttttaaaat atttgccata gcaggttgtg aaatagttgc
     9421 taactcttct tgagataaga cgccgtatgg caagatcgaa gcaaggatat agataaacaa
     9481 taaacaaagc agaccaatga tcgatgcttg ctgggcttct gaacgtttct tcgcgcggtt
     9541 ggctaaaaca cttgcgcctt cgatccctac aaagacccac atcataacca tcaaacaacc
     9601 tttaatctgc aaccagatcg ttccttgctg ccctgttcca ttcactagat tatcagatac
     9661 gttaccccaa aaatcagcag tgaatatatt cactttaaat gcgataaata caaatattaa
     9721 aaataggaat aatggcacta atttacagat cgtcacgatt gtattgatga cgctcgctcc
     9781 ttcaactcca ttgtttacta ataaagtcag accccaaata aaaatacttg caaataaaat
     9841 agaaggaaca ttttgtccac ctttgaatat cgggaaaaat tctcccatgg cgctcatcag
     9901 catcgttgca aaagcaacat ttcccagcca cgcagacaac cagtaacccc aaccactgat
     9961 gaagccacct aatttaccaa atgaagcttc cgcgtaaccg aaaataccac catctagatc
    10021 tggacgcttt tctcctaaat gattcagcga taataccaaa gctaaaatac caatcccaac
    10081 gattatccat gaaaggatcg ctggccctgg agctgcagaa ctagccagat cacttgtgat
    10141 cccaaacaca ccgcttccga ttgacgaact gacaaccaga gcaaccagtc cccatttgct
    10201 aatacccgtc gatttctttt ccatttccat caccctcact tataaaattg aacaaatcac
    10261 ttgcaaaaga ttcccttcta ttttgcaact gctcttgctt aacaaaaagc aactttattc
    10321 atattattgg atattatgaa ctttttacac aagtatttca agtataaaat gaataaaaat
    10381 taattttatt gcaaaaaaac agctatgcta tccaaaagaa aaggtgtttc ttttgttagc
    10441 catagctgtt ttgattcatt taatatcgct taaacgtatt gtaaaacttt tgtttcttga
    10501 tatgcgtggt cgatcaaacc accacccaga cattccattc catcataaaa tacaactgct
    10561 tgtccaggag tgattgcacg tacaggttcg tcgaatacga cttctgcacg attatcatcc
    10621 aataagcgaa ctgtcacagg aacgtcttgc tgacgataac ggaatttcgc tgtacacttg
    10681 aattcttttg gcattggttc attcgtcgta aagtggattt cacttgcatc caagcttgtg
    10741 gcatacaatg caggatgatg gaacccttgc cccacatata atgtgtttgt cgctagatct
    10801 tttcctacaa caaaccaggg ttcttgcgaa tctcctccgc caccgatccc tagtccttga
    10861 cgttgaccaa tcgtataata catcaagcca gcatgttgtc ctttgacttc accatcaaga
    10921 gtgaccatat ttcctttttt tgctggcaga tagttgctca aaaattgttt gaagttcttt
    10981 tcaccaataa agcagattcc cgttgaatct tttttcttag cagtcgcaag gcctgcgcgt
    11041 tcagcaattg cccgtacttc tgatttttcc atgccgccta gagggaacat cgtttttgaa
    11101 agctgttctt gtgataattg gctaaggaag taagtttgat ctttatcatt gtctactcca
    11161 cgaagcatgt ggaccacgcc gttttcatcg cgagtgactt gcgcataatg tccagtagct
    11221 acataatcgg cgccaagatc cattgcataa tccaagaatg ctttgaattt gatttctttg
    11281 ttgcacatca cgtctgggtt aggtgtacgt cctgcgcggt attcggctag gaaatattca
    11341 aatacacgat cccaatattc tttttcaaaa ttcacagagt aataaggaat cccgatttga
    11401 gcagctactt tcgctacatc cttataatct tccgtggccg tgcatacacc gttttcgtcc
    11461 gtgtcatccc aatttttcat gaatattccg atcacgtcgt atccttgttc ctttaacaaa
    11521 agtgctgtta cagatgagtc gacaccgccg ctcatcccta cgacaacgcg tgttttgctg
    11581 ttgtctgtca tgatatcacc atcatttcaa ttagattctg aaacatctac gagttgaagg
    11641 tcgcaacttt tcagtatttt acattatacg tcatatttct gaaaatttca tcccttaatt
    11701 atcaagtcgt aaaaatactg ttctactgga cctattcagt acacaaacat gatagtcaac
    11761 caaaaaatca ctcatctttt gagcattcga tataaaaaat tggcatactc ataaaaaggg
    11821 ccagcctttc ggcaataagg aataatttta taaattgtga aaaactattt ttcaaaattg
    11881 cttcttatcg cttaaagctg cacgtctcat ttacaacctt ttctttttag atgttatgag
    11941 ttgctcagat aagtgatttt gtttactcta agaaaaaagg gaggcaaaag atacatgaat
    12001 tactttatca gtgatatgca ctttttccat gaacgcttat taggaaaaaa cgactttgct
    12061 ccgcggctct ttgcaaatgt ggatgaaatg aaccaacagt tgatcactag ctggaatgca
    12121 gtaataaaag aaaccgacca tgtttatcat ctaggcgacc ttgctatgca tccgcaatat
    12181 gaaaaagcaa atgcagaaat tttggaactg gtcactcaac tgcaaggaac aatccacttg
    12241 atcaaaggga accatgacag tcgcgctttt ttccgttatt tagaaaaaaa tgatcctagg
    12301 cttactgacg gaagtcccaa attttatttt tatgatgtag gtgccatcgt taaattcgac
    12361 catcaacaat attacttgac tcattatccg atgttgctcg ggcctaacga cactatccgc
    12421 aatcttcatg gacatatcca tcattacagc gtacctatcg agaacgatgt caatgtaggc
    12481 gtagacgcac cggaaagaga actcttaaag gaaaaactac catttggcac tccgttatct
    12541 gagcaacaaa tcaaagaaat ctatgaagca aagaggaagt acgataggtc gtgaaaaaac
    12601 tgccctatat caagtattaa acaaataaag ataattactc atatttttca tttgagtagg
    12661 cttattgcca caggaacatt aggatcagaa atggagttgt aacaagactt ctgtcacaac
    12721 tccattccat actattcgtc tttttgctca tggtgttctt ttaccagttt tttctgtaat
    12781 tctttatcat aatttcgggc agctgacgtt aaaaataaaa cgattccgca taataaaatc
    12841 ccgattccgg aaaacaagaa cattttttct acccctaaac gatcagctaa tggtcctgca
    12901 aataataatc caattggacc tgccaggttc atcaaagagt tgaagttccc taagacacga
    12961 cctaaaacat tcgattcata actctgctgg atcattgcca tcagcagtgt attgaaatat
    13021 ggcgttgcac aacctgctcc tgcattcaag ataagaaagt acaagaaccc ttgactatcg
    13081 tttggaacca atccgcttgc accgatcgtc aggcccataa tgatgtaggc tataataata
    13141 ggtttcattc ggtctttcca tgttcctata aaactgatca gtgcaccgcc taagagcatt
    13201 ccgactgcgt agactacttc cactgctccc gcctgtccaa cggaaccacc aaagtaatct
    13261 agcgtcatca atggatacag actgattgct ggcatgaaca acagcatcac aaatgcacca
    13321 ttgatcgtta tgtaccataa ccctttatta tccatcaatt gctgcagccc aaattttgca
    13381 tctttaaaaa tagtaagcgg aacttccaat atttcaggag aaacttttgg gatcttcaca
    13441 aaaatcaata agccgacacc aaaaactgcc cccaatacat ctaataaaat aaggtaattg
    13501 acaggaacga ctgaaaacaa agccgcacct agagctggcg caatgatgaa attcgctgat
    13561 tggaccattc ctaactgtcc attcgtcctt gttatgtgag aagatggaac gattgtcggc
    13621 ataatcgact ggatcgttgg catttggaat gtttgtgcga ccgaacgcat aaacaaagac
    13681 acaaaaacta gccaaatcgg gaaggattct gaaatcgttc cgactactgc taaaataagc
    13741 gcgaaaatcg ctacaataat atccgtgaca atcaaaagag cttttttatc ccagcgatcc
    13801 actagcggtc ctacaaatgg gcttagtaaa accatgggaa tcattcctag taaagtagca
    13861 aaactcaaaa ttgtcgccga accggtttct cttgtcaaat accagatgat cgcatattgg
    13921 acgaccatac tagtgattcc tgacaaaaac tgtcccgata aaaagaacaa ataatttctc
    13981 ttccaattcg ttaaaacgac cgactgttca ttttccataa acatccctct tttctttttc
    14041 attttgtata gcgtttgcac aacaatcatt atacgggatt cttttttcaa aaaatacctt
    14101 tttccctaaa taacaatcgt ttcaatttaa atttctttac ttagaaacaa taaatcagca
    14161 aaatattaaa aataacaaca tgtgttaaat ttaaaaataa aaaagacaac taatttgcgt
    14221 cgtccttaca aagttagtca tctaacaata ggccgtttca aaactagttg tctttttata
    14281 tccacagatc attcgacttt ttcgaataac ccacggctaa tcatcccatc catcagaaaa
    14341 taaaatttat cttggctcat tttctgtttt ggatctttga aaatattgac tgctttcaat
    14401 ccatttgctg tagtgatcga cattttcaat gtttgaatat cttttgatac agtgattttt
    14461 aatttgaatc cttctttgct ttgcggcgtg gcatctaatg gtcggatcaa ttgataattg
    14521 cctccgtttg tttctgtaaa tccattatcg cgcaatgtat atctttttgc atcaggatgc
    14581 aaacgatagc taattggtgc gcccagtacc gttgctgttg tttcaaatgc catgattttc
    14641 acctcgataa aattagtttc tctttattat aaaagtttat cctgattttt actaggtttt
    14701 tcagttaagt tccctaaaaa atgaaggcgt tctctcagac atctgttcat ataagaatga
    14761 cttcactagt tattttttga cagcacgaac taattgatca aggaaatact ggatgtcgtc
    14821 ttcggtattt cccaaaccaa agctgatacg gatggattct tttagtatcg gtgtatcttc
    14881 tccatacatt gcttctaata cgtgtgaagg ctcgatattt cctgctgtac aagctgaacc
    14941 agtagaaata gcgaccccat taagatcaag cttcatcagt agcaaatcat ttgcttcgcc
    15001 aattaagcga agattcaaca catgagggag tttattcgtt aaatcaccat ttacttgata
    15061 agggatttcg tttgcttcta atgtatcaag aatcaattgc tggaatcctt tatatttgtt
    15121 gttacgtgct tcacgttctt caggtgacag cagttcaact gcttttgcca taccccagat
    15181 ccctgctaaa ttttctgtac ctgcacgccg cttttcttcc tgctccccgc ctaaaagcaa
    15241 tgcagggata tttactccgt ctcgtttata taagaaaccg acgccttttg gcccattgat
    15301 tttatgagca gagatactta ataaatcgat tcctaattct tctggatgga tcgtttggtt
    15361 tccgtacgct tgtaccgcat ctgtatggaa ataagctgga tgatccgcta aaagttcacc
    15421 gatttctttg attggcatca aatttccgat ttcattgttc ccgtacatca cagagaccaa
    15481 gatcgtatct tctcgtaacg cttttttgac ctcttcgatc gacagattcc ctttttcatc
    15541 tacaggcaga tatgtaacgt caaacccctt tgtctctaaa tatttcatgg tattaagaac
    15601 agcaggatgt tcgatagctg tagtgattag atgtctgcct tctttttgac gcgagaacgc
    15661 tgtttctaag atagctgtat tatccccttc tgttccccca ctgttaaaga tgatctcgtg
    15721 aggttttgct tgcagactgt ctgcaatgat ttgtcttgct gtttccaatt tttcatgcgc
    15781 gtggcgacca aatccatgta tgcttgaagg atttccaaac gtgtcttgca tgattgccgt
    15841 catcgcttca attacttgtg gatgcatggg ggtagttgcc gcatgatcca gataaatagt
    15901 tttcacttca ttcatccctt ccaaattatt gctaggcaca ttctagcata aaatagcgaa
    15961 caaaccaaag aagagctcat ttttgtctag aggacgtttc atgggcatag aaaaaaggag
    16021 ctgtgacaaa agtcttgtcg cagctccttg aagctgctca cttctgtcac aacttctttc
    16081 cctctttttt ttgtttattc gaattctttt tcttccagac ggaataacgg actcatcggt
    16141 gtattttcat ggatgcgttt gaccgcttca cccattaatt ctgaacatgt aacgattgtt
    16201 aagttttctg gatgacgatt ttcttctgtg aaaacagaat cagtgataca aatatctttg
    16261 attggtgcat tgtctaaaat acttttcgca tgatcggaca ataatccatg tgacgcacag
    16321 gcatatactt cttttgcgcc gttttctttt aatacatttg ctgctcgtgc aagcgttgct
    16381 cctgtgttca agatatcatc taccatgata catgtctttc cttgaacatc accgatcaca
    16441 taaccaccct cagttgaagc atcatctgca tgatctacga tggctaatgt gctgttcaaa
    16501 tattctgaaa gactacgtgc acgctgaaca ccgctgtttt ttggcgaaac aactacgatg
    16561 tcatctcccg tcagacctaa ttgacggtaa taatgagcaa acaacggcat cgtaaataaa
    16621 ttatctacag gaatatcaaa gaatccttgt acttgaaccg tgtgcagatc taaagtcagt
    16681 actcgagttg cccctgcttc ttcgatcaag ttagcgatca atttcgcagt gatcggttca
    16741 tgaggtttcg ccgtgcggtc ttgcctagca tacccatagt aaggcaatac gacattgatc
    16801 gttttcgcac ttgctcgctt cattgcatcg atcatgatca gtagttccat atagtaatca
    16861 ttgacaggtg cattcgttga ttgaacgata tacacatgat cccctcgaat actttcttcg
    16921 atattgatgg aaatctcgcc atcactaaat tgcttcacga cgcttttacc taattctgtt
    16981 ccgaacactt tcgcgatttt ttcagctaat ggacggttgc cattcaaact aaaaatcttc
    17041 aacgtgtcat cctgatactt attactcatg tagtcctccc caaaacttct ctattcttcg
    17101 atagtttaac atatttttcc aagattttct gttaatttca gcaaattctt aaggaagtga
    17161 aactgttccg ttactccaac tggcaggaat gactttatga taatcgcccc agttgtcgtg
    17221 tactttgtaa taacgttctc ctgtttcagt ttcaaaataa gcgtacgctg tcacccaatg
    17281 attgccatat gtactgccaa gtactttcaa aataccaatc atcacaggtt tgccttcacg
    17341 tatacgtttt gtagctcgct gccatgaacc tagaagagtt gctcgtgcac gctcatgatt
    17401 gccattcttc tttaaaaaac tagaaatccc tgtactaacc tgaaaaggaa tcgtaggaag
    17461 tcctagtggt tggatctggc tacgcaatgc cttcgtcaaa accaacgaat cagccgtatt
    17521 tttcttgcga atctcatttg gaagcatcca ccccttacga tagtcttgat aataggcaag
    17581 gagaactgct gccgcgtaag taccacaaag atagcctgag ggggtagccc atttcttata
    17641 cctcaaaaaa cgttcttctg gcaatcccac ccagcaattt gcgagttcct ttcctgacct
    17701 tttttcttcg atcaaaacgg gaatatcttc ttttctgttg atccatctag ataactcgat
    17761 tggctcttca ttgatcagct tccatttttt acaagaaaga ccctcttgat aagtatattt
    17821 tgctggagaa tattctaccg gatgtcctga ttgatcgata atcacataac tttgatcttc
    17881 aaaaaccaga gctaaaaaaa gcaatttact ttttcttgac tgcagctgct gagtgctggc
    17941 taatttttta atttttttat ctactttgaa atataaaggt ttatccacta cttgattcag
    18001 ttgggatcgc ctcctttttt tcattataag tgaaggattg aaaaaaacaa atcagtttga
    18061 ttgttcgaat tttcaatttt ttatgttcta tttgttaatt ccttcatttt gtaagataat
    18121 gggcacacga aacaaaaaag gagcgatcga tgaatgaaca aagaacgatt gaaacaagat
    18181 ttctatgaag cagtcaatga agaatggcaa aaaacagcaa aaatccctgc agataaacca
    18241 gctacaggcg gtttccaaga tttagttgac gggatcgacc aacttttgat gggagaaaca
    18301 gacgatctgc ttgcccatcc agaaaaagtg tcttcagaaa aaatgaaaca tttccttgca
    18361 tattatcaac tggcaaacga ttatgaccga agaaatgagt tgcatgctca accgcttcaa
    18421 cctataatcg aaaaagttga atcattgcag tctttcgacg aattgaataa gcaatttcca
    18481 gaatggctgt tggatacatt accattgcca tttaatctgg atgtagatgc agatatgaag
    18541 aatgcgcaaa cgaacgcctt ctttgcttat ccgccttctt tattccttcc agataagacg
    18601 tattatgcat cagaccatcc caacggtgca caactaatag gcgttttctt tgatatggct
    18661 gttcagttat tagagcttgc cggcaaagaa aaaagtcagg cagaagcaat cacggaacaa
    18721 gcagtgcaat ttgataaatt gattgctcct catgtaaaaa gtgcagaaga aaacgcagat
    18781 tacagcaaaa tgtataatcc gcaatctttt gatacattta ttaaatacac agataaactg
    18841 gacttgaaaa aacttgtcac gggtttgatt gggacaactc cagataaagt gatcgtaact
    18901 gatccagtgt acttcgatca tttgaatgac atcctgacag aagctcattt tccgttattc
    18961 aaaagctgga tgatcattaa aacagtacgt tctcttagtg gttatctatc agaagaattc
    19021 cgccaagtca gcggaatcta ttctcgcgta ttgtctggaa cagacgaagc aatgcctcag
    19081 aaaaaagcag cgtattacct tgcctctgga caattcgatc aagtagttgg cgattactac
    19141 ggaaaaaaat attttggcga agctgctaaa aaagacgtag aacaaatggt gaaaaaaatg
    19201 atcgccgttt ataaaaaacg tctagaagaa aatgactggc ttggggatgc aacaaaagaa
    19261 aaagccgtca taaaattaaa taacttaggt atccaagtag gctaccccga taagatccca
    19321 gcattattcg accagttcaa aacagtacct gcagatcagg gtggtacttt gctttcaaat
    19381 actcttgttt tcagcaaact cacattaaaa gatcgtttca gcaaatggaa caaaccagtg
    19441 gatcgttcag aatgggaaat gagtgctgac acggtcaatg cttattatca tccatttcgg
    19501 aatgtgatcg tctttccggc agcgatcctt caagcaccat tttacagtct agaacaatca
    19561 agctctgcta attatggtgg aatcggagct gttatcgctc atgagatttc ccatgcattt
    19621 gataataacg gcgctttatt tgacgaatat ggcaacttga acaactggtg gacagaagaa
    19681 gatcttacac aatttcaaaa caaagcacaa gcaatgatcg aagaattcga tggtctgcct
    19741 tttgctgacg gaaaagtaaa cggaaaatta accgtttcgg aaaatattgc tgatgcaggc
    19801 ggtctaagct gtgcattgga agcagcaaaa acggaatcag atctttctat cgaggaattc
    19861 tttgtcaatt gggcaacgat ttggcgtaca aaagcaaaaa aagaatacca acaactttta
    19921 ctgcaaatcg atgtccatgc ccctgctaaa ctccgagcaa acgtgcaacc aaaaaacctt
    19981 gacgaattct atgaaacatt tggtatcacg gaacaagacg ctatgtatct agcgccagaa
    20041 aaaagagtgc gaatctggta agaggttgtg ccagaagtgg tcagcttcaa gaaataagaa
    20101 ggaatttggg aaaatcgctt ctcaaatttt tgtgacattt cggcttattt ttcgaagaag
    20161 ttacttctgg aataccatgt attagaggtt gtgaaaaagc tgcttagttt caagtagtaa
    20221 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttgactta ctaccgagaa
    20281 actggctttt gaacaccgtt tatcgagagg ttgtgaaatc agcgtcctga cctgagtatt
    20341 agagaaaaaa acggaaaaat agttcctcat attttgccgt ttgttgagct aataccgagg
    20401 gtcagctgat tgaacaccgt ttagtggaga gattgtgaaa aagctgtcct gcatcaagta
    20461 ataagaacag ccaatcaaaa gcagcaagga gaacctcatc tataaaaaga tagaaggaag
    20521 tgagacatct attggtctca tttccttcta tttttacttt cctcagtcaa ttgttttaca
    20581 tcataaaagg agaaattcca cttatttttt tcgctgtttc atgacataaa caactacacc
    20641 gattaacaaa attcctccag ctaatgctag caacgggtta ttaaattctc ccgtttttgg
    20701 gagtccttga gtgccatttt tttcactttc ttttgctgtc ctatccggat gagaaggttt
    20761 tccatttggt tttccatttg gtgattcatt tcctggattt tctctttctg gtttgtcatt
    20821 tcccgggggc acatcggcag tttctcgcac agtgatcgct ttttgctggg taacagattc
    20881 tgtatgagca gaccattttt ccccatcaaa tatttccgaa cgataagtga cttctagcgt
    20941 atattctcct tttgaaggtg ctgaaaaaat agcggtatga ttagaaaatt gagcacttga
    21001 ctgtgtttca acgatttgga tcttttctgg gaaataacgc atatcttcaa agacaggcgt
    21061 tccttgattt ggtgtgaaag ttgacacaaa aactgtaaaa ggcttcagtt caatgatatt
    21121 tccttctgca ttctccgcca caccatttgc ttcttgtata ataatcggat tattttgttc
    21181 atttggtttt gtctgatttg gttcttgtac gattgcatac tctggatatt ccgaagacgg
    21241 tgaggtttct ccgtcaaaaa tagcacctac agataatgga agaccatcca ctctttcctt
    21301 ttctgtttga cgattccccg gtttaatctg catcagtcca tgaacatgga tacttccatt
    21361 ttcatagcgc gtaacaggct gctgaccttc tttcgaacat gtatttagag actcgatcca
    21421 atcttttgat atggtatgtc caagagtatt ttctgattgt ttctctcgtt cattccaata
    21481 gtagttcaac gagtggtaga tttgatcttc gttttgattc aatgggcgga tctgctctct
    21541 catctcaaga ttctctgttc ggaaagataa gataccagaa gcatggtagt ctgtgtttcc
    21601 tgctgaagag gtataaagag ccacattata tgaaccgtta ttaaaacttg tactgctatt
    21661 gacgataata tctgttccgc tattggaatc gattcctttc gcaagatttt cgaaagacaa
    21721 actattgatc aattcatgag ggcccgttaa agaagaaccg cccattttga atccattacc
    21781 gttaccttct ccctcaattc ctggtaccca gccgtttcga taagcaactg agtttttgat
    21841 gattactttt ccgatcgaac ctgtttcatt tttagcaaaa aggtcccaac cgtcatctgc
    21901 attatgatac gcgatacaac cgtcaaatac atttccttct cctattgtca gtttggccgc
    21961 aaatccatcg gcatcttcaa atcctggatc ggcattttca aaagatgtac agttcttgac
    22021 taagttatgc gcgggccatg cttcgaaagg atcatttgag ctaccgctga tttggattcc
    22081 tgtatttcca ttccgataag catgaatctc ttcgatcaaa ttatgatttc cagaaatctg
    22141 taatcctttt tgcatagctg cactatttgt aatgtcaaac cctcgtaaac cccagtagtg
    22201 actgaacagt gtcatcccat ttccagttcc tttaaaatca agtacaggac gcttgttttc
    22261 tggatttttc tctgcaatca actgaatcgg gttttctctt gtcccgttaa cgccttttgg
    22321 aatcgtcaat cctctttcga aggaataagt tccaggcgcc atgacgattg tctgcccagc
    22381 atatgcgtat ctcaatgctt gtgtcagaga taatggattt tgtctgcttc cgtttcctgc
    22441 atctgaccct tctggtgtta cataaactgt cgaatattta ggtttacggt agtccactgt
    22501 atgaagaatc tcttttgttt cataactagt catctctacg tattcttctg gcggatagtt
    22561 tttatctggc gtaaaactga aagtaaattc gttcgttcct tccatcagtt ttgtctgata
    22621 atcaaattcc cgattcgcag tgatagccaa gttttttgcg ttcttaagca cctctcctgt
    22681 tgctttattt ttaattgtca ataatccgtc agcatttgct ctaaaggaca tagtatgatc
    22741 ttggtttcct gaatgattac tagaggtcac agtgtaaatc ggatcaatca agcgagtagg
    22801 tctttcttgc ccaggaggat ctgtcgcagg atctgagtaa gcaacttcga ttccttctac
    22861 cttgatcgtc atattccttg ctgcagcaaa tcctgcataa ataaccgatt catctgaagc
    22921 gtataatttt tcccagtcat acatgatgtc agaagccatt tcttctcctg tttcaggatc
    22981 gtaataatat gcctctaaac cagtattcgt ttttttcatc tctacgtcca gtttagtaat
    23041 cgagttcgtg gccggaaaca gattcccatg accgtttccg aatatattat aagaacctgt
    23101 ttctttttcc aagaaccctg ctgaagtctc taaaggtgtt gtttccactg agacactccc
    23161 aggagctggc gcagcttgcg gatcttcaga tgatattcct gtgattgccc gtgtccccaa
    23221 tccaagtcgc atcgtatatt tgctgccgct cgtgttgacc acttcttgtc tatccgagtc
    23281 ccagacatat tcgatccgac tgcctagtat ggcaaatgaa tttgaataaa atgcctgccc
    23341 aaacaaatga ttttttggta ctgcgtctct taccattaaa gcaaatcctt cttgggcatt
    23401 cgtataggtc caagattcta tttcgatcgt cgcccgcatc ctaaagttga tgtctttggg
    23461 gattgcttga taataatagg tcattccatc tgatccgctt gactggaatt ttccgccgtt
    23521 attcgtggaa gagagccgca cgccgtcttc taatggttca atggcatacc tactagcact
    23581 tgtgctttct ccgaaaacaa cccctttcca ctcttcattt gcttcttcag attgcttttt
    23641 tgtcttttca ttttctgatt ctttcatcat aggatcattt ccttcttccg tatccatatc
    23701 ttcactagtt cttggtattt cctccattgt tgacatccct tcaatgttca cgtgtgcatt
    23761 ttcttcgttc acagactgtt gttcgttagc agcaacttca acgacagaaa ttaaaaccgg
    23821 ggtactgagc ataaggagat tcatcattag cgcactgtat tttatacgtt tttttaacaa
    23881 gtgtattcct cccgtaacaa attagaaagc gtttacttaa agtattacct aatttatttt
    23941 tactgtcaat aaaaaacaaa attataataa gtgtatttta taaaaagaaa aaatataact
    24001 taagttgttg ataataaaga gatttattaa cgtaaaaaga ataagttttt ttaaaataac
    24061 gtataaaaaa gagcataaaa caaagtcgtt tgttttatgc tcttctttta ttattttgtg
    24121 ctttttattt tatcaaatca ctttgttttt ttataaaatt tctgcgccta gtgtggactc
    24181 aaaatgtctc aatgcccatt catgtccaac gggatcgaaa cttgccacgg catctttgaa
    24241 aatatggagt ttgtatccta gattatacgc atcgactgct gtatgcaaca cacagatatc
    24301 tgtacatacg ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac ggatgtctag
    24361 atctgttcca ctaaaagcgg aataatgccg tttgtctatc caataaacat tttcctcttg
    24421 ctcatagtct tggtataaac tttttaaaga gccgtacaat tcccgtccac ccgttcctat
    24481 cacattatgc ggaggaaata agcggttctc cggatgaaag gaatcttttg gatcatgcgc
    24541 atcaatggca aaaacgacaa aatccttttg atcaataaac ttctttgtat gagcagtcag
    24601 ctttttttca attacttgtc ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa
    24661 atcatatgtg tagtcaatcg agatcaatgc gttcatctta gtcctcctta tacgagatgt
    24721 ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca
    24781 aagctttgtg tcgccgagtg atctgctgtg tcgctcattg cacgtacgat caaaaatggg
    24841 atattgaact gacgagctgt ttgtccaaca gcggctcctt ccatttcgca agctaatgct
    24901 tcagggaaat tcgttaagat ttctttgatt ttatctggcg agtcgacaaa tgtatcacct
    24961 gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca
    25021 ctgcgcaaat atgtgcttgc ttcataatat agaggcattc ctggtaattg tcctgggttg
    25081 tagccaaatc ctgttgcatc tgcatcaaaa taagctactt tgtctgatat aacgatgtca
    25141 cctacttgta agccttcacc gattccgcca gctgaacctg tattgatcac catgttcacg
    25201 ccgtattgtt ggatcaacag acttgtggta atagatgcta agacttttcc tataccggaa
    25261 cgtacgacga tcacttcgtg gttacctaat gaacctgaaa taaataaagc gcctgcgcgt
    25321 tcccatgata atggttcgct taggttctct ctcaaaattt ttacttcttc ttccattgcg
    25381 ccgataatac cgattttcat ctttttaatt ccttctttct atataaatgc gattgctaaa
    25441 aaaaccacac ataacattag gataacaata ataagagatt tcatcaaaaa agagttccat
    25501 ttggtcgtct tttcccgttc gctgatcctt gttttcgtga ttggtttatt ctttttgctt
    25561 tctttacgat aaaagctggc aatctttttt tcttcttgct gataagctgt ttctgctttt
    25621 cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc
    25681 gtaataagcg gtcctttggc cataagccga tccctctact ttcctttggt caatagttcc
    25741 cagtattgaa tcgcataaat cgtctttgca tcacaaatca actgatcttt catcgcttgt
    25801 tgcgcctctt caagagttag atgatacaac tccagtactt cgtcttcatc ttgtgctaaa
    25861 ggattctcga ccttctctac cccttgtgca tgataaatat gaaggacttc atttgcaaaa
    25921 cctggtgaaa gatacatcga agtaagatgg ctcagcgact tcgcacgata gccagtttct
    25981 tcttctaatt ctctagctgc tgtcatttcg ggattttgtc cttctcctgg atcgattttt
    26041 ccagctggaa tctccaaaat cactttttct aaaggttttc gaaattgttt gacaagaagc
    26101 agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa taccagttct
    26161 ctcttcgaag ttcctccatc tggcaaacga acttcgtcta cagcaacatc aataattttt
    26221 ccgctataga tttctttgcg actgattgtt ttttcttcaa attgctcatt gttcagcata
    26281 tcttctcctg ctttctttgt aatcataact gctttttttt catttttcaa tttttctcaa
    26341 aaactcctta ttagtttacc atagataaac cttagatttt ctctaaatat agaaaatcct
    26401 tctttagtcc gatactcttt atagtagtcc gtatgctaaa ataagtatgt aatcgaataa
    26461 cgaaaagaat tcaatactca acgataaatg acatggaggg tttattatga taaaacgaaa
    26521 ttggccgtgg cttctagcat tggtcttgct atttctcttt gtccgaaaca atagtttttc
    26581 ttttgttcta ggacttttat tgataggtgt agttttagtt ttgtggggac tctttggttc
    26641 gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg actgacgaac ttgaaactca
    26701 ctatgaaaaa agcggtatga cagcgaatga aatttcattt tttcgacaaa caatgaatca
    26761 aacaaaaaat gagatcaatc aattgcaaat gaatatgcag cagacagcca aacttaaagc
    26821 aatcgatctc cgccatgatc cagtcaaagc agcgaaagct ttattcaaag aattggtgaa
    26881 agaaccaaac cgactgcatg aagcaagtca gtttctttat acgcatctgc cgaatctagt
    26941 tgatttgaca aataagtata atgagatcaa tgaccatgag atcaaaaaca aacagaccta
    27001 tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg tcagctttaa tcgcacaaga
    27061 ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac gtagagatat ctgttgcgaa
    27121 acagagctta aagcgtgata ataaatatga accagaacaa aaggattaac tcttttgttc
    27181 ataaaagaag actcggaaca ttcagatgat gtctggctgt ttcgagtact ttttctaaag
    27241 tagagacatt catgtaagaa tcgagaacag attcaacaag gaggaatttt ttatggatca
    27301 taaaccgact gaaacaaaag atgttacacc agttaacgat acacttgaag atttattgaa
    27361 caacccgttt tcaactccaa cagatacgct gaccacttca caacaagcgg agattgatca
    27421 gctccaagag caacagacag ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga
    27481 acaagctaaa cagctagcag caaaaatcga tgctaatgat gcacagtctg tcatcagcta
    27541 cggatcggct gcccaagcaa aattaagtga attctcacaa tcgatgttga atcatgtgca
    27601 agcacaagat attggtcctg taggagattc cttaactgaa ctgatgtatc gtctccaaga
    27661 agccaatcct gacgaactgc gtgccggaga agggaacatc ttccagcgtg tcttcgggaa
    27721 ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa aagattggtg cacaaattga
    27781 caagatttca gtgaaattag ataaagaaaa agacggtctg ctaaaagata atctgatgtt
    27841 agaacagctt taccagaaaa acaaagacta ctttgatgca ctgaatattt atatcgctgc
    27901 tggagaattg aaaatggaag aacttcaaac aacgatcatt ccagaagcga tgaaaagagc
    27961 agaagaaact ggcgatcaga tggatgttca gattgccaat gattacacgc aattcctaga
    28021 tcgtctggat aaacgtacgc atgatcttcg cttagctaga cagatcacga tccagcaagc
    28081 accacaaatc cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc
    28141 tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg
    28201 tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa acaacaaacg acttattgaa
    28261 gaaaaattca gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg
    28321 aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga
    28381 gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg
    28441 acacatggaa gaagatctga aacaaaaact gttggattta acccaataga tcacctttcg
    28501 cttgttacta aaacaaacgc ccaacaacag tttattacaa ctgttgatgg gcgtttttct
    28561 tatctttctt ctaacttctc cattggagtc tatttctcat tcatcttgct gtgtccatag
    28621 ccatagccaa aataaatgat cagtccaata accattacga taccgaacac gatccatgtc
    28681 acagtttgca gacgaagcat caatacaata caagtcaaga tggagacgat tggcaatagc
    28741 ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacatttt tctcaacttc
    28801 agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt tagttccgct
    28861 aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc ggcataagtc
    28921 gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg aagtaagcca
    28981 tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat cgttaccgtc
    29041 agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg tcctacatac
    29101 ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac gattcctgtc
    29161 aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc aatgattcct
    29221 cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac ggcatcaaat
    29281 cccaagaaag caaaaaacac aagagctgca ccatctagca caccggaaaa accaaaaggc
    29341 atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat aaacaacgcg
    29401 atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt cttggcttca
    29461 agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc aatgtatgta
    29521 ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc taaacttttc
    29581 aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc tagtaaaaat
    29641 tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac aacatacaga
    29701 taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa acttagtcca
    29761 gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg accagcattt
    29821 tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc accaattcca
    29881 agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg attgagattg
    29941 atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt ggaaaacagc
    30001 gattaaactg ttttcatata ccctaaaagg ataccaccta aaatgactaa aaggcaacca
    30061 acaataacat aaatgagctc ttttttcgtt tttctttcgc caaggatgaa aatcccacct
    30121 aaagtagaga taatgatccc catttgtgaa agtgagaagc caacagctaa accaagtgat
    30181 ttgactgtta gaagcatgca gatgtttcct aagccccata ataatccaca gatcatgttt
    30241 ttccacacaa actgatcgac tttaacttta cggaaagcaa agaagcttgc gcctaagatc
    30301 attccgatac tttgaggtaa gataattgac atcgcatcca aattcgccca gttgacgatg
    30361 atggtatata caccataacc aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa
    30421 tcaagcattt tattatctgt atctattttt ccttcgtcat cttgtcgggc agtcaaatac
    30481 gcaccgctta caagaagagc taaagcaata ataccgtata cataatcttt cgtttgtgtc
    30541 cattcatgga agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa accagtagac
    30601 aatggtagcc ctacagaaac acccatgtac ttcattccat ggaactgtcc attttgtcca
    30661 acactccaag ctaagccgga taaaaatccg atcaagaaaa tccaaggggt gattacagga
    30721 ctaacaacga aaaagacaac taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt
    30781 tgttgattgg catcgccacc aattttacca cttactaaac caatactacc ccatgccaac
    30841 atagggacta atgctattaa aatttccata accaatctcc ttttatttca catgataaga
    30901 aacttatcag cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa cgattttcta
    30961 acgtttttca ttataaaaat ttaagattgg gaaaaataaa gtcattgttg tcccttttcc
    31021 ttcagttgat tcagccgtca aatggatacc taatttttcg gccagatttt ttgctaaata
    31081 aagtcctaat cctgttgaat gtgttttgct aagtcgacca tttctcccag taaatccctt
    31141 atcaaagatc cgtttcaagt cttccgctgg gataccgatc cctgaatctt taactgaaag
    31201 gctgactcct tgcgcttcct tttctatgct cacaatgatg tttccgtgat ccggcgtata
    31261 tttgatggca ttactaagca attggttgaa gataaaagcg atccatttag catcggtcaa
    31321 aacagtatga tcctcaccaa cgatctcgta gcgaatattt ttttggataa aataatttgc
    31381 ctgactgcgt atcacaggct gaatgattcc ttttagtcca tattcttgga tgaggtagtc
    31441 ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt tctacgtatt catcgatttt
    31501 tgataattca ttttctagta atgtatactt ttgatcgtca atatcgaact cgatcgaacg
    31561 aactagcaat ttagaagcgg aaagtggcac tttgatttca tggacccatg agtcaatata
    31621 atctttttgg tcttgttgat tactgattgc tgattgcatt acttcttggt gttcacggat
    31681 cagctgattg atatattctt ctaccagttt ttcttcttct gtcctcccac cactcaaata
    31741 attttgcaaa ggggaagttt tttgtatgtc ggctaatttt ccccaccatc ttctcttgct
    31801 caagtaacca cccagcagat aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc
    31861 aattgtgctc aaatcaactt tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc
    31921 aataaaaaac agccatccca agatcaatgt ccactgatct ttcaaatatt taaaaaaatt
    31981 catcttaacc gctcatcctt tcagggaacg atatatccct gtcctacttt tgtttcgata
    32041 tatccttgga tacctgcctg ttcgattttt cggcgtaacc gattgatgtt gactgtcaat
    32101 gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt
    32161 aagatctttc cattgtgctt catcaaaata aataatagac gatattcatt tttgctcaaa
    32221 tcaatgatct ctccgttgat ttccatactt ccgttgtcaa cattcaacgt aatgccattg
    32281 tggctcatca tttcactaga taattctgtg tagttgtaag aacggcgaag taatgcattg
    32341 atttttgcaa tcagtacatc gatttggaaa tgttttgtca caaaatcatc tgcccccatg
    32401 ttcatagcca tgatcatatc catattcgta ttcctgctgg agataaaaat gatcggtact
    32461 ttcgatactt cacgtatttt ttgacaccaa taatagccat ccaaaacggg aagattaata
    32521 tctagtaata caagctgtgg ttcttctctt tcaaagtctg agaacacttg attgaaatcg
    32581 gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg
    32641 atctcttcgt cttcgactac catgattttt gccataaaaa atccctccat ctttcttctt
    32701 gctactacaa tatcaaagaa gcccttgtga aacaagttgg cttacctgga aaatgtcatt
    32761 ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc
    32821 agcttaaatt tattatgtta ttcttaataa ttggtctatt atctgttatt tttctttcgt
    32881 tgtttttgtc ttttaaattt tcgataacac caataaccga aaagcgttag cactagcaat
    32941 atacctagaa ggagtataag taatcgataa agatggtatt tttttgtctg ttctatcttt
    33001 gaagagactt cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc
    33061 acggtgtaag gtgtacaagt gataagtgtg actagatcct gcttttctct aattgctaat
    33121 gtatttgttt ttgttggaag gacaacaatt ttctcaacga cttcataagc gagcatccga
    33181 ccatacactt caagatagaa tcggtcatct tgctccattt cttcaagatc cgtaaagagt
    33241 tttttctcag caataccgct atgggcggta atcacggaat gtgtactctc tcctccagtt
    33301 ggaaaagagg tgccttgcag gactgtcgct cctctatcta acaaaagatc attcgtttca
    33361 tcaaataaag gcaagctaac attgattttc ggaatataga tggctccgat catatgttgt
    33421 tcgtaatatt cttttcctgg attttggaca tttctaaccg cttgctcaaa aggatctttg
    33481 acttgtccca ttcctgggat tactgatgta tgagcaagcg tctgattttt cttttcatgc
    33541 actgctaatt gttgctcatg tttttcttct ttatcgcgtt caagctgctt ttgataattc
    33601 tcgatccttc tttgatccaa ataattgctg agagcatctg ctaaaaatgg atatgtaaaa
    33661 actgcagctc cggcaagaaa catcactgcc ataaataatt gtaataaaag actgatttgg
    33721 gtttgaggtc tattacctat tttctttttc attttcttca ccttcttatt taaaaaaact
    33781 gcagcaacct ttataaaacc gattgctgca gcttattttt gttgcttata cttcagactt
    33841 cattttagat ttacggtacc agctgtacgc tccaatcatc aaacttattc cgattagtaa
    33901 gaatgccaag agcccgttcc caccagtagc aggaagtgac ccgcgtttca tattcggtac
    33961 tctttctggc cctggatttt ctactcctac gatctgaatt tcttcattat agcttccata
    34021 agagatgata aattcagtaa aggctgcctc acctgttgga agtacataac cttctggcgc
    34081 catggtctct ttcatttgat aggttccata atctaaccct tgaacctcaa agtatccatt
    34141 tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc
    34201 tgattcataa acagctaaag gcgactcccc tttataaaat attgcgtatt cagaacggtc
    34261 ttcattccac aattcgaatc ttgcatcttt aagcaattca ctgcttgatg catcatattt
    34321 ttcaaacttt cttccacctg ttgtgacttt ctctggcggc tctactcctt cttcataatc
    34381 aaattcatca tttcgtccga tttcaataac cgctgtgttg ttatggaaat catccgcttc
    34441 tagttctgtg gaaaccttca aattatacgt gatcgttaaa tctcctccac gtgcatctgc
    34501 taatctacct aaagtatcta ctttggctac tccattttct tctgtcaaaa tcgtcacgac
    34561 aaacccgttg cttactactt ctactatata atcttgtcct tttgttaaaa tcgtctcgcc
    34621 ggctcgcact tctatttcct gatcataata agttagacca actgtaggcg tatctgtgat
    34681 tttgtaattt ttgataacag cgcttccgtt ttcaaggaca gaatcgatga aataaggaat
    34741 aggtgctgtg atcgtgtaag aagccgtttt tcctctttct agatcaatat aagaaatcgt
    34801 ttcttgttct ccagcttcat taataatcgt tacttgtcta aggtcgcttt cctctacatt
    34861 catctgttta tctgcttctc tgatttcgtt tttcggatac aaatgcacat ctgtattgta
    34921 tgtgtctttc catgtagaac catcccacac gccatccacc atatcaggca tcatcactgg
    34981 catcataaca accattggag acgcgattct ggaaatatga gctggcgaat aagtttctaa
    35041 gaaaagatag actttatctc tagaagaagg tgcttcttcc gtcacttgga cggtagagaa
    35101 taccgcttct ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc gatattttaa
    35161 aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag aattaagacc
    35221 gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa tcgtaatata cttccgttac
    35281 gtcaaacaaa tcaaaatcga ctcctggaat attttcttca ccaaaatccg tagaaaccaa
    35341 tccgctattc ggtcgttctt ccggaacaga tgagaaacct tttttgtgta atgtaatcgt
    35401 cacttcactt ggtttttgtg cgacttcttc ggctttgact agttttccgt tcccgaaaat
    35461 ccctattccc gcagtaacga gtaacagtac actcattccc gtgacaatca tttttttatt
    35521 catttttttc tccccgttct ttttttatta ataaaacagc acctaacaat aaaagaattc
    35581 ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg gaatacggac cattttcctg
    35641 attgacctgg attgcttgga ccactcggct ctttcggttt gtccgttgtt tcttttacgg
    35701 tctcgttata aacacgtggt gttgcggatt cataggcaga gactggaact ttcccggatt
    35761 ctaattcagc catctcctgt ccgttaacta ctacatattg tggttggcct tttgcatcca
    35821 cccaaaattc agggatgatg acctcgattc tcttacttgc ttcatccatc tcgtaacctt
    35881 ccacaccttt taattcttca aaataatagg tgccagaagg caagaaacgt tgtcctgtcg
    35941 taacaaggcc ctctttatcc gaaatgaatt tgcttacatt tttgtcattc aaaggatcat
    36001 tgtctgctgg tttgatccat tgatttttca agtcattagc aggagccata tctaaataat
    36061 atttcccccc ttctatcatc tgatatagag caaaaacagc accttctaat ggttttcctt
    36121 tttcagttgt tcctctttga cgtccatact tgaagaagta tggatctctt agataaccga
    36181 catttttagg atagatatga atctcttgca gttcctcggt tgattctgtc gggtgataaa
    36241 ttggcaaaat agcagcaatt gggatagctg tctgatcgat atcaacatcc aatccaactt
    36301 ctggatcaat cgattcttcg aaaatcaaat aaactgcatc ccgatcttgg tttttcttag
    36361 gtgctttgat acgtgcaatc ccgtctccta atccgccaac cgtatctttc ccagttgtta
    36421 ccgaagtccc tatttggaca agcctttctt ctgtgattag ctgacgaata ttgtttctgt
    36481 tcgtttcagc gatttgctgg acgaattctt taggagtcat gttttcagca gctttttttt
    36541 catgataata atcggtcatg tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct
    36601 gtgaaatgat atctgctgtt tcccctgctt cctcagagat agccaagcca tcattttcat
    36661 ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat ccgtttatgc aacacgaatt
    36721 ccacgacatc ttcccctgat tcttctgccg acatcgcatg tggaagaagg actgctgcaa
    36781 gcggtagtaa aacaagtaat tgaagcatca aacacaacag ctttttcatt accttgctcc
    36841 ctccttttta gattgtctga caaacaaacc ataggctgct cctgcaagcg ccatgaagaa
    36901 tgcagcaagc aggatataga ataatcgtcc ggatcctcct gtagcaggca acggattttc
    36961 aggatcgttc ccgacactaa gctgtaatct gattcgattt gtctccaaaa gttcatattc
    37021 taactcttgc tgtagatcag gcgtctctgc ttcttcgttt ggataatacg aaatctcaac
    37081 ttttagtgta cccgcttctt ttcctggaac tacccgatca ttttctatgt caggttcttc
    37141 tcgataacta atttctaaaa taaagtaacc tggaagtttt ctatagctgt caggtgactt
    37201 cacttccttg atccgataga ccccttccaa caagtctgaa gcattgaata gggaagaatc
    37261 gtttggatct ggtgccaagc ctccctcgat gatttcatac tctcctgcac tattttgttt
    37321 ctcaattgaa aattctgcgc cttcaagacg tcggctattc atactgtcaa ttttcaaaag
    37381 ttccaaatcg attggcgcta gttcatcttc taaggttact tgtagtacac catcgatgat
    37441 ttcatagcct tccggcaagg gagcagtttc gtcttcgatg accgtatctt caatgataaa
    37501 cttaccttct tcattcagtg caaactggaa cacttgatca gatagccgat acccatttgg
    37561 tgcgacagat tccgagacct catattcacc aatagacaga tgcagaggtt ggtcgtaatt
    37621 taaatcgaca tacttttcta ctcctacggc acgagaatag aagtatccga tcccaggcaa
    37681 acgtttcgat tcttctggtt ctaacggatt accagtgtat gctcgataca gcgggaagga
    37741 agaatccccc actttcctga tatcgaaacc tgcaccatct aatgtatggc cttcatggtc
    37801 taattttcta actcggatct cccgataatc aaacgtattg taagcagtga atgctaccca
    37861 taaacgatcc tcttcatctg ttgtatacgt gagatattgc caattttctt cacctgtttg
    37921 cttgagttgg atagtcccta tagcattatc gtaaagtcgc aagatccatg tgtcattttc
    37981 atcaggttct ttgaaacctt ctgggttctg gctctcaatg attttgtatt ctgcatagtt
    38041 ggtagtttct tcactatcct tataaacaaa tgaatctggc tctccgattg gatgaataac
    38101 tttctcacca tccacgatta catatttccc gtcttcatct actgaataga acgtgagtct
    38161 ccctttctct tcctctactt cgttcacacc agaaagccct ttcgctactg gatatccgat
    38221 ttccagtccg ttaccatctg tttcataaag ttcaaactct gcgccattga ttggcatatt
    38281 actgccttca attagtttat tgatattcat gtggattggc ttgaattcat ttcgcatttc
    38341 cagtttgaca tacaactcat catactctgt gccttctatg ttaggctctt gtacaacaga
    38401 aagcttgtga tattctcctt cataatagga attcggatct ttaccttccg gtccgccgac
    38461 tctcgaacca gatgtatatt catctacaag ctcgtagtga cctaacatct catcttcgtt
    38521 ggctgcatat tttttgattt ggaattgata cgtctcatct actaactgat aacgtggtgg
    38581 tccagcgatc tctctgatcc aaaatatgtc gctgactgct ccagtttcat ctcctaaaat
    38641 cggcatcttc gtttcaaaga tcgctattcc gtccgtggta gttttacttg tttcgattgg
    38701 ttcggcttcc ccactctctg gattttggtc atataatgca aattcgactc cagccaacgg
    38761 ctcgccattt tgacccttct tgctgacttg gaaattacgg cctttatgtt cgtaacgaat
    38821 ggaagaagat gggaaataca tccgtccagt agtgatatca ttctcataat cctccaggtt
    38881 ctgaatatag gccatattgt tcactgtgca aaaatttccg tcttgagctc ccgcatcaag
    38941 ttttacttgg tacttgatcc tgaccatttc cccttcacct aaagaaatcg gagaaaattt
    39001 ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc gccactactt ctggtggggc
    39061 ttgtccatga cgttcttcat cccatatttc tggctcttgg tacttcatgc cgttttctcc
    39121 tggatctaga tggtaaaact gaatatctgc cgatctgcca gctccaaatg gtgagacatt
    39181 aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat
    39241 atcctccaag gctgtaacaa tgtcattctt gttgttcgca ttgtaatatc gcggtgttac
    39301 gccatcttct tctaaatcag caatattttt caatacattt cgtccaatag catctagtcg
    39361 ttgtctcccg ctaacgtttc catcgattcc taaaccaatg gagaagatac ttggcgggtt
    39421 ctcttgatac ttgtttttca gccaatgatg ataagcaact gtgttgatcg cagatgactt
    39481 ttcttgcgaa gtaggactgc cattcgtatt gttattagaa gaaccatcac caacccagtg
    39541 agtcgcttta ttatctcctt cttttacctc tccataacga tagtgtacat ttacttcatc
    39601 ttctggacgg ttaaagtcag atgcataggt aactggatga gtaaaccctg cattagaagt
    39661 attcccagaa aactcctcaa aatttctgaa caaaacgtca ttatcttcag caatcttatc
    39721 ttccatcaca gtccagttgc tccaagctcc attagtgctc gtacgataac ttgactgaat
    39781 cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg attaggattt tttctggatt
    39841 tctattttct ccgccattgt ctttatacaa tgtttcatag ccatttttaa gccctagtgt
    39901 aagcggagtt cctccgattg gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg
    39961 attgatatca tttgtcaatg gaaagatgtc cgaactgatt tgctcatggt tattgatagc
    40021 tgtactatta tggtaaaaat tcaccatacc gattcgaata tccagactcg ggtcagagag
    40081 cagatcttta ctcatttcgt ttactgcctc aatcaacgca gcatttttag tttgactatt
    40141 ggtcccagcg gttaattcac tcatactcgc tgacttatct agaacaaaaa cgatatcgat
    40201 tggactgatt tcctttttct cgcccccgat gacatctaga tagacattgt aaagggttgt
    40261 tgcatcttca ggatgtagat cagatgctgt gctgcttggc agaacaggtt ctacccattt
    40321 tttgatgtac gccccgttat aatccaagta actgttttca aaattccgtt catttcccca
    40381 aagctgaacg gtttgagctg gaacgtttct atcttcttcg cctgcagttg atccattatt
    40441 agtagcatag tcgtaatttc gccagttcgc tcccccgttt gtataatcac tatcatcgat
    40501 caagttctct ccgtcatatg gcgagccttt gtctccttga tagcgttcat tgctttgtgt
    40561 ccaatttcct aacacttttg tatagtcatt cgttgtaaac ttcatctatt ctttcatcaa
    40621 ccaccacttg aagaggtagt tacccttcgt tcacatcggg gatcttttct gtttcaaaaa
    40681 tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc gacataccat tcttgaccat
    40741 cgatcgtctc gacttttagg tcttctccat cttcatcata ggaggataga cctgttccac
    40801 gaacgttttt tacggtacca atcccggcgg ctgccgcatc gattgctaaa cgtattctac
    40861 cttcattggc actttccttt ttttcaaaag ccagatgcca ttcaagggtt tcttcttttt
    40921 caatatactt cccttcaatt tttgcatagt cctcatctaa gaatacctca tcctcctttg
    40981 tttctgttga ctcagcagct acatccaggg tcgtcatatg atagactgac tggaaatatg
    41041 gtccaaatag caaactgaca gttactaact gcaaaagctt caattttaac cctttcacat
    41101 ccttatactc ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa acaatgccca
    41161 tctaattata atttaattta attttaattt taaatcaaat gatatccttt ttagttaaaa
    41221 aac
//
LOCUS       DACEUF010000016        39221 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.017, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000016 DACEUF010000000
VERSION     DACEUF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.017"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(55..402)
                     /locus_tag="IXG20_000817"
     CDS             complement(55..402)
                     /locus_tag="IXG20_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609031.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKRLFVKSCW"
     gene            complement(395..697)
                     /locus_tag="IXG20_000818"
     CDS             complement(395..697)
                     /locus_tag="IXG20_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609032.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            complement(913..2304)
                     /locus_tag="IXG20_000819"
     CDS             complement(913..2304)
                     /locus_tag="IXG20_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1609033.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDNSEE
                     ALQEMNLVRTRMNKSEDIAQAFHVIHSFDKNISKELSIEKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNIDMETCKRLRIDPKDLERMRKINDEVLVENGLEPVDNQY
                     YENLEKRTNLYSNDPSKLKNGKINYQEFVQNAIEATLENCEVNSFVRFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRDELITLGREKQSRKEKSQYVERTFSARKLGK
                     KYSFESVFERLEKNLEETHMNNFVDLDTEQIIALASGVDYKTGELNVPFVELDSIAQK
                     QFSDHEGAKKIIKYDDFLREVDRHYANTISLEDDQEYTIKLHTDVNPRTLRSTKSKTI
                     LVKKTDQKELFLPAEGFKNCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQRRQRDFEMDF"
     gene            complement(2289..2900)
                     /locus_tag="IXG20_000820"
     CDS             complement(2289..2900)
                     /locus_tag="IXG20_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ1609034.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(2912..3100)
                     /locus_tag="IXG20_000821"
     CDS             complement(2912..3100)
                     /locus_tag="IXG20_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609035.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            complement(3114..5759)
                     /locus_tag="IXG20_000822"
     CDS             complement(3114..5759)
                     /locus_tag="IXG20_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609036.1"
                     /translation="MKIIKKKIANRKTNWLPVIFLVLIAVILFTSNIAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQAVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVEGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVA
                     YVCAVIVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVYAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEVLINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENLQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVYVRENVQSSGTSQPRTVDV
                     RENLQNTTSSQNGNRTVDVRENVQSSGTSQPRTVDVRENLQNTTSSQNGNRTVDVREN
                     VQSSASNQPRTVEVRETRAEASQGDKTQKINIIEERKERPNEKVIVEQEVKEKKRPRF
                     TYENNPLFQDISDKPNPLFDSLFKK"
     gene            complement(5746..5964)
                     /locus_tag="IXG20_000823"
     CDS             complement(5746..5964)
                     /locus_tag="IXG20_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609037.1"
                     /translation="MIENHLVDKIHCLDQNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            complement(5966..7009)
                     /locus_tag="IXG20_000824"
     CDS             complement(5966..7009)
                     /locus_tag="IXG20_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1609038.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKNKMQMFPNFYYLLKPVLCKDDVYKINKITHQENSQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLFESLS
                     TKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSDYRQFDNWYKKFNYAIKSAE
                     KEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAKTDHRIKQKNNYIFTSLKNK
                     ALELIKLQKKEGI"
     gene            complement(7164..7490)
                     /locus_tag="IXG20_000825"
     CDS             complement(7164..7490)
                     /locus_tag="IXG20_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609039.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(7498..7701)
                     /locus_tag="IXG20_000826"
     CDS             complement(7498..7701)
                     /locus_tag="IXG20_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1609040.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKSK"
     gene            7776..8426
                     /locus_tag="IXG20_000827"
     CDS             7776..8426
                     /locus_tag="IXG20_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1609041.1"
                     /translation="MSIKTLLNLWFLCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            8481..9647
                     /locus_tag="IXG20_000828"
     CDS             8481..9647
                     /locus_tag="IXG20_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1609042.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(9736..10128)
                     /gene="rpsI"
                     /locus_tag="IXG20_000829"
     CDS             complement(9736..10128)
                     /gene="rpsI"
                     /locus_tag="IXG20_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1609043.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(10144..10587)
                     /gene="rplM"
                     /locus_tag="IXG20_000830"
     CDS             complement(10144..10587)
                     /gene="rplM"
                     /locus_tag="IXG20_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ1609044.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(11106..11573)
                     /locus_tag="IXG20_000831"
     CDS             complement(11106..11573)
                     /locus_tag="IXG20_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1609045.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(11693..13231)
                     /locus_tag="IXG20_000832"
     CDS             complement(11693..13231)
                     /locus_tag="IXG20_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ1609046.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            13311..13997
                     /locus_tag="IXG20_000833"
     CDS             13311..13997
                     /locus_tag="IXG20_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ1609047.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(14198..14725)
                     /gene="msrA"
                     /locus_tag="IXG20_000834"
     CDS             complement(14198..14725)
                     /gene="msrA"
                     /locus_tag="IXG20_000834"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1609048.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14809..15486)
                     /gene="pgmB"
                     /locus_tag="IXG20_000835"
     CDS             complement(14809..15486)
                     /gene="pgmB"
                     /locus_tag="IXG20_000835"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1609049.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(15483..16613)
                     /locus_tag="IXG20_000836"
     CDS             complement(15483..16613)
                     /locus_tag="IXG20_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1609050.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(16613..18796)
                     /locus_tag="IXG20_000837"
     CDS             complement(16613..18796)
                     /locus_tag="IXG20_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1609051.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(18793..19818)
                     /locus_tag="IXG20_000838"
     CDS             complement(18793..19818)
                     /locus_tag="IXG20_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1609052.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(19863..20690)
                     /locus_tag="IXG20_000839"
     CDS             complement(19863..20690)
                     /locus_tag="IXG20_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1609053.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(20687..21754)
                     /locus_tag="IXG20_000840"
     CDS             complement(20687..21754)
                     /locus_tag="IXG20_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ1609054.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(21780..22616)
                     /locus_tag="IXG20_000841"
     CDS             complement(21780..22616)
                     /locus_tag="IXG20_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1609055.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(22613..23482)
                     /locus_tag="IXG20_000842"
     CDS             complement(22613..23482)
                     /locus_tag="IXG20_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1609056.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(23552..24835)
                     /locus_tag="IXG20_000843"
     CDS             complement(23552..24835)
                     /locus_tag="IXG20_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1609057.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(24849..26534)
                     /locus_tag="IXG20_000844"
     CDS             complement(24849..26534)
                     /locus_tag="IXG20_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ1609058.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            26809..27834
                     /locus_tag="IXG20_000845"
     CDS             26809..27834
                     /locus_tag="IXG20_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1609059.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(28429..32082)
                     /gene="rpoC"
                     /locus_tag="IXG20_000846"
     CDS             complement(28429..32082)
                     /gene="rpoC"
                     /locus_tag="IXG20_000846"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ1609060.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKSVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(32161..35778)
                     /gene="rpoB"
                     /locus_tag="IXG20_000847"
     CDS             complement(32161..35778)
                     /gene="rpoB"
                     /locus_tag="IXG20_000847"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1609061.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            36506..37474
                     /locus_tag="IXG20_000848"
     CDS             36506..37474
                     /locus_tag="IXG20_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1609062.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            37492..38397
                     /locus_tag="IXG20_000849"
     CDS             37492..38397
                     /locus_tag="IXG20_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1609063.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            38394..39200
                     /locus_tag="IXG20_000850"
     CDS             38394..39200
                     /locus_tag="IXG20_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1609064.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
BASE COUNT        11063 a         8238 c         6937 g        12983 t
ORIGIN      
        1 atgcggcgca cagaacgcgc cgcatatcgg actaagtttt attcaaattt gtcttcacca
       61 acacgatttg acgaagagcc ttttattttc aatcgaccat tgttgcggac gtatcggatt
      121 ttctgtatca tttttcattg tataggtcac ttgtaataca cgatcatgtt gcgcaaattc
      181 atttcttttt tcggtcaatt caaccttttt aaacgttatt tcgaccccat ctgcctgata
      241 agtttcaccc atttttaaat ctttagttac aatttttact ggtttttctt ctggaaaata
      301 tctggcttta attgttagat agcttccaaa aagaatcaat cctagtagca ataaaagaat
      361 tcctttaaag agccactgaa acggaaaata cactttaaac atctttcccc tcctccgttt
      421 tgcatttgtt ttttagataa tacgataact tttgatcaat ttcatccaac tttcccacca
      481 caattgattc tatttcagaa attttttgtt caattccttt gctttcagga ttacttaatt
      541 ttacatttga ttggttagct aacactggta aatttgatac catcacacga aacatattgg
      601 cttttgttcc gaaaatccct ttgttctgtt cgtaaaaact gtcaaaaatt gttttagttt
      661 cttttgaaca atacacttta atatacggat cagacatgtg tttttactcc ttatcttttt
      721 ttatttaggg agtctgtagg gagcttgctc cctcagaggt tttccccatc taacttgcca
      781 gaaagtagcc ctgcgtaagc aagggcggat tgactggcaa gtagatgggt agctggcgta
      841 tgccctcaaa accgcctctt tccaattccc tccgtatcga tgggaaactg gaaagtctac
      901 tggctaattc ctttagaaat ccatctcaaa atctcgttgt ctcctttgct gattttcaat
      961 catttgttgc gtgaactcgt aatctttttt agcacgatac attttcacat cgtcattcat
     1021 tatgcgactg atttgatagt gaatctgttt agcagaaggt gtatgagaac aatttttaaa
     1081 gccctcagct ggtagaaata attctttctg atctgtcttt ttaactaaaa ttgttttact
     1141 tttagtacta cgtaaagttc taggattcac atcagtatga agtttaatcg tgtattcttg
     1201 atcatcttct aaagatattg tgtttgcata atgcctgtct acttctcgca agaaatcatc
     1261 atatttaatg atttttttag caccctcatg atcactaaac tgcttttgtg caatggaatc
     1321 taattccaca aacggtacat ttaactcccc agttttataa tcaactccac tagctaacgc
     1381 tataatttgt tccgtatcta gatcaacaaa attattcata tgtgtttctt ctaaattctt
     1441 ttcaagtcgt tcaaaaacag attcaaatga atattttttc cctaactttc ttgcactaaa
     1501 agtacgttcg acatactgac ttttttcttt tctagattgt ttttctcttc ctaaagtaat
     1561 tagctcatct ctacgctcat agaaatttac atcttgtttg taaagaacat agccaagttt
     1621 gttatttgtt tttgaaaatt gataaacttc gatgttgtat tcttttgcta aatgatccga
     1681 aaaacgcaca aatgaattta cttcacaatt ctccaacgta gcttcaatcg cattttgtac
     1741 aaactcttga taattaattt ttccattttt cagtttactt ggatcgtttg aatagagatt
     1801 cgttctcttt tctaaatttt catagtactg attgtctaca ggttcgagtc cattttcaac
     1861 aagcacctca tcgttaattt ttcgcattcg ttctaaatct ttagggtcaa tacgcaacct
     1921 cttacaggtc tccatatcaa tattattaat aacaagatga ctatgaaaat gatctttatc
     1981 attgtgactt gcaacaataa tttgcgaatt tggaaaagct ttttctgcaa attcgacagc
     2041 tatttcgtgc attttctcaa tacttaactc tttagaaata ttcttatcaa acgaatggat
     2101 aacatgaaat gcttgtgcta tatcttcaga tttattcatc ctggttctta ctaaattcat
     2161 ttcttgcaat gcttcctcac tattatcact acaatttatt cctgagactg caacgcattt
     2221 ttcttttttt gaaggattag ctgcatattg taatagcgag gataaatttt tcttgcgtcc
     2281 tttcgatgtt atagtagctc ccaaagttgt ttcacctcat cttttaaact tgcaataatt
     2341 tgaatcgagt gaattaaatt ccggtcaact ttttgggaat actcttcttt cgtatcgggt
     2401 ctagtttgct tttctaacca atattgaaaa taatcttttt ctacttctga cagtttgaga
     2461 aaagaagatg tactccgatt ggactccaaa aaaatgtcat ctggataact aatcatcatt
     2521 ttttttgcta tgctattcgc aagttcatct ttaaaaatgt tgtaacggct aacgtcaaaa
     2581 ctaaaatgca ggctcttttc tttcttattt tcagattgtt ctctaaaata tttagcaatg
     2641 atatttaatg cttttaccgc ttgattcaaa ttgttaccaa tttgatttaa ctgtttgatt
     2701 agagctaccc tatcatcttc agccaataac ggcggttcat atttgagtcc ctttaaccgc
     2761 tcacgacaaa catctgctct agaaattcct ctttgttttg caatcaatgt cagttcaaga
     2821 taagtctttt tatcgacacg aaaatttaag gcctcactat gtttgcgatt tttttcgtac
     2881 attttttgtt tatcatccat tgattatccc ctcacatttt ttctagagag caacatttga
     2941 aattgctctc tatttcattt ttcatcatta attcttcttg aatcatcagt tcaatataat
     3001 ttgtgaccgt gattttttca tttgtcgctt tctttactga gtcgcataat tcggcaagtt
     3061 ctaagaaatc ctgcgtacta atctgtagaa ttaattgcat gacaatcacc tctttatttt
     3121 ttaaatagac tatcaaacag cggatttggt ttatcagaaa tatcctgaaa cagtggatta
     3181 ttttcataag taaatcgtgg acgtttcttc tccttcactt cttgttctac aatcactttt
     3241 tcgttaggcc tttcttttcg ttcctcgata atatttattt tttgcgtctt atctccctga
     3301 cttgcttcag ctcttgtttc acgtacttct accgttcttg gttgatttga cgcactactt
     3361 tgtacatttt ctcgtacatc tactgttcga tttccatttt gactacttgt agtattctgc
     3421 aagttttctc gtacatctac cgttctcggt tgacttgtcc cactactttg tacattttct
     3481 cgtacatcta ctgttcgatt tccattttga ctacttgtag tattctgcaa gttttctcgt
     3541 acatctaccg ttctcggttg gcttgtccca ctactttgta cattttctcg tacatatact
     3601 gttcgatttc cattttgact acttgtagta ttttgtaagt tttctcgaac atctactgag
     3661 cgcctatctt gactacttgt agaattttgt agattttctt tgactgattt ttctaatccg
     3721 gtatttgaat ttttagcttc ttggattctt tctttagtca acgcttcttt ttcgtccata
     3781 ttagcgcctg tacgcccttc tacacgtttc cttaacattt cctcacgatt cattgcttca
     3841 ggcgtgtatt ttgcttcctg tcgcttctga ttaattaata cttcagcaga cttagcttga
     3901 ccgtgcatag catttttacg ttcttcaata tcttgtttaa ctttttgctg aattgcactt
     3961 gcaggattaa tatttgattc agcattattt ttcattgctt cacgcatacg ttcagaacta
     4021 gacataattc tatcagcgtt ttcaacttta gcgccatctt ttctatccga aagacgtgtt
     4081 ggcatagaat cagcatattt tccagtttca tcttctttgt ttttagctac tataggtttg
     4141 ctatttttat taatagcgta cacggtacca tcatctaaac cttctttgaa tttattcttg
     4201 gtatcattag ccatatcacg catactctta aatggttttg ttgctctttc actggccatt
     4261 gtagctccat ccgaagctaa gcctgataat cctcgttcac gagcataccc aagtgttttc
     4321 cccgttttat tagctttttg agaaagtgat tgaccaaagt tgcgttcagg ttttcgttct
     4381 tccccttccg cttgatcgtt tccaccacta tttttcccac ctttagatcg gttcatacca
     4441 cctgttcctt tacgaaacag catagctccc atgccaaatg ctgaagcaag ctggccatag
     4501 ccttctttta aaccaatatc cacgccaaag taacgcatga ctgttccact accctttatc
     4561 agcacaataa cagcgcagac gtacgctact gcttttataa aaggatttac agaagaaccg
     4621 ctacctgttc cgagataatt aacaaacatt gcaaacatag acaatccgaa cccttgaaat
     4681 ccgactgtca aataacactg taaaatatct gacaaaactt gctttgatcg ttggcctgtt
     4741 tccaagtcag tagcaaatac taaaactcca agtacgcgtt taaacgctag ctcgagtatt
     4801 gctgtaatta taatgaaagc actaaaaaca tacgctaccg ctaaagcaat caatccaaaa
     4861 gaaatccccc atagatttgc ttgatagcga taatatcctg atttaatgcc ctcaacaaag
     4921 gtcgacagaa aattatccga aaatttggta gcaacaaatt cattatcaga attttcaact
     4981 aattcataac gcagattatc agctattggg ttctcttttt ccattttgtc gagtacatta
     5041 tctgtcaata cttgcgtaag atccgttttc caaaaatttt cttttgttag attgttccgg
     5101 tcattcgttt tatcgatcat ggaatattga tctgttttat tgatataagc taaatcagta
     5161 accccttgtt caatcagcga ccacgccacg ccttcgtccg aatttgtaat cgtagtcgta
     5221 tcattataaa atgttttaga aaactctatt ccgctgtgaa tcaacgttgg cattaaaaga
     5281 atcaatgaaa ttgacattac aacattcatt ccaacagatt ttagatcaac cgttccctta
     5341 cctgtaatca ttttataacc aataatcact agtgaaagta tcattaagcc aacaacaatg
     5401 gtacttaaaa cagcttgata gactttgttc aatccggtac tttctacaaa gctaaacaaa
     5461 tctaaaactt cgggaatcat attttgaatg ccatcgacaa cagcgaacat tgattttata
     5521 acagaccagc ctatccacat accgaactgg ctgtaaaaat ttccgttgta ttttttcaaa
     5581 taattctggt aaagcggatt agttaaaaac tcagtttgac tctcagtatc atcaaaccaa
     5641 ttgaaaatac tgccttctgc aatgttactc gtaaaaagaa tcacagctat tagaacaaga
     5701 aaaataaccg gtaaccaatt tgtttttcta ttagcaatct tttttttaat tattttcatt
     5761 gttacctccc tcagcccatt ttcttaggct ctcagagagt ttctgagcta tttttctgtc
     5821 aattatgata tgagttacgt aatcatcatt aacagctttt aaatcgagaa caatagtatt
     5881 tgagcagata ctctcggtta actcaagagt atctgcgttt tgatcaagac agtgaatctt
     5941 atctactaga tgattttcaa tcattttaaa ttccctcttt tttttgtagc ttgattagtt
     6001 ctaacgcttt gttttttaga gaagtaaaga tataattatt tttttgtttg atacgatgat
     6061 ctgtttttgc tcttcgtatc aagtcacgta aagtcattgc tagctgttct ctaacatgat
     6121 ctacaaaata actattatta caaatttcat agataatttt cagttgaact ttttgttctt
     6181 tttcagcaga tttaattgca taattgaatt ttttgtacca gttgtcgaat tgacgataat
     6241 cactattgct agccagtttt aagcaattca caatactttc tccccacatt tgtttaagaa
     6301 gattttcttc aataaatgtt tcgatcaatt cttcatctaa tttattattt ttagttgaaa
     6361 gtgattcaaa aagatcatca ttatgtgatt ctttaatatc tttactatct ttataagact
     6421 tatataattc tagttcattt tttgaactat cagcaaaatt gtttttgtct cttgttttca
     6481 ttgatgtgtc gcatagttca aattttgaac tatcaacaga atattgattt gttccatagt
     6541 tcaaattttg aactatcaag tttttttgac tattttcttg atgggttatc ttattaattt
     6601 tataaacatc atctttgcac agtacaggct ttaacaaata ataaaaattt gggaacattt
     6661 gcattttatt tttattgaag ccattttttc ttttcacaag aagtttaact ttttgaagtt
     6721 catctagtgc tgataagatt gtattttttg acattcccga aatttctacg agatttttga
     6781 tttcaaatat caaaaaaatt ttttgattct catcatacaa gttatttttc aatgaaagtc
     6841 tatttctatc ttttagtaac atataaacta acttagaact tagtttcatt ttcttatatt
     6901 tttcatttac gtttaaaaaa ataggatatt gatagtataa ttctgcatat atatcagaat
     6961 tagaatagcc ttgcttttca actaagcttt caacttcttt ttcattcatt gtcacacctg
     7021 cttctgattt tatttgctat aatagattca aataaagttt tctcagaact ttattattga
     7081 gtgttccccc gatctgcaaa ctgacgggaa cactcttttt aattttttct acttcatcat
     7141 tattcaaaaa aacacctcgt tttttaaatt tgatgactca atatccattt tttgcattct
     7201 tcttttgaaa acaactccat tttttctaat aaaataactg gcaatcccat tcttttaaat
     7261 ttcaaaaaag ttgtatatga tacattcaaa tatttagcca tctcttttcc ttttagaaat
     7321 ggtctttcta acccggtatc aattcttgct tgatcaatag cctcttttgt catttcaaaa
     7381 acataatttt tcaattgatt cgtttgttgt ttatctaaca ttactgtaat tcctgcatcc
     7441 attatttttc tcctctcatt ttctatttct tcaatcagcc aactcgccaa gttgtattta
     7501 tttagatttc aaatcattaa gtaaccaatt taccaacttt tgataagtag atgctctaat
     7561 actttctttg ctcccatttt cgattttgac atatgttttc cgatttatac ctatcaattt
     7621 tgctgcatcg tttttagtaa gtataagatc ggctctatgt cttcttaagc ttctaatgaa
     7681 ttgatcatta agagttatca ttcagcgcct ccttttaaat tattcacttt gtggttttta
     7741 tcttaataag cacattttaa accacgaaac gagtaatgtc aattaaaaca ttactcaatt
     7801 tgtggttttt atgctataat agtttggaat ttactatagg gggtgttact ttgaatagaa
     7861 taaaatttta tattgattcc agtaaaaaga ccattaaaca aattagtaca gaaactggta
     7921 ttaactatac aactcttacg aactattacc gtggtgagcg aaatcctaga aatactgaaa
     7981 catggaatat ccttgctgat tatttccatg ttcctgtaag ttatttgatg ggattagacg
     8041 aaacaaattc catagaacaa cctaatcttg atagaataaa aactgctcgt ttagaaaaaa
     8101 aggacaaaat agtcaagaat attgccagtc ttgactcaga ttattttact gaattatcta
     8161 atctatactt ttccatgact tctttacttg aagtcaaact tcaagaagat gactccgact
     8221 ttcttttttt aaaaagtatt ctagaggact cctttgaata cacgattcta tttgccaaac
     8281 aaaagaagtt tattaataaa gctttaaact taaatatcga tgaaagaaaa gagttagaat
     8341 ctgagttatt tggcaactta acccaagtac aggttaacaa tctaaaaaga gagataacag
     8401 ctcgattaat atcaaaactt caataaaact aattattcaa tcagccaact cgccaagtta
     8461 tcagattgga gagattagtt atgtcaatac aaaagtacac aacaaaaagc ggtgcacgat
     8521 atagtgtccg cttgtatgtt cgtaaagatg aaaatggaaa gcaagaatac tatgtaaaaa
     8581 aagggtttaa aactgaaaaa gaagccaagt tacacgaagc aagaaaaaag gtcgagattg
     8641 aagataccgg ctatcataag ccttctaatg acttgtttat aacagtgtat gaagaatggt
     8701 tgccttctta taggaatcgt gtacaagaaa cgacatatca acgtacaaaa gaccttttcc
     8761 gcctgcatat actaccaaaa ttcgggaata agtccatttc taagattact cctgttcact
     8821 gtcagaatgc agtcaatgaa tgggctttaa aattcaagaa tatcaaacag cttaagtctt
     8881 acagctctca aatatttgag tatgccattt ttgcagagct attacaccga aatcctatgg
     8941 caaatacaaa tctaccaaag cgggaaaaga aagaaaatga aaattacttt tcattagaag
     9001 aactaaaagc tttttgggat attctcaata aagaagagcc tttaaaacat attttaatct
     9061 tccagctttt gattactact ggtatacgta aaggagagct agctgctctt cattggtctg
     9121 acatttattt tgaagaacag ctaatgtatg ttagaaagag ctatgcaact attcgaaatg
     9181 aagagaagaa tgcaagaaga aaaactgtca ggattcaaaa aaatacaaaa aatatatctt
     9241 ccgaacgaat attaccaatt gatgcacaaa ctattgaaat gttaaaaatc tggaaaaaag
     9301 aacaagctcg agagcttttg cagtttggca taaatacaaa tacaaaaaat caacttatct
     9361 ttacctatgt aaatgcagac ggtgacatta atcaaccttt gcatgcggac tattcaaata
     9421 atatattgaa acgtttagag aagaaatata aatttaagca cgttactatt catggattcc
     9481 ggcacactca cgctactctt ctactagaag gtggagcaag tatcaaagaa acacaagatc
     9541 gattgggtca taaaaatgct gaaactaccc ttaacactta ttctcatgtt actgaaaaag
     9601 ctcaacgaaa tgcgttagaa aaattcattg cttacactgg tttataattt tgtcattacc
     9661 tttctcatta ccagaacgaa aaaaagacac cttctcgcaa tgagaaagtg tctgaaatcg
     9721 ttggtataac aacgattaac gttttgaaaa ctgtgaagct ttacgtgctt tcttaagacc
     9781 tggtttttta cgttcgacca tacgtgcgtc acgagtaagt aagccagcac gtttaagagc
     9841 taaacggaag tcaggatcaa cttctagcaa tgcacgagcg ataccatgac ggatagctcc
     9901 tgattgtcca gcgtatcctc cgccgtttac gttaacaaat acgtcatatg cgccttttgt
     9961 ttcagtaaca gcgaaaggtt gattgattac ttcacgcaag tcagcatgtg gaatgtattc
    10021 ttcaacatct tttttattta cagtaatttt gccagttcct ggtactagac gtacgcgagc
    10081 aactgcgttt ttacgacggc ctgtgccgat atattgaact tgtgccaatg aaattccctc
    10141 ctattagatt aagtttgtga tgtctaaaac ttctggttgt tgtgctgcat gtggatgttc
    10201 tgctccaccg tatacgttca attttgtaaa ttgtttgcgg cctaaagtgt ttttaggaag
    10261 catacctttt acagaagttt cgatcaaacg acgagaattt ttagcacgca attcgccagc
    10321 agtgattgat ttcaatcctc ctgggtagtt gctgtgacgg taataaattt tgtcagtcgc
    10381 ttttttacct gttaatttca ctttgtctgc attgatgacg atgacgaaat cgccagtatc
    10441 caaatgaggt gtgaaagttg gtttattttt accacgtagt acagatgcaa caactgttga
    10501 aagacgtccc aatggaacat cagttgcgtc tactacatac catttacgtt ctacttcgcc
    10561 tggtttggcc atatatgttg tacgcacgtt attttcctcc aatttaagtt ctgatgtttg
    10621 acatacactt cgagtttccg gggctctttg tggggcaaac aataccattt aacatattac
    10681 ccgctaatct agtcaatgtc aacgtttttc tgcttgattg aaaaatttat atgaaacttt
    10741 acttcttttc tttcttttat ataaaagggt cgtttcctgt caaaatgaaa aacaaaaaga
    10801 ggctgtgaca aaagtcttgt cacaacccct tattttgaat aaacggtgtt ctcctgccaa
    10861 ctcctcggtc acaagtcaca atatccataa aacataagaa gctgtttttt aaaattgttt
    10921 cttgtgactc ggagctgaac ggcaggctta caacctcttg gtaaacggtg ttcaatcagc
    10981 tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttg acgtttgttt
    11041 ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa gttcaacaac
    11101 agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat ccagatacgt
    11161 ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc gccttcttct
    11221 ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc tgcaacaagt
    11281 gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg gatttttgtg
    11341 ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat caagcgttcg
    11401 gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat tgggtgcaat
    11461 gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac agacatttgg
    11521 cttagatctg caactaattg atttaatact tctttggttt gttggaattt catctttctt
    11581 gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa agtacaacaa
    11641 aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc aatcattttt
    11701 cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt gctaaatgct
    11761 tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca gtttcttgta
    11821 ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt tggatctttc
    11881 cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg acagtaggga
    11941 aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga gattgagcaa
    12001 acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct tttaaaatcc
    12061 gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt tctgtatgtt
    12121 gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt gctcgctcac
    12181 caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa gcgatagccg
    12241 aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg acttcctgta
    12301 aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct cctcccacta
    12361 cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg aactgaggct
    12421 gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg atcgtatcac
    12481 caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa acaaatgata
    12541 tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt acttgatagg
    12601 tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca agcacttctt
    12661 ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt ccgtagtcgc
    12721 tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca aaataagcag
    12781 tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc aatggtgtca
    12841 ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg gtatatttca
    12901 gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga gataacggtg
    12961 ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag acaaattgaa
    13021 tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag acaccttgaa
    13081 agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa gggtagcgaa
    13141 aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg gtttgtgtgg
    13201 ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta tgtatgtaca
    13261 aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc gtgttacttt
    13321 attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag cggattccgt
    13381 taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt tcgctttctt
    13441 ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc cgctattgca
    13501 ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt ctttttgcct
    13561 ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg ctttcggctt
    13621 tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg ctattctgtt
    13681 cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg caaagtgtgc
    13741 tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat ctcggagctg
    13801 gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag ttgcttcaga
    13861 tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt atctgtcata
    13921 aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg ctgattgtcc
    13981 ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct ctgatatata
    14041 gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca acctctccac
    14101 taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt ttacaacctc
    14161 tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg ccccaatgtc
    14221 gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg agcttgtttt
    14281 tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt ggttcgattt
    14341 ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt gaacgctctg
    14401 caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa tcaccgcgat
    14461 cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt tccaccaact
    14521 gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta tgccctgttg
    14581 tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta taaccggatg
    14641 tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa aagcatccgc
    14701 ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca taccttgatt
    14761 tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt atatacgtga
    14821 tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta aatcaggaat
    14881 cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat gcatgttacc
    14941 agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg catttggcgg
    15001 tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg cttttgatac
    15061 ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt ctaaaatgag
    15121 gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt tcaagtactc
    15181 aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa tcgcttgaag
    15241 atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga atttctgttc
    15301 catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc ctttcagttg
    15361 ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca gccacgcttc
    15421 gtaatgataa cgagctgtgt ctacaagaac accatctaaa tcgaatagtg ctgctttgat
    15481 tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat gtttttagct
    15541 gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac agctgcagga
    15601 aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc tttaccaaca
    15661 cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat cccaactggc
    15721 gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac gaggtctgct
    15781 tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc agagactatt
    15841 ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact actgcccgat
    15901 gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg gtcagccact
    15961 gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc gccttctttt
    16021 ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat cagctcaata
    16081 ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag tgcttctact
    16141 ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc gatgccgcca
    16201 tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg ttgcaacaat
    16261 tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg ttggtttagt
    16321 gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg accgtctgaa
    16381 gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt ttgccgcttc
    16441 aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc tataggtagc
    16501 agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc ttgtgctgcc
    16561 tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg cattattttc
    16621 ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg acaaaaatag
    16681 gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa acaaatagca
    16741 acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa ggaagtcttg
    16801 gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat ataactgcta
    16861 gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc ggatctggtc
    16921 ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt tcttggatac
    16981 ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt gttttgtttt
    17041 catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt aataagtata
    17101 acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg ctacgggcat
    17161 aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa tcaataactg
    17221 gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc actggcaaat
    17281 ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca gcttgatatt
    17341 taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta ttgtcgatat
    17401 attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca tcgactttta
    17461 cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc tttgtttcta
    17521 aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca aggtctgcac
    17581 tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt ttgatattca
    17641 atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat gggaaaagtg
    17701 caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga tcataacggt
    17761 acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc aggataaaaa
    17821 tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc ttcgccccta
    17881 cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca gcgaagtcca
    17941 tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga tgccaaaaat
    18001 ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt ttctgggctt
    18061 gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc acgatgatct
    18121 tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg gtgatccggc
    18181 gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca atatcaattt
    18241 tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact cgcagttctt
    18301 tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc gttttcacat
    18361 ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc tgatggagat
    18421 tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga gcagtttcaa
    18481 tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg tatgcaagaa
    18541 ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat ctgatgacat
    18601 ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat acaccagctg
    18661 caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg ataccaaaaa
    18721 atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa tcaaactgtt
    18781 tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat ataaattttc
    18841 caccagattc tggatgacat atccttgcca gccatctgca atcatctgag tctggtcacc
    18901 gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa ctggcgtggt
    18961 cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg gatacagatt
    19021 agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga tattcaaagc
    19081 aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc caaaaattgc
    19141 atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat tttttttcgt
    19201 accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc cagtaatata
    19261 catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc cttgaagttc
    19321 tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat tcgcttctac
    19381 gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt cgtcagaaaa
    19441 acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct ctgccatctt
    19501 tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag cattagcttc
    19561 gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac tcacgatatc
    19621 gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa atcctgtacg
    19681 tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca attctaagac
    19741 atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc cagcgcctat
    19801 gattgctact ttcttcattt atttctccct cctttgctta tttacttatt gttattcttt
    19861 ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt tttcaagtat
    19921 tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca ttcatatacc
    19981 atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa atcaattgcg
    20041 ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac atactctttt
    20101 tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata aaaatcagcg
    20161 atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc cgctaacaaa
    20221 ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt acctgtttct
    20281 tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc tccttctttg
    20341 cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc tgctgggata
    20401 acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat ttgacgtaca
    20461 ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc gttacaagct
    20521 gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat attttctatt
    20581 aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa tctaaatttt
    20641 tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat tgttctcctc
    20701 ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca taacgcatgt
    20761 aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca caatccaagt
    20821 aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc catcttgggt
    20881 aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta ttgaaatgtg
    20941 cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca taagaaatga
    21001 tcccgccata gccgatccct cttaaagaag cttgtaatgc ggcagcactg ccactcgttt
    21061 caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa ccgacatctg
    21121 ctttcatagg atctagcgtg taatcggcac cgtatttttc tgctaattct ctacgctgcg
    21181 ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat tgaatcgcaa
    21241 tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga acatgtgctt
    21301 cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt ttccattgat
    21361 ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc agcgtttcac
    21421 gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc aaactgcccg
    21481 tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat accacaccgc
    21541 gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat tcttcatcga
    21601 taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga gtagcgaaaa
    21661 ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct ttgaatccgg
    21721 ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt cttttctttt
    21781 tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga taacgcatac
    21841 ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc ccaaatgtag
    21901 tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc ggaggtcgac
    21961 aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata aacgaagaca
    22021 gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg gatccggcta
    22081 agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa ataacttttt
    22141 agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag tgctgtattg
    22201 gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat caaaataccg
    22261 gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt gaagcgtagt
    22321 tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga ggctatcaag
    22381 gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc attgaaaata
    22441 tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt tgcaattgct
    22501 tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa attgacagct
    22561 aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt tttcatttca
    22621 agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac aatagaatcg
    22681 atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac aaataaacgc
    22741 ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag aacatccaga
    22801 tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt gtcacgatct
    22861 tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc gttttgtcga
    22921 ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc cagatagcaa
    22981 acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt gcttcgttga
    23041 aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta ttgaacatat
    23101 atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa atcagtgaac
    23161 gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct aatcctaaag
    23221 ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa gaacgataaa
    23281 attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg gcgtctgagt
    23341 taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt ggaatcagca
    23401 ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat tttaagtcag
    23461 aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat agctatctac
    23521 ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt gcgtcagcta
    23581 aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt ccgatcacac
    23641 ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt ccaaagactt
    23701 gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac ttgatgactt
    23761 cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga gcaccaccag
    23821 gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct gtgaatgctt
    23881 cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca gatactgtgc
    23941 catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct gagttccccg
    24001 cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct gttccattca
    24061 tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat tgggataagt
    24121 tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc tcgccttcat
    24181 catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc tcactcattg
    24241 ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct gtgaaatgtt
    24301 gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct tctgataatt
    24361 tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca atatagcttt
    24421 ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct tctccagctt
    24481 cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc atcactttag
    24541 caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct aacgtaacga
    24601 tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg tctggatttt
    24661 ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt tcgacagaag
    24721 agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt tcaccacaag
    24781 cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt ttcatttttt
    24841 tccacctctt attttattat tttcagccac atatattcat acggctggaa tatcaacttt
    24901 cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc tccttttggt
    24961 aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt ctcttccttc
    25021 gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat caaactattc
    25081 ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc accatatact
    25141 gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa aaatttttgc
    25201 cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt tcgacttccc
    25261 agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa tgaatgagca
    25321 gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa cgcatctaga
    25381 tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt gtagtttact
    25441 cgggcaccct ctttcttcag ctgttccact aattcaagga tcgttttttc cggcagaata
    25501 cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa aaaagttgtt
    25561 tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt attacccgaa
    25621 cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg tgcttcattc
    25681 tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc agtgatcaat
    25741 accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat tttatgcgtt
    25801 tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac agcgtccaag
    25861 cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat tttcaataaa
    25921 acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt ccatacgtgt
    25981 ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct tggtctagtc
    26041 acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta cgtatcattc
    26101 aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca gacaaagtcg
    26161 aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc caaacggtcg
    26221 ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg gaaaaatggc
    26281 aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt cttaaatgta
    26341 cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa gaaaatatct
    26401 tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac actgactttt
    26461 tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat cgcttgtaaa
    26521 agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg atttgatcat
    26581 attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata tacacaaaaa
    26641 tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat atggtctctc
    26701 atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa tgtctattgt
    26761 actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt gaaacctact
    26821 atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag agtattaaat
    26881 aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat caaagaattg
    26941 aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga cgcgattcta
    27001 gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc agatgttatt
    27061 caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca aacggcaaaa
    27121 acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat gatccgagga
    27181 attattattt taagctctac gatggatgaa acacttcttt ttgaattgga tcaatacaat
    27241 attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta ctcagtggac
    27301 acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa tggacaccgt
    27361 catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg tctcaatggt
    27421 taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat tatagacggc
    27481 ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct acgagaaaga
    27541 ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa agtggcagaa
    27601 caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga tcccttattg
    27661 tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca gttaggaatc
    27721 aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga aaaaagaatc
    27781 attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat gtagaacaga
    27841 attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt ttcaatcact
    27901 catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc atctgtttta
    27961 gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg gaagaaaaaa
    28021 ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac acatttaccc
    28081 ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag aataacagac
    28141 atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca aaatattaaa
    28201 aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc tatctttact
    28261 tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg atacgaaccg
    28321 atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt agacaaacca
    28381 aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct attttgtttg
    28441 attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac tgtagacatt
    28501 ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca taccagtacc
    28561 agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag gatctttctt
    28621 accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg cagcagataa
    28681 gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc gagaagttgc
    28741 aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg cgatgtctaa
    28801 taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt tacgcagcat
    28861 ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca tacggtaaac
    28921 acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa cttgcaacaa
    28981 ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca tatcgccttc
    29041 tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg tttttccttt
    29101 gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga catcccctgt
    29161 tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt cttggacacg
    29221 aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg tacgcatcgt
    29281 caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg cttctccgac
    29341 ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga caccatgttt
    29401 tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat caacgatttg
    29461 ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat ttgtttcagg
    29521 atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg attcgatgat
    29581 ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc aatcttcttc
    29641 acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac ctgaatcggc
    29701 agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga taaacatttc
    29761 caagacggat agtccttcac ggaagttcga aatgattggc aattccatga ttcgaccatt
    29821 aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa tgttaccacg
    29881 agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt ccatcagttt
    29941 ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat aacgttcgtc
    30001 atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt tatgggcatt
    30061 ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta ctgtgatacc
    30121 ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct tagaagtttc
    30181 tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt tcttcttgaa
    30241 cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag cttcaacaaa
    30301 gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca gataagggaa
    30361 ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca tcgtacgttc
    30421 tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac cgatacgtga
    30481 atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat ctctgaagac
    30541 cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac ctagaaccat
    30601 atcttgagaa ggagtaacaa ctgatttacc atctttaggg ttcaagatgt tttgagcagc
    30661 taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt ggacagccat
    30721 ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca aacggattgc
    30781 gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa gcgtaggtgc
    30841 ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc atacttcgtc
    30901 ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt cgcgttgaac
    30961 aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg gaagtccaca
    31021 ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt agtcgacacg
    31081 tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat gagaaagaga
    31141 tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt tatcgatcaa
    31201 tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc caggtgcatt
    31261 caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt aaagatcatt
    31321 caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta aatctggtgg
    31381 aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag cgcggaatgc
    31441 ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg cagatttcaa
    31501 ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta attgtttaat
    31561 cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt tttcgcgata
    31621 ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt cgatgactac
    31681 atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta atacaagacc
    31741 catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg ctaattcaat
    31801 atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc atcgatcaca
    31861 aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc agtcttttgt
    31921 aggaccaaaa atccgctcac agaacaaccc ttcgcgttca ggttttaagg tacgatagtt
    31981 aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt cgggagaagc
    32041 caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt ccctccattt
    32101 catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc atagaccgga
    32161 ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt tttcatcttc
    32221 cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg caaattttgt
    32281 caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa gttcgatctc
    32341 tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt ctttcaccaa
    32401 tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga cgattgcttc
    32461 gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt cttgtaaagt
    32521 ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac gttgtccacc
    32581 aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc cgattgaacg
    32641 tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca caccgacaga
    32701 cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt tcgcatcgct
    32761 ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac catcaaatac
    32821 tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta attccaatac
    32881 ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat cgattggtgt
    32941 tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa cccctttatt
    33001 tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa cgatataaac
    33061 acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag taaagatttt
    33121 cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat cgcggacttc
    33181 acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata attctgttac
    33241 cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg caccaatacg
    33301 gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag ggatttcgcg
    33361 tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt cttcaatatg
    33421 gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg catcttcata
    33481 gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag ccatttcgcc
    33541 ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct cacccaaatg
    33601 aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt ttgttacatc
    33661 gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat caacgtattc
    33721 aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg atttgtattc
    33781 cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt gacgctgcat
    33841 gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa tacatgctgt
    33901 cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat caatagatac
    33961 ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg ttccatcttc
    34021 attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag ctgtcaagta
    34081 gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag gtgtttcgat
    34141 aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc cgatgtttgg
    34201 gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa cgtcacgaac
    34261 ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa gacgacgttt
    34321 gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac tgtgacaact gagaagaacc
    34381 aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt gaggtgtcaa
    34441 tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac gtgctagccc
    34501 gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat ttcctaagtg
    34561 gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc tcattgaagc
    34621 aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt ttccgatcac
    34681 gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga cagtcattgg
    34741 atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc cattatcaat
    34801 gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt caacgatgat
    34861 ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata gacgtgtttt
    34921 caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt aacgttttgg
    34981 atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg gttcgcctgg
    35041 acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc ttgcgttttt
    35101 atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga tttcaaagat
    35161 cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa tcttacgtgt
    35221 acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta gccaagctcc
    35281 acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga cttttccatg
    35341 gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg ccccgttgat
    35401 gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt cttgtgcttt
    35461 gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg cagagtagtt
    35521 tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt cataatctac
    35581 aaattccaaa gatagattcc cattgaagtc atcgattggc aaaatgtctt caaacatttc
    35641 tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga tcaaatttgg
    35701 taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt ttccgtattt
    35761 tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa gactacccga
    35821 tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca ttttttgcgg
    35881 cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact tcgtgagctt
    35941 ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct gccttttctt
    36001 catcagatag tttttgcaac ttcatatact acacttattt tctataaaag tcaacacttc
    36061 ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt atgttttcaa
    36121 ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta gaaaattcat
    36181 tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca caactgaata
    36241 acttttactt tagctacttt agataggttt gtattcatca tgatggatac aaacgttaaa
    36301 cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag ccacgtagac
    36361 aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca ttctactttt
    36421 tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg attttttgtt
    36481 tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat taggcgctgc
    36541 cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg catcagatgg
    36601 acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg ctgcctataa
    36661 aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc taacaatcga
    36721 ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga aactcgtcaa
    36781 agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt tattaaatga
    36841 aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag cccatttagt
    36901 cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg tgccaatcga
    36961 aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg gcatcatgta
    37021 taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag aattgaaaaa
    37081 agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc aacaagtgac
    37141 gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca cgttcgcaag
    37201 tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg ttgctggcgc
    37261 agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg aaagcttggg
    37321 caaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa gtgaacttcc
    37381 tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca aagcattagg
    37441 catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct catgacaaat
    37501 ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct acttgcgatt
    37561 ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga aggaagcttt
    37621 cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc attgacagct
    37681 tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact gcttcacaca
    37741 aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt atattctgtc
    37801 acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac gatctttcat
    37861 tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg tctgcttttt
    37921 gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg acttgctatt
    37981 cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac agataatatg
    38041 aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc cttgcttgcg
    38101 caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat tggacttgct
    38161 tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg tctcttgact
    38221 gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga actgaatgtt
    38281 gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct ttctttcccg
    38341 cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt caaatgaatg
    38401 acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc aacgaaaatc
    38461 atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg atcatcggtg
    38521 gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt tctgtcgatc
    38581 agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc gaacgttcga
    38641 aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt ttaacggtag
    38701 aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc cgtggtgtca
    38761 aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg gggttggaaa
    38821 atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct attacgttat
    38881 tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact gcagcgcttg
    38941 accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact gagcagaatc
    39001 tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg agtcgactga
    39061 tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa cagcagctca
    39121 cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta aaagacgatc
    39181 aagtactttt atcttcataa tcagaaagag gatgggacaa a
//
LOCUS       DACEUF010000017        38274 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.018, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000017 DACEUF010000000
VERSION     DACEUF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.018"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(62..973)
                     /locus_tag="IXG20_000851"
     CDS             complement(62..973)
                     /locus_tag="IXG20_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1609065.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1430..1858)
                     /locus_tag="IXG20_000852"
     CDS             complement(1430..1858)
                     /locus_tag="IXG20_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609066.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(1886..2524)
                     /locus_tag="IXG20_000853"
     CDS             complement(1886..2524)
                     /locus_tag="IXG20_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609067.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2528..4564)
                     /locus_tag="IXG20_000854"
     CDS             complement(2528..4564)
                     /locus_tag="IXG20_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ1609068.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4690..5355
                     /locus_tag="IXG20_000855"
     CDS             4690..5355
                     /locus_tag="IXG20_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1609069.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(7290..10439)
                     /locus_tag="IXG20_000856"
     CDS             complement(7290..10439)
                     /locus_tag="IXG20_000856"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ1609070.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(10452..11651)
                     /locus_tag="IXG20_000857"
     CDS             complement(10452..11651)
                     /locus_tag="IXG20_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ1609071.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(11641..13236)
                     /locus_tag="IXG20_000858"
     CDS             complement(11641..13236)
                     /locus_tag="IXG20_000858"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1609072.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(13820..14527)
                     /locus_tag="IXG20_000859"
     CDS             complement(13820..14527)
                     /locus_tag="IXG20_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609073.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(14592..15428)
                     /locus_tag="IXG20_000860"
     CDS             complement(14592..15428)
                     /locus_tag="IXG20_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1609074.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(15446..16855)
                     /locus_tag="IXG20_000861"
     CDS             complement(15446..16855)
                     /locus_tag="IXG20_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1609075.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(16869..18692)
                     /locus_tag="IXG20_000862"
     CDS             complement(16869..18692)
                     /locus_tag="IXG20_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1609076.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(19535..19861)
                     /locus_tag="IXG20_000863"
     CDS             complement(19535..19861)
                     /locus_tag="IXG20_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609077.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(19907..20269)
                     /locus_tag="IXG20_000864"
     CDS             complement(19907..20269)
                     /locus_tag="IXG20_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ1609078.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(20306..20521)
                     /locus_tag="IXG20_000865"
     CDS             complement(20306..20521)
                     /locus_tag="IXG20_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1609079.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            20648..20983
                     /locus_tag="IXG20_000866"
     CDS             20648..20983
                     /locus_tag="IXG20_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1609080.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(21059..23056)
                     /locus_tag="IXG20_000867"
     CDS             complement(21059..23056)
                     /locus_tag="IXG20_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609081.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(23154..23429)
                     /locus_tag="IXG20_000868"
     CDS             complement(23154..23429)
                     /locus_tag="IXG20_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1609082.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            23661..24395
                     /locus_tag="IXG20_000869"
     CDS             23661..24395
                     /locus_tag="IXG20_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1609083.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            24434..25576
                     /locus_tag="IXG20_000870"
     CDS             24434..25576
                     /locus_tag="IXG20_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1609084.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(25671..27062)
                     /gene="sufB"
                     /locus_tag="IXG20_000871"
     CDS             complement(25671..27062)
                     /gene="sufB"
                     /locus_tag="IXG20_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ1609085.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(27067..27537)
                     /locus_tag="IXG20_000872"
     CDS             complement(27067..27537)
                     /locus_tag="IXG20_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ1609086.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(27524..28759)
                     /locus_tag="IXG20_000873"
     CDS             complement(27524..28759)
                     /locus_tag="IXG20_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1609087.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(28756..30042)
                     /gene="sufD"
                     /locus_tag="IXG20_000874"
     CDS             complement(28756..30042)
                     /gene="sufD"
                     /locus_tag="IXG20_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ1609088.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(30055..30828)
                     /gene="sufC"
                     /locus_tag="IXG20_000875"
     CDS             complement(30055..30828)
                     /gene="sufC"
                     /locus_tag="IXG20_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ1609089.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(31025..31873)
                     /locus_tag="IXG20_000876"
     CDS             complement(31025..31873)
                     /locus_tag="IXG20_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1609090.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(31897..32583)
                     /locus_tag="IXG20_000877"
     CDS             complement(31897..32583)
                     /locus_tag="IXG20_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1609091.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(32576..33610)
                     /locus_tag="IXG20_000878"
     CDS             complement(32576..33610)
                     /locus_tag="IXG20_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1609092.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(34005..34343)
                     /locus_tag="IXG20_000879"
     CDS             complement(34005..34343)
                     /locus_tag="IXG20_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ1609093.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(34345..34701)
                     /locus_tag="IXG20_000880"
     CDS             complement(34345..34701)
                     /locus_tag="IXG20_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ1609094.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(34707..35894)
                     /locus_tag="IXG20_000881"
     CDS             complement(34707..35894)
                     /locus_tag="IXG20_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1609095.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(36085..36930)
                     /locus_tag="IXG20_000882"
     CDS             complement(36085..36930)
                     /locus_tag="IXG20_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1609096.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            37164..38129
                     /locus_tag="IXG20_000883"
     CDS             37164..38129
                     /locus_tag="IXG20_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ1609097.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT        11297 a         7838 c         6323 g        12816 t
ORIGIN      
        1 aaaaagattt agatgaacag ttttattgat ttaaatagtt catatttttt atttataccg
       61 cttatttact acttgctgta ctagaatctg aagtgaatgt actcaaaaca ttttcaaagt
      121 ctttgtcttt gatttttaca ttagcgtctt tcaatacttc gccaattact ttattctgga
      181 aggtactgtc acttaatttt gtgtctgttg caatttcttt caattctttt ttgtatttat
      241 ccatgtcgtt gcctttgttt tggtttttca ccattttaac cacataatat tccgtagtat
      301 aagttgaagc attggtagaa gtgatcacat cagaaacttg accatctttc aacttaaagg
      361 ctgcttcttt gacttctgcc ggaaccgttg tagtagtgga atcaaatttc acttttccgc
      421 catcttcttt tgtcgtagta tcagttgact tgtctttggc taatttagag aaatcgtctc
      481 ccttatcagc agctttcttc acatcttttg catcatcttc acttgctact tggataattt
      541 gagcttccac ttctggatgg aaagattccc aagccgtttt taaatcttca tcggtcaatt
      601 tgatatgttt ctttagacct tcttgaaagg ctagactttg tttgatactg tctttgaatg
      661 tttgttctgt gtaacctgca gatttcaatt gtgaatcaaa tgaatcaccc aattgtttct
      721 ttgtttgatc atattgagaa tcgatttctt tggttgatac gtcatctcca tatttatctt
      781 caaatacttg ataaacgatc aactgttgca cagcggaatc tgctccttgc atttttttca
      841 agctatccat tacttcttgt tcgctgattg taccgccttt ataactaacg gctgtttttt
      901 caccggcatt cgtacagcct gacaataagc cagtgcctag tacgatggca agtgctaaaa
      961 atcttttttt catttatcaa ttctcctact tttcgttttt tgcgaattgc taaaaacaaa
     1021 ggtgttcaag atgaaaatca tcaagaacac ctttactaat ctgctcgata cgtcctgaat
     1081 agatataagg ctacacaatc cagaaagttc gctcagcaga ataaatgtca agtagcacat
     1141 cacaccttaa tatactactt cttttgttac cgtttttcaa gtctatcttg cgttccgtat
     1201 ttaacgtaac taattcttag aacaaatgtt atcgattttg tttttcttct tccgctgctg
     1261 ggatattcaa atcagacact tttatgttcg attttccaga catttttttg ttgtttttat
     1321 tatgatatat ttttatacaa ctaaaaatga atatattaaa actaccaaaa ataactttta
     1381 acgctgtttc attatatgtt ttttctattt tatataaact tttggatact taataccaac
     1441 cggttggatt gtaccaacat gagaatgtgt cagttctatt tcctgtcgca cttgcatagg
     1501 cccatactcc ttttgttgca tttgctgatc gaatagagtt tccaactttc gcttttgcac
     1561 cgtgacgatt atagacacga ctatagtggt tagaatgacc tgtttttctc gtacaagttg
     1621 tatcactata ataaacccat cttccagagc tatgattaat tgctgttcta gaaatttccc
     1681 ggtctactgg taaatttgac ttaataatat tacctttagc atcaaaatga atctctcctg
     1741 gctttaaaga tttgtaatag atttcaccgt gaactgaatt tgcttccact tcagatgaca
     1801 tagcgtttcc tccaacaaaa cctatcccta gtgcacaaat aataactgct tttttcataa
     1861 aacgacatcc tttccttttt gatttttaaa gcatgatctt cttgtttcct attttggtca
     1921 gatatggatc atgcgtgact acgataacta acgtaccttg ggaagcttgt tgcgataaga
     1981 tttcaactac tttatcctta tttcttttat ccaatgaccc agtcggttca tcggctaaaa
     2041 tgattttgct attcttcaat aagactctcg cacaggcgat tctttgcttc tcaccaccag
     2101 ataatgtgta gataggcgta cttagcttct tgtttatatt tacttttttc aatgcctcat
     2161 actttgctgt tcgcttatct gaaacattta aattcgaaaa cttcaatcca atatctaaat
     2221 taaaatcgat tgtttcatat tcaatcaacc caaaatcttg aaaaagaaaa gatatggtat
     2281 ttctttttat caagcgcttc tctctcccag ttaattgttt cgtctcttga tcgaacaaat
     2341 agaattgtcc tgaactgaac tgatcattca atcctaatat attcaataac gtacttttac
     2401 ctgctccact ttctccaaca acagtcacaa gatccccagc atccgcagtg aaacgatcca
     2461 aatgaagaat cgttttgttt cgaatgattt ttttcgcacc ttttatcact atcaacggct
     2521 ccaattgtta acctccattc atcagcaata actgactttt cttttctgta gacaaaataa
     2581 aaacaactga aagaataaaa tcaatgaagc taaagaaaaa aatattaaaa agatatttag
     2641 gctctctttc tataagaaaa aggaagatta cacttgtcgt tccccatttg atgaggttca
     2701 tcatgaagaa atctttatac gtttcaaaga gcgaataacc acttgaacga aagatagcga
     2761 ttttccgaca attgtagaca aaatatatgc ataacatgac atagctcaac aagaagaaag
     2821 aaaagaaagt gagtaaaaat atctgtatca agtaatgaaa tgatctgctt aacactttga
     2881 cagctatctc ttcagtctga gtatacggaa atgataactg caagttatca cgtaactgac
     2941 tttcttcaat cagctcaaat aacctttctt tcgacaaaga gcctatcttg atctttaacg
     3001 gtggatagat ttctccgctg agaatatttc tttccaaata catactgttt ttcaatgtca
     3061 gtatttcaac aaccggataa tgttcgatcc aggggataga aggaataaat gtataaatgg
     3121 gttgattgtt ttttataaat agaaatttcg gttctttttc actgatttct tgataatatt
     3181 ctgttatttg cgccaggtca ttttttaaat ctgtatgtct atatcgttca gggatcaata
     3241 taacagggtg cgtttctttt tcggttattt ggatcaacct tccagagaca tcaatcaaag
     3301 gaaatttctt aagataattc agatttattt ttatccctct gccaaactgt tcattttctt
     3361 cattcaagta atctgataca ttgactaata tacttccgtt tttattcaaa gtttcatata
     3421 attcctgatc tgctgctgat gcaaaacgat tggaataaat aaaatccacg tggtttttcc
     3481 cgatggttac tggataaaat acatgatagt cctcaccagc gatttctttt ggtggttcat
     3541 gggaaacagc taactgtgaa gaagaaaaaa cgatagttgc taattcgaca ttggcaacgg
     3601 tcaaaagaag agaaaacaac aatccaaatg taaaaagaat gtattggata tagttgaacg
     3661 cttctcttaa cgaagcgatt ttttcaacta ataaaagaga aatcaaagag ataccatata
     3721 ttccgctgaa caagaaaacg tacatctgga acaaataaag aaattgctcc ttataataca
     3781 taaaaataaa accaccaatg attcctgcaa ttatgaatgt aataatgatt ttctttttcc
     3841 ccaataaaca ccacaatact tgatgtaatg aaaagtcatt attcaacaat aaactgattt
     3901 ctttattcat caataagaaa aaggatgata acaaaaggaa taaaagaatc aatccaatag
     3961 ttaaatacac tcgataatta tatacgtctg tatttgaaat aaccgaatac gaatagctat
     4021 tttcaaagtc ttcaaaaata ttagtggcat aatcagtgtg aaatgcttga ttgaatcgat
     4081 cagtgaatag tgaaatgacc cgttcatagg cttctttttc tctcgtcatc aagaaaaatt
     4141 caccttcaaa atcttttatt ttgatcgatt cttcaatgga cgttacgcga aaggaggtat
     4201 tagaataggg taccttctcg tgagaagatt tgacatcccc ttgataatac aatgtcattt
     4261 caagctttgg ctgtatcttg aagttccaag atgtatctac ttcattggat tcatttactg
     4321 tttttttcat ataagaaagc tcagtttctg atgagacatt ttcaaatacc atgattagct
     4381 gctcatattc ttcttgccca ataaaatgtt gtggcaatcg aatcggagta taagtatctg
     4441 tttgatggaa aaattcattt gtccgataga gtacctgttt atttatacta accaaggcaa
     4501 aaccactcat gataccggtc actatgattg tgagtaaaac tgtgaacttt tttttgatta
     4561 tcaccactct cccttactta aatgattctt tgataaaatc ataccgtttt tcatcaaaaa
     4621 aacgtatcta tcaagtgtat aggtcatgta gaataaatct atatactgta gaatcgaaag
     4681 gagaagaaga tgaacaccgg agaaaaaata cgaacttacc gcagaaattg taagcttacg
     4741 caaaaagaat taggcgaaaa aattggtgtt tccgataaaa cgatttctag ttgggaaaat
     4801 tctcgtacaa tgccagactt tgaaatgctg tcacttttac atcatacttt aggtctgccg
     4861 attgattttc cttctattga atcagctacc gaatcaatag aaccaacatt gccagatgaa
     4921 cctcttgtta acaagaaatc ttattcatca aaacaaccct tctcactcaa aaagacacta
     4981 tctttctttc tgctcgtctt ttgtttggtc tatctttttc ttatcaatta tcaatacaaa
     5041 aatgaaacga tcgatcgatt caatcctttt ttgaaaccac aaatcggcta taccacactc
     5101 cctgtaggat acgtggacaa ttccatcgaa cgttgggtat gtaatgatat ctttggccat
     5161 ggtacatggc tgtcttttca cgctggagag gtagaaaaag agaaaaacca tgctgtaatc
     5221 aaacacaaag gaaaacacgt catgaacgtt cgcctgctca cttgggaagc cattccctca
     5281 gattaccaag aaatcctcag ccaagactat gacgacgcct taaaaagaag ccctcaggag
     5341 ccagataggc attaagtagg agatgaacgt agagatagaa aaaatcaatg cacatgatct
     5401 tatccaaaaa acaggggtac aaattaattg aaaaagcctt gagatgaaag ttcctaaata
     5461 aaaaagttca gaatgatagg tatttctggc ttatagtata atatttacgc cacagcctcc
     5521 caatgattct ttttggaatc ataatcaagg atggacattc acaaaaatat tttgctcatt
     5581 cgatccgaat agattaacga ccaatctagt aagtaatata gattggtacg tagtcaagct
     5641 gcacataatt tagatcaatt ttagttcttt tttatatttc tatgatctgc tcatttactt
     5701 ttatatgatg gcttacagta acataaataa atatgataat cgctggtagt ttatccaaga
     5761 gtactcaata aaatacaaaa tggattgttc aagcgcctat cataatgata aaatgaaaca
     5821 aataaacgat acaaggagga agagactttg agtaatggca gaacaatttt ttcattgtaa
     5881 aaattgttca gttgttaatc tatgggattt cggtagcatg ggcatttttt tatagaggaa
     5941 tcacaccaac ctggccgttt tatctcttgt tagtgggaat attagtagga aattttgccc
     6001 ctcttcacta tcgtacgaac ttttttccaa ctgattctgg cctttttctt aaaacaaaga
     6061 cgcgaaaatt agaaaacatt attgaaatga caattacctg tataatcatt ttcttagtta
     6121 tattcctaaa cgtcttctaa taagaatctc ttaaaattag cttgttacag ttagttacct
     6181 agtttatata acacagttat cttatcttcc ttatcaacat cttttgccaa ctctttgtct
     6241 taaattcgaa acatataagc agagctacga acaccaagac tgtttcggct ctgttttttg
     6301 catcaaattc actcttatat agctgataat ctcggctctt taaaagaaat tattttcgct
     6361 tcattttatt ataaaaaggc taggaacctt gatgttatca ggttcctagc ctttatttaa
     6421 gtattacccc acagaccctt ccatatagat ttttaaatta cacactagtt taaactaaaa
     6481 caaacaagga aaaacttacc atttaatcaa aaaattagct catatgaata tattagaatg
     6541 taaattagac tggtcaaaat ctggtcaaaa aagaggacga atacaggtag tcctcttttt
     6601 tgttattttg atattctaaa tcatttttta ttggattatt cccttgttcg atatagaccc
     6661 aaattgatct atcaataatt atcaaactga gtcatataga gacaaaaatt tcaaaaatga
     6721 cctgttaaat ggtcatcgaa tatcatttaa aatcaatacg acggagtcag acacggagtc
     6781 aaaacaagcc cgctgagagt aaaaaatata cagtcccata ggtaaagtgc cacaggactg
     6841 tatatttttt tatttgagat tacctgaaat gattccattt aatttttatc tcattttttc
     6901 ctactagtta gtatcaaata ctctcctatt ttctcaataa actattttgg gttacatggt
     6961 atctgaaaca gctaaaacct tgtcattata gcaaaaaatc actttatctc cgtttcattt
     7021 cagatacatt tcacttaagc ggattccaaa aaaacaaaag aaaaaggaac ccctagttag
     7081 cctatgcttt ttattggttc ctctcaaaac acggtcatat aacaacgggg tggggtaaat
     7141 gaatgaaaaa ggtggaggaa acattattca atagctgtct taaatacttc gttataaaat
     7201 gtgaacaaat aaaagacatc tatccctatt ttctacactt ccacttgact accatctttg
     7261 aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt cattctcaag tggaatcaac
     7321 tcatcatcta acattttacg ccatgctcgc ttcatttctt gtgggtattt aaatggcttc
     7381 gcttctggat gtttggcttt atatccttcg taatccttac tgttgataat tgctttcata
     7441 ttaggaattg gatcttcacc tggtagatat tgcagattag aagcatgtaa ttctttctca
     7501 gaaacaaacc actcattgga aaagacatga atcaaatcat cactcgcttg tgtaaaatag
     7561 cgacgtttaa tgacaaagat atcttcatta ctatctaaag tccccacatc aaatgcattt
     7621 aaaatttgtc ggtaaacttc tttgactcct tctgttttat tcaatttagc caaagctttt
     7681 tcaatttctt ctcgtgcatc tgaactattc gcagcataag ttcctagtaa ttgatcaata
     7741 taagttgaat caatatcata caacttagtg gtattttcac tataaaattc gatcgttgaa
     7801 aaatcttctc cttctttttc tggttcttcc tctattaatg aacccttcac ggtctcgtac
     7861 acgccaattt gttcttccaa aagttgaatt tgattgttca aagttggaga tttcaccatg
     7921 tcatcattgt aatcgtcata agtcactaac gattcataag cacgattcaa ttcttgaaat
     7981 gctttgacgt actcgatacg gatttcaagt ggcgtatgtt catcaatgga ttccatggat
     8041 gaaacctctt ttaacttacc atatgcttgt ttgaatcttt ttttggattc ctgataactt
     8101 ggataaatca aacctgattc ttcttttgct tttgaataca gttttgtcgc atcctcgaca
     8161 ttcttctcca ttgtcgctgg cttacgaaac gtcacaatca atccttttgt cttatcagga
     8221 tacgtacgat tcgtacgtga aaaagcttgg atcaatcctg catactctag attacgatcc
     8281 acaaataggg tttgaatcgt aggggcgtca aaacctgtta atagacgatc cactacaatc
     8341 gctaaatcga tttgtttccc aaattgtttg aattccgctc tttttcgtgc caaacgatta
     8401 ttgatgtctc cgttataacg ctcaatgtct gctaatgacc aggaagtatc ataatactga
     8461 ttatattcct caataatttt ttgcatttct tcttgttggg cacttgaatc tcggctattt
     8521 tcttctaatg aataggtaat agcgattcgt ggaaaatcag gatcctccat cgttcgtcct
     8581 tcacgaatga gctgattggg aaactctttt gttaaccaat caggctcttt cgtcatctct
     8641 ttgatggccc gataatactt tttggccatg tcaatggaac tcgtggttaa gattgcagat
     8701 ttcgttggcc tgccatttcg aaaatcaaat tttgtatagg cattatctgg acggaacatt
     8761 ttatgaatga ctttttggat atgttcatct gcttcaaaaa cagctgggtc caaataactt
     8821 tcttttttga caggttccat ttgatcaatc atacgattga tctgttctga tgaatactct
     8881 gcatatgttt ctacttcttt taatttccga tagattttat tctctagtga agtcggctcc
     8941 acggtgtttt catgctctac ttgaaatcct aagacagagc catcttctag cgcattctta
     9001 atggtatagg tatgcaagac ctctccatac tgatcatggg ttgtacgtgc caattgccct
     9061 ttggcttgtt tccgattctc aggaaaaatc ggtgtacctg taaagccaaa ccaagtggat
     9121 ttcggaaaca tttttttgat ttcttccata ttttcagcac ttaacgctcg atgacactca
     9181 tctactacaa acacgatatg ttggcccatt aatttttgga agcgattggt tccttttttc
     9241 tcttcttgtt tctttgcata tttcaacgcc gcatctagtt tttgacgggt tgtaatgatt
     9301 acaacatttg cattggcatc tgccaacagg gtttcactta attctttggc acttcccgtt
     9361 ccaacgatca agctattcgc tttggcgttc ccggaagaaa tcccagtatt aaattccgaa
     9421 gcaaattttg taaactctgt ggtggtttga ttatctaaat ctttgcggtc cactaacatg
     9481 atcgttcgat cgatccccga ttttcgtgct aaaagcttag tagatacaaa actagtcaac
     9541 gtctttcctg aaccagtcgc atgccacaca taccctgatt gatgtttatt tgcggcaata
     9601 aacaaggctt gaatcgcatg tacttgataa ggatgtaaca ccatgagggt tttattatct
     9661 tgatcttcac tgacgattgt ataatccgca atcaaacgat gagcatccgg gatatttaac
     9721 acttgtttac aaaactcata taagttttct actttttcat tgtctttcgt tcgccaacta
     9781 aataaaaact tagggtgcaa gtctttcggt aaggcatttg caaaataccg agtggtttgt
     9841 tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat tgttccgaaa cataccttct
     9901 tctgcatatt ttttgatttg gttaaatgcc tgataaacac catcttttgc agttacttgt
     9961 ttcagctcga tttggacaat cggtaatcca ttgattaata aggtgacgtc aaaacgtcga
    10021 tcccgtgctt ctatgttact tccacgtttt gcaatttgat ggaccacttc ataagtagag
    10081 attcctccac caatgtcttg attggaataa aggataagtg agacagaacc caattgcgaa
    10141 tcttcccgtt caatcgtgat ccgagccatc ccattttcac cttttaacca tttcgcagca
    10201 tcaaatgggg ttttcgttcg cagcagtaat tccgttttga tcgtttcaaa ttctttatcg
    10261 gtcaaaggaa aatcattcag ttctgcttga ttatttgaga taattttttg gcgtaaattg
    10321 acccaaaggt cttcttctga ttttagatcc ggacgatacg tccactgatt atgcccttct
    10381 ccaagtacac gaatcaactg ttcttcgact tctgcttcct cacggtgact aattttcatt
    10441 cttcttccct cctagacaaa catcttttgt aagaagcttt ttttcgtttc ttttagtaaa
    10501 tctaacttac gttgatgaag agcgatcgta tcatctagct gtttgaagaa agtaccgatt
    10561 ttttgttgtt catcctttac aggaacctta acactgatat tctcaatatc agaactgtta
    10621 taagaaggca atgcccctac ttgggagtat cttggcaaat ttatagtttc aaacaatatt
    10681 ttaccaaaat agatatccca agtattatta aataaataag ccatggtatt gttatctatt
    10741 aagaaagggc ttgtgaccaa acgttttcta ttaagcatta ttgctgcgcc aactttagca
    10801 aatataatcg ctggcacatc ttttattggc ttccattttt tacgctcaat ttgctcatta
    10861 gatacataat gatttgcatt tctcatttca tactcattac caatgttatt catgtcggaa
    10921 actttaaaaa atggagtacc ttcagaacca ccttgctcgg catcaggaaa tccaattccc
    10981 gattgagttt tacctaattc tcccaactta cgctcttccc aatcttccgt aaaaccagga
    11041 aaccggattt ctggcacttt tgctccgttt tcagggaaca ttttttgtaa gaatcctttt
    11101 ttcgtttcct tcagcaaatc taacttacgt tgatgaagag cgatagtgtc gtcgagtttt
    11161 ttgaagaata tgccgatttt ttgttgttct tttaaatcat tgggaatata gaatactgta
    11221 tctgaaactt ttttccagtc tgaacgaggc atttttgttc cagatgtatc atttgcagcc
    11281 ttttgatatc tatcagcttg aataagagaa taaataaagt ccgagtcgct cttcttagct
    11341 ctaaaaaccc agaaatcccc aatcgctacg cctgtaaaag ttgccttgag ccaatttttt
    11401 aaatatggac gtaactttcc atatagaata tcatttggta aaaattcaat cccttttcta
    11461 ttatcaaatt tctggctcac atctttattc agtctacctt ctcctgccac gatatcttca
    11521 aattcaactt ttggaagtct actggagttt gtacttttat taagtctaac aactaaatca
    11581 attaacttac gctcttccca atcttccgta aatcctggga aacggatttc tggtcgtgta
    11641 tcattgctca tgtttgaaca cctccagcgc accttgtaac cacatttcgt tttcaggtgt
    11701 tgtttgcagg gaagagatag cttctaaaag ttctttttct agcacttgct gttcttgtcg
    11761 tatcttcttg atgtcatttc cgacatgaac catatcaatt ggttcttctt cttcgaaagt
    11821 atctacatag cgtgggatat ttaagttata gtcattttct ttgatttcat caaaggttgc
    11881 caaatgagcg tatttctcga catcttttct ttcagcgtag gtttcgagga ttttttgaat
    11941 gttttcttcc gatagtttat tttgattttt ccctttaaca aattcacgac tagcatcaat
    12001 gaataataca tctcgagttt gacgattctt cttcaacaca atcaccgtcg ttggaatcga
    12061 tgtcccaaag aacaagttcg caggcatacc gatcacagca taaatactgc catcttctag
    12121 taatttttga cggatgaccc cttctgcagc gccacggaat aaaactccgt gtggtaagac
    12181 gatggccatg gtacctgttt ccttcaaatg atagaaccca tgaagaagaa acgcaaaatc
    12241 tgctttggat tttggcgcta atttcccata acgattaaac cgagaatcat ctaagaaagt
    12301 cgtatctgct gaccaattag ccgaataagg tgggttcatt actactgcat caaatgtgta
    12361 aggttcatct gttggccagt ccttattcaa cgtatcccca ttgcggagat tcatttcttc
    12421 ggcgtcgaca ccatgtaaaa tcaagttcat ttttgctaga ttgtatgtcg ttgtatttag
    12481 ttcttgccca tgatatttaa cgttatctgg gtgtgttaag tagttacgta cgtttaacat
    12541 caatgaacct gaacccatcg ttggatcaaa aacactaaag aatcgtcgtt ccttttgatc
    12601 gagcgttacg atttgcgcca tcatatcaga aaccatatga ggtgtgtaaa attctccggc
    12661 tttcttccct gcttctgaag caaattgact gatcagaaac tcatacgcat ccccgatcac
    12721 gtccccatcg tgttctaaaa catctacatc attcaatttc ttgatgactt cggtaatcgt
    12781 cacatttcgt tgttgctcat ctgttcctag tttttttgat tggagatcca catcatcaaa
    12841 taacccatta aattgattgt aggtagatgc caattgcaca aaggctttgt ttagatcgtt
    12901 caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat actctggtgc
    12961 aattgcatat cccaaaatat caacgattgt ggcaatcaga tcttcttttg attcttcatc
    13021 tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga
    13081 ttcatctgct aaaagaacca cttgttccaa caatcgatcg gataaatatt tatagaaaat
    13141 cagtcccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
    13201 cgcactaaat aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt
    13261 taaagttgct atataccctc ttattttagc ataccccctt ttataaaaag caaaaaaaaa
    13321 agaaagaaac ttattcttgc taataaacta cgcgaaagga gtctaacgtt gtttgatata
    13381 acttagactg atcgttaact tattttttat atttctacat agttatcttt agaaccattt
    13441 cagatacata tgaaacaatt acatatgatt ttttcataaa aaaacagtac tgtctagtag
    13501 tataaaatat acaaatttct taaaaatttt ttcactaaaa tcactacctg aaacagtcta
    13561 aaacacttat atatcaacgt ttaatgaaac tatctccgtt tcatttcaga tacaaataaa
    13621 tatagtcggg ctccaaatcc gaaaaaaaat acccccagta gcttatccta agctactgga
    13681 gatgtataag tacggtcata taacaacggg gtagggtaaa taaatgaaaa agatggaaga
    13741 atcattattc aataactttt ttaaatactt cgttgtaatc agttacctgt ccgttcttaa
    13801 ataccacaga ataatctttt tacccatttt caagtaaccg aagtggaatg tctttaccaa
    13861 cttgctctgt cactgcatta ctgatacctg ttagattagc taataaatct gataaagatt
    13921 gagcatctgt agaaatctct gtatcagtat ttatcgtgat tcctttatct tcatttacaa
    13981 gcttccaagt aggtggtata gctgtgtatg tttcgatgaa tgaatcaatt ctttcatcta
    14041 tcgtcttccc agtatctact tctgtctcat tactcttaga tgaatcgatt ctttcatcca
    14101 tcgtctgctc cgtaactgct tctgtctcat tattctcacc ggaaactctc ttcacaccca
    14161 aacttatata ttgtccatct ttagtgtgca tagttaaaat tcccacgctt ttactaataa
    14221 attcactata accagttctt tcaatagaga cttcaatgta tttcacacta tcatactcaa
    14281 aagaaaaata gacatgtaaa agcattttgt tgtttacatc tacataataa tcctttgact
    14341 ctacttgaga ccttcctata cggaatacag ctttactgtc tgatatctct ataccagcat
    14401 ccttattatt aatctgccaa tctccaagta gctcttctgg caagctagta gtttcttgtg
    14461 aacactttac cacttcactg gtagaggtcg aaacattcac ctccgcactg ttagaagaat
    14521 tttccaagga atcaggagcc ttagcacaac caacaagtct ttatatattg tgcagccgat
    14581 attttcttca ttcagtacac ccttgaaata tgcagtgtta gataaagcag ttcgtcctga
    14641 tttgctgagg cacccatctc ttcttgaacg tactccttga ttttcaaggc cgcccgatac
    14701 tctttcggaa aacgctgctt cactgaagcc agcaaaaaat catccttctc cggcaactct
    14761 tccttcttaa tcattcgggc aataaaaaac tgtaaatgcg tcacaaatct taagtaaagc
    14821 atcgaatctt tatcaacctt tttttcggga aacagatatc ctagcatagc caagatatcc
    14881 tttagtgctc ttaccgagag catggtttct tccatatttg cttgttcact ttgggcattt
    14941 acaatgtgaa acgcaatgtt tccagcctcc tcttctggca actgaacatt catttttttg
    15001 ttgatcaatt ggatagcata gcagccgatc tcaaattctt ttgggtaata cttttgaatt
    15061 tcaatcagca ggcgattttg aaaaacaatg cctttttgac tgcgttcaat ggcatgcttc
    15121 aagtgatcaa ccaaggtaaa aaatagactt ttattcagct taaagtcaaa cttggcgttg
    15181 gcataatcga taattcgctt gacgcaaata attagatcat ccggagcagc ctcaaacagt
    15241 ttaatgtaat ccttttttag agcatcagaa agtgaatcta atttaaatac tttttggatt
    15301 ttgtcagccg gaaccaaatc gcctttcttt agttgaaatc caatcccttt ccccatcaaa
    15361 attacttctt tgccttgctt atctaagcaa agaattaaac tgttgtttaa cgttttaatt
    15421 actctcatta gattcaccgt tctctctaag ctaagccatt tcttgcgatt actttttgat
    15481 accaaaagaa gctttctttt tttattcgtt ttaattcttt aagattctca tcggttcggt
    15541 caacatatat aaaaccataa cgcttattga agccttgatg ggtactaaca acatcaatcg
    15601 ctgaccaagg taaataacca aaaacatcta caccatctgt taaagctgct tgaatagcca
    15661 gaatatgttt ggctagataa tcaattcgat cctggtcatt aattcgtcta tcttcctcaa
    15721 ctatgtcggg tgccccatag ccattttcag taattaaaat aggtaaatca tagcgatcgt
    15781 acaatttcct caatgttaat cgtagtccag tagggtcaac tacccagcca tatggcgttt
    15841 tatctacata aggattttca gctgcccggt agacaccttc ttctcccaac atgatctgtt
    15901 gatcccccgc tctagcggaa acatctgatc cgtcattttt gctggcagcg atggtggcag
    15961 ttgaataata attaatcgca ataaaatcgg gtttaccgga ggataaaatt gcctgatcct
    16021 ctgcctcaat tttaggatat aatccacgat cattcaaata gttttcaaac aaataattgt
    16081 attttccccg aactgctaca tctaagaaag accagcctct caatgtttcc caattatggg
    16141 ctgcaattgc atcttctgga ctggaggtag cttgatacat agccgttaaa ttgattgctg
    16201 gaccgatttt tcctttaaga ttcaactgat gatacaagtg aattactcta gcttgagcta
    16261 ataaaacatg gtgattaatt tgaaagagtt ctttttttga aggcagctct ccacttggtg
    16321 gcaaaccaat cgctccagga tgaaggatca tcgtgttttg ctcattaatt gtcagccaat
    16381 agttcacctt gtcaccatac tcagtaagca atacctgtac atagtccaca aaataatcga
    16441 tagttgctcg atttaaccaa ccgccctgct cctctagaaa ataaggtaaa tcgaaatggt
    16501 acatggtgac aatcggttcg attccctgat tcaggcattc atcaattact tgatgataaa
    16561 aagaaattcc cttttgattg gttttattat tttcaatatc cataattcgc gtccaagcga
    16621 tggaaaagcg aaaagctttt aagcctaatt ctttaaacag ctgtatatct tctttatagt
    16681 gatggtaaaa atccgaggca acttcaaaat tagctacccc ttctgtatgc tgatacttgt
    16741 caataataga cggttgtttc ccatcctcct gataggcacc ctctgcctgg taggcactaa
    16801 tggaggcacc ccataaaaac ttctctggaa atgctgaaag ctgtttgtat tgcacaaaaa
    16861 tcctctcctt aactaatttg catcaatggt tcgtgttcct ttaagcttcc gcttgcttca
    16921 ctaattcctg cgtaattttc actgttggtc actaccacaa tcactgtatc atcgtagcca
    16981 gcgtttctaa tggcagcgaa atcggcgttg attaataatt gtcctgctga cacctgatct
    17041 cctgctttta caaatacttc aaagggttcc ccttttaaat tcacggtgtc gatccccaca
    17101 tggatcaata attcaatacc ttctttagaa cgtagaccaa tcgcatgttt agattctaaa
    17161 atagtaatga cttctccctc aacaggtgaa taaatgctgt tttcttctgg atgaatggct
    17221 acgccctttc ccaatagtcc cttagaaaag acagcatcgt tcacttcaga aataggacac
    17281 acggttcctt ttaccggatt ttcaacaatg actgcttcgg ctattgccgt ttgctctgat
    17341 tcatcagaag tatcgtcaaa tccaattaga taagcaatga taaaggttgc aacaaaggcg
    17401 attacacacg taataattgc gtgaacaata ttcattggat tttctccaat aaaggctggt
    17461 aaggctgcta agccagggga aacaaaggca tatctgacta aaccgctcaa gccagcatac
    17521 aaaccagcaa ttgcaccacc aatcatggaa gcaactaatg gttttttcag cttcaaggtt
    17581 actccataga gtgaaggctc agtaattccc attaaagcag tgatcccagt ggacacagct
    17641 agctgcttca actctttgtt tttagttttc gcggctacag caaaggtagc acctgattga
    17701 gcaatattag aagccagcat tcctggccca ttgatcattt cagaacccgt actgctcaat
    17761 tgcatagtag cgattggcgt cattgcccaa gctgtacccg taaccaccaa aaatggctgc
    17821 aaaccaccca tcaaaaatgg aattaaccaa gctgctttgc cgttaatcca ttcagccccc
    17881 attgctataa gatcgttcag caacgttcca aacggtccaa tgacaaccaa cgctaaaggc
    17941 cccataatta aaaataccag catcggctta gtaaagaatt taataaacga aggcgaaatt
    18001 cgatccgcaa agggctctac ataggacatg atccaaacag taaagataat cggtaaaact
    18061 gatcccgcat aatctgcagc taataccgga attcctagaa aagaaacggc atcgccagta
    18121 gaaaaaagct gtgaaatatt tggatgaagc aaaatgccag caacagtaat cgccaaaatt
    18181 gggttgacat caaactttat ggctgcccca taggctaata gtactggcat aaaataaaag
    18241 gctgcatcag caatagtatt caaaatgatg taagtttgac tactgtctga tagtacacca
    18301 gtcaaagtca agacggccaa aatggctttt accatcccgg cacctgttat agctggaatc
    18361 atcggtgtaa aggtggtaga aatgacacta atcaatcttg aaataatact ttcttgattg
    18421 tctgaagagg ggcccttttt aattgaagtt cccacttcct tttgaatttc cttgaaggct
    18481 gtttggactt cattgccaat caccacttgg tattgtccac cgctatccac cacactaatt
    18541 acaccaagtg ttttttctaa ggctttcgta tcggctttcc ttttatcttt gagttcaaaa
    18601 cgcagtctgg tggcacaatg ggttaagctg accacatttt cttttccccc aacctgttgc
    18661 aatacatttc ttcctacttc tctgtaatcc attattctct ctccttaaag cagtaaaaaa
    18721 gaccaagtca aaccacgccc caatcaacat tgaagtgtta gtttgtcttg gtcttgcctg
    18781 cataatcagt aacatgccaa taacgttatt ttgctgtaat ccaataatag cacattatga
    18841 tagcgcaatc aacagggaat ttaatttttt atcttttcgt ttgaccgtat agggtaatat
    18901 aacagttaga tcacatcaac tagggtatat ctgcgtatac ttactgctaa aataagtata
    18961 tctaatatta actataccag taaaaccatt cctacataac cagcgggaaa ttcccaaaat
    19021 cgtgaaagca tcaaaaatgc atataacgga tcacacaact tcaacaattc tgctcttggg
    19081 aaaaattgat gaatttggtg agtaaaggat ggaataaaca gcattccaac aatataggca
    19141 acaatagtga tacttgtttt tttggtaaaa atcagtaagc aatagccaat ttcaatcatt
    19201 ccgagatata aaggtatcaa tagaatgatt tgggctagta ttcttaaaaa ttaactagca
    19261 aatgtttaaa ctgatttatt tctaatctac atgtagtaag tatttcaatt ccaccaatta
    19321 tcaataaaaa taccaaacat atccataata attttttctt tttcatttct tttatattgt
    19381 tataacagtg aataacataa aaattcccga caatcattgt aatatttttt tgtcacctgt
    19441 attagtattt aatttatagg aaactataaa aactccctag tgaatttaca cctttgggag
    19501 ttcctgtttg gacggtatgc ataatacatt taaatcaatt taacattaac acctcagcat
    19561 atggtatctc cacatatata cggtctttct ttggatagta ctcaatattt atgttgatag
    19621 cacttccatt gtctaatata gctttagccc ggtatgtgtt atctccattt tcatgagtaa
    19681 tcgtcacatc atcacatgat cgatttaagt tgtactgttc ttgtaaaatc tgtgttacca
    19741 atggttttga tgacttctct aatccagatg tacctgtcat ttttgaaaaa ataataccta
    19801 taatagccaa gacaacaata cctataatga caaacttctt gttcatattt actttattca
    19861 actttttatc tcctatctcc aaaataattt gttcttatcc ctcttatcag tacattgaca
    19921 tccctaaaag tacaccaaag attgctccag ctacccctgc tatcatcaaa atcgtaccca
    19981 ttttctcatc tttttctcga tataagtagc ccataataac agcaacagca ccaaatacaa
    20041 caggaataaa tgctaacgat actaccatag aaatccaacc aataatcaaa tacagcttcc
    20101 ctgtctcttg tccatgtacg atttgttcct tcatttcatt aattgtctgc tcagtacttt
    20161 tctttactgt gtctgttccg gttgtctcat ctgtttcttg tagtgctcca caaaactggc
    20221 aatacttact ttcatctgtt atttctttgc cacattttct acaatacatg tcttctcctc
    20281 ctaaaatcat tcaattgctt attagttaac tgtttacttt attaaataca tcagaaaaca
    20341 gctcattcat tggtacactt aatatctcag aaagcatctt catttgatag ggtttaggaa
    20401 tcgttctccc tgtttgccac gaacttacac attgctgtgt aacattcatt ttttttgcta
    20461 actccgtttg agaaagtcct ttttcttttc tgtatcggct gagattactt acttttttca
    20521 tgattctccc ctcctgtttt attatataca acaatatgat gtatttcaac tcataaaata
    20581 taaaaaactt caaataccat tacaaataca acaacttgtt gtactatact gagatggggg
    20641 tgtattaatg aaacagaaac aatatgcaaa ggtcattaga gaaaagcgaa aagctctcgg
    20701 gtggacacaa aaagaattag ctgaaaaaat cttctctaca caacaggcag tagcaagatg
    20761 ggaaaattct gtaacagagc ctaacctcga tagcttaaca gctctttcta gagccctagg
    20821 aacccctgta agccattttc tagacaatgt tgtcgtagat tatgaggaag agtttttggc
    20881 tctctatcgt tctcttagca ctgaagatgc agttcgaaca attgactata tgaagctatt
    20941 aaaaaggcaa gaaaatgaac gcaatcagct attgaaagat tgaaaaataa ataacccacc
    21001 gaactttaac ggtgggtgta ttgagatgaa ataagattaa gagctatgga taacctgtct
    21061 agtttaatac aattgggtct tttctcttcc ttactctccg tccatcatct cttgttacac
    21121 ttgtaaattg atacagagga actatcccag ctcctgataa tatcaattct tttattgttt
    21181 catctatccc tgcccctttc gctaaatata ttaaaaaact actacttgct ttcacattgt
    21241 atagtttctt aatatcctta gaagctcgtt caagagtgaa tccttgttta acaaagtagc
    21301 ctctgttttt tattaaagca tttttcacgc tgttatcccc aacattacgt aggcgttcac
    21361 aaacagtgtt aataagttct ttctctgtca ttagatatgt cccttgccac gataagccct
    21421 gtaaaacttc atgtgcttca tctaaaatca attgtttttc cttgctgagt tctgatcggg
    21481 aattttgagg aaatactctt ttagcctctt tttcgttaaa acatatctta acccattcat
    21541 aagaaaaata ttgatatgta aaatgaattt tttccagaag tggaaggata tcttcattca
    21601 atttttgaat cacagaagtt gcattaaaaa cgtctaatcg atagacattg cttctcttat
    21661 gattaaagtt tcctaacgct ttctcttcct ttaacctatt taggtattcg agcatcttca
    21721 aaggaacatc ttcatcatct aattttttta tcaatcctat aaaagtcata agtgatagta
    21781 acttattaca tttatctatg tcataagttt taccatagta agaactcatt tttttagcta
    21841 attgttcccc cgacaaaata caagtcacac tttccttact atcatcaaaa aaattaacat
    21901 cagaaagcaa gacatcatag acacgtcgca tttcctctag ataattcctt attgtcctga
    21961 aaagtttgtc tttcattgat tcaagagtgt caaaaagtac ttttgattcc tctttcaatt
    22021 ttcgaaaatg tgtaggatac aagtctaggt tctctaatag ccaattaaat gtttctaccc
    22081 gactgtcttt ctttaaaatt agcatcacaa ctccaataaa attttcactg atatcacaat
    22141 tactggaatg acaataaaaa atggaatgcc cagttttact ggtaaaaata ctagcagagg
    22201 gattatgatc gttgtgaaac agacatctta agtttttact tgacttaagt aatttattca
    22261 tatcaatggt taacaatctt tcataaatct cattaaaaga catgtcggag gaatcaaaca
    22321 ggcagtcttt aaaccactgt ttcatttctt ctttgtcgtc attttttatc aacttaacaa
    22381 aatcggtttc attgactagt tcatactctc tccctgaatc actttgacaa tttttagaac
    22441 atgtcatggt aaaatcaatc ggaagctcag taatatctaa catgttgtct tcattaatta
    22501 ttatcactgc atctgactta tatcctccaa aaaacattct attcgaagca tttacattgt
    22561 catcacaccc ggggatgttc tttttcaagt attcgtaaac agcaaccatt ttaccatagt
    22621 cagtaattgc ttcttttaat agaaagacaa tcttgtattt ttctagattt tcagagtacc
    22681 gaatactttt ttgtatcgca aacgcagaat tatgaactaa attgatagat aaaacctttt
    22741 ctatgcttaa gtatctacta tcatctttac tcaaatctac taccttcccc ttctcatctt
    22801 taacagtgtt atcgacatcc agaataatta ctctggtttc cacaaaactc tccttgtcat
    22861 tgcctttatt ctttgtcttg cttggaataa atgattgtga tgaatttagt atctctacaa
    22921 actcatctct tgtaacttct ctatagttat ctaaacacct atctcttaca tcagtttttt
    22981 ctttttttgt aactggcttc tctaataatt tttcttgatc tacataaatt ggaatattaa
    23041 tcattgatgt ttccatttgt gtttcctcct tataaaattc tgttaaagac taaaacatac
    23101 agataataaa aacaaactct ttattactat atgttttagt cttatttgtt tactcaacct
    23161 ttataccgtt ccatgaaact atcaatatca gattttttaa atctaatgac cgtatcaatt
    23221 tttataacag gtaacccgta ttgagaaaca tattttctaa tcgttcctac tgatacacct
    23281 agatattcac ttgcctgctg atagtccata tactctagag tgactccaga aataatgtct
    23341 tctagcatag gctttagcac tgtaattatc tctttcactt gttgttcaac ttgtatagaa
    23401 acaatgctat caatgatttg tagagacata tttccccctc ctttcgtgat tgcttgaaca
    23461 aattttctaa gaattatctt cgtatttttt gctcaaaata aaagtatttt gttattaacc
    23521 gattaatcaa acaaaaaatg attaccagtt catcgacata cacataatac ctcgataaaa
    23581 aaggggtgtc aacagctttg taaaatttat ttacaacacc ccttttttga ggtatactaa
    23641 ttttaggagg tgtggtcaat atgataaaac gtccaccaat aaattattta gaacgaaaaa
    23701 agatactcgg aacaaaaata aaagctataa gaaagagtaa aaaattaaca caaccagcat
    23761 ttggtcttat gattaacaac ggtcaattaa ttgacaaaaa aactatctac gaatgggaga
    23821 agggaaccta tttaccaata ccagagcgat tatcccgaat tgctgatttg ggaaatatga
    23881 gtatagagga gttagtttgt ggcaatgtag aagaatatat tttgggaatt attctttata
    23941 gagatagcat tgtactagat ggtataacat ttccagataa aaacctgttc cagcatcttc
    24001 gtcaacagtt cccaccagta cactctaatt tagatacatg gctggataga tattccaaat
    24061 tagaaccaga gatgcaagaa tttattgcta acaagacttg taataaagtt aaaaatgaaa
    24121 aaattagtct tttcaatatc ttaaaaatag aagaattatt catcaatgct attgtagaag
    24181 aatttgacaa taacattctt ttcctcacta gcagtataga agaactacta gaaagaatgg
    24241 tagatgaatg gctacctatt caattgaaag acatgtctta tcccgaagaa gctgtgagag
    24301 aaattactga taatataaac aagctagaac aaacgatttc atcaattggg aaaaaataca
    24361 ctaagaaaaa aatgaaggga ggtgatacca tctagtggta agtactcctt gtaaactaag
    24421 aggagatgat ttaatggcaa aaatatcaaa gtacaaaaag aaaaacggtc aagtggcgtg
    24481 gatgtataaa ggacacattg ccaccgatcc acgaactggt gaaaaaataa atactacgag
    24541 aagaggtttt agtagcaaac aagaagctca aaaagattat gacgagtaca gacatcaaat
    24601 attatacggt gttaaaaaga aagcacctga tatgacattt gaagacctgt ataatgaatg
    24661 gataactcat cagcgtacca gtgttaaagc ttcaacaatc gcaatctcag taagatacgc
    24721 aaataatcaa atacttccag catttggtaa gcttaaacta tcgaatatct ctgtcccata
    24781 ttgtcaaaaa gtggtagatg aatggcacag taaatatgaa agttacgatt atatgagaaa
    24841 acagactgca caaattttac gctacggggt tgcaatgcaa tatattgaca ataatcctat
    24901 ggaaaaaaca cttcttccac gaaaaaaaga gtatgagaaa aatcgtaaat tttactcaaa
    24961 agaggagctt aataatttac tggatgcttt taaagatttt ggtaacatga aacaatacgc
    25021 cttcttcaga ttattggcat acacaggtat gagaaagagt gaagtactcg ctttacagtg
    25081 gaaagacata gacacgttta ataaagaact acatgtcaat aaaactcttg ctgtagatga
    25141 atttggtaaa gttataattc aatctccgaa aacaagagct tctagacgag ttatctcttt
    25201 agatactgag actctttcta tcctcaacaa ctggaaatta cagcaaaaag aagagtattt
    25261 aaaacttgga tacaatacta gctctaaaga gcaacatgtt tttacaacag ttaaaaacac
    25321 tctatacatt ccaaacactg ttaatgattg gttaagatat attttgaaaa aatataactt
    25381 gcctcgtatt acacctcatg ggtttagaca tacacatgct agtttattat tagaagcagg
    25441 agaatctgtc aaggtagttc aacaacgatt agggcatgaa aatagtaaag tgacattaga
    25501 catctatgca catattacaa ataatgcacc taagaaaaca ggtcaagact ttgctgatat
    25561 gatggctcat cagtaaaaga gctggtcaaa atctggtcat ttctagtata caggcaaaac
    25621 aaaaaagcta gaacccttat atatcaaggg ttctagctcc tataacaggt ttacccaaca
    25681 ctgccttcca tctcataact gatcaatcga ttgagttcga ctgcatattc cattggaagt
    25741 tctttggtga atggttcaac aaagcccatg acgatcattt cagttgcttc agattcggtc
    25801 aggcctcggc tcatcagata gtagagttgt tcttctgaga tttttgagac tttggcttcg
    25861 tgttctaaag agacttggct gttgtggatc tcattgaatg ggatcgtatc ggatttcgac
    25921 cattcgtcca tgatgatcgt gtcacattcg atatgagaga tcgaacccgc actgtttttg
    25981 gcaaaggtta cttgtcctcg atagtttact tctccgccgt cttttgaaat tgatttagag
    26041 acgatagagc tagatgtatt tggtgcattg tggatcattt ttgcacccgt atcttggatt
    26101 tggtttgctc ctgcaaaggc gatggagagc atggtgcctc gtgcaccttt tccatctaag
    26161 taaacacttg ggtatttcat cgttgttttt gcacctaggt ttccgtcgat ccattcaacg
    26221 gttgcgcctt cgtatgcttt tgctcgtttg gttactaagt tgtacacatt gtctgaccag
    26281 ttttggatcg ttgtgtaacg gcaatacgca tcttttcttg taaagatttc tacgatggcg
    26341 gcatgcaggc tgttacttga ataagtaggt gccgtacaac cttcgacata gtggatgctt
    26401 gctccttcgt caacaatgat cagcgtacgt tcgaattgcc ccgtgttttc cgcattgatc
    26461 cggaagtacg tttgtaatgg aacatcgact ttgacgcctt ttggcacgta aataaaggtc
    26521 ccacctgacc atacggctga attcagcgct gctaatttgt tgtcggttgg cggtacaagt
    26581 tttgaaaagt actctttgaa taattcaggg tattctttca aagcagagtc agtgtcagta
    26641 aagacgattc ctaacttttc gaattcatct ttcatattat ggtacacgac ttctgattca
    26701 tattgtgcgg aagcaccggc tagataagct cgttcagctt ctggtatacc aatcttttca
    26761 aatgtatctt tgattttttc tggtacgtct tcccagtcac gagcgggttt gtcacttggt
    26821 ttttggtaat atttgatggc atcgaaatcg atgtctgaaa ggtcggggcc ccatgtttgc
    26881 attggcattt tgttaaatgt ttctagagat ttcagacgga attcaagcat ccattctggt
    26941 tcgcctttga ttcttgacat ctcacggatc acttcttctg tcaagccttc tcctgtactg
    27001 aaaaccggtt cgacatcatc gtgaaacccg aatttatatt cttctaattc tggtacgccc
    27061 atgttctcac tcctcttcct tgtggtaatg atggaaagct gcggtaccat cgttttccat
    27121 tgcttttcct agtgctttcc aggcaagtgt cgcacatttg atccgagcgg gaaatttagc
    27181 tacaccacca agcatagcag catcgcctaa ttggtcggga tctttgactt cttttccttg
    27241 gacaagctgt agaaagtcta cagttaaatg ttctgcttct tctattgatt ttccgatgac
    27301 ggcgtctgtc atcatcgagg cacttgccgt actgatggag cagccactgc cgtcaaaggc
    27361 gatatttttg atgattccgt cttttacatc gacttgcaat tcgatcacat ctccacatgt
    27421 ggggttgttc aattcgatct tgcttgtcga gtcagtcaag cttccacgat gatgcggatg
    27481 ggaagaatga tccaagatca cttgacggta taaattgtct aatttagata gtcccatgtt
    27541 ggaaaaactc ctttgttgcc atgattgctt caactaaacg gtcggcgtct tgcgttgtgt
    27601 tgtagaaata aaagctcgcg cgagcagtcg ctgatacttc aaggtacttc aatagcggtt
    27661 gcgcacaatg atgacctgct ctgacggcta ccccttccat gtctaaagct gtcgcgacat
    27721 catggggatg taacccatcc aaattgaatg caacgactcc tgtccgaaaa ttcgggtctt
    27781 gtggaccata aacagtcaat ccatcaattg cttgtaattt tggcaacaca taagcggcta
    27841 gttcttcttc atatcgatgg atattgtcca taccgagttt ttgcaaataa tcgattgcat
    27901 ggcctaatgc gatagcgccg gcgatatttg gtgtccctgc ttcgaatttc caaggtagtt
    27961 ctttccatgt actgtcgtat aattcgacaa aatcgatcat ttcgccacca aattcaacag
    28021 gttccatttc ttctaaccat tttcgtttac catataaaac accaatacct gttggtccgc
    28081 acattttgtg tccgctgaaa gcaaagaaat cacagtctag tgcttggaca tcaatggcca
    28141 tgtgcggggc agattgagca ccatctacta cgatgacccc gccgttttca tggacaagtt
    28201 cagctaattc ttgaacagga ttgaagacac ctaagacatt ggatacatga gcaatggaga
    28261 cgattttggt tttgtccgta atctttttcg ctgcatcctc catatccagg aagccttctt
    28321 ctgttagttc gatatatttc aagacagcac ctgttcgttt tgccaactgc tgccacggga
    28381 taacattaga atgatgttcc atgtaagaga gcactatctc gtctcctgct ttgatatagc
    28441 ggtcgccgaa ggttctggct acccaattca agcttgtcgt cgtaccacga gtaaacagca
    28501 cttctgctgt ttcttttgca tggatgaacg cccgtacttt ttctcttgct tgttcataat
    28561 catgggtcgc gcgttctgct aacgtatgga caccgcgatg aacattggcg ttatcgtgtt
    28621 cgtaatagta cgctaatgcc tggagtactt gttttggctt ttgggtcgtc gctgcattat
    28681 ccagataaac taaaggttca tcattgacgt cttgaaataa gataggaaaa tcattgcgta
    28741 gctttttagc atcgatcatc cattcaactt cccttcgatt acttcgacta attctttccg
    28801 aacttgttct acagggattg ctgttaagac agaaccgagg aatcctcgaa tcactaagcg
    28861 ctctgcttct tctttatgca atcctctact catcagatag tacatttctt ctggatcaac
    28921 tcgtcctaca ctggcagcat gtccagcagt tacttcgttt tcatcgatca aaagaatagg
    28981 attcgcatca ccacgtgctt tatcagaaag catcaagaca cgactttctt gttgggcatc
    29041 tgcacctttt gcccctttca agatgtggcc gataccatta aaggtcaatg ttcctttttc
    29101 tcggatcact ccgtgttgca aaatatgccc gatcgtatga ggggctttat tggttactcg
    29161 tgtatcgatt ccttggattt gtctgcccga gctgattgcc acgattttga cttctgcgtg
    29221 tgcaccttct cctgctaaat ctgagtcaaa gtcagcgatc acatgaccgt cattcatgac
    29281 accaattgcc cagtcgacag aagcatctcg taaaatgtgc ccacggcgat tcatataaga
    29341 agtaatattt tctcctaatt gatctacggc tgaatatttg atttttgaac cagctttagc
    29401 gatgacttcc acgatgatat tacctgatga ttttgctact tgttctttgg ttgtctggaa
    29461 acgttccaga taagaaaatt cactatgttc atctgccacg atcaacacat gcttgaagaa
    29521 gtgctcatcg gatgcgccat cttgaatgaa taaggactcg agtggttcat cgatcacgac
    29581 atttttaggc acatacagga aaataccgct gttcataaaa gctacatgga gtgcagtcaa
    29641 ttggtcttcg ttcatgttta ctgctttttt catataatat tcttggacta gttctggata
    29701 ttcgatcatt gcagtgaaca agtcggtaaa aatgactcct ttttctgcta attccacagg
    29761 cagttgttca aaaatcgtcc atgaaccttg ttggacaagc acaggattgt ctttcattgc
    29821 atcaaatgcc ggaatcgtgc ctgtttcact gatggtttgt gtttctttca catcaaataa
    29881 cgaccagcga tggaatttca cccgatcgat atgcggcaat ggcagttcat ctgctttcgc
    29941 tagtgcgtct tgacggaagg ttctcatcca atcaggttcg cctttactta aagaaaaatc
    30001 atttaccgcg tcaagataat cgagccagtt tttttctttc attcttgctc ccccttacgc
    30061 ttcttcttct ttatagtcga ttcctaattc tttactgata ccagcgtagc cttctgcttc
    30121 cagacgttta gctaggtcag cattccctgt catgactact cggccttcca tcatgatgtg
    30181 aacgacatca ggtgtgatat agttcaataa tcgttgatag tgggtgatga tcaatgcacc
    30241 gaaattgtct ccgcgcattt catttactcc tttagcaacg actttcaaag catcgatatc
    30301 cagacctgaa tcgatttcgt ctaaaattgc aaatgtaggt tctaacatca acaattgcag
    30361 gatctcattc cgtttctttt ctccgccaga aaaaccttcg ttcagataac gttcagccat
    30421 ttcttctggc atatttagta aagccatttt ttcatctaac tttttcaaaa atgccataac
    30481 agaaattttg tcgttttcat cacgtttcgc attgatcgct gcacgcatga attccgcatt
    30541 cgtgatacct ggtatctcac ttggatattg catagctaaa aataagccaa gtcgcgcacg
    30601 ttcatctact tccaagtcta aaacattttt cccgtcaaac aaaatttcgc cttctgttac
    30661 ttcatagttt ggattgccca tgatagcggc agataaggta gattttcctg tgccgttagg
    30721 tcccatgatg gcatggattt ctccggtttt cattgttagg tttacgcctt taaggatttc
    30781 tttttcctcg atcgacacat gtagattttt tatttctaag acagacatgc attatacccc
    30841 caaattctct ttcgttctca ctcattttag actaattgag aatgaagcgc aatgaccttc
    30901 cttactaaaa tcaaatatct tttatttatt attaaacaat tttgatttct aaattagaat
    30961 aggtataaaa aagatgagaa ataagccatt acagacttat ttctcatctt ttttgttcca
    31021 taccttattt tgcatcagcc tttactggtt taacagagcc gccccatttg tctgtgatcc
    31081 attcttggac ttctttgcgg tgaagcactt caatcagttt tttgattttg tcactgtctt
    31141 catcaccttt acgagtcgca ataatattca cgtaaggaga gttgtccttt tcaagcaata
    31201 cggcatcttc taatggattc aaccctgcgc cgtaagcgaa gtttgcgttg attgcaacta
    31261 ggtcaccttc atcattttgg tacgtagaag ctaaaagtga tgggtcgatc gtatggttga
    31321 acttcaagtt cttagggttt tcttcaatat catcgaatgt tgctgtttcc agatctactc
    31381 catctttgac tttgactaag ccagcatctt gtagaattgt gatgatgcgt ccccagtctg
    31441 attcagaatt tgaagtaatg attgttgcac catcttttaa ttcggaaaca tctttgactt
    31501 ttttagagta caagcccatt ggttcgatgt ggacagcacc ggcattgaca aagtcatatc
    31561 ctttttcttt gatttctttg ttcaaataag gaatatgctg gaagtaattg gcatcaatgt
    31621 cgccttcttc caaagattgg ttcggtaata catagtcatc gaatttgacg atttcaagat
    31681 cgattccttc atctttcaat aatggtttca catgttccaa aatctctgcg tgcggtgtcg
    31741 gtgaagcgcc gactttcaat gtcgtgtcgt ctgctttatt tgctgaatct ttgctgtcgc
    31801 tgttgttacc gcatgctgct aatcctacag tcagcaaaag cgctgctgca aatccaaaaa
    31861 ttttcttttt catatcctac atccccctat atcctattat cttttgtctg ttttcttagt
    31921 taagaaatca ccgattcctt gaacgataaa tacgattagt aaaataatta aagtcgcaac
    31981 gaatgtcact gttagattac cacgctggta gccttcctgc caagcaagac ctccaagtcc
    32041 acctgcgccg atcgcgccgg ccatcgcagt aaatccgatc atcgagatcg ttgtaactgt
    32101 taatccagaa atcaatgcgg gaatactttc aggtatcaag accttccaga tgatttccca
    32161 gtaacttgct cccattgcat ctgctgcttc cagtacacca gggctgactt cacgaaaagc
    32221 aatctctact agacgggcat aaaatggtgc tgcggataaa acaagagctg gaatggctgc
    32281 ttgcgcacct agcatagtgc ctacgattgc tttagtaaat ggaatgatca aaaccatcag
    32341 gatcataaat ggcgttgacc ggaaaatatt actgatgatc gatacgattc cataaatgat
    32401 tcgagcatac ggtttgtttt tgcgtccaat agagtaaagg atcaatccca aaactaatcc
    32461 gatgatcgtc actaagacca ttgagattaa tgtcatatat agtgtatcaa ggatagcctg
    32521 attcatcgtt tccgggttga tttgtttaaa atctagatat gtttctgaaa agcttttatc
    32581 cacgatggat cacctcaatt cctactcctt gttctttgat gaacacttct gcttgatcta
    32641 attctttggt ttcaccagtc atcaagacag tcaatgaacc aaaggaacct tctttcactt
    32701 gtttgatctt tccatacaca acgttgacat cgatgttata gcgtcgaatc gcttgggaaa
    32761 tgactggctc gttggcattt ctttcgctaa aggtcaaagt tgccaaacgg ccgcttggat
    32821 tttctttgat gagttcagct aatagttctg tcgaatcagc ttctggttcg atgtcttgtt
    32881 gtacaaagcg tttggttact tcttttttcg gttgtctgaa gacagtcaat acgtctcctt
    32941 cttctacgac tttccccaac tccatcacag cgactttgtt gcagattttg cggatcacat
    33001 tcatttcatg tgtgatcaag acgatggtca aattcaattt cttattgatc gctaacaata
    33061 agtctaatac ttcttctgtt gtttgcgggt ccaatgcgct agtagcctca tcacaaagca
    33121 ggatctcagg atcatttgcc aatgctcgtg cgataccgac gcgttgtttc tgtccgccgg
    33181 aaagttgtga aggatatgct tgtccgcgtc cttctaaccc gactaaacgc aacagttctt
    33241 ctgcccgttc tttacgtttg ctctttggta cgccagctaa ttccagcggc agctggatat
    33301 tttcaagtac agttcttgac cataacaggt tgaaatgctg gaagatcata ccgattttct
    33361 ttcggaatgt acgtaattct ttgtttttta attttgtaat atcttgatcg tttacgatca
    33421 cttcgccgct cgtgggaagc tcaagtccat tcaggagacg gactaatgta ctttttcctg
    33481 cgccagaata gccgacgatt ccataaatat cccctttttc aacgtttagt gaaacgtcat
    33541 tgacggcttg gatgtttccg tttttcccat tgaatgtctt tttgacattc gttagttgga
    33601 ttaatgacat gccattcaca tctttctctt atgatttttc aaacaaaaaa gccttcgccc
    33661 tcttgcctta agcaaaagga cgaaagcttg aacttccgtg ttaccacctt gatttaccgt
    33721 tacttcgcaa taacagcctc ttccagtact tggacttagc ttctttccgt atactgtcgc
    33781 gctgtaacgg gcactcccgt agtaagtttg cttacgctcg cttactctgc tctaagacca
    33841 tcttccatct cattttcgtt acccattttc agcaaacagg gctctcttca aacgtccgtg
    33901 acatgtactc ttcttttcaa agctttctcg tttcatatag gattgcttat gattatagca
    33961 actggaagaa tcagtgtcaa cttttttcaa gtgattttct gattttataa ttcgtcaaat
    34021 tcttttggat caacatcttt gaaacttaag atccacgcat tcatttcatc tgaatcattc
    34081 aagatattga tatcttcacc gattttatca ttaacagaag aaactgtacc cgttagcggg
    34141 ctggcaaatt cattgaccgc tttttccgct tcaacttcga tcagaggttc atcttttttt
    34201 agcgattgtc ctgcttttgg cagattggca aacgtgatgt ctccaagttc ttcttgcgct
    34261 tcatttgtca aaccaacaca gtattctttt ccattaaata agatccaaag gttgtccttt
    34321 ttcttcaaat gcttgcctgc cattttatat cagttctcct tcataaattt tttcattgaa
    34381 tccaattgca acaagctggt cctctcgtaa gatcagtggt cgcttgatca gcataccatc
    34441 tgtcgataag acttggctcg cttttgaagc cgtaaactcg cctacttgat ctttcagtcc
    34501 aagttcccga tatttcatcc cgcttgtgtt gaacaggcga cggattggta cttgactcgt
    34561 ttctagccaa gtctcaatcg ttgaggcctt cggtacttct ttgatcatat caacggtttg
    34621 ataactaacg ccatgctggt ctaaccacgc tttggctttt ttgcatgttg aacatttcgg
    34681 gtaccagtaa aaggtgtaca attttcttac ctccttgaag atcttgcagg tgcttcattt
    34741 tggtatctca tcgataatat caacccgata ccgatcatgt tccctaaaat cgaggatcct
    34801 ccctgactaa tgaaaggcaa tgggatccct gttaaaggaa gtaatccgat atttgctcca
    34861 atattctcaa agacatggaa cagcagcatc atgatcaaac cgcttgcgat ataggcgtaa
    34921 aattcattat tcgtatcgaa acacacccga atcatacgat agattaaaat aaaatacaag
    34981 aaaatgacga atgcaccacc gataaagccg aagttttcac cgataacggt aaaaatcata
    35041 tcggactcac gaacaggcac atagatattg cttacgttga atcctttacc aaacaagcca
    35101 ccggtaccaa ttgccaaaag tccttgggcc ggctgataac tcattcctga cgtatcatgg
    35161 aaagggtcca gccatgaatc aatacgtgca aactgataag atttgaatcc aactttataa
    35221 agcaagtctc ttccgccttc agtcgtaacc aagaagatcg ttcctgctcc aacgactact
    35281 gccaagatta ctacaggtgc gatgatgcgc caagagattc cagacatcag gaaaatcccg
    35341 ccaaagatcg caagaaagac aagcatcgtt ccaaagtcat cttgggccat gatcaatgct
    35401 aaaaccggta ttgtcacgat caagagcttc ccaatcagcc aaaagtccga tttcatcgtc
    35461 cgttctttta cttgcgtatt atgtttagtt acgatcagtg ccatcatcaa gatgtacgca
    35521 atcttcatca actctgctgg ctggaaagta aacgcaccga atcgaaacca gtttttagac
    35581 cctgttgaag cagctaaatt ctgatcatag aatttgagca agagtcccat taccactaac
    35641 cccaaaccat aaaggtaagg agtcagtttc cagatccatt tggaattcaa atgcatgatg
    35701 acaacgattg ctgccgctcc aactaaatac cagacaactt gcatccctac gcctttcaag
    35761 actgctggat tgttcggatc atggctaagt gctacatata atgagagcag accaatcaaa
    35821 cataacagaa agactggtaa aatcacgcca taatcaatac gattatcgtt cgaagctttt
    35881 aatttttttt ccatgtttca tcccttttct taaaaaccat actgctgatt attatagcgg
    35941 tccagttaga taatggaaag tctttccata gacatttggg aaaaattaac tttccgtttg
    36001 tgcagttgct tgagcaaatg cagtacaaaa gcctactgct tatctttagt gtatcataga
    36061 actttttttg atacatgcaa ttatctacca gctgagattc tcctcgctgt gagaagtttc
    36121 tgtgctttca ttgtatcgaa ggatctcttc ggcaatctct agcagctgat cagaaaccag
    36181 attggttcgt tggatgaaat aaaaatatcg atggaaattc aacggagtca ttggcacatt
    36241 ttctggaaca gagcgcttag acaaaatcga gcgaccgatt ccttccttca gcaacgcaac
    36301 aattacatcg ttacttttca cgatcatctt cttatcttgg atattcatct cttcaaaata
    36361 acgctgcgtg taatgaaaaa caccagagtt tgcttctctt acaagccaca atttgctttt
    36421 tgggtcccca gcgatgacta gctggtcttc catcaaagag tatctttgta cgccggaagt
    36481 ctctaatggc ttttcgataa aaccaaaatc ggattcatgt ttcgctactt gttctaatga
    36541 ttcatgagaa ttcgtgattt cgatctcaaa atgcacttta ggaaaccttt tgatcagatg
    36601 tttcatcaac tgaggcaaaa gatagacagc aaaggtgtgg gaagcaacga tccgacatac
    36661 ttcttcttca ttttgttcat tgagcaattt ttgctgagtt tcttcccatt cttctaacaa
    36721 aaccagtgtc cgttcatata ataagtcggc ttgcttagtt ggaagcatct cttttcttcc
    36781 gttacgttga aaaagatcga tattcaattc ccgctccaac tgtttgattt gattcgagac
    36841 tgctggttga gataaaaaca gtacttctgc tgctctggaa aaatttcttg tttcatatac
    36901 tgctcgaaaa gtcgttaata acttaaacat ggcaccactt ccataacttt ttatgataat
    36961 tactataaaa actatttgat ttaaatatac aaaaaaccat ctataataga aatgtacaac
    37021 aaaacagttg ctgatccaac tagtaactta gatgaagaca atcagctgtc tgcttgctcg
    37081 taatcataca ttcctattca ttttacaatg tattcgttca tcttggtact aaaatctatt
    37141 aaataaaaag gagaatatta tatatgaaga actttttgaa aattattccg atcccgatct
    37201 gcgggttgat ccttgggtta gcttcccttg gaaatctgtt taaagactat caccatgttg
    37261 ctttaggcaa tctaactggc ggtatctcta tgattcttat gatattcatt tttgttaaat
    37321 tgtttatctt attcgaacac acgaaacaaa cgttgcatga tccaatcatc gcctccgttt
    37381 caccaacttt tacgatgtca ctcatggtta tttgtactta ttttgtttca tatgaaagta
    37441 ttgcaccaat tgtcaaattc atttggctgt gcgctgttat cttccaagtg atccttgtct
    37501 tttatttcaa ttatcaccat gttatcaaag ctgatctttc aattgaagca atttatccaa
    37561 gctggtttat catttacgtt ggatttgggg tcatcactgt aactgccggg aacttcttcc
    37621 cacttattgg aaaaattttc ttttggattt cattgatctg ctatgcgttc ttattgccga
    37681 tcatcatcaa acgtgtcttt ttcgtaagaa atatggctca tccgacacgt ccgctgatta
    37741 cgatcgttgc agcacctggt tctctttgcc taacaggtta tcttaaaaac tttgctgaac
    37801 ctaattttta tcttgtcgta gcattgttcg ctttatctca agtattgtat ttcgttgtgc
    37861 tgtctatgct tccacgtttg ttcaagcttc gtttttatcc aagctatgca gcctttacat
    37921 tcccgcttgt tatctctgca acagctatat ttactactgt tcattacttc aacagtttag
    37981 gtatctatag tagcgtattg gatttcttaa aagttgcgga atgtctgttt gcaagtgtag
    38041 ttgtcttcta cgttcttgct cattacgtac gtttcttaat taaagaacat agcaaacatg
    38101 gaaaatttgt ttctaaaaaa atcgcttaat aatcgattca ataggttgtg aaaaagctgt
    38161 tctgcatcaa gtaataagaa cggccaaaca aaaacagctg ttcatgtttt tgtactggtt
    38221 gggcttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtc gtaa
//
LOCUS       DACEUF010000018        38110 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.019, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000018 DACEUF010000000
VERSION     DACEUF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.019"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(451..1761)
                     /locus_tag="IXG20_000884"
     CDS             complement(451..1761)
                     /locus_tag="IXG20_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1609098.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IXG20_000885"
     CDS             complement(1773..3191)
                     /locus_tag="IXG20_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1609099.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="IXG20_000886"
     CDS             complement(3431..4999)
                     /locus_tag="IXG20_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ1609100.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="IXG20_000887"
     CDS             complement(5003..5413)
                     /locus_tag="IXG20_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1609101.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="IXG20_000888"
     CDS             complement(5410..6333)
                     /locus_tag="IXG20_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ1609102.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="IXG20_000889"
     CDS             complement(6330..7208)
                     /locus_tag="IXG20_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1609103.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="IXG20_000890"
     CDS             complement(7195..8532)
                     /locus_tag="IXG20_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1609104.1"
                     /translation="MYDILTFGASIAELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="IXG20_000891"
     CDS             complement(8535..9638)
                     /locus_tag="IXG20_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1609105.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="IXG20_000892"
     CDS             complement(9712..11220)
                     /locus_tag="IXG20_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1609106.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="IXG20_000893"
     CDS             complement(11186..11878)
                     /locus_tag="IXG20_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1609107.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="IXG20_000894"
     CDS             complement(11900..12874)
                     /locus_tag="IXG20_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1609108.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="IXG20_000895"
     CDS             13174..14046
                     /locus_tag="IXG20_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1609109.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="IXG20_000896"
     CDS             complement(14504..16702)
                     /locus_tag="IXG20_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1609110.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="IXG20_000897"
     CDS             16934..17926
                     /locus_tag="IXG20_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1609111.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="IXG20_000898"
     CDS             17986..19407
                     /locus_tag="IXG20_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ1609112.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="IXG20_000899"
     CDS             19400..20899
                     /locus_tag="IXG20_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ1609113.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="IXG20_000900"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="IXG20_000900"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1609114.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="IXG20_000901"
     CDS             22491..23174
                     /locus_tag="IXG20_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ1609115.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="IXG20_000902"
     CDS             23176..24012
                     /locus_tag="IXG20_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1609116.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="IXG20_000903"
     CDS             24023..25777
                     /locus_tag="IXG20_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609117.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="IXG20_000904"
     CDS             complement(26053..26394)
                     /locus_tag="IXG20_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1609118.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="IXG20_000905"
     CDS             complement(26462..28633)
                     /locus_tag="IXG20_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1609119.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28844..29701
                     /locus_tag="IXG20_000906"
     CDS             28844..29701
                     /locus_tag="IXG20_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1609120.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="IXG20_000907"
     CDS             complement(29772..30629)
                     /locus_tag="IXG20_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1609121.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="IXG20_000908"
     CDS             complement(30614..33316)
                     /locus_tag="IXG20_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1609122.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="IXG20_000909"
     CDS             complement(33329..34618)
                     /locus_tag="IXG20_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ1609123.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="IXG20_000910"
     CDS             34768..35814
                     /locus_tag="IXG20_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1609124.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="IXG20_000911"
     CDS             35816..37969
                     /locus_tag="IXG20_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1609125.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11245 a         7840 c         6771 g        12254 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcagca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca
//
LOCUS       DACEUF010000019        37588 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.020, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000019 DACEUF010000000
VERSION     DACEUF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.020"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(136..2289)
                     /locus_tag="IXG20_000912"
     CDS             complement(136..2289)
                     /locus_tag="IXG20_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ1609126.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFAPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKALELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYTPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTAAKPLASAKMDLSAKTGTAETVAEGHPDITTVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHMNLTIAKEIMDAYYDMFMAK"
     gene            complement(2583..3431)
                     /locus_tag="IXG20_000913"
     CDS             complement(2583..3431)
                     /locus_tag="IXG20_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1609127.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3655..5595)
                     /gene="thrS"
                     /locus_tag="IXG20_000914"
     CDS             complement(3655..5595)
                     /gene="thrS"
                     /locus_tag="IXG20_000914"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ1609128.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5932..6111)
                     /locus_tag="IXG20_000915"
     CDS             complement(5932..6111)
                     /locus_tag="IXG20_000915"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ1609129.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6158..7981)
                     /gene="cadA"
                     /locus_tag="IXG20_000916"
     CDS             complement(6158..7981)
                     /gene="cadA"
                     /locus_tag="IXG20_000916"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1609130.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8259..10538
                     /locus_tag="IXG20_000917"
     CDS             8259..10538
                     /locus_tag="IXG20_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1609131.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10631..11278)
                     /locus_tag="IXG20_000918"
     CDS             complement(10631..11278)
                     /locus_tag="IXG20_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609132.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11457..12176)
                     /locus_tag="IXG20_000919"
     CDS             complement(11457..12176)
                     /locus_tag="IXG20_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1609133.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12307..13458)
                     /locus_tag="IXG20_000920"
     CDS             complement(12307..13458)
                     /locus_tag="IXG20_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ1609134.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13675..14415)
                     /locus_tag="IXG20_000921"
     CDS             complement(13675..14415)
                     /locus_tag="IXG20_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1609135.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14419..14742)
                     /gene="rsfS"
                     /locus_tag="IXG20_000922"
     CDS             complement(14419..14742)
                     /gene="rsfS"
                     /locus_tag="IXG20_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ1609136.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14799..15392)
                     /locus_tag="IXG20_000923"
     CDS             complement(14799..15392)
                     /locus_tag="IXG20_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1609137.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15376..16023)
                     /locus_tag="IXG20_000924"
     CDS             complement(15376..16023)
                     /locus_tag="IXG20_000924"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ1609138.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16036..16347)
                     /gene="yhbY"
                     /locus_tag="IXG20_000925"
     CDS             complement(16036..16347)
                     /gene="yhbY"
                     /locus_tag="IXG20_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ1609139.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16363..17472)
                     /gene="yqeH"
                     /locus_tag="IXG20_000926"
     CDS             complement(16363..17472)
                     /gene="yqeH"
                     /locus_tag="IXG20_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ1609140.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17469..17996)
                     /locus_tag="IXG20_000927"
     CDS             complement(17469..17996)
                     /locus_tag="IXG20_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ1609141.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18176..18961)
                     /locus_tag="IXG20_000928"
     CDS             complement(18176..18961)
                     /locus_tag="IXG20_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ1609142.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18954..19823)
                     /locus_tag="IXG20_000929"
     CDS             complement(18954..19823)
                     /locus_tag="IXG20_000929"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ1609143.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19824..21203)
                     /locus_tag="IXG20_000930"
     CDS             complement(19824..21203)
                     /locus_tag="IXG20_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ1609144.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21211..21630)
                     /gene="fabZ"
                     /locus_tag="IXG20_000931"
     CDS             complement(21211..21630)
                     /gene="fabZ"
                     /locus_tag="IXG20_000931"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1609145.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21655..22131)
                     /gene="accB"
                     /locus_tag="IXG20_000932"
     CDS             complement(21655..22131)
                     /gene="accB"
                     /locus_tag="IXG20_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ1609146.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22134..23369)
                     /gene="fabF"
                     /locus_tag="IXG20_000933"
     CDS             complement(22134..23369)
                     /gene="fabF"
                     /locus_tag="IXG20_000933"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ1609147.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23388..24125)
                     /gene="fabG"
                     /locus_tag="IXG20_000934"
     CDS             complement(23388..24125)
                     /gene="fabG"
                     /locus_tag="IXG20_000934"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ1609148.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24137..25054)
                     /gene="fabD"
                     /locus_tag="IXG20_000935"
     CDS             complement(24137..25054)
                     /gene="fabD"
                     /locus_tag="IXG20_000935"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1609149.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25161..25385)
                     /locus_tag="IXG20_000936"
     CDS             complement(25161..25385)
                     /locus_tag="IXG20_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1609150.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25438..26400)
                     /locus_tag="IXG20_000937"
     CDS             complement(25438..26400)
                     /locus_tag="IXG20_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ1609151.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26410..26850)
                     /locus_tag="IXG20_000938"
     CDS             complement(26410..26850)
                     /locus_tag="IXG20_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1609152.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27156..28121
                     /locus_tag="IXG20_000939"
     CDS             27156..28121
                     /locus_tag="IXG20_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1609153.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28178..28408)
                     /locus_tag="IXG20_000940"
     CDS             complement(28178..28408)
                     /locus_tag="IXG20_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ1609154.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28494..29381)
                     /locus_tag="IXG20_000941"
     CDS             complement(28494..29381)
                     /locus_tag="IXG20_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ1609155.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29470..30693)
                     /locus_tag="IXG20_000942"
     CDS             complement(29470..30693)
                     /locus_tag="IXG20_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1609156.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30710..31789)
                     /locus_tag="IXG20_000943"
     CDS             complement(30710..31789)
                     /locus_tag="IXG20_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1609157.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(31920..33647)
                     /gene="ptsP"
                     /locus_tag="IXG20_000944"
     CDS             complement(31920..33647)
                     /gene="ptsP"
                     /locus_tag="IXG20_000944"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ1609158.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33647..33913)
                     /locus_tag="IXG20_000945"
     CDS             complement(33647..33913)
                     /locus_tag="IXG20_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ1609159.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34392..36626
                     /locus_tag="IXG20_000946"
     CDS             34392..36626
                     /locus_tag="IXG20_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1609160.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36774..37094
                     /locus_tag="IXG20_000947"
     CDS             36774..37094
                     /locus_tag="IXG20_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1609161.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37299..37523)
                     /locus_tag="IXG20_000948"
     CDS             complement(37299..37523)
                     /locus_tag="IXG20_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ1609162.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT        10546 a         8126 c         6531 g        12385 t
ORIGIN      
        1 cttttttgcc gagtaaacgg tgttcaaaag ccagtttctc ggtatgaagt caaatttaag
       61 caaaaatgtg agaagctatt ttcacaaaat ttttcttcat actcgaaact aagcggcttt
      121 ttcacgacct cttttttatt ttgccatgaa catatcataa tacgcgtcca ttatttcttt
      181 agcaatcgtc aagttcatgt ggtcttgttc gtctaataag ttcggcaaga caacactgat
      241 ggcaatttcc ggattgtctg ttggtccgta tgccacgata ttactattga ctgtcgtaat
      301 atctggatga ccttccgcaa ccgtttctgc agtccctgtt tttgcggaaa ggtccatttt
      361 cgcactggct aatggtttgg ctgctgtgta aggatcggta ccatggacaa cttggtggaa
      421 tccttcacgg aggatcgcca tattttctgc agaaatattg accgtatttt ctactgttgt
      481 gccaatttct ttcttcactt cacccaagtt tccattttcg tcattgccat agatcgcttt
      541 gactaggtgc ggctgtactc gtttcccgcc attagcgact gttgaagcat attgagctaa
      601 ttggattggt gtatatgtgt caaactgtcc aaatgccaaa tcggtaaagt tacctggagt
      661 ataccattct ttgccattat ccgcattgaa cttcttcata taatcaacag aacgtgagat
      721 cccaggagat tcattcggca aatcgatccc agtcgttgta ccaagaccaa actctttgaa
      781 agagtctctc aattgctgat aagctttagc ttcttcatct aaactaggta gtcccattcc
      841 aggtgtatag tctagtccta acatctttaa cgccactttg atcatatacg tattagaaga
      901 caattccaat gctttgactg catcaagagc aacttgtccg cttcgattaa agacagagct
      961 tttttctgaa gcaccttgca gacgaatcgg ctcatcaatc aaaacttgat ttccgctgat
     1021 cgcgttactt gcccagcctg ctgtcaaagt accagccttg actacagacc ctggagcaaa
     1081 ggcgctagta atcgtgccta aagcattctc ggtgatttct ttggaacctt gttcatgact
     1141 gaaaccagtc attgctagca cttctcctgt ctgaggattc atagccactg cataagcacc
     1201 aggcgaatac tgtgctttgc cgttgttgat caatgtttga taattccttt tcaaaatttc
     1261 ttctactttt gattggaatt ctgcattcat agataacatt aaattagaac ctttttcccc
     1321 agagaagatt tctttttgat tcgaaacatt tccttcatta tcaagggaaa cttcatattg
     1381 ggttttggtt ccttgcagga catcttcata ttgtttttcc aaataacttt gtcctacacg
     1441 gtcattccga gaataacctt ttgctaggta tttctctgct tcatctgctg gaagtccttg
     1501 cttttcagtc gtcacactgc caagaatact tttcagtgaa cttgctgcat tatattctcg
     1561 tgtccagtct gtgccagtcg aaactcctgg aagttcagaa gcgcgttctg ccacaacagc
     1621 taactcttga tcagtaacac cagagttttt gatatagaca gtgtttaatt catatgcact
     1681 attcatccgt ttaaagatgg tagcaatttt taattgctcc tcgtttaaat taatctcatc
     1741 agctgtgact ttattgacca tcgcactata ttgttcgctg ttccctaatt gcttttcttt
     1801 tgccgaaagt ctttttgtgg cttctttcaa atgtttggga tcagccagcc aaaaatcttt
     1861 tttatctcta tctgtcagat tttcatctac cggaacatcg atcagcgagt tcagcttcgt
     1921 tgcgatttca agcaaatctt tagccgtaac ttgtgttcct ctcgtatatg tgatggcctg
     1981 attcgcttta ttcgtaacta aagcttttcc tgtggagtcg tagatcattc cgcgaggagt
     2041 actttcttgg accgtaatcg tagaagtaga cttcactcgc gcctccatct cttcgccgtt
     2101 cacgatctgc aaataaccca atctagcaat caacgcaaca aataacgtaa atataatgaa
     2161 aaaaagaaaa tttaagcgaa aaggaacatg ggaccggcga aacgttttgt tcttaggttc
     2221 ttgtgcacct tttcttattt tttctaacca agaagtattt aatatttttt tcataaaatc
     2281 tttcatcatt tcctatcctt ccgcctattt acattcatgc ttcattgtac ccaaaaactt
     2341 cttaataaaa aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa
     2401 tcaacttttt gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa
     2461 aaaataaact tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca
     2521 tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt
     2581 ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg
     2641 ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg
     2701 gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt
     2761 acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt
     2821 tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag
     2881 tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg
     2941 tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct
     3001 tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa
     3061 cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct
     3121 acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata
     3181 tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc
     3241 gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg
     3301 gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg
     3361 tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta
     3421 attacgctca taagttcctc caccctttta atgatagtct atgtaagtat accatatcag
     3481 acccttattc acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata
     3541 tcttatccca tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt
     3601 atctattgtt cggattgctt tctatctact acttttttac aggaaattta cctattagtt
     3661 ttctctgctg taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc
     3721 tacagctgtt tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt
     3781 gtctccaaca actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc
     3841 cattttttca ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt
     3901 gatttcataa gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat
     3961 aggtgccaac catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg
     4021 ttccattgta gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc
     4081 ttctccaaca tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga
     4141 taatgtttct tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa
     4201 ggctgcctcg ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag
     4261 cattgcttgg gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg
     4321 atcgcgatag ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac
     4381 catcaattca agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg
     4441 accatcattc aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc
     4501 atagcgatgc atcattccta attcagcgat acgaattggt aattcacgat aagaatggat
     4561 cgtgtttttg tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc
     4621 gccatctccc atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga
     4681 tgttttgtac aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact
     4741 aacttctttg tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag
     4801 ccagaatggc aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc
     4861 tagtttacga tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc
     4921 tttcttgtca aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc
     4981 acgccaataa gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg
     5041 aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc
     5101 ttcttctggc aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa
     5161 tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat
     5221 catttctgct tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt
     5281 atcgtagtaa aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag
     5341 gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc
     5401 ttcatggtct ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc
     5461 aacgagtttt ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct
     5521 ttcagcaatt gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa
     5581 agtaattttt atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc
     5641 tgaaaaacaa aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg
     5701 ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct
     5761 tgcttcaaca agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc
     5821 ctagcttttc ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt
     5881 atgtgtttat ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt
     5941 ccattcccca tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg
     6001 ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc
     6061 taattgttcc ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc
     6121 tcctctttct gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta
     6181 ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg
     6241 tatccacgac ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa
     6301 ttaacatcaa aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca
     6361 tcgtatctct tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga
     6421 ccgcatctgc actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat
     6481 ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt
     6541 gatctttagg gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta
     6601 cagaatctaa ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga
     6661 tcatcatcaa atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg
     6721 ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga
     6781 ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta
     6841 cttcatgatt attgactact gcttggattc cttgaccggt cacttctttc agttgactaa
     6901 caggatacag ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag
     6961 attcctgttc tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg
     7021 gctgaatcgc ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa
     7081 tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt
     7141 tacttgaacg gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag
     7201 gtgatgccac taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa
     7261 taccaccgat caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga
     7321 caaatcgggc aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc
     7381 tgtctgcggc acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg
     7441 acataatctg atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt
     7501 catcgaccga agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct
     7561 tgactagtaa ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt
     7621 ctttgaatcg atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg
     7681 ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca
     7741 aactagccca gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat
     7801 ctacgccata tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga
     7861 gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt
     7921 cacttaataa agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca
     7981 ttgttttttc tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc
     8041 tttcatctta agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa
     8101 caactatcac tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa
     8161 ggattacttt ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact
     8221 tttaattcgt tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac
     8281 aaacgcacgt ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa
     8341 aacaacaaga attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga
     8401 ttcgagctgg ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc
     8461 aggaattgct tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt
     8521 ctgtcatgaa agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag
     8581 aaacattata tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg
     8641 actggcgcgc accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg
     8701 aaactgaaac agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag
     8761 aaggacgtat tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag
     8821 agatattgga tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag
     8881 cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg
     8941 gaagtggaaa gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca
     9001 agtggctcga ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca
     9061 tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt
     9121 atttggtgca gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt
     9181 ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc
     9241 agattgatcg ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa
     9301 taaatggccg tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg
     9361 aacaattacc tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag
     9421 gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc
     9481 tccaagaact atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat
     9541 tacgtaaaaa actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga
     9601 tcatgaatta tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc
     9661 cactttctct tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa
     9721 ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt
     9781 tactgatgcg ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg
     9841 atgaaatgca ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag
     9901 caaatttgac tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg
     9961 gatcattgga agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct
    10021 atcgctccac aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag
    10081 tagaaacaac agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata
    10141 tcaaattgaa ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc
    10201 gtacagcaat catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag
    10261 acgagttgaa acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca
    10321 ttattttact tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg
    10381 atgtagatgc agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta
    10441 cacgtgcgat gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca
    10501 atatggatat ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat
    10561 taatgttagc agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat
    10621 ttaaggtcat ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa
    10681 gcgaaataat cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc
    10741 tatgacggcc aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt
    10801 ggtcaattga ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac
    10861 gaaatgctag gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc
    10921 tttcgataag aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag
    10981 cctccatccg atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca
    11041 gcggaatcag ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta
    11101 aataacggtt cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga
    11161 atcttgcgta tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta
    11221 ctttgcggta accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat
    11281 tcttccccgt ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc
    11341 ataccacagg ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta
    11401 gtgagattgt cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag
    11461 tcttctaatg ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca
    11521 atcatttttt caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc
    11581 atttggatct cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata
    11641 ctatggaaat ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg
    11701 cgaacatcga tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca
    11761 aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc
    11821 ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc
    11881 aatgtatcaa caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag
    11941 tatgtttcat ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt
    12001 acattataag ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca
    12061 ggatcacctg attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta
    12121 ttattagcat ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt
    12181 tcacttcgct ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt
    12241 gtgaatcacg attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc
    12301 aattatctat actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa
    12361 ttgatagatt tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat
    12421 tcgggaataa atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa
    12481 tccaagtagt cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt
    12541 cagcaagaca taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac
    12601 ctttgacaca aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc
    12661 tattccttcg gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg
    12721 ataacgcaaa tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc
    12781 ttctgtctga ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat
    12841 ggctgttgca ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt
    12901 acgtcggatc ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact
    12961 taatcccaag atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg
    13021 aaaaacctca gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc
    13081 ctcttctatc ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga
    13141 agtctcttct tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac
    13201 tgcaccttta gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac
    13261 taaatccaca ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg
    13321 ttcgcctctt tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat
    13381 ttttcgtgcc atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat
    13441 aatcccgcag cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga
    13501 caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa
    13561 cacaatactt ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat
    13621 ataatttatg ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc
    13681 tttgtatgct acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc
    13741 agaaaatgct tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa
    13801 tggataggta cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg
    13861 ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact
    13921 atcccacaaa aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc
    13981 atctacttga taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg
    14041 atagacttca tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc
    14101 agaaaaacag gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc
    14161 cacaaattgg atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc
    14221 ttctgaaagg tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc
    14281 tccagtaccg catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga
    14341 aaaagctaac cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa
    14401 tgtttcataa gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga
    14461 ccatagtttt tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac
    14521 atctccaaga tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc
    14581 atatttgttt tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc
    14641 gctatttgct gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa
    14701 agcgataatg tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc
    14761 tttactatct atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa
    14821 ttatatgttt cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat
    14881 gtatgttttg tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca
    14941 tcttgtaccc caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg
    15001 tcaaggagac tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct
    15061 gcatcggtga ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt
    15121 gcattattat aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac
    15181 tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca
    15241 ggagaagcac cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc
    15301 ttgaagcgtt gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa
    15361 gcagtatatt cattactata gtccatattc atacaaccct tcattttgaa tataatcaat
    15421 tactttatct ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt
    15481 cgtggaactg atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt
    15541 tgtgtatcct ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt
    15601 ataccatttt ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc
    15661 cggattgttt tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc
    15721 taattcaatc gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt
    15781 gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg
    15841 catcaaaaat acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg
    15901 tgcaaggtga acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa
    15961 gaattgaggc atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt
    16021 catcatggtc actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt
    16081 tgtggaaggt ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat
    16141 ttctttttct aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga
    16201 tactttgatc agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt
    16261 gatcccacct tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa
    16321 acgcttttgt tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg
    16381 tttcaataca tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt
    16441 gatccagcct agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg
    16501 aacaagctcc ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac
    16561 atgtttttga taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt
    16621 tgacacataa gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc
    16681 gcccaagaaa agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa
    16741 cggtgcaatg atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat
    16801 aattcccggt gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa
    16861 ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat
    16921 gatttgattg ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg
    16981 tccatcgcgg tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc
    17041 actagttaat aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca
    17101 ttgtgttaat ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa
    17161 aagtactgga ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc
    17221 aaaaatatcc accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg
    17281 caaaaagtcg tcatccgtca atgagacatc ttggatctca ttataatggc gtaatctgaa
    17341 acaacgctga caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt
    17401 gtatcctaat tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa
    17461 ttcttctgtc attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag
    17521 gccataattc ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata
    17581 atgggtttta caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata
    17641 atctgatcgc ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg
    17701 ttgatccctc ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca
    17761 atcgctcgcg cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca
    17821 tttttcattt ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca
    17881 atcaacgtat tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca
    17941 ggtgtaatct gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg
    18001 tctcctcatc tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact
    18061 gcatatcagc aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt
    18121 acaaaatgac caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat
    18181 attggcggaa cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt
    18241 ccgtcaatct ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact
    18301 gctcgccgtt gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg
    18361 gcgcagtaat tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa
    18421 tcgacgcaaa tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt
    18481 catccgctaa acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga
    18541 tgatcggtac ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc
    18601 cgcgctcttc tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg
    18661 gtctattgaa cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat
    18721 ggggagaacc aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga
    18781 tcactgttac cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg
    18841 caaataaacg atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca
    18901 agcttgtcca tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca
    18961 cgatctatcc atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat
    19021 tccatgcggg gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg
    19081 aaatcttctg gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg
    19141 atcaagcttt ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact
    19201 cctccggtcg tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt
    19261 ttgacagcag cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt
    19321 gcgccacctg aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat
    19381 aatctggtga tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc
    19441 atcactccaa taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca
    19501 tgaagtcctg ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc
    19561 ggatctttgg ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa
    19621 gctagacgct gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct
    19681 tcaccgatgt ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta
    19741 tcaggaacag atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat
    19801 ttctttttct taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc
    19861 ggtaaaaatt cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga
    19921 ctaatcaaat ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt
    19981 aaggctcgct gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt
    20041 gcaatcattg aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact
    20101 cgcagtccca tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca
    20161 aaatggaaag caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact
    20221 acttcttctt gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact
    20281 aaatcgattc ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt
    20341 tccatgaaat aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca
    20401 taattgactg ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt
    20461 gagatcacta cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa
    20521 tctcgctcgc ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata
    20581 tggcgagcag gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct
    20641 gcttcttgct gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga
    20701 atgcctttcc cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct
    20761 gctatttcca atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg
    20821 acatttgctt cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt
    20881 gcacttttag gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta
    20941 ttttcagata aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg
    21001 ctcagtactt cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt
    21061 gcttcatctg ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg
    21121 gtttggatcc ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta
    21181 gctatcaata ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc
    21241 ggctactttt ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac
    21301 tttcaatatc tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt
    21361 tgctttgtcc aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa
    21421 tgcaactgct cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc
    21481 ttctggaaaa tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat
    21541 ccgttctcct tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg
    21601 aatgatttct tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt
    21661 ttgctgaatc ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat
    21721 ttctgtgatc actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat
    21781 aatgcagata acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc
    21841 gggcgctggt tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa
    21901 tactgccgat tctttttctt tcattatttc tggagcagtt gttgcttgtt cagatatgac
    21961 attgactggc tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact
    22021 tgttttattt ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag
    22081 cgaggattga tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc
    22141 tcccagcgtt tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt
    22201 gcataagtaa aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga
    22261 tcttgttttt ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt
    22321 gccaaggctt cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg
    22381 ctgacgtaag tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc
    22441 tggtcattgg ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt
    22501 acaccagctt cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta
    22561 ggagctgtca tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt
    22621 ttggcaccgc gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca
    22681 cccatcacaa aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt
    22741 gattctgaga gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct
    22801 tctgttcctc cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc
    22861 ccaattgaat tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca
    22921 cctacacgca aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt
    22981 ggagaaactc gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga
    23041 agaccaccga tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta
    23101 tctaaaccgc tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata
    23161 tccattcgtt tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca
    23221 cccgctaagg taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc
    23281 gtcccctctt ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact
    23341 ccatagccag taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg
    23401 accaatccgc catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa
    23461 actgctgttt gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc
    23521 gcttgttcct tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc
    23581 gcaatggcat tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat
    23641 cctactacgc cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca
    23701 gatacactag agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg
    23761 tgctgcgtca tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct
    23821 gcatccattc gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg
    23881 atcgatccaa gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag
    23941 atgtcaccgg aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct
    24001 tttggaatct ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag
    24061 gccatcgctt tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca
    24121 tccataacgt tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa
    24181 cacgcagaac tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg
    24241 ggccgacttc gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga
    24301 aacgaacagg ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta
    24361 tttccgttgt tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta
    24421 ggtccttcgc cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga
    24481 catttagagg gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta
    24541 ctgctttttc ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata
    24601 cgatgccgta cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca
    24661 tcactgcaac cattttcccg cttccagctg gggtagcttc tgtcatataa gcgcctcttt
    24721 ttgctactaa cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat
    24781 attctcctaa actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc
    24841 gataatacgc gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac
    24901 gttcattttc tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat
    24961 caaatgtctg ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg
    25021 caccttggcc actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat
    25081 atgtttgatt cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc
    25141 ccccaaccta attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga
    25201 accgttttga ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg
    25261 atgatttgga ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat
    25321 ttgatttctt cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat
    25381 accataataa tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta
    25441 caactccatg agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt
    25501 ctgctgtttt cctaaggtga tctttccact agaaactgct tcatccaata agatagggat
    25561 gcttgcagca gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat
    25621 ctttactttt cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata
    25681 atcaactggt ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa
    25741 atcaaaaata tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata
    25801 gaacggattt ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc
    25861 tgctaatttt tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc
    25921 tccatcgcca aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt
    25981 ttcaccacca atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct
    26041 taacgcataa acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc
    26101 tcccaatttc ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt
    26161 tgctactaaa ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc
    26221 cgccactaaa atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat
    26281 acccgtcctt gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt
    26341 agtaataatc ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat
    26401 taaatatcct cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa
    26461 ggcatgctgt tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa
    26521 atgctgatgg acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg
    26581 gcggtcatct tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc
    26641 agtggtcaag gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt
    26701 cttgtacatc ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg
    26761 agattttttc agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata
    26821 atcatttact gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat
    26881 caaatctttt gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc
    26941 atttttttac tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca
    27001 gcatttataa tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt
    27061 attttctgtg aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt
    27121 atagtctttt tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc
    27181 tccccatgct gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg
    27241 gtaaagcaaa acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt
    27301 caaaaaaagt attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca
    27361 cggaattaga tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata
    27421 accgacaaat gaattatttc agtaattata attatgtaca tattaccaat aagggatttg
    27481 tattaacaga tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc
    27541 cgtctatttt gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt
    27601 tgttagcttt gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa
    27661 aggaactcca agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa
    27721 gacaacaaca tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata
    27781 tcgaattagt tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac
    27841 catcgctcac cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg
    27901 tttgttatgg tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct
    27961 attatgaaaa gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg
    28021 atcctgtaca aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg
    28081 aactgttaaa caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt
    28141 aggcatcttc aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt
    28201 ccatcgctac gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta
    28261 attcaaatgt atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt
    28321 caaaacggcg aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta
    28381 tgattgcgac taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat
    28441 tgtatcaaaa gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat
    28501 tttttttctg gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca
    28561 ttctcaaaac ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc
    28621 aatacaagat ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt
    28681 ttgacaaaaa aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa
    28741 gaccagttag ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg
    28801 tttttttgtg catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc
    28861 tgattagcca tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta
    28921 ccaaattctt tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg
    28981 atcgtcagtg taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca
    29041 tctgcgcctt tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta
    29101 gtcaaatcaa ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag
    29161 tagatttctt ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct
    29221 gctaccgctt ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt
    29281 gcttttgtcc gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca
    29341 gcacttccca tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa
    29401 tcaaaaatcc ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat
    29461 gtttgaattt tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc
    29521 ttcttctttt cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat
    29581 catagcttcg ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg
    29641 atctttagga acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt
    29701 ttcaaaagtt ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc
    29761 cacgacacaa ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga
    29821 agtagaagca ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc
    29881 cacttctcct gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa
    29941 ctcttctaca taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat
    30001 gacctgagga aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa
    30061 agaaagaatc atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc
    30121 ctgctgagtg atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg
    30181 aagcggtgct attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac
    30241 tacacctgtc gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat
    30301 cacttttcct ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat
    30361 cagaggtatt ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt
    30421 atgggtatga ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc
    30481 tctaacaaca actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac
    30541 atctttttcc aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc
    30601 atgtctttct aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg
    30661 aaagtaggta tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga
    30721 cctgatttac tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat
    30781 ttttaagttc ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt
    30841 gtgtcgtact cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt
    30901 ttgtcttccc catatagcag ttcttgctat cttctaacca gccattatac actggaatat
    30961 ctcgaacaag tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct
    31021 cttcataaga agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc
    31081 cacgaatgta accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga
    31141 tgttccgagg aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca
    31201 tcttcttagc aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac
    31261 aaatgattac tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca
    31321 ttggatcagg gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac
    31381 ggataccata gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg
    31441 ctttttggta caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatccga
    31501 taaaggaatt tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt
    31561 ctcttctggc tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga
    31621 tgtcataatc ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt
    31681 tttgatgatc aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat
    31741 ataataatac cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac
    31801 tgtcactcaa acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa
    31861 aaagaacaaa aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt
    31921 tattttacat attcatgaac aagttcaacg acttcttcca tcatatcgca ttctttcaat
    31981 gcacgatcag caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt
    32041 ttaagaatag atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga
    32101 acagctgttt gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt
    32161 gctgcatcaa ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag
    32221 taagaaacgc gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta
    32281 cctacagaga agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga
    32341 atctcgatca tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat
    32401 ttttgttttt cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc
    32461 atagggaaca tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt
    32521 gtacggaaca tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag
    32581 aatggattca tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc
    32641 attgtacgaa caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca
    32701 tattggtcat cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac
    32761 agaccaactg cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg
    32821 tttgcagcta attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt
    32881 tcccattctg ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt
    32941 tcatctgtag gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct
    33001 tctttgactt tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt
    33061 gccatgatag cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa
    33121 ttgcgatcta attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct
    33181 tcgttgatca ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta
    33241 gcaacgtcac gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg
    33301 aacataccga tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg
    33361 tctttaatat tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg
    33421 gcatcaaata cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc
    33481 tgcaattctt cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta
    33541 tcttctactg ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca
    33601 acggctacac cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc
    33661 cttctttctt cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg
    33721 cagtaatagt aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt
    33781 tcaagtttac tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg
    33841 tttgtactaa tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt
    33901 ctttcttttc catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt
    33961 cttttttagt aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca
    34021 ttttttttcg ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt
    34081 ccggattctt tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc
    34141 ttgtcgcttt ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat
    34201 atgaaacgaa ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga
    34261 atggtcaata gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat
    34321 tcttttacct catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac
    34381 gtgaatgatt catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg
    34441 ccactgtcaa tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca
    34501 aaaatcaaca aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca
    34561 gtttggacga tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc
    34621 aaacaccacc aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag
    34681 gcaatacaga aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag
    34741 gtgatatcga tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa
    34801 atcgccgaac caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag
    34861 ttgttgaagg tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca
    34921 aagaagtcat tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat
    34981 ttgaagaacg tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat
    35041 tcatagatga agttcacgaa atcgtcggtg ctggttctgc tggtgatgga aatatggatg
    35101 ctggtaatat tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta
    35161 ccttaaatga gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg
    35221 tacgggtaga tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac
    35281 gttatgaaga ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt
    35341 tatccaatcg ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg
    35401 aaacaggttc taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa
    35461 aactgcaaga agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag
    35521 cagcttatta tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg
    35581 aagaggaaac acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag
    35641 gtattccagt gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg
    35701 acttgaaagc tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc
    35761 gccgaaaccg tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg
    35821 gaccaactgg tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt
    35881 cacaagattc aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta
    35941 aattgattgg ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa
    36001 aagtacggag aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag
    36061 atgttttgca tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa
    36121 gaacggtcag ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag
    36181 tagaagcaaa cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc
    36241 aattaaacaa ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca
    36301 gtgcattatc aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc
    36361 aaatgctggc agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag
    36421 ttgacttagg ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac
    36481 aaatcgaaga tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag
    36541 caaaactgga taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag
    36601 aatcagccga agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg
    36661 agaaatgccg tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag
    36721 aaattataat aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat
    36781 tggtaaatgt aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg
    36841 cccatctagt caaggtctac acagccggaa ataccactat tgtctacact gaagcccctg
    36901 aacataatga aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag
    36961 atgctaaaaa ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca
    37021 aagtaatcga attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta
    37081 cattagccgt gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaagtaaag
    37141 aacacttttg tctcacgctc taaacacgta taaacggcga aagcagaagc aactccttcg
    37201 gaaataagct gaaattcacg aaaattgctt ttcaaatttt tgtgaattcc agcttatttc
    37261 ttggagttaa acgcttctgt ctcagcctct ttttctttct agctttttga tttttctctt
    37321 ttcttttgta cttttctctt ttcattttca gttctacgcg tgtctttatt tttaactttt
    37381 tcctctttat ggatgattga tcgtactttt tgtgcaattt gctcgttttt gtcccagtct
    37441 tttacatcta ataatcgaca taatcgtaac atctcttctt ttttctgttt ttttctttca
    37501 cgtctttgcc attctgtttc catgtgtcac actctccttt ctttaatcat atgtacgcaa
    37561 aataattttt atattttttt gattagac
//
LOCUS       DACEUF010000020        36410 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.021, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000020 DACEUF010000000
VERSION     DACEUF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.021"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            951..1424
                     /locus_tag="IXG20_000949"
     CDS             951..1424
                     /locus_tag="IXG20_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ1609163.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1433..1660
                     /locus_tag="IXG20_000950"
     CDS             1433..1660
                     /locus_tag="IXG20_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1609164.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2296..2667
                     /locus_tag="IXG20_000951"
     CDS             2296..2667
                     /locus_tag="IXG20_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609165.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2923..3165)
                     /locus_tag="IXG20_000952"
     CDS             complement(2923..3165)
                     /locus_tag="IXG20_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1609166.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3197..4099)
                     /locus_tag="IXG20_000953"
     CDS             complement(3197..4099)
                     /locus_tag="IXG20_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1609167.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4112..4300)
                     /locus_tag="IXG20_000954"
     CDS             complement(4112..4300)
                     /locus_tag="IXG20_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1609168.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4314..4877)
                     /gene="amaP"
                     /locus_tag="IXG20_000955"
     CDS             complement(4314..4877)
                     /gene="amaP"
                     /locus_tag="IXG20_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1609169.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4915..5808)
                     /locus_tag="IXG20_000956"
     CDS             complement(4915..5808)
                     /locus_tag="IXG20_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1609170.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5886..6824)
                     /locus_tag="IXG20_000957"
     CDS             complement(5886..6824)
                     /locus_tag="IXG20_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1609171.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6858..7208)
                     /locus_tag="IXG20_000958"
     CDS             complement(6858..7208)
                     /locus_tag="IXG20_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1609172.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7241..8143)
                     /locus_tag="IXG20_000959"
     CDS             complement(7241..8143)
                     /locus_tag="IXG20_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1609173.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8136..8993)
                     /locus_tag="IXG20_000960"
     CDS             complement(8136..8993)
                     /locus_tag="IXG20_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1609174.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9327..10136
                     /locus_tag="IXG20_000961"
     CDS             9327..10136
                     /locus_tag="IXG20_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1609175.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10176..10673
                     /locus_tag="IXG20_000962"
     CDS             10176..10673
                     /locus_tag="IXG20_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1609176.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11058..11131)
                     /locus_tag="IXG20_000963"
     tRNA            complement(11058..11131)
                     /locus_tag="IXG20_000963"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11095..11097),aa:Ile,seq:cat)
     gene            11318..11641
                     /locus_tag="IXG20_000964"
     CDS             11318..11641
                     /locus_tag="IXG20_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609177.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11805..12059
                     /locus_tag="IXG20_000965"
     CDS             11805..12059
                     /locus_tag="IXG20_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609178.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12129..12374
                     /locus_tag="IXG20_000966"
     CDS             12129..12374
                     /locus_tag="IXG20_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1609179.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12450..12815
                     /locus_tag="IXG20_000967"
     CDS             12450..12815
                     /locus_tag="IXG20_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609180.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12876..13439)
                     /locus_tag="IXG20_000968"
     CDS             complement(12876..13439)
                     /locus_tag="IXG20_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609181.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(14007..14207)
                     /locus_tag="IXG20_000969"
     CDS             complement(14007..14207)
                     /locus_tag="IXG20_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1609182.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            complement(14959..15672)
                     /locus_tag="IXG20_000970"
     CDS             complement(14959..15672)
                     /locus_tag="IXG20_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1609183.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(15665..16750)
                     /gene="uxuA"
                     /locus_tag="IXG20_000971"
     CDS             complement(15665..16750)
                     /gene="uxuA"
                     /locus_tag="IXG20_000971"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1609184.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            complement(16767..17210)
                     /locus_tag="IXG20_000972"
     CDS             complement(16767..17210)
                     /locus_tag="IXG20_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1609185.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            complement(17244..17597)
                     /locus_tag="IXG20_000973"
     CDS             complement(17244..17597)
                     /locus_tag="IXG20_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609186.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            complement(17709..18110)
                     /locus_tag="IXG20_000974"
     CDS             complement(17709..18110)
                     /locus_tag="IXG20_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609187.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            complement(18147..18656)
                     /locus_tag="IXG20_000975"
     CDS             complement(18147..18656)
                     /locus_tag="IXG20_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1609188.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            complement(18678..19535)
                     /locus_tag="IXG20_000976"
     CDS             complement(18678..19535)
                     /locus_tag="IXG20_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1609189.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            complement(19553..20386)
                     /locus_tag="IXG20_000977"
     CDS             complement(19553..20386)
                     /locus_tag="IXG20_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1609190.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(20400..21197)
                     /locus_tag="IXG20_000978"
     CDS             complement(20400..21197)
                     /locus_tag="IXG20_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1609191.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(21230..21514)
                     /locus_tag="IXG20_000979"
     CDS             complement(21230..21514)
                     /locus_tag="IXG20_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1609192.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(21511..22512)
                     /locus_tag="IXG20_000980"
     CDS             complement(21511..22512)
                     /locus_tag="IXG20_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1609193.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(22514..23416)
                     /gene="gnd"
                     /locus_tag="IXG20_000981"
     CDS             complement(22514..23416)
                     /gene="gnd"
                     /locus_tag="IXG20_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ1609194.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            23580..24428
                     /locus_tag="IXG20_000982"
     CDS             23580..24428
                     /locus_tag="IXG20_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1609195.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            25074..25280
                     /locus_tag="IXG20_000983"
     CDS             25074..25280
                     /locus_tag="IXG20_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ1609196.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(25482..26483)
                     /locus_tag="IXG20_000984"
     CDS             complement(25482..26483)
                     /locus_tag="IXG20_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1609197.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(26488..28401)
                     /locus_tag="IXG20_000985"
     CDS             complement(26488..28401)
                     /locus_tag="IXG20_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1609198.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(28569..29075)
                     /locus_tag="IXG20_000986"
     CDS             complement(28569..29075)
                     /locus_tag="IXG20_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1609199.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            29235..29675
                     /locus_tag="IXG20_000987"
     CDS             29235..29675
                     /locus_tag="IXG20_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1609200.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(29701..30858)
                     /locus_tag="IXG20_000988"
     CDS             complement(29701..30858)
                     /locus_tag="IXG20_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609201.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(30861..31217)
                     /locus_tag="IXG20_000989"
     CDS             complement(30861..31217)
                     /locus_tag="IXG20_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609202.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(31515..32489)
                     /gene="sgrA"
                     /locus_tag="IXG20_000990"
     CDS             complement(31515..32489)
                     /gene="sgrA"
                     /locus_tag="IXG20_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1609203.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(32685..33524)
                     /locus_tag="IXG20_000991"
     CDS             complement(32685..33524)
                     /locus_tag="IXG20_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1609204.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            33709..34596
                     /locus_tag="IXG20_000992"
     CDS             33709..34596
                     /locus_tag="IXG20_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ1609205.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(35190..35885)
                     /locus_tag="IXG20_000993"
     CDS             complement(35190..35885)
                     /locus_tag="IXG20_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ1609206.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(35869..36267)
                     /locus_tag="IXG20_000994"
     CDS             complement(35869..36267)
                     /locus_tag="IXG20_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1609207.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT        10714 a         7218 c         5755 g        12723 t
ORIGIN      
        1 ttttttcgga gaattaaaaa atacaataaa taactttagt tactttaatg cgttcttact
       61 ttatttttat attatcattt ttcttattca cttctttggt ttatactatt cttataaata
      121 aaaatgaggt atgtcctatg atgaaaagat tgtactattc attgatcata acgattggct
      181 atttgattgt atcaaatcta ggaaatatgg tatttggtat cagtaaagaa ttcagttgga
      241 ctactacatt atgggaatct ctattctttt tcatcttcgt atttttgtta caaaattatc
      301 gaaaaaaata aacatgtgcc atctatctgt tggcacatgt tttaggattt atggtacttt
      361 ttattcggca atccatttta tttttctact tcttcttaat acctctaaca cttttgattt
      421 ataaactaat ccaatgcatc tacttgcgct tattaagcta tttcagctag atagtaccac
      481 cccatgcgtc aatgcttgat tttccttttg tataacattg tataagcaat aaaggacaaa
      541 taagtggtga ttacaccaaa ccattgaata agaaaaattc tgatacgtaa ataaacatga
      601 acttgaaaaa attaatgaat cggttagcta ctacgtagtt gtcttttttc tcatacagtt
      661 ctgttaactt acacttctct tcgctagata ataaaaaatc ctccaactgt attgtccaat
      721 ataatcccag gcatctaaac tagcattata gaagagacac acatagaatc taaaataact
      781 ggttttaaat gtttcttaca atcaaaaaaa gctgttaaat gcactccaca ccttcatact
      841 ctatcatttt ttataaacga tatattttta ttgtttttcg ataaaaaata gttgttttac
      901 aataattatt ctgctatatt ttagctatca aagataaaag agggataaaa atgaaattta
      961 aaacattgct attaaaattt gtcgttttag gtataagtgc cgtatttatt ttgttcggcg
     1021 tactttttta tactcagatc gctacaagcg aaaaaggatt caattttgat tggcagacca
     1081 cattatttgt ttctgtcatt tactttgccc ttctcctagg gatcgttatt gcttatttct
     1141 tattgaaact attgaaaaaa atcgaagata accaagcttt ttccactcaa agcttaaaca
     1201 tactaaaaaa aatcgaacga accattttgt ttattggttt cgcctttttt ggcttacttc
     1261 ctaaatttat gaaagtagcg gatttagacg atgctccagg tttgatgttg gtaggaatcg
     1321 ctgtcgtatt cattcctttt gcagtataca cattaacaag cgtgctggaa aaactgcttg
     1381 aacatgcgat tttacttaag acagatcatg atttgacagt gtgaggtgcg acatggcaat
     1441 tatcgttaat ttagatgtag tattagctca aaaaaaaatg agtgtgactg aattgagtga
     1501 gaaagtgggc attacgatgg caaacatctc tattctaaaa aatggaaaag caaaagccat
     1561 ccgtttttcc actctcgaaa aaatctgcca agttttggag tgtcaaccag gtgatttgtt
     1621 gacctataaa aatgactcga atactatacc agaagaataa aataaagtag aacaaacagt
     1681 aaataaaaaa taatctgaat gatagatgtg tgagactttt agaagttatt tacgtctatt
     1741 attcagattt tttatttcga ttgaataccg ttttatctat aaataagata tgtactgact
     1801 catacaaagg gggccttttc ttctccatgt actatcactt ttcctgatac aactggtttc
     1861 ctgttacgtt gtagttggat ctgcttttag tataatactt tattcattcc accagactga
     1921 tagcagtttg agcagtctaa ataatcatgt agtattacag gccattctgt ttcatacctt
     1981 gaagtgctta aaacttcagc taatggccca ttgtgttaac tcttactcca ttctttttta
     2041 gtaagctccc atatatactg taaagccttc tatatattat gtcatgttgc tttttaacat
     2101 ttactatgta gttttgataa aatgatttga ataacagtga tggacaattc ttttttattt
     2161 ttaacattat tttgataata atgattattt atttaaaaat acattagata acatttgtta
     2221 cgacacgaaa aagcttcaca aacaaatatc gttattttaa aatgagggcg aactcacaca
     2281 attaggagga atagtatgcc atttattgtt tacttttttc tttcattact cgtgatctat
     2341 attccattac caacattttt cctgctcgtc aatcgtctag cagagcaaca tgatacgatt
     2401 gctgctctta cgaaactttg tttacttcta tttgtttgtc tagattactt tacgagctat
     2461 tattggctaa aacagacaag agtaaaaaag agatattatt tcttccttgt ctttttcaat
     2521 ctctttgagt ttctgatcca ttgcttttta cttcatcagt atcatacgat ccatttgtgg
     2581 atcatctggg ttttcaaagt ggtcgtatta ttttgtatgt tcacctttcc tatctcaaga
     2641 aaatttaggg aggcattgtt ttattaaaga agcttctctg tatcaataaa gacacattta
     2701 aggaaaaact tttaaaaatc taattgggaa tttgcccgca taaataagag gctgtgacaa
     2761 aagtcttgtc acagcctctt attccgtata aacagtgttc aaaagcttgc atcttcgata
     2821 atatgcccaa ccaaatgaaa aatatgagaa gctatttttg tttggctgtt cttattactt
     2881 gatgaagaac agctttttca cgacctcttt ttaaaagtct ttttacagtt ttcgagcaaa
     2941 gaatgatacg attagtacta aaatgatggc accaatcaat gatggcacga ttgccattcc
     3001 tgcaatactt ggtccccaag aacctaataa tgcttgacca agtgctgatc caactaaacc
     3061 tgctacgaca tttgcaataa tccccatcga agaaccttta ttcgtgattg ctcctgcaat
     3121 tgctccgata attcctccga cgataagtga ccaaataaat cccatgtttc gttcctccta
     3181 atgttttact tccttattac tgaacccgtg gttctgtatt ttcttttact ttttgtgcgc
     3241 cgcttccagc tgctgatttt actttatctg tttgattcga agcaaattca ccggtttttt
     3301 tcgctgcgtc agttactttg tcttgcaccg tttcttgatc tttttcaaat tcttcttttg
     3361 ttttgatgtc cactacgtta gcgttgactt cgatcacttc taaatgtgtc atatttttta
     3421 cttctttgcc gattacttct tgcatttctt caaagatttt ttcgatatct ttgccatatt
     3481 ccacaatgac atccatatca actgctactt gttttttacc gacttctgtt tcgatcccag
     3541 ccgttacatt atctgtattc acaagttttc ctgccacatt agagaagaaa ccaccgtttg
     3601 cagataataa accgtctact tgttcaattg cgattccgat gattttttgc acaactttat
     3661 cttcataatt cagctcaccg cgcattgaat cttcctgttc agaatcatgc tttccttggt
     3721 tatttgtatg atcagaacca agttttcctt gtccgtttgt gtggtcagaa ctaagttttt
     3781 cttgtgcttt cgcatggtca gaaggatgtt tttgctcttc cacaggttta gaaggatcga
     3841 ttgcatcatg ttgttctgga actttagatt ctggagttcc tggatgatga ggtgcatgct
     3901 tttctgcatc atgtttcttc tcttctactc cttgttgaac gttagggact actggatctg
     3961 gatgtgcaga tactggtgct gttttgttct tttcatcgtt cacaccattt gattggtttg
     4021 gtgtcgttgg gtctggatgt gcagacactg gtttttggtt gttcatttta ttgttattat
     4081 ttttgttatt gttattcatg gatgtttcct cctaagattt aaaatagtga tctaagattt
     4141 tcgtttgttt cagataaaaa ccgatagcga cgccgatcgc tgccatgatc aagatcaata
     4201 aggtcttgaa aaagcccacg gtgatccata aaacggctaa tatcaatcca agtaccccaa
     4261 agatgatagg gagcttgtat gtcgaaaata gttctttcat ttttggtccc cccctattct
     4321 actcgtttgc ggtcattgct tcgtttagct ggttcttcaa agcctgtgta tttcaccgat
     4381 actttcactg gctcttttgt tcccaatatg tgttgtactt cattttcgat ttctttcatc
     4441 aatgttcctg ttttaccaat gagtgctgaa gttcgtttca actccccttt tacattgacg
     4501 gcgatcttgt ttttcgttgc tttgacattt acttttggtg aatggatgaa ttcatcttca
     4561 tgcaagtgag aacgaaccat tccttcaatt gcttttttat ccaacgtcaa ctccccatca
     4621 ccatgtttca gtagaaagtt tccgattgtt tttggataaa acaggaaaac aagtaacgtg
     4681 ataattgtca aaatagcaaa tgcaacggca agccaaaata catattgttg cacgtatgaa
     4741 ttagtgatgg aaaagaatcg aaacttctct agctgaaaag gcaatggata ggttgcactt
     4801 aatagtatcg caaacaaaag cacagatagc aaaagtaatg ttccgatcac tccgatcgtt
     4861 tttacacctt tattcatttt cgcacctcct cataggggtg aaacgatcaa cttcttataa
     4921 gttgatcgct tctccaccag ttactccgta aatttgggct gtaacataac ttgctttatt
     4981 cgatgctaag aaaacataaa ctggtgctaa ttctcctggc tgacctgcac gttctagtaa
     5041 actttgctga ccaaattcag ggatcgaatc agaaggttgt ccttcgtcta gttgtaatgg
     5101 tgtccagatt ggaccaggtg ctacaccatt tacacggatt cctttaggtg caagctgttt
     5161 tgctaaaccg atcgtaaagt tagcgattga agcttttgtt gctgcgtaat ctaataaatg
     5221 ttcgcttgga ttgaatgctt gaacagaagt tgttgttacg attgctcctc ctgctgggat
     5281 atgcggaaca gcttctttca ctaaagcaaa catagcgatg atgtttatca taaatgtgtc
     5341 tttgacttgc tcaagtggta actcagcaat agactgacaa gaaatttgct gtcctgcgtt
     5401 tagtaccatc gtatctagcc cgccaagttc actcacggct ttatctatga tttctttagg
     5461 tgcttgttcg tcacgaagat caaaaggtag taagacaatc ttacgtcctg catctttcac
     5521 gtattttgct acttcctctg catctttttc ttctcctggg aagaattgaa gagcgatatc
     5581 tgccccttct ctagcaaatg caatcgctgc tgcacgaccg atccctgaat ctccaccagt
     5641 gattagtaca cggcggtttt ctagttgatt attgccttca taggattctt ctccacaatc
     5701 aggaacagga atcatattct gctgtaaagc tggtgtatcc tgatcttgtt taggaaattt
     5761 ctccgtatgg tacaagtttc gcgggtcgat taattttggt tctgtcattg ctgtattcct
     5821 cctagttttt aaattgtatt gccaaaacaa atttctttgt ttgatacctg ttttaaatag
     5881 attctttaat ttgtataatc tttccggtat aaatggtaac ctaaaactac tgctgctatc
     5941 aaagagccaa agactacgag tataaagcct agagcagaac ttttacccgg taatcctcct
     6001 gtattcattc cccaaatccc tgcgaacaac gccggaacgg aaatgattaa tgcagctgaa
     6061 tctagatatt tcatgatatg atttagatta ttgtccatca tcccgttgaa tagatcacca
     6121 atactctcta ataaatctcg atagatctcg atcatttttt ctgtttgtcg ctgtctcaaa
     6181 tggacatcat agagtagctg ttcatcgtct aaacgtttga tgaaagtttc agaattccat
     6241 aattgatcca ttgtttcccg ttgatctctt aaggtatgat cgatatatac gatgtctctt
     6301 tcaagatctg cctgtttata cagttcttca ttttccgttg ttttacgagc tgctttatcc
     6361 aaagcgtcaa ttcgcttttt catctctctt agttctttca cgtaatgggt gtaaatatct
     6421 aataatgtat acgtgatgat cttttcaaac gagtttaaat gatttccatg ctttgtcagc
     6481 agccgatcca caaattcggt ttttctatcg atacaagtaa tcaagagatt atcggaaagt
     6541 acaaaagata ttggcgttaa ttttttttcg atacgattat ctgactgatc tagattaatc
     6601 actaccaaag aaaatggatg ctctagtttc gtctcataaa ggcgctccat ccgagaaact
     6661 tcttccggat attcggttcc tacgaaaata tcatcaggaa gctcgaattc ttcgattgct
     6721 tgctcaatct cttcgggctt tgcactatca agtacgagcc aattatattt tttttcatct
     6781 gtttctatta cattcttttc ttcatccaca aagtagtatc tcaatgtata cctccttttt
     6841 ggactttctc gaaactatta gctgaagaag cagttcttta atttgatatt ataatcgagt
     6901 actgcgattt cattttctaa tgaaaagctg tcaagctgtt tgttgaagtc catgttcaat
     6961 cgtttaccga aataattttg ctctaattta gatgtataga tgtccaataa tggatgctcg
     7021 attttagtcg catattctcc aaattccgaa acaaaaggaa tgatttgaaa aaagctgcct
     7081 tttgcaaaag cacctttgtc ccttagaaat ggatcggaat cattgataag cgcgagtgcg
     7141 atttttgtat tttccccaac cttttcaatt aacgtctcta ctgcattttt agacattgta
     7201 atttccattc ctactccccc tcttcagttg ttgtcttcta ttatttaaca actacatctg
     7261 tttttggttg aacttttgca tattttggat atttagggaa tttagctaca caacggaaag
     7321 ccgtcatccc tgcatatttt tcgtaatatt tcttcgttgg cccttcaaaa tccttgctcc
     7381 acggtttcac atgtcctgaa aaatggataa tgtaaggatt gttgcgagtt tcttcgtatt
     7441 cacgttcacc tgctgctgtt ggatgttttt ttgcttttgc catgatatag ccttgtgcat
     7501 tccaacgcgg atgaagtggt aaccaacggt catgtaagat agcatttaat gcatcttggt
     7561 catggaatcg taatttttct ggattatgtt cgatgaaatc taatactttt tgcgtaatat
     7621 tttcatctaa ccattttttc acattgatca gcatcaaacc tgagttgaaa taacgcattg
     7681 atttagctga aattttcatt ttttccagtc gttgatggaa cccagcgtct tcaacagctg
     7741 ctacgattga atcaccgaag tcaagacgcc ataatttaga gatatcttgt aaagcgatca
     7801 tgtcgcaatc catatataag atacgttcaa cttctgtgcc gcggaacaat tccggaatcg
     7861 caatacgata ataagctgtt tctggaatac gatcgctgat taccacattt gtaaagaatt
     7921 ctttattgat tttcaagaat tccacatctg agttcattcg tgcgtttttc actgagaagc
     7981 gtaaaagctg tttactttca aaatcgatat catcgtcaat cacatagaac cgaacaaatt
     8041 tagacgggtt gcaattatct aatactgaga caaataatgc agctaaatgt ggtacgaatt
     8101 ttgtgttaca acttgcgact actgctattt cttttttatt cattgatctc gcctactttc
     8161 gttagagtac tgtgtgctaa tttttttaag tataaattgg tatacggatg atctttcaac
     8221 gtattccaag gtttatcatg tcctgtaaag tgaacgatcg aaggtttttc atttccttct
     8281 ttatactgac gttcatattt ttcatttggt gctggatgac gttcaaaaat cagcgaagtc
     8341 tgcatgttcc attttggatg aagttgttcc caatcttcat aaagtacagc attcaaagca
     8401 tcttggtcgt gatagatgat tcgatcgcca ttttcactga ggtaatggat agttttttct
     8461 gtaatttctt ttttattcca ttgatcaatg tcaatcacca tcactcctga attgaagtac
     8521 aagtcatcgg aatcgattcc taatctttcc aaagctttca cttggcctgg atcgataata
     8581 gcgccgattg tttttccatt taacggctca tcatatagtt ttacgatatc gtctagtact
     8641 aacacatctg aatctaagta aaggactttc ttataatcct ctttagctaa taagtttggt
     8701 aaagaaattc ttaagtaagc ggtagttgtg atatggtcac tgactaggaa gtcttcgtag
     8761 atatcttttt ccactgtcag gaattggata cttgcattac ttgaatattt atttaccgtt
     8821 tcttccaatc cttctttact atattctgat agaccatcgt caatcacata aaattttatg
     8881 cgtcttgttt tattgcaatt ttctaatgca gttgcgatca tcacgctcaa gtaaggagca
     8941 taattttcgt ctgaagcagt taccacagga actacgccgt atctcttctc catcaaaatc
     9001 actccttttt ctaatctttt tctaacctgt tctacatcta gaatattcct catttggttt
     9061 taaagcaaaa tatatgcttt gttttcattg aatattttaa aattttttta tcacaaaacg
     9121 catcggtaag cgctaactat tcgtttttca tggcttttta ttcaaaggta ttgaccaaaa
     9181 actactatat atagccacct ttttcttaag aatgtttttt gaaaaaattc ataatgacaa
     9241 agcaagttag tttcattttt ttaaattctc atttaatctt aaggtaacag gaaaatagat
     9301 atgtaaaaag aaagtaggaa gataaaatga aactagcagc gattgattta gacggtacct
     9361 tgcttgatcg aaatggaaag ataccagaag aaaatatcca agcacttcgg aaatttgatg
     9421 aaaatggcgg aatcgtaacc gtcgctaccg gaaggaacag catttctgct aaagatattt
     9481 tttccgaatt aggaatcggt ggctatttga tttcttccaa tggttcactg attgctgaaa
     9541 tgaaggatgg aaaaatggcg aaagttctca gacgttcaaa aatagatagc gatacactga
     9601 gaaaagcctt cttctatgct caagaagcga aaatatccat cattgctagc cgagaaaccc
     9661 aagatgatca gctgacattc gatgaatcaa atctagtcaa agatgaccct tattatgaac
     9721 atttcaattt acagaaccat tcttttgatc aaattttaaa tatgttggaa gatccttctc
     9781 ttagctatct taaattagca ttaacagata agagtgaaga aaaactacag aagctacaaa
     9841 gagatctgca agcagatggt atccaatcag tattctctga tccgcatttc ttagaaatca
     9901 cgcctacagg tatcactaaa gctcattcac ttgtttttct ggcggattac cttggaatcg
     9961 aatcgagtga catcatggct tttggcgatc aagaaaacga cattgctatg ttgaattaca
    10021 gcgggttgag tattgcgatg gggaatgcac agcgacaagt aaaagaaatt gcaaaccaag
    10081 taacactgac gaatgaagaa gctggtgttg cttatatttt aaaaaacttc tattaaaaag
    10141 aataaaatga cgatatgaat gtaggaggaa agaaaatgtt agttattatt gatatgcaaa
    10201 atcatattct tgatccaaac agcgaatttt atattccaga gtcagatcaa ttggcagaac
    10261 gtattgctca aagattagct aaagcacgcc aaaataatga atatattctc ttcacacggg
    10321 atataccagt tgaactgaag gacaaagaag aagaacgaga agatttgcaa ttgatccctc
    10381 ttctttctcc acaagaaaat gaacgagtga ttaaaaagta ttactttacg attccacctg
    10441 aaactttagc tgaaatccag gaaactttat ttgaaagcaa agaagaacaa aaagagatcg
    10501 aagtagtcgg gattgaaaca aacttgtgtg tactctctaa tctgataggc ctgcagagtg
    10561 cttttcctga agcagacttt tttgttgatc caatgttagt ctccagtaga aaacacggag
    10621 aatccgcttt agagctgttg aaagatttca acgtctctat cgtcgaatca taaaaatatc
    10681 tatcttcatt tacgatattt aacacacaat aaaaaaagca cgcagaactg ttttagtcct
    10741 acgtgctttt tctatcaaag tagattggct tttttatgac acagagtgaa acggcaggct
    10801 cacgacctct ttcttaaatg gtgttcaatc agctgaccct cggtattagc ttaacaaacg
    10861 gcaaaatatg aggaactatt tttccgtttg ttaagctaat actcaggtca aaacgctgat
    10921 ttcacaacct cttccttaaa cggtattcaa aagcttgcat ctgcagtaat aagaacagcc
    10981 aagtaaaaac ataagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    11041 tttttcacaa cctcttatgt accttgtagg actcgaacct acgaccggac ggttatgagc
    11101 cgtctgctct aaccaactga gctaaaggta catcataaat aaaatttatt tttgttactt
    11161 ttcaactata gcacgaatga caaactctca caagtttcaa aatataagta aaataatatt
    11221 gttattctta tttcttttac ttgtgtcata actattttgt tactttaatt tttatttgct
    11281 tattttagaa aaaatgttat tcttttattt attctcaatg ttaggagtga ttattttgcc
    11341 atttattgcg attttattcg atttagttgc ggcagcagct tattttctcc aattaaatta
    11401 tcaaacctca ggaattctgt ttattggaat gatttttcaa ggtgtgatta cccttttgct
    11461 tcttgtcatg atgctcagct ataaaggaaa aagatatgcc cgtattcaaa caaaagtatt
    11521 tgttaaatat gtttccattc gctatgggat catcattctt tcctttttag tgaatgcgat
    11581 cgtcctcttt ttatatgtat tgaattactt agatatcaat ccattgattt tttctcgata
    11641 gataagacac attcagcgga acagttcctg cttatcagca gaaaactgtt ctgctttttc
    11701 atttttcatt gatctattct tcttttattt tcctagcatt tcatttgtga tgtatgcaat
    11761 aacatgttat cttttattat atgaatcgtt gaggagtccg ttacatgatt acgttggtga
    11821 gagttttgtt ttgggtgcct gcggtcgcac tggtcgcaag tattgtttat cttatgaatt
    11881 ggaacaaaga acggttttat ttagctattt tgactcttcc ggctatctac tttatgtgga
    11941 aagtttttaa ttacaactac tttgaacccg atagtgtttt tatagaagaa ttatcaggac
    12001 tggttttgtc tttattgatc gtcattcttt atttgatacg tttaaataaa aaacattgag
    12061 tactattgat tgcatgtgta gctttctata gtcattataa ctattctact ataatgaaag
    12121 gtgattaaat gagtatcgaa gaagctattt tactggatac tttacgttgt cgtctgaatt
    12181 tcttaagaac tggccgatct ttatctcaag tagctgtttc tagcgcgtta agtattcctt
    12241 tattcaaata tcgtaagtac gaagaaggag aagaaattcc aagcatcatt gaattaatct
    12301 atatcgctga cttttacgat atttccgttg attacctaat tggtcgctca gaaacagcaa
    12361 gcaaactgaa gtgatttttt ccagccttgg tgctggtttt ttcatttaac gtctaaggtt
    12421 aacaatctat atataaagga gacatttcta tgcgattatg gcatgaaaaa ttgatttcaa
    12481 agcttcctcg tcaacagctt ttaggacaac atcgggaatg ctgtgcatta agaggaaatg
    12541 gctggggaaa gaaacatgct actgtggatt atgtttttca ctattctcct tatagattat
    12601 tccaatatca tcagcttgtc atggatgaaa tggaaaaacg tggttacagc ccagcaccag
    12661 agtggaaaaa tcctatgtat cgtggcaaat catgcgaagc attttttgat ttacctactg
    12721 tagagcgcaa cgtgccgatt tatccggaac acgatgaagt ttatctagca gaatgtttag
    12781 caaatttgga gcaaaaagga atttatctta cctaatataa ctagaaaagc cttctatctc
    12841 ctcttacttc attaaaaagc tttaaccatc ttagatcaag ctaaaatgtc gtaagtttta
    12901 gggttatctt catcctcacg tgcaaaacta aaagcgtact ctagttttgg ttcatcgcca
    12961 acttccctag acataaatga acatctcctg acgtggttta aaagtaccgc atcttcgacg
    13021 tctgtatacg atgcacagca cagattgctt cttttgatct ttcttttgat ttttctgttg
    13081 cttcttctac accttcttcg attactacat cgatgcttac aattgcttct gcctaatatt
    13141 cccatgcatc gtcgatttaa gcctctgtag tcactgtgat cgtttccaca ttttaactcc
    13201 tctgtcgtgg cttcagttga ttttgtgatc agttcagttg ctaagccaga gtcagaacaa
    13261 cctgccattg ctagcgttaa tattgatagt aaaaatagtt tttttatcat aaggttcccc
    13321 ccctgaaaat agactaaaat aataacgctt tctttttttg ttttcagacg aaaattaata
    13381 atttctaaat attctttaaa aaagcttata actattatgc cttttttaat atataacaca
    13441 gttattttta aaaataagaa tgctaaatca ctctacaata acggcgaaag cacaaaaaat
    13501 aaaaaaatta ttacgttatt aaaaaaacag atgaaagatc aggttttatt agatctcatt
    13561 ctttcatcac ttgaagggat ttaatgaact actttttcct ctttgtcgtt tttatatatt
    13621 cgtattcctt caaacagtag gatagcctct ttcaatgctt gatatttgaa ctgtccgtta
    13681 cttaaaaatt tagatgtgcc gccctcttcc tagaacatat tcttttttac tttttcatta
    13741 attgacagac caatcgaacg aaactgtaaa tttcgatagg ataatgattg gtcaaactca
    13801 ttcattttca agacatctcg attcatattc aagataggaa tctttttatc aacatcactt
    13861 tattcgtatt ttcctctacc ataaaagctt cacgctccac aaaattgttt tatacactac
    13921 tattcagccc attcaacgac caatgaattt tttcgtacaa aaaaaccgtc ttttctttcg
    13981 aaaagacggt taaaagtcaa tttttattaa gctttaacaa tgtttgttgc ttgagggcca
    14041 cgttgaccat cttcaacatc gtaagacact gcttggcctt cttctaaagt tttgaagccg
    14101 tcaccttgga tagctgaaaa atgtgcaaat acatcatttc catcttcacc agtgataaag
    14161 ccaaaacctt tgtctgcgtt gaaccatttt actgtaccgt tattcataaa taaattcctc
    14221 ctgatacgta tcaatgtacg ttttttcgtt gcaataaata tttgataggc aaaaggagtt
    14281 ttatctatgt agatatatcg ctcaaattac gtttcaaaat agattactca tttaatatat
    14341 catgcttttt ctaataacac aaggataatg caatgaaata taaaattcat tgtcttctat
    14401 tgtcttcgaa tcgaagaaaa agttgaataa ccgcgattga ttcacttttt gttttcttgt
    14461 ttatttgctt ttatctgcaa cttttgactt taaaatttct taactatgaa aacctttcaa
    14521 ttggtggtat acttataaat ggaatttcgt tatcaacgcg ttcgttgcct ttcagcaagt
    14581 atttaaagaa aggagaatgg ctatgacatt ctataacaaa gatatacaat atcgagtaac
    14641 cttggataca gatttgaatc tatttgtcgt ttttgacaaa gaaaatacca atcatgtagc
    14701 gacaggtgtt acgatcgaac aagcagtgca agaattaaaa aaatctgcat aaggagtgga
    14761 aaccctcata tggacgattc tcgttcttct ctttaaacgc atctactggt agataacagt
    14821 agatgcgttt tgtgtttgtt ctaaattgtt cactacaaat aaggtagata cttcaaaata
    14881 aaaaattact tctttttcat ctgttccctc agtcgtgcag gacaataaat aaagaggact
    14941 agaaaatagt cctctttatt agagatacaa ccctttactt tctaaaaatc gttttgcttg
    15001 atgcaggtca taaaattcat agtctgcttt attggattca tataaactta catctacttt
    15061 caacttatgc ttcccattat gatcgggcaa ccctatcaaa tgatctttga tgagaaaaga
    15121 caaaatgccc gctcgccttg ctgcttcgat ccctgcaatc gagtcttcaa atacaagtgc
    15181 ttcacttgga ggaatattca tttttgaaca agctttcaaa aagatctctg gttccggctt
    15241 tccttttttt acatcatccc ctgaaatgat ttgatcaaac aaggcatata gattttccat
    15301 ttttaaatac atttctactt ttacccgtgt attagacgaa gccacgattg tatggattcc
    15361 tctttctttt gcaaaagata aaatttcttc tgctcctttt ttcgtatata ctctcttatc
    15421 ttctttcacg attttttctt tatattgatt gattgcttct cgccatttga tatatggaac
    15481 atcttctccg tacaaaatgc ccatttgttc tcttatcgcc atttctgttt ggccagttag
    15541 taaataatag acttccttac gcatttcata atcatacttt tgtgctgatt ttacataagc
    15601 acgataagct aaacgtccag tttctaataa aaggccatcc atatcaaaga tgaagagctt
    15661 gattttattc ataatgctct cccagtggtt tttccttact tatcgcctca tatagacctt
    15721 gcatgtaagt gagtcccatt gcacgatcat acaggccata tccaggcata gccacttcac
    15781 cccacacggt tctaccatga tcagggcgta taggaccgtc gaacccttct tgcaccaacg
    15841 ctttcataat atgatacata tcaagggagc cttctgtaga gggatgtgcc gtttctttga
    15901 actttctttc tcctaaaaac tccacgtttc tgaagtggac aaaatggatg cgcgaaccta
    15961 cctcatggat gatttcaact aaatcgtttt ccggatttgc ccctaaagat cctgtgcaca
    16021 gcgttagtcc attatatgga gaatctactg catttagaat tttcttcaag tcgccaagat
    16081 ttttcgtcac tctaggaaaa ccaaaaagtt cccatggcgg atcatctgga tggatagcca
    16141 tttttacatc tgctttttcg cacactggaa tcactttttc taaaaaataa gtcagatttt
    16201 caaataattc ttcttcggtc attccttcat acagtttttc cagctgagcg aattttttca
    16261 accgttcttc ttcccaaccc ggcaatttga atccctttga ctggctgtgt atcaattgat
    16321 acatttctcc tggctccatc tgatcgacga ttttctgatc gtataaaaga gaatagcttc
    16381 catcgttgtt ttcattaaac aaatcagttt tcgcccatcc aaaaattggt ttgaagctat
    16441 aacaaatcaa tcgaacccca catcgagaca agtttttgat tgttcgtata tagttatcaa
    16501 tatagtaatc tcgttcatct gtccctgcct tgattgcatc gtggatcgct acactttcaa
    16561 ttccaagcag ttccagtcct tggctctcaa cagatgcttt caactcctca atttctgaaa
    16621 tctcccaaac atctccaggc attttagtca gcaaagttcc aattatccca tttattcccg
    16681 gtatttggcg aacatatttt aatgggatcg aatccccttt ttcaccatac catctaaatc
    16741 cccacttcat cttttcagcc ttccttctaa aaatcgtcct catctaattt tttttcttcc
    16801 gctgaagcat gccaaacagt taaattatct tttccttgtt tgacaatttg ttccaccgct
    16861 tcttctatcg gtgtgtgaag catctgatga ttgattgctt ctaacaacat tggcagattc
    16921 acgcccccta tgacataagt ggctgctttc ttttcttcag tcagttcatt gactgccaag
    16981 agcgcttgat tgtacggact tgcgctaatt agatcaacaa atactagcat gggttctgct
    17041 gtgttgatca tagttaattt ttctttgacc ttctctccga atgtttggat gtcctcccca
    17101 gactctaagc acattgtttc aatatggctc gatgtcccga caattacttc cgccgcatct
    17161 ttcagtcctt cgctcaactt accatgtgta acgatcatga tttccaacat tagtcttctc
    17221 actccttgtt ttgcactttt tacttatcga aaaagagcaa taatctgatt ggcattttgc
    17281 caattaaatg ttttccctcg taaatctgtt ttggtcaaat agaagaactg cgcttgcttt
    17341 tttcttgcac taaaaatata attgacgttt ttttctttca acttatcaac ttgtttcagc
    17401 cactgttctt tatttgaaaa ataatttccc gaaaactgta gcggtgtatc tgccccaaca
    17461 gagtctagat aaaaaagaat agcctctttt ttcatacccg atctcacgct gtctaagccc
    17521 ttttctccat aacagccttc atcaacaaac gcatacgcta cactcggatc atcaatcgta
    17581 cggatcattt ctagcatcgt taatatggat gaagtatttc gaataaaagt ttgtctgcta
    17641 ggccatccgc gagctgcttt tcccaataag aaaaagtagc tcccaaatgc tagtacagtg
    17701 atacctgttt atacggatag cagatcgaat acgtgatgag gatttttgtt atatatcatc
    17761 attccccacc aacctaaact gaacaacaaa atgaaggaca ataatataga gtagattgtt
    17821 tttttccttt ggtctttcca atcaaacatg aaataacttc ctaatgcatg aacgggtgta
    17881 tcgtaataag tacagataac tttctctgct ttttcgatat ctccaacata aatatttttt
    17941 gatcggtagg ctagcgtatc gtaagcaatg actgtcacat ctgttctcat actgtaaata
    18001 tctgatacta atgctgaaag aaaacgctgt ttactctttt ttgtacggcg cttacggtaa
    18061 atatagcgat aacgaaattc ccaatcacga aacagctcgt tcttcttcat tttttcgccc
    18121 tctttatctt ttttctaagc atttgtttat gacaaatcaa acttgttcaa tacatctttt
    18181 aatcctgttt taggtgcaga tggagtggct tgaaaataaa tattctttac gccatattgt
    18241 tcattcattt ctttcaattt ttcagcatct gatttattta aataaacaga tttcgtcact
    18301 aaaagatctg tatcaggatt tttttgagca atccctccaa tattcaattc ttccatgggg
    18361 attccgtgtt ttactaagtc gtaaatcgtt tggactgttt ttgcgatcaa aatacaattg
    18421 tattctttaa attgatattc atcccaatag aatttagctt tctctggtgt aatgacaatt
    18481 gttttttgtc ctgtacggct tcctgcattt ttgtaaagat ctctcataaa tttatctttt
    18541 gctacaactt cgtctacaac aaaaatggaa ttcactccac ttttttgaga taacgttacc
    18601 attacttgcc catggatcaa acgctcatcc acacgcgcta aattgactac acccatctta
    18661 ttcacgttcc cttcttttta taaaataccg attcctgcta gcacaattaa tattccagtc
    18721 agccataata atgcttgtgt gactttcaat ccttttttcg tatagaacaa gtacacaccc
    18781 attacgacag ctaaaggtaa tatccctggc acgatttgat caagaatttc ctggataaca
    18841 aactcacgtc cggatatcgc aaatttcaat gaagattcta cttttacata gtttcccgca
    18901 agcaccccca tcataaacaa ccctaaaact gatagcattt caatcgctgt ctgaacacca
    18961 gtgttttgca ttaggcctgt agcattacgg cccatagtga aagcttgtct tgcgaaaaat
    19021 acccctagta aaacctgata caacccaaag cagataaaag gaaaaagtgc gcctaaagga
    19081 cttccctgtt gtgcccaagg taccgcgatt gcgataaaaa tatattggac ggttcctgaa
    19141 tcgattgcat ctccgattcc tgctagtgcc cccatcaatc ccgctttcgt gttatacatc
    19201 aagtcatcct gcatcaagat ctctttttct tcatactctt cttctgctct ttgctgttcc
    19261 atagaagaca ttaggccagt aatcacaccg ccaccccagc ttaattgagt gttgaagaac
    19321 agtaactgcc gtttatatgc tgcttttaaa gagtcttcat ctttataaag ttttttcaag
    19381 attggcatca ttccgtatag tagtgatggc gccaagtaac gttcaaaaga gtgaggaatt
    19441 tcgtttgcaa aatgccatct cagataggct tttgttacat cttttttcgt gatttcttca
    19501 ggagcacctg tttgattatc cattatttca gccatttccc attcctccta aattaaaatc
    19561 cgtcatcata gtcatcttcg tcatcaaaag atgctgaatt gttattgctt gtggcaggct
    19621 gtacttgttt ccctttttgc gactggacaa agatcaaagc gataataatc ccaaaaatgg
    19681 cataagtaac catcgtaata tctaaagatt tcaaaacaac actcataaaa taagcaagga
    19741 agaaaaagac cagataatct tttcgaccga taacgtatac agttgtcgcg ataccgattg
    19801 ccgctaaccc tccacctact acttcaagaa tgtggataac gactgttccc tctaaagctt
    19861 tgattacatc agcgatcaat ggcgccccta aatataaagc aatgaaaaca agcggaacga
    19921 ataataaaaa tgaggcaacc gttggataaa gaataattga tcgggccata gcttttgtat
    19981 tcaaatcttc tacagctttg tctgtatatt tacctaaaaa agtattgatg aaaaaacgaa
    20041 actgatacaa gtaacttccg agcaatccga ctggcaccgc aacggcaatc gctgcttctg
    20101 gcgacagatc ccctaataat gcgactggta ctgcgatagc tgctgcaata gatggttcag
    20161 ctggcattga tcctcctgga gaaaaaaccc ccatataaat catttgcaaa gcggctgtaa
    20221 caatcatcgc ttgtgcaact tcccccatga tcaacccgac gactaatcca gtcatcaaag
    20281 gagaaaaacg taaggtcaac gtggctcctc cacctaacca gcgggacata accgcggcta
    20341 cccataaagc aatcaacaaa ctttgtacta cacttaatgt ttccatgtgt cctcctcctt
    20401 cagatctttc tgatataacc ttccagttcc atcattgact gtatcagtga tttctgtgtt
    20461 acctctatag gaatcaaatg cactttctct ttcgaatcaa taaattcagc cattttatct
    20521 attatctgtt tatctttcgg aaataatccc atttgttcta gagaaaccgg cagttctaat
    20581 tcccgataca aaggatataa ctcgtctatt ttttcccagc gaccttctag tgccagttga
    20641 tagaaaatcc catacgctac tttttctcca tgtagaaaac gatggctttc cggaatatat
    20701 ttactgatag cgtcatgcat agcatgtgct gccgcatttc ttgcgtattt atctcctaat
    20761 cctccaacta aaccagcaac agcaaagata atctctgaaa gctgtttgaa ctccgatgtc
    20821 agttttcttt gtgccatatc ttggattgct ttagcagaat gattcaaaat ctcttctttg
    20881 cagatcttcg cagtatcttg tgctagcttc aaaaatgatt catcttgcaa aaaatcttgt
    20941 tctaagatga gctcagattc ataccatttt gctagtgtat ctgctaaccc ggcaatgaaa
    21001 tatggaacag gggaatcaat cactaattgg ggatctgtta taaaaaaagc agcttgtctg
    21061 taaaagtgtt ctgttttccc ttctgcttgt ccattctcct tatacatgac agcaagaggt
    21121 gtccatggag cacaattact agctaaagta gggatgactc cgaaatcgat tcttgcaaga
    21181 ttggcactat aacccactag atcggtcagt tttcctccgc caacgccaac tacgaaatcg
    21241 atttcatttt tttgatgatt gtagctattc gtctggcttc agcataacta cattctccag
    21301 aataacgatg ataaaaaaat tgaatcgttt cgtcattcaa aaatactaat ttaggtagcg
    21361 ccttttccca tgataccgtt ccgtgggtga ccagtactct tttcgcgccg tatgatttca
    21421 agagatcaga aacttttgaa ataccacctt cttcatataa ataaaattga gggcctgttt
    21481 tcacttttaa accgttcacc attttcttca tcatcctttt atatagttgc tctttgtctg
    21541 gacagaacgt tcaggaagaa ggtgctgact gatctgttcg acatctctga cacatttttc
    21601 acccattgct tctaaacatt cccttgtgta ggccgatata tgtggcgtaa tcactacttg
    21661 ttcaaacgct aaatacggat gatcttgacg tcccggttct tcttctaaaa catctgtggc
    21721 atatcctgcg atctttccgc ttttcagacc attaatcatc gcttcttcat caactagagc
    21781 gcctcttgca ctattagata gataaacatt ttctttcatc tgatcgactt ctttatcaga
    21841 aatcatgtga tagttctctt ctgtcaaatt cgcacataaa caaatgatat ctgattgttc
    21901 cagtaattct tgaagttcta ctttttttgc accaaagctt tcgatgtata aatctgattt
    21961 gtaaggatca taagctagaa catcacagcg aaaaccgtta cgcaaaattt cgattactcc
    22021 gcttccggta tttcctgcac caatcacacc aactgttttg ttaaacagcg tcctaccgac
    22081 gaactccgcc cgatcttccc atcgatcgtc cttgactctt gcttgtgctt gtgtcgtgcg
    22141 tcgaagcatg gacaacaaat tagttatatt attttctgct acagcatttc gttcaactaa
    22201 agcaggtatg atagatacga ttgtctggtg ttcttttgct gcttttaaat caatattgtt
    22261 gtaaccaata ccatgtcggg aaatcaatag cagttcatct ttgttttcaa aaaaatctcg
    22321 tcgaaaaaat ggcgtaacac ttgcaataat gatattgaat cctttaagta actccgctag
    22381 ttcttttcct ggaatctctt gatcaacagt aaagaaatga acgtctccaa ctgactttaa
    22441 tctttccaag tggtgtggaa agactcttcc gaaactgctg gaattaacga ttgctatatg
    22501 ataatctggc attttactca cccttttcaa ctatttcatg tccgccaaac ccatttctta
    22561 gtgctgaaac tacttttgct gaaaaactat cttcttcctg tgaaagattc ctcatgaaca
    22621 aactagttgc aattactgga actggaacag ccatttctag cgctgtttca accgtccatt
    22681 ttgcttcacc tgatgcagaa acgattcccc gataattact taaattggga tgttcgttta
    22741 actgatcttc catgatttcc atcagccagc cgcgaatcac cgaaccatga ttccaaacac
    22801 gtgcgacttg ttccaagtca aaagcatact cacttttttg tacaagttgg aatccttcac
    22861 cgattgcttg catcattcca tattcgattc cattatggat cattttcaag aagtgtccgc
    22921 ttccggatgc accagcatac agatatcctc tttcaattgc tacatctttg aataattgtt
    22981 ccacggatag aaaagtttct ttatctcctc caatcattaa gcaggcttga tgtcgtgctc
    23041 cttcgattcc tccagaagtc ccacaatcca agtaaccgat ttcatttttc tgaaactctt
    23101 ctgctcgacg gattgaatct ttataataag agtttccgcc ttcaataata atgtctcctg
    23161 gtccgagttt tggaaaaagt tttcttaaca tgttttcggt tatctctcca gcaggaagca
    23221 ttaaccagat gatttttttc tgctcaaaag tagctagaaa ttcagagatc tcttgataag
    23281 caggaatgtc attttctctt gcctgactaa cagctgtact tgaaatgtcg agtccttgta
    23341 ctttgtgatg gtgctctttt aagtttaaag ctaaattcaa tcccattttt cccaaaccaa
    23401 ttacaccaat ttccatgata gcgcctccat ttcttatacc ctcattatac aaaaaaattt
    23461 ttttgtaaat agattttaca aaaatatttt ttgtttgatt ttttcctact ctccttgtat
    23521 ctttaacatt aatgagaacg gaaaaaatga taatcttatt atgaaaatga ggaattctca
    23581 tgagcgtctc cataagacta caaatcaaaa caatgtacaa cgacttcagc ccaaaagaac
    23641 aggctatcgc cgattatatt ttagaaaatc caagcaaagt aagtcatagt cctattagca
    23701 atttagcaag tgaactgggc attgctgact ctactttttt tcaatttaca aaaaaacttg
    23761 gttataatgg atttaaagat tttaaaatgg ccatgttgat ccaagaaaat gatctatctg
    23821 ctatttctat ccacgaaaat attcagaaaa cagatactga gctcactatg gcgcaaaagg
    23881 tgttcgagtc tagtatttca actttaaatg acacaaaaaa gttgttacaa caaaaggatc
    23941 taaaaaaagc ggctactatt atcaatgaat ctaaacgtgt ctattttttc ggtgttggcg
    24001 gttcagagat tgtagcaact gacgcttatc ataaattttt gcgttcccct atcgcaactt
    24061 ctcacagcac ggattaccat attcaattga tggaagcttc tttattgacg gaaaaagact
    24121 gtgctgtttt gatttctcac acagggcaat caaaagaaac aatccatatt gctgaaaccg
    24181 taagaaaaac tggagcaaaa acaatcgtta ttactagcca agctaattct tcccttgcta
    24241 aacttggcga tgtcgttttt atttctattt ctgaagaaac agaattccga tctgaagctt
    24301 tagcttcccg tatttctcaa ctaagcattt tggactctct atatgtgatt ttgatgtttt
    24361 acaacgaaaa agaatcacgt cgttctatcg ctaaagttcg acatactatt tctgaaatca
    24421 aagaatagca ccataacaga aaaagcgaaa aatccataaa acaaaaaaag ccactaccaa
    24481 atatttggta gtggcttttt tggttctgca agggtggtac agaacatcaa tagtcggtgg
    24541 gttagaacgt atgtcatcgc atctttggag aaggttgtat tggtattatt cgagttactt
    24601 gttagatgcg ataacaaagt tagtatacag tagtaaaaaa tttatctttt ctttttttta
    24661 ttatttttgt ttcttatttt ctaaaaaatt aaaaaaaatt ttggtgaatt aagtatttcc
    24721 ttgataacac tggcttttgg cctcatttta ggttatcatt tgtaatttta tttcacagtt
    24781 gatatgattg ttatgtaaaa taaaaaagtt taggatacat cgaatttttg gggaaattct
    24841 tttgggggta tcctaaatct tttctattat attcccatat tttctattat tggtctagac
    24901 gtatgatggt aaactttgta cctatctgat tgctgaatca ttaattgctt atcattagcc
    24961 atctttgttt caagttcgct agatgatcat tttattcaca tcctaacaaa cttgtcacat
    25021 ttatagattc ttaatctatt tatcatacaa ttataaaaaa ggaggtgctg cttatgaaag
    25081 ctatccatat ttctttgctt tttttcgcta gtgcattgat tttatttgct attcatcttt
    25141 gttcccagcc ttatatcaat aacaacggaa tattagtgga accagcattc ttttgcttac
    25201 cactcggctt tttgtccctt cttgcctccg caggcttcgg atttgttgct ttttttaaac
    25261 gatcaaaaca acaaatctga cttataaaat ttacgtaaaa gagatcgtga caataatcgt
    25321 gtgatattcc caaatttcga ataaaaggtg ccccttgcca tctctaactc ctcagtcaca
    25381 agttacaata ttcataaacc atgaaaagct tgtttattga aattgctcct tgtgacttgg
    25441 agctaagtga cagattacaa cctcttttgc atcataagac tttactcctt gaaaaacatt
    25501 actaactttt gatatgtttc ttgcgcttgt ttattgctga aatcaaactg atattcatgg
    25561 ttcaaaacag catttttctc cgcataatct acttttgtga ctttcacccc cgctttctca
    25621 aatacagaag ttacttcttc catttgtttc gtaaacgtat ttgtatttcc atccgttaag
    25681 aatgtgtttt gcgtccaacg atccgcatgt ctgaccaagg ctaaactttt gaccttctct
    25741 gattgttcaa agtccttatt atcaaagtaa gcccatccac atttttcata aagaaagcta
    25801 gagattttat cacttacttg agtaagtgca gaaggatcca acagaccaga caaagaaata
    25861 aacttcgtaa tctgcttgtt ctccaaagtc ggatcaagat gatcagcttt tttaatttct
    25921 tcattttgaa gtgctaacaa atattctcca ctaatttgtg cacctgccga atttccacca
    25981 atcgaaatat tcgaccaatc tgcttgatct tttatctctt ttttagctgc ttgaatggct
    26041 tcgttcaatt ggataactgg tgtaggatgt tttgcttccg gtgatcgttc ataatctata
    26101 tttacgatga tttgctgcgt gtctgaacat agatattcca aataatcacg acaatcattt
    26161 ttatcccctc ctacatatgc gcctccatgc atccagaaaa gaatcggtaa ttttttcttt
    26221 tccactttcg gtgtgtagat atccatatta ttatttttgt actttgaagg ataggatatt
    26281 tcttttttta tatcgatttg ttcgattttt tgctggaaat catctggtcg ggcatatact
    26341 tgttcttttt cacttgtgtc aaattgtttt ctaacaagca agctaatcgg ctttggtgtg
    26401 atttgattaa tcaaaaatac agtgactaaa ataactacga tgataactgg tactgcaatc
    26461 actattcgct tgaaccattt cattcattta ccctctcttt cttcgccaaa tccatgtgat
    26521 aatcgtgcta acagataaaa atccacctga ataaatgaga agcgaatgtt tcttttctcc
    26581 agttttcggc aacattcttt tttttgcatc tgtctttttt tgtactttca ctgtcatctg
    26641 aatcggttgc gtatcatcac ttgattgatc ctctcgaggt tcattctttc ccttattgtc
    26701 tgcattctct gtcggatctt gcttttttgg tggagctact gggatttttt tgtaatagaa
    26761 aataatagtt ggtatcgtct cattaaaaat cccttcagtc ggtccttcaa catttgctaa
    26821 ttcgtatcct tcgaatgtct tttgctctgt catataattt tctccataag ctccgtaaag
    26881 ctggatttcg tcctggtctt tgagtagact tttattgttt tcatccacat actttacgtt
    26941 tatctgatca tggaacacta ctttcaaaaa gtccgttaca tgtttcgtgt aggcagcatg
    27001 gtcaacttcc tgtgcctttc catgccctgc tccttctact aataaaagat ctttataccc
    27061 tcctgataac tcatataatt tttgcgcgtt agacacaggt actacatcat ctgctgttcc
    27121 atggataatc aatttaggca atgaatctga cattccaatc gtaggtagag cagcttttgt
    27181 atccaaatgc atctcctgat caaaatagta ttgtttcaaa aggcccataa ataaatcttc
    27241 atgctcttga tccccaatga tgtctaactg attttttatg gcatccggta gtgcgggggc
    27301 tactgtctgg ctgtacgtaa aacgtagttg ttcatcaatt gatgcaaatc cgcaattttc
    27361 aataattcct ttgacattga caggaatatc tttcgctaat acagacatca tcgtagctgc
    27421 tcccatcgaa ccaccataaa gtaaaatctt ttgagatggc cgctctcgaa cttcttgatt
    27481 tatccagtaa aggacgtcat ctgactcata ttgtccatag gtcacataat cgcctccgct
    27541 attaccagtc gctctggaat caacaaataa taaattatac ccggccttat aaaaatcatt
    27601 gtattcttct gtaacgatcc cgttattcca attccctcga aatccgccat ggatcatgac
    27661 tgttttatct gaaccttgat cgacataata tgcatataca tccatcattt tgccatcttg
    27721 tttgtttagt acagtttgat gaagttgttt tgtatttgga tagaagcctg tgttttctgt
    27781 aggcgttgtc ccatcatctg tacctccaag catcgacatc aacacactgt tttttgcaaa
    27841 agcgatatcg tacaatagat aagtagcaat cagatgtttg ataatctctt tatctacacc
    27901 ataatcatta acagcaagct cagtgactaa taactctatt aattttgact tgatgtcttc
    27961 tgttggtcca ttaagtattt ctgtcatctg tttaccgaag taattcaaat attctttacc
    28021 attgaaattt tcacctttac cgatatcatc attttctgtc ccattctctt tgtcacttac
    28081 tttagcagaa gaggattcgt cgtttgacat ttttgtagct gttgcttctt ctgtttgttg
    28141 gttatctgtg gtagcggtcg tttcttgatc tgtgctagta acagatgtct caaccgttgt
    28201 ttcagtattt gtttctgttt gtgaagtact cgatttcgtt tcatttccag catcagtagt
    28261 ttctgtggtt tctacctccc gagattccga ttcggaagaa atgctctctc catatactgt
    28321 ccattgcggg acgcttgtac ttacagcaaa taagatgacc aacgctccct ttaatacttg
    28381 ttttgttctc aagctattca ctccttttta tgtttctagg agaattatat gtaaacgcaa
    28441 acatttttta tacaaaaaat aaatggttta tcccataaaa atagaacagg attattttct
    28501 cattttacgt aataaagcag aatggataaa ccagtatcca ttctgctttt tttaaacgaa
    28561 gaaacaactt attgctccaa aaatgtcaga aatcgtttat cccaaatttt ctcgtaaaaa
    28621 gcgattgttt cctttgcttt cataaaagga ttatcataag ttgtcgttgc tcctctacac
    28681 atcataagct ggtatcctaa tccatgagca aacttcacag tcgtatctat acaatactct
    28741 gcttgtgccc cacataattc gatcgaatga cagtccaatt cagttaataa aacactcaaa
    28801 ttagtcttat aaaatgaatt cgcatgggtt tttctcacaa aataactgtg ctcaggaata
    28861 gataattccg acaatagctc ccatggtttt gtttgtggca ctaattgagc atcttcgtgt
    28921 tgaacgaaaa taactggttt cccaatcgtt tcgtatgttt tgattctttt attcactaat
    28981 gctgtcactg tttctaattc gtgtatcggc gaagaatcaa aacatacccc tttttgtaaa
    29041 tcgatcacta ctattgcgtc ggctgtgttt gacatacttg tgccttcttt cttttttctt
    29101 gatttaatag tatttcttat ctagctagtt ccattatacg tgacaacatt ttcttatttt
    29161 acacctctaa atggcacttt tcgactattt cttcatgtta ttatttcata aatacttagc
    29221 tattggagga atgtatgatg attaaattgt atacaggttc tggctgctcg tcttcaagac
    29281 gcgcaaaacg ttggcttatc gtccatgata taccgtttat cgaacagaac ttaattcgtc
    29341 aaccactaac caaaaatgaa tttatccact tactaaaatt gacaaacaac ggcgtgaccg
    29401 aaatgctctc tactcattct attagttata aaaatcttgt tcacagaata gatgaactca
    29461 gtttgagtga actatatatt gagattcgta catccccttc tttattgaaa aaaccgatta
    29521 tctacgacca atctgcaaaa cttttgactg gatttagcga agataaaatc aggatgttcc
    29581 tttctaaaac taaaagaaag caagagctca acgaactctt gctttctggt catcttgtcg
    29641 attttaaaga aaataatctt tttgcaatgt cttaattttg gcataaacaa tctgcttttc
    29701 ttaattaacg aaatccaatt tttccaatat ggatctattt ttctcattcc tgatttttat
    29761 gatttcagct ttcagacgcg cccaatcatt ttttgtaggt tgtccattcc agtaaaatat
    29821 ctgtgtattc gtttgatcat atgccttttt gtcgatagga taatcgctaa tgacaatatc
    29881 aggtaactga tcagcggatg cactatattt tattgggcgt tcagtcatag ataatatcct
    29941 tttctttgcc cataattcct gtgttttacc atactttgat tgaatataga atatgacagg
    30001 ttgttcgtgt tttataaaaa tatccctgac taacatacag tattgaaaaa tcaaccgtct
    30061 gttcttttca taataagtat taagctctgg aattctgcga gctaccccac taaaaaactg
    30121 ttgcatgatt ggatacatca ctggaaatat atgctgaaaa tctctgtctt ctgctgattt
    30181 tccaaaagca tccaccttct tttttgtccc aaaaaccctt gaatacaaat ggatcattgc
    30241 caaattgaca tatagataat ttttttctga agttgtaaaa caaatcttga agtttttttc
    30301 aaaattttct acaatagacg attcaatcca gccccaactt tcttgtactt tatctggagt
    30361 atccaacaaa tcttcttcca aataggtgtt cattacacca aacataaaat aaaggtaagc
    30421 ttgctctgtt ggagtgatgt gattcatcga ccagaagttt gcttcaaaaa aaggctctag
    30481 tatgtaatgc gtaaactctt cttttgttat caatggatgc acaaatttgt tcatctcttc
    30541 tgggacatac gtaatcgagt ggccttgttt cattcgttgc aacgaaattc ttagtaggct
    30601 gatccatttc tttctatcta tactacgcgt atatggcgca tgtttcgcaa atttttctaa
    30661 tagttgacgt ttatttttta tacttgataa tatatcagaa tgattttcgt cgaatgactg
    30721 ccaatacatc aagtgaaaaa agtatcggat acggtattct tctccttcta tcggattacc
    30781 ttttttcaag ctgcaagata aatgatagtt tttcaaaata gtattaagtt ctcttaattt
    30841 tctttgaatc gtacgcaaac ttatataatt ttttgactaa gattttctaa ggattgataa
    30901 gtacctttgt aaagtgaatc cagtaaaata taaccaatcg ttccctctaa aagtatagaa
    30961 taaactttac tcagtaaaaa ctgagaagaa aactcgactt ttatcatctt gttttctgaa
    31021 agaaaagata atttatattc tggatacctt tcgcaaaacg attctgcttg ttgaatcacc
    31081 gaattcatct tagccagggt gtagtttaga tctaaagcga tatccttcac cacatataat
    31141 ttctcgtctc tttcataaca atagttcaaa aattgatata cttgcctttc attgatatca
    31201 ataaattctt caaacaaagt acatactcct ttttatctat tatttagact tattccgaca
    31261 gctacatgaa taacgtacaa atagattcgt tactgatttt tcagagatac taccatgcgc
    31321 agtgtgcatt gtcaattatt cttgctctta ttttccattt gtgttcctgt tatttcctgt
    31381 ttctcaaata tgttagtaaa caaaaataga tctggcaaag ctacaacttc gccaggcaat
    31441 acagcactct cgaatgaaat attcactcaa aaatctatcg ttttattcaa ttttagatct
    31501 gttgctttta ttctttattt cactcgatgg aagagagaac gaaaatagct tgctcctaca
    31561 atcaaaatga gtaacacacc catccatgta attagcttgt tcaaggttct actaccagtt
    31621 tctggtaaat cgctttttct attatctgtt tctttcgctt tttcttgatg atcaattcca
    31681 cgagaaacaa aatcaggctg ttttagcaca ctttttaggt tgctccctgc atttctcttt
    31741 tgttccttag ttggtatgat aggtaccaaa tcaagttcat tatttacttt tcctgtgctg
    31801 ctttcgctgc tcgtgcttcc tgtgctactc tcactactca tgcttcctgt gctactttca
    31861 ctactcgtac tctccgtact gctttcgctg ctcgtgcttc ctgtgctact ttcactactc
    31921 gtactctccg tactgctttc gctgctcgtg ttttctgtgc tactttcact gctcgtactc
    31981 tctgtactgc tttcaccgct cgtgttttct gtactgcttt cactacttgt gctatccgta
    32041 ctgcttggcg gtccaacaac tgtccaactg cttggtttca aattctccgt gatcgtcatc
    32101 tgtccatcat aaaaaacaat caaatcaact ttatctttac tcttgatctc atcaatcgat
    32161 agttctgcag aaatcattat tgtatactca ataccagcgg tccattgtac gtcttttaaa
    32221 taaacttccc caataacttt ttctgaactt ccattaccat cccaaccata tgttagaaca
    32281 gcatgtacat cactagcatt cacgcttttg tttggtttga ctggaatagt aatgacttgt
    32341 tgtgtcatgt cataaacaat ccgatcagtt ttcacagtaa atgaaagatt ctcatttgcc
    32401 cgttcatttg cagaagtact gattccaatc attgctgcaa ttatactcag cccaagaata
    32461 acaaataata cggtcgctgt tttcttcatt tctatcactc tccatataat taccttctat
    32521 ttgataaagg aattatataa aatgaaaatg atgagaacac gacagtcagt tgtgaaaatc
    32581 taacaaaaga tgtcgtgtat tcgcttgaat ttgtttactt tttcgtttaa tcaatgacag
    32641 acttaaaaaa gcaagaccga tatcagcctt gctttcttca tcttttattg ataaacagca
    32701 gtgtatttct tcatccgctt tgttttatat gcatagagta acaaaccaac aatcaagact
    32761 aacaagaata atgtagttga tatagatgct tcagccccaa agtttccccc agtaattatt
    32821 gtattttgcg attggatcag agtggagaaa aaaccttgtt cttgcgttcc gctgactggt
    32881 atacccaaaa tagctccttg tgtccaattc caagtaatat gagcaccaat agccgtaaat
    32941 aaacttcctg tactgtagat aagtgcgctg aaaaagcaac ctgctaaaaa gatattgaaa
    33001 actgccagaa atgtcatgtt aggattcaat ccgtgcaaaa gagcaaataa tgcagcagat
    33061 ccaatcattg ctattaacgg accaaatcta agcatctgat gctgaaaaaa gccacggaat
    33121 aaaagctctt ctgatgcccc cactgcaata aataaaataa aactcgttat cagcggcaca
    33181 atgtttgcgt ttcctgaatc taaccgatac gcccccgtca aatataatgg actagtaata
    33241 agcagaaaca ctccaattcc aacaacgaga cctcccgcta aataccccca gtcttttcct
    33301 cgaacagaaa caccataatc ttctattctt tttccttcga ccttactagc aagaaataaa
    33361 gctagtaaaa aaacagctaa atgtccgacc gtaccggcaa ttgctccaat ccattcacta
    33421 tttccaataa acatatccgc aagtgcatta ttgacaagaa ttcctaaaac aaaaccaaca
    33481 aagactatta aaacagatcc aatcaaccga ataatcctga tcatctttca tccctccttt
    33541 atattaagtt taacaaccct tttctttctt gtctcgtgaa aactatgtaa atgttctcca
    33601 tcatagagag aatagatttt tacgaactaa gaatatctac agcaacctta cgaaaatgaa
    33661 agactgtctc cctaattctt ctgttagact gtttttaata aggaaaggat gggaaatttt
    33721 ttgaaaaaac aactactaat tatgtgcctt tcagcatgtg caattttact attctcaggt
    33781 tgtagcaagt ccaatgaaac gattgcaacg tttaaaggtg gaacacttac agttcaggat
    33841 ttttatgata acccaaaagt taaatcaaat aatcaaacac tattaaaaaa aatgattgtc
    33901 ttcaaagcat ttgatgatgt atacggaaaa gagatttctt caaaggaagt aacaaaagaa
    33961 tataatgaac aaatcaaaaa actcggtcca aactacaagg aacaattaaa agcagttggc
    34021 caaacagaag agacatataa gaaactcttt aaacagatgc tggctttcca atacggtttg
    34081 aaagccaatg taaaattaac agataaagat ctagacactg cttggaaaga attctatcca
    34141 gaagttagta cacagatcat tctattttcg actaaagaag aagcagataa agcaaaaaaa
    34201 gaagcaaatg aaggagaaaa tttctccaag cttgttcaag cttatggaaa aaataaaaca
    34261 ttaaaggaaa ctgatggaaa aatgaatttt gattcaacca atccagagat tcctactgaa
    34321 gtaaaaaaag cagcatttaa actaaaaaac ggggaagttt ctgatatcat tcctgtaaca
    34381 gatccaacga cttatcagca aagctattat ctagtaaaaa tggtaaaaaa acaggataaa
    34441 ggttctaata aagacaaata caaatctgag ctagaaaaaa ttgctaccga agcaaaaact
    34501 cttgatacgg aattcatgga taaaacaatc cgtaaagtga tgaaaaaaga caatgtaact
    34561 attaaagatc cttatgtaaa aaatatattc aaataaacaa atttgaccca attggggatg
    34621 tggcaaaatt tgagaagtga tttttggaaa ttccttctta tttttgaggc aaaccacttt
    34681 tgccacaact tcatcggggc ctttttttat ttgtaccaaa acaaatcact ggatttgtta
    34741 caatcatgag gatataattt aaaaaagttt tttatttcta tcacatgaat caatgcgcca
    34801 ttaagtagat tctttcatgc ttccctattt tttcacttgc cgtatcgctt caaaaacaac
    34861 tatcataaac tcaaactcga ctgaaaagtt attttaaaaa agactaaaaa tatcccttct
    34921 ggacttgttc ttcttattct gttatactag agaaacaaga aagatatttt tatgcgacac
    34981 ttttcatttg tttttatgaa atttattcaa aactggcgta acttcagagt tacaaggatg
    35041 tattagaggt tagggaatac atcgtttaaa tgagaaataa gtaatagtta gtaatagtca
    35101 cccaaacttt aaattaatga atattgaatc gtcagatagc ctcctcgagt tttcgagaag
    35161 gctatcgttt attttatgga gaaattgatt cagaagtaat cagcgacctt ttttagttca
    35221 ccaatcgttt ctgcttggaa agtttgcacc caatcttctt ggaagcccct cactgcttta
    35281 tcaatcgagg gcatctctaa tacttgatct agtagctctt tacctaacgt tatcgcatga
    35341 gctccagatc gaatggctgt agtgacttgt tcacaatttt tgaaactagc tgctaatatt
    35401 tttgttggtc tttgcgtacg ttcgatctca gtccgcaacg aatcaatcac tttcatagca
    35461 tcaatgcctt gattttgcat cctgttataa taaggtgcaa tataatctgc tccattatta
    35521 atagctaaat acccttgtaa ttctgtgtaa atagctgtac cggtaatcaa ataaccttct
    35581 tttttcaact ttttgatcgc ttgaaggcct gcttgattca ccggcacctt gataaacgta
    35641 tcttttccca gttcttctat gataactcga gcatcctcta tcatttcttc ttccgttctt
    35701 cctattgtct gaatatggat aggaatctgt ccgattattt ttttgatttc tttcatatgc
    35761 gagaagaagt ttgacgctcc ttcttttttc agaattgtag gatttgttgt tacgcctgtt
    35821 aaattcaaaa actgttgaca agaataaata ctttttgtat ttgctgtatc aagcatgatt
    35881 tccatatagc tttcccccat tctgatcctc ttgatcgatt ttaatgattg tcccctttct
    35941 taaatgcggc atttccttat cttctacata cattgttcct ggaagttccg gctcatgaga
    36001 tccatcaaat tttatcgtga tatgtcctaa ttcattcaaa ttagtttgga caacatctcc
    36061 tactgctgtg attacataca tttgtttatc aaaacagagc ttcatatcac aagttatttc
    36121 ttttcttacc tgattcaatt caatcttata acaataatct gttaatgttt ccggtgcttc
    36181 atctccaaat aaaatcatta ttttttcttt cctgaatact tctgcttcta taccaacatc
    36241 tgttacaacc gtttcaaaga tcgtcatctc tgttcctcct cgtaaaatat catttgtcta
    36301 atagttttta ttcaaaatgt ttctagatta taaaatttat tgaataacac attcctaacg
    36361 gaatttgttt cgatattgtg tgatttctca gagtacaatg gatgtatgga
//
LOCUS       DACEUF010000021        35882 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.022, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000021 DACEUF010000000
VERSION     DACEUF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.022"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..1152
                     /locus_tag="IXG20_000995"
     CDS             <1..1152
                     /locus_tag="IXG20_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609208.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IXG20_000996"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IXG20_000996"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ1609209.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IXG20_000997"
     CDS             2728..3003
                     /locus_tag="IXG20_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ1609210.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IXG20_000998"
     CDS             3009..3776
                     /locus_tag="IXG20_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ1609211.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IXG20_000999"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IXG20_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ1609212.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IXG20_001000"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IXG20_001000"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ1609213.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IXG20_001001"
     CDS             7920..8222
                     /locus_tag="IXG20_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ1609214.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IXG20_001002"
     CDS             8225..9388
                     /locus_tag="IXG20_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1609215.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IXG20_001003"
     CDS             9414..12842
                     /locus_tag="IXG20_001003"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ1609216.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IXG20_001004"
     CDS             12863..13987
                     /locus_tag="IXG20_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609217.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IXG20_001005"
     CDS             13977..14414
                     /locus_tag="IXG20_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1609218.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IXG20_001006"
     CDS             14398..14736
                     /locus_tag="IXG20_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ1609219.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IXG20_001007"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IXG20_001007"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ1609220.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IXG20_001008"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IXG20_001008"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ1609221.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IXG20_001009"
     CDS             15893..16936
                     /locus_tag="IXG20_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1609222.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IXG20_001010"
     CDS             17095..17823
                     /locus_tag="IXG20_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ1609223.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IXG20_001011"
     CDS             17939..18184
                     /locus_tag="IXG20_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1609224.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IXG20_001012"
     CDS             18181..18399
                     /locus_tag="IXG20_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ1609225.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IXG20_001013"
     CDS             18535..19074
                     /locus_tag="IXG20_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ1609226.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IXG20_001014"
     CDS             19067..21340
                     /locus_tag="IXG20_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ1609227.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IXG20_001015"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IXG20_001015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ1609228.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IXG20_001016"
     CDS             complement(22513..22767)
                     /locus_tag="IXG20_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ1609229.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IXG20_001017"
     CDS             complement(23167..23436)
                     /locus_tag="IXG20_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609230.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="IXG20_001018"
     CDS             complement(23906..24361)
                     /locus_tag="IXG20_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ1609231.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="IXG20_001019"
     CDS             complement(24499..26427)
                     /locus_tag="IXG20_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1609232.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="IXG20_001020"
     CDS             complement(26624..27457)
                     /locus_tag="IXG20_001020"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ1609233.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="IXG20_001021"
     CDS             complement(27649..28353)
                     /locus_tag="IXG20_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1609234.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="IXG20_001022"
     CDS             complement(28376..29002)
                     /locus_tag="IXG20_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1609235.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="IXG20_001023"
     CDS             29328..31703
                     /locus_tag="IXG20_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ1609236.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="IXG20_001024"
     CDS             complement(31727..32218)
                     /locus_tag="IXG20_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ1609237.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="IXG20_001025"
     CDS             32405..33325
                     /locus_tag="IXG20_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1609238.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="IXG20_001026"
     CDS             33479..33823
                     /locus_tag="IXG20_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ1609239.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="IXG20_001027"
     CDS             complement(34013..34513)
                     /locus_tag="IXG20_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1609240.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="IXG20_001028"
     tRNA            35041..35124
                     /locus_tag="IXG20_001028"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
     gene            complement(35448..>35882)
                     /locus_tag="IXG20_001029"
     CDS             complement(35448..>35882)
                     /locus_tag="IXG20_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ1609241.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11868 a         6262 c         7405 g        10347 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta gtaccaaccg
    35461 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    35521 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    35581 tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    35641 gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc tgttacagca
    35701 ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac cgtttcttgt
    35761 acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg aacggctgct
    35821 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    35881 ta
//
LOCUS       DACEUF010000022        34811 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.023, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000022 DACEUF010000000
VERSION     DACEUF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.023"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            591..1280
                     /locus_tag="IXG20_001030"
     CDS             591..1280
                     /locus_tag="IXG20_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1609242.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IXG20_001031"
     CDS             1267..2475
                     /locus_tag="IXG20_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1609243.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IXG20_001032"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IXG20_001032"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ1609244.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IXG20_001033"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IXG20_001033"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ1609245.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IXG20_001034"
     CDS             complement(6578..7273)
                     /locus_tag="IXG20_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ1609246.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLTPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IXG20_001035"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IXG20_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ1609247.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IXG20_001036"
     CDS             complement(8472..8663)
                     /locus_tag="IXG20_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ1609248.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IXG20_001037"
     CDS             complement(8684..9574)
                     /locus_tag="IXG20_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ1609249.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IXG20_001038"
     CDS             complement(9561..10328)
                     /locus_tag="IXG20_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1609250.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IXG20_001039"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IXG20_001039"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ1609251.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IXG20_001040"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IXG20_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ1609252.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEVQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IXG20_001041"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IXG20_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ1609253.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IXG20_001042"
     CDS             14944..15282
                     /locus_tag="IXG20_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609254.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IXG20_001043"
     CDS             complement(15450..16208)
                     /locus_tag="IXG20_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1609255.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IXG20_001044"
     CDS             complement(16226..17044)
                     /locus_tag="IXG20_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1609256.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IXG20_001045"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IXG20_001045"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ1609257.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IXG20_001046"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IXG20_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ1609258.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18173..19507
                     /gene="dnaA"
                     /locus_tag="IXG20_001047"
     CDS             18173..19507
                     /gene="dnaA"
                     /locus_tag="IXG20_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ1609259.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19705..20835
                     /gene="dnaN"
                     /locus_tag="IXG20_001048"
     CDS             19705..20835
                     /gene="dnaN"
                     /locus_tag="IXG20_001048"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ1609260.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21064..21306
                     /gene="yaaA"
                     /locus_tag="IXG20_001049"
     CDS             21064..21306
                     /gene="yaaA"
                     /locus_tag="IXG20_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ1609261.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21293..22417
                     /gene="recF"
                     /locus_tag="IXG20_001050"
     CDS             21293..22417
                     /gene="recF"
                     /locus_tag="IXG20_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ1609262.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22414..24360
                     /gene="gyrB"
                     /locus_tag="IXG20_001051"
     CDS             22414..24360
                     /gene="gyrB"
                     /locus_tag="IXG20_001051"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ1609263.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24380..26851
                     /gene="gyrA"
                     /locus_tag="IXG20_001052"
     CDS             24380..26851
                     /gene="gyrA"
                     /locus_tag="IXG20_001052"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ1609264.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDRAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27087..27386
                     /locus_tag="IXG20_001053"
     CDS             27087..27386
                     /locus_tag="IXG20_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ1609265.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27436..27948
                     /gene="ssb"
                     /locus_tag="IXG20_001054"
     CDS             27436..27948
                     /gene="ssb"
                     /locus_tag="IXG20_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1609266.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27974..28210
                     /gene="rpsR"
                     /locus_tag="IXG20_001055"
     CDS             27974..28210
                     /gene="rpsR"
                     /locus_tag="IXG20_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ1609267.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28353..30326
                     /locus_tag="IXG20_001056"
     CDS             28353..30326
                     /locus_tag="IXG20_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ1609268.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30332..30784
                     /gene="rplI"
                     /locus_tag="IXG20_001057"
     CDS             30332..30784
                     /gene="rplI"
                     /locus_tag="IXG20_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ1609269.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31058..32425
                     /gene="dnaB"
                     /locus_tag="IXG20_001058"
     CDS             31058..32425
                     /gene="dnaB"
                     /locus_tag="IXG20_001058"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ1609270.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNIIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33205..34497
                     /locus_tag="IXG20_001059"
     CDS             33205..34497
                     /locus_tag="IXG20_001059"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ1609271.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRILTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10604 a         6770 c         6880 g        10557 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggttgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtgtcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga cttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17821 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17881 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17941 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    18001 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    18061 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    18121 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    18181 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18241 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18301 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18361 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18421 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18481 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18541 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18601 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18661 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18721 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18781 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18841 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18901 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18961 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    19021 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    19081 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    19141 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    19201 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19261 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19321 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19381 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19441 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19501 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19561 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19621 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19681 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19741 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19801 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19861 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19921 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19981 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    20041 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    20101 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    20161 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    20221 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20281 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20341 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20401 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20461 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20521 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20581 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20641 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20701 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20761 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20821 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20881 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20941 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    21001 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    21061 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    21121 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    21181 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21241 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21301 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21361 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21421 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21481 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21541 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21601 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21661 agtaaaaggc agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa
    21721 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21781 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21841 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21901 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21961 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    22021 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    22081 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    22141 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    22201 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22261 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22321 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22381 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22441 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22501 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22561 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22621 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22681 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22741 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22801 gttgtaaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22861 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22921 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22981 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    23041 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    23101 ggcgggatca aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    23161 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    23221 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga
    23281 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23341 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23401 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23461 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23521 ttcttgctgg aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc
    23581 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23641 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23701 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23761 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23821 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23881 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23941 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    24001 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    24061 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    24121 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    24181 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24241 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24301 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24361 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24421 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24481 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24541 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24601 catgggtaaa taccatcctc atggagaccg tgcgatctat gagtcaatgg tacggatggc
    24661 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24721 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24781 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24841 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24901 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24961 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    25021 tggaccagat tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta
    25081 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    25141 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    25201 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25261 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25321 cgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25381 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta
    25441 tatcgaacat caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga
    25501 agcaagagca cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25561 tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt
    25621 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25681 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25741 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25801 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25861 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25921 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25981 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    26041 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    26101 tccagaatac gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc
    26161 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    26221 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26281 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26341 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26401 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct
    26461 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaaaatcaag aagtattagt
    26521 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26581 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26641 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26701 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26761 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26821 aacagttgaa gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa
    26881 gaataaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26941 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta
    27001 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    27061 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    27121 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    27181 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27241 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27301 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27361 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27421 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27481 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27541 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27601 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27661 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27721 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27781 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27841 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27901 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27961 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    28021 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    28081 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    28141 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    28201 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28261 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28321 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28381 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28441 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28501 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28561 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28621 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28681 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28741 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28801 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28861 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattagatg
    28921 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28981 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    29041 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    29101 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    29161 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    29221 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29281 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29341 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29401 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29461 tcgttatcaa tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29521 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29581 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29641 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29701 caagtaaacc tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt
    29761 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29821 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29881 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29941 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    30001 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    30061 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    30121 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    30181 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30241 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30301 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30361 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30421 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30481 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30541 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30601 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30661 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30721 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30781 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30841 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30901 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30961 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    31021 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    31081 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    31141 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    31201 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31261 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31321 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31381 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31441 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31501 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31561 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31621 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31681 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31741 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31801 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31861 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31921 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31981 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    32041 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    32101 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    32161 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    32221 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32281 cgatgatgga aacgagccaa aaaatgacaa catcatcgaa gtcatcatcg agaagaaccg
    32341 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32401 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32461 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32521 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32581 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32641 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32701 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32761 tttgaaattg ctccttgtga gtcagagtta aacggcaggc tcacaacctc tttcttaaac
    32821 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32881 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32941 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    33001 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    33061 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    33121 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    33181 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33241 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33301 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33361 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33421 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33481 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33541 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33601 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33661 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33721 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33781 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33841 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33901 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33961 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    34021 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    34081 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    34141 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    34201 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34261 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34321 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34381 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctt aacattttct
    34441 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34501 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34561 gataataata acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt
    34621 ctcttgaaca ctgtttattc agaataaggg actgtgacaa aaaccgtgtc acagtccctt
    34681 tttccgaaca aacggtgttc aaaagcttgc atctacggca ctaagcccaa tcagatgaaa
    34741 aatatggagt gctattttta tctgattgtt cttactgctt gatgcagagc agctttttca
    34801 caacctctcc a
//
LOCUS       DACEUF010000023        33209 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.024, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000023 DACEUF010000000
VERSION     DACEUF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.024"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..159
                     /locus_tag="IXG20_001060"
     CDS             <1..159
                     /locus_tag="IXG20_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAQ1609272.1"
                     /translation="IKNHEKFLIRNYKISGGGCYLLECRFTSKKSMNSFLEQLNEYAN
                     YKLSIVID"
     gene            complement(540..878)
                     /locus_tag="IXG20_001061"
     CDS             complement(540..878)
                     /locus_tag="IXG20_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAQ1609273.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
     gene            complement(1013..1414)
                     /locus_tag="IXG20_001062"
     CDS             complement(1013..1414)
                     /locus_tag="IXG20_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609274.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            1526..2146
                     /locus_tag="IXG20_001063"
     CDS             1526..2146
                     /locus_tag="IXG20_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1609275.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            complement(2237..4906)
                     /locus_tag="IXG20_001064"
     CDS             complement(2237..4906)
                     /locus_tag="IXG20_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1609276.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(5144..5332)
                     /locus_tag="IXG20_001065"
     CDS             complement(5144..5332)
                     /locus_tag="IXG20_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ1609277.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            5602..5964
                     /locus_tag="IXG20_001066"
     CDS             5602..5964
                     /locus_tag="IXG20_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1609278.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            6016..7698
                     /locus_tag="IXG20_001067"
     CDS             6016..7698
                     /locus_tag="IXG20_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ1609279.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            7815..9851
                     /gene="recG"
                     /locus_tag="IXG20_001068"
     CDS             7815..9851
                     /gene="recG"
                     /locus_tag="IXG20_001068"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ1609280.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            9900..10901
                     /gene="plsX"
                     /locus_tag="IXG20_001069"
     CDS             9900..10901
                     /gene="plsX"
                     /locus_tag="IXG20_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ1609281.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            11023..11265
                     /gene="acpP"
                     /locus_tag="IXG20_001070"
     CDS             11023..11265
                     /gene="acpP"
                     /locus_tag="IXG20_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1609282.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            11533..12537
                     /locus_tag="IXG20_001071"
     CDS             11533..12537
                     /locus_tag="IXG20_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609283.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            12538..13482
                     /locus_tag="IXG20_001072"
     CDS             12538..13482
                     /locus_tag="IXG20_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609284.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            13482..14444
                     /locus_tag="IXG20_001073"
     CDS             13482..14444
                     /locus_tag="IXG20_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1609285.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            14471..15385
                     /locus_tag="IXG20_001074"
     CDS             14471..15385
                     /locus_tag="IXG20_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1609286.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            15411..17192
                     /locus_tag="IXG20_001075"
     CDS             15411..17192
                     /locus_tag="IXG20_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1609287.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            17540..18226
                     /locus_tag="IXG20_001076"
     CDS             17540..18226
                     /locus_tag="IXG20_001076"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1609288.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            18246..21827
                     /gene="smc"
                     /locus_tag="IXG20_001077"
     CDS             18246..21827
                     /gene="smc"
                     /locus_tag="IXG20_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ1609289.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            21836..22645
                     /locus_tag="IXG20_001078"
     CDS             21836..22645
                     /locus_tag="IXG20_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1609290.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            22657..23655
                     /gene="ftsY"
                     /locus_tag="IXG20_001079"
     CDS             22657..23655
                     /gene="ftsY"
                     /locus_tag="IXG20_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ1609291.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            23842..26109
                     /gene="gshAB"
                     /locus_tag="IXG20_001080"
     CDS             23842..26109
                     /gene="gshAB"
                     /locus_tag="IXG20_001080"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ1609292.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(26197..26649)
                     /locus_tag="IXG20_001081"
     CDS             complement(26197..26649)
                     /locus_tag="IXG20_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ1609293.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
     gene            26799..27749
                     /locus_tag="IXG20_001082"
     CDS             26799..27749
                     /locus_tag="IXG20_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1609294.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            27742..28701
                     /locus_tag="IXG20_001083"
     CDS             27742..28701
                     /locus_tag="IXG20_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1609295.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            28698..29453
                     /locus_tag="IXG20_001084"
     CDS             28698..29453
                     /locus_tag="IXG20_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1609296.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            29476..30429
                     /locus_tag="IXG20_001085"
     CDS             29476..30429
                     /locus_tag="IXG20_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1609297.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(31073..31369)
                     /locus_tag="IXG20_001086"
     CDS             complement(31073..31369)
                     /locus_tag="IXG20_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ1609298.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            31725..32081
                     /locus_tag="IXG20_001087"
     CDS             31725..32081
                     /locus_tag="IXG20_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1609299.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            32091..32990
                     /locus_tag="IXG20_001088"
     CDS             32091..32990
                     /locus_tag="IXG20_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1609300.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        11011 a         5876 c         6956 g         9366 t
ORIGIN      
        1 attaaaaatc atgaaaaatt cttgattaga aattataaga ttagtggagg gggctgttat
       61 cttttggaat gtagatttac ttcaaaaaaa tcaatgaaca gctttttgga acagcttaat
      121 gaatatgcga attataaatt atctattgtt atagattaat aataaaacta gcagatggta
      181 aaaatactaa tatgcgagac acacattcaa ccataactat ataacttatc aatttaaaac
      241 taaactgatg gaaacaagta aggcaaaaac atttatattt tgccctactt atttttttaa
      301 ttaaattcat tgatgattca tatttagaaa attctatgaa ggccaaatga gtatcctgtc
      361 acttttttag ataaatagca aaaattttca gcgttaaatg acaaataatt agaaatattt
      421 gtgccattta aatataaaaa tgacaccaag tatatcatga acgcacaaaa agaccaacag
      481 ggtataccct gttggtctaa ataaatgtca aattccgttc tcaactgtgc atattcattc
      541 tatgttccat gtaccccatt atcctcctga atactcattc caatgttagt taatttttct
      601 aattcttcta cgcttagata agcgccatat tttacaattt cctcatcaat gacaataatc
      661 ggtaaacttt tcaaatcttt tgtctgcaac aagttaagaa ttttagggtt gctaataaaa
      721 ggcaagatag agtcacttaa aaaatataaa tttgtttcta tctgtttatt cttaatccga
      781 ttataaaact cttttagcat cgtatcctca tgtaatgatt gaaaaacgaa gagtccttca
      841 tcatcacttc tatttaagac atagtaagaa attatcattt tcctattgtc aatcctcccg
      901 cagtgcaaga gactgaccct acaccaaatg tattataaac ttgcatagct aattttacac
      961 tgtaatattg tcttttccca gtaaatgtaa aatacattcc tccaccataa ccttaagaat
     1021 tagctagatt tactgtttga gtatatgatt ttgcactacc taaactcaag cgtgtagaaa
     1081 tacgatacat cgccgaattg ggacttgtta taatatattc tgtccgcccc tgcgtattag
     1141 taattttttt agtagatgcg gttaaccgca atacatttgc agaaaaacgt gttttcggat
     1201 cttgaggctc tatcttttga gtgtaaaaag tatctttatc actaaatcct ttggatttga
     1261 agtaactttg ttcttctggt gttaattgac taaaagtttt tgccatttca cttgtttgtt
     1321 caaccatcgt gctagcttta actatatttc cagaagtccc aataccaata aagccaaata
     1381 acagaataaa aagtgcgcta aataattttt tcatagtaat cacttcctaa ttcattaaat
     1441 ttttaggaaa acgctttcta aatctcgatt atacatctcc ttttaaaaat gtacaccgtt
     1501 attttttgaa tattcagact ttttcttgtt cttgataaat gcaacagatg aaatattaag
     1561 tgtaaataaa gttttttata ctgtaccagc tgaaaagatg tctcgtgtca gttcggatat
     1621 taacacaata actagtagaa taggtctgac taaagcaatg catatggtac taaaatacaa
     1681 tcaagaagat cctcctgtgc ctaaaaccta tgaagcaatt gacttaatta ttgtcgatga
     1741 aattgatcgt ttaaagcttc agcatttaga acagctaaga gatatttacg atagaaatga
     1801 tctggcaatg gttcttatcg gtatgcctgg tattgaaaaa agattgtctc gttatccgca
     1861 gctttactct cgcataggat ttgcacatga atttgataac cttagtaagg acgaaacaca
     1921 tcatattttg gaatacaaat gggcagattt aggaattgat atcaagctag aagatttttc
     1981 tgattatgag gctatcacaa ctatcattaa gatcacaaaa ggaaatttta ggcttattca
     2041 tcgtttattt gcgcagattg atcggatttt aagaatcaat caaatggata agatcactgt
     2101 agaagttgtc gaagcagcaa gagatagttt agtcataggt atataaaaaa ccaccaaaaa
     2161 tcaggtggtt tttgtcattt aaagattgaa atttttgcca tttaaataga agagtgacac
     2221 agattccatc gtttctttat tttcttcgtc ttcttctgtg tgtatttcgc ggtttttctt
     2281 ctattgctgg atcaatcgtg ccataaaagg ggcgattgcg gtcaaggtac cagacataaa
     2341 gaataaacaa tgcaatacat aggacaaaaa ggattgcacc tatcgtaaat ccttcaggca
     2401 agtaactgaa agtgacagta tgtttacctg ctggaacttt tatcatcaag aatgcgtctt
     2461 tgaaattgct gaccttgact ggtttcccat caattttagc tttccatcct ttgtcataag
     2521 gaatagttgt caccaacatg gaatcttcag cggtatttac agtcccagaa gcttttctgc
     2581 cagtggcttc catttcgaca cctttttctt tgatctgatt cattgcggct tgataggcta
     2641 caacatctag accaacgact ttaggctcca tgaaacttac ttctttcgtt ccataaaaac
     2701 ttgcagtaaa tgtcactgtt gtctcttgag gataatagcc aagatcatag tattgaccag
     2761 aaatgttgat ttgcgttttt cgcgatgtac cattgacagt gatcgtcgca ctagaacttt
     2821 ctaattgtgc aaaattggtt gggaacaaac ttaagtaagc ttgtgtattt gcaggcacat
     2881 ggacttccca agtgagctct tttggctggt tatctgctgt ttctgagtaa gtgacgccag
     2941 tcgttgtctt tgtgatcgtc gtattcaact gattcttcaa agtaggtgta tagaatgtaa
     3001 aatactgttc atttaaacca gacaatgtat tgatcaagtt tgtctgactg gtcaaattat
     3061 ctgtcactgg ctgctcaaca tcattaacgg acaacggtgc agtaaatgct agtggcaatg
     3121 cattgacatt ctgatacagt gtatagtcgc cactttgacc agcttggcgg aagccatatt
     3181 tactgaacgt gctttcgttt ttagaaatgt tatatttgat tcctgtaaac ccatccatta
     3241 gcaaagtatt attgttataa cggatattca aatttgtccc tcttgaacgg aaacctaatt
     3301 tatccaggta agaggaagaa ttacgatttc ttatagaaga aaacaaactg ataccactgt
     3361 aaccatagtt gatcgaatcg tttgcagata ctggatccag attttccaag cggtaaaacg
     3421 agtcggaatc tttttttgtt tcatttacaa gagctttcaa gtcaggataa ggatcagtat
     3481 ataaactgcg ggaagcataa ttccaatcat caagaatgcc atggatcata ctgttcgtat
     3541 tgaccagtgc ttcagcagac atgatcaata ataacaatat actcagatga ttgaatgaaa
     3601 gttttttatg ctgataaaat cccacagcaa aagcataaat ccctaagaaa accactgtca
     3661 atacaaagga agtcatcgtc acataggtgt agcttccttc aggtttgatg ccccatgcca
     3721 ccgcaaagag agcagctacg accaaaatga ttcctaacaa cagcccacga tcgtcttgtt
     3781 ctatcttttc ccatcctttt cctgcaagca ggagcactaa gaaagagaac agataggcat
     3841 aacggaataa aaacatattc ggtgcatgca taccatgcca gaacaaattc aatggaacaa
     3901 gatagaaact tgcgataagt atggcgaaaa gactgccgta caaaaattta tttttcaacg
     3961 gtactttccg actggcaaaa taaaacagac agaggactaa tggtagcaga cctgcgtaaa
     4021 cgaacggaat cgatccgtat tttgtcgtgt cataaacacc aatcatattt ttcatgatta
     4081 gatccagata agaagtcgct tctgttttca acgtagtgat ctgactcagt gcctcgccat
     4141 ttgaccgaag atccagcact gctggcaata caatgatcat tgaagcgccg cctgccagta
     4201 aagaagtgat accataagga acaatcgttt ttttatattc tgaccagttt gtcaatagac
     4261 gggcgataaa atataagaaa gaaaatacgc caatcataaa acccatataa aaactggata
     4321 aaaacaagag taaataactg ataaataaaa gtttcggccg tttaagatcc ataacacgat
     4381 gaatccccaa aatcactaat ggaagataaa caaatgcatc cagccacatg atgatctccg
     4441 aatgtgcagt gataaatgag atcaatgcgt aactgacgct aagtgcaaca tgcccccagc
     4501 gaggaatctt gaacgtacgt gcaagaaacc aaaaagccaa tccggcacag ccgaccttaa
     4561 gcaaagtcag aacatacagt gcatctggca tcatctggtt tggaaaaaag aacaccagtg
     4621 gtgtgaagat cccgcctaga tagtaagaaa ttaaagataa ataattcaat cctagagaag
     4681 cattccatgt ataaaaaata ctttgctttc caagaagaac atttcggaag ctggcatgaa
     4741 aattagaaaa ctgtgaaaag gcatcacttg ccaaaatact tctgctgctt ccaggataga
     4801 tcccgatact tagataaaca agtgctaaaa tcaaaaacgg aatcaggaaa ctagctgctg
     4861 cgtagcgtcc gttttctcta aagaatgact ttaaattttt tttcatattt attttccacc
     4921 aaatttctat gaagttgtat tcagtttatc atatccttgt ttttctctgc ttaaaaagag
     4981 tctaattttc acaaaaaaat aaaatttaga ataaataact tcataatttg ttcgtataac
     5041 aaaaataaac gcaaaaaaag ggtaggaatc aaaagtccca cccttaaaaa tttttattaa
     5101 ataaaaaacc gttcactatc tgaacggtcc aagtatcatt caattaaacg cgttcaactt
     5161 ttcctgattt caaagcacga gttgacaccc aaactttttt aggttttccg tcgaccataa
     5221 cgcgaacttt ttgtaagtta ggtttaacag tacgttttgt tgagttcata gcgtgtgaac
     5281 ggttgtttcc actttttgtc ttgcgacctg taaagtaaca aacttttgcc atggtaattt
     5341 cctcctttgc cttgatttgg agcaatagaa taattcagct cataccagac taacatatca
     5401 tatagaatgg aaatttgcaa gtacattttc atagatacaa ttgacaattg agaagaataa
     5461 ttgtaaatta aacaaggtta tacctgaaat tttgcaaggt ttttgagtac tttgggtaac
     5521 tgccttttat ttcaagttgc gctatgataa aatatgatag aagaaaaatg ggagtttttt
     5581 acccgttaag gaggctcatt catggctgta aaaattcaaa cgtcagctgg aaccattgaa
     5641 attaccaatg atgtaattgc aacagttgtt ggtggcgccg cgacagatgt gttcggtgtt
     5701 gtaggaatgg ccagcaaaaa tcaaattaaa gataatatta ctgagatttt gcgtaaagaa
     5761 aattactcaa gaggagtagt tgtccgccaa gaagataatg gtattgctgt agatgtttat
     5821 acgattatca gctatggtac gaaaatctct gaagtttccc gcaacgtcca agaaaaagtg
     5881 aaatacaatc tagaggcatt attaggcgta actgccaatt cagttaatgt ctttgtacaa
     5941 ggtgttcgtg tactgccaga ctaggcagga gactggctgg taactcagca gtcgatctga
     6001 aggaggatta tctaggtgag tgtaacagaa ataagtgcaa gccagttcca ggaaatggtc
     6061 caggcaggtg cgaatcgtct gcaagtaaat gcagaatacg tcaattcatt aaatgtattc
     6121 cctgtgccag atggcgatac gggaacaaat atgaatctat ctatgactag tggagctaag
     6181 gcagtagtag attcaacctc agaaaaagta ggagaattag cagcaatgct ttcaaaagga
     6241 ttgctgatgg gtgcgcgcgg aaattccggc gttattttgt ctcaattgtt tcgtggattt
     6301 tctaagcaaa ttccagatgt tgtctcatta aatgcttccg atcttgctgc agccttcact
     6361 catggagttg aaactgcgta taaagcagta atgaaaccag tagaaggaac gattttgaca
     6421 gtagctagag aagcagcgaa agctggagaa aagaaagcaa aaagtactga tgatgtcgtt
     6481 gaagtaatga cagctgttgt aaaaggggca aagcgtgctt tagctaaaac tccagacttg
     6541 cttccagtcc taaaagaagt cggtgtcgta gacagtggag ggcaaggact gctgtttgtt
     6601 tatgagggct ttttaagtgc attgaatgga gaattccaag cggatgaaac atatgaacct
     6661 tctccagcag aaatggatga tatggtcaat gcagaacatc atcgcagtgt ccaaggtcaa
     6721 atggcgacag aagatatcca ttttggttac tgtacagaaa tcatggtaaa aatcggagaa
     6781 ggacctacag tcgatagtac attcgattac gaagaattcc gtaactattt agatggtata
     6841 ggagattctt tacttgtggt caatgatgac gagatcatca aagtccacgt gcatacagaa
     6901 aacccaggag aagtcttgaa ttatggacaa aaattcggtt cattagtcaa aatcaaagtc
     6961 gacaacatgc gcttgcagca tgagacgatt ttggaacacg atgaagaagt gaacgagttt
     7021 gcgaaaaaag aaacaccacg tatcccgtat ggtatcgtag cgatcgctgc tggtaaaggc
     7081 gtccaagagc tgtttgaaag tttaggcgct aattatgtta tcagtggtgg gcaaacaatg
     7141 aatccaagta cggaagacat tgttaaagcg attcaagaag taaacgcaga taaagtgatt
     7201 gtcttgccaa acaataagaa catcttcatg gcagctgatc aagcagcaga agtagcagat
     7261 atcccagtag cagttgttcc ttcaagaacg atttcccaag gattgacagc tatgctttca
     7321 ttcaatgatc aagcttcttt agaagaaaac aaagaaacga tgacagaaat gttgtctagc
     7381 gttgtgagcg gtcaagtcac tcacgcgatc agagatacct caattgacgg tgtagagatc
     7441 aaagaaggag actttttggg catgatcgat ggaaaaatcc tgatctctaa cccagatatc
     7501 acagcaacag cgttaagtac actagaacaa atgatcaatg aggacacaga gatcgtaacg
     7561 atcttggcag gtgatgaagg aacagttgaa caagcaaatg aatttgctga gaaactaaca
     7621 gaaaaacatc ctgatttgga aatcgagatc caccaaggag atcagcctgt ctatccttat
     7681 ctattttctg ctgaataatt tctgtgtcag aatatctttc taacacagta ttcattcata
     7741 aaaggccttc ggttagtgat caggtatgat cgctgaccaa gtcctttttt tgttaaggga
     7801 gggaggaaaa atttatgcgt tctttagatg atccaatcac tatgctggca ggagtcggag
     7861 aaaaaagagc agctagtctt gccagcttgg gcatccaaac gattgaagac ttactgactt
     7921 attatccttt ccgctacgaa gatattcagg aaagaaatct aaacgagatc caagatcaag
     7981 aaaaggttac tttgaaagga atcgtagtct cgcctcctgt gatgaatcga tttggttata
     8041 aaaaaagccg attgcaattt cgtatgatgc aagaacatga tgtgttcaac gtttcttttt
     8101 ttaatcaacc ttatctaaaa gaaaaagtca ttttatcgga ggaaatcgcc gtatacggta
     8161 aatgggatgc aaagcgtaaa gcattgaacg ggatgaagat tttaggttct caaacaatgg
     8221 atgacttttc gccgatatat cacgtcaaca aatcaattcg tcaaacgaca ttagttgatc
     8281 taatcaaaaa agggtttcaa gaatacggca cattgattga agaaaatctg ccaaacaacc
     8341 ttgtggagaa atatcgactg cttgaccgag cttctgctgt aagaagtatg cattttccaa
     8401 aagaccacga agaaaaccat caagccaaac gacgtgtcgt ttttgaagag ttctttttat
     8461 ttcaaatgcg tcttcaagga ttgaaaaaag cagaaaaagc agaaacgaat ggtttggaga
     8521 tcctttatga tgtccaaaaa ttgaaagaat ttactcaaaa gctacctttt gaactgacaa
     8581 atgcacaaaa acgtgtaacg aatgaaatct gtcgggatct tcgcagtccg cagcatatgc
     8641 agcgactttt gcagggagat gtaggtagcg gaaaaacagt ggttgccgcg attgcgttat
     8701 acgctaccgt tacagcaggt ttccaaggtg cgttgatggt gcctacagaa atcttggcac
     8761 aacaacatat ggaaagcttg caacagctgt ttgatcccaa tgaagtaacc atcgcattgc
     8821 ttacggggtc aacgaaagca aaagaacgtc gtgagctcct tgaaaaacta gagcaagggg
     8881 aaattaacat cgtgatcggc acacatgcat tgatccaaga tggagtcgaa tttcagaatt
     8941 taggtctagt tattacggat gagcagcacc gttttggcgt gaaccagcgg aaagtacttc
     9001 gtgaaaaagg ctggcgacca gatgtcttat ttatgacagc gactccgatt cctagaacat
     9061 tagctattac agcatatggt gagatggatg tatctatcat tgatgaactt ccagctggac
     9121 gtatcccgat cgaaacacgc tgggtaagaa cacctcaatt ggacagtgta ctggattgga
     9181 cgaaaaaaga actggcaaga ggacatcaaa tgtatgtcat ctgtccgctg attgaagaat
     9241 cagaagcatt agatgtaaaa aatgcagtgg aaatctatga aaaacttcgt gaactatttg
     9301 caccaaaata tgaagttggc ttgttacatg gtaaaatgaa gaatcaggaa aaagaagtaa
     9361 tcatggaagc cttcaaagac aaccagatgc agatattagt ttcgactact gtaattgaag
     9421 taggggtaaa cgtgccgaac gcgaccgtca tgctgatcat ggatgcggat cgatttggat
     9481 tggcacagct gcatcaactt cgtggacgtg ttggacgcgg aagtgatgct tcctactgta
     9541 tcttagttgc taatcctaaa aatgaactag gtgtggaacg gatgaaaatc atgacagaga
     9601 ccaccaatgg ttttgtacta agcgaaaaag atctggaatt acgtggtcct ggtgaagtat
     9661 ttggcttccg tcaatctggt ctgcctcaat ttgcgattgc tgacttggtg acggatggaa
     9721 atgttttgga ggtagccaga gaagaggctg cagccctttg gcagataaag gattggcaga
     9781 tgcttcctga gtatcagcct ttagcgcaaa gcctccaaat agaaaatcag gataatcgtt
     9841 atttcgatta gcagactgtg ctatacttta tactgatgac ggaaaaagga gtggaaacaa
     9901 tgaaaattgc cgtagatgca atgggcggag atcacgcacc tcaagcaatt gtagaaggcg
     9961 tggctttagc gcagcaagat tttccagaag tagaattctt actttacgga aaagaagcag
    10021 aaatcaagaa atacttaaca aatgaaaaaa acatcacgat tatccataca gacgaaaaaa
    10081 tcaatagtga tgatgagcca gtaaaagcaa ttcgacgaaa aaagactgca tcaatggtcc
    10141 ttgctgcaca agcagtgaaa aacggtgaag cagatgctgt attttcagca gggaatacag
    10201 gcgcgctact agcagcaggg ttgttcatcg taggacgtat caaaaatatc gaacgccctg
    10261 gcttgatgtc taccttgcca gtagtaggga aagaaggtgc cggtttcgat atgctggatc
    10321 ttggcgctaa cgcagaaaac aaaccagaac atttgttgca atacggtatt ttaggttctt
    10381 tttatgcacg taaagtccga ggtatcggac gtcctcgtgt cgcactctta aacaacggaa
    10441 cagaagcaac aaaaggtagt gaagtgacga aaaaagccta tgaactcctt cagaatgaaa
    10501 caagcttgaa ttttattgga aacgtagaag ctcgtgagct actcaatggt gtggctgatg
    10561 tagtggtcac agatggattt acaggcaacg ctgttttgaa atctattgaa ggaacagcaa
    10621 tgaatatgat gaatctatta aaatctgcta ttctaaatga agggatcaaa ggaaaaatgg
    10681 gcgctatgct tttaaaagac ggcttacgtt ctttgaaagc agaaatggat tattccaaac
    10741 atggcggtgc cgttttgttt ggcttgaaag caccagtcat taaaactcat ggagcaacag
    10801 gccccgatgc tgttcgctat acgatccgcc aaatccacac aatgcttgag acagatgtag
    10861 ttggacaatt agtcgaacaa tttgaaaaaa gcgaagaata gtatttggat atctgaagca
    10921 atccgttcca ttgaaaaaaa ggtggacatt cctgaaaaat gccagtaaaa ttagccatgg
    10981 tgtaggtaat tgacaagtgt atgattcttg gaggtgaaaa agttgactcg cgaagaagta
    11041 tttaacaaag tagcgaaaat tatttcaaat cattttgaga tagataccga taaagtgacg
    11101 gatgaattga acatcaaaga tgatttaaaa gctgattcaa ttagtattat ggaattcgtt
    11161 ttagagcttg aagatgaatt cggtacggaa atttcagatg aagacgctga acaaattgaa
    11221 acagtcggtg gcgcagtaga ttatatcagc agccatctta aataatttca aataaaagca
    11281 aagaaggata aaaagctgca tacagctttt tatccttctt tttttaatct ttttgacacg
    11341 taaaagtcat ttttctgata attctggcga taaacagaaa ttatgttgca aagtaaagcg
    11401 attttctata taattattaa taattcatta cacaaagata agaataacct ttgttataaa
    11461 acgctttatt ctgtgtaatg tcctttttta ttaagaaaaa cttccattta ggaagcagag
    11521 aggggtattc gattgtccga taaccaatta ttagaagttc agaacctaca tacaggattc
    11581 cgactcaaag atgactatta cgatgcagta gatgacgttt catttacttt agacaaaaac
    11641 gagattttag caatcgttgg ggaatctggg tgcgggaaaa gtaccttggc tactacgatt
    11701 atgggattac ttgatcctaa taacacaaaa attacaggtg agattttata taacgatctg
    11761 aatttaatcg acctgaacga aacgctctat aataaaatcc gcggaaatga tatcggaatg
    11821 attttccaag atccgttgtc tgcattaaat cctctcatga gaatcgaaga ccagatcaaa
    11881 gaaggacttt cctatcacac aaagatgaat gcagaacagc gtcaagctcg tgcactagaa
    11941 ttgctagaac aagtagggat tcctaatcct gcgagagttg gacgccaata tccccatgaa
    12001 ctatcaggcg gtatgagaca acgtgtcatc atcgcaatcg caatcgcctg caaacctcca
    12061 atcgtcattg cagatgaacc gacgacagcc ctagatgtaa ccatccaagc acagattctt
    12121 gatttactga aagaccttca ggaagaaaca aaaacaggga tcatcttgat cactcatgac
    12181 ttaggagtcg tagcagaaat ggctgatcga gtggcagtga tgtatggcgg acagttcgtt
    12241 gaagtagcag gtgtaaaaga actgtttgat catcctaagc atccttatac acgctcttta
    12301 ctgaactcta taccacaaga aggaaatcat gatgcagaac tccatgtgat cgaaggtgtc
    12361 gttccttctc tgaaaaacat gcctcgtaaa ggttgtcggt ttgcaccacg tattccatgg
    12421 ataccagcaa gcgatcatga agaagagcct atccttcatg aagtcgcacc gaaccacttg
    12481 gtacgttgct cctgctataa gcatttccac tttagagacg aaaaaggaga ggtgtagatg
    12541 acagaattaa tcacaatcaa agacctgaaa gttcattacc ctattcgcag tggattcttc
    12601 aatcgagtaa cggatcatgt atatgctgta gatggtgtgg attttattat cgaaaaagga
    12661 aagacatatg gtttagtggg agaatctggc tctggaaaat ctactacggg aaaagccgtt
    12721 gtgggattag aaaaggtgac agccggacaa atcatgtatg aaggaaaaga tgtaacgaag
    12781 cgaagcaacc gcaaaaaaat gggttataac aaggatgtgc agatgatctt ccaagattca
    12841 atgtccagtt tgaatcctaa aaagcgagtg ttggatatca ttgctgaacc catccgaaat
    12901 tttgaacggt taagcgatca agaagaaaag aaaaaggtaa aaggactgct agatatcgtt
    12961 gggatgccgg aagatgcgct atataaatat cctcatgaat tttcaggcgg acaacgtcaa
    13021 cgtctaggag tagcccgtgc tgttgctacc aatccaaaac tgatcgtagc cgatgaacct
    13081 gtatctgctc tggatctatc tgttcaagcg caagtattga atttcatgaa acgaatccag
    13141 caagaatacg gcctcagcta tttattcatt tcacatgatt taggtgtagt aaaacacatg
    13201 tgcgacaata tcgcaattat gtacaaaggt cgatttgtag aaatcggtac aagagaagac
    13261 atttataatg acccgcgaca tatctatacg aaacgtctgc tttcagctat tccgcaaatc
    13321 gatgtagaaa atagggaaag ccacaaagaa aatcgacgaa gagtagaaca agagtatatc
    13381 caaaaccaaa aagaatacta tgatgcgact ggtcgagtct acgatttgcg tacgatcaca
    13441 actacacaca aagtggcgtt gaaagatgga ggtgcaagct aatgtggaaa acgattctgc
    13501 gacgtgtttt attgatgatc ccgcaagtta ttattttaag tgttctgatc ttcttattag
    13561 cccaagcgat gccgggggat ccatttaccg gtttgatcaa tccaaatcag gatccggcag
    13621 tgatcgaaca gatgcgacaa gcagctggct tgaatgaccc atggtatgtc cagtacttcc
    13681 gttggatcgg aaatgctttg caaggcgatt ttggtcaaag tttcttatac aaacgctctg
    13741 ttgctgactt gatcggcgaa cggatcatca acactttata tctatcgatc ttgacagttg
    13801 tccttactta tttgattgct gtgcctctgg ggatgcttgc tggccggtat caaaattcaa
    13861 tcttggataa gattgtcgta atctacaact tctttagttt tgctgttcct ttatttatct
    13921 tcggcttgat catgctattt gtttttggtt atcgtttagg ctggttccca acaagtggat
    13981 cacaaacact aggtttgcaa ggtggattta tcactcaatg gatagaccgt ttgaaatata
    14041 ttctgttacc atcaattacc caagcatttt tggcgactgc cgtcacaatc caatatctgc
    14101 gaagtgaagt aatcgattcg aaatcgctcg attttgtcag aacagcccgt tccaaagggg
    14161 taccgacgaa taaagtattc agtcgacata ttttccgaaa tgcagcgttg ccgatggctt
    14221 ctcaaatggg gtatgaaatc acttcgttga ttgctggttc tgtagttatc gaaaagattt
    14281 ttggttatcc gggagtcgga aaactattta ttgattccat cggccaacgg gactataccg
    14341 tgatcacatc cttagtattg attttaggaa tcgcgacgct agtggggaca ttactttcag
    14401 atatcatcat gagtatcgtt gacccgcgta ttcggattca ataaaaccaa tcagaaagga
    14461 gaatggatcg atgagtgaaa aaaacaatac agcagttcaa gaaagtattc ctcctatggg
    14521 gatacgaatg attgctcgag aatttaaaaa agataaaatt gctatttttt cattaggttt
    14581 gctagtggtt ttattattag tgatctttat cggtgcgatg ctgaccgatc aagacaaagt
    14641 gatgtatgtt agtatcttcg acaaatacgc tgcaccagga acagtttcga cacaaggaca
    14701 aaagttcatt ctgggagcag atgaaggcgg acgtgatgta ttaggacaat tgatcatagg
    14761 tgctagaaac tctgttacga tcggatttgc gatcacgatc cttacttcga tcatcggggt
    14821 tggattaggg atcatctctg gctactacgg agggatcatt gacaacatct tgatgcggat
    14881 cgttgatttt atcatgattc taccgatcat gctgatcatt atcgtcttcg tatcgattat
    14941 ttccagatac aatgtctggt cctttatatt catcatgagt gcattttatt gggtagctaa
    15001 agcccgatta ttccgaagca agacattatc tgaagcaaga agagattatg tcagtgcctc
    15061 aaaaacaatg ggaaccaatg attttaaaat catgttccgc gaaatcatgc cgaatctaag
    15121 ttcattaatc attactaatt tgacgatgaa ctttgcggca aacatcggta tcgagacaac
    15181 tttgactttc ttaggtttcg gcttgccggc aaatgtacct agtttaggta cattgatcgg
    15241 ttatgcaagt aacggagaag tcttagtgaa tcggcaatgg atctggttac ccgcatcaat
    15301 ccttatttta gttttgatgt tgagtatcaa ttatgttgga caagcattca agcgttctgc
    15361 agatgcgcgc caacggttag gctagtatag aagcgaaggg ggaaatagga atgaagaaaa
    15421 cgatttttgg atttgcagcg ctgatgtctg ctgtcgttct tgcagcatgc gggggcggag
    15481 gtacagatac ctctggaaac aactctggag gcaacggcaa taacaaagag gaattaactt
    15541 ttccaattgc aacaaaaaat aatgatgatg caatcgaaga cggcgaatta gaagctgcgg
    15601 tcgcaacgga cagtcagttt aaaggattgt tccagtggga gttttaccaa gattcatatg
    15661 atgcagcttt tatggctcct tctcatgaac cgctatttgc aaacgatgac gatttcagaa
    15721 tcacagacga cggagctgca acgcttgatc tagatgaaga tacgaaaaaa gcgacaatca
    15781 aagtaaaaga caacgtcaaa tggtcagatg gtgaagatgt aacggcggac gatgtgattt
    15841 tcccttacga aatcattggg aataaagatt acactggtat ccgttatgac gacactttcc
    15901 gtaacatcgt aggtatggaa gaatatcatg atggaagtgc cgacacaatc tccggaatca
    15961 aaaaaataga cgacaaaaca gttgaaatcg aatataaaga agtaaaccca agcatgcttc
    16021 aatcgggtgg tggtatttgg tcatatgcag caccaaaaca tacgttagaa ggtgtagcaa
    16081 tcaaagatat ggaatcaagc gatcaagtac gtaaaaatcc agtgactttt ggcccttatt
    16141 acatgagtaa tatcgttgca ggagaatcag tagaatactt gccaaacgag cactactgga
    16201 atggtacgcc taaattgaaa aagatcacga tgaaatctgt accgatggca agtgcaactg
    16261 aagcattgaa ctctaaatta tatgatatga ttttccaaat gccaacagat acttacgata
    16321 cctataaaga aattgctggc tatacaaact taggccgtga acaaacttct tatacttatc
    16381 taggattcaa gttaggtaaa tggaatgcag atacaaagag tgtggaatac aatccagatt
    16441 caaaaatggc tgataaatca ttacgtcaag caatgggcta tgcattagat aataatgcag
    16501 taggtgagcg tttctatgca ggtttgagaa gcaatgcgac ttcattgatt cctccagtat
    16561 tccagtcata ccatgatgat gagatgaaag gattcacaca agatctagac aaagcgaaaa
    16621 aactattaga tgatgctggc tacaaagatg tggatgacga tggtttaaga gaagacaaag
    16681 atggaaataa actaacaatc aactttgctt cgatgtccgg tggggaaaca gcgcaaccat
    16741 tggcagatta ctatatccaa cagtggaaag aaatcggttt ggatgtccaa tacacaacag
    16801 gtcgtctgat cgaattcaat tctttctatg ataaattaga aaatgatgat cctgaaattg
    16861 atgtttacca agctgcttgg aatacaggaa ctgatccaaa tccaagtgga ctatggggac
    16921 caaatgctgc gttcaactac actcgttatg aatcagaaga aaatacaaaa ttgatcaacg
    16981 atatcagttc aagcaaagca tttgatgaag agtaccaaaa agaagcatat caaaaatggc
    17041 aacaatatgc atttgaagaa gcatttgcga ttccaacgtt gttccgtaat gaagtattgc
    17101 cagtcaacga ccgtgtcaaa gactgggatt ggtcttatga tgtaccaaac ccatgggcaa
    17161 atgtttctgt tacagcagat tcacgttcat aatcactaaa aatgaccgaa aaagggcgat
    17221 gtcctttttc ggttttttga tatttttctg tttatttctc taatattcag gtcaaggcgc
    17281 tgatttcatg acttctccaa taaacggtgt tcaaagtcca gtttctcggt attaagtcaa
    17341 atttatgcaa aaatatggaa agccattttc acaaaatttt ttcttcatac tcgaaactaa
    17401 acgaattttt cacaacttct cgtcaatcac taggattatt aaagctttac ttaccttttg
    17461 accgaaccat ttcaaatttg acaaaaaacc agtataataa ggaagtatcg aaaaaatatc
    17521 cataaaagga atgtggaaaa tggacaacca gctgacaaaa gaactaaaag aaaactataa
    17581 catcgttttt cacgatctaa atttgcttga acaagctttt acccattcat cctatgtgaa
    17641 tgagcatcgc aatctgcaat tatcggataa tgagcgactt gaatttttag gagatgccgt
    17701 tttagaactg atggtttctc agtatttatt ccgtttatat cctcatttgc cagaaggaaa
    17761 attgacaaaa acacgagctg cgatcgttcg cgaagacagc ttgtcgaaat ttgccaaaga
    17821 atgtcacttt gatcaatatg ttttgttggg caagggagaa gagaattcag gaggacgtac
    17881 gcgaccagca ttgctttgcg acctattcga agctttttta ggcgcgttat atcttgacca
    17941 gggattcgac acgacccatg cattcatcga aaaagtgatt tttcctaaag tcaaagcagg
    18001 tgctttttca catgagatgg atcataaaac aaaactacaa gaagtcttgc aaaaatcagg
    18061 tgatgtatca atcgaatacc gattgatcaa tgaagaaggt ccagctcatg aacgagtatt
    18121 ttggatcgaa gtttatgtag atgatcaatt gattggcact ggacaaggaa aatcgaaaaa
    18181 attagcagaa caagctgcgg cggaaaatgc attggcagca ctgtaaaagt aagtgaggga
    18241 aagctttgta tttgaaacga attgaaatag ccggttttaa atcatttgca gacaagacag
    18301 tcattgactt tgaaaatagc gtcacagcag tggttggacc aaatggcagt ggaaaaagca
    18361 atattacaga agctatccgc tgggtattag gagaacaatc tgctaaaagt ctgcgaggcg
    18421 gaaaaatgcc ggatatcatc tttgccggtt ccgatacccg taagcagctg aatattgcag
    18481 aagtcacagt gattttagac aatagtgatc attatctgcc attggagtac aatgagatca
    18541 gtgtaacccg ccgttatcga cggactgggg aaagcgagtt tttcatcaat aaacaaagct
    18601 gcagattaaa agatatccaa gaattgttct tagactctgg attaggaaaa gaatcgttct
    18661 caatcatttc tcaaggaaaa gtagaagcga ttttcagtag taaaccggaa gaccgtcgtg
    18721 ggatctttga agaagcagct ggtgtcttga aatacaagca gcggaaaaag aaagcagaac
    18781 agaagctttt tgaaacagaa gacaacctca gccgcgtcca agacatcatc cacgaattgg
    18841 aagaacagtt aactcctttg gccgcccaga gtgaggcagc aaaggagttt ttacggctaa
    18901 aagaaacact gactcaaacc gatgtctctt tgatggttgc cgaaatcaag acagcaaaaa
    18961 aagattggga caacaaacaa gcacagcttg cgaaattcaa tctagaatta ggcaaactat
    19021 ctgagtcgat ccaagaacaa gaatccattt tggcgaagca gcgtaaagaa aatgcgcaag
    19081 ccgatcgtct gatcgaaaaa aatcagcaag tattattaga tctttcggaa aaactgaagc
    19141 agacagaagg acaaaaagat gttttgcagg agcggacaaa gcatacacaa aaaagctcgc
    19201 aagaatatca aacttcatta gcagaagcac aaaaaaaggt taaacatttt gaaaagcttc
    19261 aagaaagctt gttgaaagaa gtagcagaaa aagaaacaga aatccaaaaa gctgaagcaa
    19321 gtctcataaa aacacagcaa gaattagaaa aataccaaaa atcaacgaaa gaattactgg
    19381 cagaactaag agatcaatat gttgatttga tgcaagagca agcagctgta ggaaacgaac
    19441 tgaagtatct agaacgtcaa tatgtacaag aaacagcaaa aagtaagcag acgttagcca
    19501 agcaatcgga agtagaggca tcagtagacc gattgatctt acaaaaagaa gaattgacac
    19561 aaaagcaggc gcagctaaaa tcatctttaa ctgaaacaaa agagaagtta gaaatgatcc
    19621 aacaaaacgg gaaaaagttc caagaagcat tagcaaaaga acagcctaaa atgtatcaat
    19681 taatgaatca agtccagcag ctacgtgctc gtaagaaaag ccttcaagaa atccaagaaa
    19741 actattttgg tttttaccaa ggggttcgat tggtcttgca gcataaacag cagctttcgg
    19801 gtattgtcgg agcagtggct gaactgatcg atgttcctgc agatttcacg ttagctatcg
    19861 aaacagcttt aggtggggca gcccagcatg tcatcgttga aaatgaaaaa gatgctcggc
    19921 aagccatcac gtatttgaaa cagcaaagag gaggaagagc aacattcctt ccactgacga
    19981 caatcaaacc aagacaatta ccggctcata tattgacaca agcagcagct gtagaagggt
    20041 ttatagggat tgccagtgaa caagtcagtt atcccgatca aatccaaact gtggttcaaa
    20101 acctgctagg aacgatcctt ttagccaaag acttaactag tgccaatgca atcgcgcaaa
    20161 cgatccgcta tcaatatcga gtggtttcct tagaaggtga tgtgatgaat gccggaggat
    20221 ccatgacagg tggagcgaat aaacgaggaa accaaggcag tttgtttgta caaaatcaag
    20281 aattgaaaca gttgactagt gaatttgaag aagcagataa acaactccaa gcacaagaaa
    20341 aaaaagttca ggagctgcaa caagaaacag ctcgtttagc cgaagaacaa gaagttttgc
    20401 gtacacgagg cgaacaatta cgttttgaag aacaagaagc gacaaatcag ctgcaaaaca
    20461 tcataaatga acttgaacgt tttgaaaaag aaaagcaaat ctcaactttt gagacacgtg
    20521 aactgcagca gtttatcgaa gattacgaaa agcaaacgaa cgaattgaaa gacaaacaaa
    20581 cagatcttga atcgcaacga caaaaaatcg acgaagaaat caaatcgtta agtcaagaaa
    20641 gcgaccagat ggaagccaga agagcacaag tacaaagtca aaaagcacaa gaacaagcag
    20701 atcttgctgt cttgaaagag cagtttaatc atctgcagat ccagctccgc ggtgcccgag
    20761 tccaaaaagc agaggcaacc gaacgtcaag aagcgataga aaagcagttg gcgacattga
    20821 cagcagactt ttctgatcat gaagtcacag aagaaagttt ggaaaaacaa attaacgaat
    20881 tatctgctca gcgagaaaca ttgaaagccg agttggcaaa agccaaagag cagcgagata
    20941 gaaggcaaaa ggagatcgat aagcttgaag cagtcttggc agagcgtaat cgtgagcaaa
    21001 aagcacgact gtcggaacaa tccaaattag aagtccaaaa agaccgagct gaaatgattt
    21061 tagataatca tttgacctat ctgcagtcag aatatcagct gacatttgag aaagcttccc
    21121 aagactatca agaaacaacc gatatcgaag acagtagaac aaaggtttca tccttaaaag
    21181 aacagattga aaaactggga ccagtaaact taaattcaat cgaacaatac gaacaagtca
    21241 gtgaaagaca tacattctta gcaacacagc gagatgatct gttagcagcg aaaaaccaat
    21301 tgtttgaaac gatggatgag atggatgatg aagtgcggac aagattcaaa gaagtatttg
    21361 aagctatccg tcaagaattc aaaattgtct ttccaaatat gtttggcggc ggtaaagctg
    21421 aattagtact gacagaacca actgatttgc tgaaaacagg tattgagatt gaagtacaac
    21481 ctcctggcaa aaaactgcaa agtttaagtt tgctttctgg aggagaacga gcacttacag
    21541 ccatcgcgtt gctcttttct attatccgtg tctgtcctgt tccgttttgt atcttagatg
    21601 aggtagaagc agcgttagat gaagccaacg tgaaacgttt tggtcgttac ttgagcgatt
    21661 tccaagatga cacacaattt atcgtagtca cacaccgcaa aggaacgatg gtcgcagcag
    21721 atgtactgta cggagtgacg atgcaggaat ccggtgtatc aaaaatcgtt tctgtacgga
    21781 tggaagatat caatgaagag gcgaaaatca acgaaacgaa agtgtaggaa cgaaaatgat
    21841 caaattagta gcaatcgatt tggacggtac attattaaat gaacaaaagc tgatttcaga
    21901 tgagaataaa caagcattag cacaagcaaa acagcaaggg gtcaagatcg ttctatgtac
    21961 aggacgcccc ttagctgcga tggtgcatta tctgcaagaa ttaggactgg tagatgaagg
    22021 tgactacagc atcactttca acggcggttt ggtacaaaaa aatgatactg gtgaaatcat
    22081 ggaaaagaaa gtgatggaag tatcagatat ccaacgctta tacaaactgg ctcagcaatt
    22141 ggatctgcca ctagatgttt tgtctgacaa tgtggtttta cagctgccta gtgccccaag
    22201 gaagcaatcc ttgtacaatg tgttgaataa gctgctgcaa ttccaaccag cggatataag
    22261 cgatattaca gatgaatttg tgcttaataa ggcagtaatc gcttatgacc aagaagaatt
    22321 agatcctaaa atcaaagaaa taccagcaga attccatgaa atgtatgaga tcatcaaaac
    22381 gagaagtatg cttttggaat ttatgccaaa aggtgttaca aaagcttatg gtatttcttt
    22441 attggcaaaa gatcttggtt tgaaacaaga agaaatcatg gcaatcggtg atgaagaaaa
    22501 tgatttaccg atgatccaat acgctggact aggcgtagcg atggagaatg ctgtgccttt
    22561 cgtcaaagaa gcagcagatt tcgtgacagc cagcaatgaa gaaaatggcg tagccaaagt
    22621 gatccaaaaa tttgtattag agtagggggg agtcaaatgg gattttttga taaaatcaag
    22681 cgtgctttta ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag
    22741 ggtttagaaa agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt
    22801 cgaacagtcg atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta
    22861 ggttttgaaa cagcgatacg aatcaccgaa gcattacgtc aagaagtgaa actcagaaat
    22921 gcgaaaaaat cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa
    22981 gcggaaggaa tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt
    23041 ttgtttgtcg gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag
    23101 ttcaaaaacg aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca
    23161 atcgaccagt tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca
    23221 ggcggcgatc cagcagctgt cgtattcgat gctttggaaa gagcaaagat agagcaggca
    23281 gatgttttac tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa
    23341 ttagaaaaaa taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt
    23401 cttgtactag atgcaacaac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa
    23461 acaacagatg tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc
    23521 gtacttgcta tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga
    23581 atcgatgact tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta
    23641 ctaaaagaag aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt
    23701 gtttgaatga ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt
    23761 gtaagcggtt ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc
    23821 atatgaattg gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg
    23881 cgccctttta tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt
    23941 gcaggaaatc tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc
    24001 tatatccaaa cagattttag cgaaacacaa acagagatga tcactcctgt taccgattct
    24061 attcccgaat tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg
    24121 aaagaagaga tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagcg
    24181 atcattattg caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa
    24241 gaatacggga agcgaaaaca gatggtaagc ggtatccatt tcaattttga atttggcgat
    24301 gagttgctaa gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa
    24361 acagaacttt atttgaaaac ggcgagaaac tttatgcgct atcgctggat gatcacttat
    24421 ttattcggtg cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa
    24481 gaacctgttc gcagtattcg taacagtgcg ctgggctata cgaatcatcc aaatgtgaaa
    24541 gtttcctatg cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg
    24601 aaactttcgg aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc
    24661 gcagatttag caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct
    24721 tatgcaagat tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac
    24781 atgttatgga cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga
    24841 aataacaaag tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga
    24901 gaaattcttg aagggatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat
    24961 ttagtagagg aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc
    25021 tatcaagaag cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat
    25081 tataaagaaa gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct
    25141 actcagatat ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag
    25201 tcggatcaat ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac
    25261 atgacaagta aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa
    25321 aaagtcttaa aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa
    25381 gaagcggtaa aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca
    25441 accaattacg ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa
    25501 gcaggtttag ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg
    25561 ggaacggaat atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc
    25621 cctgctaatg ttattggtga cggtatccga acagtgaaag aacttgttga agaaaaaaat
    25681 agtgaccctt tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa
    25741 ttagaacaat tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat
    25801 caaattgttt atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg
    25861 actgatgaat tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga
    25921 gctaagatta gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat
    25981 aaaaaagcat atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat
    26041 ccctttgcag gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa
    26101 gtattttaaa agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag
    26161 cttttttgca tgtaaaaatt tatgtgtttt ttcgttttaa aataaacgat cccagaagct
    26221 ttttttcttt tttggttttt ctttttcaac tggcgaagag ttttcttctg ggtatttttt
    26281 ttcgatatca atcacaggtt catttactgc ttcttttgct gttaagagca atccgaattc
    26341 ctcaggattt gcagtgacgt aatcgttgac tacagtaaat cggctattgt ttttcgttgc
    26401 tagctggata tattggtttt ggacagaaag gggtagagcc ccattcaaga gtatggaagc
    26461 atctggatgt tttttgagtt cttttaaaaa gttttctttg ttttttggtt ggatcatttc
    26521 agcaacagtc atactaagat agcagcgttc cctgaatgtc cctaaatatt tatgctgttc
    26581 ttcagggttc actaaaggtg taccataaag tcccttatcc aaatggcttt ttacatcatc
    26641 ttgtgccata aaaaataccc tccttcttct ctttgtcttt cttacgatta tagcatattc
    26701 ccaataatac ctaaaacata tgaaaaagcg gttgcttttg ttcaacgatt cacttaaaat
    26761 aagaatgaaa atcattctca attagaaagg tggattggat gcgctacgga cgactagtag
    26821 cattattagt tattttaagt agtatttcat tgttcatcgg agtatcgaat gtacaattgt
    26881 cagaactgct ccagttagat gggcaaagct tacaaatctt gctagtgact cgtttgccac
    26941 gaacgatttg tttgatcctt gtgggagcga ccagtagtat ttgtgggttg atcatgcaac
    27001 atttgaccca aaataaattt gtttcaccaa cgactgcggg cacgatggat agtgcgcgtt
    27061 taggtatttt agtggctatg attttcctac ctggtgcatc cttgattgtc cgatccttga
    27121 cagcattttg ctttgctttt gcaggaacat tgcttttttt atcccttacg cgattgttcc
    27181 cccagaaaaa tcaattgatg cttccgttga ttggagtcat gttagggaat atcattggtt
    27241 cagttgctac tttttttgct tatcaattcc agttggtaca aaatatgact tcatggcttc
    27301 aaggaaattt tgcgactgtg atacgaggaa attatgagtt attgtatgct gttgttccat
    27361 tgttgctctt ggtttggcta tttgcttacc gatttacgat cattggtctg ggcgaatcgt
    27421 ttgcagtaaa tttgggaata gattaccaga agatgcagtt tttaggcatt ggcttagtat
    27481 cattggctag tgcagtcatg ttgatcatgg taggaagcat tccttttatc ggagtgatca
    27541 ttccgaatct tgtctctaga atatatggag atcaagtgca caaaacaatt ggcgtaactg
    27601 ctttagcagg tagtttgttc ttagtatttt gcgatcttgt cgctcgcgtg ttgatttttc
    27661 cttatgaaat accagtcagt gtgatcgttg gaatcatcgg tggggctatc ttcctttatc
    27721 ttctgataag ggggcgacga tatgcttaaa aaaaagccat ctatttttat tggtatgctt
    27781 gttcttgttt tatttgttac aggaatgtac ttgacgatcc atacatatgg aaactggcat
    27841 tttgcatggg cattgaggag taagaaaatt tttgcattta tccttgtctc gctagctgtc
    27901 agcagtgcca caatcagctt tcagacaatt acgcataacc agtttttgac tccttcagta
    27961 ttgggacttg agtcgttata tgtccttgtc caaacggtcc ttttcttctt tatcggagga
    28021 gtgacgatgc ttcagcagac aggtacagtt tttttcttat tgaacgttgg gctgatggtc
    28081 gggctgagtc tattgttatt tttctttctt ttgaaaaaaa tgagagcgaa tcttttttta
    28141 ttactgatgt cagggatgat tttaggtacg ctttttggta gtgtcagtac ctttttacaa
    28201 gtcatcatgg atccgaatga atatgatttg cttcaaggca gattatttgc aagctttggc
    28261 aatatccaga gtcagtatct tttgcctgct tctttgatca ttatcggctg ctggtacctg
    28321 ttatttcgat atcgacatgt gctagacgtt ttccatctcg gaaatgaaca agcagtaaac
    28381 ctcgggatag acacagatcg ttttgaattt ttgattttat gttgtgtcag tcttttgaca
    28441 gggacatcaa cggctcttgt gggtccagtt acttttttag ggtttattgt cgcaaatatc
    28501 agttatcgtg tcttttctac ttatcgtcat ggtcagctat ttatcggaag ctttctggta
    28561 gctatgctgt ttctgacagg aggacagctc gcagtagagc agattttcgg ctggaataca
    28621 acagtgagtg tcgtcgttga atttgtcgga ggaatttatt ttgtcacaaa attgattcgc
    28681 gaaaggaaga gtatcctatg attgatttac aaaaggtcaa taaactgtac catggaaaat
    28741 cagtggttaa agatgtacag ttaacgattc ctgaaaagaa gttgactgct tttatcggtc
    28801 caaatggtgc tggaaagtca acggtgttgt cgatgatcag tcgtttgata ccaaaagata
    28861 caggtgaaat ctatttagac cataacgaag taaaggcttg gaaatccaat gaattagcca
    28921 aaaaattatc gatcctacga caatcgaatg aaataaatgt ccaaatcacc gtgaaagaat
    28981 tagtagaatt tggacgattt ccttatacga aaggaaaatt gacgacaaaa gacgaggaaa
    29041 tcgttcagga atcactggaa caccttgggt tagaagaaat agcggatcaa tcgattgaca
    29101 ctctttcagg cggtcaactc caacgtgcgt atattgcaat ggtgctagcg caagacacgg
    29161 attatatttt actggatgaa cctttgaaca atctagatat gaattttgcg gttcaaatca
    29221 tgcagatctt aaaaaaatta gtacaagaaa tcgggaaaac aattgttatt gttcttcatg
    29281 atatcaactt tgctgcaagt tatgcagacg aaattattgc aatgaaagat gggcagttat
    29341 tcacgcaagg aacgacccaa gaaatgataa aaaaagatgt actggacgaa ttgtacgaaa
    29401 tggacatacg aatttgtgag attgaaggtc gcagattctg tctttatttc taaaactaac
    29461 attaggagag aagagatgaa aaaattagta gtaggtatct tgattctagc atcatttggt
    29521 ttagcagcat gtggaaatag tgacaacacc agtcaggcag atacaaagaa aagcagtacg
    29581 caagctgaaa cggcattaac gataaaagac agtaatggcg atcaaatcga ggtgccaaag
    29641 aatcctgaaa aagtcgtcgt attcgacaat ggttctttag atacgatgga tgctcttggt
    29701 gttggagata aggtagttgg agcagctaca agcagccttc ctgagtatct ttcatcctat
    29761 aaaaaagtgg aatcagcagg tgggatcaaa gaaccagact tagaaaaaat caatcagctt
    29821 cagccagacc tgatcatcat ttctggccgg caaagagatt ttcaaaaaga tctaagtcaa
    29881 attgctccga ccattttcct ttctttagat gcgaaaaatc catgggaatc tttccagcaa
    29941 aatgtcactg ctttagggga gatctttggt aagcaagaag aagctaaaac tcaattagag
    30001 gaattatcta gtgcaatcga tcagacgaaa aagaaagcag aagctactga caaaaaagca
    30061 ctagtcacac ttgtgaatga agggcaatta tcagcctacg gatctggttc tagattcggt
    30121 ttcatccatg atttatttgg ctttgaacaa gcagatgatc aaatcgaagc atccactcac
    30181 ggtcaaagtg tttcttatga atatgtgctg gaaaaaaatc cggatatctt atttgtagta
    30241 gacagaacaa aagctattgg tggagacgac agcaaagatg atatttctgc caatgaactg
    30301 gtagcacaaa cgaatgctgg taaaaatcag cagatcatct cccttgaacc agatgtctgg
    30361 tatttaagcg ggggcggttt agaatcaatg aaactgatga tcgaagatgt gaatcaagca
    30421 tttaaatagc ataaataaca gaggattaaa ctctcatact atgtaacaat aatgtttctt
    30481 acattcttaa atagcaagag cctatcctca aagaagttat caaaaaccat ccgtaaaatc
    30541 gtattgatga ttttacggat ggtttttatt tatttgggaa atcttttttc aacgtgaatt
    30601 cattctctac atcatacggg ttgattctag aatggggagt ggcaaaaaat agtgtgatcc
    30661 attttttcag catcaagatc gcctcctttg atttgaatca aaggtacaaa gcgaaactta
    30721 gataaaactt aaatttagat atttttattt tgtaccgatc tcttctttaa acgatgttcc
    30781 atctgctgct tctcggcaat aagtccacta gactagaaaa tatgagaagc tattttctgt
    30841 ccagcgttct tattactcaa agcaaatcgc agttgtcaca atctcttcta aacgatgttc
    30901 catctgctgc ttctcggcaa taagtccact agactagaaa atatgagaag ctattttctg
    30961 tccagcgttc ttattactta aagcaaatcg tagttgtcac aatctcttct aaacgatgtt
    31021 ccatcagctg accttcggta ttagctcaaa gcactgattt cacaacctct atttaggcat
    31081 gtgtctcagc aagcggttga tagttgatga tctgcatttt tcgataaaaa tcggcgggaa
    31141 gcttactcca ccgttttaat aattgataga aataactctc ttctgctttg attttatact
    31201 cgcttaatat caatccaaaa ctattcgttt tttttgaagc taatttttta taaatatcca
    31261 atgctttggt tttatcttca ttgacaaaga gtgtttttac ttcaatataa gaaccctcga
    31321 tattttgtgt ttcttcgata aaatagatgt tttttatata ttccttcata gtgattcctc
    31381 caaagaagtc tttttatttg ataagtttac tctaccgaag agttgttaat aatccgtgaa
    31441 caaaaagtaa ttctttttcc aatagttgtt aaaggttttt tattttttat agtatttttt
    31501 gttatttaag aaaggaataa ggttaaacaa ataacaggtt gctttttggg tctttttccg
    31561 ttcaagaaac tgaacgaaga aaagggaaag ttgtctcttt tgtgataaga gaaatcataa
    31621 taaacgaatg acttactttc caataaattg agaacagtca gaaccagaca aattagttgc
    31681 atctgatgaa taaagtgatt attctatgac atgaggtgat acagatgaaa ttagaagtga
    31741 caaaagaagc acaagaagta ttgaagaata aattagaagc agacgatcaa cttttattag
    31801 atatcgaaaa cggtgacggt ccatttgcag aaagccaagt cagttgccaa ttggacacgg
    31861 catttcgatt gatcattgtc aaaaaagata cacaaacaga tctaagtctt tattctgtag
    31921 tggctgatac accagttgga ccaatcaaaa tcaaagacag tgcaatactt tacatggatg
    31981 atccatccac acttgcttta gaaccaactt ataatagttt acaattaaaa ggaccaagcg
    32041 gcttgagaaa aggcaacttg caagttgttc gaaaagacta gagggataaa atgggaaaat
    32101 tggcgatcat cagcgaccta catgtggaca tcaagaaatt cggcgaatca gagctgatgc
    32161 agctttggca agttttgcaa aagcacaaga tcactcggct tcatctagcc ggagatacgg
    32221 ctaataaagt agaccgttgc ctagctgttg tcgaattttt cgtcaataaa ggattatcta
    32281 cgacatttca tttcggcaat cacgagttgg cagatgtgcc caatgaacag atgatggacc
    32341 attttccaga cccacatttt ttaaatgaac ggtatattgc tttaaatgag cagaccgttc
    32401 ttttagggat gaatggctgg tacgactatc agttttccga attgaaagat tacgaggcaa
    32461 tcgtacgaat gaagcgtttg tattggtatg atcggttcat ccagcgtggg aaaacagatc
    32521 tcgaggtcaa tgcagaaatg cttgcagctg caaaaaattt gctagaccag ctggaagcaa
    32581 aaaaacttga ggtgatttta tccactcatt tcgttcctaa acgggcattt atcatgtatc
    32641 aatctgctcc ttatgagcgc tggaacaaat taaatgcatt tttaggatca gaatcattcg
    32701 gagaattact tgaccgttat tcaaatatca aacaagtagt ttttggccat acgcatcgtc
    32761 gctttgaaga taaatccatc ggtcacacga tttacagctg cagacctttt ggttattatt
    32821 atgaatggca gctgacccga gattttgtct tgaaagaaca gctgttagat agctatgaac
    32881 ccatgaaatt acgtgtcctc ttgcgtgccc atcatccggc ctttcaagcc tatcaaacac
    32941 aacatttgca agaggaattt gaacaggcga tgacaatcgt tccgtattga gacagcatta
    33001 gtaatgtgta aaagaaaata ccttgtaaaa cataaggata agtaagagca gtagatccat
    33061 ttatcgtgga tatgctgctt tttttgtgga aaataatcag cgaatgttta tttgatcgga
    33121 tgatttgtaa ctcgcagagt actagatgca agcgtttatt ctttttttga acgataatca
    33181 caaaaaaaaa tataatatac attttttat
//
LOCUS       DACEUF010000024        32292 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.025, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000024 DACEUF010000000
VERSION     DACEUF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.025"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..226
                     /locus_tag="IXG20_001089"
     CDS             <1..226
                     /locus_tag="IXG20_001089"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ1609301.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="IXG20_001090"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="IXG20_001090"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="IXG20_001091"
     CDS             533..1336
                     /locus_tag="IXG20_001091"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ1609302.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(1585..2649)
                     /locus_tag="IXG20_001092"
     CDS             complement(1585..2649)
                     /locus_tag="IXG20_001092"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ1609303.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(2646..3731)
                     /locus_tag="IXG20_001093"
     CDS             complement(2646..3731)
                     /locus_tag="IXG20_001093"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ1609304.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="IXG20_001094"
     CDS             complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="IXG20_001094"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ1609305.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="IXG20_001095"
     CDS             complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="IXG20_001095"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ1609306.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5980..6633
                     /locus_tag="IXG20_001096"
     CDS             5980..6633
                     /locus_tag="IXG20_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1609307.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            6718..7623
                     /locus_tag="IXG20_001097"
     CDS             6718..7623
                     /locus_tag="IXG20_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1609308.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(7625..8257)
                     /locus_tag="IXG20_001098"
     CDS             complement(7625..8257)
                     /locus_tag="IXG20_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1609309.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            8577..9101
                     /gene="infC"
                     /locus_tag="IXG20_001099"
     CDS             8577..9101
                     /gene="infC"
                     /locus_tag="IXG20_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ1609310.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            9173..9373
                     /gene="rpmI"
                     /locus_tag="IXG20_001100"
     CDS             9173..9373
                     /gene="rpmI"
                     /locus_tag="IXG20_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ1609311.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            9426..9785
                     /gene="rplT"
                     /locus_tag="IXG20_001101"
     CDS             9426..9785
                     /gene="rplT"
                     /locus_tag="IXG20_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ1609312.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            10137..11375
                     /locus_tag="IXG20_001102"
     CDS             10137..11375
                     /locus_tag="IXG20_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ1609313.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            11423..13552
                     /locus_tag="IXG20_001103"
     CDS             11423..13552
                     /locus_tag="IXG20_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ1609314.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            13527..14216
                     /locus_tag="IXG20_001104"
     CDS             13527..14216
                     /locus_tag="IXG20_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609315.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(14624..15310)
                     /locus_tag="IXG20_001105"
     CDS             complement(14624..15310)
                     /locus_tag="IXG20_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1609316.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(15446..15640)
                     /locus_tag="IXG20_001106"
     CDS             complement(15446..15640)
                     /locus_tag="IXG20_001106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609317.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(15690..16130)
                     /locus_tag="IXG20_001107"
     CDS             complement(15690..16130)
                     /locus_tag="IXG20_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ1609318.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(16134..16979)
                     /locus_tag="IXG20_001108"
     CDS             complement(16134..16979)
                     /locus_tag="IXG20_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ1609319.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            17128..18546
                     /gene="pepV"
                     /locus_tag="IXG20_001109"
     CDS             17128..18546
                     /gene="pepV"
                     /locus_tag="IXG20_001109"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ1609320.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(18622..19824)
                     /locus_tag="IXG20_001110"
     CDS             complement(18622..19824)
                     /locus_tag="IXG20_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609321.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(19811..21598)
                     /locus_tag="IXG20_001111"
     CDS             complement(19811..21598)
                     /locus_tag="IXG20_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609322.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(21611..22564)
                     /locus_tag="IXG20_001112"
     CDS             complement(21611..22564)
                     /locus_tag="IXG20_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1609323.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(22749..23099)
                     /locus_tag="IXG20_001113"
     CDS             complement(22749..23099)
                     /locus_tag="IXG20_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1609324.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            23299..24378
                     /gene="pepA"
                     /locus_tag="IXG20_001114"
     CDS             23299..24378
                     /gene="pepA"
                     /locus_tag="IXG20_001114"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ1609325.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            24521..24841
                     /locus_tag="IXG20_001115"
     CDS             24521..24841
                     /locus_tag="IXG20_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1609326.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            24863..25327
                     /locus_tag="IXG20_001116"
     CDS             24863..25327
                     /locus_tag="IXG20_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1609327.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            25523..26128
                     /locus_tag="IXG20_001117"
     CDS             25523..26128
                     /locus_tag="IXG20_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ1609328.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(26167..27456)
                     /locus_tag="IXG20_001118"
     CDS             complement(26167..27456)
                     /locus_tag="IXG20_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1609329.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            27884..28363
                     /gene="rlmH"
                     /locus_tag="IXG20_001119"
     CDS             27884..28363
                     /gene="rlmH"
                     /locus_tag="IXG20_001119"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ1609330.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            28716..29030
                     /locus_tag="IXG20_001120"
     CDS             28716..29030
                     /locus_tag="IXG20_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1609331.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            29046..29432
                     /locus_tag="IXG20_001121"
     CDS             29046..29432
                     /locus_tag="IXG20_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1609332.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            29461..30846
                     /locus_tag="IXG20_001122"
     CDS             29461..30846
                     /locus_tag="IXG20_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1609333.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            30849..31002
                     /locus_tag="IXG20_001123"
                     /pseudo
     CDS             30849..31002
                     /locus_tag="IXG20_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            31025..32230
                     /locus_tag="IXG20_001124"
     CDS             31025..32230
                     /locus_tag="IXG20_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1609334.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT        10233 a         6043 c         6617 g         9399 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggacagaagc gtttaactcc aagaaataag aaggaattca cgaaaattgc ttttcaaatt
     1501 tttgtgaatt tcagcttatt tccgaaggag ttgcttttgc ttccgccgtt tatacgtgtt
     1561 tagagcgtgg gataaaagtg ttctttactt ttgtcccacg ctcttttctg tgcgtcttcc
     1621 atacttcact aagtcgatcc ctcttgcttc gcaccaatcc ttcactggtc cggaaaagat
     1681 cagtgattgt tggtgtaggg ccgcggtatt cgttgcgccg accatagtat aaagcagacg
     1741 aagctcttct tgccattgct tcatcagtat aatagtttct tctacgccgt gattcatcag
     1801 atgcgtcagt acggttcctg aagtacccac tgcatttgcg ccgagacaaa gggctttaaa
     1861 tatgtcatag gcattgcgga tacctccgga ggctaggatt tccatagaag tgtctgcttc
     1921 atttgcttca agcaatgaag agactgtcga ttgcccccag tcagctaaat aactcagttc
     1981 ccgcttggag cgacgggcgt tttcgatctg ggtgaagctt gttccgcttc gaccgctgat
     2041 atcaactgta tgaactccta aagcagctag atcattgagc gtttctcttg tcatgccaaa
     2101 accgacttct tttacgatga gggggacgtc aatggctgtt tgagtctctt gaataagtac
     2161 tttccaattg gtaaaatcac ggtctccctc aggcataacc aactcttgag gtgcattcaa
     2221 atgaatctgt aaggcatctg cgtgaaacaa tcggatagct tcttgggctc tttcgacaga
     2281 agtccctgca ccgatgttgg caatgatttt cccgtgagga tattcttgtc gcataatcgt
     2341 ataagtatcg gctaaagaag ggtctttcaa ggcagcgctg actgaaccag tggcaatcat
     2401 caaatctgct tctcttgcga cgattgcaag atcttggttg attttttttg ttttttcgct
     2461 tccgccagtc atcgcattga tataaaatgg gctgcttagt gtaaacccaa ctgcctgtgt
     2521 acttagatcg acatcagaaa ctgctatttg cggcaaaggg ttatggatga tccgtacgaa
     2581 gtcaaattcg tttttttgtt tatcatgaaa ggctttagct agtgaaacat gttcgtcttt
     2641 tcgattcatc ggttttcctt tctttgatcg ctatagacat gtaacggcag tggagtgatt
     2701 tctgcttttt cccatgcact cattaaagga aggatgccag atttctggtc aacaattacg
     2761 attccgcaat cgccaccacc tgcaccagaa gattttgcgg ctccttcata ctgttcagct
     2821 aaattacaca atttgttcaa agcaggcgtt tcgatgacga ccccagtgat tgcagataaa
     2881 tcatgcagta attgtcggtt ttttcgaatc atctgttgga tcaacgttac attattttct
     2941 ttaaaccctt tgatcatttc gttgacacat tctgtactgt tttttaagaa aagctgataa
     3001 gccaccattt tgtcttctct cgaacggtga acttgatcga ccaaatcaga agtagaggca
     3061 gggctacccg tccaaccaat caataaacgt aaatcttcag gagcaatcaa tggctcaatg
     3121 gatagacctg gccaatccag ggcaagtaac tcactgatag aatgctgagt ttcttgttct
     3181 tggagccaag gatggtcgaa ggttgagaaa gcgatccagc caccataaca gctagcagcg
     3241 atgtcgccgc aggaaccatt atcttgaact gctaaattgg ctagtgctgc aatcttgaaa
     3301 atctccaatt gagataaatt caacgcgtaa aaaacattca aggctttgac cgttgcaacg
     3361 gtcacggcac cgctagaacc caatccatat ttccggccat ttgaactgtc taactcactt
     3421 gttactttca gatcataaaa ggataaaaga atgttttttt cttgtgcata cttttcagtc
     3481 aaacgaatag cagcaaggat ataatgaaaa ggattttccc gaatatctaa aactaattct
     3541 ccgttgcgtc ttgtccaacg tacaggcatc ccactatatt gtgcagattg gatacttccg
     3601 acttttcgtg cggattctac agtgactgtt acgaattgat cgactgcagc gataactgct
     3661 ggatgtcctg tttcaacaac tgcatattct ccggcaatat agagtttgcc tggtgcagat
     3721 acttcaatca taaaaatctc cctttattca ataatcgcaa ttcctggacc agcatgggca
     3781 gtaattactt gttctttgct aaataatttg gtaaatgttt cttgtacctc agataaatgg
     3841 ctgttttcca ctaaaacttt gacattaggg ccagcatcca tcgtaaagta gcatggaatg
     3901 cctttttttc tcaattgccg caccgcatcc atcgctttta agctgtcagg agaccagtaa
     3961 gtgaatggag gctgggcagc taaagtcgtt ccatgcattt ttagtccgtt tctttccatc
     4021 gtttctccga gaagttggaa atcttttgtt ttgattgctt gtttcaattg atatagatcc
     4081 ccttcaacgg aatctaacca gccttgataa aaactggatg tttcgactgt tcgacgcatc
     4141 ccatttctgc tggacacttc ttttttctgg tcgttgatca aaacgaaaac cattgctaaa
     4201 tcgtcttcga aagaatcgga agggactggc ttagcgtaag aacttaagtc gtcatggcct
     4261 ttttcccatt caacgaaacc accgaaaata cttcggcaag ctgaaccaga cccgcgtcga
     4321 gcaaatcttg aaagagagag atcgtctaat ccaagcttaa gagcagtatt gcaagctcct
     4381 gctaaagcag ctagcccgct ggcagaggag gctaatccag ctgcggtagg aacgaaattc
     4441 tgactgatta ctgaagcttt tagtgagagg ccggcttctt tacgaaaaag gtcaagaaac
     4501 tgactgactt tttttgttgc tttttcgtct tgtaaggtgc catctagata aaattcatct
     4561 accatatagc tatcagaaaa gatgacttct gtctctgtat aaaatgcatc caatgttaac
     4621 gaaaggctgt tgttcattgg aaggatgagt tcttcatttt tcttacccca atatttgatt
     4681 agagcaatgt tggtatatgc gcgtgctttg cctttaaaca taggtatgta ctcctaatcc
     4741 ttgtatccaa gtttcagcag cacccgcatc ttcaagtgct tgactgattt cttgggcttt
     4801 tgtttttgtt tgtgctaatg caatcataca gccaccgcgt ccaccgccag ttaatttggc
     4861 acccaatgcc ccggtatctt ttgctgtttg gatcaataga ttaagaaaat cattgctgat
     4921 tgtcagcttt tccagaagtg actgtgattc atccattgtc tgtgccaacg tttctggtga
     4981 attctcaatg attgcttgtt ttgcttgctt cgtcaagtat cctaattttt ggatcatttg
     5041 tccagtttca tgaggctggg tttcaaagag gtgagcgaca tctttgacgg cttctcttgt
     5101 ttgtcctttg attcctgtat cagcgacaat caaaaaagca tcgatgttca aagaaaagta
     5161 gtcaaaagga tgcccttttg taaaataaat cggctgcaag ctgctagtag cggctgcatc
     5221 gattccacta ggattaccgt gggcgatttt ttccgaaagc tggacatttt ctaatagtat
     5281 ttcacgagac aaaggaaatg ctaagtaatc ataaaaagca cgagtgactg ctgtggcgac
     5341 tgcagcgctt gatcccattc cacgttcagc aggaatcgtg ctttcaatgg tcagttgcaa
     5401 gtttttttgg atgtcatgct gtttttttaa gtgaatgaat aattttttga tattttttaa
     5461 tgcatgagga gcttcggcta aatttcctga atagtaggaa gaaaccagtt gatcgtccat
     5521 tgcatccagc tcttcaagga atgcggtgac ttttgttgca tagaaaggaa aggcaatcgc
     5581 tggttctcca taaacaaccg cgtgctcgcc catcaatatg atctttccgc ttgattctcc
     5641 ttggccatag tttgccataa tctaactcct tctcacgtaa aagtgatttt taagcaaaaa
     5701 ataaagcgcc caaaacgagt tgcgctgctt cacgaatttt tttgtccact aagggtctca
     5761 aaaaacattc ctcctttatt ttactaaaac catctcagaa gtaaaactaa gaatacgttt
     5821 tctaaagaaa aaacactttt ttcttaagaa ttttttgact ttttttcttc tttgactaga
     5881 aaaaatacaa ttgacactac tagatattgg gggtatattt aaaaaaggtt ggagttaaac
     5941 acaatattta gtgatgaagt gagctggagg taaatgagcg tgatcgtttt agcaggtaca
     6001 attggtgccg gaaaatcaag tttaacagaa atgatggctg aacactttga tagtcaggca
     6061 ttttatgaat cgattgatga taatgaagtc ttgcccttat tttatgcaaa ccctgaacag
     6121 tatgcttttt tgctgcaaat ttattttttg aataaacgtt ttgcaagcat caaacaagca
     6181 atgaaagatg acaataatgt attggaccgt tcgatttatg aagattcact gcttttccat
     6241 ctaaatgcag atctaggaag agcaacagaa acagaagttc gtgtgtatga tgaactgctg
     6301 gaaaacatga tggaagaact tccctatgct gcccataaaa aacatccgga tttattagtt
     6361 catattcgtg tatcttttga tacgatgtta gagcgtatcg aaaaacgtgg tcgttcgtat
     6421 gaacaattgt cctttgaccc aagtctatac gattattaca aagaattgaa cagacgttat
     6481 gatcaatggt atgaagaata caaagaaagt ccaaaaatcc aaatcgatgg agaccgctat
     6541 aattttgtgg aagatcctca agcaaaagaa gaagtcttga aaatgatcga agaaaaatta
     6601 gcagagattc gccaaaccaa agtatccatt tgatttacag ttgctcaaat aaagggtaaa
     6661 cctttatttg agcgactttt ttctttatac tggaaacaaa tgtcaggagg atagagtatg
     6721 cgggaaaaac actattttaa tcaagaccgt aatttttgga tcaatgtaca gcaaaacgat
     6781 gtgctagctg ttaaaaactt aaaagaagaa tatggcatta gtgacgagat gctgacttat
     6841 tctctagata aaaatgaacg ggcgcgcgta gagtacgatt cttttgatga agcgttactg
     6901 cttgtatcaa atgttccgca ccagcaaaaa gtcgataatc attatgaaac tagtcctatc
     6961 gcttttattt taaaagaaga cggtttgttt acatttacca cgccgaatac cgagtacgtg
     7021 attcgactaa ttcgatcgct tttggaacgg atgccagaca tgagtgtata cagtttattg
     7081 tttcgtacat tgtttttgat ttctgattcc ttttttcctt taatcgagga agtgaatagt
     7141 gaacgtcagc ggcttaatct gaaattaaga gaaaaaacaa cgaacaagaa tctgctgcaa
     7201 ctttcagatt tagagatcgg tttggtttac ttagttacag gaacaaaaca aaatgtagta
     7261 cttttagaac aaatcaaggc actggctatt tatcgcaagt tgtcagaaaa ggaaaaagaa
     7321 gagttggacg atgcactgat cgaagcacga caagcagttg aaatgacgaa cttagcttcg
     7381 cagattttag accagctctc tggcacttat aataatttgc tgaataacaa cttgaatgac
     7441 acgatgaaat ttttgacggt ctggtcgctg atcttgaccg ttccaactat tgtgacagga
     7501 ttcttcggaa tgaatctcca gttacccttt acccattccg ttttcggatg ggaaatcgcc
     7561 ttgatcatca gtttggtctt atctatttgg atgcttattg cactgtggcg gcggattcgt
     7621 tgatctagtc tttgatctgc aaaccaaggg cttgggcgat cacaagtgct tgctcttgat
     7681 tgacgattag tcccgccaaa gaatcttgag ataaagcgat tgttgtgaag aaattagtcg
     7741 taaagtctaa tcccttcaga gaagttcgaa accagttcga accggttaac tgattgtcag
     7801 acaaaaaaag gtttttccaa gtgacctcat aaaattcgct gtcttctaat tgacaatgat
     7861 cgaaggagac agctttcaag tttgtactac taaaggaagc catcgaagca atacaatcag
     7921 tgaaagtaca gtctcgaaga taactttcgg caaaattcgt acctgttaat ttacattgat
     7981 gaaacacgac ttggtggaaa ctgccaccaa tccactccag attggaaaaa tcacattttt
     8041 caaaaatgac attactgcat tcaaaatgac gtaaacctgt tttttgcatc gtaagttttt
     8101 ggatatgact ctctcttaat accatatttt tggcatcgag atagctttgg ttacctccgt
     8161 tgatcagaag accagagaag atctcctcat cttctaaata aaactctccc tttgtaagaa
     8221 gaggaagaga aggtggaata ggttcgattt ttttcatgtg ggttcacctc gtttttcttt
     8281 agtatagcaa agaagcggga atcactgcta gaaagcctgc taatctaatc attttcagaa
     8341 aaggaagaca aactgtatga aaaacattga caaaaagatt agtttttgct atactataat
     8401 ggttgagaaa taaaagcaga agcacccgct tctcgcctta gttgataatg tcactgggct
     8461 agatagtttt cactggttct attaggtgaa gtacgcgcgg gttctgtaag agaactcgtt
     8521 tttttattgc cgtttattcg gtaagctttt ctttctctca taatatttgg aggtgaatga
     8581 ccatagcaaa ggatatgatg gttaacgacg gcattcgtgc acgagagtta cgattgatcg
     8641 gttcagatgg tgagcaatta ggagttaaga caaaagcaga agcattgaat attgcagaac
     8701 aagcaaactt ggatcttgtg ttagttgccc ctggtgcgaa accaccagtt gcgcgaatca
     8761 tggactacgg taaataccgt ttcgaacaac aaaagaaaga ccgcgaagcg cgtaaaaaac
     8821 aaaaagtgat caatgtaaaa gaagttcgct taagtcctac gatcgacttg aatgacttca
     8881 atacaaaact tcgtaatgca cgtaagttct tggaaaaggg cgataaagtg aaagcttcta
     8941 tccgtttcaa aggccgtgcc attacccata aagaaattgg tcaaaaagtc ttgaatcgct
     9001 tagctgaaga aactgcggat attgcaacag tggaacaaaa agcgaaaatg gacggacgca
     9061 gcatgttctt gacgctggca ccgaaaaacg acagtaagta atttgaatga ctaactggct
     9121 gttggacagc cgatagtcgg ataacgaaag attttgagga ggaaatatag tcatgccaaa
     9181 acaaaaaaca caccgcggat cagcaaaacg tttcaaacgt actggtaaag gcggattgaa
     9241 acgcttccgc gcgtttacaa gtcaccgttt ccacggcaaa acaaaaaaac aacgccgtca
     9301 attgcgtaaa gcaagcatgg tttctagtgg cgacttcaaa cgtattcgcc aacaactagc
     9361 aaaaatgaaa taagaagtac aaataacaga atctgaagat ttatttttat taggaggaat
     9421 tatacatggc acgtgttaaa ggtggaacag taactcgtaa acgtcgtaaa agagtgctaa
     9481 aattagcaaa aggttactac ggttcaaaac acactttatt taaaacagca aaagaacaag
     9541 taatgaattc atacaactac gcatatcgcg atcgtcgtca aaagaaacgc gacttccgta
     9601 aattgtggat tgctcgtatc aacgcagcag ctcgtatgaa tggcttgagc tactcaaaat
     9661 taatgcacgg tttgaaagtt gctgaaatcg acatcaaccg taaaatgtta gctgacttgg
     9721 ctgttaacga tgcagcagca tttactgcat tggctgacca agcaaaagaa gctttggcta
     9781 aataagctaa acaattataa agaggaaaga agataacgaa aagttatgtt ctttcctctt
     9841 tttttattta caatcctcca tcaaatgaaa caggacaata acgtataagt ctaaaataac
     9901 ataattaagg ttttttaatt gaaaactgaa catttatggt catcctttaa tgaaagcgct
     9961 aactacataa gttagaatga gtttgactga tatattagtt tgcttatcag aaaaacaaaa
    10021 tggacggagg aataacatga gtgatattat tattggtatt ttgttagtta tctcattctt
    10081 tttcatggtc tggtactgtg taaaaggcta caacttgatg gtcggctttt gtcatcatgg
    10141 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac tcggcgatgg
    10201 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
    10261 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
    10321 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
    10381 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
    10441 gaccagtgat ttctattgca gttattgtgt tgccgatctt gatgtcatta ggtatttcag
    10501 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
    10561 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
    10621 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
    10681 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
    10741 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
    10801 gggtcgttct tttagatctt caaatcatct taggttttat tctagcaggt atctgggcat
    10861 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
    10921 tatttacaga tggcgcagtc gatgttgcac caatggttgg tttcttgatg accttagcga
    10981 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
    11041 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
    11101 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtgttagctg
    11161 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaattaca acgattgctc
    11221 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
    11281 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcggt gcgatcttat
    11341 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
    11401 tagataaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
    11461 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
    11521 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
    11581 aaattgtttg cgggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
    11641 cacacaagcg actaatgcac tgattgaagg agatgtagca aaagtcggcg taatcgggat
    11701 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
    11761 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aaaacttaaa
    11821 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
    11881 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
    11941 atctgtgaaa gcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
    12001 aagaagaact cggacagcag caatcaacgc atctatttta ccaaaaatgc tagatacggc
    12061 aacctcgaca gaagactccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
    12121 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
    12181 ttccggtcct gctgcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
    12241 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
    12301 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
    12361 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
    12421 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
    12481 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
    12541 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgttct
    12601 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
    12661 acaggcatta gcagactatt gtcatacgac ggttgaagac atcgcagagc agatcatgga
    12721 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
    12781 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
    12841 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
    12901 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
    12961 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
    13021 accagaatcg atcgaagtcc acgtagaaat cgatccacat acttcaaaag taacggctat
    13081 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
    13141 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
    13201 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
    13261 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
    13321 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
    13381 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
    13441 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
    13501 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
    13561 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
    13621 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
    13681 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
    13741 gagaaatcta tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
    13801 aacgaatcac ttttgcaact tatgcagaag atgaagggat ccgattgatg acggagccgt
    13861 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
    13921 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
    13981 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
    14041 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
    14101 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
    14161 aagaaataaa agaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
    14221 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
    14281 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
    14341 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaaaggtg ttcaaaagct
    14401 tgcatctgcg gtaataagaa cagccaagtt aaaaacataa gaagctattt ttacttggct
    14461 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
    14521 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
    14581 tatttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
    14641 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
    14701 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
    14761 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
    14821 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
    14881 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
    14941 cttttttatt ttgagcgatc actgcctgat cggtttgttc tagtattttt tctagtttag
    15001 cttgatcttg cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
    15061 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
    15121 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
    15181 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
    15241 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
    15301 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
    15361 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
    15421 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
    15481 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
    15541 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
    15601 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
    15661 atacaagaaa aatgcttaga cggtatttat taagaattta catcatccaa agaagcgtac
    15721 gcttggaata actgttctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
    15781 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
    15841 cccacaattt cgtagctaaa atccaaccgt gttcccgtat acttttggat agttgggtgg
    15901 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
    15961 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
    16021 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
    16081 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
    16141 tcgttttcca ttgatttttt acaaaaataa ttcatccact tcggaagtgc ttcgctgatt
    16201 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
    16261 taaacagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
    16321 atcccagcta aagtgtcacc catgccgcct gttgccatag cagcagaacc taaaggattg
    16381 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
    16441 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
    16501 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
    16561 aaaagatgat tagcaacaag taaacttaaa gcagagccat caatcaccag ccattgttct
    16621 tttgattgtt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
    16681 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
    16741 gtccaatcta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
    16801 tcagttacta cagtcgtgag acccgctcca ctgtgtacgg cagctaaagc actcataatg
    16861 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
    16921 tgtgaaatct ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
    16981 gaaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
    17041 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
    17101 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
    17161 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
    17221 gattcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
    17281 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
    17341 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
    17401 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
    17461 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
    17521 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
    17581 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
    17641 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
    17701 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
    17761 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
    17821 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
    17881 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
    17941 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
    18001 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
    18061 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
    18121 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
    18181 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
    18241 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
    18301 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
    18361 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
    18421 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
    18481 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
    18541 aaataaaaat actaaaaaac tccggctatc tctgacagct ggagttttta tgtttaaaaa
    18601 tgaattagta catcggtatt cttaaaagta atttagttcg tcttttgatt tctcaggagg
    18661 gatcaaaaag gcgaaagcac taagtatagc aaaacttgtg atgatttgcg tacgatacgt
    18721 aaaccaataa tgaatctgtg agtgattagc taaaatattg taccaaatat acggcgttac
    18781 agcagctaaa acaaacaaaa ctagaagttg gaacggacgc cagctatttc gtttacgata
    18841 agcaaaatag agaaaaacaa gacaagtaat caatagcaat aatattgcta cttttggata
    18901 catcaatcta atattgcttt tcagcatctc taatcgatgc aacgggtatt tgtcgtttcc
    18961 ttccgttcga aagaggattt ggttgattgc atcttttaca atgtttttgt tcaatatgat
    19021 cgatgatagg atccatttag ccacccatgt caaaccatag cctaatcccc aaaagatacc
    19081 ggtaaaaaca aaactgccta cttgtttgct caatgaactg cgtttatcca ttaaatactt
    19141 atttgttaca taataaataa tgatcacagg tatcccccaa gagatgaccg gtgttgtcaa
    19201 caagtcaaaa aagttagcta aagaaccaat gacaaagaaa tacaagaata attgttggtt
    19261 ggtgttggag agttgtggtt ttgttaaaaa tagtaataca gacaataaag tcaaaatcca
    19321 aatatttgaa aattgcatac tgaagaaaaa ggttgcagca tttcctatag caagcgaaat
    19381 caacaaggct gtcgttaaat agatgtcgct acacttggtt aaaaagtagc tcgtcaatcc
    19441 aatcagcaga aaaagaatgg ctgcgtaaat cattcgaata ctccccaaac tcatccctag
    19501 cagtaaaggt cgtaaaaaaa gctgataacc atgccaataa cggggataat ccgccataga
    19561 catcgcgttc tccaaggagt tcaattcagg atctgctttt gtttttcgta tcatcaaatg
    19621 atcagtaaaa ttatctaacc gagtgcctag catatttcct tgattgatag aaggatacat
    19681 tccttctttt tcaaccatct gtagagattt ttctgcattc gattcaatag agtgctgcgg
    19741 aatgaaaaaa gtaattgtca gtagtacgca atagctagcg atcaagagaa caaaagcaaa
    19801 aaatatcttt ttaaatagtt tcatgagtat ctcctatatc tgttttcatt ttttctcttt
    19861 tatctagtaa aaaaggagag aggatataaa caggtagacc taatgctaaa acaaacacat
    19921 accgtaaact aaaagccaca ggagtagcaa tcataatagt agcaaaaaca agcataccgg
    19981 gaagaaaaag gagtaaatac cgatattttt tcttaaagat tgctaaaagt gcactgccga
    20041 tagctaacca cagtaatgtg ccggaaccaa agaaatcacg agcatcgatg atttttttca
    20101 ttcctgacaa tccagtaaga tgttcaatca aatctacttg cttcatacca taattattcg
    20161 tgtttacacg agtatctaaa aagccgtaac tattttttgt accaggtacc cagaacccga
    20221 acgtttctaa aatataagca tctgtataga tttttatatt acttggtaaa tttcttgccc
    20281 atagtgatag aaattctttt ttatgtgtat tcaaaaactc attgttaaaa tttttatgcc
    20341 acttgattga gtctgcaaga ctaggagaat acgcttcata atcttttatc gggagtagct
    20401 gattcatgaa tgctttatct ttcttagaca agtcaccatc cataacaata gttctagaaa
    20461 tttgctgcaa aggaacacct aattgttcag cagtttctgt aggaatagca taacgagcca
    20521 tcaacgggtg gatgagtagt ccagcaatca ctgtataacc cagcaccact tgaaagaatg
    20581 cttgatacct tttttgtttc aaaataatca agccgcaaat caaacctgtg ccggttacta
    20641 cataaatccc attatttctt agataggtgg tcagtaaagc aaggatgatt acgtaaatct
    20701 gattgagaat cgttggcttt tctcgttgaa aaataaagtc aaaaaccgct aaggaaaata
    20761 aaaagcaagc gatagagaac aaagtatctt tttgcatgtt caaggcgtag attggaaaaa
    20821 ccggtgcaaa tgcaaaatag cataaactaa tgagtaccgg aaaaaaatgg atgtttcttt
    20881 ttaacagcca gcaaagagaa taacttatcg ctgaagctgt tatcaatgtt tgaaaaatcg
    20941 aaaataaaaa tacaccgtga ttgatcgtat cagttggtaa atcatgaaca aatagcttga
    21001 ccagtaaggt gaataagact ggatgatgat tgcttatagg aacatcaccc atagattggg
    21061 cgaccgaact taacgaatca ggcaaaacca ctccaggata caatgccaac agataaggaa
    21121 gccaacacag aactaaaaag acagtaaaga ataaaaaata ttttcttgaa gagaaagcct
    21181 gtgtgttttg agcagtaaaa aatgtttcca gttttggcat tgataaataa atcgattgta
    21241 aaaataaaaa agaatacaca aaaatggaga aataaacaag aaagtaaaaa atacttccat
    21301 cagcaatcgt tgtctttgta tagtttgctg agacatactg aaattgtatc tgtctgccaa
    21361 tagaaagtgt aagagcaaaa gctgcagaaa aaaacaatcg ggcaatagct gcaactttgt
    21421 tttttattga aaacaactct attccatatt gcttaaaagc aagtagcaaa accatggcta
    21481 aaccaatgat tactgttgga ttcaaagttc ctagaccata agaagtaaaa aaagcaaaac
    21541 ctgacgcaaa taaagttgca aaaaaagtag gaaatagatt ctcctttttc ttatgcataa
    21601 aaaaggcctc ctacttcatt tctttatttt gatattcgtg gtagacatgg tataaattca
    21661 attcatattc tttcttggca tttgtgacga ccgtatccaa aatcaaccct gttactaatg
    21721 aaagaagggc taaagtcatc aatccagtac ttaaaatcgc agaagggatg cgtgtaataa
    21781 agcttgtcag catatattca cgaatcacag gaatccccat gaccaatcca aataaaaaga
    21841 agaagatggt ccagatccca aagaaaagca aaggtttata atctttgcac attttgatga
    21901 tcatcatgat cactttgaat ccgtcagaga atgtattcaa ttttgattcg ctaccttcag
    21961 gacgatcgcg ataatcgatt ggaatctcaa cgaacttgaa ttttttatct aaagcatgaa
    22021 tcgtcaattc tgtttcgatt tgaaaaccag aactcataat agggaagctt ttgacaaaaa
    22081 ttcgattgaa tcctcgatag ccggtcatga catcgcgaat ttttgtttta tataagtaag
    22141 taattgtatt tttaaccaga ttattcccaa aatcatggaa aggacgctta ttttcatcaa
    22201 aataggttcc atttgataga cgatcgccaa tgaccatatc tgcttcgcca gaacgtaatt
    22261 tctcaagcaa accatgtgca gcttctgcag gataagtatc atcaccatct accatcaaat
    22321 aataatcggc atcaatatca aaaaacattt gacggattac attccctttt ccttgacgtg
    22381 gttctttctt tacgattgct ccgccttgaa cggccaattc gtatgttcga tcggtcgaat
    22441 tattatcata gacataaata tctgcttctg gtaattccct tttaaaatct tcaataacag
    22501 ttgaaattgt cgcctcttcg ttataacaag gaatgagcac ggcaattttt tcacgtgatt
    22561 ccatttgtca ctctccttat ataataaaac taatcttatt tcaataatac cgtataataa
    22621 ctacaaatac tagctattcc aagaggaaat agccctattt ttataaaaat cacccactat
    22681 aaaagaagga taggatacct ctcttttaga tagtgggtgc gtattcgtta tttcattttt
    22741 ttgacgtatt aatctattgc gccctttttt cttgaaatgt ctaatacggt tcctgtatga
    22801 gcatctgcta aaaattcata ataaacagtt tcgccatctt ctatccggga aagcccaccc
    22861 cgataagcac tcgaccgaac ggcgaatttc cgaataggtt ctttttcaaa agagatccaa
    22921 gcaccttcga ttgcgccttc ttttaaaaaa gatgttttga tattttcaag cacttggtct
    22981 gctgataaca cttttttgcg atgataccaa atcgttgaag ctaccccact caataatcct
    23041 agaccgatcc caacggctaa cccgccttta aaatagctta attcgctttc ttcatacatg
    23101 tggatatacc tccttcttct cacttgctac tctttgtgtt tattttacca cactctcata
    23161 ttttctttga aataaataac cgctaccaga aaaaataagc tataataaat acgatcggtc
    23221 tgcagaaaaa atcatatttt atccgcagat caatataagt ctgagaggag tttagtctga
    23281 aatttagacg aatagcatat ggaagaaaaa acatttcaac gaatcaaaga gttaacagaa
    23341 ttacaaggaa caagcggttt tgaacaagac gttcgcgcct atatggaaag tcatatggaa
    23401 ccgctggtag atgagttaca gttagatggt ttgggcggca tttttgggct gcgccaccat
    23461 gaagaagcgg atgcacctcg tgtaatggtc gcagcacata tggatgaagt cggattcatg
    23521 ctgacacaaa tccaagaaaa cgggcttttt aaagtcgtac ctctaggcgg atggaaccct
    23581 tacgttgttt ctgctcaacg ttttacactt aaaacctcta ctggtaagaa ttatccatgt
    23641 atttcttctt ccgttccccc acatttatta cgaggaacaa gcggacaaaa atcagtagaa
    23701 gtaacagata ttttgttcga tgctgggttt gaatcaagag aagaggcaat gagttacggt
    23761 gttcttccag gagacacaat cgttccttac gctgaaacga tcaaaacagc caacgggaag
    23821 aatatcatca gcaaatcgtg ggacaaccgc tatggctgta caatggtatt agaagcttta
    23881 gaagcgttac aaaatgaaac attaggtcat acattgattg caggcgcgaa tgtccaagaa
    23941 gaagtaggct tgcgtggttc taaagcttct gtcaataaat tcaagccaga tctgttcttt
    24001 gcagtagact gctctgcagc agatgacaca gtgacgaaaa atggcacatt cggacattta
    24061 ggagaaggta cattgatgcg tatccaagat ccaggtttga tcatgcttcc tcgtttgcgt
    24121 gagtacttgc tagatatcgc tgaaacaaac aacatccctt atcaatactt tgtttcaaaa
    24181 ggcggtacag atgcaggtgc tgctcataca caaaatgaag gaatcccaag tacagtgatt
    24241 ggcgtagtgg gacgttatat ccatacgcat caaacgatgt tcagtattcg cgattttgaa
    24301 gctgcacgag aaatgttgat tcaaacattg aaaggtttag ataaatcaac agtgaacacg
    24361 atcgtatacg ggaaataacg cgatagtgaa actataaaat ctggcaaaag caaccttttg
    24421 tcagattttt tttataaaaa gaggatcaat gtttgttagt tcgaaagaaa ggtgtataat
    24481 ttaattgaaa aatacataaa cagaaagtag aggaaaaaga atgattatcc caaatagttt
    24541 agaagaatta gcaggctacg tagaaactgg aaaaagtgta tttttcttta ctgccggctg
    24601 gtgcggagac tgccgtttta tcaaaccgca aatgcctgaa atcgaaaatg acttttctga
    24661 ttggcaattt attgaagtag accgtgacaa gtacatagat gtagccgctg aatggaacat
    24721 ttttggtatc ccaagttttg tcgtgatcca agacggaaaa gaattaggtc gtctagtgaa
    24781 taaagatcgt aaaacaaaac aggaaatcga atcgttccta gaaagtgtaa taaaaggctg
    24841 acatctgtta ggaggggtca agatgaatca agaatataag aacattttag taggtattga
    24901 cggtagtgaa caagcacttg ctgctttcaa aaaagcgtta gaagtggctc gccgtaataa
    24961 tgggaaagta tatgtagcaa acgtaatcga tcagcaattt tataatttta tgggctatgc
    25021 tcctattgat caaaatctag tcgatcagca aactgaaaca gccaagcaaa tgatcgaaga
    25081 atgtaaagaa tatggaaaat ctgttgatta cacaaatata gaaggaatta ttgcatacgg
    25141 ctctgttcgt gaatccatgg cagtcaaatt gccaaaaaaa tatgcaatcg atctaatcat
    25201 ggttgggcag tctggactaa atgcagtcga acgctttatg acaggaagtg tagccagcta
    25261 cgtcatacgt cattcacctt gtgatgtctt gatcgtttct gacgaagaag aacagccaag
    25321 taattaagca agtggaactt gtataacctt tttaatcaaa cagtaaaggg actgtgacaa
    25381 aagtcttgtc acagtccctt gttacgaata aatggtgttc cagaagaagc ttcttcggaa
    25441 ataaagcatg aaaaaaatct catgcagtga atgcaatttt ttgttaagat aaatctcagc
    25501 aaaggataat ggaggaaaga acatgatttt tgcttataac aaagcacacg tcggagacac
    25561 tttgatggtt atcatagccg acgataaagg aacagaaaac actgtcgaac gtaaatggaa
    25621 tgttgctcgg atcaaagatg aacaaggtca agttgttgct tggaactttt tccatatctc
    25681 cgatcatcta acaattgaag ggaacggcca agtaagctta acagatgaag aattagcgaa
    25741 attgaatcga ttgattaaag aagcaggatt cgaagaacag cttgaagctg atcatgaacc
    25801 gaagatcgtt gtcgggtatg taaaaacatg cgtcccgcat cctgactctg accatttatc
    25861 gattactgag acagaagtgg acaatgggac agtattgcaa attgtttgcg gtgcaccaaa
    25921 tatcgaagct ggacaaaaag ttgtcgttgc aaaaccagga gccatgatgc cagatggaat
    25981 gatgatctgg ccaggaactt tgcgaggggt agccagctat ggaatgattt gttctgctcg
    26041 tgaattgcat ttgccaaatg ctccagaaaa aaaaggaatc ttagtattgc ctgaggatgc
    26101 cgtagtcgga gaagtatttc caaaataaag aggttgtgac aagtctttgt cacaacctct
    26161 ttattattaa ttttcactat tttgattttg ttttaatgcc gattgatcga tcgtcaattc
    26221 aacagtagtt gttttttctt tcgagccttc atagtaagtg attttgacag aatctcccac
    26281 ttttttctta tacaaagcag attgaagttc tacaccagaa gaaacttctt tgtcatcgat
    26341 cttagtaatg acgtcgtatt gtttcaaccc agctttttct gccggtgtag ctgtttggac
    26401 atttgtcacg atgacaccgt tagttacaga tgaaggaatc ttcaagattt gttcttgttg
    26461 ctgagtagaa acggcagata agtcgaccat tgtgatacct agagctggac gtgtcacttt
    26521 tccgtctttt tccagttggt tgatgatatt cactacatcg ttacttggga tggcaaatcc
    26581 cattccttct acgcttacat tagaagtcga ttcagaagta cttgcgatct tacttgagtt
    26641 gataccgata acttgtcctt cgatattcac taaaggacca ccagaatttc ccgggttgat
    26701 cgctgcatct gtttggatcg cattgatatt gactgtttcg ttcgattcat tcgtacttgt
    26761 tacttgtcgg ttcaaggaag aaatgattcc tgaagtgaca gaattcgcat attcagatcc
    26821 taatggggaa ccaatcgcaa tcgcaggttc accaaccttc aaagcacttg agtccccaaa
    26881 agaggcaacc gtttcgactt tgtctgaatt gatcttcaac accgctaaat ctgaataggc
    26941 atctgttcca actaattcag cttttacctt cgtaccgtct ttcatcatga cttccaaacc
    27001 ttgctgtcca tctactacgt gattgtttgt gactacataa gcagtattcc cgtctttttt
    27061 gtagattacg ccgcttcctt cactataagc ttccaaatta ctatcatctg agctgctact
    27121 ttcttcttgc tgcccgaata atccgccaaa accgcttgat tggttttgac tttgtaagtt
    27181 gataacagaa acgacagcgt cttgaacttt atctaccgct ttggtgatat cagaatctac
    27241 attgactttg acattttcca caactgtttc accggcagaa ttttgattgc cgcttgtagc
    27301 agtagaacta ttgttgccag tgcccatcgc tgcataaaaa atgccagctg tcagtagtcc
    27361 tccaacgatc cctccaacaa gccccagacc caacttacgc cagaggctat tatttttatt
    27421 ttttttcatc tttggtgtca cattttttct atccatgtgt tcctcctcca attacattca
    27481 tttatctact gttagtatag tcttatttgt tgactttagt ctactttgag actttgaact
    27541 ttttatgaaa aactttagga aaaatcattg ttttcagatg aaaatatttg attttctttt
    27601 tccacagata ttgtgtaaaa ctacgcggat aggattggaa aaataaggga taaaacatat
    27661 ccacagcctg tgtataaagt ggataaattc ttataaaacg tatgtttgcc taatgaatgt
    27721 gatgaaataa ggtaaaacaa cgctgttttc tgtggataaa cctgtggatg ttgtgcataa
    27781 ctgttgggga aatcttttta acaaaaatag tcaaaataaa catatagacg aaaaaattga
    27841 ttataataaa caaagtgagc gtgaagatca aaggagagaa aacatgaata tcaaaattat
    27901 ttcagtagga aaattaaaag aaaaatactt gatccaaggt atcaatgagt atgtcaaacg
    27961 gctgaatgcc tatgcgaaaa tcgaattgat cgaagttcct gacgaaaaag ccccggagaa
    28021 tttaagcgaa gcgcaaatga gacaagtaaa agaaaaagaa ggagaacgga tcttagcgaa
    28081 aatcaaagaa caagaatacg tctatggctt agcaattgaa gggaaaaatc ccacaagcga
    28141 atcttttgct aaacaaatcg accaattagg tatccaagga aaaagccacc ttgtttttgt
    28201 tatcggcggt tctttaggac tcagcgaagc cgtcatgaaa agaagcaatg ctcagatctc
    28261 ttttggcaaa atgacgtatc cacatcaatt gatgcgcctg atcctcgtcg agcaaatcta
    28321 ccgtgccttc cggatcaacg cgggggctcc ataccacaaa taatgatatt ttttaagaga
    28381 aaaatagcat aaaaatctag ttatccgcat aaaaactgga cttatcacac tttatcaagg
    28441 tcaaaaccac tcaatttact actaatttac tacttatgaa tgagctttga tacgacgatt
    28501 tatccttgaa aagtgaagat ataaagatac ttccaataaa atttgaatat ttaataggta
    28561 gacacttcaa aaaatgaggt gtctattttt ttacccgatt ttgaaaggaa gtgaacttat
    28621 gaaaacaaaa aatcaagaat caaaaggtcg ttccccactc tttaagacca tcaaacattc
    28681 attcagccaa taaaaaagaa aggataggta aaaatatgga acttaaattt gtgattccca
    28741 acatggaaaa aacattcggc aatttagaat ttgctggcga ggataaagtc gttcagcgaa
    28801 gaatcaacgg acggctaact gtcttatcaa gaagctataa tctctattct gatgttcaaa
    28861 gagcagatga tattgtggtg gtgcttcctg ctgaagctgg cgaaaaacat ttcggctttg
    28921 aggaacgtgt gaagttagtc aatccacgta ttaccgcaga gggctacaaa atcggcactc
    28981 gtggttttac aaattacctt ttacatgctg acgacatgat aaaagaataa agaaagagag
    29041 gaaaaatgat gagattagca aatggcattg tattagataa agacacgact tttggagaat
    29101 tgaaattctc tgctctacgt cgtgaagtga gaatccaaaa tgaagacggg tcggtttcag
    29161 atgaaatcaa ggaacgtacc tatgacttaa aatccaaagg acaaggacgc atgattcaag
    29221 taagtattcc tgccagcgtg cctttgaaag agtttgatta taacgcacgg gtggaactta
    29281 tcaatcccat tgcggacacc gttgctactg ccacctatca aggagcagat gttgactggt
    29341 atatcaaggc agacgatatt gtgctgacaa aggattctag ttcattcaaa gctcaaccac
    29401 aagcaaagaa agaaccgaca caagacaaat agtcgctagg tagaaaggag actttttcgc
    29461 atgaaacagc gtggtaaaag gattcgccca tctggtaaag atttagtctt tcattttacg
    29521 atagcgtcac tcctgcctgt tttcctgctg gttgtcggac tgtttcatgt gaagacaatc
    29581 cagcagatca actggcagga ttttaaccta tcacaagcag ataagattga cattccctat
    29641 ttaattatca gtttcagtgt cgcaattctt atctgcttgc tggtagcgtt tgtattcaaa
    29701 cgggttcgct atgatacggt taaacaactt taccaccgtc aaaaactggc aaagatgata
    29761 cttgaaaaca agtggtatga atctgaacag gtcaaaacag agggtttctt taaagatagt
    29821 gctggtcgta caaaggaaaa gataacctac ttccctaaaa tgtattatcg acttaaaaat
    29881 ggcttgatac agatacgggt ggaaatcacg ctgggaaaat atcaagacca actcttacac
    29941 ttggaaaaga aattagagag tggcttgtac tgtgagctga cggataaaga gttaaaggat
    30001 tcctatgtgg aatatacttt gctctatgac accatagcca gtcgtatttc tattgatgaa
    30061 gtagaagcta aagatggtaa acttcgctta atgaaaaacg tatggtggga atatgataag
    30121 ctccctcata tgttgattgc tggtggtaca ggtggcggta aaacttactt tatactgaca
    30181 ctgattgaag ccttgcttca tacagattca aaactgtata ttcttgaccc gaaaaatgct
    30241 gaccttgcgg acttaggttc tgtgatggca aatgtctact atagaaaaga agacttgctt
    30301 tcttgcattg aaacattcta tgaagaaatg atgaaacgta gtgaggaaat gaagcagatg
    30361 aagaactata agactggcaa aaattatgct tacttaggtc tcccggcaca cttcttaatc
    30421 tttgatgaat acgtcgcttt catggaaatg ctgggaacaa aagaaaacac cgcagttatg
    30481 aataagctga aacagattgt catgttaggt cgtcaagctg gcttctttct aatactggct
    30541 tgtcaacgtc cagacgcaaa atatttaggc gacggaatcc gtgatcagtt taatttcaga
    30601 gtggctttag gtcgtatgtc tgaaatgggc tatggcatga tgtttggcag tgacgtacaa
    30661 aaggatttct tcttaaagcg aatcaaaggt cgtggctatg ttgatgtagg aacaagtgtc
    30721 atatcagagt tttatactcc ccttgtacca aaaggatatg atttcttgga ggaaattaaa
    30781 aagttatcca acagcagaca gtccacgcag gcgacgtgcg aagcggaagt cgcaggtgtg
    30841 gactgatctt gctggctggt gtggcaatag ccacgccagc acttaacccc tcggtatcta
    30901 acaggggggt acaaatcgac aggaaacagt caaaaaaaca ttagaaaatc ctttggttac
    30961 aagggattta caaaatttca gcgtatgtca aatgggcttt aaaagttgac atacgccttt
    31021 ttgattggag ggatttttac tgaatgaaca aacttggtta cagcatttaa aagaaaaacg
    31081 cttggcttat ggactatctc aaaaccgttt agctgttgcg actggtatta caaggcagta
    31141 tctaagcgat attgaaacag gaaaagtcaa gccatcagag gatttacagc agtccctttg
    31201 ggaagctctg gaacgcttca atcccgacgc tccccttgaa atgctgtttg attatgtaag
    31261 aattcgcttt ccgacaacag acgtacagca ggtggtcgaa aacatcttac aactgaaact
    31321 gtcctatttt cttcatgagg actatggttt ctattcttat tcagagcatt atgctttagg
    31381 cgacatattc gtcctttgct cccatgaact ggacaaagga gttctggtgg aattgaaagg
    31441 tcgtgggtgc agacaatttg aaagctatct tctggcacaa caaagaagct ggtatgagtt
    31501 ctttatggac gttttggtgg ctggcggtgt gatgaaacgc cttgaccttg ccattaacga
    31561 taagacaggg attttaaata tccctgtact cactgaaaag tgccaacagg aagaatgtat
    31621 ctccgtcttc cgcagtttta aaagctatcg cagtggcgaa ctggtacgca aagaggaaaa
    31681 ggaatgtatg ggaaacaccc tctatatcgg ttcattacaa agtgaagttt atttctgtat
    31741 ctatgaaaag gactacgagc agtacaagaa aaatgatatt cccattgaag acgcagaagt
    31801 aaaaaaccgt tttgagattc gattgaaaaa tgagcgtgcc tattatgcag tccgtgattt
    31861 actcgtctat gacaatccag agcataccgc ctttaaaatt atcaatcggt atatccgttt
    31921 tgtagataaa gacgattcca aacctcgttc tgattggaaa ctgaatgaag aatgggcttg
    31981 gtttattggg aacaatcgtg aacgattaaa actaaccaca aaaccagagc cttactcctt
    32041 ccaaaggacg ctgaactggc tatctcatca agttgccccg accttaaagg ttgcgattaa
    32101 acttgatgaa atcaaccaga cgcaggttgt aaaagacatt ctcgaccatg cgaaactgac
    32161 agaccgacac aagcagattt tgaagcaaca gtcagtaaaa gaacaggacg tgataacaac
    32221 aaaaaaataa ctcaaataca aattcattga atatagagag gagaacattt ttatgaattt
    32281 tggacaaaac ct
//
LOCUS       DACEUF010000025        31551 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.026, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000025 DACEUF010000000
VERSION     DACEUF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.026"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(862..1203)
                     /locus_tag="IXG20_001125"
     CDS             complement(862..1203)
                     /locus_tag="IXG20_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1609335.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1203..1433)
                     /locus_tag="IXG20_001126"
     CDS             complement(1203..1433)
                     /locus_tag="IXG20_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1609336.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1720..2058
                     /locus_tag="IXG20_001127"
     CDS             1720..2058
                     /locus_tag="IXG20_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609337.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2263..2502)
                     /locus_tag="IXG20_001128"
     CDS             complement(2263..2502)
                     /locus_tag="IXG20_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ1609338.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2478..2825)
                     /locus_tag="IXG20_001129"
     CDS             complement(2478..2825)
                     /locus_tag="IXG20_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ1609339.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(3557..4138)
                     /locus_tag="IXG20_001130"
     CDS             complement(3557..4138)
                     /locus_tag="IXG20_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1609340.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4321..4947)
                     /locus_tag="IXG20_001131"
     CDS             complement(4321..4947)
                     /locus_tag="IXG20_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1609341.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4969..6300)
                     /locus_tag="IXG20_001132"
     CDS             complement(4969..6300)
                     /locus_tag="IXG20_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ1609342.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6455..6856)
                     /locus_tag="IXG20_001133"
     CDS             complement(6455..6856)
                     /locus_tag="IXG20_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1609343.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6942..7148)
                     /locus_tag="IXG20_001134"
     CDS             complement(6942..7148)
                     /locus_tag="IXG20_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609344.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7243..8745)
                     /locus_tag="IXG20_001135"
     CDS             complement(7243..8745)
                     /locus_tag="IXG20_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ1609345.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8763..9218)
                     /gene="argR"
                     /locus_tag="IXG20_001136"
     CDS             complement(8763..9218)
                     /gene="argR"
                     /locus_tag="IXG20_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1609346.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9316..9933)
                     /locus_tag="IXG20_001137"
     CDS             complement(9316..9933)
                     /locus_tag="IXG20_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609347.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10154..11527
                     /locus_tag="IXG20_001138"
     CDS             10154..11527
                     /locus_tag="IXG20_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1609348.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11602..12714
                     /locus_tag="IXG20_001139"
     CDS             11602..12714
                     /locus_tag="IXG20_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ1609349.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12786..14423)
                     /locus_tag="IXG20_001140"
     CDS             complement(12786..14423)
                     /locus_tag="IXG20_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ1609350.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14591..15199)
                     /locus_tag="IXG20_001141"
     CDS             complement(14591..15199)
                     /locus_tag="IXG20_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1609351.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(15583..16734)
                     /gene="queG"
                     /locus_tag="IXG20_001142"
                     /pseudo
     CDS             complement(15583..16734)
                     /gene="queG"
                     /locus_tag="IXG20_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
     gene            complement(16753..17505)
                     /locus_tag="IXG20_001143"
     CDS             complement(16753..17505)
                     /locus_tag="IXG20_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609352.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(17646..18596)
                     /locus_tag="IXG20_001144"
     CDS             complement(17646..18596)
                     /locus_tag="IXG20_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1609353.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(18597..19436)
                     /locus_tag="IXG20_001145"
     CDS             complement(18597..19436)
                     /locus_tag="IXG20_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1609354.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(19433..20179)
                     /locus_tag="IXG20_001146"
     CDS             complement(19433..20179)
                     /locus_tag="IXG20_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609355.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            20417..21787
                     /locus_tag="IXG20_001147"
     CDS             20417..21787
                     /locus_tag="IXG20_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1609356.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(21842..22738)
                     /locus_tag="IXG20_001148"
     CDS             complement(21842..22738)
                     /locus_tag="IXG20_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1609357.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(23019..23696)
                     /locus_tag="IXG20_001149"
     CDS             complement(23019..23696)
                     /locus_tag="IXG20_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1609358.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(23787..24692)
                     /locus_tag="IXG20_001150"
     CDS             complement(23787..24692)
                     /locus_tag="IXG20_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1609359.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTAVSWTT"
     gene            24867..26321
                     /locus_tag="IXG20_001151"
     CDS             24867..26321
                     /locus_tag="IXG20_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1609360.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            26337..27368
                     /locus_tag="IXG20_001152"
     CDS             26337..27368
                     /locus_tag="IXG20_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1609361.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            27375..28562
                     /locus_tag="IXG20_001153"
     CDS             27375..28562
                     /locus_tag="IXG20_001153"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ1609362.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            28718..30100
                     /locus_tag="IXG20_001154"
     CDS             28718..30100
                     /locus_tag="IXG20_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1609363.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            30072..30458
                     /locus_tag="IXG20_001155"
     CDS             30072..30458
                     /locus_tag="IXG20_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609364.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(30658..31326)
                     /locus_tag="IXG20_001156"
     CDS             complement(30658..31326)
                     /locus_tag="IXG20_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ1609365.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8917 a         6390 c         5693 g        10551 t
ORIGIN      
        1 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
       61 aataatattg acaaaaagtg actcaaaata aaaaaaaact tacattatac tggaatgaac
      121 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
      181 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
      241 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
      301 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
      361 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
      421 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
      481 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
      541 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
      601 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
      661 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
      721 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
      781 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
      841 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
      901 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
      961 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
     1021 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
     1081 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     1141 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     1201 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     1261 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
     1321 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     1381 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
     1441 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
     1501 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
     1561 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
     1621 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
     1681 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
     1741 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
     1801 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
     1861 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
     1921 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
     1981 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
     2041 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
     2101 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
     2161 tatatgcagc ttatgagtat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
     2221 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
     2281 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
     2341 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
     2401 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
     2461 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
     2521 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
     2581 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
     2641 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
     2701 agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc
     2761 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
     2821 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
     2881 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
     2941 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
     3001 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
     3061 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
     3121 gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct
     3181 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
     3241 tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca
     3301 acctctccac taaacgatgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa
     3361 aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca
     3421 caacctctaa tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac
     3481 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
     3541 tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt
     3601 gaaaattctg cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct
     3661 ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggat agtttttctt
     3721 cagaaagtgc caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta
     3781 gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac
     3841 agccatgata agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta
     3901 atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata
     3961 cgtccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat
     4021 aaaattgacc ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat
     4081 agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg
     4141 atattctcct ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct
     4201 tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg
     4261 ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc
     4321 ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa
     4381 gttcgtcaac gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac
     4441 cacaacattg aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc
     4501 gttcattcca ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc
     4561 attgctttct ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata
     4621 aggtttcaat tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact
     4681 ttttctagca aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca
     4741 tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt
     4801 tgcttgtttt accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt
     4861 tgtcaaacca aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac
     4921 tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac
     4981 atcatttaga gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata
     5041 aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc
     5101 atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc
     5161 tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt
     5221 gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg
     5281 agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc
     5341 cttcaactga tccataatga ttcgataatc atctagagag atataagtga actgcaatcc
     5401 gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac
     5461 agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt
     5521 acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc
     5581 atcttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac
     5641 acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc
     5701 ttcatcttca cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta
     5761 ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt
     5821 ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt
     5881 caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc
     5941 ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg
     6001 tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc
     6061 gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct
     6121 ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc
     6181 atacatacgt gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt
     6241 cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat
     6301 ttttccctcg cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt
     6361 ctattctcca aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc
     6421 acgtttttag tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga
     6481 tatttcctct agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt
     6541 gggcttcttc ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc
     6601 cgccatcatc taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc
     6661 ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa
     6721 ataattgttc tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca
     6781 tcgagccatt tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt
     6841 cgtatgcttt tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt
     6901 atagtgtatg acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg
     6961 ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata
     7021 tttgttccaa aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga
     7081 tacatatata ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc
     7141 gttaccattt tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac
     7201 tttttttgcc gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga
     7261 ttcaagatca gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt
     7321 tccgaattca tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat
     7381 caaaaaccag ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc
     7441 cgggtcgaag ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt
     7501 gtttccttgt tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac
     7561 gggctgtttc aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg
     7621 caaaagcatc aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt
     7681 gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag
     7741 ttctaatgct ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc
     7801 tttttcttca atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt
     7861 tcctggatgc tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat
     7921 cactgttcca gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc
     7981 aagatctgct tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc
     8041 aggatttggg atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga
     8101 atccgcacct aagctattca atactttttc agcaaagaat ccaccagcac catttccggc
     8161 atcaacaatg attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt
     8221 aatttttgtg actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc
     8281 agtttgttcg ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc
     8341 tgctcctcct tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc
     8401 agtcagcata accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc
     8461 aggcgtagta gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat
     8521 caacgcctct ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca
     8581 ttttttattt ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc
     8641 gatctttttt accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat
     8701 tcctcgaata tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc
     8761 atttattccc tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg
     8821 caaataataa tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact
     8881 tcatctagcc aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg
     8941 aactgaacag actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca
     9001 tttaaagaaa cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga
     9061 ctttttacta acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg
     9121 cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg
     9181 atctggcgga tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga
     9241 ttatagcaaa agataagtaa tatttcttca tattttggta ttttttactt ttattttttg
     9301 ttttcattca ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc
     9361 aggtgctgat tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc
     9421 atagccttct tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata
     9481 gggcccgacg tgtaagattt gggcacaaag accttcttcc atctttttga atactacttg
     9541 ttctttcaag tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc
     9601 tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac
     9661 tggttcatgc aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata
     9721 catctgataa tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac
     9781 tgcatataga caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct
     9841 gatgctaaag aatgattttt taggaacctc tagtatttgc gggctttttt tcatactata
     9901 gctcttgtct ttacgccatt caattttttc catttttctt cctccgcgaa attccattta
     9961 gttatttatt atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc
    10021 agtttgtgct agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg
    10081 agagagaggc ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt
    10141 agaggtgatg taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac
    10201 agggatgatg tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac
    10261 tcttatcaga cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct
    10321 tttgatgatt tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa
    10381 aaaactcttt ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc
    10441 tcctaccttc atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc
    10501 tcttccgttg atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat
    10561 gatgggtgtc ggtactttga caacggccat tgctccagca attggcccca cttatggagg
    10621 cattatgact tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat
    10681 tctttctttg attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta
    10741 tttggattgg ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt
    10801 gagtacgttg ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt
    10861 ccttttttac tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc
    10921 aaataaaaca ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg
    10981 tgtatctttt gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc
    11041 tggattagcg atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa
    11101 aatgctggat caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat
    11161 tggttggcta gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg
    11221 tcatgtgttt tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat
    11281 gaatgcactg aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca
    11341 gttttctggc gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca
    11401 tacttcccat aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt
    11461 gttgctgttg cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa
    11521 agcgtagatt ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg
    11581 tttgtaaaag gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt
    11641 atgaccctag ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg
    11701 acaggacagg caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga
    11761 aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa
    11821 gatttccaac ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa
    11881 aaagatttag ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt
    11941 ggaaacatcg aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca
    12001 gcagtttcac tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat
    12061 cttggtcagc gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat
    12121 aagaagagcg attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta
    12181 attttgacac ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat
    12241 atacgtccgc aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac
    12301 tggcctgttg ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc
    12361 cttttaggtg ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct
    12421 tataagggat tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac
    12481 tggccatacg agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc
    12541 ggcgtactgc cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg
    12601 accacaggtc cttatatcgg ctatctatta gccaacttct taaatactgg tcattggcct
    12661 acggaagatt atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac
    12721 agctttttca agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg
    12781 ttattttagt tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag
    12841 agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc
    12901 agatttttat aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg
    12961 atgtcttcaa atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg
    13021 attgacaatg tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca
    13081 tagcaggctg tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga
    13141 ggatagacac ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt
    13201 gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct
    13261 aaaactgggt cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa
    13321 acagcatcga tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg
    13381 actgtccgcc catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt
    13441 gtacatgtca cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac
    13501 ggatagtctt gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt
    13561 cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt
    13621 gctaaggtaa tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc
    13681 tggtcattac tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt
    13741 cccaatgccc aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct
    13801 agcacaaccg ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca
    13861 tgtgtccatt cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt
    13921 tgataagtct ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa
    13981 ttgttctcaa atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg
    14041 ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt
    14101 aaaattttct cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt
    14161 gctgttcttc ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa
    14221 tctagttctt cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt
    14281 gctagttgat aagctgtctt cctatcaaaa acttcatgaa cattattacc gtaaaatgtc
    14341 gtttctacaa tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat
    14401 tcgagactct cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct
    14461 ttattataca ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta
    14521 tttttcacac aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc
    14581 ttttatcggc ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac
    14641 cataacgaga tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc
    14701 cgtttaagta tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt
    14761 atgaaccgcc tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg
    14821 ttgcagcagc tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat
    14881 gttcttgcgt tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg
    14941 ttgctgaaga atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga
    15001 tgtttgcgat cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt
    15061 tttgtgagat cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag
    15121 catttgtagt tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa
    15181 ttttcttaaa tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc
    15241 tattaacttt atcatttaga aatatttcat aggtaaaaga aacatgacat ttaatgacaa
    15301 aataacgaat tatttaatat ttgtaacatt taaacaaata tttttttgat acaaaaccat
    15361 aaaagccttg ttgtataagc aaagtaatcg tttttctatg tttttttatt tttgaatgaa
    15421 aaacgcagat tctgtaacat tcactgcaat aatccataaa aaaaagcaaa aaaatttttg
    15481 atttttatca aattctctta aaaaaatgta aaaaatacaa aaaaaccagt aattgttaca
    15541 acaattactg gacaaaaact ttgaataatg ttaactattt ttttatttta actttctccg
    15601 caataatttc tgggcatgtt ccaattcgtt tataacgact tcttctgttt ctttagctaa
    15661 tatttcatct aatacatcaa gccattttta cggttctttt gttcctagac gtccaatagc
    15721 ccaagctgct gttcctctga tgacagggcg gacatctgtt aaacattctg aaatctcagg
    15781 caatgccgtt cgatcaccta gattagctaa agcaatcaat gcgtttctct gcaaaggttt
    15841 cttcccacgc catgaaccag ctaaatgccc aaattgctgt ttgaagtctt tattagacat
    15901 cgaaagcatt ggctttaatt ttggatatat ttcgtctact tttggttcca tctcttcatg
    15961 aaaatgaaaa tcttttcctt gattgtaagg gcagactaac tggcagatat cacatccata
    16021 aatgacattt cgcatttttt tacgatattc ttccggcatc atcccttttg tctgagtttg
    16081 ataagacaaa catcgctggg cattcattct tccgtcacca agcaatgctt ttgtcggaca
    16141 agcagtcacg cagcgcatac agtcaccgca accaaattct cctggtttat caggctcaaa
    16201 tacaatatta gtaacgatct caccaagata gacaaatgaa ccgaactctt cagtgatcaa
    16261 caatccgttt cgtccaataa atcctaaacc agctcgctgt gctacagcga catcgatcag
    16321 ttctccagta tctacttgcg gagctagacg ccattcctct tctttttgcc atttttctgc
    16381 tcttgtttgg atgaattcga tcaatcggtt caaacggtct ctgagtatat catggtaatc
    16441 gatcccccaa gaagcacgag caaactgccc gcgtttttca tccctaggca cttcttcatg
    16501 gatcctagta ggatacgcaa gtgcaatgga aatgatcgat ttcggctgat caaatgtcat
    16561 ttctggataa agacgctcat cgatatttgg atgttcaaag ccagaagtat ggccagctgc
    16621 tttttgttct tccaacgatt cttttaatga atcaaaaggt tccgccgtgg tgaacccgat
    16681 tttgtcaatt cctaatcgtt tgctttcttg tatgatttct tcttttaatg tcatggtctt
    16741 cctcgcttgc tgttaacggc tgtataaccc ataatatagt ttttcttttt tcagctggac
    16801 aaactggatc tgctgatcgg caatcttctt ttcttccatc catttttttc ttgcagcaaa
    16861 tacttcccaa taatttgttg aaattaattt tcttttgtta tctaatgcat taaaaaaatt
    16921 caatgaaact ggttttgtac ttaacagttc atggagcctt acctcttccc atggcgaaaa
    16981 ctcagacaga tcgcgggaat acaatacttc ttcatagaga tttttttgct gtgtgtacgt
    17041 gaatgtttgc gggattttcc caggcctagc aatttctact agacgattat aaaacatatt
    17101 cattaatttg ctgttggcaa taaattgact tttataatag gccgactgga tctttttgat
    17161 tactccttct gctttgatga ctgaaaataa tgcaggccat gctttgacgg ttatttcgtc
    17221 gttttgttta actaatacgc caggtgtgaa gccgactgga gaaaggaaca caaaataacc
    17281 gataccggta ttttcatcta aaggctgaaa ttcatcaaac aaccatttgc ctatgtacag
    17341 ctcttcatct ggttctgttg tctcattttg atttgctatg aataatttag ctaattctgc
    17401 ttcaagaaat tgaaggtctt gatccttgtc ggtattttcc tgctggctga tcaatcgaaa
    17461 gacttcttct ttaatcgtat atttatcttt acgtgtaaaa ttcatagcca caccttcctt
    17521 ccttactcta actataacgg aatttcttca aaaaaggtat ctgtacgacg aaaaacagca
    17581 taattttttt tcatatctct tataaaaaat gcaaggtctg ctcctcaagc tagaccttgc
    17641 acttttcagc tttcttttgt ttgtgacaaa ccttcatgga tcgtttcaat attccatttc
    17701 atcatctgat agtaagaatc tccttgttct ccttcttttg ctatggaatc agtgaacagt
    17761 ttatcataga ttttcaaacc cgtttctttt gctacccgtt ccatgcttct tgagtctaca
    17821 ctggtttcca cgaataaaac cggtacttct tttgctctta tctgatcaat gatcgctttc
    17881 atttgatcag gcgtaccttg actttctgta ttgatctccc atatataagc tgctggcaaa
    17941 tcatatgctc gtgaaaaata cttgaatgcc ccttcacttg ttactaataa ttttttgttc
    18001 tcttcgatag aagcaaaact ttccttcgct tgtgtatcta attcttttag tttttccaca
    18061 tatgcttctg catttttttt atagagtgtc gcattttctg catctttttc agagaatata
    18121 cgtgcgattt cctccacata ttttattccg ttagataggt ctagccaagc atggggatct
    18181 gcttttgtat gttcttcacc gctagttaaa taaagaggtt ctacattttt gctaactgca
    18241 aaataatctt ttccttcttc ttttttagcc gtttccatca agttatcgaa ccagctgtta
    18301 cctgtttcaa gattcaaacc gttgtataaa ataacatctg catcgcttgc ctttttgatg
    18361 tcttctggta gtacttcata ttcatgcgga tctgttccga ctggtacgat actgtggata
    18421 tctatttgat ctgttccgac ctcttttgcc atgtccgcca agatcgaatt ggtagccacg
    18481 actgacaact tttctttact tccttcatcc gcttggtcag aagcctgctt tccgcaaccg
    18541 gctaatacca agaaactaac tgccaagatt aaaaagagtg atttttttgt tttcatctat
    18601 ctgcttcctt tctgaaataa aattcctttt gttggagaaa ataaaaaagc aaggataaaa
    18661 aatgcggtac atgctaacac gatcgtagct cctgatggcc aattgtaact ataactgaaa
    18721 aacacaccga caactgcact tagcataccg attcctgcag ctgtaatgat cattttcagt
    18781 aaatggttcg ttagcaagta agccgtggcg gctggtgtga tcaacatggc aatcaccaag
    18841 attgttccta ctgtctgtaa actgactaca gccactaatg tcaaaaagaa catcaaaaga
    18901 taatggatca gcgatgtgtt cagcccataa gcttttgcca ttgtcggatc gaatgatgtg
    18961 atcttcaatt ctttataaaa gaaaaagaca aatatcaaga cgattccact aacgagtgct
    19021 gtcagcaaca aatcgctttc cctaacagct aagacattcc caaataaaat atgatacaaa
    19081 tctgtcgcac tctgggcata tgaaatcaag ataatcccta atgcaaaaaa ggagctaaag
    19141 acaattccaa tcgctgtatc atttttcaat cggctccgct gagtcacgaa accaatcact
    19201 gctgctgtta gcatcccaaa agcagtggct ccaaagatgt aactaaaacc aaacatataa
    19261 gaaatggcta cacctggaag aactgcgtga gagatagcgt ctcccatcaa agacatccct
    19321 cgtaaaataa taaaagaacc gatgactcca gaagcaagtc ctacaagcat ggaagtgacc
    19381 aaagcgtatt gcaggaattg ataattcatt aatccatcaa taaaggattg gatcattttt
    19441 tctctcctcc ttttactaaa tgtctgatga tctcaccata ggccaattga aggttcttcg
    19501 atgtaaagac ttcttgtact ggaccggaag cgatcagctg tttatttaaa agaataacct
    19561 catcgaaata ttcttctact ttatgaagat catgatggac gatcaaaatc gtttttcctg
    19621 aattcttcaa ttcctgcaag atgtcaaaga tctttcgttc acttaacgca tcaatcccta
    19681 cgaatggttc atctaaaaag atccattctg ctccttgggc tagagctctt gcaataaaaa
    19741 ctctctgaag ctgtccaccc gatagttcgc tgatctgtct tttggcatat tcttccatcc
    19801 ctactttttt caaagcttgt cttgcacgtt ctttttcttt tttcccaggt ctttgtccaa
    19861 ttcgtaaaga tggatatgtt cctaaaagta caacgcctaa tacatcaatt ggaaaggaaa
    19921 gatccaattc acttcgctgt tccacgtagg ctattttttt acggactgag tttaccgact
    19981 gtccagaaaa ggtgacttta ccagtagtag ctggtatcaa ctctagcaaa gatttcataa
    20041 atgttgattt tcctgcaccg tttggtccga taataccggt aattttttgt ggatttatcg
    20101 tcaagttgat agttttcaaa acagttttcc cttgataagc ggctgaaagg tcttgaatta
    20161 caatggccgt acgttccaaa tcttacactc ctttttaggt ttacctaact ttttatttaa
    20221 atatagttta actaaaatgg aaagcatttt caagtagaat gatgaaaaag tttttttcga
    20281 attttcctcg cattttatga aaattttctg tagaatcaca tcaagagttc aataaggcta
    20341 acctaataat gaaaaacatg tgttgaaacg tttgattatt ttttcttgtt gactcaacaa
    20401 cgaaaggagt ttttgtatgg aagaagaaaa gttaactcga agtcttagtg cccgtcatat
    20461 ccaaatgatc gcactaggtg gaacgatcgg tgtcggtctg tttatgggtg cctcctcaac
    20521 catacgttgg acagggccct cagtgatggg agcctatgcg attgcaggat tctttttgta
    20581 tttgattatg cgtgctttag gcgaaatgct ttacgttgat ccttcgactg gttcttttgc
    20641 aaattacgcg agcgagtaca tccatcctgt ggctggctac ttgaccgctt ggagtaatat
    20701 ttttcaatat attgtcgtag gaataagtga agtgattgct gtaggatctt atatgaatta
    20761 ttggtggcct gatttgccgg caattattcc cggaatcatt gtcgtcttgt ttcttatgct
    20821 ggcaaatctt atttctgtca aggcgtttgg cgaattagaa ttttggtttt cgatcatcaa
    20881 agtcataacg atcattttga tgatcattgc aggtcttggg gtaatcttat ttggatttgg
    20941 taatcatggt caggcagtag ggatttcaaa tctttggaaa aatggtggat tcttcactgg
    21001 aggcgtcaaa ggatttttct ttgcattatc gatcgttgtt gcttcctatc aaggaatcga
    21061 actgatcgga atgactgctg gtgaagcaga gaatcctaaa aagacgatca ttgaagctgt
    21121 ccagtccact atcggtcgaa tcttgatttt ttatattggt gctattttta tcattgtgag
    21181 tatctatccg tggaatcaat tgagtgagat tggttcacct tttgtacaaa ctttttctaa
    21241 agtcggcatt acatttgctg caggactgat taattttgtc gtgattacag cggcattatc
    21301 aggttgcaat tcagggattt tcagcgcaag tcgtatggtc tatacacttg ccataaatgg
    21361 aaagatgtct aaaaaatttc tgaaactatc tcgtcacggt gcgccttttt atcctgtcgt
    21421 agcaatctca ggtggaatct taattggtct gatcttaaat tatcttctac caatgttcat
    21481 caaccagtca gaaaatattt tcgttttcgt atacagctca agtattttgc ctggtatggt
    21541 tccttgggta gtgattttga tcagcgagat tcgcttcaaa aagaagcacg cagataaaat
    21601 gcatgatcat ccttttaaaa tgccactatc tccttatacg aattatgtga cacttgcttt
    21661 tttagctatc gtattgattt tcatgtttat caatcctgaa acgagaatct cattgattat
    21721 tggactcgtt ttcttgcttt acatgacgat ccattacttt gtacgcgaaa aaagaaccgg
    21781 aaaataaaaa aagaacgaag cttcacctta gtggaagctt cgttcttttt tattgctttt
    21841 atcaattcaa ttttagcgtc tcaccgggag tcaggaaata attctccatg ctcatcccat
    21901 tcagcgacaa gatcgtttct actggtacgc cagttcgttc ggctatttga tttggacctt
    21961 ctccttccaa aactacggtt gtcgcagcct gtgaagtcgc gctgttggta ttagtttcag
    22021 aggaatctgt tgtgtcttgt gttgttcgag cttgcatttc agaagcttct accgactctg
    22081 ctgtctcaga atcagaagat gttgtgtctt gtgtgcttgt tgactctgct tgaagagagg
    22141 cgctagtctg caaataaata tcggaaatct tagcgacatg attcgtataa ttagccgata
    22201 attgattgat caaagtatct aagtctacac cgtctttatt ttctgctgcc tgatacgctg
    22261 cacgtaattt tctgatccct tcgtcggaga ttgcttgaag ctcacgtgct ttttggttag
    22321 caactgctga taaaccagct attccctctt gatttccctg aatttctgtc tggtattctt
    22381 caatcaatgc aggcgtcttt tcttcgatct ttttcgaata atcatttacg attgcttgaa
    22441 gttgctctgg caaaccattt gctgtttttt cttcagaagt ttgtgtctct tttgttttag
    22501 ttgatgactc acttgtttgt ttcgtctctg ttttctttgt cgttgtttcc gttatggtcg
    22561 tacttttcgc ttctgacgaa ttttttccat gaaaatatag acccgcaact cctcctgcta
    22621 tgatcaaaaa cacggcaact gctgccccaa tcatgatcat ccgcttacga gctgcctgtt
    22681 tttgctgtct tcttcgggtc atccgaacat tttctcccca ctcattttca tcatacattt
    22741 ttttcaccac caatttttat gtataatagc gttatcaaac gcttcatatt cttattgtag
    22801 caaataattg tcgctgtgca catttttatc tttgttattt ataagaaaat ttcaccagtt
    22861 gagatgattc caagattgtc cgtcgatttc tccttgcagc tgcattgttc gttcgtctat
    22921 ctcagccatc agtttcttta tttcatataa gagcgcacgg tcttgatatt ctgatttttc
    22981 gcttaattca tctaattgtt ggatcagcca atcatttgtc ataatgaagt tcctttcatt
    23041 tcttgcagtg taaaggtcaa gtcttcgatc tgctcttttg cctcagtcag tgcttctacg
    23101 atatatgctt gtttttcaga ttcttcttcc tgcattttct gattttcctg tactctttga
    23161 ataaaccagt caatccgctg agtcagcgat ggtttgcctt ccaatgagag atactgctga
    23221 taagcaatta gcttctccca acgtttttcc tgggataaat acataaattg tgctaaaacg
    23281 aatggcggaa gataggtcat atggaattta tttgcgattc gctgatacgg cctcaaaaat
    23341 tcggaaatcg tatattcttc ttttcctcca gcttggtatt ctttttccgc cacacctgtt
    23401 gtcaatacga ttcctagttc tttccctttt aatacatgtg ctgctcgttc caagacatta
    23461 tcttgccaaa tttttaaatg agcaggagaa ctataccagt aaaacggaaa ctggaaaatg
    23521 atccgatcat gctgggccaa gagctgtagt tcagtctcta tatctatttt tccgtctgga
    23581 taacttgatt ccaaatgatg ccaagtagct ggtaaaccag cagaagcttc tttgaaaaat
    23641 tgctgattta aagattcttc gatcgttgga tggctaatga tgataagtgt acgcatccct
    23701 tttcctcctt tttagtcttc gctttacagt gtagcataac cgccacaagt ttcttttgcc
    23761 gatgcttgtc attttaacca gactaatcag gttgtccaag aaactgcggt ttgtttttcc
    23821 aactgttttt tcaaggtatc caaagcaata ttcttaaaat ttttgttttt catacgttta
    23881 cgatagttcg tagccgtttc tccggtactc tttttgaaca ttcggctaaa tacgactaga
    23941 ctattgaacc ctacttgata ggcaatactt tccacggatt catccgtcag agaaagaaga
    24001 aacgcccctc gattgatccg taccccgatc aaatattcct tgatactggt acccatatga
    24061 tttttgaata ctcgcgacaa atggctccga ttgaccgcta aatgctgggc gatttcctca
    24121 acagacaatt tttcagcata atgtgcctcg atgtatttga ctgtttcaac agctaaagag
    24181 gaatgaacct tttgtacacc accgctgacc tttccgccat cttttaaaag gactgataaa
    24241 aaacggtagg ttaatgctgt taactgtaat tcattttcca aggtttcttc cgtatctttc
    24301 atgcattcta aaataatgtc gatatatttt tgtatgtcag aagagaccat cgtgtattgg
    24361 tcttctctga aaagcgactg ccgaatgatt tcttctgcag cactcccacc aaatgaaagc
    24421 cagcaataca tccacggatc ttctccatct gataagtaaa acgtcgagtc tcctggacga
    24481 atcaagaaca gatcaccttt tttcaattgg aattgttgat ttttcacatg ataagatcct
    24541 ttgccttcca tcacgacatg caggatgtaa tcattgcgaa tagcaggacc aaaagaatga
    24601 aacggcatcg ttttagagaa accacaaaaa tcaaaataca attctttttc tacttgttga
    24661 tgttcctttt ttaaaaaaat cgattgctcc acgtctatcc ctccttactc ctttagtata
    24721 atgcaacaaa aagttaaata aaagtcacag aaatatatta aaaaggaagc gatttcttgt
    24781 taagtttaag tcattaaaag atctttgtta ttcgttgtaa ccggtttact tagtattata
    24841 tattgaacag gagaggaata cagattatga aaagcaaaat gatccaacgt gtgtcctacg
    24901 ctttaggcgc ttttggacac gacacattct atgttacatt aagcacttat tttatggtat
    24961 tcgtgacaag ccagatgttc gcaggaacag ataaggcaac gaatgcaaaa atgattgcta
    25021 cagttacaag cttagttgta ggtatccgct tagttgaaat tatttttgat cctttgattg
    25081 gtggcatcat cgataatact cgtacaaaat acggtaaatt caaaccatgg ttagttatcg
    25141 gcggcgttgt tagttccgta ctcttggtag caatctttac caatttcttt ggcttagcta
    25201 ctcataatac gactttattt atcatattat ttgcaatcgt tttcatctta ttggatagtt
    25261 tttattcatt taaggatatc gctttttggt caatgattcc agctttgagt acggaaacaa
    25321 aggaacgtga aaaatttgct acgtttgctc gtttcggctc ttctctaggt gcaaatggta
    25381 ctactctagt cgttgtccct atcgttactt tctttaccta tcttgttact cataaacata
    25441 ttgaaggcgc ttcaggctgg ttctggttcg gtgtgattgc tgctgttatt tcaggcggta
    25501 ctgcgattat cactgccttg ggcacaaaag aagtcgatac tgcgatccgt cagcaaaatg
    25561 aaaaagctgg gatcaaagat gtgttcaaag cagttgctca aaatgatcag ttgatgtggc
    25621 ttgcccttgc ttatatcgct tatgcgattg ccaatgcagc aacaactgcc gtattattct
    25681 acttcttcaa atatgtactt ggccaaccaa ctgaattttg gatcgttggt ctagttgctg
    25741 ccttacttgg tttagtagct gttccactat tcccaatgct tacacgcgtc atctctcgtc
    25801 gctacgttta ccttggaggg atcgcaatga tgatttcttc ttactttttc tttacgatcg
    25861 caggttcaag tttgccaatc gttatcatcg ggctagtatt gttctacttc cctcaacaat
    25921 tgattttctt atccgctttg atgacgatca cagactctgt tgaatatggt caatggaaaa
    25981 atggtatccg taacgaagct gttactctat caattcgccc tttactagat aaaatcgctg
    26041 gtgctttgtc taacgggatc gtagggtttg tcgctatcgc ttctgggatg actggtaatg
    26101 ctactgctgc tgatatcact gctcatggcg taacgacttt caaagcttat gcattctatg
    26161 ctcctgctgc tttgatgatt ttctccgcta ttatttttgc ttggaagatt acattgacag
    26221 agaaaaaaca tgcacaaatc gttgagaaat tggaaaacaa attggctcca gctgatacaa
    26281 tggaagatga acttcacgat gcagtagttc cttcccatta agaaaggatc ttgaaaatga
    26341 agattacaga aaaagatttt ggccaaggtt atcacttgat aaccttggag aacaaaaaca
    26401 agctagcact ttctatctct gatttaggtg ctagaatcgt cagtttaaaa tcaaatgatc
    26461 gagaattagt tttgggattt gacaccgctg aagaatatat cgaaaaagat ccgtatatcg
    26521 gtgcttctat cggtcgcacc gcagggagaa tcgaaaatgg ccacttttct ctcaatggaa
    26581 aaacttacca attagccaca gatccaaaaa ctggacataa tctgcatggt ggtgctcctg
    26641 gatttgaatt aaaaaaatgg tcttatgtca ttttgaatgg cgaaaatgaa gcttctgtca
    26701 tttttactac tacaagtccc gacggagaac atggttttcc aggtacaatg gatgtggaaa
    26761 tccgctatac tttgacaaaa gataatatct ggcgagtgac ctctcgcggc accagtgacc
    26821 aagatacttt attcaatccg acaaatcatg tttacttcaa cttgacggga gatgcttctc
    26881 agtcaatcga tcaacatgag ctttggctca atagcgaagc ttatgctcct ttgcgaacag
    26941 atagtattcc tataggggta aaagaaaatg ctgcaggaag tgctttcgat tttcaaatac
    27001 ctaaaaaatt agcgtccgtt tttgctagtg atttggatca aaaaaatctt gttgacggaa
    27061 tcgatcatcc atttttctta aaagaaaccg gcttgggtaa agaagccgct cgcttgacta
    27121 gccccgataa aagaatccaa gtagatattg ctacagatgc ttctagtgtg gttattttca
    27181 ctgcaaattt cggcacagaa acaccagaaa tgcgcaaccg aaaactggca catcacggtg
    27241 gcatcacatt tgagactcaa acagctccag gtgcagagcg cttctcagca tttggttcga
    27301 ttcatttaaa agccggatca gtatttgaaa cagtgacaga attcaaaatc aaaacaagaa
    27361 aggaataact catcatggaa atcaaaagaa gcttaaacac aaaatttgcc gaaacattcg
    27421 gacctaaaaa aactacacaa tacttctcac caggacgcat caatttgatt ggtgaacaca
    27481 cagattacaa tggaggccat gtcttccccg cttccatcac ttatggaacg caaggtgtag
    27541 cagcacctcg agacgataat aaaatattag tttattccac aaactttgac gatatcggtg
    27601 tcattagttt tactttagat gagctagaat atgataaaaa agcaaactgg gcaaattacg
    27661 taaaaggaat ggtccttaaa ttaaaagaag ctggacactc aatcgatcat gggtttgagt
    27721 tattagtgga aggaacaatc cctaatggtg caggactttc cagtagcgct tcattagaat
    27781 tattagtagg tgttgtatta gaagatctgt ttgatttaaa aacagatcgc ttggaactag
    27841 tccaaacagg taaaaaagta gaaaatgaat tcatcggtgt caattcaggt atcatggatc
    27901 agtttgccat cggcttcggt gagctagatc atgcaatctt attagataca aatacgctga
    27961 aatacgaaat ggttccagtg aaactagatg gttatgcaat cgtcatcatg aatacgaata
    28021 agcggcggga attggcagat tcaaaataca atgaacgccg tagcgaatgc gaagaagcct
    28081 tggcacgttt gcaaaaaact ttgcctatct cttctttagg agacttagat gaagaagaat
    28141 ttgaatcaac tattgaccaa atcggcgatg atacattgat acgcagagct cgccatgccg
    28201 tttctgaaaa tcagcggact ttgaaggcaa aagctgaatt agaagctggg aacttagaag
    28261 catttggtca actgttaaat gattctcacc actctcttcg ctatgattat gaagtaactg
    28321 ggatcgaatt agatacacta gtagatgctg ctcaaaaaca agaaggtgtc ttaggtgcac
    28381 gaatgacagg agctggattt ggtggctgtg cgattgctct tgtcaaagaa gaaaatatac
    28441 ctgccttcaa aaacaatgtc tatgatgaat acatgaaagt tgtcggctat gctccggaat
    28501 tttatgttgc acacattgga aatgggacaa caaagatcga tccagaagct gttcatgttt
    28561 gataaaaaaa tcaacgatct cagctaaaaa caaatagata aaaataacct ctcagcaatt
    28621 tccaaatgtg agccttttgc tcgtatttga aaattgctgt tttttattga cacggaaaga
    28681 gaacgtgtgt tctaattgtg ttgaagaggt gattgttatg tgggcgaatc aaaatccggt
    28741 atttgtttat gaaaaagaac cttcaagaga tattttatgt attgactgca aatcttttta
    28801 cgcctccgta gagtgcgttg aaaaggatct tgatcccctt acaactaaat tagtagtcat
    28861 gtcctatccg tccgattcga cagaaacacg gggtagcgga ttaatcttgg cttccagtcc
    28921 tgcagcaaaa aaagcttacg gcatcaccaa tatcagccgt gccagagatc ttcccttccc
    28981 ctatccaagt gatttatata ttgttgcccc aaggatggcc tattatatgg aaaagaacag
    29041 agaaatcaat tctatctata aaaaatatgc agacgaagcg aaccatcatg tctattccgt
    29101 cgatgaaagc ttccttgatg taacaaatag catggcactc tttggtaaaa caaatgcgta
    29161 cgacatggct aaactgatcc ataaagatgt gtttgagcag accggaatct acacgactat
    29221 aggaattggc gataacccgc tacttgctaa actagcctta gacaatggct caaaagacgg
    29281 aaagcaaatg tttgctgaat ggcgttatga agatgtacca gaaaagttat ggaaaatctc
    29341 agatatcact gacttttggg gaatcggcaa acgaacagca aaacgtctga aacaaatggg
    29401 tatacattct gtttatgatt tagctcacgc ggattattat cacttgaaaa gcaagctagg
    29461 ggtcatcggt actcagcttt atgcccatgc ttggggcatc gatcgaagtt ttttgggaca
    29521 agaatataca ccaaaatcaa aaagtatcgg aaatagtcaa atcctagata gagattatac
    29581 aagacgagaa gaaatcgaga tcgttatcaa agaaatggcc gaccaagtcg gcactcgctt
    29641 gagacgatct gcagcaaaaa cacaagtagt cagcttatgg gtaggatact ctttaggcta
    29701 tcttgatcca aatggcaaga ccgggtttca ccagcaaatg aaaatcgaag cgacaaatgc
    29761 tagcagagat ctagcagatg ctttattgac cattttccac cagcattacc actaccagga
    29821 tattcggcgt gtaggcgtga actgttccga cctcgtatac gcgacaggtt tacagcttaa
    29881 cctttttgaa gatcctgaaa agcagataaa tgaagcgaaa gttgattttg ttgtcgatac
    29941 gattagaaaa aaatacggct ttcaatcaat catccatgcg catagtctct tgcaaggagg
    30001 acgcgccatc gcacgtagtt ctctcgttgg cgggcatgca ggtggtatga gtggattaga
    30061 aggtggaaaa gatgagacgg acagcaaaaa aattcattga ctataacgag tatcgagatc
    30121 ggccatttgg attaaaatgg gggactgctt ttgcgatgga agaactagtc aacggtatca
    30181 aaaaaaatga agaagaagca cgcaaacagc ctcttcctaa acaacaaatg acattgcaag
    30241 aaatcgatag tatactgaca gaagcctttt tatctcgtaa agcagtaatc attcagctca
    30301 atttaaaaga tgaatttgga agactcttgg atgaggtcga agggcacttc attggtgaag
    30361 cctatgaaga ttactttatg attgaccatc agccgatatt gtgggaagac gtacggtata
    30421 ttgagttaaa ggaaacaaaa aaatggtttg tgacatgaga ggttgtgaca gaagtgatca
    30481 acttcaaaaa ataagaaagg atttttcgaa aattgcttct taaatttttg tggaattttg
    30541 gcttattttt cgaagaagca acttctggaa caacgtatat tagatatcag aggttgtgaa
    30601 aaagtcgctt agttttgagt aacaagccaa ggcgctgatt tcacaacctc tgtctatcta
    30661 tccatctatt cggtaagatt catgcatgac gtcttcttta tttagtatgt agagcccttt
    30721 ttcttctgac cattcaacat aaatcagctg ttttacatct gcatagcctt ctttcttcaa
    30781 gttctccata agccatgctt cagtcaattg attggactga agcactttat gctgaatttg
    30841 cccttcatct acaagcagga tcgataaagc tttgtcttca tctgattttt tcaatacact
    30901 catttgaccg gcattctcta ggataacatg tttggcattt tcaagaaaaa agcattcttg
    30961 ctgtcttaag attgctcgca actgctccat ctcgatatgg ttatttgtca actctgtcat
    31021 gttgatgacg ccgtctttga ttagaacaga tggctcgcct ttgacccaac gattgacctt
    31081 ttcatttttt tggacgatac gttcgatacc ataaatcatt actgcccaaa gcgcaatggc
    31141 aaataggaca tgccctacat ggacattgtc atcataggtt gattcttcta agatcccacc
    31201 taaaacaagc gtatagacta aatcaaacgg tgtgatttct gacatggatt tcttcccaag
    31261 caaacgtacc actaataata aaccgatcaa cccgaacacc agtttcattg taattgaaaa
    31321 aatcattttt tctcccccta actatctgtt aattaaatta tatatgcttt ctcacgaaca
    31381 cgcatggaat atacaaggaa gaaatgtatc cttttactaa aaaaaggact gtgacactgt
    31441 agtgaccccc aaaagttaga cttttttgga gtggagaaat ccactccttt tttttatgct
    31501 ctaaaatgat atttttacac aaccgttacg gttgttttac gaaattggac g
//
LOCUS       DACEUF010000026        31249 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.027, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000026 DACEUF010000000
VERSION     DACEUF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.027"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(56..1285)
                     /gene="rpsA"
                     /locus_tag="IXG20_001157"
     CDS             complement(56..1285)
                     /gene="rpsA"
                     /locus_tag="IXG20_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ1609366.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(1420..2100)
                     /locus_tag="IXG20_001158"
     CDS             complement(1420..2100)
                     /locus_tag="IXG20_001158"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ1609367.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTREVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(2149..2784)
                     /locus_tag="IXG20_001159"
     CDS             complement(2149..2784)
                     /locus_tag="IXG20_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1609368.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(2850..4253)
                     /locus_tag="IXG20_001160"
     CDS             complement(2850..4253)
                     /locus_tag="IXG20_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ1609369.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(4240..5271)
                     /locus_tag="IXG20_001161"
     CDS             complement(4240..5271)
                     /locus_tag="IXG20_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609370.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            5302..5523
                     /locus_tag="IXG20_001162"
     CDS             5302..5523
                     /locus_tag="IXG20_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ1609371.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(5688..6425)
                     /locus_tag="IXG20_001163"
     CDS             complement(5688..6425)
                     /locus_tag="IXG20_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ1609372.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(6578..7918)
                     /locus_tag="IXG20_001164"
     CDS             complement(6578..7918)
                     /locus_tag="IXG20_001164"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ1609373.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(8155..9678)
                     /locus_tag="IXG20_001165"
     CDS             complement(8155..9678)
                     /locus_tag="IXG20_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ1609374.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(10056..10667)
                     /locus_tag="IXG20_001166"
     CDS             complement(10056..10667)
                     /locus_tag="IXG20_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1609375.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(10790..10915)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(10997..11713)
                     /locus_tag="IXG20_001167"
     CDS             complement(10997..11713)
                     /locus_tag="IXG20_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1609376.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(11719..12288)
                     /gene="scpB"
                     /locus_tag="IXG20_001168"
     CDS             complement(11719..12288)
                     /gene="scpB"
                     /locus_tag="IXG20_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ1609377.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(12272..13069)
                     /locus_tag="IXG20_001169"
     CDS             complement(12272..13069)
                     /locus_tag="IXG20_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ1609378.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(13039..13410)
                     /locus_tag="IXG20_001170"
     CDS             complement(13039..13410)
                     /locus_tag="IXG20_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1609379.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(13429..14316)
                     /gene="xerD"
                     /locus_tag="IXG20_001171"
     CDS             complement(13429..14316)
                     /gene="xerD"
                     /locus_tag="IXG20_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ1609380.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(14412..14885)
                     /locus_tag="IXG20_001172"
     CDS             complement(14412..14885)
                     /locus_tag="IXG20_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1609381.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(14990..15853)
                     /locus_tag="IXG20_001173"
     CDS             complement(14990..15853)
                     /locus_tag="IXG20_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1609382.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(16049..18148)
                     /locus_tag="IXG20_001174"
     CDS             complement(16049..18148)
                     /locus_tag="IXG20_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1609383.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(18368..19477)
                     /gene="rpoD"
                     /locus_tag="IXG20_001175"
     CDS             complement(18368..19477)
                     /gene="rpoD"
                     /locus_tag="IXG20_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ1609384.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(19501..21342)
                     /locus_tag="IXG20_001176"
     CDS             complement(19501..21342)
                     /locus_tag="IXG20_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ1609385.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(21524..23917)
                     /locus_tag="IXG20_001177"
     CDS             complement(21524..23917)
                     /locus_tag="IXG20_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1609386.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(24024..24884)
                     /locus_tag="IXG20_001178"
     CDS             complement(24024..24884)
                     /locus_tag="IXG20_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ1609387.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(24943..25575)
                     /locus_tag="IXG20_001179"
     CDS             complement(24943..25575)
                     /locus_tag="IXG20_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ1609388.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            25784..26503
                     /locus_tag="IXG20_001180"
     CDS             25784..26503
                     /locus_tag="IXG20_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1609389.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            26529..29120
                     /gene="mprF"
                     /locus_tag="IXG20_001181"
     CDS             26529..29120
                     /gene="mprF"
                     /locus_tag="IXG20_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1609390.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(29308..30495)
                     /gene="tuf"
                     /locus_tag="IXG20_001182"
     CDS             complement(29308..30495)
                     /gene="tuf"
                     /locus_tag="IXG20_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1609391.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            30863..31219
                     /locus_tag="IXG20_001183"
     CDS             30863..31219
                     /locus_tag="IXG20_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1609392.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         8663 a         6612 c         5394 g        10580 t
ORIGIN      
        1 agaaaaactg agacaaaagc agctgctttc atctcagtca cagatacaaa atcaattatt
       61 tttcagaatc atcggttgct ttttcttcta cttcttcttt caacgcatca cctaaaatgt
      121 ctcccatagt aaatccagta ttttcttctg gtaattcata agactcaaca ttttttggtt
      181 cttcttctga agctggtttt tcttctaaag ctttgatact caatgcaata cgatgttctt
      241 ctggatggat ctctaatact ttcactttca cttcgtcacc ttcatgaagc acttcatgtg
      301 gtgtcgcaat atgtttatgt gaaatttgag agatatggac tagcccttct actccaggga
      361 acacttcaac aaaggcaccg aagcttgtca aacgtttaac tgttccgtct aatactgtgc
      421 caactggagc ttttgtatcg atttctgacc aaggtccagg ctgtgtgtct ttgattgaca
      481 aggaaacacg ttcttgttca ggattgatag acaacacttt gacatttaca tcatcattga
      541 cagccaatac atcacttggt tttgctacat gactatgtga aatctctgag acatggacta
      601 gaccgtcaat tccgcctagg tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc
      661 cactgagaac atctccctca tggatagttg aaagaatttc ttttttcttt tcttctcttt
      721 cgttttccac taccgcttta tgcgataaga tcaaacgatt ttctgatggt tcgatttcga
      781 tgattttaaa gctcaatgtt ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt
      841 ccaccattga tgcagggaca aaaccacgta caccgacgtc aacgactaaa ccgcctttga
      901 caacatttgt aacaggtgct tcgatgacct tgccctcttg gaaatctctt tcgatatctt
      961 cccaaacttt tttagcatct aaacggcgtt ttgaaagtag atagctgccg ttttctttat
     1021 ctttgccgat cgtactgata acgactaaat ctaatacatc accgactttt actacttcgc
     1081 tgatatcttc taccggcaaa gttgataatt cttttgccgg aacgactcct tcaactccag
     1141 ccccttcgat tccgacaatc acttgtttgt cttcaatgac taaaacttcg cccttaacaa
     1201 catcgccaac ttttacttct tgtacgcttt tcattgcgtc ttccattgac tggttgttct
     1261 cttgattttg atcaaattct gtcataacac ttgtcctcct aaccaacatt ctgccgtaag
     1321 tacggtactg tttttagttt aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt
     1381 tccttgaaaa aacaattgtt tttcattttt tttcttaatt taaaaaagaa gataaccttt
     1441 tttcaaaaca acagctttta ttgctgcaac gacttctgga atcgatttgc cagtcgtatc
     1501 gatacgaaca gcatcctctg cttgcttcag cggagagact tcccttgttg aatcataatg
     1561 gtctctttcc tgtatctctt tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg
     1621 gttctctttg aaacgtcttt ctgcacgctc acttacgctt gctactaaaa agattttcac
     1681 ttctgcattc ggtaatacag ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg
     1741 ttcccctatt tttcgttgga cagcaactaa ttcttctctg acttttccat gtgcagagac
     1801 ttcagaaaca gcttttgtga catctggttg acggatttct aaagtcacat cttttgatgc
     1861 tatgaataca tgctgtccat cttcttgttg cgaaaaagag ataggatact ttttgatcag
     1921 cgaaactagc ccttcttcat ctgaaaaaga tacattgttc gtaatagcta aataagttac
     1981 tgcgcgatac atggcaccgg tatctgtata aatataattc aattcttttg ctaatatctt
     2041 ggcaacggta cttttcccag atgaagcagg cccgtcgatt gcaatactga tttgtgtcat
     2101 gatataactc ctttttctca gaaaaaaagc cgcaaagcgc ggctattatt atttaattct
     2161 taattcttga ccagggtaaa gcataaagtt gtttggatct aagccattca attcaaacaa
     2221 ttgatcagtg gtgatccctg cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac
     2281 agtcgttgtt gaaccggctg cagctgatga agaaggtgtt gtttctgctg cattgctgct
     2341 tgaatcccct gcttgataag ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat
     2401 actgctttct gacgttacac ctgtatcttg agacacagta gattcttccg cgattgtact
     2461 agattctact gttgaagtct cagttgactc ctcttttgtt gtacttgtag aactttctac
     2521 tactgatgtc gaagtttcag aagaagttgc tgctgttgca ttgttgcttg atccatttct
     2581 gatccagaaa taagcacctg ctggaatagc gatacataag accagtaaaa tcaaaaatag
     2641 cgaaagaaat aatgtatttc ctttcttttg ttgtctctga gaacttcttg atgaaacttc
     2701 atcatctgta tcgtagattg gttgttccca tggttcttgt gtttcgttat tttctgtaga
     2761 ttgatgtcta tcttttctgc tcactgtttt acctcctaaa ctctatcgtg tgtattatac
     2821 cataggaaaa agtgtcttgt cctaaaaaat caatgttcca tgtttttttt aaataaattt
     2881 agtaaaatcg tttcccaatg aagttgttca gtcttagaaa aaagcttcct ctttggcaga
     2941 agttctgctt ttgctccatc aatatcacaa caattctgcg gcttttcgga gagtttttcg
     3001 ccaaaataag ctaagatgaa ttttctgcgg cagttttctt cattaatata gcgaagcatc
     3061 tgctgcaggc ggaattcttt ttctttttcc tgacgtttga gccgttccac ccaattttct
     3121 ggtttttcag actgctggat cagctccatc catttttttt gcagctcatc aaagggagct
     3181 tgctctgtgg catattccaa ataaagctca aaactttgtc gctgttctct agaaagttga
     3241 ttaaagaaat aatggatacg ttcatcccca ctttgataaa aaagaattgc tgcactttct
     3301 tcctgatctc tgcctgctcg accgatttct tgtacatagt tttctagact gtctggcaaa
     3361 tcataatgga ccacataacg gatatcagat ttatcgatcc ccataccaaa tgcattcgta
     3421 gcaatgagaa actgcagctg attttttaca aactgctgtt gaagttgtct tctttgagct
     3481 gcatcaagac caccgtgata gtatccaact gtaaaacgtt ccctgaataa atgatacaat
     3541 cgttcgactt ctttttttgt tgcacaataa atgattgccg ctccttgtgc tctttccatg
     3601 aagtgttcaa gttcttgttc tttttgctgc gttttccgaa caaaaagcga gatattttgc
     3661 cgattgactg attgtctgat ctcttttggt ggtcgatcaa atagtacatg acgtatatct
     3721 cttgctacta aatctgttgc tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc
     3781 tttcggattt ttcctaactg ttgatattct ggacggaaat caactcccca ttgagacacg
     3841 caatgagcct catctactac atataacgca atattttgtt ttttcagatg ctctaaaaga
     3901 gaaggattag tcaacatttc gggactcaaa aacaaaaatt tatattgcga tagatgacgg
     3961 aggacgtatc ttctttcaga ttgcgaaagt aaactattga aagcagctac tcgtttctcc
     4021 cctcgttttt gtaattgcgt cacttgatct tccattagag aaagcagcgg tgacacgatg
     4081 atgaccagac cttccattaa ataccccgtc aattgatagc aaaggctttt tcccgttcct
     4141 gtaggcaaaa tagcgagagt atcttctcca tcaagcaacg cctgaataat ttcttcttgg
     4201 ccgggacgaa atgaatcaaa tccaaaatat ttttttaatt catttattaa cattctgtat
     4261 cgcttccttt ctcaatagat agatttgata caaacgaaat tctccataat ctaattgtcc
     4321 atctacattc cattcgctaa agcgccactc tcgcacggaa tccttatttg gttccaaacg
     4381 ttcgattgtt tctgcagaga gaatccactc gtatggaaaa tcatcaaaaa acaaggccca
     4441 ctcaataaga tgatctgtta ctgttcctct ttttaaatga cggattgcca tgatctggtc
     4501 aattgtctga ccattcataa acatctgctt ggtgatcatc atgctttgat tgaaattttg
     4561 ctgtaataat gaatcgatca aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc
     4621 taacaaaagg tggatacatc tgctttgttg cagataaacc gctgactcat cattgttttt
     4681 tgccagctga taaggaagca gccctgttcg ctcatttcca gataattggt tagctaaaaa
     4741 atctgctgct tcttctggta tctccgaaaa cagctgcacc aaactctcat acgcagtatc
     4801 aatcaaaatt tcacgtggca attggctttc actaagccat ttttttagtt ggaaagtata
     4861 aaaaggactt gtctctgctg gaagatagtc ttgaataccc gttgccagat tagaaattac
     4921 ttgaacagca aatttaatga gccgccaact agtttcccct gttctgccat agcggtcaaa
     4981 acgcaaacca gtatgttcta tttgatgttc ggacaacata tcagcccctg caggcgttag
     5041 ctttgcttca ttttcattta tttcgatcca accttgctgc gcaatttttt gcaaaatttg
     5101 ataaaactta tcctgcttca attctggaaa actgttatgg ataaacaaaa gttcgtgggt
     5161 aaatccataa ataagtactg aagtcgttcg ttttcccaca aggagttggt aaagagaact
     5221 aatccgcagc ttgtgtcctg atgaaaatag cgataaaata aaaacaatca tattattctc
     5281 cttaagttag gatggttaga aatgtcacta ctatgtaaac ttgtacctga acgctgtatt
     5341 gcatgcggtc tttgtcaaat cgaagcgcca gatattttcg attatgatga agaaggaatc
     5401 gttttatttg cccaagaacc agaggctcat caacagttcg tgatgccgga agaagaaaca
     5461 tatgtaaaaa aagcgtatca aaaatgtcca gttcgagcga ttttattaga aaataaaata
     5521 taaatataaa cgaaacttta aaaacgtaca ctaagttctc taaacttgaa aataagccgg
     5581 gatctgtttc tcttgttccg tgaaatcccg acttattttg aaagattcat ttgctatcta
     5641 tagttaattg actagttttt tcacggaccc tataggaccc atttatatta tttcaacttt
     5701 ttcagccagt tttccaatgt attgacgatc acagtttgct ggtctgcgtt agaaatttct
     5761 gcctctccat ctcctttttg ctcaccatag ctgccaaaac ctccatgatt tccgccattg
     5821 attgatacat actctgttct ttccggtaaa tactgcttag cttcttgata agcttcctga
     5881 ttcaatactt tatcttttgt cgccgtgatc gataaaacag gaacagctga ttctgtcaat
     5941 gttccttttt tgtcgggata acttgccatg aagaataccc cttttaaatg tttgtctgaa
     6001 tgattcttat tgacataacg acttgccatt acgccaccta atgaatggcc accgatcaca
     6061 taatcggaat ttggaaaatc ttctttgacc ttatccgctt tgtttccgcc catcactgct
     6121 agatcaagtg gcattttgac aatagcgact gaaaagccgt tttttgctaa atcttgcgcc
     6181 caaatactat aactttctgg ctctacgaga gcaccaggat aaaaaataag taatggagac
     6241 tttccatgat tctttttctc tccttcaaaa aataagtaat cttctttgtt ttttccttct
     6301 tttaccaaca cttgcgcttc tgacgatggt tcgtacgtat tttgctgtat atagtaataa
     6361 ccggctccac cgagtaatcc taaaatgatg actaagccaa ttagtattcg tctaaatatt
     6421 ttcactttct cccacctttt ctatctgagt gacccctttt tcttaatcaa gtatgattat
     6481 accttaaaaa aataagagag aaatgcaaac tttccagcat caaataaaaa aagacggaat
     6541 atccatttga atatccgccc tttttgtatg tttattttta tttagcacat aaacgataca
     6601 cagcttctgc ataaatttcc attgctttat acatactact taattcccat cgttcattcg
     6661 cctggtgcat aaagtcagga gtatcaggga acatcgcacc gaatgccaca cactggttca
     6721 tcgtacgagc aaaagtagcg ccgcctgata caaacggttc agtcatgtct ccagtaatat
     6781 cgcgatatgt ccccaataga tttttcacta actcactgtc tagtggcacg tataatgaat
     6841 caaggaagtc aaattcttca taagtcaacc catatttttc tgctgtttta gttaatttag
     6901 ctactaaatc atctttcttg aatgtaacag ggattctcat atctacaccg atttttgttt
     6961 gttctggtgt gatttccaaa cttgcaaagt tcatcgttag ctcgccggat tgttcatctt
     7021 ctgttttgcc cacgacagct tcaccagttg cattttcttg aactaatttt cctaagaaat
     7081 tgattgggcc aaaatcaaat acttctgaaa gagcaattgc cagacgagaa atagcattca
     7141 ctccttgtgc tgcatctttc gcgtggatac ttttaccaag aacgacgatg ccttcacctt
     7201 gttcttcgaa atcgaattcg tgttttttca atgcttcttt tacttcagaa agcttttctc
     7261 cagagtaagg agctgcatca ggaacaacat tcaacgctcc tccagctttt acagaaaact
     7321 cgttcgttcc tggtcctgtc aagtaagctt gcaataagcc tttttctgca taaattaaag
     7381 gaaattcagc atctggcgca aatccttgcg taattccttc ttctttttcg ttatattttt
     7441 ctaaacaacg ccataaattt tcttcatctg taccaaagat aaaacgaata cgtgtcttga
     7501 attccacgcc agcatcaatc aaagctttga cggcatacat tgccgcaata gaaggacctt
     7561 tgtcatcttg ggatccgcga ccaactaacc agccgtcttt tatagttgga tcgaatggtt
     7621 ttgtttccca attattttcg tcccctgctg gaacaacgtc catatgacac aagataccga
     7681 ataattcatc tccagagcca atctctgaat aaccgtaata gccttctgga tctttaaatg
     7741 ttctaaatcc tagcttttca ctgatttcca atactttatc caaagcttca atcacttttt
     7801 taccgaaggg atgtccttgt cctgaatctg cttcatctag tacggaagga attcggatca
     7861 attcgctcaa tgattctaat gcttgttcat gatgttgttc tgtcacaaat tttttcatga
     7921 tattatcctc ctaatatgtt aaaaagggtc atgtcaaatt cctccgacac aacctcttct
     7981 tctcaaataa tgacgtttta agcctgctcc atcagcaagc agaatgttct taacaaatga
     8041 tgaaagaaaa aacactcact gaatggagct attgacttat ccacgtgctt atcgatagcc
     8101 gtttatcggt ttcttcattc ttatgataga ccttgtacct gtatttgtct aggtttagaa
     8161 cattgctgct acacctagga tcacacagat ggctacaaat aatatcgcaa ttaatttacc
     8221 ggtaaatttc caccacactg aaacatcgaa acgagcaatt gctacagcac ccattacgat
     8281 agcagatgtt ggtgtaatta agtttactaa accagatgca gattgataag cagtgattac
     8341 taaatcctta ccgacgccgg caaattcacc cataggtccc atgattccca ttgttgcagc
     8401 agctaagccg gaagttgaag gaatcaagaa tgacataggg atatagaaaa taaatgtaat
     8461 aatgatgaat acacttgaag ataatgaact taatccttgt tcgccccaat gtaagatggt
     8521 gtctgtgatt aatccattgt tcataaccac ttggatacca cgagcaacag ctactacgat
     8581 tgctacaccg atcaaatcag aagcaccagc taaaaattca gagacaaatt cagactcttt
     8641 catgccataa gtgattgcta ctacgatgga cattactaag aataacatag taatttctgg
     8701 gaagtaccag tcacctaatg gaagcatgct tttacctaga accgtaccta gtaccggaag
     8761 gtttgttaac cagtttgtca atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca
     8821 aggaatcaaa ctgatgatca tgataccaaa agtaataaag aacaatacat taacatgttt
     8881 ttgttttttc gtgattactt cttgacgacc gattgcttca atatcgaaat gcttcatatc
     8941 ttctttacgt tggttgtata ctaatgattt tgttggatct ttttcgatct ttgatgcata
     9001 acgatacaca tatacaatag aaatagctaa caaaataacg aacatcaaga atcgaagacc
     9061 aataccttca cccatcgaaa tcccaacagc ttgtgaagct acgcctgttg caaaaggatt
     9121 taccgttgaa gctagacagc ctacttgaga ccctactaat acgattgcta cagctactac
     9181 tgtatcaaag cctacagcca tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc
     9241 ttctgccatt ccgtaagtag agccgcctaa tgcaaataac agcatcaata ctggaataag
     9301 cattttttct tttcctttga atttcttcac aactgaagca atcccggcat ctagtgcccc
     9361 tgttttgttg ataataccta aaaatccacc aacaaccaaa atgaagaaag agattgggat
     9421 tgcagcaggt gtttctttag ttagctcccc cgctggtaca cctagcatcc cattgattgg
     9481 cgccatgaaa atatcccaca gcccttgagg attttgttgt actcgttcat atgatccagc
     9541 gacgatgttg cctgcatcgt ctacactata gtgcccagct ggtataaacc aagtaagtac
     9601 agcgattact gcaataataa taaataagac cgtatacgct gaaggcattt gaaatttttt
     9661 cttttttgtt tgttccatct tcttcttcct ttcttttccg ttagagacaa caattttggc
     9721 tctaatgtta ttttatcaaa aagagtatca gaacaaaaat aataacacgc attttgtaaa
     9781 acttttgttc aaaaagttcg tttttcccta ttccctcctc ttttttcagg tttttaaagc
     9841 tttcttcttt ttcattgtag cgtttccttt tttttataaa caaacatttt cgtcttttaa
     9901 aaggagaaga atttcacatt tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa
     9961 tactcaaaac aaacaaataa aacgtttaca ttattgcatg tcttgtattt ttttccaaaa
    10021 aaaaagacca agaaatttct tgatcttttt gatatttatt tgactgctgt atgacgttgt
    10081 tcgaactgat gttgttttcg acttaaccat ggaagaagtt gcgcatgcag cactaaaaag
    10141 acagcactca cgatgaatcc tttgatcaag ttgaaaggaa cgatcccaat cagtacatag
    10201 tttgctaaag acatgcccag catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga
    10261 ggcgtaataa cgaaataatt tgcgatactc ataaaaatag ttagagcgat ggttcctgta
    10321 atgacaccta aaaatttgtt tcttccttta tgctgtttct ttacactaaa gaaataataa
    10381 atcgggaatg taaagatcgt tgttgataag aaactagcag tgtctcctac caaattatcc
    10441 ggagaaaaac ctgtagtcag cagatgtaat actgaacgca agaacgctgt tgcaactccg
    10501 gccgctggac cgaataagaa catgctgatc aataccgaga tatcactaaa atcgatcttc
    10561 aaaaaactaa aagccggcat gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca
    10621 gcaaacatcg ctactgcaac cattttctgt acacgggtgt tcttcatttg aaatcctcct
    10681 gttaggactc ctcttcagcg aactttttga cgtttctctc caaatgaaaa aactctattt
    10741 tccagggaaa atagagttag agttatttga tcaagcgtcg tcaaataagc tctatcttct
    10801 ttatccagac tatactgtcg gtatcggaat ttcaccgatt cagctatttt tttgcaaaat
    10861 aggtcgtgga ctataaccac cggtcgggaa tttcaccctg cccctgaaga cgaaccttat
    10921 ataatttttg tttacatatt cattatacat atctattcaa aaaacgcaac atctaacact
    10981 tataaaaagt aagtgttcat tttgagtggt tcagcaaact gctgacttct tttttgttca
    11041 gttcacggta atccccagga cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt
    11101 ttagtttttg cacaggaaga ccaactgctt gaagcatatt tttcacttga tgattacgtc
    11161 cttcgtggat cgtcaattcc acgatactgt ggtttgtctt aggatccact gataaaattt
    11221 ggaatttagc tggagatgtt tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa
    11281 gcatcgattt tgtagcgatc ccttttacct ttgcaacgta aactttatcc acttcatgtt
    11341 ttggatgtgt caggcgctga gcaaaatctc catcatttgt cagtagcaag atccccgaag
    11401 tatcgtaatc cagtcgacct acaggataga tacgttcttt taccatcggc aaaagatccg
    11461 taacaacttt tcttcctttg tcatctgcaa cagcagaaat aactccttta ggtttgtaca
    11521 ataaatagta accgtattct tcttgataaa ttgggacttc atccacttcc acagtgtcat
    11581 gtttcccaac ttttatccct aattccctta caacttgtcc gtttactttt acacgtcctg
    11641 caaggatgaa ttcttctgct tttcgacgag aagtgatgcc agcgtgggca attactttct
    11701 gcaatctctc cattacaatt attctccttt attcattcca tcaaagaata gatctggtga
    11761 cacgtcttct tcaagtgaaa tttccatttc ttgtgtatca ggcagttctt ctaatgattt
    11821 caaaccaaaa taatctaaga agtagccagt tgttccatat aatatcggcc gacctggtcc
    11881 ttctactcta cctttttctt caatcagttt atgggcagct agacgctgta ccgctcctga
    11941 aacctgaact cctcgaatct gctcgatttc agctcttgtg attggctgtt tataagcgat
    12001 gattgctaag gtttcgacgg cggcttgaga taatgtgttt gatgtccctt gcgcatatcg
    12061 cttaagtaag ggagccagct cttttttagt cgttaataca taatggcttt ccgtttcaaa
    12121 aatatgcaaa gctgagtgcg agttttgagt gtaagattct tttaattctt tcactaaact
    12181 tgtgacttct gcttcgctga gatttaacag tccgcttaat tcttcattac tgatcccttc
    12241 ctcgccaaca ataaaaagaa gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc
    12301 ctactgctgg ataaagcata attgattcat agttttcttt ttgttgcgca atgatctgcc
    12361 cttttttcat caattctagc aaagccataa aagtagtgac aacttcactt ttagaaggtg
    12421 actcaaaaaa agaatcgaaa gtacagccat gcgcatttct ttttatacgc tcttcgataa
    12481 ataccattcg ttcttcgatc gttgtgtcat cgggcgtgat cgttttttcc actttttgag
    12541 aaaatttctt acgttctaag attttctgca tggcacggat caaatcgatt tttttgactt
    12601 gtcctcgctt caacggcttt tcagcatccg ctaacccatc gatgttcata ggttcttttg
    12661 tgtaataaag acttcgttcc tctgcttttt ctgtcagtct ttcagctgca tatttatatt
    12721 tacggtattc taaaagctga gtgaccaatt cttctcgagg atcttcttcc cataattctt
    12781 catcgtctgt gacgaattct tggacaggaa gcaatgtctt gcttttgatt gccatcaatg
    12841 tagcagccat cactaagtat tctcctgcaa cagctagctc gagtgttttc attgtatgga
    12901 tataattcat atattgctcc gtgatcgtag cgatcggtat atcatatata tcgatttcca
    12961 tttgctgaat cagatgaagc agcagatcga gaggaccctc gaatacatct aatttaatat
    13021 tgatctccgt caactcattc actctttctt ctttttcttt gctagccatt ttccaatgta
    13081 aggctctgtg atttcagttg cacggatgga gacatccggg tacttttcct gtaattcatc
    13141 taacatttga aagcccatat tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat
    13201 gatcagctga tcgttcgttg cccagtaaat accaataatc ccttgaatgt tggttgaacc
    13261 ttctggcgta aagaaaaaaa gttcataatc gtcttcagat tcgtacgtat caatctcctt
    13321 caacaaagaa gttttacctg tcagtttctt atggaaactc aatagtccca tagcaatttt
    13381 acggttttct ttccggtaat gtgttaacat cttctcacct tcctatgact aagcccttgg
    13441 aaaatgctgc ttataaacat ccgtcattct tttcttcgtg atatgtgtat aaatctgagt
    13501 cgtagagata tccgcgtgac ctaacaattc ctgtactgtc cgcaaatcag ctccattttc
    13561 tagcagatgt gtggcaaagc tatgccgcag cgtatgtggt gttacttctt tgtaaatccc
    13621 agcctctcgt actaactgct tcagattttt ccagattcct tgccgagaaa gacctttccc
    13681 atgattattg acaaatacat gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc
    13741 gtctaagtag cgttccagcc attggatcgc ataatcacct aaaggaatga tccgctcttt
    13801 atctcctttt ccaactgtct gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa
    13861 tccaatcaat tcactgacgc gcatcccagt agcatacatg acttccaaga ttgcacgatc
    13921 tcttatccct aacgtttttg tcgtatcagg tgcttcaatc agcttttcta cttctgtcag
    13981 agtcaatgta cttggtaatt tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc
    14041 atgatcagtg atacgctctt gccgtaaaaa ctgatggaat cttctgagac ttgagatcat
    14101 ccgagtaaca gttgctgtcg cttttttttc ttcatgcaac ttctccagaa aacggatgac
    14161 taaaaagcga tcgacatcct gccatgaagc aattttctgc tcctgtagaa aatgcaagta
    14221 ctgttccaaa tcacgttcat agctttgtcg tgtattggcg gacaatcccc gttcaatatt
    14281 caagtagtgg agataatcgt tgatttgctc tttcataata atccccctca tgcctctcta
    14341 tcatatcaga taccgcttca ttctgatagt ctttttccat aatctgagca aagaaaaagc
    14401 cgtctagaca ctcagacgga ctggtttctt ttttcttttt catagcattc ctgacagatt
    14461 ccatggaacg ttagccgatg atcttttact aaaaaatgat aacgttcttc tactacttgc
    14521 tccacttcaa gtaaaagatc ctcttctact tcctcgatcg tgccgcagtt caagcatagt
    14581 aaatgatgat ggaaatgttt ggcgccttct tttctcaaat cataacgggc taatccatca
    14641 ttaaaactta ctttatcgac aattttcaat tctgtcaaga tttcaagggt tcgataaaca
    14701 gtagcaagtc caatttccgg actctttttt ttcactaaaa agaagatttc ttctgcagat
    14761 aaatgctctt tctcattttc caaaagcaca agcagagtag cttctctttg cggtgtcagc
    14821 ttaaaccccg cttcatgtag ctgctgtttt gtttttttca tcgccgaaat agcattcatg
    14881 accatttact caaccccaac aatttataat aattacaaat aaaaaaagat ataacactat
    14941 gttaattaaa attattataa gctagttaag aaaaacatgc aagggattct caattagatt
    15001 ttttgactag ttttgtcaca ccgttttctt gtgtaatcaa tccttgtttc aaaagattac
    15061 ctaatgcgcg cttaaatgct cctttgctga taccaaaagc tttcatgatt tcttctggat
    15121 ccgatttatc tgtaaagttg atttgctgat ctgcagaacg ttctaaaatg gttaaaatca
    15181 tttgtgcatc atctgatata gcttcatatg ctcttggctt caatgaaaga ttcagtacac
    15241 cgtctggtcg gacaccaatc actcgacctt ttatctgctc gcctagacga ggttcattat
    15301 atcgttcaga aggatggata aacccaagat agaaatcttc cgttaaaacg tacgtacctg
    15361 ttaatttcaa gcggtatgct gttcctatca catctttgtt tttcatttct tctgttccat
    15421 atttgcttaa agatttgaat attttttcat ctgctaaact gccccaaata cggtcttttg
    15481 catcaactct aagggaaatc atcaagcgat cccctttttt aggccatagc tctgtcattg
    15541 tcggcaattc atcaagagat acgacaaagt ctttatctgg aagaccgata tcgacaaacg
    15601 cacctaaatc ttttcgtgta gcagtaacga ttccaaaagc gtaatgaccg atacggcttt
    15661 taggaatttg tgtagtgatc cgattttcat gtttttgatt ttggtaaccg aatccttcaa
    15721 ctgcttcacc gattttgtgt tcaccttctg atttgcttaa gccaaaggtt tgtccgtttt
    15781 tttggacaaa gtagtttttt tcattttcat caataataat acctgtaaat atagttgcta
    15841 ataaatcgtt catttttttc tccctctata gtgaaaggtt ttcttaatac atttctttgc
    15901 tatcataaca taatgttatc tacttgaata gctgagcctt ggtttggaaa ggtgaaaaaa
    15961 ggagtatgac aacagttgtg tcatactccc ctttttcgaa taaacggtgt taaacggcag
    16021 gctcacagcc tctttcattt gcctaaaatt aattgctccc atttcctccg aaccatccag
    16081 ttttacagtt gatggttacg atgtaaaatg gcaaagcact ttctccaatg cctttcatca
    16141 atgcagattt tgttgaagtg gcatatagat tataatagcc ttccccattt tcgattctgg
    16201 ctttttctaa tctgtaacct ccttcaacga tatatccacg ttggatacat gtagcaatca
    16261 ctttgctttg gacacctggt gcccacgtcc atgctggatt agatgcatcg gcaatatcac
    16321 ttgctctggc agatttgatt ggctgattcg ctgagtttga agaggaaggg gtcgaggatg
    16381 ccgcttcttg ctgtgctgct tgtgcgaccg cagcttgttg agcggcttga gctgcagctg
    16441 ctgcttgagc ggcttgtttt gcttgttctt cagctgcttt tttcgcttct tctgcttttt
    16501 tcttttcctc ttcttctttt ttctctttat cagtgagcat cttatctact tgtttcaatt
    16561 gttcattcaa ctgagcagat agatctgtat ttttcacttt ttctactgct tcttttgccg
    16621 tcttgtattg gtcgcgtgtt gctgaatcca ctacttttcc gtctttaaag actatagctg
    16681 ttttttcttt agctgtttgc aattcagcat attgattttt agcttctgtt tgtgcttgtt
    16741 gaatcaattt tccaaaatct gtgttatccg tttcgattgc ttggatctct ttatctgctt
    16801 tcaacttgat gtcatctgct agatgatcat cgacgatggc aggtgctatg aaccactcat
    16861 tcactttttg gatatctcgg atcttagatt ggatatcttg gtaagttgtt tctaactctg
    16921 aatatccttt ttcattctta agactggata aatcattttt tagttttgat aaatcttgat
    16981 gaatcatatc tgcacgaata aattgatggt gatcatctgt ataaaaggca gcaagtttct
    17041 catgaagttc ttccatttgt tgctgtcttt ttgcttctgc tgctacttcg gcttctactt
    17101 tttgatgatg gtcataataa gcccagcttc ctccaccgag tagaagtact gctgctaaaa
    17161 cgagataagg tacttttttt gattttttct tttgcgcttt atctggatct gttttttcta
    17221 agtttgattc ttgatgtgtt ttttcagtat ctggcacgat tgttattgat ttcgcttctg
    17281 cttcaaacag cttaggctgg ttctcttctt tttctatttt tgcttcaaga tcagtaccct
    17341 ctttgcttac agattgattt tcagcatcgg attcactatt tatttcatcg ggctctgttg
    17401 actttttgtc tgtgttttcg agtatttctt ttttctctgc ttccggcagt ttattcgttt
    17461 tttccttttc taattgtttt tcagtaaata cttcagtttt ttctgtgata tccgttttta
    17521 cgtcttttat ttttactgtt tctttacctg tggcgttttc tctatcagag tctttttcag
    17581 tttcttgttc cttttctggc tctactttgt tttcctgtgc aattttttca ctgatcattt
    17641 tgagcagctc tttcgcgtgc tcttcttttg tatcttcacg atgtgctttg atataagcgg
    17701 atagtatcga attttcttcc tcattatcga cgatttcttt cagttcagct actcttcttt
    17761 tttgttttga ttcaagcgtt tcttcttgat tctttcctga ttcttccgaa gtctcatgaa
    17821 cagttgcttt cttctgttca tttttctcta tattagttac ttgatcgtga gcaggtggtt
    17881 cttcgtcttc tggttctgct ttttcgaata attccattac agattctaga ggtaaatctt
    17941 tcaattccga ccattcgatt ggatcgttta attctgtatc aggaaagact gcatcttcag
    18001 ttgatttatc ctgcatagct gcatctgtat ttgtttcttc aattgattct tttatttgct
    18061 gatcttctgc agttatttga tgaccgcagt ctggacagat agtattctgc tccatttttt
    18121 tcgatccgca atttggacat tttttcatca cttagcgatc ggctccttta ctaggtttac
    18181 gctaccattt aatctatcat attctatacg tcacgccaat agcttatttt taggatttac
    18241 ttaaaaataa tgaatgaatt tccttcctat agaaaaaaaa gcttgagact gcgtagtctc
    18301 aagctttttt tcttttaaaa taaaccaaac agttttactg atttctcttc tggtcaatag
    18361 tccacgctta ttctaagaaa tcttttaatt gttttgaacg agaaggatgg cgcaattttc
    18421 ttaatgcttt agcctcgatc tgacgaatac gttcccgagt aacaccaaat actttcccta
    18481 cttcttccaa tgttctcgtc cggccatcat ctaaaccaaa acgtaagcga aggacattct
    18541 cttcccgatc agtcaatgta tctagtacat cttccaattg ttcttttagt aactcataag
    18601 ctgcgtgttc tgctggactt gttgcttcct ggtcttctat gaaatcacct aagtgagagt
    18661 catcttcttc accgattggc gtttcaaggg aaacaggttc ttgcgcgatt ttcaagattt
    18721 ctcggacttt ttctgttgga agatccattt ccgcaccgat ttcttctggc gtaggttctc
    18781 ttccaaggtc ttgcagcaat tgacgttgga tacgtatcaa tttattaatt gtttcaacca
    18841 tatgaactgg aatacggatc gtacgtgctt ggtcagcgat tgctcgagtg atggcttgac
    18901 gaatccacca tgtagcatag gttgaaaact taaatccttt gcggtagtca aatttttcaa
    18961 ctgccttcat cagtcccatg tttccttctt ggatcaagtc aaggaactgc atgcctcgtc
    19021 ctacataacg tttagcgata cttactacta aacgaaggtt tgcttctgcc agacgctgtt
    19081 tcgcttcttg gtcgccttct tcgattttca atgctaacgc tacttcttct tccgcagtta
    19141 aaagggagac acggccaatt tctttcagat acatacgaac cgggtcattg atttttacac
    19201 cggttggcgc agaaagatct tctgtttttg cttgttcagc ttttttctca gcactcttca
    19261 agctgtgaat tgatggatca ccattttcat ccaccacact gatacctgcg tcttcaatct
    19321 tttggatcaa tttgtccatt tcatctgcat tcaaagtgaa tggtgtagca agctgattgg
    19381 ataaatcatc atatacaact tgtcgtttgg gtttgttttc tttgatgaaa gctgctactg
    19441 ctgcttcata cgttttttta tctgttccat ttgccataaa agaaggcccc ccttgagtag
    19501 ctatttggct tgttttaact gtttcgttaa attgatgatt tcaatcgtca attcaagctc
    19561 aagctgtttg tttccagttc gtctggcttc ttgctgcatt tgttttttta atgttatttc
    19621 atcagctaag ccagatttgc tgatcacatc tagcaagtct tggatttctc gtggcgaact
    19681 ttcttctgcc accggtaaat attcgatttc caccaccaac cgccttaaat tttcatcttg
    19741 caaaaagtct aaaaactctg caaggttgaa ttcataatgt tgtgagacat atgcatcgaa
    19801 taaaaaataa acttctgcat atgcatcatg aataaactga aattctcgtt cttttaaaat
    19861 attacggaca gaaacttctt taaatgcacg atataataga atctgctctg ccttttctac
    19921 ttgcgtcaaa ggtctctttt tccgtgcagt ttcttgtctc gtaacctgag tgtgttgact
    19981 ttcctgaggt tcttgacgat tttgctgatt tgtccttcga agatcgcgca gttgtttttg
    20041 tatcgtctct ctgccgatct gaaactctgt tgacagttga gatagataca tatcttgttc
    20101 gattggtgaa acaactcggc taagttcaac tagtaattct tcgaggtatt cgatcttgtc
    20161 tttttcatta tctaaatttt tcccttgttt taaataacgt gatttaaaag taaagactgt
    20221 ttcgcgccca tgagccacta attcttggaa agatgcttct ccatacttac ggagatattc
    20281 atcaggatca agcttttctg gaacgactac gacagaaagt gctaatcgac tgttttgccg
    20341 aagcaattca atccctcgat tggttgcttc aaaacctgcg ttgtctccgt catagcaaaa
    20401 gaccaattct ttcgtcagct tttccatttg ttggatctgt tgagctgtta gacttgtccc
    20461 catagaagct aatccagttt tgataccagc ctgccatgca gcaatcacat ccataaaccc
    20521 ttcgaacaac aaaatttctc cgtttttccg gatatcgctt cttgctttat caagattaaa
    20581 taaaacttgt cttttattga acagttctgt ttctggacta ttcaagtatt tgggctgatc
    20641 tttttcctgc cctttttcag gtgcaagcca gcgtcctgaa aaaccgatcg tctggccttg
    20701 agaattacga agtggaaaca tcactctttg ataaaaacga tcagacatac ggccatcatc
    20761 atgcgtgaca aacaatccag agcgttctaa caattcattt gaaattgatt cattcttgaa
    20821 tacttgctga agaaaagaac gctcattagg tgcaaaacct atgttgaatt cttcaattaa
    20881 ttctaaagac aacccacgac tttgcaaata ttctaaagct ggctgtccag cttttgtatg
    20941 caatagcatg tgatgataga tttccgctgc tttttcatga gcagcaatca gtttccccgt
    21001 agcagattca ggattttgga cagctatagt acgctgacgc cattgatctt ctaacggtat
    21061 ctgttcgaag tctgccactt ttaccactgc ttctggaaaa cttagacctt ctagttcttg
    21121 aagaaaagta aacacgtttc cgcctttacc acaaccaaag caatgaaaaa tctgcttgtc
    21181 ttctgcaaca gaaaaagacg gtgtacgttc ttcatgaaaa ggacacagcc ccaagtaatt
    21241 tttaccagac tttttcaact ggacatattg tccaatcacc tcaacgatat tcgtttgact
    21301 gcgtactgtt tcgataacct cttgaggaat ccgttgagcc attttttcac cccctgctct
    21361 attgatatct tttgggttta acagaagata tttcaggaga ataaccggta aaaaatcgca
    21421 ctaactgaaa tcagtgcgat tttaaaggac acaattatac atgtttgatt gtatcatatt
    21481 tcattgcttt ttcaagtata acacatttct atctcattaa gcatcaattt gctactcttt
    21541 tcagtaactg attttgaata tcagccttcc agtacaatac tcgaagaaca atatttacga
    21601 gcatgaatac aaagaagtat cctacgctct ctcccaaccc taagtttact tccaaaattc
    21661 catggaacag cattgcagct acatatatcc ctacgatcgt cgttactgct aggaagatgc
    21721 gcaatgggtt gaagggataa caagctttta caactgccat gcaactgatc ccaatcagca
    21781 agtaatacat caaagtagta gtttcagcat ttccccatgc gaatgttttt ccaagtagat
    21841 acaccgcaat gatattcact actacaagca aggcatttgg taaagcatta ataagtgctg
    21901 taggcaaata acggtaagtg atttttcttt tatcttgttc aaaagataaa aagaaggaag
    21961 gatacccttc aatcgctaaa tcaatcagcg tgatttggat agggataaac ggaaatccaa
    22021 ttgctgtaac ggcacatata atcgataaaa taaatgaata aatcgttttg ataaaaaaga
    22081 tgcctgagac tttcgtcaca ttgttgacca ctcggcgtcc ttcaaacagg acttctggca
    22141 atgtagtaaa atcagagtcc aacaatacca gattcgcaat ctgacgtgtt gctccatcac
    22201 cttctgccat agcaatgctg caatctgctt ctcttaacgc caaaacatca ttcacaccgt
    22261 cgcctgtcat tgcaaccgtt ctgccgcttt ctttcaattc atttaccagc aatttctttt
    22321 gctgcggact cactcgtcca aaaaccgtat agcgatgagc agcttctcgc acttccattt
    22381 cttcagtaat ttgagaaaga tcgacatacg catcataatt cggtaatcct gcacgtctag
    22441 cgatattgga gacagtaaaa ggattgtctc cagaaatcac tttcagatcc acaccttcct
    22501 catgaagata agctagtgtt tcttgagcgt tttttcggat tggatcatcg atttcaaaca
    22561 gagcaaccgg ctcaacaaat gctggttttt cttcgttcaa ttgctgattt ggagcgatcc
    22621 ccaacataag tacgcgatag ccattcgctt gggctgtttt tagactgtcc ggaagccgat
    22681 cttcttccac caaacgttca ggcgctccta cgtacacgac tcccagttct ggaaattcca
    22741 tcgcgcccca tttgcgatca gaagaaaatg cgactttgtt tgtcacatca taccgattag
    22801 aaagctcaaa ataatctctc atagcccgca tagtgatgtt gttatcactg ctttctgcca
    22861 gatacgtccc cataatttct gggatggctt tctcgtacag cggatgtagt gcttctaccc
    22921 gctggatact cattttccct tcagtaatgg tccctgtttt atccaagcat aatgtatcga
    22981 catgggccag cgtttcaatt gaatacatgt cttgaacaag gattcgtttt tttgccagtt
    23041 tggttacccc tgtcgttaat gcaatactta ttagcaatac caaacccttt ggaagcatgc
    23101 ctaacaaagc tgctgcagat gcaataacag aaattttgat agaagcatcg cggatgaaca
    23161 gtgcttccaa aaatagtatg atccccaaag gaataatcac gaaactagtg aatttggata
    23221 ctttgcgaat cgaatctaca agttcagaac gaatagcttt gtgagtcttt gcctcgctag
    23281 tcaagcggac agcataatta tctgcaccta ctcggataac ttcagccatg acagaaccgc
    23341 ttgtcaaaaa gcttccagaa agcaattcag ctccagtttc tttgacaatc aaatcagatt
    23401 cgcctgtcaa aagagcttca tttgcttctg cccgtccaga tataacctgt aaatcggctg
    23461 gtatctgttc tcctgctccg atttttacca aatctcctaa taccagctct ttagtagaaa
    23521 tcgattgttc tttaccgtcc cggatcacta gtacttctga ttgggaaaca atcgataatt
    23581 tccgtacaag atttcttgca tgtatttctt gataaattcc aataacgata ttgacgacaa
    23641 taattgctaa atacgccatg ttggaatacg cacctacaaa taacagacaa atcccaatac
    23701 caaagttcag aaaattgaat aacgtcaata tattatcgct gaatatttgt tttgtacttt
    23761 ttgatgcatc ttcctggtag tcattttgct ggccagaagc cattttctga actacctctt
    23821 gctgtgtaag tccattcatt ttctttttct ccattttccg ccaactttca aaaataatca
    23881 cgtgtttaag tctagcgtgt tccaagatta aaaacaactt gtttttataa aatttataga
    23941 aaaacacacg aatgatttta caaaaaagat gccaaccttt ttgtttgaat cacccgtgtg
    24001 ctgtttttat tatcaatcta tctttactta tattgttcct ccaaacggct catccaccaa
    24061 cctagtgctg taccggctat aaaaagaaca ggacaagcta gtaccccagc aactcccaat
    24121 cccgcgtaat cggtaggaat gatcataatc gttgaagcaa aaactacacc taaaatgaaa
    24181 tggaatagct tgctatatgc ctttgagaaa atgtaatcca tgatttttga caatccaaga
    24241 acacaaagaa tcccaccaat tccgatcggc acaatcactt caagtcgtac ttctttaaat
    24301 ccgtcagcca tcgctttata caatcccata tacaccaaaa aattagaagg gctcaatcct
    24361 gggacgatca tacccaaacc aatcaatccg ccggcaatca gccatgttcc aaaattctga
    24421 tcgacttgcc cgtcaaataa ttgagcgccg tataataaaa acaaataagc cagaacaaaa
    24481 gtaacaacca tgatgatcac atcagaagta tctcttcctt ttttccctgc ttctttccat
    24541 aacgcaggaa cagttccagc gatacatccg acaaaaaacc ataatatcgt tgtttcatac
    24601 gcacctaata aaaaactgac taaaaaagaa agtagaaaga tgcctgtcac tccgcctaga
    24661 ccaacaggca gaaaaaacaa aacattttct ttgaaatttt ttgtaaggtg ggctaaaaaa
    24721 gaaatcagcc gctcatacat gccaaagaca gctgctaatg caccaccgct gactcctggc
    24781 aagataaatc ctgaaccgat aaacattccc ttcacaaacc gtaacatcca gtctttggtt
    24841 gtgtacccca cttcttgatt ttccattttt tgcctttttt ccactatttg cgcactcctt
    24901 ctttcattta attttattca tgattgaaca tttcctgcta ttttacttaa agactttttc
    24961 ataggtctct attatttttt cattcatttt gacaatatca ggataaaaca tgttacctcc
    25021 attatgatag agatagccgc cattgtaaag caaggactgg atccgccaat atcggtattg
    25081 tttcccttcc tcatttccta aaagtgggct cagcacatct ttagaatacg cttctgctaa
    25141 ttctactgtg ttttctccgc catgattttt tacataggca atataatctt tcccaaaatt
    25201 atatgcttgg acagctgtcc ataaatcgca tccttgtttc tttgcttcta ctatcatttc
    25261 cgccaaatat ttgacacctt gatcaatact gtcagctgga ttatcaatga tctcagcttc
    25321 tccatgcaga ctttccgagc tctgcatgag atctgtccct tttccttttg attcggtcaa
    25381 tattatcgcc ttgatcaaat tctcgtagtt ttccatatga taattttttg cagctgttgt
    25441 tatttctatt tgatagcttt ccactcgttt taccgaacga taagttcttc ctcctaaaaa
    25501 caacaccgca ccaataaccg tcagaatgat catgtaaaaa agaatacgga taatagataa
    25561 tcgttttcgc ttcatttcat cacctagtta ttttgacatc tttttttatc ttactatttc
    25621 tttatcttct tggctatttc ttccttgatt tctgtgtacg ccttaagaaa aaatgaattt
    25681 tcctgtattt tttgacatct tacgaaaatg atcgttgttt ttcagtaaaa acaattgccc
    25741 aatgactgat ataatcgtta tactttacac agtaggtgat tatgtgaaaa aagaaaagaa
    25801 atatgcaaaa atgccagatg ggagcgaaat ttactatgaa aagagcggtc aaggcttccc
    25861 tctcttttta ctgcatggca atgacggcag cggccgcttt ttttcagaac aggtccctgt
    25921 actcgaacgc tactataccg tgtatctagt agatagccga ggacatggtc gttcgacaaa
    25981 cgaagcatct atgttgaatt ttcagttaat ggccgaggat ctgaatacga tcatgttgct
    26041 ggaaaaaatc gatcaggcag attttcttgg atttagtgat ggagcaaatc tagcactggt
    26101 atttgccagc agcttcccta aaaaagttca tcgattgatt ttaaattctg gaaacacgct
    26161 tgttaaaggc gttcgttttt ctgccagagt catcagtaat attcattatg catgggtatg
    26221 gttactctct ttatttcgcc caagtcttcg caaaaatctt ttagttatca aacttttgct
    26281 gcatgacatc gggcttacag aaaatgattt aaaaaaaatc aactccccca ctttgatcat
    26341 cgtgggaaaa aaagatgtaa tcaagctaaa acattctttg tatatagcta aaactatacc
    26401 aaaagcttcc tttgtgcttg tgaaagaaca aggacatgaa ttagctagaa aagatcctga
    26461 acgcttcaat cgagaagttc ttcaatttct ttctgaaaca taactattag gaatagaagg
    26521 tgatttattt gttaaaaaat accatacaat ggctgaaaaa ccacctagga ctgttcaaaa
    26581 caatcttttt gatttctgtc attgtcatta ttgtcggtga attgatgtcc atcggtaaaa
    26641 ctctatctat ccagcaatta ggagaaacgt ttgccactat acctgtatgg aaaagcggac
    26701 taatgctatt gattggtctt atctctgtgt tacctatggt tggttatgat attattttga
    26761 atcggatgct tgaacaaaag cagaatcctc gttacctgtt tgagacaagc tggctgatca
    26821 atacgatcaa caatattgcc ggtttcggcg ggtttgttag tgtggggctt cgctctgaac
    26881 tatatggaca aaagaaagag agcagaaacg tgatccaagc attatcgaaa gttttcttgt
    26941 ttttgatggc tggactttct atttacagtc tgctttcttt tttacttgta gtcttcacgc
    27001 cagttgctcc ttttttgaag caatattgga tatggttgat cggcggcggt ctatattttc
    27061 ctgtcgtctt tttatttact actttcaaaa agaaaggatt ccttggtggt acctcactta
    27121 aaactcaagg acagctattg ctagtttcat tgttggaatg gaccggtgtc ctgtttagtt
    27181 tcctttctgt tggttattta atggaaatcc ggattgatct gtggcaaaca attccattat
    27241 tcattgctgc ttcagtgata ggaatcgtgt ctatgattcc aggagaaatt gggagtttcg
    27301 atgtaatgat gattatcggg ttgtccgcta ttggtgtgcc aagagaaata gtcgttgtgt
    27361 ggatcttgct ctatcgcctg ttctactaca tcattccgtt tttgattgga atcgtctttt
    27421 tcttcaaaaa tatcggttcc acttttgatc agcgttattc tggtatccct aaacaattag
    27481 caacagaaat tgcgcataaa atcgtggttg tcttattata cttttctgga atcatgctag
    27541 tactttctgc cactatcccg caagctttta cagagtttcg ctggcttcat agtttgaacc
    27601 cattgaaatt tcattttatt atccaatttc ctagtatact attgggattt cttttaatca
    27661 ttatgggcag agggatcgca gcacgtgtga aacgagcata tttgcctaca atcttcttga
    27721 ttgttctagc tttactttat gttttattaa gtgattttag tttcacaccg gtggttttct
    27781 tgtctattct gcttctgatc attcttgctt ctaaaaacga attatttcgg gaacaattga
    27841 tctattcatg ggagtggcgt acgatcgatg gaatcattat tggtgcactg tccttgcttt
    27901 acatcgttat tggggtatac aatctgccag atttccctca tcggcgtcac cattttattt
    27961 cctttttctt atttccttct gaaaagattt ggttttcggg attgttggcc atcatcgctg
    28021 ttagtttcat gatcgttcta tttgttcatt tccttcaagg agaaaagaaa cagattggcg
    28081 aagcctttaa tgaagaaaag gccctgaaga tccttacaac ttatggagga aactccgata
    28141 gtcaattgat cttcttaaaa gataagcgga tgtttgccta tgaaaaagac ggtgaaccaa
    28201 ctgttcttct ccaattcgct tgcttcaata ataaatgtat tgtaatgggt gatccttctg
    28261 gtaagaaaga agattttcca gaagcaatcg aagcttttat cgaagaaaca gatcgactat
    28321 gctatttacc agttttctac gaaacaagcg aagagattgt tatgattttg catgaattcg
    28381 gctacgattt cattaagatg ggggaagaag cttatgtaga cctgaatagt tttacaactt
    28441 cagggaaaaa aatgaagggc actcgtgctg tcttgaatcg gatcgaacga gaaggattta
    28501 catttgatgt cttacaacca ccattttcta ctgagcaaat gagtactttc aaaaatatct
    28561 ctgataattg gttgggttca cgaaaggaaa aagggttttc tttaggcttt ttctctgaag
    28621 attatctaca aagagcgcct attgcagttg tcaaaaatac ccaagaagaa attgtcgctt
    28681 ttgcaacgat catgcctact tacacgaaca ataaagtagg aacgattgat ctgatgcgat
    28741 atgattctaa aaaagcacct tctggcagta tggatttctt gttcttgcat ttgtttgcct
    28801 atatgaaaga agaacatatc caatggttca atttagggat ggcccctctt tcaaatgtcg
    28861 gaacttccag aaagagtttt ttacaggaaa ggattgcata cttggtttac gagtttggtt
    28921 ctcattttta ttctttccat ggattaagag aatataaaag caagtacgct acaaactggg
    28981 tctcacgtta tactctatat tctagagata gttggattgc atatgttatg attgctttgc
    29041 tgattattga taatgcgcca gttgagcaga cacaaggtag aataagtggt ttgaaaaaat
    29101 ggatacgaag aaagtattaa aaactgcata ccgatagatg aacactctca tgatgaataa
    29161 aagagactga agtaaaacga aagttcatgc ttctacaatc tcttcttata caattgaaga
    29221 acaaagagcg gtccaaacag aaatcctgtt tggaccgctc ttttatttta aacatttttc
    29281 attaccaaaa tattcgagtc gcgtttctta tgcttcgatc tctgtaacga tacctgaacc
    29341 tactgtccgt ccgccttcac gaattgagaa tgtcgttccg ttttcaacag cgattggatg
    29401 gatcaagtca acatcaatcg tcacgttgtc accaggcatg accatttctg tatcttctgg
    29461 caatgtgatt gttcctgtaa catcagttgt gcggaagtag aactgtggac gatagttgtt
    29521 gaagaatgga gtatgacgtc caccttcttc ttttgtcaac acgtacactt ctgctttgaa
    29581 ttttgtatgt ggagtaatgg aacctggttt agcaagtact tgtccacgtt cgatatcgtc
    29641 acgttggatc ccccgtaaca acacgcctac gttatcccca gcttccccgt aatctaacgt
    29701 tttacggaac atttctacac ctgttacaac tgctttttgt gtttcaggtt tgatccctac
    29761 gatctctacc tcatcaccga cacgaacagc accacgatca atacgacctg aggcaacagt
    29821 tcctcgacct gtaattgaga agacgtcttc cactggcaat agtaatggtt tatctgtatc
    29881 acgttctggt gttgggatat attcatctac tgtatccatc aattccataa tagcagcttc
    29941 agcatctgga tcgccttgca atgcttttaa tgcggaacct ttgatcacag gagtgtcatc
    30001 gcctgggaaa ccatattcgc tcaataactc gcgaacttcc atttctacca aatcgatcaa
    30061 ttcttcatca tcgactaaat caactttgtt caagaaaaca atcaggtatt ttacaccaac
    30121 ttggcgtgac aataaaatgt gttcacgtgt ttgaggcatc ggaccgtcag ttgcagaaac
    30181 aactaagatt gcaccatcca tttgtgctgc ccctgtaatc atgtttttca catagtccgc
    30241 gtgtcctggt gcatcaatat gcgcgtagtg gcgtttttct gtttcatatt ctacgtgtgc
    30301 ggtattgatc gtaatcccac gttcacgttc ttctggagct gcatcaatac tagcatagtc
    30361 ttgaggattt gctaggcctt ttttgcctaa tactgtagtg atcgcagcag ttaaagtagt
    30421 tttcccatgg tcaacgtgtc caatcgtacc aatgttaacg tgtggtttac ttctgtcata
    30481 atgttctttt gccataattt acaaacctcc taaataatat agatcactga aaaatttcag
    30541 ctctcacctt tttattatag tctttttttg actaaatcaa aaagaatacg tccgacttac
    30601 tttttgtaag attcgaacta agcttattat acgtcaatat tggtcaaagt caactaagag
    30661 cactcgtctg tcttatacgc gaaaaaagta ccttttataa ataaaaaaat gtgttttttg
    30721 agaaaaactt caagatagtc cttaactttc ttttgaagca ataagaacaa aatgaggaaa
    30781 agtttaaaat tcagctgttg ccaacaactt tcaatagacg aaatcttcta tttcgttaat
    30841 aataaggaaa gaggtgaagt acatgaaaaa attaattggc gtacttgtcg ttttaggcat
    30901 atttctcggc ggaagttttg cagcttatca ttatttttac ggcggcgaag cttattatac
    30961 aaaaatcacg acgaatggcg aaatgtcagc tacccaagca gataacggag agaaattcac
    31021 gacttatacc tacaagcaag cggcttataa taaagatggt gaaaaaaaac aggtgacgct
    31081 tcgcgaagaa agagaacatc cattaaagat gaatgcgtat cttaaactca aagtcaatcc
    31141 tcgtaaaggt gtattaagct gggaagaaat aaaggcttct gaagtgccta aaaatgcggc
    31201 agaaaaaatc gataattagt aaatggtctt aaaattttat tagataaaa
//
LOCUS       DACEUF010000027        30947 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.028, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000027 DACEUF010000000
VERSION     DACEUF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.028"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            82..1395
                     /locus_tag="IXG20_001184"
     CDS             82..1395
                     /locus_tag="IXG20_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1609393.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            1392..2111
                     /locus_tag="IXG20_001185"
     CDS             1392..2111
                     /locus_tag="IXG20_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ1609394.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            2239..2790
                     /gene="raiA"
                     /locus_tag="IXG20_001186"
     CDS             2239..2790
                     /gene="raiA"
                     /locus_tag="IXG20_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ1609395.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            3012..5546
                     /gene="secA"
                     /locus_tag="IXG20_001187"
     CDS             3012..5546
                     /gene="secA"
                     /locus_tag="IXG20_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ1609396.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            5672..6773
                     /gene="prfB"
                     /locus_tag="IXG20_001188"
     CDS             join(5672..5734,5736..6773)
                     /gene="prfB"
                     /locus_tag="IXG20_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ1609397.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            6878..7564
                     /gene="ftsE"
                     /locus_tag="IXG20_001189"
     CDS             6878..7564
                     /gene="ftsE"
                     /locus_tag="IXG20_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ1609398.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            7548..8441
                     /locus_tag="IXG20_001190"
     CDS             7548..8441
                     /locus_tag="IXG20_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1609399.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            8593..10041
                     /gene="cls"
                     /locus_tag="IXG20_001191"
     CDS             8593..10041
                     /gene="cls"
                     /locus_tag="IXG20_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1609400.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGGKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            10378..11235
                     /locus_tag="IXG20_001192"
     CDS             10378..11235
                     /locus_tag="IXG20_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ1609401.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            11350..12270
                     /gene="pstC"
                     /locus_tag="IXG20_001193"
     CDS             11350..12270
                     /gene="pstC"
                     /locus_tag="IXG20_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ1609402.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            12270..13154
                     /gene="pstA"
                     /locus_tag="IXG20_001194"
     CDS             12270..13154
                     /gene="pstA"
                     /locus_tag="IXG20_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ1609403.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            13169..13975
                     /gene="pstB"
                     /locus_tag="IXG20_001195"
     CDS             13169..13975
                     /gene="pstB"
                     /locus_tag="IXG20_001195"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609404.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            13991..14749
                     /locus_tag="IXG20_001196"
     CDS             13991..14749
                     /locus_tag="IXG20_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609405.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            14762..15439
                     /gene="phoU"
                     /locus_tag="IXG20_001197"
     CDS             14762..15439
                     /gene="phoU"
                     /locus_tag="IXG20_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ1609406.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            15677..16708
                     /locus_tag="IXG20_001198"
     CDS             15677..16708
                     /locus_tag="IXG20_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1609407.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            16705..17472
                     /locus_tag="IXG20_001199"
     CDS             16705..17472
                     /locus_tag="IXG20_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1609408.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            17572..19140
                     /gene="liaX"
                     /locus_tag="IXG20_001200"
     CDS             17572..19140
                     /gene="liaX"
                     /locus_tag="IXG20_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ1609409.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            19440..19757
                     /locus_tag="IXG20_001201"
     CDS             19440..19757
                     /locus_tag="IXG20_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ1609410.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            19757..20107
                     /locus_tag="IXG20_001202"
     CDS             19757..20107
                     /locus_tag="IXG20_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ1609411.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            20274..21212
                     /locus_tag="IXG20_001203"
     CDS             20274..21212
                     /locus_tag="IXG20_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ1609412.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            21227..22057
                     /locus_tag="IXG20_001204"
     CDS             21227..22057
                     /locus_tag="IXG20_001204"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1609413.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            22101..23126
                     /locus_tag="IXG20_001205"
     CDS             22101..23126
                     /locus_tag="IXG20_001205"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1609414.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            23141..24079
                     /gene="galU"
                     /locus_tag="IXG20_001206"
     CDS             23141..24079
                     /gene="galU"
                     /locus_tag="IXG20_001206"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1609415.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            24204..24644
                     /locus_tag="IXG20_001207"
     CDS             24204..24644
                     /locus_tag="IXG20_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ1609416.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            24647..25249
                     /locus_tag="IXG20_001208"
     CDS             24647..25249
                     /locus_tag="IXG20_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1609417.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(25312..26415)
                     /locus_tag="IXG20_001209"
     CDS             complement(25312..26415)
                     /locus_tag="IXG20_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1609418.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            26625..27626
                     /gene="ccpA"
                     /locus_tag="IXG20_001210"
     CDS             26625..27626
                     /gene="ccpA"
                     /locus_tag="IXG20_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ1609419.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            27981..28376
                     /locus_tag="IXG20_001211"
     CDS             27981..28376
                     /locus_tag="IXG20_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609420.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            28376..28627
                     /locus_tag="IXG20_001212"
     CDS             28376..28627
                     /locus_tag="IXG20_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609421.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            28670..29065
                     /locus_tag="IXG20_001213"
     CDS             28670..29065
                     /locus_tag="IXG20_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609422.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            29062..30318
                     /locus_tag="IXG20_001214"
     CDS             29062..30318
                     /locus_tag="IXG20_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ1609423.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            30330..30653
                     /locus_tag="IXG20_001215"
     CDS             30330..30653
                     /locus_tag="IXG20_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609424.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            30646..30912
                     /locus_tag="IXG20_001216"
     CDS             30646..30912
                     /locus_tag="IXG20_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609425.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        10412 a         5327 c         6670 g         8538 t
ORIGIN      
        1 aatagaacag tataactatc gaacaagcga tccaaaaaat agatcgcttg ttttttcgta
       61 tattttttta gggcgaataa tatgaatcat ttttggggta gaaggcttct gacgagagat
      121 attaaagcaa tagactgtga ggaggtaaat ttaccaaatt atgagaagat cgcagcagtg
      181 gaacgtttgg gcaactggat tctttgtcat cgctgtggaa tgaggacgtc cgtcattgat
      241 ggacagcttg cagattacgg ttatttttgt acacattgtc tatatttagg gcgatgcgac
      301 actcaacaag atttatattt gtttgatcag cctgaagcaa aacctcgaga agtcgtcttt
      361 ttttggacag gacagcttac tgaaaaacaa acggaaatcg caaagagaat actttgccat
      421 tcagaaaaaa gacatcatct tatttgggct gtaacaggtg cagggaaaac agagatgctt
      481 tatcctattt tagtggaaac tttaaaagct ggaggaagag tagcgatttg tacaccgcga
      541 gtcgatgttt gtaatgaatt gtttttacgc tatcgttatg tttttccaga agaaaaaatc
      601 acgttgctcc atgggaatac agcaacggct tataccttta cttcttttgt tatctgtacg
      661 attcatcagc tataccgttt ttatcggtcc tttgatttaa tagtgatcga tgagatcgat
      721 gcgtttcctt ataacgggga tgcaggatta gctcatgctg tgcaaacagc gattaaacca
      781 gacggacgat tcatctactt aagtgccaca cctgataaaa agttactaaa ggaaatcaag
      841 cagacgtttg acatccacaa acttgctttg cgctttcatc ggcgtttact tcctgaaccc
      901 gtcttggttt tcatgaataa ttggcgacaa aaatgcaaga acaagaagaa gataaaatct
      961 ttggtgcgga tcattcatca attaattaaa gaaaaccata tccttttatt ttgtccaagt
     1021 atcacgatga tgcatcgttt gaaagccaca cttcaagagg ctcttccgag atatgagatc
     1081 acagatgttt cttcaagaga caatcagcgg gcggaaaaag tccagaaaat gcgagaagca
     1141 aaatatgaca tactgttaac cacgatgatc cttgaacgtg gagtcacatt tgaaagaata
     1201 tccgttatcg ttttaggagc ggatcatccg gtttttacta aatcttcttt ggtacaaatc
     1261 gcaggaagag ccgatcgtaa agggccttat acgaacagtc aagtatactt tttctatgaa
     1321 gaaaaaactg cagctattcg aaaagcttgt caggaaatca aagaaatgaa tgaagaaggg
     1381 aagaagatgc tgtgaggtgc agttgctgca gtaaagatat cgtccaaaat ttaacattca
     1441 gcgagattat tggtgtttcg aaatacagat tacctcagac ttgttcagtt tgtcgcgcac
     1501 aatttcattt attggaaaca agaggctgtt ctggctgtca aaaaccaatc catttcaatc
     1561 tagagagtac gaaaaagaaa gtaaagtcta ttcccgttta ctgcagtgac tgtcaagaat
     1621 ggaagaagaa atatccgacc tatgattttc agcaccgtgc cttttttgtg tacgatgaaa
     1681 atatgaaggc ctggctcaat caatacaagt ttatgggaga tatccgacta gcaggcacct
     1741 ttgcatataa atggacgcag ataaaaaaag aatacccgac atacatctat tgtccaatac
     1801 ctttatctga aaaaaggcga acagaaagag gattcaatca agtaagcgag atgttgaaag
     1861 cagcaagaat tccttttcag atgctactca aaagagagaa gcatctgctg gctcaagcaa
     1921 aaaacacgcg aaaggaaaga ttggagatgc ctcaaccttt tgaacttgca gtcacctttt
     1981 ctaagataca aaataaaaaa atattgatta ttgatgatgt ctatacaaca ggacagactc
     2041 ttttccacgc agcagattgt ttgcaggtcg cacagccgaa aatcatccga accatttctt
     2101 tagcaagatg aattagatgt ggaaatttga tagcgattta atttgcaaat aacctagagt
     2161 tcgatataat agaattaaga agagggaaag agtggtcctt ctccttcttg attgtggtag
     2221 acgaaagggg taatttttat gtttagatat aatgtacgcg gcgaaaatat cgaagtaact
     2281 gaagcgattc gcgactacgt tgagaaaaaa gtaggcaaac tagaacgcta tttcagtgac
     2341 gcacccgatg caacggcgca tgtgaactta aaagtttata cagaaaaaac agcaaaagtg
     2401 gaagtaacga tcccattacc ttatcttgta ttacgtgccg aagaaacttc tccagattta
     2461 tacgcaagta tcgatttggt tgttgacaaa ttggaacgcc aaatccgcaa attcaaaaca
     2521 aaaattaatc gtaaatcccg tgaaacgggc atgaatactg cagatgctac tgctttattc
     2581 aatgaagagg acgaaaacga aaatgattct gaattagata tcgtacgtac gaaacgcctt
     2641 tcattgaaac caatggatag tgaagaagca gtcttacaaa tgaacatgct gggacataac
     2701 ttctttattt ttgaagatgc agaaacaaat ggtacaagta tcgtttaccg ccgtaaagat
     2761 ggaaaatacg gactgattga aacagactga tcagccttac ttaaataaca acgaaaactt
     2821 gcagcagcta ctgacagctg ccgcaagttt ttttgagttt cctcctcttt tagatgaaat
     2881 atgaataaat tatgaagttc ttactttaat acgcttaaga tatttcaatt atctataact
     2941 aatgataaaa tgaaaagtaa agtctacata gtctggataa tacacagaag gaaaaggaga
     3001 ttacacaacg catggccaat ttgattcgtt cacttatcga aaacgataag aaagaactaa
     3061 aacgtttaga tagcattgca aaaaaagttg aagcatacgc tgaccaaatg gcagcattaa
     3121 ctgatgaaca attacaggca aaaactgctg aattcaaggg cagataccaa aaaggagaaa
     3181 cattagatca attacttccg gaagcttttg cagtcgtccg agaagcagca aaacgtgtat
     3241 tggggttata tccacaccac gtacaattga tgggtggaat cgtgcttcac gatggaaata
     3301 tccctgaaat gcgtactggt gaaggtaaaa ccttgaccgc aacgatgcca gtatatctaa
     3361 atgctctgac tggcgaaggg gtacacgtag ttaccgtcaa tgaatattta gcaacccgtg
     3421 actcgacgga aatgggcgaa ctttataatt tcttaggatt aacagtgggg ttgaatatca
     3481 actcaaaatc ttcggatgaa aaacgcgagg cttataattg tgatatcact tatagtacga
     3541 ataatgaatt aggatttgac tacttgagag acaatatggt cgtttaccgc catcaaatgg
     3601 tacaacgtcc gctgaattat gccattgtcg atgaagttga ctcaatcttg attgatgaag
     3661 cgagaacgcc gctgatcatt tcagggcaag cagaaaaatc cacagcttta tatacaagag
     3721 cagacaactt cgtgaaacgt ttgaaagaag aagaagatta taaaatcgat gtacaatcaa
     3781 aaacaatcag tttaaccgaa gcagggatcg aaaaagcaga agaaacattc ggtttggata
     3841 acttatacga cattgaaaat actgcattga cccatcactt ggatcaagca ttgagagcca
     3901 actatatcat gcttttagat atcgattatg ttgtccaaga cggaaaagta ctgattgttg
     3961 accagttcac tggtcggatc atggatggac gccgttattc agatggactt caccaagcaa
     4021 tcgaagctaa agaaggcgta gagatcgaag acgaaacgaa aaccatggcg acgatcactt
     4081 tccagaacta tttccgtatg tacaagaaac ttgcgggaat gactggtaca gctaaaacag
     4141 aagaagaaga attcagagaa atttataaca tccaagtata ccagattccg acaaatcgtc
     4201 cggttatccg tgatgatcgt ccagatttgc tttacccaac attacaaagc aagttccacg
     4261 ctgtcgtgca agatatcaaa gatcgctacc gcaaaggtca acctgtgtta gtcggaactg
     4321 ttgcggttga aacatctgag ttattatcag atatgctgaa tcaagaacgt attcctcatg
     4381 aagtgctgaa tgcgaaaaat cactttaaag aagcagagat catcatgaat gctggtcaaa
     4441 aaggtgctgt gacgattgct accaacatgg ctggtcgggg aacagatatc aagttaggac
     4501 ttggggtaag agaacttggt ggtctagcag ttatcgggac agaacgacat gaatctcgtc
     4561 gtatcgataa ccagcttcga ggacgtgcag gacgtcaagg agaccctggt atgtcacagt
     4621 tctacttgtc attggaagat gatttgatga aacgattcgg ttcagaacga atcaaatctt
     4681 tcttagatcg gatgaagatc ggtgaggaag atgctgttat ccaaagcaag atgctgacga
     4741 aacaagtaga atctgcacaa aaacgtgtag aaggaaataa ctatgacaca cgtaaaaacg
     4801 tcttacaata cgatgatgtg atgcgtgaac aacgtgaagt aatctataaa cagcgacaag
     4861 aagtaatcat ggaagaaaaa agcttatcca agccactaat gaacatggtc aaacggacaa
     4921 tcagtcgtgt agtagatgcg catacacaat tagagaaaga aaattggaat ttggaaagta
     4981 tcatcgattt tgccggcagt actcttgtcc atgaagatag tattactctt gcagatatcg
     5041 aaggaaaaac agctcaggag atcaaagagt atctaaacga acgagcacaa gaagtattta
     5101 ataccaaagc ggctcaacta aatggtccag agcaactgtt ggaatttgaa aaagtcgtga
     5161 tcttgcgtgt tgtggactct aaatggacag accatattga tgcaatggat caattacgtc
     5221 agtcaatcgg cttacgtgct tatggacaga acaatccatt agtcgaatat caaacagaag
     5281 gctataagat gtttgaagat atggtaggcg ccatcgagta tgaagtcact cgaatcttta
     5341 tgaaagctga gatccgtcaa aacgtacagc gtgaacaggt ttctcaagga acagcttctc
     5401 actcagaaga cggagatgcc aaagaaaata gtaatactag cgcgaaaaaa cagccaatcc
     5461 atgtgaacaa ggtcggaaga aatgatcctt gtccttgtgg aagcgggaaa aaatataaga
     5521 actgtcatgg aaaaaatgca aactaatttc tgacagcagg attggtgaga caaattcttt
     5581 ttttaagaat tcgtctcacc ttctttctgt tatttgctga ttctgttaga ctagatataa
     5641 tgaaaaaagt atagtgaaag aaggaaaaaa catggaaata tccgaaattc gaaatctcct
     5701 gacagaaatg caggaaaagg tcacaagctt caggaggtct ctttgactta gaccagctgg
     5761 aagaagacat tgcggaagca gaagcaaaaa tggctgaacc tggtttttgg gatgatagcg
     5821 aagctgctca aaaagtgatc agtgaaaaca atgcaaataa agaaacttat gatcaattcc
     5881 attctcttgc tgaagaactg gaagaaatcg aattgatggt ggagatgttg caggaagaac
     5941 ctgatgcagc catgcaagaa gaagcagaaa cacgtatcaa tgcgctagat gaaaaaatga
     6001 agacatatga attatctatg ttgttagatg gtccttatga tcgaaacaac gccatcgttg
     6061 aattgcatcc aggggctggc ggaacggagt cccaagattg gggcagtatg cttttacgta
     6121 tgtatacacg ctgggcagag cagcatgggt atcaagtaga gacactagat tatcaagcag
     6181 gtgatgaagc aggaatcaag agtgtgaccc tactgatcaa aggacacaac gcatttggtt
     6241 atctgaaatc agaaaaaggt gttcatcgcc ttgtacgtat ctcgcctttt gactcagcga
     6301 aacgacgtca tacttctttt tgttcggtag atgtgatgcc agagcttgat gaaacgatcg
     6361 atatagaaat caatccagat gatttgaaaa tcgatacgta ccgagcaagt ggtgcaggtg
     6421 gtcagcacat caataaaaca gaatctgctg tacggatcac tcacttgcct acaggaacag
     6481 tagtagctag tcaggcacaa cgttcacaat tgaaaaaccg tgagcaagcg atggggatgt
     6541 taaaagctaa gctttatcaa ttagaagtgg aaaagaaaga acaagaagca gctgcacttc
     6601 gaggagaaca aaaagaaatc ggctggggtt cacagatccg ttcttatgtt ttccatccat
     6661 attctatggt aaaagaccat cggacaaacg tagagacagg aaatgtccaa gcagttatgg
     6721 acggtgacct tgatttattt attgacggct atttaaaaat gcatctacaa taagaaccca
     6781 ttgttaaatt gaaataaaat tgtaagattt tgcaagcatg ttgaaacatt gccatgctat
     6841 aatagctaaa gactgaaaaa gatatggaga gaacgccatg attgaaatga aggatgtaat
     6901 gaagaagtac tcaaatggta cgaccgctat ccgcaatctc tcaattgtca tcgaacaagg
     6961 agagtttgtc tatgtggttg gtccgtctgg agccggtaaa tctacattca tcaaattgat
     7021 gtatcgagaa gaaaaagctt caaaaggaac tttgaacgtc gcaggtcatg atctaatagc
     7081 aatcaaaaac cgcgaagttc cttatctacg cagagaaatc ggcgtggtgt tccaagacta
     7141 taaattatta ccaagaaaaa cagtatatga aaatgttgcg tacgctatgc aagttatcgg
     7201 cagaaaacct cgggatatca aaaaacgagt aatggaagtg ctggaccttg ttggtttgaa
     7261 acacaaagtt cgagtctttc ctagcgaatt atccggtggg gaacagcaac gtgtctctat
     7321 tgctcgggca atagtgaata cgcctaaagt acttatcgcg gatgaaccga caggaaatct
     7381 ggatccagaa aactcatggg aaatcatgaa attattagat cggatcaatg cccaaggaac
     7441 tactgtcgtg atggctacac ataacagtac gatcgtaaat acgattcggc accgagtaat
     7501 tgctatcgaa aacggccgca ttattcgaga tcaagcggaa ggggaatatg gatacgatga
     7561 ttagaacctt tttccgccat ttattagaaa gtatcaagag tttgaaacgt aacggctgga
     7621 tgacagttgc ttcagtcagt gcggtaacga tcacattgac tttagtcggt gtattcatgg
     7681 cagttatcat gaacgcaact aaattagctc aagatataga aggaaacgtt gatgtctctg
     7741 tattcgtaga tatcggtaca aaagacgaag agatgaaaaa gctagaaaaa gaattgaatg
     7801 acttagatca tgttaaaaaa gttgaatttt caagcaaaga acaggaattg aaaaaaatcc
     7861 aaaacgaaat gggtgattcc tggaaattgt ttgaaggtga caataacccg ttatatgatg
     7921 tgtatgtagt aagtgctact gatccttctt atacaaaaca agtagcagaa gaagcagcag
     7981 atttgaagaa tgtttctcgt tctgattacg gtggagcttc ctcagatcgg attttccaaa
     8041 tcgctggtgc agttcggaca tggggattag ttgcagcggc attgctgctg ttcgtagcga
     8101 tgtttctcat ttcgaacacg attcggatca cgattttgtc tagacagcgt gaaattcaaa
     8161 tcatgcgttt agtcggtgca aaaaatggat atattcgctg gccgtttttc cttgaaggtg
     8221 gctggatcgg tctgatcggt gcgattcttc cgattttgat catcacattt ggttatgcat
     8281 ggtttttcca attgatcaat ccatcattat tacgttccca ttattcattg attcatccac
     8341 aaaatatcgt ttggaaaatc aatcttttaa tggtcggcat tggtgtgatc attggttcac
     8401 ttggctcgat catttctatg agacgtttct tgaaattcta ataccacaat ataaataaaa
     8461 tgagaaagtt ggacagtttc gttccagctt tcttttttta ttggctagga gaatagaagg
     8521 aagcggcttt tttcccttta ctttaaaaaa caacttgggt attcttaagt tggtagaaga
     8581 agaggtgaag ccgtgaaaat tatcgttgac aattttttta cgatcctttt gattatgaat
     8641 attttgcttt cctttatcat cgtttttcga gaaagaaagg aaactgctca aacatgggca
     8701 tgggtgctcg ttttgatgtt cattccagtt gtaggattta ttttatatat ttttttagga
     8761 agagggattt ccaaagagaa aatctttgat ttgaagatcc aagggaaaat cggtaagaac
     8821 ctggaaattg aagaagacaa acaggcattg atgcgagggc tttatcctca tccgcctact
     8881 ggacaagtag atgtgaaaca attgatctat atgctcacgg tatttgaaag tacgctctat
     8941 acgactaaaa atgaaatcac tttatttaca gatggtcgtg aaaaattcga tgcgctgatt
     9001 gaagatatca agcaggcaga agaccatatc cacttccagt attatattta tcgcagtgat
     9061 gcattaggtg aagaagttcg agatgcattg acagaggcgg caagacgagg gggaaaagtc
     9121 agagtccttc ttgatgcatg gggttccaca caagtttctt ccagcttttt tcagaatttg
     9181 aaaaaagcag gcggagaagt cgcatttttc tttcctctat ttgttccgta tattaatcct
     9241 cggatcaatt atcgcaatca tcgtaaaatc gtagttattg atgggaaaat cggatataca
     9301 ggtgggttca atgttggaaa tgaatatcta ggattggtaa agaaatttgg ttattggcga
     9361 gacaatcatt tgcgtattta cggagaagcg gtgtatacct tgcaaaaccg ttttttgatg
     9421 gactggaatt ctcagcatac aaaagaagta ggctattctc ccaaattttt tccgtccatt
     9481 cactctactg gtgatatagc tgttcaaatc gtgacaagcg gaccagatac cgaacatgaa
     9541 caaatcaaaa tgacttattt gaaaatgatt tcgttagcca aacgggagat attgatccaa
     9601 acgccttatt acataccgga tggatcgatt catgaagcac tgaagttggc tcttctttca
     9661 ggcgtaaaag ttcatatcca aatacctaac aaaccagatc atcttctcgt ttactgggca
     9721 acgtattctt tcgctgcaga gctaatcgag tatggagcaa ggatcgaaac atatgagaat
     9781 ggatttattc atgcaaagac gatgattatc gatggtggca ttgtttcagt tggctcagca
     9841 aatatcgatg ttcgttcatt tcgcttggac tttgaggtca atacactggt ttatgatgaa
     9901 cgaatggctt cacgtgtaag aaaagctttt tttgaagact cgaaaatttc tacgcatctc
     9961 acaaaagaaa tgtatgaaaa ccgaggaata atcatcaaga tgaaagaagg attagcaaga
    10021 ttgatttcgc cgctccttta aaacataaaa aggacatgag aaccatcaac agatggagat
    10081 ctcgtgtcct tttctaacgg ggccatttct gagagtacgt cagaaatgga gaaagcgtct
    10141 cgcatgagcg aaattaactt tggggtatct cttgtttttt ggggaaagca agaaataagc
    10201 taatatatgc tattaaaaga gacacatttc aaagccagta tagcatagaa acttttaatg
    10261 agatagtttt ttttacaatt atgagaattt attttgttat ttaatgattt tgattcgttt
    10321 tcatcaagta atgaaaatga taaaataaac atactttagg attaaaggag atttctaatg
    10381 aaaaaactta aattattctt accaataata ggattagcta ttcttttatc aggctgttca
    10441 aagtggattg atcgaggaga atccattacg gctgttggct cttctgcttt acagccttta
    10501 gtagaaacag ttgctgaaac ttaccagaca gaaaatcctg gaaaattcat caatgtacaa
    10561 gggggcggaa gcggaactgg tttgtctcaa gtacagtcag gagcagtcga catcggaaac
    10621 tctgatttgt ttgcggaaga aaaatcgggg atcgatgcct cagcattagt cgatcatcga
    10681 gtagcagttg ttgggatcac tcctattgtg aacaaggata ttggcgtaaa agacatcagt
    10741 atggaaaacc tgaaaaaact atttacaggc gagatcaaaa actggagtga agtcggtgga
    10801 aaagatcagc cagtagttat attgaaccgc gcttcaggaa gcggaacccg tagcacgttt
    10861 gaaaaatggg tattggacgg agaaacagct gttcaggcac aagaacaaga ttcaagcgga
    10921 atggtgcgcc agatcgttgc cgacacaccc ggtgcaatca gctatgttgc tttttcttat
    10981 gtttccgatg atgtagatac gttgaatgtt aacggtgtag aaccgacaga tgaaaatgtg
    11041 acaacgaaca aatggacgat ttgggcatat gagcatatgt acacaagaga tcatccaaaa
    11101 aaactgacaa aagaattttt agactatatc ttatctgatg aagtacaaga taacatcgtt
    11161 ggagaactag gttatatccc tgtttctcat atgaaagtcg aacgtgattg gcaaggaaat
    11221 atcgttaaag agtaatcttt acacaaaatt tacaaactta gctaaacttt tacatgttat
    11281 taaaaccaaa ttgatgttca atttgtacac tgagtgagta tgttaaacct gattaagggg
    11341 gagtcatctg tggaagatgt gaaaaaagta ttattaacaa aatcaaaacg ttcaaaaatg
    11401 gaacagcgtg gaaaattcat cagtttttta tgtatcgcgc tgatcgtgtt ggttgtatta
    11461 tctatttttt attttgtagc tagtaaagga ttggccacct tttttactga taagatcaat
    11521 gtatttgatt ttctgtttgg tacagattgg aatccaagcc aaatcggagc aaatgggaaa
    11581 ccaatggtag gggcattgcc tatgatcaca ggttcgttta ttgtgacctt tttatctgct
    11641 atcgttgcca cgccatttgc cattggtgca gcagtattca tgacggagat ctctcctaaa
    11701 aacggaacga agatcttaca gccggtcatc gagctgcttg taggtattcc ttctgtcgtt
    11761 tatggtttta tcggtttatc agttatcgtg ccttttgtca gaagtatttt tggcgggact
    11821 gggtttggta tcttagctgg tacatttgtg ttgttcgtta tgatcttgcc gactgtcaca
    11881 acgatgactg ttgatgcatt aaaggcagtg ccacggcact atcgggaagc ttcattagcg
    11941 cttggtgcca caagatggca aacaatctac aaagtcgtgc tacgagcagc cgtgcccggg
    12001 attttaacag cagtcgtttt tggtatggct cgtgcgtttg gtgaagcctt agctatccaa
    12061 atggtcatcg gtaatgcagc attgatgcca acaggtctta ctactcctgc ttctacattg
    12121 acgtccatct taacaatggg aatcggaaat acaatcatgg gaacagttga aaataatgtg
    12181 ttatggtcat tagcgctgat cttgctattg atgtcattat tctttaatat cgtgatccgt
    12241 atgattggga agaaaggaga gctgaaataa tgaacgcaaa acgctcagat aaaattgcta
    12301 caggtgtttt atatatagtt tcggggatca tcgtattgat tttagcagct ttactacttt
    12361 atatcttagt tcggggaata ccgcacattt catggtcctt cttgaccgaa ccttccaaag
    12421 cctaccaaga aggcggtgga atcgggatcc agctatttaa ctcgatttat ttgcttttga
    12481 tcacgatgat catcagtttt cctatttctt taggtgcggg tatctatctt tcagaatatg
    12541 ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat tgaaatcttg agctcgttgc
    12601 cttctgtcgt tgtcggttta ttcggtttct tggttttcgt tattcaattc cagtatggtt
    12661 tctcgattat ttctggtgca ttggctttga ctgtattcaa cttgcctctt ttgactagaa
    12721 cagttgagga gtctttacaa gcggtccatt acacacaacg agaagcagga ttggccttag
    12781 gtttgtcacg ctgggaaaca gtgataaaag tggtggttcc agaagcgaca ccagggattt
    12841 tgactggtgt gatcctaagt tctggaagaa tttttggtga agcagctgca ttgatctata
    12901 cggctggaca aagtgcacct gcgttagatt ttacgaactg gaatccatta agtgtttcaa
    12961 gcccgatcag cattttccgt caagctgaaa cattagctgt ccatatttgg aagatcaata
    13021 cagaaggcac gatgcctgat ggtgtttctg tttcggctgg cgcttctgcc gtgttgatta
    13081 ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg caaacgatta taccgaaaaa
    13141 tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat aactggaatg aaacaaatgt
    13201 catcaccatg ccaaaagaga aagaagtagc actatatacg gatgatctac atgtctggta
    13261 tggcgacaat gaagcaatca aaggagtgga tctggaattt gaaaaaaata agatcactgc
    13321 actgattggt ccttccggct gcggaaaatc tacgtatctt cgttcgttga atcgaatgaa
    13381 tgatgggatc gctaatacga aaatcactgg gaaaatcatg tatcaaggcg tagatattaa
    13441 tgcgcctcaa gtggatgttt atgagatgcg caaacggatt ggcatggttt ttcaacgtcc
    13501 taatccgttc agcaagtcga tttatgacaa tattacattt gctttgaaac agcacggcga
    13561 aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc ttgaaacaag ctgccttgtg
    13621 ggaccaagta aaagacagct tggacaaaag tgccttggct ttatctggcg ggcaacagca
    13681 acgtttatgt attgctcgtg cgatcgcgat gaaacctgat atcttgctgc tagacgagcc
    13741 ggcaagtgct ttggacccta tttcgacagg aacggttgaa gaaacacttg taaacttgaa
    13801 agaagactat acgatcatca tcgtgacgca taacatgcaa caagcagctc gtatcagcga
    13861 ctatactgcc tttttctatt taggaaaagt aatggaatat gacaaaacta acaagatatt
    13921 taccagacca aaaattcagg caaccgaaga ttatgtgtcc ggtcattttg gttaagacgg
    13981 aggcctcaac atgacaaaag agattatttc atctaaagat cttcatctgt attatggaaa
    14041 gaaagaagca ctaaaaggga tcgatctaag cttcaatcaa ggggaaatca ctgcgatgat
    14101 cggtccttct ggttgcggaa aatccactta tctgcgttct ttgaaccgga tgaatgacct
    14161 gattccgaat gtaacgatca ccggaagtgt tttatataaa gacaaagaca tctatggtcc
    14221 aaaaacagat gttgttgaac taagaaaaga aatcggcatg gtattccagc agccaaaccc
    14281 atttcctttt tctatctatg aaaatgtcat ctatggattg aaattaaaag gggaaaaaga
    14341 taaaaaagtc cttgatcaag tagttgaaga aagcctgaag gcagcgtctg tttgggatga
    14401 tgtgaaagac aaattgcata aaagtgcttt atctttatcc ggtgggcagc agcagcgtgt
    14461 atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc ttattggatg aaccaacaag
    14521 tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg ctgttagaat taagagagcg
    14581 gtatacgatg atcatcgtga cccataatat gtctcaagct tcacgtattt cagataagac
    14641 ggcattcttt ttagatggtc acttgatcga atacaatgat acgaagaaga tattcatgaa
    14701 tccagaaaaa caagagacag aagattatat ttcaggtcga tttggataag gaggaaacgg
    14761 aatgctacgt acacaatttg aagaagaatt gctgaatctc cataatcagt tctacgaaat
    14821 ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt gcatacatca aacacgataa
    14881 aatcttagcc caagaagtta tcgaaaacga tataaatatc aataacatgg aaacacgctt
    14941 ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca gttacaactg atttgcgtat
    15001 gatcattacg gtcatgaaag ctagctcgga tttagaacgt atggccgatc atgcggtgtc
    15061 tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga ataccggaaa tcgaaaaaga
    15121 gatctccgat atgtccgatt atgtgaaaaa aatggtggac aacgtcctag ttgcttatgt
    15181 caaaacagac gaaaaagacg cacgtacgat cgccaatatg gatcaaagag tcaatgacta
    15241 tttcaatcga atctataatg caacaatcaa aaatatgcag gaaaatcctg agacagtcat
    15301 cagtggaaca gactacttga atgttgccag ttatctagaa cggatcggag actatgtgac
    15361 aaacatctgt gaatggatcg tctacttggc tacagggaaa atcactgagt taaatacgaa
    15421 ccacaatgag attttctaaa ctgaagctgt gattatgccg tttgtttagg aaaagaggtt
    15481 gtgaaatcag cggttgtgaa aaagctgttc tgcatcaagt aataagaaca gtcaaacaaa
    15541 aaatagctcc tcatattttt cgtttggctg gacttattac cgcagatgca agcttttgaa
    15601 caccgtttat tcgggaaagg gtttgtgaca aaacatgtca caaacccttt tcatcatttc
    15661 atagaaagga taaagaatgc aaaaaaaaat tagcatcatc actatatttt ttatttgtgt
    15721 gcttacttca tgggttattc agcatcaagc cgctttatat gaagtacctg tcatcgagat
    15781 agaaaaagta cgaataaaag aggatcatca attgattaca ggaaaactga tgaacacgga
    15841 tcaaaaagga gaatcgattt atttatcggt tccttatcaa aaaaatcaaa tagaaacagc
    15901 aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat ggagatgaat ggcagctagt
    15961 agcaaaaaaa aatgatggct ggcttttctt cttcacaagt atcttcattg gcgccatgtt
    16021 gcttattggt ggaaaacagg gtctatttgc tttaggaagt ctcatcatca atatcgtttt
    16081 acttttactt ttgttgctca tctttactaa aacagaaggg atttctctat taaatatatc
    16141 tatcctgttc gtcgttatag ggatgattat tagcatattg gctttagatg gatggaatag
    16201 aagcagtata atcaaaattt cagctgcagt tacgagtgtt tttttagcat tttttatttg
    16261 tcttttgaca atgaatcatt ggaaagacca aggactgcgc tatgaggaac tgaattatct
    16321 gacacgaccg tatcgttctg tttttctttc tagcgtactg attggaacag ctggaggaac
    16381 actcgatacc gttatcacag tcattgcgac attagaagaa ttgactaagc gacaaccaga
    16441 aataacagcg aaacagttat ggctttcagg aaggcaagtg ggtcgagatg tgattggctc
    16501 gatgaccaat atccttttgt tcgtctatat cagtgggtca gttccatcgc ttcttttata
    16561 tttaggaaat caatggtcgt tcaaagaaac gatagaacag catttatcat tggaattttt
    16621 acgcgtcatt gctggcagct taggaatagt attatcgatc cctatctcgg tcctcttttt
    16681 tctgactgta aggaggctga aacgatgaat gtacttatta tacttgttgg gattttagca
    16741 atcagcgtgc tacttattgg cggtacgcaa ggtatgtata ttcttttagg cttattcatc
    16801 aatttaggga tctttttcct gttgttattc ggttatcatc agaagtggcc gatactggca
    16861 ttgtcggtca taggattttt attgattgct gtggttatct tattttttat caacggttat
    16921 aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct ttttattttg tttcctacta
    16981 ttgcttccta tcactgattt tttagcaatc caaggtttta catcggtaga actagaagaa
    17041 ttatctggct tagataagac tttagcgatt gattttagat tacttacgag atcgttgctt
    17101 ctgattagtc ttagtggtgc tgttttagat gcgagtgtag caatttctag tgggacattc
    17161 gaagtatatc aggcaaatcc gcaattatct ttttaccaat tgcgccattc tagtttcgct
    17221 attgctaaaa aaatattggc gtcaacagta atgacacttc tttttgcatt catgggaagt
    17281 tctttagcgt tgatcctctg gtttattgat ttagacattc cttttcagca agtgatcaat
    17341 gaaaaatcat ttgttctaga atatacgatg gctattctta ccacattgtc cgcactactt
    17401 gttcttcctt taacctgcgt gactacagca tatctcttta caaaaaagaa agaacaactc
    17461 aagatggagt aaaagttaca acttaagtcc tatgacattt ttactgaatc agtgcataat
    17521 atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg aggcaatttc catgaaagaa
    17581 agagaacgta ttttagattt agtgaaaaaa ggaatccttt cgactgaaga aggcttagat
    17641 ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa tcaaaaaaga agcagacaag
    17701 gtaactgcta gtcataaaga aaaagatcag gcaagtcaat tgatcgataa acttgaaaac
    17761 ggcgaagaag aaatttctga gccagttgat ccaaaggaaa gagaaagaca agatcaagaa
    17821 aacttagaaa agatcttaga tgaactagca actgaagcga ataaaacttc ggcgcgtcta
    17881 gatgaagtaa atgctcaatt cgctgacaat aaggcagcac gcaatgagaa acaggaagca
    17941 ttgatgcagc tgaatacaaa agaagaatta ggcgagttga cagaagaaga attagcacag
    18001 cgtcagacgt tagaagcaga aatcaaagaa ttagaagcta gtggcgatac actgttagaa
    18061 gaacaattga aattagaagc agaattaaaa gatatccgta agaatcaatg gtctgagaaa
    18121 aaagaaacgt ttactgataa gtttgaattg ccagatgatt ggaaagatca agcaacagat
    18181 acactgaatc aagtaggcga aaaaatgtca gaagctggat cacagttagg caaattcttg
    18241 aagaaaacat tccaaactgt ttcagaaaca gtgaatgaca acatggaatg gaaagatgtc
    18301 agcttacgtg tccctgggat tgcgacaacg aaatttgagc atgaatttta ctatgaagct
    18361 cctgcagcaa gtatccttga tatcaaggca gcaaacggaa acgtgacgtt aaaaacgtgg
    18421 gacagcaacg atgtgaaagt tgaagctaag atcaaacttt acggaaaaat gggtgctgaa
    18481 ccatttgaag cattctctga acggagccag attgaggtta atgaagatca tatctccttc
    18541 caaattccaa ataaacgtgt gcgtgcagac cttgttttct acttacctaa acgagtatac
    18601 gaccacgcag ctattaaact tctaaatggc aacatcatga ttgaaacatt ggaagctaaa
    18661 gatatttata caaaatctac gaatggtaac atcatcgtgg accaattaac tgcaacgatg
    18721 ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa acgggaacat cttagattct
    18781 attatcgaaa cagtaaatgg tacagtgaca tttggtgcga cagcagaaaa cctttctgtg
    18841 tctttggtaa atggtgatgt acgtcttacg attaaagaag ataatttgaa aaaagtagaa
    18901 gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag atggcatcgg tttggaagga
    18961 catgctaaaa caagtcttgg aagtatcaac agccgtctgt ccaattacga agtagttcgt
    19021 gagaaaaaag aacggacaaa ccaaatgttg caattccgtc gtgtttcaga tggtgaaatc
    19081 gcgcaagtgc aactttcaac gacaacagga agtatctatt taaaagatac agataaataa
    19141 agaggttgtg aaagagatgt tcagccttga gcaataagaa gcgattttgt aaaatagctt
    19201 cccctatttt acaaaattgt gccttattgc cgatagtcta tctcttgaac accgtttacc
    19261 aagagattat gaacctgcca attatcgccg agtcacaagg aataatttca gaaaaagctc
    19321 gtcacgtttt atgaatatgg taatttgtga ccgagaattt tgcagaaaga caccgattaa
    19381 gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt agaacagagg tgagacatga
    19441 tgaaaaaaaa gttgacaaaa tctaataaaa atgttgtttt gacagggact ttagcaggaa
    19501 taggagaata catcgggatt gatccgactg ttatccgtgt gctttatgta ttcctgagct
    19561 tagttgcttt tggaagtcca attattttat acattttatt agctttgatt atcccatctg
    19621 cgcgttctgc acgaacaaac tatggacata acaatgcata ttatgaaaaa aacaattatc
    19681 gtgaacaaac tcgtgaacaa acaaaaaaac gcaaagaagc ggaaaaaatc gatgatgacg
    19741 aatggagtga cttttaatgt cgtactttca acgtatttta gttaatacgt taacattcat
    19801 atcattggct gttctttttc ctgcgatggt ccatgtgagc agtatttgga cggccgttct
    19861 tgccagcttc gtattgtctg tgttgaatat gtttgtcaaa ccgattttga cgatactgtc
    19921 cctgcccttt acattagtca cattgggatt gtttacgttt gttatcaatg cagcgattct
    19981 taaaatgacc tccttttttg tgggagcaga acattttagt ttttcttcat tcggtgcagc
    20041 gttgttagta gctatcttga tgtcgcttgt caatgtaatt gtcagcgaac ataacagtag
    20101 aaaataaaag aaagctgtgc cgaactatcg gcgcagtttt tttatttaga atcaggaatg
    20161 cgttttctat tacattggta attaaataaa aacgtttttt ttaattggta gtacttttgg
    20221 ctagacaatg ataaaatgat agagaacact ctaatagaaa tgaggtagca tgaatgtcag
    20281 aagttgtaaa agttagccaa ttagtgaaaa aattatcttt ggaaatcgtg acaggcgacg
    20341 agaaaagctt agaccgtgtc atcgtaactg gggacatctc acgcccagga ctagagttga
    20401 ctggttattt taattattat tcccataatc gtttacagct atttggcagt aaagaaatca
    20461 gttttgcgga acggatgatc ccagaagagc gtttgatcat catgagaaga ttatgtagtg
    20521 aagatatgcc cgcatttatt atttcaagag gattaccagc tccagaagaa atgatccagg
    20581 cagcaaatga aaatgggata gctgtactgc gttctcccat ctcaacttct cgtttattag
    20641 gagaattatc cagttattta gatggtcgcc ttgcccctcg aacaagtgtc cacggtgttt
    20701 tagtggatgt ttacggatta ggtgtactga ttcaaggtga cagtgggata ggaaaaagtg
    20761 aaacagcgtt ggagttgatc aaacgcggcc atcgattgat cgcagacgat cgagtagatg
    20821 tttatcagca agatgaatta acggtgatcg gagaaccgcc taaaatcctg gaacatctga
    20881 ttgaaatccg aggaatcggg atcattgatg taatgaatct ttttggtgcg agtgcggtac
    20941 gtggctctat gcaagtccag ttggctgttt atctggaagc atgggcaaaa gataagaaat
    21001 acgaccgtct gggctctgaa gacacgaccg ttgaaatcgc ggaagtgggc attccacaaa
    21061 taaaaatccc tgtcaaaaca ggacgaaacg ttgcaatcat cattgaagta gccgcgatga
    21121 atttccgtgc acgtacaatg ggctttgacg caacgaaaac atttgaagaa agattaagtc
    21181 gcttgattga agaaaactca ggcaacgact agcaaggagt gacaatatgt tagcacaaat
    21241 caatcgagtt gcttttgagc ttgtagggat tcctatctac tggtatgcat tgatcatcgt
    21301 atcaggtatc attatcgcta tgtggcttag cagcagagag gctgttcggg taggattaaa
    21361 agcagatgat gtgacagatt ttatgttgtg gggattgcct atctccatta ttggcgctcg
    21421 cttatattat gttttatttg atcttcccca gtatatagct gatccaatcc aaatattcaa
    21481 tatccgttca ggaggcttag ctatttatgg cggactgatt gcaggcgcga tcacgctgta
    21541 tttttttact aaatatcatt ttatttcttt gtggactttt ttagatatcg ctgcaccaag
    21601 tgtattatta gcacaggcaa tcggtcgttg gggaaatttc atgaaccacg aagcatatgg
    21661 accagacacg acgcgaagct tcttagaaag tctgcattta ccgcaattca tcattgataa
    21721 tatgtatatc gaagggatct atcgtcaacc aacatttctt tatgaatcac tttggagttt
    21781 gattggattt attttgcttt tgtttttacg aaagaaacca caccttttga aaaaaggcga
    21841 agtcctttta tgttatctaa tgtggtatag tttcgggcgt ttctttattg aaggattacg
    21901 tacagacagt ctttactttt taggtgtgat ccgtatttct caattattat ctgctgtgtt
    21961 atttgtcggt acattgattc tatttatttg gcgcagaaaa aaaggaaatt taccggatta
    22021 tgaccgctca ttaggaaaaa atcaacaaat tatctgattt ttagagcgtt aaaaatgaaa
    22081 aaaatttcag gagtggtaaa atgaattata gagtagcggt tttaggacca ggctcttggg
    22141 gtactgcttt ggcacaagtc ctcgccgaaa acggacatga cgttcggatt tggggacatc
    22201 atgcttcaca agtagaagaa atcaacaccc aacacacgaa tcaacgttat ttaccagatc
    22261 attatctacc cgattccatc caagcattca ccaatatgga agatgcagta aaagacgcag
    22321 atgcggtgct attcgtcatt ccaacgaaag caatacgttc tgttgcaaaa gaactagtgc
    22381 ctaaattgaa tacaaagcca gtgatcatcc atgcaagtaa aggattagaa cagggaacac
    22441 ataaaaggat ctctgacatc ttgttagagg aaatcccagc agaaaaaaga aaagctgttg
    22501 ttgttctttc tggaccaagc catgcagaag aagtagcaaa acatgacatc actacgatca
    22561 cagctgcaag tacagatgca aaagcagcaa aatatgtaca agagctgttc atgaataatt
    22621 attttcggat ctataccaat cctgatgtga tcggtgtaga gatgggggca gctttgaaaa
    22681 atattattgc tattggtgct ggcgctattc acggcttagg gtttggcgat aatgcaaagg
    22741 cagcaatcat gacgcgcgga ttagctgaga tcagccgttt aggtgtagca atgggtgcca
    22801 acccgctgac ctttatcgga ttaagcggaa taggcgatct agtcgttact tgcacaagtg
    22861 ttcattctag gaactggcgc gcaggcaact tgttaggaca agggcagccc ctagaagagg
    22921 tgctagccaa tatgggaatg gtcgttgaag gtgtgaatac tacccaagca gcgatggaac
    22981 tagcccaaat gatggaaatc gaaatgccca tcactcaaac aatctatgaa gtgctgtatg
    23041 aacaaaaaga aatcaaaaca gccgcaaaag aaatcatgtt acgtgatgga aaaaaagaaa
    23101 atgaattctc attaacaatt ttttgaagga gcgaatgtat atgaaagtta aaaaagcagt
    23161 tattccagca gcaggattag gaacaagatt tttaccagca acaaaagcta tggcaaagga
    23221 aatgttgcca attgttgata aaccaacaat ccagttcatc gttgaagaag cgttgaaatc
    23281 aggtatcgaa gacatcttga tcgttacagg aaaagctaaa cgaccaatcg aagatcactt
    23341 tgattcaaat attgaattag aaacaaacct aagtgaaaaa ggaaagaccg aattattgaa
    23401 attagtagaa gaaacgacag atgtcaactt gcatttcatc cgtcaatctc atccaaaggg
    23461 tttaggacat gccgttcttc aagcaagagc ctttattgga aatgaaccat ttgtcgttat
    23521 gctaggcgat gatataatgg aagacgaagt accattgaca cagcaattga tcgatgacta
    23581 tgagcgtaca cacgcttcta cgattgccgt tatgcaagtt cctcacgatg aaacttcaaa
    23641 atacggaatc atcaacccag gggaagtact ggaagatggg ttatacaatg tcaaaaactt
    23701 tgttgaaaaa ccagatccaa gcaaagcacc aagtgattta gctattattg ggcgttactt
    23761 attgactcca gaaatctttg atgtattatc agaacaacaa ccaggtgctg gaaatgagat
    23821 ccaactaact gacgcaatcg atacgttgaa taaaacacaa cgcgtatttg cccgtaaatt
    23881 cacaggtaaa cgttatgacg ttggtgacaa atttggtttt atgaaaacaa gtattgaata
    23941 tggtctgaca catccagaag taggggcacc tttgcgtgaa tacatcatgt ctttaggtaa
    24001 aacattagaa gcagaaggaa gcaagatcaa tcataatcca aaaggaataa cagaaatcaa
    24061 agaagaaaag aaaaaataat ctttttttct aacgagcttc agcggctaat tgaataaaaa
    24121 gtagttgaag ctcgttttta tttgtttaaa aaacacgaaa atgctatcct tatagaaaag
    24181 agagacaaga agggagtttg atcatgtcag gaggagaaat tgcaggattg atcgcagcaa
    24241 tcgcctttgc gatacttgtc gttttcatcg ttcgcgtatt gttgcaagtt tccaaaactt
    24301 tagaaaaagt agaccagact gtcgcagaag caaatacgac aattgagatc gtcacacaag
    24361 acgtagatct tttatctaga caagtagaag gcttgctagt caaaagtaat gagctgctct
    24421 tggatatcaa tggaaaagtc gcaacgatcg atccattgtt cacagcagtt gctgatttaa
    24481 gtgaaagtgt atcggaactt aatcattcta gtaaaaacat cgtgacgaaa gtcggcggaa
    24541 tcggaaaaac gacagctaaa gcgactgttg ccggaaaagt cggtggaaca gcgatgaaat
    24601 tttttaaaaa caaaaaacat actgaaacga caggaggaaa ataagaatgg ctaaaaaagg
    24661 tggattttta ttaggtgcag tagttggagg tacagccgca gcaatcgccg cattattgtt
    24721 agcacccaaa tctggtaaag aacttcggga tgaattggct gctcaagcag atgatttgat
    24781 ggacatggct tcagattaca cagatatggc aaaagaaaaa tcatctgagt taacaggtat
    24841 tgcaaaagaa aaagcgcaag gtctttctca acaagcaggg gatctagcag gcaatgttaa
    24901 gcagaaggta agtgaatcag cagaggaagc aggaagtgaa tcagatgata tcttgttaaa
    24961 cctcaaagaa caatcagctg atttatctaa tcgtttcaaa aaaacagcag aagatctgaa
    25021 aaagccagca gacgaattag gtagtattgc taaagatact tcagaagata tcatgatcga
    25081 tgtaaaagat gctgcagata aagcaggaga gacggtcaat gaaggaatgt cagaagcaaa
    25141 accagttgca gatgacacaa aagaaatgtt gaaaatggca aaagaagatg caaaagaagc
    25201 taaaaaagaa atgaaagata caatgacaga ttcgactacc aatcactaag attactcatt
    25261 tctagaataa aaaaggtgag agcggaccag ctctcacctt ttttattttc attactctaa
    25321 aacaagtaat tcttttggtg tagtcgtgaa tggcacacaa ccatcacttg tcacatacac
    25381 acaatcttcg atacgtaccc ctactttttc aggaatatag attcctggtt caagagagaa
    25441 gcacatccct tcttcaatca ctaagtcgtt tccttctaca agagaaggga attcgtggac
    25501 ggttgttccg attccgtgac ctaaacggtg attgaaatat tcgccgtagc catagctgtt
    25561 gatcacgttt cgtgcgattt gatcaagttc acctgctgtc acacctgggc gaactgcctc
    25621 ttgtgcggca agctgtgctt caagagtgat gttatagatt ttttcttgga actcagttgg
    25681 tttttgataa gcaactgtac gagtagcatc actgcaatag ccattccaaa taacgccaag
    25741 atcgaataac actagttcat ttggtgaaac cttcgtgctt cctggtgtac catgagggct
    25801 tgcagcattt gctcctgcaa ggactaatgt gtcgaaagac atttgtgaaa caccttgttt
    25861 ttttaattgg tattcgattt ctgcgataat tgcttgttca gttacgcctt cttttactgt
    25921 tttgaagcca atttcaaagg caacatcggc ccatttacca gcttcgatca gtttgtcttt
    25981 ttcttctacc gttttgatca gttgcagacg ctggataata gaagttagat cggtgtaaga
    26041 atctgtacct gtcaagatat cagagatagc atcgaagcga tcgacagtca gagcattctt
    26101 ttcaatacct acattaccag gttgcccata gcgtgcgcga agctcttttc cgatgatctc
    26161 ccaaggattt tcgctgtcta agtagccacg tacatcgtac gcccagctgc tgttttgagc
    26221 atcttcaact tccagagcag gagtgaaaag gaaaggatct tgttccagag ggataaaaag
    26281 agctagtaca cgctcgtgag gatcactttc atatccggaa aagtaagcga tgtggccagg
    26341 gtcgctgata taagccaatt ctacttggtg ttgtgccatc cattgacgta gttcattgat
    26401 ttttacgtta ttcatttggc aacctacttt ctaaattaat tttgtattca ataattgtac
    26461 cactgattga ttaaaagacc aacgttctta aaactgaaaa aagtaaaaat tttcatgaaa
    26521 acagagataa aacggttgca attgttgaga ttatttgata tcctatttta gaaatgtaag
    26581 acgtattttc agaaaacata taaaatatga ataggagaac acaaatggaa aagcaaacaa
    26641 tcactatcta tgatgtagcg agagaggcaa atgtttcgat ggctactgtt tcacgtgtcg
    26701 taaatggcaa tccaaacgtc aaaccagcaa caagaaaaaa agtcttggaa gttatcgatc
    26761 gcttggatta tcgtccaaat gcagtcgcac gtggcttggc aagtaagaaa acaacaacag
    26821 ttggggtaat cattcctgat gtcagcaata tgttctttgc ctctttagca cgtggtatcg
    26881 acgatgtggc aacaatgtac aaatacaata tcatcttagc aaactcagat ggcaacgatc
    26941 aaaaagaagt caatgtattg aataatttat tggctaagca agtggacggc gtgatcttta
    27001 tgggtcacca tatcacagac gaaatccgtg gagaattttc acgttcaaaa acgcctgttg
    27061 tactagctgg ttccattgat ccagatgaac aagtaggtag cgtaaacatt gattatgtgt
    27121 cagcaacaaa agatgcagta aataaattag caaaaagcgg caataaaaaa atcgcttttg
    27181 tcagcggtgc gctgattgat cctatcaatg gtcaaaaccg tctaaaaggc tataaagaag
    27241 cattaagcga aaacaatcta ccatatagcg aaggattgat ttttgaggca caatacaact
    27301 ttaaagatgg tctgtctcta gttgaccgga tccataatag cggtgcaaca gctgtctatg
    27361 tttcagacga tgaattagcc attggtatct tggatggttt attagaccgc ggcgtaaaag
    27421 taccagaaga attcgaaatc atcacaagca acaattcttt actgacagaa gtagctcgtc
    27481 ctcgtttgac aagtatcaca caaccattat acgatttagg agcggtatcc atgcgcctat
    27541 tgacgaaaat gatgaataaa gaagaagtcg aagaaaaaac gattatcttg ccttatggaa
    27601 tcgaagaaaa aggatcaact aaataataaa agatcaatca tttaaaggtt gttccaaaag
    27661 tggtcaactt ctggaacaac gtttatacga gaaagggggt tatgacaaaa ttttgtcata
    27721 accccttttt ttttgtgact ttaaaaaaag agaaaatggc ggaaacaaac cttacgtcca
    27781 tcgtatacga cacaatctgt gcgtagatgc tacattttgt tcactactat caaaattact
    27841 ttaaaatgaa aacgtgataa aaaagaaagt agaaaagtag ataagtaact ctaaaaaatc
    27901 caaaaaaatc caggctcgat tgcgtactta tcttaaagga gaaacaatcc atcatatata
    27961 acccgaaagg agacttctat ttgcctaaga aaagacgaaa gaccatagtc atcattttga
    28021 ccattcttac tataggaatc ggagggaaat tttacatgga caaaagagaa atgcaaagac
    28081 agaaagattt actcgcagta gaaaaacagt ccgtcaaagt tctgaaaaat acctttgcgg
    28141 atatcaagga agtgaagatt gaaaaatctg ctcgaaatga aatgacgggt tcataccgga
    28201 tagttattct aatgactaat aaacaagatc aatcgattta tttttcttat agtttttgga
    28261 aagaaagaaa tgagatagga agttacggaa tagttgatga aaaaaagcaa aaagaaggaa
    28321 atacgctcaa taaagttaaa gtaacttact ctaatggaaa tgaggaaagt atttaatgat
    28381 tactgacaaa caatatagtg aattaagtga tgctgtttac tggctagatc ctaaacatag
    28441 agactatgta cctgaaatgc aagaaaattt aagttttaaa attaacggca ctatgtataa
    28501 aatcctaaaa atcaaaaact ccttcgacgg catgcaggcg atggctgtag cgccaatcgt
    28561 ccatagcaaa ttggagaaaa atttcaaaaa taaaaaaatt cctgcaaact ttcgcgtact
    28621 taaataatga aggtgtcaat acttacataa aaagaaagga ggcacgttct tgtcaaggaa
    28681 aataagaaaa ctcagcttag ccattttatt aatcgtcata ttagggatcg gagggaaagt
    28741 ctatatggac aaaagagaag cacaaaggca gaaagattta ctcgcagtag aaaaacagtc
    28801 agtcaaagtt ctgaaaaata cctttgcgga tatcagggaa gtgaaggtag aagaatttaa
    28861 aaaaaaccct gttaccggaa gttatggtgc acttgtgaca atgacaaata atgaagggaa
    28921 atctgtttat tttgattata gtttttggaa ggagagaaat gaacttggtg gttttggcat
    28981 tgagaacaga gcggtacaaa agacaggagt cactataaaa agagtaaaag taattttttc
    29041 taacggacag gaggagttag tatgacaata aacgataagc attacaatga tattagtgag
    29101 aaggtttatt ggctagatcc caaatatccc cgctacaatg agggatacaa aaagaactct
    29161 gttaaagaat ttgctggtat ggaatttcaa atcctacaaa tcaaagactc attggacggc
    29221 atgcaggcga tgtctgtagc gccaatcaag aacggtaaag tggatctttc tcaagtagtg
    29281 attgcttttg caggaacgaa cttaagtgat tggaaagatt tggaaacgga tgcaagaagt
    29341 atcatgaatc tctacgggaa tagaccagga gcaagttttt ctgaaaaagc aacaacaaac
    29401 aagattttat ccgttagtgg attgaaaaca gcggggcaaa tcaattcagc gattgcattt
    29461 gctaatgaag taaaaaagga atatggagaa gctgctattt ctactaccgg ccattcatta
    29521 ggtggattct tagcattata tgtagcagca gagaaacaat ggaaaaatgt cggcttcaac
    29581 gggccggacc cgtatgatct cttatcacca gaagcaaaaa aatgggtgga agaaaatccc
    29641 ggtatgctca ccaattaccg aaatcacgcc gattttatcg gtaattttgg tggtaatggc
    29701 acaggtgctg aagttcggat cagtatggaa acagcattct tgaatctgct tgcttcccac
    29761 agtttaacct cttggaagtt tgatgaaaat ggaaagctga ttattcctga aaatgaaaac
    29821 aatgttaaag ctcgaaaaca acagaaagaa aaccaagcga cccagttgtt ttcctatcag
    29881 ttgcaagaac tgaacaacat acgtaaaaaa ctacgattaa gtggcggcca gctgacagtt
    29941 aatgaacaaa tttacttaga tgacacacaa gctaggttag tagtggaaaa gttatcggca
    30001 aaaatgaaaa gcgctatgga agaagttatc cgcatcaatc aaagaggaat ttttgagcta
    30061 gagaaaaaat ggcaagaagg cttgcggcaa gcacgtgttg tagccccttc gttgactgaa
    30121 caggaaatca tagaagcatt agctagtgtg ggcgccacaa aagaaaactt agtcgattct
    30181 gcgaaagaag tctatctgct acgaatcaat aaggcaagac gtatggggga ggcctttgat
    30241 acattggcga aggagatcca agacaaaatc aatgaaatcg tggcacgaga tcgagaatta
    30301 gcacagcaat tcaattaaag gagaagtaga tgaaactaga agaattggaa agagattatc
    30361 gaagtgaacg aagacaatta gaagctcaag aagatgaatt gatccacttt aagaatcaag
    30421 gattacgatt gatggagcaa gaggagcagc gactgcatca tgtattaaag aacttagctt
    30481 tagataatga gcctctgttt caagcaagac gaagttattc gaattatcag gagcattatc
    30541 aacaagccct acaagtagaa cgacgaaaac tctatcagca gcaagatgaa ctagaacgtt
    30601 cctatcgtca aaaaatcaga caaataacta tggagaaatc atcttatgaa tgaattttta
    30661 atgcaattac taaaagaatt agagaaaaat ctacatcgat caaatgtacg ttcagttggt
    30721 atggggatat ctcagcaaat ttgggcagaa aaacgattga cagatcaaaa ctaccaaatg
    30781 actgcagaag aagctgctca agaattttct aaccgacatg tacacttgaa aggatcagta
    30841 aaagggcaat ttgcagaaaa actaactgaa ttattccaaa gacaagaaaa gaaattaaaa
    30901 cagtttcgtt agtaaagtta tatagaaaaa ttttgtgaaa atggttt
//
LOCUS       DACEUF010000028        29740 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.029, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000028 DACEUF010000000
VERSION     DACEUF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.029"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(192..662)
                     /locus_tag="IXG20_001217"
     CDS             complement(192..662)
                     /locus_tag="IXG20_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ1609426.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            992..2632
                     /locus_tag="IXG20_001218"
     CDS             992..2632
                     /locus_tag="IXG20_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ1609427.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2786..4108
                     /locus_tag="IXG20_001219"
     CDS             2786..4108
                     /locus_tag="IXG20_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1609428.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4176..4607)
                     /locus_tag="IXG20_001220"
     CDS             complement(4176..4607)
                     /locus_tag="IXG20_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1609429.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEA
                     KKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4672..5508)
                     /locus_tag="IXG20_001221"
     CDS             complement(4672..5508)
                     /locus_tag="IXG20_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ1609430.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(6077..6382)
                     /locus_tag="IXG20_001222"
     CDS             complement(6077..6382)
                     /locus_tag="IXG20_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ1609431.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(6407..6838)
                     /gene="ruvX"
                     /locus_tag="IXG20_001223"
     CDS             complement(6407..6838)
                     /gene="ruvX"
                     /locus_tag="IXG20_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ1609432.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(6835..7101)
                     /locus_tag="IXG20_001224"
     CDS             complement(6835..7101)
                     /locus_tag="IXG20_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ1609433.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(7356..8081)
                     /locus_tag="IXG20_001225"
     CDS             complement(7356..8081)
                     /locus_tag="IXG20_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ1609434.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(8442..8512)
                     /locus_tag="IXG20_001226"
     tRNA            complement(8442..8512)
                     /locus_tag="IXG20_001226"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8478..8480),aa:Gly,seq:ccc)
     gene            8629..9381
                     /locus_tag="IXG20_001227"
     CDS             8629..9381
                     /locus_tag="IXG20_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ1609435.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            9397..9876
                     /locus_tag="IXG20_001228"
     CDS             9397..9876
                     /locus_tag="IXG20_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ1609436.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(10127..11707)
                     /locus_tag="IXG20_001229"
     CDS             complement(10127..11707)
                     /locus_tag="IXG20_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ1609437.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(11700..12443)
                     /locus_tag="IXG20_001230"
     CDS             complement(11700..12443)
                     /locus_tag="IXG20_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609438.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(12619..13395)
                     /locus_tag="IXG20_001231"
     CDS             complement(12619..13395)
                     /locus_tag="IXG20_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1609439.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(13643..14479)
                     /locus_tag="IXG20_001232"
     CDS             complement(13643..14479)
                     /locus_tag="IXG20_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ1609440.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(14490..15419)
                     /locus_tag="IXG20_001233"
     CDS             complement(14490..15419)
                     /locus_tag="IXG20_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ1609441.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15611..15964
                     /locus_tag="IXG20_001234"
     CDS             15611..15964
                     /locus_tag="IXG20_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1609442.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(16038..16694)
                     /locus_tag="IXG20_001235"
     CDS             complement(16038..16694)
                     /locus_tag="IXG20_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1609443.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            16920..18854
                     /locus_tag="IXG20_001236"
     CDS             16920..18854
                     /locus_tag="IXG20_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1609444.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(18940..20163)
                     /locus_tag="IXG20_001237"
     CDS             complement(18940..20163)
                     /locus_tag="IXG20_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ1609445.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(20157..22055)
                     /locus_tag="IXG20_001238"
     CDS             complement(20157..22055)
                     /locus_tag="IXG20_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ1609446.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(22049..23167)
                     /locus_tag="IXG20_001239"
     CDS             complement(22049..23167)
                     /locus_tag="IXG20_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1609447.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(23250..24083)
                     /locus_tag="IXG20_001240"
     CDS             complement(23250..24083)
                     /locus_tag="IXG20_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1609448.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(24076..24993)
                     /locus_tag="IXG20_001241"
     CDS             complement(24076..24993)
                     /locus_tag="IXG20_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1609449.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(25220..26344)
                     /locus_tag="IXG20_001242"
     CDS             complement(25220..26344)
                     /locus_tag="IXG20_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1609450.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            26527..27435
                     /locus_tag="IXG20_001243"
     CDS             26527..27435
                     /locus_tag="IXG20_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1609451.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            27576..29189
                     /locus_tag="IXG20_001244"
     CDS             27576..29189
                     /locus_tag="IXG20_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ1609452.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         9192 a         5825 c         5077 g         9646 t
ORIGIN      
        1 tgacttcaca gtctcttcct taaacggtgt tcaaaaactt gcatctgcgg taataagccc
       61 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcagg
      121 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
      181 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      241 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      301 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      361 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      421 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      481 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      541 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      601 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      661 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      721 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      781 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      841 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      901 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      961 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
     1021 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
     1081 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1141 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1201 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1261 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1321 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1381 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1441 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1501 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1561 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1621 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1681 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1741 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1801 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1861 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1921 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1981 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     2041 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     2101 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2161 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2221 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2281 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2341 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2401 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2461 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2521 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2581 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2641 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2701 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2761 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2821 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2881 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2941 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     3001 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     3061 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3121 cagcaatatg ggctttttgg acttttatat tgctgccttg attactggta gcatccttgg
     3181 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3241 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3301 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3361 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3421 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3481 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3541 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3601 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3661 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3721 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3781 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3841 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3901 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3961 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     4021 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     4081 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4141 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4201 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4261 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc tacgccagtc
     4321 atcaaagcct ttccgatgcc tcttttttca gcatatttag acacagcaag tgccataata
     4381 ttgaacattg gatcgctaaa aatcgattca taggactccg cttgtacata gccagctgct
     4441 tttttagaag taggatcttc ataaaccaag aaaaaatttt tctctggctc cttcagcagt
     4501 ttcttcattt gtctcaccgt aacggttaat ggtatatcat accctaattg atccgcattg
     4561 atttcacgta gtgcttctgc atcttctaaa cgcatttccc gtaacatcca aatcaccagc
     4621 caatttctga ttttttaaaa tgaatttttt tatcatagta tactcttttt ttcatttaga
     4681 acaatatgtt tcagtgattt tgacttgcgt tagtctagat tctcctaatg tttgtaagat
     4741 gacaaaggca ttgtaacaat tgaccaaaag aaccacgagg acaattactt gaaaaataaa
     4801 ggtcactttt tttggagaca aaaccctgct aaatcttgct ccccacaaac ctccaataac
     4861 agcagcgggg ataacataaa acaacataga taaatcaaag cgcataaagc tacttgttaa
     4921 agcaatcgtt accaattttg ccagttgaga aaagaaaatc gtagatagag aatatagtgt
     4981 tgcttctttt attggcaatg caaacatcag catcaacaaa gaaacattga ctggtccacc
     5041 accaatacct aaaaaactag ctaaaaagcc cagaaccccc ccacatataa ggtaccaagt
     5101 ccatgacgtc aaatgaaatt ttggctgatg atgcttggtg tagaagaacg caaaaaccaa
     5161 ggtcaatacc gtcaaaaaaa tttggatcag ctgcacatgc ttctcgtttt caaataactg
     5221 taaaaaaact tcgaatacaa tgttgccgac tactcctcca acaacagctc cactagatac
     5281 ccaaccgaca atctgccaat tcagtgattt cccgcttgat acttgtcgca cagtcgaaac
     5341 aatggacatc gtgaataccg caactgttga gtaaaaagaa atccctgcaa cagaatgggc
     5401 gccgataaga tcaagaatcg gtttgatcaa cacgcctcca cccatcccgg aaatagcgcc
     5461 aactgtgttc gccagaacga tcaccacaaa ataaatcaga ccaatcatct tgtatttcct
     5521 ttctcttgtt tatatctttt agcgattgtt actcttttct tttattatac agtaaaagaa
     5581 acaaacgagg cttcgaaagc ttttctactt tttaaggaaa tttagtgcta aacgaaaaga
     5641 ggttgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagctcctc
     5701 ataattttcg tttggttgaa cttattaccg cagatgcaag cttttgaaca ccgtttaaga
     5761 aagaggtcgt gagcctgccg ttcagctccg agtcacaagg aacaatttca aaaaacagct
     5821 tctcatgttt gttgaatatt gtgacttgtg accgaggagt tggcaggaga acaccgttta
     5881 aggaagagac tgtgaagtca acgttttgac ctgattatta gagaaacaaa tggcaaaata
     5941 gttcttcata tttcgccgtt tttcgagcga atgccgaggg gcagctgatt gaacatcgtt
     6001 tatttggaaa aagggattgt gacaagacgt ttgtcacaat cccttttttt atatcttatt
     6061 aattcattct tttgttctat tcttcttctt ccgccatgaa tgtattgaat acttcttcaa
     6121 tcatgtccca ttctgcgtct gtttcgattt gttctaactc gccttctgtt ccatcttcgt
     6181 tttcgatgta tgcataagct tgtaattcta catcttcatc ttctgaaaca ccagcaggat
     6241 ataaaagaac atagttacgt ccaaattctt cttgtccgtc aactgttagt aggatttcat
     6301 ataatgtttc atttccttgg tcatccacca atgtgatgtg ttcgtgtcct tcatggtcgt
     6361 gggaatggtt atgttctgtc ataagattac ctcttttctt ttattattaa aataattgct
     6421 tacctgagcc gtctaaataa ttttgtaaga tcatcacagc tgctaattta tcgatgactt
     6481 tttttcgttt ggctcttgaa gtatttgcct gctctactag catacgttct gcctgaacag
     6541 tggttaatcg ttcatcttga taatcaactg gcaaggagaa tagttctttg atcttttctc
     6601 cgtatgacat ggaagcttct gcccttggtc cgatggaatt gttcatattt ttaggtaatc
     6661 caactacaaa ccggtctact tcatattgtg ctactaattc cttcaaccga tcaaagccaa
     6721 actcttcttc tgcttcattg atccgaatga tctctactcc ttgagctgtc cagccaaagg
     6781 ggtcactgat agcaacacct actgtcttag aaccgacatc caagcccatt actctcataa
     6841 attcactcca tgatttgaga ggtagtcctt gacaattact tccatgatct catcgcgttc
     6901 gtgacggcga atcaaatttc gagcatcacg ataacgagga atgtaggccg ggtctccaga
     6961 aagcaagtag ccgacaatct gattgattgg attgtagcct ttttcttcca aagcccgata
     7021 aacaatcgct aatgtttcgc tcacttcttt tttgcgacta tcgtcaaaac caaaacgtac
     7081 ggtttcatct gtaaaaccca tgattctaca cctctttccc gcaaggtaat tccagcttac
     7141 cttctacaac cttattttac tcgaagaaag ggaaaactac aaacaaatat tccaaacttc
     7201 cgaataaatt aagaaacact atttgtatgt ttttttcctt ttttctgtag ctttatcaaa
     7261 agtactcttt tcataaagta tacgctatat ttctttgaat gtatgttttt acacaatttt
     7321 ttcataatat tcataaaaaa ttcgaatagc tgctactaat tttttgtttt tgttgcttgt
     7381 cctgtctcat aattgatcgt gtagccatct tgaacattgt agataaagac attgaattca
     7441 atttttttgt cttcaatgct ttctgcttcc aattgcactc ccctggctac cagttcattc
     7501 ccctcaaaat gaggagtgac acggtagcgt acatgatgat ttgtttcttt gatatatgcc
     7561 gcaatatcat tttcgtgagc aagcatgtat ggagcattca acgaacgtgt ccctgtcatc
     7621 aagtttttaa tgttgttatt ttgaccagtc agctggaaac caattaaatg gctgcgatta
     7681 tataaccatt gcccgtcact taatttcttt tgtttccaac ctgtcgggtc aatatacaaa
     7741 ggctctctct tttcagtagg gaacaaatct tttccaagca tagcattggc ttgtcctact
     7801 ctgttcaatt tatcaatatt tgaatagctt tcccatgttc ctttggaaag gctaagatcc
     7861 tcttttgtaa attccggctg attcccattg atttctattt cttgatgttt tccatcatat
     7921 tctggcacat ccgctacttg agaagcttgt ggtgtggaat caaaaatagt tgtaatctgc
     7981 tgaaagatat ctgaatcttt ttgtgtggtg gaaacaccga atgcccctgc aatcacagca
     8041 gcgacgatcc ccacgatcgc cttggttgtt tttttgctca tttctttact cctttactaa
     8101 cagctcccta aatcttaaca tatgttcccc tataaataaa tactagaaag aaatagaaac
     8161 agcaaaaaat aaaacctgtt ttaagatggc ttaaaaatac aaaaaagaga cggattcctc
     8221 gtctctaatc agtcaccact ctatctgtaa ggtatttatt attattcaaa aattttcttc
     8281 atactcaaga caatacactg atttcacaac cgcttggtaa acggtgcgca aaagcttgca
     8341 tctgcggtaa taagtccatc caagttaaaa acataagaag ttgtttttgc ttggctgttc
     8401 ttattacttg ttgcagagca gctttttcac aacctatttt aagcgggtga cgggaatcga
     8461 acccgcgaca tcagcttggg aagctgggat tttaccacta aactacaccc gcaatacaaa
     8521 aacagtatac gcctctttta ttccttcctc aagttttttt attctttacg cttctggtat
     8581 gttttgtagt aaaataaaac taatcaatca attattggag aagatgctat gacgggaaaa
     8641 gaattagatc tgttgctgga cacttgttta ctcgctggga aaatcatgat ggaaagcaat
     8701 gcggagatgt atcgggtaga agatacgatg agtcgaattg ctttagcttc tggaaatttc
     8761 cgtttggtca gctatgtcac tcaaaccggt ctctttattg gtcttgatcg tacctcgacc
     8821 atccgaatgg aacaaattac caaccgttcg attaatttag aaaaagtagt aaaagtaaat
     8881 gatctttcac gtaaatatgt tgcttcagag ctaacattag aagaacttta ctgcgaactg
     8941 caagcaatcg accgagaccg ttctttcttt cctgtctggt tgcaaatcgt tagcgcggca
     9001 gtgatcagtg gaacaattat ggtgttattt ggcggaaaga tgaccgattt acctgtgact
     9061 ttagtcattg gaggattggg atacgcagct tatctatata gtttgaaatt ttttagaatc
     9121 aaatttttat cggaattttt atcttcttta ttgattggca tagccgctat ctacagcgta
     9181 cgttttggct taggaagcaa tcaggatcta atcattatcg gatgtgtgat gcctttagtt
     9241 cctggagttc aaattacgaa cgctatccgt gatttgctag ctggacatta tgtctcagga
     9301 gtttctcgcg gaacagaagc gatgatgact gctacgatga tcggttttgc gattgctttt
     9361 attttccagc ttttttatta ggaaaggaac acaagtatgg ggacgatatt tattcagttt
     9421 tcttttagtt ttttagcgac tgcggcattt gccgttatta caaatgtacc taggcgttca
     9481 ttgatttgtt gtggtctttc tggtgctttt ggctggatga tctactggat ctgtgttcaa
     9541 ataggcggat ctgctgcttt tggttcattg cttggttctt taggagtagc attcatcagc
     9601 gatcttttct ctaaacgttt gaaaatgccc gtaacgattt tcaatattcc cgggatggtt
     9661 cctctagtcc ctggtggttt agcttatcaa gcggttcgaa atctcgttac tgaatcctat
     9721 cgagaagcgg ctcattatgc ggtccaagca attatgattg ctggagcaat cgctttaggt
     9781 cttgtattat cagaagtctt taaccataac atccgtaatt tcaaagtaaa gcgtgacgca
     9841 atcatgatga aacgaaaaaa acggcttttt aaataaacga agtgtaacaa agaaaaggtt
     9901 gtgaaagaga cgttcagcct tgagcgatga caaaatatct cacaaacatt gcgacaagta
     9961 accgcaggag caataagaag taattatgag tattaaaaaa agttttgtaa aaatagcttc
    10021 tcacattttt acataaaatt gacttaatac tgagaaactg acttttgaac accctctatc
    10081 tgtaaaaaag gggctgcgac atgatttttg tcacggcccc tcttctttat tcaaattttt
    10141 tccaaaacgt tcgttgataa taaacgatcc atagagcgga aagtattaga actaaaccga
    10201 tcacaggaat attcactaac caaaatggat aaaacaaagc caacattccg tataagacga
    10261 taagaataat actgatgatc aatgaaccca tcatagttgc gactaaaccc aaacttcctg
    10321 aacctctgtt aaataattgg ttaatattgg tccagttcag cagcagcaac cgataatctc
    10381 tagcgaaata acgtagtgac aataagtagc agccgaataa agaaccaaat agcaagctag
    10441 ggataaaaaa caaaggcagc tgaaaagaca gtccgcttaa taatgcgatc ccaccagtta
    10501 aaaaactctg caatatccat ccgaatctga acttttcttt taaatattgg ttcatagaga
    10561 ttggcaaaga acggataaac agaaaatttt cttgatccaa agaaataaga ttgctgataa
    10621 aagaagtctg attaactgtt aagaaagcca atgcgatccc taccaggaaa acaacaccga
    10681 ttaacttatt atctaaatca gttaatttta tttcgcctcc tagggcaaaa gcaagaataa
    10741 aaatgattgg agttaacaag gaagaagaaa gaacttgcat aatcaaatta ggttctttta
    10801 ctaattgagc attgtaacta aataaaagct gatgcacatt ttggttcttt ttatgggttc
    10861 tttttatttt gaactcactg tttgaatcag tcagttgctc tgtaagcttt ggtactatca
    10921 gctgttttac agcaaggaaa aatcccagag ccgtgatagc tagtattccc caacccaata
    10981 aaccttgttg agaaagtggc tgactggcag catcatagaa tggcaataat atcgaaatcg
    11041 gatgccgatc tgttaactca ttttcaatag tccccgtttg atgattcatc cacatgattc
    11101 cagcaaatgc gatcagcatc gagctgccaa gcaagatact agaaactgtt tttctatgtt
    11161 ttttaaacac tgctgttcga gccaaaccaa aaacaatcaa actagccaga caaaaaagaa
    11221 taatcaaaaa tagagaaaac aaaaggattg agataatgat agtaagcaaa atccctagat
    11281 cagctcgcca tccggttaaa ataaacatga ctagaagcgg aaaaacaaaa ggaatgatgg
    11341 tcaaagcaac taccaaaatc tttgctgaaa aaacctgttc ttgtccaaaa ggcagaggca
    11401 aaaatacttg caggtcattt ccttcaaaaa agatattata aatgactgat atactttgag
    11461 ttaaacctaa cataccaaaa agtgccacgt aataagtaaa gaaacctggc aatttaacaa
    11521 agttcatagc aaacatagca aatccgtaaa tcagtaaaaa aatcagccct gaaaaaagat
    11581 tttgagacaa aagatagata gttaatcgct ttcccttttt tcctttctct cttgctttat
    11641 tcgtgagctg tggattaaca tataaaagat taactcgaat taattctaat aattgtcgct
    11701 tatacatacg gtccatctcc tgtcgcttgc cttccagcca ttttcaaata aatcttttct
    11761 agcgtttcat caggtgagcc attcaataat tcactaactg ccccctgata aatcaattca
    11821 cctttcttca aaatagccag tttatcgcat aactgttgtg cggtatccaa tacatgtgta
    11881 gaaaaaatga ctgtttttcc ttttgcggca tgagccttca tcatttcttt caaatcaaat
    11941 gctgcttgag gatcaagtcc ttgcaaaggt tcatccaata cccaaatatc tggatctggc
    12001 agcaacgtgc cgatcaaaat cgttttttga cgcattccat gtgaaaaact tgcgattgtt
    12061 tcattttgat gactatacat atcaaaaagc gctgtcaatt ctgctaaacg cttttctttt
    12121 ttttgtgtgt ctaattcata ggcagctgaa atcaagtccc aatattctcc agccgtcaat
    12181 tgtaaaaaaa tatctgggga atcaggcaca taaccgattt tttgtttgat actcaatcga
    12241 ttttccgtca atttcattcc atctactaaa atcgttcccg atgatggttc aataatacta
    12301 actaaacttt taatcgtagt tgattttccg gcaccattat gtcctaaaaa tccaaaaatt
    12361 tccccttgtc ttatcgagag agacaagtct tttaatgctt gttttttccc gtaactttta
    12421 cttacattga tgaattcaat catttctgta ctccttttgt ttgttattac tttttattgt
    12481 acccttcgtt attggtggag taaatgtcaa aaagagtgca taacaaaaaa ggatagaaac
    12541 gactaggctg ctacttgggt tgttctcttc tacaggaaaa cactcttcta acaggtaaga
    12601 tatgagtctt tgttttcttt aaaatgattc attttctatc ttttcttgca taaaattctt
    12661 tagtttatca atacttctct ttcccatctc tagttgttct ccatttgcaa atgttactat
    12721 ttcgtttact ctgctaatag atgtaatgtt ttcagtatta atgatataag cttttaaatc
    12781 tttacataaa tactcatatt cgagtgattt tttatatttt ttaattgtgc cgtttacgag
    12841 aatctcttca tctttcaata ctaggcctat tttattttta taacctggca tgctcattag
    12901 ataaaggatt tcatcgtaat ggatatagtg atcgctatta ccataacgga cagttgtcca
    12961 cctttttttc gtagcatgtt gaacaggata gttttctttt tctttcaata agtaactttg
    13021 gatgcttttt ttattttttt cataagtgaa ttcttttatc aaataaccac taggcgtaat
    13081 ctttcgatta ataacagtta ttgctttatt cggatgagac gttagataaa caattttaca
    13141 cttttcattc ttttctctaa gcatttcgcc tagttcgata ccgctataat aggcgttcaa
    13201 atcgatgtct aggaaaaaaa tatctgagga atagatttca ttatttttaa tgaattcaac
    13261 aaattgtata acgttcaatc cctgaacgat ctcaaatttc caaggactta ataaaattct
    13321 ggattcttcc aaaatttcta ctagcatttt ttcaaaaacc aaactatcat ccaaaataaa
    13381 tatacgcgtt accataaaaa gtacctccac tcgatatttt actatcaaaa tcatttcata
    13441 caacttttca acatacatgt taggaataaa ttgtaattct cttcacaatc ttttccaaca
    13501 cctttgaaac cgatatcaaa tactttcagg atgctgcttc taaaataaat atcaaacaaa
    13561 tgaatacatc atgcaaaaaa agccagacaa ccgacgtcct tgcgtgtcag ctgtctgagc
    13621 tatgtttatt tattttgttc ttttaaaaag aacgatacac atactgattt ttcggagcag
    13681 agactttctc agcgtttgta acaactacag atgggatttc acgcttgaat ggttgataat
    13741 agtctgattg gatggttcct tctattttat accattcatc atttttaacc gttactcctt
    13801 ccggcatatg cgtcaacaaa ccaaagactc ctgaatctgc cacacaatgg ataatgccga
    13861 atcggaacaa gaagatatct gtattgctat cgttcggtgt ttggtaaaca aatccttcat
    13921 agctgatttt tttgccaatg aattcgctag ggtaattata aattagctcc ataacttcta
    13981 aatagttttc atctgtgatc ttgatagttt gcttatcccc atacttttct ttcaactcat
    14041 tcatttggtc taagtagtct gatttgttga aataaatact ggtatccggc tttaagtatt
    14101 gtgtctgcat ctccggatcc cctactgatt ctttacttac tgggaaatta aatcctttgg
    14161 cttctacgat cgtagtgtcg agactaactg ttggaaacaa cgtccccacg attactggca
    14221 aagctagcag tacataagcg attccacgtt gataaggttt tgacaaccca tggtcatgat
    14281 cgtgctcgtg tgccttttct tctgcttttc cgtttttgac ccacaaatac agctgaacta
    14341 aagccaaaag agcagataaa atcattgaaa gaatagctaa gtagcgataa tggacattga
    14401 tatattgatc gagtttgcca gaaacttgca agtacatcat caattcgaaa tacccagata
    14461 aaatcaaaaa ccgcagcata ttttccctcc taaataaata atgtgaatcc aataacgcta
    14521 agggcaatca gtccgatcag cgataaaata aagcgtcctg taaaatatcg tttcatcatc
    14581 agtagatttt tgatatcgac cattgggcca aagaccaaaa aggcagctac aggtgctgtg
    14641 ccgaacagac ttaacaaaga agatcctata aacgcatctg cttctgagca aagagacatc
    14701 gtaaaggcca ataacagcat gatgataatg gctaaaatct ttgttgagcc gagcgttagc
    14761 ataattctag ttggtaaata agtctgcatc gcagctgcga ttaatccgcc aatgatcaaa
    14821 taacgacctg tatcaaaaaa ttcgtcgatt gaatgtgtca acacatgccc agtttgatgg
    14881 atcagctgat tttttggttc attcttctca ctttgttctt cttcatggac atgatgatgg
    14941 tgattttctg ctactttaga cgtgattttt tctttcaata cagcgtcttt ttgaaaatag
    15001 gcaagccaga caccaatcac aagggcaaca aaaaagctgc ctatcacccg ccagcttgcc
    15061 atctgccaag aattgccaaa tgcgataaaa gtggaaaaaa tcacgattgg attgatgatc
    15121 ggagcagtca gcataaaagc aaacgctgtg tgaactggta cgcctttttt aacaaattga
    15181 tgaacgatcg gtacgatgcc gcattcacat gaaggaaaga aaaatcctaa gatcgaacca
    15241 gtaaaaatag agaagatacg attttcagga agccatctct tcacacgttc tggggtcaaa
    15301 aatacttgta aggcgcctga gataagacag cctaataata cgaaaggtaa tgcttcaata
    15361 acgatcgata agaaaatcgt actcatctgc aaaaccgaat ttggaataaa agaaaacatg
    15421 agtttcctcc tttttatttc tagttcattc gctttgcaaa tatacgcttt tattcaaaaa
    15481 aaacaagttg ctctgtaaat ttttacacaa ctcttatttt ttaccaaagc acttcaattg
    15541 actttttctg ttattcccat taaattaagg tctcatacat atcacttcta tcataaaagg
    15601 gagaataaaa atgaaacaaa ccactaactt gactgaagtt caagacatac tgcaacagtc
    15661 atccgttgcc gtcatttatc tttcgatgcc taattgcagt gtttgtcacg ctgttcgtcc
    15721 acgactagag gaacttctca cacattatga tattccggca cttcatttgg atgcatttga
    15781 aacaccagaa gtagctagta aatttgaagt gctgactgcg ccagttgtcc tgatttttca
    15841 tcaaggaaaa gaagtctttc ggcaagcacg gttcatcgac tttaaaaaaa tccgttatct
    15901 actagatgaa ctgacagcag aagatgattc cctttcttat gaagaaatct tccgaacaaa
    15961 ataatcgttg tccaaacaga aaaaagcgat tgaacaagtg aaagttttgc cttcatttgt
    16021 tcaatcgctt tttattttta ttctgctgta ttttcggaat gcgcatttgc gtcagaatgc
    16081 aagaaataga tcaaggtttg caattcattc gtcaaatcaa cattttggat caatacatct
    16141 gacggagcat tcaatcgagc agcggtaaag ttcaagattc ctttgattcc tgcttccgtt
    16201 aactgattga ctacttcttg tgctctttca gcgggtatcg ttaaaattgc tacttcgatt
    16261 tgttggatct tgatttgttc tgacatatct tccattggat aaacggggat accgtctacg
    16321 atgcgcccaa cgatttcctc attcacatca aaagcacaac tgatacgtat gctattactt
    16381 tgatggaatt tgtattttag taaagcactt cccaagttcc ctacaccgat cagcgcaaca
    16441 tttgtcaatt gatcgtcgtt taatgtcttt gcaaaaaagt gcataagatc ttctacatca
    16501 taaccatacc cacgttttcc taattcgcca aaataagaaa aatctcgacg gatcgttgca
    16561 ctatctactt gaacggcttc acttaattca gttgaagaca cttttttctt tccagcatta
    16621 tttaaaatcc ttaaatagcg ataatacaaa ggcagtcttt tagctgtcgc ctttggaata
    16681 atatgatctt tcacgaaatg acctccacgt attgtctaat tacatctttg atttctccat
    16741 catagcagta tttataggaa aaaatcaatt ccattgctca gatattgtga aattttattc
    16801 tttattcatt tatttatcat ttctgcaaat ctcgcagtat aatggaattt atggtagact
    16861 actaattgaa aaatagtttt ttatttgagg agaattcaca aagtagaaaa gaggattcca
    16921 tgatattatt acaagccaat cagattgctc gccattttgg tgcagaagtc ttatttgaaa
    16981 acatacattt agaaatcagt acaggcgctc gtgtcgcttt agttggcaga aatggcgctg
    17041 gaaaatcgac tttattaaag atcattgctg gaattgaagc acctgatgaa ggaaagatcg
    17101 ctaaaaataa aacagcgact ctcggatacc tcgctcagga cacaggactt gattccaatg
    17161 aaacagtttg gaacgagatg ctgaaagctt ttgaacaagt tagacaaatg gaagagcgga
    17221 tgcgcgagct agagattttc attagcgaag gtacgccaga cacacctgct tatagttctt
    17281 atttaaaaga atacgaccgg ctgcaacacg atttttcaga tcgaaacggc tatggttatg
    17341 aaaatgaaat tcgttccgta ttgcatgggt ttaaattcga ttcttcattt tatgatcaga
    17401 aaatcgatac tttatcaggc ggacagcgca cacgtttagc tttagctcgc atgcttttgc
    17461 aacaccctga cattttgatt ctagatgaac caacgaacca tttagatatc gagacacttt
    17521 cctggcttga gaattatctg caaagctata ctggtgcatt attgattgtt tcccatgacc
    17581 gttatttttt agataaagta gtcaatgaag tctatgaatt aagcagaaga aaaatgcgcc
    17641 attacaaagg taactattcg aaatatctag atttgaaagc agaacagtta gcaagtgaat
    17701 ggaaggctta tgaaaagcag caaacagaaa tccataaatt agaagacttc gttgctcgga
    17761 accttgttcg cgcctctacc acaaaacgag cgcaaagccg cagaaagaca ttagaaaaaa
    17821 tggaccggct ggatcgtcct caaggggatg aaaaacaagc aaactttcta ttcagtatag
    17881 aaaagccttc tggaaatgta gtcttgcaaa tcgaagatgc cgctatcgga tatgataatg
    17941 tcaccttatc tgaaccaatc aatctagata tccgtcgtca agaagcgatt gctctagtag
    18001 gtccgaatgg tatcgggaaa tcgacgttgc tgaaatcaat cattcaccag atccctttta
    18061 tccgtggaaa agaaacgcta gggacaaatg tcaccatcgg ctattacgat caaggtcaag
    18121 ctgatctgca tacaaacaag acgatcctag cagaattgtg ggatgagcat cctaccactc
    18181 cagaaaaaga gatccgaagt gctttaggtg ctttcttatt ttctggcgaa gatgtagaaa
    18241 aaccaatccc tctgctaagt ggcggtgaaa aagcacgggt tgctttagcc aagttagcga
    18301 tgaacaaaga aaattttttg attttggatg aaccaacaaa ccatttagat attgacaaca
    18361 aagaagtatt agaaaatgca ttgatcgatt atgacggcac actgcttttt gtttcccatg
    18421 accgatattt catcaatcgg atcgcgacaa aagtcgtcga actctctgaa aatggcagca
    18481 aagtctatct aggagactat gattattatt tagagaaaaa gcaggaagaa gaagaaatcg
    18541 ccgaattgtt ggcacaagat gagccagaat ctgctcctgc tcccacgaac aaatctgatt
    18601 attaccaaag caaagaacag caaaagctta tccgtagcct tcaacgaaaa attactcagg
    18661 ttgaagaaga aatggcgcgg atcgatgaat ggatcgatca gttggaacaa gaaatggtcg
    18721 cgccagaaaa cctggaagat cacgtaaaac tcaatgagct gaaccaagat ttagaagctg
    18781 ctagacaaga tcaagaaaca aaattgacgg aatgggaaga actaagcttg gaattagaag
    18841 aatatgaaaa ttaacgaaaa aaccaaaaca gaaaccattc atgctgtttt ggtttttctt
    18901 tttttatttt atcaaacaaa aattccatat ttaccttttt caccaataag aagaccaatt
    18961 actagtcatt ctcactaaag cttctaaata atagtaatca ccccaaatac aaggtgtatt
    19021 taccccatta tttttgggct tattataaac agactgtgtt aaaaagccat cttcttcttc
    19081 cgttgtagca tatctttctt tcaattgtcg cataattttc caaacagcag actggtataa
    19141 gttcttttct gttttgtcta atagacctga ttctattaat tctaataatc cacaaaccat
    19201 gatcaccata ctagatgtat ctttttcttc tctgctagaa tcatcaaaga tcaaatccca
    19261 ataacaaatg gtatcttctg gcaaccgatt cataaaataa ttggctaata ttttgcttaa
    19321 atcaaaatat ttttgttctt tcaaataacg ataacttaat gcaaaaccgt aaatccccca
    19381 tgcttggcct ctagcccaac aggaattatc actataacct tgatgcgtac ttccatatct
    19441 tggttttcca tctttgacat ccatataaaa tgtatgataa gttgaaccat cttctcttac
    19501 taaataatct gctgcatttt gaatatgagc ctgagcagct tttttatatt tttgatttcc
    19561 agtttcttca ctcgcccaat acaataatgg aagattcatg caacaatcca tgatcattct
    19621 accttgttgt gaaggatcac taggatctcc ccaagcttgt ataactcctg ccttctccca
    19681 aaaacgctga agtaatgttt ctgcagcatc aattgaagct tccttcattt ttcgatttcc
    19741 cgttatttta taatctgcta cggttgataa agaatacaaa aaaccaagat cgtgatgatt
    19801 agtgataatt tgacagtcaa tcctgtggat aaaatctata tctgcttttt cagctgtttg
    19861 ttgaaataaa ggatcacccg tcttctcaaa taacaaccaa ttaattcctg tccaaaatcc
    19921 ttctgtccaa ccaatattgt ctcttaatgg ataacgtcct tttataggac actcgcttgg
    19981 gaattgatac tgactgttac tgatacgttt gattaacctc tccgtgtgtc tgcgacaacg
    20041 ctcgatctca ttattccatt ctttttccat taattcttta taacattcat ttatccattt
    20101 ttctggatgt attatctctt caaacatcac tttatcaagc atttccccac cagcctttag
    20161 cataatttta gtcttttttt attgttttta ttttggatag ttagcccggt tgtcccatca
    20221 aaatcaattt tgggacgatt gttccatact tctaaactat aatcccgatc ttgatgagca
    20281 accactcctg ttccgagcca atgggttcct tttccaaatg taccctctaa tccaggaata
    20341 aaagtgtttt cccatgtcat caagtttgta ttgggcaaac ccataatagc tgtggatttt
    20401 cgtttaatag tatcgtcact taaacagata atacctgaaa agccagcttc actcacccta
    20461 tagttttcct tgtcttcttc tatgattatt ccttctttct caggagaaca ttcaatagca
    20521 aaccctgtct catatagaac atattctgta tctaattcta ctttgtgtac gcgaatctgc
    20581 cagccttcaa aaggaataag ccaagttctg atttttgcct gtaaagtagg ttcccatgta
    20641 gaggcaagta cctctttctt acatacaatg tctcgtacat tatttcgaac gatgaactgt
    20701 ccataggttt tgatgctctc attcttaaaa ctggtcgtga tatatgtgtc cgctcgctga
    20761 atccctagca ttgaatccat tgccatttct gctttcaatt gattcgctcg cggtatcgaa
    20821 aaaccaaatt gactagaata agcaaatttt aaatattttt cattggtcaa tgtatggaaa
    20881 tttggcccta attgtcccgc attcaaaaaa aatgtatgtc cattatcatg ttgtatgagc
    20941 aaattggctt ctgaaagaag tttagttcct tcacgaatag gcataggcat ttctttagcc
    21001 gtccaatact catggtcttc aggtagcgcc aacaataaaa aaatcttatt cagccataat
    21061 ggagataacg tactattata gggttcagtc aatgtcaatt gagggtaggt ataaccaata
    21121 ttcaggatac ctttttcatc aaatatttct ttttgcatcc accatcttat attccgattg
    21181 actaacccct tgatttcacc taatttaaaa ggcaaaagat cggcccacaa catagctgac
    21241 caaaagcttg aaactgcaaa acgataaatc atgcttcgac caaaaggcac atttgcacca
    21301 tcttccgcaa aaaaatgaat atagtctcta gaaaaatcaa tagctcggtt cttatatatc
    21361 tggctaatgg gcttttctgg ttctagtacg ctataaatca aaccataata atgaaatgca
    21421 aaaggattat aataatcttg gcgacctctt gaactgtctc gataccagcc attttgaaga
    21481 taacaatttt ctatattatc aaatgcttct ttcagtgctg attctttaac tggataacca
    21541 aatttttgga taattgttcc gacaattatc ttaaaaaatt gccaattacc atctgcacat
    21601 tcatattcaa aaatttgcga gaaccaatga ataatctggc ttttttctcc tgtatctaat
    21661 tttttccata ataattgttc atgatgatat aacgctaata acattgcggg catttctacc
    21721 atacgctggt ctctttcatg tatttctccc caataatcgg gatgggaagg atctgttcca
    21781 gcttttattc ctgatagaat taatttcaac catcgttcat ctaattggtc aattgcgggc
    21841 cccgctcccc ataaaacccg agagaatcct tccattcctg caattttatt tccatatcct
    21901 accccttgat gtatataatc aatttgtgcg ccacttgctc gataaaacgg ctggagtggt
    21961 tgatttatct ttttaaataa gtcaaagtaa tcctgtctgg tccttaatag tggtactgga
    22021 tgtttcattg tattttctcc tctttttact accaaatagc taattccgac tgttctaatc
    22081 gaagtaagcc ttcaataaaa aaataatcag aatagatcag tggcacatta taagtcagtt
    22141 ctctatggta tgcttctgct ccgcctccaa tgatcccatc ttcatccata ttccaatttg
    22201 catagtgctt ttctgtcttt tggagtatat tgatagcagc ttcatgatac atttttgatc
    22261 gtacagagga aacatatcct gctaattcca gaagaccgca tgcagcgcat actccagcac
    22321 tggtatcaat attcgaatct tttacagcaa aatcaatgag aggaatataa ccagtttttt
    22381 cgattttctg aataaaattt actgctatct tttctgctat agctagatag tacggatttt
    22441 ttgtgtaacg ataacttaac gcgaatccat ataaagccca tgcatttcct ctgctccaac
    22501 aagaattctc attgtatcct tgcccgccaa gtaaccgaat atatttccct gattctgcat
    22561 caaattctgc gatatgcccg gttgatccat ctttcctcac tagatactta gctgttgtat
    22621 ctgcatgctg ccgagcaatt ttagagaacc tcggatcacc agtttccctc gaagcccaat
    22681 acagtaatgg taaattcatc atactatcaa taatcatcca accaggttta tggtcattcc
    22741 atgctactaa atagttccct gaaatattat agcgtccagc aagtaccgta gctgcatgta
    22801 accctagttg tcgactatgt ttgttttgag actgttgata atgtgcaata gccgatggta
    22861 agaataaaaa acctacatca tgatgaacgt ctgaaaaatg gtacagggca gattctaata
    22921 attgttcttg ttgtattgct gcctctttga acttttctcg ttttgtcttg tgaaagagct
    22981 gccataaaat accactccaa aaaccattgg tccaccagtc gattcctctg tcctttatta
    23041 cgtctgtata ttttccgttg taagaaatat atggaatttt attccccatt ctgtctaatt
    23101 ccgcatctat cttttcagaa gctacattca aaatttttct tgaccattcc ttcgtctgtt
    23161 cattcatgga tatcccgcca ttcttttaat ttttatgaat gtggagttgc tcctagaacg
    23221 aactagatgg ttaacattcg ttattttttt tacttatttg cagctccaat gtcattctcc
    23281 ttaaatttag actgcgccca cacataaatc aagaccattg ggatcactga agttgctaaa
    23341 attagcataa ttgcgccata attaattggc tcacctgtac tagaaaccgt catatttttg
    23401 ataattaagg gaaccgtata tttatcttta tctgaaagca tgactaatgg aaatagatat
    23461 tgattccaac ttcccataaa gataagtaaa ccactagtaa taatcgtagg tgataaaatc
    23521 ggtacagcga ttttcgtaaa aatcacccac tcattggcac catcgattct ggcagcctcc
    23581 atcagtgctg taggaaattg actgctatat tgtcgcatca taaacactga agttgtactg
    23641 gcaatcgaag ggatgatcac acctgcataa gtattcgata atcctaaacg actcataatg
    23701 taaaataaag gaatcaatgt ggtaaaaaaa ggcaccatcc tagatagcat aactgtattg
    23761 aacaacagtt tattccccat aaaacgatac tttgctaatg cataacccgc cattgtagaa
    23821 ctggctgtag ctaaaactgt actgattaca gcaataacaa tcgaattcca aataatcaag
    23881 ggataattat atctcgccat gatggcttgc cagtttttta atagcatatc cccaaacttg
    23941 aaattgattc cgccacttaa aatttcttga ttactttgcg ttgaagctac aatcatatat
    24001 aaatatggca tcaaaaatat taaggcacca aacacaataa tgacgtacgt aaaaattctg
    24061 ctttgtatct tttgtttagt catttttatc acctccgaat ttaaattgaa tagccgataa
    24121 tataacagat acaaacacca tcgtccaggc aatagcagag gcttttccaa aatcaaccat
    24181 ttcaaaactt gttttatata aattgactcc tacgaccaaa gctgaatttt ctggtccatt
    24241 ggaagtccca aagaatatat ttgggatatt gaaattaccc aagccattga tagtggctaa
    24301 aactgtggcc attaaaatga ttggctttag catggggatc gttaataaaa agaattgccg
    24361 aaatttcgat gctccatcta tttcagcact ttcgtatatc tctttagaaa tcccttgaag
    24421 accagctaaa aataaaatag tgttatatcc tacactaagc caaatcatcg tgatgatcat
    24481 tgatattcgt atccaaaaag gatttgttaa ccactgtaca ccgttaattc ctagcgcatt
    24541 caaaaatcca ttgaataacc cagtcggatt gaaaaaagtc atgaatacgg aagctacagc
    24601 caccgttgat gtaatggttg gaatataata gacagttcta aaaaatcctt ttccttttaa
    24661 ctgtgctgaa ttcaataaaa cagcaaagta aatagctaat agcatcgtta ttggaattga
    24721 tcctgccatg ataatcaaaa cattcgtgta agatttaata aacaatggat ctcggaagat
    24781 agctagataa ttgtcaaatc caacaaattc aaatgagtag ccaattttca taaaactaaa
    24841 agcaaacgag ataatgatgg gaatcaaaat cgtcacaata aagaaaataa caaaaggtgt
    24901 gaccaatcca taagcacttt ttttatgaga cgtatctgtt gcttttttct tgctgttctt
    24961 tattgtcttg cttggtaacg aagattgcat catcgttaag tcctcctaat attttttaat
    25021 caccagttaa ttttacgccg tacttatgag caaaattttc tgcttctttt agatatgaat
    25081 tttcaaaatc acctgtttgc caatagttat atgcagcggt agtcaaataa gacgtagcat
    25141 ctgaataagt ggctggtaaa acagtatttg atgtattttc attgatttta ttcaatagtt
    25201 catagtattt ttgtcctcct tagtagtcaa actttttctc agctgcttct gtttgatagg
    25261 ccaatgagtt agatgtagcc acgcccagct ccagtgcgtt ggctaagcat tcttcatctg
    25321 tcagcataaa tgtaagaaat tgttgtgcca cttgtggatt tttggcatct ttagggacat
    25381 aaaaacttga tcctcctgat actgcacctt ttcctggatt ttcttttgaa aaaggaatca
    25441 gtggagccat gcgccatttg tcagatgcat caggaaaatt caaagacata ttcgtagcat
    25501 accagccgcc agcaacaaac attgcagatt cttgaaatgc tttctcagca tcttgtgcac
    25561 cgtagaaatg tacaatatct gagtcgatca tttctttgat taactttgca gcatgtttcg
    25621 cttctttagt attcagacgg atatttcctt cttcatcaag caaagattga ttctgctggg
    25681 ctagcatgtt gataaacaat tcttggtccc cagattctgg caaggcaatt ccaaaactac
    25741 cagttttttc ttttaatgct tttgccacct ggatatattc ttcccaagaa ctgatttctt
    25801 caaaatctac acctatttca gcaaattttt ttggttgata ataacaaagt gctggggtaa
    25861 aatcactagc ccaaggaata atcgtttcat tactcgtttg ttgacgcaaa atattcaatt
    25921 tttgatcata aaactgatcg ccatatttgt cataaaatcc atattcatca gcactataaa
    25981 aagcatcttg aaatttttcc agcacacctg ctgctgaagt gtctgccatg gagataatat
    26041 ccggcatatc ttcgtgtgtt accaattttg gggtgatcat ttgtactgtt gctgttcccg
    26101 aagctacatt cacaattttt aagtcaatat ttgctccagt tttgcggtta tagatttttg
    26161 cagcattttc aacatagttt gtcagtttgc cgtttgcgac atatacttct aatgtagtgg
    26221 ctttttttat cattggatct gcgatgtttg ctgttacttc ttcgttagtt tttccacatg
    26281 cacttaagct gaataatagg gtaatgttta gcaaagctat caccaatttg tatctttttt
    26341 tcatgtctat cccttctttc ttttcaagtt cttatataaa acgctttcac ttatgtatat
    26401 taacggaatt agtaacacaa atatttatat aaaaaaatat tttacttgtc gttttggctt
    26461 ttcatttttg ttataatagt cattaattat aaatagaaag ctttttatat aaaaagagaa
    26521 aggaaagtgg ttcttttgtt aacaaacaac caatcctcca atcttgtcaa aaaaacacta
    26581 acctattcca tagataccca tcttcattta tctgctgctc cctattttcg actactaata
    26641 aatgaaagcg ctaatttata tgctgaattt gcacataaat ctatagaact agataatcct
    26701 tcccttttat tcattgataa acagcaagaa gtccaaatct tttctactca aaaggaaaca
    26761 caaactttta tcattatcca gttttatttt gatgagcaat tattagcaca tattacggga
    26821 caatcttttt ctatagatca agaaataaca tctatcatta cttttctgaa aaatgaacag
    26881 ttcaaaattg attatctaaa acatagtaag ccagctattt cctatgatca ggaaatcaat
    26941 tcttttttca tactatgcca acagatgagc attcatttgc tttccttatt agttacttat
    27001 tgtctgcaaa aaattcaatt gtcttcctta ccaaggcatt atcaacagga acttgcttta
    27061 ttgacaatac cttcaaatga gaaagtcatt cctaaaaaaa agcatattct ttattcagat
    27121 gaattagttg atccaatcat acgatattta gaagaacatc tttcagaaaa ttttttgctt
    27181 gaacaattag cccaaatttt ttttatcagt ccagccagct taaaaaaaca ttttagaaaa
    27241 gtgaatggaa aaagtgttat tagctatttt aaagaactaa aaatggaaaa agcaaaacgt
    27301 ctcgtgctag aaaatgaatt aacctataca gaaattgcag atatattagg atttcattcg
    27361 atccatcatt tttcttcagc ttttaaaaaa tatacagggt tatccccttc aaagtattat
    27421 ctatccttaa aatagaaaaa aatttttagg ttttatccac aaattatttc tctatatttt
    27481 ctgtctattc aaaataaaca ataattttcc ggcacaatta tttgctgttt tttaccataa
    27541 catagtacga tcaacgtaga aaaggagatg tttttatgaa agttgttgtc attggcggtg
    27601 tagctggcgg tccttcattt gccactcgtt tccgtcgatt gaatgaagcg cacgaaatca
    27661 ttatctatga acgcggagaa aatatttctt atgcaagttg tgctttgcct tattatttag
    27721 gaggtgtgat cactgaccgt gactcgctga tcgaacgtac accagaaata ttgaaaacaa
    27781 aaaacaacat cgatgtcttt actaaacacg aagtaacagc aatcgatcct tctactaagc
    27841 gattaacagt taaagaccta tccacaaatg aagaaataaa aacagattac gataagttga
    27901 tcatctcttc tggtgctaga ccagattatc cggatattcc cggagttttt gaagcagaaa
    27961 acggctttgt cctccgtagt gtgacggatg cggatcgaat caaatcgttc cttgaagaaa
    28021 aaaatccaca acatgtcgtc attcttggtg caggtgttat gggtctggaa ttagctgaga
    28081 atttcaagca tcgcggctta gacgtgactt taatcgatca attgccacaa gtcgctttcc
    28141 cttatgatcc agaaattgct aatttagttt atgacaaatt gctgaaagaa ggattagccg
    28201 ttcatttaga aacaagagtt actgagatcc gtgataaagg tcgagaaata gtattatcag
    28261 atggctccgt cctttctgct gatatgctaa tttttgctgt tggtgtttct ccgaagaatg
    28321 aagtggtgaa agcaaccggc atacaattat cagatacagg tcagatcatt gttgatgacc
    28381 agttacaaac caatctgccg gacatctatg cgattggcga tattatcgaa acgactagtg
    28441 tagtgactgg tcagccgatc caaagtatgc tttccagtgc ggccaatcgt caaggacata
    28501 tgctggcaga tattttaaat ggtacgccta tgcgctatcg cggatatatt ggtgcaggtg
    28561 tcgcaaaaat ctttgatcat acagcaagtt atgcgggaat gacagaacat gaattaaaag
    28621 catcaggcat aacaaattat aaaactgttt ttatcactcc ctttgaccat gcctatttct
    28681 atccagaagc tacaagatta aatctaaagc tgatttttga tgcagatagc ggtcgtattt
    28741 taggtggaca agcatttgga gaaaaaggtg tcgataaacg gatgggagaa ctttctgtag
    28801 caatcaccgg aaacttgaca gtctttgatt tgcctgattt ggagttgcct tactctccac
    28861 cgtattctac tacccgtgac ccgttgaata tagctggtta tgtcgcaatc aatcaaatga
    28921 cgaatatcgt agaaacgatc aaagcaagtg atatacccga aaacgatttg aaagaagcgt
    28981 tctttttaga tatacgtgaa cctaataaag caccttctgg cagtatctcg gcaacaaaaa
    29041 atattccaat gaatgagtta cgagatcgaa tcaatgaaat cccaaaagat aaaaaagttt
    29101 acattacttt cagaagagga ttgaatactt atacttctgc ccgaatcttg gcaggtttgg
    29161 gtatcaaagc gattttgatt gaagaataaa aaaacgaccg cggtgggtag ccgcggtcta
    29221 ggagttattt ttacttgagg agtaaaaatg aaaaagtata tagggttgtt ggttggtatg
    29281 tatatagaat agaagataaa tgtgaagaaa ttgtgcatta tttcttcaca tttattaggt
    29341 tatagtttaa gaaagtttaa acattgcgca atttaattat tttagctgtt tcttttcata
    29401 aaaaaacatg ttgtgactct gagacaaatc atagtccata gtgatgtctc tagagtaact
    29461 acgcctaata ttacgaggca tcatattaat ctatcagcac tagtacctaa actagcatcc
    29521 ctttcgaata ttttttctta tataataaag tttgctttca ctcctctaat ctcaccaaat
    29581 cgactggctt gcgttgtgtg atactaggaa ctgattggtt tagaacgact tttctattgt
    29641 aatcatattt ttcgccatcc tcgttgtctt ctaatccttc aatgaatgat ccctaatgtg
    29701 tgtattaaat aatttaattt ttttgttcct atttatttaa
//
LOCUS       DACEUF010000029        29569 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.030, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000029 DACEUF010000000
VERSION     DACEUF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.030"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="IXG20_001245"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="IXG20_001245"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ1609453.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2486)
                     /gene="purK"
                     /locus_tag="IXG20_001246"
     CDS             complement(1353..2486)
                     /gene="purK"
                     /locus_tag="IXG20_001246"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ1609454.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="IXG20_001247"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="IXG20_001247"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ1609455.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="IXG20_001248"
     CDS             complement(3374..4681)
                     /locus_tag="IXG20_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ1609456.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="IXG20_001249"
     CDS             complement(4684..5265)
                     /locus_tag="IXG20_001249"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ1609457.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="IXG20_001250"
     CDS             complement(5837..7129)
                     /locus_tag="IXG20_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1609458.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="IXG20_001251"
     CDS             complement(7126..7749)
                     /locus_tag="IXG20_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609459.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="IXG20_001252"
     CDS             complement(7752..8588)
                     /locus_tag="IXG20_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1609460.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="IXG20_001253"
     CDS             complement(8605..9492)
                     /locus_tag="IXG20_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1609461.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="IXG20_001254"
     CDS             complement(9508..10791)
                     /locus_tag="IXG20_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1609462.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="IXG20_001255"
     CDS             complement(11474..12157)
                     /locus_tag="IXG20_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1609463.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="IXG20_001256"
     CDS             complement(12175..12792)
                     /locus_tag="IXG20_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1609464.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="IXG20_001257"
     CDS             complement(12802..13626)
                     /locus_tag="IXG20_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ1609465.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="IXG20_001258"
     CDS             complement(13619..14179)
                     /locus_tag="IXG20_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609466.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="IXG20_001259"
     CDS             14353..15309
                     /locus_tag="IXG20_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1609467.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15614..16570)
                     /locus_tag="IXG20_001260"
     CDS             complement(15614..16570)
                     /locus_tag="IXG20_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ1609468.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16674..17276
                     /locus_tag="IXG20_001261"
     CDS             16674..17276
                     /locus_tag="IXG20_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1609469.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17364..18863)
                     /locus_tag="IXG20_001262"
     CDS             complement(17364..18863)
                     /locus_tag="IXG20_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ1609470.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18879..19403)
                     /locus_tag="IXG20_001263"
     CDS             complement(18879..19403)
                     /locus_tag="IXG20_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609471.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19701..20450
                     /locus_tag="IXG20_001264"
     CDS             19701..20450
                     /locus_tag="IXG20_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ1609472.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20441..21226
                     /locus_tag="IXG20_001265"
     CDS             20441..21226
                     /locus_tag="IXG20_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1609473.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21195..21911
                     /locus_tag="IXG20_001266"
     CDS             21195..21911
                     /locus_tag="IXG20_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1609474.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22013..23032)
                     /locus_tag="IXG20_001267"
     CDS             complement(22013..23032)
                     /locus_tag="IXG20_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ1609475.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23051..24109)
                     /locus_tag="IXG20_001268"
     CDS             complement(23051..24109)
                     /locus_tag="IXG20_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ1609476.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(24330..25478)
                     /gene="nagA"
                     /locus_tag="IXG20_001269"
     CDS             complement(24330..25478)
                     /gene="nagA"
                     /locus_tag="IXG20_001269"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ1609477.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(25554..26390)
                     /locus_tag="IXG20_001270"
     CDS             complement(25554..26390)
                     /locus_tag="IXG20_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1609478.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(26574..27791)
                     /locus_tag="IXG20_001271"
     CDS             complement(26574..27791)
                     /locus_tag="IXG20_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1609479.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            28174..28257
                     /locus_tag="IXG20_001272"
     tRNA            28174..28257
                     /locus_tag="IXG20_001272"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28208..28210,aa:Leu,seq:gag)
     gene            complement(28497..29495)
                     /locus_tag="IXG20_001273"
     CDS             complement(28497..29495)
                     /locus_tag="IXG20_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609480.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         8842 a         6304 c         5063 g         9360 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaaaaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
    15361 aacaaaaata gctcctccta tttttcgttt ggctggactt attaccgcag aggaaagctt
    15421 ttgaacaccg tttaagaaag aggttgtgag cctaccgttc agctccgagt cacaaggagc
    15481 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttga
    15541 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
    15601 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
    15661 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
    15721 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
    15781 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
    15841 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
    15901 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
    15961 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
    16021 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
    16081 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
    16141 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
    16201 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
    16261 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
    16321 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
    16381 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
    16441 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
    16501 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
    16561 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
    16621 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
    16681 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
    16741 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
    16801 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
    16861 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
    16921 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
    16981 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
    17041 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
    17101 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
    17161 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
    17221 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
    17281 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
    17341 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
    17401 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
    17461 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
    17521 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
    17581 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
    17641 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
    17701 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
    17761 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
    17821 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
    17881 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
    17941 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
    18001 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
    18061 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
    18121 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
    18181 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
    18241 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
    18301 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
    18361 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
    18421 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
    18481 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
    18541 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
    18601 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
    18661 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
    18721 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
    18781 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
    18841 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
    18901 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
    18961 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
    19021 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
    19081 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
    19141 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
    19201 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
    19261 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
    19321 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
    19381 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
    19441 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
    19501 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
    19561 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
    19621 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
    19681 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
    19741 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
    19801 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
    19861 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
    19921 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
    19981 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
    20041 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
    20101 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
    20161 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
    20221 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
    20281 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
    20341 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
    20401 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
    20461 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
    20521 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
    20581 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
    20641 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
    20701 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
    20761 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
    20821 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
    20881 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
    20941 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
    21001 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
    21061 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
    21121 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
    21181 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
    21241 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
    21301 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
    21361 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
    21421 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
    21481 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
    21541 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
    21601 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
    21661 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
    21721 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
    21781 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
    21841 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
    21901 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
    21961 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
    22021 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
    22081 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
    22141 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
    22201 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
    22261 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
    22321 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
    22381 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
    22441 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
    22501 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
    22561 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
    22621 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
    22681 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
    22741 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
    22801 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
    22861 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
    22921 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
    22981 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
    23041 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
    23101 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
    23161 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
    23221 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
    23281 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
    23341 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
    23401 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga aatagccacg
    23461 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
    23521 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
    23581 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
    23641 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
    23701 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
    23761 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
    23821 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
    23881 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
    23941 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
    24001 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
    24061 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
    24121 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
    24181 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
    24241 aacttatgta aagtgatttt tactagaaca caaaaaaaac cttaaaacca aacttaagaa
    24301 gtttagtttt aaggttaact taaattatat tatgcgtgga aacgttccac gccatctaga
    24361 taagttgctt ccaatgtcat atcagggttc aatacgataa agtctgcatc gcgtccttga
    24421 gcgatttttc cacatgtatc atcgatttta cagctgattg ctggaatcag actagccatc
    24481 atgactgctt gttctggtgt tgctaatccc cagtctacaa catttttaat tgcttctttc
    24541 aatttcaata tacttccagc taagttgcca gattctagac gtgctgttcc ttcttttact
    24601 actactggaa attcgcctaa attgtaattt ccatcaggca tcccaccggc catcatacag
    24661 tctgtaatta atgcgacatg atcatgtcct gctttttcca ttaggattcc cgcagcattt
    24721 ggatgtacat gatggccatc acaaatcaat tcagaaaaga catgttctaa agataataac
    24781 gcacccacca tacctggttc gcgatgattc aatccacgca taccattgaa agcatggaca
    24841 aatacacttg cgcctgcttc aacagctttt tgcgcttctt ctagtgtagc attactgtgt
    24901 cctagagaaa caactactcc ttcttcagtc acttgtttga caaactcttc tacaccttga
    24961 cgttccggtg ctaaagcaat ttttttgatt aaaccacctg aagcttcttg ccattcatgg
    25021 aaagtatcca aatcaggatc cccgaaataa cttgggtttt gtgccccttt gtgttcttct
    25081 gtgaagaaag gaccttcaaa atagattcct ttgattttag ctcctggtgc ttcctgatac
    25141 attttgccga ttgtctcagc tacatctttt aaacgttctt tgcttgacgt caatgttgtt
    25201 ggtaaaaatg aagtcactcc acaagataac agaccttcag acatcacttt gatgccttct
    25261 gcatcattat ccattacatc atgattcata tagccgtgaa tatgtgtatc taccaaacca
    25321 ggagcaatcc atttgccgct ttggtcaatg atgttgactt ctccctcagg tagttctttg
    25381 gtgtattcac caaaaagtcc atcggtaatt tcaagatagc ctgcatgttt gacacctgac
    25441 gctaaaaaga atttgtcagc atagataaaa gttctcattc tattctctcc ctttgttttt
    25501 ctacctctaa agtactctta tttagagaag aagtcaagaa tggtctacac catttacaaa
    25561 ttttttgaaa tttgtttcac agcttgtgct aagccttcat agaatgctgc tctttctggt
    25621 tcttctttac gcttgtatat taagctttta agcacaaata aatcattgag gtaatagtag
    25681 ctattcttct cttgagccca atagattcct tgattcacct gttctaacgc ttcgtcgatt
    25741 tcattttgat ggaataagaa atttccataa ttataaaaaa tttgcgaaaa ttcacttttt
    25801 gatcgttcat cagctgattg atgcagcagt tggatgcttc gactcaaata gtagcgtgct
    25861 tcagccgttt ttcctgccac accatatatt tttccgatac aactaatcag ttgtatttct
    25921 gtgtcagaaa catggattct ttctttttgg ctagagtagc ttaatgcttc tttcaaataa
    25981 taaagggact gagagatatt ttgacgcaac gtgaattcgc aaacaccgag ataataataa
    26041 tagcgttgaa agtcagttgc ttgatacaga ttttcagaaa tatcagaaga ttttagtact
    26101 tgttctaact tccgataatc acgtttttgg aaacaactat ccaacagttc aaataattta
    26161 tcgtttgtcc gagtagtttt tcgagagaga ttcatcacat gatcaatcgt tacccctagc
    26221 cgttgacaaa tctgcatcat caccaataca ttcggcagtt cctcattatt ttcgatccga
    26281 cttaatacac tttgtgaaca aatatcatgc gaaagcattt tttgcgtcat tttttgttct
    26341 tttcgaatct ctttaatgat cgttccaaag gactcgatta acgtttccat aagtatcctc
    26401 cccaaaatat gcgtattcgc attctattcg tattttactt taggaagtcg caaacttcaa
    26461 gaaaagactt gcaactaaat aatatacttt agttatttaa gaaaactcca ctagaaagag
    26521 agcctcagtg tgtttgtctg tggccctcct actgaaaaat ttggttttta aaatcatttt
    26581 tggcgatagg ttgctaggaa ttctctcatt ttcttagctg tttgtttcaa atcatccatt
    26641 ttaggaagat aaacgattcg aaaatgatct ggctggttcc aattaaatcc accaccatgt
    26701 acaagtagaa tatgatgttc atgtaaaaag tccaaaacga atttttcatc atccaaaata
    26761 ttgaatcgct ttgtatcaat ttttgggaat atataaaaag cagcttttgg ttttacggct
    26821 gaaagaccag gaatatcatt gattgcatta tagatatatt ctctttgttc atagatcctt
    26881 ccgcctggca ggagcagctc atcgacactt tgataaccgc ctagcgcggt ttggataatt
    26941 tgctgagaca atacattgga acacaaacgc atggaggaca acatattcag cccttctata
    27001 tagcctttga cgtgtgactt atctccactt aggaccatcc agcctactcg aaatcctgct
    27061 acacgatgag atttggatag accgttcagc gtaacaacga acaaatcagg cgctaatgta
    27121 gcaatcggaa tatgttctag accatccatc actaagcgat catagatttc gtcggaataa
    27181 ataatcaggt tattttgtcg agctacttca acgatttctt ctaataattc ttttggataa
    27241 agtgccccag taggattatt aggattaatg ataacaatag cttttgtccg actcgttatt
    27301 tttgatttga tatcatcaat atcaggatac cattctgcct cttcatcgca aatataatga
    27361 acaggtgtgc ctccagctaa tgcaatagaa gccgtccaca acggatagtc cggcattgga
    27421 accagtactt catctccatt gtccagcaac ccctgcatac acatcgtgat caattcgctc
    27481 actccgttgc cggtgtagat atcattgatc gtcacattcg gaaaatgttt cagttggcaa
    27541 tattgctcaa ttgcttttct agcagaaaag atccccttgg aatctgagta gccttctgag
    27601 tcacgggcat tcatgatcaa atcacgaatg acttcgtttg gtgcttcaaa tccaaaaggt
    27661 gcgggatttc ccgtgttcaa ttttaaaata ctaatgccct cttcttgcat ccgctccgct
    27721 tcttctagaa cgggaccgcg aacatcgtaa ctgacacctt ctaatttgtt ggatttttca
    27781 aattctctca tcacttagcc aatccctcat ttcaaaaagt tagaactttc caaatttttt
    27841 gttatttact atttcaatat catactccat cctagaaaat tttgccactt atttaggaaa
    27901 aacgtaagta ttaaaaaaac acaaacaagc gttgagaact taattgtcat gatagtcatt
    27961 ccatagattc gctgcccaat ccattgcaat tccacaaatg attcctagca taaaaccaag
    28021 caaaaaacgt ccgaatgcaa gtccaatcaa tacacctaag atcattccca gtcctgaaaa
    28081 ttctccgtca tatttctcca tcttaataag tcctcccatt tttgagcttg acaactatgt
    28141 aaaacctcgc tatcattagt gtgttattat tacgcagatg tgttggaatt ggcagacaag
    28201 catgattgag ggtcatgtga gcgttagctc gtgtgggttc gagtcccatc atctgcacta
    28261 caactcgtga agttaatttt gcttcacgag tttttttgtt tgaacgtttc tttatgccat
    28321 tatatacata tcactgtatc ctttacaaaa agaacgtaca atcatgtaaa cagcctgagg
    28381 tgagtatatg aaggtcggaa acaagctact cgtagaaact tgcaagtgtt aaaacaatta
    28441 atcgaaaaac gagtaaaatc cctcacctaa tactaaagcg agggattttt tgtatattat
    28501 ctgtttatca ccgttattat aaaggtacaa ataccaagca cggttaatcc aaattgcata
    28561 aattcatttt tttgtacact catgcttttc attagctctt taactgccat tagtactaag
    28621 atggcgataa caacataaag tatatagtac gtccgcattc cggaagccca tactgtggga
    28681 gaaaatccca taattaacct tgaacaaaat cccattatca tcaaaataac taaaaaaata
    28741 gaagttttct tattgtcaaa acttaaataa attccaacaa ttaaacatag taataatgcc
    28801 aagactacta aagtagctat ccaagtacct ggataaaaaa gtgacagttt cgttcctaac
    28861 tctgtaaaga gattattcag gttactacta ctccaaatca ttgccctttt atttccattg
    28921 acgtaagtaa agctttgacc cattgtatta ccaaaataaa taatcaggtt taagaaaaac
    28981 ggtaatgcag tcaatatacg cacataatag ttttgacatt tcctatatgt tagaaggaaa
    29041 attaagaaaa atagcaataa aaataagata ttcatgtcta aaaaaaacgg ctttccaaat
    29101 gatgaaatac ctaaatctaa cttattaaga aagttgaaat ttttatattc aggaaaccat
    29161 ttattaattt catgtgcagc acgtaatgca ttccctggag ttgtcaaaga gaaaattaat
    29221 tctgctaagc ttattattga aaatactgat aacttataat tatatcgtcc attgacaatc
    29281 aaatataaac taactatact tgttagaaca aaaaaacagg cgttgacttg ttcttgatta
    29341 gttaaaaata tcaataaagg aatacttacc caatatattt ttctatttat ttcttctcct
    29401 cttaagagtt gaaaaaacgg ataaaaagtt agtaaactaa atgctactgg ccactgatag
    29461 ttcaaggtgg ttgctctcca accggtttcc cccattactg tcaatggtat taaacagtat
    29521 atagagcaaa ccaataataa atttttaaca tttattttct caaaaacat
//
LOCUS       DACEUF010000030        29110 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.031, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000030 DACEUF010000000
VERSION     DACEUF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.031"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(<1..1031)
                     /locus_tag="IXG20_001274"
     CDS             complement(<1..1031)
                     /locus_tag="IXG20_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1609481.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="IXG20_001275"
     CDS             complement(1113..1802)
                     /locus_tag="IXG20_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1609482.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IXG20_001276"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IXG20_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ1609483.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="IXG20_001277"
     CDS             complement(2329..2832)
                     /locus_tag="IXG20_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ1609484.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IXG20_001278"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IXG20_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ1609485.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IXG20_001279"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IXG20_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ1609486.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="IXG20_001280"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="IXG20_001280"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ1609487.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="IXG20_001281"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="IXG20_001281"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ1609488.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="IXG20_001282"
     CDS             complement(6444..7463)
                     /locus_tag="IXG20_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1609489.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8523)
                     /locus_tag="IXG20_001283"
     CDS             complement(7636..8523)
                     /locus_tag="IXG20_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1609490.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IXG20_001284"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IXG20_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ1609491.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IXG20_001285"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IXG20_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ1609492.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IXG20_001286"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IXG20_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1609493.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="IXG20_001287"
     CDS             9754..10398
                     /locus_tag="IXG20_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1609494.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="IXG20_001288"
     CDS             complement(10454..10957)
                     /locus_tag="IXG20_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1609495.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="IXG20_001289"
     CDS             11554..12519
                     /locus_tag="IXG20_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1609496.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="IXG20_001290"
     CDS             complement(12618..13790)
                     /locus_tag="IXG20_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1609497.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="IXG20_001291"
     CDS             complement(13777..14922)
                     /locus_tag="IXG20_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1609498.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="IXG20_001292"
     CDS             complement(14936..15868)
                     /locus_tag="IXG20_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609499.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="IXG20_001293"
     CDS             15985..17064
                     /locus_tag="IXG20_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1609500.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGISPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="IXG20_001294"
     CDS             17061..17690
                     /locus_tag="IXG20_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1609501.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17800..18027)
                     /locus_tag="IXG20_001295"
     CDS             complement(17800..18027)
                     /locus_tag="IXG20_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1609502.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18130..20718)
                     /locus_tag="IXG20_001296"
     CDS             complement(18130..20718)
                     /locus_tag="IXG20_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ1609503.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20738..21397)
                     /locus_tag="IXG20_001297"
     CDS             complement(20738..21397)
                     /locus_tag="IXG20_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1609504.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IXG20_001298"
     CDS             complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IXG20_001298"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ1609505.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22170..23036)
                     /locus_tag="IXG20_001299"
     CDS             complement(22170..23036)
                     /locus_tag="IXG20_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1609506.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23145..23786
                     /locus_tag="IXG20_001300"
     CDS             23145..23786
                     /locus_tag="IXG20_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ1609507.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IXG20_001301"
     CDS             complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IXG20_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ1609508.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25227..26126)
                     /locus_tag="IXG20_001302"
     CDS             complement(25227..26126)
                     /locus_tag="IXG20_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1609509.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26128..26925)
                     /locus_tag="IXG20_001303"
     CDS             complement(26128..26925)
                     /locus_tag="IXG20_001303"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ1609510.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26903..27607)
                     /locus_tag="IXG20_001304"
     CDS             complement(26903..27607)
                     /locus_tag="IXG20_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ1609511.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27738..28313
                     /locus_tag="IXG20_001305"
     CDS             27738..28313
                     /locus_tag="IXG20_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ1609512.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28431..29108
                     /locus_tag="IXG20_001306"
     CDS             28431..29108
                     /locus_tag="IXG20_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1609513.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8566 a         6168 c         5232 g         9144 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcatc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    17761 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    17821 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17881 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17941 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    18001 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    18061 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    18121 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    18181 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    18241 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    18301 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    18361 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    18421 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    18481 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    18541 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    18601 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    18661 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    18721 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    18781 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18841 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18901 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18961 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    19021 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    19081 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    19141 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    19201 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    19261 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    19321 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    19381 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    19441 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    19501 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    19561 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    19621 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    19681 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    19741 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    19801 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19861 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19921 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19981 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    20041 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    20101 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    20161 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    20221 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    20281 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    20341 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    20401 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    20461 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    20521 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    20581 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    20641 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    20701 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    20761 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    20821 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20881 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20941 ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca acagtttcga
    21001 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    21061 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    21121 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    21181 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    21241 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    21301 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    21361 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    21421 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    21481 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    21541 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    21601 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    21661 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    21721 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    21781 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21841 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21901 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21961 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    22021 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    22081 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    22141 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    22201 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    22261 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    22321 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    22381 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    22441 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    22501 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    22561 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    22621 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    22681 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    22741 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    22801 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22861 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22921 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22981 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    23041 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    23101 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    23161 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    23221 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    23281 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    23341 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    23401 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    23461 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    23521 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    23581 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    23641 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    23701 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    23761 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    23821 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23881 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23941 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    24001 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    24061 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    24121 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    24181 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    24241 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    24301 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    24361 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    24421 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    24481 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    24541 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    24601 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    24661 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    24721 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    24781 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24841 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24901 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24961 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    25021 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    25081 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    25141 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    25201 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    25261 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    25321 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    25381 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    25441 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    25501 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    25561 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    25621 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    25681 gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag tcgcgtcacc
    25741 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    25801 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25861 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25921 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25981 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    26041 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    26101 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    26161 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    26221 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    26281 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    26341 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    26401 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    26461 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    26521 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    26581 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    26641 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    26701 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    26761 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    26821 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26881 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26941 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    27001 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    27061 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    27121 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    27181 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    27241 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    27301 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    27361 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    27421 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    27481 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    27541 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    27601 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    27661 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    27721 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    27781 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27841 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27901 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27961 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    28021 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    28081 cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt gaagaaggac
    28141 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    28201 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    28261 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    28321 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    28381 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    28441 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    28501 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    28561 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    28621 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    28681 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    28741 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    28801 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28861 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28921 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28981 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    29041 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    29101 ctaattaaaa
//
LOCUS       DACEUF010000031        28263 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.032, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000031 DACEUF010000000
VERSION     DACEUF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.032"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(29..826)
                     /locus_tag="IXG20_001307"
     CDS             complement(29..826)
                     /locus_tag="IXG20_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ1609514.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(823..2007)
                     /locus_tag="IXG20_001308"
     CDS             complement(823..2007)
                     /locus_tag="IXG20_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1609515.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2032..3279)
                     /locus_tag="IXG20_001309"
     CDS             complement(2032..3279)
                     /locus_tag="IXG20_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ1609516.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3494..5239
                     /gene="dnaX"
                     /locus_tag="IXG20_001310"
     CDS             3494..5239
                     /gene="dnaX"
                     /locus_tag="IXG20_001310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ1609517.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5259..5573
                     /locus_tag="IXG20_001311"
     CDS             5259..5573
                     /locus_tag="IXG20_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ1609518.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5598..6194
                     /gene="recR"
                     /locus_tag="IXG20_001312"
     CDS             5598..6194
                     /gene="recR"
                     /locus_tag="IXG20_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ1609519.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6258..6902
                     /locus_tag="IXG20_001313"
     CDS             6258..6902
                     /locus_tag="IXG20_001313"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ1609520.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            6917..7246
                     /locus_tag="IXG20_001314"
     CDS             6917..7246
                     /locus_tag="IXG20_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609521.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQL"
     gene            7260..8198
                     /gene="holB"
                     /locus_tag="IXG20_001315"
     CDS             7260..8198
                     /gene="holB"
                     /locus_tag="IXG20_001315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ1609522.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8234..9058
                     /locus_tag="IXG20_001316"
     CDS             8234..9058
                     /locus_tag="IXG20_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ1609523.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9051..9398
                     /locus_tag="IXG20_001317"
     CDS             9051..9398
                     /locus_tag="IXG20_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ1609524.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9467..10339
                     /gene="rsmI"
                     /locus_tag="IXG20_001318"
     CDS             9467..10339
                     /gene="rsmI"
                     /locus_tag="IXG20_001318"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ1609525.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10448..11569
                     /gene="dinB"
                     /locus_tag="IXG20_001319"
     CDS             10448..11569
                     /gene="dinB"
                     /locus_tag="IXG20_001319"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ1609526.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11623..12225)
                     /gene="nrdG"
                     /locus_tag="IXG20_001320"
     CDS             complement(11623..12225)
                     /gene="nrdG"
                     /locus_tag="IXG20_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ1609527.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12540..14729)
                     /gene="nrdD"
                     /locus_tag="IXG20_001321"
     CDS             complement(12540..14729)
                     /gene="nrdD"
                     /locus_tag="IXG20_001321"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ1609528.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15055..15822
                     /locus_tag="IXG20_001322"
     CDS             15055..15822
                     /locus_tag="IXG20_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609529.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            15812..17854
                     /locus_tag="IXG20_001323"
     CDS             15812..17854
                     /locus_tag="IXG20_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1609530.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVINATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18135..18284
                     /locus_tag="IXG20_001324"
     CDS             18135..18284
                     /locus_tag="IXG20_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ1609531.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18295..19809
                     /gene="dltA"
                     /locus_tag="IXG20_001325"
     CDS             18295..19809
                     /gene="dltA"
                     /locus_tag="IXG20_001325"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ1609532.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19806..21011
                     /gene="dltB"
                     /locus_tag="IXG20_001326"
     CDS             19806..21011
                     /gene="dltB"
                     /locus_tag="IXG20_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ1609533.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21056..21289
                     /gene="dltC"
                     /locus_tag="IXG20_001327"
     CDS             21056..21289
                     /gene="dltC"
                     /locus_tag="IXG20_001327"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ1609534.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21292..22560
                     /gene="dltD"
                     /locus_tag="IXG20_001328"
     CDS             21292..22560
                     /gene="dltD"
                     /locus_tag="IXG20_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ1609535.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22701..23783
                     /locus_tag="IXG20_001329"
     CDS             22701..23783
                     /locus_tag="IXG20_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ1609536.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23783..24586
                     /locus_tag="IXG20_001330"
     CDS             23783..24586
                     /locus_tag="IXG20_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1609537.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24656..25396
                     /locus_tag="IXG20_001331"
     CDS             24656..25396
                     /locus_tag="IXG20_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1609538.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25407..26411
                     /locus_tag="IXG20_001332"
     CDS             25407..26411
                     /locus_tag="IXG20_001332"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ1609539.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26456..28219)
                     /locus_tag="IXG20_001333"
     CDS             complement(26456..28219)
                     /locus_tag="IXG20_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609540.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         8913 a         5002 c         5816 g         8532 t
ORIGIN      
        1 ttttttgcga acattcattt agcaattttt agaaacttct gccgccaccg ccaaaagtcc
       61 cgccaccgct agaatgggta gtacttccgc caccgccgct tccaggaggt gggcttttgg
      121 ggattcttct tgtcgtgaca aaagaatttg tcagacgatc ggttttttca gttaatttga
      181 tactggattt ttctcggaat ggatatttgt agatgcctga tttcaattga taacgcgaaa
      241 cagtcacaaa ataaaagaca agactaagga taagagccaa aacaacagca atgatgattt
      301 ctactgtcgt caaaacctta tagcgtgtaa ttttgccagt ttcttcgtct acacgataat
      361 gtcctcctgg aactccgtct tccacatatg ttgctgattt agacaggtaa gcttttgcag
      421 cattgtaata ggcagcattt gtcatctgat cataaacatc atctaagatc gaatcgattc
      481 tgctatcagt caaataatcg atcatattac cagaagtcga aatgtagatt tcacgctgtc
      541 ccatatctaa tagcaatact gagccatttt gatcatttcc gactgcgtct cttaagtaat
      601 tatcagcaaa gtctcttggt tcttctgaat tagatgtagt cgttacgatc aagactcgtc
      661 ctttgatttt ttcattcagc ttttctgcct gttgttccaa agattggatt tgatctgttg
      721 tgaataaccc agcctcatca tttaccgtag ctgtatctgc atgagtagcc accggccagc
      781 caacacttac tagcaaaaga aagaataaaa aaaatttttt catcatatca agtaacctcc
      841 gatcagaaag aggatcgcta gcccagcgaa aatcccaaga gcggtcagtc ccaatttttt
      901 ctggctgatc ggcaggacac cgctgacctt tcctgtttgt ccattcattg cgtagtagta
      961 gactttttta cttgagtcat tgcttcgata agtaacaagc cataccggca gtaatacgta
     1021 attatttact tcgtttgtga ttgaagcaga agttcgcaca ccggtcaatg ttgtatatcc
     1081 atttgcggta tcttttaaca gcttctctga atagtctttt aattcagatt gaatctgtga
     1141 tttgattgcc tcatattcga tatctctttt ttctgcttgg aacccggcta aatactggct
     1201 tttaaaatca attgctttat ctaacgggaa aggttgcacg ccttcgacca tcttttgttg
     1261 cgtatttttt gataaggcat tttttactag ttctttaaaa gaaagcttgc ctgctcttga
     1321 aacagcaaat tgcttcgttt ctgtgtattc gatgtcacct atccgccata tgcggataac
     1381 tgtaccattc gcctgcatgg caccatctac ttcagcatct gtagcccaat aagggaaata
     1441 cacacctgtc aacttatcga tttgatcctt attgaaaaaa gctttgggaa caaaccattt
     1501 tttcttggtc caagcgagaa atctgttgat tgcttcttct ttctcgacag caaaaggaag
     1561 tactttattt ggcaagaatt ctccgcttaa acgtcccgat agtacgacgg gattatggca
     1621 gtagtaacaa taagtcgcag cagttgtcgc atcagtcacg atctctgcac cacagttggg
     1681 acaaagaaaa agatctatcg ctgcggtatt atctgcagat tcgtccgctg tatctgaaaa
     1741 accgcctgcc tcgtcaaccg ctttgcgctc ggcttcattc atatcggcaa gctgttcttg
     1801 ggctgtaaaa gtaaaatctt cggtaggtga aggttcttct ttgctttctt cgccagccat
     1861 acgcgcttct ttttgctttt gctcatattg gctgacttct tcttctgtat agatattcag
     1921 gcagtattcg caatgaaatt tttggtcttt gggatcaaaa gtcaacggac cgccgcaatt
     1981 aggacattta tgtgtaagaa catccatcag ctgcacacct ttctaaataa attaattaac
     2041 ggggattcct ttaaacggtt taccacagtt tggacagaat ttagggatgc cgttggaaag
     2101 atccacgatc tcattgcatt cactgcatcg catttgtagc ttaggctgaa cgttttctac
     2161 aggtttggca gctccgcagt tggaacagaa ttttccggaa ttttctgtcc cgcaaactgg
     2221 acatgtccaa gtatccgcat ttccttgtgc agtttgttga ttttgttttt ctgcttgttg
     2281 tttgatttgt tcttggttat tttgagctgc ttgagaaaga taagaaccat ttgcattcat
     2341 tcccatacct acacccataa agccggtcat tgctcctgct tcattttttc cggctgcttc
     2401 cataccacgt gcaatggaac cttgcacgta tccttcacgt acgctaggat cacctagcat
     2461 cgccccttcg ttacgcatat tgatcagttt cacagaatca tcggtgtaag aaatgctggc
     2521 tacagccaca gaaacaatct ccatcccacg taattcacgc caagaatcat ccaatgcagt
     2581 atccatgtat ttgcttaatt ccaaactctt agatggtaca taagaaatcc gttgaccatc
     2641 tgcagacatc tgattgattg ctgattgtag cgctgttagg aattcagcta aatactgctc
     2701 attgatatca ttgatttcca cttgtgtttt gtttttaggg attgcgtttg tataaaataa
     2761 aatcgggtca acgacatgaa tagaataagt gccatgtgcc cgtaagaata attctgcgtt
     2821 atagaaattg tcaaagtagt tcaacggcgc gcttgtaccg aatttgattc cctttatttc
     2881 ttggagattg atataaaaaa cttgttgttt ctgcggtgtg acgccgccaa atttgaatcg
     2941 gtcaaaagtt tcagcaattg cttctttcag tgttccattg aacatggaag gagctgcgtc
     3001 atttttaatg gtgtaataac cttcctcagc ggtataatcg atgatttttc caccatcgac
     3061 caaaagcatc atcatgtttg gatagacatg gacgactgtt ccgtctgtta aaacatcttc
     3121 tgttccttta cgattggagc cacgtttgtc atctttgcgg actttgacac cttttgtcat
     3181 gaccgttgta tcactcatat tgtctggttc gatgacttct agccattgat ctgccagacc
     3241 gccgccaacc atacttgttg ctgctttgat aagacccata aaaaattcct cctctgattc
     3301 agctagtgcc aaacaattat tgtttattta ttataccata ggaagatgtg aaacttctcg
     3361 gactagagac ggaattgaaa tggaaaattg cgtattgttt aaatacctga aaaagccgag
     3421 aaggatagta gtttttttac ctaatatcac gtataataag agatagtgaa ttttttaaaa
     3481 aagggggatc cttatggcat atcaagcact ttatcgagtc tggcgttcgc agcgtttcga
     3541 agacatcgtt ggacaaaaag ctgtgacaca gacactaaaa aatgctattg tttcccacaa
     3601 aacctcacat gcctacttgt ttacaggacc cagaggaacc ggaaagacga gtgcagcaaa
     3661 gattttcgca aaagcaatca actgtccgaa cagtaaagac ggtgagccat gcaacgaatg
     3721 cgaaatgtgt cgatcaatta ctgctggtac gcaagaagat gtgatcgaaa tcgatgctgc
     3781 cagcaataac ggagtcgaag aaatccgatt tatccgtgat cgggcaaatt atgcgcctac
     3841 aaaggcagcg tacaaagtgt acattattga tgaggtacat atgctttcaa caggagcttt
     3901 caatgccttg ttgaaaacat tggaagagcc aaaagaaagt gtcatattca tcttagcaac
     3961 gaccgaacct cacaaaatac cagctacgat catttcacgg actcagcgat ttgattttaa
     4021 acggatcaat acggcagata ttgtcgaaca tcttgaattt atcttgaaag aatcgactat
     4081 cagctatgaa ccagctgcat tgaatgtgat tgcccgttct gcagaaggcg ggatgcggga
     4141 tgcattgagt attcttgatc aggcaatctc gtttagtgaa ggaacaatta ctttagatga
     4201 tgccatgcaa gtgacaggta gcttaactga cgaaatgatg gacagttact tgagtgcttg
     4261 cgtgaaaaaa gaagtaccag aagcattgga aacattgaac caaattctcg cagccggcaa
     4321 agaatcacgt cgctttctag aggatatgtt acaatactgc cgtgacttgc tgatgtatca
     4381 gcaagcacct aacctagttg aggaacaatc gagtacgatc agaagtacat ttacagaatt
     4441 ggcacaaact gtttcagcgg atcagctgtt ttcaatgata aagattttga gcgatactca
     4501 aaatgaaatt cgttttacca atagtgccgc tatttatttg gaagtcgcta cagtcaaact
     4561 tgcacagcta ccgtctgctt ctgttccttc agcagatgta gcatttgcag atgatcagat
     4621 agtaaaacag ctacaacaag agattaagga actaagaaac gaaatcaagg aactacgcag
     4681 taatacagga attgagaata caaaagtaca gccggtacag caaacaaatc ataaaaaaca
     4741 gacaagtacc tatcgtgtac cgacggaacg agtatataaa gttctgcggg aagcgacaag
     4801 acagcatctg aaccaagtaa aagaagtatg ggaagacttg ctgatgagtc tatctgtcac
     4861 tcagcgagca atgcttaaag caagcgaacc agttgcagct ggacctacag gactagtgat
     4921 tgcgtttgat tatgagattg tttgtcaaag agcagcaaat gacgaagaat tgcagctttc
     4981 ggtacacaac aacttaagcc gaatgatcaa agattatgcg cctgatcctg tttatattac
     5041 aagagaaagc tggccagaac ttcgaagaaa ttatttgacg caggcacaag aagagggaac
     5101 agaaaactct tttgcttctg aagactcaga aggagaaatc gagttgcttc ctcaagaaca
     5161 agaagtacca gaagaaacac ttgtagtaaa agcagaggaa ctgtttggct ccgaagctgt
     5221 tacagtggta gatgattaat aaaaaggagt agataattat gatgcgtgga atgggcaata
     5281 tgcaaggcat gatgaaacaa gtacaaaaaa tgcaaaaaga aatggcagaa gcacaagagc
     5341 tgctaaataa aacagagttt acaggtaaag ccaccaatga attggtcaaa gcggttttta
     5401 caggcgatcg tcgcatgaaa gatatccaaa tcaatgaaga agtcgttgat cctgatgata
     5461 tcgagatgct tcaggatctt gtcgtaatgg cagtcaatga tgcgatcgca caaattgatg
     5521 tggaaactga gaaaaaatta gggaaatacg caaaaggttt gccaggattt tagttttaaa
     5581 ctgaaggagt aatgaatatg caatatccag aaccaattgc gaaactgatc gacagctaca
     5641 tgaaacttcc aggaatcggt cagaaaacag cgacaagact cgccttttat acgatcgata
     5701 tgaaagatga agtagtaaat gaatttgcta aatctttgtt aagtgtaaaa cgcgatttgc
     5761 atttttgtag tatctgcgga aacatcacag aagaagatcc ctgcgaaata tgtagaaatc
     5821 cttcacgtga tcgaagcgtt atcctagtgg tcgaagagcc gaaagatgta atggcaatgg
     5881 aaaaaatgcg agaatatcac ggactttatc atgttctcca tggtgtgtta tcaccgatgg
     5941 aagggacagg tccagaagac atcaatattt cttctctgtt gcagcggctg catgatgcag
     6001 atgtaaaaga agtgattatc gcaacaaatg caacaacaga gggagaagca acagctatgt
     6061 atctctcacg tctgatcaaa cccgcaggta tcaaagtgac tcgtctagca catggtctgt
     6121 ctgtaggctc tgatatcgag tatgcagatg aagttacttt attgaaagct gtcgaaggaa
     6181 gacgtgagtt ataaatttct aattaagtgt tttttcagat acaatataaa agcagcacaa
     6241 gagatggggg caacaatgtg aacggactat ttattacaat cgaggggcct gatggtgcag
     6301 gaaaaaccag catcttgaat gaactttacc ctcttttgaa agaagtagca aaaacagaaa
     6361 tcattcaaac aagagaacca ggaggcattc cgattgctga agagatccga gcagtgatcc
     6421 ttgatccaaa aaatgatcgg atggatgaac gtacggaagc actgctttat gcagcagcaa
     6481 gaagacaaca tctagtcgaa aaggttctgc ctgctctggc aaaagggaaa atcgttttat
     6541 gcgatagatt tgtcgacagt tctcttgctt atcaaggtgc aggccgacgc atcggcgtta
     6601 ccgagattgc tcggttgaat gagtttgcta cagaaggtac tacgccggat tttacccttt
     6661 atcttgacgt agattcagac accggattaa gaagaatcaa gaaaaacaga caaaatcaaa
     6721 tcgaccgttt agattcggaa ggtttggaat tccaccagcg tgtacgtcat gcttacttaa
     6781 agctggcaga agagaatccg gaaagaatcc acaaagttga tgcaagaaaa agctttgaag
     6841 aagtgctgca aacaagttat catacaatta ttgaacagta tccgcaattt tttgaaaact
     6901 aggaaggtga acgtttatga aaattatttt agcgattatc caagacaaag acagcaaccg
     6961 tttatcaaat gaattgattg atgcaaatat ccgtgccaca aaactatcat cgacaggcgg
     7021 cttccttaag gctggaaaca gtacgtttat cgtgggtatt gaagatgacc gtgtagaaga
     7081 agcattggaa atcatcaaaa aaacgtgtga atcaagaaag caatttgtat ctacacctgt
     7141 tacactggat atttcgatgg atgggggcgt cccttatcca gtagaagtag aagtaggcgg
     7201 tgccacagtc ttcgtcttac ctgttgaagg attccatcaa ctttaggagg agctgagcta
     7261 tgaatcaaga aataaacttg gctcaaatgc aaccgatcgt gtatcagcag cttcaacgga
     7321 gttttgagca tgggcgtatc gctcatgctt atctttttga aggggagaaa ggaacaggaa
     7381 aacatgaagt aggtatatgg cttgcacagc acctgttctg tacccaaatg aaagacaatc
     7441 tgccttgtgg aaaatgcaac aattgtcagc ggatcgaaaa gaaagaacat ccagatgttt
     7501 tggttattga accggaagga caaaccatca aagtcgatca gatcagacgt ttgcagacgg
     7561 aattcagtcg cagcggttac gaatcaagaa aaaaagtttt tttgatcaaa gaagctgaaa
     7621 aaatgaatag cagtgcggca aacagtttgt tgaaattctt ggaagagccc cctggagatt
     7681 ttttagcggt gttagaaaca gatgcagtag gaagaattct ccctactatt caatctcgtt
     7741 gtcaagttct gcattttcaa gaactttcga aagaagcact gatccatcga ttgcaagaag
     7801 agcagattcc attggaaaaa gcaaaacttt tggctttctt aacaaacagt ttaggaaaag
     7861 cagttgaaat ctctcaagat gaatggttta atgatgctaa ggacttgatt tatcaatggt
     7921 tcgtttactt gcaaaagcaa gatactcaag cctttatcta tgtccaaaaa aaattggtaa
     7981 aatcatttaa agaaaaaaca caacagttta caggattatc tgtcttgatg ttttattatc
     8041 aggaagcaca gcgtcaagca gtagcaaacg gcgaatatgg caaagtgaaa cagataaacc
     8101 agataattga acggatattg atcgcagagc agaaactacg cagcaatgtc agttttcagg
     8161 ctgttgcgga acagttcgtt ttgcatacga ttttttagct tgccgcaaag gatgatgatc
     8221 aaggaggatg gaaatggtag aagtagtagg tgtccgatat agagatgcag gtcatatcta
     8281 ttattttgca ccaggagact ctgaatatgt ttatagtgac aaggtattag tcgaatctca
     8341 gcaatcctta catattgcta ctgttgtcct tcctaaaaaa gagattgatc ccaatgacct
     8401 tcctgaagct atccgtccga tcttgcataa agcgtcacaa gctgaattag aaaaagacaa
     8461 aaaaaacaaa aaagatgctt atgatgcatt gaaaatcgca aaagaaaaag tgcagttgcg
     8521 tgagttggaa atgaaaatgg tcaaagccga atacacattt gatcgaaata aattgatttt
     8581 ttctttcaca gcggacgggc gaattgactt tagagaattg gtaaaagatc tggccgctgt
     8641 atttcgtact cgtattgagc ttcgacaaat tggtgtccgc gatgaagcaa agataatagg
     8701 cggaattggt ccatgtgggc gtccattatg ctgttcttct tttttaggcg atttcgtgcc
     8761 tgtatcaatc aaaatggcaa aagatcaaag tttatctttg aatccaacaa aaatttccgg
     8821 tttatgcgga cgcttgatgt gctgtctgca atatgagagc gaagaatacg agacagcgaa
     8881 aaaagaactc cctgactttg gaaaagtaat tgctacacct gatggtaaag ggaaagtcgt
     8941 agggttgaat ctgcttagcc gtatcgttag tattcggctg actggacggg aaaatcctgt
     9001 cgaatatgaa tgggatgaat tgaaagaatt tatccaagta ggtgagatga atggataaga
     9061 gatcattata cgacggattg aatcaattag aatcagagtt gagaaataca ttaacagaac
     9121 ttgttgaaat gaagcatgct ttgcatgaga tcgtagaaaa aaatacaact ttggagatgg
     9181 aaaatcaacg tctgcgggaa cacctgcggg aattgaacca agttgcacaa gctccagctg
     9241 aaaatgtgaa gcaggaactt tctaagtctc gtatgaatct tgaaaaaatt tatgaagaag
     9301 gattccatgt ctgctacgat ttatatggat caagaaggga caatgacgag ccttgtgcgt
     9361 tttgtctaga agtgatctat cgcgaaagcg gtcgatgacc aataacaaga ggagactgga
     9421 gcggagaaat actgctccag tctttttatt aaggagattg tgacagatgt ataatcaaaa
     9481 aagtttcaag ggaaatccgg tatatgggga tttatatctt gtaccgacgc caattggaaa
     9541 tttagaagat atgagctaca gaagtgttcg tatgctgcaa gaatccgatc ttatcgcaag
     9601 tgaggatacg cgtaatacac aaaagttatt gaatcatttt gaaatccata caccccaaaa
     9661 aagtttacat gaacataatt ataaagaacg tgtccctcaa ttgattgctt tgttaaaaga
     9721 aggaaaaacg attgctcaag tcagtgacgc aggaatgcct tccatcagcg atcctggaca
     9781 tgagctagtt ttagcctgca taaaagaagg gatttctgtg atctctatcc caggacctac
     9841 agctgggatg acggctttga ttgcttcagg gctagtgcct caaccatttt tattttatgg
     9901 cttccttcca cgtaagaaaa aagaacagaa agaaacattg aaaaagctaa aagaacagac
     9961 agttacgctg attttttatg agtcacctta tcggatcact gctacattga cgaatttctt
    10021 agaaattttt ggaaatcgcc aagtcgtttt gtgtcgggaa ttgacaaagg tccatgaaga
    10081 ataccttaga ggaagtacgg aagagctact cgcttatatc gaagaacatc cagtaaaggg
    10141 tgaatgttgt ctgcttgtag aagggaattt gttcgaaatg ccagtgacgg aagttgatga
    10201 agagttggga acgttaaagg aacaagtaga agcaaagatc gcttcaggcg aaaaaccaaa
    10261 tgctgctatc aaagcagtag cgctgaaaaa tgggctgaaa aaacaagaag tctaccgtca
    10321 gtaccatgaa ttagactgag atagtataag ttgtctataa tgaaagacta atgaggaaat
    10381 cagctactaa cttttcaaat ctcttgaacg cgaacgaatg ttcttgtata atgaataaga
    10441 ggtgaggatg atggagctgc aatttccttt acataatgat acatcaagaa aaatcattca
    10501 tattgatatg gatgcttttt tcgcttcggt tgaagaacga gatcatcctg agtttcgcgg
    10561 acatccatta gtaattgcca gacaccccag tgacactggt gggcgtggag tagtgactac
    10621 agcaaattat gaagcaagaa aattcggaat tcattcagca atgagtgcac aaaaagcgta
    10681 tgagctttgt ccgcaagcca tctttaagcc tggcgaccat cataaataca gcgatatttc
    10741 caaacaagta cgggaagtat tctatcgtta tacagatatt gtggaaccag ttagtatcga
    10801 tgaagcgtat cttgatgtga ccgaaaataa aatcaattgc aagtcagcca tcaaaatcgc
    10861 ccgaatgatc caagcagata tttgggagag tgtccagcta acatgttctg cgggagtaag
    10921 ttacaataag tttttggcca aattagcatc agactatcaa aaacctagag gaatcacggt
    10981 agttgcccct caagacgctg tgccattttt aaaagctttg ccaattgaaa aatttcatgg
    11041 ggtagggaag aaaactgtac ctagaatgca tgagctaggg atttatacag gaaaagacct
    11101 atatgaatgc acagaaatga tgttgattcg aaattttgga aaaatgggct attctcttta
    11161 tcgtaaagtc agaggaatcc atgattctcc agtaaacgtt acaagagagc gaaaatctgt
    11221 gggaaaagag catacttatg ggcagccgtt gcaaacggaa gaagcagtgc tgactcagtt
    11281 gcgccaacta gctgaaaaag tagaagaagc actgagaaga gtccaaaaac atgggaaaac
    11341 agtagttttg aaggtcaggt acacggacta ttctactgtg accaaaagag tgacattgcc
    11401 agaatatatc tataaaaaag aagcactctt ttatcaagca agccttatat gggaggaaat
    11461 cttgggagta gaaaaaggaa tccgattgtt agggattact ttgacgaatc tggacccgat
    11521 gacttatgaa aacatcgtcc ttccattatg ggaaaatcaa gaaatataaa aacagctttg
    11581 agagttgaaa ccggaaatcc gttttctctc ccaaagctgt ttttatatca agtcttgctt
    11641 tttgatttgt tcataggttt ctgttgcatc cgtacatttt tcccaaatca caacttgatt
    11701 ggattctaaa gatttttgga cgtcaatgat ccgctggttg ctacttccgc gaaattgtag
    11761 ttttaggtca cgtttgctta attcaaagcg tccgtcaaca aggatatcga tttgcgaaag
    11821 gagttctaat ttatcttctg aatcttgtag caattcatca aaggtatagc ctgtccatga
    11881 ccagatatct tttgtcgtac cgaactcttt tcggatacgt cgtacgactt gcagacaaac
    11941 atctgtattc aaaaaaggtt cgccaccaag taaagttaat ccttgcacgt aatcgttgcg
    12001 caagtcagcg atgatttgtt cttctaattc aggtgtgaat ggtttgccat atcggaaatt
    12061 ttgtgcagct ttgttgaagc agccttcgca ggcaaataag cagccactaa cgtaaagact
    12121 gttgcggaca ccttcaccat cgacaaaatt aaacggtttg tagtcagcaa tatagttttg
    12181 gctgtagtct tttgctaacc attcttttgg ttttggattt ctcataaaaa aacttctttc
    12241 atttaaggtt caaagatata gctattgaag ataaaatcta ctcgaccaca taaataagaa
    12301 caaactgacc taaatccatt gttcaagaaa gcttgttctt attttgatag agggcgttca
    12361 aagatacaaa gccatcgaaa gagggtggtg aaaaaagact tgtcatagcc tcttattctg
    12421 aataaacggt gttcaaaagc ttgcatctgc agtaataagc ccaactaaac gaaaaatatg
    12481 aggaactatt tttgtttagc tattcttatt acttgatgca ggacagcttt ttcacaacct
    12541 catttcatgt gtttcacacg tgaggaaatt tctttatggc ggccatgtac catcggacga
    12601 gcttgcggat ttcctaaata gccacaagta cgtttgacta catcacatgt ttttggatca
    12661 tgatttccac attctggaca ttcgaaccct cgttctgtcg gtttgaaatc accttcgaaa
    12721 ccacattgat agcaatgatc gattggcgta ttcgtaccaa gatacccgat tttgtcatat
    12781 gcaaaatccc atacagcttc taaagctttc ggattttgtt ggagcatagg gtattcacag
    12841 tagtggatga atccacctga acaatatttt ggataatcct tctcaaattc caatttttca
    12901 aatggcgttg ggtttttacg cacatcatag tggaaactgt ttgtataata ttctttatct
    12961 gtgatatttt cgatgatgcc gaatttttct gtatccaaac ggcagaaacg gtcagttaga
    13021 ctttcacttg gagttgaata gacactgaaa tgataaccat attcatcccc ccatgcatct
    13081 gcattagctt ttaattcttt gacgatatca agtgtgaaag cttttgcttc tgggtttgtt
    13141 tcccattcgc caccgaagaa agctgaagca acttcataca atccaatgta gcctaacgat
    13201 acagtagctc gacggttttt gaataattca ttgacagaat cggtacgtga aagacgtttc
    13261 ccgaaagcac catacatata taaaattggc gcatttgctg gaatggcttc tttacaacgt
    13321 tctacacgat agagaagcgc atctttcatg atttctaagc ggtcagacag taactgccag
    13381 aatttattct gatctccttt tgattcgatc gcgattcttg gcaaattcaa tgtaacaaca
    13441 cctaaattca ttcgtccgac atttacttct tgaccatttt cgtctttcca cccttgcaag
    13501 aatgaacgac agcccatagg tactttgaaa cttccagtta attcaacgat tttatcgtaa
    13561 ttcagaatat caggatacat tcttttcgtt gcacattcca atgctagttg tttgatgtcg
    13621 taattaggat cttcagggtt taagttcaca ccttttttca aagtgaagat cagtttaggg
    13681 aagatagctg tccgcttttc actccctaat ccattgattc gaatctgtaa aattgctttt
    13741 tgaatttctc tttcaaacca gttttctcct agtccaaatc ctaatgaagt gaaaggagtt
    13801 tgtccatttg aagtaaataa ggtattgatt tcatattcca aactttgcat agcatcgaag
    13861 atatcttttt tggtttttgc ttttgcatat tctttttggc gttctggcga atcgatccat
    13921 tcttccgcgt cttttagatg ttttttatag ttcaattctg caaaaggagc aagcaattca
    13981 tctgttcggt cggcagaaca gccaccatat tgacttgacg ctacattggc aataatctgt
    14041 gagatttgtg ctgtggcggt ctggatagat tttggcgatt cgacttcggc attgccgatt
    14101 ttgaacccat tgttcagcat gcctttgaaa tcaatcaagc agcaattcgt catcggtgta
    14161 taaggatgat aatccaaatc atggtaatgg atatcgcctt tttgatgcgc atttgctata
    14221 tgtggtggca acattttcag tccgattgat tttccaacga tcccggctgt cagatctcgc
    14281 tgtgtattga aaacattact gtctttattt gcgttttcgt tcacgacagt ttggtccttg
    14341 ttgattagct tttcaatcga aaagttgata tctgttgctt tactgcgagc aaaatctcgt
    14401 ttggtacgat aattgatgta ctcttctgct aattcatatt cgtttttttc tagtaatata
    14461 tgttccacga tgttttgaat ctcatagatt ttgacatttt cagcaaaccg gcttttgatt
    14521 tcttggacaa ccaagtctgc aatcatttga attttttcat aagtaagtgg tgataatgag
    14581 ccgtgaattt tcttttctgc tttgatcagt gcttcattga tcttttgttc atcgaaatca
    14641 acatggcggc catcgcgttt gatgactttc atctgggatg tctctggctg ctgagtaacg
    14701 acagtttttt cttcataatt tactttcatt atcttcaact cctgctaaat tgttcttgtc
    14761 tatcataaaa caaaatcggg gaaggagcaa ctaaatatcg gctatatata gtattcaatg
    14821 tgctgtataa ttggatttct atactatatg tagtgtatat tttattgtga aaaaaggtga
    14881 aaacttgtag ctggtggatt tgttttttgc atatttaatt ttgctgaata aaataattga
    14941 ataaaatttt ttattcagtc caagtaaaag tgacaaaaac ataagagcaa attattcgta
    15001 gatatgctac tatgcaaata aatagaaatg aaaaatgaaa gaggaggaga agaattggaa
    15061 aagatgatcg aagtgaaaca tctaagtaaa agctacggca gcaaactttc tcccgtcaaa
    15121 gccttagata atatctcatt cacagtagaa gaaggcgagt ttgttgggat tatgggccca
    15181 agtggtgcag gaaagacaac gctgatgaat atcttgtcca cgatcaattt gcctacgatg
    15241 ggcagtgtct cgatccaagg aaaagaaatc acaaaaatga aaaacagtga gctcagtgac
    15301 tttcgtcgaa aaaaactagg attcattttt caagagttca atttgatcga taccctaaat
    15361 gcaaaagaca atatcctatt gcccctagca gttgaacgga tgactaaaga ggaaatggaa
    15421 aagagagtaa gacatgtagc tcaattgttg aatatagaag aactgctcaa acggtatccc
    15481 gatgaacttt ctgtcggaca gagacaacga attgcagctg ctagagcact tgttgttcag
    15541 ccacaagtga tttttgcaga tgaaccaact ggctctttgg actcgaagtc ggctacagaa
    15601 ttgttgaact acttgaagac catgaatcaa aaagaaaaag caacgatact gttagtcacc
    15661 catgatccat atacagcaag ctactgcgat agaattttat ttattaaaga tggtgtgatt
    15721 ttttctgaag ttgtcagaag agggacaaga agagagttct ttgagaaagt aatagacatg
    15781 caggcgacga ttggcggagg aggaaaaatg aatgctgtat aaactagccg ttaaaagttt
    15841 ttttcggcag tcacgaggct atcttgttta ttttttcagt ttgactttat ccgtaatgat
    15901 ctattattcc ttcagcgcaa tgacctacga tcaatcttta gtcagaagag caagtcaaga
    15961 tacttcgata gatggtggat tgagttttgg tggatttatc attactgttg ttttgctttt
    16021 tttcgtattc agtgccaacc gatttttctt gaatagaagg caaaaggaaa taggcatcta
    16081 tcagcttttt ggtatgaata aactgcagat atcaggtata tatgtgttag aaatcatgat
    16141 aattggattg ttcgcctgta tttctgggat attactcggt attatttttt ccaaactctt
    16201 ttcaatgatt ttggttcgaa tgatggatat ggatcttact agtccatttt ttatctccat
    16261 tccatcagta gtagatactt tatttgcttt tttcatcatc cttttagcag tatctgtata
    16321 ttcaatatgg aaaatatggc gctatccaat gattcgaagt tttggagaaa aagaacaagc
    16381 agatagttca acaatgcgct tcaggacaag gcaccgatta ctcgcagtca tcgggttact
    16441 acttttaacg agcggatatt ttggagctag tcattttagg gaaatcactt catggatgat
    16501 cagttatcat tatcttgata cattagtttt cttttttcct tttttaattt ttttcgtttg
    16561 tgtggtcggt acttatttgt ttttctgtta tacatttcgg ttatttttga tcatattgag
    16621 taaatggaag atttattact atggtatcaa ttttttgata atgggtaaca cgcaagttca
    16681 tttgctcaaa agttggcgta caaattctct gattacagtc attctcggaa tttctttaat
    16741 gacaattggc ggcacggcta gtatctctac tattttatcc taccaggaga aagtttcctc
    16801 cccgatggac tatcagtttg atgttgagac agagaaaaaa atcaagccga ttctagcaga
    16861 ggaagggcag aagataacta aagaaatcac cttgcattat aaagtcattg ggagtatcta
    16921 tagtcaaaat ctcgaagaac ctatgtttca aactgctaat ctgatcacgg aaacacaata
    16981 tcaagccttc agaaaagtta atccagattt gccgaacatt tctttgaaat cacctggtca
    17041 tactgttttg cttgatcaaa tgcaaactct ttttcggaat atttcattat atggatctag
    17101 tattggactg ccagataatc aagaattgaa gattcaacaa atgcttccaa gtgtattagg
    17161 tgacgaaaca atggtatatc taagtcctgt actgattgta gatgataccg tttttgaaaa
    17221 agcaaaaggt gtggattatc aagtagtcaa tgtagatgta tcaggtggaa ataatgaaaa
    17281 aatagatgaa cgactgagca atgacctgtc gataaagtgg ctcaatgctg tttattattc
    17341 ttatgatatc gtaaatggaa gtctacaagg agaaattagc acaaagaaac ttccggatga
    17401 ccaaatgaag gaagcacaat ttagccaaga aagctctcgt ttgaatttct cttccagcta
    17461 ttcgaaattg cgtgcagtta atcggcgaat cggtatcaat atttttgtca cactatttgt
    17521 cgggatgatc gtgattaatg ctactggtag tattttgaca gtccggcaat tagcagaagc
    17581 agaggaagaa aaagaaaatt atcacctatt gactaagctg gggattccac aaagaaggat
    17641 cagacggatg atcttcgagc aaaacgcact taccttcttt ccacccatga tattagggat
    17701 cactcacgca gtatttgcaa tcaatgtctt cactcagtat ataaaaactg ccgattactg
    17761 gttggcttat cttgtgagtg gcttattgat ccttatctat cttttgcttt atgtcattac
    17821 gagtcagtta tattgtcgga ttatcgagga atgatatacg gaataagaca agataataaa
    17881 tagaactaaa ttttgaatac catttatttg gaataagagg ttgtgacacg atttttgtca
    17941 caacctcttt attttttata ttttttaaaa gtacgagaaa ttataaagaa aaaagcagtg
    18001 atttgatgtg ctatttttat aaaagctatg tgacaaaact gttagtaaaa aaacggtttc
    18061 tgtaagctag acacatgttt ctttttgtca aaattagtta cactttgtgt gaggttatat
    18121 gaggagggaa tccaatgaac aaatggcgtt ctaatccaaa attgaattat tggggaaaat
    18181 tcatcattcg aacaattttt tatagtgcag tactgctgtt cctgatttat ctgtatcact
    18241 ataaaaatat tcaaggagga acttttatct ataacgaatt ttaggaggaa gagtatggaa
    18301 atcaaaacga ttattgaagc aatcgatgat tggggaataa atgaacctga tcgagttgct
    18361 tatcaggcgg atgagacgca tacttttggt gaattgaaac aagcatcaga cgcacttgct
    18421 tattatttag aaaaaaaggt agaaggcgat ggaccaattg tcgtttttgg caatttggag
    18481 tttgaaatga ttgtctcatt tctaggggtt gtcaaatctg gccatgccta tttgccgatt
    18541 gaagagcata cacccaagga gcgtatccta tcgatctttc gggtggctaa accatctatg
    18601 gtcatcagta tcggggactg gatagaagat attccttcgg ttcctgtcat aacaaaaaaa
    18661 gaatttcaag aaatcaagaa gataccagta ggattttatc ccagaaatag cgtaaaaggt
    18721 acagaaaatt tttatattat ttttacttct ggtactactg gagaaccaaa aggcgtacaa
    18781 atcagtcatg ataatctagt cagctttgtt caatggacga tggaagactt tgggatcaag
    18841 cctggtatgc atttcttagc ccaagcgcca ttttcttttg atttatcggt attttctatt
    18901 tatcctgcat tagtttctgg cggaatgctg aagccgttaa ataaagcagt tgttcaagac
    18961 tttcgtcaat tattcgcgac attgccagcc ttgaagttga atgtgtgggt ttctactcca
    19021 tcatttatgg atatttgctt gatggaacct acattcaatg gagaaaatgt gccggaattg
    19081 gacatgtttt tattttgcgg agaagaattg acgaaaaaaa ctgcagaaag tctacttgat
    19141 cgattcccaa atgcacggat atataatact tacggtccaa cagaagcaac tgtagctatt
    19201 tcaagtattc agattgacca gcatgtacta gatagttatg accgactacc gattggctac
    19261 gtaaaaaaag acacgaaagt ttctattgtc caagaaggta aacctgtttc aaaaggagaa
    19321 acgggtgaaa tcattattgc aggaccaagc gtatcaaaag gttacctgaa caatcccgaa
    19381 aagacagctg ctgcgttctt ttgtcaaaat gacttagcaa gttaccatac aggggatgca
    19441 ggtagactag atgaatatgg gcttttattt tatgaaggac gtatggatca gcaggtcaaa
    19501 ttacatggat accggattga actcggggat atcgaacatt atctgctcca agacaaccga
    19561 atcaaacaag ctgtcgtcgt accaaaatac caaggaacaa aagtacagca gctggttgct
    19621 tttgtcgtat tagaaaacaa aacgaaacag ccggattttc agttgactaa atctatcaaa
    19681 gaacaattac tagaagttgt aatggattat atgattcctc agaagttcca ttatgtcgat
    19741 ctgctgcctc agacagttaa tggaaaaatc gatcgaaaaa aattaacagc tgaggtgaat
    19801 cctgaatgat gttacctcat atgatccctt atgcagagcc gttttatttt tttcttttag
    19861 ccattgcgct tgtaccgatc gttctatctt tattgatatg ggaaaaaaga ttaccagttt
    19921 atcaatcagc agtgacactt ttctttttat ttgtcagctt tggaggtaat tattggcaac
    19981 agggagcggc gctgattggg tatgttttgt ggcagacgat acttgtatgg ggctattttg
    20041 tttatcggca aaggaaaaat cagacaggat ggttttttgc cgccgttatt ttagcgataa
    20101 ttccactgtt tttaacgaaa gtgactcctt tttttgaaca tggaaaagca tcgttacttg
    20161 gttttttagg aatctcttat ttaaccttta aatctgtaca aatgatcatg gaaataagag
    20221 atggcatgat caaaacatat aagccatttc gttatatcca gtttctgctg ttctttccaa
    20281 caatctcttc tggaccaatc gatcgctata ggagatttga aaaagatcag ctccatccgc
    20341 ctgttcgtga taaatatgtt gatcttttgg gaaaaggcgt ccaaaatatt tttgtcggtt
    20401 ttttatataa attcattatc ggttattatt ttggtcaagt actgcttcct gtagtgggac
    20461 gcattgcctt acagcatggt ggtatttcat gggcattagt aggttatatg tacgtgtaca
    20521 gtatgtattt gttttttgat tttgctggct atagcttatt tgcagtcggt acaagctaca
    20581 tgatgggata tcagacacca atcaatttca ataaaccttt tttatcatgg aatatcaaag
    20641 aattctggaa tcgttggcat atgacgctgt ccttttggtt ccgtgattat gtatatatgc
    20701 gattgatgtt cttcatgatg aagaaaaaat tttttaaaag caagatcgta atgtcaaata
    20761 tcggttattt tgctttgttt cttctaatgg gtatatggca tggattgacg tggtactaca
    20821 tcgtatatgg actgtatcat gcttgtctga tctgtctgac agatgcatgg atccgattta
    20881 agaagaagca taaagagcag ttgccttcta acaaatttac gcatggctta gccgtatttt
    20941 tgacatttca agcagtatgt tttagcttcc tgattttttc aggattttta gataaacttt
    21001 tttttagcta acgaatagtg acatattaat ttatataaca aaggagaaaa ggaaaatgga
    21061 agaacaaatt ttatcaatct tagaagaaat aacaggaaca gatgaagtaa gaaaaaatcg
    21121 tgatattgat ctatttgatg aagggttgct tgattcttta gcatctgttc aattattagt
    21181 cgagctagac ggagagctgg atatccaagt ccctgtatct gaattcgagc gagaggattg
    21241 gagcacaccg aataaaatca ttcaacaagc aactgcattg aagggttgag tatgaaaaag
    21301 aaactttttg ctattttcgg cccggttgtt ttagctgttg gcttactgtt tgtgtttttt
    21361 acctcctcct atcaagtaga tatctctaat ccagaaacga ttagacgtgc ctcttcttcc
    21421 atgtcagtga acgtgttgaa aggagatgcg ataaaaaatt atgcattatc tgaaaaacaa
    21481 tatattcctt tttttggctc aagtgaatta agcagaatca gtccatttca tccgtctgtt
    21541 ctggcagaaa aataccagcg aaactatcgt ccatttttgc tgggagcacc cgggacacaa
    21601 tcgttaagcc aatatatgat gatgcgttct gcaggagatg ctatgaaaaa caagaaagtc
    21661 gtctttatta tttctcctca gtggttcgtt aaaaatggtg taaaaactga ttactttaat
    21721 acgtattatt ctgaacttca aacatacgac tggttgtttt caatgaagaa agtaacgcct
    21781 gctgatcgct atttagctag aagattatta actttctcaa aagtaaaaga gaatgatacg
    21841 ttgacggcga ttctgcaaac gatcaaaaaa gggaagctac ctttacctga atctttgaat
    21901 cagctgcgca gccaatggaa catgctgaag agagaggatg aagtattcag caatattggg
    21961 ttgaaagatc gacagcaaaa aatcgatcat gagtcaaaac gattaccaaa acaataccaa
    22021 gaaacagaat tatccatttt agcaaatcaa attggagaac gagaaacgac gaataatcca
    22081 ttcgggttga aaaacgattt ttatacgcat cgtatccgag cccacgagcc agagctcaaa
    22141 cagtcacaaa aaaattggga ttatcgtttt tcgccagaat tttctgattt tcagctggtc
    22201 ttggatcaat tagcaaaaaa ccataacgaa gtattgttca tcatcccacc ggttaacgag
    22261 aaatggagcg attataccgg actatctcaa gaaatgttac aaggatttgc caaaaagatc
    22321 aaatttcaat taaatagcca aggattcaat cgaatagctg attttgtcaa tcaagcgggt
    22381 accaactatt ttatggaaga cacgatccat ctaggctgga aaggctggtt ggctgcagat
    22441 caacagatca gaccgttttt agaagaaaat catattactg ctagtaagta tcatcttgac
    22501 gatgcttttt ttagcaaatc ttggcaacat caaataccag ataaactaca attaaaataa
    22561 agaaaacagg cgagtgttgc tcgcttgttt tctttatttt tgaatattct gaataaaaga
    22621 taaaaatacc aagtttgcct gaacaaaaat cataagcaat ggtataatca caatatgaat
    22681 atagaaatgg gggaaaacat ttggatacaa aagaagaaca gctacttatt gatttaactt
    22741 ctgcaaaagg agtacctgga aatgaagaag aggtaagaga agtattcaga gaatatgcca
    22801 aaccattcgc tgatgatatt ttttatgacg gtttaggcag tgtgattgcc aaacatggtg
    22861 ctggcggagg ccccaaggtc tttatttctg gacatatgga cgaagttggt tttatggtaa
    22921 caaaaataac cgaaaaagga ttcttggaat tccaaactct tggaggttgg tgggggcaag
    22981 tcatgcttgc tcagcaagtt gaaatcaaaa cacgtgaagg gaaaatcgtc cacggtgtga
    23041 ttggttccaa accaccacat gttttaacac ctcaagtaag aaataaacct tatgaaataa
    23101 aggatatgtt cattgatatt ggcgcatcaa gccaagaaga agccaaagag tggggcatac
    23161 gtcctggaga tatggtcact ccatatattg aatacaaacg aatgaatggc agtaaatatt
    23221 tactggcaaa agcatgggat aatcgtatcg ggacagctgt ttctctgcga gtattagaga
    23281 atctatctaa agaagcacac ccaaatgttt tattcgcagg aagcgatgtg caagaagaag
    23341 taggcttgcg aggtgctcga acaagtacac accttgtaaa tcccgatatt gcttttgctt
    23401 tagatactgg tacagcagga gacactccag gaatgacacc aaaagaagca gattcgattt
    23461 taggaaaagg cccacagatt ttgatttttg atgcatctat gatcccgcat aaaaaattat
    23521 tgaattttgt tattgatgta gcagaggaaa tggatattcc attccaatat acagtgattt
    23581 ctggcggagg aactgatgca ggacaaatgc atttgacaag agatggtgtt ccgtctgtag
    23641 ccattaccgt tcctgttcgt tatctgcatt cccatacatc agtgattcat gaagatgatt
    23701 atttgaatac agtgaaacta gtcacagaat tagtgaaacg gcttgatagt gaaacagttg
    23761 aaaaaattcg gagttatgaa taatgaataa tagaatacct atttctgaat atcggaaaaa
    23821 agcacgagtt tctttggaaa atcaatgggg aatcaatact tggcttgttt tcttatctat
    23881 aatatttacg agcgtcatcc agaacttttt tgacggtttc agtccttttc cggaagaaag
    23941 catgcagcag cacttattga gttttctagt cgataattta cttttattcg cattcaacta
    24001 tgcattgttt tacattgcgc tagtagttgt acggggtggc agaccaaaaa gtaacctttt
    24061 attttcagtt ttccaaagaa agtattttgg ttcgatactt ggcatcaatt tgatcaattc
    24121 agtcatcaac tggctactca acagtttaat ctttctgccc atgtttttaa tcagcggagt
    24181 gagtacttat atgcagctgc ttttcagttc aaatgcggta cctacgggtg tcatgccaga
    24241 agattttctt gatatcagct ttgttttatc gatgataggg atgatttttg tttcagcact
    24301 catcatgagt attgttggcg gtttattcca atttgctgca tggacaaagt tcgactatcc
    24361 tgatttgtcg gttattcaat gtttgaaata tggctggttt ttattgaaag atagaattgg
    24421 tacgtatatt ttgttgcagc tgtcttttat cggctggtat ttgctaggag caatcgcttt
    24481 gttcatcggt cttttgtggg tcattgttta tgtgaatgtg tcagttgcag aattttatga
    24541 acaagcacgg attgaaaaag gaggtccaga cgcctatttt acttagcatg taagcagttt
    24601 attcatcaaa aaaataactt ttgtggtagg ataaatcttt gagggggaat gtacgatgaa
    24661 attaactgtt ttaggatgct taggagcgta tccttataaa aatcaaggga caacaggtta
    24721 tttgcttcaa tctgacgggt tcaatttgtt gatcgatgca ggtagtgcta cgctgatcaa
    24781 attacaagaa caccttgatc cacttgatct agatgctgtg atccttagcc attatcatca
    24841 tgatcatatt gctgaccttg gtgtattaca gtattactgg caacttcatc ctaagagaaa
    24901 tccggagaca ttgctttcaa tttatggaca cacaaaagat tccattcact ttgaagaatt
    24961 gacgttggaa aatgtctcaa aaggacatcc ttattttgaa gctgaagaat taaaaattgg
    25021 tccttttctg attactttta tggaaacaat ccatccagta gtatgttacg ccatgcgtat
    25081 cgtagaagaa acaacgggga aagtattcgt atttacaggt gattcaggtt atttggaaag
    25141 tttcgctgat tttgcgaaag atgctgattt gtttctagca gatacctatc tttttgaagg
    25201 aaatgaacat catcatgccc atttcacttc aaaagaagct ggagaacttg cgaaatcagc
    25261 taacgtaaag aaacttgtac ttacacacct acctcagttt ggtgatttag aacaattgaa
    25321 aaatgaagcg caaaaggcag caggcaaagg gattgatata gaacttgctg cagttgacaa
    25381 agtgtttaac atttaggagg aagaacatga tttttgtacc aaatgaaaac cacgacccaa
    25441 gaatcaattt agcaattgaa caatatttgc tgcaagaaat gcctttagat gaacctattt
    25501 tgctctttta tatcaatgaa ccttcaatca tcatcggcag aaaccaaaat acgcttgaag
    25561 aaatcaatcg agattatgtg gaagaaaatg gcattcatgt tgtccgtcgc ttaagtggag
    25621 gcggagctgt ctaccatgat tttggaaacc tgaactttag ttttatcatg cctgatgatg
    25681 gggattcatt ccgtgacttt gcgaaagtaa cacagccaat catccaagca cttcatgaat
    25741 tgggtgttgc tggggcggaa ttgaaaggcc gaaatgatct agtcattgat ggcatgaaat
    25801 tttctggcaa cgcgatgtat gctaccaacg gtcggatgtt tgctcatgga acaattatgt
    25861 tcgactctga tatcaatgaa gtagtcaatg cattaaaagt aaaaaaagac aaaatcgaat
    25921 caaaaggaat caaatcgatc cgttctcgtg taacgaatat caaaccattt cttccagaag
    25981 aaaagcaaaa aatgacaaca gaagaatttc gtcaagaaat cttgttgaaa attttcggcg
    26041 tagagtcagt agaagaagta aaaacgtacg aattgactga tgaagattgg aaaaaaatca
    26101 atcgaatctc tgaggaatat tatcgaaatt gggattggaa ttacgggaag tctccagatt
    26161 tcaatttctc tcgccaaaaa cgtttttcta tcggttcgat tgaagtccat ttgaatgtct
    26221 cagaaggaat gatcaaagaa gcaaaaatct ttggcgattt cttcggacta ggagatatct
    26281 ctgacgtgga aaaaaaattg gttggacaga aatatgataa agcatctcta tcaaaaatag
    26341 tcagccaaat tgatgtgaag aaatattttg gtgcgataga accagaagaa ttgcttacct
    26401 tactttattg aaaataaaaa atagcggaat ccacatatat ggattccgct attttttata
    26461 caggattcgg gtaatttcga gagagttgaa cagtgatccg atcaatgaaa gtatcttttt
    26521 cctgcgttag tgttcctgtg taacgatccg ctacgtcttt catcatcatt aagtcaaaac
    26581 gttcctgatc atctaattct tctgtgatgc ctcgactaaa taggtttgta tcgtaatgtg
    26641 cactatgttc gcaacaaatg acgaaatgtt cctcttgttc gtgtatcgac aaataaattt
    26701 ttcgatacag tggattttcg atggcatgag tttctctaaa ggaatgctct aaagcgtgga
    26761 tcaaaagttg agccagatcg tcatcgttga taggaagttc ttcgggaaga tctgcttgga
    26821 aggttatctg aatattccgt ttggcagcta acttctggta acgcgctaag ataagatttg
    26881 tcacctggtg gttgcaaaaa gaacacagca tttcgaaatt ttttgcttct tctaatatct
    26941 tttccacata gtttttagct tttgtttggt ccgtatgcat taaatcttgc agattctcca
    27001 tctcttggac aaattccagg cgaagtgtat ttacttcttg gatatgctgt cgaagctgct
    27061 cataatgttg ctccaacaaa tcatttttga tttttaagaa atctacttgt ctccgatctt
    27121 tgtcggtatt taagatgtga tcgattagat tgtagctgat aataacgatt agaatgataa
    27181 tatataaaat cgtagaccaa ttgatcgttg tttgatagat acctaatgcc gtttggatgt
    27241 aaatcaagca atggataatg gctaccaaga cgatccaagg tgtacaagct acataaaaac
    27301 ggttattctt atatgcttcg ccaatgcatg caagtgaagt gactaaagtg cttataacac
    27361 tagcaatatt agcaatcggc attaattcag gaagttctgc aggagtaaaa gttgcataaa
    27421 gcagaacgcc gataaaaatc aaaagttgga acgtacaaaa aattccgtac cattttttat
    27481 agtaaaacat tcggctccaa tagtagcaga tgatgaaagc tgacgttaag ataataaata
    27541 aatgagataa aaaagaatga acgaatggtt caaaaagaat accacttaaa atatcttctg
    27601 atacagcttc aaatcctaat gccaaagcaa agcaacttaa gagaataagt gagtattcaa
    27661 gacgtttttg tttatacatc aagtagatcg tatagaaaaa ggtccctata gcgataaaaa
    27721 cagcgattat catcgtcaat acttgcggca ctgattgtga ataaataaaa ctaatcaatg
    27781 gtccagttgt tcctagataa actttcggtg gcagtccagc atagttctga taaggagatg
    27841 agactgtaag ggtcaatttt tttccgatat aatttgctgg taaatctatc acagccaatg
    27901 agagtccggg attgctttct tcctgataat ctttccgttg gtataaaatc ttttcgccta
    27961 gatatacagt aatccattga tggtttcctt tgattagtaa atttgcttcg tccaacggtt
    28021 tttcaagtgt cgtttccata atgaatgttt cgtctttatc cacggtagga atcgtacgag
    28081 tgtacttgct gtcaaactta aaatcaggat catcttttgt atagaaaacc cacgaatctt
    28141 ttatttcttt catttgattg ctgtccgcag gagaaaaaat aaaataaaag aaataagcaa
    28201 ttcctgttaa aaataacaat gataatataa tgattatgct ccactttata cgtaaataaa
    28261 aag
//
LOCUS       DACEUF010000032        27966 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.033, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000032 DACEUF010000000
VERSION     DACEUF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.033"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(477..2369)
                     /locus_tag="IXG20_001334"
     CDS             complement(477..2369)
                     /locus_tag="IXG20_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609541.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2366..4093)
                     /locus_tag="IXG20_001335"
     CDS             complement(2366..4093)
                     /locus_tag="IXG20_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609542.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(4109..4759)
                     /locus_tag="IXG20_001336"
     CDS             complement(4109..4759)
                     /locus_tag="IXG20_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1609543.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4963..5259
                     /locus_tag="IXG20_001337"
     CDS             4963..5259
                     /locus_tag="IXG20_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1609544.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5451..6071
                     /locus_tag="IXG20_001338"
     CDS             5451..6071
                     /locus_tag="IXG20_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ1609545.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6163..7329)
                     /locus_tag="IXG20_001339"
     CDS             complement(6163..7329)
                     /locus_tag="IXG20_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1609546.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7341..7694)
                     /locus_tag="IXG20_001340"
     CDS             complement(7341..7694)
                     /locus_tag="IXG20_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1609547.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7822..8844
                     /locus_tag="IXG20_001341"
     CDS             7822..8844
                     /locus_tag="IXG20_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ1609548.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9283..9717)
                     /locus_tag="IXG20_001342"
     CDS             complement(9283..9717)
                     /locus_tag="IXG20_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609549.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9827..11686)
                     /locus_tag="IXG20_001343"
     CDS             complement(9827..11686)
                     /locus_tag="IXG20_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1609550.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11804..12964)
                     /locus_tag="IXG20_001344"
     CDS             complement(11804..12964)
                     /locus_tag="IXG20_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ1609551.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13271..14695)
                     /gene="araA"
                     /locus_tag="IXG20_001345"
     CDS             complement(13271..14695)
                     /gene="araA"
                     /locus_tag="IXG20_001345"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ1609552.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14711..15409)
                     /locus_tag="IXG20_001346"
     CDS             complement(14711..15409)
                     /locus_tag="IXG20_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1609553.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15366..17027)
                     /locus_tag="IXG20_001347"
     CDS             complement(15366..17027)
                     /locus_tag="IXG20_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ1609554.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17245..18645
                     /locus_tag="IXG20_001348"
     CDS             17245..18645
                     /locus_tag="IXG20_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ1609555.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18897..19976)
                     /locus_tag="IXG20_001349"
     CDS             complement(18897..19976)
                     /locus_tag="IXG20_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1609556.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(20062..21021)
                     /locus_tag="IXG20_001350"
     CDS             complement(20062..21021)
                     /locus_tag="IXG20_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ1609557.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(21033..22556)
                     /locus_tag="IXG20_001351"
     CDS             complement(21033..22556)
                     /locus_tag="IXG20_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1609558.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22566..23129)
                     /locus_tag="IXG20_001352"
     CDS             complement(22566..23129)
                     /locus_tag="IXG20_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1609559.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(23135..23998)
                     /locus_tag="IXG20_001353"
     CDS             complement(23135..23998)
                     /locus_tag="IXG20_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1609560.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(24012..24908)
                     /locus_tag="IXG20_001354"
     CDS             complement(24012..24908)
                     /locus_tag="IXG20_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1609561.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(25083..26354)
                     /locus_tag="IXG20_001355"
     CDS             complement(25083..26354)
                     /locus_tag="IXG20_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1609562.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26344..27861)
                     /locus_tag="IXG20_001356"
     CDS             complement(26344..27861)
                     /locus_tag="IXG20_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1609563.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8169 a         5883 c         4852 g         9062 t
ORIGIN      
        1 gggcttatta ccgcagatgc aagcttttga acaccgttta aggaagaggt tgtgaaaaag
       61 tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttctaca tttttgcata
      121 aatttggctt aataccgaga aactggcttt tgaacaccgt ttaccaagag gttgtgagcc
      181 tgccgtttaa ctccgagtca caaggagcaa tttcaaaaaa cagcttctca ggtttgttga
      241 ctattgtgac ttgtgaccga ggagttggca ggagaacacc gtttaaggaa gaggttgtaa
      301 aatcagcggt ttgacctgag tattagagat acaaaacgga aaaatagctt ctcatatttt
      361 gccgtttttt gagctaatac cgaaggacag ctgattgaac accgtttatt cggaaaaagg
      421 gactgtgaca cgacttttgt cacagcccct aaaaaaaata ataaatttgt actattctat
      481 gaagcttctt cttcagaaaa ctgactgtta taaagatctg catagaatcc gtctttttcc
      541 atcaatgtat cgtggtttcc tgtttctacg atcgaaccat gattcatcac aataatgttg
      601 tctgcatcgc gtattgtaga caagcggtga gcaacaacga aacttgtccg gttttccaat
      661 aggcggttca tcgctttttg aatcagtatt tctgttcgtg tatctacact agatgttgct
      721 tcatcaagga tcaggacatc tggatttgct aagaatgccc gcgcaattgt gattagctga
      781 cgctgccctt gcgaaatatt gcttgcatcc tcgtttaata cagtgttata tccttctggc
      841 aatttacgaa caaaatcgtc tacgtgagca gctttagcag cctcaatgat ttgttcgtct
      901 gttgcatcgt cgttcccata tttgatattg tcatagatac ttcctgtaaa cagccaagta
      961 tcttgcagta ccattgaaaa ctgtgcgcgc aattcatcac gtgaaagatc acgggtatct
     1021 acaccatcgt aacgaatact acctccagaa acatcgtaga accgttcaag aaggttgatc
     1081 agtgtcgtct tccctgcacc agtcggacct acaatagcga ccatctcacc aggtttgaca
     1141 tccaagttaa aatcagtcat tagcaagtta tctggtgtgt agccgaattg aacattttca
     1201 aaacgaactt tgtatggtgt atctttttct tctggaaggc cagatttttc attggacatc
     1261 tcttcttcat ccaacacttc aaatacccgt tcggctgaag caactgttga ttggatagtg
     1321 ttcatcaaac ttgcaagttg agtgattggt tgagagaact gtgttgtata ttgcaagaac
     1381 gcttgaacat cccctaatgt catgttccca tttgcaactt ttactccgcc tagtacagcg
     1441 acgaacacat agcctagatt tttaacgaaa ttcatcaacg gcatgatgat tgccgaaata
     1501 aattgagctt tccaaccagc ggcatacaga ttctgatttt ctttttcaaa tacttcctgg
     1561 tctttttcag tatgattgaa tgttttaaca atggtatgac caccatatgt ttcttctact
     1621 tggttattca ataaacctaa acttttttgc tgatctgcga aaaacacttg tgatttcggc
     1681 gctacgatca tcacgatgat caagcttaaa gggatcatca ataacgcgac taacgtcaat
     1741 tgccagctga ttgaaagcat catcacgatg accccaatca atgtgactgc actagttaca
     1801 aattgagtca aattttgctg cagcgtgctt gcgatgttat ccatatcatt gattgctcga
     1861 gacatgatat ccccatttgt atgtgtatca taataggaaa tcggtacacg attcattttg
     1921 gcttccaaat catgacgtaa ttcataaact gttcgttggg aaacacgtgt cataataaat
     1981 tgctgtaaaa agttgaatac agcagaaatc aaatacatca cgattactgt cgcaatgatc
     2041 tgcgcgattt tatcaaagtc tatcggaaat tttgttactt gttggccttg ttgcatctgt
     2101 tgagcgccag ccattacacc tttgaatatt tctgtggttg ctttccccaa gacctttggt
     2161 gtttggattt ggaagatcac ggctgctatt gctagtacaa atacagcgat gatcgcaacc
     2221 atacgtttag acatatagcc aaacaatcgg cgtaccgttc cccagaaatt tttaggcttt
     2281 tcacctttta caccgagatt tctgccaggt ccaccatgtc ctggaccgcc tgctggtcca
     2341 tgagagggac gtgtgtttgt tttactcatg ctaagtcctc ctctcttagt tgtgaatgaa
     2401 cgatttcttg ataagtttcg tttgttgcta atagttcttc atgtgttcct ttgccaacca
     2461 gttttccttc atctagcaca aggatcatat ctgcatccat tactgtactt actcgttgag
     2521 caactaaaac ggtgatactt tctttcatat tcgttttcaa tgcttggcga agattggcat
     2581 ccgttttgaa atccaacgct gagaaggaat catcaaacac atagatatct gctgttttga
     2641 ctaatgcccg tgcaattgcc agacgctgtc tttgtccacc agagaagttt cctcctcctt
     2701 gttccacatg gctatctaag ccatcttcca tctctgaaac aaaatcttta gcttgtgcga
     2761 tttctaatgc tttccaaatc atttcatctg ttgcatccgg cttaccatac tgcatattgt
     2821 ctcggatcgt tccagagaac agaactgctt tttgaggaac aaagcccatt aattctcgca
     2881 aattatattg agtcatgtca ctaatgtccg ttccatttat ttggatcgaa ccagattcga
     2941 tgtcatataa tcgagggatc aaattgacta aggtcgtttt acctgaacca gtccccccta
     3001 tgattgcgac gatctcgccc gacttggctt ggaaatcaat atcttccaat gcaagttttt
     3061 ctgctccatg atatcgataa ttgacatgat tgaaggccaa tgttgcgttt tttccagcaa
     3121 aagaaactgt tttaggattt tcagggtcat ggataccgat tttttcatcc aacacttcat
     3181 taatacggtc agctgaagct tgtgcacgag gcaccatgat aaaaatagcg gaaagcatca
     3241 taaagctgat caagatctgc atcgcataag tcataaatgc aatcagatta ccgacttcca
     3301 aagtcatatc tgctatgtag tgtccaccaa accaagtgat tgctacgttt gtaccgctca
     3361 tgatcaatgt catcattggc atcatcaatg cgacgatcgt attaactttg attgccgtat
     3421 ttgtataatc tttattcgct aaatcaaaac gattttcttc aaagcgtgtc ttatcgaatg
     3481 ctcgaatcac tctgacccct gtcaatcctt ctcgaaatac caggtttaaa cggtcagttt
     3541 tcttttgcat acttttgaat aatggaacag caaagtacat gattccgccg atcaaaataa
     3601 tcattactgg gatgacaact aagaagattt ttgtcaactg atgatctttt tgataagcaa
     3661 gtatgctcgc gccaatcaac gtgattggtg cattgatcat catacgtaaa aacatttgtg
     3721 taaccatttg gatttgagtc acatcattgg tcgttcttgt gatcaaagag gccgttccat
     3781 atttatcaaa cgcatctttt gtcagactct ctgatttttt atagacatcc gtacgcaatt
     3841 gcttacccag tttctgtgat tcacgagtag caaaaaatgt atttgcgata gcagctaaga
     3901 tactgatcaa agaaaagccg atcatcacaa aacctgtatg ccaaatataa tcgacatctc
     3961 cttgcgctac ccctttatca ataatattgg aggtaagtgt cggcaaatat aaatctgcca
     4021 gtacttggat caccatgaaa actgcagctg caattgctga agtcaaggag atccgtttta
     4081 cgatttttag catctagttc ccttctttct atttatatac tccgtttttc agccaagaaa
     4141 gtttcaaatt gaattcactg attgcttgct ctactgagta atcttcagat gttgaaaata
     4201 agcgatacga atgcgcaatt gttgtaaata cagtattcat cactaattgg atcagtaatt
     4261 ccaattcatg atcatttttt actttcagct tcgtccgatc gatcaattca agcaactctt
     4321 ttgcatcttg tttgtgttct ttatgtggat cgtgaggcgt tttttggcga ctcgtatgag
     4381 gagcgacttt tcttgaggca cggtaatcca tgttcataaa caagttacga taaaaagaag
     4441 cattctcgcc ttccaaaatc tcataaatca ttttagaaaa ataggattcg aatcctttga
     4501 atagatcacc atcggattct ttcattagtt gtaacatatc tttagagctg tcacgtctca
     4561 ttgtctcaaa gtaataaaaa taaagatcct ctttatcttc aaaatactga tagaaactgc
     4621 ctctagggat atccgctaat ttgacgatat tagcaatcga agcttctttt aagggtactc
     4681 tagaaaactc gattctcgca gcctcgatta gtcttttttg tttttcttta ggcaaatgga
     4741 aaaatgtctg tgtcggcata tggattctcc tttctttcaa gatgacacat tgtcatatga
     4801 cgctatgtca ttataacacc tcctacctgt tttgcaagac atttgcatag tttttttcat
     4861 tatcatccga tacattaaaa aagtgataag aggaatcctt ccatttgaat taatgagagt
     4921 tgcccattaa aatagccaca tactaaacaa aggatgagat aaatggccca atcaattagt
     4981 gtccaagact tcaataaatt gcctctcgat gctgacagca tcgttttaga tacaagaaat
     5041 accttagatt acacaaaaaa ccacttaagc cactcgcttt ctatccccgc tgctatcttg
     5101 ccaagacaat taaaaaatct agatccaaag aaactttatt atattctaag ctatacaggc
     5161 aggcgatcag aagcattagc cgatcaactt actgctaatg gttttcgtgc tgtttatatc
     5221 attggtggga tggaagctct tcagtcgagt gttgcttgaa tattgcttgt tagattcaaa
     5281 agaggctgtg acaaaaaatc gtgtcacagc ctcttttata ccctgtcttt ttttagttat
     5341 ttattcttca accttatcaa tatgacttga tagccttttc tttttcgagt aaactgattt
     5401 tgttagtcat agttataaca atcatagcaa taaaaggaga aggtaagcat atggcatctg
     5461 ttttagtagt caaaggtcat cccctaactg cagaagaatc gcgcacagtt aaggcattga
     5521 cgtctttttt aacaagttat aaagaaaatc atccagatga tgaagtgact gttttggaac
     5581 tttatcgcga tgatattccg gaaatcgatg aagaattatt atcaggttgg gaagcattac
     5641 gtgctggtgc agaattcact tccttatcag aaagtcaaca acaaaaaatt gctcgtttca
     5701 atgaattgac tgatcagttc cttgctgctg ataaaatcgt tatcgcaaac gccttatgga
     5761 atctaaatat cccaacaaaa ttaaaagctt ggttcgatac cgtcaatgta gctggaaaaa
     5821 cgttccgcta tacagaaaac gggccagaag gattagtgac aggtaaaaaa gcactgcaca
     5881 tccaatctaa tggcggtgta tacaacggac aagactttgc ctctcaatat gtaaaaggta
     5941 ttttaaactt tgtaggcatc gatcaagtag atcagctatt catcgaagga atcgattatg
     6001 accctgaccg tgcagatgag ttgatgcaaa atgcattaga taaagctgct gctttaggta
     6061 aatcatttta aaaattgaga taaatgcata caaaagagac agaggaaaaa atcttcaaag
     6121 cagcatttgt gccttggata ttttcccatc tgtctctttt tgttattctt tctgcttttt
     6181 caacggattt tcttcataaa gagaagagat tgttgtgtac cattcaaaaa catgagtaat
     6241 cactacttta gctgctgcat aaacaggaat ccccaagatc acaccgatga ttccgaacaa
     6301 tttcccagaa gtcagcagaa ctaataaaat cgttactgga tggattgcta actgattccc
     6361 tagtactaat ggagagatca agcgtccttc tattgtctgc tctactgcaa aaacgatcag
     6421 tactttgatg atcatgaatg gacctgctac aatggctaaa aagatagccg gaatcatcgc
     6481 taaaaatgat cctaagtaag ggatcaggtt taaaaaaccc gcaataatcc ccaacgtgac
     6541 agcatactct aaaccaatga ccgcaaaacc aatcataaac atgattgcca cagcaaaagc
     6601 cacagttagt tgtcctctta tataagaaga aacttgacta ttcatttcac tcagcacatt
     6661 caacgtcggt ttttgcatgc gtgtcggtaa aaatttgaca aaataaggtg ctaattgttt
     6721 accgtctttc aatagataaa ataagataaa cggcatcgtc agcagagcca ctaaaatagt
     6781 cgctaccgcg ccgacaaaac ttccaagact ctgaactgta ctcttagata tatcttggat
     6841 catatcgcct gccgaattca tgaacttatc acctgctgtt tccaactgtt cgcgaaattg
     6901 gttgaacagc gggtcccgca aaatctcggt taatttatta tcaatcgtat ctacatattg
     6961 agggaagtta ctgatgaagc tcactgtttg ttcctgaatc ttagggatga tcactacgct
     7021 cccccatgcg attagtgcaa caaccaaaac aaacagccca atgatactgt aaagccttgg
     7081 tacttttcgc ttttcaaagt aatcaactac cggattcatc aagtaataca agattcctgc
     7141 taaaatgata ggcagaccta cgattgctaa aaattgccag attggtgcaa acaaatatga
     7201 aacttttgta aataaaaaga cgatcagcaa tattaacaat acaacaagta aggctgtgac
     7261 tacttgatta tttaaaaacc agcgccaaaa ccacgatacc ggtctgttct tcttattttt
     7321 tccttccata attcctcctg ttagttataa aaaatttggc ttcccaaacc aatagcaggc
     7381 agtctatgcg gatgaagata gattccattt gcaatttgtc cttcttctgg acaagcatca
     7441 aaaatcaatg atacgtgagc tactgtcttt aaattcccat tggccatcgg accgacataa
     7501 tcgatcgtat atacctgatc atcaaaagca attgttccgc ctgctttcaa agaaaaaggt
     7561 tcgtcttttg atacttcttg aatcaccgaa tattttcgta aggcagctgt tgctgtttta
     7621 tcgaacaaaa tcagcatggg ttcttccttg tttattgctt ctgtcccaat ttctgtaatc
     7681 tttgcctgaa tcattgcact ctctccttta attgctttca aaaacagtat aacacattct
     7741 cattctttag ccgtgtggaa taattatgtg atagaataaa aacgaaaact atttcattac
     7801 aattaggagg aatcagctca tatgatcgaa aagataattt taggaaccta tacgcgtcgc
     7861 gaaagtaaag gaatctatac gatcgacctt gatacagaaa aagaagaatt agccaacctt
     7921 tcattagctg ttgaagaaaa tagccctacc tatgtagcaa aaagtaaagc tggcaatctt
     7981 tacacagtta ctagtgtgga tggattaggt ggtgccggtg cgtacgataa ggattttcat
     8041 tttttgaatg cggtaacaga gtctggcgct cctttgtgtt atgtagcggt tgatgaagca
     8101 cgtcaattac tatacggggc gaactatcat aaaggtgaag taaatgtcta ccatatatta
     8161 ggagatggcg aactgaaagc agcagattca atattccatc aagaagcgac aggtcctcat
     8221 aaaaaccaag atcatgcgca tgtccactat acggatctga ctcctgacca acgattagtc
     8281 gtatgtgatc ttggtacaga tcgtgtgtat acttatgatg tgtccgaaaa cggcaaattg
     8341 aatcttgtaa ctaccttcac tgcagagcca ggaacaggcc ctcgccatct tgtctttcat
     8401 ccaaatggac aattcgctta tttatttggt gaattagaca gcacagtccg cgtgctttct
     8461 tataataaag aaacaggtgc ttttgaagaa ttacagaaaa tatcgacctt gccagaagaa
     8521 ttcgacggtg aaaatggagg ggcagctatc cgcatttcaa atgatggcaa gttcctttat
     8581 gcttctaacc gaggacataa ctcgattgct gtattcgcag tttttgaaga tggttcccac
     8641 attcaaaaca agcaaatcat ctcaacagaa ggggattttc cccgcgattt cgcactagat
     8701 ccaagcaata attttgtcgt gtgtgccaat cagaacaccg ataatctgac attatatcgc
     8761 cgtcatcctg aaacaggatt actggaatta ctgaaaaaag atgtctatgt accagaatgt
     8821 gtctgtgttt attttgatcg ttagtaagaa aaggagaatg aaatgaatca gcaagacgta
     8881 ttagacagac tacgggaaga actaaatatt cctttctttg atgggaaaat cgaagataaa
     8941 gagtatagcg aagaggaata tcagaaactc aaagccgatt tacagaacta ctttgaacaa
     9001 tatgtacaga atgtagaaaa ctaatcagct ttccatacta tctgaaaaga acatcaggac
     9061 aagacttttc cgtctatcct gatgttcttt tctacaaata aaaaaccgcc gagatggtta
     9121 gactcgacgg aaaaggagta tttactcgat tttgagtaag tacaaggttg ttgttggtta
     9181 taggtatgtc attatcataa aggattttga tcactctatc ataaaggtaa tattacattt
     9241 atattagata actctagttt ttattgttat caaagatttc ttttattttt tcaacaacaa
     9301 attagtcatt ttttggatgg cagctgtcaa ttggtctgtc gcttggtctt gttttgcttc
     9361 agatgtattg aatagatctt catctacccc tgattcaaga tcgatacttt ggatcatttc
     9421 ttcacagcct gctacatatg aaagataagc tttttcaaaa tttttatgaa tgcccattgc
     9481 ttttgctggt ggacgcaaag tactgacttt cataagcatt gcccgatatt tttttgttcc
     9541 ttcttcaaat aacaggatga tctcttcaag agtcgatttg tccagttcac ttgtttttcc
     9601 ttcatcaatt gcgtttctga tcacttcata tttcggatgc attccttctc cgattgcttc
     9661 tgtttccttg acgatttgat cgatggactg cacataatag ccaatattcg gtaacatgtt
     9721 cgttttccta ctttctccat tgttgttatt atttattcta cttgttttta tcctcaaatg
     9781 acaagtactt gaaaaaattt aatggtttct atacagctcg ccattcttat tcgtgctcaa
     9841 agaaaccatc tgctaaacta tattgtttcc ctgcgacaat gtcatattgg atcaattgat
     9901 attcgtcgta aatctcttgt tgcgagcgat tgaattgcgc ttctttatac cactgtccgt
     9961 tttgatagta cagttctcgt tcaaacgccg gaagttcctg ttctaatgat aaaagcagct
    10021 gataaaagcc atttgttgtc tgattcgtct gttccattag attcgctgca aaataaaacg
    10081 gactggtgat tgcatcgtga gtcgtttgct tttctaattc tcctttactg tcgaacataa
    10141 gaaattgtgt ttcatgcaat gtttgtttgt cattttttgc ttggatcgta tctgaataga
    10201 tccctggaag atgatccccc caaaaaacaa ctaaagtcct tctagaaaga ttcttcaatt
    10261 cttccgtgaa ctgcttcagt gccgtgctgc tataggaaat atcttgcaga taattctcta
    10321 agtcaagtgt cccacttcca tctgaagttt ttgcaggata gtctagatta tcatactttc
    10381 catcgtaggg catatgtgtc tgcatcgtga ctaaatgaat gaactgaggt gttttttctt
    10441 ccttgagaag tgtcattact tctttgtaag ctgactcatc agaaatatat ggattattct
    10501 ctatcgtatc cgtataggtc atcgtacgct cgctgatgaa ctggtcaaat cctaatgtct
    10561 gataaacatc ctctctttta tacatcgaag tattgtatgg atggatcgct gtcgtctggt
    10621 atgattgtgc gtttaacgct gaaacgatag aaggaagctg atccattttt ggtacaagca
    10681 tcgtgtaagg ggtggtgagt tgcgcattaa ataacgccat agaaaaacca gtcaatgctt
    10741 caaattcgat atttgctgtt ccaccgccat agttttgaga aagcatattg ccactatatg
    10801 tttgatcagc tacttcataa taatctgcca atggctcacc agatacttct acgccgttta
    10861 atcgactagg atctgagaaa ctttcactca tcacgaacac gatgttcggc gactcttcct
    10921 ctacagtttt atttttttca tcggctagct tctggtattt ttcagtgatt tctttgatct
    10981 tttctttcga atatccttca ggttcatcca ttggttctac cttcagattg tacaaaaacc
    11041 caccgataaa accagtgtta tagtagttca ttttctggct gtaaggtatc catagtgccg
    11101 ttttattata tgcttttcgc aacaaattat cgggattgtt gaaattactg atatacccta
    11161 gcagtccaat tgccacaagc agtgtcgtta atcggatcaa ttggaatcgc cttccttttt
    11221 tcaggcttcg aaacatgtac cagcaaaaca atcctatcac ggttcctgcg gcagctaaga
    11281 tcactgtaaa cagcatagtg ccggtcatat ctttcagcaa tccgatttca gtgatcatct
    11341 tcaaatcgtc tggataaatc ggttcttgcc gataactcat tttcatataa tttgcaaaac
    11401 ctaatacacc gatggctacg acatacgtaa gggaacctaa agggacagat cctgctagtg
    11461 caatcagaaa aataagaata atcagcaaga cgagacaggc taagaagaat ttttccgtat
    11521 gccaagaaaa agcaaatttc aacgcaaggt ctacggatag atcattttga caccactgca
    11581 aaaataaatt actaaaaaca acaagaaaaa agatactgat gattgctgct gttcctttta
    11641 aaatcttttt ctgtttggtc gaaagtctgc ttatatatct tttcaaatgc ttgctccttc
    11701 atttctatca ataaaagaat gttgcttctc ttattttagt tgtacgaaat tcgaaagtaa
    11761 agaacggaaa gaagaattaa tgaaatccaa atattctctt tctttatggc ctttctggtt
    11821 tctttaataa gcggaccttt cgtactccag aaattttttc tagctgttgt ttcacttgat
    11881 tgagctgttc aaaatcagta ctgtctatgt caatcaatat gtagacatgc tcattcttat
    11941 ggttacgcga tatatctgcg atcgctagct cgttctcatt cagcagagaa aatatctctg
    12001 ctaatatatt ggttttcttt tgataaaata tagtgaatcg aaacggcgct tggaaaaaca
    12061 ttcgtgcgga tggataattg accgcttctc taaccgtacc gaacaaaaga taatctttta
    12121 aggcttcaac tgccaaacgt ccgctgtctg ccaaagcttc ctgagtcgat ccccctagat
    12181 gaggcaacat aagtatacgg tcattttcta agaactcttc ttctggaaaa tcagtcaagt
    12241 atttagccag atgttgtttt tccaatgctt gcataacagc atatttatca acaatttcac
    12301 ttcttcctac attgataagt acagcatctt ttttcatttt ttctatattc gcctgatcga
    12361 tcaatccttt tgtatcttct gtcaacggca gcatgactac gatgtaatcc gaggtagaaa
    12421 gaactgtttc taaatctgct tgccgaacat aatccaactg ggcatcgcgg atagaataac
    12481 ccaagacatc catccctaag ctatagcaac ttagcgctac ttttgtgcct atcgctccaa
    12541 gaccaagaag accgatggtt ttgccttgca attctcgtcc cacataagca cttcttttgt
    12601 tttctgcctg ttctaaaata ttaggacctg ttagtgtttg taccatacga cttgcttcga
    12661 ttatcggacg ggaagataag agcaaacagc acaaaacaag ctcttttact gcattagcat
    12721 ttacacctgg tgtattcatg acgatcgtcc cgttttcaga agctttttca acgttgatcg
    12781 tgttcacccc aacaccagct cttgaaattg ctagcaaatc tggtgtaagc cattcatctt
    12841 tcactttcgt actacgaata actattcctt cggctggcaa atcatctatt attttgaaat
    12901 attccccttg taacttttct atctcttgtt ctggaatagc atcaatcaaa tgaacgtgat
    12961 acataaaacc ccctccttac taacaagtat ctcgaaattt ctgcaattag acaactgttt
    13021 accatttgac ttcctagaag caaaaagagc agttagagca gagtaggctg agtgataaaa
    13081 ataaggctat gacaagttac tgccataagc cttattttcg agagcaaaat attgagggtg
    13141 agatatcttg ataaatggtg ttccgttagc tgacattgat aataagacta aatttcaaaa
    13201 aagttgaaaa gcaaatttcg tcaatctctt cttatttctt aaaactaagt ggctttttca
    13261 caacctcttt ttatctaata accactgttt ccaactcaac taattttgcc cacgtttcaa
    13321 tttgatctgt tgttagattt agcgagacaa cggtatgatg accgccacct gcctgaatcc
    13381 atgaatggac accttcatgg aaattcggtt tagttttcca taacacacgt gcaacaggta
    13441 aattaggtgc ttcagtcgtt ggttcaaagg cttctacttc attgatcagc agtttataat
    13501 gcgtaccgaa atctgccatc gatacgacta ccccttctcc tgctttccca tcaaatacaa
    13561 gacgcgccgg atcttctcta tctcccattg ataaaggaga aacaacgatt cttggtttcg
    13621 tcgcagcaag cgtcggatct acttccatca tatgggattc caagattgct tcttggccac
    13681 tagctagctc gtaagtataa tcttccatga atcctgtatc tttccctcga gccatgattt
    13741 tcagcaaccg atcaatcgct gcagttttcc agtcgccttc cccagcaaaa ccgtatcctt
    13801 ctgccatcaa acgttgtaca gctagtccag gtagttgttt cattccatac aggtcttcaa
    13861 aattagtcgt aaaagcgtta tagcctcctt cttctaagaa acggcgaata ccgatttcct
    13921 gctgagcttg tacttttaca tgttcctccc atgtcttttc ttcataatca ccatagtcga
    13981 aggtatacaa ttccttgtac tcttcaaata attgatcgac ttcttcgtct gatacgcgat
    14041 cgattacttg tacaagatct ccgataccaa aataatcaac agtccaacca aattgaatct
    14101 gtgcttccac tttgtctcct tctgtgacac ccacgtttct catgttgtca ccaaaacgag
    14161 ccacttttat tccaaaactt tcattatagg ctactgcaac atccatccaa tcagcaatct
    14221 ctttttggat ctcaggtcgt ttccagtagc caacgacgat tttattttgt ttatttaatc
    14281 gagcattgat gaacccatat tcgcggtcac catgagcgga ttgatttaaa ttcatgaaat
    14341 ccatatcgat cgttttccaa ggaatgcttt cgttatattg cgtagctaaa tgaagcaatg
    14401 gtttttgtag aagctttgtc ccgcggatcc acatttttgc tggtgagaaa gtatgcatcc
    14461 aagtgatcac acctgctact tcttctcgat aattgacctc tttcatgatt tttgtgatcg
    14521 tatcagcagt aactgctaat tcttgaaggc ggattggata aggcaattgg ccattttcat
    14581 tcagttcatc gaccatttct tgtgcatgtt tctttacttc tcgcagtgct tcttccccat
    14641 ataaatgctg tgatcctaca acaaaccaaa attctttttc tccaatattc aacataatta
    14701 gccactcctt ttatttgttt tgtccgtaat acgcattttt tccgtgtttt cgtaaataat
    14761 gtttatctaa aatccgttga ggcagctctt ctgaaaatga attgagctgt ctggtaaata
    14821 aattcatctt gcatacttca tctagtacga ctgcgttcat taccgcactt tgcgcatctt
    14881 tcccccaagt aaatggaccg tgtccgtgta aaagtacccc tggaattgct aagggatcaa
    14941 ttttgcgttc tttgaatgtt tctatgataa cttttcctgt ttcatattcg tacccgctgt
    15001 cgatttcctg ttgtgtcaaa aatcgagcac aaggtaccga tccataaaaa gtatctgcat
    15061 gcgtcgtccc catcgcagga acatccaatc ctgcttgtgc ccagatcgtc gcccatgtcg
    15121 agtgagtatg gacgattcct ccgatttgag gaaatgaacg atataaaaca gcatgagtag
    15181 gcgtatccga agagggattg agtttacctt caatgatatg attgtccaag tccacaacga
    15241 ccatgtcgtc tgctgtcatc tcttcataac tgactccgct aggtttgata acgaaatatc
    15301 gtgtttcagg atcaaaagcg ctcacatttc cccatgtata tttgacgagt ccgtgtttag
    15361 gcaagtcaag atttgcttgg tatacttctt ctttcagttg ttccagcatt ttctatcact
    15421 cctttttctt gcaattgatg aatcgctgtt ttttcaatgt cgatcccctc acggtaacgt
    15481 ctcataaata tctgatagcc tgctatttct tctgctgaag gtgtgatggt gattccttgg
    15541 tcttgtccaa acacgtcttc tgccaagaag ttttctaaag acttcccatg tcgatccatc
    15601 atataggctg ctaataacgc gattccccac gctccacctt ctccggctgt gtccatcact
    15661 gtaactggtg cctccatagc agatgctaga atccgctgtc caacttctgg tgttttaaaa
    15721 atccccccat gccccactaa acgatcaatt tggacattct cagatttcaa tatatccatt
    15781 ccgattctca ttgcaccaaa agcactggct aaatggatcc gcatgaaatt cgctaaatca
    15841 aaacggctac ctggtgtacg tacaaataat ggccttcctt catttactcc tgtaatgttt
    15901 tcaccggaaa aataaccata agaaagtaat cccccgcaat ccgtatcgcc ttctagcgct
    15961 ttgttgaata ataccgtgaa taatttatct ctgcttatct ccatacccaa agaattggta
    16021 aattcttcaa aaatactgac ccaagcattg atatccgaag agcagttatt cgtatgaacc
    16081 atgcctacta gttctcctga aggtgtagtg accatgtcga tttccggatg gacattttct
    16141 agttcttttt ctaatacgat catagcgaag gcggaagtac cagccgaaat gttccctgtc
    16201 cctttggcta cactgttcgt agcgaccata ccggttcctg catccccttc tggcggacaa
    16261 accgggattc ctggtctcaa atttcccgta gggtcgatca gataagcccc tgattcgctt
    16321 aacttgccag cttcttctcc tgctactaga actttaggca aaatgtgatc caagtcaatc
    16381 gctacgtgcg ctttttcgat taactgacca aaaatatttt tcatttcttg atggtaggtt
    16441 ttcgttgaag gatcgatagg aaacatgcct gatgcatctc ctacgcctag tactttctca
    16501 ccagtaagct gccaatggac ataacctgca agcgttgtca aaaaattaat ttctttcaca
    16561 tgttcttctt tattcaaaat agattgatac aaatgagcga tactccatct ctgcggaata
    16621 ttgtattgaa atttttccgt taatattcgt tctgcttctt cagtgattgt atttcgccaa
    16681 gtacgaaatg gtactagtaa ctgttccttg ctatcaaatg ctagataacc gtgcatcatt
    16741 gcactgaacc caatcgaccc gattgtcgta agattctcgc catagatttc gaatacttct
    16801 gctgccattt ttcggtaact gacgcgcagt cctttccaaa tatcttctaa tgaataggtc
    16861 cagataccat tttctagttt gttttcccac tcataactac cagcggcaat aggagtaaaa
    16921 gatgaatcaa tcaatacagc tttgatccta gttgatccaa actcaatccc taaagatgta
    16981 cgaccatcta tgacatctaa catactttgc gcatttactt ccttcataat gatttaactc
    17041 cctccttctc gtcaaacctg atcaaaccgt ctttttagta gcgcttacaa tgattattgt
    17101 atacttttgt acgtacatgt caagcagtta tttacaagaa tacaataaaa aacacttgtt
    17161 ttgtacgtac ataagttata aaatgaaatt gcttacaaaa tctgatcaac cgccttctcg
    17221 aaattctaga aggagagaat acttatgaaa aaagaaaaaa aagtttcctc atcgtttatt
    17281 tatttttttg gcgcatttgg cggcattcta tttggatatg atattggtgt tatgaccggc
    17341 gccttacctt ttttacagca tgattggggt ttggcaggta aagcttcttt gataggatgg
    17401 atcacgtctt cggtcatgtt aggtgcgatc cttggcggct ctttatctgg tcttctttct
    17461 gataagcttg gacgacgaaa aatgattctt ctttctgcac ttatttttat ggctggttct
    17521 gtcctttctg catctgcccc tcacaatggt tcttattttc tgattgcagc aaggattctt
    17581 ctaggtttag ctgttggcgc tgcatctgcc ctagttcctg cttatatgtc tgaaatggct
    17641 cctgctagac taaggggaag attgtcaggg atcaatcaag tgatgattgc ttctggcatg
    17701 ctcctttcct atgttgcaga ttatttgcta aaagggttgc ctgaaacgat ggcttggcgc
    17761 gtgatgttag ggctcgctgc tgtccccgcg ctgattttat ttttcggtgt tttagctcta
    17821 ccagagtcac cgcgttttct tatgcaatct ggccgcttgg aagaggcaaa aagagtattg
    17881 aactatattc ggacaccaaa agaagctgaa caggaattcg aacaaatcca gctgaatgtc
    17941 aaacaagaaa agacaaccgg aacttcttgg catactttgt ttttagaaaa atatcgttcc
    18001 ttagtcattg ccggtattgg tgtagcagta ttccagcaat ttcaaggagc taatgctatt
    18061 ttttactata ttccgctgat cgtagaaaaa gcgactgggc atgcagcgag cgatgcattg
    18121 atgtggccga tcatccaagg aatcatttta gttgctggtt ctttgctctt tttggttatt
    18181 gcagacaagt tcaatcggcg aactttattg aaaataggcg gtagcgtgat gggactttct
    18241 ttcatcctac cagctgtttt aggaactgta ctggatgctc atacaaattc gttgttgatt
    18301 ctcttgtttt tatgtattta tgtcgcattt tattcttgca catgggctcc actgacatgg
    18361 gtaatcgtag gcgaaatatt cccactagct gtccgcgggc gagcatctgg cctagcctct
    18421 tcttttaatt ggattggttc tttcctagtt ggcttgcttt ttcctgtgat gactgcttct
    18481 atgtctcaag aaatcgtttt cggcattttt gggatcatct gctttttagg ggttttattt
    18541 attcaagaga tcgtaccaga aactcgtggg aaatctttag aggaaatcga acaaagtgca
    18601 tctaaaaaaa cttatcctaa acgtatttct atccatcatt cttaaagagg ttgtgacaga
    18661 agtggtcagc ttcaagaaat aagaaggaac cgcttagttt ccagtcacaa ggaacaattt
    18721 caaaaaacag cttctcatgt ttgatgaata ttgtgacttg tgaccagttg gtaggagaac
    18781 accgtttagt ggagaggtgg tgaaagaaac gttcagcctt gagcgatgac aaaagctctc
    18841 acaaacattg cgacgagtaa ccgtaggagc aataagaggc ggtaaaaacc cgtctcttat
    18901 ttttcgtttt ctttcactgt ttcccgttca actagtacag gttcatagag aatatttggc
    18961 tgtgcctgtc ctttttcaat cgttgtgacg atccaccgtg cggcatcttg tcccaacttt
    19021 tcttttggat gcgtcaatgt tgtcaatttt ttattcccta aatggcttaa agaagaatcg
    19081 tctgttccta cgatcgatag gtcatctgga atatgatagc cattttctgt caacttatct
    19141 agcattaaac tagctatttg atcgttatag caaatgatgc catctaaagc ttgttcttct
    19201 ttcaaccgtt gtactgtttt ttcagcgatt gtttctcgtg tttccgtggt gaaagtaatg
    19261 atatcttccg tcaaaaaacg cacattatac tgttcacacg cgctgataaa acctttcatc
    19321 ctgtattttc cttgaagatc gtcgattttt gtgatgaaca acagacgctg atgtcctttt
    19381 tttatcaaat attctgctgc cataaaaccg actttcacat catctaaaca aatataagag
    19441 gcatttaact cttcataagc agcattgatc atcaccatcg gaatgccttg ttctctcaaa
    19501 gcaacataaa gtgctaagtt tgggttatac tgattacttt tcgtaggctc aacgatcaaa
    19561 ccatctaccc cataagcaat cattttcttc aaacactctt tctccaattc gtgatcgtta
    19621 ttcgtactgg ctaaaagcaa tgaataccca gattctcgta attcattttc gattcctcgg
    19681 ataatcgacg gaaaaatata atcagagaga tatgtggtga tgacaccgat cgtttttgtt
    19741 ctgttcaatg gtgttttttc tttcttatag ctgtcatcta catatgtgcc cgaccctttt
    19801 tctttacgca gatagccctc atttaccaac acagcgattg cttgccgaat cgtatgtctg
    19861 ctgacgtcat atatttcttg cagcttgaat tctgagggaa tggttgtatt tacttcgtat
    19921 tcctcacgaa ggatcttttg ttttatctct tctgcaatat cttgatattt tgccatttgc
    19981 cttgcccctt tttatttgta cgtacatata tcataacgag tctgcaaggt ttattcaagc
    20041 aaaaatcaag gtttcttgtg ctcataaggc atacggaaca ggttcaccaa attctggaaa
    20101 tccttgctca ttccacgtaa agcattgcgc gttcgcgtgc cgattaggat tgtctaacgg
    20161 atcccctttt tggttcttct ctggtcgagc atgatagatc aaaatatcaa agcacccgtc
    20221 ttctgaaacc gtaaacgagt tgtgaccagg accaaattgc ttattttttt cagaagaaac
    20281 gaatactggc tccgataatt tatgccatga ttcttttaat aaaagattct tgtgctcatc
    20341 agcccacagc aggcccatgg catagttttc atctgttgca ctaccagaat acgtaatgaa
    20401 tattttccca ttgcgagtaa ggactgctgg accttcattt actgaaaaac cccgtttttc
    20461 ccattcatac tcaggtatag aaaggaggat ctgcttcccc tttaacgtcc aaggattatc
    20521 catttcggat agataaagat tagaattccc agtaatttca ggatcttgtt gggcccacac
    20581 ataatatagc tttccttgag aagagaaagt tgttccgtct aaactgaaag tttccctttt
    20641 cgtttttatt tgtccctttt ctatccaatc tgtattcatt ggatctatat gctgattttc
    20701 aagtacgaac atgcggtgat gatgagattc atcacgtata gttttatgat tgcttgcagc
    20761 aaagtaaata taccattttc cattgatata atggacttca ggtgcccaga tcaattcact
    20821 catcggtcct tcttcatgag cctgccatac aatcaggctt gcagcatgtt ctaattcatt
    20881 cagtgacttt gcccttctta attcaatcac ttggtagcct ggaaccgatc ctgtaaaata
    20941 ataataacca tctgtatgtt tatataccca aggatctgcc cgttgccgaa cgataggatt
    21001 attataaagt gtcttcatca taacaaattt cctcacattt tctttattct aattacattc
    21061 caagagtatt tttctatccc tacatttaat tcgtcatgtt tttctattga aaaatagttt
    21121 atttcaggtt ttattttttc tggttcttca cgtgtatttt tgtccaacag atcgctactg
    21181 gttaatgtgt agtgctctcc ttgatctagc aaattgaaat catgttcaat cgttatcttt
    21241 tgttgttcat ttgtgcgatt gatcaagaaa atagtcagct gattgccgtt atctacgatc
    21301 acactgtcca cataaggaac attttttatc ttttcacaat cataaacagg gcaaatgatt
    21361 tgttgtttca atacttgacc tttagcatat ttcgataagt gttgcatcac ataataagtt
    21421 ggattcctcc atgcttgtcc tttagcatct gtcagtatta aaggtattgt gtttactaac
    21481 aatgattgac aactaatctt tacgcgatct gcgtgtcgta aaatagccag ccctaatgaa
    21541 ccaaataata atgtatcttc catagtaaag taacaccaat cgatgggact accgacttca
    21601 aaatccctat gtcttttttc cggttgggca tgtacattcc attcatcaaa cgctagatac
    21661 attgttttct cacttctcaa atatcctttt acataatcac aagtagcaac gatttcttca
    21721 atttgacgat cgaatcgata agcttttgca aggtatgtcg ctgtatcatc acgctcattt
    21781 ttaggaggtt tagtcaagtc ttcttcatca tacttatcaa tatagttatg caaagctaaa
    21841 taatctacat tctcataagc ttcttctaat gcgatacgat cccattcagg gtacgatggt
    21901 aaacgtggcg tagagcttcc aactaagatt gtttcaattg aatcatccac taacttcatg
    21961 acttttgagg cttcatttgc taacctacca tattcataag cactcttagc ccccacttgc
    22021 catggaccgt ctagctcatt ccctaaacac cataatttaa cgccaaaagg ctttttttgc
    22081 ccatttttta ttcttaaatc actccagaag gttccttttg gaaaattaca atattctact
    22141 aaattttttg cttcatcaat tccttttgtt cctaaattca ccgtgaatat cggtttcgct
    22201 tgtacatgac tgatccattt aaaaaattcg tggagtccaa aagaatttgg ttctatacct
    22261 cgccatgcta aatcaatttt ttttggtcgt tgttctttcc tacctacagt atccacccaa
    22321 tcataaccag atgtaaagtt tcctccagga tagcgaatga cacctaattc taattccttt
    22381 acataagaga ggacatcttg tctaaacccg tcaccatcag ctgtttcatg gcccggttca
    22441 taaatccctg tatagacgac acttcccata tgctcaacaa atgagccaaa cagccgagaa
    22501 ctgatctctc ctaccgtaaa atcctctctg atttttactt tcacttcttc gtccaacagc
    22561 tttccctacc tttcatttcc tatagtcaag ataatagata caagtttcca tacgccacca
    22621 gcaaagataa aaataaaagg agggaaaaga aatatgagca gtacaaatcc taataacaat
    22681 acgcaacaaa gaatatttct caaaaaatca aaaaatactt tccatgcact ttgttcaaaa
    22741 atttgtaaaa atgtgatatc tttgttttca gaaatcacat atatccagga taaaagaaac
    22801 atggaataaa taagtaaaac tacatatcca ctaattgaaa gtacgattgt ctgttcttct
    22861 aataattgat taacaagaaa ataaataatg atcgttaaca gactaaggaa caagttctta
    22921 ccaaaatgtt gctttattcc ttgaaagaaa aaataaatga tatttcctga tccctcacct
    22981 ttccattgcg ccattacata aaaaacagca catgtagcag gcacgatagt aataacaggc
    23041 aaacacacga ttacccagag aatacctaat aagatgtaat cagaaaccac aatcaactgt
    23101 ccataaagtt tagtctcaac tattttcatc ttcctcactc tttcattcca gacatagctg
    23161 ttccttgaac aaaatatttc tgtgcaaaaa tataaaccac tagtaatggc agcattgaaa
    23221 taaccgttcc agccattaca ggtccataaa aagaaatgtt ctgccctgta aataaagcta
    23281 accctgctgg taaagttctc atttttgaac tacttgtcaa tatcaatgga taaagcaagt
    23341 cattccaact attcatcagc gtgaaaatcc ctaatgataa aagagtaggt ttgctcaatg
    23401 gcaacatgat tttcaaaaaa atattaaact cacttaatcc atcaatgcgt gctgcctctt
    23461 ccaaagaatc tggtaaagta ataaaaaagg aacgcatcat gaagatacca aatgcagttg
    23521 tcatacgagg aatgatcaaa cctgcataag tatcaagcat gccaagcaaa ttgacttcaa
    23581 taaatagagg aatcatgaag acttggaatg ggatcatcat tgtcagcaaa acgaaataga
    23641 acaaaactga ttttcctttg aaacgcatac gagcaaatgc ataccccgca agagaatcaa
    23701 agaatacaga agtgatcact gtccctccag caaaaatcac tgtatttttg atgtaatcta
    23761 ataaagggat cgttgtccaa accttaatat agttatctag ggtatagcta tctgcaaata
    23821 ttctaggcgg aaatgagata atgtcttttt cgtgtttaaa agaagaaata atcagccaca
    23881 aaaaaggaaa cgcaataacc aacaatataa ctagcatact aatcgttcga atgatttttt
    23941 taccactaac tgcttttcga ctattcacag taactgtaat cgagctttct ggtgccatac
    24001 aggtttccct cctatttcat ttgtttttct ttccgtgaca tgtacacatt agtggagata
    24061 gctatagttg caacaatgat aaacaaaacc gaagacagtg ctgatgcata gcccaattca
    24121 taaggtgctg taaatcctcg actgtaaata tattgaacag ctgtttctgt cttaaattgt
    24181 gggccaccag cggttgcaac atatacttgg tcaaacactt gcatcgaacc aatcagattc
    24241 gttaaaatac aaaaacctaa ggagggaacg atctgaggta gcgtaatgtt aaaaaactgc
    24301 tgtcgatttg ttgcaccatc catttgagca gattcataca aagagggaga gattccctga
    24361 atagcagtca agagaatcgt catagtaaca ccaaaattct tccaaacagt catgatggct
    24421 accgtcggca tagcaagggt tggatcgcga aaaaattgag ggatcggcaa gccgacttgt
    24481 gaaaacaagt aaggaatcgc ccctacattc ggatcaagca acatagaaaa aataatcgca
    24541 attgacgtca atgaacaaat aactggaatg taaaatgtcg aacgacaaaa acgattgaat
    24601 ctggtatttt tagataatgc tactgctgct aataaaccaa cgataatttg tgttggcgtt
    24661 tcgagtacgg tgaaatataa agaatgaatc aacgaattaa ctgctcgttc atcactaaat
    24721 atacgtgtga aattttcaaa acttacaaaa tttcttgatg taaagaaaat attcaaatct
    24781 gtaaaactaa tagcaaatgt tcctaaaagc ggtatgacag taaataataa taaaactaaa
    24841 aaagatggag ttaaaaacaa atacgcagca cgatcaggac gattccaata tttggtgaat
    24901 ccgttcatat agtccacctc atctttcatt ttgataaaat atacaatctt ggtttaaacg
    24961 aatctgaata agatatccga acaatcgctg tgatttttgc aataacacct atctgcaaat
    25021 actaggtcga tcattcagat atctgtatct tgccaacagt ctatcctgta taaagttctg
    25081 tactattttt gcagtaattg attgatggca tcatctgcat cgttcatgag tgtctcagga
    25141 tcttctcctg ctaataattt ttcaatcagt ggattgatga tatcgttatt gatcgcagag
    25201 atttgggtaa aacctggtaa atatggctct gcatagtcca tttgtttcga taattcagat
    25261 acaataggat cattttttac ttcttcatct tttgctactg aatccaagta ggcaggaaaa
    25321 ccattttcga tactccattt tttaccaatt tcagttgtat tccaatattt caaaaactca
    25381 tagacagctt cttttttatt attatctgta cttgccggaa tggcatatcc tacgccatct
    25441 aaaatagctg tttttttgtc tgaatcactc ccttgtggta aggtagtcac attataatca
    25501 atctcgtttg ctttcaatcc gttatttaac catggcccat tgatttcaat ggctagttga
    25561 ccagcattca ttaaattgtc catttcggcg ccagaaatcg attctggacc agtcttgtct
    25621 acatggatca ttttttggat cgcttttaac gttttaaggt tctcaggaga cgcaaattca
    25681 gacttagtat aatcatcgtt cactaattta ccgccatttg ctaacatcca gttatataac
    25741 ggtgcagttc catccttatt gaagacaaaa cctgatacat ttttactact atcagttaat
    25801 tttggtgcca tttctgtcaa ttcttcccaa gtagtaggtg gttgttcagt atctaatcca
    25861 gcttgtttaa acaaatcatt attccaatac atatacatgc cttgtacctg catggggaca
    25921 aaatactgtt taccttctac ttcacctaat tcaatagccg tttctttaaa atcagattta
    25981 tctactccat catattccca gaaatcgtct aatggttgta ccgctccgtt ttctacatac
    26041 tgtgcaaaat ctggataatt taatgcaata aagtcaggtg cttttttaga agaaatcgct
    26101 gtaggaagtt tttcattcaa attggcccaa gtcatgatat ccatttcaat tttgatgttt
    26161 ttatcatttg ttttattaaa ttcattaacg atttcttgca atatttcacc atcagatgca
    26221 gtaaacccat tccaaaaagt caaagtcact tctttatcag aatcagattc tgctgaactt
    26281 ccgccaccgc aagctcctaa aatacctgtt gcagctacca acaaagagcc aattaaccat
    26341 tttttacctt tcattttttg ttcctccttc atctgataaa cgacatctga ccacattcca
    26401 agatagcggt ttcaataaac aattgatttt cccatcttca gcttttacat ttgttgaatg
    26461 acttggtttt acttgttcac ttttaggaag attagttgct tttgtatcaa atccactcat
    26521 ttcagaaaaa tcaatcactc cctctaatga taattcagac aagtgaacag ttagattcat
    26581 ttcttcttct ccacggttaa ctgcaaatat gacaagttct ttgtcattta ttacggctat
    26641 actttctaaa tacggcactt cttcaaaatc ttccgtcgca tagtttgctg aacgaatttg
    26701 tgggcttaac acctctcctt gtccatacac agcagcttgc ataaatggat aatagatcgt
    26761 ctgtctccaa gcttctccgt ttgtttctgt cctgattggt gcaataacat ttaccaattg
    26821 agcaagacaa gcaatcttta ctctatctgc atgtttcaat aaagtgatta atagacagcc
    26881 taatagtaat gcatcttcaa aattatagtt atcttctagt aaaggaggag caatctgcca
    26941 aggcgctgct tgctcatctt gttccgctga atgataccaa atattccatt catcaaagga
    27001 caggttgatc tgcttcttac tatgtttctt cgccttgact gtatcgcaca tcgctgtcac
    27061 ttcagaaatg aaccgatcca tgtctaagga acgtgcaaga taatgagaaa gatcattttt
    27121 ttggttgcca taatattgat gcagagacaa ataatctatt tgatcgtacg cttgatcaag
    27181 aactgttaac tcccaatcac caaatgtagg catttgccga ttcgaacttc cgcataaaac
    27241 cacctcaatt gaatcatcta cgagtttcat cgccttagcc gtttcctctg ctaaccgtcc
    27301 atattcttcc gctgttttat gtccaatctg ccaaggaccg tccatttcat tccctaaaca
    27361 ccatgtctta attccaaaag ggctctcttg accattcgaa cgccttaaat cactccaata
    27421 agttccttta ggaaaattac aatattcgac taaattacga gctgcatcta ctccacgagt
    27481 acccagattg acagccatat tgatttcggc ccccacatgc tgacaccagt tataaaattc
    27541 atgaatccca acttcattcg tttcaattgt ccgccaagct aaatccaacc ttctaggtct
    27601 atttgctatg ggcccaatac tgtcttccca acgaaatcct gacacaaaat ttccaccagg
    27661 atatctaatc atgggaatgt tcaattcttt taccaagtga atgacgtctt ggcgaaatcc
    27721 acttttatta gctgatggat gatctggctg ataaatccct tcatatactg ctcgtcccat
    27781 atgttcaata aatgaactgt acaaccgcgg atcaactttg cctacacgct tgacactact
    27841 aatcaacatt gtggcttcca taactgacct cctttaattt atacatgaat aatacacctc
    27901 gttgaaagcg ttgtcaataa tataaatgtt aaacttactt attttttatc gattttaatt
    27961 aaccaa
//
LOCUS       DACEUF010000033        27677 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.034, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000033 DACEUF010000000
VERSION     DACEUF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.034"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            165..1904
                     /gene="yfcC"
                     /locus_tag="IXG20_001357"
     CDS             165..1904
                     /gene="yfcC"
                     /locus_tag="IXG20_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1609564.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2061..3245
                     /gene="iadA"
                     /locus_tag="IXG20_001358"
     CDS             2061..3245
                     /gene="iadA"
                     /locus_tag="IXG20_001358"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ1609565.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3356..3559
                     /locus_tag="IXG20_001359"
     CDS             3356..3559
                     /locus_tag="IXG20_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1609566.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3660..4322)
                     /locus_tag="IXG20_001360"
     CDS             complement(3660..4322)
                     /locus_tag="IXG20_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609567.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4335..5837)
                     /locus_tag="IXG20_001361"
     CDS             complement(4335..5837)
                     /locus_tag="IXG20_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1609568.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6385..8064
                     /locus_tag="IXG20_001362"
     CDS             6385..8064
                     /locus_tag="IXG20_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1609569.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8191..8669
                     /locus_tag="IXG20_001363"
                     /pseudo
     CDS             8191..8669
                     /locus_tag="IXG20_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
     gene            8888..9568
                     /locus_tag="IXG20_001364"
     CDS             8888..9568
                     /locus_tag="IXG20_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ1609570.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9576..10907
                     /locus_tag="IXG20_001365"
     CDS             9576..10907
                     /locus_tag="IXG20_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1609571.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10922..11884
                     /locus_tag="IXG20_001366"
     CDS             10922..11884
                     /locus_tag="IXG20_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ1609572.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11995..12333
                     /locus_tag="IXG20_001367"
     CDS             11995..12333
                     /locus_tag="IXG20_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ1609573.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12412..13119
                     /locus_tag="IXG20_001368"
     CDS             12412..13119
                     /locus_tag="IXG20_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ1609574.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13222..14703)
                     /locus_tag="IXG20_001369"
     CDS             complement(13222..14703)
                     /locus_tag="IXG20_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1609575.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            15012..15548
                     /locus_tag="IXG20_001370"
     CDS             15012..15548
                     /locus_tag="IXG20_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1609576.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15626..16996
                     /locus_tag="IXG20_001371"
     CDS             15626..16996
                     /locus_tag="IXG20_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ1609577.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17063..17398
                     /locus_tag="IXG20_001372"
     CDS             17063..17398
                     /locus_tag="IXG20_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609578.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17479..18336)
                     /locus_tag="IXG20_001373"
     CDS             complement(17479..18336)
                     /locus_tag="IXG20_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ1609579.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18443..18958
                     /gene="tadA"
                     /locus_tag="IXG20_001374"
     CDS             18443..18958
                     /gene="tadA"
                     /locus_tag="IXG20_001374"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ1609580.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            18981..19065
                     /gene="ffs"
                     /locus_tag="IXG20_001375"
     ncRNA           18981..19065
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IXG20_001375"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19275..20660
                     /locus_tag="IXG20_001376"
     CDS             19275..20660
                     /locus_tag="IXG20_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ1609581.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTDIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20697..21374
                     /locus_tag="IXG20_001377"
     CDS             20697..21374
                     /locus_tag="IXG20_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ1609582.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21382..22146
                     /locus_tag="IXG20_001378"
     CDS             21382..22146
                     /locus_tag="IXG20_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ1609583.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22148..22807
                     /locus_tag="IXG20_001379"
     CDS             22148..22807
                     /locus_tag="IXG20_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ1609584.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(22837..23346)
                     /locus_tag="IXG20_001380"
     CDS             complement(22837..23346)
                     /locus_tag="IXG20_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ1609585.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23458..24456)
                     /locus_tag="IXG20_001381"
     CDS             complement(23458..24456)
                     /locus_tag="IXG20_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1609586.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            24657..25241
                     /locus_tag="IXG20_001382"
     CDS             24657..25241
                     /locus_tag="IXG20_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1609587.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(25318..26493)
                     /locus_tag="IXG20_001383"
     CDS             complement(25318..26493)
                     /locus_tag="IXG20_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1609588.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            26575..26955
                     /locus_tag="IXG20_001384"
     CDS             26575..26955
                     /locus_tag="IXG20_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1609589.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            27029..27391
                     /locus_tag="IXG20_001385"
     CDS             27029..27391
                     /locus_tag="IXG20_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609590.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         8993 a         4675 c         5774 g         8235 t
ORIGIN      
        1 gaaagagaaa aaaataatca tacataggag aaaaaaaaca aaatagaaaa aagtgatttt
       61 ctaataaatt attcatgtgg aaatgtcatt tttactgtat aattcatttg attatccatt
      121 ttttattcat caaaattact gaaaaaagaa aggaagaaat aaatttgaag aaaacaaatt
      181 cttcaggaaa accgaaacgc gacttcacta aattatcgac accccatacg tatgtcatta
      241 tttttggtgt agtcatcttt gcatggatac tgacttttgt cgttccagca ggaaagttca
      301 gcacgcaaga tattgaatac aaagatgcaa atggcgaaac aagtactcgt actgttttac
      361 gtcaagattc cttccgttat gcttacgagc tggataagtc gtatgtgttt gatcaattag
      421 aagaactgca agatcatcca gcagaacgtg aaaaactgga tgttccggaa aaaggattgg
      481 aaaaagtcat tgctgacgga gaaaaaaatc ttacgcagga aaaactagat gaaatctcct
      541 tgacagatga tgtgctttac gatcagtacg gcgaaaatat ctatgatact tctaaaaaat
      601 tgcataaaac agccaaaata tggggaacag atgattttgg cggctttggt ttcttaaatt
      661 ttgtttttga aggactagta tctggagata agtatggatc tgctgtaggg atcgctgcat
      721 tgatccttgt tgttggaggt gcttttggta ttattatgcg taccggagcg atcgacgcag
      781 gtatctatgc gtttatcagt aaaactaagg gactggagcg tttagcttta ccgctattat
      841 tctttgcttt ttcattcggt ggcgctacat tcgggatggc agaagaggta attccattct
      901 caatggtgat ggttccattt gtcattgcgt taggatatga ctcgatcgta gcagtaaccg
      961 tgacttatgt cgcatctcaa gtcggaaatg cgacctcttg gatgagcccg tttagcgtag
     1021 cggtggctca agggattgct ggaatccccg tgttgtctgg tgcgacattc cgattgatta
     1081 tgtgggtagt tgttactgca ttagccgctg gttatatgat gatatatgca gaaaaaatcc
     1141 gtaaaaaacc agaaagttcg ttgacttaca aatcagatga ttatttccgt tctcatatca
     1201 aaaagacatc tgatgaaaat aaaaaattca tgcttgggca taaattgatc ttattagaga
     1261 tgttagttgt ccttgtctgg atcgtttggg gcgtgactca aaaaggctat tacattccag
     1321 aaatcgcttc tcaattcttt gtgatgggat tagttgctgg aatcattgct gttcttttca
     1381 aattagatgg aatgaaagtc aatgacatcg cttcttcttt ccaatctggt gcagctgatt
     1441 tagctggtac agcaatcgta gtaggtatgg ccaaagggat tcttcttgtc ttgggaggat
     1501 ctgatgcatc tgttccttca gctttgaata cgattttgca tgggatcggg acggcgttga
     1561 ccggtgttcc agctgtcatt ggtgcttggg cgatgtacat cttccaaagt ttgttcaact
     1621 tggttgttac atcaaattca ggacaagcag ctttgactat gccaatcatg gctccactgg
     1681 cagatttagt aggtgtatcc agacaagtag cagttcttgc ttatcagctt ggttcaggtt
     1741 ttatggacgc ttttacaccc gtatcagcca gcttgattgg tgtgctaggt gttgcacgga
     1801 tcgaatgggc aaaatgggca agattccaaa tcaagatgca aggattcctg tttgttttag
     1861 gaactatttt catcatgatt gccattgcaa taggtctgca gtagtactgg agacctgtaa
     1921 ataaaaaagt aatccaaaca gtagataagg actccaagaa ccgtcatttt acggtaaaat
     1981 caaaggagta aagatcctat cgtttggatt attttttaag atcctatcat cagttatact
     2041 taaagaaaga ggaaaaatgt atgaaaatca ttcgacaaat tgaagtgttt gcaccagatt
     2101 acttaggaaa aatggatgta ctgattgccg gtgggaagat tttggctgta gaagaacagc
     2161 tagaaggcgg ctacgaagga gtggaagtag aagaactttc aggtgaggga aaagtactaa
     2221 ccccgggttt cattgactgt catttccata ttttaggtgg aggaggagaa ggcggttatc
     2281 aaaatcgaac gccagaagtc acattgagcc aattgactac agctggtgta acgacagttg
     2341 tcggttgttt gggaacagac ggagaaggta gagatatgac tgcgttgatc agcaaagcga
     2401 aaggtctgga agcagaagga atctccactt atgtatacga aggttcgtac cgattgccgg
     2461 taaaaacagt aacagattct atcatcaaag actttttgac aattgataaa attatcggaa
     2521 tcggagaaat cgcagtatcc gatcaccgtt cttctcagcc aagttttgaa gagttcgctc
     2581 gtgcagtagc agatgctcgt gtaggaggca tgttatccgg aaaagctggg atcatcaatg
     2641 tccacttagg cggaggaaaa agaaagctag agctattgac acgaatcgta gaagaaacag
     2701 aaattccgat cacacaattt ttgcctactc atgcaaatcg cacaccagaa ttatttgaag
     2761 cttgcgtggc atttgccaaa cgtggtggca cgattgactt cactgctagc gaagatcctg
     2821 atttttggga gaagacagat ggtgaagttc gcttcagcaa agcactgaaa cgattgatcg
     2881 aggaagatgt ttctttagat aatttcacga tgacctcaga tggacaagga agcttgcctt
     2941 attttgatga aaacaatcat ttcctagggt taggagtagg aagtgccaag gcattgctag
     3001 tagggatcaa agaagctgtg caaaaagaaa gtatcccttt ggaaatcgcc ttacgagcta
     3061 taaccagtaa tccagcacgt atattgaaat tggataaaaa aggaaaaatc gaaattggtg
     3121 ctgatgcaga tctgtgtatc ttggataaag aaacgcttga tattgataca gttatcgcta
     3181 aaggagaaat aatggtccaa gaaaaagaag tcaaagtatg ggggactttc gaaaaaagtt
     3241 tttaatcaac agctctaact atctttttta gaaaacgagc gttctgtttg tctgtatctg
     3301 ttgttgatgt taccctgaag gtagatcaat aaacaaacta agaggaggaa gcacaatggc
     3361 agatttaaaa ggacgttcta aagatatgaa agataaagta gtcggtgaag gaaaagaagc
     3421 atacggcaaa gcaacagacg acaagagcaa agaaacagaa gggaaagcac aatcactagg
     3481 tgctgacatc aaagaaaaag ctcgaaattt aggcgacgaa gtcaaagaca agttttctcg
     3541 aaaagacgaa aaaaaataaa aaccacttat tactaattga atcaagcaaa taaaaaatcc
     3601 gaaccatatc tgcgagacag atatggttcg gattttttat ttgatcatta tcctgttttt
     3661 taaacttcga ttttttgaaa ttttttattt tttagttcaa agacttcatc acagcttttt
     3721 gctacttcaa tctcatgagt cacgatgata atacagcgat tttgttcatg ggcgatttct
     3781 tggaaaagtt tgacgatatc ttgtgaagtt gtttcatcta gatttccagt aggctcatcc
     3841 gctacgatta gttgatgttc acagcagata gcacgaacga tagcgactcg ctgctgctga
     3901 cctcccgaaa gtttggtcac ctgttttttt gctaatattt catcaattcc tacacgagcc
     3961 agtcgatcta aagcaacttg tcgataattc tcttgcttgg tcttagtgat ttccattgct
     4021 gctatgacgt tgtctagagc cgataaataa ggaagcaagt tgtaagcttg gaaaataatc
     4081 gagacatcat ttttacgata acttcttaac ccgattttgt tcagtgaacg atcttgatag
     4141 tatacctttc cttcttttgg tgcatctagt ccagaaataa gcgaaagaaa agtcgttttt
     4201 ccagaaccac ttgctcctaa aatagcgtac atttttccag cttcgaaatt cagttctaca
     4261 tctttataaa gcgcatcttg cggatcttga taccaaaatc ccaattgttc tgtccgtaac
     4321 atcaaattcc tcctttagtt taataggatt tttcttgggt ttaatcttaa tatccctata
     4381 gaagctaaaa agacagaaaa cagactgatt ccaaatgcta ctccggctaa tagtcccaat
     4441 tgtgcaggtt tgacattgat ttccaattcg ctgacttgat cagaaacagc aaatggatta
     4501 ttcgtggttc cttgttgacc atcgcgttga ccgccttgtt cttgtctgtt acctggcatt
     4561 tgtccaccgc caggagcttg ctggttttgt gtcgtggttt ctgtctgttg gtcaagtagc
     4621 tgctgtccca cggcattcgc gacgacgttt ccgctaagcg aagcaactcc taatgcaaga
     4681 atcatacaaa tagccacttc ggtaaacaat tggagaatga ttttacttct ggattctcct
     4741 aaagatagca agacgcctaa ctcatgccga cgctcccgaa tggtaatcat aatgatcaga
     4801 gtaagtatga taataccggc tacagccact agtaaaacaa catttttaga aaaacttgcc
     4861 acgttgttta aaggttccaa cattgactga tacatggaat catttgattg taagctaaat
     4921 gtatctgtgt cgatcagctt ctcagcttct ttgagaaact catccatttt atctggatca
     4981 gatagagtgt aaatagcaga atctatcgtg ttatcgtcag atgtcccatt tagttcattt
     5041 gcaaaagtgt aagaagtgta aagtgtattc gacggattca tgaagttgaa tttcataccc
     5101 atactgccgc ttgtttcgct ggattcataa attcctttga tcgtcatttc ataagtggta
     5161 tcttcatctt cagtactcgt gattgtgaaa gtatctccga ctgccaggtc atttgtttct
     5221 gctagagtag aatcaatgac tacattgttt gtgcctttgt cgctgtcatc tattccttca
     5281 ccatccgtga tttttgctgt tccatcagaa aaattactat tttgtgcagt tgctgacaca
     5341 ccactgattt gaaagtctgc ttgagtcatt tgcggattcc cgcccattcc tccagcaggt
     5401 tcaccgcctg catcagtagt ttctgaactg tcttcacttg aatcagaact agtgatggca
     5461 gttattccat cttttgccat agctgaggcg gaactttcaa aggaataact ctttacatta
     5521 tctaattcag ctattttttc cgcgtccgtt aaggaaactg gtgtaagaga aaaactgcct
     5581 ggatctggac gttcttcagt ggtagaaccg cttgaagaag tttgttcctg ttttttaaat
     5641 gaagcttctc gattggcact taaagtcact gttgctccaa cttctttttg tgcttgattc
     5701 gctgcttctt ctgcagcgct tcgaatcgtg aggccagcta gcacaaaaat aagaatggct
     5761 gtgaacacgg caaacaataa caatgaacgt ccccatttta cttttgtact tttccatgca
     5821 cgtttcataa aattcatatt ttgcatccct cctagtagat gcatttgata atagacaacc
     5881 tatcttaaag ctagcttaaa tcttatgctg tttgcacacc atcaacatga ggttctgtat
     5941 atataaaaag tcactatagt tattgcaaaa aatgtggtaa aatgaaaaaa gtatgtttca
     6001 aaccgacgaa gaagaaatga aagaggaata gcaaaagaaa tcctagatgc tacagaggga
     6061 tttttacgtg catagttgtt ctaacaattg ctgatactag cgatttagtt tatttgttgt
     6121 ataataatgg ggaacagctg aatacttata taagtattct ggctatagcg tttcgcaaaa
     6181 gatccatttt gttaatgaag tatatccaaa ataaaatgcc catccaactg tcagaacgcg
     6241 gatgtgctgg taattttcct gctgattctc cataagttga gaaatagtga ttttgcatat
     6301 gcttcattgg aatggctcat atatttagat aaagaagaaa atcgacatct ttaaacttgg
     6361 atgtcataag aagaggtgaa gtttgtgagt accattaaaa ttatcccatt aggcggcgtt
     6421 cgtgaaaatg gaaaaaacat gtatgtcgca gaagtgggcg aggatatttt tattttagat
     6481 tgtgggctaa aatatcctga aaatgagttg ttggggatcg acgtcgtaat ccctgatttt
     6541 acgtatttag aagaaaacat tgatcgtgta gctggtgtct ttttaacaca tggacatgca
     6601 gatgcgatcg gggcacttcc ttacttgttg tcaaaaattt ctgtaccagt attcgggaca
     6661 gagttgacga tcgagttagc aaaattgaat gtcagtcgta atgaagcagc caaaggattt
     6721 aaggatttcc atgtggtaga tgagcataca gaaatagact ttggtgaaac agtagtgagc
     6781 tttttccgta ccactcatac gataccagat tcagtgggga tcaatctgaa aacaaaagaa
     6841 ggtagtatcg tttatacggg agattttaaa tttgaccaaa cggctatccc aatgtatcaa
     6901 acagattatg cacgattggc ggaaatcggc aaagaaggag tcttagcctt actaagtgaa
     6961 tcggcaaatg cagaaaatcc tcagcctgta gcttctgaag cccaaatagc agatgaagta
     7021 tttgatacga tcaaatattg ggaagggcgt atcatcgtcg cttgtgtagc aagtaatttg
     7081 caacgagtgc aacaagtatt ggatgcagca gaaaaagcac aacgaaaagt tgtcttgact
     7141 ggtcaagatt tcgaacgaat tattcgcaca gcgatgcgtt tagaaaaact tcaattacca
     7201 agtgaagatc tgcttgttac gttgaaagac atgaaaaact atgatccaga agaactgatc
     7261 attttagaaa ctggccgaat gggtgaaccc atcaagtctc tgcaaaaaat ggcaaacggt
     7321 acacaccgta atgtcaagat tgaagaagga gatttggttt atatcactac tacgccaagc
     7381 atcgcgatgg aaaccatcgt ggcgaaaacg gaagatatca tttatcgagc tggtggaaca
     7441 gtgaagttga tctctgagaa tatgcgcgtt tctggccatg ccaatcctaa tgacttgcaa
     7501 ttaatgatca atttgatgaa accaacatat tttgtgccag ttcaaggaga gtatcgtgaa
     7561 ttagctgctc atgcagattt ggctcatgca gtagggatgc catacaaaaa tatctatatt
     7621 actggacgcg gagacgtgct ggaatataag aacaagcgta tgagtgttgc aggaactaca
     7681 agtgcagaaa atgtaatgat tgatggatta ggcataggtg atatcggaaa cattgtttta
     7741 cgtgatcgaa aaatcttgtc agaagacggg attttcgtag cggttgtcac tatcagtcgg
     7801 cgagaaaaga aaatcatttc aaaacctcaa attacttcac ggggattcgt ttacgtaaaa
     7861 gctagccgtg atctgatgaa agaaagcagc aatatgattg aaacaatcgt tgagaaacat
     7921 ttagaaagca atgattttga gtggagcaaa ttaaagcaag acatccgaga tcaattaggc
     7981 cgttacttat ttgaacaaac aaaacgccgc ccagtgatct tgccagtcat tatggaagct
     8041 actcaacgtc gaaataaaaa ataaacaaaa agagtaagat gaacctctct atagagtgct
     8101 cgtcttgctc tttttttgcg aaaaacaaat aatttttgaa tacagcatcc ctcgctttat
     8161 aataaacgat agaataaagg gagtgaatca atgatgagtc aaacaaaagt cgaacgttat
     8221 ttagaagaac aaaagatcag ctatcaacca cttgatttta gtcatgtggt agaagaatct
     8281 ttcacagaag aactacaaaa aagagggatt gatccagctt tgatatgtaa aactctcgta
     8341 gtgaaaggaa acaaaactgg ggtgatcatc gcattgattc ctttgaacga tcacttagat
     8401 tacaaaaaaa cgagacaagt gacgaaagat cgtaaagttg gttttcctgg aatggatttt
     8461 gtattaatga tacaggatat ccccacggag caaacacccc tattggaata aaactcgcac
     8521 atcctgatta ttgtttttta gcagacagtt ctttaaaaga aaaagaagaa gtaattgtgt
     8581 caagcggtga aatcgggaga agccttcgga taaaagtagc cgatttagaa aagatcgtcc
     8641 atcctattta cgctgattta attagttaag ggacgagaag agaagctaag cagagcagag
     8701 acctcaaact tgagattata tctgcttggt acttggtgca tcctgtatta tctgttacaa
     8761 ttgctttgta catactgtac tatcaatcca atgaaaatat atttagcagc tatatttttt
     8821 cattgatgtg caagtgatga aatcgttttt aataaaaaag atacagagaa aacgcgaggt
     8881 gtaaaatatg aaaattacgt atcatggaca ttcctgttta acaattgaaa tggaaaatgg
     8941 ccaacgcgtg atgattgacc cgtttattac aggaaatcca ttgacagact taactgcaga
     9001 agaagtaaag acagattggt tgctgattac tcatggtcat agtgaccaca taggagatat
     9061 gattcctatt gcaaaagaaa atcaagccac aattatcagt attgttgaga ttgctgatta
     9121 tgcgaacagc agaggagtag attcattcgg tatgaatatc ggtggaaaac atgcgtttcc
     9181 attcggcgtg gtaaaattcg tacatgcaca gcatagttca agctatgaag tagacggtat
     9241 cgttcagtat atgggcgagc ctagcggaat tattattcaa gcagaaggaa aaacgattta
     9301 tcatgcaggg gatactgctt atttcagtga tctaggattg cttgcagaag aattcgatat
     9361 tgatgtcgct ttcttgccga ttggcgataa ctatacgatg gggccagaag atgcagcaaa
     9421 ggctgcgcaa acaattcgtg ccaagaaagt agttccaatc cattacaata ctttccctgt
     9481 cattgaacaa gatcccgaat cttttgtaga gttactggaa gatagtgaag gaaaaataat
     9541 gagcgtctcg gaaacgctgg aagtgtaggc agaaaatggc gacaaaacat gatttgattt
     9601 tagaatatat tgaaagttta cctgttggca atcgaatttc tgtaagaagc atcgcaaaag
     9661 aattgaatgt cagtgaagga acagcgtacc gtgcaatcaa agatgcagaa aatgtcggac
     9721 tagtttcaac aattcaacga gtagggacca tccgtatcga acgcaaatta aaaaaacata
     9781 ttgaacgttt gacatttggc gaggtcgtca ggatcattga aggggacgtt ttaggcgggg
     9841 cggcaggatt agacaaggtc ttgaataaat tcgtcatcgg cgcgatgact aaacatgcca
     9901 tggaacgcta tattacgcca ggatcactaa tgatcgtcgg caaccgggcg gaagtccaaa
     9961 aattagcatt ggaagatggc gccgcagtat tgatcacagg aggattcaat acgactgctg
    10021 agattgcaaa gctagctgat gatcttgaaa tgccgatctt acgcacctct tatgatacat
    10081 ttacagtcgc aaccatgatc aaccgagcgt taagcgacca attgatcaaa aaagatatca
    10141 tgcttgtcag tgatatctat atgccagctg aaaagactca ttatttgcag cagacagata
    10201 cgatttctga ttatcagcgt ttgtcagaag aaacgcagca ttcgcgtttt ccagtcgtca
    10261 accgccatca tcgtttgatg gggattgtta cggcaaaaga tgttttaggg aaatcgccaa
    10321 accagatcat cgatcgggtg atgacgaaag aaccaatcag tgtaaagaaa acaatgagta
    10381 ttgcctctgt tagccatcaa atgatctggg atgggctaga agtcatgccg gtcgtttccg
    10441 atgacttgac attggaaggc ttgattaccc ggcaagatgt aatgaaagcg atgcagcttg
    10501 tccagcgaca accgcagatt gcagatacga tttcggatca gatatctgga aatatccata
    10561 cgattgatac tgaccgtgaa ggaaatcgtt tggaaaaccc taagttcaaa ttcgtagtag
    10621 caccacaaat ggtcaatggc gttggaacca tttcttttgg cgtacttagc gaaatcattt
    10681 ctgatgtcgc acaaaaaaca atggtgatga accaaaaacg aaatatcttg attgaacaag
    10741 tgaacctaca ttatttgcgg ctgatccaat tggaaagcga attagacatt cgtccacgtg
    10801 tattggaaat cggcagacgt tccgctaaac tggatatcga agtgtttatt gaaaacacca
    10861 tcgtttccaa agcaatcgtt gtttgtcaag taatggaacg ttcttagaaa gcaggagtga
    10921 aatgacagta cagcaagaaa tagtagatac aataaaaaaa tacgataaaa tcattatcca
    10981 caggcatttg cgccctgatc ctgatgcatt aggatcacaa gtaggattag ccgaactatt
    11041 acgggaaagt ttcccagaaa aagaaatacg tcaagtaggt gaaacgattg aagggcttcg
    11101 ctttttagca gaaatgcaag aagttgaaga tgtcttctac caaggagcgc tagtcatcgt
    11161 gactgataca gctaattcac ccagaatcag tgatcaaaga tatcagttgg cagaaaaact
    11221 gatcaaaatc gaccatcatc ccaatgatga cccttatgga gatcttgtgt gggtaaatac
    11281 aaaagcaagt agctgtagtg agatgattgc agaatttgct ttgatgtttc ctgaatcttt
    11341 aaagatgaac gttaaggctg cacggttact ttatgcagga atcgtcggag atacagggcg
    11401 ctttttgtat ccctccacga cgtcccgtac attagagatt gcttcgatat tgagaaatta
    11461 tccatttgat gcaagtgctt tgaacagaga gattgaacaa atgccgatga aagtcgctaa
    11521 actttccggt tatctttatc aaaatatcca ggtggatgaa aatggtgcag gaagaataat
    11581 ccttccgcaa gaaattcttg ataaatatgg aatcgatgat tcagagacag cttcgatcgt
    11641 ttcacttcct ggagtgattg acgaagtgct ggcttggggg atttttgttc agcagccttc
    11701 agggtattac cgcgtacgtt tacgttctaa ggggccgatc atcaatgaat tggctaaacg
    11761 ccatcatgga ggaggccatc cattagcaag tggtgcgaat gcaaaagatt tagcagaagt
    11821 agaggaaatc tataaagaga tccaagatat atgccaatcg tataagagca gaaaaacaga
    11881 atgatacttc gttcgaaaac gtgccttttg tagtaaaaat cacaaatgta tcttttcaag
    11941 atcttctttt tccgttacaa tagtgctcac taaagatttt aaaggagaaa gaagatgaca
    12001 ttaccaaatt gcccagaatg tggctcagag tacacatatg aagatcgagg cttgttgatt
    12061 tgcccagaat gcgggaacga atggaacaaa gaagaacaag aaactgtcat agaagacgga
    12121 ttagtggtat tagatgcaaa tggaaaccgt ctgttagagg gcgatagcgt gacagtgatt
    12181 aaagacttga aagtaaaagg tgcgtcaggt gccattaaac aaggaacaaa agtcaagaac
    12241 atccgtttag tagaaggcga tcataatatc gattgcaagg tagaaggatt tggaccgatg
    12301 aaactgaaat cggaatttgt gaaaaagaat taaagaatat tcaaggtctt ttcacattct
    12361 tttcctacta ctaatgataa aataaagtaa acgaaaagga gtgaaacgaa aatgtatggt
    12421 tatggaatgt atggtttttt agatccgaca tttcttttgg tcattcttgg attagttatt
    12481 tccggaattg cgtcagcgta cgtcaatagt actttccgaa aatacgatca agtgagaagc
    12541 aaaaacaatg tgactggtac acaagcagca caatatatat tacaaagtca acagataaat
    12601 aatgtcggtg ttcaacaaat tgccggagat ctcacagata actacaattc aggtaataag
    12661 atgttgagtt tgtctcaagc aactgcacag tctacttcag ttgctgcgat aggtgtagca
    12721 gctcatgaat gcggacatgc ggtacaagat gcttcgggtt atgtcccatt gaagattcgt
    12781 gcagcacttg ttccagcagc taactttggt tcgatgattt cgttcccctt gattatggta
    12841 ggtgtattgc ttagctggaa ctctacgtta atcaatatcg ggattttcgc tttttcttta
    12901 gcattacttt tccaattggt gacattacca gtcgaattta acgcgtctcg gcgagcaatc
    12961 caaattttga gtgaaggcgg actactgacg gaagaagaag taccgatggc aagacacgta
    13021 ttatttgcag cagcactgac ttatgttgca gcagcgcttt cgacattcct gcagctgttg
    13081 cgcttgatct tattgttcgg cggcaaccga agagattaaa ataaagaagt tgtgaaatca
    13141 gcgctttgat ctgagctaat accgagagtt agctgattga ataccattta tttggaataa
    13201 gaggatgtga caagaatttt gtcacatcct ctttgttttg ttctcataac cttttttcat
    13261 atacagttca tgaaccgctt ttattaactt gccaaataac actgtattgt gcgttgtatc
    13321 cagatagaaa gtctccgttt catctgaaat tttctcatgc tgatctgtaa tgatcaaatc
    13381 agcatgttct gaatatgcgg taattttaac agcatctttg cgaaataact gctgcaaatg
    13441 gctttgaaga tgataatttc ctgtaaactg tttggttatc tgcaaatgga tgagtaactg
    13501 ctctttcgtt tctgtttgta gtaagtgttg gatcaattga ctaaggtatt gactgatttg
    13561 gtttctttcc ccattttttg aaaaaattgt atcgattttt tctcttaatt tcttttcctg
    13621 tataaaagaa ctagaatgat tggacgtggc ttctggaaag caaagtgtag aaaaactact
    13681 gctggattca cccaacagtt cgaaaaaaac aactgccaac acaagaaaat aaaaataaat
    13741 tggtatttta ttttctaaat ggttgagagg aaactgttga gttaagtaag tcatcaattg
    13801 accgacttgt cgacaaataa ggtttttgct tgtggaaaat aaaaaaccaa tctcctgttt
    13861 ttgtcgaaaa gagatttgat ttggaaaaaa acaatagaaa aaataattaa agtaggctct
    13921 ctctgtttct gtctcttcac aagggcgact cactgaaaaa gaagtaaagt attctgaata
    13981 atcatgtgta tgctgcaata agtccatcaa ttcagtgatt tcagaagaag tttttggaaa
    14041 atatgcctgt tgtttgtttc gtaatatgaa taaagttaac agcaagtaga tatttttttc
    14101 ttgttttgaa attttttcta tttgctcatc tgtactgttc atttttagtg catttaattc
    14161 aagtgaggaa aatggccaat cgttacctcc aaatgtatct gaaagaaaaa agtaaagata
    14221 gagacggata gataactcat ttccttcaag agtacagcaa ttttttacag ataggatccg
    14281 taaatggaat ttttttaagg aatgattgag ctgttttgtc agtttttcga gataggaggg
    14341 cgatatataa agttgttcaa gtaaatattt ttttggtaca gcagtttgtg gataattgat
    14401 tagtaacatc aaatagttaa aacgattgga ttcttttagg tagcataatt ttaacttaga
    14461 aaaaacagct aactttttag tttgatcggt ttctttggtt tggatctctc ctttatgatt
    14521 agaataaata aattcatcaa tcaataaaaa ttgcttgagt gatacattta tttttcggat
    14581 cagttctttg attgtccgtt ttggaatatg taagtcattg gataattctt gtaaagaaca
    14641 attatttttt tgaatgacca tttgtagaac aagtaactct ttttttcttg cttcaattag
    14701 cataaatacc tcctttttat tctgtttttg tatatagtta gacaaaaact gtgagtggaa
    14761 agtcatttca gtctgtgtta ttctcacaga gtgtcgcctt cttttttata taacgttatt
    14821 taattttttt gttaccttgt ttattctatt gtttatttcc tagggtattt tgtcaagata
    14881 ttcttgtttt taaggcggaa aaattgattt ttaatggata taactaaata tgttttgata
    14941 attatcaata atatttttag aattatttat gttatttatt tatgggtaga tgaaaagaga
    15001 cggaggtaag aatgatgggg agagtaacaa attatagtaa agaatatttg ctgttcaaaa
    15061 gtatggtata cgtggaagaa tatggaatga agtcaattac tgcaagagag ttagcagagt
    15121 tctgcggttg ttctacatac ccgatttata cacatttcaa atcaatcagc gggttaaaag
    15181 agaagatatt agaagaaatc actgtttatt ttgaaaacta cttagctgaa tgcagttccg
    15241 atgatgtttt aagcattgtc tacttattga aagatttttt ttctatccat gaaagtattc
    15301 gtgaaatcat tgcaaaatct gatttagaca tggattcgct atttaagcgg ccatttttct
    15361 catatgtcaa aggtcacacg aagatcaaag aagaaaagtc gcttgaattg gtatggctta
    15421 atgcattggg aagtttgcgc tcagatgatt cttctcaaga ttttcttgaa atgatgaacg
    15481 ctgtttggga taaaaacgag gtgacggatg aatacttgga aaatatctta aaggaacagt
    15541 caccttaggc agcaacatga aaaagtagat cagccacctt tatttttcgt aaaaaagatt
    15601 gtaaaatgtc taacgaaaca ttggaatgaa ggaggaagcg acaacgttac cacagcaatt
    15661 tatcaaaaaa tatcgtcttc ttttaggcga agaagcatcc gatttttttt ctgcgcttga
    15721 acaaggaagc gtgaaaaaag gatttcggtg gaatccacta aaaccggcag gattggacat
    15781 ggtccaaact taccatagcg aagaattaca acccgctccc tacagcaatg agggatttct
    15841 tggcactgtc aatggaaagt ctttcctcca tcaagctggg tatgaatata gccaagaacc
    15901 aagtgcaatg atcgttggaa cagctgctgc agcaaaaccc ggtgaaaaag ttttggattt
    15961 gtgtgcggct cccggtggta aatcaaccca attagctgct caaatgaagg ggaaagggtt
    16021 attagtgacg aatgagatct ttccgaaacg tgcgaagatt ttatcagaaa atatagaacg
    16081 atggggagtt tcgaacgcaa tagtgacaaa tcatgcaccc gcagaacttg tccctcattt
    16141 ttcgggcttt tttgatcgga tcgtggttga tgctccttgt tcaggagaag gaatgttccg
    16201 aaaagatcca aatgcaataa aagagtggac agaagaatct ccgctatact gtcaaaaacg
    16261 tcagcaagag attttgtctt cagcaataaa aatgctaaaa aataaaggac aactgattta
    16321 ttcgacttgt acgtttgcac cagaagaaaa tgaagagatc attagctggc ttgtagaaaa
    16381 ttatccagta acgattgagg agattccttt gactcaatcc gttagttcag gacgttctga
    16441 atggggaagc gtagctggtt tggaaaaaac aatacgtata tggcctcata aagatcaggg
    16501 tgaaggacat tttgttgcaa agctgacttt tcatggacaa aatcagatgc ataaagaaaa
    16561 gaagacacga aaaaaaagta aagtgcaaat gacaaaagag caggaaaaac tctggacgga
    16621 gttttcaaat gattttcatt atgaagctac tggaagactt ttagtgttca acgaccatct
    16681 ttgggaagtt cctgagttag ccccttctct cgatggttta aaagtcgtac gtacaggttt
    16741 gcatttagga gatttcaaaa agaatcgatt tgaacctagc tatgcattag cgcttgctac
    16801 aaaaaagata gagaatatac cttgcttgcc gatcactcaa aaggaatggc aatcttacac
    16861 agcaggagaa acatttcaac gtgatgggaa tcaaggatgg gttttgctag tattagataa
    16921 gatacctgtc ggatttggga aacaagtaaa aggaacagta aagaactttt tccctaaagg
    16981 attgagattt cactgagtgg attagttcgt ttattttttt gtctatgcta tgatggagat
    17041 agataaaaag gagctgatac ttatgaaaac atcaacaaaa gtaacaatcg gattaagcgt
    17101 agcagccgct gccagtgtag cgacagcagt cgttgtatcg ggaaaagtaa tggaaaagat
    17161 ccatcatatg actactcgga cgaaagtaaa aaaattcgtc tatgacaaat ttgatggaaa
    17221 tgaaaaatta ctagaaatcg tagatggtct ttcagatagt gacttggatt ctttgatggg
    17281 aatgttagca aaaatcaaat caggaaaaaa gaaaatctct gtttatggtg attcaatcaa
    17341 agattcaacc gaagatgtaa aagaccggct aatgcatttt gttgaaaaaa tgatgtaaga
    17401 gaaactagct tttgaacact attcattcga aataaggggt tgtgacaaaa ttattgtcac
    17461 aaccccttta atctatcttt aattatgttc atgaagcaat aaaagatagc aaaacagtaa
    17521 atcgttggct ttttttagat tgaaaccaga ctgttcttga aatttttcga ttcgatattt
    17581 tagagtatta cgatgaagaa acagtgtttt tgcagcagag ctgacattgc ctaattcatt
    17641 ccataattct tgaatgatca cttccatttc attttgtttg atcagttcta tgtaactttt
    17701 gatcaactta ctatctttta cagtatcttt tgtatagtaa tgtaaagcca catcttgcat
    17761 agaaaaagta cggctgtgag tataaagatt gttttgctct gttaaaaaaa tttttctttc
    17821 ttcagcaaac aaagggacga ggtcggtgcc aggtgaatgg aaagaaccaa cgaatacaga
    17881 cgttgtcgta tcaaaatcag catctaaaga tagaaaaata ccgttgattt cagtagcatc
    17941 aagataatca cggtttattt gttcgactaa gagcgcatct gtgtctgtat aaaaaaagaa
    18001 atcagcagaa tagcgaaata attccaaaat agtatcttgc cattcttttt ttaagaattc
    18061 tccttttgat tcgacgtgaa gctggatcaa acgaaagctt ttttcttgaa cacaagaagc
    18121 atctttaaaa agatagtgat accatgggtg ttttttttga tcgttgatta cagggaataa
    18181 cgctttcaaa aggctggttt cttgttgaga taatgaatct tgtttgatcc agataaaagc
    18241 atcatcaatt gcaaaagaaa ggatatccgc ttctgatgct ggagagtttt ttttctgtgc
    18301 ttgtgggtaa agagcaagta atttatccat tttcatcttg ttcacctctt gtatttattt
    18361 taccatagaa aaaaaagtta gttacagcct ttttatcata tgctaaagta aaacaagaaa
    18421 aaacaaaggc agggaagggt aattggtaaa tacaaatctt gatgcagtag aaaaagaaaa
    18481 atggatgcgc cttgcattgg cagaagcaaa gaaagcagaa atgcttcatg aagttccgat
    18541 tggagcagtg gttgtattgg atggaaaagt gattggaaga ggatataacc ttagagaaac
    18601 cacgcaagat gctactacac atgcagaaat gcttgcaatc aaggaagcct gtgaaaaagt
    18661 gggaagctgg cgcttggaag atgctgcact ttttgttacc ttagagcctt gtcctatgtg
    18721 cagcggcgcg atgattttgt ctcgtgtagc agaagtttat ttcggcgcgt atgatccaaa
    18781 aggcggaaca gccggtacct tgatgaatct tttggaggac gagcggttta accaccaagc
    18841 atatgtagaa ggtggcattt tagaagacga atgtgggatg attttgactg actttttccg
    18901 aaagctccgc cagcgaaaaa agaaattaaa aaaagactgg tcaacgaatg aaaaataagg
    18961 tatactattc tttgccgaaa ggctaggaca atggtgggct tgtgaagcgt gtcaggtctg
    19021 gaaggaagca gcactaagca ggttccgcca tgtgtctgat tataatgaaa cgacaacaga
    19081 ctttcgggtc tgtttttttt atttttatag tactgtaaaa atgaatgttc ctgatttttt
    19141 agccagactg atttatcgaa tattgtactt atacaatttg cttcaaggca ggaaaaactc
    19201 tgaaatagag cttgtttcaa tggaagaaaa agctgcctat ggtatgataa agataaagag
    19261 aggcgagagg gtacatggaa aaaatagtga ttttacatac gaatgatctg cattctcatt
    19321 tagaaaattg gccaaggatc agaaggtttt tggatcaacg taaacgagaa aacgaaaaga
    19381 aagaaaaaac gacaacgatt gcagtagatc taggtgattt tgtggatcgt tggcatccat
    19441 tgtcagaagc aacgaatgga caggaaaata tcttgctgat gaatgaagtt ggttatgatg
    19501 cagcgacgat aggaaacaat gaaggcgtag gaaattccaa agaagagttg aatcatttat
    19561 acgatcatgc tgagtttgat atcatcctgg ataacttatt cgataaaaat acgctccagc
    19621 caccggcatg gacgaaacca tataaaatca ttacaacgaa acaccagacg aaaatcggac
    19681 taattgcatt tactgctcca tttccgctta cttataatcc gaatggctgg gacatacgga
    19741 atccttatga tattttgcca gaattagtcg aagatttacg ttcacaagtg gatgttttag
    19801 ttttgatgag tcatctcggg attcaggatg atttccaaat tgcaaaagaa ataccagcaa
    19861 ttgatgttat tttaggatcg cacacccatc atttattcag agaaggtcac gtaatcaatg
    19921 atgtccaaat agcagccgct ggaaagtacg ggcagtatgt cggtgaagtc catttaacca
    19981 tcgatgaaga gaaaaaaatc cttaagcatt cagccaaagc catccctacc gaaacaatga
    20041 cagcttttac agaagatgaa caagaaataa atagttatct agaacgtggg catgaattat
    20101 taaaagaaaa agaagtagca gatttgccat tttctctgag tctagatatc ttcaatgagc
    20161 attcgtttat ccaagtagcc tttgaggcgg tgaaagaacg aggaaagacg gaagcagcaa
    20221 tattaaacag tggtctgttt ttaactgata ttccaaaagg gcgggtcaat caggatcagc
    20281 ttcacactgc gttgccgcat ccgatgcact tgttaaatgt cacattgggt ggaaatgatc
    20341 tgatccgctt agttttagaa atagaaaaaa atcgtaactt tttacgtaac tatcctatga
    20401 agggaatggg atttcgcggt aagatttttg ggcagatggt ctataatgga atcacatatg
    20461 attctattaa ccatcaagtc ttttggaaga acaagccaat cgatctgaat aaaaattata
    20521 cttttactac cgtagatcat ttgatgtttg ttccattttt tccaacgatt gagattgctg
    20581 gaaaaaatga atttttgttc cctgaattta tccgaagtgt ggtaggagat tacttgaaag
    20641 ctcactaccc aatcaaataa aaacaaggta taataaaaaa gataggtggt gaacgcatgt
    20701 ctgcaaaaca aaatgaacag cagaacgttg agcataaaac agaaattgct aagaaaaaat
    20761 catcagctga acaggaaaca acagtcaccg aagaactgac agctgctttg gaagaaatcg
    20821 ttgaggagcc taaagcagaa aaagtcaaag gcgagctagt gacactgtta gatgacagta
    20881 attttttgat aggtgaaaga cattatcggc ttgtgtccga ttatcgtgaa ggatttaatg
    20941 cagaaaagct tggagaacgt tacagtgatg tactagcccg atatgattat atcgtaggag
    21001 attggggtta tgaacaacta cgcctaaaag gatttttcca agcggataat cgacgggcgc
    21061 atccagatca gcgaatcgat tccttggaag actatcttta cgaatattgc aattttggtt
    21121 gtgcgtattt tgtcatcgag agaatcggcg gtaaaaaaga aaagacaact cagcgccgaa
    21181 aaaagaaaaa aaaccataat cgtgcgtata tcgatgagaa aaaaggacca gtcgcatcta
    21241 atcgcaaaaa accggttatt aaaaatcgaa aaacagagtc agcaaaaacc tctgaaaaag
    21301 taaaaaaaca agaacaacct aaacaagtag aaaaaacaaa aacagcattt acgatcagaa
    21361 aaagggaaga gtagtggatg aatggactat aaaggatatt taattgattt agacggaacg
    21421 atttatcgtg ggacacaacc cataccggcc ggtaaacgat ttgtggaaga attgcaaaaa
    21481 agaaaattac ctttcttgtt tgttaccaat aacacgacta agtcaccaga aacagtagct
    21541 aatcgtctag cagatgagtt tgacattcat gtagctccag agacagtcta cacagctact
    21601 ttggcaacaa tcgattttat gaaagcagat ggaaaaggga aaaaagtcta tgttatcgga
    21661 gaagcaggat tgattgactt gattttagct gccggattta catgggaaga agaggctcct
    21721 gactatgtag tggtaggctt agataattat ctgacctatg aaaaagtagt taaagcaaca
    21781 cttgctattc aaaaaggtgc tactttcatt ggaacgaacc cagataagaa tatacctact
    21841 gaacgcggcc ttcttcctgg agcagggtca gtcatctctt ttgtagagac agcaacccaa
    21901 acgccgccga tctacatcgg aaaaccagaa gccatcatta tggataaagc cgttgaagta
    21961 ttagggctgc aaaaagaaga agtaatcatg gttggagata actatgaaac agatatccaa
    22021 gctgggatac gcaataatat agatactttg ctcgttttat caggttttac aaaagaagaa
    22081 gatgtacctg ggttgcctat cccagctacc tatgtgaaac aatcactgga tgaatggagc
    22141 ttctaatatg aaaaattcga gatggcagtg gatggaatat gctggattgt tcagcctatt
    22201 tctgacactg atcagtctgg cagttggtgt gacgatcaac tttcgaccac tatacgtatt
    22261 tgatatcgga catttgcaaa ttttggatta tacttcttta gatcaagaga cgttactgaa
    22321 aaattttgat cacctaatga attatctcaa taatcccttc aaaaccatcc tcagcttgcc
    22381 agatttccca gtttctgcta gcggagccca tcacttttat gaagtgaaaa ttttgttttt
    22441 agtggattat gctgtgtttt tcatcacttt gatcccgagt attttgttta tcaaatattt
    22501 gcaaaagaat gatcggcttt ggcggttgat tcgtccgttt caaatcggca tgttactgcc
    22561 agttgttttc ggttttttca tgatgatagg atttgatcgc ttttttatcc tttttcatga
    22621 aacgtttttc aacaacgatg actggttatt cgatcccgtt actgatccaa tcatcaatgt
    22681 acttccagaa gaatttttca tgcatagctt tatcttattt tttgtattat tagaattatt
    22741 ttttgctgtt tttcttttct taggaaaaaa tagcttgaaa caaacaaaaa agaaagaact
    22801 cgtctaaata gacgttttct ttcttttttt gttgaattat tgagtaaggc gacttctttt
    22861 ttctggttct ttgtgtgcta atcgactggc agcagcagcg gcgatcgctc cgacgatatc
    22921 atctaagaag gtatggacat gaacgccatc gtggctgttt agttctttta aaataccagg
    22981 tttcaccttg tcgatataac cataattcgt aaagccgatc gtaccgtaaa cattcacgat
    23041 tgacaaggct aggatctcat cgataccgta aaggccttcg tcatcttcga taatatgttg
    23101 caacggactc atcaattttt tcttttctgc taatatatct aattggattc cagtgataat
    23161 ggcgttatgt acttcacgct ttgttagcac gctgttgaca ctttcagtac aatccgccaa
    23221 agtcaaatca gaaatatagt ctttttgtaa aaacatgaca agttgtgcga tatcttctac
    23281 agttacgcca cgttccttca atagttctct tgccttttct tctaaagtct cagttttgac
    23341 gaccatatta ttgctccccc tttatttttt ctttctctat ttcatgttaa cggaacatga
    23401 aaaaaatgca agaaactgct ttcgggttta acttgaaatg caaagagata taaaaaatca
    23461 actaggacga agatttttat cgtataagct tgtgctgtga ccaggctgag ttcgttcctt
    23521 tggattaata tagtgtaaag cgttgttcac tgcagtaggt gcttcaccga gcccagtagc
    23581 aatcagtttc acttttcctt cataggtaca gatgtcacca gctgcataaa cgcctggaat
    23641 agaagtagac atatctgatt ttacagtaat ggcatttctg gtactatcaa gtccccaaga
    23701 agaaagatgt tctaaattag aagagaatcc ataattgacg atcagagaat caatcatcaa
    23761 atcaatcgtc tcgtcacttt tcactttttt taaccggata tctgtcagtt ccccattatt
    23821 gcctgataat ccatcgatga gatagggagt aagcaagttg acagatgaag atttcaagcg
    23881 tgatacacta tgttcgtgcg cacgaaattc cgggcgacga tgaatcaagt aaacttcgct
    23941 tgcaataggc tctaacatca acgtccaatc gatagcagaa tcaccacctc cagcaatagc
    24001 gacttttttt ccagcatact tcatcaaatc attcacgaaa taatccaatc cgtggttttc
    24061 aaatgattca gcattatcta aattcaattt tcttggctgg aatgaaccat ttcctaaagc
    24121 taaaatgact gctttggaat aatggatgcc tttatttgta gtaatctcga taacttcgtc
    24181 ttcacgagta agcgagagaa cttcttcttt taaatggaat gtatggttaa aagtagttaa
    24241 ttgtttttct aattgatcga ttaattcact tgcttttatt gctggatacc caggaatatc
    24301 gtaaatatat ttttcgggat acaaagttgc taattgacca ccaagttgag gtagactgtc
    24361 aatgattttt gttttagctt ggcgcattcc tgcgtaaaaa gcggcaaaca tccctacagg
    24421 acctgcaccg atgattgtaa tgtcatagat ttccataaaa attcctccct tttcacacta
    24481 tagcagaatt taattgagaa tgcttctcaa aaatgtttga attcgaaatc gaaacggtaa
    24541 gttatagaat gtatctagtg atgaaaaata aaagaaaaca aatctagtat ttttacgaga
    24601 ggttaggtat tatgttatta ttattatgaa gtatgcaaat attaggagga aacaatatgg
    24661 cattcccaca actagattta aaaagtgaaa aaggaccaaa agctgttatc aaaacaaacc
    24721 gtggtgacat tactgttcaa ttattccctg aactagcacc gaagacggtt caaaatttca
    24781 ttgaactttc caaaaaaggt tattatgatg gcgtgatttt tcaccgcgtt attcctgatt
    24841 tcatgatcca aggcggcgat ccaactggaa caggaatggg tggcgaaagt atttatggcg
    24901 aaagttttga agatgaattc agtcgtgaac tgttcaacct tcgcggagct ttatctatgg
    24961 caaattcagg tccaaacaca aacggaagcc aattctttat tgtaaataac acaaatgtgc
    25021 cagctaatat gcttggacaa ctagaaggtg ctggattccc atcagaaatc attgaagcgt
    25081 ataaaggtgg cggaactcct tggctagatt tccgacatac agtctttgga catgtactag
    25141 aagggatgga cacagtagat gaaatcgcaa atgtacaacg tggtccacaa gaccgacctg
    25201 tacatgatgt agtgattgaa acaattgaaa tttcagaata gtttggtatg gtacggagta
    25261 attatgaaaa aggacgaaac ctagtaaggt ctcgtccttt tattttgaat aaatgattta
    25321 atcgattgtc tctaaagcat acttgattcg attaagtcct tccagtaaag tttcttctgg
    25381 acaagcaata ttcaagcgca tatgcccatg accggaagga ccaaacgtga tgccaggatt
    25441 taatacgact tttcctttcc gaacaagagt gttctccaat tcttggtcgt ctgaaatata
    25501 ggcggtaaaa tctaaccaca ttaagtaagt tccttcaggg ttcattattc tcactttcgg
    25561 aagatactgt tcaaaaaact gattaactgt ttgaacattt ttttctagat aaggaatcag
    25621 ttggcttaac cattctccac ccgtctcata agccgcttgg gttccaatca gtccaaaagt
    25681 gttgatttcg tgctgttggt tcatcactaa atgtttttca aaatcagctt tcatctctgg
    25741 atttttgata aataccattg aatttttgat tgcagctaga ttgaaagtct tggttgcgga
    25801 agtaaaagca actagtagat catctaaatt aggatcaatc gttagcatgg aagtaaatgt
    25861 atgatcgaat aaagttaaat cttgatggat ctcgtcacta aacagaatca cttgatgttt
    25921 taaacacaat tcgcttaact gtttcaattc ttctttgctc caaacacgcc ctccaggatt
    25981 gtgaggattg cataaaatca ttgcttttac attgttttct tcgatttttt tctccatgtc
    26041 agacaagtcc atgatgaaat gattattttt ttctagcaaa gagcttcgaa cgatgtgacg
    26101 ttgatttgtt tctataatag aggaaaatgg aggataaact gggtcatgaa tcaaaataga
    26161 atcgcctggt tttgtaaaag tttgtactgc tgcagctaaa ctggttaaca caccacttgt
    26221 aaaaacgata ttttctttcg tcaactccac agcatggtgt tggcgttccc aatgaatgat
    26281 agcctgatat agtttatcag acagagtggc ataaccaaag attccatgtt tgatgtattc
    26341 actaaacgct ttagctaccc caccgggaga caagaagtcc atatctgcta cccacaaagg
    26401 aagtaagtca tccatttgat atgtctcagc aattgcgtcc cattttacac tttctgtatc
    26461 ttttcttgaa atctgtttat caaattcgat catcttaaga cctccagtca ttttcgtcgc
    26521 ttcttttttt gagctattta cagtataatg ataaatgcga agagagggaa gtagatgact
    26581 tataaaattg gacaaataat tgaaggaaaa gtgacaggga tacagcctta tggcgcattc
    26641 gtttctcttg ataacgagac gcaaggactt attcatgttt cagaagtaca atccggttat
    26701 acgaaaaata tccattcatt gttaaaagtc ggacagccag taacagttca gatcatcgac
    26761 attgatgaat attccaaaaa aatcagttta tctttaagaa cgttagaaaa aacaaatcca
    26821 gctgttccat atcgcagaaa aaggtatttc acgaataaaa atcgtaagat cggatttacc
    26881 actattgcgg agcagttacc tatttggata gaagaagcat tatctgaact agaacggaaa
    26941 gaaaataaga aataaaaagt gtattttttc tgaaaatgtg ctagaatttt tatgtaagcc
    27001 atagaacaag ttgatcgagg tggaaatttt ggcaagcaaa agagttgctg gtacaattat
    27061 gttgcatttg gaggatggta ctaaaaaatt tttggtccat tcaattgatg acaagttgga
    27121 atttgcgtta gcggaacttt ctgaaggaaa gactggttta gcgaatatac ttaatttctt
    27181 aaaagaaata gttcatatcg atgttagcaa gattagtttg atggaattga caaatacaca
    27241 tattaaagag gaaaatgttc cgttgtttgt ctttgaaact gaacaaaaga atcaaagaga
    27301 agaattaggc gaaggatata tttgggaaga accaggtcaa atgcgctctg ttcttggaac
    27361 ttacgaagta gagggagtgc ctttcttcta aaatacagat taagcgaaca aagaagtttg
    27421 aatttttttc aaacttcttt ttttattgtt tggtccaaaa aatacaatga aatgttctgc
    27481 taaagatagt tttttagtgc attttatttt caaaatatgt tgaaaacggt tgtataacga
    27541 ataataataa ttgatcgaaa aatcatttca caaagtaatg cttaatttat agttgaccaa
    27601 ggaagaaata catgataaat ttaacagcgt tgattctgaa gtggatgtta tatcacattt
    27661 ctaaaaaatt taaaaaa
//
LOCUS       DACEUF010000034        26582 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.035, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000034 DACEUF010000000
VERSION     DACEUF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.035"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(99..1229)
                     /locus_tag="IXG20_001386"
     CDS             complement(99..1229)
                     /locus_tag="IXG20_001386"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ1609591.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDRLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IXG20_001387"
     CDS             complement(1878..3080)
                     /locus_tag="IXG20_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1609592.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IXG20_001388"
     CDS             complement(3140..3343)
                     /locus_tag="IXG20_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1609593.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="IXG20_001389"
     CDS             complement(3485..4552)
                     /locus_tag="IXG20_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1609594.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            4879..5691
                     /locus_tag="IXG20_001390"
     CDS             4879..5691
                     /locus_tag="IXG20_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1609595.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6867)
                     /locus_tag="IXG20_001391"
     CDS             complement(6715..6867)
                     /locus_tag="IXG20_001391"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609596.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7058..7936)
                     /locus_tag="IXG20_001392"
     CDS             complement(7058..7936)
                     /locus_tag="IXG20_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1609597.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7975..9093)
                     /locus_tag="IXG20_001393"
     CDS             complement(7975..9093)
                     /locus_tag="IXG20_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ1609598.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9090..11189)
                     /locus_tag="IXG20_001394"
     CDS             complement(9090..11189)
                     /locus_tag="IXG20_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ1609599.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11240..12061)
                     /locus_tag="IXG20_001395"
     CDS             complement(11240..12061)
                     /locus_tag="IXG20_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1609600.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12058..12867)
                     /locus_tag="IXG20_001396"
     CDS             complement(12058..12867)
                     /locus_tag="IXG20_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1609601.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12888..13382)
                     /locus_tag="IXG20_001397"
     CDS             complement(12888..13382)
                     /locus_tag="IXG20_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1609602.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13394..13882)
                     /locus_tag="IXG20_001398"
     CDS             complement(13394..13882)
                     /locus_tag="IXG20_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1609603.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13959..16655)
                     /locus_tag="IXG20_001399"
     CDS             complement(13959..16655)
                     /locus_tag="IXG20_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1609604.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17083..18156)
                     /locus_tag="IXG20_001400"
     CDS             complement(17083..18156)
                     /locus_tag="IXG20_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1609605.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18153..18980)
                     /locus_tag="IXG20_001401"
     CDS             complement(18153..18980)
                     /locus_tag="IXG20_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1609606.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18981..19787)
                     /locus_tag="IXG20_001402"
     CDS             complement(18981..19787)
                     /locus_tag="IXG20_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1609607.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19799..20884)
                     /locus_tag="IXG20_001403"
     CDS             complement(19799..20884)
                     /locus_tag="IXG20_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609608.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20901..21443)
                     /locus_tag="IXG20_001404"
     CDS             complement(20901..21443)
                     /locus_tag="IXG20_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1609609.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21701..22690
                     /locus_tag="IXG20_001405"
     CDS             21701..22690
                     /locus_tag="IXG20_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1609610.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22687..23424
                     /locus_tag="IXG20_001406"
     CDS             22687..23424
                     /locus_tag="IXG20_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ1609611.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23421..23972
                     /gene="coaC"
                     /locus_tag="IXG20_001407"
     CDS             23421..23972
                     /gene="coaC"
                     /locus_tag="IXG20_001407"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ1609612.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23988..24620
                     /locus_tag="IXG20_001408"
     CDS             23988..24620
                     /locus_tag="IXG20_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1609613.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24705..25352
                     /locus_tag="IXG20_001409"
     CDS             24705..25352
                     /locus_tag="IXG20_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1609614.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25357..26340
                     /locus_tag="IXG20_001410"
     CDS             25357..26340
                     /locus_tag="IXG20_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ1609615.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8130 a         5615 c         4508 g         8329 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ctatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat
     7201 cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat
     7261 ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct
     7321 gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg
     7381 ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat
     7441 tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca
     7501 cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa
     7561 cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat
     7621 ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat
     7681 tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa
     7741 tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa
     7801 aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt
     7861 ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa
     7921 ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg
     7981 cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata
     8041 ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat catcgttgcc
     8101 gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt gaagatgcca
     8161 acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt
     8221 tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat accctaaaaa
     8281 ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat gaaaggccca
     8341 cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact cactatcccc
     8401 atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc
     8461 aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact tataatcctc
     8521 ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc tattttcttc
     8581 caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat ccttcggatg
     8641 taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat aggaattaaa
     8701 gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc
     8761 attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc catcccaata
     8821 aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt
     8881 catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag tttcttcacc
     8941 agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc ttggcaacat
     9001 cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac tctggatttt
     9061 tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat gtgaatcgtt
     9121 tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg cttttctgac
     9181 aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac
     9241 ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa tactcctttt
     9301 ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc cgatgcccag
     9361 ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc taagcgatac
     9421 ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc taatcgggtt
     9481 acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc aggaaaagtg
     9541 gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt tggattgaaa
     9601 gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc taaaaattga
     9661 taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa attaattttt
     9721 ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg tttttgatag
     9781 tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa ttgctcacca
     9841 aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca ggcaatgttt
     9901 ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc aagtccaata
     9961 caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc aaaggcgtct
    10021 gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc taatatgtcc
    10081 tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc
    10141 ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt actcagagaa
    10201 tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg atcaaaccat
    10261 agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga tgcgtaatac
    10321 atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct gccgacgacc
    10381 tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg acgatagaga
    10441 tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa atagttcaat
    10501 gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc ttcaaaagca
    10561 tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta
    10621 tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg atacgaaggc
    10681 acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta aacctgttga
    10741 acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac aatgacctta
    10801 aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc gacttcaata
    10861 cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt taaggataac
    10921 gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc agttagtaaa
    10981 ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc
    11041 ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg atagtagctg
    11101 tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa tgggtgtttg
    11161 ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc
    11221 tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt cccaagacga
    11281 ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg aaacagctca
    11341 atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct tggacattca
    11401 ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc atactgatcg
    11461 tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga atttcaatta
    11521 acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct aaaacgccgc
    11581 catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata attggtgcca
    11641 aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc
    11701 cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt ttgatcgcgt
    11761 tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc gatgccgcaa
    11821 tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca
    11881 gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc atcatagaat
    11941 aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata gtcatcgagc
    12001 gccaaaacat cttccgaacc aattttttct catctttaga aattccatca gctaatttca
    12061 ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat cgctttgaac
    12121 tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa ttgcaataat
    12181 cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa caacggcccc
    12241 aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca tcgccatccc
    12301 aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct ctggaatatt
    12361 atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg gaataagagc
    12421 ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc ctttttgatc
    12481 accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa aagaaaaaat
    12541 catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa taatcgcatc
    12601 cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc
    12661 cgccgtacta gagattccga tattgacgac acccataaag atggcttcta gtgacgcccc
    12721 gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag caacaacaat
    12781 cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa taaaaacggc
    12841 caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta aaaatcactc
    12901 aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac ggtatctgga
    12961 taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat cacttccttt
    13021 gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc cacgttcatt
    13081 aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac attagggact
    13141 aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat cgcgtcatca
    13201 actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt catcttttga
    13261 atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt tagactcttt
    13321 gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac tgctttaatc
    13381 atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc tcatcaagaa
    13441 tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc tcctttgtta
    13501 attccttggt tgccaatact atttcgagca gaattggcag attaaagcca gacaaaatag
    13561 ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca cttccgccaa
    13621 ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca aatacattca
    13681 actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc tcttgaacct
    13741 tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta
    13801 ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata taagcaattc
    13861 ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa aaaagtgtct
    13921 aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct
    13981 aataattcat agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc
    14041 attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca
    14101 taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc
    14161 gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca
    14221 tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa
    14281 cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat
    14341 tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct
    14401 aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg
    14461 atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt
    14521 tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca
    14581 tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt
    14641 tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca
    14701 atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc
    14761 gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt
    14821 tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc
    14881 acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac
    14941 gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta
    15001 ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat
    15061 aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa
    15121 tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga
    15181 tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt
    15241 tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc
    15301 tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg
    15361 tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct
    15421 tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga
    15481 tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact
    15541 tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct
    15601 tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca
    15661 atattcccac taaagggatg agataacatt aaagacaagg cttctcttga aacaataata
    15721 tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct
    15781 cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag
    15841 gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca
    15901 gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag
    15961 agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg
    16021 ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg
    16081 tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag
    16141 atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt
    16201 gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta
    16261 ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga
    16321 ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca
    16381 tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact
    16441 aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc
    16501 tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct
    16561 ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc
    16621 ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc
    16681 gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat
    16741 atgattttta tgttttttca tagctacaaa tattgaactg gattcatata ttagttttat
    16801 taatagatta cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt
    16861 aactttttca atcttagatt tactagcccg tctcgggccc tattttacaa tggttttgtc
    16921 cacttttctc tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt
    16981 ttcatccgct tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc
    17041 tttagaacgt tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt
    17101 gaattctaag aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac
    17161 ttcaagatgg gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt
    17221 tggcaataac gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg
    17281 tgccgcattt tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt
    17341 cacggttttt ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac
    17401 ataatgtaaa tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc
    17461 cacggaggct tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac
    17521 atttggtgtt aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact
    17581 attcaaagaa tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat
    17641 cagcatcaaa ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt
    17701 tgcatcaaag aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga
    17761 atactgatta tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc
    17821 tttgatcttc gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat
    17881 atattcactt ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat
    17941 cgcttcatta gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt
    18001 cgtgatcaaa ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc
    18061 actcatgcca cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat
    18121 gagaatacct aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt
    18181 actcttctgt ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca
    18241 atgaccaata tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg
    18301 attccttgtc ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg
    18361 acgaaaaatg taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct
    18421 atgattcctg gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca
    18481 ccaaggtctc tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc
    18541 atcaagacga cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct
    18601 aatccgaatc ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga
    18661 gagactaata agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg
    18721 taatagattc ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa
    18781 gccagcatga acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca
    18841 taattttccc aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa
    18901 aaaatcagat agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac
    18961 gaccatttaa attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc
    19021 atcacgatga acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt
    19081 tgtgtaacca agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca
    19141 atcagacgag tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt
    19201 ttcacaccat ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg
    19261 cctaaatctc tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt
    19321 ggcataatca taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg
    19381 aataatatct gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc
    19441 acactgaaaa tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa
    19501 ataatcagca tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg
    19561 taactgatga tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc
    19621 atagaaagcg atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca
    19681 ttcatatcaa aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg
    19741 agccacaaaa tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct
    19801 attcttcgta gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga
    19861 catggatatc ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg
    19921 ttgaatggac catccattca ttttgctgtt catcataaca gatgatttca taatggacac
    19981 cacgaaacag ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg
    20041 tcaaatcctc tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga
    20101 cacattcaaa ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca
    20161 cgatattgct ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat
    20221 ctacgggtgt accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca
    20281 ttgccaacgc ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt
    20341 gcagatcccg cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg
    20401 gttcatctaa tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct
    20461 gtctttgacc accagacatt tcactaatct cccgtttttc gtatccagga agctggacca
    20521 tacgcaaagc atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca
    20581 atccaaaagc aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga
    20641 ctgtatttac ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga
    20701 tgtccccttc tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac
    20761 agccggaagg accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt
    20821 ttaatactgt ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc
    20881 ccacgttttt accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt
    20941 cgttttttga gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt
    21001 cctgcttttg ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca
    21061 aaaataaacg tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa
    21121 gtcagcaaga caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca
    21181 tacccgtttt cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga
    21241 ttcgctgctt cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa
    21301 aagaatgttt ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt
    21361 gacaaatctg tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt
    21421 cttaacttct caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt
    21481 cctgtttact accactcgta ttagtatacg aaatttattg aaaaaggcaa ccctttgtaa
    21541 aatttttttt gctgaatatt tctttatctt ttggaataaa ttctagttgt ttgattcttt
    21601 atttttcacc taatttttgt ctaaatttac ggaagaacaa gaataaaaga gtaggcttta
    21661 tggtatgata aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt ttcgatcttt
    21721 tcaggtaata aaggaacctg ttttccatac agcaatcagt gatctgccat ttactgagtt
    21781 ggctgaaaat gaagtcttgg taaaaattgc ctattctgat gtcaattata aagatgcatt
    21841 ggcaatgagc gagtctggtc aggtgatacg aacatatccg atgactccag ggattgattt
    21901 atccggaact gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc ttgtacttgc
    21961 tactggtttt gggcttggcg tcacccatcc aggcggttat agccaatatc aaaaagtacc
    22021 aggagactgg ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga tgatccttgg
    22081 tacagcaggg tttactgcta tgttgtgtgt gaacgcttta atccgtcaag gtatgacctc
    22141 cgataaaaaa gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag cgatagccct
    22201 gttacataaa ctaggattcc agtcaatcat tgccttttcc agaaaagaag agtctgtcac
    22261 atggctaaaa agtctaggtg ctttgcaagt cgtacgacct gatgaatttc tgccagagac
    22321 aacaaaagct ttaggaaaac agcaaatcga ctatgtcatc gacacagtag gtggcgagca
    22381 gctaagcagt cttcttccat tgatctctta taacggtgca gtagctttat gtggcaatgc
    22441 tgggggaatc aaactaaacg cgactgtctt gccttttatt ctgcgaaaca tccagttgat
    22501 tggaattgat tctgtcaatg taccaataga tcaacgtctt tctttatggc aacagatggc
    22561 ggatttgcag attgcagatg aactggtcgt ccaagagatt actctggatc aattgcctga
    22621 aacagcatct aaactacttg ctggaacaca ccaaggaaga acactagtaa atgtaggtga
    22681 ccataaatga agattctaat cacagctgga ggaacgactg aaaaaatcga tcaagtacgt
    22741 gctattacaa atcattcgac aggacgatta gggcaagcat tagctgatta tttagctgcc
    22801 aatccggata ccacaatcga ttacgtgact acgaaacaag cactgaaacc gaaaagacac
    22861 tccaatataa cgatttatac gatcgaatct gcactggact tatttttaca attagaagaa
    22921 ctaacgaaaa aagaacacta cgatgcgatt atccacagta tggcagtcag tgattttact
    22981 cctgctttta gtttttcaga agaacagctg gcaaaaaacc tacccacttc ttcaacgcaa
    23041 gaagaactgg acaattggtt tgcggaaaat gaacagacag ataccactgt ttcaaagatc
    23101 tcttctaaca ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat catttcttat
    23161 ctacgtgaat ggcagccaaa agcaaaaatc attggtttca aactattagt agatgtacca
    23221 aaggaatctc ttctggcagt cgcaaaaaat agtctgataa ataacaaaac tgattttatt
    23281 ttcgcaaatg atttgacgga aatccatggc gagacacacc acggctatct cttatctaaa
    23341 gatggaacag tggaagaagc tcaaagtaaa tctgagattg ctgcattgat tactgagaaa
    23401 attagactgg aggaatcaaa atgaaaaaga tcttactagg cgttactgga agtatttctg
    23461 cttacaaaag cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag gtagatgtga
    23521 ttatgtctaa aagcagcaca cattttatca cgccattaac cttgcaatct ctgtcgaagc
    23581 gcgcagtcca tacagacgtc atgcaagaat caaatccgca ggtaatcaat catatcgaac
    23641 tagcaaaaca agctgacttg tttctcatcg cgcctgctac agcaaacatt atcggcaagc
    23701 tagcaaacgg cattgcagac gatcttcttt caacagtcgc aatggcgctt ccagtgggaa
    23761 caccaaaatt gattgcccct gccatgaaca cgaatatgta tcaaaacccg atcactcagc
    23821 gtaatctgga aacattgaga tctgtcggct atcaggaaat tgaaccaaga gaatctcttt
    23881 tagcttgtgg agattttgga aaaggtgcct tagctgatat tcagacgatc atcgaacagg
    23941 tcatgctgac tcttaatgaa agaaatgctt agaaaaaagg agtacttatg aaaaatacaa
    24001 aaaactttac attaactgcg atgtttttag ctattttgat tttattagct gttacgcctt
    24061 taggttttat ccctatcggt ccaatcaatg caacaaccat gcatattcct gtaatcattg
    24121 cttcgatcgt tttaggacca agactaggtg cttttcttgg cggtacattt ggactgatca
    24181 gcatgatccg tagcacagtt atccaaacac ctttgtcttt tgtcttttct ccgtttatcc
    24241 cagtcatcgg aacagaacat ggaagctgga aagccttgct gatcgcgttt attcctcgca
    24301 tcttgattgg ggtggttcct tatttcgttt ataaaggtct gcgcaaacta atgaaacaaa
    24361 aagcagattc cgtttcttta ttcttagctg gactttccgg ttcattgacc aatacgattt
    24421 tagtgatgaa tatgatctac tttttattcc agcaagatta tgccaaagtc atcggaacaa
    24481 atttaaatgc tgtttattca gctattctag cggttatttt tacaagtggt atacctgaag
    24541 gaatcgtcgc aggtcttgcg actgcagcag tatgcaatgt cttgttaaga tacttcaagc
    24601 acccttctct tactaactga tagtcatcgt agccgatctc tgattttttt ctcttttggt
    24661 gttactattg aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa atttttgttg
    24721 taggtgcaaa cggacaaatt ggccgacacc tgataaaaga tttagctcca agttcccatg
    24781 aaatttttgc aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa gagaatgttt
    24841 cgtatgtctc ttttgattta acttggtccg tggaaaaaat gaccgaagca ttcaaaggca
    24901 tcgatgtatt gatttttgct gccggctctc aaggaaagaa tcttctgcaa gtagacttgg
    24961 atggcgcaat caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc tatctgatgg
    25021 tcagtgctgt ttatgcagat gaaccagcaa aatggcctga gtccatgaca gattattata
    25081 ttactaaaca ttatgcagat gaatggctga aacgaactaa cttagatttt gtcattcttc
    25141 aaccagtcac attgactaac gatgaagaag ttacttccat tcaattaact aagcctaatg
    25201 agaaggcatc aaaaacgatc actcgctcta cagttgctgc tgtacttgcc gctctagtgg
    25261 aagaaacgga tatttctcgt acgactctag tactttctga aggtagcaaa gagttgaata
    25321 cagcattcca agaatgggca aaggaggaat agatccatgc gtgccatttc aatcaagcat
    25381 cctggttctg tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc aaaacaaggc
    25441 gaattactga tcaaagtcca tacagcagct gtcaatcgaa cagatattat gaaaagagaa
    25501 tcagtctctt tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg agaggtagtt
    25561 gagaataaaa gtgatatctc cacttttact cctgggacaa aagtatacgg attagtcaat
    25621 ctaggtggtt atgccgaata tgctgtgatg cctgccgatc gtgcaatcct tctccctgat
    25681 acgctagact atgtttctgc tgctggaatc tcagaagttt ttttgactgc ttatcaaaca
    25741 ctttactggc ttggaaaatt acaagaaggc gaaactgtct tgatccatgc aggagcaagt
    25801 ggtgtgggaa cagcagcgat ccaattagct agacagcttt ctcacgcgaa aatcatcgtt
    25861 actgcaggat cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga tgaattgatc
    25921 aactataaaa aacaagactt tcaagaagag gtacgaaaaa tcactgaagg tcggggagtc
    25981 gatgttattt tggatttcat cggcgcttct tactgggaaa agaatttagc ttctatagcc
    26041 gttgatggac gttgggtctt gattggtatg ctaggtggga ccatcgtgcc tgagatagac
    26101 cttggtgtat tgattgggaa acgtgtccaa ctgcttggta cattattgac acctagaagt
    26161 gatcagtata aagcagagct cgcacaaact tttatgcaaa aagctgggcc ttttctagaa
    26221 aatggcaaga ttcggccaat cattgaccgt acattccaat tcagcgcagt caaacaagcg
    26281 catgaatata tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat aactgaataa
    26341 aatcatgctg atcaaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
    26401 aattcacgaa aattgctttt caaatttttg tgaatttcag cttatttccg aaggaatttc
    26461 ttctgcttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt
    26521 cccacgctct ttttccgaat aaacggtgtt caaaagcttg catctgcggt aataagtcca
    26581 ac
//
LOCUS       DACEUF010000035        26527 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.036, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000035 DACEUF010000000
VERSION     DACEUF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.036"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            9..851
                     /locus_tag="IXG20_001411"
     CDS             9..851
                     /locus_tag="IXG20_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1609616.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            848..1798
                     /locus_tag="IXG20_001412"
     CDS             848..1798
                     /locus_tag="IXG20_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1609617.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1813..2046
                     /locus_tag="IXG20_001413"
     CDS             1813..2046
                     /locus_tag="IXG20_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ1609618.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            2377..2961
                     /locus_tag="IXG20_001414"
     CDS             2377..2961
                     /locus_tag="IXG20_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1609619.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3092..3760
                     /locus_tag="IXG20_001415"
     CDS             3092..3760
                     /locus_tag="IXG20_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1609620.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            3762..5186
                     /locus_tag="IXG20_001416"
     CDS             3762..5186
                     /locus_tag="IXG20_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1609621.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            5204..5773
                     /locus_tag="IXG20_001417"
     CDS             5204..5773
                     /locus_tag="IXG20_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ1609622.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5883..7637
                     /locus_tag="IXG20_001418"
     CDS             5883..7637
                     /locus_tag="IXG20_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609623.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7591..9363
                     /locus_tag="IXG20_001419"
     CDS             7591..9363
                     /locus_tag="IXG20_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609624.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            9886..10155
                     /gene="rpsN"
                     /locus_tag="IXG20_001420"
     CDS             9886..10155
                     /gene="rpsN"
                     /locus_tag="IXG20_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1609625.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            10170..10349
                     /gene="rpmF"
                     /locus_tag="IXG20_001421"
     CDS             10170..10349
                     /gene="rpmF"
                     /locus_tag="IXG20_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1609626.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            10381..10758
                     /locus_tag="IXG20_001422"
     CDS             10381..10758
                     /locus_tag="IXG20_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609627.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            10779..10928
                     /gene="rpmG"
                     /locus_tag="IXG20_001423"
     CDS             10779..10928
                     /gene="rpmG"
                     /locus_tag="IXG20_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1609628.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            10952..11935
                     /locus_tag="IXG20_001424"
     CDS             10952..11935
                     /locus_tag="IXG20_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1609629.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            11965..12087
                     /locus_tag="IXG20_001425"
     CDS             11965..12087
                     /locus_tag="IXG20_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1609630.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            12105..13628
                     /locus_tag="IXG20_001426"
     CDS             12105..13628
                     /locus_tag="IXG20_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1609631.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            13652..13891
                     /locus_tag="IXG20_001427"
     CDS             13652..13891
                     /locus_tag="IXG20_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609632.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(14084..14545)
                     /locus_tag="IXG20_001428"
     CDS             complement(14084..14545)
                     /locus_tag="IXG20_001428"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1609633.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(14740..14958)
                     /locus_tag="IXG20_001429"
     CDS             complement(14740..14958)
                     /locus_tag="IXG20_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1609634.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            15076..16692
                     /locus_tag="IXG20_001430"
     CDS             15076..16692
                     /locus_tag="IXG20_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1609635.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(16933..18273)
                     /locus_tag="IXG20_001431"
                     /pseudo
     CDS             complement(16933..18273)
                     /locus_tag="IXG20_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(18549..18704)
                     /locus_tag="IXG20_001432"
     CDS             complement(18549..18704)
                     /locus_tag="IXG20_001432"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1609636.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(19000..19332)
                     /locus_tag="IXG20_001433"
     CDS             complement(19000..19332)
                     /locus_tag="IXG20_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1609637.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(19846..20037)
                     /locus_tag="IXG20_001434"
     CDS             complement(19846..20037)
                     /locus_tag="IXG20_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1609638.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(20131..20517)
                     /locus_tag="IXG20_001435"
     CDS             complement(20131..20517)
                     /locus_tag="IXG20_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1609639.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            21211..22416
                     /locus_tag="IXG20_001436"
     CDS             21211..22416
                     /locus_tag="IXG20_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ1609640.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            22433..22867
                     /locus_tag="IXG20_001437"
     CDS             22433..22867
                     /locus_tag="IXG20_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609641.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            23484..>26527
                     /locus_tag="IXG20_001438"
     CDS             23484..>26527
                     /locus_tag="IXG20_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297336.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HAQ1609642.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVT
                     FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVS
                     AAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT         9185 a         3815 c         4995 g         8532 t
ORIGIN      
        1 ggaataatat gcttcaaaat tttattaatg gtctgtatga ctttcacttt ttacagaatg
       61 ctctaattac atctgtagtt atagggattg tttctggaac agtaggttgt tttattattt
      121 taagaggaat gtctcttatg ggtgatgcta tttctcatgc tgttttgcca ggagtagcaa
      181 tttcttatat tttaggtatt aattttttta ttggtgcgat tgtatttggt ctattagctt
      241 ctatcattat tacttttata aaaagcaata gtgtaataaa aggagataca gcaattggta
      301 ttactttcag ttctttttta gcattaggag ttatccttat aggagttgca aatagctcaa
      361 cggatttatt tcatatctta tttggaaata ttttagctgt acaagatatt gataagtgga
      421 ttacaattgg cgtttctgtt ttagtgttgg ttacagttgt tctatttttt aaagaattat
      481 taatcacttc ttttgatcca ttaatggcaa aagcaatggg gatgaatgtc agtttttatc
      541 attatctatt aatgattcta ttaactttag tagcagtaac agctatgcaa agtgtgggaa
      601 ctatattaat tgttgcaatg ttaattacac cagctgctac agcatattta tatgcaaaca
      661 gcttaaaaac tatgatattt atttcggcga cagtcggagc gtgctcatca ttgctaggtt
      721 tgttcattgg ttatagtttt aacatagcag caggatcaag tattgtttta acagcggcat
      781 tcttatttgt aattagtttt ctaatctcac caaaacaaaa atttttaaga aagaaggaga
      841 gaaagttatg atgagaaaat ggaaagcagt actgggaagt ctggggattt tgattgctct
      901 ttttatattc ggggcatgtt caacaaatag taaagacaaa gatacagtgg cttcgaacga
      961 aaaattaaag gtagtagtta ctaattcgat tttagcagat attactgaaa atatagcaaa
     1021 agataaaatt gatttacaca gtatcgtacc tattgggaaa gatccccacg aatatgaacc
     1081 tttgcctgaa gatgttcaaa aaacttcaaa agcagatttg attttttata acggtgttaa
     1141 cttggaaact ggaggaaatg cttggtttac aaaattagta aaaaatgcga acaaagagga
     1201 aaacaaagac tattttgcag caagtgatgg catagatgtt atttacttag agggtcagag
     1261 tgagaaaggg aaggaagatc cccatgcttg gttaaattta gaaaacggta ttatttacgc
     1321 taaaaatatt gaaaaacagt tagcggaaaa agatcctgat aataaaaaat tctataaaga
     1381 aaatctagat aagtatattg aaaaattgga ttctctagac aaagaagcta aatctaaatt
     1441 tgcttcaatt ccgaatgata aaaaaatgat tgttacaagt gaaggatgct ttaaatattt
     1501 ctcgaaagcg tataatgtgc cttctgctta catttgggaa atcaacactg aagaagaagg
     1561 aacaccagat caaataaaac acttagttga aaaattacgc acaacaaaag ttccctcctt
     1621 attcgtagaa agcagtgtgg acgatagacc aatgaaaaca gtatcaaaag ataccaatat
     1681 tcctatctat tcaacgattt ttactgattc aattgcagaa aaaggacaag atggtgatag
     1741 ttactatgcg atgatgaaat ggaacctgga taaaattgct gaaggccttt cgaaataatt
     1801 tggagggtag gtatggtaca aattaaaacg actactatac taatttattt actagcaatt
     1861 caggaattat caaaaagaaa aaagaaatta aaaaataatg atttagcaaa agttctaagt
     1921 gttagtccag catctgtaag tgagatgctg gctaaactta gatatgagga ctatttagat
     1981 ataggatttc aattaactga taaagggaaa aaatttattg acgattacaa aaaaggtttt
     2041 aagtaaaaga ctttattttt agggaggata agaaaatggc tattggagtg attatgatag
     2101 gacttaccct aatagctgga tataattttt ttaaatcttt ctcggattat tttagaaaag
     2161 gtgaatgatt tagatgagtc tattaattct tgtaggattg ctttttgtat cagttatttt
     2221 gctctttctc attagtttag ctctaaggaa tgagttcagt aatagacgtt aacgattgaa
     2281 acttcaagtc ctgtgtaaga taatatagtt aattcatctt attaggagga agtacccata
     2341 gaaaccagag attatattac aactcaccaa aaattaatag aaagtggact aagccatttt
     2401 gaaaaagaag gatttcagca aactaagata aggcaactgt gcttgaccgc aggcgtaact
     2461 acgggtgctt tttataaaca ttttacttct aaggaagcgt tatttgaaga aattattcag
     2521 ccgcatatta atgcgcttat taatatgtat aatgaagagt ttaccaaata cctcttaaac
     2581 ttagaaaata tagatataga gaaaagttgg agagagaact tcaatgaatt agatccattt
     2641 attaattata tttttgagaa taaacgtata ttcgatttgg ttttattcca atcagctgga
     2701 accaaatatc aagatattgt tgaaaaaatt acatcatttg tgacaaaaaa gacagtctgt
     2761 tgtctagata agttaaagac tatcggaatg atcaatcaaa atttagtctt taatgaagaa
     2821 gaaatccact tctacattta ctcattctac gctacattct atgatatatt gaagcatagc
     2881 tattcaaaag aaaagactat gctattaacc agaagattgt atcaattttc tgttcctggg
     2941 tggattgaat tactgagtta attaaaaaaa agcttgcatt gagaattgtt ctcagttatg
     3001 ctttttgata agatcgcact gttatctgaa taacggtgca tttttgtcag cttaagataa
     3061 cagtgcgatc ttattttttg gaggtgaaag agtgtcaaaa atcaatccgt taaccttgat
     3121 tactttaaat attttttttc cggtcattat ttttttagga aagggaaata tatttcaagt
     3181 ggggtgtttc attattgcta cagttttagt attgatgttt cgtaaaataa agacatgtat
     3241 ttatttcatc ttgatttatt cagttctgtt tttagtaatg aaaattattg atgctatcaa
     3301 gatagagttt tttactatat tttttgggac atccttatat attgtatttc gaatgttgcc
     3361 ggtcgtaatg atctcatggt tgttggttag tgtctatact agcaatgaat tattatcctc
     3421 tttagaaaaa atacatttac cgaaaaaaat aatgttaagt attacagtag tgatcagatt
     3481 ctttccgact tatcgttctg aaataagaat gattaaagaa tctttaaaaa tgagaaacat
     3541 tcgcttgtct ttttttcagc ctctgcaata tttggaattt ctgttagttc ccgttcttat
     3601 acgtgcaact atgattgctg aagaaatgac cgctaatgca ataacaaagg ggatagaatc
     3661 tcctgtgaaa agaagttctt actataaaac aaacatgagc ttattagact atatcatcct
     3721 gttttccgtt actatttttt tcttaagtat tttgatttga ggtgaaagaa ttgtttgaat
     3781 ttaacaatgt ttctttttca tataaggatg ttaaggaaca agcgcttgtt gaccttaatt
     3841 tttcaataaa aaaaggcagt cttacattga ttactggttc ttctgggagt gggaaatcca
     3901 cactattaaa gttaatgaat ggacttattc caagacttta tgacggaaaa ttggttgggg
     3961 aaattttatt tgaaaataac tcgctacaat ccttaacaca agaaagtata tcaaaaaata
     4021 ttggctacgt aagtcaaaat cctagaggac aattctttac aacaaataca acgagtgagt
     4081 tagtattttc gatggaaaac tttggattta caaaagagca tatggataaa agaatcaatg
     4141 aattagctga attattagat ctttcaaata tattagataa aaacattttt tcgctatcaa
     4201 gtggtgaacg gcagaaaatt tctattggat gtagtttatc gctaaactct aaattaatta
     4261 tattggatga accgtcttca aatttggact tcaaagcgac taagaaactt gctgacttac
     4321 tttgtgattt aaaaaataaa ggatttacta ttgttatagc tgaacatcgt ttttactatt
     4381 tgaaagaatt aattgatcaa gttctatttg ttaaaaatag aactgtaaaa actacaacta
     4441 ttcagttgtt aaaaactgaa aacaataacg cattaaggaa atttgacatt tttaatagtt
     4501 cgattgaagt tccctatatc gaaaaggtaa gtgcagatgt attaaaagtt gaagaaatta
     4561 cttatgaaaa tattctaaaa gacataaatt ttgatgtcaa agttggagat gttattggtt
     4621 tggtagggaa aaacggtgtg gggaaaacaa ccttactaag aatactcatg aagattattc
     4681 gccctaccaa aggaaagata atcgaaaata aaaaaaattt gtcatcgccg tttttagtaa
     4741 tgcaagaaat ggattaccaa tttttcacag aatcagtgag gagcgagtta gccctgggga
     4801 atgaaatagt tagtaaagat cagcaagaaa aaattttaaa aaaaatggaa ctttataaaa
     4861 tgaaagatca attaccgttt gatttatccg gtggagaaaa acaaagatta ttggtttcta
     4921 tagcttcatt gtcaaaaact aatttatttc tatttgatga acctacaagt gggttggatt
     4981 atatcaatat ggaaaggatt gctgacctta ttaaagactt aaaagaaaag ggtgcagtag
     5041 ttatagccac tcatgatccg gaatttcttt ataaaacatg caatagaatt atttatttaa
     5101 aaaatggtgg aataaaaaaa gatataaaac ttagagtaga agattcaact ttgatagaac
     5161 agatttttaa tgacataacg aaatagaatt gaggagaata tatatgaaaa caaaagatta
     5221 tatttttatt ggtttagcta cagttatttc cctagtaatt tacttttttt caataatggt
     5281 tagtagtatt ggtggggcgt ttggtcacag catatcccca gggatttttg gattattatc
     5341 tggaatcata tttgtttata ttagctataa ctaccctaga aaaggaattt ttactattta
     5401 cacaattgtt ttattgttat tttttacgtt aatgggcggg gcgtacttac cttggtttat
     5461 cagttcgatt agtatggcga tcttagctga tattattcta tctgtatttg gatatgatcg
     5521 agcaattcct caagttgtgt cttgggttct aatgcaacta ggcagtgctg ccggacagtg
     5581 gattcccata tggttcttta cagatcgttt tcgtcaagac tggatagata gaggacaatc
     5641 tgctgcaaca atggatgcaa tgatccacta cgcggtgggt atatggggaa ttatttcagt
     5701 gttagttgta gcaagtttat ctatgattgg tgtattgatt ggaagaaaag tcttaaaaaa
     5761 atacaaaaaa tagaacgtag tttaccttct atctgtttgt acatctctaa tttacgctat
     5821 tacggaaagc attaacagtt gtaaacgaat aagagaagaa attgaatgcg ggggaggaaa
     5881 aaatgaaagt atacaaaaaa ttatttagtt atgtccctaa aatgaaatac tacggatggt
     5941 tagccacttt attttctgat ttttctgtcg tattaacagt ctatggatat tacagtattt
     6001 acaaatttct tcagtctcta attattgtag gagatgaata tttagctaag agttatgcgg
     6061 ttcaaactgt tgcttggtta actttaggag ctttatttta tattttttct ggcttatttt
     6121 ctcatatcct tgggtttcgc ttagagacta atttgagaaa aaagggaatt agcggtttaa
     6181 cagaagcaag ttttcgtttt tttgatttaa attcttcggg atatattcga aagacaattg
     6241 atgacaacgc ggctaaaaca catatggcaa ttgctcactt gattccagat agtgctcaag
     6301 ccatagtaac acctgtttta tcagttgcct taggcttttt cattaacttt aaaattggat
     6361 tggttctaat cgccatgtta gtaattggtg tattcttgtt aaagaaaatg acagagaata
     6421 caaattttat tcaaaaatat caagaatcgt tagatgtgtt aagctctgaa acggtagaat
     6481 atatccgcgg aattcaagtt ataaagatat ttggtgctaa agtaacatca tttaaagcct
     6541 tgaataaagc aattaacgat tatgctaagt ttgcatataa gtattctttg agtgggaaaa
     6601 caccttttgt tctttttcaa tggctatttt ttggcatagt ttgcattttg atagttccta
     6661 taaccttttt tataacttct cttaccactc ccaagatctt agctgttgac ttgttgatgt
     6721 tgtttttttt aagtggaatt atttttgttt ctttcatgag aataatgtat gtaagtatgc
     6781 acttgtttga agcaagatat gcagtcgaaa cactagactc cctttattta gaaatgaagc
     6841 aagaccaact aaaatatggg aacgagacac agattaaaaa ttgtgacatt gcatttgaaa
     6901 atgtcagctt ttcatacaaa gaagagtgtg ttttagaaaa tttcaactta gttttagaac
     6961 aaaaaagaat ttatgcatta gtaggaaaat caggaagcgg aaagagtacg atagctaaac
     7021 taatttcagg tttctacaat gtggataagg gacagattaa aattggtggt aagcctttaa
     7081 atcactattc aaaagacaca attattaaca caattgcttt tgtctttcaa gatacgaaac
     7141 tattcaatca gagtatttac gataacgttt ctatagcaaa tcctcaagcg ccagcaagta
     7201 aagtatttga cgcgctaaga ttagccggtt gcgaatcgat tttagaaaaa tttcccgaaa
     7261 gagagcatac cttaattggt agtaaaggag tttatctgtc aggaggagaa aaacaacgta
     7321 tagctattgc cagagcaatt ttaaaagatg caaagattgt tattatggat gaagccagtg
     7381 catctattga tccagataat gagtatgaac tacaaaaggc ttttcaatat ttgatgaaag
     7441 aaaaaacagt tattatgata gctcatcgat tatccagcat taaatctgta gatgaaattc
     7501 ttgtcttgtc agaaggaaaa atagtagaac gtggaaatca taatgaatta atggctaaac
     7561 atcgagtgta taaacaattg gtcactaatt atgagaatgc aaatgattgg aggcttgaca
     7621 atgaaaaatt tttataaaaa aaaatttgct ttaactgatc agggcgcaga agctttaact
     7681 aaagcttcta tttctagttt ttttgtatac tgcattaata tggtaccagc attcattata
     7741 atgatgctga tagacgaatt agttttagaa aatgcaaagc cccgttggct ttattttgca
     7801 gtatcgtttg ttactttgct tttcatgtat tggttgttag atagggagta tgaaaactta
     7861 tataatagta cttataaaga aagtgcgcat ttaagagtgc aaattgcaga cgatttgtca
     7921 aatttaccat tatcctattt ttcaaaacat aatttatcag atttatctca aactatcatg
     7981 tctgacgttg aaggtattga gcatgcgatg agtcatgcaa tacctaaagc cggcggtatg
     8041 gctctgtttt tcccttttat ttcagtgatg cttttggttg gtaatgtcaa aatgggatta
     8101 gctgttattt tgccaacgtt atttagtttt gtcttaatct tgttatcaaa gaaatcccaa
     8161 acgaaagcca atactaaata ttacgatact ttgagagaaa actcggaaga atttcaagaa
     8221 actattgaat tgcagcaaga gattaatagc tttaatctat ctaaaaaagt tcaagacaga
     8281 cttttcaaaa aaatggaaga gagtgaaagg attcatttaa aggtagaatt aagtactttt
     8341 tcagtcatgg ccttatcctc tattttctca tatgttagtt tagcagtagt cattctagta
     8401 ggtgttcact tactgttaac gggtgaagtg actatactct acgtcgttgg ttacttacta
     8461 gccgcaataa aaataaagga ttccttcgat tctatgaaag aagcagtgct tgaaatattt
     8521 tatttagctc ctaaaataca gcgaattaga gctatgaagg aaaccagtat ccaagaggga
     8581 tcagatagtc ctctgaaatc ttttgacatt gagttgcgag atgtctcttt ctcatatgat
     8641 aacaataccc caattttaga tcatatctct tttacggcta aacaaggaga agtgactgcg
     8701 ttagtaggtg ctagtggttc cgggaaaact agtatcttga aattagtgtc tagactgtat
     8761 gattatgatg aaggctgtat tttaattgat gggtatgata ttaaacgtgt atctccagct
     8821 tctttgttta gcaaaatcgc tattgttttt caagaggtaa ctttgtttaa tacaagtatt
     8881 ttggaaaata tacgtattgg aaatagtcaa gcgtcagatg aggaagtgaa aaaagcagct
     8941 cgtttagcta actgcgaaga ctttattgaa aaactacctg acggttatca tacattggtc
     9001 ggagaaaacg gaagttcact ttctggagga gagcgacaac gcctatccat agcacgggct
     9061 tttttgaaaa atgcgccaat tttaatttta gatgaaatta ctgccagttt agatgcggaa
     9121 aatgaaaaac gtattcagga aagtttaaat cgtttaatac aagataagac agtactaatt
     9181 atatcgcatc gcttgaagtc tattgaaaag gtaaataaaa ttgtagtgat ggatcaagga
     9241 aaactagttg atcaaggtac tcataatgag ttgtatcgaa ggtcagaaat atacaaaaat
     9301 ctcataaaga aaactaaact atctgagaaa ttcgtctatg aaaaagaagc tcaatcacga
     9361 tgatgaaata ggctttaaat taacctacaa tttttgggat atagttataa ttctttttca
     9421 aaaagaataa ttttattaaa cagcatctaa ttttgaatac acgaaagaat cagtatgatt
     9481 gaattagttc acttaatgtc tattcttata atataaggaa taatcaaagt tttaaaaaat
     9541 tggcgataaa ccagttaaac ataaaagatt gaataaaaag ttatagtttc tttcatattg
     9601 ccgttttaag tagaggaaaa gctacttaaa acggtatttg tgtttgattg gactaataaa
     9661 agtgaattct tatttttcag agcctaaact gcatattctt ttaaatgatt tgattatctc
     9721 tatgtaccga taaattcgtt ttcaaatagt tatctattca tatgagtttt atttcatgtt
     9781 atttattgtt gacaagagca actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt
     9841 acgatttgag gtgtctaaat caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg
     9901 aaaattgcta aagcaaagaa acaaatggcg atgattgaaa agtatgcaga taaacgtcag
     9961 gaattaaaag ccgcaggaga tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg
    10021 aatcgtctga ggcttcgtga tcagacggat ggtcgtccaa gagggtatat gcgaaagttc
    10081 ggtatgtccc gtattaagtt tagagaactt gcccatcaag ggttgattcc tggagtaaaa
    10141 aaagcaagtt ggtaaaagga ggaaatatca tggcagtacc agcaagaaaa acatctaagg
    10201 cgaagaaacg gttaagaaga actcatcaaa ctgtagtaaa accagaaatt tcatttgacg
    10261 aagcgaatgg tgattataga cgtagccatc atgtatcttt aaaagggtac tataatggaa
    10321 agaaagtaat taaaggagct tcaaaatagt ttaatatata acttttagga gggaatcaaa
    10381 atggaaatta tttacccacc tttagttgaa gaaggattaa agtattatct tgaaacgacc
    10441 cagcaatcgc tagataaatg cactttttat cgttcgatgg tagagagagg aattattact
    10501 gaaacgggtt taccgaccca acaggcaatt gaaaatggat tagtcaaaga ttactatgag
    10561 gaccaaggct tatcatttga tgatttttta agaatctatc caatttttga agagtatgac
    10621 gaggagttat ttcaatgtat cgatggttat tgggagatac ctgttgatat gaaagaaaat
    10681 ttagtttcac aattggaatc tggcgagtta acttttgaag atgcacaaca aattcaagca
    10741 tatcttgaag atcgataagt attgaaggag gaagatttat gcgtcaaaat attattttgg
    10801 aatgtgtcga aactggagaa cgtctgtatc tcaccagcaa aaataaacgg aataaccctg
    10861 aacggttaga attaaaaaag tattcgccaa aattgcgcag acgggcaatt tttaaagaag
    10921 tgaaatagct aagtggtaaa aaggtgacaa aatgggcata ccagttacaa tcgtttctgg
    10981 gtttttaggg gctggaaaaa caacgctaat caatcaagcc ttgcaagtct caccctaccc
    11041 cagggaagaa attattataa ttgaaaatga gtttggtgaa gttgggattg atcacaaatt
    11101 attagttcat agcgaggaac gggtgttaca actaaataat ggatgtatgt gttgcagtct
    11161 tcgcagtgat ttattagcaa tattgatttc tttactggaa acaatagaag aacatcaaca
    11221 accaatttca caaatcatca ttgaaactac aggtatagca gatccccaac caatcattca
    11281 aacactttta acagcgcctc aactaagagg aagagtatat atcgatagtt tattgactgt
    11341 tattgatagt gacagttttg aacttattga aagagaacca caagccctta agcaacttgt
    11401 catggcagat cgcatttttg tgtctagaaa acaaaacttg aatttatctg cactaaaaaa
    11461 attacagaga gaaatcaaaa gtattaaccc tttatccgat attcgaatgt tcacacatag
    11521 tgaaacgatt caaaaaaagg atttttataa tttgaaaaaa tttaatcatc ccttagtttt
    11581 tgaaagtgat tcatcagaaa atcactataa tggcaaatac tatcatcaac accatcaagt
    11641 acatgagttt aaatcaattc tcttaacttc agatcaagac ataaaaaaag attttttact
    11701 ccaatggatt gactggctga ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt
    11761 gattgcttta caagagcagg attttatagt tgccatgcaa gaggtaaacg aacaggttaa
    11821 ttttcaatac accactcgac aaaaagacca aaccactatt attctaatcg gaaaaatgtt
    11881 ggatgaagaa aggatcaaac agagttttga taaattactg gcggaaagca aataatgata
    11941 agtagtgatt gggggtaatt tctaatggag aaaacggata tatctagtgc atatcgacga
    12001 cttaaaagcc cgaatattaa aactagaaaa agggcattaa aaattatcaa agaacataaa
    12061 cgaaataaaa tgaagaaatt agcataatta aggaaggaaa aagaatgaag aaaatcagtg
    12121 taggacttat tgggatagca gctctaggat tattgggagc ttgctccagc actaatgatg
    12181 caaaagtatc gaatgataaa gatggaaaat tagaaattgt aacaacattc tatccaatgt
    12241 atgatttcac caaaaacatc gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg
    12301 gatcagaacc tcatgactat gagccctcag ctaaggatat ggcaaaagct catgatgctg
    12361 atgtttttgt atatcataac gaaaacatgg aatcatgggt accaaaagct aaagaaagtt
    12421 ggaagaaagc tgggcctaat gtagtagaag gaacaaaaga catgatttta ttgccaggta
    12481 gtgaagaaga agatcatgat cacggagaag aagatcacca tcatgaacta gatcctcata
    12541 cgtgggtttc acccaaaatg gcaattaagg aagtttcaaa tatcaaggat cagttagtta
    12601 aattgtatcc gaaaaaagca aaagtatttg agacaaatgc tgaaaagtat ttgactaaac
    12661 tgaaacggtt ggatgctgac tataccacta gtcttaaaga agcgaaacaa aaaagttttg
    12721 tgacgcaaca cgcagccttt ggatatttag ctttggatta tggattgaaa caagttccta
    12781 ttgctggact aagtcctgaa gaagagccat cttctggtcg attagctgaa ttaaaagagt
    12841 atgttaaaaa aaataagatc aattatattt actttgaaaa gaatgctaat gataaaattg
    12901 caaaaacttt ggccaacgaa gctgggataa aattggaagt actgaatcct cttgaaagtc
    12961 ttacaaaaga acaaatggat aatggtgaag attatgtttc agtaatggaa gacaatctaa
    13021 aagcgttaga aaaaacaact atggttgctg gtgaagaggt agttccagaa aaagaagcga
    13081 aggatgaaaa gacagttgct aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat
    13141 tgagtgatta tacaggcgaa tggcaatctg tttatccgtt gttaaaagat ggaattttgg
    13201 atgaagtttt cgattacaaa gcaaaaatca ataaagacat gacagctgcc gagtacaaag
    13261 attattatac tactggctat aagacagata ttgatactat caacattaaa gacaacacca
    13321 ttgattttgt agtcaacggt gaacatcatc aatacacata caaatatgtt ggatacaaaa
    13381 ttttgaatta tgaaaaagga aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag
    13441 gtgctggtcg ttttaaatat attcaattta gtgatcatgg aattgcacca agtaaggcag
    13501 agcatttcca tattttcttt ggtggagaaa gtcaagaaaa actctataat gaattgcata
    13561 attggcctac tttctatcca gcttcattgt ctaaacatga aattgctcaa gaaatgatgg
    13621 ctcattaaaa ttcaattttt ggaggaaata catggtaaga aaagtattat tgtctatgga
    13681 ttccaagaat attgatgctt atcggcgtta tcgatttgca aagcaaatga gattaaatgg
    13741 gagtacatta gctgatattg ctgcaacttt acatgttagt attgacacag ttcatagagt
    13801 attaaaacgg gatcctgatt gttgttttat caaggtcaat actggaagaa gtagacgttt
    13861 agcaaaacgg cataatagct tggccaatta attgataata ccagagtaga tcatttttcc
    13921 cttatttgga tttgatctgc tttttattat gtcttgaatt gtcaaatcaa gcgcaacact
    13981 ttatcaaaga agacttcata aggtgttctc caatctaaac actttctagg tctgttattc
    14041 agtttttcaa tcatctgatt tatcgttgag ttatctaccg tagctatctc tttcgatttt
    14101 ggcaagtatt ctcgaatgag tctattcgta ttttaatttg ttcattgttg ccaagggcat
    14161 gaggatctgg aaaataaaag ggcatattat tcaatgcttc tgtaactgcg aaatgattgg
    14221 agaattcttt tcctctatct ggagtgatcg ttttgaccgc ctcatttgga atactgctta
    14281 aaagttcaat cattttctcg ctcacataag cggctttctt tctggggacc ttctttcctg
    14341 ctaaaaggaa gcatgatttt ctgtctgtca aagtcactaa acaagtacca ccagttttgc
    14401 cagctacaat atcggctttc caatgaccaa aatcacttct atcatctgcg gattttggac
    14461 ggccatggat cgaatgagta aggcaaatct ttccacgaga ctcgacatga ttttttgtat
    14521 gtcttgtttt tcctcgatgg tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg
    14581 gttctgtatc gaaaagatca ttgcaaattg cacgttagat cgtgataaaa ctaattgaaa
    14641 agtccatatg ttcatgcttc aaacgattcg agatttcctc tggcgaccac tgaaaatcga
    14701 caaataaatg acgaacaatt gaccaaactc acggatcatt tagaatgtga taacgtccgc
    14761 atttttttct tcgataagca taattttttt ccgctttgga aggtgaatag tttttttctt
    14821 gtttcttctc agttcatgta aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat
    14881 actaatagaa aatccttctt cgtgaagcag aaagattctt tctcgttcga gtatagtaag
    14941 atgacgatac tggctcataa ttattttcct tctaggagtg ttctcgacaa acttatacta
    15001 gttggatatc gattatgact catttttatg tgttgcactt aaagtgtaaa ttcaaggtat
    15061 agaaagaggt tccctatgaa aaaaatacct attacaatct taactggcta tcttggttca
    15121 ggaaaaacga tacttctaaa tcatattctg tacggtgatc acggaaagga aattgctgtc
    15181 attgtaaatg acatagggga agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt
    15241 aaaagaactg gagaaaaact tgtttcgatg ccgaatggat gcatttgttg tacattacgc
    15301 gaagatttga ttgttgaact agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt
    15361 attgaagctt caggaatcag caaaccaatt ccaattattc aagcaattat attagctaaa
    15421 aaccctttag acattgattt gacccaacga ttagagattg gtgccattat tatagtagtg
    15481 gatgtaaaac gtttttggga taatttcgaa acgggtgaaa agattcagga aagaaaagct
    15541 gaaggtaatg tagttgaaga agaacaggat attcgtggct tgttaattga tcaattcgag
    15601 tgttgtaaca tattactttt aaataatagt gacttagtgt caaccaagac gatttacaaa
    15661 attaacggtc taattgctaa gttacagtcc aatgccaaaa taattaggac aattaaaggt
    15721 gaagtagact tatcgaaaat tctaaataca aatcttttta atatagatga gacttcctat
    15781 tcagtgggct ggttcaaaga gttagagctt ggctatcaaa atcatattcc tgaaacagaa
    15841 gaatatggta tctcaagttt tatctatcga aatcggttac tccttgacgc acaaaaattt
    15901 gcccatctta cggataaaaa ttttcctggc aatattacaa gagcaaaagg gtttctttgg
    15961 tttgcccaac atagtgattt ttcgacgatc tttgaaacat cgggtagcta taaaagtatt
    16021 gaatcattaa attattgggt agctagttta cctaaaattg agtgtcaaat gattatacaa
    16081 aaaaatcccg aattgaagga agcctggcat ccgttgtttg gtgaacgcat taataaacta
    16141 gtttttattg gtatgaagat ggatcaacaa tgtattaagg aacaacttga gggttgttta
    16201 actgattctc ttaatgcaga taatttccga aaaatttcac catttccatg gaaaaagtct
    16261 aatatgttta gccagatatc gttaatgaaa tttttaagaa aagctggtgt caagataggc
    16321 gatgtcataa ttattggcat tttgattatt acctctttta ttcccttatt tgtattctct
    16381 atagaaaagg agaatgaaac aactgaagat gctatcaaat atgccatcgt tcgaattgat
    16441 ggcaaggaaa tggatcgctt cgatttggat aagatcaatc ataaactcgt gaattatcat
    16501 cctgcgaagg gacaatataa tattgttgag attaaagatg gaagaattcg agttaaagaa
    16561 gataatagtc ctgatcaaat cgcggttaga actggatgga tctcaaaacc cggtcaaacg
    16621 agtatttgtt taccccataa gttagttatt agtattgaga aaaaagaaag cacagattac
    16681 tacatttact aatctggaaa tagatgagga ggtttttaat aagaaaaaga tattattatt
    16741 aataatagca ggggctttat taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt
    16801 gacaacaggc acaagtatct ttaacgggac ctgcaatggt ttagttaaca gtcggttaga
    16861 ttacttattt tggctcttcg tcaatgagtg ttggtgaaga aattcaatag aataatcgag
    16921 atactcacac ggctagacag ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag
    16981 ttgggtagta ttcttagttt tcttaaaata ttttcctcga tataaaaaga ttcgttctcg
    17041 gtagttttga aagtttctat agccataggc aattcgtttt agtactttga tatggttatt
    17101 caaacattct atagggccgt tggaataggg aacgcgcaac gctcgtttga tttctgtttg
    17161 gtatttcttg aaggtcgtga acactggttg gtagtgctct ggcaactcct tgtagtcttc
    17221 ttttaggagc gcgacgaatt caggaacatc ttgtcttctg ataacggaaa gaaaatcctg
    17281 gtagacttcg tagcctcgct ttaaggaatc atcataagat aatagccgat cgactatctc
    17341 tgcttctgtc agatgatctc gaaatgacgg acgccagtag cgatgctgac ctttcaattc
    17401 ccacgcattc ttttgaagta gtttccagta tttctttaag tgcttcgacc gacgatcccc
    17461 ttttttaaag gtcttcatta cttgaattcg gtggttggta aaagcccgac caatgtgttg
    17521 gctaatatga aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt
    17581 gcgatagggt gtatagaaat ctgaaaccac tagacgcact tgttcacgga cttttatagg
    17641 gtagcgcaag aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag
    17701 ttgatgggtc ttgccgtcca tcatgacaaa gctcatggct cccgagacat ttttcactga
    17761 tttgaattca tcgaaacaca ccacttctgg taacagctgg ctcaaagaaa tggttttagg
    17821 ccgaaggctg cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcgat
    17881 caaagacata gaaagaggtt cgcttaatag ctcaagaatg gcttgtttga ctctgcgagc
    17941 aatggagcag cgacgatcac taactgaatc ttctgctaaa aaagtctttc cacagtgttt
    18001 acatttgaag cgctgtttgt taatccgcag aatggtcttg tagttactga catcattcaa
    18061 taagatggat acttttttcc agccctattt aatgatcgtt tgttcatttt gatagtggcg
    18121 atgaaagcat tcctccggtt gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc
    18181 attgattcgc tctttgcgaa aggcatcttc atgaaagata agatttaggt ctagaatatc
    18241 tagtaactct ttggtaaact ctgaatagga catcttgaaa cactcctttg aatggttttc
    18301 gtcgacttta ttctaaagga ttttaaggtg tctgtttgcg tttattagaa aaaagaaaaa
    18361 gtattggcaa cagaaggcgt caccaacact agatattata gatcccttat tttcttcgct
    18421 tcaaattgtc aaggagtcat cataaatttt gtatggattc ttttgtttgg gtagtagtct
    18481 tctaaataat cttggattaa caccttcatc tctattttac ttttatattt gttggagtcg
    18541 attaattctc atttgattga tcgatgaaac gcttcaatta ctgcattgtc aacaggagtc
    18601 ccagtttggc tcactgaatg tgtgatgtgg tggtcgttca acagctcttg ataaataaaa
    18661 gaccgatgct ggctacctct atcggaatga attaccgtag gcaattctcc ttaataaatt
    18721 tgtaatggac ggacgacaat ctcttttttc atgtgtgaat ctaccatata gtaacgtaga
    18781 atacgagttg ctaagtcaat ataggtggac aaatagaggc gaccgtcatt acatggaatg
    18841 taagtaatat ccatcaccca aacagcatca atttggttct gttcaaatta tcgattcaac
    18901 atttttttac catttctttg attgtttttt ctctaccatg agaagatttg cgtcgatgaa
    18961 atcctcttgt gcataaattc atttcacgca taagaacagc tacaacacgt ttattgattt
    19021 cgattccttc atcgtaaagt ttttgagtga tccctggaga tccgtatcga tctttgtgtt
    19081 cgtagaacac tcgtcttcct cttttagtcg tttattttcc tgttcaagaa tgtagaaatc
    19141 tgctttatca ggtaatcttt ttccttgtcc agcaaaagca tttttcccgt atcgacgata
    19201 ttctgaaacc catcgggaaa ttgttggacg agagatattg tattcttcag caattttctt
    19261 ggtagttact tttttctcaa ggatatcctg tgaaatcttt agtttaaatt tacgatcata
    19321 tgtttttctc aatagagttc attccttccg gtaacgtaac tgatagtcaa gaaaagtgta
    19381 tcatagccca acttagtatc agggtttgtc aactaaactg tggaagttga tagataagag
    19441 tttttaacaa ccaaaattct cttgactatt tttgaaatca gacaaatttt gattggacac
    19501 atagttgaat aaatctaccg tcgttacgga aggtaaatcg catgcttttc aattctagag
    19561 gagaagtcct cgttgaagta ccatagccca gttctggatt cgacctcctg cccacttcat
    19621 acgtaaataa agtaatttta ggagtgagac tgtgacaaaa gttattttta agctaaaaac
    19681 gaattaaaaa acgttgataa ttaaaatcat tcgttttaga ttctatgaat cattatagaa
    19741 cgctgtaaaa tcgatacttt ataattataa agaagatagt ttcaaattta ttgttcgaaa
    19801 tttgaaacta tctttttact tatgtcacag actcccttag ttgagttatt ctttaatttc
    19861 gaatttcgag atagctagat atattgcaag catacattct tgataacaat cttgatcata
    19921 ttttccagtt ctccaggatt gcttatgaat caaagagcta aatcttttga gaattgcctc
    19981 aacagcttct tcatttccag cctttgctaa aatgaaatct tcttttaaag aattcataaa
    20041 tctaccttct tcccatggac ttataatcta tttctttggg aaggaggaga acgagcaaag
    20101 aagcatacaa aattagttat tatgcagata ttaaaccagc attttttgaa tttttttgta
    20161 aagtcgatgt tttaaatttg tcgcaccctg tcttgtaatc cccagaacct ttccaatttc
    20221 tttatcagtt ttatcaaaga taaatttttc gataagaaaa atttgttcag gttctttcaa
    20281 caaaggaagg atttttgcta attttatatc ttcaacaagg agagtcacat ttaaaacatt
    20341 aatcggttgt ttaaatttta tagtatcttt agtaaacaaa tatggtggga gttgatcaag
    20401 ttgttcatgt ttttgaattt cttcttgttt tttataataa gtattagcgg tgttcaaaat
    20461 gaccttttca aagaatcgaa tcaactgggc atttacgcgt tcgtcgttat ctgacatatt
    20521 gcacacctcc tccattaaga tttaaaggga aaagaggcaa aaaacacgaa aagaagctaa
    20581 tgcaattatg ctgcgctgcg aataacttct ataagctcac atgcattgat aggactgctg
    20641 atgtttattt tcaaaaatgt ttttttagtt gataatggat agttatcttt atacttagaa
    20701 aagaaaattc atatatcttc ttaaatatca aacatgtcct tatggctaag aaaatgtctt
    20761 gagcttttta cgtataataa atcttttcta aaacctcctt ttggataata ttcttattcg
    20821 tgtatagatt gtatttttta tttcttcccc cttcataatt aacaactaga aaagagttcg
    20881 aatagacacg tatttgaacg aaaattttta ttggattccc aaattggaat atttagaaac
    20941 gaaaaattcc tattatctgt atagtgctta gataataacg gatcataata attattgtct
    21001 ttgaactaga tatatgtaaa tagtattcat catgatgaaa aatgatgagt tctcggtgat
    21061 aaaatgtact ttttacggtg gaatatacat gaaagcgata agtggatatt ttataatgaa
    21121 gtaagttagc ttattttgac agaggaatag tattttagat aacgtataaa atactattta
    21181 atgatgtaga aaggaaagag agaaacaaaa gtgttttcag ataaagaagt cttattaaag
    21241 tctcttcatg tttgtttaaa gttacctatc gtggttttta attccgaatt cagtattata
    21301 aaagagtatc gctcagatag aactacttat tttttttatg attataaaaa gttactaact
    21361 gataatatta aaaaaactaa agatttccat ttctttactg gtgattttaa tgagatgttt
    21421 cttctataca tgtttaaaac cagatattac ttatttggtc catttagagc aaataatatt
    21481 gataaagttt tttttaaact aaaaatgaac aatttgaatg ttgcaatggc tgatagagaa
    21541 cgactctata attcattgca aaatttgact ttgtattctt taggagacat tcgagatatt
    21601 ctgattttag ttcattattt ttttactgga aaaatagaag atctattcca tgaaccgtta
    21661 attgaatata cagaaaatct aagtaaaact atcgagcaaa tcaaaataga taatctgtta
    21721 tcacaaaatt acgatccaga aatatatctg tttctttatg aaaataaaat actggaatat
    21781 gttaaaaatg gagatattcg aaatttagaa aacatggttt ttaatctgag taatggaata
    21841 attccaagtg ttagtggaga tacgattcgg tctgaaaaaa attattctat cattgttttt
    21901 gaaaagttag cacaaacgtc tataacatta ggaatggata taatagaagc gtatcaaagt
    21961 agagatgctt tgatacaaga aaacgaatta gctgtatcgt tacctgaggt cttaaaagtg
    22021 agggattctg ggattgttta ttatactaag gaaatcggaa aaactaagat agagcatctt
    22081 tctcccttaa tttcttctgt cgttcaattc atcggcttaa atatctataa aagaattact
    22141 gtaaaggaaa tagctaatta tttttctgta agtgaaacta aattaagaga aagctttaag
    22201 aatgaaatgc atattacgat ttataattat atctctaagc gaaaaatttc tacggctaaa
    22261 ataatgttga aatcaaacca tactatcagt gaagtttctt taggtttagg attttcagat
    22321 tcttcacact tttcgagagt attcaaaaag tacgcaggtg tatctcctaa gcaatatcaa
    22381 ttaggtttag tggacaattt caacaacatt agctgagagg ttttatttta ttatgagact
    22441 atatcattta cgtagtttgg aggagttgag gaaattggat tttaacaaaa aatttttttg
    22501 ggaaatttct gatttgagtt gtttcgtaga atctgagcgt ctaagtaatg gtaataatag
    22561 tgtgttaaat gaatatgaca aagtagtaat taataaagta aaaagtttat gtgaagaagc
    22621 tagagaatct atcagtcata ttaatggtag caattttaaa caaacattat ttaatctgat
    22681 taagctagat gctaaaatat caagttattt attctttttg atacatgatg aatttatgga
    22741 ttatcaaaaa ttggatcagt taattgaaag agaaagctgg gaagattatt acgtgggtct
    22801 aactttttca gaaaagttaa acaactacaa gttaatagat tataagtatt tgagtgaaag
    22861 caattaatta ttcatgtaga gtactagaca ctttaaatat ggacaattca attataaaga
    22921 aaaagaatca ataatttaat gaggctgtga caaatgtcat tttttgtttc gctaaaaacg
    22981 agtgaaaaaa tgttgataac tccaatcatt cgtttaagat tctaggaatc attcagaaat
    23041 gctgtgaaac cgatacttta taattataaa gaaggtgatt tcaaattccg aagaaggaat
    23101 ttgaagtcac ctttttactt atgtcacagt ctcattcagt aatgcgggat aaggctcctt
    23161 attccgttat ttgaatactc tgaagtttaa tctaagaaag ggtaatattt tgaaaacatg
    23221 cttcatcgca tagaaatgaa cttaatttca gaaagaatac atacatatct ttttatatgt
    23281 tttcagctgc tgaatggtta aaaagcacaa atatccgaaa aaaacatgaa gaaatgtact
    23341 ttttttgttg taatgttcat gaaattctct aagtaaatta gttaacataa ggttagtaag
    23401 attcctattc tgtgaataat acgtatcaga tttatctttt attcttacta ggaaagtaat
    23461 tattaaggga gttgatttga ataatggtta gcaagaataa taagagagta tttctagaaa
    23521 aaacaaagaa acgagtattg aagtacagta tcaaaaaatt aagtgttggg gttgcatctg
    23581 ttttgattgg tgttggtctt gtcttgggaa caactgaact agttaaagca caagatgaaa
    23641 tttctccaag cagcccactt gaaacagcga taagctccgt acaagtaggt gacaaagtag
    23701 cttcggggaa tacttttcag gagaatcctg gttatacgaa aaattataac ttttcggatt
    23761 tacaatttag tcctcaagaa ctaactggag acacactaaa aggcaataca attggatttg
    23821 aagtttacgg aaaacataat attgcagcat caactaaaaa ctgggaaatc cgtcttcaat
    23881 tagatgaacg attagctaaa tatgtagaaa aaattcaggt cgatccaaag aaaggaattg
    23941 gatcttctag acggaccttt gtaagaatta atgattcgct gggtagacct acaaatattt
    24001 ggaaggtcaa ttatattcgt gcaagtgatg gattatttgc tggggcagaa acaaccgata
    24061 cgcaaaccgc tcctaatggg gtaattacgt ttgaaaagag cctagatgaa atttttaaag
    24121 agataggcat agacaatctc aagaccgatc gtttaatgta tcgaatctac ttggtaagtc
    24181 atcaagacga tgataaaatt gtaccgggta ttgatagtac gggctacttt ttaacagatt
    24241 cagatgattt ctataatagc ttagatgtat ctgaaaataa tcctgaccag tttaaacatg
    24301 gatcagtaag tgcaaaatat gagaaaccta atactcaaac aaaagatggt tcaggatcca
    24361 caggtgctaa tggagctata atcttagatc ataagttaac gaaaaattat aatttctcat
    24421 attcatcttc cgcaaagggc acgccttggt atgctaatta caaaattgat gaacgattag
    24481 ttccatatgt agcgggtata cagatgcaca tggttcaggc tgacaaagta acatatgatg
    24541 tttcgtttga atccggaaca aaagtagctg atttagcaat cgagcgacgg gaaggtcatg
    24601 aaaattatgg cataggttca ataactaata atgatttaac taaacttatc gattttgcta
    24661 atgcaagtcc acgtccagtc gttattcgat atgttttaca attgactaag ccattagatg
    24721 aaatcttaga agatatgaaa gcaacagcac aagttgaaga aaataaacca tttggtgaag
    24781 atttcatctt tgattcttgg ttgtcggata cgaataaaaa attaatccag aacacttatg
    24841 gaacaggtta ttattatttg caagatattg atggtgatgg aaaccctgac gataaagaag
    24901 agagcggaga cacgaatcca tatatcggga aacctgaatt agaagaagta tatgatgttg
    24961 acacaacagt taaggggaaa gtattcatcc acgagttagc gggaacaggt cacaaagccc
    25021 aacttgttga taaagaaggt actgtattag cagaaaaaac tatcgctcca aatgaaaaag
    25081 atggggctcc aatttcagat actgtagaat ttgaatttac gggtgtagat tcaagtaaat
    25141 taatcgcgaa agatgaatta aaaatccaaa tcgtttctcc aggttttgat aaaccagaag
    25201 aaggttcaac cgttattaag gaatcaccaa aagcggttga taaacaaacc gtggtagttg
    25261 gatttaaacc agatgctaaa gaatcaattc ggaataataa aaacttacct gaagatgcag
    25321 agtattcatg gaaaacagag cctgatactt ctaacgttac tgatagtacg aaaggtattg
    25381 taactgttaa gatcggaaat cgaactttcg acgtggatgt agagtttgct gtaaaagctt
    25441 ctcaagctat ggaaaatgat gcaacatacg tacctataac aacaactcca gaaacgacag
    25501 ttcaaagtgg aaaacctaca tttgataaac cagatgttcc tttagctaaa gatgcctttt
    25561 caatttcaga tgtctataat aaggacttcg gtaatgcaag tgttgatgca aacactggta
    25621 ttgttacatt cactccagct aaaggtgtag gagaatcgga gccgattact ggaacaattc
    25681 ctattaaaat tgtttaccaa gatggttctg taggcacgac cgatttagca gtaactgtaa
    25741 gtaaagatat ttatgaaaat ccaggagaaa acattcctgc aggctaccac aaagtaacct
    25801 tcaccgcagg ggaaggaaca agtattgaaa gtggaacaac agtctttgca gtgaaagacg
    25861 gcgtaagctt gccagaagat aaacttccag tgttgaaagc aaaagatggt tatacagatg
    25921 cgaaatggcc agaagaagca actcaaccaa ttacagcaga tgatacagaa tttgtatcaa
    25981 gtgcaacaaa actggatgat atcattgaaa atccaggaga aaacattcct gcaggctacc
    26041 acaaagtaac cttcaccgca ggagaaggaa caagtattga aagtggaaca acagtctttg
    26101 cagtgaaaga cggcgtaagc ttaccagaag ataaacttcc agtgttgaaa gcaaaagatg
    26161 gttatacaga tgcgaaatgg ccagaggaag caactcaacc aattacagca gatgatacag
    26221 aatttgtatc aagtgcaaca aaactggatg ataaatctga cgctgacaaa tataatcctg
    26281 aaggtcaaaa agtgactaca gaattgaata aggaaccaga cgcatttgag ggaattaaga
    26341 acaaagaaga tttaccaaaa gatactaagt atacttggaa agaaaaagta gatgttagcg
    26401 cagctggaaa taaaaaaggg actgttgtag tgacatatcc agatggatca tctgatgaag
    26461 ttgaagtaga tgttacagtg acagacaatc gttctgacgc cgataaatat gagccaacag
    26521 tagaagg
//
LOCUS       DACEUF010000036        25674 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.037, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000036 DACEUF010000000
VERSION     DACEUF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.037"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            170..514
                     /locus_tag="IXG20_001439"
     CDS             170..514
                     /locus_tag="IXG20_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ1609643.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="IXG20_001440"
                     /pseudo
     CDS             515..934
                     /locus_tag="IXG20_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1026..1376)
                     /locus_tag="IXG20_001441"
     CDS             complement(1026..1376)
                     /locus_tag="IXG20_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609644.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="IXG20_001442"
     CDS             complement(1342..2673)
                     /locus_tag="IXG20_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ1609645.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVNN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="IXG20_001443"
     CDS             2929..3495
                     /locus_tag="IXG20_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1609646.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="IXG20_001444"
     tRNA            3640..3727
                     /locus_tag="IXG20_001444"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /locus_tag="IXG20_001445"
     CDS             4061..5035
                     /locus_tag="IXG20_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1609647.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="IXG20_001446"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="IXG20_001446"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ1609648.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="IXG20_001447"
     CDS             complement(7450..7896)
                     /locus_tag="IXG20_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1609649.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="IXG20_001448"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="IXG20_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1609650.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="IXG20_001449"
     CDS             8361..8879
                     /locus_tag="IXG20_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1609651.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="IXG20_001450"
     CDS             complement(8956..9912)
                     /locus_tag="IXG20_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1609652.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="IXG20_001451"
     CDS             10078..10884
                     /locus_tag="IXG20_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609653.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="IXG20_001452"
     CDS             10881..11870
                     /locus_tag="IXG20_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1609654.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="IXG20_001453"
     CDS             11867..12871
                     /locus_tag="IXG20_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1609655.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="IXG20_001454"
     CDS             13003..13545
                     /locus_tag="IXG20_001454"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ1609656.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="IXG20_001455"
     CDS             13565..14263
                     /locus_tag="IXG20_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1609657.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="IXG20_001456"
     CDS             14284..14496
                     /locus_tag="IXG20_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ1609658.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="IXG20_001457"
     CDS             14496..15458
                     /locus_tag="IXG20_001457"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ1609659.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="IXG20_001458"
     CDS             15476..15871
                     /locus_tag="IXG20_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1609660.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="IXG20_001459"
     CDS             16034..16399
                     /locus_tag="IXG20_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ1609661.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="IXG20_001460"
     CDS             16396..18207
                     /locus_tag="IXG20_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ1609662.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..18436)
                     /locus_tag="IXG20_001461"
     CDS             complement(18269..18436)
                     /locus_tag="IXG20_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ1609663.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="IXG20_001462"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="IXG20_001462"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ1609664.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="IXG20_001463"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="IXG20_001463"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ1609665.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="IXG20_001464"
     CDS             21193..22173
                     /locus_tag="IXG20_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1609666.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22262..23086
                     /gene="tsaB"
                     /locus_tag="IXG20_001465"
     CDS             22262..23086
                     /gene="tsaB"
                     /locus_tag="IXG20_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ1609667.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23070..23612
                     /gene="rimI"
                     /locus_tag="IXG20_001466"
     CDS             23070..23612
                     /gene="rimI"
                     /locus_tag="IXG20_001466"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1609668.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LGVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23619..24083
                     /gene="rimI"
                     /locus_tag="IXG20_001467"
     CDS             23619..24083
                     /gene="rimI"
                     /locus_tag="IXG20_001467"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1609669.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24080..25096
                     /gene="tsaD"
                     /locus_tag="IXG20_001468"
     CDS             24080..25096
                     /gene="tsaD"
                     /locus_tag="IXG20_001468"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ1609670.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8327 a         4397 c         5377 g         7573 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gtaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 attgacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaagaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttattcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaata aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 acacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcaacc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatctc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgctttt agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt tttgctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat cggaaatgat tttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaattgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggta aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaaagaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagagatt ctgggctgga aacctgaata cacagatatc aaagcgatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattcc ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 accaattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gaagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaactt
    20641 gcttattgaa gcagctaact cagtattgga aaaatggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgcg attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgcct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccggcag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacactt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcagt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggcttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct cgctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg ggaaaatgga
    22201 gtgataccgc agcttttttg tgtttcagga aggggcaaaa tttgcccgcc agcagataat
    22261 catgtattat tgtactagtt ttgcctttgg tattttcatc ctcagaataa aaaagtatgc
    22321 tacaatggaa ggcgagaatg tcatagcaga aaggatcaaa tatatgatta cattaggaat
    22381 agatactgca aaccaaactt tggcgatcgg tgtggtagaa gacgaacaaa ttcttggaca
    22441 aatacaaaca aatatcaaac gcaaccacag tgtaacactg atgcctgcaa tcgaccaatt
    22501 gtttgcagat cttcaaataa gtccaaaaga cattgaccga atcgcggttt ctgatggtcc
    22561 cggttcttat acagggcttc gtataggtgt aacgacagca aaaacgattg cttatacatt
    22621 agataaagag ctagtaggag tatctagtct gaaaaccatc gcggctaatt gcatcggtgt
    22681 ggataatatt attgtgccca tgtttgatgc tcgaagaaaa aatgtatacg ctggggcata
    22741 ccgctggaaa gaaaatagat tagaaactgt acttgaagat gtacatattt ctgcttctga
    22801 tttgttcaca caactacaaa agctgaatga agctatttat ttcgtcggga gtgattgtca
    22861 caaattcttc tctgaaataa aggaaatatt gcctagtgcg atgattaata cagttccagc
    22921 atgggatatt ccaagtggtg taactgtggc tcaattaggt gctttagttg aaccagttgc
    22981 agagaaacaa gcttttcttc ctcgctattt aaaacgagta gaagcagaag aaaaatggtt
    23041 ggagacgcac acacctggag acgaaagtta tgttgaaaaa atttagagcg tatgccagaa
    23101 gcttttttca agataaaatg atttatacag aaaaaacagt cgaacaaatc gatcgtcgtt
    23161 acgtactaag agaattgact acagaagata tcaaagagct gctgggtgtc gaacgagaag
    23221 tctataatgg cgaattgcca tggacaaaaa gtgcctttat atcagaattg aagtcaccgc
    23281 ttcctcatct atatctaggt atctttgata atggaagaat gattggtttt atcggttcaa
    23341 gagcgttcgg tttagattgc catatcacga atattgccgt tcgaccggca tatcagcgta
    23401 aacgaatcgg ttcgctgctt attgacgaaa tcgagaactt tgctataatg aatcgctgtg
    23461 agacgatgtc attagaagtg cgtatgagta atcaagatgc acaaagactt tatcggaaat
    23521 tggggtttca agcaacaacg atcaaaaaaa gatattacac tgaaacaaaa gaagatgcgt
    23581 tggatatgat aaaactttta gaaaatgagt gagattagtt gatttgcaga aaaaaagatt
    23641 ttgatgaagc gtacttggca gaatgcgtat ggatgattag cgaaaatgct tatccacatg
    23701 gttcaccttg gacgaaaatg cagtttttgg cagacatcca acagcctcac tctgattatc
    23761 tgcttttagc tgaagataac cagatctgtg gctttattgg atactcgaaa gtaatagatg
    23821 aagtcgagat cacaaatatt gctgttgcag tttcagaaca aggaaaaggg catgcacggc
    23881 atttgttaca attactgatt acggaacaaa taaaagattc gctgcatgtt tttttagaag
    23941 tacgtctatc taatgaagca gcaagaaaac tttatgaatc agaaaagttt cgtatattag
    24001 gtaaacggaa atcatattat cgcaatccga cggaagatgc gatcattatg agcagaaagt
    24061 taaaaacgga gacgatgaaa tgaatgaaga attgattctg gcgattgaat caagttgtga
    24121 tgaaacgagt gtagcggtcg ttagaaatgg aacagagatc ttatctaaca tcgttgcttc
    24181 tcaaataaac agtcataaac gttttggtgg cgtagtacct gaagtagcta gccgccatca
    24241 cgtagaacaa atcaccttat gcttggaaga tgcactggta gaagctggtg tgtcggcaga
    24301 agatttatcg gcagtcgctg tcacttatgg accaggactt gtcggctcgt tgctgattgg
    24361 tatttctgca gctaaggctt ttgcttgggc acaccaattg ccgcttattc ctgtaaacca
    24421 tatggcaggg catatctatg cagcccgtct ggttaaacca ttccagtttc cattgatggc
    24481 acttcttgtt agcggaggac atacagaatt agtctatatg caagaagatg gatcatatga
    24541 aatcattggg gagacaagag atgacgcagc aggagaagct tatgacaaag ttggaagagt
    24601 attaggtctt tcttacccaa gcggaaaaga aatcgatcag ctagctcatc agggaaaaga
    24661 taattaccat tttccgcgag cgatgatcca tgaagataat tatgatttca gttttagcgg
    24721 attaaaaagt gcatttatca atttggtgca taatgcacaa cagcgtggag aagatctaga
    24781 caaaaatgat ttagcagcta gctttcaagc aagtgtgatc gatgtattga ttactaaaac
    24841 acttcgagct tgtcaaaatt atccagtcaa gcaactgatt atagctggag gtgtagcggc
    24901 caatcaaggg ttgcgtgaag gattgcagga tgctttggca actaaactcc ctgaggtaga
    24961 actagtgatt ccccctcttc gactttgtgg agataatgct gcaatgattg gagcagcagc
    25021 acatgtggaa atgcaaaaaa gaaattttgc tagctatcag ttgaatgctg atccaagctt
    25081 ggttttatct gaataaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    25141 ccaggaaaaa cggcttctca tgtttttgac ctggctgttc ttattaccgc gtatgcaagc
    25201 ttttgaacgc cgtttaatca agagttcgta agcctgtcgt ttaactctga ttcacaagga
    25261 acaatttcaa aaaacagctc ctcatgttta tgaacattat gacttgtgac cgaagagttg
    25321 acaggtacac accgtttaat cgaaataggg ctgtgacacg tttttgtcaa agccctattt
    25381 tttacatact tttaatcatt attgttattt tgctattttt tacagcttat ttatcattat
    25441 ccgttcctct gattgccctc actgagtaat cgtttattgt cgaggaatat cgaataatct
    25501 agatcggatg aggacaagac gcttcatcta gttaatctaa cagtattttt tgcaaacagt
    25561 gaatattaca taactgaaac acacatagaa aatctttgag tcttgtccag tgatctaaaa
    25621 ctgccggatc tttcagaagt tttagggagg aaaataagca gtgaccctga tatt
//
LOCUS       DACEUF010000037        25496 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.038, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000037 DACEUF010000000
VERSION     DACEUF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.038"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            129..3656
                     /locus_tag="IXG20_001469"
     CDS             129..3656
                     /locus_tag="IXG20_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1609671.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3653..7375
                     /gene="addA"
                     /locus_tag="IXG20_001470"
     CDS             3653..7375
                     /gene="addA"
                     /locus_tag="IXG20_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ1609672.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7444..7785
                     /locus_tag="IXG20_001471"
     CDS             7444..7785
                     /locus_tag="IXG20_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1609673.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8094..9137
                     /gene="pheS"
                     /locus_tag="IXG20_001472"
     CDS             8094..9137
                     /gene="pheS"
                     /locus_tag="IXG20_001472"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1609674.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9142..11562
                     /locus_tag="IXG20_001473"
     CDS             9142..11562
                     /locus_tag="IXG20_001473"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ1609675.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11732..12742)
                     /gene="trpS"
                     /locus_tag="IXG20_001474"
     CDS             complement(11732..12742)
                     /gene="trpS"
                     /locus_tag="IXG20_001474"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ1609676.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12996..13733
                     /locus_tag="IXG20_001475"
     CDS             12996..13733
                     /locus_tag="IXG20_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609677.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13747..14562
                     /locus_tag="IXG20_001476"
     CDS             13747..14562
                     /locus_tag="IXG20_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1609678.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14575..15222
                     /locus_tag="IXG20_001477"
     CDS             14575..15222
                     /locus_tag="IXG20_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1609679.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15238..15894
                     /locus_tag="IXG20_001478"
     CDS             15238..15894
                     /locus_tag="IXG20_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1609680.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16056..16877
                     /gene="racE"
                     /locus_tag="IXG20_001479"
     CDS             16056..16877
                     /gene="racE"
                     /locus_tag="IXG20_001479"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ1609681.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16880..18235
                     /gene="rph"
                     /locus_tag="IXG20_001480"
     CDS             16880..18235
                     /gene="rph"
                     /locus_tag="IXG20_001480"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ1609682.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18236..18754
                     /locus_tag="IXG20_001481"
     CDS             18236..18754
                     /locus_tag="IXG20_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1609683.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18861..19361
                     /locus_tag="IXG20_001482"
     CDS             18861..19361
                     /locus_tag="IXG20_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1609684.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19641..20561)
                     /locus_tag="IXG20_001483"
     CDS             complement(19641..20561)
                     /locus_tag="IXG20_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ1609685.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20726..21739
                     /locus_tag="IXG20_001484"
     CDS             20726..21739
                     /locus_tag="IXG20_001484"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ1609686.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21849..22604)
                     /gene="fabI"
                     /locus_tag="IXG20_001485"
     CDS             complement(21849..22604)
                     /gene="fabI"
                     /locus_tag="IXG20_001485"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ1609687.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22824..23264
                     /gene="fabZ"
                     /locus_tag="IXG20_001486"
     CDS             22824..23264
                     /gene="fabZ"
                     /locus_tag="IXG20_001486"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1609688.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23379..24140)
                     /locus_tag="IXG20_001487"
     CDS             complement(23379..24140)
                     /locus_tag="IXG20_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1609689.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24329..25423
                     /locus_tag="IXG20_001488"
     CDS             24329..25423
                     /locus_tag="IXG20_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1609690.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8448 a         4424 c         5382 g         7242 t
ORIGIN      
        1 tgcgtatact taaaaaagta gaaatatcag aaccttaaaa ggatataatt tgaagaatat
       61 agaacaaatg ttcagtattt tttgatataa taaaggagca ccgtatcatg tgttgaagga
      121 ggaaaagcat gtctttacaa tttattcttg gaaacgggaa aaaagatcac cgtcaagtct
      181 tgatcgacga agcatccgct tggctcgacc aggatgcaag taaccaagta tttttccttg
      241 ttcccaacta cagcaaattc gaacaggaac aagaaatttt agcggagatg cgaagaagaa
      301 gcggaaaaaa aactttcagc acaacaaacg ttcaagtatt tagtttttat cgtctggctt
      361 ggtatttgct gcaacagaca actcttctta caggaaatga attatcagaa tcaggatctg
      421 caatgatctt gcgacagatt ttagaaaaaa atgaggaaga attaacaatc tttcgcggag
      481 aaatcaataa aagagggttt attcgtcaat tacaagaact ctacagtgaa ttgcgtacag
      541 gaaatattca accagaagac ctgatgcttt tatttggtga ttcacctaat gcagaagacc
      601 agcaacttaa aatgaaagac ttgaagctga ttttttcagc ttatgacagc gaattgactc
      661 aacgtgcact aacaaatgaa gatgcactat ctatactagc aaattatatg cagacacaag
      721 atttcaacaa cgtcaaattt attgtcagtg gtttttctcg aatcaatggt caagagtatc
      781 aattactgca ggcaatgatg gaaaaggggc aattggttgt cgatttattg ctggatcgtc
      841 cttatacagc ggatcttcca gaagtactta cgctctttca tgagactgga aagctttatt
      901 tccgtctttt tcaaagtgca agagaacgga aagtccctat attagtggac cgatttgcac
      961 cagagcagac tcgtccgaca gagcttcaac tgctgcaaac aatgtgggaa gagacatcta
     1021 gccaaaagaa agcggcagta cacaaaatag ggggacaaga gcatctccat ttgtgggaag
     1081 ctgaaaatcc aactgaagaa gtccgacaat tagctgttga aatcagacga ctagtcagtg
     1141 aagaaaacta ccgttatcgt gatatccaag tgctgacaaa aaatatgtct ttatatggaa
     1201 atatccttcg gccgatcttt caagaaatgg caattccttt ttatatcgat gaagaacaaa
     1261 tgatggaaaa tcatccgctg atcgaatgga tcaacagtct gttcgctctt gaccgctatt
     1321 cttatcggct aagtgatatt atgcgttttt tcaaaactga attatttctg tcgacggaag
     1381 cagaaaccga acttgatgaa tggaaacaag agcgagatga attcaggaaa aaactggaca
     1441 tcactgaaaa tgtagcactt gcttacaatt atcaaggaac ttattggact cgtgaaaaag
     1501 actggcagtt tgtggcgtat gattttgaag cagaacagat tgaagacgta cagtatttgg
     1561 aagaacagtc aaatacgatt agacgattat ttcagaaagc tgtaccagta tttttgaaaa
     1621 aaataaagaa aagcaaaaca ggacaagagg ccgcaaatgt tttttatcaa tttttgatcc
     1681 aaagtggcgt acagcaacaa ttattgtttt ggcgtaatca ggaagtagac agaggaaact
     1741 tagaagcggc aagaaaccat gagcagacat ggggagcgct gattgatctg ttggatgaat
     1801 atgtagtaat ttatggtgaa tcggcttttg actggtcact gttccaagaa atcattacaa
     1861 gtggtctaga aaatctaagt tatggtaaaa tccctaccgc aatcgaccaa gtgcgaatca
     1921 atcgtttgga attagttcga ccaaatcaag ctgccgtcac ttttgcatta gggttgaatg
     1981 atcaagtgtt tccagaccga aaagagacaa aaggattgtt gtccagtgaa gaacgagaag
     2041 tattaaatca ggccttgcca gatggacagt ttttattcga cccatcaaag gaaagtatct
     2101 cttttgagcc tttccaagcc tatcttgttt ttctttccgg aacagatcga ttatatctta
     2161 gttatgcaca aagctatgac acagatggtt cgctgaagat gtctccttat ttgcgtagaa
     2221 tcacagaata tttatctgtt cccgtcgaga gaaaagagca tttgacgatc gaaagcgacc
     2281 ctaattgtta tgtagggact tacagaagcg gaatcaatac agtcaatagg atttatcgat
     2341 tagctttaga tgaaaaaaaa cctgtaccaa agcactggca aaagctcaaa gaattagtac
     2401 tgaattcgtc atggcgaaat tttgctttac agatttttga aagtcaggaa catttaaatg
     2461 ttcctgtttc gatgccagaa aaaatggcgg aagagcttta tggaaaagag atttatactt
     2521 caatttcacg tatggagaat ttttataatt gtgaatacaa atacttcatg caatttggtt
     2581 tgaaattgaa agaacgaact gtttatggtc taactccagc agcaactggt gatttttatc
     2641 atgaatcatt ggatcgtttc ttcaagctac tgttttcaaa ccaattatcc ttagtgaata
     2701 tgtccgatgc gcagcgtaga gactttactg aaaaagtgct gcaggaagta ttcggagaac
     2761 ttcggtttga gatattggat agttctgcaa gaatgaacta tatccgctac cagttaggac
     2821 agacgattca aaaagtgaca tgggctttgc aaaaacagag caaaaataca ggcatgcatc
     2881 ctcttcagac agagattcta tttggtcaga ttgctggagc aaaaggtatc acaggtctgg
     2941 aattaccgtt gagtaatggt ggaaaactcc atgttcgagg gaaaatcgat cgaatcgatg
     3001 tagcttctga acaagaagat acttggctaa gcgtcgttga ttacaaatct agcggacgtt
     3061 cattcgatgt cacagaagct tattatggca tggctatgca attattgacg tatctggacg
     3121 tagcattgat ggatgcggta cgtttgactg gaaaggcggc tgttaaacca gcaggatcat
     3181 attatttaca tgttcataat cctgtattga cggagacaga tgatgtagag aagaatacat
     3241 tgaaaaaatt tagttatgat ggcgtttttg tcgatgatcc cgctctttta gaagtactag
     3301 atcattcact ggaagcaaaa gaacaatcac tgatctatcc agtgaaaaaa aatgcgaaag
     3361 atgaatacca aaaaggtagt ttcagtaaag acaaattttt cacggaagaa gaactgcaac
     3421 tattcatggc gcataatcgg gaaaatatgc ggttagctgg agatgcgatt acttccggag
     3481 aaatcaaact aaatccagct tataaagata aacagcggat tgcctgcgaa ttttgtccat
     3541 tcagaagcat ctgtacgttt gatgtgatgc tgaaagaaaa taattaccac cgtctggaga
     3601 aaatagataa ggaagaagca ctgcgtagaa tattgaaaag gagtgaagaa gagtgaatca
     3661 aattccttta aaaccgcaaa atgaacgatt cacagatgat cagtggcaag cgatttttga
     3721 tcaaggagat aatctattag tctctgcgtc tgccggatca ggtaaaacta cagtgcttgt
     3781 tcgaagagtg attgaaaaac taaaaatggg ttttgatatc gatgaattat tgattgtgac
     3841 atttactgaa gctgccgcaa gagaaatgaa agaaagaatt caagaggcat tacaagaatc
     3901 cgtcaatagc gaaagtgacc ctgtacgtag acagcatttt actaagcagt tagttttatt
     3961 gcccacagcg aatatctcaa ccttacatgc tttttgcctg acagttattc gacgttatta
     4021 ttacttaatt gatattgatc cagtctttcg catgttaaca gatgaaacag aaacgatctt
     4081 gatgaaggag gatgtgtggg acgagctgag agaagcactt tatgcagaaa atgatgaacg
     4141 atttttccaa ttaacaatga atttttctaa tgatcgttca gatgacggct tgacgaatct
     4201 ggtgttttct ctttatgagt ttgcccgtgc caaccctgac ccccaaaaat ggctagaaca
     4261 gttaagcgat aattatcgtt tgcctgaggg gctagcgaaa tctcgattat atcaaaaaca
     4321 aatccgtcct ttagttttgg cagatatata ccaatgtgta cagttatatg aacagatgac
     4381 tcagttagca caaggtgaag gattagaaaa aatgcatgag caagtagctg gagaacaaca
     4441 gcagatcaag aatatctatg aagcgttctc gcaagatcgg ttagaagaag cttatgctgg
     4501 tttggaacaa ctaacttttt caacttttaa gagcagtcgg aaagcagaat tgaaagaaat
     4561 ttccaatgaa gtcaagggca tgagggataa agcaaaaaaa ctgattcagc agataagtaa
     4621 aagttatttt cctgtttccc caagccaaat ggaagaatta acggacaaag cactaccgtt
     4681 agtcgaagaa atgacaaaag tcacgcaaag cttcatggac ggtttcagta tgcgtaaacg
     4741 ggaaaaaggt gtgctggatt tcaatgatct ggaacattta gcgttgcaga ttctcacaga
     4801 aaaaacgaaa gatgtatggc tgccaagtga agcttcaaaa cattatcgaa aaaaattcaa
     4861 agaagtaatg gtagatgaat atcaagacgt gaaccaatta caagaagcca ttttatactg
     4921 gctaagagaa cccgatgata caaaaggaaa tatgttcatg gttggggatg tcaaacaatc
     4981 catctattct ttccgtttag cagatcctag tttatttatc gggaaatacg agaatttctc
     5041 aaagaaagaa ggcggacgtc gaatcgtttt ggctgagaac ttccgctcta gaaaagaagt
     5101 cctttctttt acgaatctta tttttgaaca actaatggat ccagcagtgg gtcaaatcaa
     5161 ttatgacgaa gcagccaaac tgatccaagg attttcagac tttccagaga atgaacagtt
     5221 tgagccggaa atcatgatct atgaaaaaga acaggaagaa tcagagattg agattccgac
     5281 ggatgatata ctggaagaca aaacagaagg cgaacttttt atgacaggac taaaaattcg
     5341 tcaattaatt gattcctcct ttatgattta tgataaaaaa tcaaaaaaaa gtcgtccgat
     5401 agagtacaaa gacattgttc tcttgacacc tacaaagaag aacaacttaa cgattttaga
     5461 gattttcaaa acattggata ttccattaga gatgaatgat gctcaaaact attttcaagc
     5521 gacagaaatt cgcacgatga tctcgctgct acaattaatc gataatccct atcaagatat
     5581 tccgcttgcg gctgtattgc gctcaccgat cgtgggtctg attgaaccag aattagccag
     5641 cattcgatta gctaaccgtg cacatactta ctatgatgct gtgcttgctt atcaagcatc
     5701 aaacgaagat gaattagcag ctaagctgga acattttggc aagcaactag agcattggcg
     5761 agaacttgct cgccgttctt cgatcactga cttgttatgg gatatctatt atgagacggg
     5821 atatctagag tatgtggtcg gtctgccagc aggggcacag agacaagcca atctttatgc
     5881 attggttgat cgtgccaaag cttacgagca aagttctttc agaggattat accagttcgt
     5941 gcgtttcatt gaaaaaatgc aagaaaaaga taaagactta gcagaaccag tcatttcaat
     6001 agaagataat gctgttcgcg ttatgacgat ccatgcaagt aaaggcttgg aattcccagt
     6061 tgtatttttg ttagatatga caaaggagtt caacttacaa gacttgcgca atcggtatgc
     6121 ttttgaagaa aaacttggag caggaattcg ctatatggat cctgaaacac gagtgttata
     6181 tgatacgtta ccgtttcaag ccatcaaatt ggcaaagcaa aacaaattgt tgtcggaaga
     6241 aatgcgtaaa ttgtatgtag gattgactcg ggcagaacaa aagcttttca ttgtcggttc
     6301 ctataaaaat aaagagcaaa tgatccaaac gtggtctgaa gcagctgatc atgaggagtt
     6361 agtttttgat cctgcgctac gtttgaaggg cagaagcagc ttgatgaatt ggattggtta
     6421 cggattgatc cgtcatcctg aaatgcagaa atacttagag gaagaaattt cgacttctct
     6481 tcttcaacat tcaaatgcac agttttctat ttcatggatg aatcagcaat cgatcattga
     6541 acaaagacag ttgcttgctg aaaaagaact ggttaactta gatcagcaga tgaaagaaga
     6601 tgaaacttta ttagcagatt cgttacaaaa acgtctggca tatgaatatc cttatcaagc
     6661 ttcttcacaa accacaagtt atcaatctgt atctgaaatc aaacgattat tcgaagatcc
     6721 agatgataca caagaatcac gtttgactct ggaaagcagt cagaacaaag cagcatcccg
     6781 tcaatttcgt tatacgcaag aacaactagc agaaccaaaa tttttgcaaa aagatcggca
     6841 agtgtcagca gctgctgttg gaactgctac gcatgctttg ttacaattac tgccattaga
     6901 gatgccaact acagagagta tccatcaaaa attacaggaa ttggtaagga aacggttagt
     6961 agacgaaaaa gtcgcgaaaa aagtagatgt ttcttctatt atatggttct ttcaaacaga
     7021 acttggtcaa cagctgattg ccaacaaaga aaacgtaaaa cgagaacaac ctttttccat
     7081 gctgcttcct gctgacgaag tatttcaaga ctatccaaat caagaagatg aactattaat
     7141 ccacggaatc gtcgatggat acctagaaga aaaagatcat ctcaatatct atgattttaa
     7201 aacagatttt attttacatc cggatgatcc agcagaaata gatgcaatcg tccaaaaata
     7261 tcaaggacag ctaagacttt accaacaagc aatgtcagaa gctttgaata agccagtaga
     7321 aaatgttttc ctaattttgc ttcgagtgaa acaaattata aacattaaca aataattata
     7381 attgagcaaa cttgacttat gttttgtaag gattatgcta tactctggga agaggtgagt
     7441 atgatggagc taacacagac agaattcgtt ttatgcccta aatttgaaaa aacattctct
     7501 attttaggaa aaaaatggaa cggattgatt atcgatgtac tactcgaaaa cggtccgcaa
     7561 cgctttgttg agcttgcggc aaaaatcccg atggtcagtg atcgagtatt agtcgaacgc
     7621 ttgaaggaat tagaaaaaga aggaatagtc actcgaaact ttgcctgtag cgaaaagagc
     7681 cgttcagaat atctcttgac tgaaaaagga gcagacttgc aaaaagcgat gcgtcaaata
     7741 caagtttgga gcgaaaaatg gatcacagag gaagaatgcg gttgattttg taaaaaaaat
     7801 cttgtaaact atagtccata cgaataagta aagaacgatg acgaaaaaga gtagtttggc
     7861 taaaccagtt cagggagatc tgtcgcagac tgaaagcaga tcctgtgttt gaaaaatgaa
     7921 gttcactttc cgagttcgtc tgataaaatc agaccggcaa ttgtcgttaa cgaagtaaga
     7981 gtgcggtgta agtcatttat ccgcaaatta ggatggtaac gcgaagacct cgttccttaa
     8041 caggaatgag gtctttttgt tttgtatttg gaatttaaag gaggaacaag cacatgtctc
     8101 tacaagcaca attagagagc ttgaaagaag caacgctcgt caaaatcaca gcggcacaag
     8161 atttaaatga attaaatcaa atacgtgttg aaacgttagg aaaaaaagga ccgatcacag
     8221 aagtattacg tgggatgaaa gatctaagtc cagaagagcg accaaaagtc ggaagtttcg
     8281 caaatgagat ccgagatctt ttgacagaag agatcgagcg aaaaaaacaa gtattagaag
     8341 aagaagcgtt aaatgcagct ttggcaaaag aaacaataga cgtgacacta cctggtagaa
     8401 aagtaaaaca tggtacacgt catatcgtga cacaaatcat ggaagaaatt gaagatattt
     8461 ttgtgggtat gggctatcag atcgtagaag gatatgaagt agaatctgat cattacaatt
     8521 tcgaacggat gaatctgcca aaagatcatc cagcaaggga catgcaagat actttctata
     8581 tttcagacga gatattgatt cggacacata cttcaccagt ccaagcacgg acaatggaaa
     8641 aacatgattt ctctaagggt gctctacgaa tgatctcgcc agggaaagtc ttccgcagag
     8701 atacagatga tgcgacccac agtcatcagt tccatcaaat tgaagggcta gttgttgata
     8761 aaaacatcac aatgggcgac cttaaaggaa cattagaagt cgtaatgaaa aaaatgtttg
     8821 gggaagatcg tgaaattcgt ttgcgtccaa gttatttccc atttacggaa ccatcagtag
     8881 aagtagatgt cagctgtttc aaatgtggtg gtgccggttg taacgtatgt aaatacactg
     8941 gatggatcga aattttagga gctggcatgg tacacccgaa tgtgttgaag atgtcaggaa
     9001 tcgatccaga agaatattca ggctttgctt tcggccttgg accagaccgc gtagctatgt
     9061 tgcggtatgg agtgaatgat atccgtaatt tctatcaaaa tgacttacgt ttcttaagtc
     9121 agttcaaggg ggagtaagaa aatgttagtt tcatataaat ggctcaatcg ttatgtagat
     9181 ttatcgaatg taacaccaaa agaattagcg gataagatgt ctgtgacagg aatcgaagta
     9241 gagggagtaa cggttccaga agaaggcttg aaaaaaatcg ttgtcggtga agtcaaggaa
     9301 tgcgtaccac atccagattc tgatcacctt tctatttgtc aagtcgatat tggcgaagac
     9361 gaactgtctc aaatcgtttg tggagcgcct aatgtgaagg ttggaatcaa agtgattgtt
     9421 gcacttccta actcacgtat cgctggtaat gtaaaaatca aaaaaggaaa aatgcgtggc
     9481 caagtatcaa atggcatgat ttgctcatta caagaaattg gctattcaga tagtgttata
     9541 ccgaaagaat acgctgaagg tatctattat atgccacaag aagctgtaaa tggcgatcca
     9601 gtttttcctt atttggatat ggatgattcg attattgaat tatctatcac accgaatcgt
     9661 gcggatgcat tgtcaatgcg tggcgtagct tatgaagttg gggcaattta tcgtcagact
     9721 cctacattcg atgacgaaaa gctggtagaa tcctcacgtc ctgctagcga taaaattcaa
     9781 gtggaagttg aagataaaga agcagttcct gcttatcaaa tcagaatcat tgaaggagta
     9841 agaatccagc caagtcctca atggctgcaa aatcttttga tgaacgaagg cattcgtcca
     9901 atcaacaatg tagtagatgt gacgaactat attttgcttt tattcggaca accactacat
     9961 gcatttgact acgacaaatt gaaatcaaaa gagatctttg tccgccgtgc aaaagaaaat
    10021 gaaacattag taacactaga tggagaatct cgggaattga caactgacaa tctcgtcatt
    10081 actaacggca aagaaccagt tgctttagct ggtgtcatgg gagggttaga ctcagagatc
    10141 acgaatgaaa caacaacagt tgcactagaa tctgctttat ttgatccaat ttcgattcgt
    10201 cgtacagcaa aacaatttaa tttgcgtagt gaatcctctg cacgttttga aaaaggaatc
    10261 aaccatgcga ctgttggaga agccagtgag gttgctgcag caatgatcgt ttctcttgcg
    10321 ggtggggaag ttctacaagg cgcggtcaaa ggctcagaga tcaaagcaga agacgtatca
    10381 gtgaagattt cacttgaacg catcaatcaa tatttaggaa catctcttgt cgttaaagaa
    10441 gtaaatgaga tttttgaagc acttggattt agctatgaag aaaaggagta tgtttattcc
    10501 gtagtagtac caccaagacg ctgggatatc caaatcgaag cagatattat cgaagaagtt
    10561 gctcgtattt atggatacga ccagttgcca tctacacttc caagtggaga aacggttgct
    10621 ggaagcttgt caaaagaaca agaaactact cgtaaaattc gtacaatctt tgaaggaagc
    10681 ggattgagcg aagcaatcag ttatgcattg acaacagaag aaaaatcaag acagtttgtt
    10741 ataaatgaaa gtaatattac acgtttggac tggccaatga gcgaagaacg ttctgtatta
    10801 cgaatgaatt tgatcagcgg actattagat gatatcagtt ataacactgc ccgaaaaaat
    10861 acagagattg ctttatatga aattggtcgg gtgttcttcc aagaaaatga tccacgtaca
    10921 catctgccgc aagaagtaaa acatgcagca atcgctattt ctggtatttg ggaagaaaaa
    10981 gattggcaga cgaaagctca aaatgtagac tttttcacca tcaagggtct tttagaaaac
    11041 ttatttgaac aactaggcat cactgaagat gtccattatc aaacagtgac agaaatgaag
    11101 gaaatgcatc ctggaagaac tgctgcgatt taccttggcg aaaaatttat tggttttgtc
    11161 ggtcaagtgc atccaacgat tacaaaaagt tatgatattc cagaaacata tgttgctgag
    11221 tttgatttat ctgctatagt ggaagctgct caaaaaggga tcgttttcga agcagtgaca
    11281 aaattcccag cagtcagccg ggacatcgct ttgcttgtaa aagaaactgt gacaaatcag
    11341 gaattaacag aagttatccg ttctgctgcc ggaagatttt taacagatat ccaattattt
    11401 gatgtctatc aaggagaaaa cattgaaaaa ggacataaat ccatggcgta tagcctgaca
    11461 ttcgtcaacc cagaagcaac attgacagat gaagaaatca ataaagcaat ggaaaaagtc
    11521 gaaaaagcgt tggtagaaaa cctagaggca agtattcgct aagaaggaaa atatagtaag
    11581 taacgatcaa gtagcttttg aagaccatac atttgaaaaa aagatgtgac acaacttcgt
    11641 cacatctctt tttttgattt taaaaaagga atagcacaga gacgagataa aaaaacaggc
    11701 ctttacagcc tgctttttta gagtaaatga attaatggaa gtaattgatt cccattgctg
    11761 attttacttc agaaagtgtt tgtgcagcta ctttagcagc ttcttcacta cctttacgta
    11821 agatttccat aacagctgaa ggatcttttt ccaattcttc tcggcgcgca cggatcggtg
    11881 caaattcagc ttccaataca tcaatgaggt atcgtttgat ctttacatca ccaagtccgc
    11941 cgcggcggta atgagctttc atttcttcaa ttgcttcttt atctgttccg aatacatcca
    12001 agtaggtgaa gaccatattc ccttctactt gtccaggatc ttgtacatgg atgtggtttg
    12061 gatcagtata catactcatg acttttttct gtaagacatc ggcaggatcg gagatataaa
    12121 taccgtttcc taacgattta ctcattttgc catttccatc gattccaggt aatctaccca
    12181 ttccttttgg tgggaatacg ccttttggtt ccaccaagac ttcaccatat gtatgattga
    12241 agctttgtac gatttcctgt gtctgctcta gcattggttt ttgatcctca ccaacaggta
    12301 caagattcgc tttgaatgca gtgatatctg ctgcttgaga aacaggatag ataaagaatc
    12361 ctgtaggcac gctttcaccg aatttttttt gttcgatttc tgttttgaca gtcggattgc
    12421 gccgaacgcg tcctacgctg actaagttca aataatacat ggttaattca gctaattccg
    12481 gaatttgtga ttggataaat aaagtagatt ttgcaggatc taatcccacg gctaaatagt
    12541 ctaaagcaac ttgcaagaca tttgatgaaa ccttctcagg atttttagca ttatctgtca
    12601 gtgcttgcat gtcagcaatc atgacaaaca gttggttgtc ttcatctgcc tgcatagcca
    12661 ctcgggtttt tagagaaccg acgtaatgcc ctaaatgcag cttcccagtt ggtcgatctc
    12721 ccgttaaaat aatatttttc aaaaaagaca cctctttacg atttgtaagt ctgagcactt
    12781 aatatcttac cgtttttcct gaaaagaaac aacagttcct aaggaaaaaa caaggaaaga
    12841 ggataaaata actaatagat tagaaatgga agaaatattt atgggagaat aaacgttcta
    12901 agaaacttta caaattacta aactatcaga tataatgtat tttattatac atctttaaaa
    12961 gaaatcttac taaataataa tgggagtgta attctatggc aatgattaaa tttgatcacg
    13021 tggataaata ttatggcaag ttccacgcat taaaaaatat caatctcgaa ttcgagaaag
    13081 gagaagttgt cgttgtcatt ggtccttctg gttcaggaaa gagcacgatg cttcgctgta
    13141 tcaatggatt agagacgatc tcttcaggta agctgctgat taatgatact gacttgcatg
    13201 ataaaaagac aaaactgaca gaagtccgaa aaaacatcgg catggttttc caacatttca
    13261 atttgtaccc aaataaaact gttctggaaa acatcaaatt agcaccaatc aaagtgttga
    13321 agcaagacca ggcaactgca gtcaaaaacg cggagaaatt tttgaaaact gtcaatatgt
    13381 tagataagaa agattcttat ccttccatgt tatccggggg acaacagcaa cgggtggcaa
    13441 tcgccagagg tttagcgatg aatcctgaga tgctgctgtt tgatgaacct acttctgctt
    13501 tggatccgga aatgattggt gatgtattag acgttatgaa aaaattggct catgacggta
    13561 tgtcaatgat tgtcgtgaca catgaaatgg gctttgccaa agaagtggcg gatcgtgtta
    13621 tcttcatggc tgatggagaa gtattggaag acagcagaaa tgtgagagac tttttcgaaa
    13681 atccaaatga agtccgagca cagcaattta tcagtaaagt tatcaatcat tgataggagg
    13741 aaaaagatgc gcaggttaaa aaaaatacgc ctgattttcc ttttaggcat tgcttttctt
    13801 gcactagccg gatgtaaagg aaatgggttg gcaaatgaag atattttaga gcgaagcaaa
    13861 tctaccaatg aaatcatctg gggtgtcaag tatgatactc gattatttgg aatgatggat
    13921 attgagagca gaactgtaca aggatttgat gtagatattg caaaagcaat cactaaaaag
    13981 atccttggtg acaatggaaa aacagaattt gttgaagtca cttctaaaac tcggattcca
    14041 ttattgaaaa acggaaatat cgatgcaatt attgcaacga tgacgatcac agatgaacga
    14101 aaaaaacagg ttgacttttc agatgtctat ttcgatgctg gccaagcttt gttggtaaag
    14161 aaaggaagcc aaatcaaaag tgtagatgat ttgaatgctt ctacaacggt tctagcagtc
    14221 aaaggttcta catcggctgc gaatatccga cagcatgcac cagatgcaaa aattttagaa
    14281 ttagaaaact atgcagaagc atttactgct ttacaatctg gtcagggaga cgcgatgact
    14341 acggataatg cgattttgtt agggattgca gatgaaaatc cagagtatga gttagttggt
    14401 ggaacgttta ctaatgagcc ttacgggatt gcgatcaaca aagggcaaga aaacttcttg
    14461 aaagctgtta accaagcgct agaggaaatg catgcagatg gcacatacga taaaatctat
    14521 caaaaatggt ttccaaatga aactgaagga aaagtagagt aagggggaga ggcaatgagc
    14581 gaattgatac aaacttatgg accaatcttt gtcgacgggt tcaagatcac attgttttcg
    14641 agtgtcttag cacttgtgtt tagtttgatt atcggaacgt taatggcaat cttccaatta
    14701 tcaaagaata aagtagtgag cgccttagca aaggcatatg tagaattttt tagaaacatt
    14761 ccattattaa tcatcgtgat gtttttttat gttgtcgtac cgatttattg gtttaatcta
    14821 gatggattcc aagcaggtac gattggatta acgatctata catctgcttt tattgctgaa
    14881 acagtacgtt cagggattca aaccgtacca aaaggtcaaa cagaagcagg actatcttct
    14941 ggttttacct attcagaaat catgcgttat attgttttac cgcaagcatt caaaatcgtt
    15001 atcccgccat taggcaacca atttatcaac ctcgtaaaaa actcatctat tttagcaatc
    15061 gtagctggtt tagatttgat gtatcaagga gacttgatcg ctagtgaaac attcaatacc
    15121 ttcgatacgt atatcatcgt tgggttattt tacttagtta tcacgctacc gttatcttac
    15181 ttaatggttt atcttgagaa aaaatgggcg gttcgttcat agaaggaggg gaaatacatg
    15241 gattttagcg gcgctttttc atggatgaat atccgttttt tattggaagg attaaaggtt
    15301 actgttgaag tttcattgtt ttcgatcatt tttagtttct tgatcggtgg tctgacaggt
    15361 gtgattcgtt ttgcgaatat ccctttcttt tccaaaatcc ttggtgtagt gattgatatt
    15421 atacggaatc ttccattatt attgatcatt ttcttcactt actttgcctt acctcaaatc
    15481 ggcattcaga tgaacatctt ctggtccgct gtcgcagcat taacgatttt tgagtcagct
    15541 atgttgtcgg aaattttccg agctggactc aatgctgttc caaaaggaca gatggaagca
    15601 ggattatcaa cgggactgac ttatgtagaa tcgatgcgaa cgatcgtgtt accgcaagca
    15661 tttaaatcaa tgatcccggc aattgtgagc caattgattt cactgatcaa agatacttca
    15721 ttagcagtaa tcatttcttt acctgaactt tctcatcagg cacgtattat ttatggacag
    15781 aacaccaatt atgtgcttcc gatgttcatc atcatgacac ttatgtactt cgtggtatgt
    15841 tacgcgcttt ctctgctttc cggttacttg gaaaagagac ggtacagtta ttaatagatg
    15901 attcttttag cggttgtggc aaactaaagt cacaaccgct tttcctcttt ccattcgaaa
    15961 aaaagagatt tcgattgttt taagcaagta agagcacttg aaaattcacg ctaaaaagta
    16021 cacaataggg aggatagaat ttattgaaaa aaaggatgat acgattgaca gataatcgcc
    16081 ctatcggatt tattgattca ggtgtcggcg gcttgactgt agtaaaagaa gccctgaaac
    16141 aattaccgaa tgaaaatatt ttatttgtag gagacacagc acgctgccca tatggcccta
    16201 gacccgcgga acaggtaatc cagtatactt gggaaatgac ggattatctg gtggagcaag
    16261 gaatcaagat gctggtgatc gcctgcaata ccgcaactgc ggtggcttta gaagaaatca
    16321 aagctgctct ttctattcca gtcatcggtg tgatccttcc cggtactaga gcggcagtaa
    16381 aaaaaacaca aaataaacaa gttggcatta tcggtacgat tggtacggta aaaagtcaag
    16441 cttatgaaaa agcactgaaa gagaaagtac cagaattgac tgtgacaagt cttgcttgtc
    16501 ccaaatttgt ttcagttgtc gaaagtaatg aataccattc atcggtggcg aaaaaaattg
    16561 tggcagaaac attagctcct ttaaccacta aaaaaatcga tacattgatt ttgggatgca
    16621 cccattatcc attattacgc cccatcattc aaaatgtaat gggagaaaat gttcaactga
    16681 tcgattctgg agcagaaaca gtaggtgaag tatctatgct gttagattat ttcaatttga
    16741 gcaattcacc gcaaaatggt cggacattat gccagtttta tacaactggc tctgccaaac
    16801 ttttcgagga aatagctgaa gactggcttg gaatcggaca cttaaatgta gaacatatcg
    16861 aattgggagg aaaataagga tgcgtcatga cgggagaaaa gcccaagagc ttcgcaaact
    16921 ggagcttcaa acaaatgttt tcaaatatcc agaaggatcg gtagtgatcc gttttggtga
    16981 tacaacagtt atttgttctg caacaataga aaattctgtt cccccttttt tgagagaaac
    17041 aggaacagga tgggtgacag cagaatatag tatgctgcct cgagcaacga gtacgagaaa
    17101 tcgtagagaa agcagcaaag ggaaactttc tggtagaaca atggaaatcc agcgtcttat
    17161 tggtcgttcc ttgcgtgcag tagtagactt ggaaaaatta ggagaacgta gtatcatcgt
    17221 agactgcgat gtgatccaag cggatggcgg tactagaaca gcaagtatca ctggggcatt
    17281 tgtggcattg aaattagcca ttgaaaaact tttaagagaa aaagaattgt cagaagatcc
    17341 aataaaagaa catcttgctg cagttagcgt aggaatctta ccagatggga cttgcgtcac
    17401 agatttggat taccaagaag attccgcagc acttgtagac atgaatcttg taatgacaga
    17461 atctggaaaa ttcgtggaga ttcaaggaac tggagaagaa gcaacgtttg atggtgaaca
    17521 gttgaatgaa atgctctttt ttggaaaaaa cgcgattgaa gatttgatca aagagcaaaa
    17581 acatgctttg ctaacagagt ttgctcaaaa tgatgagcgg atagaagaaa caaaaacaat
    17641 catcattgcg acaagaaatc caggaaaagc agaagagttt cgaaatatgt tcaaagaagc
    17701 gggatatcat gtaaaaacat tacttgacta tcctgaattg ccagatgtgg aagaaactgg
    17761 cagcacattt gaagaaaatg cccgattaaa agcagaaacg attgcacaat tgcttgatca
    17821 gccagtctta gcagatgatt caggtttgaa agtggatgct ttaggcggta tgccaggaat
    17881 ctattctgca cgttttgcag gggaacaaaa aagtgatgcg gggaataacg ccaaattatt
    17941 atatgaattg acagatgtgc cggatgaaaa aagaacagca caattccatt gtacgttagt
    18001 attcgcggca cccaaaaaag acagccttgt agtagaagcg gaatggcccg gtcgagtagc
    18061 ccgtatacca tctggcgaaa atggctttgg gtatgatcct ttatttatcc cagaaggtaa
    18121 gaaacaaaca gcagcagaat tatcgagcga agaaaaaaac aagatcagtc atcgggctca
    18181 agcaatgaaa aaattgagtg ccgagtggaa acaatggctg gaaggagaga gatagatgcg
    18241 gtatttagta gtaagtgata atcatggaga cagagatatc ctcgttgact tagtagatcg
    18301 ttatcgttct gaagtggata tctttttcca ttgtggtgat tcggaattag aagcttctga
    18361 cccattatgg gatacattcc aagtggttca aggtaattgt gattatggac caggattcga
    18421 acagaaaaaa gtgatccaaa ctggtcagga tacagtattc atgactcacg gtcatttatc
    18481 gaatgtccga tttggtttga cacagttagc aatcgaagct cgagctgcaa ctgccgatat
    18541 ggtgtttttt ggtcataccc atcaaatagg ctgtgagatg gatcagaaaa tcctgttttt
    18601 gaatcctggt agcatttctc agcctagagg cccgatccaa atcccatcat atgctcttat
    18661 cgaaagttta gatgaccaaa tttctgttca atactataac cgtgcccatc aggcagttga
    18721 agagttggcc ttttcttttt cgagagagcg ctgaataaaa aaactctaat ttttaataaa
    18781 aataagcttt attttatgaa ttttgcatcc ggtttcggta aaatagtagt gttgaatgaa
    18841 atagaaatgg aggccatcag atgattggac ctatagtaag agaaatgctg ctggaaaatc
    18901 aggaaacgtt tttgattcct gcagaaaatg tggcaaatgt tatgtatcat catccattgt
    18961 cccatggttt acttgtgtta tcgaaggtcg gttattccaa aattcctgta ttaggaaaag
    19021 atgatcactt tgtaggacta gtaagtttat cagatgtggt caataaaatg attgacttgc
    19081 aaacaatcag catggacccg ttagaaggac taacggttgc tgatgtgaca gaaacagatg
    19141 ttccgacaat tgatgaaaat tgggaactag aagaagtact ccatttgtta gtcgattcgc
    19201 cttttttacc ggttgtcaca gaagatcgga ttttcaaagg aatcattaca agaaaagagc
    19261 tgcttaaagc cgtaaacttc atggtgcatg aattagaacg tcgtaatata ttgtctccga
    19321 aaatcgaaga attaaaagaa caaatcgata tcgtgagcta aagaacgaga aactaaaaga
    19381 taatcaaaaa ataagtagaa aaaacagcgt accagttttg actttatctt gaaaaagagg
    19441 ctgggacaaa agcgtttaac tccaagaaat aagaaggaat tcacgaaaat tgcttttcaa
    19501 atttttgtga atttcagctt atttccgaag gagttgcttc tgcttccgcc gtttatacgt
    19561 gtttagagcg tgggacaaaa gtgttcttta cttttgtccc acgctctttt tagtgagatt
    19621 cgttgcatcg tttgtccaaa ttattttccg acagcaattg gactgttcgg aatcgtgaaa
    19681 ccagaagctg tcaattcttt gacataggag ctcaagaact cttcttttaa ggcatattgt
    19741 tttccgttca atgcgtagca gaccgtacga atcgcaaaat tgctgtttcc aagatccact
    19801 aatccaaaga tagaaggttc agtttggagc tcttcttggt atttttcagc taatgtacga
    19861 tttactcgat caatgatctc atagattttg tcatatcctt cttcaggaac aatccgaata
    19921 tctaaaagta cttgcatatt tgcacgagag agattgctga tcgtggtgat gtttcgattc
    19981 gggatgaaat ggacagtacc atccgttgct ttcagctgaa gggtacggat accaacagat
    20041 acgacggttc cttcaatcgt taagtttgct agcttgatgt agtccccaac atccatctgt
    20101 tgttccatga taataaaaaa tccggtaata acatcactca tgaacccttg ggcaccaaga
    20161 ccgattgcca cacctgcaat tccggcacca gccaaaagtg atccgatcgg cacaccgata
    20221 gcagataaaa ttgcatagat aaaaaagaaa ccaagggtat aatgaaaaat agtatttaaa
    20281 agactatgta aagtctttaa gcggctctcg tttagaatgg cttttttaga atactgatta
    20341 aaagacttat caatcagata ttttcccaca cggtgaagaa tccaaaaaag gacgatcaaa
    20401 aaaatcagat ataaactttt ttggataaag gcagccaaaa tggcgtccca atcaatcccg
    20461 tcccaaaacc gctgaaacgc atttactttg ctgacagctt gatctgtcaa tgtctcgcca
    20521 gaagagtctg tcgtaccggc agttgctaat aatgaaaaca taaaatcctc ttttctaaaa
    20581 tttgattctt tctgtattgt accaagaagt tttcaaaaaa ggaaatagca aaaataacta
    20641 taatgaagga aaaaagctga aaataaaatg acaaataata tggataatga taaaatgtgg
    20701 agaaaataac gaaggtggta agtatatgca aatcgattgg aacaatctag gtttttctta
    20761 tatcaaaact ccttggcgtt ttattgccaa atggaaagac ggacaatggg aagaaggaga
    20821 attgacagaa gataattacc tgacgattca tgaaggctct ccagcgcttc attatggtca
    20881 gcaatgtttt gaaggtctaa aagcttatcg tcgaaaagac ggcaaggtca atctctttcg
    20941 acctgaacaa aatagtcgac ggatgaatca aagtgctaga cgattgctga tgccaatggt
    21001 ccctgaaaag atgttcgttg atgctgtcaa gcaagtggta aaagcaaatg aagattttgt
    21061 gccgccctat ggaacaggcg ctacattata cttgcgccct ttactgattg gggtcggtga
    21121 aaacattggt gtccatccag ctccagaata tttatttgtc gtcttctgta cgccagtagg
    21181 agcgtatttc aaaggtggac taaaaccaac gaattttatt gtatctgatt acgatcgtgc
    21241 tgctcctaag gggactggtg cagcaaaagt cggcggaaat tacgcagcga gtttacttcc
    21301 aggaacagaa gcaaaagaac tggattactc agattgtgtt tatcttgatc cagccacaca
    21361 tacgaaaatc gaagaagtag gtgcagcaaa tttctttgga attacaaaag acggaaaatt
    21421 catcactccg caatcgccct ctatcttacc aagtatcacg aaatattcct tgttgcagtt
    21481 agctgaagaa cgactaggac tgcaagcaat tgaaggagat attttcattg atgatctaga
    21541 tcaatttgca gaagctggtg catgtggtac agcggctgtt atttctccaa tcggtgggat
    21601 ttatcacaat aacaatcttc atgtatttta tagtgaaacg gaagtaggac cagtaacaaa
    21661 acggttgtat gatgaattga caggtattca atttggagac atagaagcac cagaaggctg
    21721 gattgaagtc gtggaataat ttcaaagtaa tgagttgaaa aaaacgtaaa aagaaaaaat
    21781 caggaagaga ctttaaaaag ttttttcctg atttttatat gttttacttt aaagaatgca
    21841 acaaaagttt atgaaagatg gacacctttg tcaacgtaga taatatcacc gatgattccg
    21901 ctggaaagtg ggctgattaa gaaagcacaa gcattaccga cttcttcgat cgtcacacct
    21961 tgctgatcag gcgtacgaga ttcagataac tggatcagtt tttgatagtc tttaacacct
    22021 gtgacagcta atgttttgat tgccccagct gagattccat tgacccgaat tcctttaggc
    22081 gagaactcag ctgccaaata acggatagca gcttcaagag aagctttcgc aatacccatc
    22141 atgttataat tcgggattgc gcgttctgaa cctagataag tcatgctaac gatcccgctg
    22201 tttggtgcaa gaaacggttg tgcgtatttt gatacagcaa ttaaagagta gctgctgata
    22261 tcttgagcta attgatagcc gtctctggaa atatcagaca catttccgga caattcttct
    22321 ttgttggcgt aagcaactgc atggacaagg ccgtggatct ccccagcata ttcaccgatg
    22381 gcattcattg ctttttcgat gctttcgtcg ctagaaacat cacattctac agtgaatgct
    22441 tcttcgccga ccaatttgac taatgatttt ttcattcgtt cattttggta agtatagata
    22501 atttctgccc cttgtttcaa cattgcttca gcacaacccc aagcaatgct ttttttgtta
    22561 gcgacgccca taatgacgat tttttttcct tctagaaatg acataatgac tctcctttgt
    22621 ttctgataaa aatttgatag tcgtaaaaaa atagagaata atagctaaaa taacttcttg
    22681 atttcttaat tattagtcaa aatgttttga ttgtcaaact attttttctt aaattaaaaa
    22741 aagattgcta aatatttagg aatatgatac tttgatagtc aaagtaatga aaatggcaat
    22801 tgcctgaaag aaggagttta attatgtctt acgtattatc tgctactgaa gtaatggaat
    22861 tgattcctaa tcgttatcct atttgttata ttgattatgt agatacttta gaaccaggaa
    22921 aaaagatcgt tgcgacaaaa aatgtaacaa tcaatgaaga ttttttccaa ggtcactttc
    22981 ctgacaatcc agtgatgccg ggtgcgttga ttctagaaac actggcacaa gcaggttcga
    23041 tacttatcct aaaatcagaa gagtttctag gaaaaagagc ttatatcggt gggatcaata
    23101 aggccaaatt ccgtcaaaaa gttgttccag gagatgtgct gaaatgtcat tttgaaatca
    23161 ccaaaatgaa gggatcagtc ggtacagcgt tatctgcagc atatgtagat gataaaaaag
    23221 tctgtgagtg cgagttcacg ttcatcgtca accaaaatgc ttgactataa aacctgatat
    23281 agcaaaacaa aaggaagctt acttgctttt tatgtaaaaa gaagacgcct gaatcctagc
    23341 atacgttgtc atctatgcag gattcagacg ttattttttt atttttgttt tgcttcttgc
    23401 agtatattga ttttatcgat ttttacttct tcttttggtt tgtctgaact atctgtttcg
    23461 atatttgcaa tagcatccac gacatccatt ccttcaatca cttggccgaa gacagtataa
    23521 tggccatcta gtgaaggata accgccgttt ttataagcgt cgatgattgc ttgaggatag
    23581 tcgtctttta aaagaccatc atgcatatca tctgtatttt gaacgataaa gaactggctg
    23641 ccgttactgt taggatcttg tgagcgagcc atagataacg cacctcttat attataaagg
    23701 tggttgttga tctctgtttc aaatccttct ccccaaatgc tttcccctcc agtgccatcg
    23761 cctttaggat cgccaccttg gatcatgaaa tctttgatga cgcgatggaa agtcagtccg
    23821 ttgtagtaac cgtcttttgc atgtgtcata aagttttcaa ccgcttttgg tgcgtgttcg
    23881 ggaaataatt tgattttgat tgtgccttta ttcgtcacca tttcgaccag atcttcattt
    23941 tcggcgacat ctttcgtcaa ttgtggtaat tccaatgcgt tcaaatcgac gctactggca
    24001 gtcgtgcttt ccgcagtaga tgtacttttt gaactttctg tagacgaact agaagaagtc
    24061 gtgttgtttg cagaacaacc agcagcaaac agcaatgctg ctgctaaact aatacttaaa
    24121 ataactttat ttttcttcat aactgttcct ttccaattta ctattttgtt atctttaatg
    24181 ataacaaaat agagggaaaa tacctactta aattattgga atttatcatt tattttaaaa
    24241 gaaaaagaaa aggtgtagac catttacttt gtgtttgcta taatgtaagt ggatacaaaa
    24301 acgaaaagat gcaggaggaa atggtaatat gggaaaatta ggaatctcga tttatccaga
    24361 acggtcaaca tttgaaaagg acaaagcgta cttggattta gctcataagt atgggtataa
    24421 acgggttttt accagtttgc tgcagatcaa tgatgataaa gaaaaagttc tttcagaatt
    24481 taaagaagta gttgattacg cgaatagcct aggcatggaa gtaatggttg atatcaatcc
    24541 agcgttattt gaacagttgg agatttctta cgatgactta tcttttttcc ataagatggg
    24601 tgcctacggg gtccgcttag atatcggatt cacaggagca gaagaagcaa aaatgacgcg
    24661 caatcctttt ggtatcaaaa tcgagatcaa tatgagttcg ggcacgaatt acgttgacaa
    24721 catcatgagt tattcgccaa acacggataa tttattaggt tctcataatt tttatcctca
    24781 tcgatactct ggtttaggat acgaacactt tgttttttgt tcagaaaaat tcagaaaata
    24841 taatttgaat acgatggcat tcgtcaattc gcaatctgct gagtttggcc cttggcctac
    24901 gcaagatggc ttgtgcacgc tggaagacca ccgagatctt gaaattgcga cacaagtgaa
    24961 gcatttaatc cttacaggat tgattgatga tatttctatt ggaaatgcgt acgcaagtga
    25021 agaagaattg aaagaaatgg cagaagcatt taacgcggat tatccaacat tgaaagtaga
    25081 tacggaagaa ggtattacag aaaacgaacg aatctgcttg tttgataacc ttcatagtta
    25141 tcgaggggat cgctcagaat atatccttcg ttcaacgatg actcgtgtat actacaaaga
    25201 taaagatttc ccgcctcata atacgcgaga tatgcatcat ggggatgtac tgattgacaa
    25261 tgaagggtac ggtcaataca aaggagaaac acaaatcgcc ttgaaggata tgaaaaacga
    25321 cggacgtgtc aatgttgtag gaagaatctc cgatgacgaa ttattcctcc ttgacttttt
    25381 gaaaccatgg tcaagcttta aattaattga aaataaaaaa tagataatta gttattgcag
    25441 aaataatccg ctagaaatag ccagttattt ctgctttttt attttgtttt tttata
//
LOCUS       DACEUF010000038        25358 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.039, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000038 DACEUF010000000
VERSION     DACEUF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.039"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(132..509)
                     /locus_tag="IXG20_001489"
     CDS             complement(132..509)
                     /locus_tag="IXG20_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609691.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /locus_tag="IXG20_001490"
     CDS             765..1493
                     /locus_tag="IXG20_001490"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ1609692.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="IXG20_001491"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="IXG20_001491"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ1609693.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="IXG20_001492"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="IXG20_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ1609694.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="IXG20_001493"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="IXG20_001493"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ1609695.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /locus_tag="IXG20_001494"
     CDS             4628..6067
                     /locus_tag="IXG20_001494"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ1609696.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6099..7142
                     /locus_tag="IXG20_001495"
     CDS             6099..7142
                     /locus_tag="IXG20_001495"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ1609697.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="IXG20_001496"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="IXG20_001496"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ1609698.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="IXG20_001497"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="IXG20_001497"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ1609699.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="IXG20_001498"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="IXG20_001498"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ1609700.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="IXG20_001499"
     CDS             10661..11575
                     /locus_tag="IXG20_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ1609701.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="IXG20_001500"
     CDS             complement(11708..13273)
                     /locus_tag="IXG20_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1609702.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="IXG20_001501"
     CDS             complement(13266..14717)
                     /locus_tag="IXG20_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1609703.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="IXG20_001502"
     CDS             complement(14731..15747)
                     /locus_tag="IXG20_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1609704.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /locus_tag="IXG20_001503"
     CDS             15967..17055
                     /locus_tag="IXG20_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1609705.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /locus_tag="IXG20_001504"
     CDS             17109..18635
                     /locus_tag="IXG20_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609706.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /locus_tag="IXG20_001505"
     CDS             18637..19842
                     /locus_tag="IXG20_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1609707.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="IXG20_001506"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="IXG20_001506"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ1609708.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="IXG20_001507"
     CDS             21340..22764
                     /locus_tag="IXG20_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1609709.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="IXG20_001508"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="IXG20_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ1609710.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="IXG20_001509"
     CDS             24337..25314
                     /locus_tag="IXG20_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ1609711.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8202 a         4317 c         5695 g         7144 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DACEUF010000039        25251 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.040, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000039 DACEUF010000000
VERSION     DACEUF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.040"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(3..1991)
                     /gene="treP"
                     /locus_tag="IXG20_001510"
     CDS             complement(3..1991)
                     /gene="treP"
                     /locus_tag="IXG20_001510"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ1609712.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            2214..2927
                     /gene="treR"
                     /locus_tag="IXG20_001511"
     CDS             2214..2927
                     /gene="treR"
                     /locus_tag="IXG20_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ1609713.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            3004..3450
                     /gene="msrB"
                     /locus_tag="IXG20_001512"
     CDS             3004..3450
                     /gene="msrB"
                     /locus_tag="IXG20_001512"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ1609714.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            3620..4222
                     /gene="ruvA"
                     /locus_tag="IXG20_001513"
     CDS             3620..4222
                     /gene="ruvA"
                     /locus_tag="IXG20_001513"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ1609715.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            4235..5236
                     /gene="ruvB"
                     /locus_tag="IXG20_001514"
     CDS             4235..5236
                     /gene="ruvB"
                     /locus_tag="IXG20_001514"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ1609716.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            5265..5843
                     /locus_tag="IXG20_001515"
     CDS             5265..5843
                     /locus_tag="IXG20_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1609717.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            5895..6377
                     /locus_tag="IXG20_001516"
     CDS             5895..6377
                     /locus_tag="IXG20_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ1609718.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            6494..6868
                     /locus_tag="IXG20_001517"
     CDS             6494..6868
                     /locus_tag="IXG20_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609719.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="IXG20_001518"
     CDS             complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="IXG20_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1609720.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            9167..9757
                     /locus_tag="IXG20_001519"
     CDS             9167..9757
                     /locus_tag="IXG20_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1609721.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(9885..11234)
                     /locus_tag="IXG20_001520"
     CDS             complement(9885..11234)
                     /locus_tag="IXG20_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1609722.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11402..12142)
                     /locus_tag="IXG20_001521"
     CDS             complement(11402..12142)
                     /locus_tag="IXG20_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609723.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(12155..13747)
                     /locus_tag="IXG20_001522"
     CDS             complement(12155..13747)
                     /locus_tag="IXG20_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1609724.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(14315..14611)
                     /locus_tag="IXG20_001523"
     CDS             complement(14315..14611)
                     /locus_tag="IXG20_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609725.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            14787..15512
                     /locus_tag="IXG20_001524"
     CDS             14787..15512
                     /locus_tag="IXG20_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ1609726.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15505..16347
                     /locus_tag="IXG20_001525"
     CDS             15505..16347
                     /locus_tag="IXG20_001525"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ1609727.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            16350..16871
                     /locus_tag="IXG20_001526"
     CDS             16350..16871
                     /locus_tag="IXG20_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ1609728.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(17124..17687)
                     /locus_tag="IXG20_001527"
     CDS             complement(17124..17687)
                     /locus_tag="IXG20_001527"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1609729.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            17938..18207
                     /gene="rpsO"
                     /locus_tag="IXG20_001528"
     CDS             17938..18207
                     /gene="rpsO"
                     /locus_tag="IXG20_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ1609730.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            18395..20521
                     /gene="pnp"
                     /locus_tag="IXG20_001529"
     CDS             18395..20521
                     /gene="pnp"
                     /locus_tag="IXG20_001529"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ1609731.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            20907..21173
                     /locus_tag="IXG20_001530"
     CDS             20907..21173
                     /locus_tag="IXG20_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1609732.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            21317..23308
                     /locus_tag="IXG20_001531"
     CDS             21317..23308
                     /locus_tag="IXG20_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ1609733.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            23662..24963
                     /locus_tag="IXG20_001532"
     CDS             23662..24963
                     /locus_tag="IXG20_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1609734.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
BASE COUNT         8136 a         4782 c         5182 g         7151 t
ORIGIN      
        1 gactaagatt ttacttcagc tttaccgact gcttcgcccg ggcgaacaag tcctgattgg
       61 tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag caacaataat gtcagttttc
      121 ttgccagctt gttctatttc tggcaaatcc atttcagcaa tcagggtctc tggtgtgaca
      181 aggtatcctt ctttcacgaa tacattgaaa ggaacaccct ttagttcaac agtatctaat
      241 cccatgtgaa caagtacttc cagcccctca ttagaaagaa taccaatggc atgttttgtt
      301 tcaaagacac tcgtcacttt accgtttact ggtgcataga ctttgccgtt tgacggttcg
      361 acagcataac cgtcacccat cattttttgt gagaaaactg ggtcatttac ttcagttatt
      421 tccttgatgg tccctgcagc gactgcgaat aatgtttctt ttgttgcaac tgccgtacca
      481 gttgctgaaa caggttcgaa agactgtggc gttgctgttg cgccatttgc tgtttgaagt
      541 tgtacatcag cagcattttc aggaacagca ggatcaatct tattcaaaat tccctgacgt
      601 ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa tgatcattgc gatgatgaaa
      661 ggtacccatg tttctgcttg gatagctagt attcctggca aaccgcctac tccgatcgcg
      721 ttagctcgaa cacccatcaa gttagcaaac atgccagcta atccagagcc gaccattgca
      781 gcgacgaaag gatagacgta tttcaaattg attccgaaca tagcaggttc agttacgcct
      841 aagtaacaag aaatcattgc tggaatcgag atttgttctt cttttttatt cccacgatgt
      901 aagaagatga tggctaagac tgcagagcct tgggcgatat ttgataaagc aatcatcggc
      961 cataaattcg ttgatccgaa atctgcaatc agctgcatat cgatggcatt ggtcatatgg
     1021 tggagaccag taatcactaa aggagcatac aggaatccga atacggcact aaacaaccag
     1081 ctgatagaag acgtcaatcc agtatttacg atatttgaga tccagcttcc gactgtccag
     1141 ccgataggac ctaaaataac gtgtgcagct aaaacagttg gtaacaatga aaacaatgga
     1201 acaaaaatca tcgagatcga ctgagggata tatttgcgga agaagatttc tagatacact
     1261 aacataaacc ctgccagcat ggctggaata acttgcgctt gataaccaat catctggact
     1321 tgagcaaaac caaaatccca aaatggaata tctgctgcgg ctgtggaagc tacactataa
     1381 gcatttaata attgcggaga aactaacgta attcctaaaa cgattcctaa tatttgtgta
     1441 gtacccatct tttttgcaat actccaagtg atacctactg gaagaaaatg gaaaattgct
     1501 tcacccggta accacaaaaa ggcattgaca ccattccaaa acttcgagtg ttcaacgatt
     1561 gttccaccaa gactttcaaa ctggatacct tcaagtacat ttctgaatcc aaggatcaat
     1621 ccaccgacaa tgattgcagg aatcagtggt gtaaagattt ctgctaaaac ggcaatagca
     1681 cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc cttgttcttt ggaaacccct
     1741 tcaactccag atactgcaac gaagtcgtta taaaaagtag aaacatcgtt tcctatgatg
     1801 acttggaact gtcctgcatt cgtgaacatc ccttttacgc taggtatatc ttcgatcgct
     1861 ttttcgtccg cttttcctgg atcattcagg acaaaacgca tacgagtcgc acaatgacta
     1921 actgcagcaa tattttcttt tccgccgata tcatgtaaca gcttctcggc atctgcttga
     1981 tattttgcca tatcaataaa cccctttctc ttaacttgtc tatgcaagtt gtttcttcat
     2041 ttactatact ttttttcgga gagagttgca agcgttttat taaaaagtac gagagaaaat
     2101 gatatcgttt taaaaagctt aaaataagct tttcttaaaa ataggaacat tgaaatacaa
     2161 aaagagctgt agtatagtga agaaatgatg gaagaacgag tgagggaaaa aacatgaata
     2221 agttcaatga aattttttta gatttggaac agaaaatttt agcgaaagaa tatcctccgc
     2281 attcgctttt acccagtgag aaccaattga tcaaaattta tggtgtttca cgagaaacaa
     2341 ttagaaaagc gctgaatctt ttgactacgg aaggttatat tcaaaaaaaa caaggaaaag
     2401 gttcgatcgt actggatcgg aatcgcttcg actttcctat ttctggattg actagctata
     2461 aagagctaca ggagacccaa cgtattccaa gtgaaacgat catccatacg ttagaagaaa
     2521 cagaagtgac aaaagcattg aacgagatca ctggctggga gatcggtgcg cctgtttggc
     2581 atttgatacg cgaaagaaaa atcaatggcg aagtagttat attagacacc gattatctat
     2641 taaaggaaat cgttcctatt ttaactccag cacaagcaca aggatcgatc tatgaatatt
     2701 ttgaaaatga gctttctttg acgattgctt acgcgcaaaa ggagatcaca gtagaagaag
     2761 taacggaaat catgaaaaca gcgatggatc ttcacgatga cacttatgca gtagtagtca
     2821 gaagcctcgt tcatctggaa gatacccgct gtttcgagta ttctgagtcg gttcaccgat
     2881 tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa agtgtaaata gttcaaaaga
     2941 aagcaaattg ttggcatctg tccactttgg cgctatgata ggatcacaaa gggggcattt
     3001 ttaatggata aaccaataag accagaaaaa gaagaactga aagaaaagtt atcacccatg
     3061 gcttatgcag tcacacaaga aaatgcaaca gaacgaccat ttagcgggaa atatgatgat
     3121 ttttacgaaa aggggatcta tgtagatatc gtcagcggtg aaccgttatt cagctctgca
     3181 gaaaaatatg atgcaggttg tggctggcca tcattcagca aaccaataac gaaaagacaa
     3241 gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa tagaagttcg aagcaaagaa
     3301 gcagattctc atctcggaca tgtgtttacc gatggaccag tagatcaagg tggtctacgt
     3361 tactgcatca attctgcctc actgaaattc attccatatg aaaagatgga tgaattaggt
     3421 tatggggaat tcaaatcact tgtagaataa tggaaataca caaagagcgg tacaatacgg
     3481 ggatttactc gatcgtccgc tcttttttta ttcaagcgat tgctatttga aagaaacgct
     3541 ccccagactc cttttttcac aaggtgaaat gtgctacaat aaagggaacg tatgtgtgca
     3601 aaagaaagag gactagaata tgtatgaata tctgacaggt gtggttacct ttatcaaccc
     3661 ctattacata gtagtagaaa ccaatggtat cggctaccag atcgcactag gaaatcctta
     3721 ccgctacagt agcaaactaa atcaaaaaat aaaaatttat gtccatcagg taatacgtga
     3781 agatgcgcat cttctgtatg gatttgattc gctagaagaa aagcaactgt ttttgcgatt
     3841 agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca atcatggcaa gcgaagacca
     3901 ctccggtttg attcaagcag tagaatccag tgatgtcact tatttgacga aatttccagg
     3961 agtagggaaa aagacagcac aacaaatgat cttggatcta aaaggaaaat tcggtgagct
     4021 gagtatcgat acacctttta gtctatttga tgaagcaaac acaaaagacg ctacagctct
     4081 atctgaagca atggaagctc tctcggctct tggttacagt gatcgagaaa tcaaacgtgt
     4141 agaaaaacaa ttaaaagaag tagataatca gacaacggat gagtatctac gccaagcact
     4201 caagctaatg atgaaaaaat aaggaggaga ccaaatgaca gatgaatact tgttatcacc
     4261 agaatctggc gaaaatgaac tgagtttgga aaaatctctt cgaccacagc tgctttctca
     4321 atatatcgga cagagaaaag tcaaacaaga acttcagatt tacatcgaag ctgccagaaa
     4381 tcgggaagaa cctttagatc atgttctgtt gtacgggcct cctggattag gaaaaacgac
     4441 gatggccatg gtcattgcta atgagatgac cgtaaatatt cgtacgacga gtggccctgc
     4501 tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa ctggaagcag gggatgtcct
     4561 gtttatcgat gagatccatc gattacctag agtggtagaa gagatgcttt attcagctat
     4621 ggaagatttc tatgtagata tcatggtagg acaagggcca accgcacatc cggttcattt
     4681 ccctcttcct ccctttactt tgattggcgc gacgacacgg gcaggtatgc tttctgcacc
     4741 tttacgagat cgatttggca tcatttctca tatggaatat tatgacgaac aggatctgca
     4801 ggagatcgtt ttacgttctt cggatatttt tcaaacagag atcattgaag aaggggcatt
     4861 ggaaattgct cgccgatcac gaggaacgcc tagaatcgct aatcgcttgc tcaaacgggt
     4921 tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat cgtcctatag cggatcaggc
     4981 acttactttg ctacaggtcg atcaggctgg attggattat gttgaccaga aattgttgcg
     5041 aacaatgatc gagttatatg gcggcggacc agtagggtta agtacgcttt cagtaaacat
     5101 aggagaagag cgtgagacgg tagaagatat gtatgaaccg tatctgatcc aaaaaggatt
     5161 tttaaagcgt acacctagag ggcgaattgc tactgcttat gcctatgaac attttggtta
     5221 cgactatcat atatgatttt tgcttttaga ttggaggaaa atgaatgagt gatacaacac
     5281 gtacgaaaca attgatcaaa aacgtattga ttgatttgtg ccaaacgcaa tcttttcgaa
     5341 aaatcagcgt ccaaaaaatc gcaaatgaag caaaaatcaa tcgacaaaca ttttattatc
     5401 attttacaga taaatacgat ttgcttcgtt gggtctattg tgaggatgca ttgcattatt
     5461 tagatccaca atgtctttca cttgataatt gggaagagca agcattgaaa gtgctgaaag
     5521 caattgctga gaagaaagaa ttttatgcaa atactgtgct ttctgaccca gaaattttac
     5581 aaaaagagtt ttctcaactg atccaaaaat catttatcaa gatttttgaa caaatggatg
     5641 aagaaagaca actttctgca agagacaaac tattttatgc tcgttttttt tcttacggtt
     5701 gcagtggtgt attagtcaat tggatcacga atgaatatga agaaacaccg atggaaatcg
     5761 ctatgcagct tttccgttta gccaaggata cggaattata ttcttatcgt ctttatgcaa
     5821 aagaagagtt ggaagaacag taatagaaaa attttttgtt ggaaaagcaa taatcgctaa
     5881 aaggagatgg ggaaatgaca aaaattttat ttgtttgttt gggaaatatt tgccgttcac
     5941 cgatggcaga aggcttgctg agaaaacgaa tagcaataga aggaagggaa gcagaatttt
     6001 ttgtggattc tgctgctaca agcacgtatg aagttggaaa gacaccgcac cctggaacga
     6061 aaaaaatatt aaaccaagaa aaagtagata tgacgaatat ggttgctcgg caaatcacac
     6121 cccacgattt tgaaactttt gattggatca ttggcatgga tcaagaaaat gtagaagaac
     6181 tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca tttgttttta agtagcattc
     6241 ctggaaaaga aaaagaaaat gttcccgacc catactatac caataacttt gatgagacat
     6301 atcacttgat cacagaaggc ctgaatcatt ggtggaatat ttggattgac gaaaataaaa
     6361 taacaaacaa tttgtaaagt tgacgctttc tttttaaggc gatgttagag catttccttg
     6421 tttttttgta aaatgttagg taaaataaaa ttaattaagt aacactttct tagctaaagg
     6481 aggagtgcat tcgatgaacg aagagacagt atcgccaatg aagaagctta cgaagttagt
     6541 tactcatact tcgtatcaac tttttgattc ttttgtaaat gacactgaag aacatgctaa
     6601 gaatgtacga ttgcaaggaa agtttattct tgagctgcaa aaagctgcta ctgaacatag
     6661 tctagttatc ttacaagtaa gagcgaatcg tttgagtagc aagtatgaaa cgatctcagg
     6721 ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg atccgcctac aaaacgaaga
     6781 gcagcagatc cggatggttc ctgtagaaca aattgaaaaa ataacaatcc tttcaccagc
     6841 aggggaacag gaacgattat caaaataatt tataccagat agaaaagcac gtatgagctt
     6901 gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt taagctgata tcagtaaaac
     6961 ggaacgtttt aggatgagta tcacagcatg acagcattgg gttgattagt tgaatcgatt
     7021 ggatggtaca agtttttttg ataaaaaaag gtcctgctag ccggggagct agcaggataa
     7081 ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg gtatgcttat ataatagtca
     7141 ctagttgtga atattttgtg acgaaagttt tcgaaagttg ttaagttttt ttgcgaaaac
     7201 tatttttata tcatatatat gtctaaaatc tatattataa aagaaatgtt gtttgatcag
     7261 cttctcttcg gtattagttc aaaacaaaac ggcaaagtat atggagctac tttgccgttt
     7321 tgtttctcta atactcaggt cagtttatag acgctcaata aacggtgttc aaaagccagt
     7381 ttctcggtat taagtcaaat ttatgcaaaa atgtgagaag ccattttcac aaaatatttc
     7441 ttaatacttg aaactaagcg gctttttcac gacctctcaa taaacggtgt tcaaaagtca
     7501 gtttctcggt attaagtcaa atttatgcaa aaatgtgaga agccattttc aaaaaatatt
     7561 tcttaatact cgaaactgag cggctttttc acgacctctc aataaacagt gttcaagaga
     7621 cagcctatcg gcaataaggc tgaacgtctc tttcacaacc tcttttatca atctgctact
     7681 tcttctttct tagcgaaact ccaaatcagt ccgataacaa aaccgataac tgctggtacg
     7741 atccacccca tccccaattt gaaaaacggc aagtagcttt ccgcaacata taaaagattt
     7801 tgaacaatag gtgtttgttt gatagtttct gggcaagcgt tcaacccatc aaaaatcgaa
     7861 gcaatcaatg taaaataagt agtcattcga taaacagccg tacgctgctt aaacaaagga
     7921 ccaactaaca ctagtaaaat cagtgtcatt gctaatggat agatgaacat caaaacaggc
     7981 aacgaaaatt gaatgatatt cgtcaagcct acgtttgccg ctaaacaagg aaggatacta
     8041 gcaagtgcaa caaagaatgc gtaatttctt tgcggaaaca aatctgtgaa tgtttctgca
     8101 aaagctgtaa tcaaaccaat accggtcttc aagcaagcta atataacgat aaatgctaat
     8161 aagatactgc cataagtacc taaataatga tcagcaatct gagccagcgt gatcccgcca
     8221 ttttcactta gagcaaagct acctaagctc atagcaccta aataagcgag caaagtataa
     8281 atgattccca tcaatataat gctgatcgca ccagatttga tcgtgtcttt agcgatttca
     8341 gcaggttttg ttacacccat gttttggatt gttgtgacaa tgatgatccc aaatgctaaa
     8401 gcagcaagcg catctaatgt gttatatcct tgagtaaacc ctgtgaaaaa agcatgctct
     8461 tgataatttg gctgaatagg cgcttgattt acagaaccta gtggattcaa aaaagcaagg
     8521 agaagcaaag cacctaataa aaccaaaaat gcaggattca ggaactttcc aacataatct
     8581 aatatctttg tcggttttct tgaaaaccac caagccagca caaaaaatac aatactgaaa
     8641 attgctagat atagcgtctg tctgctagga tcgataaatg gtgctaaacc aatctcgaaa
     8701 gatgtggttg caaggcgagg taaagcaaaa aacggtccga taactaagta aagcaagatg
     8761 gtaaaaacca tcgcatatgt ttgatttatc cgttttgcta attcatatac acctgagctt
     8821 ttcgatacac caatggcaat cacccctaag aagggaagac cgataccggt aaccaagaaa
     8881 cctagattcg cccaaaaggt agcagcgcct gcttcttgac ccaaatgaac gggaaagata
     8941 agatttccag caccaaaaaa tagaccgaaa agcatggagc ctataaaaag atagtcttta
     9001 aatgataatt tttttgtcat acataactcc tttacggttt ttttaaaagt aacaaaattt
     9061 cgaaaaatat gcaacaattt tcagaaaatt tcctatataa aacgacggtt atttaataac
     9121 gtaagtgata aaataaaagc gctgaatcat ctgaggagga atcatcgtga caaagtcgag
     9181 attagaagca tttactgatg gagtggtagc cattgttttg agagttcttg tattagagat
     9241 caagatccct aatacatcta gctttcattc tttatgggtc atccgaaata cattattagc
     9301 ttataccatc agctttatat ttgttgcggt gatctgggtg aaccatcatc gtatatttca
     9361 gatggcagaa cgaattaact accgagtggt ctggtcgaat atctttttgc tcttttggct
     9421 gactttatgt ccttctgtta ctagttgggt cgggcgtaac cctgaagctt tttggcctga
     9481 gatggcttat gtcttagtat acacgatgtg gagtttttct tttggtatct tgatgaggca
     9541 aatcatgaaa gcaaataaac cagattccca tgtcgtgaga gttttgtcaa aggaccgtcg
     9601 aagtcttatc tccatgatga ttaacttatg cttgattgcc ggtgtctttt ttgtaccgtt
     9661 gatcgggtta ttcggacgtt tttttgtatc tggtatttgg attttttcct ataaaaaagc
     9721 agatgcctat tatcagcggt tgtttccggg gaaatagaaa agaggctggg acagaagctt
     9781 ttaactccaa gaaataagcc gaaatttcac aaaaatttaa gaagcaattt tcgtaaattc
     9841 cttcttattt ctcgaagctg accacttctg tcacaactac tttcttactt cttacgttca
     9901 tatttcatat gatagattag taatagaagc aagaaccaca caggtgttag taataaagcg
     9961 attctcgtat cttttcctag gaacaagacg acacacacaa aggccatgta aagaaatacc
    10021 agataattac taaatgggta caacggcatt ttaaatgaat gagctttttt gccgaggata
    10081 cttttacgat atttcatgtg cgtgtagaca agcatactcc agataaacaa gaaacaagta
    10141 gtggcaatac tgcttactag tgtgaatact tcactaggca tcacgtaatt caagataaca
    10201 gaaatgaaaa tgacgatagt agaaaatgtc aaagcattcc ctggcacatg atgtgttgtt
    10261 aactttttga attttttagg ggcactgcct tcttgcgcta aagaacgaag catacgtcct
    10321 gtactatata ttgcactgtt gcaggcagaa gctgctgcac ttaatacgac taaattgatc
    10381 aaggaagcag caatcgtgat cccaatttcc gaaaatactt ctacaaatgg actgttatct
    10441 ggattcaagc tgttccatgg atagattgcc ataatgacaa aaagagatcc taagtaaaat
    10501 aagatgatcc tgataggaat attattgata gcttccggca acactttttc aggattttct
    10561 gtttctccag cgactaatcc aactaattct atcccagcaa aagcaaaggt taccatttgg
    10621 aaagctaaga taaatccttt tgcaccagtt ggaaagaatc cgccatgaga ccatagattc
    10681 gtgatactgg ctgtgccaat atctgtcgta tagtgggtga ggatcatata agctcctacg
    10741 aggataaagg caatgatcgc tacaactttg atcaatgcaa accaaaactc taactcacca
    10801 aacaatgaga cagccaccat attcaagcaa agaagaagac ctaatgcaat cagttcagga
    10861 agccactgag gaacaccagg cgcccagtag cgtacataca ttccgatggc tgttaaatcg
    10921 gccattgcaa tcgataccca gcagaaccaa tacgtccagc cagtgatgaa agccgctttt
    10981 ttgccaagat attctgcaac aaaatccaga aaagaatggt tattggtgtt tgataacaga
    11041 agttcgccta acgcacgcat aatgaaaaaa cagatgcatc cagtaataag ataggcgagc
    11101 aatatcgaag ggccagctag ctggatagac tttccggaac ctaaaaacaa ccccgttcca
    11161 atcgctccgc caatagaaat cagttggaca tgtctatttt tcagtccacg ctgcagttct
    11221 tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt ttaattactt ccatattaaa
    11281 agagttttct gaaaaacaca actaagattc caaaattttc agaaatttac cttttatgag
    11341 taattcataa aaaagaactg cggtaaacac cgcagctctt tgtcccaatc agctattgat
    11401 cctaagcatt taaaatacgt gaaaggaatt cttttgtacg ttcttctttc ggatggacaa
    11461 agatatcttc tggtgtacct tcttctgcaa tcacaccttt atccataaaa atgacacggt
    11521 cagatacatc ttttgcaaat tccatctcat gcgtcacgat aagcattgtc aagccagtat
    11581 gtgctaagtc tttgatcgta ttaagcactt cgccaaccat ttcagggtct aacgccgaag
    11641 taggttcgtc gaatagcaat acatctggat tcattgataa tgcccgagca attgcgacac
    11701 gctgtttctg tccaccagat aattgtgctg gtttggcttc gatataacga tccataccaa
    11761 ctttttccag attttcgatt gctacttttt tggcagtttc tttatctcgt ttcaggacag
    11821 ttgtttgacc agacatacag ttttccaaga cattcatatt gttgaacagg ttgaaggatt
    11881 ggaaaaccat acctagattc gttcgatatt taggtaaatt atatttagga gctaatacat
    11941 tttctccatt ataaatgatc tcgccgcctg ttggtttttc cagtaaattg atacagcgca
    12001 agaaagtaga tttaccagaa ccggaagaac cgatgatcgt cacaacttca cctttattga
    12061 ctgttacgct gatatctttc aatacttcat tttcaccgaa gctttttctt agatgtttga
    12121 tttcaataat tttttcattc atgattcttc ctccttattc ctgcaaattt gaattgtcga
    12181 tttcttcaag cttggtataa gctgaaggac catcgatttt cttttctacc gcccgcagga
    12241 ttctggtgat cgtaaaggtc atgactaagt agatgatccc gacgatcgta aatgtttgga
    12301 agaattggaa attcgcaccg gaagcagagt ttccttggaa gaacaaatca gctacgccga
    12361 ttacacttaa aactgctgta tctttgatat tgataacaaa ctcatttcct gttgctggca
    12421 agatattccg taaaacttgt ggaataacaa cttttctcat cgtttggcca tgggtcatac
    12481 caatggcttg cgcagcttcg aactgccctt catctactgc aaaaatcccc ccacgaacga
    12541 tctctgtcat gtaagctcca gtattgagag agacaataaa caaagcggca atcgttcggt
    12601 tcaatgaaat gccaaaagcg agtgccagac cgtagaagat gaccattgct tgtaccatca
    12661 tcggtgttcc gcggaatact tcgacataga tagaaagaag tgcatttccg attttttgga
    12721 agaagcgagt cagtgcattt cctgattcag gtaccgaacg gaagacacca atcagtaatc
    12781 caagtgtagt accgacaact gtcccgatca atgcgatgaa caaggtaagt ccagcacctc
    12841 gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa atcagaaatg atgccaggtt
    12901 tttcttcact atcagttgca gcaggctgat ctttgactgc ttggtccata attttggttc
    12961 gttcatcgtt agagatgcca gaaagaatct ggtttacttt ttggatatcc gggtcgcctt
    13021 tacgcatacc aacagaaatc tgaacatctt ccgggtttgt ctggaagcca tctgcaccgc
    13081 tgaattcaag catttccaag tctttgttca cactagttgc cgttacacct tccggacgtt
    13141 cgctgacata accatcgatc attccagaag ctaaagctgt tctcattgct gagaaattat
    13201 ccattgcttg ttgtttctgc acatctggta tctgatcgat cacagtatag tggaatgtat
    13261 ttaactgagc agtaatcttt gctccagaaa gatctgctaa actttttgct tttgcatatt
    13321 tgctatcttt ttgaactact actactaatt gtgattcgta ataaggatcg gtaaaatcaa
    13381 tttcttttcg tcgttcagca gtagggctca ttccggcgat gatcgcgtcg atcttgcctg
    13441 attgaagggc aggagccaga ccatcccatt tcgtttggac gatgactaat tttttcccta
    13501 aaccatctgc tactttttta gcgatttgaa catcataacc gccggcgtaa ctattttggc
    13561 cttgaattgg caaggcatta ttctcatctg tttgttgaga ccagttgaag ggtgcatacc
    13621 cagcctccat acctacgcga aactcatcgc tcggtgtttt ttcatctgca tttgccagag
    13681 atcccacact gaatagtgag aagacaaaag caaatagtac ggcaactgaa accatatttt
    13741 ttttcatttt tttctccctt tctgattata tgatacacga gcggtctaga tgaaatgcgg
    13801 taaagcttta tctgaaaaaa taaaaagcga ccgatttata gatataaaaa ggccgctaaa
    13861 attccataag gtgataaacc tctcacaaaa catagcgcaa cttagcatca ctgctaagac
    13921 agtccgcaag ctcttaactt gcgtcccaac atacactcca agcaaaaaag tgcacatttc
    13981 ggcgatcctc cctattcctg cttctccatg tttgcttcac tccacagggt taatgattct
    14041 cgcgacctct acctcattga ttgaggcatc tcgtattcag ttatttatac aaaaaaagtt
    14101 tacgatactc gagaggcttt gtcaatctat aactgctgtt ttttgaaaaa atactgataa
    14161 atatagaaat ctttactttt accgaaaaaa caatcaaaat gtgaatcatc tattcattgc
    14221 aaacgatagt ataattatac acaaaaatgt atgtaataca ataaaaaaag aactagaaca
    14281 gaaaatttcc tgtgtccagt tccatctcga tcatttaagc tgccgctttc tttggctcac
    14341 gtcctaggat cgcttgcaaa agaacaacga cgccataaat ggctaatatt agataaatca
    14401 aacttgagta ttgggtgatt ccccagcccc atgttcgata tacggctaat gtaatggcta
    14461 aaacaattgc ggcaatcacg ttgaataaag caaaagccca aagatttcca ttttctttgc
    14521 gtacaagata aaaaacagag aaataaacgc ttaacgctaa ccaggcgata caagccagaa
    14581 taacgcccca ataaataaag aatggtaaca tatcgattca cctcactttt atacagcagt
    14641 attgtaacac gttttcattc ttttgtcagt gaacattgtg ataaattgtt caaatgccca
    14701 taacactttt ttattcccat ttttctgact tttcaacaat aaatttgttc gagtatgggc
    14761 tataatggaa agtgaggtga caaaacatga aacaattaac agtagacgaa gcagcaaaag
    14821 agatattagc ttatcagcaa attacatttt tgaccggtgc aggggtatcc acaccttctg
    14881 gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg gatggagcag ccggaatact
    14941 tgctgagtca tcaagcaatg gtgaatgaac cggataaatt ctatcaattt gtcaaaaaga
    15001 tttatcatcc tgaagcaaaa cccaatatca ttcatttgga aatggcacat ctagcgaaag
    15061 aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt gcacgcaaag gctaaaagcc
    15121 cgcaactcgt caattttcat ggaagtttgt atcaatgtca ttgtcgaaaa tgtcaacaaa
    15181 cggtagactg gaaagaatat cttcacagcg acaaacatca agcatgcgga ggacaaatac
    15241 gcccagacat cgtgttatat ggtgaagggt ttcaagatgg agtcatggag caggcggctt
    15301 atgccgtcag ccaagcagag ctgatcgtca ttgttggaac gagttttcaa gttcatccgt
    15361 tttgtgatct ggtacagttt cgccagccgt cagccaaaat cttagtaatc aatcaaacgc
    15421 ctgtttattt agcggagcct tatacgttta ttcaagaaga cggagcggtt gttttcaaaa
    15481 aaatacaaga attaggagca gattatgact gaattcaaaa ccgaaaaaga aaaaatgatt
    15541 gcaggcgagc tttattttgc caatgatcct gagctggttg ccgaccgtat gtttgcaaga
    15601 agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac tgcgatccca attgttgaaa
    15661 gagacttttg gccgtacagg gaaaaaaatc tatatggagc cagttatcaa ttttgattac
    15721 ggatataata tatttgttgg ggagaatttt tatgcaaatt tcaattgtac atttttagat
    15781 gtcagcacga ttgaaatcgg tgataactgc atgttcgctc cgaatgtcca gctttacacg
    15841 gcgacgcatc cactacatcc agtgaaacgt aacagcggtc tggagtacgc aaaacccatt
    15901 aaaatcggta ataatgtttg gttaggcgga ggagttatcg tcacgccggg tgtcactcta
    15961 ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca aatcttttcc cgacaatgta
    16021 gtgattgcag gaaatcctgc tcgtatcatc aaggcagtag aggaagaact aacagaagag
    16081 tctcttgaaa cattacgtca taagatcgat atgatcgatc gagagttggt tgctttatta
    16141 gaaaaaagaa tggatacggt aactaaaata ggacaagtga aaaaagaaga aaaccaagca
    16201 gtttatgatg caaaaagaga gcagcaagta ttggataaag ttgttagtct gctgaaaaat
    16261 aagggatata aggaaacaat aacagatacg tatgttgatt tgatgaaaca ctcgcgcgaa
    16321 taccaaaaca aaatgaagga ggaataagtg tgatcgaagg agagacacgg cgaaaacaaa
    16381 tcatgcaaac cttggcaaat acagaaaaac cagtgagtgc tagcaagttt gcgaaaactt
    16441 ttggagtcag ccgtcagatc attgtaggcg atattgcttt actacgagct gcaggtaaaa
    16501 gtatcgtggc aacagcaaga ggttatatcc tcgaaacaga agaaaccaat actgggcaca
    16561 tcagtaaaat cgctgtccag catggaaaag atcaaacaga agaagaactg agactaatcg
    16621 ttgaaaatgg tggtgaaatc attgatgtaa ttgtggaaca tcccctttac ggtgagttag
    16681 caggaacttt gcacatcaaa acgccacaag atatcgattc ctttatgaga cgttatcaaa
    16741 aaagtaatgc tactttgtta tccgaattaa caggcggcat ccatctgcat acgatccgct
    16801 acccagaaaa gcatatgctg aaaaacatca aacaaaaact agctgaagca ggaatcctct
    16861 acgaaggata acatcgtagc tgctactttt tatcctagaa actattctat catctctgag
    16921 ataaacaccc atatggaaga aataagaaaa aataagaaaa gatgcgtatc caatacacag
    16981 aattttttgg aattgcgatt tctagatgtc tatctaaatt tcgattagtg gagaaacata
    17041 aaaaagggaa gtgcagcttt tgagcactcc ccttttttat aaagctttct tgtagcatag
    17101 tatcgaagga acctgccaat tctctactcg atcaccaata cgccttcttt tagtgcaaat
    17161 ggattttgat cgttgatgtg gtcatagaac atcacaccgt tgatatggtc gatttcatgt
    17221 tgaacgacga tcgattcata attcttcaaa cgaatcttgt gtttttcacc attcatatca
    17281 taataagaca cagtgatttt ggcatggcga acaacatatc ctggtacttc tcgatcaaca
    17341 gataaacagc cttcaccttc ccctaaacaa gcatcttgaa cagaatggct caaaatttta
    17401 ggattataca taacagtact tagagaaggt ccatctgcct ctgggtcagg acttggtacg
    17461 tgaacagcaa tgattctttt agaaatatct aattgaggag cagctaagcc aacaccgcct
    17521 cgtaagtgta gttcttcagc tttgattgga tcttggctgt tttttaagaa ctcaagcatc
    17581 tcttttccta aagagatatc ttcttcgctt aatggaagag aaacttcttt tgcaacttcg
    17641 cgtaaggtcg gatttccttc acgaatgata tcatccattg taatcatgga atatgtaccc
    17701 ctttcttaaa agactgtgtt tcaagtctat tttcgcatta tagtttacca caaaaatgga
    17761 aaagtagtag taagattgtt tgaaaactac cttttcttaa tgattaacta cttgattttc
    17821 aacccggttt caggtaaagt atagctgtgt gctctaggca catgacctgc cacttggtta
    17881 agcgaatcac caacgtttta ctcagtcgca ggggaataat cgaaagaggt gaaagaaatg
    17941 gcaatttcaa aagaacgcaa gaatgaaatc atcaacgaat acgctcgcca tgaaggggat
    18001 actggttctc cagaagtaca aatcgctgta ttaactgaag aaatcaacca cttgaatgaa
    18061 catgcacgcg tgcacaaaaa agaccatcat tcataccgcg gcctaatgaa aaaagttggt
    18121 caccgccgta acttgttagc ttacttacgt aaaactgacg ttcaacgtta ccgcgaattg
    18181 atcaaacgtt taggcttgcg tcgttaattt tagtcaaaaa gagtgggatt cacatgaatc
    18241 tcactttttt tactcgtata tcaattaagt aaaattccgt gatcaagcta gatggactcc
    18301 tactaaccaa atgaaagctg ctcaaaagac ggctttcggc agttttcatt tgtttagtag
    18361 cgtcttgctt tcacgagaag gaaaaggaga agttatgtca gaaaaacaag tattcaaaac
    18421 gacatggggc ggccgtccct tacaaatcga agtcggtcag ctagcaaaac aagcaaacgg
    18481 agccgtttta gtccgttatg gagatacagt agtcttaagt gcagctgtgg cctctaaaga
    18541 agcaaaagat acagactttt tcccattaac gatcaattat gaagaaaaaa tgtatgcagc
    18601 agggaaaatc cctggaggtt tcatcaaacg agaaggccgt ccaagtacgg aagcaacatt
    18661 gactgcccgt ttgatcgacc gtccaattcg cccgatgttt gcagaaggct tccgtaatga
    18721 agtccaagta acgaacatcg tgatgagtgt agaaacagac tgctcgccag ctatggcagc
    18781 aatgctaggt tcttctttag cactatcaat ctcagatatc ccatttgacg gcccaatcgc
    18841 aggcgtagaa gttggtcgtg taaatggcga gtacgtcttg aatccaacag tagaacaagc
    18901 agaacaaaca gatatcgaat tgactgttgc cggaacgaaa caagccatca atatggttga
    18961 aagcggcgcg aaagaagttt cagaagaaga catgcttggt gcattgctgt ttggttttga
    19021 cgctatcaaa gagttagttg ctttccaaga agaaatcgtt caagcagtag ggaaagaaaa
    19081 aatggaagtg actttactgc aagttgatga agtattgaaa aaagaaatct ttgatgcttc
    19141 ttacgctaca atgaaagcag cagtaatgac tgaagaaaaa cttgctcgcg aagataatat
    19201 cgagcaggtg aagatcgata tccgcgaagc ttatgctgag aaatttgcag gtcatgaaga
    19261 tgaagatcac ttgttgaaag aagttaaaca aatcacagaa gatcttgaaa aagatgtcgt
    19321 acgtgaattg atcaccatcg ataagatccg tcctgacggg cgtaaattgg atgaaatccg
    19381 tccattgtca tcagaggtca gcttattgcc acgtgtacat ggatcaggtt tgttcacacg
    19441 aggacaaaca caagctttat cagcttgtac actcgcacct ctaggagagc accaaatcat
    19501 tgatggttta ggggtggaag tcagcaaacg attcatccac cactacaatt tccctcaatt
    19561 ttctgttggt tctacaggac gtgcaggctc tccagggcgt cgggaaatcg gacatggcgc
    19621 attaggtgaa cgtgcgttag cacaagtaat cccttcagaa gaagaatttc cttatacgat
    19681 ccgtttagta gcagaagtat tggaatcaaa tggttcttct tcccaagcaa gtatctgtgc
    19741 tggcacatta gcattgatgg atgcaggtgt accaatcaaa gcgccagtcg ccggtattgc
    19801 gatgggactt gtttctgatg gtgaaaacta tacgatccta actgatatcc aagggctgga
    19861 agatcatctt ggcgatatgg actttaaagt tgccggtaca aaagatggta tcactgcttt
    19921 acagatggat atcaagattc aaggaatcac agaacaaatc ctaactgaag cattaactca
    19981 agcaaaacaa gcacgtatgg aaatcttaga agaattgact tcaacgattg ctgctccacg
    20041 tgaagaatta agccaatatg caccaaaaat cgaaatgatc cagatcgaac ctgctaaaat
    20101 caaagatgtt atcggtaaag gcggcgatac gatcaacgga atcatcgatg aaactggtgt
    20161 gaaaatcgat atcgatcaag atggaaaagt aagtattgct tcagctgatg cagaaatgat
    20221 caaaaaagct atcaagatca tcgaagattt gaccaaagaa gtcaaagttg gtgaagttta
    20281 tctagggaaa gttgtccgta tcgaaaaatt cggtgcattc gtcaacttga tcaaaggaaa
    20341 agatggactt gtccatattt cccaattagc aaacgatcgt gtcaataaag tcgaagatgt
    20401 tgttaaacta ggtgatgaga tccttgtgaa agtaactgaa atcgataaac aaggacgcgt
    20461 aaatctgtca agaaaagcaa tgttgaacga agatggttca acgaaaaatg aagaaaaata
    20521 atttaattca taggaataat gtacttgtga tcgtgaaggt gacaggggga caataattat
    20581 tcgaaaaaag gggactgtga caaaagccgt gtcacagtcc ttttttttcg aataaacggt
    20641 gttcaatcag ctgtccctcg atattagctc aaaaaacggc aaaatatgag gaactatttt
    20701 tccgtttgtt tctctaatac tcaggtcaaa ccgctgattt cacaacctct gattcacggt
    20761 gttccagaag cagcttctac ggaaataagc tgaacacttt tgttacaact tctttttttc
    20821 tggagtttct tggagcatat ttttggagtc cttctgaata aagcgtaaaa taaaagtgag
    20881 aaatatggat aaaggaggga aaggagatgc tgaatttttt atggtcgtta tttatcggcg
    20941 gtatattagg tttgatagct ggcgcaattt taggaaagga agtcccggga ggcgtatctg
    21001 gaaatatcat tatcggtttt ttaggtagtt ggctcggcga atttttacta ggaccactgg
    21061 gaccggaact cggcggtttt tatttgatac cagcattatt gggtgcaatt ttttgccttg
    21121 ctctttattc atttgtggca agacgtatca gagaacgtcc aaagaaagaa taattttatt
    21181 caaataagtt gacacttcag tgtgttactt ggataattag aagcaattta ggaaagcggg
    21241 tgtcatggat aagaatatca tctatcattc aagcttacct aaaggatatc catataacaa
    21301 gaaatgaggg aaaaacgtgg caacaacgca caataccaaa aagatatcaa tggctggcct
    21361 gcttgtagcg atgggagtcg tctacggaga catcgggaca agtccgcttt acgttatgaa
    21421 atcgattgtt gaagataatg gcggattgtc aggaatcaat cctgattttg ttatcggatc
    21481 agtctcattg attttttgga cattgactct actgacaaca atcaaatatg tcgtgattgc
    21541 attgaatgca gataaccatg gtgaaggtgg aattttctca ctctacacac ttgtccgaaa
    21601 aggtgggaaa tatttgatca ttcctgcaat gatcggagga gctgccttac tagcagatgg
    21661 ggtattgacc cctgctgtga cggtaacgac tgcgattgaa ggcttgcgag gaattccgca
    21721 gttttttgac cggttcggta ataaccagaa cgtgatcgtc attatcactt tagttattat
    21781 ttttgtttta ttcatggtcc aacgctttgg tacagatatt gtcggaaaag cattcggacc
    21841 aatcatgttc gcttggttta cttttttagg cgtaatggga ttaatgaact tcagtcaaga
    21901 ctggacagtt atccgtgcac tgaatcctta ttatgctatc cagctgcttt ttagtgcaga
    21961 taacaaatta ggcttgttca ttcttggaaa tattttcctg gctacgacag gtgcagaggc
    22021 gctttattcc gatcttggac atgtcggcaa acgaaatatt cgtctgagct ggccttacgt
    22081 caaaatttgt ttggttatca attattttgg gcaggctgcg tggttgttga atgtttatca
    22141 aaaccctgaa gcacagcaaa tcaagaacct gaatccattc ttccaaatga tgcctcaaag
    22201 ttggatcgtg atcggcgttg cttttgcaac agtcgctgca gtcattgctt cgcaagcatt
    22261 gattacaggt tcgtttacgc tagtttcaga agcaatcaag ttgaaactgc tgcctcggtt
    22321 aaagatcatg tatcctggga acagtatcgg gcaaatgtat atcccagcag tcaatctgat
    22381 tttatggatc gcctgttctc tagtggttgt cacattccga acgtctcatc atatggaagc
    22441 tgcttacggt ctatcgatca ctgtgacgat gttgatgaca acagcactgc tttactttta
    22501 tcttatccaa aatgggcatt ctcgttggct tgcatattta gttacctttt tcttcggtgc
    22561 gatagaagtc gttttcttta tctcaagtgt tgtcaaattc ttccgaggcg gttttgtagc
    22621 cgttctgatt ggtttgatca tcttagtcgt gatgtttatt tgggaacaag ggaacattat
    22681 tcgcgaatcg tcaaccgagg atgtgtcttt gagagattat atcccgcagc ttagagaatt
    22741 acgtgatgat acttctttac caatctatca gacaaatgtg gtcttcttgg ttcctgatat
    22801 ggctgaagat aaagttggcc gacagttcat ctattcgatt ttagataaac ggcctaaaag
    22861 agcacgagtc tattggtttg ttcatgtgga agtgacggat gagccatata cgaaagaata
    22921 tcaagtagat atgatggaca ctgattttat tgtccaagtg aatctgttct taggcttcag
    22981 ggtccaacag gaaatcaacg tatatgttcg ccaagtcatc catgatttga tgaaacaagg
    23041 ccgtttgcca aaacagccac aaagatactc tctgacacca gggcgagaag ttggcgattt
    23101 ccaatttgtc ctgattgatg aagtagtttc caatgtgaca actctaggaa aatggcaacg
    23161 tcaaatcatg caggcaaaac tggcaatcaa aaaaatcgct acctcgccag aaacatggtt
    23221 tgggctagag tacagcgaag taaaacatga aactgttccg cttatcattg gaaatactcg
    23281 gaaaacatgg ttgaaagagc ggttgtgaaa gtagcgcttt gacctgaact aatgtcgaat
    23341 gtcagctact tgaacaccgt ttatcagagg tcatgaaaaa gctgtactgc aacaaacagt
    23401 aagaacgagc agataaaaac agcttctcat gtttttcatc tgtttgggct taatgccgca
    23461 gatgcaagct tttgaatacc gtttttatca gaggtcatga aaaagctgtt tagcaacaag
    23521 taacgcaatg attttgaagt gataaggaga tgtggctgaa aaatgccgca tctccttttt
    23581 tattcctaaa tatgaatacg cattattttc aatctttttg tagaaaaagg gtacaataga
    23641 aaaagagaaa ggcgggagaa tatggttatc aatttcatta ttttattttt gatgattgca
    23701 attacttcat tttttgtagc aagtgaattt gctttagtga aaatcagacg atcaagattg
    23761 gaacaactag aaaaggaaaa tgtaaagaac gccaagctag ctcttcatgt tacacaccat
    23821 ttagataact atttatcagc tagtcaatta ggaattacgc taaccggttt gatcatcggg
    23881 tgggtcggtg aaggatctgt cgcggcttta ctagaaccag tgatcggtaa actacctttt
    23941 agttcggcta ttagcagtac aatctctgtg gcgctgggat ttattttagt tacgtatatc
    24001 gatgtggtta ttggcgaact gcttcctaag agctatagta tcgtcaatac ggagaaggtc
    24061 gtcttgttcg tagttaaacc acttcattat ttttataaag taatgtttcc ttttatttgg
    24121 gtattgaatc attcagctgc agggctaggg aagctattag gtgtgcgtct cgtatctgaa
    24181 ggagaagaga cattgtctca agaagagctg acattagtcg cattgaattc ttatgaaaaa
    24241 ggagagttga ccaaagaaga atatcattat ttggaaaacg tgtttgaatt tgatgatacc
    24301 ttggcaaaag atatacaagt agaccgtacg tccatgcaag tattcgaagc ttcagaaacc
    24361 gtcaaagaag cgataaaagc ttcgattgaa caaggacata ctcgctatcc ggttatctta
    24421 gaatcaaaag acaatgtttt aggttatgtc accttgccag atttgatcaa gcaatcttat
    24481 aaagatgacc agctgacagt agaacagttg attgaagagc cgatcgtaac aactgaaacg
    24541 atccctataa aaaaattatt gacgatcatg cgaaaaaagg gaaagcatat tgctatctta
    24601 aaagatgaat acggagggac aagcggctta gtgacgattg aagatatttt agaagaaatc
    24661 gtcggggaaa tccgagatga gacagatttg gacgaagcat tgatagcgga acagtcagat
    24721 ggctcttata ttatctccgg caaactaaca ttagatgatt tccagcgtta ttttcatgta
    24781 gagattccag agtttgaaga aacgaatttt actacacttg cgggctttgc ttctagccga
    24841 tataaagaaa taaaagcagg aacaatcatt gagattgcat cattccgttt cacggtatta
    24901 gaataccagc atgcacatat cgattatttc aaagtagagt ccacggaaag aaaaacagaa
    24961 taaaagaaaa aaggctgtga cattttttgt cacagccttt ttcctacgat tagtcaagta
    25021 aaaggtgcaa gaaaaatagc tcgtaaacga aagcgacaaa atcaaagcac accaaataaa
    25081 gccccgttcg taaaaaattt tcgatctggg caaagaagtg gtatactgta agtggtttag
    25141 ttaatggtcc actgagaccg cgtacgcata gtcgtactct gtatgtgccc taaccatagc
    25201 gtgcatacat ttcagaagct tgttcataca agcaactgtg gcaaccttat c
//
LOCUS       DACEUF010000040        24864 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.041, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000040 DACEUF010000000
VERSION     DACEUF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.041"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            129..1388
                     /locus_tag="IXG20_001533"
     CDS             129..1388
                     /locus_tag="IXG20_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ1609735.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(1477..2424)
                     /locus_tag="IXG20_001534"
     CDS             complement(1477..2424)
                     /locus_tag="IXG20_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1609736.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            2818..4110
                     /locus_tag="IXG20_001535"
     CDS             2818..4110
                     /locus_tag="IXG20_001535"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ1609737.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4125..5894
                     /gene="aspS"
                     /locus_tag="IXG20_001536"
     CDS             4125..5894
                     /gene="aspS"
                     /locus_tag="IXG20_001536"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ1609738.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            6003..6878
                     /locus_tag="IXG20_001537"
     CDS             6003..6878
                     /locus_tag="IXG20_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1609739.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            7144..9027
                     /locus_tag="IXG20_001538"
     CDS             7144..9027
                     /locus_tag="IXG20_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1609740.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(9102..9386)
                     /locus_tag="IXG20_001539"
     CDS             complement(9102..9386)
                     /locus_tag="IXG20_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1609741.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(9376..10110)
                     /locus_tag="IXG20_001540"
     CDS             complement(9376..10110)
                     /locus_tag="IXG20_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ1609742.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            10239..10862
                     /locus_tag="IXG20_001541"
     CDS             10239..10862
                     /locus_tag="IXG20_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ1609743.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            11054..12175
                     /locus_tag="IXG20_001542"
     CDS             11054..12175
                     /locus_tag="IXG20_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ1609744.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            12182..15310
                     /locus_tag="IXG20_001543"
     CDS             12182..15310
                     /locus_tag="IXG20_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ1609745.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            15329..18523
                     /locus_tag="IXG20_001544"
     CDS             15329..18523
                     /locus_tag="IXG20_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1609746.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(18669..19376)
                     /locus_tag="IXG20_001545"
     CDS             complement(18669..19376)
                     /locus_tag="IXG20_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1609747.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            20141..20539
                     /gene="spxA"
                     /locus_tag="IXG20_001546"
     CDS             20141..20539
                     /gene="spxA"
                     /locus_tag="IXG20_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ1609748.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            20871..21524
                     /locus_tag="IXG20_001547"
     CDS             20871..21524
                     /locus_tag="IXG20_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ1609749.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            21750..22748
                     /locus_tag="IXG20_001548"
     CDS             21750..22748
                     /locus_tag="IXG20_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ1609750.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            22924..24732
                     /gene="pepF"
                     /locus_tag="IXG20_001549"
     CDS             22924..24732
                     /gene="pepF"
                     /locus_tag="IXG20_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ1609751.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT         8356 a         4259 c         5213 g         7036 t
ORIGIN      
        1 ttaagttaga ctgataaact ctaacttaag tggctttttt gtctgcaaaa agcgcttctt
       61 ttgtcagcag tcggaaaaaa agttaagctt aaattataca agttattttt tataggaggt
      121 tgctgaagat gaaagaagta ccagtagtag atatcaaaat cacagattta aaaaaattgt
      181 atcaaaaagg atacaacatg ttggaggagt tacgccatga agcggacgct cccgtcgtca
      241 aagcaaagat tttcaataaa gaagctatca cgatctacgg atcatcagca gcaaaagtat
      301 tttatgatcc aaggaatttc aaacgaaaag gtgctatgcc aaaattagta ttgaagacac
      361 tttttggtca aggtggagta cagactttgg atggagctgc tcatcaccat cggaaaaata
      421 tttttatgga tctaatgaca ccggaaagaa tggaagatta tcatcgtatc ttagataaga
      481 atctgacaca agcacttgaa gcacagcatg gtcaatttga actatttgat ttaagtaaga
      541 tggttttctt tacatctatt tgcgagtggg caggtatcaa tttatcagcg atttctaaag
      601 atgaggtcga aaaattagca gaatatcaaa tctctatgat cagcggaacg tttacttctc
      661 cgattgatca cataaaaggt gtcgaaaatc gcaaaaaatc agaaaaatgg gcgcaaggct
      721 tgattgaaga agctcgacaa aatcctgtgg ctggaaaaga aaatgtggca ttatatgctt
      781 ttgccaatgc cacagattta gatggtcagc ttttacctct ggaagtggca gctgttgaat
      841 tattgaacat catccgtcca acggttgctc taactgtgtg ggcggcattg atgggacatg
      901 cacttttcag tagaccagat ttgtatcagc aattaaaaaa tgacttttcg acgttacaag
      961 atccgtttat tcaagaaatg agacgctatt atcctttctt cccaatgcta cctgcgattt
     1021 cgctgaaaga agtagaagta gatggttatc gtataccaga aggcagttgg gttattttag
     1081 atttatatgg aacagaccat gatgaacgaa cagttgaagc gccagattcc tttatgatca
     1141 aacggtatgt agggaaagca aaagatattt cctataaaga agaatatgag atgattgcac
     1201 aaggcggggg aaattttcga caaatgcatc gctgtgcagg agaatggatc actcttcata
     1261 gtctacgtgt gttttccgat cagctagtaa ataagtttga gttctctgta cctgaacaag
     1321 attggacgat tccattcaat cagtttccga cttatccgaa tagccgagcg ctactttaca
     1381 agaattagat tagcataaac ttatcgaaaa aaaggaagat caaacagtgc tgatcttcct
     1441 tttttcatat tactataaat tgataaggtc aattgatcaa aatccacgaa atgcttcatc
     1501 aatggtttga taatcttcat tggataaatg caaatctaaa gcttggacat tgtcagatac
     1561 ttgctctgga cgcttagctc caggtataac tacagacact cgtggattct tcatgtacca
     1621 agctaaaaca agttgcgcag gagtggcttg gtatctttta gcaatggggc gaagtacatc
     1681 gacagcggtt aaaatttctc tgaaacgttc tccttggaaa tctggatttc tttttcgagg
     1741 atctccttca ccaaattgtt tttcctcacc aagttcatat tttcctgtca gcaaaccgga
     1801 agcaagagga aaatagggaa caaaactgat tttgtttttt tcaagataag ggaataattc
     1861 tttttcagcc tgccgatgga tcaaactgta tttatcttct actacatcga catatccatc
     1921 tgcattcgct tctttcaatt gttctaacgt gaagtttgac acaccgactg ctcggatttt
     1981 tcctgcttct tttaattcgt gcaatgtagc aactgattca ttttttggtg tgctttcatc
     2041 cggaaagtgg atataaaaaa tgtcgatgta atcagtttgc agacgtttta gtgcatcttc
     2101 caccgattgt ttgagaaact caggagaatt atcaaatgtt cgccctttat ttggtacatg
     2161 agcagctttt gtagcgatga cgacacgtga gcgatcatat tctttttcct tcagtacctc
     2221 gccaatcagt tcttcggaac ggccgtttcc gtaagcataa gcggtatcga tcaaattgat
     2281 cccgctattc aaagctgtac gaactacttg ttttcctgtc tcatcttcca aaccgtcaaa
     2341 aagattatgt cctccaacgg catttgctcc taatccaagc tgttctgcgt agacttgtga
     2401 atgtccgatt cttatttctg ccatagctga ttcctccttt ttgtctagtc taaaacaaaa
     2461 taccaaaaag ctgaactaaa acgtttttgt gaggggaaat agtcagatat ctcttgactt
     2521 tgagactggt ttttagtagg ttataagtaa tatcgaatgt caatgaaaga gcgagtaagt
     2581 ttttgaaact ttacaagaga ggatcatcga ggctgaaaat gatctaaagt ttcattagcc
     2641 taaagacact ctggagatgg acgagtgaaa agccgtcccg cgtacgagcg ttattcgtaa
     2701 gaggaaagag aaatctttca aaattaggtg gtaccgtgta tgaatttcat tcacccttgt
     2761 tcatgatgaa caagggtgat ttttttattt acatagctaa gaaagggaga gaaaactatg
     2821 agtttccaaa gaccaaaagg aacaaatgat attttgccag gcgaatcaga aaaatggcag
     2881 tttgtcgaag aaacagcacg tttattattc agagattatc agtataatga aatgcgtaca
     2941 ccgatttttg aacactatga agtgatatcc cgaagtgtag gggatacaac ggatatcgtt
     3001 tcaaaagaga tgtatgattt ttatgacaaa ggggaacgcc atgtgacgct tcgaccagaa
     3061 ggtacagcac caatcgttcg ttcttttgtt gagcataaat tgttcggccc agagtatgcg
     3121 aaaccgttca aaacttttta tatgggacca atgtttcgct acgaacgccc tcaaaaagga
     3181 cggttacgtc aattccatca aatcggagta gaagcatttg gatcagaaaa ccctgcaact
     3241 gatgtggaga gcatggtgat ggcacttgat ttcttcaaac agttggggat cacgcaaatc
     3301 cggctagtca tcaattcatt aggtgataaa gaaacgcgtc aagcctatcg tcaagcattg
     3361 atcgactatc ttttaccttt tgaatcagag ctgagtgaag actctaaacg ccgccttcat
     3421 gaaaatccat tacgcgtatt ggacagtaaa gacaaacgag atcaaaaatt tgtggcagaa
     3481 gctccatcta tcttagatta tctaagcgag cctgctcaaa aacattttga taccgttgtt
     3541 tccatgctgg atgcattggc tgttccttat gaaatcgaca gtaatatggt tcgcggacta
     3601 gattattaca cacatacgat ttttgaaatc atgagtgacg caccaaaaat gggggcacaa
     3661 gcaacgattt gcgcaggcgg acgttatgac aatctagtgg aagaactagg tggtccttca
     3721 acacctggct tcggatttgc aatgggtatc gagcgattac tcttgactat ggaggcagag
     3781 gaagtagtta tccctgcatt taatgagttg gatgcttacg tggtcgcact cggagacgaa
     3841 acaaatatcg aagcattgaa agtggtccaa gcgattcgaa actttggttt ttcagctgac
     3901 cgcgacttca tgaaccgtaa agcaaaagcg caatttaaga cagcagataa agcgaatgcc
     3961 aaattagtat tggtgatcgg tgaagatgag cttgctagtc aaacgattaa tgtcaaagca
     4021 atggcaagcc gaaaagaaaa aagttttcca ttagaagaga tatatgaaca ttttgatgaa
     4081 gtctatgatg aaatgacttt attcgagtag gaggaaaaaa ataaatggca caaagaacga
     4141 tctattgcgg attagtttcc gaacaacaat tagaacaaac agttacatta aaaggatggg
     4201 tacaaaaaag acgagacctt gggggagtaa tctttatcga cctacgtgat cgtgaaggaa
     4261 tcgttcaaat cgtctttaat cctgaaatca acaaagaagc atgggaaatc gcggataaat
     4321 gtcgcagtga atacgtgatc gaagtgactg gggaagttag aagacgcgga gaaaatgcag
     4381 taaatcctaa aatggccact ggtgaattcg aagtgattgc aagtgaaatc gtgattttga
     4441 atcaagcaaa aaccccgcca tttcaaattg aagatgatca agcaatcagt gacgaaatac
     4501 gtttgaaata tcgttatttg gatctgcgtc gtccaaaaat gactaaaaac atcaaattaa
     4561 gaaatgcgac aacaaaagcg attcgtcatt atttagatga taatgacttt ctcgatatcg
     4621 aaacacctta cctagggaaa tctacaccag aaggcgcaag agactaccta gttccttcac
     4681 gtgtccatgc tggacatttc tatgcgttgc cgcaatctcc tcaattgttt aaacaattgc
     4741 tgatgaatgc tggattcgac cgttattatc aaatcgttcg ttgtttccgc gatgaagatt
     4801 tacgtggaga ccgtcagcct gaatttacgc aagtagatat tgaaacaacg ttcttaacag
     4861 cagaagaaat ccaaacttac acagagggat tgattgcgaa ggtaatgaaa gaagttcgcg
     4921 gtatcgaagt cacattgcca tttcctcgta tgacctatga tgaagcaatg gctcgctacg
     4981 gcagcgacaa accggataca cgttttgata tggaattgat cgatctaagc gatacagtca
     5041 aagaagtaga attcaaagtc ttccaaatgg ctcttgaaaa cggcggtgtc gtgaaagcat
     5101 tgaatgctaa aggcgctgca gatcgctact cacgaaaaga catggatcag ttaggtcaat
     5161 atgtaggaca attcggagct aaggggcttg catggctgaa agtagaagaa gacggattga
     5221 aaggtccaat tgctaaattc atgggagaag caacagaagc aatcattaaa gcaacagatg
     5281 caaaaccagg tgacttgctg atgtttggcg cagataaaag tgaaatcgtc gctgctgcat
     5341 taggtgctat ccggacacgt ttaggaaaag aattaggact aattgatgaa tcgaaattca
     5401 atttcttatg ggtaactgat tggccacaat ttgaattttc agaagaagaa ggtcgatatg
     5461 tctctgctca ccatccattc acgatgccaa aagaagaaga tatcccgtta ttggctgaag
     5521 accctgcaaa agtctatgca gaagcttatg atatcgtctt gaacggttat gaactaggcg
     5581 gaggttctat ccgtattcat actcgtgagc ttcaagaaaa agtactagaa actctaggct
     5641 ttacaaaaga acaaatggaa gaacaatttg gtttcttgtt aacagcttta gattacggat
     5701 tccctccaca tggcggtatt gcattaggat tggaccgttt tgcaatgtta ttagccggag
     5761 aagataatat tcgtgaagtc attgcctttc ctaaaaacgg aaaagcagca gatgtgatga
     5821 gtgatgcacc aagtatggtt tcgcctttac aattgttcga attgaatatt gatgtaacga
     5881 atatagaaga atagccgata aaaaggtaaa aaggatttgt cgaatacgct tcttttgaca
     5941 accttgattt aattgaaaaa cactttataa tgaagccata acatataaag cggggctttt
     6001 ccatgaaaaa aacattcgaa aattgggctt atcatgatta cacgaccaag ctttcggtat
     6061 ctatcgttta tgcgttgatc gcatcagtcg cattgaattt cttttatcag ccaggacata
     6121 tttattcaag tggtattact ggtctagctc agattttgac gactttgtca aaatcactga
     6181 taggattcga agtaccggta tcaacaactt tgtatttact gaatatacct ttgtttgtat
     6241 tggcgtggtt aaaaatcggg aagaagttta caattttcac ttttatcaca gtcactttga
     6301 cctccctttg tatccatatt ttaccggaaa cagtcctatc taccgatcca atcatctgtg
     6361 cgttgtttgg gggggctgtg atgggactag gtattgggct agcgttgaga aacggcctat
     6421 catcgggcgg tctagatatc gtgagtatca cgattcgtaa aaaaacagga aagtcgattg
     6481 gttctatttc gatctacttc aatgcattga tcgtttttgt tgccggaatt ctatttggct
     6541 ggcaatatat gttttacagt acgttgtcta ttttcgtcag tggaaaagta actgatgtcg
     6601 tttatactaa acaaaagaag atgcaggtaa tgatcgtaac gaaacatcca gaaaaagtag
     6661 taaatgaaat ccaaaactgt atgcgtagag ggatcacgat catcaatgaa gcagaaggtg
     6721 cttataagca tgataagcag acagtattga tgacgatcgt tacacgatac gaactgcctc
     6781 tacttagaga agcaatgaaa gcttctgatc cacaagcttt tgtaagtatt gcagaaaacg
     6841 tccagatact tggtcgtttc tatgaggatg gcatttagat cgagtttgac aaaaattcat
     6901 gaaatactgc tgtagacaga cgttcgtttg tctgcagttt ttttctacca tagtagaatc
     6961 agcaaagaaa aactgctttt ttatttgaaa ttttcttaaa gttgaaaaaa agcatgttta
     7021 tttttttaaa tttatgggat gatagaaaaa aatagtagag aaaggacacg attctacttg
     7081 ctattttcac ttaatacccg tatattacga atgaaaaaag tttatgaaaa gttggaatat
     7141 tgaatggaaa aacaaaaaag gttaaaaaac agccgctaca tcactgggtt tgatgggctc
     7201 cgttcattag ctgtggtggg tgttatcttc tatcaccttc tgccaactag tatgaaaggc
     7261 ggctatttgg gcgttccgat ttttttcgtc gtatctggtt atttgatcac ggatctgctg
     7321 cggcaggaat gggaacaaaa tggaaaaatc aatatttggc aattttatgt cagaagaatg
     7381 aagagactct atccggcact tgtttttatg cttgtgacag cttcggctta tatcactttg
     7441 tttcagcgag ggctgttaaa caatcttcga ggagttgtgg tcagcagctt actgtatgtc
     7501 aacaactggt ggcaaatcaa taacggttta tcttatttcg atcgatttgc taatgagtca
     7561 ccgtttacgc atatctggtc tttagcagta gaaggtcaaa actatttggt ttggccgatc
     7621 ttgtttgtat tgttgatggt atttgttcgt aaaagaaaat ggatctttta tactgtgtta
     7681 ggtacatcct tcctctccgc catcttgatg atgatcttgt atacaccagg aggcgatcca
     7741 acccgtgttt attacggtac cgacactcgt ctgttttcta tatggttggg aagtgcgctt
     7801 gcgtttgttt ggccaagtac gcgtttaaag aaaaacatcc cgaaacaagc gaaaaatatt
     7861 ctgaatatcg ctggaggaat ctcgcttttg gcactgattc tttgcttctt ctttttagat
     7921 gatcattaca atttcatcta ttacggagga atgctcctag tgagtctgtt atgcgtagtg
     7981 ctagttgcag tgacagcaca ccccggagca agtttgaatc gatggctgac aaatccagta
     8041 tttacttgga ttggcaaacg aagctatggc atttatctgt atcagtttcc agttatgatt
     8101 ttttatgaag caaaggtgac tgatattgcc gatcatgttt tgttgcatac tttagtggaa
     8161 atcacattga ttctaatcat cagtgaactt tcttatcgat atgtggagcg tccgctagca
     8221 cgttttgatt atggtcgtac attccaagtg atgaaaggat ggttcagcaa accgatcatc
     8281 agtaagagca aaccatgggt tgttccaggc acattacttg tagcaattgc actcgttggt
     8341 ttcgttacgg caccgaaaaa cgctgtaaca gccgatcaaa aacaactgca ggcaaaaatc
     8401 gctgaaagta aaaaattagc agaagaaacc agaaacaatt cagaaaacac cactagttcg
     8461 gttgaccaag cgatccttga taagtacgag ttgtccaagg cacaagggaa taaggcacaa
     8521 aaattagaac taacggcatt tggtgattca gttatgttgg atgctgcaac ggatctacaa
     8581 gaactttatc caaaagtagt ggtagatgga gatgtcggac gacaattata tgccagtgta
     8641 ccgtacattg aaaaattaaa ggaacaagat ttgttgaaag acactgttct gatcggacta
     8701 ggaacgaatg gctcattctc ggaagctcag tttgatagtg tgatggatgc aatcggtgat
     8761 cgaaaagtat actgggtcaa tgtccgagta ccgacgaagc gctggcaaaa tgacgtgaat
     8821 gcgatgcttg aacagatgac gaaaaaatac gacaatatga cgctgattga ttggtacagc
     8881 tacagtaatg accatagtga ctggttttat gatgatcaag tccatccaaa tcctgaaggg
     8941 atgacacatt acattcactt agtcagcgaa gccttgctag gaggaagttc aagtacgaac
     9001 agcagtgaga caaacgtgac agaatgatag ctacaaaaaa cttcaggcaa aaacatttgt
     9061 ttttgcctga agttttttta tatttaccgt gtttttaaca ttcatataga ttcaggaagc
     9121 aaaacggttg agtgtgtctg taaataacgt tctttttgtt ttcgggtgag ttttttgaat
     9181 gcagcttctg cttttgttgc ttcgcttctt gaagaaaata cttctgcatg gatcatctta
     9241 acaggtcttc ttttttgaag acgagtatat ttagcaccag taccattgtt atgttcctct
     9301 aatcgtctag aaagatcggt tgtatatcca ccataaaatg tctgatcttt acagaataat
     9361 acgtaaaaat aatgttcatt tgccatagag catctcacga atctctgcag tgtattctcc
     9421 atccatattg taagtgatca atggtggagc gactttgaat ccgtctaatt tgccttcttt
     9481 gattccttca actaacaaaa tatttgcctc ttttccaggt tttgggtaaa caaattggat
     9541 ccttttaggt gcaattcgat agtcttccat gacctgaatg atttctaaga accgatcagg
     9601 acgatggacc atagtaaagc gtccatttgt ttttagtagc ttacttgaaa tatcgatcac
     9661 ttcttctaac gtcgtatgaa tctcatgacg ggcgattgcc aggtgtttat tagggttcgt
     9721 tttatttgtt ggcagccctt tgaaataagg tggattacag acgactaagt cacaagaatt
     9781 atgtcgaacg acagataagc tgtctttcag atcgatggtg tggacagtca tttgttcatt
     9841 taaatggtta agcgcgatac tacgtcttgc catgtctgct agacgaggtt gaagttcgat
     9901 agcgtcgata gccgcctggg ttcgtttact taaaaaaagt ccaactgcgc catttcctgc
     9961 acaaaggtca acaatcttcc cttttttagg tacacgggaa aagtgagcaa gcagcacagc
    10021 atctaaagaa aaagaaaaca ctttggaact ttggataatc ttgatatctt ctgcgtataa
    10081 ttggtcgatt cgttcgtctt cgtgaagcat ggattccctc caaaatgaaa aaaatagtcg
    10141 tctgtattat actattttta ttaaaaagaa ccaaggtggc ttgtcaaacc tctatttttt
    10201 atgcatacta tgaccataag aagggaaagg aagagactat gttttatcga tttatgcgcg
    10261 gggtagttcg agtactatta gccttagtta atggaaatgc ccgctatgag aataaagaag
    10321 ttttaccgca agatgagaat tacatactag ttgctcccca tcgtacttgg tgggacccat
    10381 tatatctggc ggtagctgca gcacccaagg aattcagctt tatggcaaaa gaagaactgt
    10441 tcaaaaatcc agttctgcga tttatattaa ctcgatccaa tgcttttcca gttaaaagag
    10501 aaaatccagg accaagcgtc attaaaacac ctgtaaagat tttgaaatcg acaaatttga
    10561 gtttgatcat gtttcccagt gggacaagac atgctacaga acttaaaggc ggaatggcat
    10621 tgatttccag aatgtctaaa gcgaaaatcg tgccagctgt atatcaaggt ccgttgacac
    10681 ttggggatct gttcaagcgt cagagagtga cagtgcgttt cggcgaacca atcgacttat
    10741 cagatatcaa gaaaacggat aaagaaggta tggcggagat cgaacgacga acacaagcag
    10801 cttttgacaa attggacaaa gaagtgaacc ctgatttcaa atatgaagtc aaaaaaaaat
    10861 aaaatgataa gtttatgcgt gggcggtctt ttgaatctac tgtcctgttc agttcagact
    10921 ttttcaatga aaacgaggga gagacgtcaa gtctgccctc tttttgcgtt ccaatatgta
    10981 aaatagaaga caggtgtcat gtaatggatc aaagtgttat aataattaga aggacatttt
    11041 ttgaggtgat tgaatgcgtt ttttacatac agccgattgg catatcggaa aaaaactaca
    11101 aggttatgac ttgattgaag accagcgtca tgtactatct tctatactag atatcgcgaa
    11161 agaagaaaaa gtagaggcaa tcgtgattgc cggagactta tatgatagga gtgtcccagc
    11221 tgttgatgcc gtagaattat acaatgaatt gatggtcgat tggaatttaa aggaaaagat
    11281 cccgattttt gctatatcag gaaatcacga tagcagtacg agactttcag cgggtacgcc
    11341 atggttcagt tattcgaact attttttgcg aacacagcta gcagaagcat ttgaaccagt
    11401 cgagtttaaa aacgtacaat ttttctttct tccttacttt gaaccaattg cagcgagatt
    11461 atattttgaa gatgacaata tccgaacgat caagcaagcg gtagccaagg tgatagataa
    11521 aatgcaggag ctatttgatt cagaaaaaaa acaagtattg attagccatt tttttgtaat
    11581 gggaagtgta aaatctgatt cggaaacgaa aatcgaagta ggcgggttgg attcgatccc
    11641 tggtgaacta cttacaccgt ttgattatgt agcactagga catttgcata atcaagctgc
    11701 tttgagacag gataatgctc gctatagcgg ttccccattg aaattctctt tatctgagat
    11761 caaccaaaaa aaaggcgtat ggatcgttga cctgacaaca ggcctggagt tgaattttag
    11821 ggagatcgaa cccctgcatg atattgaaga aataacagcg agtttcaaag aattgctgga
    11881 tcctgccttt tatcagtccg tcaatcggga gaattacatc tatatctatc tcactgatag
    11941 aacgatcatc ccgaatatga tgaaccagct tcggcaaata tatcctcgta ttttggggat
    12001 tgaacgggca aacggtcgag cagaacaaac aaagcgcaaa cgaaaagtac tagaaatgaa
    12061 agatcctcaa aaattagcag aagcattttt tagtgaaatg acggaagagc agctaactgc
    12121 aaaccaagaa aaatggctcg gagaaacgtt gcaagaaatc atggaaatgg aatgatcaag
    12181 aatgaaaccg aaaaaattac aacttaaaaa ttttggtcct tttatcaatg agaccgtaga
    12241 tttttcacgt ttgacagaag caccgctttt tttgatcagc gggaagacag gagctggcaa
    12301 aacgacgatt tttgatggga tcaccttcgc actttttggt gaaacttccg gaagacttcg
    12361 ctctggaaaa gagatgcgtt ctttgtttgc tacaccagac gaagaaacaa gcgtgacttt
    12421 ttcttttgaa catcagcaga tgagctatga aatcgaacga aaaccagaac aagttttagc
    12481 aaaacgaaaa ggaaacggaa caaaaaaaca ggcagccaaa gtaacgttga ccatttttga
    12541 tgggaaagga aaagaaataa aacaaatcac aaaacgtaca gaagcagatc agttgatcag
    12601 agaactgatg aatctcgatg cgaaacagtt ttctcaaatc gttttactac cccaaggaga
    12661 attccgtaat tttttggttt catcaagcag tgataaagag actgttttac gtcatttatt
    12721 cggtactcaa tttttccaac agttcaatga gcgattaaaa gaaaaagcaa aacaacagca
    12781 acaaaaattg gatcatttag agcaagagct gcatcttctg caaaaaaggt ttgttttagt
    12841 caaagaagag gaaccagcag cagggtttga agccgtttta gcccaatgga caaaccatca
    12901 ggcaatactc gaaaaacaaa tcacggaaga aaaagaagct ctaacggaac ttcaggaaaa
    12961 acaaaagcaa ctagaaaatt cctactatcg ttttgaaagc ttgaaaaaaa gttatgaaga
    13021 aaaaacgata ttaataaaca agcaaaagga attagctgct caaaaagacg aaatagagga
    13081 aaagaaacga tggattgggt attatgaatt tacgcaacga ttagtcgaac caatccagcg
    13141 cctacaagaa ataaaagttg aaaaaataga actgtcacaa aaaagtagtg agcaaacaaa
    13201 gaaactagcg atgactacgc agaattatga agagtggcaa aaaaatgaag caaaccgcgt
    13261 acgccgacaa acaaaaatca aagaagctgc tcaaaaactg caaaacaaac agatgatggt
    13321 gccaattgtc gaatccttac gtagtaaaaa acaagacgca gaacaattaa caatttcatt
    13381 ggataaagaa gaattgaatg ccgagaagct gtatcaaaat ctcgctatct atcaggaaag
    13441 aactcgaatt cttcaagaaa atttactgga tcaggaaaaa ctgcaagaag atcgtcttgc
    13501 ttttggacag ttaaaatatc tagatgaaaa gcagcaagaa atagaaaaac aaaaagcaaa
    13561 agacactggg tcgctcgcac agataagaga agaactcaaa aaagcaactg aagaaataac
    13621 aaaaaatagt cagctattga aagaacagac agaacaagca gaacagctga aaagccaatg
    13681 ggcaaagcag caaattgctc gtcttcagct ttttttgcgt ccaggggagc catgtcttgt
    13741 ttgtggatca acggaacacc ccatacaaca attaaaacac gaacagccaa ataaggaaga
    13801 tatcttaaaa gtagaacaac aactggcaga agctgagtct gctgtagaaa gaactcagca
    13861 aatcattgcc aaaagtgaag cacatgaaaa taatcttcaa caacaattat cagatttaga
    13921 acaaaaaatc atggcaacga atatacaatt ggctgaacaa gaagaacact tgcaacaggc
    13981 tttgaaaaag gcatttcctg agataacaga acaaaaaaca aaaacaactc ttgtagtaat
    14041 cgaacaatct ttagatagac gaaaaagcaa gatggccaat gcaagaatag agctggaaga
    14101 aataagacaa caagcagaaa agctcgaaca acaattagct gtctacaaac agcagcagac
    14161 agaaaaaaat aatcagctcc agcaactgca aggagaaatc aaaagtttag aacaacaatt
    14221 gcaagatacg gatagtaact gtttagaaga ggagatacga cgacttgaaa aacagttaga
    14281 agaagactcg aatcaactag ctgttgagca gaaagcagga gtgcaattgt tgcaagatct
    14341 tcatacgctt cggacacagc ttgatcttca acatgaacaa atagaaaaaa tagatgaaaa
    14401 aatagaaaag gaacaagaaa gaatagctaa aaagttagaa agccagtcgt atttttcaac
    14461 aattgaagaa atctgtgcat ttgcacaaca gcaagcacaa tataaagaaa accaacgtac
    14521 agttacttct tatcatgaag agcaattagt gacagctgac cggctgcgtc aattagaaaa
    14581 tatccagcta actgagacat tcccagatac agaagagatc agccaacagt tagaagaaat
    14641 aagacgtatg atcgaaataa agcaagaaaa gatatacggt ttacaagaac aaaaaattag
    14701 taatcaaaaa atctacgatg aatgtctttc gatttatcac acaagccaga cgaaattaga
    14761 tgagcttacg caattacagc agctttcgca aacattcaac ggagaaaatc ctaagaagac
    14821 tagtttggaa cggtatgtgc ttcaggttta tcttcaagaa gtgttacaag tagcaaatga
    14881 tcatctccaa cgtttaacga aaaatcgtta tcaatttgag ctagcggata ccattggcag
    14941 ttatcgaggg aaaaccggat tagaaatcaa tatctatgat gatcaggcag gtatgtctcg
    15001 aagtgcgcat accctgtctg gcggtgagag ttttattgca gcactttctt tagcattagc
    15061 tcttgccgat gtgatccaga cacaagctgg tggtgtagca atcgatgcat tgtttattga
    15121 tgaaggattt gggtcattag atgaagaagc tttggaaatg gcaatggaag ctttagaaac
    15181 gatcgaaagt gaaggaagaa tgatcggcat cattagtcat gtaagagaat tgaaagaacg
    15241 agtattacaa caaataagaa ttgatacaga aggtagcgga caaagcaaaa tacgctatca
    15301 acttggataa aaagggagat tggtaactat gaaatggagt attccagaaa aagtcattgc
    15361 acgtggacgt caatacttgc aagaaggccg agtgttatcg gttgtccctg accaagaaga
    15421 aaacgtgtgg catgcagaag tcttgggcag tgaattgtat atggttgatt tggatggtac
    15481 agcaaaagag atagattact gccaatgtcc ctattgggag gaacataaat attgtaagca
    15541 tacagttgct gtggaacttt atttacggtc gaaaaatcta tctcgtatca tgaaaaaaga
    15601 ccagccaatg ctagaaccgg taaaagcaac aagtgaggga gaaatcattg caaaaggttt
    15661 ttctcgtcta aaaacacaaa acaatcctca agcggtgcaa cctttgatta ttgaatacca
    15721 agtagaaacg attgagacta atcagtatca cccagagctt tctattttgg cactttactt
    15781 gcgtatcggt tcattggcat caccgaaaaa aacatatatc gtaaaaaaca tctatgattt
    15841 tttacaagct tacacagaaa aagaaactta cactgttaat aaacagtacc agtttcgtct
    15901 agataaacgg gcgtttcaaa aagaagatca aaaaatactc acgcatcttg caggaagtgc
    15961 tcagacgcat caattattag gtacgaacgg cttacaagtc aaaggaaaac tagataaaag
    16021 atatctgctt ctcccagtag aacaagcccg cttcctgtta gagcagatga atcagactac
    16081 acgtttgcat ctgcagataa atgaacaaaa cgatcaagga atacaatttt ccgatcaaaa
    16141 caaaccttta tcattcaaag tggaaaaatt tggagaagat tatttgctgc aagcattgaa
    16201 tgattttgat tttttcttta tgcattatca gtggggagtt atccaaggga cattttattc
    16261 tctgacgaaa tatcaacaaa cggtttatca aacttggaaa caattgattc gccgattgga
    16321 gaaaccagaa gtagtgttca agaaaaaaga attaccgtct ttgtttaaag agattatccc
    16381 tctgctgtca gagatcggcg acgtagctat tgcccctgca gtagaagagg aagtagcaga
    16441 tatccccgtc gaatttattt tctttttccg aaaagcaaaa ggaaaaatcc aagctcgagt
    16501 cgatttcgtc tatggcgaag tcatctattc gactgacccg aagcatgagg tctcgacaga
    16561 acaggctttt caggtgttac gtgacacagt agaagagaaa cgagtacttg atctattcag
    16621 aatgtatcgc taccagaaaa atgaaactgg ttatgaacgt ccattgccaa gcggggaaga
    16681 actatatgcc tttttccgca cagaactatc tgtcttccgt cagtttggtg aagtcagact
    16741 tggaagaaaa ttgcgagagt tgtatttaga tgcgcataaa catcaaccga aaatcgaagt
    16801 aaccgagacc ggttcatggc tcgatattcg attcgatgtg acagggatag aggagcaaga
    16861 aatcgatcat gtcctgcaaa gcttgctgag aaatgatgct ttttatacat tagaaaatgg
    16921 tcaggttctt tcttttgatt cagaagaatt tcaacaaaca agccaagtgt tgcaacaatt
    16981 acgggaatcg atacgtacag aagaaggaac gatccatgta cctaaaaatc aaggattgat
    17041 cattcaaaat caattggaaa aaagcaatgc tacttttagt gaatcgttcc aaacgatggt
    17101 acaagatctc attcatccag aaagatacca ggcacagctt cccaaaggat tgaatgcaac
    17161 gatgagagat tatcaaaagc aaggttttcg atggttgaag atgttaggtc attatcagtt
    17221 tggcggtatt ttagccgatg aaatgggact tgggaaaaca ctacagacaa ttgccttttt
    17281 actttcggaa aaagaagaac ggaaaagctt ctctgctttg atcgtagcgc ctgcgagctt
    17341 aatttacaac tggcaggcag aggtgagaaa attcgcccct tctttaagta tccaagtaat
    17401 caatgggaat aaaaaagaac gtgaagaatt actggctaaa gacacagata tccgagtgac
    17461 ttcttatgcc agcttaagac aggatctagc taattatcag tcgcaaaaaa ttgattattt
    17521 gattcttgat gaggcacaaa tggtcaaaaa tagcagtacg aaaactgcac aagcattgag
    17581 agaattagct gtacctcagc ggtttgcact gagtgggaca ccaatcgaga ataatttaga
    17641 agaattgtgg tcattgtttg caacgattat gccaggattt ttcccgacta aaacaaaatt
    17701 cagggaactt gccgcagaag aaattgcgca aatgattcga ccgtttattt tacggcgaga
    17761 taaacaaact gtgttgaaag acttgccgga aaaaacagag atgaatctat attctgcact
    17821 tacagaagaa caaaaaactg tttatttagc ttacttgcgt caaatgcgtg aagaaatcac
    17881 atcaatggat tctgaagcat tcaagaaaaa ccggatcggg atacttgctg gtctgacaag
    17941 actgcgacaa atttgctgcg atcctcgtct gtttattgaa gattaccaag gcggttccgg
    18001 caagctcgaa caagtaaaag atctactggt agctgccaaa gagaacaaac gaagagtttt
    18061 gctgttttct caatttacag ggatgctgac tatcttgcaa gaagagttag ccgagctagg
    18121 gatctcgaca ttttatttaa gaggcagtac gaaaccacaa gatcggttaa gtatggtaga
    18181 cgcatttaat gctggcgaaa aagatgtttt tctgatttcc ttaaaggctg ggggaactgg
    18241 attgaatctc acgggagcag atacggtcat cttgtatgac ctttggtgga atcctgccat
    18301 tgaagaacaa gctgctggac gtgcacatcg gatcggacaa aaaaatgtgg tagaagtgtg
    18361 gcgtatgatc tctgaaggaa cgattgagga acgcatggat tcactccagc aagaaaaacg
    18421 cgaactgttc caaaaagtga tccaaggtaa tgaagaacag ttgacgaaac tgactgaaga
    18481 agacatccgc atgattttaa gtgtaggaga aatcgaggag taagagactc gaaaaagcgt
    18541 tcagtaatgg gtaagaaagc ctttctaaag aagcgattgt ttatttgatt taaaaaatag
    18601 caaggaggct gggattccat tttatcccag cttccttgct atcatttttt tatttctatt
    18661 tttaaaggtt atacaaagat tgtcttcatt gtctctaaca gctgatcaaa agaagaaatc
    18721 tcgatatcgc aaattggagt ctgttcaatt ttgcgttctc gatggttgaa ccaaagcgct
    18781 tgccagttag cactttttgc acctagaaca tcattgtcgt aattatcgcc aacgtataag
    18841 gtctcttctg gaagcatatg aaattctttc tcagctagct gaaaaatctc tttttctggt
    18901 ttttggaaac ctgttgcttg agaaatgagc atattgcggg aaggaatcca attgttcaat
    18961 tgaagttgct tcagtttttt tgtctgatgg tcagtcggcc catttgtgat aattcctaat
    19021 gggacattct tttccttcaa gaaatcgagg gtttttttga cctcctcatg caagcagata
    19081 ttatcaagtt cttcctcgta aattttttga aataagaggc catcttcttc cgtaatatga
    19141 gggtagtcta aatcttttag tgattggctg atccgatgtg ctcgcatata ttctagtgtc
    19201 caatctcctg ccattacttt tgggaagttt tcatcactat gatggcggaa ccggatatat
    19261 agtggatgca tatctgcatc tgaaacaagc ggaacgactc ttttgaccgc gtttcgaaat
    19321 ggctgctgct gatcatacat cgtatcatca acatcaaaca caactgattt aatcaaaatt
    19381 ttacacgcct ttcggttttc attgattttt tcacaaatcc ttgactattg taacagaaaa
    19441 aattgtctaa aaaagctgat aaataaaggt ttttcttact atatttctat ttccttgaca
    19501 gaataaggtt tttatatttc agtttttttc actaaaaatc gtgaaacacg tcaataagtt
    19561 tgatgaacaa attaatcatt atggtagaag tcgattgatt tattttaatg caatctctgt
    19621 ggaattttcg aaagatttat ttttttaata cagtctcaat ataactattc aaacataaaa
    19681 taacgttatt tgatttattt gtttatagta aattttattt agtaatcatt gctttaaaat
    19741 actacgctta tataacagag aaaataagcg ttataataaa gaacagtatt atttaaaatg
    19801 acagaaaaca ttactattaa agaaataaaa aacataaaaa atcttaagaa atcggtttca
    19861 tttagctttt gagataaaat aatagtacag ttttatacgc tgtttcatat gtttagaaaa
    19921 tttgaattag atttgaaaac tctaattttt aaatgataaa aaaagcgaga atgctggttt
    19981 tactgtatat taaccttaga taactttcat ttttttaaaa aaattatgaa aaaaaggtag
    20041 ctatttaaaa gttgattatg tataatagaa acagatggga gattattatg atcttctgtt
    20101 acaaaaaagt gattcttatc acgaaaagga gtggtgcacg ttgttaacat tatatacttc
    20161 cccaagttgt acttcctgca gaaaggctcg tgcgtggttg caagaacatc agattccatt
    20221 tgttgaacgt aatatttttt cagaaccatt gaacagttct gaattaaaag cgattttaca
    20281 aatgacagaa gacggaacag aagaaatcat ctcgacacgt tccaaagtat tccaaaaact
    20341 gaacatggat ttagatgatc tgccgttaca agaattattg gaacttgtgc aaaacaatcc
    20401 tggattgctt cgccgtccaa tcatgattga tgataaacgt ctgcaagttg gttttaatga
    20461 agatgaaatt cgtcggttcc taccgcgcga tgtacgacaa ttagaattac gtcaagcaca
    20521 attgatggct ggattataga aggataatac gaaacttgga aaagactatt acaaagatag
    20581 tttttccaag tttttttgtt tttacaaaaa gtattcttga ttagatgtat aaaaaggtag
    20641 tagaagatga tgattgcacg aaaaatagag agggaaaatt agctgaaaag tcggctatat
    20701 agtattcgtc tgccaaaaat gttatagtta aataagatag cttgaaaggt tgaaaaatcc
    20761 gctggtttta ttaaattcgc ttaacagcga gatttttttt ctttacattt aagctgattt
    20821 cgctacaatg aatctatatt aaacattgtg gcaaagtgag gtgtagcaaa atggaaatgg
    20881 aacatattaa tgaaaatacg atccgtgtac taattggtca tgaagattta gaggaacggg
    20941 gcgtttcatt tttggattta cttggcaatc acaaagaaat cgaaaatttc ttttatagta
    21001 ttttggaaga agttgatatc gaagaggagt tccgaggaag cgaagctgtc actttccaag
    21061 ttttgccaaa aggagacgga ttagaactgt ttatcagtaa aaatcttccg gatgaagaaa
    21121 tttctcaatc agaagattct actgttgatt cagaagatgt gagtgacttt cttcgtcagc
    21181 agattattgg cagcgatccg gaggaagaat tatctggaag cgccctagaa gaacaggaaa
    21241 gacaagcaac ttttgctttt gaagattttg aacaagtgat ccaacttgca acagatgtcg
    21301 aactagagtc tgcttggact gatttgtatt tattcgaaga gcgctattac ctagtcgttc
    21361 acttctggat ggaaaatctc aaccaagcgg atgtagagaa tcagattgct cgaattttgg
    21421 agtttgctga aaagagcgat cgtacaccag aaacattgaa tgaatatgga caatgtctga
    21481 tggcgcgtaa tgctatcgag cgaacgaaat tttattttgt ataaatcagc attatagtaa
    21541 gagactgtga caaaaattgt gtcgcagtct ttttttcgta taaatgtgtt caaaagcttg
    21601 catctgcggt aacaagccca accaaacgaa aaatattggg aactattttt gtttagctgt
    21661 tcttattact tgatgcagaa tagctttttc acgccctctt ttttcgtgta aataaattct
    21721 tagacgtaaa taagttcaga gggagaaaaa tgctgcttgc aatgaatgaa tataataagt
    21781 gctgttttgc taaacatgca gaaaaaggaa agaaatatta ttgtcgtggc tgtcgaaaaa
    21841 gagtgatttt gaaaaaggga aagaaaaaat gtgctcattt tgcccatcaa aaatcagata
    21901 actgttcggt ctttagtgaa agagaatcaa aagagcactt acagttaaag gaatgcttca
    21961 tggattggct gggacagtca gtagaaccag tatttttgga agcctatttg ccgaggttgc
    22021 ggcaaaggcc ggatattttg ttggcgaatc tagcaataga aatccaatgt tctagactgt
    22081 ctcaccagag atttattgaa agaacccaaa attatttgaa taacagttac caagtatggt
    22141 ggatacttgg taattctttt cttggacaat cgcaattctc attgatagaa aaaagttgct
    22201 gctactataa tagaaaaaga ggagtgcatt gctggaaagc tgatctcaag aaacaaaagc
    22261 tttatttgta tcatcatatt acagagactg tatcagggca tatttccttt tgcagtagct
    22321 gctggacttt ttctagtagg gaccttaaag aaatattcac tagtaacgaa attaaaatga
    22381 accaaatgaa aaaaatagaa aaagtgagtg aagatgggaa gaaatggctg acacgccaac
    22441 ttattcacaa gcaaaagaat acagtatcca tacaagaaca atgctacttg cgtcataaac
    22501 atcttttgca tctatcatcg tggatctatc aaaaaagtcg gtttttcttt tatttacaag
    22561 aacaggtttt tctttatcga atgttgtacg aagaagcctt ggaaaaccag aaagttcctg
    22621 atttcaactc atggctttgc caaataaaag aacataaaag ggaatggctt tttcctatga
    22681 ttgatgaaga gatggtctat cagcagtttt ttaacgaatg catacacttg tcttctttga
    22741 aaaaatagta agtataaaaa taggctagga atcggttttc tatgtaaaac aagatttagt
    22801 tatgctaaaa ttgatggaag cagacacatg aatgagtaga aaagttagat gctgtgacat
    22861 gtggatgtta gactatgatc tattttgata gatagagcta agggaagaaa aggagtggat
    22921 ttcatggaag taaagcagtt gccaaaacga gaagaattgc ctgaaaattt aacttgggac
    22981 ttgaccaaga tcttttcaag cgaccaagag tttgatgaga aatatttgga attatcagaa
    23041 gagttaaaac aatctgaaaa acacaaagga acacttgatc aaggcgcttc tcaattttta
    23101 aatgcgattg aattcgtatt gagggtttat cgccaaactg aagtcattta tgtatatgcg
    23161 caccttaaaa acgatcaaga cactggaaat acagattacc aagcgcttta tgcaagagca
    23221 agcagtctgt tttcgaaagt tagtgaagcc gtttcctggt ttgaaccaga aatattgcaa
    23281 ttgtcagatg accagatttg gcaatatttc aaagaagaac caaaattgga agtctatcgc
    23341 cattatatcc agcaaatagt agataatcga gcccatgtct tatctgctga gcaggaatct
    23401 cttcttgctg gagcaggtga aatctttgat gcttcaagtg atacatttgc tgttttgaat
    23461 aatgcagatc tagtttttcc aacgattgaa ggagaaaatg gtgaaatagt ccaattatct
    23521 catggcgtgt atggtcagtt gctagaaagc acggatcgaa gggtgcgcga agcagcattt
    23581 aaggggttgt acagtgttta cgaacaattt agaaatacat ttgcttctac tttaggcaca
    23641 catataaaag gacataattt taaagcgaaa gtccgtaatt acagctctgc cagagaagcg
    23701 tctttgagca ataatcatat tcctgaaagt gtatacgata ctttggtaga cgtggtaaac
    23761 aagcatttgc ctttgttaca tcgatacatg gaattacgga aacgtttatt agaagtggaa
    23821 aaactgcaca tgtatgatct ttatacaccg gtcttagggg aagctccaat tacctttacg
    23881 tacgaagaag caaaagaaaa agctttagaa gcactgaaac caatgggtga agaatacatg
    23941 gccatcgtag aaaaagcatt ctctgaacgt tggatcgatg ttgtcgaaaa taaagggaaa
    24001 cgaagcggtg cttattcttc gggaagctat gacacaaatc catatatttt attgaattgg
    24061 catgatacgc tggatcagct atttacgctt gtccacgaaa tgggacatag tgttcatagt
    24121 tatttcactc gttcgaacca gccttatgtg tacggcgact actccatctt tttagcagaa
    24181 attgcttcga caacgaatga aaatatccta acggagtatt tattggaaac agaaaaagac
    24241 cctcgtgtac gggcttatgt actcaaccat tacttggatg ggtttaaagg aacagttttc
    24301 cgccagacac aattcgctga attcgagcat ttcatgcata cggaagatga aaaaggtgtg
    24361 ccattgacta gtgaatacct aagtgatagt tatggtaaat tgaatgcaaa atattatggt
    24421 ccagcagtcg aagaagaccc agaaattaaa ttcgaatggt cacggatacc gcatttttat
    24481 tataattact atgttttcca atactcgact ggcttttctg ctgcttcagc actggcgaag
    24541 aaaatactaa accaggaacc agaggcacta gagaactact tggcttacct aaaagcaggt
    24601 aacagcgatt atcctgtgga agtaatgaaa aaagcaggag ttgatatgac acaggctgca
    24661 tatattgaag atgcaatgtc gatgtttgaa caacgtctaa atgaattaga agaattgatc
    24721 gatcgtttat aaggctttct atttttgatt ttctcgaata gaaacgaaaa aaatacgtcg
    24781 gctctcttgt aagggagtcg acgtattttt ttaaggctct atactttttg atgttggtga
    24841 acttcaccaa catctttttt gtat
//
LOCUS       DACEUF010000041        23811 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.042, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000041 DACEUF010000000
VERSION     DACEUF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.042"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            161..598
                     /locus_tag="IXG20_001550"
     CDS             161..598
                     /locus_tag="IXG20_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609752.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            693..1577
                     /locus_tag="IXG20_001551"
     CDS             693..1577
                     /locus_tag="IXG20_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1609753.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1801..4968
                     /locus_tag="IXG20_001552"
     CDS             1801..4968
                     /locus_tag="IXG20_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609754.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4988..5533
                     /locus_tag="IXG20_001553"
     CDS             4988..5533
                     /locus_tag="IXG20_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ1609755.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5561..6016
                     /locus_tag="IXG20_001554"
     CDS             5561..6016
                     /locus_tag="IXG20_001554"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ1609756.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            6013..6771
                     /locus_tag="IXG20_001555"
     CDS             6013..6771
                     /locus_tag="IXG20_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1609757.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6857..7597)
                     /locus_tag="IXG20_001556"
     CDS             complement(6857..7597)
                     /locus_tag="IXG20_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1609758.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7658..8911
                     /locus_tag="IXG20_001557"
     CDS             7658..8911
                     /locus_tag="IXG20_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1609759.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9233..9460
                     /locus_tag="IXG20_001558"
     CDS             9233..9460
                     /locus_tag="IXG20_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1609760.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9583..9909
                     /locus_tag="IXG20_001559"
     CDS             9583..9909
                     /locus_tag="IXG20_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1609761.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9977..10690
                     /locus_tag="IXG20_001560"
     CDS             9977..10690
                     /locus_tag="IXG20_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1609762.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            11000..11851
                     /locus_tag="IXG20_001561"
     CDS             11000..11851
                     /locus_tag="IXG20_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1609763.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11972..13837
                     /locus_tag="IXG20_001562"
     CDS             11972..13837
                     /locus_tag="IXG20_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1609764.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAEADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13885..15294
                     /locus_tag="IXG20_001563"
     CDS             13885..15294
                     /locus_tag="IXG20_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ1609765.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15402..16103
                     /locus_tag="IXG20_001564"
     CDS             15402..16103
                     /locus_tag="IXG20_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1609766.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16262..16741
                     /locus_tag="IXG20_001565"
                     /pseudo
     CDS             16262..16741
                     /locus_tag="IXG20_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            17589..18164
                     /locus_tag="IXG20_001566"
     CDS             17589..18164
                     /locus_tag="IXG20_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ1609767.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            18164..18604
                     /locus_tag="IXG20_001567"
     CDS             18164..18604
                     /locus_tag="IXG20_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1609768.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            18620..19321
                     /locus_tag="IXG20_001568"
     CDS             18620..19321
                     /locus_tag="IXG20_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1609769.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(19809..20234)
                     /locus_tag="IXG20_001569"
     CDS             complement(19809..20234)
                     /locus_tag="IXG20_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609770.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            20608..21579
                     /locus_tag="IXG20_001570"
     CDS             20608..21579
                     /locus_tag="IXG20_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1609771.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            21611..23008
                     /locus_tag="IXG20_001571"
     CDS             21611..23008
                     /locus_tag="IXG20_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1609772.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            23167..23775
                     /locus_tag="IXG20_001572"
     CDS             23167..23775
                     /locus_tag="IXG20_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609773.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLITAYSEKVDKKVVADRLAEWKKTNEQVKKTTVSSQK
                     NESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         8044 a         3937 c         5050 g         6780 t
ORIGIN      
        1 tttttattag aactaaatca acagtagttt aattactact tgatttagtt ctttttattt
       61 taaaaatttt ttttacttag aatccagcga ccctagatat ttttctgtat aatcttaaat
      121 aaagaaaaca atatcaaaaa atgcacgtag aagtagataa atggagtggt tttgcaaatt
      181 tcttttattt ctagaagtta ttcacgtata cggatttgtt tttctcacaa ataaaaaggc
      241 aggaagtaca atggatcatt tgtatataaa atatattcat caattttccg ttttggtgtt
      301 aggcacacta attatagcgg ggattgtcgc atttattatt caaggccgtg acatattaac
      361 agtacatcaa gagtttttca gttatgcatt tggtattcct ctgatcgcta taaggtctga
      421 taacaaaaag tggaagaaac gctattacat tctcttgcta tttttgtgtt tttatctgcc
      481 acctaagatt tttactttat taggtataca atgcctatca ttttctttta tttcctatat
      541 cattgtttta attttcctca gtttcatagt atggatgttt tatcttgtca aaaaataaat
      601 gaatgtttca tgaacagtac ttcatcacag cctaattttt ttctttatgc tatcgttata
      661 ttaaagaaat caatcagaag ggaaagaaag tgatgaaaaa agttttatta gtggtaaacc
      721 ccagttcagg cggtgaacaa gcaaaggaat ttgagcaatt agcgatagca aaattggaat
      781 cggtgtttga tgaagtggtc gtcttgcata caaaaaaagc aggggatgca aaaaacttta
      841 ctcgcgaagc ggctacggaa gggtatcata gtgtgtttgt catgggcgga gatggaacag
      901 tcaacgaagg aatcagtgga atcgctgaac aagaacatcg gccaaatttc ggatttttcc
      961 cattgggtac ggtgaatgac ttggctcgag cattagggat accgctagaa cctgaggaag
     1021 cgatcaatca tttttcgatt gaatcagtca agccattgga tattggaaaa ataaatgacg
     1081 actattttat gaatgtcgtc gcaattggct ctatccctga atcaatcaat gatgtagatg
     1141 cggagaagaa gacgaaattt ggaaaactag cttatttcat gtcaggaatc aaacagttag
     1201 cttccacaca atcctactcg tttcacgtag aagttgatgg aaagaaggaa gaaattgaga
     1261 gcagtacctt gctgatagga ctaactaact cagtcggcgg ttttgaaaca ttacttccaa
     1321 atgcaaaagt ggatgatggt aagctccatt tggtttattt aaaagattct tctttgatcg
     1381 atacgcttaa aacattgcca gacctattaa aaggagtaga tgaatctacg aaaaatctag
     1441 cctaccttac ttgtgagcaa ttagttgtgt cattagctga tgaggaagaa ttgacgacga
     1501 atgttgatgg agatgaaggg gataaattac ctgtgacaat caaagtattg ccctcacatt
     1561 tacaggttta ttgttaaaat ggaggttgtg accgaggagt tggcgggaga acaccgcttg
     1621 ttcgggataa gggactgtga caagactttt gtcacagcct tttttttgta caaaaaagaa
     1681 atttacacct aatagaaaaa tttttccata aaaatacata ttttaaatat gaaagtacaa
     1741 ttggaaatag attaaaacga taaaataaaa ttgtcaaaac gtcagagagg atggtggaga
     1801 atgaatcaat gtgtaaaaaa gctgattgcg tcgattttgt ttgtcttccc tctcttgatg
     1861 gtgacatccg tttttgccga tgtagagaaa acaaacgaat caacgcaatc agagaatcaa
     1921 agttttgaca agactacttt ttcagaagaa tcgattactt ctgaaactac agaatctcaa
     1981 agtgaggctt ctactaataa aaggcaagaa gaaaataaga caagtgagac ctcagaagta
     2041 gctgaacaaa cgtctgatag cagtgaagcg gcactggcaa gagctgtttc agcggatggg
     2101 tacagcgaag ctgccacaac agaagaactg gcgcagcttc tcgcagacga atcagtaact
     2161 aagatccgac tgattcagcc tttgacttta gacagggaat tagagatcaa acgagatatc
     2221 gttattgatt ttggtggatt cgctcacaat ttcggcacac accatattta tatcaatgag
     2281 atacctcagt cgatcgagtt ccagaatttt aaaggaacag ctgcacatcc aggtggattg
     2341 acaccaaata tcgatggaaa tgccattatc attgcttata tgaccgattt ttggggaagc
     2401 agatatcatt ttacgggaga aataaaattt accgggacac ttgatttgta tgatgggtcc
     2461 aaattaggac tgatttacgc tcctcgtgca actgtcacat tagatggtgt ttccggtgtg
     2521 ttggatgtcc aaccagtgaa ggaaggactg aatgccgctc cgggtaaagc ctatttttgt
     2581 agaagctatc aattgaatgt gatcaatggt agtcaacttt atggaccgta tctagggaaa
     2641 ttctacggtt ttctagattc aggagatgag actggtagtg gaaaaacagc gccaggaatc
     2701 catatcttaa gcggttcaaa ggtctctctt gattatgatc gggcagatgg cgttagcgat
     2761 gaaggagagg cagtcgatac gctacctggt aatgtcatgt ttgaagtcag tggttcggga
     2821 agcgaatttt cggtaaatac aaagatcaat attaccaatg acaacaatcg cggaatcatc
     2881 caaatgagag gttcaggatc gaaagtcact gtatcgaatg gtgggaaaat caccatcaat
     2941 acagcaacta cgggcggatt tcgcttgcaa ggagattcta gtcaaatcca tgtatcaagt
     3001 ggtggacaaa tctcggtcaa aatggctgga gatgacgatc aacccgggaa caatgggatg
     3061 cgttttgtag gaaaaggcct ttctttgatc gtagaaggag ccggatcagt tatcgacatc
     3121 gataagcaga gtgggcgttc cagcgcagtt cgttttgaga atggtacgca aaagctgaca
     3181 gtaaccaatg gaggctcatt gagggtaaga aatacaggtg atggcaaatc ttacgtgaac
     3241 agactcaatc aaggaaatca agcgatccaa ttttatgacg cttctggttt tctagaaagt
     3301 ccaggttcag ctgattttac agtcactgga gaaaaatcaa atatcgatat acgcgcagat
     3361 tccggagcaa ctgtagatac aactggagat attgatttaa attttacggc aggtgaaaaa
     3421 acttatttag tcatggtcgg gaaaacaggg gaagataaga ccggcatcat cagtggggat
     3481 gtgttgaatg tggacttaga atctcctacc tattttgatt tccaaaacaa acggattgga
     3541 agcgagacca atggcatagg cggctgggta ttccaaggaa atggagattc ttctttaaag
     3601 atcgcccatt cagaaatcgc tttatggcga aaaacaggcg caacattcga taactttgaa
     3661 aaagacccgg attatttttc tgatatgaca gacttatcga tcaatgggac cgacttaggc
     3721 aatttcgtat caagccctag tgcaggttta acaaatgaat ttgctcagac aggtgccgga
     3781 atgaaaaatt ataatcgaat cagtgccaat aatcagctcc cgatgattga atccttaaga
     3841 gtgccaacga atgcagataa aaaaatctat gggcatgtcg ttgttcccga aggtgtcgag
     3901 gcaaagccaa gagatgcttg gacaggcgaa gtagaagtga ctttacgtat tacttacgca
     3961 gatacgacga aatcatctgt agaaattact gctctcacgc aaggacaaac agatgaaaat
     4021 gaaggggcag gatataaccc atggggagaa ggcgaacaag gcggcttgtt cgaagttcaa
     4081 gtacccaatg atgagagatt aaaaacggag gatcaagtgc aagttgttgc tgctaaaaaa
     4141 gtaagaggtg gccaagaagt gacagaactg gactctgcca aaacgacagt cgatgtgatt
     4201 cctcctgaac ctgctaattt aagtaccaca ttgatcaatg cagcttctcg cacgatcaca
     4261 ggtacaggtg aagcaggagc cttggtttca ttgaaaaaaa atcaagctgt tttgtcagaa
     4321 acaactattg atgatacagg ggaatttgtt ctttctatcc cagaaaatca actttttgca
     4381 ggagatgtat tagaaattgt attaaacgac caagcaggag aagctactgc taagggtgtg
     4441 cagaacaagc caagtaccaa cgatgaaatt gggaatcaca atccaagtga tacgccagtt
     4501 gtttatcatg atgccccgcc ttttgcacct gctacgaaga tcgtagtaga aggtggtctc
     4561 tcatttcttg ccccttccaa actcgatttt ggtcagatca aagcaactgg tttgacacaa
     4621 gaagcatttg gccaaatgga tatacaggaa aaattatgga tttatgatca gcgagaaacg
     4681 aaaaatccgt ggatcttaac gataaaatta tctcagcctt tcacaaatga tgcaggattt
     4741 tctatgcagg atgtcctata tttcaagaaa ggagaggagt atcaattagt gaatgaagaa
     4801 gaactggaaa ttgtcagagg acaaaattca acgaatgtgc aagcagatta ttcagaatat
     4861 ttaaataacg gtcgggcgtt taaacttgtc ttaaccaaag aaaatcagat agctggtaat
     4921 tactcagcag agataacatg gacattagca gatgcgccga cagaataaag atcaaggagg
     4981 atgattgatg aatcgaatat ggaataaaag tcatcgaaac aactgctctt tacttatgat
     5041 ccttctcctt ggattcatcc tttggcagac acctgtccat gcctccgaat tcaattttgc
     5101 ggtagatttt gaattaccgg ctaatcagat tgataagaac aaaacatata taaatgtaag
     5161 gatggagcct gggaaaacac aggaaatccc ctacacggtc tttaaccgaa cagataaatc
     5221 agtaacagtt gagacattga tccactccgc tacgacgaat tcaaacggtg tgatcgaata
     5281 tggagaatca aaaagtaaat ttgatgaaac attaagttat gatatgaaaa acttagcaaa
     5341 aacagtggga gaaataactg ttcctcctca aagtgcggtc actgagacac tttcactgaa
     5401 agcgccagaa aaagagctgg ctggtgtggt cgcaggaggg atcacgtttc aagaaaaaaa
     5461 agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat cagttttctc aagtgaaagc
     5521 aatcttgatt tgaaatagcg aaaaagagtc ttccccagat ttgaaattaa aaaaagctta
     5581 tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat cttcaaaatc caaatgcttc
     5641 ttatatgttc ggcttgacta catcggttga aatctacaaa aaagaaacaa aagagcctta
     5701 cctttcaata aaaaaagaaa aaatagatgt agcacccaat acaaattttg atttacttgt
     5761 cccattagaa ggaaaaaaat tagctccggg acattatcaa gcggttgtga agggtgcttg
     5821 gaaagaaaaa cgctggaatc tccataccga ttttgaaata actgatgatg cagcggacca
     5881 attgaatgaa aaggatattg atttaaaaaa tcagccagaa acatttgact ggcgatggct
     5941 gatacttgtt ggagtcattc ttattggctt atttgctgga ttatttattt taaaagaaag
     6001 aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg ttctagctgg aagtctatta
     6061 ttatgtacac aaagtgtttt tgctgagaat aagagctcag ccactacaga aggaacgatt
     6121 tctgtgacca aaccagatgc tgcaaatcct gaagacgttg ttccagttcc tcctgttgat
     6181 ccaaatgatc ctgatactcc ttttacacct gacgaaccaa atcagggaac atccggatta
     6241 ctgacgattg accaagctcc agatcttgat tttggaacaa tcaagttagg tacagcaaaa
     6301 acagtatatg ctgcattaca aagcggaacg gacagtactg gagcacaaac acaagtacag
     6361 aatttcgtac aagtaacgga taaaagcgga aattatgccg gttggaaatt atcagtcaaa
     6421 cgaacagaat ttattggtac aacatctgat acgaataaac taactggttc aaaggtgaaa
     6481 ttcaaaaacg ccgtgattcg aaatagcgca actaatagca gtggaatgcc atctaccgtt
     6541 tctggaactg gcgctgaagg aatcgaaatc ccagcagatc aggcagtaga tctagtaact
     6601 gcaggagcaa atgaagggat tggcacatgg atttattcat taggagagaa tgtggaacaa
     6661 gggagacaat cagtggaatt atttgttcct gtatccaatt atgctgtaga caactacgtt
     6721 tcaacattca catggtcgtt aacagatgct cctacagaag aggaagcgta aaatcatatc
     6781 aacgatttca gggagtggag ccgattcaag gcccactccc tgaaatctgc tatgcttacg
     6841 tctcagctct tattcatcaa ctcgaagcag ttttttggaa gtcatggaaa cgttcctttg
     6901 tcttttcaaa gatctttcga ccaacgagta aggagtcacc gttgaaaaat tctaacacat
     6961 aatgagcaga atcaattttc tgcaattgcg ataagttgat acagtaagat tttaaaagta
     7021 aaaagtaagc atacttttct aactctgtct tcaattgttt taaagaagta ttcactaatt
     7081 tatcttctgt agttgttttg accataactt ggtttttcaa acctttgata ctttcaatat
     7141 ataaaatttc ccgaggatac aggacgagac ttccaccttt ttgatcaata aagattttat
     7201 cgacatttac gatctgctgt tctttccaat tgatctgttc tagcacttga cgaatttgtt
     7261 cagataaatt cctgtctttt ctgatatatc caatcggaag gattttccga ttgatgatct
     7321 ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt actatgtgca ttttttttac
     7381 gtatttgttc ggcgacatca atacctgtga gtgattgctt caagtcgata tcgataaaga
     7441 aaacatcgtg attatcaata gtgcccaact ggatcaactg attgatttga gagatagaag
     7501 tgagctgcac gatattctcg atatgatgat tgaccaattc tctgatgatc cattcgctgt
     7561 gaaactggtt atcttctatg ataaaatact ttttcatatg caggctccta ttaaaaaaat
     7621 atgtgtacat tttgaaacaa aacaatggag gaataagatg gtcgcaacag tgacacttta
     7681 tttgaccttt actttgattt tagatttagg tatctattta ttcttttcga aacaactgct
     7741 agtaaaaaac agctttggtt gtatagcttt gatggtggtg acacttttac ttcctatccc
     7801 taaagtgagc tttccttacc tagttggctt attattgatg tttctttttt attttactac
     7861 gaaaaacctg tttatcagct gtttgatcag taatcttttt tttgctatca ctggactcag
     7921 cttttttctg gtttttgatc tgatggatta tttaggaaag agacatcttg gaacagcaag
     7981 tttttttgca ctacttatcg catttatttt ctatctatta gtaaaaagta tagacaaaca
     8041 gacgagattt ctcaacttgc tttttactta tccaactgaa cactattttg gaggtatttg
     8101 tattttatta agctggggcg tgatttactt ggtattggga agtgttcact tccgctcatc
     8161 taattatctg cttctatctc ttttgtcgat agtcatcagt tttttttctt tgtttttttc
     8221 cattatgttg atcagtttcc aagtaaaaga aagacaacat caggaatcgc ttcaactgta
     8281 tcaaataaat gaagaatatt accatcatct agaggaattc aaacatgatt ataaaagtct
     8341 actgttttca ttaaaaatta ctcttcagga aaataaaaaa gaaacgttgg aatttttaga
     8401 aatattagaa gatcattctg cagctatttt tgaaaaaccg caaatacaac agctaacaaa
     8461 tattgtatcc ccagctgtca ggggtgtctt tttaaaattt ctagaaaaag cagaatcgga
     8521 gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa acaatcccga ttgatctggt
     8581 tgtttttatt cgctgtttat ctatttttat ttctaacgca ttcgatcatc gtgtagccga
     8641 tcagagtccg attacgatca atctgctgga agaaaaagag ggatcacgtt ttagtatagc
     8701 gaacaaggca aatccaacga atttgacgct atcggagatg gtcaaaagag gaaaaacctc
     8761 taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa atgttggatg tttacgaaaa
     8821 tgcagaacta aaaatcgaat tttcaaaaca gaatcactat ttttttgtag aaatgtatct
     8881 ggcatctaga aagagcaaat cagcctactg acttactgtc aaaggataag tttgaggaaa
     8941 gtctacacgg agatagagac aacaacacag catattcaaa agaaacagaa gcgtgatgac
     9001 cgttgtcacc cttctgtttc ttttgctatg tagttaaaca aagtagttga cacttccgct
     9061 acttagtatt agtacgaaag tatacttcat gatactaagt agataagaag tgaaaagcgg
     9121 aacccacgat cgtcaaaatg ctaactgctt tgcttacacc agataaaggg aagagcaata
     9181 tcgcttattt taggcgtgga gaagcaagca aatccctacg atcacaaaaa aaatgctgac
     9241 ccagcagtta agagagttag aacaagatga aagtatccat cgaaaagcct ataaccaagt
     9301 cccgcctaaa gtagagtact cgctcaccga agaaggaaaa tcattaagag agattctagt
     9361 agcgttgtct gtttgggaag aagaacgtat cgaaaaacaa aaagcctcaa gggaagaaga
     9421 cacgctactg catacacatg gtggctacct aaattattga ttttctatga catttatttt
     9481 aaataaaaga aaacaggagt ttttttaaaa gcatcatcta ttttttatgt aaaaaaatgg
     9541 tatgattaat caagtaagaa gtgattggaa gaaggcaagc aaatgaatag tatggaaaaa
     9601 gtcaaccagt ttagagatga acgtaattgg cgacaatttc ataacgaaaa agatttggct
     9661 atctctattt ctttggaagc atctgaattg ttagaacttt ttcaatggaa aacgccggaa
     9721 gaagtaaaag aaacacaatt agaacgaatc aaagaagaac tggcagatgt attgatctat
     9781 tcatacatga tggcagacaa ccttcacctg gatctagatg agatcattga agaaaaattg
     9841 atcaaaaata atttgaaata tcctgtagaa aaaagcgcag gtgtacgaaa aaaatacaca
     9901 gagttgtaga aatgtgtcat tcggctgttg gaaagagcaa aagaaaaaca gtaaaatgag
     9961 tctggaggga gagaaaatga aaaaaaaagg aaaagcaaaa agatggctag tcaatatcct
    10021 cttgttcctc ttgttattag taggactagc tttgattttc aatgaacaga tcaaggatta
    10081 ttttgtcaga gagacaggag ataaatatgc tatagcgaat gtaacaaaag aagatctgaa
    10141 aaagaataac gataaagatg tcagttttga ctttgatgcg gtagaaccaa tgacgacaga
    10201 aggggtcatg aggtcgcaaa tgagaggaac agatctacca gtgattgcta gtatcgcagt
    10261 cccttcagtt tcaatcaatt taccggtttt caaaggcttg gataatacgt cactacttta
    10321 cggagcagga acattatctc cagatcaaga aatgggaaaa ggaaattatg ccttagcaag
    10381 ccatcgtgca acgaaccctg aattgctgtt tacaccgcta gaaaatttag agatgggtgc
    10441 caagatctat ttgacagatt tagaaaatgt gtatacatac aaaacatttt ttaaagaaaa
    10501 agttgctcct acagataccc aattactgaa tgaagtcgag ggaaaagaaa ttgtcacact
    10561 aattacttgt ggcgacatgg acgccgtcac gcgactagtt gtacaaggag aactggaaag
    10621 tgttacatcg ataaaagatg cgacagatga tatgagatct gctttcaatc tagagacaaa
    10681 aacattttag gatactggga cataagtcgt tacagattaa aaacagccaa aggatatgaa
    10741 tcgataagga aaaaccaatt tgctgaaaaa aagagaaacg caaagaggtg catctctgat
    10801 ttggttgttt tctagataga cttgtgttcc agttttttta ttttttatat gaaaaggctt
    10861 gcataaataa aaattcgatg gtatgatgat tttgaataag aaatggattg ttactgtacg
    10921 gcaggcaaaa ccagaattca gctaagaaac agtaggcgaa ttctaggttt ttttattttt
    10981 cttgagaggg cgaagcaaaa tgaaaattga taaaattttg aataacaatg tcgtcatttc
    11041 gaaaaatgga ttcggggaag aagtcgtttg tatgggaaaa ggactagctt ttcaaaaaaa
    11101 gattggggac gagatttcgc cagaagcggt ccaaaaagaa tttgttttaa gagattcttt
    11161 tgctaaaaac caattccagc agctgatggc tgatattccg gcagaggaga tcgaatgggt
    11221 caaacaagta gtcgaactgg ctgaagataa actaaaagtt gaattttcgc ctaatattta
    11281 tctgacattg accgatcatc ttcattatgc gatcacacga gcaaaagaaa atatccaact
    11341 gcctaatcca ttattatttg aaacaaaaaa attttatccg aaagaatatc aagcagcgaa
    11401 agaggctctt ttgttcgtga aacaaaagac gggtgtggaa ctttctgagg atgaagccgg
    11461 ttttattgcc tttcattttg tgaatagcca acaaggaaac gaagacatgc aagtgacgat
    11521 gaccgctacc acaatggtaa gagatatact gagtatcatc agtaaattct tcggcatcgt
    11581 gttcgatgaa gaatctttga attatcagcg tatcattact catctgcagt tttttgccca
    11641 gcgttatctc aaaggggaag gatctgacga gcaagatgaa tttctctacg cattagttca
    11701 aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc aatgagtatc ttcgacaaac
    11761 gcaaaaacag gaaatgggga tcgcagagca gatttattta acgattcata ttcaaagagt
    11821 agtaagtgag aaaaaaagca tccaatcata aggattgtta ctgtatggca ggcaaaacct
    11881 ggattgaaaa aataggttca cgaatgtttg tgactgtttt tttcaatctg ggtttttatt
    11941 ttttaaataa aaaagaaaga aggaaaaaac aatggataat caagcagttg gtcgtcgtgt
    12001 atgggaagcg gtaggaggac aaaagaatgt caaaagttta gtacattgtg ctacgcggtt
    12061 gcgattcaga ttaaaagatg aatctttagc agatacgcaa aagttaaaag aagacccgga
    12121 cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta gtgattggca gtaatgtagc
    12181 agatgtttat caatcgatcg tggatgagga aggtcttgca gtttcggagg atggaaaaga
    12241 agagtccaaa aatattttga atcgtctgat cgatattatc tctagtatat ttacaccttt
    12301 tctaggggcg atggcagccg ccggggtatt gaaaggattc ttatcattgg caacggtgat
    12361 gggctggctg acagctgata gcggtactta tcaaatattg tttgctgaag cagatggtgt
    12421 ctttaccttc ttacctgtca tgcttgcttt tacagcagct aaaaagttca aagccaacca
    12481 gtttttagca gtagcaattg ctatggcact tgtgtatcca gctatcacag cagttgctgg
    12541 tgcgggagaa gctatcagtt tctttgggat cccagtgatt ctttcaccat ctggttatac
    12601 ttcttctgtc ataccaatca ttctagccgt atgggttcaa agtaaattag agccattcgt
    12661 aaaaaaagtg attccgcaat ttctgcaaat gattttagtt ccgcttgtcg ttttagtagt
    12721 gatggttcca ttaacatttc ttgctttagg accaatcggg acggttgccg gtaatgcttt
    12781 aggcggtctc ttcaactcga tttatggatt tagtccaatc gtagcaggtc taatcatggg
    12841 aagtttatgg caagtattcg tgatgttcgg catgcatcgg ggattcgtac cgatcatgtt
    12901 tttgaatatc gaacaatatg ggtttgatgt attgatgcca atgcttcttc cagccatctt
    12961 ggcacaaggt ggggctgctt tagctgtagc attgcgaaca aaagatacga aacttcgagc
    13021 attagggatt tcttctacag tgacttcgtt atttggaatc actgaaccaa ctgtgtatgg
    13081 tgtgacatta cctttgaaga aaccattcat tgccgcttgt atttctggag gtattggtgg
    13141 agctattatc ggattttccg gagtaaaagc tttctccagc agtttggtca gcttattgac
    13201 gattccgact ttcattaata cggtggatgg tgtagagtca aatgtgacag tagcagtaat
    13261 agctacagga attgcttttg ttcttgcatt cgtaggtaca ttgattttag gatttgacga
    13321 acaaacacaa gacaatcaat tagaaaacaa acatgcgaat gcaggagaac cgattacttc
    13381 agcacggcat accttaaaaa gtcctttaac gggtaaagta ctgccattga gtgaagtacc
    13441 agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc gctattgatc cagaagtagg
    13501 agaactagtt gcacctgcag acggagagat cacgactatt tttccaactg gacatgctgt
    13561 agggatcaca acgactgacg gagcggagat tttgattcat atcgggatgg atacagtaga
    13621 attaaatggg aatggctttg agatcttagt caaacaaggg gatctcgtca aagcaggaga
    13681 tttattgatt cgttttgaca tcgaagcaat tagagcagct ggctatagcg taatcactcc
    13741 agttgtcatt acgaatactg atgcatttgc cgatatactt gaattagacc aaaaagagat
    13801 tattgcaaac gaagatgttc tggcaattgt aaaataagga ggaaatcagg atgcaatcac
    13861 gtttttcaga aaaattttta tggggtggag caactgctgc caaatcaatt agaaggggct
    13921 tatgatcgag acggaaaagg actatccgtt gcagatgcta tgcctggagg taagcagcgc
    13981 tttgcgatta tcggcagtga agaatttgac tggacgattg atcaagaaaa atatatttac
    14041 ccgaatcatc gaggaatcga tcattacgat cgtttcaaag aagatcttgc cttgtttgcg
    14101 gaaatgggat tcaaatgtta tcgtttttcg attgcctgga cacgaatttt tccaaatgga
    14161 gacgaaggta ctcctaacga agcgggatta gaattttatg atcagctgat tgatgagtgc
    14221 ttaaaatatg atattgaacc tgtgatcacg atttctcatt acgaaatgcc tcttcatttg
    14281 gcaaaagaat acggtggctg gaaaaatcga aaactgatcg acttctatga acgatttgct
    14341 cagacagtat tagagagata cagcagtaaa gtcaaatatt ggatgacttt caatgaaatc
    14401 aattcggcct ttcactttcc agctttaagt caaggattag taaaaagcaa tggagcagga
    14461 gagtaccaaa atattttcca agcatggcat aatcaattcg ttgcaagtag caaagccgta
    14521 aaaatcggcc acgaattgcg ttcggatatc caaatcggct gcatgattat ctatgcaaca
    14581 acttacagca tcgatgccaa tccagtcaat caggcagcaa caatgatcca aaaccaagaa
    14641 ttcaattttt tctgcacgga tgtccaagta cgtggagaat accctgcata cacagcgcgt
    14701 acccataaaa aatatggagt cgatccggaa aaattagagc aaacggaaaa agacttccag
    14761 ttgctaaaag aatatccagt ggactacatc ggatttagtt attatatgtc tacggcaatc
    14821 aatgagactg atccacaagc agcaacttca gaagggaact tgctgggagg agtaaaaaat
    14881 ccgtttcttg aggcaagtga atggggctgg cagatcgatc cagaagggct gagaatcgca
    14941 ttgaatgaat tatacaatcg ttaccagaaa ccattattca ttgttgaaaa cggtcttgga
    15001 gccattgatc atatggaaga ggatggcacg attattgatg attatcggat cgattattta
    15061 cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg gtgtagattt gatgggctat
    15121 acgccttggg gatgtatcga tcttgtcagt gcatcaacag gagaaatgag taaacgttac
    15181 ggctttatct atgtggatct agacgatgaa ggaaatggga cactgaatcg atcaaagaaa
    15241 aaatcttttg attggtataa agaagtaatt gcctcaaatg gcgtaaactt gtaattaaag
    15301 cagctgaatg aaaaagaaca cacttctcac atgtgatttg tgttcttttt ttatttttta
    15361 tgtgctattc ttttagtaat agtaaagcaa agggtgacgt tatgaaaagg acacgtgtac
    15421 tatacttgga agtcgcagat aaaattaaag aagatatttt ctcaggaaaa tatccagtcg
    15481 gttcgatgtt gccgacagaa agtgaattag aagaactgtt tcaagtcagc aaaatcactg
    15541 tccgaaaggc aatcgaactg ctggcatcag atgaatatgt cgcaaagaaa agtggtcgtg
    15601 ggacgacggt tctaagcaat cgtccttaca atcgattatc caaagcggca agctttacgc
    15661 aaatcttgga gaactcccga tatgaagtcc ggaaagaaac gctggatttg caattggtcc
    15721 atataaagaa ggaagatcct ttgttttcct ttttcggtga gcaagctgtt tgttttaagc
    15781 gcttatatta tttgaacaat cagccttata tttattttgt tcattatctt ccacaagaaa
    15841 tgatgcaatt cagcagagaa gaatttggca aagaatcttt gtatcgtttg ctgacgaaaa
    15901 aaggatatac tattgactca ttttctgatg atttctcggc agtattttta acagaagaag
    15961 aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa acggatacgg aaatcgtcta
    16021 cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta ccatacggag ctgcatcctt
    16081 accatattga ttatgaaaca taaataaaaa aagaggttgt gaaaaagctg ccctgcatca
    16141 agtaataaga acagccaaac aaaaatagct tctcatattt ttcgtttggt taggcttatt
    16201 accgcagata cacggctgtt tctggattac catttattct ggataagggg gtgtggcacg
    16261 attttcagaa ggaatctatg aaccaatcac tgtattgatt ttagcttcga tggtaacccc
    16321 attcggcttg acgatcgctt atttcttagg gaaaatcatt cgtaaaaata tactcaatcg
    16381 tcaagaaatc gatacattga agaccgcttt tcctatgggg atctgtcaga tcacagaggg
    16441 atgtttccct atcgtattga acgacttggc acgcaatgtc atagcaacgg gagtcggtgg
    16501 gacagtaggt ggcggattaa gcatgttttg gggcgcagat agccgtattc cggcatcggg
    16561 aatgtttgca gtcgctacga tgaccagacc atgggcattc attggtgcat tgttagctgg
    16621 ttcttttgtc acaggaataa ccattttatt attgaaaaaa ccagtagatc cgaatgcaga
    16681 aatcatccaa gaagaaaaag aggaagaaga catatcttgg gatgatttga caattagtta
    16741 ggagaggcag aatggattac aagaaaaaag tacaatagat gtaccttgag acaggaatgg
    16801 tatgaaagaa tcagagttag aaagaatcga atatacagat tttggtttag gagcgatcga
    16861 aactgtccag agcatcggca ccctccttga aatgacgatg aaggaaaaat gagaaccttt
    16921 cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa ggaaacaaaa aaactttcct
    16981 gtactattcc aaaaaggaaa gaaagttatt atacagtaaa aaaagaaatt attcttcatg
    17041 ctagagatca gtacacgttc gagcccaata tcaaacacta gttccaggca ggcgcaacag
    17101 gtgcggttat cactgaattt tcttccagca gtgacgattc aagtgatatt tttacaaatc
    17161 cagctattta tcgttaacaa aaagattttt aggagaagct gatgaacaaa aatgatcaaa
    17221 ttttgatcgt ttagttcgtc ggctttttta tttagagaaa catcatttat cagtgaaaca
    17281 taacacgaga acaaaatgat tttttcgtta ataaaagaaa caagtatttg ttgtttaaaa
    17341 taatacacgt tattggcata taggacgaaa tcccagtatc ttctataagt aagtcatttc
    17401 atatcgatga aaacacgtta tttatttttg ttttatctat aaaatggctg aaaaatgatt
    17461 tttttctaaa tactatatat agtggcgtct atcctatata ccactaaaaa actacaaaat
    17521 ctagtctttt attttaaaaa aacacgtgta gaatgaagat agacttttga gttagggagt
    17581 gtttttttat gaaacttact gttttttgcg gttcccgttt tggtaataaa gaaagttaca
    17641 aatttattgc gcaaacgtta ggaaaataca tggcacaaga agaaatcgaa ctggtttacg
    17701 gtggttcata ctccgggatt atgggagtga ttagtaaaac agtactggaa aataacggaa
    17761 aagtcacagg tatctatccg aatgggcttt ttgaaactga attgcctgga aaagatgtta
    17821 ctacatttat tcctacagaa acgatcgatg aaagaaaggt attattattt gaaaagggag
    17881 atgctgtcct cgttttccct ggaggtctag gtacattgga ggaattctca caacttcttt
    17941 cttggatagc aatcggtctg acgcccgaca agccaatcgg aatattgaat attggcggtt
    18001 attacagcgg cttgcaacaa ttattagaaa cttttgcaaa agaggatttt atggataaaa
    18061 aatggttgga tcaagtaatc ttttccaaca atccattgga actggtcaat gaattacgag
    18121 cagtatctat taaaaatcag ttattattga aggaggcaaa ataatgagtc aaatatattt
    18181 agcaggacca tttttttcag aggagcaaat cgatcgagta agtcgtatcg aaaaagcgtt
    18241 ggaggaaaac aaaacagtca caagtttcta ctctccacgt catcaccaag aaagcaatta
    18301 tgaactattc agcgctggat gggcgcagga agtatacgaa aaagatatgg aagaactgac
    18361 aaatgcagaa tttgttgttg ctatccttga ctttgagcat caaacaatcg atccaggaac
    18421 ggcttacgaa ttaggtgtgg caactatgtt gaaaaaaccg atgatcattg tacaagaaga
    18481 gacggtacct acgaacttga tgattacaca aagtctccat acgtatttaa aatcagacca
    18541 agctgtacgt gaatatgatt ttgaaacatt gcctgttgaa acttacgtag gcgagtactt
    18601 atagaagagg aagggttgta tgtcagtgat tttgatttca ggaacgattg gtgcaggtaa
    18661 gagcagtttg acggatatgc ttgcaaaaga gattgattct aaaccatttt atgaaaatgt
    18721 cgaagataac gaagtattgc cgctctttta ttcaaatcct gagcagtacg cttttttatt
    18781 gcaaatcttc tttttgaaca agcgattttt agcaatgaaa aatgctttag tgaacgatga
    18841 caatgtgcta gaccgatcca tctatgaaga tagcctgttg ttccatctaa acgctgattt
    18901 aggtcgggta acggatatcg aagtacagca gtacgatagc ttattagata cgatgctaaa
    18961 cgagttagat gatgttgctc caaaaaaacg gcctgatttg atggtccata tcaaagtttc
    19021 tttggacacg atgctagaac gtatcaaaaa acgtgggcgt gactatgaac agctagaaag
    19081 cgatgagacg ttatatacct attatgaaac gttgaacacc cgctataatc aatggtatga
    19141 agattttgat gtatgtccta aaatacaagt ggatggggat aaattcgatt ttgtagaaaa
    19201 tccagaagat gcaaaaaagg tgatccgtca gatccaagaa aaattagctg aattggaagg
    19261 aaagcctgct ggaaaatatt ttacggataa ccagcatgca aaaggtcaag aattaggcta
    19321 aaaataaaaa accaaacgac gggaaagaaa aattactacg tgatgtagaa taagcctttc
    19381 tatcgtttgg ttattttata ttcaaatatt tgaaataaat aaatctttac tctttgattt
    19441 gtaaactgaa atgatccact gtttgtttta ctgatgaagc ttgatcaaag gaagaagctg
    19501 agtttgaagt tcataaaaat cagccatagt gagttttgct tggatttgtt tatcttcttc
    19561 tactttttac ataattttga acaggataag attcaaatgt tttaaatgaa tctttgacta
    19621 gtttgtgaca gttgtcgcat atgctcaagc ttccttggtc attaaattct gtatggatat
    19681 ttttgttgtt attacatgta caataatcag gactatcgat catccatttc gtctccttta
    19741 caatcatctt gcttctttac tatagcagaa aagaagggga gaaacaaaag aagatccaac
    19801 agacggcact atagactgta gatgttgcca tcctcatcct tcaaacggta attaccgatt
    19861 tttgtaaata tgactgttgt ttctttttta ttttcttttc ctgaaataat atattcgtca
    19921 tctttggtag tgatcttgat atctgtcagc actgtttttt cttcagatga ttcagaagaa
    19981 tcagttcctt gtgtctgata cgtgcgtgtc cattccattt ttttatctgt aaatgtcaac
    20041 tgtcccgcgg ataaatccat atctttacgt acttttagcg tatatacact gttggtggta
    20101 tctgcttttt ccgcttttaa gacagaagaa tcggttgttt cgtccgagga ttggtcagag
    20161 ccgccaaaca aatcacagcc agtcaatgtc aaaagtcccg cagacaaaat aaatagtacg
    20221 attaatttct tcaaaatact tcccccattt gataggataa agaaattata ccattggaat
    20281 gtaaaacttc aaacggatgg aagagaaaaa cgcacaaaac acccacaaat agagtaaatt
    20341 ttagaataaa attttaaaaa ggcaaaaaat ccgccacttt cagaaagcgt tttaaaaaag
    20401 tgttgcaatc ttttaaaaaa atgctatatt aaaaatgaac cggttcaaat acttgtttaa
    20461 gtataattaa tagtgagagg aagttgctct actgaagact ttagttatat ggatattgat
    20521 gacaagcaac actatgcaaa gaatgcggtc atcataatcg aacaacaaga aagagaaatc
    20581 aaatgtaact ggcaggagtg agaatgtatg gcatcgatcc gtgatgtggc taagatggct
    20641 ggtgtttcag tgggaacagt ttcccgttac ttgaatggtc agcaactgaa ggaaaagaac
    20701 atgaaaaaaa tagcagaagc gatttcagcg ttggactata aagaaaacat cattgctaaa
    20761 gggttgaaga ataatcgaag cttctctatt ggacttttga taaacgggat ttccagccgt
    20821 tttgggtcgg aagttgtctc agggatcgaa agagtagctg aagaaaatgg gtatagctta
    20881 ttattaagcg ggtttgccga tcagccagaa aaaattgaac aaaaaattga atacctgatg
    20941 aagcatgtga tcgatgggct gattgtcttc ttatccgaag aagagtggtc tggatttgaa
    21001 atgctcacaa aaatgagtat tccagttatc gcattgaatt gtccagaagg accagcaggt
    21061 attgatacaa tattagtgaa tgatcgagaa agtgtagcaa aagtgatcgc tcatcatggt
    21121 gaacaaggac ataaaaaaat cggatttatt gctgccactc aaacagacta cgtagcaaga
    21181 gaacgtttag caggagccaa agaagcggtc caaaatgatc catcgatcca attagaggtc
    21241 tttacaggag actattctag aaaaagcggc tattacgggg caaaagcatt attagaaaaa
    21301 ggggcaactg ctatttttgt cagcaattac aatatgtcga ttggtgccat cgaatgtttc
    21361 aatcaagaag gtgtcagaat cgggaaggat attgcgttca gtcactatga ttatttagat
    21421 aaaaacgatc tgagtatcct tccgaaaatc acgatcacgc caccaacaga taaaatcggg
    21481 gaacgagcag cagaacagct gttaaaacga atcaaaaaag aagaggaaga aaatgaaaaa
    21541 gtgattgtga tggaaaatat catacatgga atcaactaaa acgaactaaa aaagaaaaag
    21601 aggtgctttg atgaaaaaat atcaatttcc agcaaatttt tggtggggtt ctgcagcaag
    21661 tggcccacaa acagaaggaa ggatttcagg cgatggaaaa ggagagaaca tttttgatta
    21721 ttggtacaaa aaagaaccgc aaaagttctt tgatcaagta ggacctgaaa aaacctcgca
    21781 agtttatacg aagtatcaag aggatgtaca gttgatgaaa cagactgggc ataattcatt
    21841 tcgaacatct atccaatgga gccgtttgat acctgatgaa tctggaacaa tcaacgaaga
    21901 agccgttcgt ttctatcatc gttattttga tgagttgatt gaaaacggta tcgaaccctt
    21961 tgtgaatctg tatcatttcg atatgccgat gtatctgcaa gaaaaaggcg gatggttgaa
    22021 ccgagaaacg gttgaagcct atgaaaaata tgccaaaagt tgttttgaat tgtttggttc
    22081 gaaagtaaaa aaatggttta ctcataatga accaatcgtt ccagtcgaag caggttattt
    22141 atatcgatgg cattatccag aagagtcaga tatgaaaaaa gcgatccaag ttggctatca
    22201 tgaagcgttg gcttcagcgt tagccatcaa atcttaccat gaaatggagc aaggtggtgt
    22261 tattggtatc atcttgaatc tgacaccaag ctatccaaga gacgaatcaa accctgaaga
    22321 tgttaaagca gcgcaaatag cagatgcgtt cttcaatcgt tcattccttg atccagctgt
    22381 aaaaggcgag tttccaagtg aattgattga gatagtaaaa gaacttgatc tcatgcctgt
    22441 gatccatgca gaggatttga cggttatcaa agaaaataca gtagatcttt taggtatcaa
    22501 ttattaccag ccacgccggg taaaagcaaa agaaacacca atcgatacta aaaacggtcc
    22561 aatgcctgag gattatttcg acaattatga aatgcctggt cgtaaaatga atccttatcg
    22621 cggatgggag atctatgaac aaggaatcta tgatattttg atcaatgtac gagatcatta
    22681 tggaaatatc gactgcttta tttctgaaaa cggtatgggt gtagaaaacg aagcacgatt
    22741 tatcaaagaa gatggcatga tccacgatga ttatcggatc gaatttgtcc aatctcatct
    22801 gaaatatgtc catcaagcca ttcaagaagg tgccaattgt ttaggctatc atatgtggac
    22861 atgtatggat aactggtcgt ggaccaatgc atataagaat cgatatggct ttatttcagt
    22921 agacttagca caagatggaa aaagaacaat caaaaaatcg ggtcattggt tcaaagacgt
    22981 atcagataaa aacggatttg aggcttaaga aagaatagtt gagagcaaat gcgtgaaatt
    23041 ctccattctt accattttgt gaaaaagaag ttcaattatt tacgatcttt ttcaactttt
    23101 ttgtctttct atttcatttt ttgtatataa tagaatagtc aaaattaaat aggagtggag
    23161 gaaagcatga ggaaaaaaag accgtttctt ctcttttggt tattcggttt tttcttactg
    23221 cttattttat ccgcagcatt ttcaggaaaa atgacagcag atcttgctca gaaagaaaca
    23281 gaaaaaatcg tagcaagcaa agaatcagta gataatttca aaaaagaatt ctcaacttat
    23341 tggtgggcgg agtataaaat aactgccaat tacgaaaaag gcgaattgga catttatttg
    23401 ccaaaaggaa aagaagaaaa tcaatttatt aatatcgatg acgtacttag tgcgatttct
    23461 ctcagacaat atcaatttaa agacaagcat ttgaccgttt tgcttttgac tcatgatggc
    23521 gacaaagtag cccgtttgat caccgcttat tctgaaaaag tagacaaaaa agtggtcgcc
    23581 gatcgtttag cggaatggaa aaagacaaat gaacaagtca agaaaacaac tgtctcttct
    23641 caaaaaaacg agtcgacagg aacaagtcct tcagattctg gcagtggaca aacgataacg
    23701 ggaagtgaag aaaatcaagc ctcaattatt acgaatgagt catcacaagg acaatatcaa
    23761 tcctatggac aatgaaaaac agaggttgtg aaatcagcgt tttgacctga g
//
LOCUS       DACEUF010000042        23199 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.043, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000042 DACEUF010000000
VERSION     DACEUF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.043"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            363..731
                     /locus_tag="IXG20_001573"
     CDS             363..731
                     /locus_tag="IXG20_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1609774.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="IXG20_001574"
     CDS             911..1852
                     /locus_tag="IXG20_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ1609775.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="IXG20_001575"
     CDS             1921..2652
                     /locus_tag="IXG20_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ1609776.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="IXG20_001576"
     CDS             2796..4976
                     /locus_tag="IXG20_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ1609777.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="IXG20_001577"
     CDS             4996..5478
                     /locus_tag="IXG20_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ1609778.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="IXG20_001578"
     CDS             5494..6255
                     /locus_tag="IXG20_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1609779.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="IXG20_001579"
     CDS             6386..7720
                     /locus_tag="IXG20_001579"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ1609780.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="IXG20_001580"
     CDS             8027..8512
                     /locus_tag="IXG20_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ1609781.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="IXG20_001581"
     CDS             8505..9188
                     /locus_tag="IXG20_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ1609782.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="IXG20_001582"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="IXG20_001582"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ1609783.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="IXG20_001583"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="IXG20_001583"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ1609784.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="IXG20_001584"
     CDS             12804..13433
                     /locus_tag="IXG20_001584"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ1609785.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="IXG20_001585"
     CDS             13485..13817
                     /locus_tag="IXG20_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1609786.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="IXG20_001586"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="IXG20_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ1609787.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="IXG20_001587"
     CDS             14643..16016
                     /locus_tag="IXG20_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ1609788.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="IXG20_001588"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="IXG20_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ1609789.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="IXG20_001589"
     CDS             17071..17511
                     /locus_tag="IXG20_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1609790.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="IXG20_001590"
     CDS             17655..18356
                     /locus_tag="IXG20_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ1609791.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="IXG20_001591"
     CDS             18353..19474
                     /locus_tag="IXG20_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ1609792.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="IXG20_001592"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="IXG20_001592"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ1609793.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="IXG20_001593"
     CDS             20853..21761
                     /locus_tag="IXG20_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ1609794.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="IXG20_001594"
     CDS             21796..22068
                     /locus_tag="IXG20_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ1609795.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="IXG20_001595"
     CDS             22079..23125
                     /locus_tag="IXG20_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ1609796.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7817 a         4042 c         5085 g         6255 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaacgg ggagcgtcaa taattttgtg taaataact
//
LOCUS       DACEUF010000043        21782 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.044, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000043 DACEUF010000000
VERSION     DACEUF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.044"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(531..2027)
                     /gene="lysS"
                     /locus_tag="IXG20_001596"
     CDS             complement(531..2027)
                     /gene="lysS"
                     /locus_tag="IXG20_001596"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ1609797.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2138..3142)
                     /gene="dusB"
                     /locus_tag="IXG20_001597"
     CDS             complement(2138..3142)
                     /gene="dusB"
                     /locus_tag="IXG20_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ1609798.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3143..4030)
                     /gene="hslO"
                     /locus_tag="IXG20_001598"
     CDS             complement(3143..4030)
                     /gene="hslO"
                     /locus_tag="IXG20_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ1609799.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4247..6358)
                     /gene="hflB"
                     /locus_tag="IXG20_001599"
     CDS             complement(4247..6358)
                     /gene="hflB"
                     /locus_tag="IXG20_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ1609800.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6448..6993)
                     /gene="hpt"
                     /locus_tag="IXG20_001600"
     CDS             complement(6448..6993)
                     /gene="hpt"
                     /locus_tag="IXG20_001600"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ1609801.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6993..8438)
                     /gene="tilS"
                     /locus_tag="IXG20_001601"
     CDS             complement(6993..8438)
                     /gene="tilS"
                     /locus_tag="IXG20_001601"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ1609802.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8558..9028)
                     /locus_tag="IXG20_001602"
     CDS             complement(8558..9028)
                     /locus_tag="IXG20_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ1609803.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9074..9499)
                     /locus_tag="IXG20_001603"
     CDS             complement(9074..9499)
                     /locus_tag="IXG20_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ1609804.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9566..9835)
                     /locus_tag="IXG20_001604"
     CDS             complement(9566..9835)
                     /locus_tag="IXG20_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ1609805.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9846..11441)
                     /locus_tag="IXG20_001605"
     CDS             complement(9846..11441)
                     /locus_tag="IXG20_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1609806.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11456..14977)
                     /gene="mfd"
                     /locus_tag="IXG20_001606"
     CDS             complement(11456..14977)
                     /gene="mfd"
                     /locus_tag="IXG20_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ1609807.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14994..15554)
                     /locus_tag="IXG20_001607"
     CDS             complement(14994..15554)
                     /locus_tag="IXG20_001607"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ1609808.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15907..16881
                     /locus_tag="IXG20_001608"
     CDS             15907..16881
                     /locus_tag="IXG20_001608"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1609809.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17051..18451
                     /locus_tag="IXG20_001609"
     CDS             17051..18451
                     /locus_tag="IXG20_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ1609810.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18529..19251)
                     /locus_tag="IXG20_001610"
     CDS             complement(18529..19251)
                     /locus_tag="IXG20_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1609811.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19330..19836)
                     /locus_tag="IXG20_001611"
     CDS             complement(19330..19836)
                     /locus_tag="IXG20_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1609812.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19959..21758
                     /locus_tag="IXG20_001612"
     CDS             19959..21758
                     /locus_tag="IXG20_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ1609813.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WEEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6044 a         4747 c         3904 g         7087 t
ORIGIN      
        1 agagtgtttc ccgattcatt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagcgattgt caactctttt ttaaaagttt ttttcgattt cgtttttcga aatctttcgg
      121 caactcattt atcataactt atcgtttgtt atttgtcaag aacttttttg aagttttttt
      181 cgatagaatc aagcttcgca agtggttctt ttttaacgac tcagttatct tatcatgtcg
      241 ttagtaacat gtcaataact tttttgttat gtttttgaat aaatattttc ttctgtcttt
      301 tggacaagta ataatatacc aactaaattt agcttggtca acgaattttt tgtatttttt
      361 caagtttttt tcaataaacg attattcata acttttcttt ctttatcgtt cgttttcgtc
      421 ttctttaatg tctagaatag tcggcattag cttttgaact ttcgttatta tttattcctt
      481 tctacacagc aaaagctgcc taaggaattc cttagacagc ttttattcga ttatctcatt
      541 gttgggaata gcaaaacatc ccgaattgat tgtgcatcag ttagcaacat tactaatctg
      601 tcgatcccga tgcctagacc accagttggc ggcataccgt attctaaggc ttccaagaag
      661 tcttcgtcta caccgtgagc ttcgtcattt cctagttcac gttctttttc ttgcgcttcg
      721 aatctttctc tttgatcaat tggatcgttc agttctgtaa aggcattagc aaattcgcgc
      781 cccactatga atagttcgaa tcgatctgtg aatcgaggat cttctggatt tttcttcgct
      841 aaaggcgaaa cttcgactgg atgtccgtat acaaacgtcg gttgtgtcaa tgtttcttca
      901 acaaatgttt caaagaattc attgataaca tgtccgacac ccatagcttc ggttacttct
      961 acattgtgtt tttcggctaa agctaatgct tcttccaatg tcatttcttg ccagaagtct
     1021 acaccagttt gttctttgat tgcatctacc atatggatgc gtttaaagtc actttctaag
     1081 tcgacagctt gtccatcata tgtgattttt gctgttccta gtactttttc agctgcgtta
     1141 cggataatac cttctgtcag attcattaca tcattgaaat cagtatatgc tgtgtatgct
     1201 tctaacatcg taaattctgg attatgtgta gtatcaattc cttcattacg gaaaacgcgg
     1261 ccgatttcat aaactttttc cattccgcca acaattagtc gttttaaatg aagttctagc
     1321 gcaatacgca aatataaatc aatgtccagt gcattatgat gcgtgataaa tgggcgtgcg
     1381 gcagctccac ctgcctcatt gtgcaatact ggtgtttcta cttcaatata gccattgcca
     1441 tctaaatagc gacggatttc gctgataatt tggcttcgtt tcataaatcg gtcaaagctt
     1501 tcgcgattac tgattaaatc caagtagcgt tgtctgtagc gttgttcaat attcgttaag
     1561 ccatggtatt tatctggcag tggacgaagt gcttttgaca aaatcgtgat ttctgaagct
     1621 ttgatagaaa cttcgcctgt gtctgttttc atgatttgtc cagttacacc aaagaaatct
     1681 ccaaggtctg catgtttgaa tacttcatag gcctcatcac ctacttggtc ttttcttaca
     1741 taaatttgaa tttggccttc acgatcttgt agatgcgcaa atccagcttt cccttttcct
     1801 cggcgagtca tcatacgacc agctacacta gcggtcaatc ccatttctgc tagttcttct
     1861 tttgttcttg gatcaaatag ttcatgcaat tcttctgagt tgtgcgtgcg ttcaaatcgt
     1921 tttccaaatg gatcgattcc atcttcacgc aattgttcca ttttttgacg acgtacaagc
     1981 atttgatcat ttaaatcttc tacttgatgt tgttgttcct gtgtcacaaa agttcctcca
     2041 ttctcatgtt aatctctttc ctataatgcc aatgaaacac aaaaaaagca agccgaactt
     2101 ggaatttcgg cttgaaaatt cacgaaaaac ttagaaatca caacgtttgt ttcaatagta
     2161 ctttttccgc tttttctaca aattgatcca gcaggtcatt gatttcttgc tgtttttctg
     2221 tctgattgat tgctactttc actttcgctg ctcgaggaat cccttttaaa taataagccg
     2281 catgtgttct gaattctcta caagctactt tttctccttt gagatctgct aagcgttgta
     2341 aatgaagttt agctgtagca attttttcgg ctggtgttgg ttcaggaatc agttctcctg
     2401 tttcgagata atgctgtgtg cggtgaatca tccatggatt gcccagcgct gcacgaccaa
     2461 tcatcacacc gtctgcaccg acatagtcaa gcatccattt tgcatcttct ggcgttttga
     2521 catccccgtt ccccataaat gggatcgtca aatgtttttt cacttctttc aatacttccc
     2581 agtccgcttt tccttcatac atctgaaccc gtgtacgtcc atgcatagcg atggcacttg
     2641 ctccagctga ttgtgctgct aatgcatttt ctacagcaaa gacgtgctct tcatcccaac
     2701 caatacgcat cttgattgtt actgggatat ttactgctga agaaacagct tggaccattt
     2761 cgtatacttt ctcaggatct aaaagccatt ttgctcctgc ttctgctttg attactttat
     2821 ttactggaca gcccatattg atatctatta tatcggcagc tgtgttttct tcgacaaatt
     2881 gagcagcttc aactagacta tctttatttc cgccaaatat ctgtaaactt aatggatgtt
     2941 ctgtttcttc aatgtggagc atctctaaag tttttttgtt tctgaaatga ataccttggt
     3001 cactgatcat ttcacatacg actaagcccg caccaaattc tttgacagtg actcgaaatg
     3061 ctgaatttgt tattccagcc atcggtgcaa caacgacacg attagggatt tccacattgc
     3121 caattttcca tgtgtgttcc acttaaatcc ctcctaaggc ttctttcttc aatccatcta
     3181 attcttcttc gctatacttg tatttgttat tacagaaaga acaaaccgct tctgcaccgt
     3241 gatcttcgtc tatcattgct tggatttctt ctgcacctaa cgcgataatt gcttctgcaa
     3301 atttttcttt tgaacagtca catttgaatt gaacaggcat tttttccaaa atgttcaatt
     3361 cttctgtacc aagcaatcct ttcaatattt cttctggtgt ctgtccttca tctaagaggg
     3421 tagagattct tggtgtcgtt tttaattgtt cttcgatctt gctgattgtt tcttcatcgg
     3481 cacctggcat gatttggatc atgaatcctc ccgccgtctt gatgctgtcg tcagtattga
     3541 ccaatacact cagtccaaca gctgaaggga tttgttctga gatagcaaga tagtaagtaa
     3601 aatcttcagc gatttctcca gaaacaatcg gcgtttgccc agaaaaaggt tcttttaatc
     3661 ctaagtcttt tgtgacagta aacattcctt gtgttccaac tgctccccgc acatcgattt
     3721 taccttcact atttagagga agactgagat gcggattttt gatgtaacct ttcacatctc
     3781 cctttccatt tgcatctact acaattcctg ttgctggacc atcgccttgg attcgaacag
     3841 ttagtttgtc ttctcctttg agcgtcgcac caagtagcag actaccgacc aatgtccgtc
     3901 ccaaagcagc tgaggaggtg tgccaagtat catgacgtct ttgtgcctcc gctacggtat
     3961 ttgttgcttg aacggcgtat gctcggacga atccgtcgtg agcaagtgcc ttaaccaaat
     4021 aatctttcat ttgttatgta ctcctttttc cgtttttaaa ctcatagtga atcatactag
     4081 caactactgc ttttttcaac tttggaatgc ttcttttaaa aataattcag aatcatctag
     4141 aaaacaggac atcaccctat ttacaactta aataaagaaa tctcccgaaa accggttttt
     4201 gttccggttt tcgggagtca tatcagatca attgacctat atttttttat ttgtattgat
     4261 cgtctgaatg attatcttct gattgttgag aatctgaatc atgttctgct ttattttcct
     4321 cattcagttc atgttttgct tcttcaaaat cacgtttttc ttccgcttgt ttttcagcgt
     4381 ctttttcttc caaagcacgt tttgcttctt caaaagtttt cgcttctttt tcgcttggat
     4441 aatcatttga ctcagtacct tcaggcatca caccgtgttc aaacaatgac ttgattgctt
     4501 tggcatccaa tgtttcgaat tccaaaagtt tttcagcaat cagtttatgc tgtgcacgat
     4561 gagcttcgat gatttctcga gctttatcat gagcttccat caagattttt cgtacttctt
     4621 gatcaatctc gaaagcgact tgttccgagt aagcttttgt ttgaccatag tcgcggccaa
     4681 caaacacttg atggtttcct tcatattgaa caggacctaa acgatcactc attccatatt
     4741 ctgttaccat gcttcgtgct agagctgtcg cttgctcaaa gtcattggaa gcaccagttg
     4801 attggacatt gaagatgatt tcttcagctg tacgtccacc aagtaagcct acgatttgtt
     4861 caaacatatc ttctttcgtc atcaagaact gatcttcttt tggtaaagcg atcatatatc
     4921 cgccggcgcg tccgcgaggg atgatcgtta ctttgtggac gacccgagcg cgactcaata
     4981 ctaatccgac aatcgtatga ccagcttcgt gataagcaac catttcgcgt tcttttttgc
     5041 tgattacgcg atctttttta gctggacctg caatcacgcg atcttccgct tcatcgatat
     5101 cagaagcatc gattttcttt ttatttcttc tagcagctac taacgctgct tcattcaaga
     5161 cattttctaa atctgctccg gcaaaacctg gtgtctgttg agccacaact ttcaagtcaa
     5221 cgtcatctgc catcggttta ttccgtgcat ggactcgcaa gattgcttca cgacctttta
     5281 catctggacg tccaactaaa atctggcggt caaaacggcc tggacgtagt aacgctgggt
     5341 ctagtacatc agaacggttg gttgcagcaa tgacgattac tccttcattt ccgtcgaagc
     5401 catccatttc tacaagcaat tggttcaatg tttgttcgcg ttcatcatgt ccgccgccca
     5461 taccggcacc acgttgacga cctaccgcat caatttcatc aatgaaaatg atagctggtg
     5521 cgtttttctt cgctgtttca aacaaatcac gaacacgact tgctccaaca ccgacaaaca
     5581 tttctacgaa atcagaaccc gagatcgagt aaaacggtac tccagcttct ccggcaacag
     5641 cttttgctaa taaggtttta cctgttcctg gaggtccttc taaaagtaca cctgccggga
     5701 tacgggcacc taattcaaca aatcggcgag gatctttcag aaattcaaca acttcaacta
     5761 gttcttgttt ttcttcttcc gcacccgcta catcagaaaa acgtacgcga ttagcttttt
     5821 tgtctgcttc tttggctttt gatttaccaa agttcatgac acggccgcca ccgccaccgc
     5881 cgccgccttg ttgtcccatc atcatgtaaa agaaaaagat gatgattacg attggcaaga
     5941 agctaagcaa tagggaaacc catataccgc tgcttgattc ttcttttact gttgtagcaa
     6001 ctttgttttc ttttgccagt tctgttactt ggctcaatgt tgtatcattt ggcaaaatga
     6061 ttgttgtgaa atgggtagta gtcgtatctg ttgaacctaa gatagctaac ccaccgctgt
     6121 tagcgacggt ctgctgttct ttgtattcac cagtgatctt atatacacca tttgcaggct
     6181 gcacttcaaa ttctttgatc ttgcctgctt gtagttgttc tgtgaatttt gagtactcga
     6241 tatctgacga tcgctggccg ttattaccaa aaatgaaata aacgaccatt accatcgcta
     6301 agattacgag gacataatac aaggcatttt tcatcatgcc gttgttcttt ttgttcatag
     6361 ttgccctccc taagcgtcaa tttaatttaa cagtttttta tcaatcctga ccattctatt
     6421 gtaccataat ttaattgaga tacagcatta atttgattga taaacactag gtttcaatac
     6481 accgatgtac ggaaggttac gatatgcttc tgcataatcc aagccataac cgaccacaaa
     6541 ttcatttggt acgttgaagc caacataatc tgcttcgata tttactactc ttccttcagg
     6601 tttgtcaagt agtgtaacaa ttttgactga ttttgctttg cggtatttga aaagatccac
     6661 taaataagca agtgttcttc cgctgtcaat gatatcttca acgatcaaca gatcgcgacc
     6721 ttctacattc gtatctaagt ctttgacgat ttttacttcc cctgacgaga ctgttgcatt
     6781 cccgtagctt gaaacatcca tgaaatccaa ttccaaatac gtatcgatag aacgaacgat
     6841 atctgccatg aatggtaccg ctccttttaa gacacctact actaggggat tggtgttttg
     6901 gtaaatttct gttaactctt tgcctagttc tttgcaacga acttgtatct cttcttgtga
     6961 gatcaaaatt ctctcaatat ccttttccaa catcaggttc tccttccaat tgcttctttt
     7021 tgatatttgt aaagaagtat gtagtgtatt ttatcagttt cttcacgaat actcaaatat
     7081 gaaaaggcaa aaggtatcaa gctatatata tttcttttgg catctgtaat gatccaagca
     7141 tgctgccttt gtgaattagg tactttctga tcgataaaat atctagatac tttttttgtg
     7201 agattctctg ttaatttaat gcggtctcct gcttttcttt tttcgacata cagttgcaca
     7261 tcagcaggaa tccaaatgtc atgagaaaat agagaccatg aatctgttgc tttgatgtcc
     7321 cctgcatttt caggtttgac cagaccaatc cattctgttt cattcaaata tattccttgt
     7381 tctggaatca gcaacaagcg ctggtcttct gtctggctag ttgcttgttt ttctttttta
     7441 gagagtacaa caaaatgata ctctcttttc agtatccaat tattgccaat actgagttgc
     7501 cattggcttt ttttctgttg ccactggtct aaaatcgact ccatttgttg ttctttcaaa
     7561 ataattcctg tttttgagaa tgctttgtcg aaaaaggagt aaaaaaaata gtatcgttca
     7621 gcatctgtca tttcttcaat catttctgca gaaaatcgaa attgttcctc ccactgttcg
     7681 acttcctttt cgatcaactg tcccatatat ttttgaatga cttggtctgc ccattggatc
     7741 tgttgcgaaa attgctggaa atgtctcaac gcctgtgtat tttcttgttt caattgagga
     7801 actaccaaat gacgaagccg atttctctgg atatctaaca attggtttgt ctgatcctca
     7861 aagtaaacaa gctgcgattc cgctgcataa gtatataggt cttctttgct aaaactcagc
     7921 aacggacgaa tcagtctgcc cgtagcaaat ggttgtactt ctttgattcc agaaaaagta
     7981 tttaactgtc ccccacgaac gattttcatt aacaccgtct cgatttgatc atctccgtga
     8041 tgagcagtca tcaacgtgtc atattgtcct tcttccatca cgcgagtgaa ttcttgataa
     8101 cgaaagttac gagcttctgt ttctatggcg gatttggctg gatcttccca aatacttaca
     8161 tataaaggga gattcttctc ttcacaatat gagcgaagat aggcagcttc ttctgctgat
     8221 tcttctctta attggtgatt gatgtggacg atacctaaag aaaaaccgat ttggcgttgc
     8281 acttgtaaaa gtaaatggag caaaaccatc gaatcaacac cacctgatac cgctgctaaa
     8341 attctcgatc cttgttgcca gaatccttgt tcattcccgt gccgaataaa tgactgctgt
     8401 aatagcggtg cttttttccc gcctttttca acgttcatcc tttccacacc ctttcttcct
     8461 aaaaaaaaga gagcgggaaa acgtctaaaa cgacaattaa cctgccctct gttgatacta
     8521 tgacccaaat agtcgcacta tcattatttg cggcttttta gctgcgacgt ccgccacggc
     8581 caccgcgctt tccttctgtg tttcgcttca atgaagataa gcggtcatcg ctatctttca
     8641 aaaatgaact catcaatgaa tcgaagtctt ctttttgagc tgaaggttga ctgcgagaaa
     8701 aagatttttt ctgtgctgga cgatttcggt tgtcacgggg atctttttgt tgatattttt
     8761 taggacggct ttcttctcca gattcagcag gttgttcctg cgctttccgg atagatagtc
     8821 cgatttttcc atcatcattt actgaaataa ctttaactgt tacttcatct cctacactta
     8881 aaacatcatg gatgtctttt acatagccat ttgacacttc gctaatatga actagacccg
     8941 tttttccagc acctagatca ataaacgcac caaaatttgt gattcctgat acttttcctt
     9001 gcagttttgc tcctacctcg attgacataa aaaaaatgtt cctcctaatt atttcccttt
     9061 tttatattcc tgcttatttt gcagttgtag aactagttga ttgatctgtt tcatttgtcg
     9121 agctgtctgt atttgattct aatataggat aaactttttc accgtctttt gagtaaaaga
     9181 atttgcttct ggcaagttta gctacatact catcatcttt cagcaatgaa acatcttggt
     9241 gcagctgatc aacgtctgcg ttgacttctt tcatttcagt atttgcttcg actctcaatt
     9301 cgttcaattg atgcatcctt tgcatgtcat ggaaaatctg cacacctatt atcgcgaaaa
     9361 tgaccaaagc accaacaaaa attgcagcca gtctgcgtct tcgaaaaatc agttgacgtt
     9421 gttgttgctg aaattcagca tactttttct ttgcatattc cgtatctagc gcagcgattt
     9481 tattgctttt tttcgtcact tggattcgct ccaatctttt tgattgtctt tttcattata
     9541 ccaatatcgt gaaagcttgt ctaatctatt tctctagtgt ttctgtgcga ttttcagaaa
     9601 taatttcgta cattttttgt gcatcttctt ttttggttga ttcatgtaat tccgtaattt
     9661 tcacttccaa cgttttgttg ccaaactgga ctttcaaaat atcgcctact ttgacatcac
     9721 tggaagattt cgccaattta ccgttgattt gaattcttcc tttatcagct acttcttttg
     9781 ctactgatcg tcttttgatg attcgggaaa tttttaaaaa tttatctaat cgcatatctt
     9841 attcctcact ttttaatttt tttatttaat cgcaggattt tcttaccaaa aggcaacatt
     9901 agccattctc gtactgtaaa cagacgaaga cgaatcactg cataaatgaa cacagaagcc
     9961 ccgatcccaa ctcctatcac gctgacaaaa aaagcagtgg aacgatgagt tacaggacca
    10021 aatactatat tcaatacacc ataatacagc agcagtacta ctgtcataca aagaaggctg
    10081 aacatcaact tccgtccaaa atgccgttct ttccaaaaag catttagctc ttttgtttca
    10141 attcgaacaa aataccaaag cgtcacacct aatcctaaaa gggtagacag gcttgcacct
    10201 gctgttccta ataaaccggt cagaaaagaa gtagtcaatc cttttaccaa tagtccccat
    10261 cctgcgcctt ttaaagaagg gcgaaacgag ttcttgcttt gagcaatact ttgataagct
    10321 tggatgactg cagtgaaagc aatggaaaaa acaaataata caagcgctgc atttcccgca
    10381 taatctttaa acaacgcata attgatataa gggagcagca aggctaatcc caaagacgca
    10441 gctaatgcta aagcggtcgt caatctaaga tagattttgg ctgtctgtaa aaactgacga
    10501 tagatccggt tggtcaaatg acgagtcaac gcaggcagga atgtcgcact taatgcagtg
    10561 gcaatcacta aacctaattg tactaaaggc tgacctcgat catagacccc ttttgctatc
    10621 ttcgcaccgt attctgacag tccgtaaact tccaaagcat tttttacaaa gaaagagtcg
    10681 atcagctgga aaaagataag caatccacta taaatcgaaa ccagtccgcc ttctaccaga
    10741 aaacgccgga tcaagggctt tgacggctgt ttgaacaatg gaaaccgtcg gaagcttaac
    10801 gctcctccat gaatcttttg ttcatagtag tgaaggatcc catatgcgca taaaccgcca
    10861 aaaacagctc cactcattgc tgccgtccct gtttggtaca ccgtccatcc aagctttcta
    10921 aaagcaaacg cactacagac aatcacaaaa acacgaacga actgctcaac tacttgagaa
    10981 acagcagttg gttccatcaa gaatcttcct tggaaattcc ctcgataaaa agatagacca
    11041 ggcatcagca aaaaggtaaa ggaaactact tggatcagcg gtttcaattg tatgtctccc
    11101 atcatccaag caatccagct gctaaatgca aagacaaaca accacaaaaa gacacctgtc
    11161 caaaatacta agggataaag attttgcaaa gcttgctttc gctcgaaagg attctttctt
    11221 tctgcaacgt actttgatat gaactgcgga agaccggata aagctaaagt cattgctatc
    11281 ccataaattg gataaacttg ctgataaaca taaaaacctt cgtcgccgac taagttttgg
    11341 aggggaattc ggtaaagggc acttaatact tttgctatga aagaagcaat cgtcaataca
    11401 aatgctcctt gcatgacgcg ccgcatttct ttatgatcca aatcattgct ccttcctacg
    11461 ctgacagata tttttgctca cgcaacgctt tgacaaactt ttggatttcc tgaagccata
    11521 ctgcttcttc catttggttg gggatcgtca agcgtatcac catctgttct ttttcaacgc
    11581 ctagactggc ttttagtttt gtcgctgtca gtgcttcgaa taactgttcc acactatagc
    11641 gttttgttcc aactttactt agagtaaaga ctatatgcgc ctcctgtttc cttatcgttt
    11701 ctaggagcgc tcgatcgcca tccattttga tctgcccaat tgtcaagaga tgtgctactt
    11761 cttctggata ttctccaaaa cggtcgagca gatcatcttc tagttcatct agcatttctt
    11821 gcgaatcaag ttcacgaatt cgtttataga tctcgatttt ctgccgttga tctgccacgt
    11881 atgtttcagg tagatacgca tcgacaccga ggtcgatctc aacagtggtc ttctccactt
    11941 gactattctt tccttgttta cgagaaacag cttcgcttag catttgagaa tacatatcga
    12001 aaccgactgc atcaataaat ccatgctgct gagcacctaa gagattacct gcaccgcgaa
    12061 tggaaagatc ccgcatcgca atcttgaatc cagatcccag ttctgtaaaa tctttgattg
    12121 cctgaagacg tttttcactg acttcgttaa ggatcttctg ctgctcatac ataaagtaag
    12181 catatgcgac acggctgctt cttcctaccc gccctcgcaa ttggtataac gtggataatc
    12241 ccatataatc agcgttctca acgaacaacg tatttgcgtt cgggatatct actcctgttt
    12301 caatgatggt tgtagtgaca agaacatcat attgaccttc aataaattca aaaagcgtat
    12361 tttctaattg cgcctctgtc atttgcccat gagcataacc gatccgtgct tccggaacga
    12421 gttcttggat ttcttctact ttttgttcga tcgtttctac ccgattatat aaatagaata
    12481 cttgtccacc tcgtcccatt tctcgatgga ttgcttcgcg aatagcccca ggattttttt
    12541 ccataacata cgtttggatc ggataacgat tcgcaggcgg tgtttcgatt actgaaagat
    12601 cccgcacgcc taacatcgac atatgcaaag ttctcggaat cggtgttgca gtcagagtca
    12661 aaacatccac ttgtgcacgt aactgtttca atcgttcctt atgttttaca ccaaaacgtt
    12721 gttcttcgtc aatgatcagt agacccaaat cgctaaattc aatatctttt gacaaaattc
    12781 ggtgggttcc tacaacaata tcaacttgac ctgtacgcag ttgttcgatg gtttcttttt
    12841 gttgctttct tgttctgaaa cgacttaata gcccgatatt tacggggaaa ccttcaaatc
    12901 tttccagcat cgtttcatag tgctgctgtg ccaagatcgt tgttggaact aaaaaggcta
    12961 cttgtttgct ttcctttatc gctttaaagg ccgctcgaag cgctacttct gttttcccat
    13021 aaccaacatc acctacgagt agtcgatcca ttggtttttc tttttccata tcccgtttga
    13081 tctccgctgc acttctcaac tggtcatcag tttcagagta ggggaaggca ttttcgaatt
    13141 ctttttggta ggcgtcatca ggtccaaaag catatccttt ttctgactct cttgcggcat
    13201 ataatttgat tagatcgtca gcgatatcct cgatttttga agagactttt cgcttggttt
    13261 tcgtccattc acttccgcct agcttattga tgcgaggcgc tttggcttca gaggcgacat
    13321 atttttggat cagattcagt tgagtaacag gaataaacag tttgtcgtcg ttttgataca
    13381 aaatagtcat atagtcttgg tgaacgccat ctacttctag cgtttccatc ccaatgtatt
    13441 tcccgatacc atgattggca tggacaacgt aatctcctgc ttttaactca ttatagcttt
    13501 tcaatcgctc tgcgtttgtt actgtttgac gtctcgtttg ttttttcgtc gttttttgga
    13561 aaatttcttt ttctgtgata gctactattt tttcttgagg aagttcaaaa ccagcctgca
    13621 aagctccttc aactaattgt acctgtcctc tgatcaatgc atttttttct gtttctacta
    13681 ctaatatgtc ttcatctcga agcatttgtt ctgctttgcg tattctgtcc tttgtcggaa
    13741 taaagatcac aacagtctgt ttttgcttgc gccaacggtc catttctgtt ttcagcaatg
    13801 gcatttgtcc gaagaactgt tgcatcgtcc gatattgaaa attatggatt gcttggaaac
    13861 gaagatttcc catccctttt tggaagagtg agaaaaaggt tgtggtgaac ttttccttct
    13921 gcacgatttt ttgtatatct gctgaaaatg tctgctcaga aaacacccgc aattcgctga
    13981 ttttttgagt ctgccattct gccgcttccc tttcgatttc ccgatttgtt tccatgattc
    14041 gctgataatc atcaacgact agtaaactat ttgaagggaa ataatccaag atcgtcacag
    14101 attcttggta aagaagatct gagtagaatt ttgcttcttc tgtcgggaca cctgcttgcc
    14161 agcttgaagt cagctgaccg aagtaatctt gcaaaaactc gcggtctgct tcttctgtgg
    14221 tcgcagcaag ccgtttttct agcatttttt gcatttgctc tattccatgt tctagatcct
    14281 ctggtccgta gacttgttca ctcgttgggg aaacccagat ttcttccgtt ttttcgattg
    14341 agcgttgtgt ttctgcttca aaataccgca tagaatctat ctctacatca aatagttcta
    14401 cacgaacagg ataggttgtc gttaacgggt aaatatcgat aatgcttcct cgaatactga
    14461 attctcccgg ttttgcgacc atctcttctc gtacatagcc catcaaagtc aattggcgag
    14521 gaagattttc cacgtctatc tcatcaccaa tatgccaatt caattgcgcc tgtagccaag
    14581 ttttcttatt cggcagtcgt ttgtgcaaag atgcgactgg caacaaatag atacctgctt
    14641 ttttttcttg aagtgcgtta agcgttgcaa ctctctcgcc tcgcgcttct ggagaagaaa
    14701 aggccatttc agcagaaagc acttcatcca ctggaaataa atggatctga tcttgcggaa
    14761 tcacatgttg cagatcttct gctaactgat tcgcataata caaatttggc atcagaataa
    14821 tcatgttttt atttgttttc ttaaacgctc gagcaaatag aagtgttttc gctgagcccg
    14881 ctaatcctgt gattagttgt ctaggcattt tttcttctaa attgctgacc cattctttta
    14941 cttgcggatc ttttccgact aattgaataa tatccattcc ttttcctcct tcctcaattg
    15001 aatcgattca tcgcatccac gaacgaatgc ccttctatga gatattcgac tgctttaaca
    15061 gattgacaca tgctttcttc tatcaaaggc tgattttctt tgctgaaagg actaagcaca
    15121 tgctggacta ctgttttttt cccttctggc ctgccgattc ccaccttgat tcgatcaaag
    15181 acattcgtat tcaaatggga aataatactt ttgataccgt tatgcccgcc ggcacttccc
    15241 ttttgacgga gccggatttt tcccactgcc agatccaaat catcgtagat tacgactaat
    15301 tcctctggat aaatcccaaa ataagtcatt agtgggccga ctgcacgacc agattcattc
    15361 atgaatgttt gtggtttgac taaaaggatc ttttctccat tatgaaaaaa ttcggcaact
    15421 tctgcttcga acggattttt tttgaaagta gcttgatgct cgcgtgcaat tttatccacg
    15481 accataaagc cgatattgtg ttttgtatat tggtatttgg tacctgggtt tcctaacccg
    15541 acgatcattt tcatttttgt tttccaccta attctaaaaa gcagcacaaa aggctggact
    15601 gctaattagc ggtccagtcc atcgagcttt tttctttttt atacttgtca atgcatagta
    15661 taacacaaaa caaggcgttt cggctaaaag agaatcgtta ataaatgata aataaattaa
    15721 aaatttcttc aaagtttaat gaaataattg atatataaaa atagtgaaat aacgcacatg
    15781 taagctttta catataatac aaccttgtta tctgttatat tttatagcca aatagcgtga
    15841 catggggctt gagaaatgtt agcataagca tgggaaaaaa caaatcaatg gaaggattga
    15901 tactaaatga ctgcagctgc agaaaaaaaa gatcatcaaa aagttattct tgttggagac
    15961 ggcgccgtag gttctagcta tgcctttgct ctagttactc aaaatatcgc tcaagaagta
    16021 ggtattgtgg atatcaatac tgctaaaact gaaggagacg caattgactt gtctcacgct
    16081 ttggcattta cttcacctaa aaaaatctat tctgctactt acgcagacgc acatgatgct
    16141 gacttagttg ttattacagc tggcgcacca caaaaaccag gtgaaactcg tttagactta
    16201 gtaaacaaaa acctaaaaat caaccgcgat gttgtcacac aaatcgttga atcaggattt
    16261 aacggtatct ttttagttgc cgctaaccca gtagatattt tgacttactc aacttggaaa
    16321 ttctcaggtt tccctaaaga acgcgtaatc ggttcaggta cctctcttga ctcagctcgt
    16381 ttccgtcaag cacttgcgga attagttgac gttgatgcac gtaacgtcca tgcttacatc
    16441 ttaggtgaac acggagattc agaattccca gtttggtcac atgcaaatgt tgccggcttg
    16501 caaatctatg aatgggtaaa aaacaaccca gaaatcgatg aagaagctat ggttaaccta
    16561 ttctttggcg tacgtgacgc tgcttacaca attatcgaga aaaaaggcgc tactttctat
    16621 ggtatcgcag tagcattagc acgtatcact cgtgccatct tagatgatga aaatgctgtt
    16681 cttcctttat cagtattcat gaatggcgaa tatggattga acgacatcta cattggtgca
    16741 cctgctgtta tcaaccgtca aggtatccaa aaagtaatcg aaattccttt aagtgacagc
    16801 gagcaagacc gtatggctgc ttctgctaag caattaaaag aaattctaga tgaagctttt
    16861 gctaaactag acgctgaata atctttgaac atgacggaaa acatctattc ggtgttttcc
    16921 gtttttttat tttatcaccc ttatggtttc taaatttttt cataaagtag agataacctt
    16981 cgtcttttcc tctaaattat gctatcatga gaccgttcca ccctaaactt ttacttaagg
    17041 agttgtcgat atgacaagat cttcgcatcg aaaaaaatcg aaacatgttg ctttgctttc
    17101 agttcttggt gttctcttag tgctgatcag cttttatacc tatcgcagta catactacac
    17161 aaagcatttt ttgccgaata cagaaataaa tggaataaat gtcagcaatc taacagtcga
    17221 aaaagcaaat gaaaaattaa aagaagctta ttctgataaa aaactggcta ttaaagaaga
    17281 tggaaaagtc tggcaggaag taccaaaatc cgagctcggc tacaaagatg atttcacttc
    17341 ggagctttct ccgatcctaa atgaacaaaa cggatggact tggggaatga cttatgtctc
    17401 agcggcagaa aaacaagaaa tagatcctgt cagtcaaaac caccaaaaac tagacactac
    17461 cgttcagaca ttgacaacta agctgactga tttgaacaaa gaccggacac ctactactga
    17521 tgcaacgatc gaaaaagcag gtgactcttt cgttatcaag cctgaagtca atggaaatac
    17581 gatcgacgtc gatgctgcgg tcaaacaatt aaaaagtgcg gtaaactctg gtaaagatac
    17641 aattgaattg actgaattca aagaaaaacc aaaagtaaca tctgaagaca gctcactgaa
    17701 agaacaactc gcatccatga atgctatcgc aaatgtaaaa gctacttaca gtataaacgg
    17761 tgagactttc cagatcccat caagtgatat tatgagttgg ctgacctaca atgatggaaa
    17821 agtcgattta gatacagaac aagtacgtca atatgtaaca gatttaggca caaagtataa
    17881 cacaagtacg aatgacacaa aatttaaaag tacaaaaaga ggagaagtta cggtcccagt
    17941 tggcacttac agttggacga tccaaacaga cagtgagaca gaagcattga aaaaagcgat
    18001 tctggcaggc caagatttta ctcgttcacc cattgtgcaa ggtggcacaa ccgctgatca
    18061 tccattgatc gaagatacgt atatcgaagt tgatctggag aaccagcata tgtggtatta
    18121 caaagacgga aaagtggccc ttgaaacaga tatcgtttcc ggaaagccaa ctacacctac
    18181 acctgctgga gtcttctatg tttggaataa agaagaagat gcaactttaa aaggaacaaa
    18241 tgacgatgga acaccttacg agagtccggt aaactactgg atgccgattg actggacagg
    18301 tgttggtatc cacgattcag actggcaacc tgaatacggt ggagacttat ggaaaacccg
    18361 cggttcacac ggctgcatca acaccccacc aagtgtaatg aaggaattat tcgggatggt
    18421 cgaaaaagga acgcctgtat tggtcttcta acaaaaataa tatataaaaa gaaagcaccc
    18481 gtacgattgg tacaggtgct ttctttttat tatctaacaa tgtgctactt acatttcagc
    18541 agcagtaaag acttctgaaa catcatcatc gtcttctagc ttatctacta gacgttccaa
    18601 ttgctctttt tgttcctcat ttaaatcaat cgtcgtttgt ggaatcatcg tcaattctgc
    18661 ttgcgccaaa gtgaactctt tttccaactc atctcgtaca gcagcaaaat cttctggtgc
    18721 agtataaatc tcgaatactt caggagaagt ttgcagatct tctgcccccg cctctaatac
    18781 atcctcaaac atcgtctctt catcaacagt caacccatcg cgttcaatag cgatatatcc
    18841 tttgcggtca aacaagtaac tgactgaacc cgtttctccc agtgatccac cgtttcgagt
    18901 aaaagctact cggacatttg tcgctgtccg attacggtta tctgtcaatg catggactaa
    18961 gattcctact ccacccggac catatccttc atacgtgatt tcatcgtaat tcgtttcatc
    19021 tacggcactc gtagcctttt taatcgcccg ttccacattg tcgttaggca tatttgcagc
    19081 ttttgctttg tccacaacaa gccgcaaagc tggattcata gatggatcag gaccgcctgc
    19141 tttagctgcc atataaattt cacgagaaag tttctgaaag actttccctc gttttgcatc
    19201 ttgggcattt tttcttcctt ggatattatg ccatttacta tgtcctgaca tgatttttcc
    19261 tcccttgttc ttttgagaat acacttctat tctagtacta tttcacaaaa aaacaaagcc
    19321 acagccaatc tataaacgct tcttgaatgc cgcaaaaagc acacataata tcacagcagt
    19381 cagagcaccg atcgaatcca acatgacatc ttggaacata ggtgttcgtc caccggtcat
    19441 catctgatgg aattcgtcca aacctgcgta ccctgttgct gaaagccaag caaggatcgc
    19501 tgtcagccac caaattttta gcttggggaa aacacctaaa aacaagctgc ctcccaaaat
    19561 aaaataggta ccgaaatgtg cgcccttcct taaaaagaac tctaaaaatt tggcgtagcc
    19621 cagatggtcg atactcacca tacttccacc ataattaaaa cgtatacccg ctaatgtgtc
    19681 tttaaatggc tgattaggca gccagctttc taatcgcgaa acttgtgatt gctgtccgta
    19741 agtttgagaa gaactgtaaa ataagattat aacgactatc agtgcgatta ctaaataaat
    19801 atttccgttt ttcatttgtt tttttgaaga attcataagt cacctctgta ttttatctta
    19861 gacttttttt gaagaaacaa caatcatttt catagagact tcttccataa aaaagtgcta
    19921 tacttaattt tgtattacaa tatgaaggga gttttttcat gagttattca ttaaattgga
    19981 atctagattc tattttttcc ggaggcagtc attctgatgc tttgaaccaa cggatgaagc
    20041 aattggaaga ccaaatgaac gagtattatc accgcgtaac aaaatggtca cctagttctg
    20101 acaatgcaga gcagttaaac gctattcttc aattgcaaga aacgattacg aatggtttta
    20161 cccaatgcag cagctatatt actgcattgc tttctgccaa tgtcaatgat tcagatgcaa
    20221 aggtcttatc aggcaagctt tacgcaatgc tgcctcgtct gcaatcagcc gaaactgtat
    20281 tgtcaaagaa atttgcagaa atatctgaca atgattggaa tcagatgctt tctcaatctt
    20341 cgtttgaaac aattgctttt cgtttaaacg agattcgtcg tgatggctca caattattat
    20401 cagaagctga agaaaacatt ataaatacgc tttctttaga tggtctaaat gcttggagca
    20461 gtcattatga tacgattgta gccagcattt cgattccctt cgaacaagat ggacaagtag
    20521 tcgaattatc tgctggacaa gccttcaata agatgatggg cgatccagat ccaaaagtca
    20581 gagaaacact tttcgcagca tgggaagaag cctgggaaga aaaggcaccc ctcttcacgg
    20641 atactttgaa tcatttagat ggttttagac tttctgacta tgaacttcat ggtgtcacag
    20701 attttctaca aaaaccatta gagtacaatc ggttgaagaa agaaacattg tctgtcatgt
    20761 gggatacaat ccagaaaaac aaacaacccc ttgttaatta cttgactaga aaagctaatc
    20821 ttttcggtaa agaaaaaatg gaatggcaag atcaagatgc acctattatt ttaggcgatt
    20881 tgaaagaaaa aacctttagt tttgacgaag cagcagcatt tattatcgaa aactttaaca
    20941 aattcagtcc aaaaatggct aaatttgccc aatctgcttt tgaaaagagc tggatcgaag
    21001 cagaagatcg tcctgggaaa cgtcctggtg gttattgtac tgagttacca gaaacaaaag
    21061 aatcaaggat ctttatgacc tacagtaatt ctgttaatga ggttgcgaca cttgctcatg
    21121 aactaggaca tgctttccat agcagcacga tgtgggattt accttcttta aatcgcgaat
    21181 atgccatgaa tgtcgcagaa acagcaagta cattcgcaga actgatcgta gccgatgcta
    21241 cattaaaagt cgcaaaaaca aaagaagaaa aaatcaattt gctagataca aaactgcaaa
    21301 atgctttagc tatgttcatg aatatccatt cccgattcat ttttgaaaat cgattctatg
    21361 aagcacggca agaaggatta gtctcagaag ataagatcac agagatgatg gttgaggcgc
    21421 aaaaagaagg ctatcaagga gctttagcta cttaccatcc atatttctgg gctgcaaaat
    21481 tgcatttctt tattgatgac gtaccgtttt ataacttccc atacacattt ggctacctct
    21541 ttagtttggg catctatgct tacgccaacc aaaagggagc aaattttgaa gaagaatata
    21601 tccatttact acgcgatact gcctcaatga cgacggaaga tcttgcgaag aaacatttga
    21661 atgtcgatct tacccaacca gatttttggc aagcaggtat cgacatggtg actgaagata
    21721 tccaaacgtt catggaattg acagaagaat ttatctgatt tctattttat cacaataaaa
    21781 aa
//
LOCUS       DACEUF010000044        21462 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.045, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000044 DACEUF010000000
VERSION     DACEUF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.045"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            68..808
                     /locus_tag="IXG20_001613"
     CDS             68..808
                     /locus_tag="IXG20_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1609814.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="IXG20_001614"
     CDS             complement(1232..1402)
                     /locus_tag="IXG20_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1609815.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="IXG20_001615"
     CDS             complement(1596..1775)
                     /locus_tag="IXG20_001615"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609816.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2162..3274
                     /locus_tag="IXG20_001616"
     CDS             2162..3274
                     /locus_tag="IXG20_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1609817.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="IXG20_001617"
     CDS             complement(3352..3645)
                     /locus_tag="IXG20_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ1609818.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="IXG20_001618"
     CDS             3813..4127
                     /locus_tag="IXG20_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ1609819.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="IXG20_001619"
     CDS             4148..6127
                     /locus_tag="IXG20_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1609820.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="IXG20_001620"
     CDS             6140..6466
                     /locus_tag="IXG20_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ1609821.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="IXG20_001621"
     CDS             6482..7000
                     /locus_tag="IXG20_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609822.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="IXG20_001622"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="IXG20_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1609823.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="IXG20_001623"
     CDS             complement(8436..9941)
                     /locus_tag="IXG20_001623"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ1609824.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="IXG20_001624"
     CDS             complement(10033..10623)
                     /locus_tag="IXG20_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1609825.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="IXG20_001625"
     CDS             10802..12448
                     /locus_tag="IXG20_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1609826.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="IXG20_001626"
     CDS             12497..13216
                     /locus_tag="IXG20_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1609827.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="IXG20_001627"
     CDS             13695..14642
                     /locus_tag="IXG20_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1609828.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="IXG20_001628"
     CDS             14654..15574
                     /locus_tag="IXG20_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1609829.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="IXG20_001629"
     CDS             15615..17072
                     /locus_tag="IXG20_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1609830.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="IXG20_001630"
     CDS             17417..18031
                     /locus_tag="IXG20_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1609831.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYSSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="IXG20_001631"
     CDS             18024..19748
                     /locus_tag="IXG20_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1609832.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="IXG20_001632"
     CDS             19745..21196
                     /locus_tag="IXG20_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1609833.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6918 a         3644 c         4339 g         6561 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat attcatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgga gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
    21361 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc
    21421 tccactaaac ggtgttcaat cagctgaccc tcggtattag ct
//
LOCUS       DACEUF010000045        20286 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.048, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000045 DACEUF010000000
VERSION     DACEUF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.048"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            141..461
                     /locus_tag="IXG20_001633"
     CDS             141..461
                     /locus_tag="IXG20_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609834.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            913..1113
                     /locus_tag="IXG20_001634"
     CDS             913..1113
                     /locus_tag="IXG20_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1609835.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1260..4049
                     /locus_tag="IXG20_001635"
     CDS             1260..4049
                     /locus_tag="IXG20_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1609836.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4096..4623
                     /locus_tag="IXG20_001636"
     CDS             4096..4623
                     /locus_tag="IXG20_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1609837.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4644..5834
                     /locus_tag="IXG20_001637"
     CDS             4644..5834
                     /locus_tag="IXG20_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1609838.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5874..7172
                     /gene="asnS"
                     /locus_tag="IXG20_001638"
     CDS             5874..7172
                     /gene="asnS"
                     /locus_tag="IXG20_001638"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ1609839.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8225..8899
                     /locus_tag="IXG20_001639"
     CDS             8225..8899
                     /locus_tag="IXG20_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1609840.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8896..9237
                     /locus_tag="IXG20_001640"
     CDS             8896..9237
                     /locus_tag="IXG20_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609841.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9267..9626
                     /locus_tag="IXG20_001641"
     CDS             9267..9626
                     /locus_tag="IXG20_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ1609842.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10150..12231
                     /gene="cadA"
                     /locus_tag="IXG20_001642"
     CDS             10150..12231
                     /gene="cadA"
                     /locus_tag="IXG20_001642"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1609843.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12542..14008
                     /locus_tag="IXG20_001643"
     CDS             12542..14008
                     /locus_tag="IXG20_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1609844.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            14623..15162
                     /locus_tag="IXG20_001644"
     CDS             14623..15162
                     /locus_tag="IXG20_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ1609845.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            15236..15676
                     /locus_tag="IXG20_001645"
     CDS             15236..15676
                     /locus_tag="IXG20_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ1609846.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            15689..15898
                     /gene="copZ"
                     /locus_tag="IXG20_001646"
     CDS             15689..15898
                     /gene="copZ"
                     /locus_tag="IXG20_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ1609847.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            15898..18084
                     /locus_tag="IXG20_001647"
     CDS             15898..18084
                     /locus_tag="IXG20_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ1609848.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            18104..20275
                     /gene="copB"
                     /locus_tag="IXG20_001648"
     CDS             18104..20275
                     /gene="copB"
                     /locus_tag="IXG20_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ1609849.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT         6778 a         3527 c         4407 g         5574 t
ORIGIN      
        1 aaaaaagagg ttgtgacgtg gtcacgacct ctttttgacg attatgatac aaaaaacatc
       61 atttatgaac aaaatgtgaa taacgggcgt tcttgagata tgattttaat tgggaaaaac
      121 ttatcgataa ggaggtcttt atgaaaaaaa gactgttatg gtgtttgctg tttctatccc
      181 taagtttaac aagtcagcca gtttttgctg aagaaacaca gcggtctcct attacaggca
      241 cacgtctgta caaaaatgtt tatgtacaga caacagatct tgattctgcg attcctgaaa
      301 cagaagaacg gtctttgcag ccaagatttg gttacttttg gtactgtccg acctgtgatt
      361 atgaaagttg ctggcatttc ttacattcta cagctgcagc ttttgctttt actcatactc
      421 agaaatatga tggacatcat cctgttattt atgaggtata gatatgagca aaaaagaaaa
      481 actaatctcg ttgatcgtat tggtttgcct tgttcttctg gtaactgtca taggctatca
      541 gaaataccat gagaacatca gacctactaa agaatcttcc ccagcctcga ccgttgaaac
      601 agaaccgata agtagtcaga aagaataatt tttttagttt aaagaagaac atcagagcaa
      661 aaaaacacca actgaacaat atgtcgttta ttttaaaaaa agttcggttt ttcactttat
      721 gtaatcgctg agaaaacttt ttttaataaa aaaagtcgaa aaactctagc ttttttgtaa
      781 acggtatgat atactatagg agtagttttt gttcggtaat ctgtggtagc ttgtttcaaa
      841 taaacatctt ccagatatac accattcaag taattgcaaa atatgtgttg atacacaagg
      901 aggattttta ttatggaaac aggtacagta aaatggttta actcagaaaa aggatttgga
      961 ttcatcactg cagaaaacgg aaacgatgta ttcgttcatt tctcagctat ccaaggcgac
     1021 ggattcaaaa ctttagaaga aggccaagca gtaactttcg atgttgaaga aggccaacgc
     1081 ggacctcaag caacaaacgt tactaaagct taattaacct atactaaaaa ataaacgccc
     1141 agacatttgc gtgtctgggc gtttttgtct attttcttta aaaagcgatg agagtatttt
     1201 tctgcacttt gtagtataat ttcaaaagaa cattagaggc aggagaagag gaagatattt
     1261 tgaaaccaag aacttatgca gtcgttgata tcgaaacgac tgggacgaat ccaaaagaag
     1321 atcggatcat ccaatttggc tgcgtcatga ttgaatcagg tcggatcact tcacgttttt
     1381 cgatcgatat tcatccaggg cggaagattt caaaacaaat ccagcattta acagggatca
     1441 cgaatcaacg cgtccaaaaa gcgccttatt tcgaagatgt cgcccaaacg atttacaatc
     1501 ttttggcaga caccatattt gtcgcgcata atatttattt tgattataat tttttgaacc
     1561 atgaactcat gcgatgcgga cttcctagtt tgaagatacc aggcatcgat acagtagaac
     1621 ttgcacaaat tttcttgccg acggaaccaa gctttcgctt agctgatttg tcagaaagcc
     1681 ttggatttat tcatgagaat cctcatcagg cagatagtga tgccgaagtg acgggtcaac
     1741 ttcttttgct gatcgaagaa cggatgagaa aattgccaat catcacgtta gagcagattg
     1801 cgcgattaag tagacacaca ggtatggata cgagtcgttt catttatcat gtgattgaag
     1861 aaatgaaaga aaaaccagag ccacttgatc cctcattaga aatcgtcgat gggctagctt
     1921 tgcagaaaaa agaagtggaa ctattcacat ccgtccatta tggcgagcgg acatatccaa
     1981 gaaaaaaaca agcaaaagaa aaaatgtttg gtaaaacact gatgtatcga aaagaacaga
     2041 acagactgat gaatgcggtc tacgaccatt tcacaaaaga tgaatcaaaa gatttgatga
     2101 tcgaagcagc tacaggaatg ggtaaaacaa tcggttatct gctgccattg agttatttgg
     2161 ctacacctga aaaacccgct gtcatcagta ccgtttcgct agttttgcag cagcagatca
     2221 tagaaaaaga cattccattg ctgaatcaat tgctagatca gcccatacaa cctgtaatta
     2281 tcaaaagcta tcgccactat attgatttac agcgttttaa aggcacactt gtggaaccgc
     2341 cagaacaaaa acaatatgct ttgtatcaaa tggcggtctt agtttggctg acgcaaacga
     2401 agaccggtga tttggatgaa ttacatttaa caaatttgaa ccattcattc tttgcagata
     2461 tcgcgcacag aggtattgga tttttagcaa gaaatcagtc attttacgaa caagactttg
     2521 ttcgtttctt gcaaaagaaa atcaggcaaa gtaatttttt gattgtcaat catgcgtttt
     2581 taatacaaga aacacagcga aaagaaccat tgcttccagc tagtccattt ttgttgatcg
     2641 atgaagtaca tcatttgccg gaaatcgctg aaaaagtaaa tgacaaacga ttaagtatta
     2701 actatttcca taaacaaatc cagcaattag aagaaccaaa tcagctgttt gaccgaatac
     2761 aaagctattt accgaaagaa catgaagcct tacgctggtt atcgatttat cgagaagaac
     2821 tttctgcgct atgcgaagca cagatttgta tggcagaagg attaatgagt ttagtgagcg
     2881 gcaagcaacc taacggacag tatccagaag aagtcatgat gacaaaagag atgttggatc
     2941 agctgccact ggaatctggg acagccattg atcacctgca gcttttttat caggagatcc
     3001 agcagctaca gtcacaaatg cgtctgactt ttgagattat ccaagaaagc tggacaaacc
     3061 agatgcggat cgatttagca agtttatata gcttatttga tcaattagac aagcagtact
     3121 tgctgatgga tcaatggctg gaagactggc gagatcattt gatccattgg ttcgttcctg
     3181 ggaacacagc gcagcagatg atttttcatc tccatgattt tcaagcggct atgttgccgt
     3241 cgactgtttg gtatcctcga tatgatcgta ttctttatat tggaggaacg ctgaagatcg
     3301 gccctaatcg acactatctc tctaagcggc ttggcattga agcagcacct cttaaagtga
     3361 ttgcctctcc ttatgattat gagaaacagg caaggatcta tgtgccaaaa gaggcacctg
     3421 ccgtccatca agtcagcaat caagcatata tcgattattt agagacagca cttacaggtc
     3481 tgatcgaaca agaagatcgg ccaatacttg tgttattcac ttctcatgat gttttgcaga
     3541 aggtctatca gaaaatgcat ctgtctatgc tagaaaaagg acgagaaata ctagctcaag
     3601 ggtttggcgg aagccgggag aagcttttga aacgcttcat tttgtcagaa aaaagcgtat
     3661 tgtttggcgc tgacagcttt tgggaaggtg tagatttgcc tggggatgct ttgcagatcg
     3721 ttgtagtcac acgtttaccc ttcgataatc cgcttcgccc gatggtcaaa gctaggaacg
     3781 cttatttaga atcagagggg atcaatcctt tttaccagga atccgtacct aaagcagctt
     3841 tgaagttacg ccaagcatta ggtcgcttga taaggacgga gacagatcgt ggcgtattgc
     3901 ttattttgga tcgaagacta gtcactgcaa aatatggtgc acggatcatc catgcattac
     3961 cgaaaaaagt accgatcaaa gagctagcta tggaggaaat ctatcaggac atccatgaat
     4021 tcttaaagaa aaatgaagaa ggaagttaga ggagctgtct tttttcataa ttaaaatctg
     4081 ctataatgaa aaggaatgaa gataaaggag cgacgaccct tgaagaaaaa acagacaaag
     4141 gaaaaaacga ttaatcgcgt gttacttggc ttgatcgtat ttttattagc ggtcatcgtg
     4201 atcagctcga ttttttactt gcgttccagt cgtccggcgg tgcaggcgaa gaaagaagca
     4261 gtagaaatcg caaagaaata tgcaaatctt gaggaagccg atcattttta ttggtttact
     4321 cgaaagaaga catacttttc actgacaggt acaaatgata aaggaaaaga aatcgccgtt
     4381 atcattccta aatctggtga aaaagtaaaa gtattggatc aaacaaaggg actgactgaa
     4441 aacgaagcaa aacagacaat tgctgcggcc catcctgaaa tccaaatcga aaaagctgct
     4501 ttagggatgt atgatgatca gcctgtgtgg gaagtagtag gaaaagaaac aaatgggaaa
     4561 ctgaattatt acctgattgg ctttgaatct ggagatgaag tcaagacaat caagggaatt
     4621 taaaaggtaa aggaatgaat gggatggagt tatcaaaaag aacaaaacga ttagaaccgt
     4681 ctgtcacttt ggcagctgcg gcaaaggcca aggcattaaa agcaaaaggc agagatgtgc
     4741 tgagtttgac tgtgggagaa ccggattttg cgacaccaga aaacatccaa gaagcagcaa
     4801 tcgaagcaat ccgcaacggg aaagctagct attatacacc aacagcagga atcccagagc
     4861 ttcgacaggc aatcgttgat tatctgaaga aaaactatgg acttgtctat gaaccttcac
     4921 aaacagtcgt aacagacgga gcgaaatttg ctttgtatac ccttttccaa acgatccttg
     4981 acccgcaaga tgaagtcatc attcctgtgc cttactgggt cagttacggt gagcaagtaa
     5041 aactggcaga aggacttcct gtctttgtga aaggggaaga atccaatagc tggaaagtta
     5101 ctgttgaaca gctagaacaa gcacgttcag aaaaaacaaa agccgtaatc atcaattctc
     5161 catcgaatcc gactggaatg atttatagca agaatgaact gcaggcaatc ggtgaatggg
     5221 cagtaaaaaa tgatattttg atagttgctg atgatattta tggtcgttta gtttataatg
     5281 gcaatgagtt tactccaatt gcaacgattt cagaagcaat caaaaatcag actatcatca
     5341 tcaacggcgt gtctaaaaca tatgcgatga caggctggcg cattggttat gcggtgggaa
     5401 atcctgtgat catcaatgga atgatcgctg ttgcttccca gtcaaccagt aatccgacag
     5461 cagtcagtca atatgcagct gttgaagcat tgaaaggcga gcaagataca gtggaagaaa
     5521 tgcggaacgc ctttgaagaa cgcctgaata cgttatattc gcttgtggca gaactgccgg
     5581 gtgtttctct tgaaaagcca caaggagctt tttatctttt tcctaatgta aaagaaacgc
     5641 tacgcatgtg taaatatgaa aatgtcacac aatgggtaga agatctttta gaagaaacag
     5701 gggtagcgct tgtgactggc gaaggctttg gggcaccaga aaatgtccgc atgagttatg
     5761 ctactgatcg cgctacgtta gaagaagcgg tacgcagaat caagcaattt attgagagta
     5821 aaagtcaaaa ttaatcctaa agaaattcaa ggaatataaa aggagagtca tacgtggaac
     5881 aaattcaaat catcgattcg aaaaaccatg tgggagaaac agtaacaatc ggcgcttggg
     5941 tcgcaaataa gcgatcaagc ggaaaaatcg catttttaca attaagagac ggaacagcct
     6001 ttttccaagg tgtagttgta aaaagcgaag tgtcggaaga agttttccaa acagctaaaa
     6061 atctacacca agaaacatcc gtgttagtaa ctggagaaat ccgtgaagac agtcgttcaa
     6121 aatttggtta tgaaatcgga attacaaatg tggaagtggt aagcgagagc cacgattatc
     6181 cgatcacacc aaaagaacac ggaacagact tcttgatgga tcatcgtcat ttatggctgc
     6241 gttcttcaag acaacatgcg attatgcaga tcagaaatga aatcattcgt gcaacgtatg
     6301 aattctttaa tcaacatcat tttgtcaaaa ttgatccgcc gatcttgact ggttctgcac
     6361 cagaaggcac tacggatcta ttcgaaacaa attactttga tcaaaaagct tatttatctc
     6421 aaagtggcca attatacatg gaagctgctg ctatggcttt cggaaaagtc ttttcattcg
     6481 gtccgacttt ccgtgctgaa aaatcaaaaa cacgacgtca tttgatcgaa ttctggatga
     6541 tcgaaccaga aatggctttc actcatcaag aagaaagttt agaagtacag gaacaatatg
     6601 tagcattctt agtacaaaat gttttagatc attgcgaata tgcattagat gtactaggac
     6661 gagacaaaga agtcttaaaa acttatacaa aattgccatt ccctcgtatt acttatgatg
     6721 atgcgatcga attactgaag aaaaatggat ttgatgacat cgaatggggc gaagactttg
     6781 gttcacctca tgaaacgttc attgcgaatt cattcgaaca gcctgtcttt atcctaaact
     6841 atccaaaagc catcaagcca ttctacatga aaccacatcc aacaagagaa gacgtggtga
     6901 tctgtgcaga tatgatcgca ccagaaggtt acggtgaaat tattggcgga agcgaacggg
     6961 caacagacta tgatttcttg ctagaacaaa tccgtaatca cggtttggat gaaaaagaat
     7021 attcatggta tcttgatcta cgtaaatacg gttctgttcc gcactctggt tttggtctag
     7081 gtcttgaacg aacagtcact tggattgctg gtatcgacca tgtacgtgaa gctattccat
     7141 tcccacgttt gttgaaccga atttatccat aagaggtgat gaaaaagctg ttctgcatca
     7201 agtaattaga acagccaagc aaaaacagct gttcatgttt ttgaattggc gggacttatt
     7261 accgcagatg taagcttttg aataccgttt agtggagagg ttgtgaaaaa gctgtttagt
     7321 ttcgagtatt aagaaaaatt ttgtggaaat ggctttccac atttttgcat aaatttggct
     7381 taataccgag aaactggctt ttgaacaccg tttaaggaag aggttgtgcg cctgccgttc
     7441 agctccgagt cacaaggagt aatttcaaaa aacagttttt catgtttgat gactattgtg
     7501 acttgtgacc gaggagttgg ccggagagca ccgtttattc taaaaaagaa ctgcgaactc
     7561 tcgcagttct ttttttaaaa tacgggataa aggaagtttc cagcagacaa gagaagaaag
     7621 caaaagaaat tgctatttga ttcacaaggt tctataacga aatcgcaatc aatcaaacat
     7681 ttacaaagaa ggtaattcaa atggaaatct gatttgatga tgcttctgct gttgaaataa
     7741 agcagcatct agcacctgaa aacaaactaa tagtaacatt cgaagatgga gtaggtccgt
     7801 attttcagca tgcaataatc catatgtaag ttcaattcac ccttgaaatc atcggaccag
     7861 ataaagttat gaccaatcta tcccaagcaa tctagaagag atattagtaa agggacattt
     7921 actagaagtc ttagatgagc ctataaatat atgttctgat ggcgtcatca gtggtctgat
     7981 tgacggaagc ttaggtgtga tcgatctctc tggaccaaac aacgaaggaa tcaaggggaa
     8041 tccggcacgt taagaaaaaa atagcataaa aaactagaca cttgatgagc gttcattcaa
     8101 ttgtttgaag ggactaggct ggtgtcattt tttatacata tattttctct ttcgtcttga
     8161 tagggcttat gggataatat aaggaagaaa atcatcgttt tattcaaaaa ggagtttttg
     8221 acaaatgaaa aaacgtatat tatttatcgg tgatagcatt acagacgcag gaagagaccg
     8281 agcagaagca actgatctag gtaaaggtta tccacttttg attgccggaa aactaatctc
     8341 ccaatatcca acgatggaat ttgaatgcat gaaccgtggg ataggaggag acaaaattgc
     8401 agatctgcaa gtacgctggc aagaagattg tttagcttta caacctgact atgtatcgat
     8461 tttgatcgga atcaatgata cctggcataa tactagcgat caggctatat tcggaactga
     8521 gaaatctgct cagcaatttg aatccgttta tcgaaaatta ctaaatgaac ttcaaacagt
     8581 gggaatctgc aacatcatct tgatggagcc gtttgtactt cctataaccg acgaccgtca
     8641 tggctggcgt aaagacttag accctaaaat ccaaattgtt cgtaaacttg ctaaggaata
     8701 tggcgcattg ctggttcctt tagatggtat attcaatgct ttagggattg tttatggttt
     8761 ttcgaaatat acaagagaag atggggtcca cccaacgatt ggcggacatg agatcattgc
     8821 acaaagctgg atagatgcaa ttgccgcagt cgattttttt agtggagata aaaatgagcg
     8881 aaagaaaagt gagaaatgat ggaaatacgg tgggcagtaa aagaagatct acctcaaatc
     8941 aatcagctga ttactgatca tccagaatca ttcgtaaaag caaaaacggc agctgaccta
     9001 aatgataaga ataaactatt gctcgttgct gtaacaaaag aaaaacggat tatcggattt
     9061 gcgcaagcgg acgtgcaaca gtcagaagaa cgattggatc agcgaccatc aggaaaacta
     9121 atccaacaca cattagtaac aaaagaaaat acggaagaaa tccaaaaaaa atttgcagaa
     9181 tatttgatac tggaagctaa aaaaagaaga ataaaatcat tttttgccca gttttaatca
     9241 aaaacagtaa tagaaggaga aatacaatga gttgtttagg aaatgtcatc tggtttatat
     9301 tcggcggact aatcggtggt cttagctggt tagcagcggg gatactttgg tgtatcacga
     9361 tcataggtat cccaatcggt atgcaatgtt ttaagatcgc ctccctttct tttgcgcctt
     9421 ttggcaaaga agtaatttat caccagtctg gcatgtcatt gattgttaat atcatctggc
     9481 tgatcgtttc aggcctacca attgctctcg cacatgtcgt cagcggcatc cttctatgta
     9541 tcacgatcat agggatacct tttgccaagc aatctttcaa acttgctcga ttggcgttga
     9601 tgccattcgg tgcagaaatt gtataaaaga gaataataaa ggttagataa agcatagagt
     9661 caggtgggat acatgtattt cacctgactc tatgcgttct cctgtataag ttcttccatg
     9721 tgacttttac gtcttttatt tatatggggt tactgtttat ggaaagttga aataattttc
     9781 attaaagaac tctttctata tttttgaaag cgtagtggag cttcgaatat ctgggaagga
     9841 cattttgcag ggattgaaaa aagatattgt cctatcgaaa aattaatgaa tttagacaaa
     9901 ttgccagcaa caggcttcaa acttagttgc tatcctgtca agatcaagaa ggcaagtgcg
     9961 ggttggataa gagcagtggc aatgatcgaa aagaaaaaga aagaataagc caaaacatct
    10021 gtatggaaga aattttccga tgcagatgtt tttcattttt tctttcggat accttgacat
    10081 atgaataacc gttcatttat aattagttat gaagagatat tcatatgttg aggcgaggag
    10141 gtcattaaaa tgaaaagtta cagattagaa ggtttagatt gtgcgaactg tgcaatgaaa
    10201 atcgaaaaag gagttcagaa gatcaatgga gtcaaggaag caacggtaaa cttcacttct
    10261 ggaaaactaa cgattgacgc agaggaagat catttggcta cgattgaaca agaaataaaa
    10321 aaagtagtga aggaattaga accagatgtc aaagtaacag aaatagataa ggaaaaagta
    10381 tctgaacatg gaaatgaaaa agagagaaat acgctgttcc ggattttatt ttcccttgca
    10441 ggtatcgctt tattgctatt atttgatttt aatgaaccaa ttcgcttgat agggtattta
    10501 ttgatttatc tgctgatagg atatgatgta gtgaagaagg cagtgatgaa tatcgtcaaa
    10561 ggaaaaatct ttgatgagaa ttttcttatg tctgttgcta cgattggcgc tatgttgatc
    10621 ggtgaatatc cagaagccgt agcagtgatg ctattttatc aaatcggcga gtatttccaa
    10681 ggattggcag tcagtcattc aagaaaatcg attcgcgaat tgatggcgat ccgtccagaa
    10741 acagctcacg tgcagacgac ggaaggattg atgaccgtga accctgaaga cgtgcagatc
    10801 ggtcagtttg tacttgtgaa gcctggtgaa cgtgtaccgt tagatggaac gatcatcgaa
    10861 ggagaatcat tagtcgatac gtcagcactt acaggagaat cagtccctaa atctgtatat
    10921 gttggtgaaa cagtattaag tggatttatc aataaaaata aaccattttt agttcaagta
    10981 gaaaaatctt atgaaaattc tactatcagc aaattattag aattagtaga aaatgccagc
    11041 agtaaaaaag caccagctga aaatttcatt actaaattcg cgcgctatta cacaccagtc
    11101 gttgtaggat tggccgtttt attagctgtt ttacctccac ttgttgtttc tggagcggcg
    11161 ttttctgaat ggatctatcg agcattgaca tttttagtga tctcatgtcc atgtgcatta
    11221 gtgatctccg taccccttag tttctttggc ggtatcggag gagcaagtaa aatcggtgta
    11281 ttagtaaaag gttcaaatta cttggagttg cttgcacaaa ctgagacagt ggtattcgat
    11341 aaaaccggca cattaacgaa aggtgacttt tcgatccaga cgatcgatac aaaaatcgat
    11401 ccaaagatat tcatgcaata tgttgcgagt gcagaacagt tttctacaca tccgattgct
    11461 caatctgttt tagaaggcta cgcaggtccg cttttaccaa cagctaatat ccaagaattt
    11521 gcaggtgaag ggatcttagc tgaagttgat ggcaaacaag tattagcagg taatcacaaa
    11581 ttgatggaaa gatttgaaat ctcttttcct tcaagtcaag aaatcggtac attgctttat
    11641 ctagcgatcg atcaaagtta cagtggttat cttgttattg cagatacctt gaaagaagat
    11701 gccgtagacg cattggttca attaaaaaag gctggtgtga agaacacagt catgttgacg
    11761 ggcgattcga aaaaaattgc tgatcatatc gggaagcaag taggcgtgga caagatatat
    11821 agcgaattgc ttccagaaga taaagtgcag cggttagagg aaatcttaca aagaaacaat
    11881 aaaaagaccg catttgtcgg agatggaatc aacgatgcac cggtgctagc aagagcagat
    11941 gtaggaatcg caatgggtgg attgggaagc gatgctgcaa ttgaagcagc agatgtagtc
    12001 atcatgaatg atcaaccatc gaagattgca gaagcaatcc atttagcaaa gaaaacattg
    12061 aaaatcgtca aacagaacat tgtctttgct attgggataa aaattttggt acttgcctta
    12121 ggtgcttttg gttttgcatc aatgggagat gcagtatttg cagatgttgg ggtgacagta
    12181 ttagctgttt tgaatgccat gcgaagcttg catgtaaaaa ataaacgttg atcaatgaag
    12241 aggacgaatg aacgtcctct tttttttata tccttttctt aatgaaaaat gtaaagaaaa
    12301 tagataggat tataaaataa ctaatgtttt taattttgca gaaaattcaa aatagcttga
    12361 aattcaataa ggtaaatgaa ataaaagaaa ataacttatt tttttgatgg aaaaaatatg
    12421 aaaaaacgat gtgtattaaa taaaagaata cacacataaa ataacactgt cttgtaagtt
    12481 tgttaaaaat tgaatatgat tacatctgga taacaaattt ttatcataaa ggagagaacg
    12541 aatgaacaac aaacaattac gctggtttac tgttgcgtta attgcattta acatggtttg
    12601 gggcatgggg aatgtagtta ataactatgc tcagcaagga gtatcagttg tcacctcttg
    12661 gctattgatt ttggccattt attttattcc atacgcattg atcgttggac aattagggtc
    12721 agccttcaag gatagtaaag gaggcgtcag ttcatgggtc gaaaatacga catctaataa
    12781 acgactggca tactatgccg cttggactta ctgggtcgtc catattcctt atttggcaca
    12841 aaagcctcaa gcaatcctga ttgctggcgg ttgggcagtc caaggaaatg gaaatatcgt
    12901 aaatacgatg tcagtacaaa tggtagcagt tatttcactt attatatttc ttgccttttt
    12961 atatttatca acaaaaggac tttctacttt gaaagtgatt ggcggactgg ccggaacagc
    13021 gatgttcgtc atgtcgatct tatttattct tttagctgta acggcaccgt tcatcaatcc
    13081 aacgatggaa tttgccacac cacatatgaa tcaagtgaaa acatatattc caacatttga
    13141 tttcagttat ttcacaaccg tttcgatgct agtatttgca gttggaggag cggaaaaaat
    13201 atccccttat gtcaactcta caagaaatcc tgcaaaggaa tttcctaaag ggatgatctt
    13261 cttagcagca atggtaggga tttgtgctgt attaggctca gtcgcaatgg gtatgctgtt
    13321 tgctagcgat aatattccta aagacttgat ggcaaatggg gcatacagtg cttttcaaat
    13381 tttaggtaat tactatggta tcggtaactc attgatgatc atttacgcat tgacaaacat
    13441 gattggacaa atctccgcat tagccttttc aattgatgca ccactaaaaa tcttgttagc
    13501 agatgcggat ccagaatttg taccaaactg gctacgtaaa cgtaatcgta aaggtacatt
    13561 ggtgaatggt tatcttctga caggtatact agtaggaatc attattttgt tgccgatctt
    13621 tggtatcaaa gatatgaatg aattagtaaa atggttgacg aacttgaact cagttgtcat
    13681 gccaatgcgt tacctatggg tgttcttggc atatatgatg ttgaataaag cttataagaa
    13741 attcaattct gaatataaat ttgttaaaaa tccaaaaatc ggttttatct ttggtgcttg
    13801 gtgctttgcc ttcactgcgt ttgcatgtat cctaggaatg gtgccaaaag tagattatac
    13861 tgctgatcct aaagcatggt ggttccaatt attcacgaat atcatcacac caattgtctt
    13921 gatcttatta ggtatgatct tgccagcaat tgctcgtcga gacaaaaaca aagaaatcac
    13981 tgaaccagaa acagtggttc agccttaaaa tagaaatact tgttacaaga ggttgtgaca
    14041 gaagtggtca gcttcaagaa ataagaagga atttacgaaa attgcttctc aaatttttgt
    14101 gaagtttcgg cttgtttttc gcagaagcta cttctggaac accgtgaatc agaggttgtg
    14161 aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagccc ctcatatttt
    14221 tcgttttgct gggcttatta ccgcagatgc aagcttttga acaccgttta agaaagaggt
    14281 agtgagcctg ccgtttaact ccgagtcaca aggagcaatt tcaaaaaaca acttctcatg
    14341 tttgttgact attgtgcctt gtgaccgagg tgttggcagg agaacaccgt ttaaggaaga
    14401 ggttgtaaaa tcagcgattt gacctgagta ttagaggaac aaacgaaaaa atagctcctc
    14461 atattttgcc gttttttgag ttaatgctga aggtcagctg attgaacacc atttattcga
    14521 aacaagggac tttgacacga cttttgtcac agtcccttgt ttttttgcgg cgttataagt
    14581 ggtatgatcg aaccaaattc caaagagaag tgagggagaa gaatgaagat catcgtgatt
    14641 ggacaaagtg ggtcgggaaa atcgaccctc gcaaggaaga tcaaagaaat caccgacttt
    14701 ccgctactgc cgctagacct tttatggcat acaacagact acagtattca ggcaaaaaag
    14761 tggtttttgc aagaacagca atcgtttatg gtatctaatc cgtcatggat tgttgaagga
    14821 aattatactt ctacattaga ggaaaggatt aaagaagcag ataagatcat ttggcttaaa
    14881 gtgccgcgtt atctcgccat ctaccgagta gtttggcgtt ctctaaaaag aaagataaac
    14941 aaaaaatctc ggccagatat gcctgaatca ttttcagaga aactaaacag ggaatactta
    15001 gaatttttat cttttatctg gcattttgaa gaaaacaatg aaccgaaaat acagcagctg
    15061 atccaagtag ctaatgcaag aaataaactg atcatcctta aaacaagacg agagaaacaa
    15121 gaatttttaa ctaaattgaa aatagagagc aaaaataatt gacagttttt gtttacaagc
    15181 gtaatctgta ttcataagct aatgattaca tttgtaaacg aaaggtgtga aataaatgga
    15241 aaaagtcaaa acgccagtaa agatcagtga ttcagagtgg gagatcatgc gtgttatttg
    15301 gacacttgga cagacgacag cccaagaaat cacgcagatc ttagctgatt cgatggattg
    15361 gaaaccagca acgatcaaaa ctttgctggg acgtttggta aaaaaagaag tgatctggac
    15421 agaacaagaa ggaaaaaaat tcatttatca tccagctgtc tcggagatgg aaacagttcg
    15481 cagtgcgacg gaaaatcttt tttcccatat ctgtgcaaaa aggattggag agacgattgc
    15541 ggatctagta gaagaagcca ctttgactca agaagatatc aatgagattt tgaaacaact
    15601 ggaacaaaag caaccagtag atacgatcga atgtaactgt atcccaggtc aatgtgaatg
    15661 taaacaacat caataggagg aagaaaaaat gaaacaacaa ttttcaatcg aaggtatgag
    15721 ctgcaatcat tgtgcagcaa gagtagaaga aacagtaagc gcacttgacg gcgtgcaaaa
    15781 agtgaaagta aatttgaaaa aagcaaacgg tacggtcaag tttgatgaaa caaaagttca
    15841 atccgaaaag atttgtcaag caatcaatgg attgggttat aaagcagagg tgatttgatg
    15901 gcaacagcag aaaccaaaat ggatacgttc gtcattacag gtatgacttg tgcaaattgt
    15961 tctgctagga tcgaaaaaga gctgaacgga caacctggtg tggtcaatgc cacagtcaac
    16021 ttggccactg aaaaagcaag tgtcaaatat gaagggacga ccactgaaaa attaatccaa
    16081 agcgtagaaa acatcggtta tggtgcaatt ttgtacgatg aagcccacaa acaaaaaatt
    16141 gcagaagaaa agcaagcgta tttgaaaaaa atgctattcg atcttatatt gagtacggtt
    16201 ttgactttgc cgttgatgtt gtcaatgatt gctatgatgt taggaagcca tgcggcgatt
    16261 gttcactttt ttcattttcc aatcgtccag ttagtgctgt ctgctcctgt ccaattttat
    16321 gtcggtgctc gtttttataa aggtgcgtac catgcaatca agacgaaagc accaaatatg
    16381 gatgtattgg tagcaattgg aacatcggct gcctttgcat tgagcattta caacggattt
    16441 ttccgtggac acccccaaga tctttatttt gaaagcagca gtatgatcat cacgctgatt
    16501 ttgctcggca aatatttgga acatacagca aaaacgaaaa caggtaatgc aatcaaacaa
    16561 ttgatgtctc ttcaaacaaa aaccgcccaa gtgataagaa acgggaaaga agaaacgtta
    16621 gcgattgaag aagtagtcgt tggtgatcaa ctagtcatcc gtcctggtga gcagattcca
    16681 gcagatggtc gaattatttc aggaagcagt gcgatagatg aaagtatgct tactggtgaa
    16741 aatcttcctg tagaaaagaa tccggatgac acgctttttg gcgggacaat caatacgaat
    16801 ggtctgctac acatggaagt cacacaagtc ggcaagcaga cagtattagc gcaaatcatt
    16861 caaatggtgg aagatgctca agggagcaaa gccccaatcc aaaaaatcgc agatcggatc
    16921 tcagggattt ttgttccgat cgtattagtc atcgcgttta tcacgctcat agctacaggt
    16981 ttgatcactg gtgattggca actcgcattg atccatagtg tctctgtatt agtcattgcg
    17041 tgtccatgtg cattaggttt agcgacccca acagctatta tggtaggaac gggtgttggt
    17101 gcacgaaatg gaattttaat taaaggcgga gaagcattag aagcagctgc tcacttagac
    17161 agcattgttt tggataaaac tggaacgatt acagaaggaa agcccaaagt tacagatcta
    17221 gtcggttcaa aagaagtcct atctattttt tatactttag aacaagcttc tgaacatcca
    17281 ctaggtaaag caatcgtgga atacggaaaa ctgcaagaag cagcaacata cgatatgatt
    17341 gattttacag cccacccagg tgctgggatc agcggtacga ttaatggagt acgctatttt
    17401 gcgggtacac gtaaacgatt aattgaattg aacctatcat ttgatgaata ccaagaacac
    17461 gcgttagaat tagaacagca gggaaaaaca gtcatgtttt tagctgatga aaagcaagtg
    17521 atcgggtgga ttgccgtggc tgatcaaatc aaactagaag ccaaacaagc aatcaagcag
    17581 ctccagaata aaggattaga tgtttttatg ctgacaggag acaacaaact ggcagccgaa
    17641 acgattggta aacaagttgg tattgatcca aagcatatct ttgctgaagt acttccagag
    17701 gataaagcag cctacgtaga aaaacttcag aaagatggca aaaaagtcgg tatggcaggc
    17761 gatggaatca atgatgcacc ggccttagca ttagctgatg tcggtatggc aatgggaagt
    17821 ggaacggaca ttgcgatgga aacagccgat gtgacgctga tgaatagcag tttggcatcc
    17881 attgcacaaa caatcgagtt gtctaggttg acattgagaa aaatcaaaca aaatcttttc
    17941 tgggcttttg tctataatac gattggtatt ccttttgccg cactaggctt tttgaatcca
    18001 atcattgcag gaggagcgat ggcgtttagt tccgtcagtg tattgttgaa ctccctgagc
    18061 ttaaatcgac acatgaaaaa gtaagagaag ggatggagta aaaatgaatg ggaacgatat
    18121 ggagaaaaaa catgagcata aaaaagaagt agtaaacaaa gaaagtacag aatctcatga
    18181 aaaacagatg gagatgaaac atgaccatag tcagatggat cattccatgc acatgggaca
    18241 taaccatgga gatatagcaa acagcaaaca aatggaccat tcgatgcaca tggatcacga
    18301 acacggcggc atggatcatt ccatgcacat ggggaatttc aaacaaaaat tttggctatc
    18361 gcttatccta gctatcccaa tcattctttt ttcaccaatg atgggaatgg aatttccatt
    18421 tcaagtaacg tttcctggtt ctgattggct cgtattgatc ttagcaacta tactatttat
    18481 ctatggcggt cagccatttt taagtggagc taagatggaa ttgaaacaaa aaagtcctgc
    18541 tatgatgaca ctgatcgcga tggggattac agttgcatat atttatagtg tctattcgtt
    18601 tatcgcaaat ctgctcaatc cacatacaca cgtcatggat ttcttctggg aattggctac
    18661 tttgatcgtt attatgctac taggtcactg gattgaaatg aatgctgtgt cgaatgccag
    18721 caatgcttta caaaaactag cagaattact gccagaatca gtgaaacgct tgacgaaaga
    18781 tggaaaagaa gagacagttt cattgaaaga agtaaacgaa ggtgatcgtt tgatcgtccg
    18841 gtctggtgat aaaatgccaa cagatggtgt aatcttgaaa ggtgaaacaa ttgttgacga
    18901 atcagctgtg actggcgaat caagaggaat caaaaaacaa gccaatgaca aagtgattgg
    18961 cggttctatc aatggggatg gaacgatcga aatcgaagta actggaactg gtgaaaatgg
    19021 ttatctagcg aaggttatgg aaatggtgcg aaaagcacaa ggagaaaaat ccaagctaga
    19081 gtcattatct gacaaagtgg caaaatggct gttttatgtc gcattaattg tgggtatcct
    19141 ggcattcatc gcctggttgt tcttgacaga tcttcctaat gcattagaac gtatggtaac
    19201 agtctttatc atcgcctgtc ctcatgctct aggtttagct atcccattag ttgttgctcg
    19261 ctccacatca atagcagcta aaaacggatt acttttgaaa aaccgcaatg cactggagca
    19321 agccaatgat ctagatgtga tcatgctgga caaaacagga acactaacag aaggaaaatt
    19381 tactgtaacg ggtgtcgaag tacttgatga tgcgttcaat aaaaatgaaa tccttcaata
    19441 tctaggagcc ttggaagcga atgccaatca tccattggct gttggcatta tgaattattt
    19501 gaaagaacat gaaatcaagc cttatcaagc tgaaaatcta aaaaatcttt caggagtcgg
    19561 gctagaggca acagtcaaaa aacaacaagt gaaaatagtt aatgaaaaag aagttgagcg
    19621 tctcggatta aacgtagaac aagcattatt gaaaccttac caagaacaag gaaataccat
    19681 cagcttcctg attttagaaa atcatctagc tgcaatcgtt gctttaggtg acgtagtaaa
    19741 aacagaagca aaagaattta tccgaacatt gaaagaaaga aaaatcactc ctgtcatgct
    19801 tactggtgac aataaaaacg ctgcccaagc agtagcagat tatttaggga tcgaagaata
    19861 ttatggcggt ttattaccag atgataaaga agctgttgtc cagaaatacc ttgaccaagg
    19921 gaaaaaagta atcatggtgg gtgatgggat caatgatgcg ccaagtttag cccgtgcttc
    19981 aatcgggatg gcaatcggtg cagggacaga tatcgcaatc gattctgcgg atgtcgtatt
    20041 gacaaacagc gatccaaaag atattttgca ttttcttgat ctagccaagc aaacgcgaaa
    20101 gaaaatgatc cagaacctct ggtggggggc aggttacaat attcttgcaa ttccgctagc
    20161 agccggaatt cttgcgccaa ttggtatcat attaagtcca gcagttggtg ctgtcttgat
    20221 gtcgttaagt acggtagttg tagctctaaa tgctttgaca ttgaaaatca aataataatg
    20281 aaaaaa
//
LOCUS       DACEUF010000046        20222 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.049, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000046 DACEUF010000000
VERSION     DACEUF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.049"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(50..1234)
                     /locus_tag="IXG20_001649"
     CDS             complement(50..1234)
                     /locus_tag="IXG20_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ1609850.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1259..2266)
                     /locus_tag="IXG20_001650"
     CDS             complement(1259..2266)
                     /locus_tag="IXG20_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1609851.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2438..2797)
                     /locus_tag="IXG20_001651"
     CDS             complement(2438..2797)
                     /locus_tag="IXG20_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609852.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(2769..3140)
                     /locus_tag="IXG20_001652"
     CDS             complement(2769..3140)
                     /locus_tag="IXG20_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ1609853.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3157..3459)
                     /locus_tag="IXG20_001653"
     CDS             complement(3157..3459)
                     /locus_tag="IXG20_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609854.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(3476..3946)
                     /locus_tag="IXG20_001654"
     CDS             complement(3476..3946)
                     /locus_tag="IXG20_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ1609855.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(3921..4199)
                     /locus_tag="IXG20_001655"
     CDS             complement(3921..4199)
                     /locus_tag="IXG20_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ1609856.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4196..5245)
                     /locus_tag="IXG20_001656"
     CDS             complement(4196..5245)
                     /locus_tag="IXG20_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ1609857.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5202..6131)
                     /gene="cpaF"
                     /locus_tag="IXG20_001657"
     CDS             complement(5202..6131)
                     /gene="cpaF"
                     /locus_tag="IXG20_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1609858.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6263..8017
                     /gene="ade"
                     /locus_tag="IXG20_001658"
     CDS             6263..8017
                     /gene="ade"
                     /locus_tag="IXG20_001658"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ1609859.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8115..8777)
                     /gene="trmB"
                     /locus_tag="IXG20_001659"
     CDS             complement(8115..8777)
                     /gene="trmB"
                     /locus_tag="IXG20_001659"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ1609860.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(8847..9626)
                     /locus_tag="IXG20_001660"
     CDS             complement(8847..9626)
                     /locus_tag="IXG20_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ1609861.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(9833..11044)
                     /locus_tag="IXG20_001661"
     CDS             complement(9833..11044)
                     /locus_tag="IXG20_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1609862.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11037..11774)
                     /locus_tag="IXG20_001662"
     CDS             complement(11037..11774)
                     /locus_tag="IXG20_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609863.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            11912..12346
                     /locus_tag="IXG20_001663"
     CDS             11912..12346
                     /locus_tag="IXG20_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ1609864.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12339..12683
                     /locus_tag="IXG20_001664"
     CDS             12339..12683
                     /locus_tag="IXG20_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1609865.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            12863..13873
                     /locus_tag="IXG20_001665"
     CDS             12863..13873
                     /locus_tag="IXG20_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1609866.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(13942..14886)
                     /locus_tag="IXG20_001666"
     CDS             complement(13942..14886)
                     /locus_tag="IXG20_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1609867.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(14870..17590)
                     /locus_tag="IXG20_001667"
     CDS             complement(14870..17590)
                     /locus_tag="IXG20_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1609868.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(17587..18807)
                     /locus_tag="IXG20_001668"
     CDS             complement(17587..18807)
                     /locus_tag="IXG20_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ1609869.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(19088..19933)
                     /locus_tag="IXG20_001669"
     CDS             complement(19088..19933)
                     /locus_tag="IXG20_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1609870.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         6174 a         4068 c         3324 g         6656 t
ORIGIN      
        1 aattccctct tgcatgcgtt tcatctttta tcctaacgaa tgctttggct tagccgcgca
       61 gacgctccac atcacgggca atcattagtt cctcatctgt tgggatcact aatactctta
      121 cttttgattc ttttgtagag atatctactt cttcaccacg caattcattc aacgctggat
      181 cgatttcaca accaaaccaa gacatacctt tgatgatttc actacgtaca tgtccgtcat
      241 tctcgccgat accagcagta aagacaattg catcaacacc attcatcgtt gtcacataac
      301 cgccgatgta ttttcggatg cggtccacga aaatgtcata agctaaacga atatgttctt
      361 tttcatagtt cttttcaaga tcacgcatat cactagacaa tcctgttagg cctagcagac
      421 cagatttctt gttcaaaaca ttgatcattt catcaatatc aatctctaat ttttccatta
      481 aatatgggag aacagctgga tcgatatcac ctgaacgtgt acccattgtc accccagcta
      541 atggtgtgaa tcccatagaa gtatccatgg atttacctcc atcgactgct gtgatcgata
      601 caccatttcc taagtggcaa gtgatgattt ttagttcttc gatcggtttg ttcaataatt
      661 ttgccgcacg ttccgacaca taacgatgac ttgtgccatg gaagccatat tttcttactt
      721 tgaaatcttc ataataagac accggtaagc tatataaata attttgcttt ggcattgttg
      781 agtggaaaga tgtatcgaat acagccacac tcaaaatgtc aggtaaaatc tttttaaacg
      841 ctttgatccc cattaagttt gcaggattat gcaaaggagc aaattctgct aattcagcaa
      901 tgcggtccat tactttctca tcgatcacta ctgaatcccc ataagtttct cctccgtgaa
      961 caacacggtg ccctactccg gtgatttcat cataagagct taaaatttgc agttcaatca
     1021 actgatctaa cagcattttt actgctactt cgtggttttc gatatccatg atttgttcga
     1081 atttttgatt atcgccgtac ttgatggtaa aaatagaatc ttgtaaaccg atacgttcga
     1141 cgatcccttt cgcaactacg gtttcttctg gcatttgata taattgccat ttcaaacttg
     1201 aactcccagc attgattgca attgttttag acatatgact ctccttctcg ttgaatgatt
     1261 acagagcaga tgatttccag tttttaaact cgttgaaaaa ttccgtcacc ttctctggtt
     1321 cttttaatga agcaagtttc acaagcaaga cttcttttac ttgttgtgct tgtggtcctt
     1381 ttttctgtaa gatcaaaata cttttttgcg attgtttatt acggaacaat tcatctggca
     1441 attgaatcat tccttgaaga taaccttctt ctttaaacca ttttttgatt tcttcacttt
     1501 gatccgtttc taaaaatcca ctcggcatca aaaacaaacc aaaaccatcc ggcttcacat
     1561 atttcattga ttgctctagc aataaatgat gtgcataact atggccttct tcggtacttg
     1621 ttaaaaactc ttttgctttt tcgtcattcg gataataacc gattggcaaa tcgctgattg
     1681 caaaatcgac aggatctatt aataattcct gcaacccgtc ttgatgaaag tattgaacat
     1741 ttgcttgtgt caaatcactt gtagaagctg cgacagccaa caatgtatca tcaatatcca
     1801 caccaattcc ttctgtttgg taccccgcat tcgataggtt taaaagaact gttaataaca
     1861 agttgcccat tcctgccgca atatccagaa tcttaaccgg tgctttttta tttgtgtata
     1921 gttgttcaat aagaaaaaca aataaaaaac cgatactatc aggagtgagt tgatggttcg
     1981 cctgcaaatg ttctgtctgg ctgcccttca ataaaagtaa ttgagacagt ctgcgccatt
     2041 cttctggctc gaaagaaagt ttcttcaact cttcataaag agctgtgatc cgttgcgttg
     2101 tctcttttgt cggcactcca tccaccacac gtacttgata atcatctatt aaattttctg
     2161 cattttcgac ataggcttct aaaaaggaag ttcccaatgc attctgtaaa agctgaatag
     2221 cttccaggtt ttgattaaac ccttgttcca ttttttctgg taacatggtg cacctcattt
     2281 ctagatatat tataacaatt ttttcacctg tttaattcta tcgaacaatt gacaaaattt
     2341 caatcgtttt cactgttttt tatttgtgaa acggcaaaca tttttttatt aaaaacagtg
     2401 ttataacaga aaagagggcg ttttcacgaa cattccatca ttctgtacta gtatctgtat
     2461 cattttttac ttcttctgtg aaccgaaaga taaacggtga gacttccacc tctaagaata
     2521 gggactgttg tacccactga tatgttacag atccagttgt gtagtaaaaa actccttccg
     2581 aactttcggc aagcgtagac tgttccgata aagccatctg cttcattatt tttgctatat
     2641 aataattttt gattcgtata ttaagatcca ttgacaaacg gtagctttct aatgctgcca
     2701 tcaataaaaa cgtacataaa agaaataaaa gcaagacaga aaataaaata gagcccttat
     2761 aacattttct actgatcgtt ttttgtccat atggcatatc catcttctcc attttcaaag
     2821 tgtacataaa aagaaattgt gtgttctttt tccaataatt gaagctcagt gatacttgtt
     2881 aacataggtt gatgtccgcc ggaagttctt cttttacgta tcattgcctg atatctttcg
     2941 atgatatact gtttatcctc gatcgtgtag tgcagttgct gatttttcac agagacattg
     3001 tgactgctct tgagttcttc ttctaattgt gctaaaaata ttagccactc tttttgatct
     3061 ttacgctgca aaaattcact tacttggatg gtttgctgaa tcaaaagatt gaatgtgagc
     3121 aatagactgc tcaatatcat cagtccaatt agacattcaa tcagcgtaaa tgctggaatt
     3181 acttgttttt tcaatactga tcgcttcttt ctccatattg acaaacactt tttcgattac
     3241 tccattatta tcgacaattt ggatatcgta tgggatcatt tcttcccttc gctctggtct
     3301 tttgtgatac atccgctgcg tcttgatatc ttcataaatc attcgaataa atagcagttc
     3361 ctccccactt ttcttttctg cctgaagcaa aaacagctgg ccaaagctca ctaagtagac
     3421 acagacagaa gcgatcataa gcgctgtaag ggactccaac aaaacaaatc ctgttttatt
     3481 caattctttt tgtatagcga ccactcccta tctggaattg atacgtaatc gtccgctctt
     3541 tttcttccca ataaaattga taagctttta gtgaactttc attcccggtt ctagcagcaa
     3601 aagtaataga agcatggcgt ttcaaggtaa tctcatcagg aatatctaat acttcccaat
     3661 ttattttatc tggattcgga acatgaaaaa tgatttgatt ctcttcacta ttccaataaa
     3721 agcctgtttg gatctgattc acaatagcga ttttttgcgt agaatagatt cgtttttcaa
     3781 attggccgaa aaattgatag actgccaatt cattcttcca tgcagaaaat gcgagaactg
     3841 gaaaagaaag gatcgttgta agtaataaaa tcagtaagag tgattcaaac aacgtatatc
     3901 ctgatgctaa aaccttacct tcaaggccgc tgatagattt ctgcatgttc tcgtttgata
     3961 tagccttcat tcaacaattc ctccacagta ggaactcttc cattattttg aagcgcatag
     4021 agctcttttt gtgtgtctac caactggatg atcgcttcac gactttcttg gtctacatga
     4081 ttcctataac gcgacaaatt agggacgaac aacaacacta atacactgat gatcagtaag
     4141 acgattagca tttcgatcaa tgtaaaaccg ctataataag atatttttct ctttttcatg
     4201 gcaaaacctc ctctatgctt ccgtaaacag gtaataaaac agcggcataa actaataaaa
     4261 tcaaagccgc tacgcccaaa aaaatgattg gctgaataaa atgcatccag tgatgaagac
     4321 gtttgaaaag ctgttttctt gtccattcgc tgtaaaaaat cagttcttta cctaaacttc
     4381 cctttgcctc tccctgtaaa acaattttac tgaattcttc tgatagaaaa cgataattct
     4441 ggaactgctc agccaatgac atacctgcct ccaatccttg aatcaactta gaggcaagat
     4501 gctgaatcag actttcttgt ttcgtttcct ttaagcagac tatgatttgc cttaattcga
     4561 aaccttcatg aaaaagcttt ccaatctcta gtgacaaata agccgattga tacaaggaat
     4621 atatcggtcc aatcaataat tgtctgctta tccattcact ctgatcaaca ggagacaact
     4681 tcatcagacg ataccttact agtaaagcca atagagtaac cgctaggatg aatagtagaa
     4741 cataccaatg aaagacttgt atcagctgaa taccccaatg ctcttgcatg accattcctg
     4801 aaccaataag ctgaggcaaa acgaacaacc gcatactaaa caagatacct agcaaaaagg
     4861 tgagcaatag catcggatat gcaagcagct ttttcaaatc attgcgaaat ccttcaacaa
     4921 gccgaaactg tttggcaatt tccaacaaag ttgtcggcag attcccatga cgttcagcta
     4981 actcgatctg taccagttgc tctgctccat atcctatttg cgaaagtacc tcactaaacg
     5041 cttgcccttc ctttagctga tgttggatat ttgccaaaat aacaggtgga aacgaacgga
     5101 tcgtgagtaa taaataaagg gattcttgca gactaaaacc agataaaagc agttctccca
     5161 tggtttgtgc aaactggttt tgctggcttc ttgttaatcc tttagaatag ttctgactgc
     5221 caaatatttt cctgagcttc ttcataactt tcctcccatg atttttttac tgattcttcc
     5281 tccataaatt gataactcca taaaacagaa ggatggtgat tggcatcttc caaaagttct
     5341 tgataaatga caccagctaa acattcagat agctcttctt ttttctttgt cagttcatgg
     5401 attctgacca atgtaccttc aagattgcgt gcatgtacgg ttgcaaaaac ccggtgccct
     5461 gttagtgctg ctcttaccgc agcatgtgca gttgattcat ctcttatctc gccaatgatt
     5521 agcaagtctg gacgatgccg taatgcgagt ctcaaaagtg tatcataagt ctgtgctatt
     5581 ttcggattga tctgtagctg cagaaaaaac ggttcttcta tctctacagg atcttcgata
     5641 gtaatcactt gcaatttttc ttccaaagcc aaccgataca ttagagaagt cttgcctgat
     5701 ccgactggtc cgctgaaaag gtataatcca cgtctgacgg ttttctgtaa aatagttgca
     5761 aaatctcgag aactaaagca acgatggggt gtttcaaaag taccgtataa tagccgaatc
     5821 actaaactct ctcgttgaag gtaatctcct acagaagaca accgcagacg aacttcctgt
     5881 ttttctaaag aataagtaaa tgcacctaac tgagcttttc ttttttctcc gatatccatc
     5941 tgtcccaaat acttaaatct tgagatcagt tgctgtccat ctgccgaaga gactttcttc
     6001 cagaatgttc gtttttttcc atagcgaaaa tagatgtaaa atacttctga agctggcaat
     6061 atatatacgt cttgggcatg tttggcaaaa ctttcactta tcatttgatc cgataatcgt
     6121 tttatttcca ttattctccc ctccacaaaa aacatacgtc tttttgatct ttttgtttca
     6181 aataaaatga atttacaaaa aaatattggc aaaaaccgaa tttatgttat acttataaaa
     6241 atttatcttg ggaggcactc cattggaaac aacattaagg aaatatatcg aagtagcaag
     6301 taaaaaaaag cctgctgacc tagtgatcaa gcatgctaag atcgtcaacg tctttacgaa
     6361 agaaatcatg gaaaaagatg tagcaatctg cgaaggaatg atcgtaggaa tcggtgaata
     6421 cgaaggaaaa agcgtacata atgctaatgg gcaatatcta gtgccaggat tcatcgatgg
     6481 ccatgtgcat atcgaaagct cacttttatc accaagcgaa ttttcaaaag tatccttgct
     6541 tcatggagtt actactgtag tgacagatcc ccatgaaata ggaaacattg ctgggagtac
     6601 aggtcttgag tttatgatag aagatgcaag acaaacactc atgaatatct tttttatgct
     6661 accttcttgt gtacctgtaa ccccttttga gaccaatggt gcaaatttaa atgcaaaaaa
     6721 attagcccct tttttacaaa aacccgaagt tttaggctta gctgaagtca tgaactatca
     6781 agcagtagca aataatgaaa cagacatcct agaaaagatc caattgatgc accagcataa
     6841 gaaaaaaatt gacgggcatg cagctggtat cggcatggaa gagttaaatg tttatcctgc
     6901 agcaggtatt cggacggatc atgaagctac aactgcaaaa gaagctaaag aacgtttgga
     6961 tcttggtatg tatttaatgg ttcgagaagg gaccgttgca aaagatctgg catcgctact
     7021 accggcgatc acacctgaaa attcaagaag atgcttcttt gtcaccgatg ataaactgat
     7081 caatgattta gtaaaagaag gatcaatcga tcatatcgtc cgtcaagcta ttgcacttgg
     7141 aatcgatcca ttacaagcca ttcagatggc aacattgaat gcagcagaat gttttgattt
     7201 gaaaacacta ggtgcgattg cccctgggta tcaagctgat ttcttcttaa cagacgatct
     7261 taccaagctg ccaattcatg aagttttcta taagggaaca ttagttgttc aatctgggca
     7321 actccaagac catttatttg ctgatcattc aaattcatat acgtgttgtc tgccaaaact
     7381 aaacgctcaa ccattaaatc cacattcgtt agagctgctt ctttcttctt cacaagccca
     7441 tgtgatcgaa attatcccga atagtctagt gactaaagat ttggtagaaa aagtggaccg
     7501 acaaggcgaa cgattcattc cttcaatcga aaaagaccaa ttaaagattg cggtgatcga
     7561 gcgtcaccat caaacgggaa acattggatt aggcatagtg aaaggatttc agctgaaaca
     7621 aggagcgata gcgacaacga ttgcccatga ctcgcataat ctagttgtcg ttgggacaaa
     7681 cgacgaggat atgctgtatg ctgctaatct tttgattcaa aaaggtggcg ggatgaccgt
     7741 tgtcaacaaa cagcaggaaa tagcttgtgt ttctcttcct ataggcggaa tcatgacaca
     7801 ggaacctttt tcgatcgtct atgaacagtt gaatgaatta ataactaaag cacatcaact
     7861 aggagcagct aaaacctttg atcctttttt gacactttct ttcttaacat tatctgtcat
     7921 acccgaattg aagatcaccg acaaaggatt attttcattt tctcgcttcc aattgattcc
     7981 accttgtgga aaaaccgatg aaataaatac attttaaatc aaataaatcc cagaaaatac
     8041 attacatctg taatgttcat tttctgggat ttatttatgg atattaatgt ttttatgaaa
     8101 gcagaaagtt agtttcacag acttttcttt ttggtcttat cttgaaattg agcttctact
     8161 cgataaatac gttgaccttt tgaagaaaat ttttcttcat actccgtcat aatattgcct
     8221 tcaaattgac tttgatgtaa atctaaccat acctgtttga ggatcatgcc atattgagaa
     8281 aaacttgcca gagaatactc aaacaatcct tgattatctg ttttgaaatg gatttctcca
     8341 tttggacgaa gaatctgttc atctacagca agaaaagtct tatatgtcaa tcgccgtttt
     8401 tcatgacgtt tttttggcca tggatcggaa aaattcagat agatttgatc tacttcgctg
     8461 tcagcgaaat actctgtcaa cgctgatcca tccacatgta gaagtttcag gttaggaagc
     8521 ggttgttcga tcaacttgtc tagtgcaatc gaaacaacac tgacctgcat ttctactcca
     8581 atataattga tttcaggatg ggcttttgcc attccagtga tgaactggcc tttccccata
     8641 ccaatctcaa tgtggattgg atgatcgttt tcaaacaatt cattccattt tcccttccac
     8701 aatgttggat cactaattac aaaattcggg tgagccgcta acatttccgc tgctcccgga
     8761 cgatttcgga cacgcattat ttatctccat tcctttcctc taattaaaaa ggtctggcca
     8821 aatctccgca gagtttgggc caaacctcag ccactgaaaa ttcgttttaa ttgtaaaatt
     8881 tcttcgttca tccgtcgatg atcttcacgt ttgcaatatc tggtgatttc ttggagcaaa
     8941 ttcatggccg cataccaatg aaatttttca agcatttcgt cagtcgggcg tatgccataa
     9001 ctgatcagcc actgattcca gctagacagt ggcacataat ttcctaaaat cgttgcaata
     9061 tccaccgcag ggtctgcaaa catgattgaa tcccaatcga ctagatataa ataattttta
     9121 catactaacc aattacgatg gtttacatct ccgtgtacta ctgataagtt ttccttatct
     9181 attgacggga tctgctcacg aagaaaacga tggacaattt gcagaaattg atttttctgg
     9241 agttcttccg gtaattcttt ttcatatttt tgcagcatct tctccggcgt catgacacga
     9301 ccaccgatac gggtcagcat ggttttaaga gaatttgaat gatgtaaatg gtacaaaaca
     9361 tcaatcacgt cgtttctttg accaatctca ttaggagtca gcaagcgacc ttccagccac
     9421 tcttgtgctg tcaaagtatc gccattcccc gttcgttttg tccaaacaag ttttggtgta
     9481 attccttccc ttgaaagagc agccaacatc ggagtcgtgt ttcgtttaat aaagactttg
     9541 tcctcatcac gcatgcctat gtatgttttt cctgtgtcgc ctttgatggg acgtaaccgc
     9601 cactcttggt ctaactgaaa ctccatcatg cattctcccg tcctcttact ctttgttttg
     9661 cagtacttat tattctagtc gcctgaacac tacacgtcaa gcacatatac gttctttttc
     9721 atttaattgc cattcaaaaa accagctttt ctcaaaaaaa atagaacgaa cagcattctt
     9781 actgtcaacg ctctattttt cattctcatt tttaccctac acagctttgt tttcagtctg
     9841 acatcttctt caaacgagtt ggtacatata gtttaacaaa gagcatagta acaatcaaat
     9901 aagccgctag cggaacccat cgctgctgcc catctaatac gactgcagca atcaaaccaa
     9961 aaataaccga cgcgataatt aatacccatg tgagtagata ctgaatcgcc tgcttcttct
    10021 gattcttatg gattggataa agctgaacga gaaccatata gcggaactgg ttcgctaaag
    10081 gtaacagttg gaaaccgatc aaatataaaa ataagaaacc gataatcaga ctaaaataaa
    10141 agtcgactac tccggcaatc aataacccac ctataattac taatcggaaa taaagaccac
    10201 taaaatcaga acctcttgcc aatcttcttg caaacaagta aagataagtg ttttttgatt
    10261 ctcttttaat atgagtcaat ataggatcca aatagcgtct tcttttgatc tttgccgtaa
    10321 tttcaggaac atctgtgaat aaattgataa attgatagat ctgatgcaac ctatgttctt
    10381 ctacatgcac cattttctcc caatccaatc ctttttcttg ttgcttccac aaaaaccagt
    10441 aatatagact tgcctgtact aatgcacctg ctattcctgc ccatattagc caataaaatg
    10501 atagaaacaa aaagatcaga gaagaaaaaa accagcctaa tttccaataa cgaataactt
    10561 gttgatctag tcgaaacaat tctgctctct tgatttgcag atgactgaat ttcaaactcc
    10621 ataatatgat cagatagacg aaaaacatcg aaaaggattg ctccgtacta accaccacta
    10681 atggcatggc caatccgcat gccaatatca aaaagatcaa cggaaaaatg caagaatagc
    10741 gaaaagctcg ttccaaatag gtttgcattt ctttttcttt aggaagtaaa aaaacaatat
    10801 ctgctgcctc tgcaaaagta gccatcttac ccacgaataa acagagcatc caaaatacag
    10861 agattatgag ccctccaatt gcaaacggtg gctgcagggt tttcaaccaa ttagaataat
    10921 ataatcccac accgccaatc aagaaaagac aaactaaaac aaaatgatcg ttcaatacat
    10981 aacgcatata tttcatcatt ttcttttgat gtcttgctaa acgtttttga aaaaaactac
    11041 tcatgtgcct gctcctttgt taaagcaata tagatttcgt ccaaagaaga atccggcagc
    11101 tgaaattctt ttctcaacat ctcaagagaa ccgtctgctc gtatctctcc gttatgtaag
    11161 accacaaaac gatcacaata tttttctgca gtagctaaaa tatgcgtaga catcaaaata
    11221 ccggctcctt cttttttcat ttctaccatc aattcaagca atgcattgat agctagtgga
    11281 tccaagccta aaaatggttc gtcgatgata tataaagaag gctcgatcaa aaaggcgcat
    11341 aagatcatca ctttctgttt cattcccttt gaaaaattag ctgggaacca gtcaagacga
    11401 ttagataacc gaaaagtctc caacaacttt tctgctcttt tcattgctat atccataggt
    11461 atatcgtagg ccatcgcagt gacttcgata tgttctctca atgtcaattc ttcatataaa
    11521 gaaggagtct ctggaatata cccaatttgt ttgcgatatt cttccgtatt tttctttaag
    11581 gatagatgat tgatttctat cgttcctttt gaagcttcca gcagaccgat aatgtgcttg
    11641 atggtcgtac ttttccctgc tccgttcagt ccaatcaacc ctactatctc tccaggctgt
    11701 acatcgaaac tgatatcttt taacacaggg agatgtccgt acccccctgt gacatgatcg
    11761 ataattaaac tcattctttt tcctccgcca tttattttta tttattatac cacgaagaaa
    11821 atgacggtat tttgtttttt tccttacaat agctttaact tatggtaaag ttaagctgaa
    11881 gtatttattc tttatagaaa ggatgtttta tatggaaaac tgtatttttt gcaaaatcat
    11941 caatcaagaa atcccaagct ataaaatata tgaagacgac aaggtctatg cttttttaga
    12001 catctctcaa gcaacgaaag gacacacatt agttgtgcct aaacgtcatg tagcagatat
    12061 ttttgaatat gatcccgaac ttgcaggaga ggttttttcg cgtgtgccta agattgctca
    12121 agcattggaa aaagcttttc cagaaatgaa aggtcttaat atcttgaata acaaccgtga
    12181 actagcctat caatctgttt ttcattcaca tatccattta gtccctcgct acagcaaaga
    12241 ggatgatttt tctattcact ttgtcaatca tcaagatagt tatggatcgg aagaactaaa
    12301 agctattcaa gaaacaatcg taaagcaggt gtcctgcgat gattaaaaac tttttcaaag
    12361 gactgttatt tggagctgct gctggcactg tcggaggtct gttattggcg cccaggagcg
    12421 ggaatgaaac aagaaagaaa ctgatcgaag aattggaaga agcaacaact ctgacaaatg
    12481 atttaaataa cagcctgaat cattttaaaa gcgcattgat cgaaacaaaa gaaacagctg
    12541 aatctgtgat tcctgctttc caagaagcaa tcaaaaaaga tatcgaagat tttaaattcc
    12601 aagccgaacc acgtatcaca caaatcaatg aacaagtaga aaaactaaaa gctgatctgc
    12661 cagagtatcc tgaacaaata taataatgat tattttctca acagatttaa ctatataagt
    12721 gagaaaagtt tgcgtatttc ctaggaatgt gatgaaattt tgaattttgt ttgaaactac
    12781 cacgacaacg tttttaaagg aaatgggata tggtatagtt aatatataaa aataataact
    12841 aattacagga gtgcaaattt taatgaaaaa aaaatcaatt attttggcag ctactagtgc
    12901 gttggctgtt ttaactctag cagcttgttc aggcgatact aataaagata tcgcaacgat
    12961 gaaaggcgga acgatcactg tatctgattt ctatgatgaa gcaaaacttg aatcatcgaa
    13021 ccaatcctta gtacaacgga tgatcattta taaagtattc aataacaaat atggcgataa
    13081 agtaaccgat aagcaagtgg atgcagaata cgacaaacaa gcgaaatcat taggtgatac
    13141 ttttgaatca caattagaag ctgctggata tacgaaagat acttataaag aatatattcg
    13201 taacaattta gcttttgaag ctggcttgaa agctcacgta gatattacag atgacgattt
    13261 gaaaactgct tggaaatctt tccatccaga agtagaagca caaatcatca aattatcaag
    13321 tgaagatgaa gcaaaagatg tgaaaaaatc agcggatgat ggagatgatt tctctaaatt
    13381 agccaaagac aaatcaactg atacagagac aaaagaagat ggcggtaaag ttaaattcga
    13441 ctcaacgaca acaactattc ctgcagaagt aaaagaagca gctttcaaat tgaaagacgg
    13501 tgagatctcc gatgttatta ctacaacaaa tcctacttct tatgcgacag aatactacgt
    13561 tgtcaaaatg gtgaaaaacc aaaataaagg caatgacatg gataaataca aagatcaact
    13621 aaaagatatc gctactgaaa ctaaactaag tgacaatgca ttcacaacta aagtaatcgg
    13681 tgaagaatta aaagatgcaa atgtaaaaat caaagatgat gcctttgaaa atgtgttgag
    13741 tgcattcaca acaacttcat caagtacgaa agactcaagt gaaactacag cttcaacaaa
    13801 gagttctgac acaaaatcaa ctgattcaac aaaagaatct tcaactgaag aaacaactga
    13861 ttcatcaaaa taaaagaaaa caaaaaaagc gaagacttat gtctcgcttt ttttgttttg
    13921 aatgaaaaaa ataaattttt gctataaatc ttttggtaca tagaaacttc ggttgtccat
    13981 accgaaaatc cgatcggtgt attttcctgg ttctgtttga cgaatcgaag tcaacatcat
    14041 ctgcatcgaa gcatcgatat tgtcgatttg atgtaatatt tctgcttcca ttattcttgg
    14101 acgtacaggt gagccatatt ctaataagcc atggtgtgct aaaaccatat gacgtaatac
    14161 gagaacatct tcgtcagctt cgctgatttt caacgcaaga caggctttag ttatttcttc
    14221 gtcaatcaat accagatgac cgatcaaatt tcctgcaagc gtatattctg tcgacatagg
    14281 accggaaagt tcaatcacct ttcctaaatc atgcaaaatg ataccagcat acaacaatgc
    14341 tcgattcagc tcaggatatt catcggcgat tgatttccct aagtgaagca ttgtcaaagt
    14401 atgataagct aatccactgg caaatgcatg atggtttctt tttgctgccg gaaactcaaa
    14461 aaattgtttt tgatatttat taagtagata gcgaacaata cgattccaat gcgcattggt
    14521 gatctcaaac aacaattgat tgatttcttc ttccatttct tctttcttca aaggggctct
    14581 ttccatgtat aaactaggct ctcctggctc atctaccctt gttaagcgca tatgaagaat
    14641 ctttacttga gggtttcctt gataaacctc tcttttaccg tttagaaaaa cgactgtgcc
    14701 ggctgtaaat ttattgattt cctcctccga agcatcccag tatttaccat cgatagtacc
    14761 tgaagtatct tggaaagtaa aagcaataaa tttttttccg tttttagcga ttcttacatc
    14821 tgcacttttg atcaggacaa attgttcaaa ctgctcatcg attactaaat cacgaatttt
    14881 tttcattatt tgctccttct aattgtttta cgatttggtt catctcatgg taatagctta
    14941 ccatttcttt atcagaagaa aaacaaatta tttgatactt ttctccaaat ttagctaaaa
    15001 gttcagctaa ttgatacttt cgctgatgat cataatgaag ccagccatca tcaatgatga
    15061 tcgggcatat cgaacggtta gcttctaatg agaggtaagc aaatctcatc gccataatca
    15121 gctgatcttt cgtacctgta gataactcat agatgggaaa ttctgtatat ccgtctgtta
    15181 aagtcagtgt atcatttttc agtaatatct tctgataatt tccttttgtt aaaataccta
    15241 aataatcacc tgctgctctt aacaactggg gtaattgtct ttcactcatc tctgttgaaa
    15301 gatcacctaa gaatgtcccg gcgatttcaa gtgctcccca ttcctccagt aattcctgta
    15361 attttgcttt ttgccggcta gcttgttgat aaagttcgtc caatgtgccg tctctttgca
    15421 actcttctac ttgaagttgg agtcgttgcc gattttcact tgcttctttt tcttcttgct
    15481 ggagttggga ttgcttttct ttcagttggc gcagtcttac agataatttc tcttttgtca
    15541 gctcttcagg aaatagtgga gtaagcattt ccgataattc attgtatctt ttcatctgct
    15601 gttcggcttg tttggctttt tgtaaaaaga caggtacttc cgaaggataa gataagcctg
    15661 cttgagtcaa ttgttcgctg tattcttgta tccatacttt ttgtttcttt ttcaattgat
    15721 tgatttcttg cttcaataaa gtattttgct gatagcttct ggcaaatttc atctgttcca
    15781 tctcttgtat aaaacgggaa agcaatacca ttttttctga aattgtctta tcttgtatcg
    15841 gtaaccattc tttaacaaac tcaaattttt ggtcgatttg tcgtagtttt tcttgtattt
    15901 cctgttctct tttatgtttc cgctcaaata cgccatataa tttctgataa tctatgactt
    15961 cctgtccata ttctttcatg agtttgagcg tatccatctt tcctaagtgc cgatgatccg
    16021 caaaacgata tacttcttgc tgaagaatct gtttttcttc tccagttctc ttttgttttt
    16081 cttctgcttc agctaattga gctaaggcta gatcaagttg tcctagtttt tcttgccaca
    16141 ttccttttat ctcagagaaa tcatttttag ttttaatttt tggttgcctt ttgtagaaga
    16201 aatagccaat catgcctgca ccaattagta aaaggatcac tttcaagggc attggtacaa
    16261 atatacctgc aatgattatt gctccgccag ccatcagcat atacggttta gatgatgaga
    16321 ctggtgctat ctctgactga ttttgtaata atgctggata ggtctcttcc aagtcattca
    16381 cttctgcttc aatcatctct tgctgttctt tagctatttg gagacgcaca gaaatctggc
    16441 tttctagatc agcaatcctt ctttcttcag ccagaagatg cttccattct tcttcatata
    16501 aaaattcttc cggtaacgga tttttctgcc aatgccacct ttgttccaaa atcttcaatt
    16561 ctgaatttgt ttctctagtc tcaatttgta atctttgcca ttcttcgttt aattgttccg
    16621 ctgtaaattg aagttgctgg agatctttta gttcctgctc ctgctctaaa taaaaataat
    16681 atctggctga ctgctgatcc atcccgctat gcttttccag tgttgcttct aattcttcca
    16741 aacgctcatt tgtttgctga taccgtttgg agaaagactc cagatctaaa agaagttgct
    16801 catcaggaag acctgtagtg ctgttttttt tgagagattg ccattcttca tataaagaaa
    16861 aattaagttc ttgctgtctg gctaatgaaa attgtttgtt cgcctctttt aattgtgcca
    16921 ataactgctg ctgttgcgac tcgtattcct gttcttgttg gatcaacttg ccaaaataag
    16981 cttcctgttc ttgtttttga tgaatttttt cttgcagctc tttccattca tttaattttt
    17041 gattgattag taatttttgg ccttttttct tgaataattg ttgtgctttc tgataatatt
    17101 cttgccgctt ttggaatact tgagaactgc ccgtgatgcc taaagaaatc agcgcatcat
    17161 gtagctcttt ttctctcaaa tgatctagtt ccatcaactg ctcttgctgg aaagtaaata
    17221 cctgctggaa taactctttc gtcaaaggtg cgatcagttt ttctagtaac tcatcacttc
    17281 ccacttgatc ccccagccac accttcgatt ttccgcgatt ttgctgcttg tagcgctcga
    17341 cagcaaattc accatataca ggatgtttaa gccatatttt gccgccatag gctgagccgt
    17401 cttttggtgt ataatccttt tttttacggc cttttgaagg gaaaccaaat aatattgctt
    17461 gaataaattg ataaatcgtt gattttcctg cttcgtttcc cccatagatc agctgattgc
    17521 ctgaataaaa gtcaaaactt ttttgacgcc attttccaaa agcagcaatc tcgatcctta
    17581 aaatcttcat tcctgatcct cctcaaataa aaaatcttga ttaagcagct gcttggcttt
    17641 ttcaagtgtt tcttcttgat attccggcag ttcgctcaat attctagccg cttcttgatg
    17701 actatatact tcatttaaaa tttgttggaa atcttgaggt gtctgatagt attgaaataa
    17761 ctgttccatc aatttagcag aagcagcaag tggggtcttc gtataaaaag tattctctat
    17821 gagagaaata cgccaaataa agaaatcatt tatcctatct gagaattcct ctaacaaata
    17881 gtctaatagc tctcctgatt gtatccgttc caaaacatct gtattgagat ggtcataatc
    17941 ttttaatttt atctcaatta aactaaatcc atcctctact tttgaaagct gctgacgaat
    18001 gtgaacaatg acctcttttg tcgttcttgc tttcctcaat gatatttcct ttgtcttcca
    18061 atagacctct gctactttaa tcggatgtat ttggcaactt cctttactta attcaactag
    18121 tagaatagat gtagaagctt gttccttttt agtatgacct tgaggcgcac ctggataaac
    18181 aattgtttgc ttttcattta agaccgttgg cacatggata tgccctaaag cccaataatc
    18241 gtatcccttt tcttgcatct ttgagggttg gaaaggagca tagtttcctt tttgagagac
    18301 gccaggttcc ccgtggtaca acccaatatg gtaatctgtg agttctctag aaggaaattc
    18361 catcactttg tctttttgaa tccacggttg acgataacta aatccactga gagagacagt
    18421 ttctccactt tttatcgtta ttttttttgt ctctaccgtt tcggatgtaa ataaatggac
    18481 atttttagga aaggagaacc aataccgctc tttttgatag aaatcatgat ttccgaatat
    18541 tataaataca ggtatatggt tcttttctaa ccgtttcatc tgttctgaaa aatgtttctg
    18601 tatttttaat gaaggacgat tttgatgaaa attatctcct gcaagtaaaa caaaatcaac
    18661 gttatttatt attgcctgtt caatgatatt tgataaaacg gtcagattcg tcttcaatag
    18721 tttttcctgt acagttttat caagagttgt cagtccttca aatgaccgat ccatatgtaa
    18781 atctgccgca tggataaatt tcaccattaa aaatttcctc ctttttttct tttatcatac
    18841 cattttcaat gttttttttc atcaataacc gaaaatttgt tcgtattatg caaagaaatt
    18901 acgataaatg gccgacttca aaaaataagt ttattcgaaa aaaagattgt gacatgattt
    18961 ttgtcacaat cctaaatatc tatgttatat tcgcttcgtt tgacactgaa gaagtactat
    19021 ttgtactgga atttatgctc catttttccg tatttagaat tctatgttct atactacctt
    19081 aattagttta tatggtaaga attctttcaa atgcttgaga tactccgtct ttatcatttg
    19141 aaacagtgac ataatcagct tttttcatga tttcttgatg actatttccc attgcaaaac
    19201 taatatctgc actttcaaac atagataagt catttaaact atctccgatc gcgattctca
    19261 aaggtttctt taaattgtag attgattcca aacgatcaaa caccacacct ttagatacat
    19321 tttgaggtac gatttcaata tttacaggtg ccgattcagt aacagacaca tttagcatct
    19381 ttttcaaatg gttaaatata ttagctttca attgacgatt aggaataaaa ataaaaaatt
    19441 tgtaaatgct gatagcatcc ttttctataa tttcctcaat attattataa gggattgaac
    19501 aaatagattt ttcatactca agctcatagt ttagctcttt gatagaaaat tcactcccta
    19561 cctcgtgaaa aatttttttc attttatttt tataattctg agattcataa cttccgagat
    19621 ttgtatatat tttggttgga aatttcgata ggacagaatc aatttttttt acatcatttt
    19681 tatcgaagga agactcaaat atcttttttt ctccatcaaa tccaacagaa ccatttaacc
    19741 ctactgctgg tatccttaaa ccagattttt tgagaatatt attaatgtct tctacttctc
    19801 ttccagaaca aatgaatgga aaacagtctt ccggtaaatg tttcattgtt cttgcatttt
    19861 tttgactaat gctctggtca gaattcaaaa gagtaccgtc taaatctata ccaattagtt
    19921 tcatttcatc catgtctcct atacgttgat tataaattca ctgcttattg taagtctaaa
    19981 tttctcacaa aacatatact aattatacct aatttaagta agtacaaaca tcattttaag
    20041 aataggaaag gacatttata tactattctc tgtatttttg atttataaat aaatatgaat
    20101 tgaatccaaa tcagctatat tactatttta taataaccaa ctataaaaaa cgaagttagg
    20161 tgaaaaacta acttcgatat tcataaaaga ctctattagt tcaacttagc aaaagattta
    20221 aa
//
LOCUS       DACEUF010000047        19060 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.050, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000047 DACEUF010000000
VERSION     DACEUF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.050"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(22..252)
                     /locus_tag="IXG20_001670"
     CDS             complement(22..252)
                     /locus_tag="IXG20_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609871.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQIDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(688..1254)
                     /locus_tag="IXG20_001671"
     CDS             complement(688..1254)
                     /locus_tag="IXG20_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1609872.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1254..2210)
                     /locus_tag="IXG20_001672"
     CDS             complement(1254..2210)
                     /locus_tag="IXG20_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ1609873.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2210..2866)
                     /locus_tag="IXG20_001673"
     CDS             complement(2210..2866)
                     /locus_tag="IXG20_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1609874.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3236..5254
                     /locus_tag="IXG20_001674"
     CDS             3236..5254
                     /locus_tag="IXG20_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1609875.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5681..8095
                     /locus_tag="IXG20_001675"
     CDS             5681..8095
                     /locus_tag="IXG20_001675"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ1609876.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8443..9996
                     /locus_tag="IXG20_001676"
     CDS             8443..9996
                     /locus_tag="IXG20_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1609877.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10377..12161)
                     /locus_tag="IXG20_001677"
     CDS             complement(10377..12161)
                     /locus_tag="IXG20_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609878.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12176..13897)
                     /locus_tag="IXG20_001678"
     CDS             complement(12176..13897)
                     /locus_tag="IXG20_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1609879.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13997..14758
                     /locus_tag="IXG20_001679"
                     /pseudo
     CDS             13997..14758
                     /locus_tag="IXG20_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            15725..15993
                     /locus_tag="IXG20_001680"
                     /pseudo
     CDS             15725..15993
                     /locus_tag="IXG20_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16665..>19060
                     /locus_tag="IXG20_001681"
     CDS             16665..>19060
                     /locus_tag="IXG20_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609880.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     "
BASE COUNT         6271 a         3375 c         3831 g         5583 t
ORIGIN      
        1 tctcattaaa aagcataacc attactccga tctgttttta aaattagttt ctacctctaa
       61 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
      121 ttgtttattt tcggaaaaag ctaatatagc taagattgaa acatcaatct ggaatttatc
      181 ttttttactg ctttgatcat aaaagtctgc atattcatca ctgatatttt ttctaataaa
      241 ttcttccatc ataaaaatca cctcaaaacg attatacgaa cttacgttct tgttgtaaag
      301 cgatatttga ggggcaaaaa aggggcataa tttgactgat attaagttag ctattgatat
      361 atgaatgaca tacaaaaata taaaatcttt taaaaatgct tatatatcaa tgtttttgcc
      421 attcttttat atatctgtta tatgtcaaaa aaatgtgttc aatttcaatt tgtatttgaa
      481 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
      541 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
      601 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
      661 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
      721 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
      781 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
      841 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
      901 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
      961 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
     1021 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
     1081 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
     1141 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
     1201 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
     1261 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
     1321 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
     1381 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
     1441 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
     1501 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat gaagcttgat tccttggata
     1561 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
     1621 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
     1681 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
     1741 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
     1801 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
     1861 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
     1921 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
     1981 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
     2041 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
     2101 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
     2161 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
     2221 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
     2281 acagccaaga aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca
     2341 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
     2401 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
     2461 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
     2521 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
     2581 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
     2641 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
     2701 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
     2761 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
     2821 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
     2881 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
     2941 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
     3001 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
     3061 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
     3121 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
     3181 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
     3241 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
     3301 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
     3361 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
     3421 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
     3481 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
     3541 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
     3601 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
     3661 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
     3721 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
     3781 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
     3841 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
     3901 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
     3961 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
     4021 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
     4081 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
     4141 ggaaaatggc tcttcgacga ataattccgg taacaatacc aactcttcag gaaatgctgg
     4201 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
     4261 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
     4321 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
     4381 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
     4441 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
     4501 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
     4561 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
     4621 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
     4681 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
     4741 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
     4801 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
     4861 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
     4921 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
     4981 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
     5041 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
     5101 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
     5161 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
     5221 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
     5281 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
     5341 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
     5401 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
     5461 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
     5521 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
     5581 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
     5641 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
     5701 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
     5761 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
     5821 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
     5881 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
     5941 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
     6001 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
     6061 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
     6121 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
     6181 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
     6241 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
     6301 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
     6361 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
     6421 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
     6481 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
     6541 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
     6601 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
     6661 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
     6721 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
     6781 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
     6841 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
     6901 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
     6961 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
     7021 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
     7081 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
     7141 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
     7201 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
     7261 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
     7321 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
     7381 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
     7441 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
     7501 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
     7561 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
     7621 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
     7681 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
     7741 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
     7801 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
     7861 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
     7921 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
     7981 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
     8041 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
     8101 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
     8161 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
     8221 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
     8281 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
     8341 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
     8401 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
     8461 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
     8521 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
     8581 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
     8641 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
     8701 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
     8761 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
     8821 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
     8881 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
     8941 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
     9001 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
     9061 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
     9121 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
     9181 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
     9241 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
     9301 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
     9361 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
     9421 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
     9481 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
     9541 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
     9601 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
     9661 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
     9721 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
     9781 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
     9841 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
     9901 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
     9961 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    10021 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    10081 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    10141 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    10201 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    10261 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    10321 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    10381 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    10441 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    10501 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    10561 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    10621 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    10681 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    10741 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    10801 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    10861 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    10921 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    10981 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    11041 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    11101 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    11161 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    11221 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    11281 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    11341 aaggcgagtt gattaatgaa acgaatggcc ggatagatag caaagttcat aaattgagca
    11401 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    11461 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    11521 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    11581 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    11641 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    11701 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    11761 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    11821 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    11881 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    11941 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    12001 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    12061 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    12121 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    12181 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    12241 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    12301 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    12361 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    12421 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    12481 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    12541 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    12601 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    12661 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    12721 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    12781 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    12841 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    12901 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    12961 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    13021 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    13081 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    13141 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    13201 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    13261 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    13321 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    13381 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    13441 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    13501 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    13561 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    13621 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    13681 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    13741 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    13801 gtacacctaa tgtaccggcg atttgtaaac agatcatcac aaaggtaagg agagcgagtc
    13861 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    13921 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    13981 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatagg aactatttga
    14041 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    14101 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    14161 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    14221 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    14281 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    14341 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    14401 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    14461 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    14521 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    14581 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    14641 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    14701 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    14761 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    14821 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    14881 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    14941 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    15001 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    15061 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    15121 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    15181 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    15241 tttgcttata aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag
    15301 aaaaatcaca aaggttattg ttatttttat atattctttt taagaatgat tgttttttta
    15361 tagaaatttt aaaaaataaa acacgttatt gatggatagt tagtttgtta tttggtaata
    15421 ttatagaagt tgactggtag tgatttattt ttcgtcgttt tataaaaatg ttattttttg
    15481 gaaaaattca caaatatgta acgaatgttt ttctggaatt tgctgtgtta aaaagcagta
    15541 tgactaatat aaatactatt gtcatttatg caatttttta tggatagtaa ttttagaggg
    15601 gaatcaatga aggaagaata gaagggaaaa atagttgcaa atacgccagt tagcaaaaat
    15661 agcaatacat cttcaagctt taaaattcga gagaatcatc ttggtagtgg aatcaactac
    15721 aacaaaagga ttaaaccaat taatagatag aattaccaat ggaaaaatag aaaaggtagt
    15781 tattctatac aaaagttgtt taattcgctt cggttatgaa cttagtgaaa ccctttgcaa
    15841 tatatggtac agaatataga aattattgac attacagaaa atgatgaaga tttaatacag
    15901 attgtcacta tttgtagata ccttttacaa ggaaaccgag ccaataaagc taagaagatg
    15961 attaaggagt tgatggaaga cgatacaagt taagaaagtg aaactacgcc caaataaaga
    16021 gcaaaaagag taattggacg tggaaaatgc aagaaatgaa ccatcgattc ggtggacagt
    16081 ttattagtaa tgacggtttg agaacatcca tgacgagaat gaaggaacat cctaaatgcg
    16141 tttagttgaa ccaagtgcca aataatatgg ccaaataagc gatcagttat cgatgaatcc
    16201 caagtattct aaactatata ttcattttga tgctaagcat tgatatattt ttttgggatt
    16261 gatgaaggtt aaccaataca gggactcact gatgaaattc tagagtctga ccttggtatc
    16321 aacgatttag tgattgtttc taatggaaaa gtataaaagt atataagtac attaacaaaa
    16381 taactattag ggttacgctc cagcttgtgc aaggcactgg caggaggaat cgtgaaatca
    16441 taattatggt taagccacac ggacaactaa aagtcataca aagctatcga atcaatcaac
    16501 agaagacaat cggcaatcat tagctttgtt tacagcttac attgcaacgc ttgagttgaa
    16561 ccgaaaatac gagggaggta acgcagccat tttgagttgg ggaatctttt tagaaaatta
    16621 tcgggtacct tgttgtttag tcaaaattat aagggggagt aatagtgagg aacaaatttc
    16681 ataaactgtt aagtatgttt ctagttatgc ttattgtgat caatcaattt gggaacataa
    16741 cggttctagc tgagtcttta gcagaacagc cagagcgttc gctgacgatc gaagttgatc
    16801 aagaggaggc tgctttgggt gagcagttcc agttgcgagt cagaggaaca ccagacctgc
    16861 taaatgcatt ggtcattgat aagagtgagg atttgattga aggggaagtg aagcaactca
    16921 atgattacga acgtctgatt acttattctt cccagcattc aggtaaatac gaattgaccg
    16981 caagaagcgg tgatattcaa tcaaatacgg tctcaataga aatcagtgta ccagaagaaa
    17041 gcacaagtga gtcttttaat gtaccagaag aaagcacaag tgaatctttt aatataccag
    17101 aagaaagctt aagtgaatct tttaatgaag ccagatcatc tgagccatct ataacgcagg
    17161 aatctcagat tgaaagacaa gaggagctct tgaattatga catcggcatc tctgctgagc
    17221 gccccacttt gccgagcagg catacacaaa cgttaaaggg atacattcaa ggaggcgctg
    17281 gcgatattct tgaagaatat ggggagatgg tcgtacgagt tcctagagca tcgattggct
    17341 taattgagtt cattagagtg gaggtcgctg atgattcacc tttaaagcca ctggcatttt
    17401 accaagaagg cacagacgtt gtttttccat ttacctttga ttatgaagga ttaaatgaag
    17461 ccgctcagtc gtatacattc agtatcacgt ttgaagttgc tttctttcaa tcaggcggta
    17521 ctgagccaaa taatgacttt caagtgagtc ttacttatgc tgagcgagca gcaaacacgg
    17581 aattttcaat tacgcctatt ccttcatcaa gacccatttt ttcgaaatta tactatggct
    17641 ctagagaaat cgacagccaa ggaaatcttg ggaatctgct gattgacggg caagacgtga
    17701 ccaacagtaa taaatttatt ttgcaggtaa attatgaagg atcacgaaaa gacggtgtag
    17761 tggttaccga tgtactaccg caaggaacaa ctttggcacc gaatccgact acatggagtt
    17821 ccactggaaa tagagaacct atttcaggtg gggttcggat cgcagaagta acgaatccag
    17881 gtgaagcaaa cgttagaact cattatgttt ccgaggaatt tgtggacaaa gtatctttta
    17941 ataaggaaac gaatacactt gttgtggact ttggtaatct agcagagggt caatcgtatt
    18001 ttattgaata tgctcttgaa gtgaccgatg tcgacttgtt caatgaagga agacaagagg
    18061 atgggttttc tcggaatcga gcgagtatga cctacaataa tcaggtaatg agccgagact
    18121 accgcttgag aatccctgaa agtaatcttc cggcaatctc tttaagaaaa agtgtggaca
    18181 aaaatgttat caatctcaat gaaaatgaac taacgtatac tctgcatgtc tcgatgtttt
    18241 ctggaacaag aaatacgctt gttattgaag atgttttgga agaaggcatg gaatttgtgt
    18301 catttctatc agatattccc tctttcattg atgccgaagt caataatgga atagttcgtt
    18361 tcagtgcgaa taatacgatt ggtggtccga caagttttac cctctctttt aaggtcaatg
    18421 tagaggcgta tgcggtcggc gatcaaattt taaactttgc aacgcttgtg aatagtgaca
    18481 atcgattgaa tacgagtact gtaagaacaa ggaaaatcga tgggcgtatt aaagtaatta
    18541 aaactgatga gaatggccaa aggctggcag gagcaaaatt tgaaatttta tcaagcgatt
    18601 cacaagtgat acaagaaggt ataacggcag aaaacggaga atttttaagt gaaccgcttc
    18661 ctgtaggtag ctatcaagtc cgagaaacag aagcaccgga agggtatctt ttagatgcga
    18721 cggtacatga tgtttttata aatgaatact ccatagaaga agtgacgtta accattgaaa
    18781 acatccttga tacgggtggt gttgaactaa ttaaacatgc tgaaggcagt ggtgaagtcc
    18841 ttcaaggagc tgtttttgag ttacagaacc gagaaggaga aacgctacaa accggtttaa
    18901 ctacaggaga agatggaaaa ctggcaattg acggtttagc accaggagca tatcagctgg
    18961 tagaaactca agcgccaata gggtatgaat tagatgcgac accaatcgag tttgagatcg
    19021 aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
//
LOCUS       DACEUF010000048        19047 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.051, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000048 DACEUF010000000
VERSION     DACEUF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.051"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            103..297
                     /locus_tag="IXG20_001682"
     CDS             103..297
                     /locus_tag="IXG20_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609881.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            566..865
                     /locus_tag="IXG20_001683"
     CDS             566..865
                     /locus_tag="IXG20_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609882.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(962..1396)
                     /locus_tag="IXG20_001684"
     CDS             complement(962..1396)
                     /locus_tag="IXG20_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1609883.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1628..2338)
                     /locus_tag="IXG20_001685"
     CDS             complement(1628..2338)
                     /locus_tag="IXG20_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1609884.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2328..3188)
                     /locus_tag="IXG20_001686"
     CDS             complement(2328..3188)
                     /locus_tag="IXG20_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ1609885.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3193..3831)
                     /locus_tag="IXG20_001687"
     CDS             complement(3193..3831)
                     /locus_tag="IXG20_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ1609886.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3846..4145)
                     /locus_tag="IXG20_001688"
     CDS             complement(3846..4145)
                     /locus_tag="IXG20_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1609887.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4188..5672)
                     /locus_tag="IXG20_001689"
     CDS             complement(4188..5672)
                     /locus_tag="IXG20_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1609888.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5692..6153)
                     /locus_tag="IXG20_001690"
     CDS             complement(5692..6153)
                     /locus_tag="IXG20_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1609889.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6171..7238)
                     /gene="ulaG"
                     /locus_tag="IXG20_001691"
     CDS             complement(6171..7238)
                     /gene="ulaG"
                     /locus_tag="IXG20_001691"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ1609890.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7456..8226
                     /locus_tag="IXG20_001692"
     CDS             7456..8226
                     /locus_tag="IXG20_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1609891.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8499..9326)
                     /locus_tag="IXG20_001693"
     CDS             complement(8499..9326)
                     /locus_tag="IXG20_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1609892.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9894..11129
                     /locus_tag="IXG20_001694"
     CDS             9894..11129
                     /locus_tag="IXG20_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1609893.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12128..12388)
                     /locus_tag="IXG20_001695"
     CDS             complement(12128..12388)
                     /locus_tag="IXG20_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1609894.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12415..13716)
                     /locus_tag="IXG20_001696"
     CDS             complement(12415..13716)
                     /locus_tag="IXG20_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1609895.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14008..14466)
                     /locus_tag="IXG20_001697"
     CDS             complement(14008..14466)
                     /locus_tag="IXG20_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1609896.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14479..15804)
                     /locus_tag="IXG20_001698"
     CDS             complement(14479..15804)
                     /locus_tag="IXG20_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ1609897.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15821..17233)
                     /locus_tag="IXG20_001699"
     CDS             complement(15821..17233)
                     /locus_tag="IXG20_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1609898.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17250..18014)
                     /locus_tag="IXG20_001700"
     CDS             complement(17250..18014)
                     /locus_tag="IXG20_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ1609899.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18118..18993)
                     /locus_tag="IXG20_001701"
     CDS             complement(18118..18993)
                     /locus_tag="IXG20_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1609900.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         6050 a         3925 c         2929 g         6143 t
ORIGIN      
        1 aaattgattc tttttattta tcataaagat atagatacat aatcattttc acttacatat
       61 atttgaatat taactaaaaa cagcaagaaa gaagaggatc aaatgtatta tgtagaagta
      121 aaaacaaaag gcgtcaaaaa caaacaatat gtgaaaggta tcagtaacga atatccttta
      181 cttggttcat ggaaagaagc tgctcctttt tccaaaccat gtgccataaa aataaagaac
      241 gagctagaaa aagaactgac ttgtggtaaa gctgttgtag atatcatcga aaagtaaatc
      301 caatgtatag aaaggaagat cacacatgaa aagcgacgta caaattgcta agattataga
      361 attagaagaa gaagacgcag tgttgacaat tgaaaaaaaa taaatgtttg tataccatat
      421 gattatttca atatgtatcc tggaattgga gatctcgtag agctatatca agatgaatca
      481 aatatacttg tcattcatca tgaaatatag agacttataa aattgtttat ttttataatg
      541 tatcagtata gaaaggaaat aaaacatgga ttatactgtt gaatttgaca gtgaactact
      601 taagcaattt ccacaagaag aacatttcat gtataaagaa gcaatttccc acaaagtgac
      661 agagtatttg gagcataatt tttttcgtat aaagccagtg cgtactcgtt cggcaaaagg
      721 aatatacgag atgaaagtca aaattaataa aaaaaattat cgcatagcat ttgctttaaa
      781 aaaaaatcat gtgttcattt tttatattag ttcgaatttg caaaaaattg tatttgataa
      841 agaggtgagt aaaaaaataa aatagctgag cattggagga tagattgagt gatactaagt
      901 ctatcctctt gtttttctaa agaactaagc tagaattttt tcttttgtac gttattatat
      961 gttatttgtc attaccaaaa agcacctttt ttcttctagt atctatgaca taagaaatac
     1021 cacccatttc ctttacttga acaaatccta ctgattcata aaattttttt gttttctcag
     1081 tatcatctgt caataattcg atttggactg gtccataact agtgatcttc tccaaagctt
     1141 cattcaaaag cactttgcct attccttggc gctgatacct cggatgaatg aggatatctt
     1201 gaataaatgc gatcgtcgca aaatcagtga tccaacgaac gattccgata atcttccctt
     1261 ccccattacg ctttatcaga gattcagatt gttcaaatgc tctagctaac cttgcagtgt
     1321 cttttgtgta attcgtccaa ccaacgctct tatataattc agaaatctca tcgatcataa
     1381 gggaatcaat attcattttt ttcttccttt cttatacaat tctccctcta ataatacagt
     1441 tctttgacta tttaatctat cacatataac tctcctgtaa agattctctc gattccttaa
     1501 aattcctctt tccttttaga atgcatactt tttgataaat aaaaaggttg tgataaattt
     1561 tcttcacaac ctctgtttct ctctatcaag tgttatcttt taattttcta cccttaccct
     1621 agcatctcta ttgcccgtag tatgcatact ttccatgttt gcgaaaataa tgtttatcta
     1681 gtaaatattg cgggatttca catatattgg ggttgatcag ctcgttttct ttggccatca
     1741 gacaaatttc atctaagatc aaactgtttt ctactgcttt cataggtgaa tccccccatg
     1801 tgaacggtcc atgtccatag acaagtactc cagggacttc gtttggatct aattttcttt
     1861 ccttaaacgt ctcgacaatc acattccctg tatgaacttc gtaagcttct cttacttctt
     1921 ctttcgttaa ttggcgtgta caaggaacct taccataaaa agcatcagca tgagtcgtgc
     1981 cataagctgg caaatcgcgt cctgcttgcg cccacatgac ggagtgcgtg gaatgagtat
     2041 gagtaatagc agttatatct tcaaaagttt gatacaaaac aacatgtgtg gggagatctg
     2101 aagatggttt taagctatct tcttctatta tatttcccga caaatctgtt accaccattt
     2161 gatctgcttg catgcattca tatctgacac cgcttggctt aatgacgatg actcctagct
     2221 cacggtccac ttcactcaca ttgccccaag tcaatttgac taatccgtat cgcggcaatg
     2281 ccagatttgc ttcaaggaca cgttgcttca ttgctgcaat tttttcttca caagacatca
     2341 tagccagcct ccttcaattt agaacttaag tagtcttttg cctgctggat ttctcttcga
     2401 aagtcttggc ttttttcact ccacatttca atcagaaatg ttccatcata atctagacgt
     2461 ttcaaatgtc ttaataatcc aagaaaatca acacaaccgc taccaaaagg aacatcacga
     2521 aactggcctg aatattcttt tgttacaggg taagtatctt ttaagtggat agcagtgata
     2581 taatcgatcc ctttctcaag ttctcgagcc ggattatttt ccggccatgc ggacaaattg
     2641 cctaggtctg gatatacttg tagaaaaggt gaatgaatct gttctttgat ttctaaaaat
     2701 tttgaaatgg agttcataaa tggatcatcc atgatttcca cggaaagaat gatgccatat
     2761 ttagaagctt cctttacgct ttcttttatt ccttcgataa agtcttctct ggaattcatt
     2821 gatttctctt cataataaac atcgtatcca gctatttgga ttactttgac gcttagatta
     2881 tgcgccaaat ggattgcttt tttcattagt tgtttcgctg cttttttctt ttctggatca
     2941 gcagaaccaa acggaaatcg ccgatgcgca cttaaacaaa tcgaatggat acgtacaccg
     3001 gagctgaaca tctcttctct cagctcagct atcttttctt cagaccaatc taatcgtgct
     3061 aaccgctcat ccgtctcatc aatcgacatc tcgataaaat cgaagtccat ttcttttaca
     3121 agggaaaaac gttctttcca tgaaatatct ttgggtaacg ctttttcata aatcccaatt
     3181 tgagccattt ccttaccccc agatacgaat gatctcatct ttgaagtttt gtgcggcctt
     3241 catcggatca tttgctgctg taataccacg accagtgata aaggtataca catcgatccc
     3301 ttcaaataag tgtaatgtgt gtggatttaa tccaccagtt acagaaacat taaagcctag
     3361 ttcaatcagt tttttgatct tcgataaatc tttttcaccc caagtttcac cagataacag
     3421 cgcatcacgg ctttgatggt agattacctg ccggataccg atgttatgcc aatccatggc
     3481 ttgttcaaaa gtccaattgc cgtagagttc tacttgcaat tcacctattt ctttttgtgc
     3541 tgcctccata gttggaatcg ttgcacaaca aatgactgtc atccagtctg ctcctgcttt
     3601 tgcaacatta ctcgccactg ttcctccagc gtctgcacat ttcgtatcag cgactagttt
     3661 tttatctggg aacatactgc gaatacagcg aatcgctttt tgtccttcct gtaagcaaag
     3721 aattgtgccc acttcaataa tatctactac atcaccaact ttcataacat ctcctaatgc
     3781 atgttctaag tcattgtgat ctaacgcaat ttgcaaattc ggcctgctca tcttagttcc
     3841 ttcctttact tgtttttttg gtaccacttt gtttcagcaa atttttcact gatttctttg
     3901 actgacatca catttttgat accgataact gtaactcctt ttttctctgc ctgagtaaac
     3961 atattcaaaa aattgactgg cgtaaaaact acatcgtatt ggccagctga actttttccc
     4021 tctgaaatgg cacaatgatg gattttggta attggtacgt ttagttcttt cattgctttt
     4081 tccacacttc tcatcatcat caagcttgtt cctgatccat ttgcacacga tactaaaatt
     4141 ctcatcttaa tccctccatt atgagtgttg tctaatcaga ttttctttta tactctctgc
     4201 ttttctgcat atgtgtcata gtcttctaca atcaaaaaat aaccatcagg atttttccga
     4261 tattgtaact gcggaatagc caataaacca atgacaacta cggcaattcc agcataacct
     4321 aggactttca tgattactgt aaacaatggc caagctgttg cccagtcgaa catcccgata
     4381 taaccaccgt atgttgacat cccaatccat gtagcgaata atgctgaacc gcctacttgt
     4441 atgattcctg aaagaaacgg gaacaggcaa gcggctttga atccacctcg gttattggca
     4501 taaactgcta taacggcatt atcaaagaac aacggaatga aaccggctat gacgatcgtt
     4561 ggtgatttca ataaaatcag taaaccaatc atcaaaaact gtcccaaagc accaaacaag
     4621 aacccaatcg ttaccgcatt tggcgaacca aagccaaaag tagcagctac gtcaattcct
     4681 ggaacagctc ctggtaaaat cgtgtttgag attccttgga aagattgagt tagttcatcg
     4741 acgaatgtcc gaacacctaa ttgcaagatc gccagatata ccgcaaaatt taaagaagtc
     4801 gtcataatgt agaataagaa actctgccct tcttgcatga attgcgcttg aatcaaataa
     4861 tctttgccta gtacaatcag aatgatccca aagaaaaata gcatgagtaa cgaagtagca
     4921 accatattct cattgaaaat cgacaagaat cctggtaatt tgatatcttc aatttttttg
     4981 tcttgcttga ttgatctatt tttcttttca tcccgctttt tcatccactc agctaatttt
     5041 gcaaatataa agattccgaa catttgctga tgtgccactg caaatccggc accttctgtc
     5101 aattcctgcg tgattcctac tgttaagtta gaagctactg cccaataaca acctaaaatc
     5161 agtcccataa aaatcaatac ttgtatccta cctagatcag gaaagcaaaa cagtaaaatc
     5221 caaaaagcgg tagctgcttg ttgaacctga acgtttccgg tagtgaaaac ggaacgcagt
     5281 ttcgtatatt tctgaaaacg gactaataag atatttaaaa caaatgcaat cagtaataaa
     5341 atcatcacat ctccaaaggt acggccaaat gtctccatta atccagcatc cacagcattt
     5401 tgtccaaaat aaggatctgt aaccatggca tccaaattga accgttcctt tagtccaacc
     5461 aagattggcc gaaaattatt gaccaaacca ccagagccaa cagtcaaaat aaagtaaccg
     5521 accgttgcct ttaagaatcc agcgatgcat tcatgaagcg ggcgtttcag caaaatatag
     5581 cctaataaca cgataaaacc aatcaggtat gctggctgcg tcaagatatt tgttgcaaaa
     5641 tacgtccaaa tgcttaacaa aaattccccc atcttttttc tcctctatca attaattata
     5701 ttctgccatc acaacttggt aatcatcgat ggagttcgta gagatgagac gctcaaccaa
     5761 tccatctgtc attaataatt cggataattt ttggatattt tctagatgtt cttctggatc
     5821 ttttgctgcg agtgtaaaaa acaaactagc cgttttctcc tcattctccc cttcactttc
     5881 aaaacggatt ggctgtttca acttggtaaa tgaaatagct gttccaaaaa caccatcact
     5941 tttttctgaa gaatgcggca ttgctacttg aggtgcgatc acaatataag gaccgtattt
     6001 ttctacacaa tggatgatct cctccacata cgtttcatca ataatttcct tatcgatcaa
     6061 tacaagacag ctttcggcga tagcttcacg ccaatccgtg atttctttat ctgggtaatg
     6121 aatcaattga tggtcaaaaa agtatttcag caagaaactc cccttctttc ttataaaaac
     6181 gacggaaacg gtgtatcatt ttcaatcgag aagacatctt caaatcctcg ataaaaatta
     6241 aactcaagat catctttgtt tgtcggatag atgaatttcc cgcctacttg ccagatataa
     6301 ggtttaaatc catacttcag gcgatccttt ttgaatttcc aaatttcagt gatctctttt
     6361 ggatcagcca taaaattaga ccaaatatcg tagtggacag gaatcactac ttttgcattc
     6421 aatgactccg ccatgcgcag catatcaaca gaagtaactt tgtctgttat ccctcgtggg
     6481 ttttcaccat aggcccctaa acagacatcg attttgtgtt cattaccatg tttggcaaat
     6541 aaattggagt aatgtgaatc tcctgcatga taaagattcc ctcctgatgt ttcgaataaa
     6601 tagtttactg caatttgatc catctcctga ggcatttttc cttttaatac ctcttctgga
     6661 tcttcacaag tgaccaacgc tgtccgatca aacgcttcta aagctactac attgatgtct
     6721 ttgattttga ctgcatcacc tggctttact gtaatcgttt tttcttcagg aattccccat
     6781 ttttgccacg tgcgaaccac ttcttttggc ccaatgaatg ttgcttcagg acaattttga
     6841 tgaactgctg cggctgtatg gatatctaga tgatcggaat ggatatgtgt aacgattaaa
     6901 gcgtctacat tttttatcgc aaaaggatcg attacgaaag gctgagtacg taaattaggc
     6961 tgcatatttt gaacgccact catacgcatc atctgatggc cttttttcat catgccattc
     7021 ccatgactac gttttcccgt cccacaccat agatcacata aaatattcgt tccttcatga
     7081 cttttcagcc aaattcccgt acagcccagc caccacatag caaaagttcc aggatccact
     7141 acttctgttt cgatttcttc attcaagtat gtgccccact caggaaatgt ttctactaac
     7201 cacttttctt ttgttacttc attgacagtt ttactcattt gcttgctccc cccatatcgt
     7261 tgttccaact tacaagtaaa tattaatgtg tatcagcatc catgaaaacc cttttatatg
     7321 accgtttgtt ttttatatga ttttctttca gtaatcaatg attgtttcag tataataaag
     7381 acagatatga atgtttaaat gaacaactag tcaaataaat ccatggatca agatgtccct
     7441 caggaggcaa aagtcatgaa aaactcactt gtcaatattg aaaaacgtca aaaagaaatt
     7501 ttggaactgc ttcagcagac tgaacaacta tcaactttcg aattagctga atcccttgat
     7561 gtatctatta gtacgatacg aagagattta aatttattag aagaaaaaaa cgagatcata
     7621 cgcaattacg gttattgttc ttataattac aaaaaccaga ctgattttga tcaatctggt
     7681 cctgaaagga ttaaacaagc gattgccaga gaagctagta aatacattaa cgattatgat
     7741 acgctgttta tcaattctag ctcgactgcg ttgaaaacac tggattttct tcaatctaac
     7801 catttgacag tcattacaaa caacgtgaaa atcaattact ctccgcacaa agataattgt
     7861 agttatattt taactggtgg agagatgcgc tttccaaaag aagcactagt gggcgacatc
     7921 gcattaaata cgatttcttc agtcaatgca gacatttgta tcattggctg tagcggagtc
     7981 gatgtagtac atggggtcac aacaaagata ttaaatgaat caacgatcaa cgaaatgatg
     8041 gtcaacaaga caatcaaatg taagatttta gtcgctgatc atcgaaaaat cggactgaca
     8101 tcaaagttca agattgctga tatttcagtc tttgattatt tgatcactga taaattttgt
     8161 tctgaaaaag tattagagga gatcagaaat acggggataa aaaccattca agtagattca
     8221 ttttaagaac caggacgtaa atatgtaaat aggattatca aaaaaatgaa taacaaattt
     8281 atcgataaat gaaaaacacg ctacgctcaa attgataaag gaatcccaag atgtatccat
     8341 ccaatcgata ccatcataat ctattgcttc ttttataacc gttatatgtt gcctaaatag
     8401 gaatcaaaca atatcgaaaa aaggaggtat gacaaaagtc acacctcctt ttttcgaata
     8461 aacggtattc aaatcagaac actgatttca caaccacttt atttcaggtc gttttcaatc
     8521 acctttaccg cgtaatcatt tccgttgaaa cgcgctattt tttttgcgtc catatatctt
     8581 tctaaataat catcttctcc taattgctta tggcaagaag caatttcata aagcaagtat
     8641 tccaatgcat aagtcgattt atgtttctta ttatactcaa tacccttctc acataattgg
     8701 attgctttat cataatcctc gattttacgg taataggtgg cgatatcata atatacaatg
     8761 cagaccatca gattatcaaa atcagtctca ctcaatattt taagtgtacg ctccacataa
     8821 actttcgcaa aatctaattc tccttttgac aagtagatca ttgccacgtt tgcagctgct
     8881 tgtaagtgat ggatatctgt ttcggtcgtt tgttccaaca cttgattaaa ataaaataat
     8941 gctgcatctg gttttttcgc ataaagatga tcaagcagtc cttgataaaa taaaatttga
     9001 tttaaaagat cagaattact tactttttcc tgatcgatcg tttgtaaaat tttataagat
     9061 tcttggtatt ttgatacaga attcaatgat tgaatctcat ttattttttt cctgattttg
     9121 tcttcttctg tttctactaa tatatcggag atctcgacat tcagcttttt gcaaagcgca
     9181 cttaagatat tgatgcctgg cgacatattc ttgttttcga tattgctgac agttgcttgt
     9241 tcacaaatgc cttctgctaa ttgtacttgt gttaacccct ttttctttcg gtattctttt
     9301 agtaatgctc catctacttt aatcaaaaca atttccttcc ctttaaaaaa ttatttctcc
     9361 tttatatcat acacaaaaaa aagttctaaa caaccaaaaa tgtttagaac agaataaaaa
     9421 ttttaatttt aaaataatat tcaaattaaa caactggcgt atctgctcct tgatttcttt
     9481 ttgtgttatc tgaataatta tcgtttacag ttataacaca tagtgaaaat tgtcatggcg
     9541 agaaagtcta agaatatttt taaacataac tatttctttc ttacgtgtgt ataatgacga
     9601 cagcacacct atcaattaat ataattattt tattttttta tgttctcatg tttattttta
     9661 atgacacaac ttctcgtatt tatctggcac aacatataaa tcgatcaaaa aattgttgcg
     9721 tgtttttttg aaaattggag tttcaacttg attacaaaat ttaaatttag aagttgtgaa
     9781 aacagcgaga gacagtttaa tattcaaaat atacataggc tactcgaacg ctgtgttttt
     9841 tagtatacta gtaagaaacg aagggataga ctcattccaa taaggaggat ttcatgaaga
     9901 caattctttt tgctcctgca gtattcaatt tagctgagac aacacgaatg attgaggttg
     9961 ccaaagctat gaaagaccgt tattactgtg tattctttgg cttttctaaa acttttgctc
    10021 ctttgatcac agaaagtggc tttgagtatc atttactagc gccgatcctt acgaaaaaac
    10081 aagaagaaca aatcataaaa tttgaccagt tgcgtacact taaaaaccct tttaccaaag
    10141 aaatggttgc ctctcgtatc actagtgaac agagtctgat tgctaagttt gagcctcgac
    10201 tgattgtgac aggttcgaat gtgacaattt ttttatcggc ccgcaataaa aagattcctt
    10261 tagtatatgt taaaccctat gctttgagtg aacctttttt tcaatcagag catgcgctga
    10321 tgccccacga gttacgtttc ttcggccctt taagcaatcc cctttggcac tggacaaaaa
    10381 agcgtatagt aaatacccga tggatcccca atgccttcaa acacgctgca caaaaagaaa
    10441 tgcttccttt accaaaaaca agtttacaaa tgatggatgc tgatctaaat ctcatcacaa
    10501 cgcctaaact atttttggaa gaagaacagc tgccggaaaa ttatcgaatc gttggtccaa
    10561 tttttgctaa gctcagtact tccttgcctg aagaagtgct aacttttata cagcgaaagc
    10621 aacaagaaag aaaacgtatt atctatttcg caatgggtag ttcaggcaat ccgcgtatgt
    10681 tacgcaaaat tttgttcttc ttacaaaaac aaccggatct agcggttatt gcacctattg
    10741 actatctgct tgatgacatt caatctgact cagaagatct tttaataaca gaatatctgt
    10801 ctagtcatct tttgcatgaa ttcattgatt tttcttttat ccacggcggg gaagggaccg
    10861 tccaaactgc ctgtgaatcc ggcaaaccgt ttatcggtgt ggggatgcaa tttgagcaaa
    10921 gctgtaatat tgactattgt gtaaaatacg gaaacgcaat cgccctcaca aaaccagtga
    10981 ctgaaagaaa actgcagatt gctctgcgcg aagtttcttc acccaagatt cgtcagcagg
    11041 cacgcacgct gcaagccgtt tttccaataa atggtccaca acaagcaatt caagaaatcg
    11101 aaaccttcct gagcaaccac ccaatatagt ttcaagtaga aaaagctttt ttaatagaga
    11161 ctcatcagta atttcgctat tcgagaccca gacagtgaaa atacaaaaaa agtaaatagt
    11221 ggattgaaaa gttagagtta cttaaattac ggtgtctaaa aaacacaaaa tcatccttaa
    11281 aataaaagat ctgacgctat ccaaatgagc ataggtagcg tctgccattt attcttagtc
    11341 aaattctttg acacctttct ctggttggat catctcaacg tttcttcaaa agaaagacca
    11401 taataattag gcaaatacat tttcttaaaa tgtgtcatat agatctagaa gcatcgcttt
    11461 gtagagattt gtgccgctag tcccctcaac aattactaaa tcaaatgtag gatttaccac
    11521 tttatctttg ataatcaacc tccctcttct gttgagacaa tgatctcgca atccgatgca
    11581 tactcctata taaactgcca gcaatcccca catattgagt agatcatgtc agaatgttct
    11641 atgtcgtcag caacgataca ctaaaagtca cgttcaccat gactgattgc tattcccagc
    11701 acaattattt ctatacacgt ttcatgattt gaataaacac tcacatcata catattacca
    11761 ttcatacacc atactgcagc ccctactata tggttgtaag ctcccaaatc tatatttgac
    11821 ttgatcactt tggtagtaga tctactaatt ctaaatccat tgctgataaa atttccattt
    11881 tttcctcctt ctttctgcca aaacagttaa aacaataata atctttatgt aaaaaagggc
    11941 tgtgacaaaa gccttgtcac agtcccatat tccgaataaa cggtggtcaa aagcttgcat
    12001 ctgcggtaat aagttcaacc aaacgaaaaa taagccaaca tttcacaaaa atttaagaag
    12061 caattttcgt aaatttcttc ttatttcttg aagctaacca cttctgttac aaccactttt
    12121 agaacattta aagcttcgat gtgacaaacc atctatgctg ctcgatcata aaattgatag
    12181 caaaaattcc acaaagtaca tatgtccccc atgaaaacaa aacattcata tgcggtacaa
    12241 tgatctttgg aagaatcacg atcaaaaatg atgccagtgc ccaaatccat cgattgattt
    12301 gaatatgata tgtagctagc atattcaata taaggtgact agccgttaat aaaaagataa
    12361 aaacaaaaaa taaaatcagc caatacattg tttcccacct tctaaaaacc aaacttagac
    12421 agtctggact tcgttttctc cacgactttg ttcatctgac agcatctttt tatcataagc
    12481 ttttacaaat ggataataga taataaatgc tatcaatgtg ttgatcatga tcaagataac
    12541 aacacgccag tcatttcctg ctgaaagata accgccgatt ggagctggta atgtccatgt
    12601 tggcaaaaca gtaaatctat tgaccatttc taatttgact gcaaaataac tgataatagt
    12661 caaaactaaa ggaactaagt tgaacgggat gaacatgtaa ggattcaaca tcataggagc
    12721 tccaaaaatt atgggctcat tgatattgaa gataccagga ataatagaaa aacgaccaac
    12781 ctctttcaag aattttgatt tacagaagaa tataaacaaa acagatacca cgatcgttgc
    12841 tcctgcacca ccaattgtta ccgtccattg ataaaactgt tcaggagcga tatagggtaa
    12901 ttgggtggca gctgtaccat ctgccaatgc ctttgcattc tcatccaaca tgatcagcca
    12961 cattggacga acaacagccc ctacaatact catgccatgg ataccaaatg accacaacaa
    13021 atggatcaaa aacattggta gtaaaacacc aagcaaatta ttccctaaaa tattggttaa
    13081 aggctcgaat aacgataata aggcagcgtt tacatcaaag cccgcgacgc ttcgaatcag
    13141 ccaagtaatt gttaaaatga ctgcacctgg aatcaatgcc tcaaaactac gggcaacagc
    13201 aggtggcact tgttctggca ttttgatagt aatatttcga tttttaaaga aacgatatac
    13261 ttccactgca aaaatcatag ataagatccc cgcaaacatc ccagatgctc ccaaagaact
    13321 cataggtaag acccagccaa gaggtgcctc ttcaggagtt aaaacgttta caggaacttg
    13381 taacataaag aacgttccta aagataatat accacctgtc acactatcta atccatatga
    13441 tttagctaga tccgaaccca taacaaaagt tgcataaata gccatcaatc ccatcgtcat
    13501 cctaaagggt aaaacaatcg ttgccgaata aggggcaata aattcattcc atccaggaaa
    13561 cggtaaattg ataaagacca taaaaaatgt cccaatcaaa atcaatgaca atgttgaaac
    13621 aaccccatta cgaattgcta ataaatgacg ctgtgtacca atttttgcca ttggtgggac
    13681 aagtttctct tcgacccatt tcatcagctt ttccactaaa accactcctt ttcaataaaa
    13741 tatgaaccat tcattgcatg acaacgcaaa gaatgttcat aactaattat ataattaaga
    13801 aattttattt caatatattc tcttaaataa tcaaatttat ttttaagaat cctgaaaata
    13861 agttttctaa atcataggag caacaaaaaa attcgataat taacaaaaat caaaatgatt
    13921 aaatctagtg attgaaacat atcatttcaa aaaaattttg taaattcacc cataaatatc
    13981 accctattta acttttttca tcgtacacta aaattcaaaa caactatctt taatctttac
    14041 ttcttggttt tgttttaata aatttccttc aaaaatgcct ggagaaacta caatgatcac
    14101 cgtatctttt tgtgcttgct ccaacttctt caaatctata atagctaaca gtgtcccttc
    14161 ttttacctcc tggttacttt caacccgtat ctgaaacgga cttccttcga gttcaactgt
    14221 atctaaaccc atatggatca gtatttttct gccagattca ctttcgatcg ttatggcatg
    14281 tcgggtagga aatatattat cgattattcc tttaactggc gcatggatat atccatcatg
    14341 attcgttata gcgtatccaa ctcctatcat ttttgaagaa aagacctcgt cttttactgc
    14401 tgttaatgga ataatttgac cactagctac agcttttaga atgtttcttg ttttaaagaa
    14461 atgcactttt tatcacctct actttaatgc tggccaatat tttttgttta ctttgatcat
    14521 ttcatctagt attttttttg cgactctggc atttggtacg gtttgattca atgtaaatgc
    14581 ttgtaaagct ttttgataag aaccttcaaa gtaagcatct actagtaact tctctactgc
    14641 aacttgtgcc tccatcaatc ctttatgaaa atcactgatt ggttttctta gtgtaattgg
    14701 ctccacacca gttgcaccaa catatgccgg tatttcaaca actgcatctg accgtagatt
    14761 cggaatcgcc ccttgattag gaacgattag cataaagcga cgacgatcat cattcaacaa
    14821 ggaaatagct atatcgacga tatattgtcc atgctcccca aaattaaagt tcaatacttc
    14881 ttccacaata ccgcggcgta ttttatcagc catttctttg gtatttttct ctcgtccatc
    14941 catgaccata ttagcacgtg tatactcttt gtcagtatgc tcaacgacca tatcaggata
    15001 taagtaatac tctaagtaat tattcgggat attacttgga aaatttttcg tgatgactga
    15061 catcatttga aaggtttttt gccaagtttt atctcctata ttaaaatcgg cgacttgcat
    15121 ttcttttgtt attagttttt caataatctc tggcataatt tctcgttgca attctttatc
    15181 ataaatcgat gtataccagc caaagtggtt taaaccataa tacgtaacga tccaattctt
    15241 acgatcataa ccgtaattca ctgcaatcgt ttcttcaatt gaaatagtca tatcacaggc
    15301 attaatcatt tttacattcg gatatttacg acgaacagct tcagaaacga tcgactctgg
    15361 atttgtataa ttcaaaatcc atgctttggg ggcatagtct tgtatatgat cgacaatttc
    15421 caataaacct ttgattgagc gcatcccata agcaaatcct ccgagtccac acgtttcttg
    15481 tcctactaat ccatgtttaa gtggaatctt ttcgtccttt tctcgcatct ccaatcctcc
    15541 aacacgaatt tgtgagaaaa caaaatcgca tcctgtaaaa gcaagctttg gatcatccgt
    15601 tgcacgaatg cgaatatttg aaaaaccttt tcttttcagc ataaatttaa caatcaagta
    15661 catatccata tttctttctt catcaatatc atataaacgt atttcagata atggcaaccg
    15721 tttttcattg ttcaaaatgg cttgaacgat tcctggtgta tacgtactcc cgcctccagc
    15781 tatagtgata atctgttttt tcatcatcat cttttccttt ctattcttct aataatttat
    15841 tgaattctgc cataacataa gtcacttcta agccgatgat aatttgataa tttttttcag
    15901 ttggtttaat gatcccgctt atccctaatt gcttgattcg ctgttcctcg accatggtgg
    15961 gatccactac ctctacccgt aggcgtgtat tacaactata gacatccagg atattggctt
    16021 ttcctccaaa cgcttgtagc atttttgttg ccatatattc ataattttta cttgctaacc
    16081 ctaactctgt ttcttctaag gttacttcat tgccaaactc tgccttagct tcacgtccta
    16141 atgtctgaat gtttctctta ttgatcaaat aataaaaagt aacgtaatac aaggcaaaaa
    16201 agaaaattcc gattggaaga atcaaaatag cattggtagc gattcgaaaa ttgatgagat
    16261 aatcaacaat acatgctccg aatgtaaaac caattcgtat attgaataag taacagacaa
    16321 tccctgctaa accggcaaaa agtgcatgga tcacgtacaa cataggagaa agaaacatga
    16381 aactaaattc aacaggctca gtgatatttg caataaatga agttcctgca ccgcttccca
    16441 tgattccttt gaccatctct ttattttctt tataagctgc tttatagata gctagacata
    16501 tagctggtat accaaacatc attactacaa aaaaacctga taaaaacagt ccagcctgag
    16561 gatctccgtt gataaagcgt gtcatctctc ctctgaaaac ttccttgcta ggagatgtat
    16621 agcttcctaa atcgtaataa atatacgtat tcaacacatg gtgcagtcca aaggggatca
    16681 gcaaacgatt caaaaacata aatatcccca cacctaatgc tcccatatca acgagcagtt
    16741 ttgcaaaatt atcaatccca atttggatga gcggccagat aaagcccaaa aaaacagctg
    16801 cgattgtttg tagaatcatc accatagtca atgggaattt ttcgcccgca aaataagaaa
    16861 aaactactgg taatttttgt tctttaaagc gattatacgt ccatgccgct actaaaccgg
    16921 aaataattcc accaaaaatc cccatattca cttctgggtc cataatttct aagccttttt
    16981 tcaaggtagc aatcgctaaa aaaccagtaa gtgcaggtat tcctttgtcc ttatttttga
    17041 caaaaccgat tgcaactcct atagcaaata agatatccaa gtttccaaaa acggcatttc
    17101 cagctgcacc aattactggg atgttcagca tatcgtcagc tgataaacgc atgatcaatc
    17161 ccgctaatgg catcgctgca actggaagta gcatcgctct tccaagcttc tgcacatttg
    17221 atttaacatt catttttatt cctcctctat taaatatcct catatgttac taactcaatc
    17281 aaaggatcat ttttcacttt ttgagcaaat gctgaatgaa ttaaaaattc gacttggtgg
    17341 gtacgaggat acacaaaact tgaaccattt aacaattcct gatccaaata cgctgcgtga
    17401 cacatgactt cgatcgtttt atcttctctc aatatcatga gtaaacttgc caaatattct
    17461 tccacactcg tattgatttg tcgttttttt tcatcagttt ctcctacgac atcgaaacaa
    17521 ggctcaaaat aatcgacatg acgaacttca tcttttcgtt caaagttgct ccgaaccggc
    17581 aaatcatatt tttttgctaa tgtaacaact acctcctgat tttcatccaa agtatgagta
    17641 tgatgatgac tgtctaaatg agttggtatg atccccgcat gataaatttt ttgaatctga
    17701 gcattccatt cctgatacag ttgatctttg tcaatgttaa atggatgctg ataataagat
    17761 aaggatctga actctccgct ttcgataaga gtatcgacag ttttgaggag tggtttgcca
    17821 cacgtcaatg ttaaatggac tcctacacct agcctaggca tttcctttct tagtttcaca
    17881 gcatgctcga aacccggcat atttgccatc aatgtagttg atgtcaaaat tcctcgctga
    17941 taagcatcca taatcccata attcacacca tggctatagc caaaatcatc cgaattaaaa
    18001 atcacttttc ccatttcatt ctccttcttt tttctactaa aataggcgca accaaataat
    18061 catagtccat ttactatgtg tcatttggtt gcgcctatag atagttacgc ccttatttta
    18121 atagattctt gctagatgaa ttgccaaata gatgatttca ttttcttgaa tggcaagttg
    18181 aaattgctgt tcgatgatat ctttaatctt tagtgctgta caatacgctt tgctatgttt
    18241 aaatgtttca gtaaaaaaac caatattttc tactggcgtt tcttcctgat aaatcaagcg
    18301 tttaaccagg aatttgatat gaaccattaa gcgatgataa gtcagtgatt ctttatcata
    18361 aacgtttgaa taggattttt ctagatggga caaaatcgtg gtcgtcattt ctactatttt
    18421 taagctcttt aaactattag gctctcctag ctcagcatta atgatatgca tagtgatgaa
    18481 tcctacttca tttgacccta actcggtttg atatcgatga ttgattcgct cgaccacttc
    18541 tactgctata tcatattcat ttggataaag gaatctcatt tctggcaaca taattgctgt
    18601 gacatctcta tcttctcttt ttcgttgaat agcaaaataa atatgatctg tcaatgtaat
    18661 atagatattt gaatctaatg gagaggagat cctagctgtt atcagttcaa tcacttcttc
    18721 cgcaaaatcc ataaattcga ttggaatctg cgagactatc tcactgaatc gtttgatctt
    18781 ttctttagaa tctaatacaa ataatttgtc agcatcctca acatcaaatg gcttactcac
    18841 tttagcagaa aagccaatcc ctttccccat cgcaattttt tctattccat cgtcatcgat
    18901 caatacagca ttatgattca gctttttcat cacatacacc gttaattcac ctcctctata
    18961 tgataaaaca aaacaacttg cagactcctt catacatatt tttgtctcca aacaattttt
    19021 aaagacaaaa aacgacctaa gaaagaa
//
LOCUS       DACEUF010000049        18984 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.052, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000049 DACEUF010000000
VERSION     DACEUF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.052"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(330..647)
                     /locus_tag="IXG20_001702"
     CDS             complement(330..647)
                     /locus_tag="IXG20_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1609901.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(648..902)
                     /locus_tag="IXG20_001703"
     CDS             complement(648..902)
                     /locus_tag="IXG20_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1609902.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1162..1380)
                     /locus_tag="IXG20_001704"
                     /pseudo
     CDS             complement(1162..1380)
                     /locus_tag="IXG20_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3193..4233
                     /locus_tag="IXG20_001705"
     CDS             3193..4233
                     /locus_tag="IXG20_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ1609903.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            5057..5389
                     /locus_tag="IXG20_001706"
     CDS             5057..5389
                     /locus_tag="IXG20_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ1609904.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            6008..6349
                     /locus_tag="IXG20_001707"
     CDS             6008..6349
                     /locus_tag="IXG20_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ1609905.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     NEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            6368..6658
                     /locus_tag="IXG20_001708"
     CDS             6368..6658
                     /locus_tag="IXG20_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609906.1"
                     /translation="MKFFEVKSPYYYALIAAKNEEECAELYEQVVTDIENREEFIRCL
                     HELNRNEAIQEDAKKISEETLQPIGMEEATKDIFTIIDEQKSCVLSIDSTLV"
     gene            6850..7083
                     /locus_tag="IXG20_001709"
     CDS             6850..7083
                     /locus_tag="IXG20_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609907.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARGYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWREHNGIKDLKPARPVIRRKKLKK"
     gene            7503..8174
                     /locus_tag="IXG20_001710"
     CDS             7503..8174
                     /locus_tag="IXG20_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1609908.1"
                     /translation="MKKWLFGFLGVALIVFCSVFGYVSYQKHEGDVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTCKFIVSEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            8227..9855
                     /locus_tag="IXG20_001711"
     CDS             8227..9855
                     /locus_tag="IXG20_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1609909.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAEKANIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYSNNPFNQEEHHEKTKADAVTYGAKFLKA
                     DGGLFGTGIKATPLEGAEFAAKNAEGKYYSFCGR"
     gene            10364..10765
                     /gene="tnpA"
                     /locus_tag="IXG20_001712"
     CDS             10364..10765
                     /gene="tnpA"
                     /locus_tag="IXG20_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1609910.1"
                     /translation="MDNEYRYSKTTVSLVNYHFVFCPRYRRKIFVNSEVDARFKALVQ
                     EIAKENEWNIIAIETDKDHCHLLINVQPTYSPSKIMKIIKGKTSSIIRQEFEHLSKMP
                     SVWTRSYFVSTAGNVSSEIIKRYVENQRTRY"
     gene            10789..11907
                     /gene="tnpB"
                     /locus_tag="IXG20_001713"
     CDS             10789..11907
                     /gene="tnpB"
                     /locus_tag="IXG20_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1609911.1"
                     /translation="MKKAHKIRIYPNCRQEIQFQKTFGCVRFYWNFLLDQRITNYKKK
                     KENADYKEDKTTYASLKKMEEYAWLKEVEAQPLSQVAMDLNKAFKNTFNSTFGFPKFK
                     SKKYSKKSYRTAMGIKVNRNYFYVSKVGWVKMAETLRFNGKLMNVTISQSKTEKYFAT
                     FLVDIENFQKDPVTESIGLDLGLTHFCITSAGEKIGNERFYRSLEKRLVVEQRKLSRR
                     LEVAKKHNRKLEECKNYQKQRVKVARIHEKIFNQRNDFLHKLSSRITDENQIICIEDL
                     NVTGMVRNHKLAKSIADVSWSEFVRQLTYKCEWKGRTLVKIDRFYPSSQICSSCGHND
                     GKKELDIREWTCSNCGTNHDRDINASINIRTEGLSGIA"
     gene            11973..12338
                     /locus_tag="IXG20_001714"
                     /pseudo
     CDS             11973..12338
                     /locus_tag="IXG20_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            12351..13106
                     /locus_tag="IXG20_001715"
     CDS             12351..13106
                     /locus_tag="IXG20_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1609912.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQKQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSEKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFAFLLFLFIKKIRRRSE"
     gene            13103..13930
                     /locus_tag="IXG20_001716"
     CDS             13103..13930
                     /locus_tag="IXG20_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609913.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVAHQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETKTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIIL
                     LTPISDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            13940..14188
                     /locus_tag="IXG20_001717"
     CDS             13940..14188
                     /locus_tag="IXG20_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609914.1"
                     /translation="MKIIKMLAFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINMLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            14219..16330
                     /locus_tag="IXG20_001718"
     CDS             14219..16330
                     /locus_tag="IXG20_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1609915.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IRENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYSGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LDNAANYGRDYILQLHMKAKNDAKGIAPNVATVTFNNDPNKGIQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEARLTYKEGSARVIDMSTGKDITSEGTLTYDSNLRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLQSEKNIDNQAVAAVENVSNKTNVVTIGVDPNLPQVIVPRTGSTHLVTI
                     LVVSLVLLVLVTLSYVALKFN"
     gene            complement(16366..18417)
                     /locus_tag="IXG20_001719"
     CDS             complement(16366..18417)
                     /locus_tag="IXG20_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ1609916.1"
                     /translation="MKLLNPKTVLAIGTFAATLGFAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNANKVLNDSKAT
                     QKQVDSAKNNLDSAYKGLKKKPAPTVNKAELQALYNRVKSTAKGDFTEESWNDFQTAL
                     SNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPYMVTINHIDNQTGQVIATDQ
                     AQVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSQTITGNTTITFNYNQVHQINL
                     LCNNNYKNEGRVDILKEQIINVTHGESVTLTAPAIQGYVLDDRVEPTNSVTLNNVTES
                     QNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRNDD
                     PSISVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELVWLNNYRQQNGAAPM
                     QFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVDEI
                     LSDTGAVNAMIMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANPIF
                     K"
     gene            complement(18537..18746)
                     /locus_tag="IXG20_001720"
     CDS             complement(18537..18746)
                     /locus_tag="IXG20_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609917.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAIKLNKLAEETIDTNASTK"
BASE COUNT         6714 a         2917 c         3545 g         5808 t
ORIGIN      
        1 aaagttacgt caattttgat taaaaatata ctacttgaac ctctcatgag taaagtcacg
       61 aaatttttgg gaatagagcg tacttgatat cttatttttt cactaatagc agtgtctctt
      121 attcaccaag cgatccccgt agttcctacg gttcttaatt tttctctaga ctaatgattg
      181 tatctttaga ccttcggcca taacatggag caaaataaaa aggtactaag tcttatatat
      241 caatcttcga gtaaccgacg gttaacaacc atatcgagcg gcattaatca cccacctgta
      301 gaggatgagc gacttttgcc taattattgt taaaagccta gtttcatttg tttagattgg
      361 aaaatacgtc ctacaagtat ctcttttttt tcatggtcga tccgataaaa taaggcatag
      421 cttttgccga tcacgatccg atatgttggg atatccattc catagatttc ggaaacttcc
      481 tcatgcatag ttggaaaatt tttggttagt tctaaagcct tctggatcgc acctacaaat
      541 ctttttatgc tggctggaga cagagcaagc tcattttccc attcttctat tatcgtttca
      601 aggctggtct ggaaactttt agaataaact agcgtgtact ctttcatcta ttcactccag
      661 ccgtattttc cgaaagcttt taccatttca atttctgaaa ctgttaaacc tttctcaaag
      721 tcttggaaac tttcttcaat caatttgcga tcgtgttctt ttgctatttg agtagtatct
      781 aatatgaata agttgccttc ttgtttggct tggatagtgt ctccgctttt taatccaaga
      841 gagcttggaa ggatcactcc cacgctattg ccgatctttc taacctttag ttcttttccc
      901 atgttatcac actcctttta gtttttgata aataaattat aacatgttat aacaacaatt
      961 accaacggtg tttcttaagg gattccactc catattatta ttggacaaaa taagctactt
     1021 agaacgctga aaacatatac aaacgatatc aaaatacgag tgaactactc accatacact
     1081 tccgttaaca tccatttaag taggtatttt tattatatat agatttaaca tatgtcggtc
     1141 ggttgattga tatagctaaa gttataactc aaccgaagcc gatttatcta cacgaataaa
     1201 gttgcgagtg ttctcggcta attcataaaa attgttatgt gttccatata gcaatactgg
     1261 aataacttta ttttcttgaa tctcagaagc aactctatac acggtgaccg catacttaaa
     1321 tccattagac caattacctg ataaacctcc agttttttgc tcttacttaa atggatcaat
     1381 agaaatttca tcacaaacct atagaggatg agtgacttct gcctaattac gttaaaaaat
     1441 attgaaagta actctatgta gcaaaaaata tctagaattt catttcctca aaactgatcg
     1501 atattgtatc caaaaatata tactacctaa aataggtagt atatattaaa atatgtgctt
     1561 ttgttagaaa aatcaataca taaattatca tatctgtata tgtcatttag gtattttagt
     1621 tatgttataa tagttttgct gtctgctacc ccggcctaat ctggcaaggg gaggaggaga
     1681 tataatgaaa tacctttttg attttgtttt tgcaatcaag ccatttgcag aggtaactgc
     1741 acactacatc tgcaagtggc ttgattgcaa aattgccaaa aaagacagta agcctacccg
     1801 ttaattctat aatatagaaa cgtaaaaaaa aaccccatca gcctgcacgc tgatggggtt
     1861 tggtgatata atgaaatacc tctttgttac tcataattat accataatta gtcacgaatg
     1921 caaccaatag aaaaaactac ttagaacggt agatcatcgc taataacaat gcattgtcca
     1981 ccaaatggat tttgtgaagc attaaataca ttgttattaa ttatcgctgc ttttaactag
     2041 gcctctcccc ttctattata aaagaagata tatatcattt cttaattacc tactacctta
     2101 aaccaaaatt taagtactac ctaatttaag tagtacttaa ataatctaaa aaatagttat
     2161 cccaattgtt atgattttat aatacgatta tttgtaacaa aatgaatatt atataattaa
     2221 acactaccat atttaggtag tgtttaatta ttagcaaaac aaaaaatatt tattctattc
     2281 tcttaacttc aactaagtta tgctgcacat ctttatataa atcatatgca actcattagc
     2341 tagacaaact acaagcatgt agttttatct gttaattatg tactacctaa ataaagtagt
     2401 ttataattaa caagtatata tatttaaaaa tttaaaatta atttttttat gacttgtaca
     2461 aatttatctg atattctctt attaagaact acctaatttg ggtagttctt attttttaca
     2521 ttttaagtag tatttaattg caatttggta gtattttttt attatcttat tcctagaacg
     2581 gtagtactta atataaatat aggtagtatc taatttaagt attaatggta gtatctaatt
     2641 taacaggtag tatgtaacta ctttaaagta atttttttca gattaacaca gtagtatctt
     2701 attaaataag ttaattctaa aggtagtaca taattattat atatatctaa cattggtagt
     2761 acttattgta ttttttagta gtatataatc tattgttttc tagcctaaag ccttgtctta
     2821 taagaataat ataatatata ctactgaaat aaggtagtat atattactag tagtggtagt
     2881 atgttttata tgcataaggt agttcttaat ttattttttg ttttgatact ggtagtactt
     2941 aattatcctt aattttaaaa ccaaatttta ttcatacgtt cttctggtag tatttaatta
     3001 taaataatat ttatttaaag tagttcttaa tgactttaaa ggtagtacat aattcatttt
     3061 atttcttatt ttaggtagta tttaattaaa aaacagtttt aatgcgtttg cattttttat
     3121 ttatctttgc tattttcgaa aagagtaggt aattttacat aaataaaaca acgtgaaagt
     3181 agaggatcta caatgactga ttttaatttt tttaaggcgg atagagttta caacgaatta
     3241 ttttatcaat ttccaaaagt ctttattgtt tctgacgaat acaaaaaaat gaaagattca
     3301 actaagattg cctatatgct tttaaaagca agattagaga tcgcaatcag caaacggcaa
     3361 atcgatgaag aaggtaatgt ttattttact tatacgacaa atgaactatg tagcgtatta
     3421 aactgccaaa aacaaaaagc gatagcaatc aaaaaagagt tggaatcctt tggtttatta
     3481 ttacaaaagc agatgggatt taacaaacag ttagggaaaa ataatcctaa tagactatat
     3541 ctagcagaat taaaagtctc agaaaatgat atctacttac tcgaaaaatt tgatagagag
     3601 aatagggaaa atgttgataa atcagagggt atgaaaatca tacccaccct cgacgaaaaa
     3661 tcagacgctg aatcccttgg ggctcaagag ggtatgaaaa tcataccgtg ccaaaacgtt
     3721 gataaatcag agggtatgaa aatcatacca gaacttaata ataatatatt agacactaat
     3781 agacacaata tagacactga aaaagaccgc ctacaagatc aattgttgtt agacaatttt
     3841 gagacaatta tgacaaacga cagcattgct acgtttgtcc ctgaacgagt attaaatttg
     3901 ataaaaacat tttcttcaag ttacagtgaa gctcaaaaaa ccgtccagac tattcataat
     3961 gcaaagaaaa aagctgaaat agaaagtggt atttcgatag tttttgaaga actcgatagt
     4021 tattatgtca atgcagaaca agaattatac acgacactgt taaaagccta tcaaaaatta
     4081 aaaaccgaaa aagtcgaaaa tatccagaac ctgatttttg tctatgtaaa aaattggttt
     4141 atcgaaaaac caatagctgc taaagtatca agtgaaaaac gtttgaatta tgaaagctcc
     4201 ccaagcacta ttacgaaaga ctggttagag tgaaacttta ctgacaaagt gattgttgct
     4261 tcatggagta acattaaaat taaaaatttt ttattattct aattatatta cgaatattac
     4321 aaaataatag ctattttaat agaatttata agttaaaata ttatgataaa aatcacaaac
     4381 gtagcctttt acgtcataag tgttaaaaga aataattaat ttcaaatata gaaaagggaa
     4441 cgtatgtttg catactcctt ttttttgagt tataataata tgcgaagaga gataatttca
     4501 ttatttaaac aagctaaaaa tcttgatatt acttcttttc aggcaatacg aatattattc
     4561 gcttacattt tttgtataaa tttaattgtc tgctatgctt gtgatgaatt gattttcgtt
     4621 ctgcactttt ggatgataaa tttggacaat tgaaaataca aaaaaaagaa aggcggtatg
     4681 aactcggaag gctggtaacc ttcgtcagcg ttcatacacg gcattaagat ttggccctta
     4741 atgcgttaac ctttcagtgt tacggtatta cccgcaatgg atttagtata acagatattc
     4801 aaaagtgata ccactttttt tggctgtgtt aaggttaaaa atacgtgata ttacgatgaa
     4861 tctatgccta agcataatgt tataaaaatg tccgtgtggc tctttgccgt atgacgaacg
     4921 ctgaaattag agggaacgca tgtttggatg cgttccctct tttttgtttg tcatgaacga
     4981 aaatcaattc tctatttttg aaaaataaca agaaaaactt gttaaaaata tttaaagaaa
     5041 ttgggtggtt tattttatgt catatacagg aattttgagt ctcaaagata tttgtcacta
     5101 cggtaaacgg tgtaccgcta cagagaaaat cacgaagaaa ctatcaactg gccaaagtaa
     5161 aaccgttgtg cagtgtaaga aatatataat ccagaaagac aaagtctcag aggaaatgat
     5221 ttattacatt gggaaacaaa agcaaataat tttaaaagat cctatcccct taaaagagct
     5281 atatccaaca atcaaacatg tttatgacca aaacggtgta ctgattggtc gaagaaaaaa
     5341 tggagtcttg cgttgtaccg caaaagggat ggggcgttta atcagctgaa ttgatcattc
     5401 tcataacttg tattttatcg tttttcgctt gcttgaaaat attgtcgaga cttctaaaag
     5461 aacttagaat cattagaaaa ataaggagta gacttgatct atcaacaatt cccttcgggg
     5521 agtgacgggg taggttttaa cgattggtaa cgtaaaaaat tggtcaatcg agtaaatgcg
     5581 cttgtttgga tagtggtgaa atgaatttgt gtaaataata agcgcagagt aaacaagagg
     5641 gaatttagaa gttagacaag ttagaatagc gtctagatta atcatgagca gctactaagg
     5701 gatctaggct ttgacgcaaa ctcatggaat aaggagtagg tcttctcgta atggggagga
     5761 cgcacaagaa attgtgtcgt gtgctgcagt acatgtagat acttgcgagg acgttggtca
     5821 ttggatcaat cgagcattta ccgctgtcta ctagggtttc tcgtttcgga cgccataaac
     5881 gagtcgatat tttttatgta tttttaagta aacgaaaaac gataaaatat gaacgagtcc
     5941 tgaataaagt gaaaaaattg atataaaaca tagagaaaat gaaatttacg taatggagga
     6001 atgacaaatg aaaaattatg atccgaatat acgattgggc acacatacta ttaaggtttc
     6061 tttccaacgt tgggattaca aaggttttgt gacgtttaga agaggcggaa attgtaaagg
     6121 attagatgtt ttagcattaa acgaggatga tttatacgat caaacattga ctgataatcc
     6181 aattggattc ggcttgctac ctgaagatga tgaaggtaat gaatggttta aaatgacgct
     6241 tatgaatgat aatggagacg aattatctgt tgaagatact tggtcatatt tgagtgatta
     6301 tattgtcagc gttgaaatca ttgaatttgt agcggataaa gaagaataaa aactggagga
     6361 ataaattatg aaatttttcg aagtaaaatc accatactat tatgcgttaa tcgcagcaaa
     6421 gaatgaagaa gaatgtgcag aattgtatga acaagtagta acagatatcg aaaatcgtga
     6481 agaatttatt cgatgtttgc atgagttaaa tcgaaatgaa gcaattcaag aggatgcgaa
     6541 aaaaattagt gaagaaacgt tacaaccaat cggtatggag gaagctacaa aagacatttt
     6601 tacaattatt gatgaacaaa aatcttgtgt attaagcatt gattctacat tggtttaata
     6661 gatccaagat tctataattt ttttcaaagt aaaaagaaat taggtaatga ttctcagacc
     6721 aattacttag gaaaataacc atacaaatta gattttacag ataaaggatt agaaggaatc
     6781 acggcgatta ataaaaaggt agtattacct taggcattga gtaaggaatt tatttaggag
     6841 gaaaattaga tggcgcgacc agttttagat gcgatggata tggatacttt gacaaaagga
     6901 aggtatgtac agctgttagc tcgtggttat tcacctaaag atgtattcaa agccctatct
     6961 aaaggaacag atatggaaaa agaaagatta cgaaaagaat tcgactattg gagagagcat
     7021 aacggaataa aagatttaaa accagcacgt ccagttatta gacgaaaaaa attgaagaag
     7081 taagaccaaa gcgtatggcg tacataggct tttttatttt gataaaaata atgaaaggaa
     7141 ggaaaactca tgaattgtaa agaaatcgaa aatagaaaaa aagtgtcgaa agaaatggaa
     7201 gaaaagttac taaaaacaat gaaacaaaaa caatgaaaca aaaacaatga aacaaaaaca
     7261 ccttaaaaga ttatctgtcg cgcaatatat caatgatatg cagttaacag gaaaagagaa
     7321 agcttgtttg ttaggttcaa tgaaaaactt tgaacagttg agacgaactt atgtgaaaaa
     7381 aagttctaat tgtcaactat tgttagaagt ctcatgacat tcattaatag aattcatttt
     7441 taaaactttt tctatggaaa tgagaaaaag tttttttgtt ttgtctgaaa ggaggcagat
     7501 ggatgaaaaa atggttattt ggttttttag gagtcgcttt aatcgtcttt tgcagtgtgt
     7561 ttggctatgt tagctaccaa aaacatgaag gagatgtgtt taagcaaaat atagaaaaaa
     7621 agatgcccgt agatcaaatc aatgcacacg caaagagtta taaggaggat gcaacgaacg
     7681 tcaataatga tatgtctttg gggcaaatgt tgtccataca aaaagaagca atcgaaatgg
     7741 gcgtcaataa acaagtattt gcacaaattc agattcctgc acttggttta gcattgccga
     7801 tttttaaagg ggcaaatcaa tacactttga gtcttggagc ggctacttat ttttatgagg
     7861 atgccgaaat gggcaaagga aactatgttc tagcaggaca caacatggaa atgccaggtg
     7921 ttttattttc agatattcaa aagttgtcat taggggaggt gatggactta gttagtaacg
     7981 atggcgtcta tcgttacaag gtaacgtgta aatttatcgt atctgagtac tttaaattaa
     8041 ttgatggcgt tcctgaagag aactctttct tgtctttacc aaagaaagga gagaaaccct
     8101 tattaacgtt gtttacttgt gtttatacgt cacaaggaaa agaaagatac gtggtacagg
     8161 gagagctaca ataacaaggt tgtaatcaac gcgagtcgtt gaaatatgaa atgaggagag
     8221 taaaaaatgg caataaatcg gtatgtaaaa aaagctggaa tgcttgttgg aatgttagga
     8281 atcattgcag gctgctttca gggatatcga gcatatgcag aagatgtcac gcaaaaaacg
     8341 ccaccagaga aggtcaatat cacggttcat aaattaatgt acgatcaagg aacgcaattg
     8401 aatgttgata ttgacgggat caaaaatgat ggttatacgc acgatcaata tcctacaggt
     8461 gtaacgaaat acaacaaggc agattatggt gatgttgagt ttacgttagg aaatattacg
     8521 gatcaagtat taccgaggga agattctgat ctaaccaacg cgaaagtgga cgaaatcgtt
     8581 aaagatgtag aagacaaggg atctaacagt gagtacgtta agaatgcaac caataaaatt
     8641 acttcagctg tagatgaaaa tggtgagatt acttttgctg atcaaccagc ctatgtcaat
     8701 tccaaaggga atgtatatgt ggtttatgaa agtaaatccg ctgctggatt agtcacacaa
     8761 aaagcaaaac caatggttgt gattgcgcca atgactgata atacaggttc aggtttttta
     8821 aaagacattc acttatatcc taaaaatatt gtttctaagt tgagttttga attaacgaaa
     8881 tttggtgacg atggcacagc tcaaagcaaa caaacacctt taaaaggggc aaaatttgag
     8941 ttatacaagg gcgaacctgg aaaaggaaca aaattaggag atcttgtatc ggatgatcaa
     9001 ggaaagctga ctgctactga tttgacgtta ggaaaatact attttgtgga agttccttca
     9061 gaagttgtag tgggatcaga taaagaacca acagcagatc aatatttatt aggcgcagac
     9121 gctcgcaacg atgcgcacaa taaattaacc tttgaaatta cgaatgatgg cgtgacaagt
     9181 gacttaaaag cttcatacgt aaactataaa gcaccagtga tcgataaaac agtaacaaat
     9241 ggaacaggac aagaacattc tttccaaatt ggtgacgcag taaattatca aggaacgatt
     9301 catatcccaa cagacattgc aggcggagca gacggtatca cagtaaacgg agtgaaaagc
     9361 gaaacttctc cttacagtgt atttaaatgg ggggatacag caggacaagg actttcttat
     9421 gtcgctgaaa aagcaaatat caaagtcaca aataatgatg gttcagtcgt attaaaagaa
     9481 aatactgact ataaaatcga aaattctgat agtggttttg tgatcgattt tatcgtaaat
     9541 aatggacaag taagtgatac agttgcgaat ttacatggtc aagatctaaa aatgacttat
     9601 aacatgtttg tgaatgatag tgctgaaatc gcaaatccat taacaaatag cgtggatttc
     9661 gtctatagca acaatccatt caatcaggaa gaacaccatg aaaaaacgaa agccgacgct
     9721 gtaacttatg gcgcaaaatt cttaaaagct gatggcggac tatttggaac aggaatcaaa
     9781 gcaacaccgc ttgaaggcgc agaatttgct gctaaaaacg cagaaggaaa atattatagc
     9841 ttttgcgggc gataaaaccc acggttttaa ccgtgtggat gatagcccgc actgtactgt
     9901 aagagcatag ggaaacttgt gcttagtgga atatataaaa tctcgtatat cccaacaaag
     9961 aaactgaatt gcggggaacc cctaaagcga gtaaaacgac aacgtagtct tgaaataggc
    10021 aaacgtgacc gtggcgaaag cagaaaaaat ttactcgata gcatatggtt aaatcctaag
    10081 tgttgcgaca atgggcaatc tgcagcgaag actcgaacag agtaacgttc aacgactatt
    10141 cctcttgagg gaagtacaca caagcgtgtg gaagtggttt cgccgtactg ggtaaagcca
    10201 acggataaga tatagtctgt gctggcacga aagtgtcaga agttcatcag agaactgcat
    10261 aggaagtagc gaacctatgt gaacaaactc cacataaaaa attcttcatt cttttgtgta
    10321 tattaacatt gatttagtac atggcttaac ctaaaattaa ggtatggata acgaatacag
    10381 atactcaaag acaactgtat cgttagtcaa ttaccacttt gttttttgcc caaggtatcg
    10441 tcggaaaata ttcgttaatt ctgaagtaga tgctagattt aaagcactcg ttcaagaaat
    10501 agcaaaggaa aacgagtgga acatcattgc aatagaaaca gataaagatc attgtcattt
    10561 attaataaat gttcagccta cgtattctcc ttctaaaatc atgaagataa taaaaggtaa
    10621 gacatctagc ataatccggc aagagtttga acacttaagt aaaatgccaa gtgtttggac
    10681 acgttcatac tttgtttcaa ccgcaggaaa tgtatctagc gaaattatca agcgatatgt
    10741 tgagaaccaa agaacaagat attgattgcg ttaaggaggt gtgcagaaat gaaaaaggct
    10801 cataaaatca ggatttaccc caactgtcgg caagaaattc aatttcaaaa aacattcgga
    10861 tgcgttcgtt tctattggaa ctttctacta gatcaacgaa taacgaatta caagaagaaa
    10921 aaagaaaatg ccgattacaa agaagataag acaacgtatg cttcattgaa aaaaatggaa
    10981 gaatacgctt ggctaaaaga agttgaagct caaccactta gccaagtagc catggatcta
    11041 aataaagcat ttaaaaatac gttcaactct acttttggat ttcctaaatt caaaagtaag
    11101 aagtattcca aaaagtccta tcgaacagct atgggtataa aggtgaatag aaactacttt
    11161 tatgtatcca aggttggctg ggtaaaaatg gctgaaacgt tgcgtttcaa tggtaaatta
    11221 atgaatgtga caatcagcca gtcaaaaaca gaaaaatatt ttgcaacttt tttagtcgat
    11281 attgaaaact tccaaaaaga tcctgtgacg gaatccattg gtttagattt gggtcttact
    11341 catttctgca taacttccgc aggtgagaag ataggaaacg agagattcta tcgatcatta
    11401 gaaaaacgct tagtggtaga gcaacgtaaa ctttcaagac gcttagaagt agcaaagaag
    11461 cataatcgta aattagaaga gtgcaaaaac taccaaaagc aaagagtgaa agtggcacgt
    11521 atccatgaaa agatttttaa ccaacgaaat gactttttac acaaactgtc ttctcgtata
    11581 actgatgaga accaaatcat ttgtattgag gacttgaatg taacaggcat ggttagaaat
    11641 cataaattag ctaaatcaat agcagacgta tcttggagtg aatttgtaag gcaattaaca
    11701 tacaagtgtg aatggaaagg tcgaacgtta gtaaaaatcg accgttttta ccctagctcg
    11761 caaatatgtt cttcttgtgg tcataatgac ggtaagaaag agttagatat tagagaatgg
    11821 acttgtagca attgcggaac aaatcatgac agagatatca acgctagtat aaacattaga
    11881 accgaaggac tttcggggat agcttagtaa atttgcgccc gtttgggtgt attacctaag
    11941 aaccccatga ctttagtcgt gtggagtgtc agggtggatt agttgatacg gataaagacg
    12001 gtgtcaaaga agcagtatgg gtagacaatg tagcaaatgc agcgatccta aaatcagata
    12061 aagaagggcg ctttgaaatc actggtttaa ctgaaggtga atattcattg gaagaaacaa
    12121 aagcaccaac tggttaccaa aaattaacag aagatattgc atttaaagta aacaaaaact
    12181 catttaaaga ggaaaatcga atcactgtta aaaataatca aaaagcagct attccattaa
    12241 caggtagtaa tggtttccaa acgtatgtat tgatctcttg cttgttgcta ggagcagggg
    12301 cactttctgc agttgtttac tttaagaaga aagcctagaa ggatcagcta atgattattc
    12361 gtaagccccc aaaaagaaga gtcatgcaca aactgatgat atttttttgg ttgctgtcgg
    12421 cagtagcagt ctatttaatt ccagttgtgg aaacaaatgt agctaaattt caacaaaagc
    12481 aaagaatttt agccattgaa aatcttgaaa aaacagattc ttctttggat gttgcgaaaa
    12541 tagagttagg cgatcctgtt ggtgttctta caattccaag tatttcttta aaactaccaa
    12601 tttatgacgg aacttccgaa aaaattttag agaatggtgt agggattacg gaaggaactg
    12661 gcgatattac aggaggaaac ggtaaaaatc ctttaatcgc agggcacagt ggactttata
    12721 aagataatct tttcgatgat ttgccttcag taaaaaaagg gcaaaaattc tttattaagg
    12781 taaatgaaga acaacatgcc tatcaaattt atcgaattga agaagttaaa gcagaagaat
    12841 tacaaaagaa ttatttagat tatctcatgc ctgaaaaaga ccaagatagg attacgttaa
    12901 tgacctgcac gccaaaaggt gtcaactcac accgcttttt agtgtatggt gaacgagttt
    12961 cattcagtaa agaagattta aaactacaga aaaaagcaaa aaataggaaa tcaatttcat
    13021 tggattggcg attagcactg ctattcgctt tttttgcgtt cctattattt ttatttataa
    13081 aaaaaataag gaggagatcg gaatgaataa gagaaaatgg ctgtttgttt gtgtagcagc
    13141 tctctttgca actgcagctt actcacagat atcttttgct gatagcagta ccactccaac
    13201 taccagtcag acaacagata gttcaacaac tgcaacaagc gacacggaca gttcgaagaa
    13261 cactgcagaa gaaacggcag attcaagtac gagttctact gaatcagcgg aatcaacgtc
    13321 agaaagttct tcagagactt cagaaaaact gcaagaagta gaagatacgc aagtaatcaa
    13381 gcaaaacatt catattcaaa aaattatttc ttcgaatcga atcaacaatc aaggaaggca
    13441 agtagctcat caagaaggtg tcaatggcgc aaagtttatt gtctacgata tcacgaatat
    13501 cttagaagaa atgacacaag atgataatga gaaaaatcaa tcggtagaaa taattgagtc
    13561 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tgactgaagg
    13621 agaaaccaaa acaattgatg atcaggaagg tgtgattgat ttttcaattg aagtacctgc
    13681 aaacaaaaaa caagcatact atatcgtcaa tgaatcaagc cctgaaaaca tttcaaatag
    13741 cgaagatatt attttattga cacctatttc agatgaaaag ggtgaatttt tgacggatgt
    13801 ttggttatat cctaaatcaa agaaatcaga gccgaaagaa gaaattaaaa aagttgtatc
    13861 aactggtgta aagaaaaact tttttgataa ttgttgggat tttttcactc atttattttt
    13921 taacgattag gatgaaatga tgaaaataat aaaaatgtta gcttttaatc tatctttttt
    13981 agccgcaaca atattgtgtt gcggtttagt tttttctcaa ttatcaattt atccaagata
    14041 tcaacgattg ttcgttcatt ttctaaatat ccagcatgct tccatgccac tagaagtgat
    14101 taacatgctc ttaatcaatc caatttttat ggctagtttg tttcttcttg caggattggt
    14161 aacgtgggta ttaggaaaaa aagaatgaga agcttacaat cgtttaacaa aataatagat
    14221 ggagggcatt atggaaaata aaattcattg cactacggga agagtattta ctttagtgtt
    14281 gtttttgttc cttcttcttt ttgcaagggg aactgcagtc caggccttag aaaaagttga
    14341 tccgagtcaa atcagagaaa ataataatct ggtactttat catgatagtg attcattgag
    14401 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
    14461 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agcacgtcaa aaattgttgc
    14521 ccattatcaa aaggtgggca cttatctagg aaaagaaatt gattgcgacg taactttttc
    14581 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatgggaa
    14641 aaagtattat aattatttgc tctttgataa aaacatttat cgaggctttt tcatgcagtc
    14701 aatcaacgtt atgaatgtaa aactagattt taagtattca ggatcaaaaa caaaagtagc
    14761 tttgagtgaa ccacagccag aaattaattc aaaaatgaga tcgatgagag cagctggtat
    14821 tgagttgaat gatacatttc tatctattta cagtttaaat ggacagtatc agcaacgtgt
    14881 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
    14941 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
    15001 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
    15061 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
    15121 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
    15181 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
    15241 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
    15301 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
    15361 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
    15421 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
    15481 agcgaatggg acttggaaaa aagctgatac aggaggaaaa ttaactgtat caggtaatac
    15541 agtcacttat actgcttctg acagcttttt agataacgct gcaaattacg gtagggatta
    15601 tattttgcag ctccatatga aagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
    15661 tactgtaacg tttaacaatg atccgaacaa aggaatacaa aaaacaaata cagtagaaaa
    15721 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
    15781 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
    15841 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcgtcagatc attacaagca
    15901 atttagtatt caagatccag tggaagctcg tttgacgtat aaagaaggtt ctgcgcgagt
    15961 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
    16021 tttaagaacg ctgaaatggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
    16081 acgagaaatt caactgatat ttacagctaa aactccacta cagtcagaaa aaaatattga
    16141 taaccaagcc gtagcagcag tagagaatgt ttctaataaa acgaatgttg taacaattgg
    16201 tgttgatcct aacttgccac aagtcattgt tcctagaaca ggttctacac atttagtaac
    16261 aattttagtg gttagcttag tattacttgt gttagttact cttagttatg tggctctgaa
    16321 gttcaattaa acaaaaaaac aaggctgaaa atcagccttg tttttctatt tgaaaattgg
    16381 attggcacta ataaatacgt tgtctgaaga aagtgatcca tctgcagaaa cttcataagc
    16441 atgagataac ccaacgatgc tttgattgtt gtaaagtaag tttgcgttat gagcagcgct
    16501 atctttccac ataatcatgg cattcactgc tcccgtatct gaaaggatct cgtcaacgta
    16561 aaggtagaaa ctattgatat tttctccttt agcgccgtag cagcctaaac tttctaagag
    16621 atcttggaaa gtaccgccgc caggtcgata atgactgaag cttacttgta attcttttgc
    16681 acgtatatca gcagcttgtt gaacgatatc attaaattgc attggagctg caccgttttg
    16741 ttgacgataa ttatttaacc atacaagttc tttttgacgc acatagttgt ttaaatcact
    16801 taatgagata tttttgtatt tgaacgtgat cgttcgattt tggtcaacat tattgattac
    16861 tcgttggccg tttttatcta cgctaatgga aggatcgtca tttctacaca agaaataatt
    16921 ttgatcagtc atatttttcc aaggagtaga gaagctatcc ccttcgtata ctgtttttga
    16981 ttcactagag agaacagcgt tggtatcagc atttacatga ttaacagtca cattaaattt
    17041 acgagtgtag ttgaaaatat aattttgact ttcagtcaca ttgtttaagg ttactgaatt
    17101 tgttggttct acccgatcgt ctaaaacata tccttgaata gcaggagcag ttaaagtgac
    17161 actttcaccg tgagtgacgt tgattatctg ttctttcaaa atatcaactc gcccctcatt
    17221 tttgtagttg ttattgcaca acaaattgat ttgatgcact tgattataat tgaaggtgat
    17281 tgttgtatta cctgtgatcg tttggctttg agtatcatta ccaatcactt tgtagctaaa
    17341 attatcttta gcgccttcgc tatatttaaa tgcttttgcc ttagcggtgt aagtagaacc
    17401 agaagtcact tgtgcttgat ctgtagcaat cacttgacct gtttgattgt caatatggtt
    17461 aatggtaacc atatatggcg tttcaggttt gtgttgcaac cctttaaatg cagcatctag
    17521 actattttta gcattattta cttgggcttg agaagcctta gaatcgtcta gtactttttt
    17581 agcattggat aaagcagtct gaaaatcatt ccaagattct tctgtaaaat ctcctttagc
    17641 agttgatttt actcggttgt ataaagcttg taattcagct ttattcacag taggtgctgg
    17701 ttttttcttt aatcccttgt aagcagaatc taaattattt ttagcactat ccacttgttt
    17761 ttgagtcgct tttgagtcat ttaacacttt attagcatta tttaaagcag tttgaaatgc
    17821 gttccaggta ttgtcggtat agtcaccttt tttcgttccc ttaacgctcg tataaagagc
    17881 ttctaaagct gatttattca ctttgggttt agaggtgtca gcgccatacc aaccgatacc
    17941 ttcgtatttc cagccatagt ctttggttaa ccattgtact tctttccaac ttgcagtgta
    18001 gtgatgtgag ccagatttta caccaggaag gaaaaggcgg tagatcggtt tattattttt
    18061 ttcggcagaa cgccaggcag gcccttcata agtccagccg taatttttag tcaaccattt
    18121 cacttcatcc caactagctg tgtaatggtg atcacgaaga cctttattgt ataaacgata
    18181 gacaagatga ccaccgctag tcggtgcttc ccaagcgggg ccttcataat tcccccactt
    18241 aatcttcact aggtgatcac gttcttttgt gctagaggtg taaaaatgtt catggttatt
    18301 tggattataa aggcgataca tgacgttagt tgaagcagca tcagcagatc cagcgaaacc
    18361 aagtgtggca gcaaatgttc caatagctaa aacagttttt gggtttaata atttcatatt
    18421 ttcaactcct aaaaattagt taaaatcgat tacaaaatca ttatatatcg gtaaaccgat
    18481 atggtcaata aaaaaattaa gaaaagcctc atgtatttct ggcttttctt aatttgttac
    18541 ttagttgaag cgttagtatc aatggtttct tcagctaatt tatttaattt tatagcattg
    18601 atgaatctca tattgtctat actagagcgc ttatttgcga gatcactgac cgtagttaaa
    18661 ggtacgccag attctataga tatagaacga actgatttac cactatcaaa cagcaaccat
    18721 tcgattttct ttttatcagc gtacattgca atgctccctc ttgatttttt ctttattata
    18781 gctctttttt gtaataaatc acaatatcaa gagagaaagt ttttatagaa aggtgggaaa
    18841 ttatgagtat ccgttccccg tgtatcaaaa tgaggtttgt tgctactttc gttatttgat
    18901 atactaaaat aaaacaacga aagaagaaat aaaatgaaga aaaagcaact atggtttgtc
    18961 tctatgtttg caatattttt tatt
//
LOCUS       DACEUF010000050        18504 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.053, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000050 DACEUF010000000
VERSION     DACEUF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.053"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            99..746
                     /locus_tag="IXG20_001721"
     CDS             99..746
                     /locus_tag="IXG20_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1609918.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IXG20_001722"
     CDS             1139..3784
                     /locus_tag="IXG20_001722"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ1609919.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IXG20_001723"
     CDS             4094..5407
                     /locus_tag="IXG20_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ1609920.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IXG20_001724"
     CDS             5397..6050
                     /locus_tag="IXG20_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1609921.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /locus_tag="IXG20_001725"
     CDS             6105..6797
                     /locus_tag="IXG20_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ1609922.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="IXG20_001726"
     CDS             6947..7147
                     /locus_tag="IXG20_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1609923.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="IXG20_001727"
     CDS             complement(7401..9080)
                     /locus_tag="IXG20_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1609924.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="IXG20_001728"
     CDS             complement(9082..9294)
                     /locus_tag="IXG20_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ1609925.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="IXG20_001729"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="IXG20_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ1609926.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="IXG20_001730"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="IXG20_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ1609927.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="IXG20_001731"
     CDS             13269..13466
                     /locus_tag="IXG20_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609928.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="IXG20_001732"
     CDS             13619..14104
                     /locus_tag="IXG20_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ1609929.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="IXG20_001733"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="IXG20_001733"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ1609930.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="IXG20_001734"
     CDS             14981..15508
                     /locus_tag="IXG20_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ1609931.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="IXG20_001735"
     CDS             15599..16336
                     /locus_tag="IXG20_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1609932.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="IXG20_001736"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="IXG20_001736"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ1609933.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="IXG20_001737"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="IXG20_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ1609934.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3200 c         4022 g         5298 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DACEUF010000051        17687 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.054, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000051 DACEUF010000000
VERSION     DACEUF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.054"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(136..1716)
                     /locus_tag="IXG20_001738"
     CDS             complement(136..1716)
                     /locus_tag="IXG20_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ1609935.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2117..3493)
                     /locus_tag="IXG20_001739"
     CDS             complement(2117..3493)
                     /locus_tag="IXG20_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1609936.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3621..4739)
                     /locus_tag="IXG20_001740"
     CDS             complement(3621..4739)
                     /locus_tag="IXG20_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1609937.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4881..5315
                     /locus_tag="IXG20_001741"
     CDS             4881..5315
                     /locus_tag="IXG20_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1609938.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5393..5926)
                     /locus_tag="IXG20_001742"
     CDS             complement(5393..5926)
                     /locus_tag="IXG20_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ1609939.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="IXG20_001743"
     CDS             complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="IXG20_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ1609940.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7191..7988)
                     /gene="recX"
                     /locus_tag="IXG20_001744"
     CDS             complement(7191..7988)
                     /gene="recX"
                     /locus_tag="IXG20_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ1609941.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8095..9477
                     /gene="rlmD"
                     /locus_tag="IXG20_001745"
     CDS             8095..9477
                     /gene="rlmD"
                     /locus_tag="IXG20_001745"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1609942.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9651..10229)
                     /locus_tag="IXG20_001746"
     CDS             complement(9651..10229)
                     /locus_tag="IXG20_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ1609943.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10551..11021
                     /locus_tag="IXG20_001747"
     CDS             10551..11021
                     /locus_tag="IXG20_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ1609944.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11129..12355)
                     /locus_tag="IXG20_001748"
     CDS             complement(11129..12355)
                     /locus_tag="IXG20_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ1609945.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13109..13309)
                     /locus_tag="IXG20_001749"
     CDS             complement(13109..13309)
                     /locus_tag="IXG20_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1609946.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13443..14051)
                     /locus_tag="IXG20_001750"
     CDS             complement(13443..14051)
                     /locus_tag="IXG20_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ1609947.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14136..14606)
                     /locus_tag="IXG20_001751"
     CDS             complement(14136..14606)
                     /locus_tag="IXG20_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ1609948.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14654..15568)
                     /locus_tag="IXG20_001752"
     CDS             complement(14654..15568)
                     /locus_tag="IXG20_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1609949.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15714..16517
                     /locus_tag="IXG20_001753"
     CDS             15714..16517
                     /locus_tag="IXG20_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ1609950.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16695..17642
                     /locus_tag="IXG20_001754"
     CDS             16695..17642
                     /locus_tag="IXG20_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1609951.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5363 a         3543 c         2910 g         5871 t
ORIGIN      
        1 gacatttcta tgattttaat atagtaaaaa gagccgtcac ggaaaatccg tcacggctct
       61 aaacaaaata tgagaggctg tttttgtttg gctattctta ttacttgatg cagggcagct
      121 tgtgtcaaga cttctttaca tcaaactttt taattttaca ttgggatatt tatccgcaaa
      181 ccagcgttct gcaaattggt tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc
      241 caagatattg cgacttgaac tcatcttctc atccaaatct tctggatcga tccagcgagc
      301 gattttgtgt cccattggag acatgattac ttcggcgtta tactcattta acatacgata
      361 ttggaatact tcgaattgta gctgaccaac agctccaatg atatattctt ccgttagata
      421 tgttttataa agctgaatcg ctccttcttg aactaattga tagattcctt tgtggaagga
      481 tttttgtttc atgacatttt ttgcagtcac tttcataaac agctctggag taaatgaagg
      541 gagttcttca taagcaactt ttagttttcc ttcgaacaat gtatccccaa tctgataatt
      601 gcctgtatca tatacaccga tgatatcgcc tgctactgct ttttcgacgt tttctctcga
      661 atctgccata aattgtgtga cattgcttaa tttcagtttt ttatttgtac gttccaacca
      721 gacatccatt cctcgttcga atgttccaga acagattcgg acaaaagcga ttcggtcacg
      781 atgggctgga ttcatatttg cttggatctt gaatacgaaa cctgaaaatt cttcctcata
      841 aggacttacc atctgatcat cttgtgtttt gtgtccataa ggagctggtg ccaattcaac
      901 gaaagtctct aaaaaagtct gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg
      961 tgtctgatcg ccacgagcaa ttttttcttg attaaatgca tctccagcct ctttgattag
     1021 ttctacttct tctaagactt gttcatataa ggaattttca gttagaggat gattttcggg
     1081 aagcagtcca tcttcatcca attctactaa tcgttcatcc tgataatttt ccggacgata
     1141 aaactctaca cgattatggt agatatcata aagaccttct agccctttac ccataccgat
     1201 tggccaattc atcggataag attcgatatc taatagctct tctaattctt ctaacaattc
     1261 caatggttca cgaccatcac gatctagttt gttgataaaa gtaaatatcg ggatgccacg
     1321 tttttttaca acttggaata atttctttgt ctgtgcttcg atcccttttg cactatcgat
     1381 taccatgacc gcactatcca cggccatcaa agtacgataa gtatcttcag aaaaatcttc
     1441 atgtcctggt gtatccaaga tatttactcg tttcccttga taatcaaact gcataacaga
     1501 actagtaaca gaaatcccac gttgtttctc gatttccatc cagtctgatt tggcaaaatt
     1561 acctgttttt ttgcctttta cggtcccggc ttgacggata gccccaccaa acaatagtaa
     1621 ttgttctgta atcgttgttt tcccagcatc cggatgggaa atgatcgcaa aagttctgcg
     1681 attgtccact tgttgcttta attttggatt attcataacc taactccatt tcttccattc
     1741 aattatttat attttctcta ggcacagatt gtccgatctc ttaaagaaag acaatttcat
     1801 atagggctgt gacaaaagcc gtgtagcagt ctctttttcc gagtaaacgg cgttctagag
     1861 acagcctatc agcaataagc ccacccaagc caaaaatacg aaaagctgtt tttgcttggc
     1921 tgctcttatt acttaatgca ggatagcttt ttcacaacct ttgtcaacta ctacaactga
     1981 atcagtatag catggattca gcgaatgaaa aagccaccaa accataagag tcaaatattt
     2041 ttttgtcagc tgactaaaag aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc
     2101 ataaccaagt ttccaattat cttcttggct cgtcatcttc gaattctttt ctgaagtatc
     2161 ttcgttcttc ttcaggttcc gcttctgttt cttccggatc atgaaaaatg acacggattt
     2221 taaggattcg agaaccttcc atttcttcag aaaccagcgt aatattatcg acatcaaacg
     2281 ataacttttc gccttcatcc ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg
     2341 tatctacatc actcatatgc agatcactgt caaaagcttc attgaactca tcaatcagca
     2401 tacgtccttg gatgatatat tcgtgttcat cgattttttc ataaagattc tcaacttcat
     2461 ctgattcatc atcgatttct ccgacgattt cttctaaaag gtcttctagt gttgctaacc
     2521 caacgacgcc gccatattca tctaataaaa tagccatctg attctgcgtc tttttcattt
     2581 catataacag atcatcaata aagaccgttt caggaacgaa taaaggctcc tgcatgattt
     2641 tcttgatatc taaattatca aagccgaatt tatgggcagc ttttagtaaa ttctttgtat
     2701 gaaggattcc gactacttta tctttgtctt cgttataaac agggattctt gaatagtttt
     2761 cggataaaac ttcattcaca ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat
     2821 ccgttcgtgg gaccatgact tctcgggcaa ctttcgtatc aagagaaaaa accccttgga
     2881 gcatttctaa ttcttcatta ttcaatacgc cttcgttttc aagcatataa cgcatttcat
     2941 ctctggtcat ttttgaatct tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac
     3001 ttgtggaagc agaaagcagc cagacgaagg gcttagctac tacaccaatc aagcgtatca
     3061 gccctgaagt gaactttgcg acttcttctg atttatttaa agcaatacgt ttagggtaca
     3121 attctccaaa aacaatcgat acataggtca aaatagccag cacaatgata tttgcaatat
     3181 tttttgccgc tgtgccgcca cctaataccg gagctaaccg ttccgataaa gtattagcta
     3241 gtgaagcacc agacaaaatg ttgaccaaag tgattcctac ttggattgtc gataggaaat
     3301 tatttggatt ctgcaatatt tttaaaagct tttgagattt cacatctcct tcatcagctt
     3361 tttgctcgat tcgattctta ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag
     3421 cattgatcaa tgtcaaaacg atcaataata aaatctgcgc taacagcgac tgactctcag
     3481 ggtcagcatt cataatcgca ttctctcctt ataaaattaa tttttagttt acgatagaaa
     3541 aaaacgtggc actatagcca cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa
     3601 aacaagtgta ctaaaactac ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc
     3661 ttgtcttctt tgacgatttt gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa
     3721 ggaacaccta agaagatacc aagcaatccg gcaatatttc cagctactag aagaacgata
     3781 atgatcgtta atggatggat tttcagtgat ttcccaatta cattaggata aatgatattc
     3841 ccatccaact gttggacgat tagcacaacc acacaacaaa gtaatgcttt taccggatca
     3901 ttgaagactg ttactagaaa tgcaggtgct agtcctagat aaggtcctaa atatggaatt
     3961 aggtttgtca atcccgcaat gaccccaaat aagaaagcat aatccacacc aattaccata
     4021 tatcctaaaa atgtgaaggt accaacgaat aaacattcga ttgcttgtcc gctaatgtaa
     4081 ttagccaatg ttttattcag ttgccccaat aactccacaa tttggtttct tcttttttct
     4141 gggaaaaact gttggatgtt tggcactagt ttttctccgt ctttcaacat atagaacaag
     4201 atgaatggtg ctgtaatgat tactacggtt gccgaagcga tcgttccaac gatcgaacct
     4261 aaactattgg atactccact aaggaattgc tgaatgattg tcccgtaaga aatgtctaat
     4321 tgctcaaaat atttattgat atctacttcc ttgaacaaag gcaattcggc tacctctttt
     4381 aaccagcttt ctacctgttt gatgaaatca ggcatattag atgccaatga ggaaatttgc
     4441 tgtactagac ttgggatgac actaagtaaa atccaaataa ttgctgcaac taaaaggagc
     4501 agcacaattg cgacagccca tattcttttg atttttgctt tcatcaacag cttgacaata
     4561 ggattcaata aatagtaaag aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa
     4621 aatgtcccga tcggttcgaa aataaagttt atttctgatg aaatcatgat caatgcagct
     4681 atgatcaata attcaagcga ccaaaacatt agtttagact gtctaagttt ttcaaacaat
     4741 agatttcctc ctttctttct tactagtata tattatattc gttaaaaatt cttttattct
     4801 ttttttattt ttgaaaactt gccatacttt ttatttgttt tattttccag tatactatac
     4861 gaaaggaagg tggctagacc atgaaagttg tacaaacaaa agatacaatg agcaatattt
     4921 acttagatgc tgtacggatc aggcatcaag tttttgtagt cgaacagggt gtgcctttgt
     4981 ctcgagaaat agacaaagat gaagcccatt gtatccactt cgtcctgtac tcagataaaa
     5041 aagaaccgca aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc
     5101 ggatggcgat tctttctgaa tacagacacc aagggttagg aaaaatattg attgaagaag
     5161 cagaaaactt tgctaagaat caaggctata atacgatttt attaggggca cagtccacag
     5221 ctgaaacttt ttacgaaaaa ctaggatata cagcttacgg cgatcctttt gaagatgctg
     5281 gcatgcctca tatttatatg aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa
     5341 acagcaagct tttgagaaaa atctctcact ggcttactgt tcttttttgt gtttacttac
     5401 gcgacaattg cttgtaacgg ttataccaaa tatcaatata gccttccgag aacgggccat
     5461 ctccattatt gatccagtca accagaatct tcacattttc ttttaggatg aagtcaatat
     5521 cgttcgggta attcatcatt ttgctgtgca tctcatattc atcgacatcc aatagtcgtt
     5581 tctcgccgtc aggaaatacc ttgatatcca agtcatagtc gatatatttc aaggcctcat
     5641 cgtccagcaa aaaaggtgaa gctagattac aataatagga aacccccttt tctctgatca
     5701 ttgctattac attgaaccag tattttttat gaaaataaac aatcgctggt tcgcgcgtta
     5761 cccatcggcg tccatcagat tcagtgacta atgtgtgatc attcacgccg atcatagagt
     5821 gttcgcttgt ttttaatacc atggtatctc gccacgttcg atgcaatttg ccgtcgtgct
     5881 tgtaactttg gatcgtcaca aactctcctt cttttggaac tcccatagca acccttcctt
     5941 aagcagcatt ttgtgctttg tgtttgggtg ttcattacac cgacatcata attattatat
     6001 caataaaaat gagctttgcc tagaaaggat tcctattgtt tcgatttttc tctaactgat
     6061 ctaccagttt catttggacc ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc
     6121 acttcgaagt tttgttctcg gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa
     6181 aaaggagaac atgccatttc agatgactaa aaatatgagt aacttctcct aaatgtcgtg
     6241 tctgccaaac aaagagttcc tgcttttcaa atgggagatt ttgctcttct tctgctacta
     6301 ggtcatcaaa cagatcgatt tctggctttg tttcccattc ttttttcaat cgctcatact
     6361 cctcttgtgt cacttccatc attggaaatg tccacatatt cgccagcaat ttttggctat
     6421 ctctttcttc aaaataatac gctccagaat gattttgaag agctgcactg atataataaa
     6481 cgtcttttgg ctttgctttt tttgttttta caggaaaact cgtttggatt cctcgtttgt
     6541 tagctaaaca aaaggcttgg atcggacaag tctcacactt tggagaagtt ggtgtacaga
     6601 tagctgaacc tagatccatc attgcttggt tgaattcacc tggatgcttt tcatcaatga
     6661 ttttccgcat cgcttcatca aagattttcc tgctagaagc ttttgcgatg tctgcttcaa
     6721 tgcaaaacaa tctgctgact actcgcatca cgttgccgtc aactgcaggt tctggaagtc
     6781 caaatgcgat acttgcaatc gcgcctgtcg tatatggtcc tattcccttc aatgagctaa
     6841 tttcttcagg cgtttgcggc atttccccat caaattctga catgatctgt ttagcagcag
     6901 cttggatgtt ccgggctctt gagtaataac caaggccctc ccatgctttt aaaagctttt
     6961 cttctggagc atttgctagc tcttcaattg taggaaacca ttccatgaaa cgataaaaat
     7021 aatcgatcac tgtgtctact ctggtttgct gaagcatgat ctcagagatc cagatacgat
     7081 aaggatcacg attatagcgc caaggcaggt ttcttttttc ttgttcatac cattgaatga
     7141 actgatcttg aaattctttt gtttctttat ctgtccattt ttcccattct ttattcactt
     7201 tcctcttcct tttccgtaat gaatcgctgg atcatatcca aatcaaatcc tttttgatat
     7261 aagctttgct ttacttttag atttcttttt ttaggttcaa accttaaatt tcttcgccat
     7321 agtttatctc cttgtttgac taaataatca tattctattt cttgatctgc ttcttgcgga
     7381 agatcagcta tcgcttgttg gatcacttct tgagaaaagc ctttcgtcat caatgtttgt
     7441 tggatttttc tcaataattc tttttggctt tttccgtgat tttttccaaa cgctttttcg
     7501 gctgtctgat gtgcattttg aacttggtct tcaaatgtat attggctgag cgcttgttca
     7561 atgatttcag gtttcaaacc tttttgctgc atctgctgcg ctaatttctt tggtcctttg
     7621 tcacttaagc gcatattcgt gcgcacataa ctttccccat agaccaaatc atcgacaagg
     7681 tccaattctt ttaagcgggc tacgatttta tttcgatctt cttgaaggat ttccttttct
     7741 tttaagtatg ttcggatttc tttttccgat cgcaactgat aacttaagta attcattgct
     7801 aggtgaaaac cgaaatcagc tccgctactc ttcttgatct cttggatagt ttcttcgtct
     7861 aattcccgtc cttttaataa tcgatagcgt actaatacat cttcagatac ttgtaaagct
     7921 tctccttcag ataaatctac gcgatagaaa gggcctttgc ctttactgat tctcgtaacg
     7981 gttaacataa acatcctcct cgaacatata ttctctatca taccacagaa aatccccgta
     8041 atcaggtatt atatgttaga ataaaccaca gaaaaaagaa aaagggagat acttatggca
     8101 gaaacaattg tcaaaactgg acaaacggtt cctttgaaaa tcaagcgttt aggcataaat
     8161 ggtgaaggaa tcggttattt caaacggttg atcatatttg ttccatatgc actaccaaaa
     8221 gaagaagtac tagtgaaaat cacgaaagca actcctcgct atgcagaagg agaattaata
     8281 aaagtcaaaa aaacaagcaa agatcgcgta gtggcaccat gtcctgttta ctatgaatgc
     8341 ggtggctgtc aattacaaca cttagcttat caagctcaat tggattttaa aaaggatctg
     8401 cttttgcaag cattggaaaa attcaaacca gcaggctttc gatcttatca acttttgccg
     8461 acgatcggga tggatgagcc ttggcattat agaaacaaag cacaatttca gttacgaaaa
     8521 aataagcaga ctcaaaaaat tgaagctggt ttatacgaag caaactctca tcagttagtc
     8581 cctataacag actgcttggt tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt
     8641 caactattga ataaatttga tgcacccatc tatgatgaac ggacaaacag tggaatcttc
     8701 cgcacaatca tggttagaat cggtgttaaa actggggagt tacaagttgt ttttattact
     8761 cgcagtaaaa aattcccgca aaaaaacgca atgatccgtg agatcaatga acgactgccc
     8821 gaagttgttt caatcatgca aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat
     8881 gaaacgatcc acttatgggg aaaagaagcc attgaagaac aaatcaatga agtgactttc
     8941 gatctttctc ctcgtgcctt ctttcaactc aatcctcagc agaccgaatt gctttatggc
     9001 gaaggaatca aagcattaga tgtccagcct aatgaaacga tcgtggatgc ttattgcgga
     9061 gttgggacga tcggcttaag tgtagcaaaa caagccaaag aagtacgggg aatggatacc
     9121 gttcctcaag caatcgctga tgcaaaaaaa aatgcggaaa gattaggata tcacaacact
     9181 cgatatgaag tcgggacagc agagcaagtt ctgcctaaat ggttgaatga aggttttcag
     9241 cctgatggta tcatcgtaga tccaccacgt acgggtttgg ataacgcatt gatccaatca
     9301 ttattgaaat accctccaaa aaaacttgtt tatatctcct gtaatgtttc tacacttgcc
     9361 agagatctgg ttaatttaag taaaaagttc aagattgaat atttacagtc cgtcgacatg
     9421 tttcctcaga ctgcacgttg tgaagttgtc gtgaagatga cgagactcgc tgactaaatg
     9481 aaagaggttg tgaaaaagtc tgccctgtat caagtaataa gaacagccaa acaaaaatag
     9541 ctcttcatgt tttatgaata ctgtacttgt gaccgaagag ttaacaggag gacactattt
     9601 atttaaaaaa agggttgtga catagttttt gtcacagccc tttttgatta ttattggata
     9661 ttcacgtcta tcacttgata aaatgcattt gtcgtatcgc tgatattcca tactgctaaa
     9721 atcacattat atccttttcg atcctgcgga agtgtgatcg tttggttgac tgtttttggt
     9781 ggaacagttg ctttgtcgtc tatttttgtg atcaattcaa aatcactaaa tttcaaaggt
     9841 tgattaggat tccaagttgg tttggtaatg tagtaatccc aagtacttgt ccgatgttgt
     9901 gctgttaaat tccaagtaat atttaatgtt cctggattaa ccatgttttt atgccatctg
     9961 ttagatgtct gttcatctaa aggctcaaag ccgctgactc ctgcactagc aatttttcca
    10021 tcaatgaatg tatttttggt agcttcgata ctttgcggtt cccattgtgc tcttccgaca
    10081 ttttgattta agtttcctgc tgctgatgtt cctaggtatg cacggcttcc tggagaagta
    10141 atataaccat gcgcaaaacc tggaatgcta aaaccgaaca accctaaact aactaaccca
    10201 ataaacaata acgtactaat ctttttcatg tagaaacctc cttcatttga taaccacatt
    10261 gtaaacgctt tattgtatta acacaaacat tatgctttta taattttatg ttgaagtaaa
    10321 aaaggtgaaa aaagtaagat gcagattata acagaagtag acgagaaaag tacaaataga
    10381 aaacatgaga atcaaatcat atgatctttt tcaaaaacat cattcatata aacaaatgtt
    10441 caacttttta ataacaacaa ttatcaattt ttttcttact tcacaaacta aatacaggtc
    10501 ttgctgtgag ccttcatggt aaactactat tatagaaagg agttagtaaa atgaagatga
    10561 taaaagatat tcgtttttgg gtttgtgtta taggaattgt catattaggc tttttatcag
    10621 ggttgcttag cggaaatccc ggagaatatt actattcttt gcaacttcca ccatttgcac
    10681 cacctagctg gatatttggg ccgatgtgga ccttactgta tatattgatg ggtatttctc
    10741 tttatttgtt acttaatcac aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg
    10801 tgattcagtt catttttaat tttatatggt ctgctctatt ttttaattta agaaatattt
    10861 tcattgcagc aatcgatatt acacttttag ttattttctt gtcagtgctt atgtatcagt
    10921 tgtggctaca tcatcgcctg gcaatgtggc tcatgattcc ttattattta tgggtactat
    10981 tcgcaactct attgaattat tcaatttatt ttttaaacta agcgctaaaa agagacgtcc
    11041 tgtaatttaa cacatagaaa aagtgccacc taagttagat tttttgtcta atttaggtgg
    11101 cactctttat taaccaaact atttttttct agtacccact tgttgttcca tactcattat
    11161 tctggtcata accataacca ttttgttcct gtccagcgta aggatcataa ctttgagtat
    11221 cttgacttgg tgtatagtac tgacttgtat cttctgtttg ctgataatag gtattcggat
    11281 cttggtactg atcatatgtg ttttctgaat aggaagaata atcggtgtac ccttcactat
    11341 tcgatccatc accgtttgtc gaatcgccat cttccgaatc atcaaagaac atcattgaag
    11401 cattctcatt tttcaaatta ggtaatgtat cgcttgtttt tatatttaat tgtttcttca
    11461 gttcattttg gatcgaaaga agatcatttt ttcctaaaat ttgatagtag atatcattga
    11521 tcatttgtcc ttcgccttgc aactggtctt gtttgattgt ttcaaacgca ggggtgtagt
    11581 tcgtcgctac atcaagcatg tcatcccaat ctaagtccgt cgtcacattg ttctcaactg
    11641 cttttaaaat ttttctataa ttgcttacgc catctaaact taaaacttta ttgacgattt
    11701 tcgtcactac ttcccgctga cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc
    11761 gcattcgaga ataggctaaa cctttttctc cattcaatgt ttgttcacct actggtacat
    11821 ggacaccatc caaagtaaag tcaattttat tattcacttt gatgccaccg accgcattga
    11881 tcaaatcttc catgccatcc aaattgatcg ttacataatg atcaatagga atatcgagca
    11941 gttgctcaat tgaatccatg gccatttcga caccaccaaa tgcataggca tgatttaatt
    12001 tatctaccgt tccataacct acgatattcg tataaatatc tcggtccaaa ctgatgatcg
    12061 ttgatttttt ttgtttcgga ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt
    12121 ccgttcttcc taatccaccg gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt
    12181 catcaatgct tactctctca gaacgtttgg aggatattcg attagagttt ttagaaattt
    12241 tgtttactgt ttggctcgct tctcccataa atctgattcc gtatgctgat aatccggcaa
    12301 tcagcaaaac aaatacccct aacaaggtta atagaacttt ttgccatcgc ttcataatac
    12361 acctctgttt cacattttct aaaatattgt ctcatataac tgttcaatca gcaaattcac
    12421 tttttaatca taagaaaact ttaaaaaaca tttttgtgta acgattacta ttttttaaaa
    12481 tttctcaaaa ttcttaacta tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac
    12541 gcagttcgca atcgttttat ctttttttac gtattttttc cagaaaaagc gaacaacaag
    12601 gtattttcca aataatcgtg cagtaaaatg atcgcacata cctttcccat ctttttaaca
    12661 gtctttgcct accgttcaga ttatcattcg atttcacgaa aatttcttcc tatgactggt
    12721 ccttttgtta aacccatctt ctattcgttt tatttccagc tcactgcatg gctcttaaaa
    12781 tgactcgaac tagtgatttc attaccatca tcttttaatg gatagatatg ctcctcttca
    12841 aagtaaaacg attttctttt aaagtaatag aattgacatg gtaatgagta tgtatactag
    12901 tggtttcaca aatggtcttt tattcaacat ccaaaaactg aaatttcttt aaaatccatt
    12961 actaacgaag aaaatgttcc ttgcttgata attagttagg acatgtcaag tgaacaaatg
    13021 catttactta gccaaaaaat ctaaaaactt cataaataga caaaaagaca cgactctcta
    13081 agaagtcgtg tctttattca acaaattatt ataatttagt cacttcagca gcttgaggtc
    13141 cgcgtgcacc ttcaacaatc gtgaattcta cttcttgacc ttcttctagg ctcttgaagc
    13201 cgtctccttg aattgctgag aaatgaacaa atacgtcatc tccgccgtct actgtaataa
    13261 acccaaatcc tttttcattg ttgaaccatt taaccgttcc gtgttccata aatgtatagc
    13321 ctccaaaaaa gttcttgcaa gtaaccttgc aagaacatta tacccaattt agaaaacgct
    13381 agcaagcttt cgctctttta ttttccaaat tatttttaag actgatatga cggaggaaat
    13441 ggttattttg ctgcttcgaa acgtttgttt acttcatccc aattaataac attccagaat
    13501 gctgcaatat aatctggtcg gacattttta tatttcaaat aataagcatg ttcccaaacg
    13561 tccaaaccta gaattggtgt ctttccttcc atcaatggag aatcttgatt tgcagtagag
    13621 gtaatagcta attttccatt ttccattaca agccaagccc atccagaacc aaatcgtcca
    13681 gcggctgctt ttttgaattc ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc
    13741 gcttctttta tttctcctgt aggttcgcca ccagcatttg gtgccatgat ttcccagaaa
    13801 aatgaatggt tagcatgtcc gccaccattg ttacgtacag ctgtcttgat atctgttgga
    13861 atagcgtcca tatcagataa taattcttct attgtttttt cgcctaattc tgggtatttc
    13921 tcaattgctg catttaaatt cgtcacataa gtattgtgat gcttatcatg atgcagatgc
    13981 atcgtttctt cgtcaatata aggttccaat gcgtcatatg cataaggtag atctggtaat
    14041 gtgtaagtca taaaaaaatt cctcctcaat gttttcttta taatgtctac attacaaaga
    14101 gtaacaaaga gaaggaaatt cttcaattgt tatgcctagt cgtctttttc actttgacct
    14161 acttcttctt caaaaacctc agtttcttta aaaataaaca acttgctgaa aatatagttc
    14221 gctaggataa caataatttg agtgatgatt ttcgccacat actcattgct gttcataaca
    14281 ttgatcaaca ataccattgc ccccatgtcc aatccgtaag aaaggatacg gtagaagaag
    14341 aacttaccgc attcaataag tgcatgtgta aaattcggtg attttgaatg gaatacccaa
    14401 agtttgttcg ttacaaaagc aaacaataca gaacaaaacc acgaaataga attggataag
    14461 gccatactta aatcaaaata ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta
    14521 gccaaaccac caaaaaagag atagactagt atctcccaat atccttttgc ttctaaatat
    14581 ccttttaact gtcgatactg cttcatgagc caacctacct tattaaaaat tcttatttat
    14641 catactctat atttcaataa ctttcatctt tatttaaaaa aattaatttt ttttgatcct
    14701 ttgcctttgt ctctgcttgg atcgtcacat gtgtaatacc atattttaat tttaataaag
    14761 aaattatacg aggatatatc ttttcaatct cgcttagtag ctgatcttca aaatcaatat
    14821 gtccgtcgaa aataattatc ttttcgctat actgccaaat atggacatga tgaacattct
    14881 ttaccccatc gattgcttca atttcttgtt ccattttttc atagtcaata gcaggtgcac
    14941 tttgtatcaa aatagcggca gcttttttga tcaccattat cgcttctatc aatatatata
    15001 tcgaaatgat cagtgtaata actggatcga tccagtagat atcgaaaaaa cggatcaaaa
    15061 ttgctccgat aatcacactt accgaagaca gtgtatcact taacaaatgc aaataactag
    15121 attttatatt caaactttca tctgcttctt gtcgtaataa taatactgag aacaaattcg
    15181 cgagcaatcc aataactgct actgttagca tcaaatgact atttatcttt tctggtgaag
    15241 tgaaccgctt gaatgcttca acggttaaaa acaaggataa tatgatcaat atgaatgagt
    15301 tgatgaaagc actcaatatt tctaaacgct ggtagccata cgtacgtttt ttatttgctt
    15361 cttttcgggc cattttttgt gccccgtaag aaaaaataat tgccaatgta tcactaaggt
    15421 tatggaaagc atctgaaaca agagacaaac tccctgacat aatccctcca ataaattctg
    15481 ataaagtgat caccgcattc aaaaccacta ctccaaatag tttttttgat gatctctgtg
    15541 gctgtgtagg cactattcct tcctccattt tatttatatt ggatagtctt tagacaagaa
    15601 taacattttt ttgatatttc accaatcatt cagatgtttc cctaaagcac ttgtatttca
    15661 cttgatactt cgctagaatt gcaagtgatg acactactag atgaggagta cttatgacaa
    15721 caaaacaatt aattatcagt tcctttatac gattcaaaga cctaaaggag acgagaaatg
    15781 tcccattttg gaaagccatt gtctatcttt tagttttgag tataattatg gctttgccaa
    15841 tctcttttca agtttttcaa gtcttaaaca acattcaatt ggatgggcaa aagattgcta
    15901 ctcgcattcc tgattttaca atccaaaacg ggcagcttca aaccgaagaa aaagaaggat
    15961 tcatttacca aacaaactcc attatcttca catttgatcc agagggaaaa cgaacagaac
    16021 aggacatctc tactgatttg atggggaatt ttgttagtgt cgggatgtta aaaaacaaac
    16081 ttatcatagc tcttcctaat acaggcacga cttctgcttt gttgaacaat aaccaattag
    16141 aacttcccta caccaatgaa tctttaaaaa acttaacagg taaggagctc cgttcttttt
    16201 taagtaaagc atctatacca ctttggataa aagtcctgac atttatattt tctatttatc
    16261 ctagttttct gaatctgctg atcactttgc tatttaccaa cattgctgct tttctctatg
    16321 cacgctttcg actaacaaaa gctacttttc ttgattgttt aaaaacattg atctattcaa
    16381 tttcattgcc tactataatt gcaacaatct tgatggtctt tcttccttct tttgacacaa
    16441 gcgccttcat tgcttttgca ggaatcttta tttttgctca agctgtcaaa ggatgggcaa
    16501 aaatatcgat caattaagaa ataaagacaa ctcgttattt ctacttcaat agaccctttt
    16561 agaggttgtg acattttttt gtcacagccc cttttttatt tgaaaaatat ttctttctca
    16621 aaaaacaagc aaggcgcttc aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag
    16681 gcgggaaatt aactatgtca gaaagaatca tttatgcaga tcgtccattt cgtgatgaat
    16741 ttatcgaaga aatccatact atagcgcctg gttatatctt taaaacagaa ttacaaccag
    16801 aggatctaca aaatgtagag atcagtttgg gctggaactc agactaccaa gaaaaactat
    16861 tagcctcttc taccttgaaa tgggtccaat ctatctcagc tggtgttgat cagctacccc
    16921 ttgagacttt ttcaaagaaa catattttac tttcaaatgg tagtggcatc cacagtgaat
    16981 cgattacgga gcatgtctta ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac
    17041 aaaaagcaca gagtgacatg aaatggttag gttcatcggt ccattaccaa tctatccaag
    17101 gaaaaaaaat actaatcatt ggaactggcc atattggaaa aatgattgca aaaaaatgca
    17161 aagcattaga catgaactgc tatggaatca atacatccgg tcatccagca gaaggaatgg
    17221 aaaagacttt ttctttaaat caactaaagg aagttcttcc tgaagtagat ttcgtagtca
    17281 atatcttacc tctaacggag gaaacaactg ggctctttga tcagcatatg tttgaacact
    17341 ttgatccaaa atctgtattc atcaatgttg gccgtggtgc ttctgttaag acacaagatc
    17401 tggtgcaggc tttaaataac cagcaacttt cctttgcagc attagacgtt tttgaggaag
    17461 agcctcttcc acaagatcat cctctttgga aaatggacaa tgtactgatt acttcccaca
    17521 ttgcaggttt gacacctgat tttcaaaaga aattaatggc tattttttta accaatctga
    17581 aaagctactt tgccacccat gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt
    17641 aatagctgaa aacaatattt tcaaaagaac aaagagaagg tgacaaa
//
LOCUS       DACEUF010000052        17517 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.055, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000052 DACEUF010000000
VERSION     DACEUF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.055"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            100..2427
                     /locus_tag="IXG20_001755"
     CDS             100..2427
                     /locus_tag="IXG20_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1609952.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2553..2882
                     /locus_tag="IXG20_001756"
     CDS             2553..2882
                     /locus_tag="IXG20_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1609953.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2922..3926
                     /locus_tag="IXG20_001757"
     CDS             2922..3926
                     /locus_tag="IXG20_001757"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1609954.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3923..5352
                     /locus_tag="IXG20_001758"
                     /pseudo
     CDS             3923..5352
                     /locus_tag="IXG20_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5558..6730
                     /locus_tag="IXG20_001759"
     CDS             5558..6730
                     /locus_tag="IXG20_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1609955.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6892..7347)
                     /locus_tag="IXG20_001760"
     CDS             complement(6892..7347)
                     /locus_tag="IXG20_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609956.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            7936..8358
                     /locus_tag="IXG20_001761"
     CDS             7936..8358
                     /locus_tag="IXG20_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1609957.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8524..9717)
                     /locus_tag="IXG20_001762"
     CDS             complement(8524..9717)
                     /locus_tag="IXG20_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1609958.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            9941..10318
                     /locus_tag="IXG20_001763"
     CDS             9941..10318
                     /locus_tag="IXG20_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1609959.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10306..10644
                     /locus_tag="IXG20_001764"
     CDS             10306..10644
                     /locus_tag="IXG20_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1609960.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            10778..11221
                     /locus_tag="IXG20_001765"
     CDS             10778..11221
                     /locus_tag="IXG20_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ1609961.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            11483..12298
                     /locus_tag="IXG20_001766"
     CDS             11483..12298
                     /locus_tag="IXG20_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1609962.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            12308..12970
                     /locus_tag="IXG20_001767"
     CDS             12308..12970
                     /locus_tag="IXG20_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1609963.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            13175..14839
                     /gene="alsS"
                     /locus_tag="IXG20_001768"
     CDS             13175..14839
                     /gene="alsS"
                     /locus_tag="IXG20_001768"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ1609964.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            14852..15562
                     /gene="budA"
                     /locus_tag="IXG20_001769"
     CDS             14852..15562
                     /gene="budA"
                     /locus_tag="IXG20_001769"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ1609965.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            15692..16189
                     /locus_tag="IXG20_001770"
     CDS             15692..16189
                     /locus_tag="IXG20_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ1609966.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            16374..16634
                     /locus_tag="IXG20_001771"
     CDS             16374..16634
                     /locus_tag="IXG20_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609967.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(16996..17235)
                     /locus_tag="IXG20_001772"
     CDS             complement(16996..17235)
                     /locus_tag="IXG20_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609968.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         5944 a         3045 c         3474 g         5054 t
ORIGIN      
        1 aaaaaagccc caaactttct atacatcaat aaaataataa aaattaaatt tagaataacg
       61 tgtaaaggat tatttgatga ttttttattg agaggaggga tgaaaatgat taagaaaact
      121 caaaataaaa tccaacgtat atgtcaaaaa cattttgttt ctagactcaa agctgtatct
      181 aaaagaaaat acttacatcc cattatgatt tcgatcatct tgatttttac tttcttaggg
      241 agcttatcaa gtatttatgt aaacgtttat acagaaacgt caaattttgc taaccaaacg
      301 acagaggaga caaatagtcc accctattct gaaatacagt ccatagaaag caccaatcag
      361 tctagcaaca acgagacaac ctcaaccaca tcctcgactc aactgattga tccgacagaa
      421 aaagaaatca cagcagcaaa agcagcaggg gagaaagctt attcagaaac aggtagaagc
      481 cagacattta gtactgttgc ggccactgcg aatttcaacg gacataccat accagatgga
      541 tatacagtga tatatgccaa agatggagtc attgttgcgg ttaacggatc gatgagtgcg
      601 gataaaactg cgttacaagg cgtaaccaaa gcaatcgctt ttattgataa ccgtccagat
      661 atccataatc tgtttattgg ctccacagta aatggtacta ctatcacatt cccaaaaagt
      721 atctatatgg gggtggctac cgctggaaat acgatcgatt cagttacacc caatgcggat
      781 aaaagttatt cttttacacc ctcaacatgg ggagggcttc ctgccggagg aggtatgagt
      841 actttttcaa tcggtataca gttagcaaat gatattccat tcacggggta ctcgatcaaa
      901 aatgggcaga tcgttgatgg taagggaaat gtttcaaatt ttccaaccta tcaagcagca
      961 acggttcagg tcaatgcgaa tcggacagga actttttctt atacagtcaa tggtggagca
     1021 gcaaaaatcg gaacgtacgg gactacatta tctggaatcc atgtgggtga caaagtggtt
     1081 atcactccaa cagcagtaac gggctattct tttgcacaaa caaacggttc tttcacagcc
     1141 ctagaaagta acgtcgatac ggtgacctat acaggcaaac aaacaccaat cactattaag
     1201 tatcaagact attttggaca gatgattgct ccttctaaaa taatgaatct tacttacgga
     1261 agtgctgcac aagacctgac gactaatacg cctactatca acggatatac ctttacagct
     1321 gtttcagcga cagaaacaaa gaaccaatca gcaatgagtg ttttagctag tttagataca
     1381 aatggaaatg taatcgtaaa ggacgcaaat ggaaaacaga tttctgaagt catcctatat
     1441 tataagacaa atgcaacagt atcaatcaat gcaaatggca gtaagtacta tgatggtctt
     1501 tctgttgtac cgatcgttaa atatgccttt aatgataaaa cagaatcaac atccctttta
     1561 aacaatgatc tcagtattga tcaaatcaca tggaatccaa ctgatttcaa agcgatgaat
     1621 gaaaagggag ttgaagtaac tgcgcctact gaaattggaa cctatacgtc ttggcaattg
     1681 acaaaaagcg gactagctaa actagctgcc agaacaaact atttatttac tatcgtgcag
     1741 acatctgatg tctatacgat taagcaaatc agcggtgcgg tagagctcag tggcagtaag
     1801 acatatgatg gaaaagcagg tgtcccgtca atccatgtca agctagcaga gggtgtcact
     1861 agttcgctta ctccagtaca agtggcactt agcagtatgg attacacagc agatgcacaa
     1921 tcagcaaaaa atatggttaa tgctggcagc tatacaatta aactaacgaa tagcggaata
     1981 gacaaagtca aagcagcaaa tcccaagttg tcatttactg atatagccaa cacatcagga
     2041 acttatacca tctataaagc taacgcagtg attactgtcg atgacgctgc ctttaattat
     2101 gatgcccaaa gtcactcgat ttcggttgga aatctccacg ttaccggtgt tgtcttaggc
     2161 gaatcattgg attataccct aacagttaac agccggacag atattggatc acaaatagta
     2221 acgattagtt taggcgaagg aagtgtaaac aataattata cgattatcgc aacagatacc
     2281 gctaagttga cgatcaatcc gactctcttg cttccttcga caggtgggat aggtctactg
     2341 ccctttgtct ttttaggact gatctttatt ttttctggct tcttttattt tatccatcgt
     2401 aagaaaaaag caggtgaaca gcgatgaaaa aaacaaaaaa tgtacgtgtg gatatatttg
     2461 ctgttctctt ttttatccta ctggtctttt ttataggttc tccgactgtg tcagctagag
     2521 aatcacctat tacatttact ttgcataatc gaatgttcga aagcgataag atgccaagta
     2581 tcagtcaaaa cgacggaata aatatcccgg ataactttca aggagtaaaa gggttgaacg
     2641 gcgttacttt cgctatatat gatgtctcgg atgaatttta caagctgcgg tcagaaggaa
     2701 gtagtgtgga agacgcacaa agaaaactag cacaaaaaag tgatagtgaa aaaatactgg
     2761 cagaaaatgt gacaaaaaca gtagacgagg aagagggcat tgcttctttt agtgcatcgg
     2821 acaaagatga gcaaggtcgt gacgctgttt accgatttgc ggaaataaag acttcagatt
     2881 aagtaaaaga aaaatcagca ccatttgtcg ttgtgcttcc tgtgacagat tcatctaatc
     2941 ataaattgac gaccatccac ttgtatccaa agagcgaaca aaaaatacct gctgcactca
     3001 cattaacaaa aacagtagag aataagcaga cagattttgc agatggggat aaagttcctt
     3061 atctcatcac cgtaacaatt cctgagaaca tcaacgagat aggaacctat acgatcaaag
     3121 atacagctga tcctcagtta tggctagaac cagaaacgat cgattttttg attggtggag
     3181 acaagattca tacatttcac acacaaaaaa caaaacatgg ttttgtctta tcagattctg
     3241 gaaaagatct tagttcgttt gctgggaaaa aattaacgat ccgttaccaa atgacattga
     3301 aagaaaacag tgaaaaaacg acatttgata acgatgtacg tttgacgact gacaatcaaa
     3361 cagtagaaac acatcttgcc atccaaacag gaggcaaaca atttgtcaaa gtggatcggc
     3421 aaacgaaaca aaaactagca gatgctcaat ttctagtcaa aaaaaatgac caatacttag
     3481 cgcaagaaaa cgggataaat cattgggtag ccaaagatga ttcaagagca acgattttta
     3541 cttccagtaa agatggaaca ttttctgtcc atggattgtc ttttggaagt tacgaactag
     3601 tggagattaa accgcctaaa ggatatatat tgagtcaatc aaccattctc tttgaagttg
     3661 aaaaaggcag ttatgatccg cttcatccta taccattgga aatcatcaac gaaccaaaag
     3721 caaatccgac gaataatgga aaaacgccag ttcagatgca gacaaatggt ccatcaaatg
     3781 aaaaaaatgg aggaagcttt ccaaaaacaa gcgaagagat gcttggaggt ttctcaattt
     3841 tagggttgtt gctagtcctt tcaacaggga cagcttggtt ttataaaaaa caacaaaaag
     3901 gaaacaatag gagggaaata aaatgaaaaa catcaaaaca aaaatatgga cgtttttagg
     3961 aactgctatc atgctgcttc ctttcgttct cggcctagga actgcagagg tttctaggcc
     4021 gtatcaccta caccagaaaa tgttacggtt aatttgcata aattgaaatt cacttcagca
     4081 ccagaaaatc aaataaacaa cggaacggaa ttgacatttc cgaatagcga accgttgaac
     4141 ggcgttgaat tcaacgtata tgacatcact gctacgtact atccaagtaa agacacagca
     4201 gttccagcag acgctacacc cttcgcaagt gtaacgactt ctggtgaagg attagctaac
     4261 ctgactcttc caggtaaatc tgatgggaaa gatgctgtat acgtctttgt agaaacacca
     4321 aagcccggtg tcgaaacgtc gccaaatatc gtgttaagct taccctttta taacccgaat
     4381 tcatcagacc ctgacaaacc tttagacacg attcacctat atgctaaaaa tgtcatcaaa
     4441 gaaagcaata tcaaaatcca gaaagctggg aacttttcag gggtagataa gttagcaggg
     4501 gcgcaattcc agatcaaaaa ttcagagaat caattcgtga ccggttttga tgaaaacact
     4561 ggtgtcgctc tctatgatcc tgctgagtca gcgttgacat tcacaactga tactgaagga
     4621 tccatcagtg tcaatggatt aatagacggc aattatactt tagttgaaac aaaagcacca
     4681 gatggttatc aattaaccaa tccacaaaaa gaaactactt ttatagtaaa aaacggaaaa
     4741 ttgcaacaaa caaccggtat catccatggc gcagatgaag ccaatcttgg gaatccaaac
     4801 ggtgtagtca atcgggaaaa tacaactacg gataacacga ttactgtaga aaatgtccaa
     4861 aactacggtg atttccactt catcaaacaa gatgtgaaca cgcaagaaaa attagccgat
     4921 gcagaatttt ctattctaag agcaaaagat ccaactgtta aattaatgaa aaaaatagca
     4981 aatcttgcct caggagaata cccatatgct tggtcagatg aagtcacagg ttctgactgg
     5041 gaagaagtga aattgatttc tgattcagaa gggaaatttt caatcgaagg gctgaaacaa
     5101 ggggcatact gccttcaaga aacaaaagca ccagaaggct acgcattacc taagaatgaa
     5161 gctgcgtaca ctgaatttac agtagatgat gatccaacaa cttctgatga aacaacgatc
     5221 gataaccaac ctaaaggaat cttgccttca actggtggta tggggatcat cgtattcatc
     5281 ttggtaggta ctgctttagt tggaggagca gtgatctact tcaaaaaacg ccatcaagaa
     5341 acagaagctt aaactattgg aaacaaaaat catacgataa ataaacgata gtgattcaaa
     5401 tggggagaaa ggttgtgaca agtggtcggt ctcttggtat tcgctcaaca aacggcaaaa
     5461 taggaggaac tattttgccg tttgttgagc gaatactcag gtcaaggcgc tgatttcaca
     5521 acctcttcat atacatatta gaaaataggt ggacatgatg aataaaagca gaataagaat
     5581 actggacatc ttcatggcaa tcattttagt cgttgggatc gggatctttt cttatccttt
     5641 tgtagaagat tcactgaatg attttcttgc ccagcaaatg atcatccatt atcaaaagca
     5701 agcatcgaat aagaattcag aggaaataaa aaaacagcag gaaaaaatga caaagaaaaa
     5761 ccaacagttg gcagagcaaa acgtatcacc agggataggc agtttcaacc aagcagttga
     5821 tgcaaaagca ttaaaagatt tacctagtaa tgcctttttt atggaacata tgcttggagt
     5881 catcgaaatc ccaaaaatca atgtcagctt acctatattc gatcagacaa cggaaatatt
     5941 tttgcaaaaa ggaacgtcat tattggaaga aagttcttat ccgactggcg ggaaaaacac
     6001 ccatgccgtt ctttctgggc atcgtggact cccagaagca aaattattta cagatttgcc
     6061 taagttgaaa aaaggagacc agtttttcat ccagatcaat ggaaagacat tggcgtatca
     6121 agtagaaaaa atccaagtgg tcttacctga tgaagtagat tctttgggta tccaaaaagg
     6181 cagagatctc gtgactttgc ttacctgtac tccttatatg gtcaatacac atcgactgct
     6241 tgtgacgggg catcgtattc cttatcaagc aaaagaggca aaaaaagcga tccaaggaat
     6301 tgatcaatgg aaaaaatgga aattttttat ttggtttatc ggtatactac ttggatcaat
     6361 cggcttagtt tggctactca tcgcttattt agattctttg gccatcgcta agcgaaacta
     6421 tcctttatct ttttatgtaa agaacacaaa cggacgacca atcgaaggaa tggtcttttc
     6481 tgtaaaaacg ctcaacggca aacactatat tactagagaa aaagttccat ttgtgaaagc
     6541 ttctgatgaa tacggactag tgagattttc tgatttaaaa ggcaggaact atcgcttgca
     6601 acatgaggag ctcctgctta aaatccatgt aaaacataaa cattcgaaac agttttcaat
     6661 gaaattgaaa aaaggaagat ataaactgcg aaaagaaaaa gaggtgtact atttgattga
     6721 gaaagaatga atgaagatgt ccaaaaaagc agagggaaca gactaaatga aaaaagagag
     6781 aagctgacga aatggacgtg taaactgcta tttttggttt actatctgga acgccagctt
     6841 ctattttttt atgtttcaga agaattttat tattatagta taacttttga attagtttcc
     6901 ataacatttg gagcaagggg ttaaaccacg agcttgagct tcgcttaaag tggcaggggt
     6961 atacgtacca tttccacatt tatgtgtatg gtattttgaa ccggtcggtg tgacatagac
     7021 agtttgttca ttattttgct caggagctgc ggcttgtgcc tgctgagagg cctgttcagc
     7081 tgctgcttgc tgtcgcgcct gttctgcttg ttgctgtgcg attgcttctt gttgcgcttg
     7141 ttctgcttgt tgtcgtgcaa tttcttcttg ctttttccgt tcttcttctg cttcttttgc
     7201 tttgattgtg gaatcaattg cagcaagacg gcttgtcaac tcttgtttgt tttcaggtag
     7261 cgcttggata gctgctgaag cagttgcata gttttctttt gtaggatttg cttcagcttg
     7321 tgacaaagca gattccgctt ctgtcacttt tttttctagt gcagcttttt ccgctttttg
     7381 tttctcttct tcttttttct tttcaatttg ttcttgttct ttcgatgaat ctgccgtact
     7441 ttcggagatc gtggtactgc tgctggaagc ctgagctgaa cttgtgcttt gagtagtaga
     7501 cgtagtagca ccacacccac tcaacaaact tcctgcgacc aaaaacaatg caactaattt
     7561 tttcactaaa tttcctcctt aagtaaaaaa caaccgtttt caataaatgt ccttttcaat
     7621 tgtttactta cttgttataa taattgtgtt atgaatagtg attgaggaaa cttaatctaa
     7681 tcccgttttg atacacggga ttttttgcta tagttctgtt attaagtatg caataataaa
     7741 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
     7801 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
     7861 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
     7921 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
     7981 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
     8041 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaaaaa
     8101 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
     8161 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
     8221 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
     8281 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
     8341 acggacgtca aagaatagta gccgccagat agaaggaaat tagcagttga aaaagctgcc
     8401 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
     8461 gtcttattac cgcagatgca agcttttgcc acacctcttt cttgtcattt acagactcaa
     8521 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
     8581 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
     8641 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataat
     8701 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
     8761 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
     8821 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
     8881 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
     8941 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
     9001 attttgcata attgaaggca gccaattcat taacccaaaa tagccggcaa tttggacagt
     9061 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
     9121 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
     9181 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
     9241 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
     9301 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
     9361 caaggacata cacgccccgt attctcctcc cgctcctaca cctgcgataa agcgaaagag
     9421 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
     9481 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
     9541 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
     9601 aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa tatccatatt
     9661 ctctaaagca attcccgccg atgtcgataa taataacttt cgctgatacg ttgtcatttt
     9721 tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa aagaataaaa
     9781 aaacaagact tttttatttg aaataataca caaacaaccg atatagataa tccagcttag
     9841 ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata aataaaatac
     9901 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
     9961 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
    10021 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
    10081 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
    10141 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
    10201 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
    10261 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
    10321 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
    10381 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
    10441 caactgaagg agttagaaga ggaactagga acagaactgt ttgtacgcga aaaccgcaaa
    10501 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
    10561 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
    10621 tacgatcggc tgtgtggaag ctgataattc gaatacattg gcactattac tagaaaagtt
    10681 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
    10741 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
    10801 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
    10861 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
    10921 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
    10981 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
    11041 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catctttcaa agcgagtgac
    11101 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
    11161 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
    11221 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac agtcttgctt
    11281 ttgtattttt tcatttttaa atcaaaaaaa ataaagagat ttacataact tgttaaataa
    11341 gcgcttacaa acataagaag aacggaaatg aatcagctat aataaccgtt gttctggatt
    11401 gtaatcatta caaagaaatg aagattataa ctttggaggt gttcgaaatg aaagaaaaaa
    11461 agcaaaaatt cgttcaaaaa acatgccctt tattcaatgg ttgggtatga ttctgctgat
    11521 tgtattagct ttattgacga tcattccttt tttatggatg gtttctgctt cattcaaagc
    11581 aaataatgaa gtgttcacga ttccgattca atggatccct aaaacttggc atttagagaa
    11641 ttatctggtt atttggtctc gtattccttt agtgacattt tttaagaata cagctttttt
    11701 aagcgttgtg atcacgttga ttcagttgtt tacttctagt tttgctgctt acgggttttc
    11761 taaactaaat tttaaagggc gcgatatttt atttttagca tatatcggca cgattgctgt
    11821 cccttggcag tcttatatga tcccgcagtt cattatgatg agaaaagtgg gtttaacata
    11881 tacgttgtgg tcgttggtgc tattacaagc gttcaatgct tttggtgtgt tcttgttgaa
    11941 gcaatactac agtagtatcc cagattcttt atgtgagagt gcgcggatcg atggcttatc
    12001 agagtgggga atctatcgca agatcatttt acctcttaca aaaccggcga ttgccagttt
    12061 gacgatcatc acattcgtta atacatggaa cgattatatg gggccattca tttatttgtc
    12121 ttctacggaa aataaaacaa tccagttagg gttgaaaatg tttattgggc tatttgatgc
    12181 agagtacgca ttaatcatgg ctgcctcagt tgtttctgtt cttcctgttg cttttatatt
    12241 tttagcgatg caaaaatatt ttgtagaagg gatcgcttca tcaggaatga aggaatagga
    12301 ggtgattatg aaagagcagc gttttgacaa aaatgtttat atggaattct tttattggtt
    12361 gtatcaatca ttggcaagta gcctttgttt ttggtgtgtc aatatcttgt atatagcagc
    12421 tgcgttcctt ttgtcgatag atggaaggaa tgtcttttcc tttatttttt ctttactttt
    12481 tattggtcca ggttgcatcg tgatgatagg tatgacagaa gaattagcaa aagaaaaaag
    12541 tttttcacca gtacgtttgt tcataaagac gtttcgccaa ttttggctaa agggattttt
    12601 ttattggcta tttgcttgga tagttagtgt gataatgatt tttgattgtt tcttttttat
    12661 acgtttttct tatggaaaat ggttgattcc gttgtttgtt ttacttgctt gtttatcagt
    12721 ttcctttagc atcaactact ggtattttca ggtgagaaat ccagcgtcga aaccaaatca
    12781 agttctacgg atagcgttct actatacgtt gaaaaaatgg tatgtgagtt tactagattt
    12841 tttactattg acaagtttgt ttctcttctt tttcgtaaag ccccaatggt gtattctact
    12901 aggtccttct atcgtatttg gtttgattta cttcaacaat cgtaaattga tgagaactat
    12961 ggatctgtag actttataaa ttgaaacttt caattaagaa tgacagtttt tatgtaaaaa
    13021 ctgtcattct ttttcatttt ttgaaacata tggctaaaca aatgaaataa aacgtgctat
    13081 acttttgaat gaaacaggtt tagaaactgt atcaattatt tgtgattatt tctaactaat
    13141 aattaaaaat aaagcataaa aaggacgatg ttcaatggac gaaaaaatga aacaaggttc
    13201 gaaatctgta gtagaaagtt tgattaatca tcatgttgat tatgtattcg gtataccagg
    13261 tgcaaaaatc gatggtgtat ttaatgaatt agaggatcaa ggtcctgaat taatcgtgac
    13321 tcgtcacgaa caaaatgctg catttatggc acaagcaatc ggacgtatta caggagagcc
    13381 aggagtagtt atcgcaacaa gtggtcctgg tgctagtaac ttagctacag gtttggtaac
    13441 agcgacagca gaaggtgacc cagttttagc aattgccgga caagtaaaac gaagcgatct
    13501 attgaagttg acccatcaaa gcatggataa tgcagcactg ttccagccaa tcacaaaata
    13561 cagtgcagaa atccaagatc cagaaacaat ttcagaagtg attgccaatg cttatcgtat
    13621 ggctaagagt tcaaaaaaag gcgcgagctt tatcagtatc ccgcaagatg tagtggatgc
    13681 acctgtccaa gggaatgtca tcaagccatt gagcgatcct aaattaggtt ctgcttctgc
    13741 agatgatatc cgttacctag ctgaacgcat tagagaagca aaactaccgg tactattagt
    13801 aggtatgcga ggatcaagtg aaaaagaaac gttagccatc aggcaattag ttggaaagac
    13861 agcattgcct gttgtagaga cattccaagc agctggagtg atctcacgtg aattagaagc
    13921 gcatttcttt ggtcgtgtgg gtttatttag aaaccaacca ggagatatgc ttttgaaacg
    13981 aagtgatcta gttattgcta ttggctatga tccaatcgaa tatgaagcaa gaaactggaa
    14041 tgcggaaaaa gatgcgcgga tcattgtgat cgatgaagcg ccagctgaaa tcgatccttt
    14101 tatgcagcct gaaagagaat tgatcggtga tatctcagcc actcttgatt tattgacagg
    14161 ttcattagag cctcaacaag tgtcagaaga tgcaaaagaa tatttagcat ctcttcaagc
    14221 agaattaacg gagcgggaca ttgttcaatc aaaaggagaa gcaggtatct tgcatccttt
    14281 agaagttatt aacacgcttc agtcaaaagt aacagatgat atgactgtta ccgtagatgt
    14341 aggtagtcat tatatttgga tggcacgcca ctttagaagt tatgaacctc gtcatttgtt
    14401 attcagtaat ggtatgcaaa cgcttggtgt tgcattgcca tgggcgattt ctgctgcatt
    14461 ggtgcgtcca aatacacaaa tcgtatcagt ttccggcgat gggggatttt tattctctgc
    14521 ccaggattta gaaacggcgg tacgtaaaaa attgaatatc gttcatttga tttggaatga
    14581 tggacactac aatatggtag agttccaaga aaaaatgaaa tatcagcggg cgtcaggtgt
    14641 agactttggt ccagttgatt ttgtgaaata tgcagaagct tttggcgcta aaggaatccg
    14701 agctacaagt gtagaggaat tagaaaaagc tttagaagaa ggatttgcga cagaaggacc
    14761 cgtaattatt gatattccga ttgattaccg tgataatgag aaattgggag aaacaatctt
    14821 accagatcaa ttctattaag gaagtgtcga catgactgaa aaaatcttat accagcacgg
    14881 gacattagga gctttgatgg ctggcttgat ggatggaaca gaaacgattg ctcatatatt
    14941 agaaaaagga actctgggat tgggaacatt gcatgggctt gatggtgaag tcatcttttt
    15001 agatggcgta gcttatcaag gaagatcaga tggctcagtt gcccaattag atggaagtga
    15061 acttaccccc tatgcagcaa ttacagattt tacgccagat actagttttt ctgtctcaaa
    15121 aactgcagat ggagaacaat tgaaaaaaga catattagta agagaagccg gggaaaacct
    15181 atttttggca gtgaagataa ctggattatt caaaaatatg cacatccgta tcatgccgaa
    15241 gcaagaaaaa ccttacagaa gactagcaaa aatttcagag tctcaaccag aattccaaca
    15301 aagcaatgtc caaggaacat tagtcgggtt ctttacgcct gaattattcc aaggagttgc
    15361 tgcagcggga tttcacttgc attttatcga tgatacacat acatttggcg gacatgtaat
    15421 ggattttgaa gtggctgaag gaaaagttga gattagccgc atctcgtccc ttgtacagca
    15481 ttttccagtt gatgatccga cttttgttca cgaaacgatt gattatgctg atttagcagc
    15541 agaaatccaa caagcagaat agaaaaagac agcgagatgc gttcaaggtg attaacctga
    15601 cgtatctcgt ttttttgagg aggaaaccta gcgtttacaa aaaaactgag ctataatctt
    15661 ctccatagat caaaaaggag cggtttttat catgtggaca gaaaaacaga aaaaagatgc
    15721 atacgaaatt ttattattac agcaacaagg attattagaa gcagaagata actggttagc
    15781 aaatttagca aattcatctg cattgttgaa tgagactttg cctgagactg tctttgcagg
    15841 atattattta ttcgatggta atgaattgat tttaggaccg ttccaagggc gagtttcttg
    15901 cacacgtatc aagatgggaa aaggagtttg tggagaatca gctgaaaaac aagaaacatt
    15961 gatcgttgat gatgtcaaaa cccatgcaaa ttacatttcc tgcgattcag ccgcacaatc
    16021 agaaatcgta gtgccaatgg taaaagaagg taatttaatc ggtgttttag atatcgattg
    16081 cggagtaacg aaagggtatg atgaaatcga tcaagcattt ctagaaaaat atgtagatct
    16141 tttattagca aattttaata taaatgcttt ttcatttatt aataactaaa atcgtctatt
    16201 gaaacaaaaa agaagagaaa tcaagaataa aaagagccgg aaagacagtg ggcatttctg
    16261 tttttcggtt tttttatttt tttataaaat cacaagtgtt attgattttc ataatcatat
    16321 tttaatggta ttatcatttt aaataatgga aaaacattaa ggatggatga aatatgaata
    16381 acgaaactat cattttagga aaaaaatata tttgccaacc tattggcttg aaacaaaaag
    16441 tggttggaga agtaatcagt aaattagaaa attgtgttgt gatctgcgta gacgaatatc
    16501 atgacgtcga tcgcaatgaa atattagaaa aatgcggaaa agtagtggca aaatatactc
    16561 acgtttatga gttggaaaca acagaatatc tatataacta tagcaacaaa tatgaacata
    16621 ctttagtttc ataaagattt aaataaagtg ccttcaaaag cgagaagacc aagcttttga
    16681 aggcacttta tttagttggc agtgattgtt catgattttt gatttattca aaaggttcgc
    16741 ctagagaatc tgtctctgta acgaggcctg gtggagaaac tcgataaaaa cctatactgc
    16801 cagaactgtc ctattttcgg atagacaaac tagtagtttt aaaaagatca tccgatccaa
    16861 tcatttcaaa aaatgcaaaa ctgatatcag gatcatttag ctcaggcttg ttatcgtaaa
    16921 gcatttgtaa aggttgcatt tagttaggag taaacgcgaa actatcatca ttatttgctg
    16981 aagaagatga agaagctaat tcagagtttg tttgaaataa aggagacgtg ctgacagttt
    17041 ctttaccctg ttttttgcag aggtagaaac ttttatatcg gaagtttcta ccggagaatt
    17101 tttctcattt gtagaaattg ttgaatcgat actattcgaa atttgcgtat ctgaaggtat
    17161 gttctttgat gcagaagtcc cacaagcagt aagtaacaat aaatttgata aactgataaa
    17221 cagaattctt ttcattatgt acacactcct tatagtatcg tttagcatac acctatgctc
    17281 agtattaggc aaaatcaatg caaaactatc aagtctttta tagtatctat tagtcttagt
    17341 aatacatata tggagagggt gatatcccag ttcaaaccag atacattggt taccatctgt
    17401 attaacgatt atgcctctaa acgctttttt tatgaaaaaa tttataagtg aaaacgtagg
    17461 tatgatatta tctagaggta atttttttgg gtatttaaat agttgctagg aggatag
//
LOCUS       DACEUF010000053        17184 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.056, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000053 DACEUF010000000
VERSION     DACEUF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.056"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(543..2048)
                     /locus_tag="IXG20_001773"
     CDS             complement(543..2048)
                     /locus_tag="IXG20_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1609969.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IXG20_001774"
     CDS             complement(2045..2731)
                     /locus_tag="IXG20_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1609970.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IXG20_001775"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IXG20_001775"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ1609971.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IXG20_001776"
     CDS             complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IXG20_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1609972.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4752..5321)
                     /locus_tag="IXG20_001777"
     CDS             complement(4752..5321)
                     /locus_tag="IXG20_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ1609973.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5507..6409
                     /locus_tag="IXG20_001778"
     CDS             5507..6409
                     /locus_tag="IXG20_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1609974.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IXG20_001779"
     CDS             complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IXG20_001779"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ1609975.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IXG20_001780"
     CDS             complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IXG20_001780"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ1609976.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IXG20_001781"
     CDS             complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IXG20_001781"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ1609977.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12977..13162
                     /locus_tag="IXG20_001782"
     CDS             12977..13162
                     /locus_tag="IXG20_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ1609978.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(13224..14420)
                     /locus_tag="IXG20_001783"
     CDS             complement(13224..14420)
                     /locus_tag="IXG20_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1609979.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14590..16026)
                     /locus_tag="IXG20_001784"
     CDS             complement(14590..16026)
                     /locus_tag="IXG20_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1609980.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16319..17128
                     /locus_tag="IXG20_001785"
     CDS             16319..17128
                     /locus_tag="IXG20_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1609981.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         5042 a         3595 c         2983 g         5564 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttttatt
     4441 taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt tttacatttt
     4501 ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata gtaaccacaa
     4561 tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc gtttaaccct
     4621 ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga tgttcttcta
     4681 gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat tagacacaat
     4741 atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag ttcagatagt
     4801 tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg ctggtattct
     4861 tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc ttcctcttct
     4921 tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga ctctgctaaa
     4981 ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc atctctactt
     5041 tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg cagttcaaca
     5101 ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta atgaacgata
     5161 tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac atcaagtatt
     5221 aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa agttaacggt
     5281 gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca ctcctaagca
     5341 acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt gacaccttgc
     5401 agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat catcctcaga
     5461 atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga acacgaaaga
     5521 tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg ctaattattt
     5581 ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag agtttgacac
     5641 cgtacttatt acaagagatc gatcgcatca cgaattgatc gtcacgcctt ctggaaagca
     5701 gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag ctgaaatcac
     5761 tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca atgaatactt
     5821 tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg agattaccga
     5881 tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt tagctgtatt
     5941 aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt tggacaaacg
     6001 tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg tctctttcaa
     6061 agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc ccgcagcatt
     6121 cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa gcaatgactt
     6181 gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg cagagattgc
     6241 tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc agcctcgctt
     6301 cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag aacaagtagt
     6361 ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa agaagagctg
     6421 tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta aacgatgccg
     6481 cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc gcctttagca
     6541 ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga agtcaatccg
     6601 ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc tttgtcagtt
     6661 gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc agcagatgaa
     6721 gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc taataattta
     6781 gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac tggtacacca
     6841 gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc ttttttagct
     6901 gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg gtaaacgccc
     6961 cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa gatatctgca
     7021 tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc agcaacgatt
     7081 gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt ttcagttaca
     7141 tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc attttcagct
     7201 tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc gtttgcagat
     7261 tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac gtcagaagct
     7321 tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt agcagtgata
     7381 actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat tggtactagt
     7441 tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc agaaactttg
     7501 atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa gatttgaaca
     7561 tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg tgctttacgt
     7621 acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat atcgtcagca
     7681 tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac ccctttacgt
     7741 gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc ttcgtctttt
     7801 tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc aacgtgaaca
     7861 tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac atgttcagga
     7921 tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc acgaccaaaa
     7981 tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt atctaaaaga
     8041 ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat acgtgataat
     8101 tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc agattcaatc
     8161 aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac gattttggtt
     8221 tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta cacagcttaa
     8281 atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat atagtgataa
     8341 gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa cgatttcgtt
     8401 gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga cagcgtaagc
     8461 accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac ggacaacgtg
     8521 tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct cagcgaattc
     8581 atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc cgcgatcgcg
     8641 gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg ggatgatgat
     8701 ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat tgcgtcccat
     8761 tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac ggtcaattga
     8821 ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc caggaatatc
     8881 attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca aagccattgc
     8941 gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga attttttcaa
     9001 ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac gtgcagaata
     9061 caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa gacgacgaat
     9121 atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca gtccttcgta
     9181 aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt ctccgccact
     9241 ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac tttatcctaa
     9301 gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct aacattattt
     9361 ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca agtagtattt
     9421 tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat aagttgccgc
     9481 tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt tcgttgcgtt
     9541 ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat cacttgtaat
     9601 tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca agtttgtgaa
     9661 ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag ttgcagttcc
     9721 tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc aacggcaaac
     9781 aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc tccactactg
     9841 tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc tctaacttcc
     9901 ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat cagctgaact
     9961 tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa ataaatacct
    10021 aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac ttcttcttct
    10081 tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat attttggatt
    10141 tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc aaaaactcct
    10201 actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat ccgtatgagg
    10261 aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat atcttctaca
    10321 aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc cttattgacc
    10381 aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag tttattttta
    10441 cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga attcatcagg
    10501 ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa tcccacgaat
    10561 gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc gatatattca
    10621 tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga tacaaaatgc
    10681 gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg cagaatatct
    10741 gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg ttcctgatag
    10801 acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg gtagcgaatg
    10861 gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg gcctggtcga
    10921 tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct tcgtaaaacg
    10981 ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg ttgaaacaag
    11041 agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata cccttctact
    11101 cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc taaaaagtcc
    11161 attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag aaaagcttca
    11221 tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat aactacacct
    11281 gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt tttgaataaa
    11341 agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt tttataagct
    11401 tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt tgcttcactt
    11461 tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc cattgtgcca
    11521 aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata attcaatact
    11581 tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa gcgttcgggg
    11641 tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt gatttctaac
    11701 acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac gattttgttt
    11761 ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa tcccatttga
    11821 tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata gtcagtgatt
    11881 cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg ttttccatta
    11941 ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga aatcgattca
    12001 gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa tcgtttgatc
    12061 atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc agataaattt
    12121 tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc aaaaagttct
    12181 tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc ttttatcaca
    12241 tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat ataaaaattt
    12301 ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc ctctttattg
    12361 aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc ggtttctgtc
    12421 atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt catcagctgc
    12481 tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc ttcataagta
    12541 taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat acgatagaat
    12601 tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta gccttgtttt
    12661 tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga ataagaagta
    12721 attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt taaaattcac
    12781 tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg aagttgatta
    12841 ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa gaactcaaat
    12901 tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa attaagatag
    12961 aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg ttctaggtca
    13021 agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta ctttgacaac
    13081 aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg cgccaagaaa
    13141 agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc aagctctttt
    13201 ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg aaagttgaat
    13261 aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac agcactgtaa
    13321 ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc aaagttgcta
    13381 aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat cttactgatc
    13441 agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa gcgaagtata
    13501 cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa tgataaaatg
    13561 agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc tagtttcggt
    13621 gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac aaaaaggata
    13681 ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct gatcgtagtg
    13741 attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga gagatgatcc
    13801 atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg atggaaatct
    13861 tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc tgtgatcaag
    13921 aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg tccgatcaga
    13981 tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt ttctctaggt
    14041 gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat cccattccaa
    14101 aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc cattgttaat
    14161 gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc agctagattt
    14221 ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc tagtcccgaa
    14281 aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg tatgaatggc
    14341 ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat cactaaattt
    14401 tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat acctattttc
    14461 gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag gtcattttat
    14521 atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt tccaatccct
    14581 tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa tatcaacgat
    14641 ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa ttgtatagaa
    14701 aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa agatcgatgc
    14761 aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa aagaagccat
    14821 catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta caatggcgat
    14881 cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg aagtcaaacc
    14941 agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg tattcgatgt
    15001 accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt tatccatttt
    15061 tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga aaatacctgt
    15121 gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata aactgaatgc
    15181 gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag attgcatgcc
    15241 ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat tacctaacga
    15301 attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac caaggactgt
    15361 ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta aaatggctgt
    15421 cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg ctaacgcagg
    15481 gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga cagagctcga
    15541 aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc cgacatacgc
    15601 aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa tcgcccgaat
    15661 aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc aaataaacac
    15721 cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa aagtgaaaaa
    15781 tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca atcccattac
    15841 cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga agggcatacc
    15901 tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa agaatgtcgt
    15961 tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac cgaaaaactt
    16021 ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa taataaaaaa
    16081 taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt tcattcgctt
    16141 ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt ttagcttatg
    16201 tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat tgctcttgtt
    16261 aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag gacccttatt
    16321 gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag attcgatgat
    16381 tacatctaaa acaaaagaga ctctgaaaaa agcaatcaat gccggtcatc atgtcagtat
    16441 tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag aattaacaac
    16501 accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact gggatggtga
    16561 aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa aaaagcagct
    16621 aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca gttttgattt
    16681 ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc tttctcctaa
    16741 aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt ttgcaggagc
    16801 tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga catggggtgg
    16861 acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag gcgtgcaaga
    16921 gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg acgagcataa
    16981 tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg gtactgacca
    17041 actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg gtttagctgt
    17101 ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa aagaggttgt
    17161 gacaattttt gtcacaacct tttt
//
LOCUS       DACEUF010000054        17082 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.057, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000054 DACEUF010000000
VERSION     DACEUF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.057"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(17..538)
                     /locus_tag="IXG20_001786"
     CDS             complement(17..538)
                     /locus_tag="IXG20_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1609982.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..4429
                     /gene="nifJ"
                     /locus_tag="IXG20_001787"
     CDS             737..4429
                     /gene="nifJ"
                     /locus_tag="IXG20_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ1609983.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4618..5505
                     /locus_tag="IXG20_001788"
     CDS             4618..5505
                     /locus_tag="IXG20_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ1609984.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5502..6671
                     /locus_tag="IXG20_001789"
     CDS             5502..6671
                     /locus_tag="IXG20_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609985.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6695..8050
                     /locus_tag="IXG20_001790"
     CDS             6695..8050
                     /locus_tag="IXG20_001790"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ1609986.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="IXG20_001791"
     CDS             complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="IXG20_001791"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ1609987.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10336..10626
                     /locus_tag="IXG20_001792"
     CDS             10336..10626
                     /locus_tag="IXG20_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609988.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10639..11664
                     /gene="sppA"
                     /locus_tag="IXG20_001793"
                     /pseudo
     CDS             10639..11664
                     /gene="sppA"
                     /locus_tag="IXG20_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
     gene            11667..12326
                     /locus_tag="IXG20_001794"
     CDS             11667..12326
                     /locus_tag="IXG20_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1609989.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12483..13415
                     /locus_tag="IXG20_001795"
     CDS             12483..13415
                     /locus_tag="IXG20_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1609990.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13569..14858
                     /locus_tag="IXG20_001796"
     CDS             13569..14858
                     /locus_tag="IXG20_001796"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ1609991.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15105..16355
                     /gene="clpX"
                     /locus_tag="IXG20_001797"
     CDS             15105..16355
                     /gene="clpX"
                     /locus_tag="IXG20_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ1609992.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16438..17025
                     /locus_tag="IXG20_001798"
     CDS             16438..17025
                     /locus_tag="IXG20_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ1609993.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         5586 a         3066 c         3740 g         4690 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata
     4441 taatgaataa ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact
     4501 tgcaattttc ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcata tcagcctcta
     4561 aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg
     4621 tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac
     4681 ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta
     4741 tacaaactga tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct
     4801 atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg
     4861 atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc
     4921 cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag
     4981 caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga
     5041 aaaatcggtg cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca
     5101 gggattgctt tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat
     5161 acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc
     5221 tatcttcctc tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca
     5281 gctgtcggta taagcgaagt cagtgacgcg attaccatcg ttgtctctga agaaaccggg
     5341 gacgtcagca tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg
     5401 gcaatcttgc gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt
     5461 caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac
     5521 ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg
     5581 caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta
     5641 cgggggtgcc gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa
     5701 atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg
     5761 aagatacacg gatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg
     5821 aagttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga
     5881 ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc
     5941 cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag
     6001 tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg
     6061 acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg
     6121 cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg agagctgatt
     6181 cgatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg
     6241 gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg
     6301 atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc
     6361 atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat
     6421 cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga
     6481 gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa
     6541 cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca
     6601 caagtacaga agaaacatca gaaacaagcg aatcaacaca aatgagcaga acaaattcct
     6661 cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg
     6721 cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg
     6781 cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg
     6841 gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc
     6901 ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac
     6961 acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga
     7021 taatggaatc aaatttttcg gtgcagatgg ttttaaacta gtagatgacc aagagctaga
     7081 aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt
     7141 gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac
     7201 gatcaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc
     7261 aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa
     7321 tccagacggg ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga
     7381 gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat
     7441 tattgcggtg gatgaacttg gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc
     7501 taaatacttg gcagaaaaaa atcgattgaa aaaagatacg atcgttacga ctgtcatgag
     7561 taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt
     7621 tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca
     7681 gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat
     7741 tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt
     7801 aacaatctat ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga
     7861 tgttccggca atcaaaaaag tgattgaaga ggcagaagct gaaatgggat cagaaggaag
     7921 aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac
     7981 tgatgagaaa gttaattact atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg
     8041 actggattaa gagatatttt cataaaaaat aaaagaacaa ggaccagtgt cagatgtctg
     8101 acactggtcc ttgttctttt attattctac tgtcactgat ttcgctaaat tacgtggttt
     8161 gtcaacgtcg aatccccgta atagcgttgc gtaatatgca agtaattgag tcggtacgac
     8221 tgtggttaga gtagttagat aagggtggac agttggtagc accagctggt cactttcctt
     8281 ttctaatccc tctgagacaa taactagtgt tttagcgcca cggctttcga cttctttcag
     8341 gtttcctcga gtatgcgcac cagtcacttc ttcactgatg attccgatca ctggtgttcc
     8401 ttcttcgatc aaagcaatcg ttccatgttt caattcgcca gcggcaaaac cttctgcctg
     8461 gatataactg atttctttca atttcaaagc agcttcaaga gaaacgttgt agtcagcacc
     8521 acgtccaatg taaaaggcat ttcttgtatc gctgagatat acttctgcca actcttctaa
     8581 ataatctttt tgggcgatga ccgattccat cgcattagca actaaactca attcatgagt
     8641 gatatcgaat acaagagaag tttcaaattc ttttttatca ccgatagctt tggctaataa
     8701 agttagtaca gctatttgtg cagtataagc tttcgttgat gctactgcga tttcgggtcc
     8761 agcatgcaat agcagtgtaa aggatgcttc ccgcgaaaga gtagaaccag caacatttgt
     8821 aatcgtaagt gaagggtagc cgagacggtt gatttttacc agcacttggc ggctgtctgc
     8881 tgtttcgcca ctttgactca agaaaataaa gaatggtttt tttgaaagaa gaggtgtgtt
     8941 atagccaaat tcactggcta aatgaacctc aacaggaatc tgtgtcaatt tttccaaagt
     9001 ctgtttacct gctaaccccg catggtaact agttccgcaa gcaacgatat agattcgatc
     9061 gctatcagac aatgtatcga ccagcttttg atcaagagag acacgattgt cttctgttac
     9121 gtatttttga gcgatacgac gcatgataat aggctgttcg tcgatttctt tcagcatata
     9181 aaaaggatag gtacctttct cgatatctga agcatctact tgtgcttcga atgaagaacg
     9241 ttctacgatg tcacctgcaa aattttgaat aataactttt tctgatgtga cggtaaccat
     9301 ctcttcatct tcaatctcaa cgaattcctt cgtatgagca agcatagcca ttgcatcgct
     9361 ggcaatcaca ttgaatccat cgccgagtcc gattaatagt ggacttttat ttttagcgac
     9421 gtaaataaca tcaggtgctg tccgatcaat caaagcgaaa gcgtaagaac ctttgatgac
     9481 ccgcaaagct tttaaaaacg cctcttgcgt tgtagtatcc ttcgcgaaag tttcaattag
     9541 atgagcgata atctcagtat cggtctcacc aataaagtgg tcatttgcta aataagcaat
     9601 tttaagttct tcaaagtttt cgattacgcc gttgtgaacg agcacgaagc gtccactttg
     9661 ggaagtatgt ggatgggcgt tttcttcaga aggttgcccg tgagttgccc atcgtgtatg
     9721 accaatacct atatttcctg tagtatcaat atttattttg tttttcaaat tttgaattct
     9781 gccttgtgct ttgaccagat aatcagttgt gccatctgag acaaagatac cagctgaatc
     9841 ataaccacgg tattctaatt tttctagacc gtcaatcaat cctggtgtta cattttttaa
     9901 tccaatcatt ccaacaattc cgcacataaa aaacatccta ctttctataa tgaattcgta
     9961 tgaatgcttc cagctgcagg aaaatccttt tgttttatct ttgcagacat tttttgtgga
    10021 ttttctgtag agtgcagtcc ctagggccat ccgccgaatc tttcgataaa ccctctcctc
    10081 gtcatctgtt tacacagacc tggcgctatc ctaaaaggta taactatcct cctttttgtt
    10141 ttttatctgg aaacatttaa aatcataacc ttttgcgaat gttcttgtca acaaaaaaag
    10201 aataaaaaat aaattggtat atatcaaatt ctttgtcaaa tgtccttttt atgtcaaaaa
    10261 ataatgggtt tttatctatc atctttttca ggagttcggt acaatagaaa gtaatcggat
    10321 agaggaggag taaaaatgga ttcaaaaatt gagatcatga cactgggaat gttaaaaaaa
    10381 caattgtcca aattcgaagc atcagctggt gtatcagatg atacgaaaat atttttagat
    10441 actggctggg atagcatcca agaaatcgca cctgatgcct tggaagttgc tcaagctcgt
    10501 gagtttacgg tagaagatga attaacaaaa gagtcgtttt ccgggtatgc gcgggaagaa
    10561 aaagcagagc ggttcgacac ttctgagcag tcagaaactg tgatcgtcat aaaaaacttg
    10621 tattagaaag gagaaagaat gaataaaaga cgttgggcag cagttctgat tgctgcagga
    10681 ttatttgtgg tttcgttagt ttcagctagt ttgactaagc ctcaggaaaa cgatcaaatt
    10741 gagggattga acagttggtt atatggggat gaagaactag ctccaaatgt gttggaagag
    10801 ggagacagta cgcaaaaaat cgtataatta tcagtagatg gaacgattgc tgcgggaggg
    10861 tcttcaggtt tatttgcttc tgaaggttac aatcataaaa attttatgga acaactgaag
    10921 gcgattgaag aagatccaac agtcaaaggt atctttttag aagtaaattc tcctggtggc
    10981 ggtgtctacg aaagtgcgga aatcgctaag aaattggata cgattcgtaa agaacatgat
    11041 atcccaatgt atgtcagcat gaaaaacatg gcagctagcg gtggctacta tatttctgct
    11101 caagcagata aaatctttgc aacagaagaa acagtgaccg gctcaattgg tgtgatcatg
    11161 tcaggattga attattcagg acttctagaa aaactgggtg tggaagatac aacagttaaa
    11221 agcggagcgt tgaaagatat gggttcagga acgcgaccag agacaaaaga agaagaagcg
    11281 gtgcttcaag catatatcga cagcgcatat agtcgttttg tagcaatcgt cagcgaggga
    11341 cggaataaat ctgaagaaga agtcaaaaaa attgctgatg gaaggattta tgatggtgtc
    11401 caagcaaaag aagtaggttt agtcgatgaa ttgggatttc ctgaagatgc tttaaaagct
    11461 atacgaaagg aacaacagct tgaagatgcg gaactagtag aatattcttc cacgccatct
    11521 gggtttgcta gtacatggtt aggaagtaaa ctagcagaat tccagggatt gaagccttct
    11581 gagactagtc agatcttatc cgttcttgag aatctaggga cagcggaatc accaaaagca
    11641 atgtattatt acggaggtga gtaagcatgt cagagctttc aaatgaaaat aaggaacaag
    11701 aaaaagataa gcaacagcag cctgctgtag gacctgactc caacttttcc caaaaggtac
    11761 tgagctcttt tgaaagagag cctttgacgc ccgaagaaat caaaaagaaa caacgtaaat
    11821 ggaaaaagta tacggacaca agaaatgaac cgccaaaagc aaatgatttt cctaagtatt
    11881 tctatgcagg tttttttatc cgaatgtttg ccttcttggt cgatcttttg tgtatcggag
    11941 ctattacgcg tttaacactt ggtattgcca aaaatctagg ttggcttaat ttttcagatt
    12001 cttatctgag tctctacgga ttcacagcct tacttattta tctagcgtat tttattttat
    12061 tgacgaaatt caatcatggc caaacgattg ggaaaatgat ctttggtatt cgagtcatca
    12121 gctttgatga agaagaactt agctggtcaa ccgttttagt tagagaaggt gcttgccggt
    12181 tcattctaaa atttcctttg ctgatgttag ggtatcttcc agcagtcttt aatcagaaaa
    12241 agcaacatct gggcgatttt ttcactaata caagtgttgt tacgatcaat ctgatcaaag
    12301 cgttcaacca ggaagtcaac agctgatgag caataaaaag cagtaacagt tgaaagcacc
    12361 ttctaaaagt cagatctaaa atctagcttt tagagggtgc ttcagttatt tttaattgtc
    12421 atgctttttt tgttttgcta ctattgaaca tgagctgaat atgagcaaga ggaggacact
    12481 gcgtgaagcc gattgatttt ccagaaaaat atgagaattt aatgcgagtg gcacaacaag
    12541 cactagcaaa ccagcaatat cagcaggcaa aagaattatt tcaacgagct tacgagttga
    12601 aagaaagctt tgaggctaat agcctgcttg ttttttgtct ttacgagttg gacgagaaaa
    12661 aagaagcatt gaagcaagca ctcttgcatg aaaaacagta tcttcaaaat gaggagtttg
    12721 ctgaatttta ttttgacttg ttaatcagta cacaagattt tttatatgct agaaaattga
    12781 tagcaagtac agatttttat gaagctttcg agcagcgggt cattgaaaaa atccaatttg
    12841 cagaagaatt atccggacaa atggagaggc agaagatcaa ggcactccac aataaatcgg
    12901 aggaacttcc ttcgttggaa cctgcacgtc agcttgcgtc tattgatgag atcgaacagc
    12961 ttccttatca tgagtttatc caaacagcga gtaaacttat tgtatcgcca gaggtccata
    13021 ttttagcaag ggcaaaatta ctggagacac ttaggcaatt gaacgaatgc catcctgttt
    13081 tctatttgac gattgaagaa aagttagtca aagtcatacc aaaagatctg ccaaagccgc
    13141 agcagcaggc aagttaccag caattatgtg tattttcaga tcgttacggg aatgaagatg
    13201 cgctgcttag ttccgtgtta aaagaagaat tcacgttgca atctgcaatt gtttatcctg
    13261 tctatgatac atacatcgaa gacccgaagc gctggtttca actgactgta gaggcgtgta
    13321 caggaaaaac gatgtatgaa ggtacagaag atgaaaaaca agatttttta aaaaaaagag
    13381 aaaaaatttt gcagcaaatg atcttcttcc attagatttt atcagtgaaa aagataaaaa
    13441 tgttatgaaa aacaaggatt ctatccattc aatatgttta caatttgaaa gggttagtgt
    13501 actatttaaa ggtatgcatg aaacgttttt gatgtataaa atgaatttct aaactcggag
    13561 ggaattaaat gactgcaaca tttgaaaaaa aaggcacgaa tgatggtgtt ttaaaattta
    13621 cgattgcgca agacgaaatc caaaaaggat taacaactgc gtttaataaa gtgaaaaaaa
    13681 gcttgaacgt acctggattc cgtaaaggga aagtttctcg tcaagtattc aaccgtatgt
    13741 acggtgaaga agctttatat gaagatgcat tgaatgctgt attaccagaa gcttatgaag
    13801 ctgctgtcaa agaagctggc atcgatcctg tttctcaacc aaaaatcgat gttgaaagca
    13861 tggaaaaagg tcaagactgg gtaatcactg ctgaagtaac agtgaaacca gaagtaaaat
    13921 taggacaata taaagatcta gttgttgaaa aacaagaccg tgaagtaaca gatgctgacg
    13981 tggatgcacg tatcaaacgt gaacaagaag cacaagctga attagttatc aaagaagatg
    14041 ctgcagctga aaatggcgat acagtagtaa tcgacttcga aggctttgta gatggcgaag
    14101 cgtttgatgg cggaaaaggc gaaaattact cacttgaatt aggttcaggc tcattcattc
    14161 caggttttga agatcaatta gttggtcata aatcaggtga aagtctagat gtaaatgtaa
    14221 ctttcccaga agattatcaa gcagaagatc ttgctggaaa agaagcagtc ttcaaagtga
    14281 caatccatga agtaaaagca aaagaagtgc ctgaattaga tgacgaattc gcaaaagatg
    14341 ttgatgacga agtatcaaca ttagacgaat tgaaagaaaa atatcgcaaa gaattagtag
    14401 aaacaaaaga aaaagcagcg gacgatgcaa aagatgaagc agcgatccgt atggctgttg
    14461 aaaacgcaga aatcgttgaa ttgccacacg tgatggttca tgatgaagta catcgttcaa
    14521 tggacgaatt cttgaacaac atgcaacgtc aaggtatctc tcctgaaatg tactatcaat
    14581 taactggttc tacagaagaa gacttgcata aacaattcga aggtgaagca gaaatgcgca
    14641 caagaacaaa tcttgtgatc gaagcaattg cagctgctga aaatcttcaa gcaacagaag
    14701 aagaaatcaa tgcagaaatc caagaattat cagaaacata caacatgcca atcgaacaaa
    14761 tcaaacgcgt gttgacagaa gatatgctgg aacatgatgt gacaatgaaa aaagcaatcg
    14821 aattgatcac aggtacagcg acagaagcga aagaatagaa atcaagtaat aaatcatttc
    14881 tgaagaagct acttctagaa aattgtttat tcgaaataag ggactgtgac acgattttgt
    14941 cacagttcct tattttttgt taagagtaaa aaatgcaaag gtataggtca gttgatcctt
    15001 tgttaaacac tttttctttt tactaaacta tgttatgata gtaaaagact gtaaagtctt
    15061 tgatagtttc ttgcatactt tttgcaagtt aaggggtgaa gaccatgtat gacaatccaa
    15121 gtagcaatga gaccgtacgc tgttcttttt gcgggaaaac ccaagaagaa gtgaaaaaga
    15181 tcgtagctgg tccaggggtc tacatctgta atgaatgtat cgacctatgt aaagaaatca
    15241 ttgatgaaga attttatgaa gaagctgtac gcgaattcac tgaagtccct aaaccactag
    15301 aaattcttga agtattgaat aactacgtga ttggacaaga tcgcgcaaaa cgctcgttag
    15361 ctgttgccgt atacaatcac tataaacgtg tcaatcagca agcacaagaa aacacagatg
    15421 atgttgaact gcaaaaaagc aatatctgct tgatcggacc tacagggtct ggtaaaacat
    15481 ttttagcaca aacattagca aaaacattga atgtaccttt tgcaatcgca gatgcgacaa
    15541 gtttgacaga agctggttat gtgggggaag atgtagaaaa cattttgctt aaattgcttc
    15601 aatctgctga ttacaatgtg gaacttgcag aaaaagggat catctatatt gatgagatcg
    15661 ataaaattgc gcgtaaaagc gaaaatgtat ctattactcg tgacgtttcc ggcgaaggtg
    15721 tccaacaagc cttgctgaaa attctagaag gaactgttgc aagtgtgcca cctcagggcg
    15781 gacgtaaaca tccacatcaa gaatttattc agattgatac gacgaacata ttgttcatcg
    15841 taggtggtgc atttgatggc atcgaaacga ttgtgaagaa tcgtatgggc gaaaagacga
    15901 ttggatttgg tacacaaaac caaaaacttt ctgaagacga aagtgtcatg cagcatatca
    15961 ttcctgaaga tttactaaaa ttcggattga tccctgagtt tatcggacga ttacctgtta
    16021 tggcagcttt agagaaattg acaacagatg atctggtacg gatcttgact gagccgaaaa
    16081 atgcgctagt caaacaatat caaaaacttc tttctctaga taataccgaa cttgaattcg
    16141 aacctgaagc gttgagagcc attgcgaaaa aagcaatcga acgcaatacc ggtgctcgag
    16201 gacttcgttc catcattgaa gaaatcatga tggatgtgat gtttgatatc ccttcaaatg
    16261 aaaacatcga aaaagtgatt atcacaaaag aagcagctga attgtctggt gagccaacag
    16321 taatctataa agattcagag aaaaaagcgg gttgatgaaa agaggctgcc gtaaaagtca
    16381 ttttggcttt tgcgacagcc gcttgcttta ttcatcacct tgttaaggag agagaccatg
    16441 aaagtccatc aagctgaaat tatcataagt gcagtggcac caaaacaata tccagataca
    16501 caattgccag aaatagctct ggcaggacga tcaaatgtag ggaaatcttc ttttatcaac
    16561 acattgattg atcgaaagaa tttagcccgt acttccggta agccaggaaa gacccagaca
    16621 ttgaattttt atttgatcga gaacgcatta cattttgtcg atgttccggg atatgggtat
    16681 gcaaaagtat ccaaaactga gcgagcaaaa tggggaaaga tgatcgagac atatttaacg
    16741 actagagacc agctacgtgc ggtcgtttct ttagtagatt tgcgtcataa gccttctcaa
    16801 gaagatatcc agatgtatca atttttgaag tattatgata tccctgtaat tgttgttgcc
    16861 actaaggcag ataagatccc tcgtggtaaa tggaacaagc atgagagcga aatcaaaaaa
    16921 gcgttggatt ttgatcgcaa tgacacgttt attctctttt cttccgttac aaaacaggga
    16981 aaagaagaag cgtgggcagc aatcgaacaa gcaattgaat cataaaaaga ataaaaaaga
    17041 cttaccagtc aaactctttc ataaaagttt ggctggtaag tc
//
LOCUS       DACEUF010000055        17006 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.058, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000055 DACEUF010000000
VERSION     DACEUF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.058"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(<1..124)
                     /locus_tag="IXG20_001799"
     CDS             complement(<1..124)
                     /locus_tag="IXG20_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ1609994.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..539)
                     /locus_tag="IXG20_001800"
     CDS             complement(228..539)
                     /locus_tag="IXG20_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1609995.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(541..738)
                     /locus_tag="IXG20_001801"
     CDS             complement(541..738)
                     /locus_tag="IXG20_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ1609996.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1132..2859)
                     /locus_tag="IXG20_001802"
     CDS             complement(1132..2859)
                     /locus_tag="IXG20_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1609997.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2852..4045)
                     /locus_tag="IXG20_001803"
     CDS             complement(2852..4045)
                     /locus_tag="IXG20_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1609998.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4233..4877)
                     /locus_tag="IXG20_001804"
     CDS             complement(4233..4877)
                     /locus_tag="IXG20_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1609999.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4971..5105)
                     /locus_tag="IXG20_001805"
     CDS             complement(4971..5105)
                     /locus_tag="IXG20_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ1610000.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="IXG20_001806"
     CDS             complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="IXG20_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ1610001.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7236..7709)
                     /locus_tag="IXG20_001807"
     CDS             complement(7236..7709)
                     /locus_tag="IXG20_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ1610002.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8110..8334
                     /gene="nrdH"
                     /locus_tag="IXG20_001808"
     CDS             8110..8334
                     /gene="nrdH"
                     /locus_tag="IXG20_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ1610003.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8493..10652
                     /gene="nrdE"
                     /locus_tag="IXG20_001809"
     CDS             8493..10652
                     /gene="nrdE"
                     /locus_tag="IXG20_001809"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ1610004.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10702..11667
                     /gene="nrdF"
                     /locus_tag="IXG20_001810"
     CDS             10702..11667
                     /gene="nrdF"
                     /locus_tag="IXG20_001810"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ1610005.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11756..12895)
                     /locus_tag="IXG20_001811"
     CDS             complement(11756..12895)
                     /locus_tag="IXG20_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1610006.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13204..13917)
                     /locus_tag="IXG20_001812"
     CDS             complement(13204..13917)
                     /locus_tag="IXG20_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ1610007.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="IXG20_001813"
     CDS             complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="IXG20_001813"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ1610008.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15106..16638
                     /locus_tag="IXG20_001814"
     CDS             15106..16638
                     /locus_tag="IXG20_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1610009.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16829..>17006)
                     /locus_tag="IXG20_001815"
     CDS             complement(16829..>17006)
                     /locus_tag="IXG20_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1610010.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5279 a         3496 c         2908 g         5323 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      541 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      601 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      661 ataatatttt ccactatgag tcgtaccccc atagataagt tgtgtctctt taatagacaa
      721 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      781 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      841 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      901 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      961 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
     1021 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
     1081 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
     1141 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
     1201 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1261 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1321 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1381 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1441 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1501 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1561 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1621 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1681 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1741 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1801 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1861 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1921 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1981 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     2041 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     2101 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     2161 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     2221 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2281 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2341 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2401 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2461 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2521 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2581 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2641 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2701 agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg
     2761 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2821 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2881 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2941 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     3001 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     3061 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     3121 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     3181 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3241 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3301 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3361 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3421 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3481 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3541 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3601 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3661 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3721 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3781 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3841 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3901 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3961 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     4021 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     4081 ttctgaaaaa aagtgaccac actatgttaa catcatgtag tcactttgtc aaaaatgaaa
     4141 gaaatttgct tgtccaaact gaaaaaaact tggctaaatc tttgtaaata aaaggtttag
     4201 ccaagttaat atttttttaa tatttcacac aatcattctg caagctgatg ctccgggtaa
     4261 aaaaagtttg tcatacgctg tagtgtatat tcatttccaa taaaatgaac gatgtcccct
     4321 gccgtaaatt tagcataggg gccaggtgaa acaagcagtt cttcattatg agaaattgcg
     4381 ataactgtgg cgccagtcgc ctgccagata ttcaactcgc taattgaacg gtccagatgc
     4441 attgcttttt ctgataaaga taattcaaac ggaagtaaag gattcatctt atgtattcgc
     4501 ttcgtttgat tgaccagctg atccaataat tgagaaaaat gattcagctc atcttgttgc
     4561 ttctccacac ttgccatgat ttctgctttt gtatcttgga tggattgtac atcttgatat
     4621 tgcaatagga aatcttttgc tttttccttc gaagcaacaa atgccccact tccatgccgt
     4681 acttccatga ttccaagatc taccagcaca ttgatagcct tgcgggtagt ctcaggagaa
     4741 gcattgtacg tactggcaag tgttgagcgg gcatggatct tctcacccac tttatatcgg
     4801 ttttcaacga tacgttcggc taaatcaaca gcaatctgct ggtagcgcgg gcggacgatt
     4861 tttttctttc cagacatttt ctttcctctt tcttttttta ctttccctca tcttactatg
     4921 ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg caacgactga tcatcgcttg
     4981 atctgttcct tttttactgg gcaagctgtt tgacagtcat cacaattctt accggatttt
     5041 aaacgagtat agacaattgc gcctgcactt ccaaagatta acacacttaa taagaatgtc
     5101 gccatcctat tcctctcctt ctaatgacaa tatagtataa aactggttct tggattttgg
     5161 ctgacggaac aaaacataaa cgagtcctat catcagtaaa atagcaagat acgtccctaa
     5221 cgctacctgt ccaccctcca ataagtgtcc caactgataa atcacaaaac taactaaata
     5281 agctaaaccg cattgatagc ctatggcgat ccaagtccat ttcttggcat tcatttcccg
     5341 atggatagcc ccaattgccg caaaacaagg cgcacaaagt aaattgaagg ctaacaatga
     5401 ataagctgct gcgggagtat atgtgtgttg tagcacttgc cagatttctc tgccattttc
     5461 ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca ataatatttt ctttagcgac
     5521 caatcctgta atcgctgcga ctgttccttg ccagtttccc caaccaagcg gctgaaaaac
     5581 aactgccagg attcgcccga aaaaggctaa gatactttgg tcttcagaaa caggttgagc
     5641 ataaaaatta taagaagagc tgaaccagat cacgatacta gaaacaaaga taatcgttcc
     5701 tgctttttta acaaaagact tgctgcggtc ataggtttgt cttaggaccg ctgaccattg
     5761 aggaaaatga tatgcgggca attccatgat aaaaggagct gtatctccag caaacaaacg
     5821 tgtttttttc aagctgattc cagagaaaat aattgcagtc ataccaagaa aataggcaga
     5881 aggagctacc caagattgat gaggaaaaaa ggcccctgct actaaggcaa taatcggcaa
     5941 ctttgcagaa caaggcatga aggttgtgac catgatcgtc aaacgacgat cttgctcgtt
     6001 ttcaatcgtg cgactggcca tgacgcctgg cactccacat cctgtagcaa tcagcatagg
     6061 aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt cgatccatga caaaagcgat
     6121 cctcgccata tatccgcagt cttccagaaa gctcaagcat aaaaacaata taatcaattg
     6181 cggtagaaat cctaacactg ctcccacgcc tgcgatgatg ccgttcaaga tcagctcctg
     6241 catccatccc gctacttgcc attcagctaa caatctgcca acatgttctg gtaaccatgt
     6301 accaaacaat gtgtcattga tccaatctgt ccccattgtt cctactgttt gaatagccag
     6361 ataatagatc agccacatca caaatgcgaa gatcggcaag gctaaccaac gattcgttgc
     6421 aatctgatcg attttgtcac tcatcgataa ttggaaagaa tcatttgaca caacacagag
     6481 tgtacatagt tgggtaataa attcataacg ttcattgacc agaatcgtct cactatcgtc
     6541 gtccaatagt ttctctgtta actggatcag ttgctcgatc tctttttgct gagaaactgc
     6601 tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa tatttcattg cataccagcg
     6661 tttcttgccg ctttgttctc ctagtacatc ttcaatttca tgtaaagctg cttctaaacg
     6721 tggatcatat gtacagggaa caggaataga aggccgcttt tttgcagact cctgaatctt
     6781 ttcgattagt ttttcgattc cttgcttttt caatacgctg attggtaaaa ctggtacacc
     6841 aagaccatag gaaagctttt ctagattgat tttttttcct tcttttttga tgacatccat
     6901 catattcaga ccaatgatca taggaatgtc taaatccaat aactgcgtag ttaaataaag
     6961 atttcgttct aaattggtag catcaataat gttgatgatc agttcaggct gttcctgaac
     7021 caaataatct cttgcaatca cttcttctgg tgtataagga gacaaagaat agatgccggg
     7081 taggtcctga atatgaatgt tcggattttt tttcaacgat ccttcttttt tttctactgt
     7141 tacgcccggc caattcccta catattgatt cgaaccagtc aagagattga acaagctagt
     7201 tttcccgctg tttggatttc cgactaacgc gatattcatt ctttttcctc cctttgtatc
     7261 aagattagtt cggcctctga ctttcgtaac gtcagttcat accctcgcac tgtcagttcg
     7321 attggatctc ccagaggggc tagttttcgg ataactattt ttacatttcg cgtcaatccc
     7381 atatccatta accgtcgctt cactgcgcct tgtccgtgga ttgctacgac gatcccagtc
     7441 tctcctgctt ttaattgatc aagagaagtc cagctttcag aggaagcagt ctttttattg
     7501 acaaagattt gacctagtat ttcccgactt actgccactc gatgactttt taccagtaaa
     7561 atggcattgt tgcctgataa attgacgacg acgattttac ttccggcaac gatcccgata
     7621 ttttgcaaat ggcgttttgt tttttcttct gtttcgattc gttcgacttg ataaacgaaa
     7681 ttctgctttg cctgcgctaa ggtctgcata ttgattcctc tcttgtgaaa taaatcatgt
     7741 attaaaatca ttcattatat gttctaattt tcttacgtgt tacgcaaata aatgagaatc
     7801 attctcacat agaatactct cggtaattga gaatgtcaat tatacttgtg atattatgag
     7861 cgaatcttta gtattgatag ataaaaatga gaaaaaaata tttcttcata gttttccaca
     7921 attttttctc tattaaattt gctattttcc cacacgatac ttgttttcag ctcttttttc
     7981 aaaaacacaa aacttagtat tccacagttt ccacaacaca atatgtagtg tttgttgttt
     8041 gactttttta ctaagagttt tatactatct attatagata ataagaatgt gttagaggag
     8101 gcactcacca tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg taagatggtc
     8161 aaacggtttt taacagaaaa tcaaattgct tttgaagaag tcaatatcga tgaacagcct
     8221 gaagcagtag actggttgaa agcacaagga ttccaaagtg ttcctgtcat tacttctgat
     8281 gcagaaacag ttatcggatt ccgtcctgat cagttgcgta aacttgccag ctaacatgac
     8341 taaaagaatc aaaacgtgat acatggattt gaagggcttg agacagcacc agtttttgtc
     8401 gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc aatgatttgc aatcaagcat
     8461 catcaaatac gaaatgaagg ttgggcgtac gtatgagtct taaaaattta caagatgtca
     8521 gttatttcaa gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc cctttgaata
     8581 aggataaaga agctttagtt gccttttttg aggaaaatgt caaaccaaat acgatgactt
     8641 ttccatctat tatggaaaaa atcaattact tgatcgatca agaatatata gaagcagaat
     8701 tcattcaatt gtattcacca tcatttattg aaaagctata tcaatttttg gacgagcaac
     8761 atttttcttt caaatctttt atggctgctt acaaatttta ttctcaatat gcgttgaaaa
     8821 ccaacgacgg ttccgcttat ctggaaactt atgaagatcg agtagccttc aatgcgcttt
     8881 actttgcgaa cggggatgaa gaattagcac ttgctcttgc agacgaaatg atccatcaac
     8941 gatatcagcc agctacacct tctttcttga atgctggacg taaacgtcgg ggcgaattgg
     9001 tgtcttgttt cttgactcaa gtcactgacg atatgaactc tatcgggcgc tcaatcaact
     9061 ctgctttgca attgtcccgt attggtggcg gcgtagggat tactttatca aatcttcgtg
     9121 aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga
     9181 aattattcga agatagtttc agctattcta atcaattagg tcagcgtcaa ggagctggcg
     9241 tggtttattt gaacgttttc catccagaca ttattgcttt cctttcagca aaaaaagaaa
     9301 atgcagatga aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta cctgataaat
     9361 tttatgaatt gacacgtaaa aacgaagata tgtatttatt tagtccatat agtgtcgaaa
     9421 aagaatatgg tgtcccattc tcttatgtaa atattacagc tgagtatgat catctagttg
     9481 caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa
     9541 aattacaaca agaatctggc tatccttacg taatcaatat tgatacagcg aaccgcgaga
     9601 atccaatcga tggcaagatc atcatgagta atttatgttc agagatttta caagttcaaa
     9661 cgccttctgt gattaacggc aaacaggaat atgaagtatt aggaacagat atctcatgta
     9721 acttaggctc gacaaatatc gtgaatttga tgcaaagccc taactttggt caatctgtgc
     9781 gtacgatgac tcgtgcattg acatttatta cagatgcatc tgatatcgat gtcgtaccac
     9841 cgatccaaaa cggaaataag ctgaatcata cgattggatt aggtgccatg ggcttacaca
     9901 cttatcttgc aaaagaacaa atggaatatg gctcagagga ttcattagac tttacaaata
     9961 tttacttcat gctgttgaat tattggacac ttgttgaaag caacaatatc gctcgtgaaa
    10021 gaaaacaagt tttccataat tttgaaaaat cagcttatgc tgacggcact tatttcgaaa
    10081 aatacgtaac tggagaattc cagccaaaat cagaaaaagt caaagcattg tttgaaggca
    10141 tttttatccc aactgctgaa gattggaatg ctttgaaaca agcagtaatg aaagatggac
    10201 tgtatcatca aaatcgcttg gcagtagcac caaatggctc aatttcatat atcaacgata
    10261 cgagtgcaag tattcatcct atcactcgca tgatcgaaga acgccaagag aagaaaatcg
    10321 gtaaaattta ttacccagcg ccttatctat ccaatgagac gattccgtat tacacttctg
    10381 cttatgatat ggacatgcgt aaagtaatcg atgtttatgc aacggcacaa caacacgtcg
    10441 atcaaggcat gagtttgact ttattcatgc gttctgaaat cccagaaggc atttatgaat
    10501 ggaagacaac aacgaagcaa acgacacgtg atttgaatat tttaagacat tacgctttcc
    10561 acaaaggaat caaatccatt tattacgtac gtacctttac tgatgatgca gaagaaatcg
    10621 gaagcaatca atgtgaaagc tgcgtgatct aatatcatag atagattcgc ttattcatat
    10681 tttaattaaa ggagcattct catgtcagaa acatattatg aagcaataaa ctggaatgaa
    10741 attgaagata tcattgataa atccacttgg gagaagctga cagagcaatt ttggctagat
    10801 acacgtatcc cactttcaaa tgacctagat gattggcgta cattgtctca attggaaaaa
    10861 gatacagtag gacatgtatt tggcggtttg acactgctag atactgtcca atctgaaagt
    10921 ggcatggatc agttgcgtaa agatgtccgc actccccatg aagaagccgt tttgaacaat
    10981 attcaattca tggaatccgt tcatgccaaa agttattctt ctatttttag tacactgaat
    11041 acgaaaaaag aaatcgaaga aatttttgac tggacaaata cgaatcctta tctgcaaaaa
    11101 aaagctgagc gtatcaatga aatctacaaa cacggtacac cgctagaaaa gaaaattgcc
    11161 agcgtctttt tagaaacatt cttgttctat tcaggttttt atactcctct ttattattta
    11221 ggaaacaaca aattggctaa cgtagcagag atcatcaaac taatcatccg agatgaatct
    11281 gtacatggaa cctacatcgg ttacaaattc caattaggat tcaacgagtt acctgaagca
    11341 gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt atgagcttta cgaaaacgaa
    11401 gaacgttata cagaagaatt atacgatcca atcggctgga cagaagaagt caagacattc
    11461 ctacgctata atgccaacaa agcattgatg aacctaggga tggatccact ctttccagat
    11521 actgccaatg atgtcaaccc aatcgtaatg aacgggattt caacaggcac aagcaaccat
    11581 gacttcttct cacaagtcgg aaacggctat ttgttaggtc atgtagaagc gatgaaagac
    11641 gacgattatt tgattggatt ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa
    11701 taaaaagagt ttagcgtaca gatattttaa atgtacactg aactcttttt tatgcttatt
    11761 cacttgtttc ttccagcact tttggactgt ctaacttttc cgaaacatgt tccattcttc
    11821 tcaataaata gattttccgg atatttaata ttatcgtctt aatcactgca tagacaggta
    11881 tgcaaatcag catgcctaat attcctgcga cacttcctgc tccaatcaat accaagatga
    11941 tcgtcaaagg atggatattc attgatttcc caaataacaa cggcttgatc aaatttccat
    12001 ctaattgttg gatcactaaa atcgacaacg ccatatacaa agcttgaaat ggagagataa
    12061 acaaaccgac aatgattgct ggtaccgctc caataaaagg gccgacatac gggataatat
    12121 tcgtgattcc gcaaatgacc gcaagcaaca agctataagg ctgatcgaat attctcattg
    12181 ccaaaaagct cattacacca ataatcaaag catctaagat cgtactgctg atataggcag
    12241 ataatgtttc gtttaattct tttaccgttt ggcgtaactc tttacgaatc gctttcggaa
    12301 agaagtgaga caacgcatca ataaatttgt gcccatcctt gaacataaat agcaaaataa
    12361 atggaacggt aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt tttgagacac
    12421 ttgcagataa actgactaat atgatattga acaaattact aagagataaa tttgctgatg
    12481 ctaattgttc ttccaaattg aaatttttaa actcttcccg ctgcgccaat tcatttaacc
    12541 atttgcttgt ttcatctgca tacattggca agctctgggt caattgaatt gtctgttcca
    12601 ttaactgagg cactacattc atgacagtta aaacaactaa aatgattacg accaaaaacg
    12661 tcaataaaaa accgaacact ctcgggatcc gatgcttttc gagaaaaaca acgattggat
    12721 cgaacatata atagataaaa ccagcaacaa gcaatggtaa aaaaattgcc gaaagcattt
    12781 tcaatattgg actaaaaata ttcggcattt gtaaaataaa gtacacgccg atgatcgtga
    12841 gtactagctc cactgtccag aaaaataatt ttgattgttt aaaacgtgat tgcatacatc
    12901 tcctccaagg tcctttgata cttctattct atcatttaac aaagagttac gcgctgagat
    12961 tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag acgagtactt
    13021 aaacaaaaac acggtttgga gtagtgacaa aaattatact gcagtccctt tttccaaata
    13081 aacggtgttc aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa atataaggaa
    13141 ctatttttat ttggctgggc ttattacttg atgtaggaca tctattttac aacctcagat
    13201 catttattgc gcaatactga ctaaatgcaa ctttgtgata tttttgtgta cggacaactc
    13261 ctcgatcaca tcggcataag tgagcccttt tggaagatca atcgtataga tatttttgta
    13321 cactcggtag tcatcaacga attttacgtc aaaattcaca tcttcgatta cgatattttt
    13381 ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt ctatggataa attggatttc
    13441 taacttttta gtcgtaggta catggatcac gcgtttcact actgtgagtg caatagcaat
    13501 cccgataaaa ctgatgatcg caatggaata gtaacccatg ccgatggcga tccctaatcc
    13561 agcaattgcc cataaagaag cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat
    13621 gatcgttccc gcgcctaaga aaccgacacc actcaccact tgagcaatca gccttgcttc
    13681 atctgaacga atcgttccag caagctgtgg atactctaaa gcatctctta atgcaccact
    13741 agcgatctca acttggatca atgcaatgat acatgctccc atacaaacta aaatatgcgt
    13801 acgcatacct gctggacgat ttttatattg tcgttcaaaa ccgattgctc cgccaaatag
    13861 catagcgatc cctaaacgta aaataatctc tggaattgtc aataccatat cattcatcta
    13921 aacaccctcc agtccaatat atcatgtctg aaaggagcag acaaaaaaag tgcaattttt
    13981 acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt atgaataaac
    14041 ggtgttcaat cagctgaccc tcggtattac tcaacaaacg gcaaaatatg gggagctatt
    14101 tttcctttgt cttctctaat actcaggtca aaacgctgat ttcacaacct ctttagccta
    14161 ctattgaaaa ttacaactta cttgctgctt cttcatcgat gatcacaaca acattgtcgt
    14221 gattttgcaa tacacttgct ggcaaatgat tcgttacagg tccttcaatc atccctttga
    14281 ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat ttctttactt ttcataattg
    14341 aaccgattcc cattgaaaca gcttttgttg gtacatcttc tgctttatcg aagaatcgtt
    14401 tgtttgcatt gattgttgat tctgttaagt caactactga agtcgttccg tcaaatgaag
    14461 caccaggttc attgaaaccg atatgtccat tttgcccgat tcctaagatt tggatatcga
    14521 ttggatgtgc atcgatgatt tgatcatact ttttgcatgc tgcttccaga tcttctgctt
    14581 ttccatctgg tacaaacgtt tctttgaatg gttttttatc aaataattgg acatccatga
    14641 aatgacggta gctttgttca tctgaaccgc ctagtccaac gtactcatct aagtttacag
    14701 aagtcatttc actgaaatcc aaatcgcttg aagtcatttc tttatataaa gtaactggtg
    14761 tgcttcctgt tgccaatcct aatacttttg cacctttgtt cataccttct ttgatgattt
    14821 caaatgcttt ttttccgcct tcttcagcgt tttttacacg aatgatttcc atagtgattg
    14881 ctcctttata ttttggtata gcccaattat agcattcttt ttttcttttt acaataatta
    14941 gtatagacca aattgcattt ttttcatttt cttgacaata aataggaatc cgtgttaggt
    15001 ttcataagat ggttcaatca aacaatccat gttgcatgaa aaccaagcaa gtgattcact
    15061 gtggacgctt tatgtctgca gtttttttat gaaaaaggag ttattatgag tatactgaca
    15121 ttaaacaaca ttacccagca atttggcgac aaaatcctct atgaaggtgt cgatctgcaa
    15181 ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg gagccggtaa aagtacattg
    15241 attaaaatca tcactggtga gatcttacca gatgacggac agatccattg gcaaaaaaat
    15301 attcacaaag gttacctcga tcaatatgtg gaagtcgatg aaacattaac aatcgaagaa
    15361 tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag aaaaaattgc ttttctttac
    15421 caaacatata gcgagacctt cgatgattcc ttgctggaaa aagctgggaa actccaaacg
    15481 gaattagacc aaggagtgtt ttatcaaatc gacaccttag ttgctgagat gagcagcggg
    15541 ttggggatcg atgtcttagg tttagattca ctgttgaaaa atctaagtgg tggacaacgt
    15601 tcaaaggtga ttttagcaaa actactttta gaaaacccag atgttctgat cttagatgaa
    15661 ccaaccaatc acttagatga tcagcatatc caatggctga cgcagtttct tcaggatttt
    15721 gacggtgcgt atatcgtgat ttcccatgac caagaatttc ttgatcagat caccacccat
    15781 atcgcagaca tcgagtttgg aaagattact aagtatactg gccatctaaa acaagcattg
    15841 aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat atcatgctca acaaaaacaa
    15901 atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg gttcccgatc aacgatggca
    15961 aaaagccgac aaaaacaatt agataaaatc gaacggctga cgcctccagc ttcagcaagt
    16021 aaagctgtct ttgattttcc attctcgcct attgtcacga cattagcagt ggaaacaact
    16081 gatttagtca tcggatatga tcgaccgcta cttgcaccca tcaatttgac catccgcttt
    16141 ggtgaaaata ttgccattcg aggcttcaac gggattggaa aatctacttt attaaaaaca
    16201 ctgatcggtg agattgaaaa aataagtggc gatttccact tcccagataa cacaaaaatc
    16261 aattactttt cacaagatct gtactgggaa aatccattag aaacaccact acaatattta
    16321 agcagtcaat ttccaaaagc gactatcaaa gaattacgtc gccaactagc taaagcaggg
    16381 ttagttaatc aattagcaag tgaacctctc actacattaa gtggtgggga acaaactaag
    16441 gtgaaacttg cacagttgac aatgaaccaa ggtaatttac tgattctcga tgaaccgacg
    16501 aaccatatcg atcaagaaac gaaagaaagc ttacaagacg ggatacagaa atttccagga
    16561 acagtaatca tcgtctctca tgaacaagaa ttttatcaag atcttgtgca acgagtcatt
    16621 gagattgaac caaaataaac gtttgattga atagttgaaa aaaaggagct gtgacaaaag
    16681 ttttgtcaca actccttctt ctgaataaat ggtggccaat tagctgacct tcggtattag
    16741 ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg ctattcttat tacttgatgc
    16801 agaacagctt ttcacaacct cttttcgtct attttttatt ttcgagattt ctctttcctt
    16861 taaaaacaac tgtttgaaag aagaacgcac atccttgaaa aacaagcccg ctaaccgtta
    16921 cacctaacca gccatactgc tgccacattg tcgtccctag caatgaacca gccgcacctc
    16981 caataaagta cataaacata aataca
//
LOCUS       DACEUF010000056        16633 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.059, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000056 DACEUF010000000
VERSION     DACEUF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.059"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(418..1074)
                     /locus_tag="IXG20_001816"
     CDS             complement(418..1074)
                     /locus_tag="IXG20_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1610011.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            1267..1545
                     /locus_tag="IXG20_001817"
     CDS             1267..1545
                     /locus_tag="IXG20_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610012.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1564..2772
                     /locus_tag="IXG20_001818"
     CDS             1564..2772
                     /locus_tag="IXG20_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1610013.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2920..3285)
                     /locus_tag="IXG20_001819"
     CDS             complement(2920..3285)
                     /locus_tag="IXG20_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ1610014.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3432..4205)
                     /locus_tag="IXG20_001820"
     CDS             complement(3432..4205)
                     /locus_tag="IXG20_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1610015.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4379..5239)
                     /locus_tag="IXG20_001821"
     CDS             complement(4379..5239)
                     /locus_tag="IXG20_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ1610016.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(5239..6657)
                     /locus_tag="IXG20_001822"
     CDS             complement(5239..6657)
                     /locus_tag="IXG20_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1610017.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6714..7163)
                     /locus_tag="IXG20_001823"
     CDS             complement(6714..7163)
                     /locus_tag="IXG20_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1610018.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7608..7871)
                     /locus_tag="IXG20_001824"
     CDS             complement(7608..7871)
                     /locus_tag="IXG20_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ1610019.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7970..9265)
                     /locus_tag="IXG20_001825"
     CDS             complement(7970..9265)
                     /locus_tag="IXG20_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ1610020.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(9262..10533)
                     /locus_tag="IXG20_001826"
     CDS             complement(9262..10533)
                     /locus_tag="IXG20_001826"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1610021.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10698..11567)
                     /locus_tag="IXG20_001827"
     CDS             complement(10698..11567)
                     /locus_tag="IXG20_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ1610022.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11859..13469)
                     /locus_tag="IXG20_001828"
     CDS             complement(11859..13469)
                     /locus_tag="IXG20_001828"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ1610023.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14662..16002)
                     /gene="glnA"
                     /locus_tag="IXG20_001829"
     CDS             complement(14662..16002)
                     /gene="glnA"
                     /locus_tag="IXG20_001829"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ1610024.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(16030..16422)
                     /locus_tag="IXG20_001830"
     CDS             complement(16030..16422)
                     /locus_tag="IXG20_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1610025.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4751 a         3529 c         2831 g         5522 t
ORIGIN      
        1 aaaaagggct gtgacaaaaa tcgtgtcaca gccccttttg ttgcaaataa acggcgttca
       61 aaagcttaca tctacggtga taagtccagc caagttaaaa acataagaga ctgtttttac
      121 ttggctcttc ttattacttg atgcagaaca gctttttcac aacctcttgg taaacggtgt
      181 tctcctacca actcctcggt cacaagtcac aatattcatc aaacatgaaa agctgttttt
      241 tgaaattgtt ccttgtgact cagagttaaa cggcaggctc acaacctctt ggtaaacggt
      301 gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaatatgag gaactatttt
      361 tccgtttgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct ttgttcatca
      421 aaagataata ttgtcttcta acaagtccat attcaaaata acgatatgat ggtctttaac
      481 ggtgataata ccatcttttt tcagttgttt taaaattctg tttacactgc tagcagttga
      541 gatgccacaa aatttggcga tttcttcatt cgtgatcgaa aaatcgatca gcatcccttc
      601 ttctgtccgc acaccgaata aatcatataa ttcgtaaagt tgcgtacaaa cagctcccag
      661 ctttccgttt aatagcattt gctgcatttt tttcatcgaa taaagcagac gatagcggta
      721 atactctttt acatagtgtt gtagctttgg gtcttggttg atattttgcc aaaagcgcac
      781 acgattgatt cgataaaact ctgcatattc tgattcaact cgaatattat acggcgaatc
      841 gatgaattgt gaatattcgt cttttaaaag agagacgatt tcaagatctt tgatatagcg
      901 aaggttaaat tctctcccat catttgaaat aacacttgct tttaccgttc cctctttcaa
      961 gatatacgta tgggaatctt ccatcccttc gaatgtcaaa tatttatgat atcctttgcg
     1021 tataatcgga aaatcatttt tatccaaata ttcctttaag actctactat ccaatgatgc
     1081 tcccccatcc aaactttcat ctaaaaagat catacagtac tttaaaactt tttaaagtcc
     1141 aaaggtattt tactaatgat attaacattt gttaagtacc tttttccatc acttatgcga
     1201 tttttttcct tttttttatt cgttatactg cagaagtaaa attttttaag aatagaggga
     1261 aaaaacatga attactggat gaacacaatc attaatcgat tagaaacagc atatcaaaca
     1321 agatttgata tgaaagcttc tttagtcttt ttaaatgatg cgtatcaaaa ctcgatcgaa
     1381 ttgatcaaag cagtggatga gaatccaaca aacgaatgcg aagaattttt aaatctgttt
     1441 atgtctacaa gagatttatt tattcgccaa cttgttgatc gctatccatc caattatcac
     1501 gatgtagaag ttcaaataca aaaattaaaa gcatattcag cttaagggga atgtataaca
     1561 tacatggaaa agaaattatt taataaatat tttattggaa tcacattgat caattttatt
     1621 gtctacctga tttattatct gttgatggtc atcatggcag tcattgcgca agacacctta
     1681 cacgccagct tgagccaagc aggttttgct tctgggatct atatcatcgg aacattgctg
     1741 gcacgcttat tcatggggaa acagttagaa ctatttggtc ggaagtttac cttaagaggc
     1801 ggcgcgatct tctatctcct ttcaacatta gcatatcttt atacgccaac tatcggggca
     1861 ttgtatttag ttcgattctt gaatggattc ggctacggta cagtttctac agctacaaat
     1921 gctatcgtta ctgcatatat ccctgcatct aaaaaagggg aaggcatcaa ttattatgga
     1981 ttaagtacta gtttagctgc cgctgttggg ccatttttag gaatgatttt gctgaatatg
     2041 actgattttc gtttcattat ctggttttca attgtactgg tcttctttgt tacgattgcc
     2101 tgccttgtct ttcctgtaaa aaatattcca ttatctgaag aacaaaaaat atctcttcgc
     2161 tcatggaata tcaatagttt tattgaaaag aaagcacttt tcattacagg aattgccttt
     2221 ttgatgggtc tttcttattc aagtgtttta tcatttttat cttcttatac aaaagtaatc
     2281 cacttagttg ctgctgggtc atttttcttc gtcgtatatg ctttggtcat cacatttaca
     2341 cgtccattga ctggaaggat tttcgatgtc aaaggagaac aatacgtgat gtatcctagc
     2401 tatttattct taacagcagg attattttta ctgagtgtaa cgacgaatag tgtgacattg
     2461 cttatctctg gagcattagt cggcctaggt tatggtacgt ttatgtcaaa tggacaggct
     2521 gtttgtttaa aaatcgtccg cgaacatcga atcagtattg ctttatctac ctactttatt
     2581 ggtttagacc taggcttagg cgttggtcct tatttgatgg ggatgctaaa atctcttact
     2641 tcatttagag gattgtatgt gattgccgga gtcattccat tgatatgtac agcactctat
     2701 cttgttaatg gccgaaaaca tagtcatgaa gcagagggcg aattgattga atcattgaaa
     2761 aatgaaattt gatcttgatg aatagataaa aaaaaccgtt cagatctgaa aataatctga
     2821 gcggtttttt gagtaaaaat agtttatgat tattcttaat taaacatcta tttattttta
     2881 aagctttcat ttatcgataa gaatagttta actgttgatt tattccattt tccgttcatc
     2941 tggtacgatg ccttgactta gcaaccactt gtggaagaac cattctgcca caccgatcaa
     3001 tagagcagga atcaatactt tcatccaaat atcgactgta tcattcataa aatatagata
     3061 gagccacaag ataactgcag ctgaaccagc atctgcgatc gtcgctgcga tgttccctac
     3121 tttacgtaag atcaacagat cccctagcac atagatcatg actacaataa ttaccccaac
     3181 aatcgttgaa tccataaagc taacaccgta taaacctgtc agcacgaccc acagaatcgc
     3241 ccagatcatg attgccttca ctaccaacgc cttcacatgt ttcattttct gcactccttt
     3301 ctcctggata aaaaagtaag attatctaac aagataacct tttcattatt ataccatcgg
     3361 ctgagagaag cgagcaaaga taaggtccta tctaaaaatg aggaagccac ttagaacatt
     3421 cacgtaataa tttatccgta cagacattcg atatggtgtt cctttacttt cgcatactgt
     3481 tcttttgtca gttctttact tgtcaccaga taatcgattt catgaaaagc aatggcttga
     3541 tgcatgaatt tatgtgcaat tttttgctgt gtaactaaaa cggcaagccg gtctgattgt
     3601 tttctaaaag cttttttgac cattgcctct ccttcatgag tttctgttac tccttggctg
     3661 aaatctagcc cgcgacaact cattatcgct aaatccgcat aatatgacaa tgcatcgtta
     3721 taggcttttg aaccgttgat tgttgctcgt ttcggaacaa ttgcccctcc caatacagat
     3781 acttttgccg ttgtatattc actcaacata gcagctgtga cgatgccatt cgtaatgatc
     3841 tgcaaaccat caaatcttgc taggtttctg actaagtgta aagaggttgt actggaatcc
     3901 aagaataaga ccatatcatc tcgaatcaaa gaagaagctg tatctgcaat cttacgtttt
     3961 tctgtttctt cttctaattt ggtgatcttc atcaggtaag gcacgtcaat ttttcgattg
     4021 tcaataagca tggcgccacc gcgaactcta cggatcaatc cacttttttc cagaaaaaca
     4081 aggtcacgtt taatcgtggc ttcgctataa tttactaact cgatcaaatc ggtgactctt
     4141 gcaaattggt tgttttttag ataattcaca atctcctgct gtctattctc cacttcattc
     4201 aacataaaat caccccattt actttatata gtaacaggaa aaaagaggtt gtgacagaag
     4261 tgattggctt cgagaaataa gaagaaattt ttcgaaaaaa ctacttctga acatcgtgtg
     4321 tggttaagag ggtatgacaa gactattgtc ataccctctt gaccacacat ttttattttt
     4381 agtataaact agccttgcct actgtgttca aaatttccat tttatgtttg accacttctt
     4441 gtaccttttt attagctgct ggataaattg cattcggctc gtacatcgat ggattttcac
     4501 tcaatatttc tctcaaagca ttgaaaaaga cgcttttaag atctgagctc aaattcactt
     4561 tggcaactcc gtattttact gcttcgctga cttctgcgtc cggattacct gaaccgccat
     4621 gtaaaacaaa tggaagatcg atttgttggt tcaattcttt cagtaagtcg atatttaatt
     4681 ttggagttct gtttttcgga tacaagccat gcgctgttcc aattgctaca gcaagtgtat
     4741 cgattcctgt ttctttcaca aaaatcttcg cttgatcagg atctgtataa atgattgttt
     4801 cagcacctcc ttcagcagaa ccattattcc caatcgttcc aagctcagcc tccactgaga
     4861 catttaccgc atgagctaac gcaaccactt ttttcgtaat ctgtacattg tcgtcgaatg
     4921 atttcaaaga cgcatcgatc atcacggaag aatagccgtt tcgaatcgcc cgtaagacat
     4981 cttctatcgt accgccatga tccaagtgga taacgatcgg cagtttactt gtataagcat
     5041 aggctcttac tgccgcaaca aactcatcac ttaaataggc gatttcatca ggatgaattt
     5101 ctaagattac tggtgcttct aatctctctg cttcttcgat gattgctttc atcatatcat
     5161 agctgcaaat attgaaggct ggtactgcaa attgattctc tttagccact ttcaataact
     5221 ctttcattgt atataacatt acgcttccac ctcatttgtt agattttcat ttttcgtcca
     5281 cttgtcttgt tttttctctt tcactgtttt tttcaatagt ccaagcataa aggctgtagt
     5341 gactgtacct gatgcaattg ccaagacata cataggtaaa ttgttcataa catttggaat
     5401 tagcatcacc caaatccctc catgaggtgc cattgaagta actcctactg ccatggaaat
     5461 ccctgcagcg actgctgaac ctgtcatcaa actaggtatc acgcggatcg ggtcagatac
     5521 cgcaaacggt atagcacctt ctgtaatata cgaagcgcct aaaatccagc atgattgacc
     5581 tgaagtccgt tcttctttgg taaatttatg tttgaataat aaagtggcta aagcaatacc
     5641 taggggaggt gtcattcctg cagccataca agcagcaatc ggtgcattta ctccagctga
     5701 catcaaaccg atcgagaaag tcgaagtcga tttattgatc ggcccaccca tatcagctgc
     5761 catcatgatc ccgataagta atccgaacaa tagcccggat gtatttccca agctattcag
     5821 ccaatttgtc aaagcatcca acacccatgt gatcggttgt tcaatcacaa ataccatcaa
     5881 aagcccaata attaagatcg ataaaaaggg gacgacaatc agctgataaa tagcagaaaa
     5941 ttcttttttt acgctaattt tatttgccag aaaatcacag atataccctg ctagcacgcc
     6001 accaatcatt ccgccaagaa aacctgaacc acctgtagag gccagtaatc cggcaacaat
     6061 tcctggggca aaacctgctt ttccagcaat cgatgtagca atccccgcgc ctaacgcagg
     6121 aaccatcatg gcaaacgctg tatctcctcc gatccttgaa aatgcagctg ctaacacatt
     6181 atagtctgct gagttgggat cagccgctgt aatcccaaag gcaaaactca gtgcgatgat
     6241 gattccgcct gcgatcacaa atggaagcat gtaattcaca ccattcatga aatgattata
     6301 gattgtcggc tttttctcat ttgcagctgg ttctttttca ttagacacaa cgaatacgcc
     6361 aatatctctc aatgcttcgt ctattactac ttgcggttct ttgattgctc ggcttgtaga
     6421 gacacgcttt acttttttcc cggcaaatcg gtccaattcg atttctttat ctactgcaag
     6481 aatgattgca tctgcctctt tgatttcttg ttcagataac tgattttctg tttttacacc
     6541 ttgtgtttcg aattttgctt gatggtttag atttcttgct gttacttcta atttttctgc
     6601 cgacatataa gtatgagcaa ttccagtcgg gcaagcagtt acaccaacga ttttcatgat
     6661 tattttcctc ctttttcttc actatcgata ctcggacaaa gactctacta ttttcattct
     6721 tgactaatct gtctgatgat atctgctggc gtagtggctt cttttattcc ctttctaaaa
     6781 tctgcattca tcaatcgtct ggagagtgtg ctgagaatct ctagatgcat atctgctgat
     6841 ttttcaggaa ctacgatggc aaaaatatat tcaattggca tttcatctat cccgctatag
     6901 tccgttaatt tggctgtatg aaaaaaacac agtgctggtt ttgtcacata tttgcttttt
     6961 gcatggggaa tcccaaatcc atcttctatt cctgtggaga tctcctcctc tcgtttgaat
     7021 agtgccttaa cgactttttg aacatttctt accttatttg tttttttcaa cacatcccct
     7081 attcgctcaa ataatgatcc ttttgaatct atcgatggat cgaaaacaat cagttcttct
     7141 gcgatcaatt gatcaaacct cataaaaaac ctctccccca caaaaaatgt taacgttttc
     7201 ttggaactga ggtcagtata gcatcgctag ctgtaccgtt caatattatg aatgaacaga
     7261 ggaaaaagac ccatcatccg cgtgtataag tcattaaaat gaacgatcta gctcacaaac
     7321 aatcgcaagt ctataacaaa acaaaaagcc ttccttgaaa ggaaagctta gcttctaatt
     7381 ttaggtaaat ggttttttac ttcgaaataa cccctcttca tttcgttaat ataaggtgtt
     7441 tttatcaagt ggataattaa aactatatac tagtttttca ttgttttgtg cattatgatc
     7501 ctacgacttt ttcagcatca aacgaagttt taaattggca aaaaacataa aaaaacagac
     7561 tatccctatc agatagtctg ctaaacgttg agttatcagc gtttttatta ttccgcagcg
     7621 tttgcgtctt tgagaccgta ttttttgttg aaacggtcca cacgtccgtc tgcttgtgtg
     7681 aatttttgac gtccagtgta gaatggatgt gagtcagaag ttacttcgac acggatgact
     7741 gggtatgtat taccgtcttc ccattcaact gtttcttgtg aagtttttgt tgaacctgac
     7801 aagaatttga aaccagttgt tgaatccatg aatactactg ggtggtaatc tggatggatg
     7861 ttttctttca tgattttttc gctcctttgc cctgctccat ttgctgaacc agagttgatt
     7921 tgtcgattta caacattaac atcttaacat gtttcagtca aggttgcaat tatcttttca
     7981 gatttctggt cggatttttt ttaccaaagg agacatcttg aaattctttg aaaaattgct
     8041 gattgttttt tgttttacgc aaaaagcgga tcagttgttc cgtatattct aatgaatctc
     8101 cagtcatatt ttttcgaagt ttccaaattt cttctaattc ttcacttggc atcaataatt
     8161 cttcttttcg ggtacctgat tttttgatat cgatggctgg gaagatccga cgttcagata
     8221 attcacgtga aagttgtaat tctagatttc cagttccttt gaattcttca tagatcacgt
     8281 catccatacg gcttcctgta tcgactaaag cggtcgctaa aatcgtcaaa ctgccacctt
     8341 cttcgatgtt tcttgctgcc ccgaaaaatt tcttaggttt atacaatgcg gctgggtcaa
     8401 tcccgccact caatgtacgt ccgcttggtg gaacgactaa gttataggcc cgagcaagtc
     8461 tagtaatact atccatcaag ataacgacat ctcgtttatc ttcgactaaa cgcattgcgc
     8521 gttccaatac taattcagaa actcgagtat ggttttgcgg ctgcagatca aatgtagaag
     8581 ataccacgtc gcctttcacg cttcgttcta gatccgttac ttcttccggt cgttcatcaa
     8641 tcagtagaac gattaattct acttccggat agttttcggt gataccattt gcgatttctt
     8701 ttaatacaga agttttccct gcttttggcg gtgcgacgat caatccacgc tgaccaaaac
     8761 cgattggaga aaagatatcg atcattcggg tagatagtct ttgcggtgtt gtttccagtt
     8821 gaagctgtct ttgcggataa agaggtgtta aggctggaaa atgcggacgt tcttttgctt
     8881 cttccggatc ttttccatta acgctttcca cgtgcatcaa accataataa cgttccgatt
     8941 ctttaggtgg acgggctttt cctgccactt tgtccccatt tctcaaactg aatcgtctga
     9001 tttgtgaggc agaaatatag atatcttctg cacttggtcc ataattgatt ggccgtaaaa
     9061 aaccgtaacc atcttgacca acgatatcca atattccttc catataataa aatccttgtt
     9121 tctctgcttg tgcacgaatc acagctagag ataattcctt tttattcatc ttactgtaat
     9181 aaggaatttt aaattctttg gcataactat aaacttcttt taacgttttg ttctctagtt
     9241 ctgccattgt caaataatcg ctcattcagc tcccacttcc tctatcattt caattgatgc
     9301 ccctaaggct gttagtttct cgataatatg atcataaccg cgtaagatat aatctacatt
     9361 gtaaatcgtc gttgtccctt ctgccaatag tccagcaatc actaaacaag cacctgcccg
     9421 aagatcagaa gccacgactt ctgtgccgtg aagtttattt ggaccattca cgatgatcat
     9481 atttccttcc acggtaatat cagcgcccat tcgtgctagt tccggcacat ggttcacacg
     9541 tttcgcatag atcgtatcga tgatttctga agtgcctgta gtcatcaata gtaacgcagt
     9601 aagtggctgc tgcagatctg tggcaaatcc tggataagga taggtcatga tgtttgcggg
     9661 ttttaattct ttcgaggggt aaacttcgat ttcatcttcg ctgattttca tatggacacc
     9721 aatttcctgc aattttgcta taaagctttc caaatgttca aagatcacat ttttcacttt
     9781 gataccattt cctactgcag ctgctaatgc tagataggtt cctgcttcga tgcggtcagg
     9841 aatcatgaaa tgccgacagc catgtaatgt ttcgactcct tcgatacgga tcacatctgt
     9901 acccgctcct cgaacttttg cccccatatt attcagtaaa gttgccacat ctatgatttc
     9961 cggttctcgt gcagcgtttt caattactgt ctgtcctttt gcttttacag cagctaacat
    10021 cacattgatc gttgctccga ctgaaaccac gtccatgaaa atccgattgc cacgcaatcc
    10081 tttattttct gtccgtaaat acatggcacc atgttcattt gtcacttcag cacctagagc
    10141 ttcaaatcct ttgacgtgta ggtcaatcgg tctagggcct aaataacagc cacctggcag
    10201 gcctactacc gcttcgccaa atttaccaag cagtgttccc ataaaataat aagaagcacg
    10261 caaactattg atttttccgt gcggcatcgg aatcgatacg acttctcgag gatcgatttc
    10321 tagtatgttg ttggaaaaat ggacagttgc gcccatgatc tctaaaattt caattaatga
    10381 atgaacatct tgtatgtccg gcacaccttc caagatcact ggggaatcag ctaagattgc
    10441 agctggaatc aatgcgactg cactattttt tgcaccgctg atcgttactt caccagctaa
    10501 aggtcggttt cctttgatta cgattttttt catctgtttc caccttgttt attaatcttt
    10561 tttcatggat atgcttgtat tttatcatag ataaaaaaaa gaaacgagca tggtctgccg
    10621 ttttaagtaa aaaagaatgg agagcgactt caaaagtcgt tctccattcc tatatatcgt
    10681 atctgttatg tggatgctta cgctttgtta gctgaaccga accagtcgat atgagattta
    10741 gcatcttcaa cgattgctgc tttacctgga gctaataatt tacgagggtc gaatccttta
    10801 ccttctttat ctttaccagc ttcgatatat ttacgagtag ctgcagcaaa tgataattgg
    10861 aattcagtgt taacatttac ttttgagata cccattgaga tagctttttc gatttgatct
    10921 tgaggaatac ctgatccgcc atgtaatact aaaggcatgt ctggacctac agcatcagca
    10981 attgcttgta agtggtcaaa agctaatcct ttccagtttt ctggatattg accatggatg
    11041 ttaccgatac cgcaagctaa gtagtcgata cctgtagcaa ccatttgttt gcattcttca
    11101 atatcagcta attcgccgtt accgatgatt ccgtcttctt ctccgccgat tgaaccaact
    11161 tcacattcaa ctgagatacc ttttgcatgt gctttttcaa caacgtcttt agctaatttc
    11221 aagttttctt caaatggtag atgtgaacca tcgaacatta ctgaagtata gcctaattcg
    11281 atacattcca aagcagcttc gtaatcacca tggtctaagt ggatagcaac tggaacagtg
    11341 atgttcattg cttcaactaa atcagtgatt aaatctttac agattttgta accacccatg
    11401 tatttagctg cacccattga tgtttggata agtactggtg cttgttttgc ttcagccgct
    11461 tcaaggattg cttgagtcca ttccaagttg tttgtgttga atccgccgac agcgtaaccg
    11521 cctttacgag cagcttttaa aaattcagct cctgatacta ctggcataat aaaattcctc
    11581 ctaagttttg taaaactaaa tttgttaaga caattcttaa ccaacgaccc tattttacca
    11641 aacatttcgg tagttttcta ctaaaatcaa tgaaaatgaa aattttcaag aagttttttg
    11701 caaaaaataa aatatccaat agcgctttaa tcgaaaaaag tcttttgctt tctatctttt
    11761 ttcctacaaa taataacaaa tcagaaaaag agaaaagtga caagaaaatt gcttatcccc
    11821 ttctcttgct acttttctct ttctatcaag ttcaaaaatt attttgtttc gtgatctgct
    11881 aatgatgctc cgacaaatgc tttgattaat ttttgcgggc gatttggacg agaaatcaat
    11941 tctgggtgga attggcatcc cacaaagaat tttttctctg gcagctctac gatttctact
    12001 aaacggttat ctggtgagac acctgaaaag actagccctt gttcttcaaa caattgacga
    12061 tatttgttat tgaactcata gcggtgacgg tgacgttctt gcacaacatc ggctccatca
    12121 tatgcttcag ccgtcttcgt tccttttttg attttacatg ggtataatcc aaggcgtaaa
    12181 gttccaccca aattctcgat gttttcctga tcagccatta aatcaataat attgtttgct
    12241 gtatctggat tcgtttcagc tgatgacgca tcttctaatc caacgacgtt acgtgcaaat
    12301 tcgacacaag ccatctgcat accaagacaa atacctaaaa acggaacgtc attttcgcga
    12361 gcataacgaa tcgcttcgat tttcccttct actccgcgat caccaaatcc accaggaacc
    12421 aagattccat ctgcgtcttt caaacgttct tctacatttt ctctcgttaa ttcttgtgct
    12481 ttgatccaat cgatctcgat gtctgaatta taggcgaaac cagaatgttt caatgcttca
    12541 actactgaga tatatgcatc tggtaactcg acatatttac cgacaagcgc aatccgtact
    12601 tttttcttca agttcaagac acgttcttct aattctttcc attctgtcat gtctgcttct
    12661 ggtgtatcta gttttaaatg gtcgcaaaca atttgatcca ttccttgtgc ttgtaaaact
    12721 aatgggatgg agtaaagtgt atctacatct ggcgactcga taactgcttc tggttcgaca
    12781 tcacaaaatt gtgccaattt gtttttcacg ctttgagaaa caggttgctc tgtacgtacc
    12841 accaaaatat tcggttggat acctaaacca cgtagctctt ttacactgtg ctgcgttggt
    12901 ttagttttca tttctccagc agctttcaag taaggaatca atgttgtatg gatatacata
    12961 acattatctg taccgacatc tgctttcatt tgacgaagtg cttcaaggaa tggcaaagat
    13021 tctatatccc ctactgttcc ccctacttct gtaatgatca catctgaatc agtcatttta
    13081 gctgctcgca tgattttttc tttgatctca tttgtaatat gaggaataac ttgaacagtt
    13141 gctcccaagt attcgccctt acgttcttta cgcaaaactt cagaatagat ttttcctgtc
    13201 gtcacgttgg agtatttgtt caaattgatg tcaataaaac gttcgtagtg gcccagatcc
    13261 aaatctgttt cagcaccatc atctgttacg aatacttctc cgtgctgata tgggctcatc
    13321 gtccccggat ccacgttgat gtatggatca aatttttgga tagttacttt taagccgcgg
    13381 tttttcaata agcgtcctaa agaagctgca acaatccctt ttccgattga tgaaacgaca
    13441 ccgccggtca caaaaatata ttttgtcata agttcaaaaa ctccttttta atttatgtgc
    13501 aggaaaagtc tcagacaata aacaaagctc cctattcttc aataagaata gggagccgaa
    13561 atttctgtaa acttctgacc ctctaaaagg gtgcccaagg tgtataatac aagctgacta
    13621 agggaaagtc aagtactcaa atgaatacta tccgcttaca attttttctt tttcgatatc
    13681 ctttgagaaa ttatttctta agtcctttat gagtgcaaaa aaaatttaac ccacataata
    13741 agcttgatat aaacatagct aacgttaaac ctaagcaaat cagaaaatcg ttactgaaaa
    13801 attttaagtg aaataataga taaaaaaata aaacaaacat tatgatagat aatatcccac
    13861 ctaacaaaga actacttttt cctacttgca gcaacagttt atatagctta tcgtagaaac
    13921 aatctttttc tttcaccctt atttacctcc tttacgtaaa aaacgtagtc cacagttctc
    13981 ggatttttgg catatctttt ctattaaata atatcaaaaa caaaacaatt ccactaattt
    14041 tttcaaagat gacctttttt tcaacaaaag tgccttgtag ggattcattg aaggtaacga
    14101 atcacataag taaaaaataa ggagatgcga caaaagtcgt gcctccttat ttctaaataa
    14161 acggtgttct cctgccaact cctcggtcac aagtcacaat atccatcaaa catgagaagc
    14221 tgtttttgaa attatttctt gtgattcgga gttaaatggt aggctcacaa cctctggata
    14281 aacggtattc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtagaaag
    14341 ccattttcac aaaatttttc ttaatactcg aaactaaacg tctttttcaa aacctcttgg
    14401 taaacggtgt tcaaaagctt gcatctgcgg taataagttc agccaattca aaaacaagaa
    14461 aagctgtttt tgcttggcta ttcttattac ttgttgcagg gcagcttttt cacaacctct
    14521 ccactaaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg
    14581 aggaactatt tttccgttag tttctctaat actcaggtca aaacgctgat ttcacagcct
    14641 ctaattcacg gtattcctgt tttaataaag ttctagatat tgttctcttt cccactcgga
    14701 aacagtttgt ctgaatgccg cccattcgag acgttttgct tcaacaaaat ttgagaagat
    14761 atgttgtcct aatgcgtcaa tcattacatt atccttacgc aattctttga tagcattatg
    14821 gatagttgaa ggtaagtcct ggatatgtgc agcttctctt tcttcctcat ccattacata
    14881 aatgttacgg tcaacagctg ctggcggtgt gatctgatta cggataccgt ctagtcctgc
    14941 ttgcaacaat actgccattg ccaagtatgg gtttgcagat ggatcaactg accgcaattc
    15001 caaacgtgtg gataaaccgc gagattctgg tacacgtact aatggcgaac gattacggcc
    15061 actccatgcg acataaacag gcgcttcata acctggaacg agacgtttat aagaattgac
    15121 tgttggattg caaacggctg tgtaggcacg agcatgtttc agcaaaccac ctaagaaatg
    15181 ataagctgtt tcacttagtt ccaattcacc gtctttatca tagaaaacat ttccttcatc
    15241 attgaacaaa gacatattgc aatgcatacc agaaccattg atcccaaata acggtttcgg
    15301 catgaaagtc gcgtgaagtc catgcttgcg tgcaatcgtt ttgacaacga gcttgaaggt
    15361 ttggatatta tcacaagcat ctaccacatc tgcatattta aagtcaattt catgctgtcc
    15421 tggcgctact tcatgatgtg aagcttccac ttcaaagcct agactttcta attccaacac
    15481 gatgtcacgg cggcaatttt cacctaaatc tgttggtgca aaatcaaaat aaccaccttt
    15541 atcattcaag gtcgttgtga tcccaccgtc ttcgtctaat ttaaataaga agaattctgg
    15601 ttcaggtcct aaattgaaag aggtaaaacc aaggtcgcgc atatctttca atgctcgttt
    15661 caaattcccg cgaggatcgc cggcaaatgg tgtcccatcc ggattataga tatcacaaat
    15721 taaacgagca acttttccat gagcactttc ccacgggaag atcatccaag tggagagatc
    15781 tggatacaaa tacatatcgc tttcttcaat tcgaacaaat ccttcaatcg aagaaccgtc
    15841 aaacatcatt ttattttcta atactttgtc gatctggctc actggaactt ctacattttt
    15901 gatcgtacct aagatgtctg tgaacatcaa tcgtaaaaat cgtacgtttt cttcctcaat
    15961 gattcgtttg atttcttcac ctgttatctg tttctttacc atggtatccc tcttcctttt
    16021 ttaatttatt cagtcaaaga attgtctttg caatccttgc gactgataat gacctgtttt
    16081 gcttaaccca ccttgcgcca aaatctcatc acgaaaaatt tgtcgaacgt catgatctgt
    16141 caacggcttc ttgttttttg cttcttgttt tttcgcctgt gccatttcgt atacacgttt
    16201 gatcccagcc atattcaatc cctctgaaag atagtcttta atctcaagca acatatcaat
    16261 atcattcagc gaatacatac gtcggtttcc ttcgcttcgt tccggatgaa ctaaatcttg
    16321 ttcttcataa tagcgaattt gccgagcaga caaatcagtc agcttcataa ctgtaccaat
    16381 tggaaatacg gacatagatc ggcgcaattc cttttctccc atcagacgtc ccctttcgtt
    16441 ttataagttg gctaaagtgt accaattaaa aaaaatattg tcaactgcat atgttaggtt
    16501 ttctaacatg agatctctat tttttataaa ataacaaaaa aataagaaca caaaaaaggt
    16561 atctagttga ttttcgtacc agataccttt tattttttat tatactgtac tatcctttat
    16621 tagtgttttt att
//
LOCUS       DACEUF010000057        16631 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.060, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000057 DACEUF010000000
VERSION     DACEUF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.060"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(45..779)
                     /locus_tag="IXG20_001831"
     CDS             complement(45..779)
                     /locus_tag="IXG20_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1610026.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1105..1515
                     /locus_tag="IXG20_001832"
     CDS             1105..1515
                     /locus_tag="IXG20_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ1610027.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1508..2209
                     /locus_tag="IXG20_001833"
     CDS             1508..2209
                     /locus_tag="IXG20_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ1610028.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2209..3822
                     /locus_tag="IXG20_001834"
     CDS             2209..3822
                     /locus_tag="IXG20_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1610029.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            3812..4033
                     /locus_tag="IXG20_001835"
     CDS             3812..4033
                     /locus_tag="IXG20_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ1610030.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4030..4791
                     /locus_tag="IXG20_001836"
     CDS             4030..4791
                     /locus_tag="IXG20_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ1610031.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5172..5681
                     /locus_tag="IXG20_001837"
     CDS             5172..5681
                     /locus_tag="IXG20_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1610032.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5751..6290)
                     /locus_tag="IXG20_001838"
     CDS             complement(5751..6290)
                     /locus_tag="IXG20_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ1610033.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="IXG20_001839"
     CDS             complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="IXG20_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ1610034.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7357..8154
                     /locus_tag="IXG20_001840"
     CDS             7357..8154
                     /locus_tag="IXG20_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ1610035.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPAATILV
                     EGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLGYEHVIANFPAFTLAYFAS
                     QGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8182..8817
                     /locus_tag="IXG20_001841"
     CDS             8182..8817
                     /locus_tag="IXG20_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ1610036.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8837..9610
                     /locus_tag="IXG20_001842"
     CDS             8837..9610
                     /locus_tag="IXG20_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1610037.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10018..10815
                     /locus_tag="IXG20_001843"
     CDS             10018..10815
                     /locus_tag="IXG20_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ1610038.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            10793..11206
                     /locus_tag="IXG20_001844"
     CDS             10793..11206
                     /locus_tag="IXG20_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610039.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11190..13835
                     /gene="mutS"
                     /locus_tag="IXG20_001845"
     CDS             11190..13835
                     /gene="mutS"
                     /locus_tag="IXG20_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1610040.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13853..15961
                     /gene="mutL"
                     /locus_tag="IXG20_001846"
     CDS             13853..15961
                     /gene="mutL"
                     /locus_tag="IXG20_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ1610041.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15983..16543
                     /locus_tag="IXG20_001847"
     CDS             15983..16543
                     /locus_tag="IXG20_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ1610042.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5399 a         2969 c         3602 g         4661 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaaaacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc gagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctaa ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 ttcacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaagaaac atcaacacga aaagcatcgg
      841 tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata aaaaaatgaa
      901 ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt aaaagttctc
      961 cacaaaccac caagcttaac tataaatata ccaaaaaaaa aacgtttttt ttctcaaaaa
     1021 ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag taaaatgaaa
     1081 gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat tattattttc
     1141 ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc tggaagtatc
     1201 ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc tgaaaaaatc
     1261 ggagcaactg gtgatttttt attgaataat ctagctattt tatttgttcc tgctggtgta
     1321 ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact gggagcagtc
     1381 gtcgtgtgtt ccatcgtcac gatggttgca gagggaaaaa cggcagaagg tgtagaagct
     1441 ttgctgggct atgtccgtaa gaaaaaagca ggaagggtat ctgtaaacga aatggaggaa
     1501 aatcaaattg actgagtttt ttgtgaatcc actatttggc ttaagtttga ctttaggagt
     1561 gtatgtatta tctgataaat ttttgcggaa atataaactg cctgttttga atcctctcgt
     1621 attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct ataaagacta
     1681 taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg cacttgccat
     1741 ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa tggctgcgat
     1801 tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact tcttctctct
     1861 tcataaagag atggtgatct cattactacc taaatctgta acgacggcta tttctgtcga
     1921 tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg tctctacagg
     1981 tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg aaagtccagt
     2041 tgctcgcggt gtggcgttag gaagtacttc tcatgctatc ggtacagcaa aagcaatcga
     2101 gattggcgaa atagaaggaa tcatttccgg acttgcgata tgcgtaaatg ggatattgac
     2161 cgttttgttg ctgccgttat tctttcagcc ttttgctggc ttgttttgat gcgaaaacca
     2221 ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa agacgtgtat
     2281 ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat tctcctccta
     2341 gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt tgttcagctg
     2401 gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca gcagtttagc
     2461 ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt ttatgatacg
     2521 ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac gctgaaattc
     2581 cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt tgcgcaaaag
     2641 cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca gcaactgcaa
     2701 acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga agaaatcatt
     2761 cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg gccatttgaa
     2821 acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt ttttaatgtt
     2881 cgtctgaacg agatcaaaaa aagacgattg gaatacatgc taggtgcctt ttttttaaga
     2941 aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat ttctgacaat
     3001 ctgttgttcc agcgattttg ccaagtcatg gagccggtat ttcctaatta ttttcaagta
     3061 gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata ttatagtgat
     3121 cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc gccctttaca
     3181 gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg gctgccagca
     3241 gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt cgcctatctt
     3301 tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa tcatttagtt
     3361 attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag acacaaacat
     3421 cccaatcatc tggcatttaa aaatcatgcg tttttgatgg gtcggtatct gactgtgttt
     3481 aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt aatgacagat
     3541 ttcccattat ttgaagagca actgttagag gagggattac ggacattttt ccgtaatgag
     3601 tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct tctgatttcg
     3661 acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt gaccgaagaa
     3721 ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca aaaaaaaact
     3781 attaaaacaa taacaaagaa ggagaaaaaa gatgacattt gaagaaattt taccagaatt
     3841 gaaaaaaggt gcaaagatta ttcgtaaagg atggagtgga tttgaacttt atgttgttct
     3901 cgtatccgat gatgaatttg atggctgtcc agtgacacct tacttattga tcaaaacaag
     3961 tgacgaaggt ttttccagct tcgctcctac agtttgtgat attttagcag atgattgggc
     4021 gatcgtagaa tgaaaaacca gtatccagat ctagctaaac aggttgtatt tatcacagga
     4081 gctgcttctg gaattggtgc tgcgcaagcc cgagcgtttc ttgaacaaga tgcttttgta
     4141 tttggattag atcaaaagtt cggtaaaatg gaagaactct ctttgcgatt tcctgaaact
     4201 tttgcctacg cactaggtga tgttcgacaa atgaaagaac tccaacaagc ggtatcgaaa
     4261 tgccaggatc gttttggtga agttacgatt ttactgaata catcggggat attggatgcc
     4321 tacaaaccat tgatggaaac tgacgaatcg ctgtgggatc ttatttatga gacgaatgtg
     4381 aaaagcatgt atcagctgac aaaaatcatc cttcctgata tgatccgccg aaaaagcgga
     4441 acaatcatca acatggcttc gattgcgggt ttggttgctg gcggaggtgg gatcgcttat
     4501 acaagtgcga aacatgcaat cgtcggattt accaaacaac tcgctctgga ttacgcagga
     4561 cagggtatcc gagtaaaagg aattgctcca ggagccatcc aaacaccaat gaatgcggct
     4621 gattttgaag ggaaaggtga gatggctgag tgggtcgcaa atgaaacacc agtcaagcga
     4681 tgggcacagc cggaagaagt agctgagctg acgttatttc ttgcctctcc tcaagcaagc
     4741 tacatacaag gaacgatcat cccaatcgat ggcggatggt tgttaaaata accttgtgtt
     4801 tttttcaagc ttctgtatga taaaactaaa gagagaaaca acggttgatg ataaaagaaa
     4861 gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt ctttttaata
     4921 atcgttaata ttcgaaacta agcggctttt cacgaccttt tgatataaac ggtgttcaaa
     4981 agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct atttttattt
     5041 gactattctt attacttgtt gcagggcagc tttttcacta ccttctgcgg cacttcagtt
     5101 ggaattttct tcccatcctt tggtcgcagt cattaccaag ataaaattat cttgcaaagg
     5161 agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca acgacagatg
     5221 tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt ttgtctgtct
     5281 ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg tccttgtata
     5341 acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatg gcttctcctc
     5401 ttggactgat agatgttgta gtaggaagcg tcagcacgct gcttgtattg cttatcaact
     5461 ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggctgtgaca gcatgcgtat
     5521 ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa ctaccttttt
     5581 tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc ggtgggataa
     5641 ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt taatttaaaa
     5701 tatggcagcg tggaagagac tttgagtctt cttccatgct gcttttttga ttaatttgta
     5761 tgaagaaatc gatttagccg ttttttcatc aagataccga tataagctgc tccagttgcc
     5821 ttgattgctt ccggcagtaa aaacggcagt agtccactgg caaatgcagt tgtaaaagta
     5881 agatcaccac ttattttcag ccagatcgtt ccgcagatca atgcagcaag agagcccatt
     5941 agattggcaa agattgcata taagtaatga gcatccgttc tttcgatcat ccaaccagtt
     6001 atccacgttt gtaagagaaa tccaatcaaa tatccgccag ttggcccaaa taaaacagct
     6061 atacctccag tcatccctga aaatacagga agaccaatca agccaagtaa aagataaact
     6121 acaacagata tcaatcctag ttttcttccg agaaaagttg ctgttaatcc aatagcaaat
     6181 gtttgccccg tcaaaggaac caagccaaaa ggaatggtca tctgagaaag gacagaaata
     6241 atcgcggcaa attccgcagc aagtattaat tctttcaatg ttcggttcat aatgccctct
     6301 tttcgttaac ttaatatttt tatttggtta acgaaattgt aaaacaaagt gattactttg
     6361 taaatagaaa atataaaaaa agaccagcta tccaatagat agcggtcttt ttcaagaaag
     6421 cttttgaaat tataagcttt ggttgtagta ttcaacgaca agagcttcgt cgatttctgg
     6481 atatagttcg tcacgttctg gtaaacgagt caatgaacct tccaattttt cagcgtcaaa
     6541 gcttacgaat gctgggcgtc caacagttgc ttctacagct tcttttactg ttacgatgtt
     6601 ttgtgatttt tcacgaactg agatcacttg acccacttct acgtggtatg aagggatatc
     6661 aacgcgtttg ccatcaacta aaatgtgacc atggttaact aattgacgtg cttgacgacg
     6721 agtcgttgca agtcctaaac ggtaaacaac attgtctaaa cgttgttcta gtaagatcat
     6781 gaagttaaca ccatgtttac cttctttgat tttgctagct tttacgaaca agttacggaa
     6841 ttgacgttcg ttcattccgt acatgtgacg aagtttttgt ttttcagtta attgcatgcc
     6901 gtattctgat tgtttgccac ggctgttagg tccatgttga cctggtgcgt aagggcgacg
     6961 tgctaattct tttcctgtgc ctgacaatga gatgcctaag cgacgagaaa ttttccatga
     7021 tggtcctgta taacgagaca ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa
     7081 taatccgttg aaaaattcat aatcatatag tttgttcttc aatcttcacc tttgcagccg
     7141 cggttacaca atcgaacccg agacggggca acaaactagg gatgagttat tatttttctg
     7201 ctgcattatt ttacacaaac tacagtatat gatagatcat ccacgaatgt caagataatt
     7261 agtacataaa aacgctttac aaacctaggc tgtctttata aaatgagagg gcatgtgcaa
     7321 atggaaaaaa gcgaaggaag tttaaggaga aaatcaatga atccggtatc accattattt
     7381 gagaaaatcg atgcttcaat tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt
     7441 tacgctgttc gagcggttct cgcctgtttg tttttgactt taggaacggc tgtggcattt
     7501 gcaatcgcga tgaaggggga agatatcgcg catggtctag gaaagatttt atacgcattt
     7561 atgttcagct ggggtctggt catgattcta tacatgaacg ctgaattagg aacatcgaat
     7621 atgctttata tgaccgtggg tgtttttcgg cagagacttg ctttaagtaa ggcagccaag
     7681 attctatttg cctgtatttt tttcaatatg attggcggta tattatgtgg atttcttatt
     7741 tctatgacta cgccatttca agatttaacg ttggacaact ttatgttaga atcaatagca
     7801 gtaaaactag caaaacctgc agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt
     7861 gtcaataccg ctgtactgat cagcatgcgg atgaaagagg atgccgggaa agtgatcaca
     7921 gtcgtattca tcattttcat ttttgctttt ttaggatatg aacacgtgat agcgaatttt
     7981 cctgccttta cgcttgctta ctttgcttct caaggacaga cggatggaat gacagtcagc
     8041 aatgtgttac ataatttgtt ttttgctttg gcaggaaatt atattggtgg cggtttagta
     8101 atgggactgg gatatgcttg gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc
     8161 gttcattaaa ggggaagtgc catggaagtt ttagtttttc caaaaaatga ggaaaaagcg
     8221 ttacaaatgg aaaagtacat gaaaaatctt ttttcctttg ctggagtacc taagccggaa
     8281 cgcgctcttt tacaacaacc gttactggat gagagcaaaa atttatctat ttctgaagtt
     8341 ttagcttttg tttattatta ttatgaacaa atcgaaagag agtatcagta tgtagcgatc
     8401 gatttagtcc agaaaaacat caaacggctt acatttgaag acttagtcgt attgttgcca
     8461 ctagttcaga aaaaagaatg gtgggacagc atcgactctt ggagaaaagt atacagcaca
     8521 tggtgtaaag cctacccaga gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa
     8581 gatttttggc tacgtcgaat agcaatcacg ttgcaattag gttataaaca gcaaacagac
     8641 acaggactat tgatccgagc aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa
     8701 aaagcaattg gttgggcatt acgcgattac agtaagaccg atgccgattg ggtaaaaaga
     8761 cagctgacaa cagatttgag taagttagct gttcgagagg caagtaagta tttataagaa
     8821 agcgagacga caagagatga gacgcaaatt atttgctttt gatattgatg gaacgttgtt
     8881 agattcaaac aaacaagctt tagatagcac gagagaagca ttggaaaaat tacgcaaagc
     8941 cggtcatctg gtcacgattg ctacagggcg gagccgattt catgcgcaag agatcattcg
     9001 cgatcttgat tttacgaact acattttatg taatggagcg gccggttttt tagaccatga
     9061 acaagtatat aagaatttac tagatcgcga ccagttggat gcgttcgttc aagaagcgca
     9121 tcatgagcag atcgatacag cttttgtcag cttagacaat atcaagcgtt cttcttccaa
     9181 tgatgtaacg gtgatggaag aagcgatgca ttctttcggt gctgtcttgc ctgaattaga
     9241 tcagtttttt gcagaggaac aagaaatata ccaagcgtta gcatttttcg atcaaacata
     9301 tgatgggaag ttctcttcat atgataaaat tcgatttgtc cgctggcatg agaacagtgt
     9361 agatgtcgta cctaaaaatg gttctaaagc agcgacaatt ctgcacttag cagaacaagt
     9421 aggcatccct catgaagaca tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt
     9481 gcgcatgtca ggaattggga tagctatggg gaatgctgtg ccagaagtac aagcagaagc
     9541 taagatggtc actgatacaa acgatcagga tggaatttgg aaggcattga aagagttaag
     9601 cgtgatttaa aagaggttgt gaaaaagctg ttctgcatca agtaataagc ccagccaaac
     9661 aaaaatagct cctcctattt ttcgtttttc tgggcttatt accgcagatg caagcttttg
     9721 aacaccgttt aaggaagagg tcgtgacaga agtggtcagt ttcaagaaat aagaagggat
     9781 ttacgaaaat tgcttctcaa atttttgtaa aattacggct tatttctgaa gaagtagatg
     9841 aaacaacgtt tattcgggaa atgggactgt gacaagatgt ttgtcatagt cccatttttt
     9901 tattttattt ttcaaaacta agaaatacca ggaaagagtt ccttttgttt gaccttttct
     9961 ttcttattga aaacaactat aatatagagt gatgttaaaa gagaagagga atttagtgtg
    10021 cgggtattgt ttattggtga tgtagtaggt tcaatgggac gagagatgat tactgaatat
    10081 cttcctagat tgaagaagaa gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc
    10141 gcttctggac gtggaatcac agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat
    10201 gtcgtgacga tggggaatca tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa
    10261 gctaaaaaga tggttcgtcc ggcaaatttt ccggaaggga cgcctggaca agggatggtc
    10321 tttgtcaaag tgaaccaagt cgaactggca gtgatcaaca tgcaggctcg ttcttttatg
    10381 gtggacttgg atgatccttt tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt
    10441 acgccgatca tttttgtaga ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc
    10501 tggtttttag atgggaaagt ttcagcagtg gtcggtacac atacacacgt tcagacaaat
    10561 gatgcgcgaa ttttgccaag aggaacagcg tatctgacag acgttgggat gacaggtccc
    10621 tatgatggta ttttgggtat gcgcagagaa cctgtaatcg aaaagtttct gaccgcatta
    10681 ccaaaacgtt ttgaagttgt ggaacaaggc cgatcgattt taagcggctg tatcttggaa
    10741 ttggatgata cgacaggtca tgcaaaaaaa atccagctga ttcagatcaa cgatgatcga
    10801 ccgttcatgg agtagaaaga gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga
    10861 agtaaggaaa ctgacaaaac ttttaagaga gaatgaaacg atcattcgct acaaagaact
    10921 tgaagaaaag atccagcaaa accagtattt agctgagttg agagaaaaaa tcaaacaagc
    10981 gcaaaaagat gctgttcatt ttgcccacta tgacaaacca gcagcagaac aagaagcaat
    11041 caaacaagcg gatcaattca tgcaagaatt tgatcagcat ccgcttgttg tagcttatag
    11101 aaaacagctg ttggaagcag atgatctgct tcatcattta acaactatga tacaagaaga
    11161 aatcaatgga caaatagagg aggaaaaaca tgcctcaaaa aactaaaaat actcctatga
    11221 tggaacaata tctaagcatc aaggcccaat accaagatgc ttttttgttc tatcgtttag
    11281 gagactttta tgaactattt tacgaagatg cgatcaaagc gtcacagctt ttggaattaa
    11341 cgttgacgag cagaaatcga aatgcagaag aacctattcc tatgtgcggt gtacctcacc
    11401 atgcagcaca aggatatatc gatgccttga ttgaaaaagg ctataaagta gcgatttgcg
    11461 agcaggtaga agatccaaag acgactaaag ggatggtcaa gcgcgaagtg atccagctag
    11521 tcactcctgg tacagtcatg gacagcaaag gattgtcggc aaaagacaac aattttttaa
    11581 cagctgtagt tagtcaagga aatgaatttg gctttgctta tgccgattta agtacgggag
    11641 aattgaagac ggctcggttg catgatgaag aagctgtttt aaatgaagcc tctgcattgc
    11701 agacaaaaga actcgtatta ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg
    11761 ggcgtttagg gctagtcttt tctcaacaaa atgagatgga agaaaacgcg gaatttagct
    11821 ttttgacaag cgaactagtc gatccgctga aaaaagaagc aaccggcaaa ctattgactt
    11881 atttatcggt gacacaaaag cggagtttag ctcatatcca aaaagcagta gaataccaac
    11941 cagatcattt tttgaaaatg gattattatt caaaattcaa cctagaatta agccaatcta
    12001 ttcggactgg gaaaaagcac ggcactcttc tatggttgtt agatgaaaca aaaacagcaa
    12061 tgggtggccg tttactaaaa caatggctag accgtccgct tatccaggca aaacaaatta
    12121 aagcaagaca agaaatggtc gcttctttgt tagacagcta ttttgaacgt atcgatttgc
    12181 agtcagcatt aacgaaagtc tatgatctgg aacgcctagc aggtcgagta gctttcggta
    12241 atgtcaatgg gcgagacttg atccagctga aaaattcttt agaacaagtg ccaatgatca
    12301 gagggttgat tgaaggaatc gatcaaggtc aatggcaaag tctgctttca gaaatgcagc
    12361 cgatggacca tctcgtccaa ttgatcgacc aagctatcca agatgatccg ccgttacaaa
    12421 taacggaagg aaacatcatc aaagatggat tcaatgagca gctcgatatg taccgttctg
    12481 ctatgagaaa cggaaagaaa tggttggcag aacttgaagc aaaagagcgt caagaaacag
    12541 gaatcaaaaa cttaaaaatc ggatttaacc gcgttttcgg ttattatata gaaatcacaa
    12601 aagcaaatct tggaaatgca gaactggaga agtatgaacg aaaacaaact ttagcaaatg
    12661 cagagcgttt catcacacca gaactaaaag aattggagac acaaatcctc gaagcagaag
    12721 aaaaatctgt tgatttagaa tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg
    12781 ctatccagcc gctacaagta cttgcaaaag caatcagtgc agtagatgtg ctacaaagtt
    12841 ttgcaacgat cagcgaacgc tatcagtatg ttcggccaga acttgtcagt gacaagcatc
    12901 agcttctgat caaagacgga cgacatcctg tagtagaaaa agtattagga catcaggaat
    12961 atatccctaa cagcgtagaa atggcagaag acgaaatgat cttgctgatt acaggaccga
    13021 atatgtctgg taaaagtacg tatatgcgac agttggcatt aactgttctg atggcgcaaa
    13081 tgggctgttt cgttccggca aaacaagcag ttttacctat ttttgatcgg atttttaccc
    13141 gaattggtgc aagtgacgac ttaattgctg gacaaagtac ttttatggta gagatgatgg
    13201 aagccaatca agccctgcgt tatgctacgc caaacagttt gattttgttt gacgaactag
    13261 ggcgaggaac agcaacctat gacggaatgg cgttggccca agcaatcatc gaatatatcc
    13321 atcgtcatgt acaagcaaaa acactctttt ctactcatta tcatgaactg acggtacttg
    13381 agaaagaact tccgcagctt aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag
    13441 ttgtcttctt gcataaaatg atggatggac ctgcggataa aagctacggc atacatgtgg
    13501 caaagatcgc aggattgcct tccaatctac tagaacgagc agcagatatc ctgcatacgc
    13561 tagaaaatgg cgataacgga caccatactg ttccagctga agtagaagag aaaactaaaa
    13621 cagccaaaca ggagcaaata aaaccacctg cagaaaaagt aaatcaagac catatacaag
    13681 accgtgtaaa agaagaaaac caacagcttt ctttattcag tgaattatcc gaaaatgaaa
    13741 cagaagtcat tgaatcactg aagcaaataa atttactaga aatgacgcca atggatgcac
    13801 taaacacatt atatgaatta caaaaacaac tgtaggaaga aggcggatat cgatggccaa
    13861 aatccaagaa ttatctgaac gtttagctaa tcagatcgct gcgggagaag tagtggagcg
    13921 accagcttcc gttgtgaaag aattagtgga aaatgcaatt gacgccggca gtactcagat
    13981 tgatatctta gttgaagaag cgggactgcg gacgatccaa gtgattgata acggagaagg
    14041 cattttggca gacgacgtag agaatgcctt caagcggcat gcgacaagta agatccataa
    14101 tcgcgacgat ctttttcgca tacgaacgct gggctttcga ggggaagctt tgccaagtat
    14161 tgcctctgtt tctgaaatga ctctggaaac ggcgaatcag gaagaaaaac aaggcaccta
    14221 tctgtcgctg aaaggcggag aagttattga gcatcgacca gcaccactgc gtcaaggaac
    14281 gaagattacc gtcagcaatc tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac
    14341 actgcacaca gaattagcga acatcgggga catcgtcaat cgactggcat tgagtcatcc
    14401 atcgattgct tttcgtttag tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg
    14461 cgatctgaaa caaacaattg cgggaatcta cggattatct accgcaaaaa aaatgctgaa
    14521 aatcgaagga aaagatctag attttgagtt atttggctat gtttcattgc ctgaggtgac
    14581 tcgtgccagt aggaattact tatcgacgat catcaatgga cgttttatca agaacttttc
    14641 cttgaataaa gcaatcgttg caggttatgg gtcaaaatta atggtcggtc gtttccctat
    14701 tgctgtattg gaaatcaaaa tggacccgct tttagtagat gtaaatgttc atccgacgaa
    14761 gcaggaagtc cggctgtcga aggaaaaaga actaacagag ctaattagta acgccattca
    14821 aaaagcattg tctcaagaaa atctgattcc aagtgcagcg gataatctac ggtttaagaa
    14881 aaaagtacct gatcagccta aaacagaaca aatcgaaatc gatttgtcct acgcttttga
    14941 agaagaaaaa cctaaaaagc caagtagttt gaattttgat cgagagacag gtaccttttt
    15001 cgtagaagaa ccaaagaaca catttgttga cagaattgtt gataagtctg tggaaaatgt
    15061 tgaaaactat tctggttttt ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac
    15121 ggtggataag tttggtgaaa atgttgataa ctttgtagaa aaaacagtgg aaaaatctga
    15181 aaagaaagaa tttcccgttg tggataacat ggagaaaaca aatgaagaac aagcagataa
    15241 atccgtagaa gaggatgaaa agatttacga ggaagaattg acactccatc ctgaatttga
    15301 tttcactcaa ggaaacgaca aaacattaga acaagccatc aagaaattag aaacggaaaa
    15361 accagtacaa cgttttcctc agttggaata ttttggacag atgcacggaa cgtatctttt
    15421 tgcccaaagt agcgatgggt tgtacatcat tgatcagcat gctgcccaag agcgcattaa
    15481 gtatgaatat ttccgggaaa aaattgggga agtatctgat gatctgcaag aattgcttgt
    15541 tccatttgtg ttagactatc caaacagtga tgctttgaaa ttaaaagagc aaaaacactt
    15601 gctagaagat gcagggattt atttagaaga atttggtcaa aacagcttta ttgtacgggc
    15661 ccatcctact tggtatccca atggagaaga agaatcgatt attcgggaga tgatcgatat
    15721 gctgctgaca actggaagtg tgagtgtaaa aaaattccgt gaagcaacag caatcatgat
    15781 gagttgcaaa cgatcgatta aagccaacca ttatttgaat gaagcacaag cacgtgtgct
    15841 gctgaaggat ctggcacgat gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt
    15901 gattcacttt accaattcgg atatggaaag gatgttcaaa cgaatccaag acccgcatta
    15961 agtcaaagag aagagggaaa acatgaagat tgtattagca tcccaatcac caagaagaaa
    16021 agagttattg gctcatatcg tttcagaatt cgagattgaa cctgctgata tcgatgaaac
    16081 gccatttcca gacgaacagc ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg
    16141 aattgtctgg gaacaagatg aaaaagaaga ccatttagtg attgccagtg acacgactgt
    16201 tgttctcaat caagagatca tgggtaaacc agaagatctg gctgaggctg aattcatgct
    16261 gaaaaaacta agcggggaaa cacatactgt atatacagct gttgttttaa aaacaaagga
    16321 acgagaagaa cggatactag cagaagcaca tgtcactttt tatcctttga cagacgaaga
    16381 gatcaaacgc tacttggaga cgggggatta tgcggataag gcaggggcat acggcatcca
    16441 aaatgctgcg agtgtattcg tcaaagaaat ttctggagat tattacagta tcgtaggatt
    16501 tccgattggt gctgtcaatc aggcactgaa aacattcatt taaacaaaaa agcggactat
    16561 ctgattttcg attgaaaatc catgatagcc cgtttttttt attgaaaaga ttacttatct
    16621 actctgacta a
//
LOCUS       DACEUF010000058        16543 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.061, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000058 DACEUF010000000
VERSION     DACEUF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.061"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            248..1123
                     /locus_tag="IXG20_001848"
     CDS             248..1123
                     /locus_tag="IXG20_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1610043.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(1275..2117)
                     /locus_tag="IXG20_001849"
     CDS             complement(1275..2117)
                     /locus_tag="IXG20_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1610044.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(2209..4086)
                     /locus_tag="IXG20_001850"
     CDS             complement(2209..4086)
                     /locus_tag="IXG20_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1610045.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(4083..5504)
                     /locus_tag="IXG20_001851"
     CDS             complement(4083..5504)
                     /locus_tag="IXG20_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1610046.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(5507..8896)
                     /locus_tag="IXG20_001852"
     CDS             complement(5507..8896)
                     /locus_tag="IXG20_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1610047.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            9153..10544
                     /locus_tag="IXG20_001853"
     CDS             9153..10544
                     /locus_tag="IXG20_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1610048.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(10617..11264)
                     /locus_tag="IXG20_001854"
     CDS             complement(10617..11264)
                     /locus_tag="IXG20_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1610049.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(11272..11958)
                     /locus_tag="IXG20_001855"
     CDS             complement(11272..11958)
                     /locus_tag="IXG20_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1610050.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(11964..13037)
                     /locus_tag="IXG20_001856"
     CDS             complement(11964..13037)
                     /locus_tag="IXG20_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1610051.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            13259..13933
                     /locus_tag="IXG20_001857"
     CDS             13259..13933
                     /locus_tag="IXG20_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1610052.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            13938..15278
                     /locus_tag="IXG20_001858"
     CDS             13938..15278
                     /locus_tag="IXG20_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1610053.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(15427..16413)
                     /locus_tag="IXG20_001859"
     CDS             complement(15427..16413)
                     /locus_tag="IXG20_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ1610054.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         4725 a         3094 c         3037 g         5687 t
ORIGIN      
        1 tttgtactct ttccattgcc aagataagcg attgtaagca tgagtggtgc ctccttttat
       61 tgatgttttc agtatatcct agaaaagcgc agaaagtatt tataatagct attctggtaa
      121 aaaagttcag aaaaacactt gttatcattc ggtttggtga aattaatgtt caaagtaagg
      181 ataaaaatca aaaagatagt agcggaacca agtgtttatc agtaagatag aatttggagg
      241 tcagatcatg cttattgaag aaaaactaac aaaacaagag ttgtttacca cgacagagaa
      301 acggattgtc gattacatcc gtaggaatat cgaagctgca gtctacatga cgattgaaga
      361 attagcaaaa gctacgtata cttcccattc agctatcatt cgtttatgca aaaaaatggg
      421 attcagcgga tttaaagaat tccgtttcga gctggggcgg gaagttcatc aattgattgc
      481 tcagttcaat caaacgattg atcctaattt tcctttttca agtgaagata gcactcagca
      541 aattgcaaag aaaatggcgg atttatcgat ccagtcaata aaaaaagcac aattgcagat
      601 tgaacatgaa gatttagatc aaatcggtaa agtattgaac aaggcacagc gaatctttct
      661 ttttgcaaaa ggtgattctc aaatcacggc aaggaaattc cagaataaaa tggtcaagtt
      721 gaataagttt ttgatcatgg ctgaagaata cagtgattct tcttggaatg cagccaattt
      781 gactagcgag gattgcgcca tttttattag ctacagcggt cgcatccatc attatgaacg
      841 aataatgacc tatttgaagc atgtagaggc ccctaccttg ctgattaccg gaaatcagca
      901 ttcagaaatg gccaaacagg ctgatatgtg tcttgttata tcgcaggagg aatatgattt
      961 tgctaaagta gcgactttct cctctcaaat tgccttcgat tacgtattaa atacgttatt
     1021 ctcagtgata tattctcaaa attttgaaga gaatatccgc aacttgaaaa acaaacaaga
     1081 attgatgcaa aaaggccttc ttaaagaata tcggaaaact taagaaaaga tcctcattta
     1141 gtaataagaa agatagtgtt tgataggctc tctttgatat catcataaaa acaggctgaa
     1201 gttttctttt tcaaacctca gcctgtttgt ttacggaacg attcgaatag atatattata
     1261 gaaatgagga atagctaact tctgtttctt attttgaacg attgacgaaa aagatatata
     1321 agaaggagga aaatcagtat acatgcagct aacaatccat acaggaaaaa tcgtttgttc
     1381 tcggatactt tctgtacttc tttttgaact tcttttgcat gataaggaac ccggtgccct
     1441 ctgacaagta agcgatgact gttgaccata taaggtgtac aagttaggag tgtcacgaaa
     1501 tcctgccctt tttcgattct cagatctttt gtatcagtcg gttctaccgt cttgatctga
     1561 tcgacttgat aagcgagtgt ccgtccattg atttcgatat agaaagaatc acctaaagtc
     1621 agtttcggta agtctgtgaa caactttgct tgaggcagtc ctcgatggct ggaaataacg
     1681 gcatgtgtat tttctccccc cgtaggataa gacgttcctt ccaataatga ggcacctttt
     1741 tctagcagca cagtattcgt ttcatcaaaa attggcaaat ggacattgat ctcagggatc
     1801 gtcaaaatac cgatcgtatg tttctcaaaa taggttctat cctttttcgc aggttctttt
     1861 ttcttagtaa agggatctgc acctggattt ccaccttctt ttgctaattg ctggtttttc
     1921 tttgtcatgt tttcttggat tttagccatg acttcttcat tttctttgac cgcttgttgc
     1981 tgataatgag agatgatctg ctgatccaga taattattca gcgcgtcgct aacaaatgga
     2041 taagctagta tccctattcc tgagagaagc aagaaaagca tcagcagatc tagaagtttc
     2101 cttttctttg gtttcatttg tgttctcctt gtgatgactg tttgataata aaagaaaaag
     2161 gagcatttgc gtaatccgct gctccttttt ccaattcttg caaaaagact aaatctgact
     2221 gtgcttgcgt ctagcaaagt acagtccagc aatcagtaga aggactactc ctgcaccgat
     2281 atacacatag attcccttac cgcctgttga aggaagtgta cctttgtgtt tatttggtat
     2341 tttttcagga gaaatcaact gtcctgctgt tacataagat tgttcatcga tagtaaatgc
     2401 tacacggttt gttaatggaa catatttttc tggcgctttc gtttcttcca gatagtacgt
     2461 gccatatttt agacctgtca catcgattaa accgttactt gtggttgtaa aaaccgttgc
     2521 tgattctttc gccgataccc agctgacggc ttttgtggat gggtcgatcg ataaatattt
     2581 cgctgtgtca ctatcttgat cacgaacgac gaattctgct ccagcaagtg tttggtctga
     2641 tgtaacgtca ccatctactt taacaaatcg tttgccccca gtaacgacat cgactgacgg
     2701 tggtgtttga tcatttgtat gcccgttatc gacatttgct tggttagaaa atcctttggt
     2761 tggatctgct ttttcgttca aatgcatata gtaaacgaat ttcaatgtac cgccaggagt
     2821 taatgaagga atataattcg gatcaactga aaccgtgaat ccgtctgttt gctcagtgac
     2881 agaataattt actgggtcga tttctttatt tccatcatat aaggcataag catacgttcc
     2941 tgaagaatca ttatcaaatg ttaaagcagc gtcatgagta tcgataagtt tgaatgttgt
     3001 gtacttgttt tgcgttcctt ctttatcagc gatacctaat gggatattga cagaaatctc
     3061 atattgaatt ttttcaccga ttgcgacatc ttttccattc aattgaggtg ttgttttatc
     3121 aactttagac gtatcatttt tgatggtctt ttctactggt gtttggctat ttgccaggat
     3181 ctcaaaaggc gtgattgttt gcttttcaat catttccgca ttatcaggag cttttacttc
     3241 ttctaaatta tattttccaa cttctaaatg attaacgatc aactggcctt tttcaataga
     3301 tgcctcggca aagtcattgt tgccgatgtc ataagaatga ccagtaatga aatgtttggc
     3361 tttggtttga tcagttgtcc aagtgtacaa tccatctgtg acactttgga tgtatttttt
     3421 gacgcttggt gttccttctt ctttagaaat aataaattct gctccattta gtgcttcgtt
     3481 ttcggcagta ccgatttttg taactttcaa cgttccatca ttaccgactg tatttttagg
     3541 gtagagatgg atagtatcta gttcttctgt cccgtattta taagagccat ctgcttgttt
     3601 gatcatctca tatacaggga aagctaaaac catgtttgcg gcagctgaca ctccgtcttt
     3661 tggttcttct ttgatcgtgt agactgcatc tttcccattt tgttttttag gtaaagataa
     3721 agtgacattt ccatcagcat ctgtcgttcc tgaagcaact ggtgtaccag gagtcaaaga
     3781 ctggactgct tgttttgctg catccacgga cgctccttta tctcgttgcg cataaaattc
     3841 ttgagtgacg ttataaactg aaaatgaaat atcggctaat ccttggtatt gatcgaattc
     3901 gctcatttct ttcccgctgt tttggattaa aggatcgggt aaatcagtca ttttcttttt
     3961 atgaaggatg acttgagctg ctgtctcctc tgcaaacact ttttttgcgc cgaagctgtt
     4021 tgtgagtaat ggtaaaataa ggaaaaggac tcctaacata acgtttattt ttttatgatt
     4081 tttcattttt tcactcctgt tctttttttg ataaacaacc agattgcaaa gcttgcgaat
     4141 accataccaa caacgcctaa aagtgtattt ttcgtttcgt ttgtttttgg aagaaatgga
     4201 cggctatttt cttttttatt gactactttc aatatactgt ccgcctcttt atcctcagaa
     4261 agaaaagttg aaatggtaaa cggaatttct gtttcactta aaatataacc agaaggtgct
     4321 tcgatctctt ttaatcgata acttcctgtt ttcaacccgg aaattgaaaa agcgccattt
     4381 ttatcggaaa tcaggtgaag cgcatctgat tcgttctttg tccacttgta tccgttggct
     4441 gtttgctgga ggtaatttcc tgctttgttt ttaataagga atactgcgcc agctaatcgt
     4501 ttatctggtt tatttgtctc gattttgaca aattgcttac tccctgtata tacagagact
     4561 ttctttgtac tgacaccaaa accaaaatcc agtgttccgt tgttgttgat ttccttgttc
     4621 gcctgtgctg tactgcttag ctgcatctga taagaaatgg tcagtttttt tccagcatat
     4681 ttttccaacg aagggatcga aaataaaaca tcaaatccgt gaggtttctt gtgtatgaca
     4741 aagccttctg tcagcttttc tccattcgat gaaatcgtta aactattagg taaaaacgtt
     4801 aatgcttcat ccgcactatc tgacaattcg aacttttgat agtcaaggat atttacagga
     4861 attgtcgtat gcaaggaata agtgattttt tcaccaatcg taaaatcatt tctaggctct
     4921 tctaatacct tctcaaaagg tggatcaggg tagtcgtttt cttcattttt aggatacaaa
     4981 tggatagttg aaagtttttg attgttttgt ccatgtacag gaagaacaac taccatattc
     5041 tctgcctttt ctttgacgac ttctggtact ttggattcaa tgaaaagata cgctttatct
     5101 cttttttctt gatctttagc ggccagagaa aaagaagcga taccatcttc gttgttaaga
     5161 gttgtagttg ttgcttctgc ggtaaacata ccggaagacg caccgttttt tgcgatctct
     5221 gcttgtgctt cttctaccgt tttgcccttt tcccgtagat ggtaaaaaga atctgtgaca
     5281 tcataaactt ggaatgtgac accatttaat cctcgatacg tttgtaataa agctttttct
     5341 tgtccgtcat ttggatgatt tttcggcagt tgcccgtttg ggaaaagcaa tttgtgtaaa
     5401 gtaatctgaa ccatttctgt ttctgtttct gttgctacct gagtaaaagc tggtttaaat
     5461 agtaacaaga agagacacat actaagccaa ccaagttttt tcatatttag ctgccccctc
     5521 tttctttttt caaaaaaaga taccaaatag aaaacgcaca accaatcatc cctactagat
     5581 agattcctaa acggccaatt cctcccgttt caggtaatgg tacttttgcc tgattcgtga
     5641 tgtctaaaga aatctggttg tttttggctc ctggtgacag aacagattca tgatcttgtc
     5701 catccacttg aattgaccca tcttcgtgta taactatagt aactggctct tttagacctt
     5761 ggtatccttc tggtgtaaaa gtttcggtca gcgtataagt tccaggtttt aaattttcga
     5821 atagaaaggt atctgtttct tgtccatctt ttggcgattc taagtccatt tctggtccct
     5881 gcagtctgaa ttttgctcct tttaatgtct gtccgttgtc ttcttttttg tgtaccgtta
     5941 gatcaaacgc ttttagtcga ttttgatgag tgagcgtcca tacattattt gtcatctcgg
     6001 tattttttcc cgtgtaaatg atttttccat atttatcgat ttggaattca taatttcccg
     6061 gcgaagtatc atatccggca ggaccagctg tttcaacgac tcgatattta ccaggtgtga
     6121 gtgaatcaaa actggcaaga cctttttcat tcgttgtttg gctgtcaatt ggctgataag
     6181 taccatttgc ttcttttttc tgtagggaaa atgctgctcc tttaagattt atctctttgt
     6241 ccgtcccttg catcgcatac ttacgtactg ctacaggaac ttcaacaaaa ggattttcaa
     6301 tagtcaactg gatcgtatca tcggaagtag tgactgtttt tccatcaatg gttaccgtac
     6361 catcagaagc gatcttgatt tcccaagtag tctttttgct taatccatgc ccttctggag
     6421 cttttgtttc tgtcagcgta taggtcattt ctttttgcaa tttgacattt tctggaagag
     6481 aataggttcc gtcgccatga tctgttagta aagtatcaat agaatctcct gttaatttga
     6541 aaacagcgcc aataagatcc ttttcacctg tagaggaatt tttcgttatt ttcaagttta
     6601 acggtgtgaa ttgtttagta tttttccatg tcattgcatc tatttgttga gaatcgtatc
     6661 ctggtacagg taattctttg attgtttggt aactgaatgc ttgaccttga ttattgtatt
     6721 gaggtagtga taatatctct tgataacttt ctccgctatt tgcagataat ttgtcaatgt
     6781 ctgcacgttc ccacgtattt gttgtatctt tagctggttt aatgattcga atatatccgt
     6841 ttttccaagc tgcagcattt gtcgtatgtt cccgttgaat ctcaaaagta acttgatctg
     6901 gacgatcagc tagattgttg tcaaattctt cccataactt tttgatgtga agactgactc
     6961 cgggagcttt agcagatggt ataccgaatt cagctaattc attggtatca atgtttggct
     7021 ggaaagttgt cctgccgttc atttgatacc agaaatttgg atggaagtcc tcattttctg
     7081 tttggattct cacttggtaa tggatttgga tttcttggtc ttttcctaaa tagatctgat
     7141 tgctcttgat ggtatttcct tctatggaaa tagatggagt gaccgttgta ggacttgtcc
     7201 ccacactctt gactgaaagt gtaccaggct gataaatgaa tggttctgcg attggatcat
     7261 ttatttgtcc attgcttaca gttgctgaga tctgtacagc ttttttggct aggtattcag
     7321 agatatctgt tgcatgatca gctgattcat agtaaagatc gcctttttca tctgatgaaa
     7381 ccatttgtcg catacgagac tctacttctg cttttgagag accagctgcc ggatcgcttt
     7441 gaagttggat gccaagacca tgtatttcga ttcctcgttc tttgagtgcc atcgcttctc
     7501 cgatggttgc gataaacgta ctgtcgattc ttctggatag attgttttgg tcaggtgcat
     7561 catagattct tgagattaga gaagtatttc ccggccgatc ttgcgtatta gaaaactgag
     7621 ttccgtaata attgctgctt gattgtgcgt gtacccgctg tactttatag gaaaatgttg
     7681 gtacaccatc cgtcagcaaa acgatcactt ttttatgtcc atttggaacg gatagcatgc
     7741 ttcctgcatc tcttagtgct ttttgtgtaa aagtaccacc atttgtccgt gaaggtgtaa
     7801 tggattttac ttgatttttc actgaatcaa atgaacccat ctgtactgca ccgttactgt
     7861 agctatatcc ttcgcttgag tagccgacat atcccatatt gattttgtct gtgataccgc
     7921 tatctgctaa agtatcgaca aaacgatcga caccaatctt tacttcaccg atccgattat
     7981 tgtcgttcat acttcctgac cagtctacga ccaatacgag atcaagaggc gtgatatctt
     8041 tttgtacatt tccacgagca ttcaaataaa catcaaacaa tccgggtgta cttgtttcct
     8101 ttgcatactt tcgtatcgca tagtctgctt gattaccggt tcctccatat tcgatatacg
     8161 aatgggtccg atcattagga tctccattcc aagaatttat accatcccat tgatttgttc
     8221 cgtttttatt gccttgatga tcaagaacat ttgtatttcc tgtaggttgc caactggctt
     8281 taggataagt gccctgatca tctgttgtat attctgtagg aatcgtttga ttagatggca
     8341 cactcacagc tggcaaggat aatgtaggca atgtaaatgg gctgacagaa ttttcgtttt
     8401 cttttgatga ttcttcttct atttcgtctg gctttggtac tgacacatct gtatctttct
     8461 tattttctgt ctcatcaata atgtcatatt ctttattatc gacgatcagc ttgtttgcct
     8521 ggctgtggta aagtgctgga tcgattggaa aactcagatc gatagtttgt ttagtgtttg
     8581 atgagatttc caacgtaatt ttcccgctat ttacctgata agcatagcca tctccttctt
     8641 ttctttccac atcacttaag taattttcga ctagaccaat ttctttttct atcttttcag
     8701 cactggtgtt attcgcattt agcttgatgt tcagtgcttt ttttccagac tgatccgaaa
     8761 tgacttcagc tgaaattgtt tctaaaactg ttggatcatc tgtagtgtct gctattgcag
     8821 ataatggcac aaagtttgat aaactcaata ttatcagcat gaaaataact ttcagtttct
     8881 tccctgtggt cgtcattttt cccctccttt ttcttttttt ctgttttgac gtgaatagtg
     8941 aaaattatta aaataaacag gcgttacata attttattta ttttaatatg ataattatta
     9001 tacgaagggc gctaaaggga atcacaccaa ttttcatacc aattggtttc atttatgaag
     9061 ttgattcctt cgattaatga gcgtttcatt gaaagaaaag ggttgaggga gaaaaacata
     9121 tgatcaggtt gttggaatta aactatagaa agatggaaaa aatcattcga attctgtcca
     9181 tttctgacaa gtggatgact aaaaaagaac ttgccaaacg aattggtagt tcagaatcta
     9241 catttgtaag gtatatcgaa gagatcaaaa tccgctgggg aacggatgta acgattcaat
     9301 cttcacaaaa attggggtat cgtcttgaac atttgaatgt accagcttat ttaaatgttc
     9361 tgatggaaat gtcgcaaagt tctacaaaca gtcgtttatt aaatgagatt attgtcaatc
     9421 cgggtaaaac gattgagtat tattgtgaaa aaatttccat cagccggtct tcttttacac
     9481 gtaagttgaa acaatgcaat caaattttag aaagctattt attaaaagtg atcgttgacc
     9541 aaggttttca gttaactagc cttaaaagtg agctccagtt gcgtatcttt gttacctttt
     9601 tctttttaag ttactacgga agacgcacgt tgccatttcc aatcgaaaaa aagaagatca
     9661 aagaaattat ggtcaagaat aattgtcaga tgaatttgtt tcaggaagaa agcccatatg
     9721 agcaatcttt ctttattatg tattttgttg tgcatctcat gagagaatcc caaggaactc
     9781 catttgaatg gaaagctgga gactcttatg tacagttgat aaatatcgat aaaaaagact
     9841 tcttaatctt gaaaaatctc ttaggaaaat tgaagtacag cagctatata aaagtagtga
     9901 actattttgt caatacgatt ttttcagaat tttcggagaa agaaaaaaat gaaatcagtg
     9961 agcagatacg aaaaaatatg tcctactgtt cgttttcatc cgctgattgc aaagaaaaaa
    10021 acgaagaata tgttgttcaa ttattaacgc aaatttattt tttctcccgt attttcccat
    10081 acgatacgac tcatttgacg atccgtgcaa cttactttgc acagcagatg caacaccgcc
    10141 agatacgtct atatgctgga tttcgtgcag aacttcaatt ttattccact ctactgaatc
    10201 agaatctgat ttattattta tctagctata tcttttggat gcttatcggt aatcctgaaa
    10261 ttcaccatta tacttctgat aaaaaaatac tgattatcag cgacttaagt cttcgacata
    10321 gtcaatatat tgaagaatat atcagtgata ttcttgctgt gcataaaatt cactctgaga
    10381 caactgctat tactgaagat cagctgtcga aatacaatct tctcgagtat gatctgatcg
    10441 ttacgaatca acccattttg aatgcccatg ttcctcatat attgattgat gatagtgtgt
    10501 cattcgcaaa tgaagaagaa ctcatccgct tattcgagtt atgaataatc aaaaaacagc
    10561 tatgtataaa ccaccctgat aaaaatttca ggacaaatac acatagctgt ttctttttat
    10621 tgtattcctt cagcaatcca acgagattta tagcgaaagt cttttaacaa ctcttctttt
    10681 gtccaattat ttgcaatgag atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat
    10741 aggaaactaa ctgcttcttc cgacgattga atggagaaat gattgattct taataattcc
    10801 agccattttt cgttcattgg agaatcagcc gatagttctc tacgtttggt tgtctgctga
    10861 tcatttcgct gggttaacaa gtataacctt tgccaataga tactttcgtg atctaagtcg
    10921 cagcaccact ctaaatattt ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg
    10981 aattttaaga tatcttgatg gactatgtga gaactgcttt tgatcgtata caaataagca
    11041 tcttccaaat cttgaaaata tttataaaaa gcaccgcgcg acatccccat cttgtcaacg
    11101 atttctgaga ctttgacttg gctgatgtgc tgtgagcaaa atatctccag taataggtca
    11161 tcgattctct gtttctttgc gttcgataaa ttgaaaaaag tttcttttgg catccacatc
    11221 tctcctttat ctctgattat acgtggatag cccttcattg tcatgaagga ttcattcctt
    11281 ctttttctct tcttctgtca agtacccatc ttccattcga taaacgcgat cactccattg
    11341 aatcatacgg acatcatgag taaccataat agtcgctttt tgtttctcat gagcttcttt
    11401 aactagtaat ttgacgactt catatgcatg atctgtatcc aaactagctg ttggttcgtc
    11461 tgctaagatc aagcttggct cattatataa agcgcgagca attgccactc tctgtctttc
    11521 tccaccagaa aggtctttag gataactatt tttaagtgac tggatatcta aagattgcag
    11581 caaggaatcg atttttgttg aatcttcttt ttttgaagta acttggtcaa ctagttcaaa
    11641 ttgttccttt accgttaaaa aaggaatcaa attagagctt tggagaataa atcctatttc
    11701 tttaaagcgt aaatctgctc gttttttttc tggaagctca gttagtgagt gaccgttgat
    11761 cactatttct ccggaagtcg gtgtttgcaa tcctcctgaa atggtaagga aagtactttt
    11821 acctgaacca gaaggaccaa tgatgctgac gaattctcct tcctttacat aaaaatcaat
    11881 tgctttcaaa gctgagacct ttgtatgacc agaaccaaat gttttggtaa cttttttcat
    11941 cgttaatatt tttttcattt ttattaacct cctattgctg taattgggtc tactttggtt
    12001 atcgtacgta tcgaaaacaa tccgcctagc attgcgacaa tgatcaaaat cccactatat
    12061 agcaaccatt gtgaccaaaa aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc
    12121 aaataagcaa acaaaagagc aagcagaacg cctaccaccc caacaataaa ggactgtgct
    12181 actaaagatt tagcgatgaa gaaatttttg atcccttgtg ctttcattac tccaaaaata
    12241 gcagtcttct gaagtgtcat cacgtacatg aaaataccga ccacggcagc aaccactaag
    12301 aacaaaaagt atatcatcgc atttaaggtc atattttggg cagaatagcc aggaatactt
    12361 tcaatgaatg tcccaatagt taataactgt agacccttcg atgctttttc tttcgaaatt
    12421 ttagctgaat cctttattac gatcccattg atcggctgtt cttcttcaga agcaaacggc
    12481 tgttgtccat acttcaaagc agtccacgta tctagggacg catagatgac gggagaaact
    12541 gtgtaataag tttcaggaaa gataccgaca atcgtcaaag gatcgtcgta actgccaatt
    12601 ttgatcgtat ctccaatttt atagcctgaa tcagccagat tttgagagat gatgatttca
    12661 ttctttgttt taaattcatg ccctttcgtg agttttggta ataaaaatgc ttctttcgtt
    12721 gtgccgaaaa ctgtaacatt ttctttgttt tttcctttga ttgctccgct gtacaatccg
    12781 attggtgctt tctctcctcc ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct
    12841 gcaaatactt gatttgcatc ctccgacaaa acaatctctt gtgcgtccca atcatcgatt
    12901 gcttttttaa actcttctga aagtccattc gctagtcctg acaacatgaa tacgacatac
    12961 gcaatcaaga acataatacc gacgatcaag ccatatcgta atttagaata gcgaatttct
    13021 ttgatagcta gaaacatttg tttttcctcc ctatgataga ttgatagtga caaaatgtca
    13081 cttgttgaat agtgtaactc taaagtgaca atttgtcact tgtgttgttt ttttcttatc
    13141 aataaattgt ctaagacatc tttcgataaa accttaacat gagtcataaa aaatttattt
    13201 aagccttatt tatgctttcc atgataaact aaagtgacca aatcaatgag gtgggaaaat
    13261 gattaaacta ttagtagtag aagacgataa agtattatcc aataatataa aagaaatact
    13321 cgctgcaatc ggcgagataa cacaagttta tgatggtagt gaaggactgt tcgagttgga
    13381 aagtggagtt tatgatcttg caatcttgga tttgatgatg ccggaaatga atggatatga
    13441 agtgttagca aaaatacgga aagaaaaaat acagactcca gtattgattt tgactgcaaa
    13501 agatagtttg gaagataaga ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa
    13561 acctttctat cgagaagaac tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg
    13621 tctctttgat gaaaatgttc tgaagtataa ggatatcagt tgtgatttaa aaagcaatga
    13681 agtcttgtat aaagaacaag ttttgcccat acaaggaaaa gagtttgaat tgctcgttta
    13741 ttttttacaa aataagggaa tcattttgac aaaagagcag atatttgacc ggatctgggg
    13801 atttgattct gatacgacga ttacagttat tgaagtttat atgagcaact tacgaaaaca
    13861 tctgcgaagt actggattgg ataaagaaat caaaacctta cgtaatgtcg gttatatact
    13921 gcaaagggtg tgatgcaatg aatggagata ttacacgaaa gcaacgtagt cgcttttttg
    13981 cgacaaatgt agcagcattt gcttttatct tttttctttt gggattcatt actttacaga
    14041 tattaaacca atctgcatac agagaaacag atctgtcatt agagaaaata aattttgatt
    14101 ctcgaacagt acaattagag atacaaagat atcagcaagg cgatcccttt ttaaacccga
    14161 ctcaaacgga acaaaacaga acaaacatgg aacagccatt aatggaacca tcaaatgacc
    14221 gttttaacac tcaagtgatc ttgtggtcta aaaatggtga gatattgaat aaagcagcta
    14281 ttggtgggcg tttcaaccaa atcaaagaat taaaaatgga tacgaaaaat ctgaatacca
    14341 tccaaaatat tgaattaaat gaagacagtg gctcatctgc cctctctttc cgttcgatta
    14401 cgaaaaaagc ggagacgaat caagatggcg ttgcctacat ccaaatttta gaaaatacaa
    14461 atcaaatcaa gaattcatta gcgacttttc gcacgattat gattttgtgt atgattgttt
    14521 tttggattct ctctattggt atcagttatt acttgtctag tctaagcatg cgccctatta
    14581 ttctttcttg gagaagacaa aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac
    14641 cgttaacgat tattcaaaat agtttggaac atctttttac caagcctaat catactatta
    14701 ttgaagaatc cgaaagcatc gctcaagcat tgtcagagac gaggcgcctc actggattga
    14761 cttcggattt gctgacaatc gctcggaatg attctgatca gcagctgctt tcaaagcaaa
    14821 tgatcaatac acaagaatat atcgaacaac ttgttaaacc atttcaagag atggccatca
    14881 tagatggaaa agagtttatt ctccataacg atgcaacatt agaagtacca attgatgaaa
    14941 aaaaaatcca tcaagttttg gttattttgc tggataatgc attaaaatat acgaatccag
    15001 aagataagat cattttggat tcatctgtta accataaaga atggctgatt cacgtaaaga
    15061 atactggccc ttctatcagt gaagaagatc aagcaaggat ttttgatcga ttttatcggg
    15121 aagatcaatc tcgggcgaaa gagactggcg gatacgggct tggcttggca attgcaaaac
    15181 aggtaatcga acagcatcat ggacagattt ctgtatccaa ttatcttcca caaggagtcg
    15241 attttaaagt ccaattgccg ttgaagccta aaaaataatg cttatcccta aaaaagggct
    15301 gtgacaaaaa tcttgtcaca gccctttttt gttgcgaata aacggtgttc cagaagtagc
    15361 ttcttcggaa ataagccgaa atttctttaa gctgaccact tctgtcacaa cctctttagc
    15421 ttacgcttac gattttttat tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc
    15481 caattcttct cggctaacat cttctgtcac tttttcaaca tcttcatgtt cttctttatg
    15541 aatcccaact tcttcacgaa taactgtatg tttgcttaca tctacttgct cttctttgat
    15601 tgggatagta atcgtgtcat cttccaattt ttcgtctgtt ccctgtgaag attgatctgt
    15661 tactggtttg cgttcgatga cgatttcttc acgtttgacc ggtacttcta ccgtttcagt
    15721 atcgttgacc acatgtttgt ggatgtcgac ttctccagtt gtcacatcat gtgtatttac
    15781 atctaatttt tcttctttta gttgaattgt atcatcatct agatttggtt tgcgatcgat
    15841 gtcttcagtc ttgcttgtac ccattgtatc agtctcgcca ggtacgtcca tcggatcagt
    15901 tcctggtgtc atattcaatg gatccgttgt attttgaaag cctgtcgtgc cacctacgtc
    15961 cattggatct gttccagtgc cagtttctaa tccagtcccc attgtgcttg ctgggctaac
    16021 agtatcttgt gtatccattg tatcttctgt tgtttctggc tgacgatagc cttttactgt
    16081 gatgacaaat tttccatcag aaatatctcc tctatattga tataaaggat cattttctgg
    16141 tgtagccgtc tcagaatcat aggtatcaaa agaaaaagta tcttttattt tttcccataa
    16201 agaacggtct tcttcaccat ccatccgatt ttcatctgtt tcaacatcta taccagacaa
    16261 tccgtcaaat cctagtctag atgctgcttc atcagtagta taaataacga tatcatctcg
    16321 ttcataccct tcgttacgca accgttcaat tactgataat gcttcttccc tcgtactata
    16381 gcttccgatg accgttaaat ttttctcttc catgtaaatt cctcctagca aatagattta
    16441 tgattggtat attgttactt ataaatttat tctataggag acgattgttt tctacaaata
    16501 tttagattgg aatttgatta aaacaattta attgaaattt gaa
//
LOCUS       DACEUF010000059        15900 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.062, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000059 DACEUF010000000
VERSION     DACEUF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.062"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(52..522)
                     /locus_tag="IXG20_001860"
     CDS             complement(52..522)
                     /locus_tag="IXG20_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ1610055.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="IXG20_001861"
     CDS             complement(681..1139)
                     /locus_tag="IXG20_001861"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ1610056.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="IXG20_001862"
     CDS             complement(1310..2455)
                     /locus_tag="IXG20_001862"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ1610057.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="IXG20_001863"
     CDS             complement(2468..3385)
                     /locus_tag="IXG20_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ1610058.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPNGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="IXG20_001864"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="IXG20_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ1610059.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4862..6121
                     /gene="lysA"
                     /locus_tag="IXG20_001865"
     CDS             4862..6121
                     /gene="lysA"
                     /locus_tag="IXG20_001865"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ1610060.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6108..6908
                     /gene="dapF"
                     /locus_tag="IXG20_001866"
     CDS             6108..6908
                     /gene="dapF"
                     /locus_tag="IXG20_001866"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ1610061.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6911..7792
                     /locus_tag="IXG20_001867"
     CDS             6911..7792
                     /locus_tag="IXG20_001867"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ1610062.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7789..8505
                     /locus_tag="IXG20_001868"
     CDS             7789..8505
                     /locus_tag="IXG20_001868"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ1610063.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8511..9206
                     /gene="dapD"
                     /locus_tag="IXG20_001869"
     CDS             8511..9206
                     /gene="dapD"
                     /locus_tag="IXG20_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ1610064.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9222..10223
                     /locus_tag="IXG20_001870"
     CDS             9222..10223
                     /locus_tag="IXG20_001870"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ1610065.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10220..11581
                     /locus_tag="IXG20_001871"
     CDS             10220..11581
                     /locus_tag="IXG20_001871"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ1610066.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11541..12692
                     /locus_tag="IXG20_001872"
     CDS             11541..12692
                     /locus_tag="IXG20_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ1610067.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12667..13836
                     /locus_tag="IXG20_001873"
     CDS             12667..13836
                     /locus_tag="IXG20_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1610068.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14011..15390
                     /locus_tag="IXG20_001874"
     CDS             14011..15390
                     /locus_tag="IXG20_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1610069.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15671..15900
                     /locus_tag="IXG20_001875"
                     /pseudo
     CDS             15671..15900
                     /locus_tag="IXG20_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5203 a         2802 c         3384 g         4511 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aaccattagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaaa
     4741 tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc aataaaattc
     4801 aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa
     4861 aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac
     4921 acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg
     4981 ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat
     5041 tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga
     5101 aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa
     5161 caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga
     5221 taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatttg
     5281 tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc
     5341 gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca
     5401 gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca
     5461 aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga
     5521 ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa
     5581 tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc
     5641 tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt
     5701 tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa
     5761 atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga
     5821 atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc
     5881 aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga
     5941 atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa
     6001 ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat
     6061 tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata
     6121 aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac acagattata
     6181 gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tatagacgga ttgatcgccg
     6241 taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt
     6301 gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac
     6361 aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac
     6421 ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta
     6481 atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca
     6541 ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag
     6601 tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg
     6661 tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag
     6721 taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa
     6781 ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct
     6841 ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg
     6901 aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg
     6961 atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga
     7021 tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga
     7081 gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc
     7141 tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc
     7201 agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc
     7261 tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc
     7321 tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat
     7381 cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat
     7441 tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt
     7501 aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt
     7561 agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact
     7621 tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc
     7681 attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa
     7741 aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga
     7801 ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat
     7861 acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg
     7921 ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc
     7981 ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa
     8041 gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc
     8101 atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc
     8161 gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg
     8221 ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa
     8281 aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa
     8341 catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct
     8401 aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca
     8461 ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc
     8521 aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga
     8581 atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg
     8641 ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca
     8701 ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa
     8761 aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca
     8821 atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga
     8881 tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg
     8941 gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag
     9001 aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag
     9061 atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg
     9121 gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat
     9181 tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct
     9241 gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg
     9301 attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg
     9361 tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg
     9421 atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa
     9481 gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat
     9541 taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa
     9601 attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc
     9661 agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag
     9721 atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa
     9781 cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa
     9841 tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg
     9901 tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt
     9961 ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg
    10021 atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg
    10081 ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg
    10141 ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga
    10201 aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc
    10261 gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg
    10321 ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga
    10381 cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa
    10441 aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga
    10501 agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt
    10561 agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt
    10621 tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac
    10681 agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta
    10741 cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg
    10801 cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg
    10861 aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt
    10921 tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt
    10981 cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca
    11041 agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg
    11101 acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt
    11161 tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc
    11221 agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct
    11281 catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt
    11341 agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt
    11401 gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga
    11461 gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta
    11521 tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg
    11581 aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg
    11641 aggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag
    11701 aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc
    11761 tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg
    11821 cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt atttataatg
    11881 tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt
    11941 caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac
    12001 tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag
    12061 acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc
    12121 tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag
    12181 agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag
    12241 ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt
    12301 tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact atttcttttc
    12361 gctataatga cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag
    12421 attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat
    12481 atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac
    12541 atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa
    12601 cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag
    12661 taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca
    12721 aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg
    12781 ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc agcgattgaa
    12841 gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt
    12901 atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca
    12961 gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag
    13021 gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct
    13081 gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa
    13141 cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat
    13201 cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa
    13261 tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat
    13321 atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca
    13381 catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa
    13441 ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg
    13501 gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca
    13561 cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac
    13621 ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt
    13681 tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca
    13741 gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc
    13801 tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca
    13861 atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat
    13921 gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat
    13981 tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt
    14041 gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta
    14101 gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg
    14161 tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt
    14221 ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa
    14281 aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa
    14341 ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa
    14401 aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat
    14461 ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa
    14521 gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc
    14581 acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt
    14641 gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc
    14701 aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca
    14761 aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat
    14821 gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat
    14881 tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca
    14941 cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta
    15001 ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt
    15061 acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca
    15121 aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca
    15181 atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga
    15241 aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac
    15301 ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa
    15361 gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga
    15421 aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
    15481 aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat tttcaaaaaa
    15541 gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt
    15601 ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag atcgttcatg
    15661 attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga ttgaagcgga
    15721 aatgcttaaa attcgggaga agcacaagga ttatggctgt ttacggatga ccaatgaact
    15781 aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcttgatca agaagctagg
    15841 aataaaagta acggcataca cgcgtaaatc acgtcgctac aattcttatc gaggaaaagt
//
LOCUS       DACEUF010000060        15825 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.063, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000060 DACEUF010000000
VERSION     DACEUF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.063"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(266..466)
                     /locus_tag="IXG20_001876"
     CDS             complement(266..466)
                     /locus_tag="IXG20_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1610070.1"
                     /translation="MKINYTVDLVSFSLVINFCLFSLRLFIEGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(771..2003)
                     /locus_tag="IXG20_001877"
     CDS             complement(771..2003)
                     /locus_tag="IXG20_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1610071.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2260..2829
                     /locus_tag="IXG20_001878"
     CDS             2260..2829
                     /locus_tag="IXG20_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1610072.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3007..3447)
                     /locus_tag="IXG20_001879"
     CDS             complement(3007..3447)
                     /locus_tag="IXG20_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ1610073.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3605..4369
                     /gene="map"
                     /locus_tag="IXG20_001880"
     CDS             3605..4369
                     /gene="map"
                     /locus_tag="IXG20_001880"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ1610074.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4401..5324
                     /locus_tag="IXG20_001881"
     CDS             4401..5324
                     /locus_tag="IXG20_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ1610075.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5400..6539
                     /locus_tag="IXG20_001882"
     CDS             5400..6539
                     /locus_tag="IXG20_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ1610076.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFTAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6532..7332
                     /locus_tag="IXG20_001883"
     CDS             6532..7332
                     /locus_tag="IXG20_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1610077.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7332..8159
                     /locus_tag="IXG20_001884"
     CDS             7332..8159
                     /locus_tag="IXG20_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1610078.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8137..8871
                     /locus_tag="IXG20_001885"
     CDS             8137..8871
                     /locus_tag="IXG20_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1610079.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8971..9837
                     /gene="rfbA"
                     /locus_tag="IXG20_001886"
     CDS             8971..9837
                     /gene="rfbA"
                     /locus_tag="IXG20_001886"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ1610080.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9851..10423
                     /gene="rfbC"
                     /locus_tag="IXG20_001887"
     CDS             9851..10423
                     /gene="rfbC"
                     /locus_tag="IXG20_001887"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ1610081.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10445..11473
                     /gene="rfbB"
                     /locus_tag="IXG20_001888"
     CDS             10445..11473
                     /gene="rfbB"
                     /locus_tag="IXG20_001888"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ1610082.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11571..12422
                     /gene="rfbD"
                     /locus_tag="IXG20_001889"
     CDS             11571..12422
                     /gene="rfbD"
                     /locus_tag="IXG20_001889"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ1610083.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12456..14489
                     /locus_tag="IXG20_001890"
     CDS             12456..14489
                     /locus_tag="IXG20_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610084.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14533..15813
                     /locus_tag="IXG20_001891"
     CDS             14533..15813
                     /locus_tag="IXG20_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1610085.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYGEQPDSAHSSRIQRYH"
BASE COUNT         4861 a         2732 c         3281 g         4951 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      301 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      361 atgcatgcgt atgtcttagt ccctcaataa acagtcgaag agaaaatagg caaaaattga
      421 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caacagattc
      481 gaattaaacg atttcgattg gcttatacca gtcctatccg acgaaagata tatcgttctt
      541 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
      601 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
      661 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
      721 tcctttattc tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa
      781 tcgccatttc ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc
      841 ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct
      901 aattcttcat cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt
      961 gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga
     1021 gaaataggtg aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct
     1081 gtatcaagca gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aatgactttt
     1141 ccatctttaa aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt
     1201 gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc
     1261 ggcatatttg ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga
     1321 tggtttttag gcaatccgat aatgatatct gtaccgggag cagtatagtg cagggcagaa
     1381 aattgttctc gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat
     1441 gctaagacag ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc
     1501 tcaacttgtt tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc
     1561 gcagccacga cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt
     1621 gctttgccag ccgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca
     1681 tcgggatcgg cagaaacaac actgatcctg cttgctcctt tttcaagcca ttcatccgct
     1741 tgatcgattt tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat
     1801 tctctttgga tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa
     1861 gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact
     1921 gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga
     1981 agttcattaa aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta
     2041 ttagcatgat accatttaat tatgattcaa acaatgaggc ctttaaataa aaaaaagagg
     2101 ttattttttt aaggttaagt ttcatgatat atgttactac ttgctatttc agcttgtgaa
     2161 gaaaatcaaa aactaatttt gcatatcaaa aaaaacatgt tacaatgaaa ttaatcaatg
     2221 gtggaagcgt tataaaaaaa ttaaggagat taaaaaaata tggcaagaaa aaagacgatt
     2281 actagagaac agattttggc agctgcttat gaagtagctg ccaccgatgg atttacacga
     2341 tttactgcga gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat ttatcttgag
     2401 tttaaaaata tggatgactt gagacaagct ctttttgagc agatctatga tcatttacaa
     2461 tatgaagtgt tcactgttga gcatacaggg aatacgatta tcgatcttgc ttttaattat
     2521 atccattttg caagaagaga gaagaagttg tatcgttcac tgtacttaga agaatatggt
     2581 ggaggaaaag agatgcagaa tttctcttat acttactttg tgaatacagt gaaacgcgat
     2641 ccagcttatg tgaatctttc agaagagcag atcgaatctt tgtataatgg gacatggatc
     2701 atagctactg ggatcgcagc tttgatgtct tcaagcatca tccatccaac tgatgaacag
     2761 attgaaaaga tgatacaaga cagtatagat gctatcttga aattcaaaga accaatagaa
     2821 atcgattgaa tatactcaaa agttccacaa atcagtagat aacaagagat gtgtcaaaat
     2881 aaggaaaagt tcgtttttct tacagcgtat gcatttttaa ctcccgaaga actcttccat
     2941 gaatgcaatg aataaaagag ccgtacagat tgaataaatc tggatggctc ttttattgca
     3001 agtatattaa atttttgcga ctagttcttt agcgaaagct tctaaatttt cgatatcttc
     3061 ttcttcagca ttcagatcca ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc
     3121 aaaagcagca tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag tatcaccaga
     3181 tccgcagaca ccgtaaattt ttcccgatag atccaattct tgcaagtctt cataaaagtc
     3241 tacgatctca tcaggcagat cgccatcatc gtatgtataa gtagctacta cgcaaatatc
     3301 tgcctcctcg aattcatctg catctacttg ggtacattcg ttgatttcga cttcgatatc
     3361 cagattttca aatgcttctg caacaatatc ggcgatttct tcagtgtttc ctgtcatgct
     3421 agcatatacg atttttgcta aagccatgat gtgcctcctc gtttataatt atttaacaat
     3481 tatgaattat accaaaacat cattgaaaag aaaataaaac ctagagacac ctggggaatg
     3541 tcatttggct aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa aaggagacga
     3601 aaatatgatt actttaaaat cagcacgtga aattgaaatg atggcagaat ctggagcttt
     3661 attagcagat gtacataaaa atctgcgtga ttttataaaa ccaggaatca caagttggga
     3721 tattgaggta tttgtcagaa actatatcga gagtcatggc ggaattgctg cgcaaattgg
     3781 ctttgaaggg tatgaatatg cgacttgtat cagtataaat gatgaaattt gtcatggatt
     3841 ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt
     3901 gaaaggcggc atttctgatt cttgttgggc ctatgtagtt ggagaatcta cacctgagat
     3961 cgaccgattg atggaagtaa cgaagaaagc tttgtacatc ggtattgagc aagcacaagt
     4021 agggaatcgg attggtgaca tcggtcatgc tatccaaact tatgtagaag gcgaaaatct
     4081 agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct acgattcatg aagaaccagc
     4141 agttcctcat tacggagaag ctggaaaagg gttgcggttg aaagaaggca tggttatcac
     4201 gatcgaacct atggttaata ccggaacttg gaaaatgaaa atggatccta acggctggac
     4261 tgcgtataca agagacggcg gactttcttg ccaatatgaa catacattgg ctattactaa
     4321 agatggtcca agaattttga catcacaagg agaagaaggc acttattgat ctatccagtc
     4381 aataggtacg ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga
     4441 tgcgctttat tgagacgact caaaagcgca tcactgattc agaaatgagt acgacctctg
     4501 tcgtcgttgc ttattattta ctcttgtctt tatttccatt gattattgct tttggtaata
     4561 ttttaccgtt tctccacatc gatcaagaga ctgtactgac ctatttacga gaagtgattc
     4621 ctgagaccgt ttaccagttt atcggtcctg caatcaaaga ccttttgaca caaagttcag
     4681 gaggcttgtt atccgtttct gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg
     4741 ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg
     4801 tccgtttctt ctcactaatt gtcattgtct tgttcatgat tgctattagt ggggtaacta
     4861 tcgttcttgg gttgggacag ctgattttgg atgctattca gccaatcgta caaatacccg
     4921 tgaactttat cgatcagttc caaactttga agtggccaat cacattggta gctttatttg
     4981 tcatcatgtt cttgatttat ttaattgtcc ctaatgctca gttgaagttg aagacgatca
     5041 ttccgggcac gatcttcgct actgtcggct ggatgctgtt atcacaagtg tttagtatct
     5101 atgctaggta ttttgcaacg aggatcagcg gttatcagat cataggaagt tttattgtac
     5161 tcatgctttg gcttaatttt gctgctacga tcatcatttt gggcggaatc atcaatgcag
     5221 ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat
     5281 ggttcaataa attgaaaact aaattttctc ataaaaaaaa gtagaaacaa gagttcttat
     5341 tgtaaattta tatcagtaat ttataataat tatgactctt aattaaagta ggtgcccata
     5401 tgcttgtttc ttttagtatg gttataagat tttttctgac aattttatta tcactgatat
     5461 taacacctgt attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa
     5521 gaagaataaa taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg
     5581 ttatatcgac tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct
     5641 tgattgcttc tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac
     5701 ctaaacaaaa aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata
     5761 tacgtattga ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt
     5821 ttcccgtaac gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt
     5881 tggatgggct ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat
     5941 attttttcct ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa
     6001 gtattatcgg tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag
     6061 gagctctatt cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga
     6121 cttttgtttc atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt
     6181 ttgctatcat taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc
     6241 ttcatcaccg actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg
     6301 cgatggcttt gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa
     6361 cgatcatatt gattattgcc atgttgttta ctgcaatttt attatttgaa ttgattggtc
     6421 tgatcaatga gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt
     6481 accgtacgca agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac
     6541 aaattaaacc agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg
     6601 acactagata attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt
     6661 gacaacaatt cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac
     6721 atcgatatct attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg
     6781 atagctagcg attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag
     6841 acagttacag cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct
     6901 aagattctta atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt
     6961 gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg
     7021 gctgtgtttg aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca
     7081 ggtagtttta tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt
     7141 ttcttcatgt actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag
     7201 ttattgtaca caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat
     7261 gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg
     7321 cgttttttct gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca
     7381 gtcaatctcc agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta
     7441 ttatttacga taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg
     7501 tttatcatca tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc
     7561 aagcagatga aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg
     7621 attattttga actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga
     7681 tgattttttc tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta
     7741 cagctcaagc agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag
     7801 gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt
     7861 tagatttttt ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga
     7921 tccttcctgt acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta
     7981 atcgataccg ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc
     8041 atttatccag tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca
     8101 aaaaccggca tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa
     8161 ttcgtgcgtt cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta
     8221 gatagtattt tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca
     8281 gtagatgata catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt
     8341 ttttcggggc ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa
     8401 ggagatctaa tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag
     8461 atcactgact attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt
     8521 gtggacagcg agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa
     8581 ggatggctta ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct
     8641 tctttaaaac ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt
     8701 ggtttgctgg gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga
     8761 catgatttaa acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg
     8821 cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg
     8881 tttaaatcct taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa
     8941 atttgaaatc aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc
     9001 ggaacacgtc tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac
     9061 aaaccaatga tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg
     9121 attatttcaa cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat
     9181 ttaggactgc agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt
     9241 atcattggag aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt
     9301 tactatggtg gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg
     9361 acagtatttg gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag
     9421 aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta
     9481 actggccttt atttttatga caatgaggta atttctatcg ctaaaggtat caaaccttca
     9541 gaacgtggag aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta
     9601 tccgtagaac ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg
     9661 cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt
     9721 ttggaagaaa ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca
     9781 cagccattga aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga
     9841 gtccttacag atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga
     9901 cgtctttggc gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga
     9961 acatggattg aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt
    10021 ccttcggggg cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt
    10081 cactggagca gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca
    10141 atgggacgga tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata
    10201 tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta
    10261 ctataatgca gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg
    10321 gccaattgat ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa
    10381 agaatttgaa gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt
    10441 tcttatgaaa aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca
    10501 ttacgtggta aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc
    10561 aggaaacaaa gagaacttgg ctggacttcc tagcgatcgc gtagaattgg tcgtaggaga
    10621 tgttgcagat gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta
    10681 cgcggcagaa tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa
    10741 tatcattggt acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca
    10801 cgtatctact gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg
    10861 ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc
    10921 ttctacaaaa gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg
    10981 tgcaaccatt tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat
    11041 tcctcgccaa gtaacgaata tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa
    11101 gaatgttcgt gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac
    11161 gaaaggcaga attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac
    11221 cgtgatggaa ttgatcttag aaatgatggg tcaaccaaaa gatgcgtacg accatgtaaa
    11281 cgaccgtgca ggacacgact tgcgttatgc aatcgattcg acaaaattac gggaagagtt
    11341 aggatggaag cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta
    11401 cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa
    11461 aaatggacaa taatacagag aaaatgtcac tgtattaatg gaaaaacaca tgtgaggcag
    11521 gcgctcgcct gtctcttttt taaaataaaa atcaaggagc ggataatcat atgtttttat
    11581 taactggagg aaatgggcaa ctaggtacag aattgcgcca tttattagat gaaaaaaatc
    11641 tgcaatatgt ttcaacagat gcaaaagact tggatatcac agatgccgag aaaacaatgg
    11701 cttacatcac agaattaaaa cctgatgtta ttttccattg tgcagcttac acagcagtag
    11761 ataaagcaga agaagaagca aaagaattag atgagaagat caatgttgat ggtacaagaa
    11821 atgtagcact tgcagcaaaa gctgtgaatg ctcaattcgt ctatatcagt acagactatg
    11881 ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa
    11941 atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact
    12001 attatatcat acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga
    12061 tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac
    12121 caacttggac acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat
    12181 ttggtattta tcatttgtcg aacgaaaata gctgttcttg gtatgaattc gcaaaagaaa
    12241 tattgaaaga tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag
    12301 caacacgtcc gcagtattca gtcatgagcc tgaaaaagac agaagagtta ggatttgtca
    12361 taccaacttg gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt
    12421 aatttccttt tatcaaatag aatttggagc atgatatgga aaaacaaaca agaagtgcac
    12481 gacgaaaaga aaagccaaaa gcgaaccagg cagattcgaa tttggtgctt gacctgtttc
    12541 taaaactccg ctatcttatt ggcgttttag tcatcgtggt catcgttact tttaatttaa
    12601 acggaagttc acttggtgtt tgggataaat atgtgtcgca aagagacgat gggaaaaaaa
    12661 ctgatgtgat ttttggacag aatcgggaag tgcggtcaga cgaatggctg gtgcagacac
    12721 ctttttacct ctcacaagct gaagaggact ttccgttggt gaacgaggat tattcattaa
    12781 acggacaaaa catgatcatt gcgtataatt ctcctgcaaa ggatattacg gttatcggaa
    12841 aaccattcaa ctggggattc tttttcctcg gaagagatcg aggactttcc ttttattggg
    12901 cgatgaaatt agtcgtcatg gtcttgcttg gattcgaagt actgatgata ttaacgaaga
    12961 gaaaaaaagc tctatcgcta atcggtgcat ttgcgctgac attttctcca gcagtccagt
    13021 ggtggttcat gcaacatgtg ggcgatttga ttttctttac tttgggactg atgactgctt
    13081 tttatcatta tttctacaat catgataaaa aatggctacg aacgttgatg atgcttttgg
    13141 tggttatttt cggtattggg tttattttag tgatttaccc agcccatcaa gtgatgctgg
    13201 cgtacttgct gattttttat tttgttggcc tgcttattta ctatagcaag aaagtttcat
    13261 gggatctgtt ggatgtagtg ttgattggag gggcagtttt atttattggc ggagtcatgg
    13321 ttcatttctg gctaacgtca aaagacgcat tgttagcttc tttgaatacc ttatatccag
    13381 gaaaacgggt aagtactgga ggaaagtgga cgattggcga atttttttct ttccttacaa
    13441 attggaaaat tccttttgaa gatatcaact attcgaataa ctctgaagtg gccctgttct
    13501 atcacttttt tccaactgta ttcctagcaa gtccatttgt gttatttggc aaaaaagata
    13561 gcgaacaaaa gttactggga cgtattttga tgattttttg tctgttttct atcttttgga
    13621 ttaccgttgg gttgccaaaa tggctagcag aaatcactct gctgtcttat gttcctccat
    13681 taagagccat ccttactttt agttttgcgg cttgtttatt atcgatctgg tttatcgctt
    13741 atatttggag agaagatgtt attcctattt ggtataaatt cattttgttg gcagtaaatg
    13801 catggtctta tttctatgcg attacgcatt cgaaaataga taattatttt tcggattttg
    13861 aaaccatcgc agttgctgtg ttgggaacag tgattttagc atttgcatta tttggctgga
    13921 gacgcttgtt ttacctcgct tttgccgcct tagttatcgt atctggcttt ttcgtcaatc
    13981 cagtcgtaga aggtacagga gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa
    14041 tcgatcaaca agatcctggt aaagtttggt tgtctgaaga tgaattatat aacttcaccc
    14101 caacattggg agtgaaatcc ttcaatacag tacgctttta tccagatata gctgcatgga
    14161 aggaaatcga tccgaaagaa gaatacgaga agtattataa tcggtatgct catacaagag
    14221 cttttatcgc taaggaagat acaaagttca ttttagatcg tccagatatg ttttcggtca
    14281 cgttgaattt tgaagacgca gaaaaattag ggatcgacta tgtgatcagt aaacgaccat
    14341 taactgatta caaccaatta tatgaagcgc agttcacgca gctatatgga ccggataaag
    14401 acggttatat gatttttaaa gtagaatatc cggattatcc agatgtacca caaacacaaa
    14461 ctcaggaaaa tcagctagtc gtacagtaat aggctagctg agaataatcc tgaaatatta
    14521 gtagaaagga gagtggagat aattcaagca atcgtacaaa aatttgtcaa caaaatgagt
    14581 gtcggtttct tgcttttgtt gctgacgcta gtcttttttg tttggacagc agggttaggt
    14641 tttccagcaa ctttgtttat atataacaat agttcattac ttatcgcttt tagtgtggct
    14701 attttgctca ttttaaatgt ttataaagca aaaggcgtcg attttcttta tctaggtgtt
    14761 gcgcttgccc ttttcatttt cttttcttac tttcgggaat taagaaatga gtctttattt
    14821 ggagatttac tgtttccttt gattatttta ttttgtttag taattaaatg gatcgagtgg
    14881 acaaaatggg atcgcggtat ttatctgatt gtctttacgg tcttcctcgg aatcacgtta
    14941 tatcgtgtat ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat
    15001 tcgctttctg atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct
    15061 ttattgatca gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca
    15121 gtagcagttg ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc
    15181 gcgttgattg tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt
    15241 gcgccattta ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg
    15301 gctgtctctg aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat
    15361 cagacgctag gagaaaaatc tgaacaagtt ttactaggaa tgaaaacatt tatcttccaa
    15421 cgaggaaacc aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga
    15481 ttgataggat ttgggattgc tactttgctg cttatccttt ttattggtcg cttaacgttg
    15541 aaagcagatt tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg
    15601 cagtcattta tggaggatac attgacttca tacccatggg ttccgattat ttatatcctg
    15661 ttggctatgg ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg
    15721 gaagaggcat atgacgatat gacttatgat gatacatctt atggggaaca accagatagc
    15781 gctcattctt ccaggatcca acgctatcat tgataggcac aaaat
//
LOCUS       DACEUF010000061        15823 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.064, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000061 DACEUF010000000
VERSION     DACEUF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.064"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(56..358)
                     /locus_tag="IXG20_001892"
     CDS             complement(56..358)
                     /locus_tag="IXG20_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610086.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(578..944)
                     /gene="ssrA"
                     /locus_tag="IXG20_001893"
     tmRNA           complement(578..944)
                     /gene="ssrA"
                     /locus_tag="IXG20_001893"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1115..1888)
                     /locus_tag="IXG20_001894"
     CDS             complement(1115..1888)
                     /locus_tag="IXG20_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1610087.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2032..2382
                     /locus_tag="IXG20_001895"
     CDS             2032..2382
                     /locus_tag="IXG20_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1610088.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2363..3079
                     /locus_tag="IXG20_001896"
     CDS             2363..3079
                     /locus_tag="IXG20_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1610089.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3079..4527
                     /locus_tag="IXG20_001897"
     CDS             3079..4527
                     /locus_tag="IXG20_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1610090.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4597..5106)
                     /locus_tag="IXG20_001898"
     CDS             complement(4597..5106)
                     /locus_tag="IXG20_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610091.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5096..5821)
                     /locus_tag="IXG20_001899"
     CDS             complement(5096..5821)
                     /locus_tag="IXG20_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610092.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6044..8164)
                     /locus_tag="IXG20_001900"
     CDS             complement(6044..8164)
                     /locus_tag="IXG20_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1610093.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8394..9572
                     /locus_tag="IXG20_001901"
     CDS             8394..9572
                     /locus_tag="IXG20_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ1610094.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9588..10346
                     /gene="aroD"
                     /locus_tag="IXG20_001902"
     CDS             9588..10346
                     /gene="aroD"
                     /locus_tag="IXG20_001902"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ1610095.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10369..10854
                     /gene="ybaK"
                     /locus_tag="IXG20_001903"
     CDS             10369..10854
                     /gene="ybaK"
                     /locus_tag="IXG20_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ1610096.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10925..12178)
                     /locus_tag="IXG20_001904"
     CDS             complement(10925..12178)
                     /locus_tag="IXG20_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1610097.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12295..13644
                     /gene="gdhA"
                     /locus_tag="IXG20_001905"
     CDS             12295..13644
                     /gene="gdhA"
                     /locus_tag="IXG20_001905"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ1610098.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13756..15102
                     /locus_tag="IXG20_001906"
     CDS             13756..15102
                     /locus_tag="IXG20_001906"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ1610099.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15410..15796)
                     /locus_tag="IXG20_001907"
     CDS             complement(15410..15796)
                     /locus_tag="IXG20_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1610100.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5173 a         2829 c         3180 g         4641 t
ORIGIN      
        1 tttataagta tggtgataaa atccgttccg ggctaccttt tttaggtagc ctactttaat
       61 ctttatacct ttctggatca acgaaagtat acttcaaata gtcataacgc cgatgatcgc
      121 tccgtgcgtc tggcacatca gtcacgatat caaacaaaaa atacacatct ttcttcattc
      181 tggtttttgt tgccggaatt ttaaagtagt tcttattaga gtaatagaga ttgattaata
      241 agctatcttc gattgctaaa aagaaaactt ctgaatccca caccttataa aaatctttga
      301 caaatctatt cgaaggatca aatttaaacc ataattgtgt ttttccttcc atcaacataa
      361 tgttcacctc aaaaagagta tacgaactaa tgttcttttt gtaaagtagg aatatcattg
      421 ataatatgga tggaacatat atattaaaaa gtgttctttt tgaatgctat tttgaatgcc
      481 attttcattc atttaacgaa aatctgcgaa aataaaaaat gctcaaaccc ttataaaata
      541 aggatttaag catctagcga aatctgctga aatctcacaa tggagacggc gggagtcgaa
      601 cccgcgtcca aacacatcgc cacttaaaaa tctacgttca tagtcaactc atttaaggat
      661 tcactttaca gcttgccgag tgacaggcat tctgtatcgc tagtctgatg ttctcttttt
      721 caacttacag acggaaagtt gaaacgtatc ccactaaagt taggaccctt actcgagcac
      781 atgggcgatg ccgagaggat ctacgctaac tgtttttagg cagctaaagc gtaagagttg
      841 ttttcgtttt tagcagttat atttaactgt aacgttttta cgtagacgta acctacgaaa
      901 cgcaattcaa gctcgactgt gcctgtcgaa tccgtaacgt ccccgttagc taacaagggt
      961 tacgtgtatt tttcatacaa aattagtata gcatatattg ggataaattc aataaaaaag
     1021 cgaatcatat cccattattt atataataac tgcccctttc ttaagaaaaa ataagcgatt
     1081 ttcttaagaa aagaggtagc ttcaaaaaaa gatcttaaat cagatcaaaa tacttcaatg
     1141 cgtggacaat tccgccttct gtatttttct ttgtgataaa ggtagaaagt tcttttaatc
     1201 cgtcataggc gttgcccata gcgattttgt tgtcgcaagc ttccaacaag gcaaaatcat
     1261 tcggaccatc gccaaatcca aaagtaggaa ctttttccag ctgaagttct ttttgcagac
     1321 gtttgacacc tgcccctttt gagattcctt tcttcactgt atcgatcgag taaggaccat
     1381 tccgataaaa agttagctca ggaaaatgtt cgtgatagta ttcgtcacct gattgagata
     1441 agatcagcaa catatttact gtgttttctt taaagcctaa aggatcaatt tccggaacat
     1501 ctgaatgaat gaagtcgtaa gcattgatca ttgtttggct atgaccggta caccagattt
     1561 tctgatcgtt atagtaagaa agttcatgac cgttcaactg tacatgatca tacatttttt
     1621 gacattcttc tgaagaaaat gtttgagaat agatttcttc gccgtctact cgaatgaaag
     1681 aaccattcat ggtgaccgca gaagtaatgc ccgcatcttc catgatagag tggatttctg
     1741 cttctgtacg tcctgtagcg atgacgggga gaacattgtt ttcttttaat gcgttcatag
     1801 cttttgccac ttcgggtgtg atttttgatt tcgcatctag taatgttcca tcaagatcaa
     1861 agaaagtgat tgcacgataa tttgacacaa ataaaactcc ttttttattg gaataagttt
     1921 atcttaacaa agaatgaaca aaagacaaac ctttcgtgaa gaattcctga acatattggt
     1981 tcagtcaaca atttagagta tactataaga agagaattta ggagggaaag aatgattttt
     2041 ttcagtattg gtatcttatt cttaataatt ggcagtcttt ttcggattct gccctcaaaa
     2101 ggaaatctgc ctttttatgg gtatcattcg cctcttgccg caaaaacaga cgctcattgg
     2161 cggctggctc aaaagacaag cggaaactgg ttttttctta tgggcttact gatggcattg
     2221 atcggctatt atttaaagac atctgggcat acgaattatt ttctgattga aatgcttctg
     2281 ctagtatttc caattatgcc tatttttatt atgacagaaa agaaattaca gaaatatgac
     2341 ctggaaactg gagggaacga taatgaatat tttaatgatt gaagataatc aatctgtttc
     2401 tgaaatgatg cagatgtttt tcttaaatga aggttgggaa gcaactttca agtatgatgg
     2461 aaaagaaggg ctcgatgcct ttttagaaaa cccacaacat tgggatatga ttacactaga
     2521 cctgaatttg cccacactgg acggtatgct agtaggaaga gagatccgca aagtttcaag
     2581 tactgtgccg attatcatgc tgacagctag agattctgaa agcgatcagg tgatcggctt
     2641 agagatgggg gcagatgatt atgtcacaaa acctttcagt cctctgacgc ttattgctcg
     2701 aatgaaagct ttgcatcgcc gtagtgagtt ggcagaagca agagaagaag cagtagtttc
     2761 agatgatcat tttgatatcg agactagtca tttcaagatg aatacgaaaa cacgagaagc
     2821 gtatctggac aaccggccaa ttgaaggact gacgcctaaa gagttcgatc tattgtttac
     2881 tttagcaaaa aaaccacgtc aagtattctc tcgggagcag ttgcttgaat tagtctggga
     2941 ttatcagtac tttggtgatg aacggacagt ggacgcccat atcaagaaat tacgacaaaa
     3001 aatcgaaaag gtcggaccac aagtcatcca aactgtttgg ggagtcggct acaaattcga
     3061 tgattctggt gttgcctaat gcgttatttg tatcagcaac tcttagcttt ctgcggaatg
     3121 gtcgcgttga tcttattgat cgttggagtt tcttttacac aactgacaaa acaaacgatt
     3181 gaagaaaaca attaccagca actgtttgga tatgctgaat cagttgaaaa gacaacgcaa
     3241 acctatgccg atacgctccc gcagtttagt caagatcagg cgtttcaaaa tgcattgctg
     3301 ctgacagagc aagtactgac agaacaaaat gtgaacttta tatttatcga taaaaacgaa
     3361 cgtgtcattt atccgactaa tacagcttta ctgaatttta ccttaacagc tgatcaatgg
     3421 aaaaatctga ggaatggacg tcaagaaaaa ttcacatcga ataaaaatat tttaggacaa
     3481 gcacaggcga cctcttacgc acttgtgcca tttaatttga atcatgaatt ctatggagca
     3541 cttgtagtta gtcaacctgc gcgaaatatc gataacagcg ttcgtccaat tacactgaac
     3601 ctgttcaaag gatttatttt ttccagtatc gttgcagtta ttgccagcta tgcttttgca
     3661 gcattccaag ttaagcgtat caatcggtta aggaatgcga cgaaagaagt gacgaatggt
     3721 aattttgatg tacagctccc cgtacatgac aaagatgaat ttgatgagtt agcagatgat
     3781 ttcaataaaa tgacgaattc attgaaagaa tctcaagcag aaattgaaga acaagaaaat
     3841 cgtcgaagac aatttatggc agatgcttct cacgagatga gaacacctct tacgacaatc
     3901 aacggacttc tggaagggtt agaatacaat gcaatccctg aaaatcagcg agaaaacgcg
     3961 attaaattga tgaaaaatga aacagagcgt ctgatccgtt tggtgaatga aaacttggat
     4021 tatgaaaaaa tccgtacgaa tcaaatcagt atggtcatta aaaaattcaa tggaacagaa
     4081 acattgaaaa atattgttgc acagttagaa gctaaagcag aagcagcagg agatacattg
     4141 acattgaaag ccgttgatga tattgatgtt tacgctgatt atgaccgatt tgtccaaatc
     4201 atggtcaata ttattcaaaa cgcgatacag tttactgaaa acggccaaat cacgattact
     4261 ttagaaaaag gctatcttga aacgatcatc acgattgaag atacgggaat tggaatgtct
     4321 gaacaacaaa tgaaaagtat ctgggatcgt tattataaag tagatccttc aagaaaaaat
     4381 acaaaatatg gtgaatcagg attaggttta ccaatcgtgc agcaacttgt acgcttgcat
     4441 aaaggaaaat tagaagtaga aagcgaacta ggaaaaggaa caaaattcgc tgttcgtttg
     4501 cctgacgtag aaatcaaaaa agagtaaaaa acaagcggta taaaagatca tgaatcgatc
     4561 ttctataccg cttgttttta ctataaaatg attgatttag cccgcgggct ctaaacgatc
     4621 aatataagga ctttgagcaa tagtagcatc atgagtaaag agtaagatac cttgattttc
     4681 ttcatgagct aaataagaaa gcaagtggag tagctcttgg atctccaata cactaagttc
     4741 atcaaatgta tcttcaataa caatattgtt tcgcttggaa agcaaggcat gaagtaattg
     4801 cagctttact ttttcaaaag acgtcaactc agctgccgca caattaagca aggaaggaga
     4861 gatatcaagt ttttcttgat aggccaaacg atcttgtttg atttcttttt ccggtacatc
     4921 caacaggaca tggtctttaa gattcagata aggaaacatc ggccaatttt tttggatgat
     4981 cgcaaaatta tccagttttg caacaagaac ctcttttttc aagtgctcca atattttatg
     5041 ctgtttttcg agaggacaca aaataacggt caagtgttca gcatgaatca aatattcagg
     5101 tttcattacg tccaggcctc ctactttgca aatgatggat aaaaaaagtg aaagggaata
     5161 gactaagaat gagacagcat ccactgagcc caataagagt ttgggcaaat cccttaagta
     5221 tatctgtgaa aaaaagatca ttttggaaat cgatggtaaa aaaagtctgt tgatccatcg
     5281 gaattgtaag tccgcttacc gaagtcaaga tatcacggat atctggcagt tcagtagtct
     5341 ttaagacaag aaaattgatc ctttgaagaa gcgcttcata aactgactgg aagcttacga
     5401 tccacaataa aaccaagata caacaaagaa agagaggaaa gaacagctgc cagaaaataa
     5461 aaaggaataa tttggtttta gaaagaccag tcaggtgcca attgatcaat tcctgcttgt
     5521 tttgcctgac tgaatagtaa aagaaaaaga taaaaaggaa ggcaaataga aaaaataaac
     5581 tgaatatgcc ttttatataa aatcggctag ctaattctaa agccggaaga agctctgtag
     5641 atgcagaagc gccaataaat gaaagccgat cttggacttg attggctaaa gccgattgca
     5701 aatggtaaag agtcaggcat gctatcaata aaaaagcaga aaagcttgaa aataaacaat
     5761 agagtattgt ctgctttttg taataaatta atcgagcagt agcatagcgg attattttca
     5821 tacttttcac ctctataaaa agtatagaaa atgaatatga ataaagtatg aaattccttt
     5881 agtgaaatca cgaattaaaa ccagaaaata ttttgtaaaa agcaagaggt tgtgaagaag
     5941 ctgccctatt ctattaccgt agatgcaagc ttttgaacac tgtttattcg aaaagaggag
     6001 cagcgacaat aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg
     6061 tggtcgttcc gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc
     6121 cgttgtgttc actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta
     6181 atttctctaa gccattcttg tagtcataat cactggtatc aattgggtct aatccactat
     6241 tcgtatagaa ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat
     6301 caactttttc tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag
     6361 cttcaccagt ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa
     6421 aatcaccatc acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta
     6481 actgaatata attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa
     6541 cttgtccgcc gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat
     6601 aagggacacg ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa
     6661 ctaattcagc tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga
     6721 cgatgactga attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg
     6781 cggtatctaa ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg
     6841 ctaacgggaa tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag
     6901 caatgaattt tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga
     6961 aaaatggttt atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag
     7021 aagaatcaaa gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg
     7081 taccagcgtt cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag
     7141 gtgcagcttg gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc
     7201 caaacaatga attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat
     7261 ggaaaaagtt gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg
     7321 taacttcatg ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga
     7381 agctttctaa atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat
     7441 ttggtgctgc ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg
     7501 cttctgcccg cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat
     7561 tcaagccaag atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac
     7621 gatcagcttc cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg
     7681 caaaagccat tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca
     7741 gaacagtcaa gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa
     7801 tggcactttc tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa
     7861 tgaagtccgt gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa
     7921 aataaatgac catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc
     7981 caagtaataa caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac
     8041 tttcgattcc gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa
     8101 cgactagtaa agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt
     8161 tcaagataat cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca
     8221 cagctcactt attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga
     8281 agtttatgct gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca
     8341 agtcctatgt tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag
     8401 taaaagtaaa aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag
     8461 aagaccttgc tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa
     8521 accaatttat cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga
     8581 gccagcagaa catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag
     8641 aaaaaagaac tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag
     8701 aaggagacgg gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt
     8761 ggtataacga aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct
     8821 acccggaaat cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga
     8881 gccagttcat ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa
     8941 aatttgccgt ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag
     9001 tacggggatc attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt
     9061 ataccggcgc cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg
     9121 atctagcaaa acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg
     9181 tcgatcagca aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa
     9241 aacaattttt atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa
     9301 aagtctttcg cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag
     9361 cagaagacgg gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc
     9421 aaacaatgat tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat
     9481 atcgtctgcc aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag
     9541 tgtttgttta tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa
     9601 agggattgaa tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc
     9661 gagaggaagt attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat
     9721 ggcgagtcga tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc
     9781 ttatccatga gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag
     9841 gcggagaaca agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca
     9901 caggattagt agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac
     9961 tgatagagga aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag
    10021 aaacaccagc agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg
    10081 atatcggaaa actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt
    10141 tgactagacg ggcaaatgct tttgtctcca tgcctattat tacgatgtcc atgggaaatc
    10201 ttggtaaaat ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct
    10261 taagcagcga ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta
    10321 ttcaattatt aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa
    10381 aaaatagtaa aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag
    10441 cacgaatatg agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca
    10501 gaaagccaat ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt
    10561 gttgctgtga taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg
    10621 aataaaaaag tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt
    10681 ggtggctgct cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca
    10741 gaaagctatg aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca
    10801 cctcaagata tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata
    10861 cgtataaaag aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta
    10921 tacattatgc tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg
    10981 tgatattaaa gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac
    11041 catcaatgat tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc
    11101 cagttacaaa tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc
    11161 atagttgaac aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca
    11221 tttcaagccg ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat
    11281 tgaccaagtt ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt
    11341 tggtttgtgt agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt
    11401 ctttattgac aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga
    11461 aaagtaaatc catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag
    11521 tgatgtcttg taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata
    11581 tcaaggggga ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct
    11641 tgtacccatc tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa
    11701 tggcaggagc atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt
    11761 ggatcaattt ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat
    11821 tgagcaatgc ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat
    11881 cctcttcttt taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc
    11941 attgcgcaaa cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat
    12001 caacaggacg attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc
    12061 ctgctttttt gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg
    12121 ctgccatcat cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc
    12181 cctcacctca aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag
    12241 ccaaaagaga aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca
    12301 aacgcaacag aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa
    12361 gaagaatttt tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact
    12421 catcccgaat atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt
    12481 caatttcgag tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt
    12541 atccaataca actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc
    12601 aatctaagta tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt
    12661 ctgccaatcg gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat
    12721 gagatcatgc gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg
    12781 attgatgttc ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc
    12841 gaatataaac gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt
    12901 tggggaagta agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat
    12961 ctattgaacg aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg
    13021 aatgtagcca tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt
    13081 tcagattcta gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa
    13141 gtcaaagaaa tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt
    13201 tatcatgctg gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt
    13261 gctactcaaa atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa
    13321 atcgtagcag aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa
    13381 gcaggcgtgt ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct
    13441 ttagaaatga gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag
    13501 ctggatcaaa tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc
    13561 gacgaaaaaa atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca
    13621 atggcagcag aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt
    13681 gtttatttgc ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta
    13741 taaggaggag aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc
    13801 aacgaacaag agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg
    13861 gaaggtactg gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac
    13921 aaagaagaat ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta
    13981 ttagttgtta tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg
    14041 aatcacacat tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt
    14101 gccggaaatt caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc
    14161 gacttttcag taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc
    14221 cgtgtattca aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc
    14281 tacgctacaa cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg
    14341 gaaactttcg tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt
    14401 ttgttaccaa tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat
    14461 gcaagcaaag catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca
    14521 atgcgcaaca ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca
    14581 ggaatgcaat acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac
    14641 caaaaaggca tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa
    14701 ttcatccaag aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt
    14761 aaatcaatca caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga
    14821 aaagaagtgg acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac
    14881 ggtgacgtgc ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat
    14941 acaatgtact tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca
    15001 ttcgaccaac caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca
    15061 ggctttgaag atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta
    15121 gatttaaata atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta
    15181 ggtactcaaa catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca
    15241 cagtcccttg tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact
    15301 ttaagaacag tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt
    15361 aagcaaaaaa gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc
    15421 ctaatttttc ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga
    15481 tccgcttttc gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg
    15541 cagagtcacc agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat
    15601 aagcatctac gttgtattta aaacctcttc caattacttc cccattatct gctttgatat
    15661 cagtttctgc aggcattgga gctttgacta ccgcataata atctttacct acataagtag
    15721 actgatagta gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc
    15781 ctaaaatttt tttcatgaaa gatctccttt tttattttta tta
//
LOCUS       DACEUF010000062        15010 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.065, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000062 DACEUF010000000
VERSION     DACEUF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.065"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(273..1154)
                     /gene="galU"
                     /locus_tag="IXG20_001908"
     CDS             complement(273..1154)
                     /gene="galU"
                     /locus_tag="IXG20_001908"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1610101.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1197..2216)
                     /locus_tag="IXG20_001909"
     CDS             complement(1197..2216)
                     /locus_tag="IXG20_001909"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1610102.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(2849..3853)
                     /locus_tag="IXG20_001910"
     CDS             complement(2849..3853)
                     /locus_tag="IXG20_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1610103.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(3866..4915)
                     /locus_tag="IXG20_001911"
     CDS             complement(3866..4915)
                     /locus_tag="IXG20_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1610104.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(4932..5753)
                     /locus_tag="IXG20_001912"
     CDS             complement(4932..5753)
                     /locus_tag="IXG20_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1610105.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(5740..6519)
                     /locus_tag="IXG20_001913"
     CDS             complement(5740..6519)
                     /locus_tag="IXG20_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1610106.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(6536..7006)
                     /locus_tag="IXG20_001914"
     CDS             complement(6536..7006)
                     /locus_tag="IXG20_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1610107.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7018..7437)
                     /locus_tag="IXG20_001915"
     CDS             complement(7018..7437)
                     /locus_tag="IXG20_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ1610108.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(7518..10286)
                     /locus_tag="IXG20_001916"
     CDS             complement(7518..10286)
                     /locus_tag="IXG20_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1610109.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            10486..10710
                     /locus_tag="IXG20_001917"
     CDS             10486..10710
                     /locus_tag="IXG20_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610110.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            11489..13204
                     /locus_tag="IXG20_001918"
     CDS             11489..13204
                     /locus_tag="IXG20_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1610111.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            13207..14970
                     /locus_tag="IXG20_001919"
     CDS             13207..14970
                     /locus_tag="IXG20_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1610112.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         4362 a         3096 c         2585 g         4967 t
ORIGIN      
        1 aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat ttctcttatg
       61 taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa tattttctga
      121 atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg atctagagtc
      181 aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa taacaaatag
      241 cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag ctttttactc
      301 aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag tccatattca
      361 atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc tttaaactct
      421 ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag ctgtatctcg
      481 tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt cagtagataa
      541 cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc tacaaaccct
      601 tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc gtatttagat
      661 gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc ttcataatcc
      721 atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc cagcatgacg
      781 acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc cagtcctctt
      841 ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac taaagcgagt
      901 aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc atcaaagtga
      961 tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat tcccgaatcc
     1021 aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag catttctttt
     1081 gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg aatgactgct
     1141 tttttgacct tcacttgctg ttcctcctaa acgacgatta actattgatc atctatttaa
     1201 cgaagagaaa attcattctc catttttccg tcacgtaaca tgatttcttt tgctgctttt
     1261 ttgatatctt ctccattata caaaacatta tagattgttt gagtaattgg catctcaaca
     1321 cctagctgtt gagctagctc tactgctgct tttgtcgttg ccacaccctc aacgaccatt
     1381 cccatgtttt ctagaacttc ttccagctta tgtccctgtc ctaacaagtt accggcacgc
     1441 caattgcgag aatgaacact tgtacaagta acgatcaagt cccccacacc actcaaaccg
     1501 ataaatgtca gaggattggc acccatcgcg acaccaagac ggctgatttc tgccaatcct
     1561 cgagtcatga ttgctgcttt tgcatcatct ccgaatccta atccatggat agcgccggca
     1621 ccaattgcaa tgatattttt cagtgctgca ccagtttcta cgccaatcac atcattattt
     1681 gtataaatcc tgaaataatc attcatgaac agtttttgta cataggctgc tgctttttga
     1741 tcaatacttg cagctgtaat tgttgtgata tcatgtactg ccacttcctc agcatggcta
     1801 ggaccagata atacaacaat tgcctgtctt ttttcttctg gaatttcttc agccaaaact
     1861 tctgagattc gtttatgcgt atcttgttct agtcctttac ttgcatgtat aatgattggc
     1921 ttagtattca ttttaccaat aagttcctgt gcgactgaac gaatcgcttt tgttggaaca
     1981 acaaataaaa tagcatctgc gttatccact gcttctgcca attctttata tccgataatc
     2041 gattctggga tcgtcaaatc aggtaaaaaa tgctgatttg tatgatactt attgatttca
     2101 tcaatctgtt caggtacatt tccccaaata cggacatcat gcccgttttc tgccaatgtt
     2161 tgtgctaaag cagtgcccca agatccagga ccaagaactg cgattttttg tttcatggat
     2221 attcctccaa ataaataatt gataatttcc attatagtat acatttctta aagttgggac
     2281 taatttccat tgtttttaag tgtttgttta cttcttttat cgtttttact tgaaataatc
     2341 aatgatatgt tacaatagtc tacattttat ttttacactc gttatgcttt gcttgttcct
     2401 aagaggaagc agttttgaca atgttactac cagcattact attaggaggt taggaaactt
     2461 ctcctagtga gtattgattt aaaatacata agagacagat tgaacatttc taaatctgtc
     2521 aggtcctgca acggacagat tgtctctgtt gacggttgac ataggaactt ttgttttagt
     2581 caacattctg gatacaaaac gctatgtaaa ctggcgcaac tcgttgtact tggactttta
     2641 ctacggatcc atgcaacaac taaatagtac tattgcataa caaaaaatgg ttggcaccca
     2701 catgtcccat taaacagatt ataagttgaa ctgtagataa tgcaaaaaat gtgttttata
     2761 acgtaattga tgccaatatt ttaaaaaaat aaagggagca gttatcgctg ctctctttat
     2821 tttttatatt ctttttatcc agtctatcct aataatcaaa ctgacgatag tagcggcgta
     2881 tctctaatgg atggcagttc aactgctcca catgagcatc gatgcgttgt gtcactacat
     2941 gcatcaataa gaaagacaaa acacgtccgc gatatttttc actgatacct tctactggat
     3001 agtcttttgt gtctatcaat gtataattgc tgcaaatttt tggcaaaagt ttagccacac
     3061 gttcagataa cactctctgt tcatcttcgc ctaaaaataa tgtgactggg gttgtttctt
     3121 ctacgatctc taacgtcccg tgaagaaact cggcagcatg gatagatttc gatggaagcc
     3181 agctttgttc ttcccagtaa cacatggcat atgaataaac agctccccat tgattccctg
     3241 ctccaataaa ataatgcata ctatcacgtc gatgtttttc tgcaaatgat aagccaaatg
     3301 catctgcttt tttctctgct tccactaacc ctgttggtaa ataacgttcg agctgttcat
     3361 agtattctga ataagcttcg aattctccat tcaaatacat caatcgatct gctgccataa
     3421 aaaatttgat ttgttctgtt ccaggagctg gataagtgac cacaaaatcg catagctgag
     3481 acaacttact gttcgcttta tcgataaatc caatcagtgt cgctcctact ttcttgattt
     3541 gttctactgc tttcactact tcttgagttg taccggtaac agaggacaaa ataacgatag
     3601 aatcttttgt taatcttttg tttccagtag tgtaatattc tgcagcatgc tgtacaaaga
     3661 caggcaagtt gcttttccca ttcatataag tgacagcttg catcgaggaa gcatacgttc
     3721 caccgattcc tagataatag acagcgtcaa atttttccgc ataaagttta tcgatgattt
     3781 cttctacttg cggacgtaaa gctagtgcgc cttttattgc tttgatttgt tcttcttcat
     3841 tgaatttcag catgatttcc acctcttata atttactttt caagactgta tcaaagtctt
     3901 caaaaatctt tttgttcaag tcaatacctt tagctactgc ggttttataa gcgaatagtt
     3961 gaaacggaac gacaagcgct aatacagaca gatattcatc acattctatt gctaatccaa
     4021 gtgttttctc gtttcttgct ttttttgtgg taatggtgag agtctctcca acgtaccgac
     4081 tcatatatct ttgcaacttt tgcgaacgtt ccttatttac actttcccct tcaataaaga
     4141 atagcaaatg cgaagcatct gcctccaggt atgggccgtg catataagct tctaactcaa
     4201 atccttgtga cggaacgcgg attgtttcag tcaatttcgt ctctgcttct ttagctgttc
     4261 cccagtttgg accatatcca atggtgacga aacgctgtgc taaacgaaat aaactttgat
     4321 atccatcaaa aaattcttcc gttttttgaa taatcgctgg taaatggttg attattttgc
     4381 gcaactgttg catgtagtct tgttctattt ttttgctgat tttattttta cttattccaa
     4441 ttcctatccc taagagtagt aactgtaaaa cagtcgcaga atagcctttt gtaacgaagc
     4501 ctactgattc taccccggca cctaaatcaa taatttgtgc agcttctaaa gcaagtggac
     4561 tttgcacgtc atttgtgata gcaacaacag gtaacccttg tttttgtatc atttttactg
     4621 cttcaacagt agaagcactt ttcccgcttt gtgacacagc aatcacagta tcgattgaag
     4681 gagaaagttt tccataattg aaaaaataaa aaggttcttg aatatcgaca gtaagatcac
     4741 cataagattc caatgctggc ttcgctgcta aacaagcgtt ataagatgaa cccgttgcta
     4801 agatcagtaa attcactgtt tttttcatat gttctcttgt cgaaaaatca ttctttttca
     4861 agatcgtact tagtacttcc ttttcttcat agatataatc ttgcatcgta aacattttct
     4921 ctgctccttt tttaaaaaat ccctatccac gaaccaaaaa ttccaaagaa agctagtccg
     4981 atcaagatcg ttaatggttt cacttctttc ttcaatagat aaaacactat gccaaatgct
     5041 ccaagacata agatatttgg aacgatatca tcaaaaatac tttgaacagt cacagcattt
     5101 tctcctgaac cgatttttaa aggaatattg atggtgatca tcgtggcagt cattgctcca
     5161 acgaccatca gtccaataat agacgcgcct aatgtcaaac tttccatcat accgtttgcc
     5221 tggatctttt tcaaaaaacc cgttcctaag cgatatcccc aacctgttgc tagatagcga
     5281 aacagaatgt gcggaatatt gaaaatcaaa atgaacaaga tagggcctaa aatatttcct
     5341 tgcatggcta aagaggtacc cacacctgtg gcaatcaatc gcaaagttcc ccaaaagaaa
     5401 gaatctccta accctgcaag aggtcccatc aaagatgttt tgatactatc gatcgcagtc
     5461 acatcaaatt caggatcatc cttattttct tcctccattg cagccgtgat tcctaaaatc
     5521 agtgtaacga tatgtggggt cgtgttaaaa aactctagat gccgtttcaa agctgctgcc
     5581 atttcttctt tcgactgata taactttttc aaaatcggaa tcattgcata aacataagcc
     5641 atattcatct gacgttcata attccaagaa aactccattt gaaaagaacg ccaaaatacg
     5701 tgatttagct cttttttcgt tattttgctg tttttgatct cagaattctt catcttcttc
     5761 tacctcccct gcagttgttt gaagtggtag ttgacggaca ttcataatat tgactaccac
     5821 aactgctaaa atcgcaccaa aaatagctac tccggtcaca ggaatctcta agtaagaagc
     5881 tatagcaaaa cctaagaaaa aatatggtgc aacttgtttg ttgatcagca aacgagcaag
     5941 catcgcaaaa ccaagagctg gaatcaaccc tgtagctact gacaacccct tttgaacgaa
     6001 gtcaggaatc atatttaata gatttccgat cgtattgctt cccaccatat aagaaaccat
     6061 taccacaaca ccaagcatca aggaaagacc gataccggca agcaagtgca tatgctcgac
     6121 tcctttataa tttccttctt ctgcataatt gtctgcttta tggttcataa ttggaatcaa
     6181 aagccctaag taaatatttt tcaatatcaa tgtcaacgta gcaatcggca aacctagtaa
     6241 aagcgcggtt tctgtaccgg ctccagctgt aatagcaaaa gccgtcccta aaatcccacc
     6301 ggtcacaaca tcaggtggaa tcgaagcacc tactgagaac gatccgataa atgctaactc
     6361 taaagttgcg cccataatga tcccagtttt tacatcacct aacacgattc ctgtaaacag
     6421 ccccgtcaca atcggacgag aaagcaacga tgtccctagt gcatattctg attgtgcgac
     6481 aaatgcaaca attcctaata agactgcttg tacaatcatt gaggttcttc ctttcttaca
     6541 aaacttctct tacatccact tttttatctg ctggtacttg acggatttct acttctattt
     6601 gtttgtcaat taatcgtttc aagcgttcct cctcttctgg taaaatattt accgcttttc
     6661 cgatgctgcg agtaccaggt tttgccttga tccctcctaa gttgatggtt ttgatttctg
     6721 gatacgcttc tgccagctta tatgcatcct cgatcgattc cactacgatg aataacttat
     6781 atttatctgt caccccgctt ttcaacgcgg caatcgagtc atcaattgat ttgatcacta
     6841 gtttgactcc ttgtggcttg gctaatttga tcgttgtctt tcgtatctca ttcgtaggta
     6901 catcatcatt agcaatcaaa atacagtctg cgcctattgt ttgtgtccat gaaaaagcta
     6961 cttgtccgtg taataaccga tggtcgactc ttgtcaatac aatcattttt cttcctccta
     7021 aaattcgtct tcttctattt tctgattgag ccgctcgtta caaaactgga tcattttttt
     7081 tgaatcagtt agcacctgct ggatcaaaac agttgtctct acttcagcgt ccaaccgaga
     7141 tactagctca atcaatagtg gcaaattcaa tccggcaacg agatagacat gatcccgatt
     7201 gatatattgt aagaattcgt tgttgacact tccgccaaaa agatccgtca gaacaatgat
     7261 ttctttatct ttgtacctca taaaaagctg ccttactgtt tccgctaaat caaaattctc
     7321 atcagtatag caatcgaata cagtaataga atgatttttc ccatagatca gctctaatga
     7381 atctaatatc cctgaagcaa attttccatg ggaagctaag ataatttctc tatccacttt
     7441 ttcatctcct cttttcactt acacatacta aaaaagcaag ttgcatgcca aaattagtgg
     7501 caacaacttg cttatgttca aaattcttct tctatcgaga gaaactctgt atcttcactc
     7561 aaaatatcat agatataggc aatctcagaa ttggggatca tgacactata atgctcttca
     7621 atgacactaa acgcagattt aatagcagat aacccttctt tttgacactg cttccatttc
     7681 tctaatcctt gataggtttc gattggcatt tgtcggatca atcgttcaat gagacagctc
     7741 acatggacat acaacgctaa ttttttggga ttgctgatgc ttcgattcaa ctgcacctct
     7801 aattctctca taaaatgatc gatttctccc atcaccttct ctgtatctag aatagtcacg
     7861 gaatccagta ctttttccaa tgaaaaatta cgaatgatgc gctgattgaa tatttccttc
     7921 tctgaagcat tcatagtagt gtccaaccat tcagctagta cagacatctt ttcccccgag
     7981 atgaggtctt ctaatgaaag atagtcgata ttggtgatat caggatctgt ggtgccaatc
     8041 attcctacga cttcgtatat ggaaaataat gggtcactgt ttttcttatc ttttaattgc
     8101 tgatattcat atgggatgat tttcaactga caagccgtag gtaagctttt ttctagcagt
     8161 tcacttaagt ggaccgccgt accgattcca gtgaagcaag tagtaatgag tgctttgggt
     8221 ttgttttcct ctggatagag cagtcgccat tctggctgga tctgtcgaac agtctcttct
     8281 gctatctctt ctaatgatac atttttttgc aggcattccc ctactgtgat tgctaaaggt
     8341 gtcgtcacat tattcaaaat caaaagcggt acggataact gttttttaaa aaactgatgg
     8401 atttctttca gagatcccat gtctactaat atgatcaatc catttgaaac atcgctgttt
     8461 tcgcaataat caaggatttc tgaagcaatc tgctgtggtg tgacatctag tggcatatca
     8521 aatgattcaa atagatcctc ttgtaataaa cgatttacta catttgccat actgctagca
     8581 gttgcataac cgtgagcagc aatcatcgct tttggcagtc gtttttcttt tgaccatttc
     8641 gcttttttta gatagatcgt cagtaaaatc cgatccatac ttgtgtcttc aatatctaat
     8701 ttgatcttac ttaattccag aattcgttgg acatagtgat aactagatgg ataagactga
     8761 gaaacttgtt gttctaattc tttgattagt tggataatat catgttcttc cggtaaccat
     8821 ttgatagtgc ttcgctgata gagatagtaa ctcaaggcat agacactgtt tccatcgaat
     8881 ttgatttgat aggcagtttc catttgtcga aaggtctctc ggatagattg tgtccaataa
     8941 accagcagct cgtgtttttc ttgtctactg gtctcaaaca gcaaaaaatc aaataattga
     9001 atcacttctt gctttaattt tccttcgcaa gaatatagca gacagttatt tttcttgaaa
     9061 tcaattagga tttgctcaaa acttttaacg attcgctgct gttcgggctg ttgcttctcc
     9121 attaattgct ctaaggtctg gtcttgcgta atatagattc cttgctgtgc tgtattttta
     9181 gtaataggct gcgcaagtag ttctttttgc aaatgatgga tcgtgacatg gatttcaggc
     9241 tgttcacgct gttcagcata tgcttttgct gctgaaactt tgacgatatt tttcagctct
     9301 cctatgtttc cggataattt ctgacttgtt aaaagtgaca aagctggtcc actgactgac
     9361 aaagggctcc ccatttttct ttgttcttcg agaaaaaaag agtaaatcag ttctagccgt
     9421 tcattacgac tacgctgatt aagtgcaggt agttcgattc ggataggtat tcttcgaatg
     9481 aaagttgtta agaaatgact agtaatctct tcagtcgtag caaaaaagag acgtgttttg
     9541 atccggcgtg acaaattagg ttctcccata cgataaatca caccttgatc aagataggtg
     9601 aacagttttt cctgaccttc tggtgttaag cgatgtacct cgtctaaaaa caagatccca
     9661 ccatctgctg cttcaaaagc ccctttttta ttttcttctg caccagtgta cgctccctta
     9721 acatgtccaa acaaattgct agtcaaaagt tcaggattat ctgcatattg tgcacaatta
     9781 aaagtgatga atggtgcaga atcttctagc agatcatgaa gcagacaata ctgatagacc
     9841 aaattaacta aataactttt acctgtgcca ctttcgcctg taatcaaaag tggtaatccg
     9901 ccatctggat agtaaagagc tgttttgatt tgttcgatac tttctttcaa actggcgtcg
     9961 tatccgatca taaaagagaa taagtctttt ggttgcgcga aaaaaggctg ttcctctttg
    10021 agatcatcga tagaaaaata aaaattcgtg cgtaacttaa aaaattgttt ttcaaaagct
    10081 tctttgtgga aataaatcac tggtcgagaa ttgattttga ctagtagttg ctcatcattt
    10141 aactggttca aataatgact gactgtgttt ctttttaccc caaaatgatc agctaacctt
    10201 tgtgctgtaa agacctcgtt catatgttct aaatcgaaaa aggctgtctg gttctctaag
    10261 taacataaaa tttccttctt caacaactta ttcacccctc ctccatcata gaaaacggat
    10321 tcattttgtc aataaaatct ttaataaaga attgaaaaat atatcttatt atagacagca
    10381 gcctacttat cccttataat ttttattcta aaaaacgtac agttccttcc agtttttata
    10441 ctggaatttc aacatatgaa agaataagga ggctatgact attcaatgaa atcggataaa
    10501 tataaaaaca ggaaacatac ttcctggaaa acttttcgag aaatcatcgg atcttatctc
    10561 gctgtagttg tatttttgcc tttgctttat atggctatga cagaacaaac tcgttttatt
    10621 ggcgtagata cgcaatatat agccgaaaca ggatttttat tcatctttat cttgattttt
    10681 gcgttctata aatccttcca tacaaaataa gattcacaga tttcaacttt cctgtctgtt
    10741 tttagctatt acaaatcctt atatgtggta taacaacaat ttattggcat aatgaggaaa
    10801 aacgagttat gatgctttca gagagtaaag aggtactgtt gtcaccatcc tttttattga
    10861 ttacgtacac cactcttagt tttttcccat tgattacgaa aacaaggtag aaacattgat
    10921 gttcctacct tgttttcttt ttttattttc agatcaaaat ctcaagtagc ttttgacttg
    10981 atgtcgggag gttgtcttcg ttccatcaaa cctgatagaa tctgcgccaa accatagttt
    11041 tctccactat tatttattcc tctaagatag tacccacaat aaacgaataa atatttttct
    11101 cattttcata ctccttcttt gaaaatcaat tagatttctc tcaacccact tttttacaaa
    11161 aattacgtag caaaaaagat tgcaagaaca aattaaaaaa gaagtaaata gagtttcatc
    11221 tataaacatt tacttgatat attttttatg cagaattcat ttatagaaaa attaagtaaa
    11281 atagacgttc ctatgaatga agagactggc atgttttctc attttctttt ttttaaaaaa
    11341 aatagtctat tcaatttcta aagcttcttc ataataaaac tttcatttat gaaaaggctt
    11401 gttctctttt cattctaaat ttcctaagct acaatgtggt agtacttgtt ttttcaattg
    11461 tggacaaata aataatagga ggaatcatat gattcagtta ttacgttatg caaaagacta
    11521 tcgtaagcaa atcattttgg gcccgttctt caaatttctt gaagctgtct tcgaacttgt
    11581 cctgccttta atgatggctt ccttgatcga taatggacta aaaatgaatg accgaggaaa
    11641 aatcattgaa atgggcttat ggatggtcgc tatgtctgtg attggtctga tttgtgctat
    11701 catttgccaa tactatgctt cagtagcttc acaaggattt ggtacagagt tacgtaatca
    11761 gctaatcaaa aaaatcaata ctttctctca caacgagttg aatcattttg gaacagatac
    11821 cttgatcacc cgaatgacaa acgacatcaa tcagcttcag ctagctttag caatggtcat
    11881 tcgtttattg atcagagcac cgtttttaag tatcgggtca gtggtcatgg cttttgtgat
    11941 cgactgggaa gtaggtctgt ttttcttagc attattgcct attttctcaa tcattttatt
    12001 ttttatcatc aaaaaaaccg ttcctctcta ccaaaaagtg caagaaaaac tagatcagct
    12061 caatgaacac gttagtcaga atcttagcgg tgttcgtgtc atccgcgcat ttgcgcagac
    12121 aaaaaaagaa acagagaagt tcgataaaag cacaaatgat ctagcgaaca attatttacg
    12181 tgtttccaat atctctgccc tattatcacc tgctacaacc ttgatcatga atgtcgggat
    12241 catctgtttg ctgacagttg gcgggatcaa agtaaatatt ggtagcttgc aacaaggaca
    12301 agttcttgct ttgatcaatt atatgaatca aatgcttttg gcactgatcg ttgtttcaaa
    12361 cctcgtcgtg atcttcacac gtgccgaagc ttcaggaaat cgggtgaaag aggtgctaga
    12421 cacagaaaat tcgattcttg aagctgaggt cagtactact cccgattttt cttctgaagc
    12481 aattatcgct tttgaccatg ttgacttccg ttatactcct gattctggtc tttctctcca
    12541 aaatattaca tttcagctga agcgaaatac agtccttgga atcattggtc caaccggaag
    12601 cgggaaaacg actttgacac aattgattcc gcgtttctat gatgtcagtt ccggtacagt
    12661 tataatggac ggaatggatg ttcgttcttg gcagctggat catctccgtc agcaaattgc
    12721 tcttgttccc caaacttctg tactatttac cgggactatt cgtgaaaacc ttcaatgggg
    12781 taagaaagac gccacagaaa aagaatgttg ggaagctctg aaaatcgctc aggcagaaga
    12841 atttgtccgt cagcttccaa atggtttaga tacaaaagtc atggaaaatg ggaaaaattt
    12901 ctctggcgga caaaaacagc gactgacgat tgcaagagct ttgattgcca agccagcatt
    12961 actgatttta gatgactctt taagtgcgtt ggattatcaa acagacttgg atttgcgtaa
    13021 ggcgctacag acttatcttg gcagtacagt gattattatc tcccaacgtg tccgctctat
    13081 tcaagaagca catcatatct tagtgatgga tcaaggtaag attgctgctc agggtaccca
    13141 tgaagaatta cttgctcaat cagcagaata tcaggaaatc gtcgcatcac aggaggaaga
    13201 atagttatga aagtaaaaaa attatccttg cagacgctcc gccgattcat tccttacctt
    13261 actgcctatg caaaagacat ttggctagct gtgatactag gaatcatcaa tgggattgca
    13321 acagttgtga tgacgttaca aattggtcaa agtatcgatc aaatgattgg agaaggacag
    13381 gtccgcttca atcgactgtt ccatttactt attctattcg gtggtatcgt tttaatcaac
    13441 gtagtcagtc aatggctcat ccaagtgtta agcaatcggt tagcttactt atccgttgca
    13501 aaattaagaa aagatgcatt tgctcatctg aatcgattgc cacttcgtta ttatgatcaa
    13561 aactcccacg gaaatatcgt cagtcgattt acaaatgata tggacaatat ttccattgca
    13621 atttctgcgg ccttcaatca attattttct ggagtggcaa tcatcattct ttcgtttatt
    13681 tttatgatgc gtctaagtcc acttctgaca ttggtcgttc tttgttctac accaattatt
    13741 ttccttgtga gttggatcgt tgctcgtgct tcacaaacaa atttcgatag acagcaaagg
    13801 attattgggg atatctcggg ctttgtgacc gaacgaatcg gtaaccagaa aatcgtgaaa
    13861 gcttttcagc aagaagaagc tagtcaacag caatttgaac tgctaaatgc tgatctatat
    13921 gaaaaaggac aacgggcaca attttcttct tccttgacaa acccgtcttc tcgttttgta
    13981 gatcaccttg cttatttatc gattggttta gttggcggcc tattggcatt gaagagtggt
    14041 tcgtcaatca ctgttggtgt gatttccagc tttacgatct actcaagtca gttttctaaa
    14101 ccatttatcg aactttctgg aatcacgaca caactgcaaa ccgctttttc tggtctagaa
    14161 cgagcatttg atttgatcga tcagccagaa gaaaagcctg atttgccaga tgcctatgcc
    14221 ttggaaccca aaaatatcaa aggaaaagtc tcttttgatc atgtttcttt ttcttatgac
    14281 cccaagcagc gtttgatcga agatttcaat tttactgctt atccaggtca aaccattgct
    14341 atagtaggaa aaacaggggc cggaaaatcc acacttgtta atttgctgat gcgtttttat
    14401 gacacaacaa aaggaacaat tacagtcgat gaccatgaca tccgctctat ccaacgtgat
    14461 acattacgta aaagttttgg aatggtgtta caagatactt ggctatttga cagtacattg
    14521 cgagaaaatc ttcgttttgg gaatccggaa gcaagtgacg cagaaatcaa tgatgcccta
    14581 aaagcctctt atatgtatga atttgtagaa cgtttgccgg aaaaattaga tactaagatt
    14641 ggcggtcaag ggttaaagat atcagatgga caacgccaac tgttgacgat tgctcgaacg
    14701 atgatcagta atccccctat gttgatctta gatgaagcta ccagctcagt cgatacattg
    14761 acagagaaaa aaatccaaca agccttctta gcaatgatgg aaggaaaaac cagttttgtg
    14821 attgcccatc gcctgtccac aatcaaaaat gcggaccaga tattagtgat ggatcaggga
    14881 gctattgtcg aaatgggcac ccatgatgag ctgcttcaaa aagatggtta ttaccgacgg
    14941 ctctatcaag cacaattcac agcacaataa aaacaagcag tcttctggga tgggaagact
    15001 gcttgttttt
//
LOCUS       DACEUF010000063        14873 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.066, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000063 DACEUF010000000
VERSION     DACEUF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.066"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            149..838
                     /locus_tag="IXG20_001920"
     CDS             149..838
                     /locus_tag="IXG20_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610113.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            844..1800
                     /locus_tag="IXG20_001921"
     CDS             844..1800
                     /locus_tag="IXG20_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ1610114.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            1844..2356
                     /locus_tag="IXG20_001922"
     CDS             1844..2356
                     /locus_tag="IXG20_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1610115.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(2612..3160)
                     /locus_tag="IXG20_001923"
     CDS             complement(2612..3160)
                     /locus_tag="IXG20_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1610116.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(3249..4430)
                     /locus_tag="IXG20_001924"
     CDS             complement(3249..4430)
                     /locus_tag="IXG20_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ1610117.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(4666..6174)
                     /locus_tag="IXG20_001925"
     CDS             complement(4666..6174)
                     /locus_tag="IXG20_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ1610118.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            6333..6524
                     /locus_tag="IXG20_001926"
     CDS             6333..6524
                     /locus_tag="IXG20_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610119.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            6727..7644
                     /locus_tag="IXG20_001927"
     CDS             6727..7644
                     /locus_tag="IXG20_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ1610120.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            7637..8710
                     /locus_tag="IXG20_001928"
     CDS             7637..8710
                     /locus_tag="IXG20_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610121.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            8858..9376
                     /locus_tag="IXG20_001929"
     CDS             8858..9376
                     /locus_tag="IXG20_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610122.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            9357..9749
                     /locus_tag="IXG20_001930"
     CDS             9357..9749
                     /locus_tag="IXG20_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ1610123.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            9825..10760
                     /locus_tag="IXG20_001931"
     CDS             9825..10760
                     /locus_tag="IXG20_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ1610124.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            10893..11672
                     /locus_tag="IXG20_001932"
     CDS             10893..11672
                     /locus_tag="IXG20_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ1610125.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            11665..11982
                     /locus_tag="IXG20_001933"
     CDS             11665..11982
                     /locus_tag="IXG20_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1610126.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            12226..13836
                     /locus_tag="IXG20_001934"
     CDS             12226..13836
                     /locus_tag="IXG20_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1610127.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            13993..14841
                     /locus_tag="IXG20_001935"
     CDS             13993..14841
                     /locus_tag="IXG20_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1610128.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         4803 a         2663 c         2926 g         4481 t
ORIGIN      
        1 gttctatcca tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg gcaccatttc
       61 tattttttgt ttcttgtata ttctgcctag tattttaaaa ccatttcatg taaaatgtaa
      121 atcgaaaata tgttcggagg tagaaaacat gaagaccccg ctaaccatcg aaatagaatc
      181 ctcgttatac caatattgca tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc
      241 tgatgaccaa ggaattgtag atacgcttag ctgcttgaca aaatttgatg gaagccgaga
      301 atggcgatgc tatgagctga aagtgacaaa agcagatttt cgctctacag ccaaactgtc
      361 atttgttgga cattataatt attttgtttt accaagtaaa ctatatgaag aagtaaaaac
      421 tgaaatccct acaggaatag gtgttttagt gtatcgtccg tacacggttg aagaggagat
      481 gcctgcctct ggtacatttc tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc
      541 ggaagaagct ttgacaaacc gcttcatggc ttctttattc agggaagtaa gaaaagcaaa
      601 gcaaatggag cgaggcgtaa aatattttcc aaaagatcaa ttgtacaaag aattgaaaag
      661 acgttcagaa aatcaagatc cattttctaa cgaacgatat tatgaacggt ttttagagga
      721 aatagctagt caggcaatca atgagttgca agaagaagtt atcgcgcttc agcatgatta
      781 tgaatatttg aggcaacaac agcagatccg tcggttgcct acagagccat tggagtgaaa
      841 aatatgtact atccttattt aagaggaaaa caatttgacc tattagcatt aaaagaagca
      901 ctgtcgcgag gattgctctc caataaaatc caaccagtga tcgaaccagt acgtgattcc
      961 gctacgctta aaaatgtgat cgaattattt caaaaaaagc accatccaat cgctgtgatc
     1021 caaaatcccc aagtaggaca attcaaatta ttcgatcagc atgtgcacag ttggacagtc
     1081 aaagaaaata gctctgtcgt ccctgcacag atcttaacgc cagaaaatct cagtgaagta
     1141 ctggattcac ctccagcgtt tcttgttttc gatggacagc actacccaaa agatacagag
     1201 gtgtggaaac agttggcagg agttgattcg aaatttttga taccagatac tagtcgcttt
     1261 cgcatctggc tgcccgaaaa caaaattgtg atccgtgact cttttcaaac gagaaagcat
     1321 gttgaaagtt atgcagataa aacagatgac ttttttagtg atgactatct ttttttccac
     1381 gcagacggat atattggatt ttctgatttc acgatcgaag gtagccgtta tttcgataaa
     1441 gggttcccta gccgagcgct tgctatccat ctaacgtata ttgatgctta cggcaatata
     1501 cgcgtgaaac attttgtctc tgacagtaac gactctgcta aagaccaagc attgaagttt
     1561 cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc accatcatca actcttgatt
     1621 acttcaggaa tgattgaatt attggctctt taccagcaac aaaaattccc tggactggga
     1681 gtgttgaaaa agtggtcact tatgcatcat ttggaactta ttagtcacct gctagatcat
     1741 ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac tatatgaagt gatccaatga
     1801 ttagataatg taaatgaaaa aggaatagaa aagaggaaac tggatgtata ttcggcaaac
     1861 aaggaaagaa gaagtagaag aagtggtaga aatcattaat gccgcagcgg atttcattgc
     1921 ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa
     1981 cgatattgaa agaaaagaaa gctatgtatt aatagtggaa gatcaaattg ctggaacagc
     2041 tgcattagtc tcaggcgtgg atcccgttta cacaaaaata gatggtagtt ggaaagaatc
     2101 aactggtcct tatttatcga ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa
     2161 actagcaaaa ccgttcttag aacatttgtt gacgattgca gccagcaaaa atatccaaga
     2221 tatccgtatc gatacttatc cagctaatga accgatggaa aagaccatct attctgctgg
     2281 attcacttat caagggatga ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca
     2341 aataatcatc aattgactat aaaatcatct gagcaaagat aaagacatta acactactga
     2401 aaaaaacaat catatgatag ccaagaggtt gtaacagaag tggccagctt caaaaaacaa
     2461 gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat tttacgaaat
     2521 agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt cacgatctct
     2581 cggcttatcc tacgagtatt attcgataca tttatatcaa taatcctttt tttattttat
     2641 ctgcagcttc ttggaacgct ccatcgtttg acacgactaa aagtgagggg atagctttca
     2701 aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag tctggctgaa atctgttcag
     2761 atgaatctcc acgttttttc aggcgcgatt gcaatgtttc tttagagatc gtaataaaga
     2821 taggacggac taccgtcccg aaatactcat ataaatgcca aaaaccattt gctgtcaaaa
     2881 cggcgtaaca atttcctact gtcagtttct ttttaatttc tgctttactg gaaccatatt
     2941 tatgttgagc atattggtcc cactctgcaa actcatttcc cttgatcatc tgattgaatg
     3001 tattgtcaga aacaaaataa taatcctctt gatctttttc cccaggtctt ggtgatcgat
     3061 tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc ttctgcgagt gaagtttttc
     3121 cagaaccaga aggaccgata aatacatagc aataattcat tgtcatttct cctttgatga
     3181 ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct tttaaaagta actttgcttt
     3241 tttgtatttt ataccagcaa gccagtcaca atagcactta aaaagctgac taaagtcgct
     3301 gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc ctttttcttc atttaatccc
     3361 ttcatcgcac cagaaatgat cccgatagaa gagaagttgg caaatgaaac gaggaagaca
     3421 gatataattg ctgttgtacg ctcagtaaaa tggaaattac catttgctaa ttctgtcata
     3481 gcaacaaatt catttgtaac cagtttagtc gccatgatac taccggcatc cactgcttct
     3541 ttccatggga ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg
     3601 aaagtgatgc cgaatatccc gtggaagata gcattgatca tggcaatcaa agcaacaaaa
     3661 ccgataagca tagcagcaac cgtgatcgcc acatggaaac catcaagaat gtattctccc
     3721 aacatttgga aaaatgtctg ttctttgttt tcttcaatga tcagctcatc ttctttttct
     3781 tctaatacat agggatttac aattgaagcc agaatataac cgccaaataa gttcaaaaca
     3841 agggcagtga tcacatattt agaatcgatc agtgccatat aagatccgac gatcgacata
     3901 gaaactgtag acatagccga tacactcatg gtgaacaaac gtttctcaga taggaagggt
     3961 aattcctttt ttatggaaat aaaaacttcc gattgtccta aaatagcaga agctactcca
     4021 ttataagatt ccaatttgcc catcccgttg attttactta atcctagacc aacagctttc
     4081 ataaataatg gaagaatctt gatatagcgt agaataccga taatagcaga aataaataca
     4141 attggcatca atacggaaag aaagaaaggt gcacttcctt tatttgctac accggcaaaa
     4201 acaaagtcaa caccttgccc agcaaaagca agaagatgat caaacatttt tgccaataca
     4261 gaaacaaccg ctgtaccaaa agttgtacgt aataatacga aaccaagaac aagctgtaaa
     4321 acgaacatca caattagtgg tttcaatttg attgcttttt tgttactgct gatcaaaaaa
     4381 gcaagcccta atgtgacaaa taaaccaata atacctaata aataactcaa ataatcactc
     4441 ctattccaaa ttccgtttta ttttacctgt tttgcaaata aaatactaat ctttagtgaa
     4501 attttcatta cttttttaga gtgaaacgca ttcattttcc attagaaaat aaagcgaaca
     4561 aaataaaagg tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg
     4621 attaagctaa tagtttcgtt ttcaacattt ataagtaaac tttttctatt ggtaatatag
     4681 attttcggaa gggtagacag gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg
     4741 ttcatcattt ttattgacca tcacagtaga gtgggcttgt gctccctcaa gttcagctag
     4801 tttatcataa gcaacttgag cgattggatt cgttactgca ctgattgata aagcgatcaa
     4861 gacttcattg atgcttagtg catccaaaga ggaatgaagt gtctttgatt tcaatccttg
     4921 tattgtttca agaactaatg gtgacaataa aggaatgtca tctgcgatat gggctaaatg
     4981 ttttactgca tttaagataa ctgccgctga cgcatccatc aatgaacttg tacgcccggt
     5041 aatgatgact tcatctggta attcgattgc aatcacagca gaaggatctt ttgtaggact
     5101 ttgcgcttga atcttatctg catagtcatg ggcagggact accacgtttc ggtcttcagg
     5161 acgtaaaccg acttcttcca taatcagttt gattcgatta actgcttctt cgtcgatcaa
     5221 tccttttttg aagtcgcatt ctgtttggaa ataacgacgg atgatttctt gtttagaggc
     5281 ttcttggaca acggtatcat ccacgatccc aaatcctaca cgattcacac ccatgtcagt
     5341 aggagattga taaacagatt ctttaccagt aattttttca ataatacgtt tgattacagg
     5401 aaatgtctca acatcccgat tatagttcac agctacttta ttgtaggcat caaagtggaa
     5461 ggaatcgatc atattgacat cttttagatc gactgtggct gcttcataag caatgttcag
     5521 cggatgtttc aacggaacat tccatactgg gaaagtttcg aacttggagt aaccagctgc
     5581 atgtcctttt tggctttcat gatacagttg gttcaagcaa gtagcgagct ttccacttcc
     5641 tgggccagga gctgtcacta caacgattgg tttagttgtt tcaatatact cattttttcc
     5701 aaaaccatct tctgagacga ttttttctac gttgactgga tagtcttcga tttctgagtg
     5761 tttatagacc ttgatgtttc gtctttctag tttattgata aagactttcg tggcaggctg
     5821 tccactatag cgagtgataa cgacactgtt gattaataag ccataaccac gtaattcatc
     5881 gattaaccgc aagatatcca tatcataagt aatcccataa tcaccacgga tcttattacg
     5941 ttcaatatct ccagcataga cgcaaatcag tatttcagct tgatctttca atttttgcag
     6001 caatttgatt ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc
     6061 aactaacttc ccaccaaatt ctaaatataa tttgtcatag tggttcactc gttccaaaat
     6121 atacgctgac tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt tcactaggtt
     6181 ccctccttaa gtactaaagc attttttcat ttttttgagg attcgtcaac tggaagagcg
     6241 aaattcctca aaaaaacaaa gaaaatggtt taaaccaact tgtctagata gttattcatt
     6301 ggtatgatag tttttgataa ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg
     6361 catataaaca ctatatccgt tcgattttta ccggttatga aagaatgctt gaagatcccg
     6421 aattggaaca attgacgttt acttatctaa acgaagaaac gcagctaact cgtgatgagc
     6481 atcagcgcat tcatttcaca acaagagatc tgcctagtaa ataaaaaata agataaaggt
     6541 catcggattc gattctgtcc tatcagatgg aaagactagt tgatcgaaaa aaaggctgta
     6601 ccaaattttg gtgcagcctg ttttatggag cattgcccgc tcttttttat aacaattttt
     6661 tacagaaaat ggtactattg aagtattaat aagtgaaaaa cagatgagta agaggaggca
     6721 agtagtatga aagagccaga tacaaatgca aattatgagc cagacgaact agaggagacg
     6781 aacgcggatc aggatacagg acaagaatcg tcttcttttt acgatatcaa agaaaaagca
     6841 agcatgaatc tttcccaatt attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa
     6901 gaagatatcc ctgagatcta ccgtttatac aatggccgat tgaatgaaga attaaaaaga
     6961 agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt
     7021 ttacaggaat ttccttttat ggaacaagta gaaaacatct cgccaacgat gagctattac
     7081 aaaatcggca cttattacca tgatcggccc acggtcggta ttgatgcaag tctgccagaa
     7141 atttttgttt tgcctaagat cgatgaagag tgggagaaat attcccaacc ggagtcaacg
     7201 gactatgaca aaaaaatcaa tgagctagac gcaaaggtga tcactgctca gacagaaatt
     7261 gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa tgactgattt gaacgacaat
     7321 aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga gaagaagaag
     7381 caagtactat ctaatgaaaa attaggctgg cttccttata ttgagacgcc tgaaacaatc
     7441 cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag accagcttcg tgcagccatc
     7501 gtagaaaaag aacaaaggca aatcagacgc tatttcggta gcaaagaggg attcggtcaa
     7561 gcaatccatg aattcttgat gaattatctt ggcaatgaga atcctaataa tcaatctaat
     7621 gaaggaggtt ccgagtatga atgaacacga tgaagcagaa tggacaaaaa atgaagaaca
     7681 aacagaagat catgaagaaa cgaacacttc attggaagaa caaacaagta atcctctgga
     7741 tgacacagaa gaaatcccta tgctagagga aacaaaaata catgaaaaag aaaaagtaac
     7801 tccgacgaat acgaatactg cagaaaacac ccatgcttac gaaataactg aaaatcccgg
     7861 gtatttagaa ggaaaagagc aagatcatca agatatcgtc agtttgattg aaacagcgga
     7921 agcagaatta gcggatttac gtatgagaaa agatttgatc gaatcagatt ttccacaatt
     7981 attagaatca tatgatcgtt ttatattgaa tgaagaacca atctccacga tcggaaagac
     8041 aagcctgatc gaatacttca cgtatgaatt actgaaaccg ttgaatgata ttggtctgga
     8101 acggtcaaat tcagcttatg acacatggaa aacaagacat ttttcgatca attatacgtt
     8161 gaatggacaa aacgaattag tcttttcctt tgtattaccg accatcgatc aacgatacca
     8221 agtggaatcg atgagtctcc taaacgtatc accagagaca atggaaatca atgtccaaga
     8281 tgaccgggtg ctttctttga tccgacactg gtcagtggat cgagtattct ctgcagggca
     8341 gatcacgata ttcaatcaca aattgaatca attattagcc catgcaagaa cacttggttt
     8401 taccgttaat cagacattac tagacaatac gaagccactt cgtttgaatc ttcagagcga
     8461 atttgaattg acgaatgaag tattggatga tatttttatc gtggcaatga aaaatccatc
     8521 ctacgatatg gaaaagatca acgaaactac ttatcaagta cgattagata aaggacagtc
     8581 tttaacgata acaacaaacg aaaaagatca aacagtcttg tcgatctcat ctggagacta
     8641 cccacgatct gtcattgatt tctttatcaa ttacgaattc ttggtcccat taatggtgag
     8701 aaagtcctga ttggataagt tggattacca gatgtcattc agataattct gttccaaagg
     8761 acttgcggca gttatccaga ataattgtca caagtctttt ctgtatgtac taaatggacg
     8821 tgaataactt gctgatgaag gagggatttc attaggaatg tccacaaata aaatagcaca
     8881 gcttagctta ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat ttattccgaa
     8941 tttccagcca atgactgcta tttttttatt tatagtactt tacttaagct taaaagatgc
     9001 gttaattgtc atgagtcttt ctattgcgat cagcagtttt tattttggtg ttggaccatg
     9061 ggtcatcggc caatggatca gctatactgt tgttctgagt ttgttttcaa tcgtctgctt
     9121 gagaaaaaca gtgcaaagga atctatggtt aaagggagtc tgtttttttc tggcgggcat
     9181 ggtttacgga ataggtatga ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg
     9241 gatctattac cttcaagggg tgtcgtttga tttgatgcac agtataggca atgttgtgtt
     9301 ctttcttgtt tttctgccag tcgtcaaacg tttttacaat cattcaggag gaaaaaatga
     9361 aaaaaataat ttatagtttg cttgcaatga gtgtcagtat actcattttg acaagttgca
     9421 gtacaaccta tacacaatcg gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag
     9481 caacaatcac gttacaagaa aatggtcaaa acttttcgga aaaagaagtg gtcttcaaaa
     9541 aaggcgatga tttactgagt attttgaaac gaaattttga agtcaaagaa gataacggct
     9601 tcatcacttc gcttgaaggg cactcacagg atgagaaaaa tcaactctat tggatgttta
     9661 ctgtggacgg gaaaagtgct actactggtg ccaaagagat caaattgaat gatggacaat
     9721 cggtcatttt caatttgtct aaactataag tttcataaaa aactgttaaa gggcgaatat
     9781 tgccataatt cagtgagttc agttgatgaa agaaggaaaa taacatggaa aattttaaaa
     9841 aaagcggaat gttttccgta attgctgctc taggggctaa tattctagtt gctatcagca
     9901 aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc atccacagta
     9961 tcgttgattg cagcaatcag attttgctgt tattcggtga taaacaagcc aataaaggac
    10021 agagcgagtt gcatcaattt ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg
    10081 caatgatgct tttcttcggc ggtggggcac ttggggtgat ggaagctttt gaaaagttga
    10141 cgcatccatc ccatgaagtg ggtaatccga tcattgttat agcaatactg ttatttggtt
    10201 tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg
    10261 aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata ctgattattt
    10321 ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc tttagcagga acaatattga
    10381 cttatgcaac cgggattgct gcatttgatg cgatcagcgg attgctgatc ggtttcttgc
    10441 tgatgggagc cgccattttc ctagcaaaag aattttatag tctgatcatc ggtgaaagcg
    10501 tgacagcaag cgatttatca aaaatcaagt tagcttttga acgtcctgag atcagtcggc
    10561 tgatcgatat aaaaacagtt catttaagtc caacggagat tttagttgca gcaaaagcag
    10621 atatcgttgg taacaaagag aatgaagctt actcggtcat caatgatatt gaaaaaaata
    10681 tgcgtcagtc tcttcctgac aagaagatgt atatatacat tgaaacagat aaatatgacc
    10741 caaattattc tcgtaaataa aacgtttatt catgaattga agcaaaaatg agaaaaatat
    10801 aatcttgttt ttaaataaaa cggctacaat ttagcgaatt tatgttacaa tgagtccgct
    10861 gtttatttgt tgtaattaaa ggagatattt taatgaacaa aaagaaaagt gcgccatggc
    10921 ttcaagcttt gcatgcagtg atgccacttt gtataagcta tataccagta ggattagctt
    10981 gcggagtatt gttgcaaaaa gttggatttg atcctctctt atcaggactg ttatcgattc
    11041 tggtcttttc cggtggcgcg cagtttttag ttgcatctat gcttacgacg caagcatcat
    11101 ttgcaacgac attattgatg gttttctttt tagaattacg ctatacatta ttaggagcaa
    11161 gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac
    11221 tgaatgatga aaactatgcg gtgaactact taaagttttc aactgaccct tcctggaata
    11281 aaagaaaggc tttatatgtc aattggttct caatgactgc atgggccggt agcaatatga
    11341 tcggtaattt tttcggctcg gtgattcgag tagatgctga ccttgtccat tttgctttga
    11401 cggccatgtt cattttcatg tttatcatgc agatgaaaag cgcactactg atctttacag
    11461 gtttattctc aggcgtatta ggggtcgtat tcatggtatt attccaaaat actttcggat
    11521 tgattgcagc aacagtcatt gcttcatttg ccggatttat gctggagaag gcattcaaaa
    11581 aagaaaaagc taagaaacta agagaccgcg gtaaagatgt caatgagccg ctcttcaact
    11641 tcccggaaca ggaggccaat caccatgact aaactatatt tgctactttt agttgcttgt
    11701 ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa
    11761 gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta
    11821 gcttttaaag atgttttcat tactcatgaa catctggata ttgcttggaa tatcaaaata
    11881 gctgcaatga ttctagtagc aggcgtggct tacaaaacac gttcgatggc gttgtcagtt
    11941 ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt attttattaa
    12001 aaacagagat tgcgacataa gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt
    12061 caattctgga tgcaagttta ttcgtaggga taaaaaagat tagtgttggc ttatgatgca
    12121 agctcttttt tattttagga attagagagg gatttgtcct tctatgattt tttgctataa
    12181 tagggaagat gtttatttta tgaaagaata gttgagggaa tttaaatgaa taaaaaattg
    12241 atgcaaggaa cattttggct aacctttgct aatcttctat gtaaagtttt aggggtagtc
    12301 tatttgattc cctggctaag tatgatgggg aacaatcaag atggtatgct tgctacgaca
    12361 ttgtataatg ttggctatct tccttatgga ttgttcctga tgcttggtac agtcggattt
    12421 ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag aaggagataa cgctgcttgt
    12481 cggctgattt ttagaagtac gatcaatatc atgtttgtca tcggtatcgc ctctgctgca
    12541 ctgatgtatt tatttgctcc tttactagcc ggaatcagtc cgattgctaa cgttaataat
    12601 gggatactag ctatcagaag tttatgccca tctttgattg ctatcccaat tcttagtgca
    12661 atgagaggat atttccaagg aaaaaatgat ctgcggcctt atggaacttc tttgattatc
    12721 gaacaggctg tgcgagtgat cgttatcttg gctggtactt attacttgcg cgtattgact
    12781 gacggaacca tattagaagc tgtattgatc agtacagtcg cttctttctt cggtgggtta
    12841 gctgcgatta tccatatgta tatcgtcgga cgcagaaaag acttcttcga acttaaagat
    12901 tttttgattt ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt
    12961 cgggaaacac taccgtttat ttttgtcggc tcagtcatta ctatcgttca aatgatcgac
    13021 caagtaacga tgaaaccact gcttcacttt ttccgtccag agatcgcaga tcagcaactg
    13081 gaattcttat tcagccgtgc ttctgtcaat ccaaataaat tgacattgat cctgatctcc
    13141 atggtaggaa cagtagctat aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat
    13201 cggatacaaa tcgaaaaaac agtaggagat agtttttcga ttgcgttatt gattcttctt
    13261 ccttcattga ctggaatgtc tttattagct ggaccactat acacattgtt ttttggctat
    13321 gatcctgaaa gtgtcggata tttccaaatg gctttgctag cttctttgtt tttctcttta
    13381 ttcacgatcc tatctacgat gcttcaatct ttgaatcacc atcgttttgc gatccgatta
    13441 actgtagaag caattatcct gaaagtcatc ttccaagtaa tcggattagg attagttggc
    13501 ggatacggga tgagcttatc aaatggctta gcattcggta ttgtctttgt tcgcggctat
    13561 cactttatgt gcaaagaata ccgaatagca cctttagcaa agatcagcaa ttttttctta
    13621 aaaactttcc gctccaccct gattatgctt gcagtatgtt ttgccgtttt ctttttgctg
    13681 aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt ggctgtagga
    13741 agtattggcg gcttagtatt tttattttct caattcggaa aaaatgggtt tcgaatgttg
    13801 aaaaatgtta aacatcatag cagaagcaaa gaataggtta actaatcgtt ttttgctgtc
    13861 acttgaaaaa cggcaactcg tatgattacg cttgtcgttt ttctattttc caattttagt
    13921 ttcatatcta atcttactta tgatatagtt ataaggtctg gaaaaattct ttgaaacgag
    13981 gaaattaaac acatgaaact tgcagtagtt acagatagtt cggcatatct gccggaacgt
    14041 atcaagaacc atcccgactt attcgttatt ccaatccctt tgattatgga tgggaaaatt
    14101 tataacgaag gaatcgatat cgaagcggat gaatattatg gacttttaaa aaacagtaaa
    14161 gattttccta ctacttcaca acctgtcgta ggggaagttt tgggtttata tgaggaattg
    14221 aaagagaaag ggtatgacac gattttaagt atccatcttt cttcagggat ttctggcttt
    14281 gtcaacacat tatttgcgat gaaagatgac atcagcggag taacggtcat tccttatgac
    14341 tctaagatca ctagtatgcc gatgggacac atggtagaag ctgccttaga cttaaatgat
    14401 gaaggtcagt cccttgatga aatacttgca catatcgatc gaatccgaga taatacctat
    14461 gcttatctga ttgtagatga tttgaacaat ttagtacgag gaggacgttt gacaaatggt
    14521 gcagcgctga ttggcagctt attaaaaatc aaaccgatcc tgacattttc tgaagggaaa
    14581 attgttttat tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt
    14641 attggtagaa gaaataacga aatacaaccg ccagtcaaac tttacgtgat acatgcaaat
    14701 aatttagaag tagcgattgc ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa
    14761 atcgaaattg gtcatttcgg accagtcatt ggtacacatt taggtgaaaa agccatcggc
    14821 attgccattt cagcgcaata attcctttta ttcaaaaaac ataccaaaaa tat
//
LOCUS       DACEUF010000064        14574 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.067, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000064 DACEUF010000000
VERSION     DACEUF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.067"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(<1..141)
                     /locus_tag="IXG20_001936"
     CDS             complement(<1..141)
                     /locus_tag="IXG20_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1610129.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYC"
     gene            414..2885
                     /locus_tag="IXG20_001937"
     CDS             414..2885
                     /locus_tag="IXG20_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1610130.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2988..4724)
                     /locus_tag="IXG20_001938"
     CDS             complement(2988..4724)
                     /locus_tag="IXG20_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1610131.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4721..5425)
                     /locus_tag="IXG20_001939"
     CDS             complement(4721..5425)
                     /locus_tag="IXG20_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1610132.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5556..6059
                     /locus_tag="IXG20_001940"
     CDS             5556..6059
                     /locus_tag="IXG20_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ1610133.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6019..6357
                     /locus_tag="IXG20_001941"
     CDS             6019..6357
                     /locus_tag="IXG20_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ1610134.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6378..7796
                     /gene="ffh"
                     /locus_tag="IXG20_001942"
     CDS             6378..7796
                     /gene="ffh"
                     /locus_tag="IXG20_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ1610135.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7908..8486
                     /locus_tag="IXG20_001943"
     CDS             7908..8486
                     /locus_tag="IXG20_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ1610136.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8490..9011
                     /locus_tag="IXG20_001944"
     CDS             8490..9011
                     /locus_tag="IXG20_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1610137.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9090..9644)
                     /locus_tag="IXG20_001945"
     CDS             complement(9090..9644)
                     /locus_tag="IXG20_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1610138.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9897..10172
                     /gene="rpsP"
                     /locus_tag="IXG20_001946"
     CDS             9897..10172
                     /gene="rpsP"
                     /locus_tag="IXG20_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ1610139.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10184..10435
                     /locus_tag="IXG20_001947"
     CDS             10184..10435
                     /locus_tag="IXG20_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1610140.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10560..11351
                     /locus_tag="IXG20_001948"
     CDS             10560..11351
                     /locus_tag="IXG20_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610141.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11521..12042
                     /gene="rimM"
                     /locus_tag="IXG20_001949"
     CDS             11521..12042
                     /gene="rimM"
                     /locus_tag="IXG20_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ1610142.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12032..12793
                     /gene="trmD"
                     /locus_tag="IXG20_001950"
     CDS             12032..12793
                     /gene="trmD"
                     /locus_tag="IXG20_001950"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ1610143.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12929..13276
                     /gene="rplS"
                     /locus_tag="IXG20_001951"
     CDS             12929..13276
                     /gene="rplS"
                     /locus_tag="IXG20_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ1610144.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13534..13827
                     /locus_tag="IXG20_001952"
     CDS             13534..13827
                     /locus_tag="IXG20_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ1610145.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         4886 a         2617 c         2949 g         4122 t
ORIGIN      
        1 acaataggaa ggagagtcat ccaattttcc tttaataaac tgcgcttgaa cgcctttata
       61 ttctttttca atcagccatt cttcatcaaa aaataaatgt ttatctgtta atccgagtaa
      121 ttttctagta tgattatcca ttgagagact cctttggtag gatgacgttt tggtcgacta
      181 aattttacca aaagtgagtc tctttttgag ttgaaacgaa gaaaaagtgt tagtagaaaa
      241 tcgccttcga ttttccacca acacaaaata ttatagaacc cttttttttt gctatatgac
      301 aggaatgaaa ggtgtttgca agcttaatgt gaggaacact ttccatatct gttctatact
      361 ggataggaga agtttggaaa tatccagaaa acgaaatcaa tcgaggtagc cacatgaaga
      421 ataaatggat aaatcgaagt aaaaaaaaga aagtaacaca aagatcatca gataaagtca
      481 tccgtctttt tccatcgtat aaagaaggat taacggcaga tcaagtcaag gaacgaatag
      541 aaaaaggtgc agcaaataat tcagtcgatc ccacattcaa aacaaatcag caaatcgtat
      601 tggaaaacat atttacgtat ttcaatttga tctttcttat cttagccatt ttgctttgtt
      661 tagtagaatc ctataagaac ttgacttttt taccagttat catagccaat acaggaatcg
      721 ccatttttca ggaaattcga tccaaaaaga tcttggacga actaaatgtt ttaaatacag
      781 caaaagtcaa tgtcatacga gacgggatag aacaaacaat agatatagaa gaccttgtaa
      841 tagatgacat tgttatttta gagactggat atcaaattcc agcagatgca gaggtcgtgg
      901 aagggaaact gcaagttaat gaggctttat tgacaggcga agcagatgaa ataaccaaag
      961 aaataggtga tccattaatg tccggcagtt tcattgtttc cggaaaagct tacgcccgat
     1021 tagacaaagt tggagcagat tcgtatattt cccagctgac agtcaaagcc aagacaatgg
     1081 gagaaggaga acagtcggag atgattgctt ctcttaatca attgatcaaa tggattggga
     1141 tcatcattat tcctatcggt atcctgcttt tttctcaaag ttactttttt aatggaaaca
     1201 cgatcaaaga aagtgtcgtt gcaatggaag cagcattgat cggtatgata cctgaaggac
     1261 tgtacttgct gacgacgatt gcattagcat taagtgcgac taaactagca aagcaaagag
     1321 ttcttttaca caatatgaaa agcattgaga cattggcaag ggtaaatgtc ctttgtgtag
     1381 ataaaacagg aacgatcaca gaaaataaaa tgtctgttca aaaaatgatc gtctctaaaa
     1441 aacaagaaat aatcagtcag tcagatcaat tggaagcatg cattgctgac tatgcaaaag
     1501 cgatggcagg agacaatgca acaatggaag ctgttaaagc atattttaca aaaaccactg
     1561 atcaatccta tcaccatgtt attccttttt cttctgtcca aaaattcagc agtgtagcgt
     1621 tggcagacaa ggtgtatgtt ttaggagcgc ccgaaatggt tttgcgcgat caattgccgg
     1681 aatatgcgga agagtttgtt tctttcgctg aaaaaggcta tcgcgtgttg gtctttggtg
     1741 tgtacgatgg gattcttgaa gaaccagtat tgacagaagc agtcacacca ttaggctata
     1801 ttctgattgc taatcctatc agaaaagaag caagagccac atttgattac ttcaaaaaac
     1861 agcaagtagc catcaaagtg atttctggag ataatccgct caccgtatcc aatgtagctg
     1921 tccaagcagg gatttcacat gctgatcagt acgttgatgc atctaaatta tcagaagaag
     1981 aatatgaagc agctgtcgaa aaatatacag tttttggacg cgtcaagcca gaacaaaaga
     2041 aaatcttcgt tcaattgata aagcgtaaca atacagtagc tatgacaggt gatggcgtga
     2101 atgatattct agccatgaaa gaagcagact gcagtatagc gatggcttcc ggaaatgaag
     2161 ccgccatgca ggcctcacaa gtggtcttac ttgattctga cttttcacgc atgccagaag
     2221 ttgttgcaga aggacggcgc gttgtgaaca atattgagcg atcagccagc ttgtttttag
     2281 tgaagaatat tttctccttt ttgctctcac tgttttcagt gatctttgct ctgacctatc
     2341 ctttagaacc atcacaaatc acattgatta gtttgtttac tatcggactt ccatctttct
     2401 tgcttgctct agaagaaaac aaaaagcgaa tcagagggaa attcatcatg aatgtgatgg
     2461 aaaaagctgt gccaggtgga ctaacagata tgatcgtagt cggtgcgttg gtgatctgcg
     2521 gggtgacttt gaatctaaat aaaacagatg tttctacagc atctaccatg cttttgattg
     2581 cagtagggtt cctcgttctc tataaaatct gttcaccgct gaataaattc agaagtcaga
     2641 tcattctttt ctgtgctagc ggtaactttt tttcggtcat ctttttgcat aagctgtttt
     2701 caatcaccgg tatctcagct gtatcacttt tgctgttgag cattctattt ttctcggcag
     2761 attcgatttt taggcatttg accacattgg tagaatattt gtttgaacgt aaaaatgaaa
     2821 aagagcaaaa gccgtttagt cgcgtagcta aaaaatttct atcgatattt aaacgagaaa
     2881 attgacgaaa agtatctcaa aaaagagtaa aaaagaacaa ccactcgtgt atctttcgaa
     2941 gcaggatgag tcatccaact ttaaaacgca cgagcagttg ctctttttta cattttcgga
     3001 atggtaacgg tgaaagtcga acctgttcca agatgactgt ctacagtaat cgtaccatta
     3061 aggagttgca cgtagtcttt tacaatcgct aaccctaatc cggtcccgcc tgaatgtcgg
     3121 cttcgcgcct tgtctacgcg gtaaaaacgc tcaaagatcc gttcctgatc ctcaatgtcg
     3181 atacctatac caaagtcttg gacagaaaac gatagatcat ctgttgcctg atagcggata
     3241 ataatctcgc tgtcgctttt tgaatattgg acagcatttt caatcagatt tttgataatt
     3301 ggataaagga tatctgtttt agtagtaaat ggttcgttct ttccaatcaa tcgaatcgtc
     3361 aaccgtttct tttcgatgat tggctgataa ttttgaacga tttgctggaa ataaggttct
     3421 gtataaattg tctggatctc gtaagaattc tcgccatctt ttgacagctg gatgatttcc
     3481 cgaatcagtt tatctaaccg gatcgcatct ttttgcatga tctcaagaaa agaagtcaga
     3541 gtttgcgggt catcttttgc accatccagt aaagtctcgg taaaaccaat cagtgaagtc
     3601 acaggagttt ttaattcgtg agagacgttg ccaacgaaat ccttttgtag cttttctaaa
     3661 cgtcgcactt tcgttaagtc atacgccacc cctagaatct gtccggtacc ttctgaatta
     3721 gtaaagtagc gcaaactcaa gtctaatgtc tgccgacctt ctgtaagggt gatctcctga
     3781 tggatcagcg gatgctcagg agtcacttga tgaatcagct gaatgagttt tggctcttga
     3841 atgacttctg tgtaccgctg ttcaggttga tagcttacca cgtttaaatg tagctgcatc
     3901 tttggattaa gcagtagtaa acgagaatca gcatcatcaa tcaggaatac gccaatcatc
     3961 agttcgttca gtaacgtata taattgctct tctgtggtag tacacgcccg atacatctta
     4021 ctcatttgtt cgctcaacgc attaatcgtc tgatacaatt cttcccattg atcgggtgtt
     4081 tgcatgatta cttccgcctg attaggattt tgcaccattt tttttagtac gggcagcacg
     4141 gttttcaatg gctggttttt ttgatagatc agataataga tcattcctgc aagaacaagg
     4201 aagaaagaca gaaagaagaa aaagacccaa cggcgaaaac tctcagttct cggtaaaaaa
     4261 acgcttgtcg gttctgcgat acgtaaaatg gcttctaact ggccactttt tttgactggt
     4321 aatgcgacat ataaaagatc ctcattcaaa gtagtacttt tgcgcaaagc gctacctagg
     4381 ttcccaccat tcaatacggc tttgatttcg ggacgggagt ttcgctgttc attcaatgtt
     4441 tcattgtttg tgtcaaataa gatctttcca gtagcatcca ttaaagtaat ccgttcgttc
     4501 gactgatgga cgaattcttc caaagcagtt ttgttttgcg ctgcttggag gttttcgaca
     4561 tctaattgcc ggatcagcag ttgtgctttt tttgtcaggt agctttcttg ctgttccacg
     4621 atctgctgtt gaaaataatg ggaaatcaat tgccagccac caaaaaacaa actaactaaa
     4681 agaataagaa agaagagacg ttcataaatt tttttccatt tcatcgtttg ggttcctgaa
     4741 acttatagcc aaatcctcta atagtcacca agtactttgg gtgttttgga tctcgctcga
     4801 ttttttctcg caaatgacta acgtgtacat ctacgattcg gctttgtcct gcgaagtcaa
     4861 aattccaaat gcggtctagc aatgtgtctc ggtcgatcac acggtctttt cgtttcatga
     4921 aataaacaag taattcaaat tctttcggtg ttagttcaat cagatggtcg tctacggtca
     4981 cttgataatt ggtcaaatca gcagtgattt gtccaatcga tagatattca ggctcagctt
     5041 catcttttga ttgcttacga ggttcaattc gtctgaatat tgctttcata cgggctaata
     5101 cttcgcgagg actaaatggt ttggtcaaat agtcgtcagc acctatttct aggccaatga
     5161 tccgatccac ttggtcatct ttagccgtca gcatcaaaat cggtgtttca tttttttctt
     5221 gacgcagccg ctgagtgatc tccatgccat ccatcgttgg aagcatcaca tctaaaatga
     5281 tgaaatcaaa tgattgttct aatgctaatt ctaatccttc gcctccatct gtggcactgg
     5341 ttaccttgta gccttctttt tccaaattga acgtcaagag cgttacaata gaaggttcat
     5401 catcaacaac tagtattttt ttcattcaaa tctcctttaa ataggtttca ttctgtttat
     5461 attttagcat agaatcagta caaagggcta ttttacaaaa gaaataaatg gtataatagg
     5521 aaggcacttt gctgaataga aaggaagagc agtcaatgat tggaatcagt gcatgtttag
     5581 gcggtctggc ctgtcgctat gatggacaga aaaaagaagt accagaattg atggaactag
     5641 ttgaaactgg agaagctgtc atggtctgtc cagaagtgat cggcggatta ccaattccta
     5701 gagatcccgc tgaaatcatt ggtggaaatg gattcgatgt gtggaaagat caagcaaaag
     5761 tatggacgac aagcggcgaa gatgtaacgg aagcgtacaa aaatggcgcg atcaaagcct
     5821 atcaaaaatt gcaggaaaaa cacattgatc tagtcatcat gaaggaaaaa agcccttcat
     5881 gcggaaccca ttcgatttat gacggaagtt tttccggaat aaaaaaaaat ggtgccggag
     5941 tggctactgc ttattttatt cagcaaaaaa tgactgtgct ctctgatgaa gaatggctga
     6001 aaactagagg tgagcagaat ggaaatcgaa aagaccaatc gaatgaacgc gctctttgaa
     6061 ttttattcga ccttattgac agaaaaacaa atgaactaca tggaattata ttatgcagat
     6121 gatttttcat taggtgagat cgcggaagaa tacgaagtaa gccgacaagc agtatatgac
     6181 aatatcaagc ggacaagcaa gatcttggag gattatgaaa aaaaactcca tcttttttct
     6241 aattatattg ttcgagaaca ggtattggaa aatatgactg catatattac agataagtac
     6301 ccggaagata aaaaactact tgaatatgta cagcaaattc aagcaatcga agaataaata
     6361 aaaggaatga agaaaatatg gcatttgaaa gcttaactga acgtctccag caggcgatgg
     6421 gaaaaataag aaaaaaagga aaagtatcag aagctgacgt aaaagaaatg atgcgggaaa
     6481 tccgccttgc cttactagaa gccgatgtta acttgcaagt agtcaaagat tttactaagc
     6541 gcgtacgcga acgtgcggta ggggcagaag tgctggaaag tttatcacct gcacaacaaa
     6601 tcgtaaaaat cgttgatgaa gaattgacga tcacacttgg ttctgaaaca gcagagttaa
     6661 ataaatcacc aaaaatacca actgttatca tgatggctgg tcttcaaggg gcaggtaaaa
     6721 cgacctttac tggtaaatta gccaattatt taaagaaaaa tgaaaatgct cgtccgctcc
     6781 taatcgcagg ggacgtatat cgaccagctg cgatcgacca attgaaagta ttaggtcaac
     6841 agctagacgt tcctgtattt gatatgggaa ctgaagtcag cccggtagag atcgtccgtc
     6901 aagggatgga gttagcaaaa gaaaagaaaa acgactatgt attgatcgat acggcaggac
     6961 gtctgcatat cgatgaaaca ttgatggatg agttgaaaca aatcaaagaa ttgacgcaac
     7021 caaatgagat tttgcttgtt gtcgatgcca tgactggtca agatgccgtc aacgtagctg
     7081 atagcttcaa ccagcaatta ggtattacag gggtcgtcat cactaaactt gatggggata
     7141 ctcgaggcgg ggcagcacta tcgatccgct cagtgacagg cgcaccaatc aaattcatcg
     7201 gttctggtga aaaattgact gacttagaag tattccaccc agatcgtatg gctagccgta
     7261 tccttggtat gggggatatg ctgacattga ttgaaaaagc gcagcaagac tacgacgaga
     7321 aaaaagcgga agaacttgca cagaaaatgc gggaaaattc attcgatttc aatgacttta
     7381 ttgaacagct tgatcaagtg atgggtatgg gcccactgga agatctaatc aaaatgatcc
     7441 caggaatgaa tcaagtccct ggaatcgaaa acgtcaaggt agatccaaaa gatgttgaac
     7501 gcaaaaaagc gatggtttat tctatgacgc cagcagaaag agcaaatcct gatctgttga
     7561 acccaagccg tcgtcgccga attgctgccg gttctggaaa cagtgttgtt gaagtgaatc
     7621 gaatgatcaa acaattcaag gaatcaagaa aaatgatgca gcaaatgtcc aaaggggata
     7681 tgaacatccc aggtatggac caaatgtttg gttcaggcgt caaaggaaaa ctaggcaaaa
     7741 tggcgatgaa tcgaatgatc aagaaaaata agaagaaaaa gaaaaaacgg aaataagatg
     7801 aaaaaaacag cttcggtaca gactgaaaat gtctactgcc ggagcttttc tatcagcaat
     7861 ttttattctt tactaataca attactctaa acgaagaggt ggcacagatg agatgtccgt
     7921 ggagtgaaca aacagaaagt atgcggcaat atcatgatac tgtctggggt gttcctgaat
     7981 atgatgacca aaagctgttc cgaaaattga tgcttgatat taatcaagcc ggattgagct
     8041 ggcagacgat tttaaataaa agtgaagcat tcgatgaagc atatgatcga ttcgatatca
     8101 aaaaagtcgc aaattatgga gaaacaaaaa tcagtgaact aatggaaaac aaaggaatta
     8161 ttcgtaaccg aagaaaaatc gaagcagccg tccaaaacgc caaagcagtc ttgaaaattc
     8221 aagaagaaat cggcagtttt tcagagtatc tttggtcgtt ttctaatgga aaaatcgttg
     8281 attcttctta tcattcacaa gaagaagtcc caacaacaag tcctctttca gatgaattag
     8341 caaaagatct aaagaaaaga ggatttaaat ttatcggaag cacaacaatc tatgcttttt
     8401 tagaagctgt cgggatcatc aatgatcacc ttgacagttg tttccgcaaa gaagaggtcg
     8461 tacgtatcgg cagaaagggc gaataaagca tgcgaaaaat catattttat ggtgctataa
     8521 gtttagatgg ctatttggca acgaaaacgg atgatcttca atggcttttt gacactccaa
     8581 caggtaagca aactacttat gaagcttttt atgaaacaat cgatacgacc atcatgggca
     8641 gaaaaactta tcaggaagca aaaaaatatt tggagaccga agcaatttat ccagagaaaa
     8701 agaatttcgt tttttccacc cacgcacaat tacaattgcc agatgctgag gtcgtaaacg
     8761 aagatcctgt tgcttttcta aaaaatctac aaatgacaga aggaaaagat atctgggttg
     8821 tgggtggcgg tcgattgatc aaaccgctat tggaacatca aatgattgat gaatggtata
     8881 tccagattac ccctgtatta ttaggggacg ggattccttt attccaaaaa ggaacatatg
     8941 aagcgcgttt tgaaatgcta gacacgacac gattcggtga atttatcgaa ttgcattatg
     9001 tacgaaagta gaagtaaata aaataaaaaa ggcaactata tgcagaaatg tcctgtacat
     9061 agttgccttt tttattaccg ttatgctttt tactctgtta atttgttaga aaaatctaga
     9121 tacgcatcta aaatcgtttg gaagaaactt aatgttcctt caacgatatt accattttca
     9181 tcaattagat tcgcaacgtt tccgatatat gcttccggtt gctgcaaggt tggcacattc
     9241 aaaaagacaa gtgattggcg tagatggtgg tttgcgccaa agccgccgat cccacctggt
     9301 gatgcagtaa cgactaaacc tggtttatga tcccaaacac tgtgtccata aggtcttgag
     9361 cctacatcta aagcattttt caatactgct ggcacagaac ggttgtattc aggagtaacg
     9421 aaaacgacac cgtctagtcc cctcacttcc tcacgaaaac gagtccattc agcaggagcc
     9481 tgttctggag tttcgagatc ctcattgtaa aatggcagat catcgatttt tacaaataca
     9541 ctttcgtacc cttcaggaag caatttcgca aaagcttctg ctactttttt gttgtaagaa
     9601 tcttttctta aactaccaat aaaaaatcca attttttttg tcatacgatt tttcctcctt
     9661 caaactatct ataataatct tacaaaagat aagtaactgg agcaaataaa aacacttact
     9721 gacaaaagct acatagaaga tagattactt atttttaaca aaaaagaagt ctaaaatctt
     9781 ctattttcta aaaaaaatcg aatttaatca agctgtaaag aaaaaaagct ttacagggta
     9841 aaaaaaaact gttacactac atcttgtgaa taaaacaatg gaggtggaaa taaataatgg
     9901 cagttaaaat ccgtttaaaa cgtatgggtt ctaaaaagag tccgttttac cgtatcgtcg
     9961 tagctgattc acgttctcct cgtgatggac gtttcatcga aactgtaggt acttacaatc
    10021 ctttgaaaga ccctgcagaa gtagttttaa aagaagactt agttcttgac tggttatcaa
    10081 aaggtgcaca accttctgac actgtacgta acatcctttc aaaagaaggc gttatgcaaa
    10141 aacaccacga agctaaattc tcaaagaaat aaggtgaccg gatatgaaag atttaagcga
    10201 cttagtctta acaatcgttc gtccgctagt cacttatcct gatcaagtca agttagacgt
    10261 cgtagagtcg aaagactttt acgagtacaa tctaacagtg gctcctgaag atattggccg
    10321 tatcattgga aaacaagggc gtgttgctaa agcaatccgc acaatcgttt acggtgttcg
    10381 gatcaatgca ccgaaaaaag tgcgtttgaa tatcatcgat gataaggaaa agtaaataaa
    10441 cgtgctgtac tcgaattttc gagtgcagct tttttgtttt ttttattaat tgaagaaatg
    10501 attatttggc aaaacaagca agaaagcgta tccttatagg aggagggagt gaacagccaa
    10561 tgagaaagaa aatgttattt attccatttg ctctattgtt aagcgcttgt agttcggtta
    10621 ctcaagaatc agacaataca catctatcaa gcagtatatc tgaaactgtt accagtccag
    10681 aaaaaagtag taccaaaacg gcttccacca ctgaacaaac aatggctacc tcaaataatg
    10741 agaaaaagac tgctttagac cagttgaaag aacaacagcc aaatgttccc atgcctctag
    10801 atgtaccagt ctcttcaggc tatctcaata tcgcagcgac acatacaaag caaggttatt
    10861 ctattttata ttacagaaca gatcgtcccc tcgggttgaa tgctgacgaa ttgaatcaag
    10921 aaacgcctat tgcaacctat ctatatcaat atgggtttgc ttctagtcaa gaaacaatcc
    10981 aagttttgca gccatttgag atcgacacaa atggtcagca agtggactta ggttctcgaa
    11041 tcacagggta tcagcaaggt gcagcaggtt caagttttct tgaatggcaa gaaggaaact
    11101 ggcgtattcg aatccgtggg aataatatag aaggccaaga tcccttacta ttagctaaag
    11161 aaatcgtagc ttatttggaa gaaaacagtc taccagcacc tgaacaattt ggtaaaatta
    11221 ctgttgatat gggtgatacg acgaatcgag ccgtagaagt aagctggcaa gaaccgaaaa
    11281 atgcctatac gatcactcat caagatccta tgtctgcgtt gaagatggca gtttctatga
    11341 agaggttgtg aaatcagcgc gctgatctga gcccatcatt taactccaag tcacaggaag
    11401 taatcaaaaa tagcacgccg catttttgca taattttggc ttaatacctt aaaagaaaac
    11461 tagaattgcc tcttcacaca tgctaaaata aaacagacaa aaaagaaatg gggatataag
    11521 ttgactgaat acttgaatgt aggaaaaatc gtaaacacac aaggtatcaa aggcgaagtc
    11581 cgtgttatct caacaacgga tttcccagaa gaacgttaca aaaaaggaac ggtgctaaca
    11641 ctattccaag acggaaaagc acctgtggaa ttaacagtca aaagtcatcg caaacataaa
    11701 aattttgact tattgagttt tgaaggacat ccttctatca atgatgtgga aaaatatcgg
    11761 gatggcatat taaaagtgtc aaaagacaac tctgtagatc tagctgaaaa tgaattctat
    11821 taccatgaga tcatcggtct gcatgtatta gaagatggaa ctgagttagg taaagtaaaa
    11881 gaaatcttgt caccaggtgc caatgatgtt tgggtcgttc aacgtccgaa aaaagctgat
    11941 atattgcttc catatatcgc ttctgtcgtc aaagaagtcg atttagaagc tggagtggtc
    12001 cgtgtagaaa ttccagaagg actgattgac gatgaagatt gatgtattga ctttatttcc
    12061 aagaatgttt gaaggtccta tgggggaatc catcatcggt aaggctgtag ataaaggatt
    12121 attagatatc aatatctcta acttccgcga ttattcagat aacaaacatc agacagtaga
    12181 tgattatcca tacggcggtg gagcaggtat gctgctcaaa gtccagccca tctatgataa
    12241 catccaagcc attgaaaaag ctgcaccgga tgtcaaaaaa agagtaattc ttcttgaccc
    12301 agctggtaaa cgattcgatc agaaaatggc agaagaattt tcacaagaag aacacttagt
    12361 attcatttgt ggtcattatg agggatatga tgaacgtatc cgcacattag tgacagatga
    12421 agtttcctta ggagattatg tccttactgg gggagaatta ggtgcgatgg tcatgatcga
    12481 tgcaacagtt cggcttttgc cagatgtgtt agggaatcag acttcagctc aaacggattc
    12541 ttattcaacc ggtttattgg aacatcccca atatactcgc ccggcagaat ataaagggat
    12601 gaaagtacca gaagtattga caaatggaaa tcataaattg attgaagaat ggcaattaaa
    12661 agaatcacta agaaggacct atcaacgtcg gcctgatctt ttggaaacac ttgaatggac
    12721 acctcaaatg acgaaatttt tagaagaaat caagaaagaa gaacaagaaa aaacagcaga
    12781 tgaagccaag tgactttttc taaaatcttc tttacagtag gttgataata tgttagtatt
    12841 ttatggtgag tgcaaaagca cttaactatt acgatattcc gctgtgagaa aagctcataa
    12901 gaatatttgg aataaggaga agaaaaccat gaatccatta atcgaagaat taacaaaaga
    12961 acaattacgt tctgacatcc cagcgttccg ccctggtgac actgtgcgtg ttcacgcaaa
    13021 agttgttgaa ggtactcgtg aacgtatcca gttatttgaa ggtgttgtta tcaaacgccg
    13081 cggtgctgga atcagcgaaa cttatacagt acgtaaagtt tctaacggag tgggcgttga
    13141 acgtacattc ccattgcata caccacgtgt tgcaaaaatc gaagtagttc gctacggtaa
    13201 agtacgtcgt gcaaaattgt actacctacg tgcattacac ggaaaagctg ctcgtatcaa
    13261 agaaatccgt cgttaatcat tgttatcaga ctgagaaaga acccttggta ttcaagggtt
    13321 cttttttctt agcatgtaat ttgaacaccc tgatccagtt ttttctttaa ttattggtag
    13381 gggagaataa tggattagaa taaatctacc tataagtagt agtaaaaaaa agagcactat
    13441 gataatatct ttcttaagag taaccgcttt aataaaaaat ccagaatatt ctaacataaa
    13501 tggagtattc ttagaatcaa aaggagaaag aagatggttt atacttatac ggagcaagaa
    13561 aagatgaaag ttttagacac attcatgaat gatggaaaag taaaacaagt accaagttca
    13621 acaaaaaaga aatacatcct attgaaagaa gtcttgaaac gctttgacca tggagtgact
    13681 tatacagaaa cagaggttaa ttcgatactg ctgaacgtat tttctagtgg cgattatgtc
    13741 gaacaaagac gttatctaat cacatttggc tttttcaaaa gaagttcaga cggtcgggca
    13801 tatcagatga tgggaattga aaattagaag ttttaattta tactcattga aaaaagttca
    13861 agtatgcgat attggttcta caccgcatac ttgaactttt tttgtcatct gtatctataa
    13921 tacaaagcca agaacgttat tcaaaatatg gatagctatg ataaaagcta atgagcgatt
    13981 cgcagttaaa taagcaatcg tcagtaaaat cgttccagga gcgtacatga aaaaatcaat
    14041 tggatgcgtt acatgaataa agataattct cttttatttg tacgaataaa taccaaaacg
    14101 tacctcctaa aattagattt tggacagatg ccctttaaaa agataacaag atttcgttct
    14161 tgaaacaata tatatgcttt tattccaatg aggttgtgaa aacagtattt tgtcctgagc
    14221 gttagaaaat tgaaaatggg gaatagcaac tcgtacttta ccatttttta agtgaataca
    14281 agatcagcta attgaacacc gtttattcgt gatgagggac acaacactgc agtcgacaca
    14341 tttctctttt atgttcaaag ataacaaata tttaaaacaa agaaaaatag tagataatcc
    14401 cgtcctaact cttttattct tccctcatag cttccaaatt taagcaatac ttcaattaat
    14461 caagttttat cttttttttt caataatcgt aaaaaaagag tattttttat ttaaaaataa
    14521 aatctttgta ttaaaggaca agactaaaat aaccttagaa taaaaagaga aaaa
//
LOCUS       DACEUF010000065        14517 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.068, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000065 DACEUF010000000
VERSION     DACEUF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.068"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(26..319)
                     /locus_tag="IXG20_001953"
     CDS             complement(26..319)
                     /locus_tag="IXG20_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ1610146.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(347..1012)
                     /locus_tag="IXG20_001954"
     CDS             complement(347..1012)
                     /locus_tag="IXG20_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ1610147.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1176..2243)
                     /locus_tag="IXG20_001955"
     CDS             complement(1176..2243)
                     /locus_tag="IXG20_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1610148.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2240..2971)
                     /locus_tag="IXG20_001956"
     CDS             complement(2240..2971)
                     /locus_tag="IXG20_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ1610149.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3139..3618)
                     /gene="greA"
                     /locus_tag="IXG20_001957"
     CDS             complement(3139..3618)
                     /gene="greA"
                     /locus_tag="IXG20_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ1610150.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(3754..4929)
                     /gene="mltG"
                     /locus_tag="IXG20_001958"
     CDS             complement(3754..4929)
                     /gene="mltG"
                     /locus_tag="IXG20_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ1610151.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5094..6254
                     /locus_tag="IXG20_001959"
     CDS             5094..6254
                     /locus_tag="IXG20_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1610152.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(6294..7241)
                     /locus_tag="IXG20_001960"
     CDS             complement(6294..7241)
                     /locus_tag="IXG20_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1610153.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(7374..8765)
                     /locus_tag="IXG20_001961"
     CDS             complement(7374..8765)
                     /locus_tag="IXG20_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAQ1610154.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            8891..9301
                     /gene="tagD"
                     /locus_tag="IXG20_001962"
     CDS             8891..9301
                     /gene="tagD"
                     /locus_tag="IXG20_001962"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAQ1610155.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            9481..10770
                     /locus_tag="IXG20_001963"
                     /pseudo
     CDS             9481..10770
                     /locus_tag="IXG20_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(10837..12264)
                     /locus_tag="IXG20_001964"
     CDS             complement(10837..12264)
                     /locus_tag="IXG20_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1610156.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(12261..13403)
                     /locus_tag="IXG20_001965"
     CDS             complement(12261..13403)
                     /locus_tag="IXG20_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAQ1610157.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIIRVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            complement(13407..14402)
                     /locus_tag="IXG20_001966"
     CDS             complement(13407..14402)
                     /locus_tag="IXG20_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1610158.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
BASE COUNT         4780 a         2731 c         2116 g         4890 t
ORIGIN      
        1 aaaacttgtg tcaccctctc tttttttatt gttttttaaa gtgataggtt ggctcctcca
       61 ggttgggatc ataatcaaca agtaaatcat atcctttaaa gaaccattca tctgcttcct
      121 cgataaaaaa aagtgtccca tccatttcca ctttctttac aggatgttct ggctgttcaa
      181 ctgacattcc aatcgaaaaa ccatcatgaa cctctgtttt gccgtagact tttccaaaaa
      241 aacgtattcc gtaagtttct ggcagatcaa tttctgagtc aaaccacttt tgtgcttttg
      301 gcgttaccgt taattccatc ccaacaacct actttctctt ttctttctat tgattcatct
      361 tatcgtctag aatatccaca tcgcctgtct ctccaatcac cagtagatta tcattttcgc
      421 gaacatattc atctgctgca ggagaagcga taaccgtttg atctactctt cgtatggcga
      481 ctactgttaa gtcaaaacgt tgcctgaaat ttaattcagc taatgttttg ttgaaaaatt
      541 taggattgga gactcggatt tctgctaaag aatatttatc tgacagctct aagtagtcta
      601 gaatattctt tgatactaag ttatgtgcaa tccgtatccc catgtctcgc tcagggtgta
      661 cgacacgatc tgctccgatc ttctctagta cacgggcatg gtactcattt tgggcttttg
      721 ctaaaatatt cggtacgccc atttccttta ccatcagagt aactaagata ctggcttgga
      781 tatcttctcc aatagcgaca attacatgat cgaaatttct tatacctaat gaacgaagtg
      841 tcatctcatc ctgtgcattt gccacaacag catgagtggc tatattcata tattcattga
      901 ctcgatcttc attactgtca atagccagta cttcttgtcc agcttcgatc agtgtacggc
      961 agatgcttcc gccaaatcgt ccaaggccta caattgcaaa attttgtttc accattttcc
     1021 tcctttagta tcttcttttc tcagtataac aaagcctttc caaaaacaaa agcaagcatc
     1081 attattgtaa aaaagccaag aaaaataata gttattttct cagcttttga ttatcctctt
     1141 tatcatcgtt ttaataaaac ttttatcatc tttatctatg cctccttcat caaagggacc
     1201 ttgatctcca cgctcgtacc ttgcccttta aaactaatga tttttactgt tccaccaata
     1261 ccagctactc gttctttgat attgttcaat ccataacttc ccgttttggt ttcattcata
     1321 ttaaatcctg tcccgtcgtc aatgatacgt aataaaagat tgttatctat tttatgcaaa
     1381 tagacttcta attcattagc cttagcatgt cttaatgtgt ttgataacaa ttcttgaacg
     1441 attcggaaca agtgatcctc aatggaacta gttaatttta catcttctac atcccatttt
     1501 agcgaaatct ggattttatt ctgcaattcc ttcaataact gctcaatgcc ttgttttaaa
     1561 cttttttctt ctaaattgac tggacgcaga tgaagaagca gcgcacgcat ctcagactgg
     1621 gaagcgttga tgatctctgc caccattttc aactgcttac ggaacatttc tggtgtctca
     1681 cttttttctg cttgctccgt caatgccgac atcatcatca tcgctgcaaa taattgctgt
     1741 gaaactgaat catgaagctc acgagctaat cgatgccttt ctaattctaa aatctcttct
     1801 ttggtttgtc catctacata ttgaggacgg ctagtcataa tctgtaattc actggatact
     1861 tcgatcattt tttcttttat tttagcaatt tccatatcaa tatctttgat gtacagatca
     1921 tccgacgcgc ttgggatcgg ctgatgcaaa agtttggatt cataattgcc agaagaaagt
     1981 gcacgaagct tttcttcgat tttcccgtat tttgcctttt gaataagtga taacaataga
     2041 aatacgatca atcctacacc taatgaaatt gccatcaaat ggaagataag cggaacgtat
     2101 aataaacgcg cacgcagcaa ttctccccac caatgacttt ggttactggc ataaaaataa
     2161 gtgaaaagtg aaaatagaat aataaggaaa gccgcaatcc ccgaataaac agctagcatt
     2221 gctttggata ttcttcccat catattcgta tcacctcgac atctccaacc aaggtattgg
     2281 taatgatctt caatctgcga ggattttcat cataatcact gctgcagatt ttgatctgtt
     2341 cgttttttaa ggaaaactgc tcttcttcaa acaagacatt tcctactaat gtcgcatgct
     2401 ctaaacgaat agccactccc aggggtacta agatccgtgt tctgcctatc ccttttctta
     2461 caatcacaat attatcatct tttggcaaaa gtgtattacc caaatcgatg atagtatcac
     2521 cagaaatgat ggcaatgttg atatcatccc attcatagac gtcgtttcca atacgttggt
     2581 taccaaataa ttgctgtctt ttacgctcat tattatgtgt ttttaattgc tctgtttgga
     2641 ccatcatgat ctgttttttt cgccaaaaag cattttttgt caaatctatt cctgaaatct
     2701 ctacaccttt cagcccgata aataaaacgg ccaatacaac catcatccat aaagcaggac
     2761 tgttgactaa gctaaaaaaa atgaccaagc tccctaagat caattgaaaa ttttgaaatt
     2821 tagtacgagg atatttgcgc atagctagat atatattgaa aataccgaat attactaaaa
     2881 gcaaaagtcc agtattgttt acgatttgcc aaacagcaaa taacagcagc agtgcttcga
     2941 ctaccacaaa aaaacgccaa gaactattca tcgctttttc tccctcctga cacattctca
     3001 tttcatcata cctatttttt tgactgttgg ctataggact ttagatgtaa gatcgttcag
     3061 tgaaacctgc aatactcaaa gagaaaagat ctagagtgcg tcatcagtaa gatgaactct
     3121 ctagatcttt cagtcagact acgcttgttc tactttgacg attttaacta acatatcgcc
     3181 acctggtgta gcaatggtta cttcatcatt tttacgtttg ccaatcaatg cttgcgcgat
     3241 aggagaatca ttggaaatct ttccagaaaa cgggtctgct tcagcacttc cgacaatcgt
     3301 gtactcttct tcatctccat caggcaactc gatgaacgta actgttttac cgatcgatac
     3361 ctcatctgca tctacgccgt cattatcgat gatttgtgca aaacggatca tattttcaat
     3421 tgttgtgatt cttccttcaa caaatgcttg ttcatcttta gctgattcgt attcggagtt
     3481 ttcggaaaga tctccgaaac cgcgagcgat tttgattcgt tcaatgattt ctccgcgttt
     3541 aactgttttt agttcttcta attctttttc taatttttct ttaccttcta gagtcattgg
     3601 ataaactttc tctaccatgt gtaacgtctc cttttttcga taaaataccg caaaaggtaa
     3661 tcttttgagg aatatagtcc aaaaaagatt accacgcttt tcttaaaatg taaatgtttt
     3721 tctaaaaatg atcaatgact agcccctgtt tttctaagaa ttatttacat atttttgcac
     3781 tagttccata tgttcttcat acgttttagc gaaataaaca tttccagtgc tcgtatctgc
     3841 tacgaaataa taatagtcat tttctgctgg ttctaatact gccttgattg ccttttcgct
     3901 tggattgtca aaaggaccag gcccatagcc ggtattggta tacaaattgt atggtgaatc
     3961 tacagctgta tcttcatacg tcaccaattc tttatgttct cctaatgcat ataaaacaga
     4021 gatatctgac tgaagtggca tatttgcctt gattcggtta aagaagactt gtgcaatatt
     4081 tttacgatca ttttctttta cgccttcttt ttcaactaga gaagccaaag ttaaaacttc
     4141 ttgtacagtc aaatttttct gtttgatagc tgaataatag ttttgcatga ccgtatttgt
     4201 cttgttcacc atctcaatca caagatcctt aagcgaagta ctcgtgtagt aatcataagt
     4261 agctgggaat aagtagcctt ccaaacggta ttttgtgttc tcagctttag aggcactttc
     4321 caacaattct gggaattttt gatacaactc gttaaagaaa gtctcatcat ttactaaatc
     4381 taaaaattct ttcttgtctt tacctgttac cttcgctacc cgctcagcaa tctgatcgat
     4441 gtcgtagcct tctggaatgg caattttccc atctgcaact tttgttggct cagatgtgcc
     4501 gccttcttgc aactgcttgc caatctcatc aagagtcata ttaggcgcta actgataata
     4561 accagcttga aagccagtta agtttttaaa ttttgtataa taattgaaaa caatcccact
     4621 tttaataata ttgtctttct ccaaaatttc gccaatctgc ttattagaag agccgtttgg
     4681 gatttccact tggactaatt ggtcgtccga tttatctaaa ggcttcaacc cgctatctac
     4741 ataacgatat acggtaaacc cgagaaatcc gccaataatc aataatgtaa gagcgatcac
     4801 taaaatgatc ttcctcacga tcttatcttc ttttttacgc agatttctcc gcttgtcgat
     4861 tatatctgct gtagctgcct tactggcttc ttttttgtct ttgcttgggt gttgtttgtt
     4921 tcctgacaaa acaactcctc cttactatca tcttcgtaac tctgtttatt atacatgaaa
     4981 tttctactgc cgtgaattat tttaccaaat attatgtcaa aattgtaagt ggcaaatgct
     5041 ctttttgagg attacaatga aaaatagtta aaaatggata gaaaggattt aatatggcaa
     5101 aaggaaaaca tcaaaaaaaa cagcagatct ctctttcttt tctcgcagtt ttgttgctgg
     5161 ctgtcagctt cttttcaaca ggcatcatta caggatggca attaacaaaa caacctcaac
     5221 cacaaattta taaaacggaa cccgcacaac ttgcttccaa tttaacatct aatgagcaga
     5281 tgcttgccag caaaattgat caattactgc aaaaatcaga ttatatcggt tcgatttatg
     5341 tacgcaaaaa taatcgagtg atattagaaa aaggatatgg gtacgcaaat ctacgcataa
     5401 aagaatcgaa ttctccttct ctttactatc aaatcggttc gatccaaaaa gcgatgactg
     5461 ctttacttat cctaaaacaa attcaatctg gcaagataac ttttgacaca aagctatctg
     5521 aattttatcc tcagattcct ggaagtcagc aaatctcaat caaaaatatg ctttacatgc
     5581 gctctggtct tcatcgaacc gcttctccaa aaaaacctat gtcagatagt gagatcattc
     5641 agtttgccct tcatcatttg aaatttactg gttatgatac gtatcattat gaaccattaa
     5701 attatacatt gcttacaggg gtattgatca aactaatgaa tcagccttat gaaactcttt
     5761 taaaaaaaga aatcattcgg ccactccatc tagaacatac agatttttat gaaaatgtaa
     5821 aaaattcccc tcagcatgct gtatcttacc aaatgtctgc aactgacgat tattcaaaag
     5881 cattggtcga gcctgaaaca gacatccgaa atgaattagg cacaggaaat ataagtatgt
     5941 ctgtttatga cttgaacaaa ttctttacca gtgttctgtc tggcgatatt ataccaaaag
     6001 aactactttt ctcattgtgg caaaataatg gtgataaaca tccttacagc ggcggtgttt
     6061 acagtggaaa tgattatatc cttgctcagg gaaatatcaa tcgattccat gcagtagcgg
     6121 cgatgaaaaa agacacaaaa gatgcaatcg tcatggaaag taatatccaa tctgataaaa
     6181 acatcaaatt gccggccact gatttaagaa atcagattta cgaactgatg gaaggtgtta
     6241 atttaaaaag ctaagtcgag acaaaaaaac cagcaggatg ctggtttttt gtttcaatcg
     6301 atttctgtct ttaaataacg ttccattccg ttcaatatat caaatttggg ttgataacct
     6361 aaagaacgga tctttgaaat atccgctaaa gaatcacgaa tatcccctga acgctccgct
     6421 tcatatttaa gcgctagctc tgttcctaag atttgatcaa ttgaatgaat caattcaagt
     6481 agcgtagtgc tttttcctgt accgacattg aattgctgtc ctaaagcttt ttcttcctta
     6541 gcaacaagta aaagtgcttg tataacatct tctatataaa cgaagtctct agattggcta
     6601 ccatcaccaa ataaagtaaa ttctgttttt tctcctgcca attgtttttt gtatcggtcc
     6661 actagaatcg aaataactcc gctatatgga gagttaggat tttgatttgg accataaacg
     6721 ttaaaaaatc ggacagcact agtcggaaca tcatataaat gacagtaatc caaaacatat
     6781 tgttctgatg caaacttatc gatggcatat ggtgttaaag gacggatgac cgattcttct
     6841 ttttttggca acgttggttc atcgccataa actgcagctg atgaactaaa taccaatcgt
     6901 tttaaaccag attgatattt tcgtaccaat tcaagcaata aaagcgcact gtcaaaattt
     6961 acacgatgag tttctagagg acgttctaca gaatctgcca cactagctac agcagctaaa
     7021 tgaaaaatat aggcaaactg atactgtttc atgattcttt ccatcaattg aggatctgat
     7081 acatccccct caatgaaagt cacttgtttc tccatatcca agttttcttt ttttcccatt
     7141 gataaatcgt cgactactac gattttgttt tcttttccca aataattcgc tagcgttgaa
     7201 ccgataaaac cagctccacc agtaatcaat atagtatcca tcaaattcac tccaattatg
     7261 cattctcata cttaataaag ttagtttacc atttttatat tgtaaaaact ataaaatttg
     7321 ttgaagttgg gataactagt tttcttttct actttatata cctaaaaata gtttcaatta
     7381 gttactcctt cttctttaga tatagttttt tggatagaac ccagatatcc ccaaaaaata
     7441 aatgaaccta tcgtactaac caaaatgagt tctgtgataa ataatgccga aactgctatt
     7501 gtaagaatca ttaaaatagc aaaaaagtgc ttatctttta ctagatttgg ttgtgtgaaa
     7561 caataacgca aagttttaaa gcattggata aataagaagg ttaacaatgt caatagacct
     7621 aatcctcctg tagcagccat aactaagaaa ataaaattat gagcagttaa agtatctttt
     7681 cccattaatg tatatggtag tttatcatgt gcgtattcaa tatagtatcc tggagaaccg
     7741 ccggatatcg gcttactctt gaatatttca attgcacttt tccaaagtcc aaaccttaaa
     7801 ttagaaacat ccccgccatc agtgtcaggg cgttttaaat caactttatt gctgctagtt
     7861 gttcctgatg aatcagatat atcttttttt atttctgtaa tatgcggaac atgtgcccat
     7921 gcttgagcag ttatatctgt tacataatac atcgcaatta tacttagcaa tcctactact
     7981 aatgcagtta gccatctttt taaagtatta gttttaagaa aacgactact ataacttgta
     8041 aagaaactaa aaacaaaagt tgctattaat aaacaaacta atgtggtgcg tgagcccgat
     8101 aaagcaataa acgagaattg aataaaaata ttaattgcaa aaaatatttt aaatagaaga
     8161 tttaaatgct tgtaagtgac caaataatat atagcaataa tgatgacaat taatgatata
     8221 accgcaccaa aatttggatc tgtatataca ccaaataaac ggttgcctaa ccaccctaaa
     8281 cgaactgggt aatacttata ttttattgga atttttatgc catattgaat gacaaaccag
     8341 tataatgata ctaaattagc taaaaaccat accaatgaaa gtatccaagt aaatactttg
     8401 aaaaaaaagt tactactgta gtctttgcca aactgataaa tcataaagaa aaagattaat
     8461 tgccacatga ttaacttgat attttcgcta tatccatatt ttaaattcat taaactgctt
     8521 actagcatcg cgccaataaa agcaattaat agccaatcat attttttaag atttttttct
     8581 ctaaatgcaa taattgcatc tataatgatc aagacaactc ccgctattcc agcaggccaa
     8641 aaaagtaaag tatcatatct agtaggtaat agtaaatacg ggcctattgc tcgtaacatt
     8701 agaataaaac caaaataaat aaaataccaa cctttcacat attttaaaaa tttttcattc
     8761 aacatttttc ctccatccac ctatttctca taaacatttc ttatcctttt taggataaac
     8821 tggttctttc aattctaaca aaaaaatgct aaactaacaa tgtatattaa tgaatttcga
     8881 gaggtgaaag atgaaaagag taattactta cggtaccttt gatttgcttc actacgggca
     8941 tatcaattta ctgaaacgag caaaacaata tggagattat cttattgttg ccctttctac
     9001 agatgaattc aattggaatg cgaaacaaaa aaaatgttat tttagttatg aaaaaagaaa
     9061 acagttactt gaagctattc gctacgttga tttagttatt cctgaagaaa attgggaaca
     9121 aaaagtaaaa gatataaaag aataccatgc agattatttc gtgatgggcg atgattgggc
     9181 tggacaattt gatgaacttg aacaactaac agatgcacaa gtaatttatt taccaagaac
     9241 acctgagatc tctacaactc aaataaagaa cgaacttaat tctttaaata gtgataaata
     9301 atatttttta aacaatgaaa gaacaatata tctggataag acgtaaaaaa atggctctat
     9361 aatttctagg actgatagaa ttttctgtcg atcctagatt ttcttgtaaa aaaaacagac
     9421 atacaaaaat ttcgataaaa tagagtcgtc taaaacatca atctgaaagg aagattttgt
     9481 atatccattt ctcgttctat caaagagcac ttcactatta aagataaaaa tattgaaatt
     9541 aattcttttc aaaaaacttt ttataaggac aagaaagcca tcgtaatcga aggtacatta
     9601 gttcgcactt ttagacattg tctattttgt aaagaaactg cgaagcagct cgtgaaaaac
     9661 ggcaagaaga tttcaatgat tatcttaaat cgcagtggga ataaataaac ctatttgcgt
     9721 cttagaaaac aacattacca ctaccaatca tgcggaaaat attttactgc tcatacgtat
     9781 cttgtcgttc cttcatgttt tatttcgaaa caagttcatt acacgatatt agaagagttg
     9841 accgagcgtc aagcgttaaa gacacttacg aaacgttgtg atctttcagt aacgacattt
     9901 caacggacat tatctgctct atcggcaatg ctgcgacaga tgctagacta tttacctcat
     9961 tgtttactat tcgacgagtt tcgctctctt tcaatttccc atggaaagtt ctccttttct
    10021 tgtatgaata gagagacagg gaagctcttt gacattttgt ccagccggag aaaaaaagat
    10081 ttgattgctt atttcatgcg ttttacaaga aaagctcata tgggggttcg atacattgtc
    10141 acaaatatga atgcccctta tttttcatta gtcaaagagt cctttccaaa cgcccaaatc
    10201 atcattgatt gctttcacat tggacaacac ttgaacaagt ttttcgatcg tgttcgaaaa
    10261 cgtgtgatga agcagttgaa tcaaaaagac acttcacaag ctaagtacta tcgacaattg
    10321 aaacctttgt ataaattact tctaaaatca gatgacgagt tggattacac aacattcaag
    10381 aaatggcaga attttcaatg gcgctattta acagaaagtg aagtagttga tcgtctaccc
    10441 tcaatctctt atgaacttaa aattgctaag cagtttatca aaagctgctt agcaattatc
    10501 acaagaaaca gctagctact tcgagaaacg accaaagaag ttccctcgaa acttcactat
    10561 ctcaaaaagc ctttttcata tctgaaaaag gcttttaact agcattttcg aaaaagtatt
    10621 ctaataacaa aattagagaa tcttcgcaca catatcaaaa cattgaaacg atttgcttat
    10681 gggtttcgca gttttaccaa tatgcgcttg tggatttttc taatcaacag gttaatcagc
    10741 tatgcattaa aagctagtaa ctcttactaa ctttgttgtt cataaatgac cttatcagcc
    10801 cgatttgaca aagaactact tttttacaac attattttat tttcgagtaa tctttcttat
    10861 ttcatttcgt atatctggaa aaagaaaata aacaaacgga agataaataa tgaaaatcat
    10921 tattgattta attgccatat ctaaaaatga agaagagaat aattgtggaa gaaagtaata
    10981 ggcccaagta gtcaaagtaa gattgactat aaaaaataag ataacgttct tcccaaagaa
    11041 ataggatatt tttatttctt ttcgtacaaa aaatactcta aagaaaaaga ctaataattc
    11101 tactaccacg agtacgatcg tagccgctac agcgccataa gtaggcacaa aagctacatt
    11161 tccaataata cttacaatcg caccaatgat cattggataa gcatactcct tatctttttc
    11221 tatagcaatt gcaaattggt tagcaaaaac tccaccaatt ggactaacaa ctataattag
    11281 acaagaaata agcatgttta ttgtcatcgg tttgaaattc tccccaaaaa accatggaac
    11341 aaattctttt gtattgataa acaccacact aaaaagaatt aatgcaatga taatcgtgta
    11401 atcaaaagat ttttttgtgt aaactaaaat attttctgat ttcccctctg cctgtgcttt
    11461 agatatttta ggcaacataa caatagtaat tgatgttaag actgccaata taatacgagc
    11521 aattttttga gactgatcat aataagataa ttccgttgga tccacaaaat acccaactat
    11581 cgttttgtct aaagttgtgt acacttgcat aaacaactga ggagtcaaaa taataaaagc
    11641 tggtttccac aactgtttaa aaacattaga gttaaagatt ggtttaccta cttttccgtg
    11701 gatacttaac cagaaaacta aacttcctaa aaagttagta attgaagtaa tgaacacgta
    11761 aatccacaaa tcatctttag ttgaaacaaa aatgattatt aacaataccg atgataattt
    11821 gataactgta tttctaataa ccactttcga aaattcttca attccttgaa aaaaccaaga
    11881 aatatctaat gttagcccta caataaatat caattgaatt aagtataaat ttttatactt
    11941 tacatcaaaa aaataaatat aggctatata gagaaaggaa acactcaagc ctattaaaaa
    12001 ttgaataaag taaatattcc agaattgtaa agatagtgct tttttatctt tccttgattt
    12061 agcgatattt cgaatacgaa aaggattcat tcccacagca atgacagtca ttaaaaactg
    12121 tgatattgag aaaacatacc cataaatccc taaaccagtt gaaccaattc tacgactaag
    12181 tataggtata gtaagtaagg gtaaaacaat caaaaagact tgatatatcg cattataaaa
    12241 tatatctttt actgtttttt tcactttata tccctctcta tcaactcaag aaccttttct
    12301 gtacttgctc ctgtttcata atttccaata taatcaagta actcttgaga acttttcaaa
    12361 agcttttctt gttggttttt aaaaacatca acaaggccat aactatcttg tactcttata
    12421 attggtaact cacttaactg tatataaaaa cctctttcta agctatattc tttttcatca
    12481 tagttaaaaa gaataattgg tttttttgtt agcgcaaaat cgaaaaaaat agatgaataa
    12541 tctgtgatta aaacatctat ctcttcaaaa agcatttcaa gaggaatccc atcattaatt
    12601 aaagtaattt gatgtttttt catatattct atacttttag aatcagttgc taatttacta
    12661 tgaacattag gatgaaattt agtcataaaa tttaattctt gttgttgctt tttaaaaaat
    12721 tcacgtaagt tatccacacc taaccattct agttgcgtaa ttccgttgtc ctcaatatta
    12781 tttcgaaaag ttggagcaaa taagacattg atttgtttct ttttcaagtc aaacacttgc
    12841 tctttactag aggctaattc atcgtttcta ggagttccta cttctaatat tttagaagga
    12901 acgatattga atgctgaaac atacttctta gaactatagc gattaccaga taataaatat
    12961 gtaattgact cactatcttt tttcgcatag cttaggtaac gtttaggtaa cttatctttt
    13021 gcatcaaatt caattttttt taaaggcgtt ccgtgccatg tttgaaaata aagttgtttt
    13081 ttcctttttg catacttaat cgttttccga ctatcgtcta tccaatactt agctgtatat
    13141 aaataaaagg cagctaaaaa agaatcggag tcaattcttt taatataatc tggtaaatca
    13201 gttataatcg cttctttttt gattaaccaa ataattctgt aattcaattt tttttcatga
    13261 attttctctg aaataactct tggatttcca gaatagttac gcccgccaaa acttgaaaat
    13321 acaattaaat ttttttgaat aggtaatatt ttaataaaaa ccgggaaaat attccttgaa
    13381 aaaaattttt ttatattctt catctcttac ctcacagatt ctatcttaat caagaattta
    13441 cttgctatct tactacttga catatagtaa ggcaagtttt tatttattaa taaaaacttt
    13501 actaattttt ttaattttgg tgaaaagtcg ggatcaattt tcacttcttt ttcaaagcgt
    13561 tgtaaaatat ttatagaacg taaataatct tctttattgg tatagttatt tttaatcatt
    13621 gtcatcatca tccttattgc ttcatagaca tagcgagttc taacattaat tgataattcc
    13681 ggataaaact tatctatgaa tattagtata tcttcatgga cttcaaaaga atcaaaaatt
    13741 ctaggattga agcttcctct cagtatactt ttagggcttt ggtaatagtg atatttagca
    13801 gatgaattga cagcaacttt ttcggaatga tcgatcagca aataagtagt agccttatct
    13861 tccattaatt tacccagtgg gaatcttata ttatcaaata aatttctatt gaataattta
    13921 ttatttgcag aatagccaat tttattattt ataaataagt ttttcattgc ttgttctcta
    13981 tcaaatattt cccattttct ctcagcaatt gtatgttctt ttagttgttc taaacttgaa
    14041 atctttttta tttttagcac cgagatatct gctccataag tttgcgtgcc aaaatatagt
    14101 aactcaagat agtctggttc tatccaatca tcgctgtcaa taaagcacac aaaatctcct
    14161 gttgcagaat caagaccaga attcctagcc gcacttaaac caccattcac ttgatgtatc
    14221 acttttaatc gcatatcttt ctttttatat tcttctgcaa tttccccact tcgatccggg
    14281 gaaccatcat tgactactat tatttcaata tttgaatgtg tctggttgga aatagattct
    14341 aagcagtttt ctaaatagtc ttctacgtta tatgcgggta ctataacact aattttatgc
    14401 actctcttca cttcctaaaa atcatctttt ttatttttac aaatgtatca ttaccagtaa
    14461 caattttagt cttccgaagt taataaacat aaaaaataac atatcgaaaa aaacttt
//
LOCUS       DACEUF010000066        14363 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.069, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000066 DACEUF010000000
VERSION     DACEUF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.069"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..135
                     /locus_tag="IXG20_001967"
     CDS             <1..135
                     /locus_tag="IXG20_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1610159.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            113..559
                     /locus_tag="IXG20_001968"
     CDS             113..559
                     /locus_tag="IXG20_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610160.1"
                     /translation="MESFVSLNLTNNIILSGCSSNKKSEAKEDNSLLQPGTVWKEEVG
                     GLVNNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGIDLFIGKTA
                     LIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            556..798
                     /locus_tag="IXG20_001969"
     CDS             556..798
                     /locus_tag="IXG20_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610161.1"
                     /translation="MIASLFIYPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            816..1118
                     /locus_tag="IXG20_001970"
     CDS             816..1118
                     /locus_tag="IXG20_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610162.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            1188..3242
                     /locus_tag="IXG20_001971"
     CDS             1188..3242
                     /locus_tag="IXG20_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610163.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLALHDTEGKIEKTGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWTKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYLGLRTDKLKISKELNEQLNKLYQTT
                     TKVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRKKEKEHEQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFDGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESNMENSKESDSSKTKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            3242..5851
                     /locus_tag="IXG20_001972"
     CDS             3242..5851
                     /locus_tag="IXG20_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1610164.1"
                     /translation="MRIRETTKRKDKPFNLEKKKYLSHFYSYAPAGERLPGKHDKRVK
                     QKLMYSGLVYLPLDYLVTRIPEAFSRFEIIQGSLQGVNLFQLQEMPQVFHFDSAGFWN
                     PLTPATSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPDAKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKNELYVASKDELEARGYDVYAYNLTEPLQSMSDNPLALIVKYWQRGDV
                     DTATQLANTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPTNEFKQLNALDEFMNQLPPGNLAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDQVRLKYDHQRKEYVAWYEFSQRELRDEEGNIV
                     YRKKRGETHLPEWEKEAVLTLDESKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     TNSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLHQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            5848..8244
                     /locus_tag="IXG20_001973"
     CDS             5848..8244
                     /locus_tag="IXG20_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610165.1"
                     /translation="MIIGGIFSGIANWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLETDPNKVESLPTDKRNALTAKNFKTANINSVII
                     PKEAAKMADEDKSGLSDIQKDRLRALQYRLELDDNGKEIPVEISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSNLNDIRKNGF
                     GNRKDVIPDNGEEQPDDKATIVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQY
                     RYKSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSMKIGLPKTQNLP
                     MKDSRLNQFKENDQSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNVSIQN
                     VVTREAAPEPIQKVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLRDIKQDIKP
                     "
     gene            8296..8628
                     /locus_tag="IXG20_001974"
     CDS             8296..8628
                     /locus_tag="IXG20_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610166.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            8629..9222
                     /locus_tag="IXG20_001975"
     CDS             8629..9222
                     /locus_tag="IXG20_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610167.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVQTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            9385..11409
                     /locus_tag="IXG20_001976"
     CDS             9385..11409
                     /locus_tag="IXG20_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1610168.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASRLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            11409..12614
                     /locus_tag="IXG20_001977"
     CDS             11409..12614
                     /locus_tag="IXG20_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1610169.1"
                     /translation="MENQDESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNDNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            12630..13274
                     /locus_tag="IXG20_001978"
     CDS             12630..13274
                     /locus_tag="IXG20_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610170.1"
                     /translation="MENKTLKIVLTAALSISLIGNIALFWQLKEEQATNTYKIEEVAK
                     NASEKLKKNFSEAQAKIDKLNQTINEKNQEIDQLKQQDGIKAKEEVTDAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAETS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            13285..14091
                     /locus_tag="IXG20_001979"
     CDS             13285..14091
                     /locus_tag="IXG20_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610171.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFTILGFLILASPIYTHKKVNI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQGLLKELANLDYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIVPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDDNYEQHAINYKVKGYKKEQKAAQKQIESLQATIDLNEELVNKLKDQLP
                     YQVADDQENTENKINDYQQEIETSKTQMKDQEEIIQKIQEKIDRMTKENI"
     gene            14114..14344
                     /locus_tag="IXG20_001980"
     CDS             14114..14344
                     /locus_tag="IXG20_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610172.1"
                     /translation="MKGANLVGKDIFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKFRSFEALYLAAKKYFTAERK"
BASE COUNT         5275 a         2244 c         2990 g         3854 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tattttaagt ggatgttcaa gtaataagaa
      181 atcggaagct aaagaagata atagtttatt gcaaccagga actgtttgga aagaagaagt
      241 aggtggtctt gttaataatt tgaaaattat agacgataca acatgggaat attccgaaag
      301 tgtttggcat cctgaacaag tacaaataac agttgagaag ctaaaagact ataaaggatt
      361 agaaagatat aaggtaatcg attcaggtgg aatagattta ttcataggta aaacagcctt
      421 aatagttccc tataacaaag acggtttaga aacgtttaaa tttattccaa caaacgaaaa
      481 agaacatcga gataaaagtg aaatattaaa agaatacagt actaaatctg gatacaagtt
      541 acaaaagact agcgagtgat tgctagtctt tttatttatc ctgaaggagg aatcacagtg
      601 gcaaaagcta aaacgccatt tgaaaaagcc gcgtctaaaa tcttagaacg ccgcggtgta
      661 tctgaaacac agtggaaaca agaagtcctt gaaaaagcac aatttgacct attacgggga
      721 aaagacaagg acttagaagg atatatctat caacgtgaac gtgaaaagct tgttttcgat
      781 gaaattatta aacactaaaa ggagcgaaca attttatgtt tgaatcaatt acaatgaatt
      841 ttttaacaca accgaaagta gtagctgcgg ttgatatttt tagagcatta caaactgcag
      901 caactagttt aaaaaacaat ttgatgattt ttgctgctgc tgcggccgtt gcttccattg
      961 ttattggggg tcttttcttc ctatttggac gaagaggagc tgaaaatggg aagatgcacg
     1021 taggctatgt attgatgggg gtaggagtta ttttagcagc agcttctatt gtggtttcgt
     1081 tatttggatt gtttggacaa acacctcaag gattataaga aagtaaaaaa gtaggagtaa
     1141 gaagtagtcg atacttttta ctcctttttt atgaaaggag aatactcatg tactttacga
     1201 ttgcaggcaa taccaaaaca gataaagagt taataaaaaa agcaacgaag gaagtaggaa
     1261 agcgtacttc ttttttaccg aaaacaatta aatattctgt cattgaaacg gaagaacagc
     1321 tacatgtgtt agggaaagta ctggaacacg aaagtcgatc ttctgttaag ttgactctcg
     1381 cgttacatga cacagaagga aaaatagaga aaactggtac agaatacgtt tttggcccac
     1441 tggattatca aatttcagaa ggtttctatc ctacattgat ggatttaatc cgccttgatg
     1501 tgtttaaaga aacggatgaa actatgagtg tggaagagtc ttatcgaaac gaaaaacttt
     1561 tacaagtttt agaagaagag ttagagagcg accaaagaaa agtgaatcaa gggagtgaag
     1621 tcgaagtaaa tgaagaagta ccatgggaac gaccaatatt tccaaagaag gagtcaaaag
     1681 aaaaagagac agtctatgtt gacgaacaag ctgatattcc gactcagaaa tcgatatttg
     1741 aggaagagaa cgatgtgatg ggtcaattgg aggtagatcc ctttgaagaa acaatggagg
     1801 atgaagagac ggccgaagta gaagttgatc cagttgaagc taatgaagag tcaacatgga
     1861 ctgaagggga ttctcaagaa tatattgaac cacttatcag tgaagaagta gaaaaaaacg
     1921 aaatagagat acaaaaggca actgtatttg attatatttt ggatcaatat ccgccagagc
     1981 atgaatggac gaaagaaaaa aacaaattat ttttaaatga gctttattcg aaatatcaat
     2041 taccccaaac ctatcaagca tttttagaga gtaaagaaga attaatctca cagggtaaag
     2101 gcacactttt agaacagtta gagtctgtcc aatcaacaaa ttggcatgag cttgcacaag
     2161 aagatttatt agccaccttt gaagaacgtc aacaagaaac agatcaagaa attaatgagt
     2221 atgcgacgga acagtcgtct aattgggaga aagcaaaaga ggaactaaat aaagaagaac
     2281 agcgagtgat tgaagaagaa gtacaaaaaa ttaaagagcg atacgaaaag aaacgcgaca
     2341 atgcgtattc cttgtgtgta gaaaagatca ataattttac tcagacgaat caagaagcgt
     2401 tatcgaaaga aaaagaaaac ttgttaaaag aacgtattaa agaacgaaaa gaagaatttt
     2461 acctaggttt gcgtacagat aagctaaaga ttagcaaaga attaaatgaa caactgaata
     2521 agctatatca aacaacgact aaagttctta tggaaaaaca cgaacagatc cagcaagaat
     2581 tacaagtaca aattccaaaa tggaaaaaag agtatgaaat tcaacaacaa gaattgaaag
     2641 aacaagaaga aaaacaacgg aaaaaagaaa aagagcatga acaattagaa ataagaaaac
     2701 gggaacagga tcttgcggaa caaaaaatgg cgatagaaaa acaacggtta gagcaagaaa
     2761 aggaaaaaga agcagctgct cgtaaggaac gtcaagaaca aatggataca ttgcgtcaag
     2821 cacagttgaa ttatctagca caacctcaac cagtagctgc tgttcaaacg ccagtgaaca
     2881 accaagcaaa tttaactcct tcaccacagc gttcctttga cggatggatg gtgggatgta
     2941 ttgcaagttt aagcttgctt ctaggtggag ggacagtatt tgcttttaat catatcaatc
     3001 aatcttcaca ggctgattct gtagccacaa cggcgtcaat caaggaagaa gcccaggaac
     3061 aagcaagaaa agagtatgaa caacaattag aagaattgtt gaatcgtacc aatcagtcaa
     3121 caacagagtc aaatatggaa aacagtaaag agagtgattc tagtaagacc aaagaaggat
     3181 caagcgattc aactgtacaa tcaaacactt ccgaaagttc agaatcaaat gaggtgaaat
     3241 aatgcggatt cgagaaacaa cgaaacgaaa agacaagcct tttaatttag aaaaaaagaa
     3301 atatctatca catttttata gctatgcacc agcaggtgaa agattaccag gtaagcatga
     3361 taagcgagtc aaacaaaaac taatgtatag cggtttagtc tatctccctt tagattattt
     3421 agtgacaaga atacctgaag ctttcagtcg atttgaaatc attcagggaa gcttacaagg
     3481 agtgaatctg tttcaattac aggaaatgcc acaggtcttt cattttgatt cagctggttt
     3541 ttggaatcct ttgaccccag caacgagttt aggtacacgc ttagttgttt ctacgatttg
     3601 tgttgttcca gctgtactta tgggtttgaa aatggacatg tggtttcgtc cgctagcgga
     3661 tggacaaaaa ggggataatc ggttaatgac actaaaagag attcaacaaa tctatcctca
     3721 aataccagac gcgaaaggtt ctttccctgg atatggtgga attcctatca ctcactataa
     3781 aaaatattgg tatatccaaa cagataccgt taatacttgt attgttggta cttctcgatc
     3841 agggaaagga cagattgaag tattagcaac tattgataat ttgtctcgtg cagaaaaaca
     3901 aagttcaatg gttgtcaatg atcctaaaaa tgagttgtat gtagccagca aagacgaatt
     3961 agaagccaga ggctatgatg tttatgctta taatctaact gaaccgttac agtcaatgag
     4021 tgataatcct ttggctttaa ttgtgaaata ttggcaacga ggagacgtgg ataccgctac
     4081 gcaactagca aatacattta caaatacgat ttattatgat gccaatgctt cagataacaa
     4141 atactttaac gaaaacgcgc agaaagctgt caatgcgctt atttttgcgt tgttagaaca
     4201 agccgatcaa acaggggatt actccaaatt aacacccaat aatcttgttg agcttctttc
     4261 tgaaattggc ggttttaatt atcaagatcc tactaatgaa tttaagcaac tcaatgcttt
     4321 agatgaattt atgaatcagt taccacctgg aaatctagcg aaaaaacaat atggggcaac
     4381 aaaaattggt tctgataaag caaaaggcaa tattctatca acagcgatca caggattgaa
     4441 tccatttacc ctaacaaaaa ttgcgaaaat gacttctcaa tcaaccattg attataaatc
     4501 ggttggtttt cccaaatatt tggatttgaa attacatgaa agtcttttaa atcaacgaat
     4561 cgtagtggaa ttttatcatc aaggaaagaa atttcatgaa gaacgagtaa aagttggtta
     4621 tagaggtttt tgtgaaatca actttgactg tcatttagag caaggggatc aagttcgttt
     4681 aaagtatgat caccaaagaa aagaatatgt tgcttggtat gaattttctc aaagagaatt
     4741 gagggacgaa gagggtaata tcgtttatcg aaaaaaaagg ggagaaaccc atttgccaga
     4801 atgggaaaaa gaagcggttc tcacactaga tgaaagtaaa agtaacttat tagtagagtc
     4861 tattcggatg cactataccg ataaaccaat ggctattttt atgctcacac ctgattatga
     4921 tccatctaac catatcattg ttagtatttt tcttagtcag ttgtataaag aattatctac
     4981 gcaatgtgtt aagactaaag gagataaatg tcaccgtagg attcatttta ttttagatga
     5041 atttgggaat atgccaccaa tggataatat ggaaggaatc atgacagtca cagctggtag
     5101 aaacatgttg tgggatctgt ttattcaatc ctatcagcaa ctttatgcga agtatgggga
     5161 acaaaacgca gcgattatca aagaaaattg tcagacacat atttatatta tgtcgacaga
     5221 cgataatacc atcgaagaat tttccaaaaa agtggggaat aagacggttg aacaagagaa
     5281 cagtaccacc aattcattag gtatgaatac tcatatcaat cgcaatgttg attcagatcg
     5341 tatcttaact cctgaaagaa tttctacttt tttagaaggt gaaacgattg tactcgctcc
     5401 tttaaaacga caagatttga aaggtcataa aatacggccg tatcctattt ttaatcatgg
     5461 ggaaacgaat cttccttatg cttatcaata tattgaagat ttcaatccat cgtgggatat
     5521 caatgatata gacatacatt gtacacatgc ggacttggat cttaaggctt tacaaattga
     5581 ttacgattat tttctacctg atatctatgc acgtcgactt tatcacaata tgcacgatca
     5641 aatagaagag gaagctaaag aaaacatttt tattaaaatc gttcaatctt tagacaactc
     5701 aacggcacaa aagttactta ttcaatttta tgaagagaag caaatagatc gtttgcaaga
     5761 ataccttgaa aaattgcatc aaaatgaaga aattacagag gttataagga aacgactttt
     5821 gctagaagct gaaaaggcgg tgaaagaatg atcattggag gaatattttc cggcattgcc
     5881 aactggtggt caggcttatt tggggatggg gatgcagatc ccaaaaccgt aacgaaagtc
     5941 ttacaagatt atggtaatta tctgcagtat caagatgtat tcagttggat ttgtaatact
     6001 tttttatggg gtttgattaa agggttatat aatctcaata ctttactaga aaaaacaatt
     6061 tatcaaagct ttgatttaaa agacttattg aacgctgctg gtgttaatga actgtttcaa
     6121 agtttgatta gtaaggtatt ggctcttttt tgtgtcgtca cattaattta tttaggctta
     6181 aaatttttta tgagtaaaca tccaccaaaa atcaaaaata tcttggttaa tctatttatg
     6241 gccatgattt tgatttttgg tggtagttct ttaatcgatg aagggctaaa tattagtaaa
     6301 aacttttacg gagacgtgac tgcagcaaat aaagcagata aaactggatc acctgctttt
     6361 caaattattc aaaataatgt gatcgatgtg agtacgctac tagaaactga tccgaataaa
     6421 gtagaatccc taccaacaga taaaagaaat gctctcacgg cgaaaaactt taagacagca
     6481 aatattaatt cagtgattat tcctaaggaa gccgctaaga tggcggatga agataagagc
     6541 ggtctatcag atatacaaaa ggatcggcta agagcgttac aataccgact ggaactggat
     6601 gataatggaa aagaaatccc tgtagagatt agtgatgagg ggttagcaaa atatgtgtat
     6661 acttcaggct atcgccgata cattagtagt ccagggatta ttttagctgg tgaatcttct
     6721 ttgtcggttg cctatttatt tatcttgttt acgattgtaa cttgcattat tgagctggta
     6781 tttaaaaaat tctatttagt aattgccgcg tcaacggact ttgagacagg acaacggatg
     6841 aaaagtgcta tagaagatat ttctcaatcc ttcttgttat tggcgtttac gattttagaa
     6901 ttacgaatct atacattaat gctgtcaggt atcggtgatt tgcacgcagc aggcaagatc
     6961 aacggatttt tgtatgttgt cgctttaatt gtactaacca ttgcgctatt caaaggctct
     7021 caatctgtta cgaagatttt cggtgttgat acaagtctga aaaacggcag caattctctt
     7081 cttagcatgt ttgccttagg aaatattgcg aaaaatgtgg gtagtggggc taaaaacctt
     7141 gcaagtgctg gtaagagtgg tttaagcaat ttaaatgata ttcgtaaaaa tggatttggc
     7201 aatcgaaaag atgttattcc tgataacggg gaagaacaac cagacgataa agctacaatt
     7261 gtacctaata gtaagaaaac agtttccgaa atgttcggga aagcgaaaaa tggtgtttct
     7321 aaagccgcag aaggtcttgg ttacgtcaat gaacgtgggt taaaaggaac agctgaagat
     7381 cttacagaaa aagccaaaga caccttagaa gagaaattag acggcacgag agctaaagaa
     7441 ttggctgaag ctgtgaagaa tccatccgaa gcactagaaa aacaaaaaga aaaagcttca
     7501 gaagtcaaag aaggactaaa agatgcgtat ggtgaaggac aagtcaatgc agcagtgaag
     7561 gcaaatacaa caagtgaaaa agaaaatcaa gaaggtgcag cgattccttc agccaaagat
     7621 caccaagatg agttacattc taacgaaaat tcgatcggag aaaaagattc agaagaagga
     7681 caacatggac agttaccaaa aaccgatcca gaacaatatc gttataaatc taacattaat
     7741 tctgaaaatg aaaagcgcat ttcagattta ccaaaacgaa atcaagaagt acaaacggat
     7801 gaggattcca tcgatttgcc aaatgataat ggaatcgaag ttggaactga aaaatcaatg
     7861 aaaattggtt tgcctaagac acaaaattta ccaatgaaag atagtcgttt gaatcaattc
     7921 aaggagaatg atcaaagctt tgttcaccaa tcaactgcag gtactgttca aatgaaacca
     7981 agtcgtttgc ctgaaaatgg aaatctgaca actgtttctt acaatttatc tgaaagtggt
     8041 gagacaggga atgtgtccat tcaaaatgtt gtgacaagag aagctgcacc agaacctatt
     8101 caaaaagttc acacaggtta caaaccagca aatgtggcat ttactgcacc aacaaaaata
     8161 aatgtaacaa gagaaactaa aaacaggaac aatgaattga aagaacaact attgagggat
     8221 atcaaacagg atatcaaacc ataaaaggcc tacgagttag gccttttttc tatgtatgaa
     8281 aaggagaaat catttatgga taaatatatt aacatgaaaa atcctccaag atttagtggg
     8341 gaatggaagt ttttcgcctt tttcttttta gatctattaa cgcttggtgg ttgctttttt
     8401 tgggcgaaaa tagtcaataa tcttgtccac ttaccactct tttttgcact cttgaattat
     8461 ggcttatgga ttgcgatagc tctagtgatt gtttggcgac cttataataa cccaggtcaa
     8521 cgacaaattt ttgcgattat cgaatctatt ttctttgcgg ataagaacca ctatcatgcg
     8581 ttagatccta accgaaatta tcatggtacg tataaggagg atgaataaat gcgagtcaaa
     8641 ggacaaaatt cagttgataa gaaatccatt ttgcagtttg gaaaaccaga agaacgatct
     8701 atcttggatt tatttcctta taaatcctgt acagaagagg gagtcttagt tactaaagaa
     8761 gatcattttc aacgttatta tcgtgtagtt tctacagacg tggaaggatt aaacgaacaa
     8821 gagaaactag aacgaatgaa tcagttgacc accgtcatgc gtacgtatgt gcaaacaatt
     8881 aagttagtta gtttgaccac agagacggat ctaacggaac aaatcgtaca aaagcggcaa
     8941 ttattagata agaatcgtat ggaacaatcc ttaggaagaa atctacagcg attaagaaaa
     9001 tatgaaaaga aattagtaga ggaaatccag gaattgaaac gagcagaaca agaacgtcct
     9061 gacttaagct tctttttcgt cattgaagga aagacaatca aggatttaca aaccagaaat
     9121 cgtcagttaa tgcgatctag tggcatttta gggttaaaac cactctacaa aaaagaattg
     9181 atcaagattc tttatcgaat gaacaatatg aatgatgagt aggaatggag aagagagaat
     9241 gaaatatccg ttgatatggt tagaaaatga tgttccgaag gaagagatca ctagaaaatt
     9301 ttctcacact ttaagcctgg gtaagttggg aaaagggcaa tcttttaacc aagtaaagaa
     9361 tcaaacaaga agaggtgact gtacatgtta gaagcgatta aaacagcttt ttcaccacaa
     9421 aatgatcaaa aagtacttga tccattaaag caactaggaa atcgggaaat agaaatttta
     9481 aaatccaaag ggtatgattt agatttttta aaacaagtga tgccacaagg tggattaaat
     9541 ttcgatgaag agtatgtgac ctatggaaat ggttattcac gatgtgtgac agtctatcac
     9601 tatacaaaaa atcctactct tttttggttg gccaatctca tgaacaatcc tcatacagtg
     9661 gccacactag atgttagcac agatgaaaag caaaaagtga ttgatcaaat caaccgcgca
     9721 ttagatgaat taaatgatcg atcagataat gaacgaaaag cgactgaccg aaataaaagt
     9781 gcggaagaat acctggaact agcggaatat gcccgaaaat tgacttctaa tggggaaatt
     9841 tctaaacaag tcaaagtgag agtacatgtt tacggaaata cgatacaaga agtggatgaa
     9901 cgggcgcagg aactgatcga aaatctaaaa gggatggatt atcgtgcggt gacttatctg
     9961 cataaaacca aacgagattg gcaatcgatg tttaccatgt tcgatgaaca agctgaatgg
    10021 ttcggaaata tcaaagggca atcactacca tcgttaaaca ttggcggagg tttcccgttc
    10081 caccaccaaa cgctaaaaga tccaggaggt acacaatatg gggaaaccat gacaggcggc
    10141 ccatttgtct ggaatgcgac gtataaaaat agtgtacggt tgtctactaa taccttgctt
    10201 ctaggaatga tgggagctgg taaatcgacc gttcttaaaa tgatcgcgga agcccattta
    10261 gctgcaggag actttatttg gggctttgaa aagggaaaag actttattcc tttcttaaag
    10321 gaatataacg gtattatggt gcgtttagac ggatctgacg ggatgatcaa tcctttagag
    10381 atatttgcca cacggacgta tgatgaagca tccaggttat atgacgatgg atcagtcaaa
    10441 gatttgaaga tcaatgaagc agcgtcctat caaacgcact tagacaaagt agtctatcag
    10501 gtacagttag tgagtcctca gctaaaagga acgatgaaag cagaatttaa gacctactta
    10561 aatcgttttt atgaagagta tggaacagta ccacgtggct ttacttcctc aaattcgcgt
    10621 tcaaatactg atacgcaagt cacaggtaaa gatccagaag cttatccgac gttcaaagaa
    10681 tttttagact ttttaggaca actggaatta ccaggtgctt cccaagagaa aaagaatcga
    10741 aaagaagaaa tggaatccat cgtagaatcc ttatgtgaga cgtatggcat gatttttgat
    10801 ggtcacagta cgattcgaca ccttgaccaa caacagttgg tcttttttga cattgactct
    10861 attacaggtt tagatcgagg ggtgcaacaa tgtcaaatgt acatggctat gacgttgatt
    10921 tggaatcagg cattgatcca gggaagaaga caacgacgtt taattaaaga aaaacaaatg
    10981 gaagaacaag acttcaaacg cttcttggcg atgattgatg agtgccataa tgcgattaat
    11041 ccagatttta ttgaaacggt aaattatgtc acaaactttc aacgagaaat gcgtaaagta
    11101 gaagcaatga ctgttttagc tacacaatcc ccacaagaaa tggtgccaga aaatatgtct
    11161 tctgataact tctcgaaact gaagaaagtc tttgaatttt cttctacgaa gatctttatg
    11221 cgtatggatg aatctatttt acgtcatata gaaggactag tgggtaaatc aatgacgaac
    11281 tcagaattag aatccattcc tcaacaaaat caaggagacg ctgtgatcaa ctttggatca
    11341 aaagaaacca ttcgcgttca ttttagtccg aacaaacgtc aattaaaatt gtttgatgga
    11401 ggaaaataat ggaaaatcaa gatgaaagtt taatcaagca gtatgttaag cgtcgcgcaa
    11461 agcggcgctt atttttgtgg ttgtttggta cttctgcagg tttgattact attttgatca
    11521 ctgtgtttgt taccctattt ctaatacttg cggcgggttc gattgataat tcggattcag
    11581 attcaagcag tggtggcgaa gcttttacag gagaatattc ggaaggctta ccgatttata
    11641 aagaaatcaa aggaagaggc cctttttctg atgagattgc gcaatatgcc gtaggtgcag
    11701 cagtgaaata taaattatta ccaagtgtga ttctttcgca atacggttat gaatcagcgt
    11761 ttggtacgtc ggcgtccgca agaaatgatc tcaactattt tgggattact tggtttgatg
    11821 gttgtctgtt tcctaaagga actgcacgag gaattggtgg cattgaaggt ggctggtaca
    11881 tgaagtttcc caactcaaaa gctgcattct cctattacgg ctttatggta gccacacaat
    11941 caaattttaa tgcatgtgta ggaaataaaa gccctggcgc aagcttattg atcttaggac
    12001 gaggtggtta tgccgctgca ggaattactg aagatagtcc gtattataca ggttgtatgt
    12061 cgattattac ctcaaataaa ttaacagaat atgatgaatt tgcgatcaaa cattggggag
    12121 aaggcggaaa tgacaacggt acgattactg gtgaatggac gaatcctttc cctggcagca
    12181 gtttagataa aagttccttc agcggtggtc aattatttgg aacgaatcca ggcggtgaat
    12241 tccgtccgaa tggtttccat gatggattag actttggttc agttgatcat ccaggaagtg
    12301 agattcatgc ggttcatgga ggaaaagtcg tgtacgttgg aaatcctggt atttcaggtt
    12361 tgggcgcgtg tgtgatcgtg attaattacg acggcttgaa tatggtatat caagaatttg
    12421 caaatagtac aggaaattcc cgtgtgaaag tgggagatca agtgaaagtt ggtcaagtaa
    12481 tcgggatacg agatacagca cacttgcatt taggcttcac tcgaatggat tggcgtcaag
    12541 cacaaggaca cgcattcaca gatgatggta catggatcga tccattacca tttttgaata
    12601 gcagcaaaaa atagatcagg agaaaaaata tggaaaataa aacgttaaaa atcgtcttaa
    12661 cagctgcact cagcattagc ttaattggca atatcgcgct cttttggcag ctgaaagagg
    12721 aacaagcgac aaatacctat aaaattgaag aagtggctaa aaacgcgtct gagaagctca
    12781 aaaaaaattt ctctgaggca caggcgaaga tcgacaagtt aaatcaaacc atcaatgaga
    12841 aaaaccaaga aatcgatcag ctgaagcaac aagacggtat aaaagcaaaa gaagaagtca
    12901 cagatgccca aagaaagacg gcagaagagt tcggtcaatt ggccattgat aaatcactaa
    12961 gcagaaatga attaacggaa aaactaaaag atgtcgcaac ccaagaagtg atcgacaaat
    13021 taagtcctgc agatgatgat caccaacatg atgattatgg aagttactcc tttacattag
    13081 gtgacgtgca aacttacgct gaaacaagca gtgtaaaaga ggaacaaaat tttgtcgtat
    13141 ttgttgatta tacaatcggc aatcctcaat tcaaagatgt caaaccgcaa aaaataaaag
    13201 gtggattaac tgtcactgaa aaacaagtag atggtcaatg gaaagtcaca gactttagtt
    13261 atttcactcg ttaggaggaa gaaaatgagt aagaaaaaag gagcaagaca acgttttaaa
    13321 aaacggaaaa gttatcttag atggctggga atctttacga ttctcggctt tttgattctt
    13381 gctagtccga tttatacgca taagaaggta aacataccag atgaggtaag cacgaacgct
    13441 tttttaaaca cgaaagaact gacaatggta tcaaaagaaa aaggagcgaa tcagcaatta
    13501 gtttttgaat ttgcgattga cgatgaggat caaggcttat taaaagaact ggctaatctt
    13561 gattatcgcg tagaagtaaa gacagctaaa ggtgattatc aagcaataaa agccaaagtg
    13621 atcaaggtgt ctgatgatta ttttgtagtg aaattagcac atgtaccaga agaatatatc
    13681 gccatgcgtt taaccattgt tcctgaaaaa attgatccaa aagtggatat gcaagaacca
    13741 caagatctga tttattacgt tcacgaagat aaggttaagg atcaagtgaa agatgataat
    13801 tatgaacaac atgcgatcaa ttacaaagtg aaaggataca agaaagaaca aaaagcagca
    13861 caaaaacaga ttgaatcctt gcaagcaacc attgatttga atgaagaatt agtgaataaa
    13921 ctaaaagacc aattgcctta tcaagtcgca gatgatcagg aaaatactga aaataagatt
    13981 aatgattacc agcaagaaat tgaaacatcg aaaacgcaaa tgaaagatca agaagagatc
    14041 attcaaaaaa ttcaagaaaa aattgatcgt atgacaaaag aaaatattta gtagaacagg
    14101 tggagtgaaa gaaatgaagg gagcgaactt ggtcggcaaa gatattttcg ctgagcagaa
    14161 agtattgatt aaagcctatc tcaaagttgt aaaagctaaa gaaatatgtc aagaagtgaa
    14221 tttatctaac acaacaatat ctgagttgaa gaatggtaga aagagtttag ataccttaaa
    14281 attcagatca tttgaagctt tgtatttagc tgctaaaaaa tattttacgg ctgaaagaaa
    14341 ataaaaacag gaggatttta tgt
//
LOCUS       DACEUF010000067        14094 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.070, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000067 DACEUF010000000
VERSION     DACEUF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.070"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..1412
                     /locus_tag="IXG20_001981"
     CDS             <1..1412
                     /locus_tag="IXG20_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1610173.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..2889
                     /locus_tag="IXG20_001982"
     CDS             1546..2889
                     /locus_tag="IXG20_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1610174.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            3001..4452
                     /locus_tag="IXG20_001983"
     CDS             3001..4452
                     /locus_tag="IXG20_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1610175.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4801..5433
                     /locus_tag="IXG20_001984"
     CDS             4801..5433
                     /locus_tag="IXG20_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ1610176.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            6021..7460
                     /locus_tag="IXG20_001985"
     CDS             6021..7460
                     /locus_tag="IXG20_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1610177.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7755..7973
                     /locus_tag="IXG20_001986"
                     /pseudo
     CDS             7755..7973
                     /locus_tag="IXG20_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8222..8437
                     /locus_tag="IXG20_001987"
     CDS             8222..8437
                     /locus_tag="IXG20_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610178.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8654..8910)
                     /locus_tag="IXG20_001988"
                     /pseudo
     CDS             complement(8654..8910)
                     /locus_tag="IXG20_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(9097..10008)
                     /locus_tag="IXG20_001989"
     CDS             complement(9097..10008)
                     /locus_tag="IXG20_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1610179.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(10027..11031)
                     /locus_tag="IXG20_001990"
     CDS             complement(10027..11031)
                     /locus_tag="IXG20_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1610180.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(11028..13151)
                     /locus_tag="IXG20_001991"
     CDS             complement(11028..13151)
                     /locus_tag="IXG20_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1610181.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13156..14076)
                     /locus_tag="IXG20_001992"
     CDS             complement(13156..14076)
                     /locus_tag="IXG20_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1610182.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         4605 a         2506 c         2650 g         4333 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag
     2701 agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt
     2761 tctcttgcta tagaagaaca aattacatta gacaaattga aaatgttaga ttatttattt
     2821 catcctagtt ttagccaacc ctataatttt attttaaagg cagtaacagg attaaacatt
     2881 gaaacataat cgttactaaa tgaaatcaag aaatatactg tattactcaa ttcattgaaa
     2941 atatttatca taatttttaa tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa
     3001 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
     3061 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
     3121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
     3181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
     3241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
     3301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
     3361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
     3421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
     3481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
     3541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
     3601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
     3661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
     3721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
     3781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
     3841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
     3901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
     3961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     4021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     4081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     4141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     4201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     4261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     4321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     4381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     4441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     4501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     4561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     4621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     4681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     4741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     4801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     4861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     4921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     4981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     5041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     5101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     5161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     5221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     5281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     5341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     5401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     5461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     5521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     5581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     5641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     5701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     5761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     5821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     5881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     5941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     6001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     6061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     6121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     6181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     6241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     6301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     6361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     6421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     6481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     6541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     6601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     6661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     6721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     6781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     6841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     6901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     6961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     7021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     7081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     7141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     7201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     7261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     7321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     7381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     7441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     7501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     7561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     7621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     7681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     7741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     7801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     7861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     7921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     7981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     8041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     8101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     8161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     8221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     8281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     8341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     8401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     8461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     8521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     8581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     8641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     8701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     8761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     8821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     8881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     8941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     9001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     9061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     9121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     9181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     9241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     9301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     9361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     9421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     9481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     9541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     9601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     9661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     9721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     9781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     9841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     9901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     9961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
    10021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
    10081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
    10141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
    10201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
    10261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
    10321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
    10381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
    10441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
    10501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
    10561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
    10621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
    10681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
    10741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
    10801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
    10861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
    10921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
    10981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
    11041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
    11101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
    11161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
    11221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
    11281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
    11341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
    11401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
    11461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
    11521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
    11581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
    11641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
    11701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
    11761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
    11821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
    11881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
    11941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
    12001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
    12061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
    12121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
    12181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
    12241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
    12301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
    12361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
    12421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
    12481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
    12541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
    12601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
    12661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
    12721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
    12781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
    12841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
    12901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
    12961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    13021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    13081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    13141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    13201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    13261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    13321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    13381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    13441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    13501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    13561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    13621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    13681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    13741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    13801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    13861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    13921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    13981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    14041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc cacc
//
LOCUS       DACEUF010000068        13809 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.071, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000068 DACEUF010000000
VERSION     DACEUF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.071"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="IXG20_001993"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="IXG20_001993"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ1610183.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="IXG20_001994"
     CDS             complement(2262..2513)
                     /locus_tag="IXG20_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ1610184.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="IXG20_001995"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="IXG20_001995"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ1610185.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="IXG20_001996"
     CDS             3463..3681
                     /locus_tag="IXG20_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610186.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="IXG20_001997"
     CDS             complement(3970..4482)
                     /locus_tag="IXG20_001997"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ1610187.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="IXG20_001998"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="IXG20_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ1610188.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7369)
                     /locus_tag="IXG20_001999"
     CDS             complement(6935..7369)
                     /locus_tag="IXG20_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ1610189.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="IXG20_002000"
     CDS             complement(7379..8167)
                     /locus_tag="IXG20_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1610190.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /locus_tag="IXG20_002001"
     CDS             complement(8164..9105)
                     /locus_tag="IXG20_002001"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ1610191.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="IXG20_002002"
     CDS             complement(9282..9869)
                     /locus_tag="IXG20_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ1610192.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="IXG20_002003"
     CDS             complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="IXG20_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ1610193.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11691..12701
                     /gene="gap"
                     /locus_tag="IXG20_002004"
     CDS             11691..12701
                     /gene="gap"
                     /locus_tag="IXG20_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1610194.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12786..13721)
                     /locus_tag="IXG20_002005"
     CDS             complement(12786..13721)
                     /locus_tag="IXG20_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1610195.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3807 a         3096 c         2436 g         4470 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcgtaattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgaa aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgaggat gccgtttcac agcaccccta ataaaggagg aacttttgat atgacagtaa
    11701 aagtaggtat taacggattt gggcgtattg gccgtctggc tttccgccgc atcaaagaag
    11761 tctcagatga tattgaagta gtagcaatca acgacttaac aagtccaact atgttagcac
    11821 aattattgca atttgactct actcatggta cttatcctgg cacagttact gcaacagaag
    11881 actcaatcgt agtggatggc gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa
    11941 tcccttgggc aaaagaaaac ggtgtagata tcgtactaga atgtactggt ttttatacat
    12001 ctgaagaaaa agcaaaagcg catttagatg ctggtgtcaa acgagtagtt atttctgccc
    12061 cagctggtgc tatgaaaacc atcgtctata acgtcaatga tgatacatta gatgaaaacg
    12121 acaaaatcat ctcagcaggt tcatgtacaa caaactgttt agcacctatg gcttatttct
    12181 taaataatga atttgggatc gaagttggta caatgacaac agttcatgcc tacacttcca
    12241 cacaaatgct cttagatgga cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg
    12301 ctgacaatac tatcccacat tctactggtg ctgcgaaagc aatcggctta gttatccctg
    12361 agctacaagg aaaattacaa ggacacgcac aacgtgttcc agtcgttgat ggttcattaa
    12421 ctgaattggt ttcagtattg aaaacaaaag taactgctga tcaagtaaac gaagcaatca
    12481 aaaaacacac agttgacaat ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg
    12541 atgtcattgg aacaactcaa ggttcgatct ttgacccaac gcaaacagaa gtaacaactg
    12601 ctggtgattt ccaacttgtt aaaactgttg catggtatga caatgagtat ggatttactt
    12661 gccaaatgat tcgcttacta gaaaaattcg ctaacttgta atttgataga tcaatcgata
    12721 aaagaagcct ttgactggat ccagtcaaag gcttctttct tttcatcccc tacttttaaa
    12781 ctcgttcaat gaacatattg gcttaaaaat tgttggacta cttgctgata tttttcagga
    12841 tctgtcttgt atgcttccgc atgctcggct ccttttacaa catacttttc ttttggtccc
    12901 ttcgttgcac gatagacctt atctaacatt tcaaatggta caaatgtatc ttcgtctcca
    12961 tgtataaaga gcattgggcg ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg
    13021 aaaaagtagc cagcacggac ttttgtcact agactcgtta cttgcataag cggaaatgct
    13081 ggaagactaa acatatcgtc tagctgataa gcaagctcat catgaacgga agaataaccg
    13141 caatcttcaa tgattgcttt cacattatct ggtagttttt ctccgctagt catcataact
    13201 gttgctgctc ccatgctgac accgtataga acgatttctt gcgattttcc attgttttct
    13261 agcactttgt ttatccactg tacatagtct ttacgctctg gccatccaaa tccaatgtaa
    13321 tccccttcgc tttttccatg tcctcttgca tcaggaacaa ggacattgta acccatatca
    13381 tgaaacatct ttgcgtaatt ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca
    13441 agagctgttt tttcactttt cgtttcagca ggaagataaa tcgctgataa tttcagaccg
    13501 tcttttgaag tgatggtcca atcgcttcgg ttttcttttg aactaaacca tttatgtgca
    13561 tccgtttctt tggatgtctg tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca
    13621 gaaggcacaa cagcataatt ataaaaataa ttccctgcaa acactaacgc aacaatcaga
    13681 gctaaagcga cgcctaaaag gattttcatc caaagtttca taaatcatcc atctcatttc
    13741 tcttttttca tataaccttg aattagtata atcaaagaat ctctactatg caatgttcat
    13801 ttttataac
//
LOCUS       DACEUF010000069        13666 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.072, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000069 DACEUF010000000
VERSION     DACEUF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.072"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            4..360
                     /locus_tag="IXG20_002006"
     CDS             4..360
                     /locus_tag="IXG20_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610196.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            376..726
                     /locus_tag="IXG20_002007"
     CDS             376..726
                     /locus_tag="IXG20_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAQ1610197.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            755..1534
                     /locus_tag="IXG20_002008"
     CDS             755..1534
                     /locus_tag="IXG20_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAQ1610198.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1545..2222
                     /locus_tag="IXG20_002009"
     CDS             1545..2222
                     /locus_tag="IXG20_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAQ1610199.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2241..3347
                     /locus_tag="IXG20_002010"
     CDS             2241..3347
                     /locus_tag="IXG20_002010"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAQ1610200.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3337..4428
                     /locus_tag="IXG20_002011"
     CDS             3337..4428
                     /locus_tag="IXG20_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAQ1610201.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4441..5181
                     /locus_tag="IXG20_002012"
     CDS             4441..5181
                     /locus_tag="IXG20_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAQ1610202.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            complement(5351..6538)
                     /locus_tag="IXG20_002013"
     CDS             complement(5351..6538)
                     /locus_tag="IXG20_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ1610203.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            7332..7526
                     /locus_tag="IXG20_002014"
     CDS             7332..7526
                     /locus_tag="IXG20_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610204.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            7717..7966
                     /locus_tag="IXG20_002015"
                     /pseudo
     CDS             7717..7966
                     /locus_tag="IXG20_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8210..9040)
                     /locus_tag="IXG20_002016"
     CDS             complement(8210..9040)
                     /locus_tag="IXG20_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ1610205.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            9248..10582
                     /locus_tag="IXG20_002017"
     CDS             9248..10582
                     /locus_tag="IXG20_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1610206.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            10623..11534
                     /locus_tag="IXG20_002018"
     CDS             10623..11534
                     /locus_tag="IXG20_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1610207.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            11531..12385
                     /locus_tag="IXG20_002019"
     CDS             11531..12385
                     /locus_tag="IXG20_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1610208.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            12382..12999
                     /locus_tag="IXG20_002020"
     CDS             12382..12999
                     /locus_tag="IXG20_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1610209.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            13001..13519
                     /locus_tag="IXG20_002021"
     CDS             13001..13519
                     /locus_tag="IXG20_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1610210.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         4185 a         2379 c         2898 g         4204 t
ORIGIN      
        1 atcatgatta aagtagttat tgcagaccgt atgggtaaag gacaaaatgt ggccaaaggg
       61 gtagaagcag ctggtggaaa agcagttgtc gtaccaggaa tgggggcaga tatgcgtttg
      121 ggcgatgtga tgcaacaaga gcatgcagat atgggtatct ctttttgtgg tagtggtggt
      181 gcaggtgcat taacagcagc aacaaaatat ggctatccag aacgacatgg tatgcgttcg
      241 attgaagaag ggatcacagc tatcaaggat ggcaaaactg ttctaggttt cggctttatg
      301 gatcaagagg aattagggaa acgtctgaca gaagaattta tcaaaaagca tggaaaatag
      361 aaagagggac atgaaatgag cgttcaaaag acaattcagc aaaaagttat cgtctccggt
      421 aaaggggaaa caaagcaaca tgcttttgca tcagcgttag cagaaattca aaacaaaata
      481 atggaacaat caaacgtatt attgcgaatc gaaccagtca atgtggatat tttaaaggcg
      541 gtcgaaaaca aatacacaga acgctttcta tttttcttct tcccacggca acgtattagc
      601 tatgaagtcg aactctctgt tactgtggct gtaacagaaa taaaattggc agacatatcg
      661 ttcagccagc aaatgactga agatccaaat gggattgcca ttccatttgt aactaaaaaa
      721 atctaatttt gaatcataag ggggaaagaa taacgtggat ttcgtaatca ttctagtcaa
      781 atcggtcatt atcgggggat tacttggttt tgcagcatct gtcggggcag ctcgaatgtt
      841 tcatgcacct aatacacaag gtttaggagc ttttagaaca ttaggcgaaa tgaatgcctg
      901 catgggtgat ccagcttctc atttttcatt tggtttaggc tttttcttta atgcatgggc
      961 ttcttcagtt ggtgctggtg cattcacaca agatgtgacg catcgaatca ttcccaattg
     1021 ggcagcggca gcacttctag caaaaaacaa agatgtcacc cagaccatgc atgatccgaa
     1081 aaaaatgggg atcattggag cggttatcgg ggcagtggtg gtagccttct taaacacaac
     1141 agctgcagca attccagagg cgttacaagt gacggcggtt tccgttttgg taccagcagc
     1201 gacgattctg atcaatacag taatgccagt tattttttgg ttggcagctt tagacgcagg
     1261 gaaacgaact ggtttttggg gcactctttt tggtggtatc gcgcaattga ttatgggaaa
     1321 tgcggtacct ggtgttgttc taggaatctt gattggtaaa ggcgtcgatg agagcggttg
     1381 gaatcgcgtc acgaagagca tgatgatcgc tattgtttta ttatttattt tcagtggatt
     1441 tttccgtgga tttgatatgg aaatgatcaa gagcttccag cttcaagtgc ctaattggct
     1501 tgataacttg cacaacatct ttagtgtgaa ataggaggag aatcatggag acaacagaag
     1561 aacaagcaaa agtattagaa gcaaagcggc aaaagagctt ttggtttgca gactggtctt
     1621 ttccgctaat cgtaggaatt atgtccgctg ctgtatttgc aggaacacac atgtatgtga
     1681 tgtatggtgt gggtgcattc aatgaagttt cgatcgtggc gatgttaaaa gccgggctgg
     1741 atggcggatc ctatggcgca gcggcagcgt ttggtgctag tttcttattt gccagaattt
     1801 tagaaggctc tttagttggg atcttggact taggcggttc gatcctaact gggatcggaa
     1861 ttggggtacc agcaattctc ttaagtatga aaatagcggc accaatcgat aattttgcac
     1921 tctctttatt gactggtttg gttctaggtt tgattgttgg tggcatcatt gtcttgattc
     1981 gtaaatttac gatcaatcaa tcaaattcca cttttggtgc tgacgtcatg atgggggcag
     2041 gaaattcatc tggacgtttt ttgggtccat taattatatt aagcgcgtgt gcagcatcta
     2101 ttccaatcgg cattggcgct acattaggag cactaggatt ctatgcttgg aaaaaaccaa
     2161 ttgctggagg tgctatttta ggagcaatga ttttaggagc aatcttcccg gcagtaaatt
     2221 aatagaaaga gggtcattaa atgtttgata ctttgattaa aaatggaagg ttagttgatg
     2281 gatctaaaat agaggtcgct atagaaaaag gcatgattaa agcagttgca gcagaaataa
     2341 atcagccggc gcaagaagta gttgacttgg agggaaagta ttatttatcc gctgggtgga
     2401 tcgatgatca tgtccactgc tatgaaaaga tgaatcttta ttatgactat cctgatcaga
     2461 taggggtcga aaaaggtgtg acgacggtca ttgatgctgg aactacggga gcagaaaaca
     2521 tccgtgaatt ttatcaattg acgaaatcgg tcaagacaaa tgtgtatgcg ctaatgaata
     2581 tctctaaatg gggcattgtc aaacaagatg aacttgctga tctaacaaag attcaagaag
     2641 atctagtaag tactgcgtta gcagaactgc cggaatttat tgtcgggatt aaagcgagaa
     2701 tgagtaagac agttgttggt gaaaatggca ttaagccatt agaattagct aaaaaaatcc
     2761 agacagaaaa caatgatctg ccattgatgg ttcatatcgg ttctgcacct cctgagttag
     2821 aagaagtatt gtcaagaatg gaaagaggcg atgtattgac ccattgtttt aatggaaaac
     2881 caaacggtat tttagatcca aagacagatc aaattaaaaa ctttgtctgg gatgcttaca
     2941 ataatggtgt cgtctttgat attggacatg ggacggatag ctttaatttc catgtggcag
     3001 aaaccgcact aaaagcagga atgaaagcta cctctattag tacggatatc tacatccgca
     3061 atcgtacaaa tggcccagtt tttgatctag caactacttt agaaaaattg agggtagtcg
     3121 gctattcttg ggaagaaatc attgaaaaag ttacagcagt tcctgcacgg aattttcatt
     3181 tgaagaataa gggccagtta gctgtcaatt atgatgcaga tttgacgata tttgagattg
     3241 ttgaaaatga aaaggaattg aaagattcca acggatttac acgtatagct aaagaacaaa
     3301 ttttaccgat taaaacaatt attggaggcg tcatatatga caattagcta tggaaagttt
     3361 gatttaaaag aagtcattaa tgcatcggga aaaatgacga tcctaggggt ttctaaggta
     3421 tctgaaagtg tcctagcagc acaacgattt ggaggcgagc atttttttga gatgagtgat
     3481 cttgccatca aaaccggcgc ttatctttct caattactga aagtggaaga tgcgcaaatc
     3541 gtttcttctg cttcagcagg gattgctcaa tctgttgctg ctttaatcgg acagggcgac
     3601 atgtaccatg tctatcatcc ctatacagaa aaaatcgcta gacgggaaat catcatacct
     3661 aagggacata atgtagatta tgggacaccg gttgaagtaa tggttgctca aggtggagga
     3721 aaagtcatag aagctggcta tgcaaatatg tgtacaccgg aacatgtttc catgatgatt
     3781 acggaaaaaa ctgctgcgct tctatatatt aaaagtcatc atgcagttca gaaaagtatg
     3841 ctgacggtgg cagaaatggt agaagttgcc aaagctcatc gtttgccatt gatcgttgat
     3901 gcagctgcgg aagaagattt attcaaatat agcgaaatgg gcgttgactt agtgatttac
     3961 tctggggcaa aagcaatcga aggtccgagt gccggactgg tcataggaaa aaagaagtat
     4021 attcaatgga ttcggctaca gtctaaaggt attggccgag caatgaaaat cggtaaagat
     4081 aatattttag gctttaccca ggcactagaa gattatttaa aaaatgggag cgaatccggt
     4141 gactccatga agcaacgatt agcgcctttt gttaacgcaa tcaatcaact tcctgacctt
     4201 tcagcgaaaa tagttcaaga tggtgccggc agagatattt atcgagcaag tgtaacgatt
     4261 aatggcgata aaagtgcgaa agaagtcatt caggaattaa aagcacgaaa tccagcagtc
     4321 tacacccgag aatatcaagc aaataacgga atcattgagt tcgacattcg ttccgtcagc
     4381 caaaacgaaa tggaaaaaat tataaaaaga ttgagagaaa tcatgtagga ggcttttaaa
     4441 ttgtcagtta tcccaaatta ttataaaaat cgtgtatgtt taaatgtatt agctcattca
     4501 gttgaaaatg caaaagcatg ctatgaagca gcggaagggc atgtcgtact aggtgtgcta
     4561 tcgaaaaatt atgctagcga tgaagcagca atcgaagata tgaaaaggta ccaagacgca
     4621 accaataatg cgttgtcagt tggattaggc gctggagatc caaaccaaag tcagatggtt
     4681 tcgcgtttat ctggggtttt acaaccacaa catgtcaatc aagtatttac tggcgtgggg
     4741 acttctcgtg ctttattggg ccaaaatgat acagtcatca atggattggt ttcaccaaca
     4801 ggtaagatcg gctatgtcaa tattgctaca ggtccgttaa gttcacaagc gccagcagct
     4861 gaagtgccta ttgaaactgc cattcaaatg ctgcaagaca tgggaggtac ttcaatcaag
     4921 tatttcccga tgaaaggctt agcacatagg gaagaattta aagcagttgc ggaagcttgc
     4981 gcaaaatatg aattctattt agagccaaca ggaggaattg atctttcaaa ctttgaagaa
     5041 attctgaaaa ttgcaatcga tgcaggtgta aaaaaagtga ttcctcatgt ttatagttca
     5101 attatagata aagaaacagg agatacgcgt ataggagacg taaagatact attaagcatg
     5161 gtaaaaaaaa ttattcacta agtcaagtcc acaatcttgt gtaaaagttc aacctcatca
     5221 aaagcacaat gttttgcgtt gtgaacctaa ctgccatgcc tttctttctg aggagagagg
     5281 tcaggagcag tagcgcagcg actcttgatg tctctcctca gaaagaagag actcgcacag
     5341 gttcatggtg ctaaatgtta ttcgaattaa attctaaata ttttcgtgga gaagccagcc
     5401 actcagtgtc tttttccatc aaaagtgttc caattatccg agtgatggaa ttgatatttg
     5461 gaaaaatccg aatcactcgt tctctccggc gaatctcttc gttcaaccgt tccaacacat
     5521 tggttgtacg aatccttcgg cggatatttt caggaaaaga caaaatttga atagcatctt
     5581 caaagccgtt ttctaaaatg tcgcaagcag cacttaattt tgaatcacaa ccatattttt
     5641 ccaagaagtg ctcttttctt tcacgtgtca attcaagttc agaggcttta aagatactct
     5701 tcaactcgtc ttttacatca gaagaaactt tctttggcag cttatcaaaa atatttctca
     5761 aaaagtgggc ttggcatctt tgccagctcg cacctaaaaa gttcttgcga acagccttaa
     5821 ctaacccttt gtgtgcatca gaaatgacca gttttacacc gaacaaacct cgttgaacga
     5881 acgattgaaa aacggtatcc caattatctt ccgattcgcc gtcttgaaca tcaaaaccaa
     5941 gaatcgtacg tcgtccatca gaatcaatgc cgataccaat atatacacct tttgagacaa
     6001 tacgatgatt ttcccttact ttcatataca gggcatcaca catgaaaaat ggatattccg
     6061 agccttctag gctccggttt ctccactcaa aaacaaatgg atccaactgt ttcatcagat
     6121 ttgaaacgaa tgatttagaa acagtcgcac catccgttag cagttcaact gttttagtta
     6181 ctttgcgagt ggagacacca gaaatgacca tttcaatcat ggcagcgatc aaagcttttt
     6241 cgcttcgctg atatctttca aagatagtag gactaaactt accgtctctg gttcgtggaa
     6301 catgaagttc taacgtacca attcgagttg taaacgaacg atcgtagtaa ccatttcttt
     6361 gtgattgccg ttcttctgaa cgttcatagg catttgcctg aatatattga tttcgttgtt
     6421 cttccatcag ctgattaaac atgatagtca taagtgtttt tgcggtatca ttcgcaccgc
     6481 tttcgtttat aattgcttga atttcttcgc tttcaaaagt aaaatgtact tgagtcattg
     6541 atatcagtcc tattcgagta tgttttctgc aaaaaacatt gtactcctaa ctgagatcaa
     6601 tggctttttt gatgcttcac ttttacacaa ttatatggac gtaatctaaa aattctaaag
     6661 gattcctaac catcttatta gcttttatag ggtatatgtt ggttgggctg ctaaaatcat
     6721 acagtaatga gttgctgaat ttttcaacat ttattaatga tacattagtt ccaagtttgt
     6781 ttttcattgt attttttgct gttggttatt ttataataaa gatatgatga ctaatttagc
     6841 tcaactattc cagcgtcagc tgaagatatt gcaattcccg attccttaat catttatctg
     6901 gatgagacag aaagtgaagt tgcaacagat gtgcatgtag ttgaggataa aaatgatagt
     6961 ttaaaaatta atagttcaga tactttacta agagtaagtc ctatcttttc agctgttatt
     7021 tagatgcaag tagcaactaa gttaggtagt actcctgcaa tggataggta tttgtgtgtt
     7081 cgatatataa gtggtcgaag tatcaaggtt atgtatactg gatgggagat agaagagtta
     7141 aaaattggag gtcaccgcca caaatggaat ttagatttag aggtacctta gatttggtat
     7201 agttattaaa cacaagatgg cctggtttgt agggaaagtc gaagagtatt tttccacttc
     7261 tgcgcccctt taatttttct agtaatgccg tgaatttgtg attaattgga aatatgaaga
     7321 aaggagagtc tatgaaaaca acactagttc tattctataa aaagcatcct tattttacac
     7381 ttctgataaa tatattgttg gctagtgtta tcgggatttc tgttgaatat ctaataaata
     7441 aagatttcat aggttcttgt ttttatacgg ctttatttct tggacttctt gaagcatttt
     7501 ccatttataa aaaatcaaaa aaataaaata gattgccgta agagatcttt ttcatttcat
     7561 ttttttctga aaacccgttg tttctgtcag tgaagcagcg ggtttttaga tttattgtct
     7621 ttagacctaa ttgagcgcgc cgagcgattg aaatacaaaa ccacttgcta tgcgggtgga
     7681 gcaataaagg gaaatagttt agcccgtaca aagtagatgt gtaaatacca cattgtattc
     7741 acaccaaagt atagaagaaa aataatttat aatcaatatc gagccaatat tcaggagatc
     7801 atcaagagat tgtgcaaatc taaaatataa atttgggtat cgacactttt gggtggaagg
     7861 atactatgtg acaaaagtag gacaaaatga agcgaccatc aaaaagtaca ttcaagaaca
     7921 agaaaaacat gatagtgcgt tagataagct gagtgtacga gagtaagacg atccttttaa
     7981 gggctaagga gtagtacata aaccttttaa gaggtgggca aaactggcaa aggcaatatg
     8041 gcttgaacat aaaatatgaa agccagcgtc tttaggcgct ggccggtgat ttgagcttat
     8101 agctctagag aaaaccaccc ttttgaaggg tggttatgat taatgacttt agtcagtttt
     8161 gctcacaaag cgtgtggaac aaaaaacggg tgtttctacg ggtagatatt tatttcttga
     8221 atggaggtgt cccatacatt tttggtgtta ctggttctaa ttttggtgca gcgccttcga
     8281 tcttaggttc tttcaaccat tgatattcgc cttcgccatc cagtgctttg ccactagccc
     8341 aagaaccttc actactttgg tctccctctg agaagttata gaagtcataa gcaatgtctt
     8401 ttcgttcttt gtcacgcggg aaagtacttg ggactaagat tgaaccttct ttttcttcta
     8461 attctttcac tgcacattcc catagatttt gatgatagct atctcttgca aggagaacag
     8521 acagcatatc ttttacgcca cgatcatcga tcatttcgta aatacgtgct acttgaagtc
     8581 ttccttgtga ttcggcattt aaattagctc taaagtctgc taacaagttg ccactcgcaa
     8641 tgatataggc accgctccat ggattgccca cgctgtcagc aggacgaggt cctaagccag
     8701 ctacgatcgc gtgctgtgga ttcattccag agataatggc accgattgtt ggatcttttt
     8761 gcatcgctgc ttcttgatct tcaacaggcg caccctctaa taaacgagca accattgtcg
     8821 ccaacatttc cacgtggccg atttcttctg ttgcaatgtc catcagcata tctttatatt
     8881 tttcttctcc tcggcagctc cagccttgga acaggtactg catagctaca ctcatctcgc
     8941 catattggcc gccgatcagc tcctgcagat atttcgcgat taatggattc ggacgatctg
     9001 gttttgcttc aaactgcaat tctttctggt gtctaaacat caaaattcct cctaatctat
     9061 ttttaataca ttctcattct gctacgaaag attaaaaaat tcaaagaaag cgctcgcgaa
     9121 ttatataaag tattggtatc attaataatt tttttagaat cataccttat aaattgttat
     9181 tttcagaaca aaatgatatc gttatattaa aaccggcatc ggttacatta aaatttggag
     9241 gtaaaatatg aaaaaattat tgtttacagg gttgctggca agttcagcat tgtttttatt
     9301 aggaggatgc ggatcaagta atgcaagcga tgccagttca agtgcagcaa attcttctga
     9361 aaaaacaagt gatggaagaa aaaatattga tttctggtcc ttttggggat ccggtgcacg
     9421 aaaagaagct atcgaagaaa tcattgagga tttcaatcaa tcacaaaacg agatccatgt
     9481 aaattataag tatcagccat ggggtgacat ctggactaaa tctttatctg caataacagc
     9541 tggaaatgca cccgatgtta ttgtacagga cataaattcc gttgcccaaa gagcagaagc
     9601 acagcaagca actaatctct ccaaatatgt agaaaaagac acttctaagg atttttatcc
     9661 gcagctttgg gatacagtag aatatgaagg tgatccttat gcgatcccat tcaatacaga
     9721 tacacaagtc attttttata ataaaaagtt atttaaagaa gctggaatca gtgaacaaga
     9781 tctaccgaca acttgggatg aattagaaga aacagcacgt aaactagata aaaaagaagg
     9841 aaaagaattt acacgcatcg gtttttatcc attatggaat ttaggggctg atgtatgggc
     9901 gctaaacgca gataacggga ctagttggtt tgacaaagat gaaaaagtca agattgatac
     9961 taaaaataaa gtggaagcct tggagtggat ccaagactgg caagattatt atgggaaaaa
    10021 tacgatcaat aaattagaag ccgaatttgg gtctggtgta tctgatccat ttatttccgg
    10081 attagtagcc atgcgtgccc aaaatatcaa ttattactct agtttgatgg aaaatgcacc
    10141 ggatgatttt gattttggcg tgatgcagct cccagaaaaa gaagaaggtt caggccattg
    10201 gtcgtggggt ggcggattcg tcttggaagt accatacgga gcaaaagatc ctgaggcctc
    10261 ttatgaattc atcaaatatc tttcttctcc agaagtacaa gaaaaattcg gcgaaaaaag
    10321 ttttgatatc atggcgagca agacagccaa tgagaattta gtgaataatg acaacctaac
    10381 ggataaagga aaaatgatct atcaaatggc ggacgaaaac tttaaaaata cggtgatcac
    10441 accagttcct ttagatgctc cggattacac gacattagtg aatgaacaga ttgaccagat
    10501 tcttttagga acaaaatcac caaaagaagg attggcagat gcacaaaaag cagtagaaaa
    10561 cttagtcaag caaaatcaat aatccatttt tgactgtcta ggtacagagg aggcaagaaa
    10621 atttgaagcg tttatctatc cgaaagaaaa aagaagcaag atgggcctat ttatttatct
    10681 cgccttttat tatcgggttc ttggtattta tgctagggcc tatgcttttt tcagttattg
    10741 gtagtttcac cgattataat ttgacatcca agatgaattt cattggtcta gcaaatttca
    10801 aaagaatgtt cgttcacgat gatttatttt ggaaatcgtt atacaacacc gtttatttcg
    10861 tcatcttcaa tgtaccgctg acgacgattt tcagtgtgtt tttagccaca gtactcaatc
    10921 aaaaaatcaa aggaatcagt gtattccgta cagctttcta tttaccggca gtcttatcag
    10981 gagtagcagt ctacatctta tggatgcagc tgctttctcc atctgcagga ttgatcaaca
    11041 catttctggg ctggttccat atccctggac cagcttggct gtttgatcca caatggacga
    11101 aacctgcgtt gattttgatg aaaatatgga gttctggagg agcaatgcta ctttacttag
    11161 ctacattaca aaatgttcct agatctttat atgaatcagc agaaatcgat ggagcaggcg
    11221 tttggggaaa gttcaaaagt attactttgc cattaatcac tcctattatt ttttacgatg
    11281 tgatcacctc tacgatcggt tctttccaaa tttttcaaga agcttatgtc atgacgaaaa
    11341 atggaacagg cggacctgca aattctttgc ttttttataa tctgcatatg tggaacaagg
    11401 cgttcgtagc ttttgatatg ggttatgcga tggctatgtc aatgatcctg tttctgatcg
    11461 tgttggttct gacgtttgtc aatttgaagc ttgcacctaa gtgggtctat tattctggag
    11521 gtgacggcaa atgatggatt atttcaaaaa gaccagtgtg aatcggcaaa caaaaaaaat
    11581 actaagttat gtcctcatga cagtaatagg aatcattttg atcattctat tgctttggat
    11641 ggtgtttact tcattgaaac caatggaaga aatcgttcgt tatcctccga cctttttccc
    11701 agaaaaaatc gtatgggaaa actatctgga tacgattgcc gcctttcctt tttggcgcta
    11761 tgcaagaaat acattattca tcactgtgct agtagtgatc ggtaatgtcc tttcaaattc
    11821 ttttattgct tatggatttg cgaagcttga ttttcctggg aaaaagctga tgtttgcttt
    11881 ggttctttct acgatgatga tcccggggtt tgtaacgatg atccctcaat atgtattatt
    11941 ctccaagatc ggctgggtag gtacttattt gcccctgatc gttccatcgt tcttcggcaa
    12001 tgcgttcaat atatttctga tgcgtcaatt ttacttatcg atcaataatg aattgatcga
    12061 agctgctgaa atcgacggag cgaatcattt gtatatttgg agtcatttga tgcttccatt
    12121 gacgaaacct gccttgatta ccattgcgat caattccttc aacgcagctt ggaatgattt
    12181 cttaggtcct ctgttatata tccaagatca agaaaaatac acgttacaga ttggcttgca
    12241 agtttttcag aaccaagcaa cgacacaatg gaattatctg atggcaggag caacacttgt
    12301 cttgatcccg acgattttgc tgttcttttt tgcacaacgt tatttcattg aagggatgga
    12361 tttgacagga ggttcaaaag gatgatgaat cgatcgattg gtaaaaaagc caatgattat
    12421 ttcttagaaa tcattgaatg gattccacgt cttatccatt tacagctact atggttcctg
    12481 tgcgtcttgc ctgttttcac attaggaact gcaagtcgaa cagttctgta tatgatttat
    12541 cattacaaaa aaaatgaaga gaaaaagctc ggttcactat tctggaaaaa gtttcgtgag
    12601 aacttctctc tttatggaaa gcaagatagt ttagcaagtg tttattttct tcttctattg
    12661 atcgactatc agattttccg ctattggggt gggggaattg gctatacatt gatgtatact
    12721 acgttgtttt tgcttctatt aagcgcgctt ctctttagtt acaaaatctt gcttcaactg
    12781 gaacaaaaag aagcttcttg gattactgca tttttcttgt tttttaatga tttcaaaagc
    12841 gcgcttttct atttggcagg cacgttgatc cttcttgtcg cattctggtt tgcaggacca
    12901 atctattttg ctttattagg tttcagtttc ttgttttatt ttcaagtcct gctttttatc
    12961 catcgtacgc aagaaaaggt tcttaaaaag gaggaataac atgttgcgtt tgattgctaa
    13021 aatcgatgat agtattatca acaagttcgc caagcaacgg aaaacggata gtcgaaaaat
    13081 caattttttg aaatttatca gtaatatctc taatggtggt tttatttggt tgctccttgc
    13141 tgctgtttta gtcttgatac ctgatactag agaatatggt ctagccattc ttatcggcag
    13201 tttgatctat ttttttgttg gtttgctttt tatcaaaaaa acgacaagac ggaaaagacc
    13261 ttatgaaaaa aatcgtcggc ttgtccttta cacaagtaaa ccgaagactt cttcttttcc
    13321 ttcaggacat acagcgctag cctttttatc agtagctgcg ttgttcgaat taagtccagt
    13381 tgcaggaata ttagcctttt tatttgcttg tttgatcgct tattcgagga tttttttact
    13441 tttacattat cctactgaca ttttaggagg agctatttta ggaagtgtta ttggcatcct
    13501 atgcgctgcc gttatctgaa ttaattgtac gaataaagca tgagtgcgta aatgcgcatt
    13561 tgtgctttat ttttttgttt cgaggaaagc gtagttgttt attcatttgt cttaataact
    13621 taagttttgt taaatgagaa cgttataaaa ttttataatt taattt
//
LOCUS       DACEUF010000070        13540 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.073, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000070 DACEUF010000000
VERSION     DACEUF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.073"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(63..1010)
                     /locus_tag="IXG20_002022"
     CDS             complement(63..1010)
                     /locus_tag="IXG20_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ1610211.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1200..1469
                     /gene="rpsN"
                     /locus_tag="IXG20_002023"
     CDS             1200..1469
                     /gene="rpsN"
                     /locus_tag="IXG20_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1610212.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1645..2007)
                     /locus_tag="IXG20_002024"
     CDS             complement(1645..2007)
                     /locus_tag="IXG20_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1610213.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2018..3139)
                     /locus_tag="IXG20_002025"
     CDS             complement(2018..3139)
                     /locus_tag="IXG20_002025"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ1610214.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYIHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3155..3505)
                     /locus_tag="IXG20_002026"
     CDS             complement(3155..3505)
                     /locus_tag="IXG20_002026"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ1610215.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGKVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3635..5146)
                     /locus_tag="IXG20_002027"
     CDS             complement(3635..5146)
                     /locus_tag="IXG20_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1610216.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5468..6820)
                     /locus_tag="IXG20_002028"
     CDS             complement(5468..6820)
                     /locus_tag="IXG20_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ1610217.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAVKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6973..8049)
                     /locus_tag="IXG20_002029"
     CDS             complement(6973..8049)
                     /locus_tag="IXG20_002029"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ1610218.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8245..9990
                     /locus_tag="IXG20_002030"
     CDS             8245..9990
                     /locus_tag="IXG20_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ1610219.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLKKEEQTQASRKTEIVHEKEFGSFAFRASTSTEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10239..10706)
                     /locus_tag="IXG20_002031"
     CDS             complement(10239..10706)
                     /locus_tag="IXG20_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ1610220.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFCQALKKAIDQGLPSTEDSR"
     gene            complement(10690..12063)
                     /locus_tag="IXG20_002032"
     CDS             complement(10690..12063)
                     /locus_tag="IXG20_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610221.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGIMYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12384..12872)
                     /locus_tag="IXG20_002033"
     CDS             complement(12384..12872)
                     /locus_tag="IXG20_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1610222.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12894..13496)
                     /locus_tag="IXG20_002034"
     CDS             complement(12894..13496)
                     /locus_tag="IXG20_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1610223.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3864 a         2938 c         2458 g         4280 t
ORIGIN      
        1 attttttgtc tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt
       61 tcttattttt cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga
      121 tgtgcttgga tgacatgctt aagggaagca gacagattca ttgaaataaa aacatgaaag
      181 gctccttttt ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt
      241 cttggaacaa acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg
      301 tcatttaatg caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg
      361 atacttcctt tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga
      421 tttgttttat cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt
      481 ttactggatg cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt
      541 gacccgaccg cacccgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc
      601 aaatggttga ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc
      661 caactcattt tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg
      721 taagtcccgc cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct
      781 gaaccaatct ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat
      841 gttcgtatct ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat
      901 gcttttattt ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct
      961 tcaaaaacat cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc
     1021 tccctttttc tttaagttta cgtttatgcc tttattttga caaaaaaaat gtcttttcgt
     1081 tttggcgcat gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt
     1141 atctaaaagc gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga
     1201 tggctaaaaa atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg
     1261 ctgctttacg ccaggaatta aaagcgcaaa aagattacga aggcttgcgc aaacttcctt
     1321 tagatgctcg tccagaacga ttgaaaaatc gtgatttgat cgatggacgc cctcgcggat
     1381 acatgcgtaa attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga
     1441 ttccaggagt aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg
     1501 gcgcatggac cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg
     1561 ctggtactag aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa
     1621 ggctgcatgg ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac
     1681 actaattttc aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt
     1741 ttctttcaat cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct
     1801 ttctattcct gttccagtag ctttgatccc aatatgagtc ggaagcttag gtttagccat
     1861 ttttgccgtt atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg
     1921 aataaccaaa actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc
     1981 aaaataaata tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta
     2041 tccgctgtcc taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat
     2101 cctgcatagt gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag
     2161 gaaattcttg aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac
     2221 agtagttccc ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat
     2281 aaccgatcgg tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc
     2341 cgattccttc acctgctggg agttttttga cttgaattaa ttcagaaacc agctcaagtg
     2401 ctggctgcag tggataaact tccggtagtg cgtgcccaga gggattcagt ccgtacattg
     2461 ctactccata acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat
     2521 tgctgacatg tatatatcga ggaagttcgg gaagtttttt tagcacttct ataaagcgtt
     2581 ctttttgcag attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata
     2641 aaccttccca ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt
     2701 caggtgtaca aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag
     2761 gcgtctctag cttacttttc tctaaaacag cagctgctgc ctgcaaccat tcccgtgtac
     2821 ctgcagttac tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat
     2881 tcaagataag gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat
     2941 ttgaaacaca aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc
     3001 catgaccgta tccgtttgct tttactactg caaacaattc ttttccttgc ggaagtcttg
     3061 caacttcatt tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc
     3121 ggtgccaagc gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa
     3181 aagctgtttc atttgtatga gtaatactga cccatacttt gccttcatga ggtgaacgcg
     3241 tcacaacagg ctgaccgttt tcatttttta aaatctcgac atcttgaaag gtaacttttc
     3301 cgatcccagt tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa
     3361 attctacttg gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga
     3421 ttcgtgcaat gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg
     3481 catcgatccc aattcctttt atcatatgct tttctctttt caataaattt agaacactct
     3541 cattctacca aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag
     3601 tttctcttaa aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa
     3661 atccgcgttt tttatcgttg tgacgtttac cgccatcttt ttggctgtta ttatttttgc
     3721 tataaccgcc ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt
     3781 ttccgccacc acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt
     3841 ctggtgtgat cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa
     3901 gtgcaactaa atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtact
     3961 tatctagacc attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac
     4021 ctttgaatgc ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt
     4081 tttcaatcac atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc
     4141 cttttccggc acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga
     4201 tatcatagtt gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg
     4261 tcgctacaag gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt
     4321 gttgagacaa atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg
     4381 ccaattcgtc tacacggcgt tttgtacgtc cgaagacgat tgttagttca ggtgtttgaa
     4441 catcaaacaa gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt
     4501 gatcgatcaa gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca
     4561 tgaatttaac gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt
     4621 gtctttgatc aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca
     4681 acatttcatc tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc
     4741 ggttgatgtg gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca
     4801 agccacggat ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt
     4861 tgtcgcgccc taaacgatat aattcttctt gtgtttggat agctaactca cgtgttggtg
     4921 cgataactaa accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc
     4981 caaatgcagc tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta
     5041 atgctagtgg aatcgtttct gattggatag gtgttgcttc ttcaaaacca gcgcgttcga
     5101 ctgatttcaa caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat
     5161 atggttgtta actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg
     5221 tattgtttct ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac
     5281 caccgaaaaa tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc
     5341 tttatcatta ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat
     5401 ttctcttcat ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta
     5461 cagattttta tttcatttgc tgaagctgtt ctaccacttc aaataagccc atgctgttgc
     5521 tgccttttag cataatacta tctgttgctt caagattttg ttttacagca tcaatcaatt
     5581 tattcttttc tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt
     5641 cttttagtgc atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga
     5701 tatgttcact catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg
     5761 ctaacacagc gattctgcgt ccttttgttg gtagattttt aaaagtatct aataccagtc
     5821 ccatggctgt tggatttgca ttatagacat cgctcaaaat agctgcacca tttcctgccg
     5881 ttagccattc ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag
     5941 gtatggacag tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat
     6001 aacttccagg cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt
     6061 ttccttttgc tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg
     6121 tttgtactaa tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta
     6181 atccgtctgg tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg
     6241 acccaagatt ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag
     6301 ccataagtga aagttccgtg atttcattgg cgtgatccat gcccatttcc aagacgatct
     6361 tttctgtatc ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat
     6421 ttccttgcgt cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg
     6481 tggtggtttt accattgctt cctgtaattg caacgacttc tgcacctact ttttccaagt
     6541 aataggttgc caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat
     6601 cttttggagc ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg
     6661 cttgctggat aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt
     6721 cggttatctt ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc
     6781 tgcccacggc ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag
     6841 gttgtgaaaa aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa
     6901 tttctctatt cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcttctt
     6961 ctttatttct ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat
     7021 tcctaattga atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa
     7081 gagtgggtac atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa
     7141 ttcgttttta tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt
     7201 gtaagctaac ttcgcgtact cttgcgcttt ttgataaact tcttccggca cttcggctgg
     7261 aatctgcatt tcgattttat tattgatata tttggcttca taatcataga atgctacgtc
     7321 ttttacgact tcgccaggca aagtcgtccg aacatcttca tttcctaata cagcaacttc
     7381 gatttcgcgc gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt
     7441 tgctaaagca ttttgcagct cttctcggtt ttctgccttt gtaatgccga cactagaacc
     7501 catattcgca ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac
     7561 ttttttagga ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac
     7621 accagcagct tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa
     7681 tacgcctgcg ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc
     7741 ttccccgttt ggcccgtgta aaactggaaa aacgatggct ccttcttctt tgatttctcc
     7801 cggattgatc acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag
     7861 atgcaagaca tctttgctag caggtttttc tgttagtaat ggacctttga cccattgtcc
     7921 ttcttttgta ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac
     7981 tgaaaatgcg gaaagaatgg aaacttcatg ctctgcgctg cgtccgccat atagtaaagt
     8041 aatcttcaaa agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt
     8101 agcacaaaac aaaaataaac gaataactta tttataaaaa aacgagttgg tttctaaaag
     8161 aacaaacgtc atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta
     8221 tgataaagaa aaggagggat gatgatggcg caaacaaatc cacgtagccg cttggcagaa
     8281 atcattcgag tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc
     8341 gaacaggtaa aaaaagcctt tgaagaacta ggccccacct tcattaagat aggacagatg
     8401 ctttctgttc gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa
     8461 gatagtgtac ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct
     8521 ctttcattat cagatatatt cgatgatttt tccaaatcgc cttttgcctc tgcttctatg
     8581 gggcaggctc atcgcgccaa gctgaagaac ggtgactcgg tcgtcgttaa gatccaacac
     8641 cctaacatcg ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg
     8701 atcaaataca ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa
     8761 cgttccttga tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat
     8821 caaaagaaca atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg
     8881 aaaaaagtga tcgtgatgga agaaatgtct ggtaaaaact tgaaccattt aatgaacgcc
     8941 gaaaacaaaa ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt
     9001 gcaaagcttt tagttgagaa ctttatgaaa caggtatttg atgatggttt tttccatgct
     9061 gatcctcatc cagggaatct gctattccat gtactaaaaa aagaagaaca gacacaagct
     9121 agccgaaaaa ctgaaatagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc
     9181 acttctacag aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg
     9241 gggcatcttt ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg
     9301 aaagatgctt atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt
     9361 gatgaaagtc ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct
     9421 attgatgaaa tcaacttgca agaagtattc acacacgtcg tgacgatttg tcatcaaaat
     9481 aatctgcagt tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg
     9541 gtgatccgcg tccttgaccc tgaagtttcc ctcatggaag tcgcttcgcc ttttgcccaa
     9601 cattattttc tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta
     9661 ttggaaggga tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg
     9721 tggacttcgg gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc
     9781 aggatcgaaa gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta
     9841 gggtcctccc tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta
     9901 gggatattta cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg
     9961 atccaaatgt ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc
    10021 aagaaataag aagtgatttt gcataaattg tttctcaaat ttttgtgaaa attcggctta
    10081 tttttcgaag aggctacttc tgaagcaccg agaataaaaa atgtgaaaaa gctgttctgc
    10141 acccagtaat aagaacaatc aaaaaggaac atggagcaca tttatcctgt ttccctgttc
    10201 cctttttgat gtattcgagc ataaataaaa taccttcgtt acctactatc ttctgtactt
    10261 ggtaaacctt ggtcaattgc cttttttaac gcttgacaaa aagacatatg atctttctcc
    10321 gtaaagggtt ttggaccctt tgtccttatg ccagcttttc ttaactttcc actatgataa
    10381 cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga gtgatggaac
    10441 actcggacgc ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt gatcaaaaag
    10501 tcattcttct taaccatacc gacgatttta taatccgcac tatcagctcc gcggtctacg
    10561 tagacgattt ggacatgatt agcgtattct ttggtcgtat aatggtccac actggtcacg
    10621 ataaccacgt caagaccata ggattctcct aattttatga catcttcttt tactggagaa
    10681 ccatctccat caatgaatag cctcattctt tttcgccttc ttccataaat gataaacagt
    10741 tactaatccg ctcagtaaaa gaccaacatt gatccatttt gccaagataa gcagttgtac
    10801 actttctatc ggcactgatt catggttcag cgtgatcaat aatagatgga ttggctctcc
    10861 catttctttt gctaaaacaa acaaaagcgt catcaataaa aactggtgga tcacactccc
    10921 tttattttga gtagtatact tgacaacgat ccggcgatcc attggaatag ctgcatcagt
    10981 ttgataatat tccttattta gccagctgcc tgttactacg tgccagaaac taagcagaaa
    11041 gacacctatc gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac tcagtccaag
    11101 caattgcata gccaatggag accatgttaa acgtttcaaa atccatggtc cgactatttt
    11161 tgctgcaatc aagcctgctg cataaggcaa atacataaaa atcgaaagtt tttctaatcc
    11221 gtacattatg ccaacataaa aactgctaaa taaaaaaagg aaattcccta gcattccatt
    11281 tagaaaagta aacagattta gccagcttgg caacttccat tccttcttct ggtgtcctaa
    11341 aacaaacacg aacaagatga acagcagcaa gaaaccataa atagcaagat caaaaagcct
    11401 ttcatcgaat aacaatcggg catttctcaa aagaaacaat aagccaaaga atatcaaaaa
    11461 taaaacaagc tctcgcgtag ctaccagatt tcttttgatg tctttgaaat ccagttcgta
    11521 acgcggataa tgactaaccg tatggtaggc catgacgtaa aaaagtgtat aaatagtaaa
    11581 gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga gaaatagcca
    11641 gatagctgca aataagtact ttttcccatc catattgata aaaccttgcg tcttttcgtg
    11701 aaaattgacc gtggtattag caggtacaag ccatgctgca ctcagcccta gcaaaacact
    11761 tgaaaaataa aataaaggaa aataaaatac gcctaatatt ccaagcaatg aaccagcacc
    11821 accaatcagc agagcgatca tcagcaacgt gtaactatct agaccaaatc gtagactgtt
    11881 gactaaaaat agtccagtca tccgaaaggc ataaaacagc gtaaatggca aaatcgtcag
    11941 ccaattatgc gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa taaccgtgtt
    12001 acttaagata aagggcgttg tttgtgtaaa attacgccca aatcgtttga gtttttcgaa
    12061 catgacaatg gctccttaaa cagtcttata actaatctgc tcttcgattt tagcattaaa
    12121 ggggaaataa accaatttta cagaatatat tcttgtaaaa aaaatacaga aagaagcata
    12181 agcttctttc tgcattttta cggtacttga ttactcaagc agacaagccc ttctccacaa
    12241 aagtcttgcc cgacacccct gcatccggca ggtgataaag atagaaatca attatcctgt
    12301 gcctccaaaa tcaattagga gctctgtaaa ccagagtcct tctcgacccg tcgcataaat
    12361 ttactttaac acagttccta tgtttatgcg agagcttttt tcattttata atcgaccatt
    12421 tgaaatcctt gaccgatatc tgaaataagt tcttctgtaa tctcaaatcc ttcatgctta
    12481 taaacctcga ttgcttgatg attcccctga ttcactcgca aaaagatctc tctttccttt
    12541 gtttgcgcag caagttcttc ataccagcga aaaactgaag aagtcaatcc ttgaccacgt
    12601 actttattta gcaaatataa tttacttaaa taaatttttc catttatctc ttcataagca
    12661 gtgtaaccga cagtcttttc ttcgcttttt aataaaaagt aggacacacc ttctgtcagt
    12721 tctttttgaa tctgtttttt tgactgataa gttttcagca tgtaatccac ttgtttttga
    12781 ccaatgatcg gcacaaaagc ttccggccat acttcttgta tgactgccat cagctcatcc
    12841 agttcatttt ctgttacttt tattttttcc atacacccac gtcctttttt acattataaa
    12901 tgaccttgcg cttgatggaa taaaaagagc atgatcatgc tatacacacc caccgaccac
    12961 ggtttcaaga aaatcacaat ctgcataaat tccctaaaag ataatttcgt caaaccagct
    13021 actagacaga tcaagtcgtc tggcgcaaag ggaagcagca aggtaacaac tagtactttt
    13081 ttaatatctt tcgtttttct tgtcagcaat tcatcaaatt tctgatactt ttttggcgat
    13141 aaaataagct ggacaaaggc tacgccatag taacggacaa gcaaaaatcc gattgcttcc
    13201 ccaatcacta ggccaataca actatataaa agcccaaatc cattgccaaa catcagcatg
    13261 cccgctactg aggagatccc tcccggcaaa ataggaatga tcacttggat cacttgcaac
    13321 agaacaaata tcatcactgc atacttgcca aattgctgga taaacgcttg caaagacgtg
    13381 ctcgatcgaa aaatcccatg atgataagca taaagaatca acacaaagaa aaaaaacaga
    13441 ccaataatgg gcatgatctg aagacatttt tgtaaaactg atcctttttt agacatctat
    13501 attccccaca tcttttttca tttattaaaa ctgcctcctt
//
LOCUS       DACEUF010000071        13080 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.074, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000071 DACEUF010000000
VERSION     DACEUF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.074"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(79..151)
                     /locus_tag="IXG20_002035"
     tRNA            complement(79..151)
                     /locus_tag="IXG20_002035"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Lys,seq:ctt)
     gene            532..1101
                     /locus_tag="IXG20_002036"
     CDS             532..1101
                     /locus_tag="IXG20_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ1610224.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1088..1948
                     /locus_tag="IXG20_002037"
     CDS             1088..1948
                     /locus_tag="IXG20_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1610225.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2138..2842
                     /locus_tag="IXG20_002038"
     CDS             2138..2842
                     /locus_tag="IXG20_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1610226.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2847..4682
                     /gene="walK"
                     /locus_tag="IXG20_002039"
     CDS             2847..4682
                     /gene="walK"
                     /locus_tag="IXG20_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ1610227.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4679..5995
                     /locus_tag="IXG20_002040"
     CDS             4679..5995
                     /locus_tag="IXG20_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610228.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5996..6865
                     /locus_tag="IXG20_002041"
     CDS             5996..6865
                     /locus_tag="IXG20_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610229.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6920..7729
                     /locus_tag="IXG20_002042"
     CDS             6920..7729
                     /locus_tag="IXG20_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1610230.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7832..8461)
                     /locus_tag="IXG20_002043"
     CDS             complement(7832..8461)
                     /locus_tag="IXG20_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ1610231.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8631..9923
                     /locus_tag="IXG20_002044"
     CDS             8631..9923
                     /locus_tag="IXG20_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1610232.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9999..10661)
                     /locus_tag="IXG20_002045"
     CDS             complement(9999..10661)
                     /locus_tag="IXG20_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1610233.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10886..11170
                     /locus_tag="IXG20_002046"
     CDS             10886..11170
                     /locus_tag="IXG20_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ1610234.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11221..12846
                     /gene="groL"
                     /locus_tag="IXG20_002047"
     CDS             11221..12846
                     /gene="groL"
                     /locus_tag="IXG20_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ1610235.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4341 a         2261 c         2829 g         3649 t
ORIGIN      
        1 aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat ttacctgttc
       61 cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc tgattaagag
      121 tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata atagtagcac
      181 taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt tacttcctta
      241 taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat accaacaaac
      301 aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc tttcccgccg
      361 tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag ccttcttgtt
      421 ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta gattgaatgt
      481 ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat gatggaaaaa
      541 atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct gcctcactgg
      601 gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt tgatcgttat
      661 ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat ggtaaacaat
      721 tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa agaacacgta
      781 gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga aatcaaagaa
      841 gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct attttgcgat
      901 gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc tcatccgcaa
      961 aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac tttatcgttt
     1021 gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt aaaagaggag
     1081 aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg atgtgccaga
     1141 agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt ataaagatca
     1201 ggtatataca gataaaattg acattacctc tgaagagatc tatcaacgtt tgccagagga
     1261 aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg agcaaatcaa
     1321 aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga gcgggacata
     1381 taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg tactagatac
     1441 gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat ggctggaaga
     1501 aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa gaacaaaagt
     1561 tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg gtttagttgc
     1621 ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg aagaagggat
     1681 ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa cggttgctta
     1741 cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc atggggatgc
     1801 gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac aagcaaacca
     1861 aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg gattattggg
     1921 tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag gtgaaaatca
     1981 gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg cactttcatt
     2041 ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata cggtataatg
     2101 agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt tagttgtcga
     2161 tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag gctatgatgt
     2221 ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag aacctgattt
     2281 gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc gtgaggtacg
     2341 caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa ttgataaagt
     2401 cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca accgagaatt
     2461 agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg aaccagaaga
     2521 ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag cttacatggt
     2581 gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac tcttttactt
     2641 ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg tttggggtta
     2701 tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc gtgaaaagat
     2761 cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg gttactattt
     2821 gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt cgatcagtca
     2881 attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa attattggtg
     2941 cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa gatatgaatc
     3001 aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa gataatgcag
     3061 atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg actagcgata
     3121 tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc gatgtcaatg
     3181 aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc acgactaaac
     3241 gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa ccgatcctgt
     3301 ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc gaacaaaaat
     3361 attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt gcggcattta
     3421 tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc ggtgaaatga
     3481 gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa gtgcatggac
     3541 aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt attgaagaag
     3601 ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc catatgacag
     3661 acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat atggctgttt
     3721 ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca cttttagata
     3781 tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg gtacttgacc
     3841 gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt tctatgatcc
     3901 gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg acagaacaag
     3961 aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa cttcgtacac
     4021 cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg aaagacgagg
     4081 aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg attcgaatga
     4141 tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt caattagaat
     4201 atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg atggtgggaa
     4261 atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt tgggtcgaag
     4321 tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg atcaagtatt
     4381 caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac gtggttttaa
     4441 gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg ttcgagcggt
     4501 tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt ggattagcta
     4561 tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc aaggaaggaa
     4621 aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa tggtgggaat
     4681 gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt taagcatata
     4741 cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg tagatgagac
     4801 aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct ttcttccgct
     4861 tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa atctcgtgta
     4921 ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag tagtttcagg
     4981 agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt tggcctataa
     5041 tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag atgtgtcgaa
     5101 cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc aaggaaataa
     5161 aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata tttcgttgaa
     5221 ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag aaatgacaac
     5281 agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac tgaaaaagta
     5341 tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt tccagaaacc
     5401 agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg ggaatgaaac
     5461 aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc cagagaaaaa
     5521 agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag gcagttcttt
     5581 agggaacctc agatattttg accgcagtga caatacgatc gattatcgga tattcgtgga
     5641 aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag ttggcggtga
     5701 aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc aagtgccgat
     5761 accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc agttaaaagc
     5821 taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat ggcaaaatgt
     5881 caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat ataaagataa
     5941 ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga attaattgga
     6001 tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg tgtttttatt
     6061 cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta atcagactga
     6121 atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga tttcgtctga
     6181 gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt cattagcgaa
     6241 ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag acggtgatgt
     6301 tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc cagataaaat
     6361 tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg gtaatgatta
     6421 tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc aagcaagcca
     6481 agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt ctttagaaaa
     6541 aaacgataat gattatagca tcacgaaata catgcagacg catttatctg atatcgagca
     6601 gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat tgtacatcaa
     6661 taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt cctgtattct
     6721 gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg aaacaagcga
     6781 taaagtggtc caaattgaga gtgtcaacgc tttcagcaat acgatcgtga caaacaatac
     6841 tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt tacaaagtaa
     6901 gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca gtatcctagc
     6961 aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc ttttggtcga
     7021 tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc ggacaccgga
     7081 ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg gcgtgggtgt
     7141 cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg aagcgatgga
     7201 accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg ggaaagtcag
     7261 aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag cggctccgca
     7321 attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca ccggatattg
     7381 cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg aaagtaatca
     7441 tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc gtattttagg
     7501 agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg tgatcggtaa
     7561 tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga aagaacttgc
     7621 tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac atgattttga
     7681 aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa cagttcaatc
     7741 aataaaaata taaaaagcag aagataaaat caatcagggt tggttttatc ttctattttt
     7801 atttttttag catattaaaa aaataaccgt attatcgact gactacactg atgtgctgct
     7861 tttcatgttt ctcatccagt atctcatcta ctaatgcaat cgcgtaatct gccatgctga
     7921 tcgtactgtc gccagcagcg tttctttgta aataatcatc acttaacaaa taagtacctg
     7981 tacgtttgcc atgtggatca aataaagaag ctggactgat ataagtccaa tgtagatttt
     8041 tagattgttt caattttaac aatccttcgt acatattgaa agctgttgga tagaagggtg
     8101 catttttagg tacttggtca atcatacgag tcgtcttttc tgtatctaag aataacgaag
     8161 aggcaccccc aacgacaatt aagcgtgtat cggttccttc aagcatctca atcaaatggg
     8221 acaaagaagt ttgatgcatt tcttcttttc ctttgggagc gttgaaagca tcaacgacaa
     8281 catcaaaaga tgcaagatca gtagttgtca actcaaataa atccttttgt ataaacggaa
     8341 catctgatgg caatttttct ggatgacgca cgattgccgt cacgtctaaa tctctggaca
     8401 atgcttcgtc taaaataaat gaacctgcat ggccagtagc tccaatgagt gcaattttca
     8461 taaaaaacct ccttttttat tcctgtccat tgtaagcagt tgctgaatat tcgtcaaaga
     8521 atccgatttt tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca ttgggtatag
     8581 ttaaattaag aaaactgatt gataaaaata gaagttgaag gagataagaa atgaaagagt
     8641 ggttagatcg tgccttttta ccaatgataa catctgttgt gaatcttaga cccttaactg
     8701 ctttgcgtaa tggaatgact tacacgatcc caataattat tgttggctcg ctgtttttct
     8761 tatttgctca ttttcctgtg gattacatcg ctcagtggat agacaaaaca ggtttttcta
     8821 tgtattttac acaagtatac caggctacat ttgatgtgat agccttttgg atcgtttttg
     8881 ccattgcata cgcttatgca aaagaagaag acatcgatcg cttaccagtc ggcataacag
     8941 ccttgtctgc ttatctactg cttattcagc cactaaatac tcagatagat actgtctgga
     9001 caggtgctga agggatacta ggagcagtta caactgggct catcatcgga tggggctatg
     9061 cacgtttagt aagacttgcg aaagggtgga agcaaagaga gaagatacct gaaaatgttt
     9121 ggcagtcttt tacttcattg attccaatca ttgttatagt cacttttacg cttatcggtt
     9181 cagcggtttt tcaacagttg ttcagaatga ctgttgttca aggagtatgg acatatgtcc
     9241 agcttcccat tcaatcgttc atagacacgt tgtttggtgt gcttttatta ggtttttttg
     9301 ttccgttttt atggttattt ggtcttcatg gatctgcgat ggtcaatggg ttggttagtc
     9361 ccgtcttgca agcgaattca ttagcgaatg cagaaatctt agcctcagga caagagttga
     9421 cggtggcaaa tggcggacat atcgttactc agcaatttct cgatcagttc atgacggtta
     9481 cgggtgctgg attgacgtta ggggctgtat tttttatgac ggtttttgcg aaatcgaaga
     9541 aatacagaga attaggaaat cttgctcttt taccagcctt tttcaacatc aatgaatcaa
     9601 ttattttttc cacaccgatc gtgatgaacc cgatgatggc tgttcctttt attttcgcac
     9661 cggtcgtttc aggattgatt acctattccg ctttatattt tggattcgtt cctttattta
     9721 cagcagtcca agtcccttgg acaacaccgc cgatcctctc gggtttctta gtaggcggag
     9781 tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag ttttttcatt tatttcccat
     9841 ttttgaaaaa aatagattct gcaaactgca aaaaggaaag acagacaaaa aatgatggag
     9901 cagcagaaaa gatgaacgac taaaaaacaa aaatactcct gtcgctttag attgggaaaa
     9961 tcaccaatct aggatgacag gagtatttat agtcatgttt atactagccc tttttggaca
    10021 gctaattgaa caagaacaac taatgtgttc attcctacgt gtgtgatcat cgatgtccag
    10081 atccgaccag ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa gtagattagc
    10141 agatgaccat cattatgagc aaacgcaaac agcaaagagc tgatcactgc tccgacccaa
    10201 aaatttgaat atcgactgat gatccccagt atactgcggc ggaatacgaa ctcttccatg
    10261 atgggtccag caacagttgt cgccaaaata aaagcgggtg cttccatgat catctggatg
    10321 atattttgtg tattctcgga aggggttgct ttgccaaaca atacagattc taacatgaca
    10381 gcgatgcttt gcagaatcaa tgcgacaaag attccaacca atccataaat aacaatcttt
    10441 ggagcagaag ctttttttac cgctgattcg atcgctaagg gttcacgttg cttaaaataa
    10501 aaaatagcta ggacaacggc gcctagtata tacatgactg cagttgctgt gatcaattca
    10561 gacgtagatt ttacaagtcc tatcgaagct aggatcaagg gtgagataaa aattagacca
    10621 tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca tgtaattcct ccttttaagg
    10681 gataacaagc atgcaaggca agcttatatc gattgatttc aattatatca taatttcaat
    10741 aagcagctgt cagaataaaa tttcttttct ttgatttaaa agcttgcaaa aaagagggag
    10801 aatgagtatt attaaaaatg taggttagca ctcgaaggtt aagagtgcta acagattaaa
    10861 aaaagagtta tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg tcattatcga
    10921 agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg ctgctaaaga
    10981 gaaaccccaa actgggacag ttgttgcagt cggtgaaggc cgtttgttag aaaacggtga
    11041 aaaagttcct gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat acgccggtac
    11101 agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca ggaaaagata tcatggcgat
    11161 tgttgagtaa atttggaaga taaaaaaata atcaaaaata atcaatgagg tgaatcaatc
    11221 atggcaaaag aattgaaatt tgcagaagac gcacgcgcag ctatgttacg cggggtagat
    11281 aaattagcag atacagtgaa agtaacatta ggacctaaag gccgaaatgt cgtactagaa
    11341 aaatcttacg gctctccatt gatcacaaat gatggtgtga cgatcgcaaa agaaatcgaa
    11401 ttagaagatc attttgaaaa catgggtgca aaacttgtat cagaagtagc atcaaaaaca
    11461 aatgatattg caggagacgg tacaacgaca gcaactgttc tgacacaagc aatcgttcgc
    11521 gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg cgggatcgaa
    11581 ttggcaacaa aagcagcagt cgaagaattg cataatatct caactgttgt tgattcaaaa
    11641 gaagccattg cacaagtagc agctgtttct tcgggttctg ataaagttgg acatttgatt
    11701 gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga atcaaaaggg
    11761 atcgaaacag aactagatgt tgtagaaggt atgcagtttg atcgtggtta cttatcacaa
    11821 tatatggtaa cagataacga caaaatggaa gctgttctag aaaacccata tatcttgatc
    11881 acagacaaaa aaatctctaa catccaagat attctgccat tgttagaaca aatcttgcaa
    11941 caatcacgtc cattgttgat tattgctgac gatgtagatg gtgaagcatt gccaactctt
    12001 gtattgaaca aaatccgtgg aacattcaac gtagtagctg taaaagctcc tggtttcggc
    12061 gatcgtcgta aagcaatgct tgaagatatc gctattttga caggtggtac agtaattact
    12121 gacgatctag gtttagaatt gaaagatgta acaatcgaaa acttaggaaa tgcttctaaa
    12181 gttgttgtag ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa agaagcaatc
    12241 gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa caacttctga ttttgaccgc
    12301 gaaaaattac aagaacgctt agctaaatta gcaggtggtg tagctgttgt taaagttggt
    12361 gcagctactg aaacagaatt gaaagaatta aaattacgca ttgaagatgc attgaatgct
    12421 acacgtgccg cagtagaaga aggaatggtc tcaggtggtg gtacagctct agtgaacgtt
    12481 atcagcaaag tttctgctgt agaagcagaa ggagacgtgg caacaggtat caagatcgtt
    12541 gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa acgctggtta tgaaggatca
    12601 gtgattgttg ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc tgctactgga
    12661 gaatgggtaa acatggttga agcaggtatc gtagacccaa ctaaagtaac acgttctgcc
    12721 ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga cagaagcagt cgttgcagat
    12781 aaaccagaac cagcagcgcc agcagctcct gcaatggatc catcaatgat gggcggtatg
    12841 atgtaagaaa aagcaacttg cttatctaca actgatcatt agtgaaagac agataaaaag
    12901 aggctggaac agaagcgttt aactccaaga aataagaaga aattcacgga aattgctttt
    12961 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gctgtttata
    13021 cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctct tttttgtgtg
//
LOCUS       DACEUF010000072        12859 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.075, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000072 DACEUF010000000
VERSION     DACEUF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.075"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            6..2378
                     /locus_tag="IXG20_002048"
     CDS             6..2378
                     /locus_tag="IXG20_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610236.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNDQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQVPEKEQKQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGHTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQD
                     EKNVVETAEQKNIVLVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDTQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2439..3899
                     /locus_tag="IXG20_002049"
     CDS             2439..3899
                     /locus_tag="IXG20_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1610237.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVQDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEMKVEPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKGCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGKQQERE"
     gene            3910..5919
                     /gene="topB"
                     /locus_tag="IXG20_002050"
                     /pseudo
     CDS             3910..5919
                     /gene="topB"
                     /locus_tag="IXG20_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            6265..6861
                     /locus_tag="IXG20_002051"
     CDS             6265..6861
                     /locus_tag="IXG20_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1610238.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6874..7773
                     /locus_tag="IXG20_002052"
     CDS             6874..7773
                     /locus_tag="IXG20_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1610239.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEDIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKEQKLAYQRHQQMNREMER"
     gene            7776..8260
                     /gene="ssb"
                     /locus_tag="IXG20_002053"
                     /pseudo
     CDS             7776..8260
                     /gene="ssb"
                     /locus_tag="IXG20_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            8282..8539
                     /locus_tag="IXG20_002054"
     CDS             8282..8539
                     /locus_tag="IXG20_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610240.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8698..8832)
                     /locus_tag="IXG20_002055"
     CDS             complement(8698..8832)
                     /locus_tag="IXG20_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ1610241.1"
                     /translation="MLKSIKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK
                     "
     gene            8975..9235
                     /locus_tag="IXG20_002056"
     CDS             8975..9235
                     /locus_tag="IXG20_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610242.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            9684..9890
                     /locus_tag="IXG20_002057"
     CDS             9684..9890
                     /locus_tag="IXG20_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ1610243.1"
                     /translation="MRKKFDYLGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIEEKGKKR"
     gene            9890..10141
                     /locus_tag="IXG20_002058"
     CDS             9890..10141
                     /locus_tag="IXG20_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610244.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGCELHYQENLETYENEYWLTKLEN"
     gene            10157..10594
                     /locus_tag="IXG20_002059"
     CDS             10157..10594
                     /locus_tag="IXG20_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610245.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCFEIGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            11275..11670
                     /locus_tag="IXG20_002060"
     CDS             11275..11670
                     /locus_tag="IXG20_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610246.1"
                     /translation="MKKKQTKLSLADILTQLTATEDGVVEFERSEISVVDDRYFKMPY
                     FFDQAKVICLCGYDGVRDYFGIRITEEKVVWVNNHTELGALAFEGTVLDNISIVFEEE
                     SFTLECDKLTRYIDPKFYEDKNLAWELAL"
     gene            11652..11918
                     /locus_tag="IXG20_002061"
     CDS             11652..11918
                     /locus_tag="IXG20_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610247.1"
                     /translation="MGTGFIDRVLVQVKEERKITMKYNLLDHKRMWVEKLEEDLEQLE
                     LLGHNKTSTVYKEGLKRTKKAQKELATLESRVQKKEVDLTHTYL"
     gene            11928..12161
                     /locus_tag="IXG20_002062"
     CDS             11928..12161
                     /locus_tag="IXG20_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HAQ1610248.1"
                     /translation="MADKRKEKIQKLLALAADVNDEESVSALAKAQQLMMDYDLTEDE
                     IFNYHEQQRIEAVETHVIYRGRPQKWLYRLFRN"
     gene            12504..12686
                     /locus_tag="IXG20_002063"
     CDS             12504..12686
                     /locus_tag="IXG20_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ1610249.1"
                     /translation="MSVSTLGITVDAATYYGNGVYCNTQKCWVDWSRARSEIVDRGVK
                     AYVNGFTKVLGGVGGR"
     gene            12676..>12859
                     /locus_tag="IXG20_002064"
     CDS             12676..>12859
                     /locus_tag="IXG20_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610250.1"
                     /translation="MEDKKEKLFDLIANAYNSKSYNNVEVKEGFLQDAKKIREDKPYP
                     EIIISVYKHLSKLKAEE"
BASE COUNT         4756 a         2107 c         2701 g         3295 t
ORIGIN      
        1 cggacatggc agaagaaaaa gaaccactag acaatagtcg tttgggaaaa tccaaaagaa
       61 aattagtacg attgcaaaat gaattaaatg atcaaatcga aaagatgttt gaacaccaaa
      121 gaaagaccaa cggtcaacca atgaacgata agagaaacgg gcattcttgg tttaggcaac
      181 aagaacgaat cgaaaataaa gtccaaagtc tgagagaaga gatcaagcaa caagaaaaac
      241 aggtagaaaa acttgaaaga caagaagagc taaaggaaat gggttataac aagtacggtg
      301 gcctggatat gacgatcgaa aatattccaa gaatcaaaga agaaatcgaa cgctttgaaa
      361 aaggtgaatc gactttttca gctgccacga tccgaaaata ccaacgaaaa ttggaaacat
      421 tagaacagct gaaagaaaaa tcagagaaag gaaaagaaaa tattcttcct gaagtacaag
      481 cgatcattga cagcggacga gtgacgcaat ggaaaaagaa tccaactatc tattttctga
      541 aaggctatcg aaaagtcgct ttagaactca atgaaaacgg gggatttgag gaatctaaaa
      601 aataccaagc cacgtcggaa gaagaaaagg cagctgttcg tgaattactt gaagaagcta
      661 cacctactga ttctgtgaag gagaaagaaa ccgatcattt ctatcgagta gaattcaacg
      721 aaaatgatcc agcaaaaggg ttgaaaagtt atggtgggga gatcgtaaca ctagaattga
      781 tcgatcaact caaaacactg gataaccaat acgccgcaga agcaggctat tataaattct
      841 attttgaaga agtgaagaat ggagaagtag tagatcagat acgaatggat ctaggggatg
      901 gcgtcaatag taatcacccc atctatttag agttagctag attgtgcgaa caagtacctg
      961 agaaggaaca aaaacaaaca gtcaaaaatg aacctatttc aaaagtacaa aatgagatta
     1021 aagagacaaa aacaaaacaa aagggttatc aaaaacgaac ggcagaagag attaagcaag
     1081 aagtcaaatc actttcccaa caggcgcttg atgcggtgaa aaagcatact cattcacctg
     1141 aagacgtcaa agaacttttg aattttatgt ctcgttttcc agaacgatcc tttagaaatc
     1201 aattattgat cgaggaacaa tttccaggcg caacggcttg tttaggtcgt gccgcgttga
     1261 aaaaagaagg aatctatatc aaaaaagggg aacaagggaa caagatcttt gtcagaaaaa
     1321 cggtcaaagg attttatgag aaaggacgtg gctttgttcg agaaacagag gctacaccag
     1381 aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat caaaaaacct tactacacga
     1441 ttgaaaaagt gtttgatatc acacaaacac aattgaagcc tgaagactat ccgaaaatct
     1501 tcccgaaccg cgtctttgat tttcaattgg atcaaacagg gcatacagaa cttcaagttg
     1561 ggattcaagc ggtagccaaa aatattggaa ttcaaattcg agatatgaca gaaagtgaag
     1621 tctatcaaag agaattagga caagcacgtg gggcgtacgt tcgtaacgaa tttactggcg
     1681 aagaagaaat cgtcatgaat actcgaaata ctcctacgca gcatttagcc acaagtattc
     1741 acgaattagc ccatgctaga atgcacaaat tttcagaatt agatactgca acaaaagaac
     1801 tacaggcaga aatgacctcc tatattgtgt gcaagcattt tgggatggac acctcggaga
     1861 aagctattcc ttatattgcg acatggacga agaatggtca gacgttggat gataaggaag
     1921 ctgcggaacg tgggaaaatc atgaatgatg tcagtcgtgt cgcaaatgaa ttcattcaaa
     1981 ccatttctaa tgaaatcaat caatacagag aaagagaacc agagatccag ccagcagaag
     2041 tgaagcagga tgagaaaaat gttgtagaaa cagcagaaca aaagaatatt gtgctcgttg
     2101 gctcactttg gatcgacaag aaagacggga aagtcctgga agaagacacg ggccgaacgt
     2161 tacatttgaa ggatgcggcg tttattgaag atacgcagca taatcaagtg attttaaaag
     2221 gaaaaagtta tggaaaagag attcaaacgg cgttaccttc agaagaattt gcgctaggaa
     2281 atccattccg attttcactt gaaacagtgt cagaagtggc caaacgaaaa aatgaaatct
     2341 attatgaaca acaattgtca caaaaagaac gcgtataaaa caagtaaaaa agactatctg
     2401 aaaaacggat agtctttttt cttttgggag gtttgaaaat gaccgagaaa aaaatgagtc
     2461 ttattgatcg atgtaaacag atcgatatag tggattttgc tcgaaacaat ggaatggctg
     2521 tagtcaataa aggacgagac tatcgcttag aagaccatga ttcatttgtt tttgatcgaa
     2581 gaaaacaacg tttttattgg aacagtcaaa atattagtgg cgacattatt gaattagcta
     2641 aacttttttt catagacaaa gagattcaag actctaagca acaatttaaa gcagccacag
     2701 attttattct aaagaatgaa gacaaaacag aacgagtaga aaatcttcat tttgagacag
     2761 aaaagtacaa agatcacccc gttgattatc aaccactcac agaaaaagga aggaattatt
     2821 tgaaggaaga acgaaaactg cccgattggt tgattgatta tgcagaaaaa gaaggattga
     2881 tcgccgaact aaagccaaaa catgaacggc aaaatttctt agttcaagac gatcgtttgg
     2941 atcatgcggt cgccttttta tggaaagatc cacagacaaa agagacagta ggggcgagtt
     3001 atcaaggaac attcattgat tatgaacgtt ttggtgagag aggcacatat aagcatatag
     3061 ataaaaattc gacagcgaat catggattta acctaaagat cggtgaccct aaacagttaa
     3121 aattttttga gagtagtatt gatttactta gctatgctgc actgaatcgt gatcagttaa
     3181 acgatacgtg gttagtctct atggaagggt taaaacacca tgtgatcagt cattattttg
     3241 gtgaggcagt atctgaattg agaaagaaac aagcctttcc tcaatccatt gagatttgtg
     3301 tggataatga ccgtgcagga catattttct acgaaaaaga acaattaatg ggcgcagttg
     3361 atccatttac gaatcagaaa gtacgttgtg aacgaggaat cgctaatgat tggcaagtgc
     3421 caaaagaata caaagtcatt tatgaagaag ttgcaaaaga aatgaaagtt gaacctgaag
     3481 caattatggc catacacaag acagaaaaca atctgcaatt aacgaaccaa ctggtttccg
     3541 ctcacaaagt aaatgcgtct tttggtcagc agctttctgt taacgattca atagaagcta
     3601 ttaatttgaa agatatttgt agagaagtcg caaaagaatt aaaaggttgt gaacgcgtgg
     3661 atggtacgta cgattttgat cgtttttatc aagaaaaagg ggatattaat gctcagattc
     3721 ttttctctta taaagcagaa caatactaca aaggctataa aaatcatgaa catgaatttg
     3781 tacctgaagt aaaaaaggat tggaatgatc aattgaagca tgaaatccac caacaagaga
     3841 tcagaaagca aaaacgtgcg atgttgtttc aacaaggcaa acaacaagaa agggagtgaa
     3901 atcgtcatga tgaaaaccgt tattttagca gaaaagccgt cacaagcgaa agcttatgcc
     3961 gatagcttct ctaaagccac ccgaaaagac gggtattttg aaatccaaga tagattgttt
     4021 cctggtgaaa ctgtgatcac gtatggtttt ggtcatttag tcgagttaga ttcgcccgat
     4081 atgtacgatg aaaattggaa acaatggtca ttagagcatt tgcctatttt ccctacacat
     4141 tatcactatc atgttcctaa agataagaaa aagcaattca aggtggtgaa acagcaactt
     4201 caatcggccg acaccattat tattgcgaca gatagcgatc gtgaagggga actgatagct
     4261 tggacgatca ttcaacaagc aggcgccgat catggaaaga tatttaaaag actttggatc
     4321 aactccttag aaaaagaagc catttatcag tgattccagc aattacgaga cgcagaagag
     4381 acctatccca aatttgaaga agcacaagca cgccagatcg ctgattggtt gatcggcatg
     4441 aacggcagcc cactgtatag tttactcttg cagcaaaaag gcataccagg aagtttttcc
     4501 ttaggcagag tccaaacccc aacgctttac atgatttatc agctgcagga aaagattaga
     4561 aacttccaaa aagaaccata ttttgaagga aaagcccaag taatcgctca aaatggcgca
     4621 tttgatgcga agcttgatcc aaatgaaacc caagcgacac aagaagcatt tgaagcctat
     4681 ttaaaagaaa aaggtgttca attaggaaaa cagccaggta cgatccttca agtcgaaaca
     4741 gagaaaaaaa gtgcggctag cccacgtctt ttttctttat cgagccttca gtctaaaatg
     4801 aatcaactca tgaaagctag tgccaaagat actttggaag ccatgcaagg gttgtatgaa
     4861 ggaaaatatc taagctatcc gcgaacggat accccttata ttacggaagg ggaatatgcg
     4921 tatctgttgg atcacttaga cgaatacaag cattttttaa aagccgaagc gattccgact
     4981 cctattcata caccgaatag tcggtatgtg aataataaga aagtccagga acattacgcg
     5041 atcattccta caaaaacggt tatgacagca gcggcttttg aacaactaag tccactgcag
     5101 caggctattt atgaacaagt gttaaaaaca acggtggcca tgtttgcgga aaagtatacc
     5161 tatgaagaaa caacgatctt gacgcaagtt cagcagcttc agttaaaggc aatcgaaaag
     5221 gtgccgctag atctaggttg gaagaagtta tttggaaaag agagcgacgg aaaagagaag
     5281 gaagaagagc cgctacttcc taaagtgaca aaaggagaaa tggtgacggt ggatctacaa
     5341 gttcttgaaa aagaaaccaa gccaccacaa ccttataccg aaggtacgtt gatcactgca
     5401 atgaaaacag ctgggaaaac cgtggatagt gaagaagcgc aatccatatt gaaagaagtg
     5461 gaaggaatcg gtacagaagc gacacgggcg aatattattg aaaccttgaa acaaaaggaa
     5521 tatatcaagg tagagaaaaa taagctcgtg gtgacgaata aagggatttt gctttgccaa
     5581 gccgtggaaa aagagccgct gcttacgagc gccgaaatga ccgccaaatg ggaaagctac
     5641 ttgttaaaga tcggtgaacg aaaaggaaca caaaccactt ttctagccaa tatccaaaaa
     5701 tttgtctctc acttattgga agtcgtacca gggcaaatcc aatccactga ttttggatcg
     5761 acgttacaag aagtgaaagc agccagtgaa aagcaagaag ccgcgcgtca tttaggaatt
     5821 tgtccaaaat gccaggaaca agaagtctta ctctatcaaa aggtggcagc ttgtacgtct
     5881 gaagcctgtg attttaaaag gtcagaaggg ttcatttgaa gcaacgattg ttttaaaaga
     5941 agacttcact acaggctttg aattttctga aaagaaaaaa acgaacttca agaaaagaac
     6001 acgccgcact gcaaaataat ggagggttaa tcttatgcgt aataccacac acaaacaaca
     6061 agaaatcgaa aatcaaaaag ccaaaattcg ttcagaacag gaaaaaatgc gtgccagcaa
     6121 acgaagactg cagcaattga agaaccagca gaggattcaa aaagaaatcg aattagatcg
     6181 ataaaatctt cctaactatt gtatacgtat aaacttatgt tatactgttt ttataagttt
     6241 atacgtatac aaaggaggga gaaaatgaca aaagcaatgg tatacaagct gctagaagaa
     6301 tttaatggaa ctttggcttt gaaggatgcc aaaaaagcgg gcatttctcc tgtaacgatc
     6361 aaacgtatgg tagatcgagg ggagctggat cgagagtatc caggtttttt caccttacct
     6421 ggtcaatttc ctgatgaatt gtttatggcg caaaaaaaat atgaacgcgg cattatttca
     6481 catattaccg cgttagattt gtatgattta acagatatga tccctagaca gatcgatctg
     6541 actgtacctt acggttatca cgtttctgaa aaaggattga aggaattcgc agttgagcta
     6601 cactatacaa aagcggaatg gtatgaatta ggtaagattg aaataaaaag tcgctatggg
     6661 aatcctatta tagcttatga tccagaaaga actttatgtg acatttggaa tccctggtat
     6721 accgttgagg atgaaatcaa agtaaaagca atcaaaaact atatggagtc ggacagaaaa
     6781 aatcttagaa aattgaatga gtatcgacga attttaccaa cagacaaaac gatgcgttct
     6841 tatattatgg ctttaaatta aaggagaaga tacatgacac ctacgcagtt aaaagcaaaa
     6901 gtgaagaata tttctagaga aaaagaagtt gatccgcagc ttgttttaag acattttatg
     6961 atggaaaaat tcttggaaaa aatctcagag tctccttatc gtgaaaattt tgtgttaaaa
     7021 ggcggcttct tgattggctc aaagtatggc attgaaaatc gcacaaccaa agatattgat
     7081 acgactttac gggaaatgaa agtaaccaaa gaaacattaa caacggttct aaacgaaatt
     7141 ttttcaacgc cgacgaaaga agacattcaa tttgagattc aaggaatgaa agaaacgaga
     7201 gaagccgatt actatccagg atttagctta cgtgtgctgg cacatttgga aaacatgcgg
     7261 ccagatttta aggtagatgt gactacaggt gactcgatct atcctgcaac gatcactcac
     7321 agtcataaat tgatgtttga ggatcgaacg attcctttgg aatcctatcc tacagaacaa
     7381 attattgctg aaaaattaag tgcaaccttt gattttttaa cagataattc gagaggaaaa
     7441 gatttttatg atctctatac tattcctaaa atggagaaga tcgatgagaa gaccttgaaa
     7501 gatagtgtga gaaatacatt tacacgtcga ggaaaaactg atccgttaaa gatatactat
     7561 gaaaaggata tggatgtact gaaaaatagt cacgagctaa aagatacctg gaagaaatat
     7621 caaaaaagca atccttatgc tgcatcgatc acgtacgatg agacaatgga ttcgattagt
     7681 aatttgatgg aaaaagtgat tcacgtcgaa gaaaaagagc aaaaactagc ttatcaaaga
     7741 caccaacaga tgaatagaga aatggagaga taaagatgat taataatgtg acattagtag
     7801 gacgcttaac caaagatcct gatttacgtt ataccgcaag tggtactgca gtagcgactt
     7861 ttaccctagc cgtcaaccga aatttcacga atcaaaacgg caatcgagaa gccgatttta
     7921 ttaactgcgt gatttggcgc aaaccagcag aaacaatggc gacattagct aaaaaaggta
     7981 gcttgattgg tgtagttggg cggattcaaa cgcggactta cgacaaccag caaggacaac
     8041 gtgtatatgt gactgaagtt gtagcagata actttcaact attagaaaat aaagcagcga
     8101 cagagagccg tgcgcacgct gatcaatcgt ccaccagtcc aagcaccgcc acctttgaac
     8161 aacggataca gcgacgccta acaacaatgg attgaacgct tcacagaatc catttggtgg
     8221 acaatcaatc gatattagcg atgatgatct accgttttag tttgagaaag aaggagtaag
     8281 catggcaaaa aaaattagtc tgacgatgga tctattgata cccgtaattg catttataga
     8341 cgggatttct ttgggcgtca ctgaaaatcc tttggtaaat agaattgcga aaggatcact
     8401 atttcttgta ttcgtgtatc ttgtctgttc ttatctttta aaaagagcag gaaaggataa
     8461 ttccttttgg cgattgacta aagtcacagt agcagcgaca gctgttatac ttacggctat
     8521 tctaccgctt tatatgtaga gttaacggga aatcgttgaa cttcttgcga aatattgcta
     8581 taatgaacat gaaaaagagg agttgcgcta acaacccctc attaacagca ccgattaaag
     8641 aacggtggca gaatattagt tatttggaaa taaccgctga cctgctaaag tagacggtta
     8701 tttctttttg tcatttttaa gcatatttac aatcaaacta atcaaagcaa tggtaaacat
     8761 accaaaacca agaatggttt gaattgtctc atatgcggac aaaaggctta ctccttttat
     8821 agattttagc acttacattc ataagcacca ccacctttcc agaagtcgcc accgtcttca
     8881 aactttactg tcttgactat tatagcatat ctaaaatcga ataaaaattc tcaattttaa
     8941 ctgtttttgc tataatttta gcgaggtgat cgtgatgaac aaatatgttg gtttgcttga
     9001 taaaatccga gtaataaaaa cacaaccatt attggttcgc tttactttac aaacaattca
     9061 tgaatcgatt aattgtgtag ttgcagatat tgaaattatc gataaattgt taattatgga
     9121 tgatggaaaa tacaatattg ccgtcacagg tcatttcaat aaacggaatc aattagtcat
     9181 tgaatcgatg caaattagaa accctgatca ttttacgcga tcaatgggca tataaataaa
     9241 agaagagctg caatcctaat gtaggactgc agctcttttt gttttacata gaaacaggaa
     9301 gaaagggaaa ggcctgttta ggaaggcaca agtgccttct caattgaaaa acctttctct
     9361 cactccaacc gccaccgccg ttcgagaaga aaggaagtga acgttgcacg ttcactaaga
     9421 taaggggtct tgctttgtaa gacaaaatag gaaaagcttc ctctcaccaa aaggttcgtt
     9481 cctgttcgct atctctttat tgatttgcca gaccggcaac aagttgcctt ttatcaccaa
     9541 aaagcgacaa ggacaagggt aaatggcacg ctgcgcgccc ttgtccttgt cgcttttcgt
     9601 tgattcattc tggccaacaa atcaataaag aaagaactaa cgaaaggaag taaaaccttt
     9661 cctaagagga ggcagaatca atcatgcgaa agaaatttga ttatttgggt gttcccttca
     9721 gcgaattatt cccaaactat catgcaccac atactgtcga atgcgattgt ggagaaagag
     9781 cgaaatgtat caaaagctat tgtttgtatc aatgtccaac atgcgggaaa aaatatacct
     9841 tgtcttatgg cgattatatt ttgattgagg agaaaggaaa gaaacgctga tgaaaacaca
     9901 acacaaatta ttgactgtaa aagaagtcgt tcaaattttg gtggacaaag aagtcatttc
     9961 tgaaaaagta gcagaagctt tacttattcc gcggtactgg acgatagaaa cccctttagt
    10021 agaggatgca ccgactatgc tatttgaaga cctagtgaag attgcaggat gtgaactgca
    10081 ttatcaagaa aacttggaaa cttatgaaaa cgaatattgg ttaaccaaat tagagaacta
    10141 aaaaggagaa aaaaatatgg ctcaaaatta ctatgacgaa tttgtcaaat tacctttgga
    10201 taaaatggca caaaaaatgg aggatatgac gttcctatac aacgaaacac gtgttccaaa
    10261 aaaacattat aaagaaaaat tgtcggtagc tgtggaagaa atgattgaat caggtgtcga
    10321 aatgaatctt attgcgacgt actatcgaac actggaagaa ctgaaaaagc aaaatgcaaa
    10381 atggtttttc caagccttac tatgctttga aataggtgtc aaaccaagca ctattaaacc
    10441 ttctgaatac caggctttag aactaacgta tgcgaaattt atcgaaacga aaaaagccaa
    10501 gactgtttca agtgaatggt tagattattt tgaaaatatc aataagtatg gcgcatatta
    10561 caccatgaaa aaggaagaca acgaaaatga ataaggtgat ttatcaacta acaacggatt
    10621 ccatatcaac gatttgaaag aaaaaaaggt caataatcat ttataccgaa aagtgagaat
    10681 gtgtggaata aggacaaaaa aagcctagta actaagggaa tggttcttat ttcgcaccaa
    10741 ccgcccccgc cgcttaggtg gtgcgaaata aacgggcaaa gcccgataac ttaagagaaa
    10801 gaaggtgatc catttgaaaa agattattcc cttattgtta gtactgtttg ttggcgaaat
    10861 acttatgcga gatagtgcag ctgtacagtt aaacctcgct gtatttttgg taatactggt
    10921 gttttgtttg atcttttggc ggtttgtatt agtcctcgtg agtctactat tcaagaagat
    10981 tgcagccata tttttcatgg ggttatttcg atgaaaggag agagtaccaa tgccaattgc
    11041 aataggaaat aaacggttac ccgtgacatt agatgaaaag agacaaaaag aattgcagca
    11101 actaaagcag aagtacggca aaagtgaatc caggattatg tgtattgcgt tagatttatt
    11161 gattgccaag aaaaagcagg atttgaggta ccagcactaa gaaagtgacg tcacttttta
    11221 tactaaaacc taaaagtgat atcactttta gacaatggaa aaggtggaaa taagatgaag
    11281 aaaaaacaaa ctaaactatc gttagcagat attttaacac agttaaccgc aactgaagat
    11341 ggtgttgtag aatttgaacg atctgaaatt tcggtagtag atgatcgtta ctttaaaatg
    11401 ccttactttt ttgaccaagc aaaggtcatt tgcctttgtg gctacgatgg ggtacgtgat
    11461 tactttggaa ttagaattac tgaagaaaaa gtcgtttggg taaacaatca tacagaatta
    11521 ggtgcgctag cctttgaagg aactgtattg gacaatatct ctattgtatt cgaggaagaa
    11581 agctttacgc ttgaatgcga taagttaacc cgatatatcg atccaaaatt ctatgaggat
    11641 aaaaacctgg catgggaact ggctttatag acagagtgct agtgcaagta aaggaagaaa
    11701 ggaagatcac catgaaatac aatttattag accataagcg aatgtgggta gagaaattag
    11761 aggaagactt agaacagttg gaattgttag gtcataataa gacttcaacg gtgtataaag
    11821 aaggactaaa acgaacgaaa aaagcacaaa aagaactagc aacactggaa agtagggttc
    11881 agaaaaaaga agtagactta acacacactt acctttagga gtagaaaatg gcagacaaga
    11941 gaaaagaaaa gattcaaaaa ttactggcgc ttgcggcgga tgtaaatgat gaggaatcag
    12001 tgagcgcatt agccaaagcc cagcaattga tgatggacta tgatcttact gaagatgaga
    12061 ttttcaatta tcatgagcag caacggatag aagcagtaga aacgcacgtg atctatcgtg
    12121 gacgtccgca aaaatggctg tatcgtttat ttagaaatta agataaaaaa ggctagttaa
    12181 caggtttgtt gactggcctt ttttgatatg tttgtacatg ttttattgtg ttttaatttt
    12241 tctctttttt atagcaagct ccttttataa taaatattcc gaaaagtgaa gaccattttt
    12301 tatttattaa gactattatt attagtgaga taaaatttga ttgatcttac agaatattgg
    12361 tgacgattat gacactaaag atactattta tgaaaatttt gcaaaaatgt tctgaaaatt
    12421 acatataatt gttttataaa aaagggaggc aattatatga agaaaaaatt tgttagtatt
    12481 ttttgatttt aggaattgtt ttattgagtg tatctacttt aggaattaca gtagatgctg
    12541 caacttatta tggaaatggt gtttattgta atactcaaaa atgttgggta gattggagta
    12601 gagctcgctc tgagattgtt gatagaggag taaaagctta cgttaatggt tttacaaaag
    12661 tgctaggtgg agtcggtgga agataaaaaa gaaaaacttt ttgacttaat tgcgaatgca
    12721 tataattcaa agtcatataa taatgtagaa gtaaaagaag gatttcttca agatgctaag
    12781 aaaatacgag aggataaacc atatcctgaa attattatat cagtgtacaa acatttatct
    12841 aaattaaaag ctgaggaac
//
LOCUS       DACEUF010000073        12674 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.076, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000073 DACEUF010000000
VERSION     DACEUF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.076"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(147..1178)
                     /gene="queA"
                     /locus_tag="IXG20_002065"
     CDS             complement(147..1178)
                     /gene="queA"
                     /locus_tag="IXG20_002065"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ1610251.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKIGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGKMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1371..2546
                     /locus_tag="IXG20_002066"
     CDS             1371..2546
                     /locus_tag="IXG20_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1610252.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESDTPEESKEV"
     gene            2550..3191
                     /locus_tag="IXG20_002067"
     CDS             2550..3191
                     /locus_tag="IXG20_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1610253.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3188..4105
                     /locus_tag="IXG20_002068"
     CDS             3188..4105
                     /locus_tag="IXG20_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1610254.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVNNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4102..4773
                     /locus_tag="IXG20_002069"
     CDS             4102..4773
                     /locus_tag="IXG20_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1610255.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMVIVTDWLLGILERRLDPA
                     SRTSR"
     gene            complement(4969..5803)
                     /locus_tag="IXG20_002070"
                     /pseudo
     CDS             complement(4969..5803)
                     /locus_tag="IXG20_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619675.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6004..6270)
                     /locus_tag="IXG20_002071"
     CDS             complement(6004..6270)
                     /locus_tag="IXG20_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1610256.1"
                     /translation="MSNYTVAIKNSATVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6270..6533)
                     /locus_tag="IXG20_002072"
     CDS             complement(6270..6533)
                     /locus_tag="IXG20_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1610257.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDDIDWEAL"
     gene            7008..7193
                     /locus_tag="IXG20_002073"
     CDS             7008..7193
                     /locus_tag="IXG20_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1610258.1"
                     /translation="MALNYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7335..9158)
                     /locus_tag="IXG20_002074"
     CDS             complement(7335..9158)
                     /locus_tag="IXG20_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ1610259.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9755..12460)
                     /locus_tag="IXG20_002075"
     CDS             complement(9755..12460)
                     /locus_tag="IXG20_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ1610260.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         3651 a         2636 c         2389 g         3998 t
ORIGIN      
        1 ttcaaggtgt cttttctata tgttttggaa aataaaaaag atgctggtgt catccaccaa
       61 catcaaaaag tatagagctc tttttctttg tcataaaatc tttgtaaagc ttttttgtta
      121 ttcaaaacta gcggcttttc ataaccttat ttcacgaaca tcgcatctcc aaaactaaag
      181 aaacgatagc gctcttcaat cgcatgctgg taagcagata aagtcaattc tcttcctgca
      241 aaggcactaa ctaacatgac taaagtcgat tttggcaaat ggaaattagt tgagaaagct
      301 tctaccactt tgaattcata gccaggcgta atgaaaatat cagtccatcc gctgtctgct
      361 ttgatttctc cattaaattt cgttccaatc gtttctaatg ttcgaattga agttgttccg
      421 acagccacga ttttcccgcc atttttacgt acttggttca atcgctcagc tgcttcttcc
      481 gtcaaacggt agaattcact atgcatatgg tgttcttcaa ttttatctac gctgacaggt
      541 cggaatgtac ctaaccctac atgtagtgtc agatagacta attcaacacc ctttgcttcg
      601 atctcatcaa gtaattcttt cgtaaaatgc aatccagctg ttggcgctgc agcagaacca
      661 ttttctttcg catagacagt ttgatagcga tctgggtctt ccagacgttc tttgatatat
      721 ggaggcaatg gcatttttcc caatgattca agaatctcta gaaaaattcc ttcatactca
      781 aagtcaatga tccgtccgcc gtgttccaat tcttctttca ctaccgcttg caatctgcca
      841 tcaccaaaag agattcttgt cccaactttg gcacgtttcg caggttttat caatgtctcc
      901 cacgtatctc cttgtgtatt cgttagaaga aggacttcta aatgcgcacc agtatctggt
      961 ttttctccat ataaacgagc aggcagaact cgtgtattat tcataactag cgcatcacca
     1021 ggctgtaact cttcaatgat ttcatggaaa tgtttatctt ccatctctcc aattttccga
     1081 tccaaaacaa gtaaacgaga atgatcgcgg tcttttaatg gtgtttgtgc gatcaactct
     1141 tccggcaagt caaaatcaaa atcttctgtt gttaacatat cgtcactcac tttctatcat
     1201 gtgcttcatc attctatcat gtttttcatt ctgttggcaa aatcagcact ttttcacttc
     1261 tattttttgt tttcctaaat aaaaaaacta ccctatttgt attttttcat tgttttaggc
     1321 atactagtaa tagatagttc ttcattgaaa atagaaaaag aggtggcacc atgattgaat
     1381 ttcaaaatgt gtcaaaaatt tataaaggtg gaaaagtcgc tgtagaggac gtcaatcttt
     1441 catttgaaaa gggtgagttc atttgtttta tcggtacgag cggaagcgga aaaacgacga
     1501 cgatgcggat gatcaaccga atgactgatc caacacaagg gaaaattttg attgatggaa
     1561 aatccatcca agaaattgat ccagtggaat tgcgacgtca gatcggctat gttatccaaa
     1621 gtattggatt gatgcctcac atgacgatcc gtgaaaatat cacccttgtc caaaaattgt
     1681 tgaaagtcag tcaagaagaa cggaataaaa cagctgaaaa aatgattgat cttgtggaat
     1741 tgccccgtga gatgttagat cgctacccac atgaattatc tggtgggcag cagcagcgta
     1801 ttggagtcgt tcgtgccctt gcagcgaatc aagatatcat cttgatggac gagccattcg
     1861 gtgcgcttga tcccatcact cgtgattctt tgcaagacct tgtcaaagat ttacaagagc
     1921 gcttgggaaa aacgatcgta tttgtaaccc atgacatgga tgaagcgatt aagctagcaa
     1981 gccgtatcgc catcatgagt gaaggccgtg tgatccaatt tgatacacca gaaaatatct
     2041 tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg agaagatcgc ctactacaag
     2101 ccaagccaaa tgcgacaaga gtcggagaag ttatgctaaa tacagcaatc acgattacac
     2161 cggaaaaatc attacaagaa gcaatcaaac tgatgaggga aaaacgtgta gatacgttgt
     2221 tagttaccga taattcgaat gtattaaaag gatttatcga tgtagaaacg ttgaataaaa
     2281 agcgaggaaa agtatccagt gtcggcgaca tattaaataa acatgttttt tacgtaaaag
     2341 aatccgccta tcttcgtgat accttgcaac gcattttaaa acgcggactg aaatacgtgc
     2401 cggtcgttga tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca ttagtggata
     2461 tcgtctacga tgtgatttgg ggagaagatc ccgcaactac tgatgttgca gagtcagata
     2521 ctcctgaaga atctaaggag gtctgaccta tgcagcagtt ttttcatcag cacggttcag
     2581 aaattctctc aaaaggtttt gaacatcttt atatttcggg acttgctttg atcattggta
     2641 tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct gccacgattg
     2701 tgattggctt aaccagtgcc ttgcagacaa tcccttctct tgctttattg gcattgatga
     2761 tcccgctatt tggcgtaggt aaattacctg cgattatcgc actctttatt tattcattat
     2821 tgccgatttt acgtaatacg tacattggga tgaaaaatgt cgattggaat tatcgggatg
     2881 tggcaaaagg catggggatg actgagttcc aatccatttt ttccgttgaa ttgcccattg
     2941 ctatgccaac gatcatggct ggtattcgtt tagctgccgt ttacgtgatt gcatgggcta
     3001 cacttgcctc ttatatcggc gctggtggtt taggcgattt gatttttagt ggactgaata
     3061 attaccaacc agatctcatt ttggcaggta cgatccctgt aacgatttta gcactattgg
     3121 ctgattggct tctaggtctc ttggaacacc ggctgacacc gatcgcactt agagaggaga
     3181 atgaagaatg aaacgtatac taaaaatgac catggttctt ttctctcttc ttatacttgc
     3241 gagttgttct ttccctggtc ttgccagcaa tacagatgaa gacaccattt cgatcacagg
     3301 tggaatcact tcagaagcac agatcttagc aagcctagtt gccggaatgg ttgaacatta
     3361 tacagataaa aataccgcaa tcatcaacaa tctggctacc acaactatca accatcaagc
     3421 gatgatgaac ggagatgctt caatttcagc ggctcgctac acaggaacag atttaacgac
     3481 tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg tgaaagatga
     3541 atttgagaaa agatacgatc aaacgtggtt cccgtcctat ggttttgaaa atacgtatgt
     3601 ctttcttgtt cgaaaagaca ccgcacaaaa ataccattta tctaaagtca gcgatctgaa
     3661 aaatgtagcg gatgaattag tagctggtgt agatacttct tggatcaatc gtaaaggaga
     3721 cggttatgac ggcttccaag aaacttatgg cttctccttc aattcgatat tacctatgca
     3781 gattggactt gtttatgacg cagtcgaagc aggaaaaatg gatattgttc ttggctactc
     3841 tacggatgga cggatcgcca gttatgatct agtcatgttg gaagatgatc gtcaattctt
     3901 cccaccatat gatgctgctc cagttgtaaa caacaaaata ttagctgata cgcctcattt
     3961 aaaagaagcc ttaaataagt taggtcatac catcagtacg gaaaaaatgc aggaattgaa
     4021 ctacgaagca gataataatc tagtggaacc atcagtagta gcacaacgtt tcttacaaga
     4081 aaaccactat tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt tcaacaattt
     4141 ttctattatt tccaggaaaa tggctcctat atctttgcac aatttatccg tcacttcctg
     4201 atttctatct atggtgtcct ctttgcagca gttgtcggga ttccggttgg gatcatgatt
     4261 tctaggagac gtaaattagc aaactgggtg attcgtttgg cgaatatcat ccagacgatc
     4321 ccttctctag ccatgatctc cattttaatc attggattag gcctgggagt caatgtcgtg
     4381 attgttactg tatttttgta ttccctctta ccgatcatca agaatacgta taccgggatg
     4441 atccaagtag acaaaaacat ccttgatgtc ggcaaaggca tggggatgac tgctagacaa
     4501 cgactattca tggtagagtt accactctct gtttctgtta tcatggcagg cataagaaac
     4561 gcgttagttg ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc tggtggtctt
     4621 ggagatatca ttatccgagg aaccaatgca acagatggca catcgattat tttagcaggt
     4681 gcattgccaa ctgcattaat ggtgattgtc acggactggc ttttaggtat tttggaaaga
     4741 cggctagatc cagccagccg tacatcacgc tgatgttatt gtaaaaaaca aaagcttctg
     4801 cgaccatttg ttggtcgcaa aggctttttt agttctgtca ttttttaagg caataatcca
     4861 ctctgattct tccctcctta tgggcaaaga gttgcttttc ttagctattt atccattccg
     4921 tatactccaa cttcatactg ccaacgggtt catctctgca tccaccgact aagttttttt
     4981 cttatccgct aaggcatttt gccaatccat tttcccatca ctcgtcatgg aattcatacc
     5041 acatgatctc taaatactcg tctgcttgtt ctttgaattg ggcatcaatt cttacatcct
     5101 ttggcgtggc tttcatttaa gccttttcca tcagtcctcg attgttcgat gccttcagct
     5161 acatcatcaa aactggtgac tgccgtatcg gtaacttcga tcatctttta tacaatcggg
     5221 aacttgccat cctcatcctt ggctaaaaat atatctagcc attccagctt ctcgccatgg
     5281 gaggtttgcg gttcctaaaa ccggtacatg attcttatgg actgcatccg tcgcaactag
     5341 acttggcggt acgatgatcc ttcaccagaa gagcaccccc gtaaaccaat tgatcgatat
     5401 attgccaata agaaaagaca ttggcgtaaa agatatcgat ccctcgtgat gcattgccgt
     5461 tgatactgct attcatggcc gaaagcgcca cgactttgac ttcttccgtt tgagtgacat
     5521 tcatgttcgg caatgtcgct ttggataccc gctctgctaa agaaacgaca ctggtattgt
     5581 atttcgtatc tgtatctttt ccaaaagacc atgccaacga atcctctggg aaccaataag
     5641 acgactccgg ttgattgtcc attgcttttt tcaacatact tttggctgtt tctcgataaa
     5701 cgacatttcc aattgtcttc gcctcacttt ggcgataatc gatggtcgtt gccactgcca
     5761 acactaacaa cgatgctcca attttactga ttttccttgt cacacttctt cctccttgtt
     5821 ttacataaac ccgtcaacat tccattccta aactcaaaca atgacccttc aaaggtggag
     5881 atataggaac aaattaaaga aatcatgcaa taaatccaag aaggttggtt ttagaacaaa
     5941 aaaagagtct cctttttggg gagactcttt gttaactatc taaaaaggtt ggctcttctc
     6001 acttcactca taatgtgtcc aggcagaata aatttccaca actttatttt cctcatcaac
     6061 tttgtaaacc actcgatgct gtctattcag cctgcgtgag tatctgcctt catgtgtagg
     6121 tctcagcttc tcaaaagact gcgtgggcat atatggatct tccttcaatg tctgaatcac
     6181 ttcttcaaat tgtttcttta aattcgattg cttgattttt cttaaatcca ctgtcgcaga
     6241 gtttttgatt gcgactgtat agttactcat caaagagctt cccaatcaat atcatcgata
     6301 ttcgtaaagc cactgtcgtc tttttcgcgg tctcgtactt tatctaaagt acctgtttgc
     6361 tcaagaaaaa gggtttcttg aatcgctctc cagtcttcta gcccaatcaa tacagcattg
     6421 tttccactac gatcactaat aatctcgatt tcagtatgat tttcatttac ctctttcaat
     6481 aactgataaa aatttttacg agctgtgcta ggattcatcg ttgtattagc cattgtctct
     6541 cctccttttt ctgatataat tataacgtac ttgattaagt acgtcaaaaa atacaataaa
     6601 gcacttaaat tcagcaaact tttcctcttt tttacataga gaaaaagacc cctttttagg
     6661 gaggatcttt gtccacgctc acttcttact cctttacgac tagcggtagc caaacgtaaa
     6721 gtaattggta acaacgtatc atctttagca aaggtcgaat ccaaagcctg tgagtctatg
     6781 caacaacacc aatacacgaa ggttcctcgt tgcagtgtga cttgattgat atcttgaata
     6841 acctgaggat acccacgaaa gatttgtaac ggttattcaa aactgatttt ggccgtcaag
     6901 tcttctactg cctcaaaaat gattgcctct ttcgtctcct gccattcatg ttcactatcg
     6961 atttgatact tgcttagacc gtcgggtttg tgaatcacca attaaaaatg gcactgaatt
     7021 atggtaacaa aaaatggtca cttgacgagg ctgagatttc tacttatgaa gacacatggc
     7081 acttaagagt cgaggtaaag gaagaagctg aaaatatagg aggctgtaca ttatttggca
     7141 aaggattcgg aaattatgat caagacatca aaatcaaaat gttttacagc taaataggaa
     7201 gaataataac atttcatacg ctgttcccat ctttaatggc ttatagaggg cacctttagc
     7261 tgggaataag acaaaacaga ccctctagct tcaattaaga gctagagggt ctgtttttga
     7321 ttttaaaact tggtttattt cttcaaatgg tagctatagg tgctgacaac gatattttca
     7381 cgccaagaca atcgcaatct taaccgcaaa cttgtttggt tatggagaat attctgccag
     7441 cgtctgtttg gtacaaattg aggaatcaac acagtagttg tatagttttg tttcgctgca
     7501 tttttactta cgatatctac ataacggata attggatttt caatcgagcg ataagaagag
     7561 tggacgattg aaaaacggac atctgggaaa tgctttttaa attctgcttc gatttctttt
     7621 tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc accgatcgaa
     7681 cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat tcccgactaa tacaagaacg
     7741 gtattcccgt cataatcatg tagttttgca tcattttcta aacgaagctg ttctgctact
     7801 tttttataat gatcatggat cttataaaaa atgaacataa cgattggcat aatgatgaag
     7861 aaaggccaaa tgtcccctaa ccgatagacg aacaaaatcg caatgattgc atatgagatg
     7921 aacgcacctg taatattggc aatcgacttg cttaaccatc tttttccttc ttttttccat
     7981 ttgacgacca tccctgtttg agataaagca aatggaatga acacgccgat agagtataac
     8041 ggaatcaagc gttctgttga gccatggaaa ataaacagca atacgatcga acctagcgct
     8101 agtgtgataa tcccattgga atagcctagt cgatccccac gatcttgata catgtgaggc
     8161 atgaatttat cttttgccag attgtatgca agaactggaa aagcagaaaa accggtgtta
     8221 gcagcaactg ctaaaatcag cgctgtagca aattgaagga cgtaatacaa aataccatgt
     8281 ccaaagacag ctttaccaat ttgagacaaa acagtgactt ctttttcagg aacgatccca
     8341 taccaatagt tgataaatgt gatccccacg aagaagaaac ctaagatcaa tgccatcatc
     8401 gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa agaatggtac agcattacta
     8461 atcgcctcca ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa aatcaaagcg
     8521 atagaaacac ccggcacagc cgctcctgga agtgcagtag cttgcaatgg cacagcacct
     8581 gtcacgatat tatataaacc aacaacgatc agtaacgaga tcacaaggat aaatgtgtac
     8641 accggaaata ataggaacga tgcagattcc ctcagcccac gaagattcag catcatcagt
     8701 agcaaaacga ttgtcacaga aatagcgact tgatgaccgt agagtgctgg caccgctgat
     8761 gtgatcgctt cagcacctgc tgagacagat actgctactg tcaacatgta gtcgatcaat
     8821 aaagagccac cggaaatcaa gccagcattc tttccaagat tctcactgct gacgacatat
     8881 gctcccccgc catgaggata agcatggatg atttgacgat atgatagcgt taatgaaatc
     8941 aataaaataa taacaaaagc ggcaatgggt agcgaatacc agatggctgc agcagacaaa
     9001 gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa tagatgataa cgcatcagac
     9061 gacaataatg ccaaagcagc aaatcgggtt aatttgtgct catcattttc agcagatttt
     9121 aaaggcttac ctactaacaa cctttttaag taatccaatt tctaaactcc tttacacaca
     9181 attttacttc aagttttata gtcgaaaatt tatagtaaac aagatgttat agtacgctca
     9241 ctgaccttaa aagtcaataa actttaagaa aataattgga aaaaaacaga aaaatatcaa
     9301 gcgctttttc aaacgcttta atacagcaaa aatctaacat ttttttatgg gctttttctt
     9361 tgaattctat aataaaagga tagaacttga cttagaaaat caagttctat cctttttgat
     9421 atcttaactt ttgaaagaga gatgtaaggt tgctttctac ccatcattct tactactcga
     9481 agcatagcgc agttgtcaca acctcttgat aaacggtgtt taaaagcttg catctgcggt
     9541 aataagccca accaaattaa aaacataaga agctgttttt atttggtcgt tcttattact
     9601 tgatgcagaa cagccttttc acaacctctt ggtacacggt gttcaatcag ctgaccctcg
     9661 gcattagctc aaaaaacggc aaaatatgag gaactatttt tccatttttt tctctaatac
     9721 tcaggtcaaa acgctgattt cacaacctct ttttttaatg aaaaatatgg ctctcatgcg
     9781 ccgcttcttt gaatttcgtc attttttctt cgatgaacgg atagattgcc gttcctacga
     9841 ttccaaagac aacaaacaga ccgatcatga tccagattgc tttcgtagca tttggccagt
     9901 aaatgcctcc tgcagattcc cgtaacaagt ttaccgcatg agtaaatggc aagaacggat
     9961 tgatcatttg gaagaatttc cctgagactt ggatcggata gttcccgcct ccgccagaga
    10021 tcgacagtac taagatgata atagcgatcc ctttaccgac attatcgaat aatgcagcca
    10081 agacatagac gatcatcatg aacgctaagg cgatcaagac agcaaatgac acgctgtaga
    10141 ccggattttg gacatctact cctagtaaga aaatattccc taatgtaacg atcaagcttt
    10201 gcgcaatcgc cattgtcaaa aatgtcagca tacgagcaac gaattgttct cgtttcgtaa
    10261 aattgtttcg gtctttcttt cctaaatagt attctgtcgt agcaacactt gaaagaagaa
    10321 cagcgccgac ccacagacat agtgcagtat aaaacggggt gctagctgaa ccgttatttg
    10381 cgattggata aagcgcattg gtttgtagct caacaggttg tgtaaagaag tcactttctt
    10441 ttgttgcatc tagtttcaat aatttgatga cttcgcctag atcgacttct tgttcgcctt
    10501 ttcggatcgc gtctgctgct ttttggatac cagctttgat atttggccag tcattgttga
    10561 tcaagtccgc agcagcgttc acagctgttt cgacttctgg cattttttca tttactgttg
    10621 tcaatgtacc ggtgatttca ttttttagtg ctggccaatc attttggata aaatcagcgg
    10681 ccaatcctaa tttttgctga aggatcggta attcattgtt gtataggtct gccccttcat
    10741 tgatgccatt gacgatggta tccatatttc cattcagcat tgtattggca tcatggattt
    10801 cttgtccaat tgcaggcatt tctttttgat atttttctaa aattgccact gcattcgtta
    10861 cagttgcttt cgttgaactg agaagcgaat caaagtcgat ctggtccgct ttgcttagcg
    10921 ccccttgcgc agtcccaatc gtatcgatga cttggtctaa tagagaagtg acagtggaac
    10981 tgatttgacc tgtattgata ccagcaatga tgccattgat attattaaca acggcatcaa
    11041 tttgatctag caattcatca atagccttcg tgtcttcatt ttgaacagct gtgttcaaag
    11101 cagataggcg gttttgcaga ttgcttagca agggttttaa ttgatttagc tgatcaataa
    11161 ttggctgcaa tttcgtattt ccagcacttg tttgcaattt agtcaggaac tcgaccagtt
    11221 tatcgattgc attttgttgt tgagatattc cgtcaataaa gccgttcaat acattgctga
    11281 tgtttttctt ttcatcatca gtcagcttac catcttcaat agctgttctt acagtatcta
    11341 ttaacgaacc tgtcgttgtt gccacttgtc caattgattt caatgtaaca tctacactgt
    11401 ttgtgatgct tggtatcgca tctttcagct gagaggcacc tgattttgtt tggtttgcta
    11461 gatcacttgc ctgattttct aaatttttca catctggcaa gatatcctgt acttgttgga
    11521 tgatcgtcaa gccttgtttt gcttcatcga ttccttggtt cattgtctct tggatcgaag
    11581 caaaatcgcc gtcgatctct gctagttgtt taccggcatt ttggatctct gggatttttt
    11641 cttgcaaatc aaggatgatt tttgcttgat ctttcaactc aggcattttg tcattcaatg
    11701 caacgacttt ttcacccatc tcatctactt tagggatata atccacgaat tcatttgctt
    11761 tattcagttt ttccttgatt tcaggtagtt gactttgtaa ttcgaggact tgttttgtgt
    11821 agccgtcgat cgtgtccaaa ttttcatctg ttgataaaat catatctttg actttattga
    11881 tacttactag gttcgtatcg atgtcgtaac ctatctcatt gaatactttc aataaagtcg
    11941 aactggctgt tttgataaat tcatttgtga tttgcgattg gatcgaactt gctcctttat
    12001 cggtgatttt cggtgcaatc gcattgattt tttcatttac cgtatattcg atttttggtt
    12061 tcttgatatc accactagta aaactcagca gatcttctga gaaatccttt ggcaagtaaa
    12121 tacctgcgta atactttccg gaacggactc cgtcttctaa ttgttctttt gaatcaacga
    12181 attgccatcc taattgttta tttttatgaa gcgattcgat gacttgctcc ccaatggcca
    12241 cttctttacc ttgaaactct gctggcttgt ctgcactgta aacagcaatt ggcagctctc
    12301 cagtatttcc atatggatcc cataatgctt tgatgttaaa ccacgcatat agcgaaggga
    12361 tgatcatgat cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc cgtttccagt
    12421 caagcaaata taatcgaagc gtatttttga ttgactgcat agttctcccc cttcttcttt
    12481 tttcagctga attactttac catgtatttc atactttttt caatcggctt ttttcaaaaa
    12541 agtcgaattt ttggacatat cacggcaatt gtctcattgt aatctcttac atattgagaa
    12601 ataattaagg attacagact ttactttatc atgaaataaa agggaaagat aattttatgc
    12661 caaagttttt atct
//
LOCUS       DACEUF010000074        12672 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.077, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000074 DACEUF010000000
VERSION     DACEUF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.077"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..157
                     /locus_tag="IXG20_002076"
     CDS             <1..157
                     /locus_tag="IXG20_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1610261.1"
                     /translation="RKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQM
                     LSQPMEN"
     gene            692..1264
                     /locus_tag="IXG20_002077"
     CDS             692..1264
                     /locus_tag="IXG20_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1610262.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDAFDEDED"
     gene            1539..2156
                     /locus_tag="IXG20_002078"
     CDS             1539..2156
                     /locus_tag="IXG20_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006845390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1610263.1"
                     /translation="MDRIAGKAHTKHYQKGEFLFRSQEKDDALYIVNRGKVRIYRLSN
                     SGKEQLVRILNPGDFTGEWTLFNPDAVHEEYAEATRDTSVCMIQQHDVQEFLKEYHAI
                     SLKLLGEMSQRLESSGRQTAQVAVESVITRLVLFLAENVGPEMGNSPTITLPMAKKDI
                     ASYLGTTPETISRKFGELEDEGLIQQLSGKRIKIQDLDDLLLYSE"
     gene            2308..3162
                     /locus_tag="IXG20_002079"
     CDS             2308..3162
                     /locus_tag="IXG20_002079"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1610264.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAAPQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILTIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWVNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIIVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3309..3542
                     /locus_tag="IXG20_002080"
     CDS             3309..3542
                     /locus_tag="IXG20_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAQ1610265.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGVDKDSIKVLFNSSKV
                     KANFDSAVTSIEEIQKAIENVGYPVLKAKVKAA"
     gene            3740..5644
                     /locus_tag="IXG20_002081"
     CDS             3740..5644
                     /locus_tag="IXG20_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1610266.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAVPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDIKRFEQHGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGHMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5954..6316
                     /locus_tag="IXG20_002082"
     CDS             5954..6316
                     /locus_tag="IXG20_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1610267.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6333..6944
                     /locus_tag="IXG20_002083"
     CDS             6333..6944
                     /locus_tag="IXG20_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HAQ1610268.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(7046..7954)
                     /locus_tag="IXG20_002084"
     CDS             complement(7046..7954)
                     /locus_tag="IXG20_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1610269.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(7996..8310)
                     /locus_tag="IXG20_002085"
     CDS             complement(7996..8310)
                     /locus_tag="IXG20_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1610270.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8609..10540
                     /locus_tag="IXG20_002086"
     CDS             8609..10540
                     /locus_tag="IXG20_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1610271.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10833..11384
                     /locus_tag="IXG20_002087"
     CDS             10833..11384
                     /locus_tag="IXG20_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1610272.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            11697..12239
                     /locus_tag="IXG20_002088"
     CDS             11697..12239
                     /locus_tag="IXG20_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610273.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT         4112 a         2131 c         2634 g         3795 t
ORIGIN      
        1 ccgcaaggaa cagttccaaa atgaagagtc gatggaacgt ttcttagtct catcttttga
       61 tacttacaac caaaaattcc taggtcgcag tcataaaggc ttccaacagg ccgaaggcga
      121 gctcgaacaa atgctgagtc aaccgatgga gaattagaaa caatctacag tagggaagaa
      181 ccatttacac aaaattattg acgctcccaa gctaaaactc ctattaatcg atctactaaa
      241 aataaataaa aaaattcttt tcaattttta tttatggtgt gacataaata aagtaaagtt
      301 attcttctag gttaatctta ttaatgctct ttctcactag tgttgcatgc aacactagtg
      361 agaaagagta ttaaatatac tgcaaggtaa gacatttggt aattgaagaa taacaagcaa
      421 aaaaatagca gtcaggtctc ttgacaaagt atagttgcta ttctttttta ggccctactt
      481 taatgaaatt tttgtttgga gggaacgact tcattccttt gattatttca gttgaattta
      541 atagagaagc ctgtcgtcta ttaagaaata cttttcttga tgagcgtcaa tttttttaac
      601 ggaaatctat cctatactaa attcagtaaa gaaaaaggag gaatcaaaga aagctatttt
      661 aaacaaacca aaattatagg aggaataaat catgactgaa aataaagcta ctcaagaaag
      721 aacaccccaa gaaagattgc aagaagaaca agaacacaag gaacacgttc atcatacgaa
      781 aattaatgca gcggccattg cggatcatct tttaggcaac atgcatacat tacatgtgaa
      841 actgcaccaa tatcactggt atgtaaaagg agccaatttc tttacgttgc atgaaaaatt
      901 tgaagagttg tataacgaaa atgaaaaatg gttcgacaaa cttgcggagc gtctaattac
      961 ttctgggcat aaacctgctt cgacaacggt tgaatttgaa aaatattcga tgctttcgga
     1021 agatgcagtt aataaatacg ctaaagcaga agacatggtt gaaaatatta tcgaggactt
     1081 cagaagcact cgtgacctaa ctattcgcgc gattcgttta gctcaagacg agggtgacga
     1141 tgctttagaa gatacattga ttgcttttaa aaatgactta gacaagaaca tttggatgct
     1201 gcaagcgttc attggcaaag aggcattgga agacgacgat gcgtttgatg aggatgaaga
     1261 ttaggttttt cgtaaacata catttaaaaa ttaaagattt tggggttggg actctttcca
     1321 gctcccattt ttttgccgtt aaaaagactt ttcttgatga ttgtcaattt ttttgaggta
     1381 gggggccttt atactaaata taggaaagtt aaaagaagga caataaactg ataaagggga
     1441 agaacgaatt gactcaatac acccaaccac atgcagcagg agaccactca gcttgtatcc
     1501 gcttagtccc gatattcaat catttggagg aaagctcgat ggatcgcatc gctggaaaag
     1561 ctcacacgaa gcactatcaa aaaggggagt ttctgtttcg ctcgcaagaa aaagatgatg
     1621 ccttgtatat tgtgaatcgc ggaaaagtcc gcatctatcg cttgtctaat tctggcaaag
     1681 agcaactagt tcgtattttg aatccgggtg actttacggg agaatggacg ttgttcaatc
     1741 ctgatgctgt gcacgaggaa tacgcagaag ctactcgaga tacgtctgtc tgcatgattc
     1801 aacaacatga tgtacaagag tttctaaaag agtatcatgc aatttcgtta aagctgctag
     1861 gagagatgtc acagcggtta gaaagctccg gacgtcaaac tgcacaagta gccgtggaga
     1921 gtgtcattac ccgtttagtt ttgtttttgg cagaaaatgt gggacctgaa atgggtaact
     1981 ctcccaccat caccctgccg atggcaaaaa aggacattgc ttcctattta ggaacgacac
     2041 ctgagaccat cagtcgcaaa tttggagaat tggaggacga gggattgatt caacagctgt
     2101 ctgggaaaag gatcaagatt caggatttag atgatttatt gctttacagc gaataatcgg
     2161 acacggaaaa gtggcgtatt acgaagcgag tgaccaggga gctaaaagtg aattcactgt
     2221 ttggtttccc tggaggttag ccggaatctt gttcagcatc accatgacta gaatgacgcg
     2281 aaaaattgaa gaaatggagc aaatagaatg ggattcgtat taggcgtttt gaatccccat
     2341 gtggtggaga ttggttctgt tgtgattcat tggtatggag tcattattgc agcgggcata
     2401 ttagcggctc cccagttgag caccaaggaa gcaaagaaaa aaggactgga agaggatacc
     2461 attatcgata tggccttatg ggcaattccg attggactcc tcggcgctcg gctttattat
     2521 gtgttatttg aacttggtta ttatctgcaa aatccaggac aaatccttac gatttggaat
     2581 ggcggtattg cgatttatgg gggattgatt gcaggagggg gtacgctata ttggtatgca
     2641 aagaaaaaag gtacttcttt ggcactcgtt cttgatattt tagcgcccaa tgtattgtta
     2701 gcccagtcca ttggtcgttg ggtcaatttt atgaatcagg aagctcatgg tgggccggtt
     2761 actcgtcaat tcttggaaaa tctttattta cctgaattta tcattgaaca aatgaatatg
     2821 aatggtacct actaccatcc gactttttta tacgagtcat tgtggagttt gttgggcttt
     2881 gtcctcatcg tcactatacg gaaccgaaaa cagcttttgc gtcaaggaga agtcgctttg
     2941 agttacgttt tgtggtactc agtgggtcgg ttcttcattg aaggtatgcg aacggacagt
     3001 ttatggattg gcgagtggat ccgcgtttcg caagccttgt ctctggccct gtttattggc
     3061 gcgattatag tatggtttat acgcagacaa gattatccac caatttctta ttattctgat
     3121 ggcaataagg ggcagaaaaa gactattaag atggtgaatt gagcaaaaag ataaaaagaa
     3181 gtgcaaaact tgatatgcgt caattttcgt ttgctaaaag ggagctatac tacagttgta
     3241 aagaggaaaa ggcagtgttc attaatctgc tgccgataaa ataaaaacac ataaaggaga
     3301 taggaattat ggaaaaagct atattgcaat tagaaacgtt gtcttgtcca agttgtatgc
     3361 aaaaaattga aggtgccgta aaatcagtta acggtgttga taaagacagc attaaagtat
     3421 tattcaactc cagcaaagtc aaagccaatt ttgattcagc tgtcacttcg attgaagaga
     3481 ttcaaaaagc tatcgaaaac gtaggctatc cggtccttaa agcaaaagtc aaggccgctt
     3541 aacagattaa atgaactagg caattctgga agaaaagcta tttttccagg attgcttttt
     3601 ttgttgcaat ccgattcgag gtgcttagag acgataaaag agaactttct aaacttgatg
     3661 tcagtcaatt aattacttag gcaattatct tatactgtgt ttatcaataa aagagaaatt
     3721 aaatttggag ggaaaaagca tgcaacaata tatattaagc aagaaaaatg ttatcaccgt
     3781 catcagtgga ttgttaatcg tactcggttt cttcagtcac tttgttttag aaaacgtagg
     3841 actttcagag tggtctttga tcatcgcctc tgtctttggg attacgccaa ttgccattca
     3901 agcgtttcaa gcaatgaaag tcaaagtcat cagtattgat gtcttagtca gtattgcagc
     3961 gattggtgcg ctttttattc aaaattatga agaatcggct attgtcacat tcttattctt
     4021 attcggacac tacttggaac aacgaacatt gaaccaaacg cgatctgcta tcaaagaatt
     4081 gactgaaatg gcacccgaaa gcgccttgaa acaaatggat aatggcgaat ttgaagaagt
     4141 agaagtggat gatatagatg aaggggatat cttgctcgtt aaaacgggtg cgaaagttcc
     4201 agtagatggc atagtcctta cgggtgaagg gcatatcaat gaagctagca ttacaggtga
     4261 agctgttcca gtcaacaagc tagctgatgc agaagttttt gcaggaacca ttttagaaaa
     4321 cggaacgatt caaatcagag ctgaccgagt tggtgaggac accacatttg gaaaaattat
     4381 tgaactcgtg gaagaagcac aagattctaa atccgaagcg gaacggttca ttgatcgctt
     4441 ttctaaatac tacacaccag ctgtcttggt tctggcaatt gttgtatggg cgttcagtca
     4501 aaatattgag ttagcaatca ccatcttggt tctaggttgc ccaggcgcat tagttatcgg
     4561 agtgcctgtc tcaaacgttg ccggtattgg gaatggtgct cgtaacggtg ttcttttaaa
     4621 agggagtgaa gtcattcaag acttcagcaa tgtggataca attgtatttg ataagacagg
     4681 tactttaact gtcggaaacc caaccgttgc agcgactgaa ctgtatgaaa aagatcctgc
     4741 tgaaacgctt ggctatctgg ccagtgtgga acgggaatca gatcatccat tagcaaaagc
     4801 ggtcttaaat caaattggag aaacaaactt ttatcctgtt gaaggaactg aagtcgtgaa
     4861 aggcggcgga atcgtttcaa gtgtagctgg acatagagtg gctgttggga atgtggcctt
     4921 gatggaaaaa gaaaatgtca ctctcagcaa aaaggtgcaa aaagatatca aacggtttga
     4981 acaacatggg aactctctcg ttttgacagc ggtcgacggt gaattaaaag tactgatggg
     5041 cattcgcgat caagtccgtc cgggtgtgaa agctaatttg caggaattaa aagacttggg
     5101 cgtgaaaaat ctagtcgttc tttcaggaga taaccaagga accgttgatg tggtcgccgg
     5161 cgaacttggt ttgaccgaag ctcacggcca catgttgccg gaagacaaat cggcttatat
     5221 cggaaaactt caacaacgtg gtcaaattgt agcctttgtt ggagatggcg ttaacgatag
     5281 tccgtcctta gctttagcag acattggaat cgcaatggga agcggaacgg acgtagcgat
     5341 tgaaacatcc gatgtcgttt taatgaactc gaacttcagc aacttgcctc acgcattagg
     5401 attagtaaaa gccaccgcaa ataatatgaa acaaaatatc gtgatttcaa ttggagtagt
     5461 gttggtcttg ttgaccagcg tcttcttcag cgaatggatg aatatgtcta tcggaatgtt
     5521 ggttcatgaa ggtagtatct tggtggtaat tttcaatggc atgagattaa tgaagtataa
     5581 gttgaaaggt cgaaaagaac aaaaagaagt atcagcacct gcagagaaag taaaaatatc
     5641 ttaagaataa attagaagag gctgagacaa tagtccatcc ttgaaatgac aaaagcggaa
     5701 actcagattt ttaaatctgg gattttccgc ttttttgagt tcttgaaaaa aaataagagt
     5761 atagagtatg ttctttataa aattaatttt cgtgtaaatg attaaaaaat ttaataataa
     5821 aatttaaatt cctattaagt tgtaacacat gaaattgctt ataatagtgt attgaaatta
     5881 aggtagattg acaaacgagt gatcaaatag atatactacc ttcaaacgac cgtttgaatg
     5941 tgaggtgaga tgaatggaaa aagtttgcca agtgactatc gtacataaag aaaaagtaaa
     6001 tgtaataaaa aaaaaattgg atcaaaaaga tttttcaaac ttacttgtgt tagggaaatg
     6061 tttcagcgat tcttctagga ttaaaatttt ctatgcttta gaaacctata aggaaatgtg
     6121 tgtctgtgat ttagcagaaa tacttactgc tagtgtcgct acaacgtcac accatttacg
     6181 ttttttaaaa aaacatggaa tggcaaaatc acgtcaagat ggaaaagtag tttattattc
     6241 tttagcgaat gaagacatcc tttccgctat tcgtgttttt ttaaagttat cagaaaatct
     6301 atctatgaaa tcttagtttg gaggaagtta ttttgttaca aagtattttt tcagctatcg
     6361 ctgtttatat ttctaccagt attgattatt tgtttatctt gctgattatc ttttctcaaa
     6421 gccacacaaa aaaaggtatt cgtcacattt tttggggtca atatcttgga acaggtattt
     6481 tggtagccgt aagtttgttt gctgcttatg tgctaaattt tattccgcaa gattggatca
     6541 ttggtcttct cggtctaatt ccgattttct taggaatcag agtcgctttt gttggcgaag
     6601 aggaggaaga agaagaggaa gtcgttgaaa aactcgaatc tagaggaact aatcgcttat
     6661 tctggacagt tgccttaata acgattgctt ctggaggcga taatttaggt atttatattc
     6721 catactttac ctcgttagct ttttcagaaa tcattattgc tttaatcgta ttcgctatct
     6781 ctgttgcggt tctctgctat atcagttaca aattagccaa gatttctttt gtttccgaaa
     6841 cgttagaaaa atacgaacga atcattgtcc ctataatgtt tattgcgtta ggaattttta
     6901 ttatgataga gaacgggacg atacaaacgc tactaggttt ttaaaaatca taatcacgaa
     6961 agataaaaag agaggcagcg actaatcaaa atcgctgcct tttttgcttt tcaaataatg
     7021 aagtattttt tagcgtagaa ctatcttacg tgttgtggga gtatcccttt tcaagctctt
     7081 ctttgcttgt agttatttct attgtgatat gattctctac gcttgatttg tgtgcgactt
     7141 ctgcaatttc ttcttttatt tccctagcct tgtctatgtc tgaagggtct ataagaaccg
     7201 tgatgataag ggcgttttct tccccgtcca gtgaccaaat gtgaaaatgt gagaggtctt
     7261 ttacagcagg aacattttta atggagtccg ccaagctctt tacgtttaca ttttcaggcg
     7321 aaccattcaa taaaattttc attgtggtga aaaattcagg aacggttttg tagaggatat
     7381 aaagtgcaat caaaatcgat aagataggat ccagacgata tgcctcggta aagttcatta
     7441 gaatactcac aatcaagact ccaatccacc ctaatacgtc ttccagcata tggaggttca
     7501 acatgctctc gtttttggat gtgcctttgc ttaaaatcca cgcagcatat ccattcaatg
     7561 caatagctac gagagaaagc cagaacatgc cactataatt tacgggttgt gggtcaatca
     7621 aacgtggcac actacgatag atcataaaaa acgagccact aattagcaca acgccagtta
     7681 tcagcgcgcc cagtaaagaa aagcggttat agccaaaagt gaaccgttca tcttgttttt
     7741 tctctgaata accttgaaaa aaccaagcga atccaattga aatagagtcc cctaaatcat
     7801 gaactgcgtc ggacataatg gacacactat tgaaaagaaa gccaaagaaa aattcgctta
     7861 tagagaaaat aagatttatg aagaaagcaa ttttaatatt ctttttcggt tgattctgat
     7921 tcgagtgatt atggtcttta tctgattttg acatttaaaa acctcctaag tttgtatgct
     7981 acatactaat tggttttatt tttctaattc attaacatga gttaagactt gttcgagaat
     8041 actaaaaaca tgaaggtcat ctaaactgta aagcatactt tttccttctc ttcttgattt
     8101 cactaagcgt gcgtctttca acgtttttaa ttgatgagaa atagcggact gttccatgtc
     8161 cagagaaata gcgatatttc caacactaca ctcttttttc tgtaaaagaa agagaaccga
     8221 caatcgtgta ggatcactaa tcaccttaaa tagtttgctt attgacagaa tagatccttt
     8281 atcgattgga gaagttgttt ttatatccat agtgttttta ttcctttcat atgaccttcc
     8341 gttcatatct atattaccat atgagcgaaa caatgctaat cttttgttta taaaagagaa
     8401 gtggcgttct ctttatcttg acaaaatgta tatagcgata taaaatgagt atacaaacat
     8461 atgattaatt agtcatatgt aaggtggatt cattacgatt tacttttgga ataaacgaaa
     8521 ggagtcagaa atagcagaaa tttatgtgat tcaagaagta aatgatggaa aacaaaggaa
     8581 ttataaatta tatagggagg caatagaagt ggaaactatc caagaacaac agtctcaaga
     8641 aaagcataat cataatcaca accacgatca cgaccatgat catgggaaaa tgccagtcgt
     8701 tttgtacttt gttggtttag cattagcagt gattgctcta tttttgaatg aagattatca
     8761 gttggtacaa aatgttttgt tctcaatcgc tacaatcagt gccgggtatc acgttattct
     8821 tcttgaaggc cttggagaaa caattgagaa ttcaaaagca caaaaaaagt tcatacctaa
     8881 ttctcatatt ttaatgggat tagcggcaat tggagcttct ttgatgggga atttttggga
     8941 aggaacatta ttgattctta ttttttctgg cgcacatttt ttggaagatt atgcagaagg
     9001 aagaagcaaa cgagaaataa cgaaattact cgaaatgaat ccaacgactg cccggttaat
     9061 cctgcctgat ggtagtacaa aaaatgtgga tgtcagtgaa ttaaaagtgg gagatcaact
     9121 tcaagtgttg aatggcgatc aagtacctat tgatggcgtc attttgtctg gatctacatc
     9181 cattgatgag tcgtctatta ctggagaaag tatcccgaaa gagaaatcca aaggagatgg
     9241 cgtcttcggg agtacaatca atggaacagg taccttcacg atgaaagtca ccaaagaaaa
     9301 caaagataca gtattttcaa aaattttaca attagtgaat cagaaccaag ataaccaaac
     9361 aaaagctgcg agtattatcc aaaaatttga accgaaatat gtcacagttg ttttaattgc
     9421 cattcctcta ttcatgttat tggctccttt tctacttgat tggacatggt cacaaagtgt
     9481 ctatagagga ttggtccttt tagttgcagc ttcaccgtgt gctttagcag cagctactgt
     9541 ctcggcaacg ctatcgacga cttctaattt agccaaaaaa ggagtccttt caaaaggtag
     9601 ttcttacctg tctcaattag ccgatattaa agcaattgca tttgataaga cgggcacctt
     9661 gaccaaagga aaacctgaag taaccaatta ttattttgct gattccgtga acgaagaaaa
     9721 catgattgat atcgtagtag ctcttgaaaa agaatccaat caccccttag cagatgctat
     9781 tctaagaaag tttgagcaaa aaaacaaact aactattgaa gtagaaaacc aaattggtaa
     9841 aggattgacg ggagattaca aagggagaaa ttatcgtata ggaaaaccga cttcgtttga
     9901 tgatgtttca gatgaatata gtcgtttaaa taatgaatgg gcgtcagaag gtaagacagt
     9961 cgtatacgta gcagtagatg aagaggttat aggccttatt gcccttatgg acgttccgag
    10021 tgaatatgct aaagaaacca ttgaatattt taggaaacaa ggtatacaca ccacattaat
    10081 tactggtgac tcagaaatga cgggaaaagc ggttgctaaa caattaggaa tagatgaagt
    10141 gattgcgaat gtcatgccgg aagacaaatc cagaattata gacgaacaaa aagaaaaata
    10201 cggcgtgacc gccatggttg gagacggtgt aaatgatgct cctgctcttg ttaatgcaga
    10261 tgtgggaatc gctatgggag atgggactga tgtggcagta gaggtatctg atttagtttt
    10321 aatgcaaaac aatttatcta aattggtaca atctcataat atttcttcga aaatgaaccg
    10381 tgttatttgg cagaatatta ttttttcaat ggccgttgtt gcttttttaa ttgtcgttag
    10441 tttcttaggc ttaacggata ttgcaatcag tgtcattatt catgaaggaa gtactttggt
    10501 tgttattttg aatggacttc gattgctaaa ggcagtctaa agaatgattg cacaacttca
    10561 aattaatcga gtaatagaga taattaagga ttacactaca ttttttatct ctattacttg
    10621 tttatattta tctagatgtt caaagtcaaa atgatatatc aaccatactc ttgtggcgcg
    10681 ggttgaatat aagatacatg aaattggata aaatcatgta tcaagaatcc tatccttata
    10741 gggttctttt tttttactcc ccctaaacac acgatttcgt gttgtaataa ctataatcat
    10801 gtattcgaac agactaaaaa ggagggattg tcatgggcta taatgttcag ccactacggt
    10861 cgcaacagga gattaatgat tttttatttt gcctgcgacg gaataaaaat gcggaacgtg
    10921 acgttttctt gtttctaatt ggaattaaca gcggtctgcg catgtccgac atcgttaaac
    10981 tcaagaaaaa agacgtgatt acctcaaaaa acccgcgcat cgtggagcaa aagacaggga
    11041 aaacacgcat tttatactta agcagcttac aggacttgat tcacggctat accgcaccct
    11101 tagatccgga ggcttacttg tttcccagta ctaagggcgg ccatttagaa gtcaatacgg
    11161 tctaccagat gtttcaaaag gtcgcgaagc tgttaggaag ggacgatatc ggcacccaca
    11221 ccctacgaaa aacctttggt taccactatt acaagaaaac caaagacgtg gccaccttaa
    11281 tggaaatctt tggtcacagc agcgagaaga ttaccaaacg ctacatcgga attaatgaag
    11341 atgaaatcag tgaaacgtta ttaaattttc ggttaggttt ttgattgtat tatataatag
    11401 ataagcacag taagacggtt ataatcaccc acagtattgg acaaatcaac ctttttttgg
    11461 tatattatat atacaaatga gagcaggctc atagtcaact tgatataaat ccggaacata
    11521 agggtccggc gggtggcgag caccaaaact aaactcgata caaatccgga acgtaagggt
    11581 ccggcgggtg gcaagcacca aaactaaacc tgtacaaaag gctgctcttt tgagcagctt
    11641 tttttattgg gaaaaaggag cattcttcgc acgcggcaaa aaaagaatac aaacgcatgt
    11701 ctataaaaga gacaacccaa attaccagac agttaaacgc gatttataag gctgctcatc
    11761 tcttacaaga acattttgta gataaaaaag tatcctttgt cggagaagtc tcaacggtag
    11821 cgattatttt ttcgacaact aattttatgc acctctgtgg cattgattat cgcagaggca
    11881 cacacttgtt ttttcaagat gccttagaca ggaaaataaa tttacaggat attcaaatca
    11941 aaacggatgg gacgaccttt cagaaattac aagtaatagg tagccttgat ttattattgg
    12001 ggaagcatat ttctattgtt ggcagaggtg tttactcttc tcttcgctat gatgcagcta
    12061 ttcgaacaag gaaaaaaata ttggctctgt ctttgaaaca aaacggatta atctatatcc
    12121 ctatctcttt gttaaaccta tcatcaaagg aaattggacc tggacaaaaa gttactggca
    12181 tttttagcga ggatttaaca tccggggaac ttaaaatgat tatggaagtc atcgattaat
    12241 cattaatagt gatatgcgac gtcattttga agtaattatt ctattttggg ttataaaata
    12301 aagcatttta agacgatttc aaatcattca tgtgggttat ttatcacgga tgagcttgaa
    12361 atcgtctttt atacgtatca aattgaaatg ttcggcatac aacctatcgt tgttactaca
    12421 ttactaattg tcacttgtta aggaacttgg atcctttcta aacattccct agagataaat
    12481 tttactttat ccctccgtta atatccaatt taagcacctt tccgattcag ggttaatttg
    12541 catatcaagt gcagcttgtg ttctagtaag attagccctt ttagtccggc tgagttgact
    12601 tatggatata agatatggtc atagcaaata gatactacgt atagtctgtt aatttagtgt
    12661 cattaatctt aa
//
LOCUS       DACEUF010000075        12670 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.078, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000075 DACEUF010000000
VERSION     DACEUF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.078"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            6..305
                     /locus_tag="IXG20_002089"
     CDS             6..305
                     /locus_tag="IXG20_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1610274.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IXG20_002090"
     CDS             378..1739
                     /locus_tag="IXG20_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1610275.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IXG20_002091"
     CDS             1761..2021
                     /locus_tag="IXG20_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610276.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IXG20_002092"
     CDS             2035..2787
                     /locus_tag="IXG20_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ1610277.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IXG20_002093"
     CDS             complement(2881..3486)
                     /locus_tag="IXG20_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1610278.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IXG20_002094"
     CDS             complement(3539..4513)
                     /locus_tag="IXG20_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ1610279.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IXG20_002095"
     CDS             complement(4891..5670)
                     /locus_tag="IXG20_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1610280.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IXG20_002096"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IXG20_002096"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1610281.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IXG20_002097"
     CDS             7161..8558
                     /locus_tag="IXG20_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1610282.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IXG20_002098"
     CDS             8639..9079
                     /locus_tag="IXG20_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1610283.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IXG20_002099"
     CDS             9227..9553
                     /locus_tag="IXG20_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1610284.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IXG20_002100"
     CDS             9554..10303
                     /locus_tag="IXG20_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610285.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IXG20_002101"
     CDS             complement(10383..10838)
                     /locus_tag="IXG20_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1610286.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IXG20_002102"
     CDS             complement(11265..11540)
                     /locus_tag="IXG20_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610287.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IXG20_002103"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IXG20_002103"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ1610288.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4056 a         2175 c         2587 g         3852 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACEUF010000076        12297 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.079, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000076 DACEUF010000000
VERSION     DACEUF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.079"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="IXG20_002104"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="IXG20_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ1610289.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /locus_tag="IXG20_002105"
     CDS             complement(740..1462)
                     /locus_tag="IXG20_002105"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ1610290.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /locus_tag="IXG20_002106"
     CDS             complement(1598..2479)
                     /locus_tag="IXG20_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ1610291.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IXG20_002107"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IXG20_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ1610292.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="IXG20_002108"
     CDS             3719..4198
                     /locus_tag="IXG20_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ1610293.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="IXG20_002109"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="IXG20_002109"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ1610294.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..5627
                     /locus_tag="IXG20_002110"
     CDS             5499..5627
                     /locus_tag="IXG20_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1610295.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5631..6176
                     /locus_tag="IXG20_002111"
     CDS             5631..6176
                     /locus_tag="IXG20_002111"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1610296.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6629..8320)
                     /locus_tag="IXG20_002112"
     CDS             complement(6629..8320)
                     /locus_tag="IXG20_002112"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ1610297.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="IXG20_002113"
     CDS             complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="IXG20_002113"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ1610298.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9802..10821)
                     /gene="argF"
                     /locus_tag="IXG20_002114"
     CDS             complement(9802..10821)
                     /gene="argF"
                     /locus_tag="IXG20_002114"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ1610299.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="IXG20_002115"
     CDS             complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="IXG20_002115"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ1610300.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3374 a         2543 c         2210 g         4170 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttgatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgcgcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccgcccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgcg tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattga
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaaatttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa cttctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcagc tccttcgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatag
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccgtctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaagagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgatttgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactgga
     5041 aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtgctac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa tggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaacc caactgactg acaaagaaat ccgctaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gaatggattt aatgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa
     5941 cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaagatc gtttaaaaca
     6181 gtctgttgct cttagattcc aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga
     6241 gttaacagaa tacattttgt acgctgacaa gaatccagaa tgacttttat acaaaataag
     6301 aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa aatagctcct
     6361 catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
     6421 cgaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
     6481 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
     6541 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
     6601 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
     6661 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
     6721 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
     6781 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
     6841 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
     6901 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
     6961 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
     7021 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
     7081 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
     7141 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
     7201 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
     7261 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
     7321 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
     7381 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
     7441 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
     7501 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
     7561 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
     7621 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
     7681 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
     7741 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
     7801 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
     7861 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
     7921 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
     7981 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca
     8041 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
     8101 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
     8161 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
     8221 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
     8281 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
     8341 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
     8401 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
     8461 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
     8521 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
     8581 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
     8641 ctagtcgttt cataaaaaaa aaatccagat ggcctaaagg agaagaaata ccacctggat
     8701 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
     8761 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
     8821 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
     8881 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
     8941 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
     9001 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
     9061 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
     9121 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
     9181 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
     9241 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
     9301 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
     9361 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
     9421 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
     9481 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
     9541 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
     9601 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
     9661 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
     9721 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
     9781 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
     9841 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
     9901 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
     9961 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    10021 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    10081 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    10141 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    10201 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    10261 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    10321 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    10381 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    10441 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    10501 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    10561 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    10621 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    10681 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    10741 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    10801 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    10861 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    10921 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    10981 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    11041 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    11101 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    11161 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    11221 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    11281 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    11341 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    11401 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    11461 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    11521 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    11581 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    11641 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    11701 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    11761 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    11821 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    11881 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    11941 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    12001 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    12061 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    12121 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    12181 atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca
    12241 ttaatattcg ataattaaat aattaaacgt ttttctattt ttaagcgtaa tttattc
//
LOCUS       DACEUF010000077        12242 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.080, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000077 DACEUF010000000
VERSION     DACEUF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.080"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            21..170
                     /locus_tag="IXG20_002116"
     CDS             21..170
                     /locus_tag="IXG20_002116"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610301.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            663..1991
                     /locus_tag="IXG20_002117"
     CDS             663..1991
                     /locus_tag="IXG20_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1610302.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2193..3026
                     /locus_tag="IXG20_002118"
     CDS             2193..3026
                     /locus_tag="IXG20_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ1610303.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3042..3779
                     /locus_tag="IXG20_002119"
     CDS             3042..3779
                     /locus_tag="IXG20_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ1610304.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3934..4902)
                     /locus_tag="IXG20_002120"
     CDS             complement(3934..4902)
                     /locus_tag="IXG20_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ1610305.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5134..5967
                     /locus_tag="IXG20_002121"
     CDS             5134..5967
                     /locus_tag="IXG20_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ1610306.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            6001..6840
                     /locus_tag="IXG20_002122"
     CDS             6001..6840
                     /locus_tag="IXG20_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1610307.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6866..7426
                     /locus_tag="IXG20_002123"
     CDS             6866..7426
                     /locus_tag="IXG20_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1610308.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7625..9346
                     /locus_tag="IXG20_002124"
     CDS             7625..9346
                     /locus_tag="IXG20_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ1610309.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9511..10653
                     /locus_tag="IXG20_002125"
     CDS             9511..10653
                     /locus_tag="IXG20_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1610310.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10669..11880
                     /gene="thiI"
                     /locus_tag="IXG20_002126"
     CDS             10669..11880
                     /gene="thiI"
                     /locus_tag="IXG20_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ1610311.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4166 a         2055 c         2546 g         3475 t
ORIGIN      
        1 agctcagttg gtagtagcgc atgactgtta atcatgatgt cgtaggttcg agtcctactg
       61 ccggagtaaa taaagcttat gcaaagaaaa agaaatccaa tcacttgggt ttctttttct
      121 tttattatta tgatgaatca agctttctgt ttttgtcacg tatgttgtaa tctttacgaa
      181 agagtagagt tggctactca taatcaacca tacttcccat catcgctaaa tacgaaataa
      241 atcagcattc ctacaccaat caaagcacaa gctgcaccaa gaaaagggat gacattccca
      301 aagttatctg tattcataag tatgccacca cccacaaaca aaaagccgcc agcaatcagg
      361 tttttcattt tattcatctc ctttcttacg ttatagtatt tccttctttc aatgagtatt
      421 ttgaaagcta tcttattgta tgatgttctt ctaggcctct tctttattat acaggccatt
      481 ttcgttatct catagaaata ttccttttta tctgaaaact attgtatacc gtatgacatt
      541 tttcttcatg aagttgtaag tatattctca ttcaaatgaa aatttcatca tagaagagat
      601 tgtatttcta aattcttaag agtaaaatac gaagtataac ttggatttgg aaggatgatt
      661 ggatgaaagc tattacgcct gatatgacaa caaaatttaa caaacagttt caagcaaata
      721 tcaaacagca ggcattgcag cgggcagttg tgaagaacgg aattactgct tctgctgaaa
      781 atgtttcagc acatgtggag aatacaccgg tattttctat agatttggct acggggaaag
      841 tggctaatca aatgcaatcg gggcgttgct ggatgtttgc tgcactgaac acattccgtc
      901 ataagatgct gaatcaattc catttaaaag agtttgaatt atcacaaaat tacacttttt
      961 tctgggataa atatgaaaag gcaaattatt tctatgaaaa catcattgca acaggaaaag
     1021 aggcaatcga tagccggaaa gtagctttct tactcgctac tcctcaacaa gatggcggcc
     1081 aatgggatat gatcgtttca ttattccaaa aatatggcgt tgtgcctaaa tcggtgatgc
     1141 cggaaagtgc gaatagttca aattctcgag atttgaataa ctacttgaac aaattactgc
     1201 gtaaagatgc agtagtgtta cggaaaatgg ttgctcaagg agaaacatta gacgctattg
     1261 aagaaaagaa agaagaaatg ctagaaagca tttataactt cttagctatc tcattaggaa
     1321 cacctccaaa agaattcgat tttgagtacc gcgatgaaga aaagaactat catcttgacc
     1381 gaggactgac accacaaatt ttttatgata aatacatcgg tgtaaaatta gatgattatg
     1441 ttagcgtgat caatgcacca acaaaagaca aaccattcaa tcgttcttat acagtagaga
     1501 tgctaggaaa tgtcgttggc gggaaagaag tcaaatatct gaatgttgat atggaaactt
     1561 tcaaaaaatt agcgattgct cagcttgaag aaggagaatc tgtttggttt ggttgtgatg
     1621 taggtcaatc ttctactaga actagtggca tcatggcctt agatgcttat tcaatggatg
     1681 atctatttga tacggatttt acaatgacta aagctgaacg tttagatttt ggagaaagtc
     1741 tgatgaccca tgcgatggta ttgaccggtg ttgacttagt agacgggcaa tcaacaaaat
     1801 ggaaagtcga aaatagctgg ggcgaaaaag ttggaaagaa tggcttcttt gttatgagtg
     1861 atgcctggat ggacgaatat acgtaccaaa tcgttgtaag aaaagattta ttgacgaaag
     1921 agcaattaaa tgcgttcaat gaagaaccaa ttgttctatc tccgtgggat ccaatgggtg
     1981 ctttagcata gacgagtatt tcaatgaaga gtatgtagta aagataaaaa gagctggctt
     2041 aagccagctc tttttatctt taaagtccat tccctgtttt ttatggtccc tgtgctataa
     2101 ttctaaataa aggataagga ttggaaataa tccttttatc attaaaaata tgatgacgaa
     2161 gtagaataaa attgggagta ggggggaaag tcatggatat ccaaactgtt ttatctgatc
     2221 tgaaattaaa tggtaaagtg attccagttg ttggtggcga tgttaatcag acctatcgta
     2281 taaaaaccga acatcgtgct tattttttaa aaatccatcc gaatgtaaaa aaaggatttt
     2341 ttgaagcaga agtagacgga ttaaaagaac tgtcagcatt cgtacgtgta ccggatacgt
     2401 atatgctagg tgaaacaagt gaaggcgctt atttgttaat ggaatggatc gagccgggaa
     2461 aaggggatca acgagatctt gctgcagctt tagctaatct ccatcagcaa acagcgccac
     2521 aatttggttt tcgtaaagat aactatttgg gtacacttgt tcagaaaaat tcttttgaag
     2581 aagattggtg gacttttttc tttaaagatc ggcttgaatc ccagatttct ttggcagaag
     2641 aaacgaatcg atggaatgta cagcgtcaag aaaagtatct gcgatttaaa gaacgtgtat
     2701 tgaaatcggt tgaaccaaaa aagatcactc cacgcctctt gcacggagac ctgtggagtg
     2761 gtaatgtgtt ctttgaccag caagggcatc cagtttttgt cgacccagct gtttcttatg
     2821 ggaatcggga acaagatatt gcaatgagcc agttattcgg tggtttccga ccggaatttt
     2881 tagatgctta tcaaactata tttccattag aaaagggatg gaaagaccga ctgccgattt
     2941 accagttata ttatcttttg gcacatttga atatgtttgg agaaagttac ggttcccaag
     3001 tagatcaatt actagaaaat ttttaagagg agggcgtgtt tttggcaaaa aaatatacca
     3061 aaaagcatga agtttcatat tatgagtgtg acatcaacca aacgatgaca tttccttcga
     3121 tgttaggagt agttatcaaa acgtctgaag atcaaagcga ccagttggga agaggcagtg
     3181 aattcgtcaa tagctttgga cttacttggg tcatcacgaa ttattccatg gagattaatc
     3241 gtctccctag agtcggtgaa gtaatctcag taacgactca agccatggag tacaataagt
     3301 ttttctgtta tcggaacttc tggatccatg atgaagatgg gaaagaatta gtcaaaatag
     3361 aatctgtttt cgtattgatg gattttgtga atcgaaaaat gagcagtgtt aatgaagaga
     3421 tcattgctcc ttttgagagt gagaagatca agaaaatcaa gcgtcaggaa aaaattgaaa
     3481 agattgattc gggcttcatg ttgccatatc gtgttcgttt ttatgatatc gatagtaatc
     3541 agcatgttaa taatgcgatg tattttaatt ggatcattga cgtattagga tatgactttt
     3601 tgactacgca tgttccagaa aaagtaatta ttcgatttga taaagaagta gagtacggga
     3661 atgagatcga aagccatttt gaacaaatcc ctatcgaaaa tggtgtcaaa acaaaacatg
     3721 aaatccgaat gcaagaacag ctgtattgtg aagcaaatat tatatggaaa aataaataaa
     3781 aaagaggttg tgacagaagg gggcaacttc aagaaataag aagaaattta cgaaaattgt
     3841 ttctcaaatt tttgtaaatt ttcgacttag ttttcgaaga agctattttt gtcacagcct
     3901 ctctttttaa gtttagctat tagataatga tgtttagttt tggataaacg aaatcaattc
     3961 ttcacccgac aaaccatagt ttacagcaat caatagtttt aaacgtgctt tttgactatt
     4021 aatactgtta cagaaaataa tccccatttt ttctagttct tttcccccgc cttcataatc
     4081 ataaacaggc tctgcgattc cattgaaaca acgagaaaca agtactacag ggattttttg
     4141 gacaagaagc ttttccaaag cagctaaggc ttgtggaggg agattccccg cacctaaagc
     4201 ttcgattacc aaaccgtcta ctttcgtatg ggcgatggct tccaataagt ccccttgcat
     4261 cccagcatat gctttaacga tcgggatagt cccatctaca gcagaaatgt ccagtcgctt
     4321 tgtttctaaa agttcctgca agaataaaat tcgattctta gttactaagc cgatgggacc
     4381 caatgtaggt gttcggaaag tagcaacatt tgtggtatgg gtctttgtca cgtagcgtgc
     4441 agaatggatc tcatcgttca tgaccaccaa aaccccctta tctagcgctt cttcacaact
     4501 tgcgacacga atcgcacttt caaaattata aaggccgtca cttccaagtt cattacttga
     4561 acgcattgcc ccagtcaaaa caattggtaa tttgttgcca atcgttgtat caagaaaaaa
     4621 agcagtttct tctaatgtat ctgtaccgtg tgtgatcacc actccggata tacctgatgc
     4681 ataagcggac aagatacgtt cttttaatac caacatgtga gaaggcatga tgtgtggtga
     4741 gggaagattg aagatatctt caactaataa ttctacatgc ttggggattg tgatttctgc
     4801 atctaataat gggtttgcca catcaggact aacaccgccc gtgagcatgt tttctttcat
     4861 ggcgatcgtt ccgccggtgt gtagtacaag tatttttttc attttacaga cttccttacg
     4921 aataattttg catagccgtt aaatcttatt gtatactata taaaagagtt ttgctatcag
     4981 tgaagaataa cgaatagcct aacttatcat aaaaaagctg accttattaa aatggaaaag
     5041 actcaacctt gacctcctaa tacaatggag tatctggttg agtttctttt acaagaaagc
     5101 agccaaggaa atcaatcacg aaggtggccg gaaatgatca gaatcggatt aacatcgttt
     5161 aaagaacatg acaaattaac tggtaaaaag aataacactc tttatgaata tgcagggttt
     5221 ctacccttag tggaattaga tactgcctat tatggaattc cccgaaagtc ctcagttgag
     5281 aattggataa gtgaagtacc tgaatcattt cgctttgtag tcaaagtcta tcaaggaatc
     5341 agcggtcaag gagactggaa gccttattat cagaccgaag aagagatgat tgcgcatttt
     5401 ttagatgtta tgtcgccaat ggtagatgct ggaaagatgt tttgcttttt agtccaattc
     5461 ccctcatcat tcaaatgtac gaaagaaaat gtccagtact tgcgcaaact tagaaagtgg
     5521 tttgagggtt atcctgtttc tatagaattg cgggattatt cttggtacag cgatgaattt
     5581 cttgaacgta caagaaactt tatgtctaga gccgcttttt ctttagccat catcgatgag
     5641 ccacagttgc tgaatacaac gattcctttt gatccatttg tcaccaatcc gaactttgct
     5701 ctttttcgat tccatggtag gaatgcgcaa ggatggcaag caaatgataa agagtggcgt
     5761 aaaaaaagaa cgttatatcg ttattcagaa aaagaactgc aagaactagc acatgaagtc
     5821 aaagtagttg attctaagac aaaagaggta ggtgtgattt ttaataataa ttctggtggt
     5881 gatgcggctg aaaatgcatt agcactgcaa aaaatattaa atatcaagcc agatggactc
     5941 aatccaaccc aaatggatct gttttaaggg cttattttat tcaagaaagg ggcgaagtct
     6001 atgatcaaag ctattttttt tgatattgac ggaacattgg tcaccaatcg ttcaaaagcg
     6061 ttggaatcaa caaaacaagc aattgaatat gctcggaaaa acggcatttt atgcggtgta
     6121 gcaacggggc gcagcccagt aaagatcaaa gaaatcattg atgaattgga attagatatg
     6181 tatgtcgtgt ataatggtca actggttttt actgcagatc gcacgattat cgaccaccct
     6241 tttgaacaaa aagtattgga acatattgtt gaatttgcag atgagaacca tcgacaaatc
     6301 gtttttggtg cgagaaatcg gttagatgga tcaacgacga tgctattagg acaatcgatt
     6361 tttatcaagc gactagtcag ctttttaccg cgtaaattcc cggtacgtct gatgaaaaaa
     6421 atcctccagg tttttagccc tcaccgacaa aaggatcgtt atgaaacgtt agagatatta
     6481 aaagaaccaa tttatcaatg tatattgctc agtccggagt cagaacagaa aaagttagaa
     6541 catcgctttc ccgagtgtac gtttcagcgt tcaaatacgt atactgtaga tatcatccca
     6601 aaaggtggtt caaaattact aggcatccaa gctttcgcaa atgctgtcga aattgaaatg
     6661 gaagaaatta tggcattcgg cgatcattac aatgatatcg agatgctgaa aggtgtagga
     6721 attggagtgg ccatggggaa tgctcagatc gaagtaaaac atgctgcaga ctttgtgact
     6781 caatcaaatg aagaagatgg gatatacgaa ggactgaaac acttcggtct gatttattag
     6841 tcaatgaaag aaggaaaaaa tcagtatgga agcagagaca ccttttcaaa aagtagaaac
     6901 attccacaaa acttttgatc caagaagacc agagataccc acggctttta cagaaaatga
     6961 agctcataca cgtgctgcgt tcaaaataga agaacttgtt gaatttgttg cagctacttc
     7021 acctaataaa gaacaactgc atgaagctgt tatgctgctg cacgaagcat tagataaagc
     7081 agaagaaaaa atacttggta aaacacagtg gggaagtgat ccgctggttg aacaagtaga
     7141 tgcactgaca gatatccttt actttactta tggttctttt tcattactag gaatagtgcc
     7201 tgatcgaata ttcgaaatcg tccatcaggc aaatatgggg aaattatttc cagatggaaa
     7261 acctcattat gaccctgtaa caaataaagt gttgaaacct gaaaactggc agatggaata
     7321 tgctccagaa cctaaaatcg cggcagaaat ccaacgtcag attcaagaag cgattgctaa
     7381 gaagaaatcc gctgatatag gcatgtgcac aaatgaaaaa gagtgatcag agtttcacta
     7441 tttttgttat ctttatacga tggaaaaaca aaagattttc gtttaagtta gtgaatgcat
     7501 gaaaatagta tgctataatc tagaatagat gtcttttgca cgagagacgt attatttgga
     7561 tttatgccaa gcagaacgcc atctgttgca taaaagatgc atactgaaaa cggaggaacc
     7621 ataaatggga aataatgtta tcatcggaat cgtcattgca atcattatag tcgccattgt
     7681 cgtctttttg gttagttact tgatgaagaa aaaaaaccaa gatcgattgg atgccttaga
     7741 aaaacgcaag gaagaattat ttgacttgcc agtgattgaa gaagtcgatg atgttaaaaa
     7801 aatgcatctg gttgggcaaa gccaaaatac ctttcgtgaa tggagtcaaa aatggaatga
     7861 catatctact tcctcgttcg ctgaactaga aagtcaaatt ttcgaagtgg aaaacctcaa
     7921 tgaaagtttt cggttcttaa aaggaaaaaa agcagttgtt gaagcagaag ctacaatgga
     7981 ccaaatggag aaacaagtga cggaaatccg tgaaggtcta aaggaattgc gggaaacgga
     8041 agaacgcaac tcactagctg ttcagcaggc acttgacgtc tatgaagaac tgaaagaaaa
     8101 agtcagagaa gacgagcatg aatacggacc agctcttttt gaattgcaaa aacagatcaa
     8161 agtgatcgaa aaagaattta cgcagtttgt gactttaaac acttctggag accctgttga
     8221 agcccgcgag gttttggata aagcagaaaa acatacgtat gaagtggaag ctctgatgaa
     8281 aaaaattcca ccgctgtatg aggagctcca taccactttc cctgaacaac ttgaagaaat
     8341 cagtgatact tacgagaaat tgatggaaga acaatatgta ttcccagaag aagatcttgc
     8401 tgaagatatc gcaaaagtaa gtcgaagaat tgaaaattca ttagccaatc ttgaaaaaac
     8461 agaagttgaa acggtcgaat ttgaaaaccg tgaaacagct gatttgattg attcgttata
     8521 tgatatctta gaacgggaaa tggaagctca gagatatgta aaaactaacc aatcaacgat
     8581 tgcagagtat atcaaacata caacaaagaa taaccgtcaa ttattgattg agctggatca
     8641 cacatctcaa agctatacat tgaaccacaa tgaaattggc cgtgtacgag gtttccaaac
     8701 cgaagtggaa gaaatggaac gtcaaaatga acagatgatt ccacagatcc gtcaacacga
     8761 aattccttat tcagaagtgc gcacgttcta taaaacagtc ttcaaagtct tagaagatat
     8821 cgaaaagcaa caagtagaga tcgacgactc attacatgaa ttgagaaaag gcgaaaaaga
     8881 agcgcaagaa aagatcgata cattcgaatt caaattgcgc agtttgaaac gttttgtaga
     8941 aaaacaacgt ttgcctggct tgccgaatga ttacctagaa ttcttttttg ttgctactga
     9001 ccgtattgaa gaactaagtg ttgtcttgaa taaaattcga gtaaacatgg aagaggtcaa
     9061 tcgtttagtt gctctatgtg aagaagatct agaattatta gataagaaga cacatgattt
     9121 agtagatgca gcagcattaa cggagcaaat gctccaatat gcaaatcgtt atcgtcatac
     9181 acacgaagaa gtgagagaag caattgataa aagctactat ttattcaata aagaatatca
     9241 ctatcaagaa gcattagacg aaatcggtac agcattagag cgagtagaac ctggtgcttt
     9301 caaacgcatc gaagattttt attttaatca tccagactta gtataaagca aaaaagttag
     9361 taaaccagca agagcaagac ggattattgt cttgttcttg tctttttttg tatatttgaa
     9421 aattgaaaca agtgaaaatt tcacctgctc ttgaaagcac cttggacaac cgttataata
     9481 gaaaagaatt tcaaaaaaga ggggtttctt atgatatatt ttgataacag tgcaacaacc
     9541 ccaatctttc cgcaaagctt agatgcatat gtaaaaacca gtcagcggat tattgggaat
     9601 ccatcaagct tgcatgattt aggcaatcaa gctaaccggt tgttgcagca agcaagaaag
     9661 cagatcgctg atttgatcca tgtagaacca gaagaaatct attttacttc aggcggaaca
     9721 gaaggagata actgggtatt aaaaggaacg atgatcgaaa aaagagaata tgggaatcat
     9781 atgatcattt caggagtcga acatccggcc gtcagcgaga cagcagaaca attaaaagaa
     9841 ttaggaatcg aagtaagtat cgccccagtg gataaacgcg gatttgtacg tgtcgacgaa
     9901 ttgaaagaat taataagaaa agagacagta ttagtctctg ttatggctgt gaataatgaa
     9961 gtaggatcga ttcagcccat ccaggaaatc agtgatcttt tggatgattt tcctaaaatt
    10021 catttccatg tagatgctgt tcaggcgatt ggaaaagtac cattctcgca atggttgaca
    10081 gaaagagttg attttgcaac attttcagct cataaattcc atggcccacg gggaactggt
    10141 ttcatttatt ggaaaaaggg tcgtcgttta gcaccattac tgacaggtgg agggcaagaa
    10201 agaaatcagc gaagtgggac agaaaatgtt ccaggtattg tggctatggt taaagcttta
    10261 cgtttatcat tagagataaa ggaacaaaaa cctgagcaga cacaacgctt aaaagtagct
    10321 ctaatcaatg cgttgaagac gtatgagaaa gtgacgattt tttcagaagg aactgactat
    10381 gcaccgcata ttttatgttt tgctctaaaa ggaattcgcg gcgaagttct cgtccatgcc
    10441 ttagaagaaa aacaaatatt tgtttctact actagtgcct gctcctcacg taaacatatg
    10501 gcaagcagta cattgcacgc aatgcatgta ccaaatgatc tggctacatc tgctgttcga
    10561 atcagcttag atgaaaacaa ttcgcttgca gaagtcgagc aatttatgat tattttcaag
    10621 cagctttata agaaattttc tgcaatcaat taaaagagga gtgttcttgt gaaatacagt
    10681 gaaattatgg tccgttatgg ggaactttcg acaaaaggaa agaatcgaaa aagctttatc
    10741 atgcaactag cacaaaatgt cagacaagct ttgaaagact ttccagaaat caaaatccat
    10801 gctgatcggg accggatgca tcttttattg aatggcgcag acgggcagct agttattcca
    10861 aaactagcaa aaatatttgg gatccaaaat ttctctccaa gtattcgtgt agaaaaagat
    10921 gtcgaagttt tgaaacaagc tgttcaagac atcatgaaag aaatttatac tgggaaagaa
    10981 acatttaaaa ttatcgctaa aagaagcgat caccaatttg aattgacaag taatgaattg
    11041 aatcaaacat taggtaatgc tgtttttgat attttcccgc atatccaagt tcaaatgcgt
    11101 caaccagata ttccattacg tgtagagatt cggcgtgatg gcgcttatct atcgtatgaa
    11161 acgatcaagg gagctggagg attgcccgta ggaacaagcg gaaaaggaat gcttatgctt
    11221 tctgggggga ttgattctcc tgtagcaggg tactttgcca tgaagcgggg agtggaaatt
    11281 gaagctgttc attttgctag tccgccgtat acaagtgaac aagcactgca aaaagcaaaa
    11341 gatctaacag ccaaactagc accttatgtg ggaacgatcc attttatcga agtgccattt
    11401 accgagattc aagaagaaat caaaaagtca gtgccacaag gatattggat gacaattact
    11461 cgtcgaatga tgttgcgttt gaccgatgaa attcgccgta ttcgtcatgg tttagtcatt
    11521 ttgaatggtg aatcattagg tcaagtggct tcacaaactt tgcaaagtat ggtggctatc
    11581 aatgaagtaa cgaatacgcc aatcattcgg cctgtagcga caatggataa acttgaaatc
    11641 attgaagtag cagaacagat cgatacattt aatctttcta tccagccttt tgaagattgc
    11701 tgtacgattt ttgctcctcc acagccaaaa acacgccctc aactggaaaa ggtccatcaa
    11761 tatgaagagc gtttagcaat agatgaaatg atccaacgag cactagctgg cttgaagatg
    11821 gaagagatac aagcagaatc gtctaatgaa aaaactgaag aattttcaga attgctataa
    11881 gaggttatga aaatagtgtt ttgaccagag tattagagaa acaaaacgaa aaaatagctc
    11941 catgtatttt accgtttttg aactaatgtc gaagattagc tgattgaata ccatttatca
    12001 agaagttatg gaaaagccgg ttctttttta ctaactgaaa aataaaattt tatttagaga
    12061 tcaagctaca gttgataaat gatataaaaa agagaatttc ccatgaaata gcgaggttgt
    12121 gacagaagtg ggcagcttca agaaataaga aggaatttac gaaaattgct tttcaaattt
    12181 ttgtgaaatt tcggcttatt tttcgaagaa gttacttctg gaacaccgtg tatcagaggt
    12241 tg
//
LOCUS       DACEUF010000078        11932 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.081, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000078 DACEUF010000000
VERSION     DACEUF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.081"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            51..260
                     /locus_tag="IXG20_002127"
     CDS             51..260
                     /locus_tag="IXG20_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610312.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="IXG20_002128"
                     /pseudo
     CDS             467..667
                     /locus_tag="IXG20_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1154..1333
                     /locus_tag="IXG20_002129"
     CDS             1154..1333
                     /locus_tag="IXG20_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610313.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1379..1984)
                     /locus_tag="IXG20_002130"
     CDS             complement(1379..1984)
                     /locus_tag="IXG20_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1610314.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="IXG20_002131"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="IXG20_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2666..2893
                     /locus_tag="IXG20_002132"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="IXG20_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="IXG20_002133"
     CDS             3019..3990
                     /locus_tag="IXG20_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1610315.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="IXG20_002134"
     CDS             4130..5443
                     /locus_tag="IXG20_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1610316.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="IXG20_002135"
     CDS             5486..6352
                     /locus_tag="IXG20_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1610317.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="IXG20_002136"
     CDS             6349..7197
                     /locus_tag="IXG20_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1610318.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="IXG20_002137"
     CDS             7211..8683
                     /locus_tag="IXG20_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ1610319.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..9572
                     /locus_tag="IXG20_002138"
     CDS             8700..9572
                     /locus_tag="IXG20_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1610320.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(9808..11190)
                     /locus_tag="IXG20_002139"
                     /pseudo
     CDS             complement(9808..11190)
                     /locus_tag="IXG20_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11652..>11932
                     /locus_tag="IXG20_002140"
     CDS             11652..>11932
                     /locus_tag="IXG20_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610321.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
BASE COUNT         3700 a         2038 c         2402 g         3792 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgcaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aagtggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     9001 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     9061 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     9121 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     9181 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     9241 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     9301 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     9361 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     9421 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     9481 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     9541 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     9601 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     9661 ggtttttgtt ttacttggaa gatcaagcct tgaatgagat aagttcttgc tcgaaagtta
     9721 tggaaatttc gatagccgta ggctacttgt attgtgcaat agttttttac aaaaaaaagg
     9781 gggtgcggat attttttttg gaccttttta acaaatatat ctttgcttct tccgatattg
     9841 aagagggctt agccttccta aagataactt aattcgttga ttattatacc aatgaatata
     9901 atcatccaat ttgtttataa actgattgat tgttgtattc ttccagtcac gttgataaaa
     9961 catctcgttt tttaatcgtc caaagaaacc ttcgcacgct gaattatctg gtgaataact
    10021 ctttttagac atagagtgaa gcaagttggc tttgttccaa tagtccaact aacaatagca
    10081 ccatcgtaac aatcgacaag tggtgataag tagattttcc ctgcgagaat tctaaattca
    10141 gtaatatctg ttaaccattt ttcatttggc ttctttgctt taaaattccg atttaataaa
    10201 ttggggtgtg caggagaaat ttccccagca tacgaagaat acttttttcg tctagaaaat
    10261 ttaacaaata gattttcctc agtcatcaat ctacgtacaa ctttttcaga aagcttttta
    10321 cccatatttt ttaaagcttg gtgaattcta cggtaaccat acgcacagaa gttatgattg
    10381 aaaatcgtga taattaattt gacgttcttt gtaatactta tctggtttct caagtgcttg
    10441 tttttgataa aaatagctgc ttttaggaaa ctggagctgt tgaagaagct ctttcaaggt
    10501 gaactgattt cgtaaggcat caatcaatat cgttttttct tgatttgata gacaaagaag
    10561 attgacgcct ttttcttttt ttagcagttc agccgctcct tcgagtaatg ctttttcgag
    10621 ctgtaattgg tgtacttgtt tttgtaagtc ttctacttcc tttaaaagta gctctttttg
    10681 tttttcctga ttttttaaag tcatacacaa tggaaaatcc tttccaagaa ccgtgttttt
    10741 ccattgatat agagtaactc tactaattcc tccagattcg gctaaatttt tcctattagt
    10801 ttgcggttta tagaattttt tcaacacagc ttctttttga tcttgtgtta actttaccgc
    10861 actttttcgg agttttctag ctcctggtgc aagttcttca caccgctgcc gcaaggactc
    10921 tctattagga taaccaagca tgcgaatggt tcgtgcataa cattgaccat attcaaaata
    10981 atgattcacc gctattcgtt tttgttcttc agaatacttt gatttttttc ggtgctcctg
    11041 atggagctct ccggattcca aatactcttt ataccatttc cgtagtgcac caatcgacgg
    11101 ataccctaat tctcgaatgg ttgcagcata agagcaatcg tattttaaaa ataactgaat
    11161 tgcccttaca cgctctttgt gggaaaacac acggacattc ctcctaagtt tattttttcg
    11221 tccaggatta tgtccgcacc cccttgcttt ctgatgactt attctggttc tttgagtgtc
    11281 aatgcataga tgtgatgtag ctagttagaa aaaaattttg aatactcttt ttttctttca
    11341 gactcctttt taagtaagat tttgaagtgt gagttacggg gtcagaaact taaaagtttt
    11401 tgatccttta ttttcaagtc tgatttggca gcttttcggc agctcattta tttatggtca
    11461 gaaatagatg ctgaaatttt aatctattag caaatagtcc agaacgtatg aagaggagtc
    11521 ggatatcatt tgtcattaac accataaata tttataaaat tcgtgttcac ctacaattaa
    11581 atttaatata taatattcgt tgtaagggct tataattttt tgttaatgaa caagatagga
    11641 agaaacattt aatggacagt tttcagagat tgtcactagt aaatcaattt gagttattgg
    11701 ctaaatttga ggaccaaaac agatcttatt atgaaagaaa aatcgaaata ttgagagaag
    11761 gttacgagta tcattatgat gatgaaattt ggagtgatct ttctgagcca tttccgaaag
    11821 aagattctcg atttgtatta gatgtattga acatgtatcg agatatcaat tttagtaaat
    11881 caaagctaaa cttagatgaa aaccaaatta ctaattcata ctatactcat tt
//
LOCUS       DACEUF010000079        11528 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.082, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000079 DACEUF010000000
VERSION     DACEUF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.082"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(808..1122)
                     /locus_tag="IXG20_002141"
     CDS             complement(808..1122)
                     /locus_tag="IXG20_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610322.1"
                     /translation="MKHPPKTTVEQLAESSGLSDKTIQRMRNGEAVVIQSIVAMCIGL
                     HLHPDISTEMLQKLGYTLGPAVEIHMIYKTLLCNCKTMTIEECNGILTNADFEPLTKA
                     EV"
     gene            complement(1289..4279)
                     /locus_tag="IXG20_002142"
     CDS             complement(1289..4279)
                     /locus_tag="IXG20_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1610323.1"
                     /translation="MKIQFDTLDYQTDAVNSAVRVFEGQTIKESNFTITDDYSRGSMF
                     TEDGIGVGNRVIINEEQMLKNVNKTQILDGIVPSDNLFGNNKAFPQFNIEMETGTGKT
                     FVYLKTILELNKQYGFLKFVIVVPSIAIKEGVLKSFEITKDYFKNQYNGVVYDLFMFD
                     SSKLDRVRSFASANTIDIMVTTIQSFNKDTNVMNRENDQLSGARPIDLIAETRPIVII
                     DEPQSVDNTDLAKDALKSLNPSAGFRYSATHRDNTYPKIYQLGAVDAYDNQLVKQIEV
                     AGIEMDEDGNRAHLKLIDVKTGKNGISAKIEVYKKTRNDADKITMNFKAGDDMFSKTK
                     LQAYDEVGFIQDIDATPGFESVTFSGNPEKITLESATLEDEAIKRAQISKTIEEHLNK
                     ELKFKQSGQRIKVLSLFFLDKVENYRVYDEEGNPSLGRYARIFEEEYERLIHLSKYKE
                     LNDVNVPVSEVHDGYFSADNKGKLKNTKGDTQADESTYEMIMKDKEGLLTFYDEEKHH
                     TAKANKIRFIFSHSALKEGWDNPNVFQIATLIETKDTINKRQKIGRGLRIAVNEDGER
                     VPGFAVNTLTVMANESYKDFAEGLQKEYEEDGVIFGVFNKDSFATIITHYDEVTEEME
                     ILGKEKSEQLFNYLKQEEFINSSGRATDKLKQAITDQEIEVPEEFIDVVPQILVAAKS
                     KVKSLDIKDAKDKVEVKVVKEALADDFLELWNKIKYKTTYKIHFDSNKLIEFAAKGNE
                     SFDGVDSIQTRKGTYLYKKGMVEMSQAGVKADESGESSVRATTSGMYKLPDIITFLQN
                     ETSLTRKTIVAILQSVNNLDSFKNNPLSYMNQAAKIINAIKNQLIVDGIEYTRTNDEY
                     EQTLFSTETLNAYLGEKGNSVKVDESKQKTVYDYVVTDSNIEKDFARKAEMDDNVKFY
                     IKLPDWFKIRTPLGPYNPDWALLYEEDDEQHLYFIVETKGDTSFEQLRPHERAKILAG
                     EKHFKAVDTDIKFKRVSDEAEIRG"
     gene            complement(4300..6144)
                     /locus_tag="IXG20_002143"
     CDS             complement(4300..6144)
                     /locus_tag="IXG20_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HAQ1610324.1"
                     /translation="MINENNQVPSSSLDITEQNIEKLKELFPEVLTEKKIDFDKLRLI
                     LGDEVETAPERYSFTWNGKKQAMQLAQQPTVATLKPNKAKSKNWDETQNLYIEGDNLE
                     VLKILQKSYANKVKLIYLDPPYNTGSDFVYKDSFSDSLENYLEVTCQVDDEGKKFSTN
                     SDTDGRYHTNWLNMMYPRLKLARNLLRQDGVIAISIDDNEVAKLRNLLDETFGENNFI
                     QEIAWRRSDNQANIGLAARVKEYILIYAKNKKELVYGRLPLSESAIREYRYSDSYAGK
                     FRRGILLDKTRGRYSYPVTTKSGNVLNGPWMVAEEKFKELDENNGIYWTSGGEEQPYS
                     KIYLSESKGAIPNDFWGIEYGTNQRASLEVEVLFGERYFDFPKPVSLIKSLISLGAPD
                     KDDIVLDFFSGSGTTAQAVMESNIDDDGKRKFILVQLPEDIDAKHKAYKAGMRKIPEI
                     AEKRISLAGDKIIAENPELAGKLDVGFKVFELSKSNIKKWNAEPKDLNEQFELLANNF
                     EEGSKPIDVVYEIMLKQGLDLTYPISETKVGEAVVYDIAFGAMFVVLGDKITSEVASH
                     IIKQIADEEAENSVVVLQDEKFINDSEKLNTIEQLNASGIQYNDILSI"
     gene            complement(6172..6894)
                     /locus_tag="IXG20_002144"
     CDS             complement(6172..6894)
                     /locus_tag="IXG20_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4391 domain-containing protein"
                     /protein_id="HAQ1610325.1"
                     /translation="MSELETKDIIKWWKFPSATIINRNLPKTQIYPHMKNAADKQFLQ
                     DSVQSIYMLASLKTDNTHIAVYEDDKELYQEIQFLYVKTKDKGDSTKIYKMLAHLIPY
                     PLVILTDEPDSFTIYTGRFEKLSTGFLKLMNVYPSPVYQDENLEEVLQQISLIDLPRQ
                     NLKTFYDGLRDEIVTATAKSQYGEAVANITGEEKDQLDALKKQIEDLRGQIKKERQLN
                     RKIDMQMKLKKLKDELSSKINQ"
     gene            complement(6895..10152)
                     /locus_tag="IXG20_002145"
     CDS             complement(6895..10152)
                     /locus_tag="IXG20_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002403337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1610326.1"
                     /translation="MELKLIDNKGDNTLENLISEKVTKHSKISIQTAAFSIFAFHALQ
                     KEIKRSKGLRLVLTKSFFEKEETSFLKRYEIAQSEEDISGNKYEIKLRNKMNTAFIAR
                     ETARLIEEKVKVHQLGPNQNALDELLIENEADIEGNVVVPSMFKFTSDGLGLTESNNV
                     SSMTGIIGEPNHLAGLKADFDAVWNDPKKSKEVTDKVLEKVRTIYQENPPEWIYFVSL
                     YHIFHDKLSELDEQDIAPEGSGFKDSVIWNKLYQFQKDGVMGLIRKLEKYNGAILADS
                     VGLGKTFSALAVIKYFELQRKRVLVLAPKRLRDNWTLYTRPDVRNVLVDDYFNYTVLN
                     HTDLSRYKGESDGIKLDTFLWSDFDLVVIDESHNFRNNDTSVNRETKTRYQRLMEDII
                     QKGRKTKVLMLSATPINNRMNDLKNQIGFITEGKTDALAKYDVDDIDTTLRLAQQAFN
                     QWMELDESERTTQSFLDKVNPDYFKLLDMLTIARSRKHIEKYYDTTSLGDFPERLKPM
                     TVKPNIDSSGQFPNLHDVYDSIGYLNMSMYQPLQYVLSTKRKRYEELYDTKVKEGKSS
                     FRQVDREQSLAKLIRANLFKRLESSIYSFGSTLDRMVGGIEDILEKIKEHQAGNVEMP
                     SITDFDDEDLESAFDEQAVGTKTKILIGDMDLVKWSQALNEDLEKLKELQTVTHRVTP
                     DKDDKLMDLEKILKHKYEHPINGNNKKVIIFTAFADTANYLYENLADKVKRLYGLNSA
                     LVTGGSTPNKTTMKGVAVNDMSDILTNFSPISKGRDQINPNATEEIDLLIATDTISEG
                     QNLQDADYLINYDIHWNPVRVIQRFGRIDRIGSRNKQIQLVNFWPNVDLDTYLNLEQR
                     VKGRMVMLNTAATGEDDLLNIKSNKEMNDLKYRKKQLEQLQTQVIDLEDVNGAISITD
                     MTFNDFKADLQNALKEHEKLLNEAPLGMYAITDTTNFPEAEPGVILTLKQQTNDLGQE
                     NSILPYIVVYMRMDETVKINYMYAKQVLDFFKKLTVGKKTVNQPLVDEFYEETNGGKD
                     MSAYSEILSKAIEAIRGKQDEVGMGSLFSPGGTSVQTELLDDLDDVELISFLIIR"
     gene            complement(10201..10401)
                     /locus_tag="IXG20_002146"
     CDS             complement(10201..10401)
                     /locus_tag="IXG20_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002403339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1610327.1"
                     /translation="MMSYKKLWKLLIDRDMSKKDLAELADLSQYTINKLNNSGTVTTE
                     TLTKICIALNCDVGDICEVVAA"
     gene            11367..>11528
                     /locus_tag="IXG20_002147"
     CDS             11367..>11528
                     /locus_tag="IXG20_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1610328.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVSIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEY"
BASE COUNT         3346 a         2416 c         1819 g         3947 t
ORIGIN      
        1 aagtaaatct atttttttaa acgtagattc acaatttcat taaatttttg taatgtttcc
       61 tcatcaggta aaataacttc tagttcgatt atcatccctt tcgttaaata cttaataatt
      121 gaccctgaag atctagaagt aattttattt aaatattagt aatcaaacat tttatttgag
      181 ttcgattcta ttatataatt tcgatgataa gcttcaaatt taccctcgaa tctattaaac
      241 caacactctc catttccaac cagtatcatt gcctttgtat caaaagagta tgatggatat
      301 tttagatgtt ctgagctaaa aatgtaacat ggatattgac catcttaggt agcgtgatta
      361 gcatcttttt tgccggttgt tattgttgtt aaatccccta atttttttcg attccattct
      421 ttcggtagga cttgtttatt tttagaagct agttgtccat cttcactagt taaattggaa
      481 tctacaaacc atttatgaaa tgtcgaaagg tatagaaaat aagcattttt actggggtta
      541 accagcagta atgttctatt tctattcctt gcccttattg caaatcgggc tctgaatatt
      601 aatttttatc gtgactaatt ccttagtact cccttatcat atctagattg agcgctgtaa
      661 accatgacac ttactgtccg ttaatttcgt ctaaaacacg caaaaaaaga cctgattcaa
      721 agatataaaa tctctaaatc aggtcttcat tgagtttaca aaccggacag taagtgtctg
      781 attctcataa aaataaattt aaaatcttta aacttcagct ttggttaacg gttcgaagtc
      841 agcattagtc aaaattccgt tgcattcttc aattgtcatc gttttacaat tgcaaagcag
      901 cgttttataa atcatatgaa tttctaccgc tggccccaac gtatatccga gcttttgaag
      961 catttctgta ctaatatcag gatgaaggtg caaaccaata cacattgcga caattgattg
     1021 aataactaca gcttctccat ttcgcattcg ctgtattgtt ttatcagaaa ggccagatga
     1081 ttctgccagc tgttcaacgg ttgtctttgg tggatgcttc atcaacacct taagcgcatc
     1141 accgaagcta agtggcgcaa tttcattcta tacttgtttt aaaccttttt gatctttctt
     1201 caattaaact gatgcatatt aacatagttt ttaaattaac gttgctgtac cattgattta
     1261 cttctccctg ttttatttca aatatagatt acccacgaat ctccgcttca tcagaaaccc
     1321 gcttaaattt aatatctgta tcaactgctt taaagtgttt ctcaccagcc agaatcttag
     1381 cccgttcatg aggacgtaac tgttcaaaac ttgtatcacc tttcgtttca acgatgaagt
     1441 ataggtgttg ctcatcatct tcttcatata gcaatgccca gtctggattg taaggcccaa
     1501 gcggtgtgcg aatcttgaac caatctggca gcttaatgta gaacttaaca ttgtcatcca
     1561 tttcagcctt acgagcaaaa tccttttcaa tgtttgaatc agttacaaca tagtcataaa
     1621 cagtcttttg tttactttca tcaactttaa ccgaattccc tttttcaccg aggtaagcat
     1681 tcaaggtttc agtgctaaac agcgtctgtt catattcatc atttgtacga gtatattcaa
     1741 taccatccac aatcaattga ttcttgattg cgttgataat cttcgcagcc tgattcatat
     1801 atgacaatgg attgttctta aaactatcca gattgttgac tgactgcaaa attgcgacga
     1861 ttgtcttgcg agtcaaactt gtttcatttt gcaagaaagt aatgatatct ggcaatttgt
     1921 acataccact cgttgttgca cgaacactgc tttcgccaga ttcatcagcc tttacaccgg
     1981 cttgactcat ctcaaccatc cccttcttgt aaaggtaggt ccccttgcga gtctgaatgg
     2041 aatcaactcc gtcaaatgat tcatttcctt ttgcagcaaa ttcgattaat ttgtttgaat
     2101 caaaatgaat cttataagtc gtcttgtatt tgattttgtt ccaaagttct aaaaagtcat
     2161 ctgctaaggc ttcttttaca accttgactt cgactttatc cttggcatct ttgatatcaa
     2221 gactcttaac cttagattta gcagcaacca aaatctgtgg cacaacatca ataaattctt
     2281 ccggtacttc aatttcctga tcagtaatcg cttgtttcaa cttatcggtt gctcgcccag
     2341 atgaattaat aaattcttcc tgtttgaggt agttgaacaa ttgctcagat ttttctttgc
     2401 caagaatttc catttcttcc gtaacttcat cgtaatgcgt aataatcgtc gcaaagctat
     2461 ctttattaaa tacaccaaag ataacaccat cttcttcgta ttctttttga agaccttcag
     2521 caaaatcctt atagctttca tttgccataa cagtcagcgt gttaacagca aatccaggga
     2581 cacgttcacc atcttcgtta accgcaatac gcaggccacg accaattttc tgacgtttgt
     2641 taattgtatc ctttgtttca atcaaagtgg caatctggaa tacattcggg ttgtcccaac
     2701 cttccttcaa agctgaatga gagaaaatga agcgaatttt attagccttc gctgtatgat
     2761 gcttctcttc atcatagaaa gtcagtaagc cttccttgtc cttcataatc atctcgtagg
     2821 tagattcatc ggcctgagta tcacccttgg tattttttaa cttaccttta ttatctgctg
     2881 agaaataacc atcatggact tcggatactg gcacattcac atcgttcaat tctttgtact
     2941 tggacaaatg aatcaagcgt tcatattctt cttcaaaaat acgagcataa cggccaagac
     3001 taggattacc ctcttcatca taaacgcggt agttttcaac cttatccaag aagaacaaac
     3061 tcaacacctt gattcgttgc cccgattgtt tgaacttcaa ctccttgttc aagtgttctt
     3121 caatcgtctt agaaatctga gcacgtttta ttgcttcatc ttctaatgta gctgactcaa
     3181 gcgtgatttt ttcaggatta cccgagaaag taactgattc aaatccaggt gtagcatcaa
     3241 tgtcttgaat gaacccaact tcatcatacg cctgaagctt ggttttacta aacatatcgt
     3301 caccggcttt aaagttcata gttattttat cagcgtcatt acgtgtcttt ttataaactt
     3361 caatcttggc actgataccg ttcttaccag tttttacgtc aatcaatttc aaatgagcac
     3421 ggttaccatc ttcgtccatc tcgatgccgg ctacctcaat ctgcttaacg agttgattgt
     3481 cgtaagcatc aaccgcacca agttgataaa tcttcggata agtattatct cggtgagtgg
     3541 ctgaataacg gaagcccgct gacggattta aactcttcaa agcatcttta gccaagtcag
     3601 tattatcaac agactgtggt tcatcaataa taactatcgg ccgtgtttca gcaatcaggt
     3661 caattggtcg tgcaccagaa agttggtcat tttcacggtt catgacgttt gtatctttgt
     3721 tgaaagattg aatggtcgta accatgatat caatcgtatt tgccgatgca aatgaacgaa
     3781 cacggtcaag tttactgcta tcaaacatga ataaatcata aaccacaccg ttgtattgat
     3841 ttttgaaata gtcttttgta atctcgaagg atttaagtac cccttcctta atcgcaatac
     3901 ttggcactac aatcacgaat ttcaaaaatc cgtattgttt gtttaattca agaatcgttt
     3961 tcaagtaaac aaaagtctta ccagtccctg tttccatctc aatattgaac tgcgggaaag
     4021 ctttgttatt tccaaacaga ttgtcacttg gcacgattcc atccagaatt tgagttttgt
     4081 tgacgttctt caacatctgt tcttcattaa taatcacacg gttaccaaca ccgattccat
     4141 cctcagtgaa catcgaacct cgtgaataat catctgtaat cgtaaaattt gattctttaa
     4201 ttgtctgacc ttcaaacacg cgcaccgcac tgttaacggc atctgtttga tagtcgagtg
     4261 tatcaaattg tattttcaag agtctttacc tcctttgctt taaatactta aaatgtcatt
     4321 gtactggatg ccacttgcat tgagctgctc aatcgtattt aacttctcac tatcattgat
     4381 gaatttctca tcctgtaaaa caactactga gttttcagct tcttcatcag caatttgctt
     4441 aataatatga cttgccactt cgctcgtaat cttgtcacca agtactacaa acattgcacc
     4501 aaaggcaata tcataaacaa ccgcttcccc aacttttgtt tcactaattg gataagtcaa
     4561 atcaagacct tgcttcaaca tgatttcata aacaacatcg attggttttg aaccttcttc
     4621 aaagttgtta gccagtaatt caaactgctc attcaagtct ttgggttctg cgttccattt
     4681 cttgatattt gatttagaga gttcaaaaac tttgaaacca acgtcaagtt ttcctgctag
     4741 ttcaggattt tcagcaatta tcttgtcccc ggctaaagag atccgttttt ctgcaatttc
     4801 tggtattttc cgcattcctg ccttgtatgc tttatgtttt gcgtcaatat cttccggcaa
     4861 ttgaacgaga ataaactttc tttttccatc atcatcaata ttggattcca ttaccgcttg
     4921 tgctgtagtt ccagagccag aaaagaagtc taggacgata tcatctttat ctggtgcacc
     4981 aagtgaaatc aaacttttga ttaatgaaac aggctttgga aagtcaaagt atctttctcc
     5041 aaataaaacc tctacttcta aactagctcg ttgattcgtt ccatattcaa ttccccaaaa
     5101 gtcattcggt attgcacctt tactttcaga caagtaaatt ttgctatatg gttgttcttc
     5161 accaccgctt gtccagtaaa tgccattatt ttcatctagt tctttaaatt tttcttccgc
     5221 aaccatccaa ggaccattta aaacatttcc agattttgtt gttactggat aagagtagcg
     5281 tccgcgagtt ttatccagta aaattcctcg tcggaacttt cctgcataag aatcagagta
     5341 gcgatactcc cgaatcgcac tttctgataa aggcaatcgt ccataaacta attccttctt
     5401 attttttgca tagattaaaa tatactcttt cactcgtgca gctaaaccaa tattagcctg
     5461 attatcagag cgtcgccatg caatctcttg aataaagtta ttttcaccaa aagtttcgtc
     5521 aagtaaattt ctaagctttg caacttcatt atcatcaata gaaattgcaa taactccatc
     5581 ttgtcttaaa agatttctag ctaacttaag acgtggatac atcatattca accagttagt
     5641 atgatatctt ccatctgtat ctgaattagt tgaaaacttt ttcccttcgt catcaacctg
     5701 acaagtaact tctaaataat tctcaagcga atcagaaaaa ctatccttat acacaaaatc
     5761 actacccgtg ttgtagggtg gatccaaata tatgagtttc actttattgg cataagactt
     5821 ttgcaaaatc ttcaacacct caagattatc tccttcgata taaagatttt gtgtttcatc
     5881 ccagttcttt gactttgcct tatttggctt caatgttgcc accgttggct gttgagccag
     5941 ctgcatcgct tgtttcttgc cgttccaagt aaaactgtag cgttcgggtg ctgtttcaac
     6001 ttcatcccca agaatcaaac gtaacttatc aaagtcgatt ttcttttcag ttaatacctc
     6061 aggaaataat tccttcagct tttcaatatt ttgttcagtg atatcaagag aacttgaagg
     6121 cacttggtta ttctcattta tcataaatag gatttccttt cacactttca cttactgatt
     6181 aatcttgctt gaaagctcat cttttaactt tttcagcttc atctgcatat caattttgcg
     6241 attcagttga cgttcctttt taatttggcc acgcaaatct tcaatctgtt tctttaatgc
     6301 atcaagctgg tctttttctt caccagtgat attagcaact gcttcaccat attgtgactt
     6361 agccgtagct gtcacaatct catcccgaag tccatcataa aaagttttca gattctgccg
     6421 tggtaaatca atcagtgata tttgctgcaa cacttcttcc aagttttcat cttgataaac
     6481 cggggatgga tacacattca tcaacttcaa aaagcctgtt gataacttct caaagcgacc
     6541 cgtgtagatc gtaaaactat ccggttcatc ggtgaggata actaatggat atggaatcag
     6601 gtgtgccaac attttataga ttttagtcga gtcaccttta tccttggttt tcacataaag
     6661 aaactgaatt tcttgataaa gttccttgtc gtcctcatac acagcaatat gtgtattgtc
     6721 agttttcaga cttgccaaca tataaatgct ttggacggaa tcttgtaaga attgcttgtc
     6781 cgccgcattt ttcatatgtg gatagatttg cgtttttggt agatttcgat tgatgattgt
     6841 agcacttgga aatttccacc atttgattat gtctttcgtt tccaattcgc tcacctacct
     6901 aataatcaaa aatgaaatta actcaacatc atctaaatca tccaatagtt cagtttggac
     6961 gctagtacca ccaggtgaaa acaatgaacc catccctact tcatcttgct tacctcgaat
     7021 tgcttcaatc gctttactca agatttcaga ataggcactc atgtccttac caccattcgt
     7081 ttcctcataa aactcatcta caagtggttg gttgaccgtc tttttaccga ccgtcagctt
     7141 tttaaagaag tccaacactt gcttggcgta catgtagttt attttgactg tttcatccat
     7201 tcgcatataa acaacaatgt acggcaagat actgttttct tgtcccaagt cattcgtctg
     7261 ctgcttcaag gtcaatatga cacccggttc agcttcaggg aagttggttg tatccgtaat
     7321 tgcatacatg cctagcggcg cttcattcaa caacttctca tgctctttca aagcattctg
     7381 caagtctgct ttgaagtcat tgaaggtcat gtcggtaatg gaaattgccc cattgacgtc
     7441 ttccaaatca atgacttgag tttggagttg ttccaactgt ttcttacggt atttcaagtc
     7501 gttcatttct ttattagatt taatattcaa taagtcatct tcaccggttg ctgccgtatt
     7561 taacatgacc atacgtccct tgacacgttg ttccagattc agataagtat ccaagtccac
     7621 attcggccag aagtttacta gttgaatctg tttattacgt gagccaatac ggtcaatacg
     7681 cccgaaacgt tgaatgacac gcactggatt ccagtgaata tcgtagttaa tcaagtaatc
     7741 tgcatcctgc aagttttgcc cttcagagat ggtatccgtt gcaatcaaca aatcaatttc
     7801 ttcggttgcg ttcggattga tctggtcacg cccttttgat atcggtgaga agttcgtcaa
     7861 gatatcactc atatcattga cggccacacc tttcatggtt gttttgttcg gtgtagaacc
     7921 acccgtcacc aatgcagaat tcagaccata aagtcttttt actttgtcag ctaagttctc
     7981 atagagatag ttagccgtat ctgcaaaggc agtaaagatg attactttct tgttgttgcc
     8041 attaattgga tgctcatact tatgcttgag aattttctcc aagtccatca acttatcatc
     8101 tttgtccggt gttactcgat gcgtgacggt ttgcaattct ttcaactttt caagatcttc
     8161 attaagagct tgggaccact tcaccaagtc catatcacca atcaatattt tagtttttgt
     8221 ccccactgcc tgttcgtcaa aggcactttc caaatcctca tcatcaaaat cagtgatact
     8281 tggcatttcc acgttacccg cttgatgctc tttgattttt tcaaggatat cttcaatccc
     8341 gccaaccatt cggtcaaggg ttgatccaaa cgaataaatg gaactttcca atcgtttgaa
     8401 caaattggca cgaatcaatt tagccaagga ttgttcacgg tcaacttgcc tgaatgacga
     8461 cttcccttct tttaccttgg tgtcatacaa ttcttcgtag cgctttcttt tagtcgagag
     8521 cacatactga agtggttggt acattgacat gttcaagtaa ccgattgaat cataaacatc
     8581 atgtaagtta gggaactgcc ctgatgaatc aatatttggt ttcactgtca ttggcttcag
     8641 gcgttcaggg aaatctccca atgaagtcgt atcataatat ttttcaatgt gtttccgact
     8701 acgagcaatc gtcaacatat ccagtaactt gaaataatcc ggattgacct tatcaagaaa
     8761 gctttgagtc gtccgttcag attcatccag ttccatccat tgattaaaag cttgttgtgc
     8821 taaacgcagt gtcgtgtcaa tatcatcaac atcatacttc gccaacgcgt ccgttttgcc
     8881 ttcagtgatg aaaccaatct gatttttcaa gtcattcatt cggttgttga ttggtgtagc
     8941 tgaaagcatc aatacttttg tcttacgtcc cttttgaatg atatcttcca tcaagcgttg
     9001 ataacgtgtt ttggtttcac gattgacgct ggtatcatta ttacggaagt tgtgggattc
     9061 atcaatgaca accaaatcaa aatcagacca cagaaaagta tccagcttga ttccgtcact
     9121 ttcaccttta taacggctaa ggtcagtgtg gttcaaaaca gtgtagttaa agtagtcatc
     9181 cactaaaaca tttcttacat caggtcgtgt ataaagtgtc cagttatccc gtagtcgttt
     9241 tggagcaaga acgagtactc gtttacgttg caactcaaaa tatttgataa cggccaaggc
     9301 tgagaaagtt ttacctaaac caactgaatc tgctagaatc gcaccgttgt atttttcaag
     9361 cttacgaatt aagcccatca caccatcttt ttgaaattgg taaagcttat tccaaattac
     9421 tgaatccttg aacccagaac cttctggcgc aatatcttgc tcatccaatt cactcaactt
     9481 gtcatggaaa atatggtaaa gcgatacaaa ataaatccat tcaggtggat tttcttgata
     9541 aatggttctg actttttcaa gcaccttgtc tgttacttct tttgactttt tagggtcatt
     9601 ccagacagcg tcaaagtcag cttttaaacc agctaagtga ttaggttcac caataattcc
     9661 cgtcattgaa gaaacgttat ttgactctgt aagtcctaag ccatctgaag taaatttgaa
     9721 cattgaagga actaccacat taccttcaat atctgcttca ttttcaatca gtagttcatc
     9781 caaagcattt tgatttggtc ccagctgatg aacctttact ttttcctcaa tcaaccttgc
     9841 cgtttcacga gcaataaaag ccgtattcat tttatttctg agcttaattt catacttatt
     9901 tccagaaata tcttcttcac tttgcgcaat ttcataccgc tttaaaaaac tagtttcttc
     9961 cttctcaaag aaagatttcg tcagaaccaa acgcaacccc tttgaacgct tgatttcctt
    10021 ttgtaatgcg tgaaaagcaa aaattgaaaa tgcagcagtt tgaatactga ttttcgaatg
    10081 ttttgtcacc ttttcactga ttaaattttc tagggtgtta tcccccttgt tatcaattaa
    10141 ttttaattcc atgagcgtct cccacaaggc tataatttta attctttatt ctgtgctgta
    10201 ttaagcagct accacttcac aaatatcccc aacatcacaa ttcagtgcga tgcaaatttt
    10261 agttagtgtc tcagttgtca ctgtgccact attatttaat ttgttgatgg tgtattgact
    10321 aagatcagcc agttcagcta aatctttctt tgacatgtct cggtcaatta ataacttcca
    10381 caatttttta tagctcatca tcttatcacc tattttctct acaatctgat atgccgataa
    10441 taaatatatt atatcacgat tgatatgaaa ttttacataa aaatatagcg aacacttaat
    10501 tattttacga ttgattagat tttgataaat atttatagga acgtaaattt tagggggtgt
    10561 ttaggggtgt catagggtgt taattcaaaa cctaggggtg ttatattttt atccccttgg
    10621 aaattatgcc tgtatctcat tgatgaaatt catcgtaaaa taaaaaagtt tctcaagcca
    10681 caagctcaag aactcccgta ttatcgcctt ttctagcagt ttgtatttgt atcgattgcg
    10741 tggttttaat atcaatgtaa attagcttta cctttggaaa gcattaatag aaatgactga
    10801 agctagtaag gaactggcag ctaagttaac tgaaaatagt tatttagctt atattaatga
    10861 aaaatccaca cgagaattat ggacactcga tgactaacaa tatccttatt agtatctatc
    10921 cacgatacat ggaaaaaatt ataaacaaac aaaaaaactt tgaatttaaa atttctatct
    10981 tacctcagct ccttataata tcttttggat ttacgaaaca atcccaccaa agctttaaag
    11041 tataaattga ttgtaaaaaa tccaattact aaacttaaga aaaatcaaca ctacttttta
    11101 ggagacgatg agtttgaaca attagtttaa aagggaaaat atgcttacga aattatccac
    11161 ctagaagaaa tacttttacc aataagtctg tctaatttaa aaaatttagg agttactgca
    11221 ccacaaggat atgcatatat aaaaaaatat cctagtcttg tcgatattct cgccgtttgt
    11281 ataattaagc gagacaaata aaaaagccat ttatgttaca ctcaaattgt aatacccgct
    11341 aaagcataca aggagagtga acataaatga cttataccca tcttacacca aacgagcttg
    11401 taatgataga agcatatttt catcaagaaa ctccggtttc tatcgttgcg aagcagctta
    11461 aacgtggacg ccaaacaatt tacaatgtct ataactttct caaatgtggt ggaacagcac
    11521 ttgaatac
//
LOCUS       DACEUF010000080        11346 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.084, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000080 DACEUF010000000
VERSION     DACEUF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.084"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..936
                     /locus_tag="IXG20_002148"
     CDS             <1..936
                     /locus_tag="IXG20_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1610329.1"
                     /translation="GRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESL
                     AIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADH
                     IESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVA
                     MLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFV
                     TQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQC
                     LISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3122
                     /locus_tag="IXG20_002149"
     CDS             939..3122
                     /locus_tag="IXG20_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1610330.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3119..4120
                     /locus_tag="IXG20_002150"
     CDS             3119..4120
                     /locus_tag="IXG20_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1610331.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4135..5049
                     /locus_tag="IXG20_002151"
     CDS             4135..5049
                     /locus_tag="IXG20_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1610332.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5294..5410
                     /locus_tag="IXG20_002152"
     CDS             5294..5410
                     /locus_tag="IXG20_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1610333.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5426..7345
                     /gene="tet(M)"
                     /locus_tag="IXG20_002153"
     CDS             5426..7345
                     /gene="tet(M)"
                     /locus_tag="IXG20_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1610334.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7443..8816
                     /gene="tet(L)"
                     /locus_tag="IXG20_002154"
     CDS             7443..8816
                     /gene="tet(L)"
                     /locus_tag="IXG20_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ1610335.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9380..10642
                     /locus_tag="IXG20_002155"
     CDS             9380..10642
                     /locus_tag="IXG20_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ1610336.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10887..>11346
                     /locus_tag="IXG20_002156"
     CDS             10887..>11346
                     /locus_tag="IXG20_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ1610337.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINK"
BASE COUNT         3740 a         1897 c         2376 g         3333 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt caatctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtaa ttatgaaaaa taccaaagat
      121 tctgaatcac tggctattga tattttgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtgcgaggg
      241 ttgatgaaag tgattgaaga attacgggtt gagaatacgc cactaagtac cagcatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcccatc tgctttttag tgatggttat
      361 gtagagcagt ctatcagcct tgaaaaacaa ctgaacatta tacaggttgc cgacttggta
      421 cttcctgaca aggaaacttc ctttgaggaa tataccacaa tggagttact atcggtagca
      481 atgctgattg tcattagtac ctttgcgttg gactttatcc atacagaccg aagtattttc
      541 aagattgtag acttagacga agcatggagc ttcttacagg tggcacaagg gaaaacacta
      601 tctatgaagc tggttcgtgc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagatg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgccttt
      721 cgttccactg accttaacga gattaaaaaa acccttgcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atattaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggctacc gtggaactgg tcggacggaa
     1201 ttggaaaaca agtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtc
     1261 tttatgtttc caatgcgacg ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggcaagaata tgcagaccct tgcaggggtt acgcctagtg
     1381 gactatcaac agagggtttc tatgttggat tccttttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacagga ctgataaaga gagaaaccac aaaggcaatt catgccctca
     1501 tgaattttgt gctggttttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagcaacgc tagtttgtca ctgggtacaa
     1621 aaattgtcat gccccattca gatagtcaag gcaaggacag tgtggacttg atacgtgaca
     1681 gcctattttc catacaggtt cagcaaccgt ggctactgct tcaatataac agttcagaca
     1741 ttgaaagtat tggtattgac cgtgtggaaa gcctgctctc aaccagtcca gattccaaca
     1801 atggggaaga cagagaaaaa attgttgcgg aagaaattga agatagaagc aataccaata
     1861 tgacgattac aaagacgatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttta
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtatgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ctccgtgact gctttagggg ctgggtctgc cattgccagt gctacgggta
     2401 aaaaaggaca gtcgggttcg ggaagttctg cgagtacaca agcagatcac tctcgaccaa
     2461 acgggcagga aaaatcaaca ctaggaaagc gtatcggtca aaccatcggt acagtagctg
     2521 ataccaaaga cagaatggta gatactgctg gtaatttgaa agaacaggtt aaggatttgc
     2581 cgaccaatgc aagatatgca gtatatcaag gaaaatctaa agtgacggat aatgtccatg
     2641 atttaaccag aagtatttct caaaccaaag cggataaagc cagtggacgc aagaaacagc
     2701 aggaacaaag acggaaaacc attgcggagc gtcgctctga aatggaacag gtcaaacaga
     2761 aaaaacagcc agcttcttcc gttcatgaaa gaccagccac taagcaagaa caatctcatg
     2821 atggacagac ttcaaggcaa acaactgtac aggcttccta tagggaatct caacaagcca
     2881 aacaagagcg tccaacagtt aagtccgatt cttcaagttt aaaaacggaa cgtcaaagta
     2941 atacagttca agaaagaacg gttcaaaagc cagtaacttc aactgcacca acagatagag
     3001 cttcacaacg ttcaatcaca aaagaacgtc agtctactgt tcaaagagta ccactacaaa
     3061 atacaaaaag tagaccacca atcaaaaccg ccaccattaa gaaaggcagt aagaaaccat
     3121 gaagctgaaa actttagtga ttggtggttc gggattattc ttgatggtct tctcactgct
     3181 tctgtttgtc gccattttat tttcagatga acaagatggc ggtttttcca acattcacta
     3241 tggcggtgtg gatgtttccg cagaagtgct ggctcataag cctatggtag aaaaagttgc
     3301 caaagaatat ggaattgaag aatacgtcaa tatacttctt gcgattatac aggtggaatc
     3361 gggtggtaca gcagaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     3421 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgagt tattagccag
     3481 tagcgaaagg cttggtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     3541 tttcttagga tatgtggcta atcgtgggaa taaatatacc tttgaactgg ctcaaagttt
     3601 ctcaaaagag tattcgggtg acgaaaaagt gtcttacccc aatcccatag ccatacctat
     3661 caatgggggc tggcgataca attacgggaa tatgttttat gtgcaactgg taacgcagta
     3721 tcttgtcaca acagagtttg atgatgatac agtacaagcc atcatggacg aagctctgaa
     3781 atttgaaggc tggaagtatg tctatggcgg agcttccccg actacctctt ttgattgtag
     3841 cggactgaca caatggacgt atggaaaagc tggaatcaac ttaccacgaa cagcacaaca
     3901 gcaatatgat gtaacccggc atatcccatt attggaagca aaagccggcg atttggtttt
     3961 ctttcattct acctataacg ctggctctta tattactcat atcgggattt atttaggcga
     4021 taaccgtatg tttcatgcag gcgaccctat cggttatgcc gacttaacaa gctcttactg
     4081 gcaacagcat ttagtgggag caggacgaat caaacaataa gaaaggacga ttcaatgatg
     4141 aaattcggaa aaaatcagaa tacagaaaaa cagacaccaa aggaaaagaa acctcgtgtc
     4201 tataaggtca atcctcgtaa aaaggttgtg attgtcctat gggtactttt aggacttagt
     4261 ttcagctttg cgatattcaa gcactttaca gccattgata cccataccat tcatgaaacc
     4321 accatcatag aaaaggaata tgtcgatact catcatgtgg aaaattttgt agagaacttt
     4381 gcgaaagtct actattcatg ggaactaaac gacaagtcca ttgataatcg catagaaaac
     4441 ctgaaagcat atctgacaga ggaacttcaa gctctgaatg tcgatacagt tcgtaaagat
     4501 attcctgtat cgtcttctgt aaaaggattt cagatatgga cggtagaagc agatggcgac
     4561 aatcaatttg atgtaaccta cagtgtagac caactcatta cagaggggga aaatacaaag
     4621 accgtccact ctgcttatat agtgagtgtc tatgtagaca gtactggaaa tatggtcctc
     4681 attaagaatc cgaccattac tagcatacca aagaaatcag actataaacc aaaagctatt
     4741 gaaagtgagg gtacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
     4801 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
     4861 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tatttacaat
     4921 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
     4981 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
     5041 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
     5101 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
     5161 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
     5221 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgcacc cagtttaaga
     5281 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
     5341 ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt
     5401 gggcttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     5461 tgatgcggga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     5521 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
     5581 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
     5641 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
     5701 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
     5761 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
     5821 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
     5881 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
     5941 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
     6001 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgctc
     6061 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
     6121 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
     6181 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatgg
     6241 cggaatcctg catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     6301 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     6361 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     6421 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     6481 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     6541 cccgcttcta caatattatg tggattctac gacacatgaa attatacttt ctttcttagg
     6601 gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
     6661 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
     6721 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
     6781 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     6841 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     6901 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     6961 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     7021 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     7081 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     7141 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     7201 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     7261 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     7321 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     7381 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa
     7441 ttatgaagtg taatgaatgt aacagggtta aatacaacca aattttaatt tggctttgcg
     7501 ttttatcttt ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta cctgatattg
     7561 caaatgattt taataaacca cctgcgagta caaactgggt gaacacagcc tttatgttaa
     7621 ccttttccat tggaacagct gtatatggaa agctatctga tcaattaggc atcaaaaggt
     7681 tactcctatt tggaattata ataaattgtt tcgggtcggt aattgggttt gttggccatt
     7741 ctttcttttc cttacttatt atggctcgtt ttattcaagg ggctggtgca gctgcatttc
     7801 cagcactcgt aatggttgta gttgcgcgct atattccaaa ggaaaatagg ggtaaagcat
     7861 ttggtcttat tggatcgata gtagccatgg gagaaggagt cggtccagcg attggtggaa
     7921 tgatagccca ttatattcat tggtcctatc ttctactcat tcctatgata acaattatca
     7981 ctgttccgtt tcttatgaaa ttattaaaga aagaagtaag gataaaaggt cattttgata
     8041 tcaaaggaat tatactaatg tctgtaggca ttgtattttt tatgttgttt acaacatcat
     8101 atagcatttc ttttcttatc gttagcgtgc tgtcattcct gatatttgta aaacatatca
     8161 ggaaagtaac agatcctttt gttgatcccg gattagggaa aaatatacct tttatgattg
     8221 gagttctttg tgggggaatt atatttggaa cagtagcagg gtttgtctct atggttcctt
     8281 atatgatgaa agatgttcac cagctaagta ctgccgaaat cggaagtgta attattttcc
     8341 ctggaacaat gagtgtcatt attttcggct acattggtgg gatacttgtt gatagaagag
     8401 gtcctttata cgtgttaaac atcggagtta catttctttc tgttagcttt ttaactgctt
     8461 cctttctttt agaaacaaca tcatggttca tgacaattat aatcgtattt gttttaggtg
     8521 ggctttcgtt caccaaaaca gttatatcaa caattgtttc aagtagcttg aaacagcagg
     8581 aagctggtgc tggaatgagt ttgcttaact ttaccagctt tttatcagag ggaacaggta
     8641 ttgcaattgt aggtggttta ttatccatac ccttacttga tcaaaggttg ttacctatgg
     8701 aagttgatca gtcaacttat ctgtatagta atttgttatt acttttttca ggaatcattg
     8761 tcattagttg gctggttacc ttgaatgtat ataaacattc tcaaagggat ttctaaatcg
     8821 ttaagggatc aactttggga gagagttcaa aattgatcct ttttttataa caggaattca
     8881 aatctttttg ttccattaaa gggcgcgatt gctgaataaa agatacgaga gacctctctt
     8941 gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa gacaataaaa
     9001 attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa aataaaaatt
     9061 ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac tacctgtccc
     9121 ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg gcagagggca
     9181 ggtttttttg tttctttttt ctcgtaaaaa aaagaaaggt cttaaaggtt ttatggtttt
     9241 ggtcggcact gccgacagcc tcgcagagca cacactttat gaatataaag tatagtgtgt
     9301 tatactttac ttggaagtgg ttgccggaaa gagcgaaaat gcctcacatt tgtgccacct
     9361 aaaaaggagc gatttacata tgagttatgc agtttgtaga atgcaaaaag tgaaatcagc
     9421 tggactaaaa ggcatgcaat ttcataatca aagagagcga aaaagtagaa cgaatgatga
     9481 tattgaccat gagcgaacac gtgaaaatta tgatttgaaa aatgataaaa atattgatta
     9541 caacgaacgt gtcaaagaaa ttattgaatc acaaaaaaca ggtacaagaa aaacgaggaa
     9601 agatgctgtt cttgtaaatg agttgctagt aacatctgac cgagattttt ttgagcaact
     9661 ggatccagga gaacaaaaac gattttttga ggaaagttat aaattatttt ccgaacgata
     9721 tggcaagcaa aatattgctt atgcaacagt tcataatgat gagcaaaccc ctcacatgca
     9781 tttaggtgtt gtgcctatgc gtgatggaaa actgcaagga aaaaatgtgt ttaatcgtca
     9841 agaactgtta tggctacaag ataaattccc cgagcatatg aaaaaacagg gttttgagtt
     9901 gaagcgtggt gaacgtggct ctgaccgtaa acatattgag acagctaaat ttaaaaaaca
     9961 aactttggaa aaagagattg attttctaga aaaaaattta gcagttaaaa aagatgaatg
    10021 gactgcttat agcgataaag ttaaatcaga tttagaagta ccagcgaaac gacacatgaa
    10081 aagtgttgaa gtgccaacgg gtgaaaagtc catgtttggt ttgggaaaag aaataatgaa
    10141 aacagaaaag aaaccaacca aaaatgttgt tatatcggag cgtgattata aaaacttagt
    10201 gactgctgcg agagataacg ataggttaaa acagcatgtt agaaatctca tgagtactga
    10261 tatggcgaga gaatataaaa aattaagtaa agaacatggg caagttaaag aaaaatatag
    10321 tggtcttgta gagcgattta atgaaaatgt aaatgattat aatgagttgc ttgaagaaaa
    10381 caagtcttta aagtctaaaa taagcgattt aaagcgtgat gtgagtttaa tctatgaaag
    10441 cactaaggaa ttccttaagg aacgtacaga cggcttaaaa gcctttaaaa acgtttttaa
    10501 ggggtttgta gacaaggtaa aggataaaac agcacaattc caagaaaaac acgatttaga
    10561 acctaaaaag aacgaatttg aactaactca taaccgagag gtaaaaaaag aacgaagtcg
    10621 agatcaggga atgagtttat aaaataaaaa aagcacctga aaaggtgtct ttttttgatg
    10681 gttttgaact tgttctttct tatcttgata tatatagaaa taacgtcatt tttattttag
    10741 ttgctgaaag gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg aaaaccctta
    10801 aaattggttg cacagaaaaa ccccatctgt taaagttata agtgaccaaa caaataacta
    10861 aatagatggg ggtttctttt aatattatgt gtcctaatag tagcatttat tcagatgaaa
    10921 aatcaagggt tttagtggac aagacaaaaa gtggaaaagt gagaccatgg agagaaaaga
    10981 aaatcgctaa tgttgattac tttgaacttc tgcatattct tgaatttaaa aaggctgaaa
    11041 gagtaaaaga ttgtgctgaa atattagagt ataaacaaaa tcgtgaaaca ggcgaaagaa
    11101 agttgtatcg agtgtggttt tgtaaatcca ggctttgtcc aatgtgcaac tggaggagag
    11161 caatgaaaca tggcattcag tcacaaaagg ttgttgctga agttattaaa caaaagccaa
    11221 cagttcgttg gttgtttctc acattaacag ttaaaaatgt ttatgatggc gaagaattaa
    11281 ataagagttt gtcagatatg gctcaaggat ttcgccgaat gatgcaatat aaaaaaatta
    11341 ataaaa
//
LOCUS       DACEUF010000081        11253 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.085, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000081 DACEUF010000000
VERSION     DACEUF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.085"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(40..1419)
                     /locus_tag="IXG20_002157"
     CDS             complement(40..1419)
                     /locus_tag="IXG20_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HAQ1610338.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(1440..2987)
                     /locus_tag="IXG20_002158"
     CDS             complement(1440..2987)
                     /locus_tag="IXG20_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1610339.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3236..4015)
                     /locus_tag="IXG20_002159"
     CDS             complement(3236..4015)
                     /locus_tag="IXG20_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1610340.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4090..4548)
                     /locus_tag="IXG20_002160"
     CDS             complement(4090..4548)
                     /locus_tag="IXG20_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1610341.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(4560..5819)
                     /locus_tag="IXG20_002161"
     CDS             complement(4560..5819)
                     /locus_tag="IXG20_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HAQ1610342.1"
                     /translation="MKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREARNYLIN
                     QMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVVTALET
                     MRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVKNRHGQ
                     TKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIPGIGRF
                     KARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIKPNSNQ
                     FVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARINYSNPT
                     NPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKGGITHH
                     PDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(5935..6249)
                     /locus_tag="IXG20_002162"
     CDS             complement(5935..6249)
                     /locus_tag="IXG20_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1610343.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(6395..7891)
                     /locus_tag="IXG20_002163"
     CDS             complement(6395..7891)
                     /locus_tag="IXG20_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ1610344.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(8069..8548)
                     /locus_tag="IXG20_002164"
     CDS             complement(8069..8548)
                     /locus_tag="IXG20_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HAQ1610345.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            complement(8924..9169)
                     /locus_tag="IXG20_002165"
     CDS             complement(8924..9169)
                     /locus_tag="IXG20_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1610346.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            9274..10047
                     /locus_tag="IXG20_002166"
     CDS             9274..10047
                     /locus_tag="IXG20_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610347.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEADMVIKIF"
     gene            complement(10276..10524)
                     /locus_tag="IXG20_002167"
     CDS             complement(10276..10524)
                     /locus_tag="IXG20_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1610348.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(10623..10937)
                     /locus_tag="IXG20_002168"
     CDS             complement(10623..10937)
                     /locus_tag="IXG20_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610349.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFRLFRDQIIGISQT
                     TK"
BASE COUNT         3500 a         2317 c         1684 g         3752 t
ORIGIN      
        1 tcatttcttc cgttttaaag aagaaatgat tttcatagat caacctttaa ctaaagctag
       61 attcttttct aaaaagaatg gtgtgtagta gcccacaccg ctggacaacg gtaaaacttc
      121 atccggcaac tcgatgccca gttcgttacg gatgtactct tggcgagaaa tgattcgcga
      181 ccaaacattt ggatattctt tggctagttg atcacgaagc gactcatcag ctagcgcaat
      241 cccactctca cagcttgcgc cggcatagcc agcaactgat ggaataatat caatttgtag
      301 taacattccg gacttaagag taatttctga gtcttttttc attggcgaag acatccactc
      361 ttcatcagcc gtaagatgcc ccggatttaa gtgccatttg tattccgatt taggcaagac
      421 tttgcctatt actgaatata gctctttacc ttgcattcct attttgatat tttccaacca
      481 tgcgaccacg gcgtcgtagt atggagctgc aactttttca atgtaatcct tttgcccttc
      541 tggtaggtca tctgtcgttg caactgcata gccacttcga cttgctaatc cgcctttaaa
      601 gcccgtagta attgacattt tatcgcctag tttaacttgt ttatcagttg gatatatata
      661 ggcgttctca aaccgttccc cagttgcagc aattggaatg acattatttg gttggccgta
      721 cttaactaag tggttaccaa tttccatttc tgtttttcct acctctactt catcaatagc
      781 gttcatcaca caatctgaag ccaaggatga gccaaattca taatatgcca cttcattagc
      841 agtatttaat gagcgtactc cgtaatcact gtgaatgaac aggtcggtgc gattgctaac
      901 ggcatcttcc cctactaatt ctacaatcga acttacaatg aatgccggca aatcttggat
      961 ttggtagtta tcctcttctg aagtaaacat cttccaacca acaagcccaa ctttcttacc
     1021 tttactgatt ttagcattgg tcaatacttc agttagtggt ttctcgttat ccatcggttg
     1081 atccggtaat gagaagagtg gcgtgtggat taattctgcc tcaattcttg aatagtccac
     1141 cattttagta ttttcatttc ctagaactaa gaaagcttca cccgtctgat gtaataccaa
     1201 caagctttct tcaaagcgag taacaaaacc tgttaaataa gagaagttcc cgccatgttc
     1261 caaatcacaa tagattacga gggtatctat ctctgacttt tccatcgctg caagtacctt
     1321 ttctttacgg aacttcattg tttcatcagt caattgatat attttagctt cattatcttt
     1381 tttaggtggg ttaacttttt ttagagttac cttagtcata ttgtcactac tttctgtttt
     1441 tatttacgct tcatttcatt tttcagagat cgagttgcgt tttccacttg tcctccatat
     1501 accacttgta aattattttc gttaatttta acgattccca ttgagccggt ctttttcaag
     1561 atatgttcat caattgttgc tgtatcttta agaactagtc tcagccgaga gatacagtta
     1621 tcgatttctt gaatattacc agcaccacca agtccttgca agattagttt gccaagttcg
     1681 ttttctgtca tttcttcgtc ctgatcttcc aacacatcat cgttggatcg accaggagtt
     1741 ttcacatcaa atcgctcaat gacatactta aatacaaagt aatatgcaac tgcataaaca
     1801 aggcccaatg ctacaactaa gtaccaacga gtataagtac cttgtaaaat tccgaagata
     1861 gccaaatcaa taaatccacc accggcattc ccaactgcga ctccgaatag attcattaac
     1921 ataaaggata aaccagccat gaatgcatgg aagacccata gaatcggtga gataaagata
     1981 aaggcgaatt caattggctc agtaatcccg gttacaaaac aagtagccgc tgctgcaatc
     2041 aacatccctt taatctttgg acgttgcgcc aaatatgaag ttttgtacat tgcatagcat
     2101 gctgccggca atccgaagac cataaatgga tgagtacctt gtgtcaagaa gcgagtagca
     2161 cttctcaaaa tatccatgtc ttttactggc aatgctaatt cagtattgaa gatggttaat
     2221 gctccagaaa ctgtttcacc attgatagtt gccgtaccgc cgattggtgt aaatcgaatc
     2281 aactggttta gtacatggtg aagacctgtt ggaatcagca ggcgctctga gaagccatat
     2341 aaaaaagttc caattacgcc tgacttacca atcaaaccgc ctaaagcatt gatagctgta
     2401 ttgacaaaag gccagatgaa aaatgaagcg attgagatga ttggaatagt taggatgatc
     2461 ataattggaa caaaacgatt gccactaaag aaactgaaag ctgctggtaa ttgttttgtg
     2521 tgatagcgat tgtggatcat ggctgtccat aatcctgcaa tgataccgcc gaaaacaccc
     2581 atacgatatg tgaatgtgcc aagtaaagtt tcatacatat tggaagtttc tactgctttg
     2641 atttgactca ttccgtcaga catcaatgtt tcaacagaag tgctttccgc tgtgatgcct
     2701 ctaagctgaa gcagataatt gataactaca tggaacagaa taaatgcaat aaccactgaa
     2761 aatgctgcaa tttccttatc ttctctagac atcccgattg ctactgaaat agcaaataga
     2821 ataggaagta gtccaaacat cgtaccaaca atagcctgta ctccaccaac aaatccttgg
     2881 atcacggttg cattagcgaa gctttctccc accaaagtag gattttgtaa aattgaaacg
     2941 attcctagta gtagtcccga tgcgacaata atagacaacg gacccattaa tgatttccct
     3001 aacttctgga tataagttct caatataaac accttcttct ttttagttga tataaacgtt
     3061 tacatccctt gtacaacgaa agtatagcac cctgaaaaag cgctaacaat acgataggtc
     3121 tgataaaaat caacattcag cacttggttg ttcattcaaa acgtgaatct tcaatcacag
     3181 aaaaatacgc gctatcaagc acgtatttta ggtgtaaata tatgcatcaa ctttattaat
     3241 caacgctact ttgtttgtaa tagttaatat acccttcata cagtaagcta agagcaagat
     3301 tcaaggtgca gaatgatgag ttgttcaact cattcacatc attaatgttg gtcacttgat
     3361 agtataggct atagtctgac agactggcta agttattttg accaaatcgt gtaactgaga
     3421 ccaactgtgt ccctttcata tggattcttc ttaaagtatc atcaatccga tcaacacgtc
     3481 cggacaatga tatgacaaat agtaaatcat tcgaggtcaa agcttttgaa atcaggtcta
     3541 attcattctt ttccggtaca acgataatgt agattcccaa gttccacatg cagcgtgcaa
     3601 actcattgag catcattctt tgtccatatc ctgttgcgta ggcaaatatc ttatctgcct
     3661 ttctaatcgt ttcatatata tcctcaacac ttttatcatc ttcaaacaat ttggttgtcg
     3721 cttgaacatc tccgagtaat acttccggta aaaacaaact acttttatcg actctggttt
     3781 tttgcatgcg tgcaaattcc tctttaaggt aatatcgaaa ttcgccatat cctttgaatc
     3841 cgagtttctt agttagtcta ataatcgttg agtcagacac atttgcttgt tttgctaatt
     3901 ccgatacagt ttgactgatg ctgatttcca tattttgagt gataaaactt aatacataat
     3961 catcattctc attcaattta ctgtgatgtt catttactaa ttgctccaat gtcataatca
     4021 gacctccaaa aataataaaa aagattgctc tctcatttta atacgaaagc acaatctttt
     4081 taagtactat taaattttga aaagaagttc tccttcagaa accttttttt cagagacttc
     4141 tagtttaata cccttatctt cgggaaatag aaccataaca acatctgaca gtttttccgc
     4201 cttgatttgg tcacaatcaa aaatcaccaa tgactcgccg gatgatactc gttgaccttc
     4261 ttcaacgagc acttgaaacc cgactccctt taactgaaca gtatcaattc caatatgaat
     4321 caataaatct ctaccttgat tactagtcaa tgtgagtgca tgctttgttg gaaatatcga
     4381 tttaatgaca ccgtcaatcg gtgctaccac cttagcgttt gttggttcaa cagcgaatcc
     4441 ttcgcccatc atcccttgcg aaaatacagg atcgcttact tggctaaggg gaatcagatt
     4501 tcctgatact ggagcaacta atttttcttt tcgactaaaa aatcccattg acttactcct
     4561 taatcttttg aaatgtccat cacaaggttt aaaagaacat ccgccccttt tttgacatcc
     4621 tcaaaatcag tccactcatc aggatgatgt gtaatgccac ctttggatgg aatataaatc
     4681 atatttgttg ggacaaagtc ggtcatcatc atcgcatcat gtccagtacc attgttaatg
     4741 acttgatagc tatagcccaa ctgatcgcaa atatgtttca tcttctcaac attaccttga
     4801 tccataacac ttggatttgt aggattactg tagttaattc gagccatttc ctccagctca
     4861 gttctaactc cagtttcccc ttgaatcact gataactcat tcaacaactt ttctttaatc
     4921 tcttgaggat tagttgtctt tgtatcaaag gttctgattt cgattaaagc ttcgacaaag
     4981 tcagggacaa attgatttga gttcggtttg attgtaagtt taccgacggt tccggcaatc
     5041 ccttcaccaa gattcttcat ccactgttca acaaaaagaa caaatttaga agcagcaact
     5101 aaagcatctt tccgtttatt cattggagca gtcgagtcct cagtttctcc ataaaaacgt
     5161 gctttaaaac ggccgatgcc aggaataaat tcaatcaatc cgatatcttt gccttcatct
     5221 tccaggactg gtccttgttc gatatgaagt tcaataaaat ttttgattgt ttttggatct
     5281 cgcttagcgg cctctaaatc tggttccact ccataagcac gaagagcttc caattttgtt
     5341 tgtccatggc gattttttac tgtttccaac tcactcatgg ttaatgttcc catcatagct
     5401 cgtgaatttg agacacccgt acttggccca aatgtctcgc cctcctctgc attcatcaga
     5461 attaggtcaa ttgggtaatc attttcaaaa ttattttctt ccagaatccg cattgtttcc
     5521 agtgcagtaa caacgccagc cactccgtca aatctgcctc cattaacaac tgaatcataa
     5581 tgagagccaa tcatcactga tggtgcatca gcctttttac cggctctacg accaaataat
     5641 gtagagaagc cgtcttccca aatctctaaa cccagttgtt ccatttgatt gatcaaataa
     5701 tttcttgctt cacgatcttc tttagagtaa gcagagcggg tcactccttc acctggagtt
     5761 gcattgtact cggctaaatt ttcgatatcc ttttttatac gctcaaaatt tgtcttcata
     5821 attaaatcct ccatttccta caagtgaatg ttagcaccca tagaaagcgg ctacaatata
     5881 atcaatgaag attgaatcga tattcgaaaa ctgaatattg cttcggttaa tgacttagaa
     5941 atcattatcg tagattccac cataagaact ttttctcgtc tcataacaca actcgagagt
     6001 ggattggttt aaaaaactct cgataaatgc gtccacttta ttgacacaga tccatggatc
     6061 gatcgcacct tgaaatttaa acccagaaat agtcccatca tcttctatac cgattaccag
     6121 cttacctcca tcggcatttg caaacgctac tatgtgtttt ataatatctc taggcttaat
     6181 ccgtgcactt tttctatcaa aatactgatc ttctttttca cttataaact tttgaatctc
     6241 ataatccatt taaaatttcc cttcttaaat ttttttatta tagagccgta tattcaattt
     6301 ccacaaaaaa ataatctaaa acaaaacaga caccttcatt ttgatatgtt ctcccacata
     6361 tgaatcggtc gctatacagc attacatata gtaattattc atactgcgaa ataaatagct
     6421 ccaaatcttt agctggcgta gttaagctta aattcgaaat gaaagtttca ttgactattc
     6481 cactttcatc aaatcccttc ttcaaccaat cagagttcca caacgtaaat cctttaggat
     6541 tagaaacttt cttacataat tttgcactat aggatttctc tcttgaatga attaaagaaa
     6601 gtctttgtaa ccaaatttca actatatccg tattaggagt attaacaaat tttcttaaga
     6661 ttttatccaa gtataagttg atttcttcta cagcaataaa ctcaaaaagc ttacctaaaa
     6721 tcacaataca agcctccaaa gttctaggat tcttttgcat tatattaaca agaatactaa
     6781 tcaattggta gatatcatct ggtcttttgt ttaaagtaga aactctatgt gtatagactt
     6841 cacttagcgc tttacttaaa ctcccgcaat ttggatattt gtctcctaaa agctttacct
     6901 gtagcaaatg cttttgaagg ctaagcttaa acttccactt attattactt ttctctgtaa
     6961 aacttgcatg tttaagatcc cagtaaattt tatcaggctt aattgcatct aaaattatat
     7021 tatccgataa gaatgtttta tttgaattta gcttcaaatt tagatccgat aatacttcag
     7081 ataacaatct gataatcact tcagcttgat cttttcgaac cgaaaaaatt ctgtaatcat
     7141 ctctgtaacg aagtattttg aactctttaa tctctgtttt ttctatcttg ttcagtaaca
     7201 ttttatcagc atacccaagt actatttcag ctacaaaatc catcaataca tttccttgag
     7261 gtataccgtt cgtttgtcca ttttgtaaat atcgtatttt agaatctatt aagtttccaa
     7321 cgccttgagt atcttttcta tgtgtcttag cccattcttt tgaatgtact gcccacggga
     7381 tagtatgtgt gtacattgag ctataacaat cggtaatatc tgaatgaatg cagtagtcat
     7441 actccaaagc ataactaatc tgtgcttgtt caaggttctc ccaccaattt aaaatagttt
     7501 ccgcagtatc gctctttgaa gaaatagatt ccacaggaag tgatatacat tcaattcgct
     7561 tgtcggcttg gaattcttca aacttatttt tgatgtgatt ccaattattt ttctgtgtaa
     7621 tcccgtttac taagtctaca tatactaatg gatgtaatat ttgaaggggt ctccaagcat
     7681 aattaccatc tttattagct aatattcgat gattaacatc aactgcctca gagagcgatc
     7741 tcttatgtct aactagttca gtaaatggtt tgttttttaa taaatccact gctttctcaa
     7801 tgcctactga taaatcaata tagttgggca acgatgtact aaaaaaactt gatgttttta
     7861 aaaagaattg ctttgcttct tgatgattca tctctaaaat actttttcta gtcctagtca
     7921 aattcaaaat ctcctagatc aattaagttg taatgtaaga ggatacccta aggttacaca
     7981 ccctatttac tattatacca gtattcattc gtgatacata agggtgtaac attaagaact
     8041 ctatctaaaa gactacaagt agtcaccttt aattgtgagt aattttcttt atccaatcct
     8101 tgtgcaacct tgtcgtcacc ccttgtatat ccccgtaccc cggatgtttc ggaatatcaa
     8161 aaatgtgctc aaattcctga ttttcatcag tctcccactc atgattcaac ggttcatgcc
     8221 actctataaa ccttgaccat aaaatgttct ttctataaaa ctcaatagga atttcaaaac
     8281 agtaaaaatt gtatttgcta aaatctgtaa aattatcttt acgagcatac tttaaatggc
     8341 aggtatctcg ccacaaccaa attaaatctc gatccaatgg taactgaatt cgtttcgtat
     8401 actcttcttc aagccaatga agtgcaccaa ctgcattcgg attatcgaaa aagacagata
     8461 attcagtata gcgtttcttt ggataaataa atccttctga atcaacagat tgcaaaaaat
     8521 ctactggttg aaccgtgtac actttcattt attcgccacc tttttttgac tacttatagt
     8581 catttaccaa tttgaaatct gcatactttt tcattcaatt gagcccagca ttaattgtac
     8641 acgagttttc ttcactaacg gatagtatca ggatatacta atactatcct gaatagcgaa
     8701 atgaggtagc caaaatgaga aaagtcgatg aatatatcaa aaagaaatcc caaaaaagta
     8761 aagtatggca aagaacattt gagttagagt ataagaaact ctcagatagc ctcaaaaagg
     8821 acacttataa tccaaatgaa caaagtaaag cagataacca ataaagatta ccctcccata
     8881 tttaacattg cttcacgaat cagtaatcaa tgactacaag tagtcattga agcaaatctt
     8941 cataagatac ctgtagaata tccctaatag ctctcaattg atccagctta atatgttgcc
     9001 ggccaccttc aatcttaacc aatgtttctc ttgtcatagc gattttactg agctggagtt
     9061 ctcgcaccag ttctgtctgt cccattccct tttctaatcg gattcgtctg atattgctac
     9121 cgattacatt ttctttatct attaactttt cttccttcaa aaatagcacg ccttttggac
     9181 taaaattagt cctttttctt attaatctta aagatttggt atgctacaat gggactagta
     9241 ttagtccata ttcaatttta aggaggaatt tttttgaaga aatttgcaat cattctaacc
     9301 tgtgctttac tcacgggagc tataagtggg tgcgccactt ctgacacaaa ttctacaagt
     9361 actgaaacaa gtacaacaac ctcagaaaag aattatccta tacatttttt tccagttcca
     9421 gatggatttg aagcttatta tcatgataaa aaactagatg ttccattgtc agaaagtaac
     9481 tctattgaaa aagttttgcc aattgaaatt gaaaaaaatc aagagaatat aaaactcaag
     9541 gccgagcttc ccatactgaa cagcacagaa attgattggc acgagaatat taaagtaact
     9601 attgaaaaag gagcgttagc acaactttat actgaaaaag ttgaacaatt ttttgagtct
     9661 ggtgccaata aagacttttc aaaaataagc gattattcgg ataacttttt taatgagacc
     9721 caagatgaaa tcacgaactc aacttttatg gctgaagata tgtacaatat gacgttaaaa
     9781 aaaataaaag cttacgacca tccattaaat ctgacactaa ataatggatt actctctttt
     9841 gtattagcag gttacgttga ctatgaatat tcaccagatt atttggagac acctcttcaa
     9901 cacgagaaca atgatattaa ttttagattt gtctacgagt caaaaagtaa aacatggtta
     9961 gttgatcaat taagcactat aaacacaagc ttccatgaat tacctaaacc tgaaaatgaa
    10021 gctgatatgg tcattaaaat cttctaaaaa agaagcgttg aaggagaaat attcctttca
    10081 acgctctttt ttatatgctt agaaacataa ataaattgta tactttcttt ttagccccta
    10141 ttacttttca cgaaaaatat cttttttcga cactagcgtt tctctactta taatattgta
    10201 cacagttttt gtattattga aaaattactc attatttagc attaatccac atcgttttat
    10261 caatcgacct tcatactaaa ctactttaaa gtcatcttcc ttctcatttt tcattaaatc
    10321 agatacataa gcccatgtac ccgttcgttg taaataaagt tcttcttgta gcagctccca
    10381 ctttctctta gaaatcataa tgacgccatc gttcaatcca tttttagaat ccctagtaat
    10441 ttctatttca gaatttgaat gaactacttc ttgcaacact ttacctatat tttttttgaa
    10501 gtttgttgga gtaatagtat ccaaaatgaa cacctctctt ctctatacct ctattatgaa
    10561 gcgcccatca aataaagcaa gcgttgagga ataattcttt caacgcttgc tttattaagc
    10621 tttcattttg tagtttgact aataccaata atctgatctc taaacaatcg aaaaacttct
    10681 tgtggttgat aataatctgt atctatttca aatgtttctg agaacaattc attgaatttt
    10741 tctactgaaa tgatgatttt accattctcc tctcgactgt tttctattat ttctgcttct
    10801 aattcaaata aatctagtga cgaactaaaa tcataaatcc tgtcatattg aacaaaaaaa
    10861 tcaagaaaaa gttgctcaaa ctcttcctta tttatcacta gtgtttcaag cggaacaact
    10921 ttattttcat cagccattct tccaatttcc tcactttcat tttcaaaaaa tcgtaaaatt
    10981 gatatagatt ctttgtctct aatcactcgt gatattatct tgacagtcct acatctaatt
    11041 caattaaagt ataagccact ttcaaataaa agattacaaa gtaaatggca agccaacaaa
    11101 ataaccatct gcttcacgta ttacgatttg ataattctgg atttcgctcg gaagttcata
    11161 ataataatta acagtttctg ctttgccact ttcaatatat tctggattat tcaatatcat
    11221 agcatactca taaagttctt caccattttt att
//
LOCUS       DACEUF010000082        11028 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.086, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000082 DACEUF010000000
VERSION     DACEUF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.086"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..997
                     /locus_tag="IXG20_002169"
     CDS             <1..997
                     /locus_tag="IXG20_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1610350.1"
                     /translation="TNRYHLPFSRKLTNKINIKKIFSPSEPVWKTFEDIEYTDQLSDL
                     WDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAEELFALAKEKGLF
                     IQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENTHEFSTANSYLYG
                     HACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMKISIKSSYFRIKE
                     RPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGVLTYIDEQGSYHE
                     EKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIKSIQESEGKI"
     gene            1086..1391
                     /locus_tag="IXG20_002170"
     CDS             1086..1391
                     /locus_tag="IXG20_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ1610351.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            1570..3585
                     /locus_tag="IXG20_002171"
     CDS             1570..3585
                     /locus_tag="IXG20_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1610352.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            3587..3871
                     /locus_tag="IXG20_002172"
     CDS             3587..3871
                     /locus_tag="IXG20_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1610353.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            3897..5270
                     /locus_tag="IXG20_002173"
     CDS             3897..5270
                     /locus_tag="IXG20_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1610354.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(5353..7368)
                     /locus_tag="IXG20_002174"
     CDS             complement(5353..7368)
                     /locus_tag="IXG20_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ1610355.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(7390..8604)
                     /locus_tag="IXG20_002175"
     CDS             complement(7390..8604)
                     /locus_tag="IXG20_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1610356.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            8825..9769
                     /locus_tag="IXG20_002176"
     CDS             8825..9769
                     /locus_tag="IXG20_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1610357.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            10065..11009
                     /locus_tag="IXG20_002177"
     CDS             10065..11009
                     /locus_tag="IXG20_002177"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1610358.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         3626 a         1975 c         2120 g         3307 t
ORIGIN      
        1 tacaaatcgc tatcatttgc ctttttcaag aaaacttacg aacaaaatca atatcaaaaa
       61 aattttttcc ccatctgaac cagtgtggaa aacattcgaa gacatcgaat atactgatca
      121 gctgtctgat ctttgggatg atcctgagat ccaattagtt gtcatcacta cacctagtca
      181 atttcattat gaatatggaa aaatggcact cgaacatggt aagaatgtac tagtagagaa
      241 accttttgca gaaacgtcaa aggaagcaga agagttattt gcattagcaa aagaaaaagg
      301 tctatttatc caatgttacc aaaacagacg gttcgattct gacttcttga cagtccaaaa
      361 agtaatcgaa agcggaaaat taggggatat tttagaagta gaaatgcatt atgattattt
      421 cagaccggaa gtaccagaaa atacgcatga attttctact gcgaacagct atttatatgg
      481 acatgcttgt catacagtag atcaagttct ttcttatttc ggcgaaccag atgatatcag
      541 ctatgatgta cgtcaacttt tagggaaagg acgaatgaat gattacttcg atctggattt
      601 tttctatgga aatatgaaaa tttcgataaa atccagttat ttccgcatca aagaacgtcc
      661 aagtttcgtc gtttacggaa aaaaagggat gttcgtgaaa caaacaaaag atcgacaaga
      721 agaacatctg aaaatgtttt acatgcctga tcatgaagac tttggtatcg atttgcccga
      781 acattatggt gtattgacct atatagatga acaagggagt tatcatgaag aaaaagtaat
      841 ttccgaggtt ggtgattaca gccgtgtcta cgaagggatt tacgagacat tagttaatgg
      901 cgcagaaaaa gtcatcaaag atgaagaaac aatcttacaa atgaaaattt tggaagaagg
      961 aatcaaaagc attcaagaat cagaaggaaa aatatgacat atttgtaaga atcgggtttg
     1021 ttttttgacg cataaactgt cttattgtac gatgaaaaag tatagaacta ctaggaggaa
     1081 ccaacatgac aaattatgac ggtgaaaaac acaaacgatt aagaggttca atggccccta
     1141 aaacagaaga agaagtggct ttagcaggaa ctaattctgg tgaaacagaa gacaaagaag
     1201 aatcattagc tggcacaaac tctggtgaaa cagaaaataa agaaacagct ttatctggtt
     1261 caaattctgt agaagaggaa acaaagccta atgagcccaa tcactttgaa aaagatatcg
     1321 aaaaagcggt tgaagatgtc aaagaaaaat ttgaagaact gacctcaaaa aataaacatg
     1381 atcatcattg atcggacgaa atcaattcat aaaagcagga gacatttggt cacctgcttt
     1441 ttttctgtat ccattcgata gaaatcgttc ggtacagtcg taaaaaaaat ccaactgtaa
     1501 gttgtaccaa acacccctcg atttaaaagc gctaccatac tagaatataa gtaggaggtg
     1561 aacagcgctg tgaactacaa aattagttat ctcctgaaaa caccaagaga aatcagtcta
     1621 gaaaaagcag cagagatcat cgaagtaaat acaagcgagg cgatcaaaca attaactcct
     1681 tttgccgaat atgtggatga tacccgtatc cgtataaaga aattccgagc aatcgactgg
     1741 gcagaaaaat ttttggatat tactcgattt gaagctgtgt ataccgaaca agagcgaaga
     1801 gatcttcttt atttgatggt ttattcgact gcaacgtctg tttctgtttt tcattttcaa
     1861 gattttcttc aggtttctaa agggacagtc ctgtcagata tcaaaaagat aagaaatgaa
     1921 ttagaagtgg aagattgtca gctgtcgtat actaggaaaa ctggttttag gctgctaggg
     1981 tcagaagcaa tattaagaag agaagcaaaa aatcgcactg ctcgtttgat gcagtcactt
     2041 gctggaagat ttgggctcac gtatttaacc gcaacccttt ccattgatta ttacgccaaa
     2101 gttagagatt tgctagataa aacgatttca ctatcagatc ttcgattcgc accaaatcga
     2161 atggatgaat tagcttatta tttgagtttg acaaccattc gaattggaaa aacatccatg
     2221 gaactggtaa aagatgctga actgttggct tctcttgcag tcactgacat tagtcgacaa
     2281 gtattgactg gccttggcta ttccgaacat caaaattcgg agattatatt tttctctctt
     2341 tgcttagcat cgatcgttga aggagatgtc caggagcctg cacttgattt tttgctggaa
     2401 tgttcttcag aagtcatcca tcgcatggaa ttcctgatgg ccgtagaatt tgcttcgttt
     2461 cgtgaattat tgatgaatgt cttttatcac ttggtccctg cctattttcg tatttcctat
     2521 tctttttatt tacctaatgt gatgatcgat caaattaagc atcaatatgc ttccatctac
     2581 gaaatgacaa gaaaagcatt acttccgtta gaaagacgaa tagggaagag catccctgaa
     2641 gaagagattg gttttttcac tatactattt ggcggagaga tcagaaaagc ggacgcagaa
     2701 gaaagaaata gaaaaataag agccgtgatc gtctgtccaa gtggcattag ctcttcattg
     2761 atcttaaaaa gtgaacttca gcaattattc ccgatgatct tttttacaga gactaattca
     2821 gctgaccgct tacacgaggt tgacgaaact tcttacgata ttattttttc tactgtacct
     2881 ctcacactta ctgataagaa aaagtctctc tatgttctgc atccaatcat gactgacttg
     2941 gaaaaaaatc agctgatcaa tcaagtacaa agagattggc tgataccagg attttcaatg
     3001 ccttctgccc aagaattgtt gtcggcgcta ctgccttata tcgaattaaa agaaggcgta
     3061 gacgaagaaa agctctatca agtattaaat cgtaaaatga acaaactttt agaaaagaag
     3121 gaggatcaac gtcctatgct atctgaatta ttaacagaag aaatgatcca attatcggat
     3181 caaccaaaaa actggcaaga agcaatcaca tttgccgcac agccgctatt atcagaaaat
     3241 aaaatagaag aaagttatat caaagctatg atcgagcgcg tggagcagta tggtgcgttt
     3301 atccatattg gcgatcacat cgcattacct catgctcgtc cagaagatgg cgtaaacgaa
     3361 gtagggatgt ctttgctgaa acttcaacaa cctgtggatc tgcttgataa tccagcccat
     3421 cccattagtt tgttcatctg tttagctgct atcgataatg aggcacatct tcgggctttg
     3481 gcaaatttaa caaagctact ttctaataaa caaaatcttc aagatttatt acacgcatca
     3541 acaaaagaag aaatattagc gatcataaga aaaggagacg aatgatatga aaatttttgc
     3601 agtatgtcaa agtggattag gaacaagttt tatggtagag atgaacatca aacaagcact
     3661 tgcagatctt ggagcagatg tggacgagtt tgaagtaagt catacggatg ttggaagtgt
     3721 atctagcgat atggctgatt atttcttcat tgagcattct ttgaaagata cgctaacttc
     3781 tatacctgat gaaaagctcg tacctttgca atcgatcatt gacagcgaag aagtaaaaga
     3841 aaaagtaacg aagatcttgt caaaagaata aaaatgattc aagggaggaa ctatcaatgg
     3901 ataaattgtt gaatttgttt gtgtcaatcg tcagtcagcc ctccatcctc gtttctttga
     3961 ttgcactgct tggtctcgtt cttcagaaga agaaattttc agaagtgatc caaggaacta
     4021 tcaaaacatt tgtcggattc ctagtactga tcggcggtgc tggtttaatc agtaattcac
     4081 taaccccttt tgcttcgatg ttccaaacag ctctgcatac tcaaggagta gttcctagca
     4141 atgaagcagt agtggcgatt gctttgcaac aatacggtac acctactgct ttgatcatgc
     4201 tagtagggat gatcgtgaat attttacttg ctagattcac aagatataaa ttcatttttc
     4261 ttacaggtca agcaatgctt tatgtttctt gtctaacagc agtcatttta gtaagcgcgg
     4321 ggtttactgc aaatttgtgg atgatcttat taggcggggt cttcgaagga acactattaa
     4381 ctattacgcc tgctctttgt caaccgttta tgaagaacat cactggaaac aatcaagttg
     4441 cgttagggca taccggaaat atcggttatg cttctagtgg atggatcgga aagttttttg
     4501 gaaacaaaga ccgttctaca gaggacataa agattcctaa aagtttcagc tttttgcgag
     4561 attctactgt atcgatcatg ctcttgatgt cgatcgtata tgtcgcactt gctttgttag
     4621 ctggtcctgg atttgttgaa agagaactaa gcaatggaga caacgctgta atctttgcac
     4681 tgatccaagc tggaacattc acagctgggt ttgttatcgt ccttcaaggg gttcgaatgg
     4741 tgttggcaga aatcgttcca gcattccaag ggattgctaa aaaactcgta cccgattcta
     4801 aaccagcatt agatgttcct atcgtttttc catatgcacc taatgctgta ttaattggct
     4861 tttttgttag cttctttgta ggaacactat ccatgtttgc tatgataggc ttgcaaacta
     4921 cagttatcat tcctggggta gtaggacatt tcttctgcgg agctgcttct ggtgtcttcg
     4981 gcaactcgac tggcggtaga agaggggcgg tattaggttc tgctttcaat agcttattga
     5041 ttagttggct gccattattt atccttccag tattaggaga tcttaaaatc gcttcttcta
     5101 catttgccga tactgattat ctagtaccag gacttgtact tgggaaacta ggagaatacg
     5161 gtcaaatagc cttgacagtt ggaatcatcg ctttcttttt acttgtcgta ttatctagct
     5221 ttctttttgc cggaaaaaga acaaacattg tttctaaaga acagaattaa gcaaacttca
     5281 aaataaaaat tgcttggaca atatcagaag aagatttaaa gtcttctgat atgtcgcaag
     5341 cagttttttg ttttatgcat ttatttttga atagcttgtt aagacaagtg gctgttttgc
     5401 tagaagagga taacttttac cattcatttt cactgttccg cctgttctat cagaagtaat
     5461 cacgatctta tcttttgttt tttcaagcga aatctttcca tctttgatag gtagcgtaca
     5521 attcagtgtt tcaaatgaat ctagattagg ttgtatcata aattcttcat aacctggctt
     5581 tgttggatac aaaccgataa aatagcgtcc taaaagatag attgggcttg ctccccatgc
     5641 atggcataag ctttttccat aaggatctcc atacatactg tacttttcat ttcctgtttg
     5701 agtagggtcg tattcttccc agaaagttac cgcatcatga gataacatcc ctccccaata
     5761 atcaagaatc gtttggtaga cgatattcgt ctgtccgagt ttgcataaag catcttgttc
     5821 atagaatttg aaatatggag tagtaatctg tgtgatagtg tcattcaata agacattctt
     5881 taagatcgat tgctgttggt ctttgttagc aaaatcgaat aaaacagcga aaatattcgc
     5941 atgacgcgtc acatttcttc tcccagattc atagcaatcg ataaaagcgc caagttcttc
     6001 atcccaaaag tagtcaaata ggttttgttt caatagttga tatttttctt gatagagatg
     6061 agtatgataa ttttcgttct ctccatatgt ttcagctaag atatctccgc aagtgatcat
     6121 ggaatgatag gctttcagta ataagacttg ttctgcagca agcgttcctt ctttatccat
     6181 ttcagaccaa tcaataaata tccaatcttt tgctctccca taaataaacc ctaattcatt
     6241 cgtttgctcc atcaagtatc tcattaatga ttgcatctta gggtagacga tgcgtaaaaa
     6301 ttcatagtct ttcgtcatca tatagtggtt ttcgatacca atcaaccaca agatcgaata
     6361 gtcaacgata gtattcatat gctgcttgat ttcattttgt cctcttaatg ctaatatcgt
     6421 tcgtttattg atttcctcgt caaaaaaaag atattgattg atcatgaatg attgatacgc
     6481 atctccagac caaatccagc gatctcgttt gataccgtcg ataaaaaaca aatcactaca
     6541 taacttgaag gcttcttctg atactgacca gatctgattc agtaaagaag aatcgcttga
     6601 aaaagcagct tgtttcgaaa gagataaaaa ttcatgaaaa acttgaagtt gaaccattcc
     6661 agtagaaacc tctggtataa acaaatagcg aaaagcgcgt ttacgagctt tcatttttct
     6721 tgtcattcct atttgcttat agtagcaata atcgatatcc atagcttcta catcggactc
     6781 tccataacat agagtgatag attcgatatg gtcatccaga gatattagtt ctacacagcc
     6841 attcactgct cgcccaaaat cgaataagat tcctccgcca atctccgttt cagtgactgg
     6901 atggacgcat tctttttgat aatagatttg atttggatct tttgcgatat cgacataaag
     6961 cggactccag ccagctggat attcttcaat aaaattactt gctgtccagc caatatctga
     7021 ttttacttcg tctccctcga taaacatcgc tggtagccct gatgtatgtc ctacaaagat
     7081 acgaattttt gctttaccag ctggacaagt cagccatttt ccacatggat gtttttgtcc
     7141 attaatttct acatacccaa ctccttggat cgtcacttta aacatggaag gttgatcaag
     7201 aaagtaatat ctcttaaact tgacgttccg atggcaatca tccatgtacc agtacgctgg
     7261 ccagtcaaaa cctcgttctt cccgttggaa attttgtagc agtccatggc ggatctcaaa
     7321 gtctccagga taccaaagcc atctatcttt atgtttttca tttgtcattt tacacgacct
     7381 ccatcaaatt tattttgttg tcaattgttc ttttatttct tcatctactc ttatcactgt
     7441 ctttctattc gtcctgtgta ctactagaag gctaataagc gcgctaatca acagcaagat
     7501 actgccattg acgaaaacag caagtgtttt actcccaaga gaagcaaaag agagaaagag
     7561 caaaccatat ggtgcaatga aagtacctaa attagcgcca accaataaga ttgaagtatt
     7621 gaaactctcc gcgtacttgg gcgcttggat acttacttgg ttgaataagt aaggaatgaa
     7681 taacgaaaaa gaaattcctg ttaggatcgc accaaaccca atcacataca ggtttatcac
     7741 agtggcaatc aatacaaagc cgattcccat cagtagtaaa gaaagtgtaa gcatatagcg
     7801 atgtgctttt gtctgaagcc agccaaaaca aactccggca gccatacttg ccaattgcat
     7861 gattgttaag atattcgctg cattagtttc gtttcctaga gaataagtcg tcaggactgt
     7921 ggctaattta atcgatacag cattatatac cacgatgacc agcaagagaa aaataccata
     7981 actaatcatt ttaggattcg ttttgattac ttgtcttgtt ggggctgtgc catgcgattg
     8041 aagttcagtt gtttcaggtg taggaacaaa caaaacaaac aagacaagaa taggtaagct
     8101 gatggcgtag actaaaaagg tatattgcca accgaactgt actagctgtc ctgcaacaaa
     8161 tgtcattaaa gctgcaccaa atccttgaaa tgcactttga aaaccaatca tagaggctcg
     8221 ttcatctccg tgataaaagg cagcaatcat gctgactgcc aaagaattga tcaatccaaa
     8281 gccaatacct agtccagctc ttgaagctaa gacaatactg taactttgtg agaagagggg
     8341 gaggatacca caaatcgatg caatcactaa tccgatgatc acagtttgtt tttgtccgat
     8401 tttcctagca ataaaactac taatcaaaac aaaaatagtg accataaaag agggagcagt
     8461 cgttaataat tcaacagcag ataaagaacg cgtggcataa gtagctgaca tactgggaat
     8521 tgcagacgtg actgcaccag cagaagtcaa aataagagag actgacagca gagaaaattt
     8581 catcatcttt ttattcataa gcatgttcct ttcatttttg taagcgctta tcaaaaatat
     8641 agtatgaagt ggttattctc tcaatagcgg aatcacagga agttcatgtc ccaaccatag
     8701 aaagtcttta tgaaaccaaa aatagctttg catataatag cagtacagcg tgaactaata
     8761 agcgaaagaa accgcttaca aaaaggaggg agtcaattgg aactagctga attagacatt
     8821 ttttttgcgt aacgaagaat caatcgaaac tctgcagcgt gaacaaggaa tgaatatcaa
     8881 tgatttaaac ttagcttacc atcttcagac accgccaaaa atccaccgag gggatctatt
     8941 tcaacaggga aatattcata tcagtaaaca ccgtcgatat gcggaagttc ccgagcatac
     9001 acatgatttt gttgaaatca attatatgta tagcggtaca tgtttacaaa ttattaatgg
     9061 agaaaggata gagatggaag aaagtgacgt ggtgatcatt gataaagaag ctactcaaag
     9121 aatcgaatac acagggcaag aggatatttt aatcaacatc ttattaaaag aagaaacctt
     9181 atctacagag atattgaatc atttggcaag cacaaataat ctactttctt cttttttgat
     9241 aaatgcatca aaagaaaaag gggaacatac acaattcatt cattttcgcg gcggaaaaac
     9301 aagtcgtttg acgcatttga ttgaaaatat gctgttgatt tattttgata catacagtca
     9361 taagatccgt tcgttaaatc tctatacatc actattactg atcgaattga ctcatttatt
     9421 agaacaacaa agcttttcat cagcagatgg atgcgcagaa aatgagatgc ttagagtatt
     9481 agattatatc gataagcatt ttacttcttg tacgttaaaa gaaacagcag ctcattttgg
     9541 ctacaatccg gtctatctta gtaataaatt gaaaatgtat tttgatgctt cctttcaaga
     9601 actggttcta aagaaaaaat tcgactttgc gttggaactg attcaagaaa cgagtttttc
     9661 gttggaagaa atcgctaatc gtatcggata taaacaaacg acttctttat tcaaattatt
     9721 caagaagtat tcaacatgta ctcccaatga atggagaaat aaacattaag aagaaaatct
     9781 ttcactaaag catatgaagg aaaattgttt taggtgcgat tgctgtctta ttatatcgtt
     9841 gcagcgaatt tttttggatt tattttcagt tctaaacttt tggttgtatt atcaggggat
     9901 ataggtgtaa acatttgtca tttatctgtt atgcgtattc aggaattcgt ttccatcaaa
     9961 aattataata acaactatta taattaacat gttgataaaa tgaataaaca agactatact
    10021 tttcatgaaa gtaacaaata gttatgaaaa ggagctgttt attcatgaaa aaaacaagtc
    10081 gtaaagtagt aatcgtcggt acaggttttg ttggaacaag tatcgcttat gcaatgatca
    10141 accaaggaat ctctaatgaa ttagtattga tcgatgtgaa tcaagaaaaa gcagaagggg
    10201 aagcattgga ccttttagat ggtatggctt ggggcgatga aaatgtggct gtatggtcag
    10261 gtggctatga agaatgtaaa gatgctgata tcgttgttat cacagctggt atcaaccaaa
    10321 aacctggtca atctcgttta gatttagtta aaacaaatgc gtcaatcatg cgccaaatcg
    10381 taaaagaaat catgggatca ggattcgacg gtattatcgt tgttgcttca aacccagtag
    10441 atattttgac atatatcgct tggaacgaat ctggtctgcc aacatcacgt gttatcggta
    10501 ctggaacaac attggataca actcgtttcc gtaaagaaat cgcattgaaa ttaaaagttg
    10561 acccacgcag cgtccacggc tatatcttag gtgaacatgg tgattcagaa gttgctgcat
    10621 ggtcacatac aactgtcggt ggtaaaccag tgtttgaaat tgtagaaaaa gaccatcgta
    10681 ttgcccaaga tgaattagat gtgattgctg ataaagtccg taatgctgct tatgagatca
    10741 ttgatcgtaa aaaagcaact tactatggta ttggtatgag tactgcacgt atcgtaaaag
    10801 ctatcttgaa taacgaacaa gctgtattgc ctgtatctgc ttatttaact ggtgaatacg
    10861 acgaaaaaga tatctttaca ggtgtgccat caatcgttga cgaaaatggt gttcgtgaag
    10921 ttgttgaatt atcaatcaac gaagaagaaa aagcaatgtt cagcaaatca acaagtgctt
    10981 taagagaagt attgaacact gtattataag aaacaatgaa tatgaact
//
LOCUS       DACEUF010000083        10782 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.087, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000083 DACEUF010000000
VERSION     DACEUF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.087"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(85..801)
                     /locus_tag="IXG20_002178"
     CDS             complement(85..801)
                     /locus_tag="IXG20_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ1610359.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(851..1060)
                     /locus_tag="IXG20_002179"
     CDS             complement(851..1060)
                     /locus_tag="IXG20_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610360.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            1337..2610
                     /locus_tag="IXG20_002180"
                     /pseudo
     CDS             1337..2610
                     /locus_tag="IXG20_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2717..3748)
                     /locus_tag="IXG20_002181"
     CDS             complement(2717..3748)
                     /locus_tag="IXG20_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1610361.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3755..4585)
                     /locus_tag="IXG20_002182"
     CDS             complement(3755..4585)
                     /locus_tag="IXG20_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1610362.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAKTGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4582..5388)
                     /locus_tag="IXG20_002183"
     CDS             complement(4582..5388)
                     /locus_tag="IXG20_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1610363.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5394..6218)
                     /locus_tag="IXG20_002184"
     CDS             complement(5394..6218)
                     /locus_tag="IXG20_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ1610364.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6208..7308)
                     /locus_tag="IXG20_002185"
     CDS             complement(6208..7308)
                     /locus_tag="IXG20_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1610365.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7486..8271
                     /locus_tag="IXG20_002186"
     CDS             7486..8271
                     /locus_tag="IXG20_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ1610366.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8304..8687
                     /locus_tag="IXG20_002187"
     CDS             8304..8687
                     /locus_tag="IXG20_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1610367.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            8763..8894
                     /locus_tag="IXG20_002188"
     CDS             8763..8894
                     /locus_tag="IXG20_002188"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1610368.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            8863..9099
                     /locus_tag="IXG20_002189"
     CDS             8863..9099
                     /locus_tag="IXG20_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1610369.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9177..10148)
                     /locus_tag="IXG20_002190"
     CDS             complement(9177..10148)
                     /locus_tag="IXG20_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ1610370.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3196 a         2511 c         2057 g         3018 t
ORIGIN      
        1 cgtatccaga tcatgaatcg ttttcgagcc tcgcactctg aaaatcagaa gaagtaccgt
       61 cggctcaagc gatattggaa actcttactc aaagattcca cgacattgga gccgctcaaa
      121 cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaatcgt cgatgaaccg
      181 ttgtgcacaa tggtgtcctg aacgtacagc gacaccttcc atatccagag cagtcgccac
      241 atcacgggga tggatgccat ccaaattaaa agcaaccacg ccagtatgat gttcaggatc
      301 ttgcggtccg taaatggtga taccgccgat cgcttgtaat ttaggtaaaa cgtaggcaac
      361 cagttcttgc tcataacgat gaatattctc cataccaatc tcattcaagt aatcgattgc
      421 tcctgcaatg ttcggagtac cagcttcaaa tttccatggc agctctttcc atgaactatc
      481 gtaaagattg acaaaatcta tcatttcacc gccgaattcg actggctcca tttgttctaa
      541 caattgacgt ttgccatata atacaccgat tcctgtcggc ccgcacattt tatgcccgct
      601 aaaggcgaaa aaatctgcat ccaacgcttg tacatcaact gtcatatgcg gtgctgcttg
      661 ggcaccgact ggactatcct caactacaac agcctgttca accctcagcc gtttcaagca
      721 caacaaataa ccttctggat ctggtttgcc gacctgaaga tcatcgatcg tcacaaagga
      781 atcaattttt tccttaatca tcggatgtgc ttcaaaaaaa tagtctagct cccgcagttc
      841 agaattgaga ctactccgat tttccagtgc tgctgatccg ctgctcaaaa tgttcagcat
      901 ccactggtct cccaaccgct tgacttatcc cttcataaaa ggacagttct gctgccgtcc
      961 caacaccttg ttgatagtct gcaaaaggaa ttttatagcc gaactgctcc agtaaatagg
     1021 cttcagtaaa tagtgatgaa tactctcgta aaacatccat tcactgtcaa tcatcgtccc
     1081 atcaaaatca aaaataagtg ctttggtcat cttctttccc ccttacccaa aacaagcaaa
     1141 agctgtgtcc tgctctctct acagtatcag cataaaaact gccgtctgaa aagtcaaagg
     1201 tgaaagaaat gtggcgctat aatttctagg actgatagta aatcattcta tcagtccttt
     1261 tgagcataaa aaaacaaaac atacagaagc tccgttagaa ttgaatcacc acaacacaat
     1321 caaaggagcg ttctgtatgc ctgatcttca tcttatcaaa ctattactag gaatcaaaga
     1381 tggaaacatt caaatcactc atgtcgagaa gaaaaaaata cattcaatca acagtatcgt
     1441 tgtttctgcc acgctcatta aaacgattca gccctgtcct cagtgtaaac aggtgaatca
     1501 acatgggctg atcgttaaga acggaaagaa aaaatcgctg gtccttctca ataagtgtgc
     1561 gaatcaacgg acttatcttg ctttggcaaa gcaacggtac ctctgtcgtg gctgtcacac
     1621 ctattttacg gcgaatacct acattgttga aaaaaactgt tttatcgcca aacaagtaca
     1681 ttataaaata ttggaggaat tggctgaaaa acaagcaatg acgacgattt ctaaacattg
     1741 cggcgtttct tggtcaaccg tttcgcgaac actcgcttct ctgctcccca tgacaaacgt
     1801 gaaaataaat tggttgcctc gctgttcatt ggtagatgaa tttcgttcat tgaaaaatca
     1861 agtcggtccc tattcttttt cttgtatgga tggcgatact gggaaattac tggatattct
     1921 tccttcacgt aagaagaaag atctcatttc ttattttatg caatttgaac gacgtgcacg
     1981 tttaaacgta aaaatactcg taacagatat gaacgcctct tatgctttgc tgataaaaga
     2041 gtgctttcct catgccaaac tcgttgtgga tcgctttcac atcgtcaagc acctcattcg
     2101 cagttttaag gatatttgac tatgagtcat gaaaagcttc gaccgaaacg atccaataca
     2161 agccaaacac tatcgacaag tgaaggcttt atctcgactt ctgatcaaac gacaggacac
     2221 gcttgtttat gataaatgga caaaatggcg taattttggt tgggcctatt taaccgaaag
     2281 tgaagtcgta gaacgtctac tttctacttc tgatgaacta cgaatagctt atgcttacta
     2341 tcaagaaatt cttcaagctt tttatgacaa agaagcggac atcttctttc agcttgtaaa
     2401 aacaatgccc aagtcggttc caagagaact gcatcatata aaaaaagcgt ttataaacta
     2461 tgaatccggc attcgtttag ccttagagtt gccttactca aatggcaaaa tagaaaatct
     2521 gcatacccac atcaaagcat tgaaacgaat agcttacggg ttaggatgag aaccagaatt
     2581 ttcttaatca atcatctgat cacttattaa acataaaaaa acatctaaag cagaaaatca
     2641 attctacctt agatgttctt atatcactct atcagtccta tttgacaaag agccagaaat
     2701 gtttgccgac tatacactag ctttgttgtg caaaatgctg agtatcaatt tccacaggga
     2761 ttgctagaat atctttcagc tgaatgtata aggcatcgct aattttctct tcccgatcgt
     2821 tcaaccgatc cactaaaatt tctttgccct ccagcgtttg aaaaacatac cgttgaatgt
     2881 tgcctaaaat aaatgattcc ttccatagaa ccggaatctt aaccccctga ggaaccggtt
     2941 caaattgaat cagctccggt cttaaataat aaatgcagcg attgccctta gcggaaaact
     3001 cgtgggtata ctgattcagc tcataccatt cgaaacgatt atagttgccg ataaattccg
     3061 caacaaatcg gctctgagga ttctgataca attccttcga ggtggaaacc tgagccaccc
     3121 gcccagcttc catcacaaaa atccgatcac tgattcgcat cgcctcttct tggtcatgag
     3181 tcacaaaaat catcgtcatc cctagcgatt gctggatagc gcgcaaatcc cgctgcagct
     3241 gttttctaat tcgggcatcc aaagcactta atggctcatc cagcagcagg actttcggtt
     3301 cagtgaccaa tgagcgagcc agtgccaccc gttgtcgttg accgccagaa agaaactgga
     3361 catttttatc tgcctgctcc gtcatatcca ccaatgatag catctcaaaa accttttcct
     3421 gaatcacctc aggactcagc ttctttacct tcaaaccaaa ggctacattt tcaaaaacag
     3481 tcatagttgg aaacaatgca tactgttgaa aaacaaagcc gatattgcga tccttcgtag
     3541 gcttatcggc aatattctca ttgtccagca caatctcacc gctctttggt tgttccaatc
     3601 ccgtgataca gcgcagcaac gtagactttc cgcacccgct gggacctagt aaagtaacca
     3661 gctcaccctt ttcaatctga atttcatcaa tagctaaaat ctgtttttca tcaaaaaaca
     3721 gattgagatc atgtatttct aaaaacattt tccgtcacaa ctcctttaag ggcttctttt
     3781 tcgactgctt ctcttccctc gtctgcttaa gggacagctg ttgcttttgt cgataagtca
     3841 gcataaaggt agttaaagca attcccacca taaacagcaa atacaaaacc atcacagcgc
     3901 tagccaaatg gccgttttcg ttcatccgcc gcagcatata gatgcgtacc gtctgaaatc
     3961 gaccgccaat caacaggttc gtcagcatgt actccccaaa aaccgaagaa aaaatcatca
     4021 gagaaactag ggtcacaccg atacggatgt ttggaaacag cacctggata ataatcgtag
     4081 acatcggggc gcccaaggtt tctgctgcat ccactaattc cttcagatta attaagcgca
     4141 tctggttgtt aagactaaga tagacaatcg gcaacgccgt aataaacaat gcacctacca
     4201 acactacaaa cattgggata ccagttttag catacgttcg aagcaaagcc gttaccaaaa
     4261 tcactcctgg taaagcatac ggcagcagga gcagcttttc cagccaacga ttcaaacgag
     4321 ggaaatacaa atgaatccaa ataatggtgg gaatcatcaa caacagaatc gtcaccaaga
     4381 ccacacctgc taataaaagt gagcgaccca ccgcagcaat aaattccctg tccgtcagca
     4441 gctgttggaa ccattgcaag gtaaaatcac taggtaaaag cgattttgac catttagtgg
     4501 agctggcata caaaatcgtt ccaataatcg gcagcaagta atacaggaca aagcagaaca
     4561 aaatcggttt tattaacttt ttcacgtctg caccaccttg cctttctttt gaataatcaa
     4621 cattgccgtt accagcagga cacctaaagc gactgccaac gcactgccta aattaggctt
     4681 ggcgaacaca tctcccgaca ctaaagccgc aattcgaatc gttaaaagat ttacattact
     4741 gctggtcagg gcatacgctg tttcataggt ccccatgcca ttagcaaata agatgacaaa
     4801 ggtgctggca atcgatggtc gcaaaaaagg caaagccacc ttgaaccaag catacgctga
     4861 tgaagcgccc agcaattgtg cggactccag ccattccttt ttcacttcct tcatgctggg
     4921 atacaaaaac aagaccccta acggaatttg gaaataggta tacgtgatgg tcaatcccca
     4981 ccaagaatac aaattgaagt ccgctagaaa tggcaaaatc attttcagca cacctacatt
     5041 ccccaacaaa ataataaaag agaatgccag cggaatccca gcaaaattag ctgccaaatt
     5101 ggaaacaaag gtgattttct cctgcaattt agtcgataga ttcattaagc ataaacataa
     5161 aataaacgtc ccgattaacc caatcacact ggagctgaca gcaatcagta cgctgttaac
     5221 aaaggcctgt gtgtaatatt tgttgctgaa aatatcactg taattggcca atgtccactg
     5281 gttagttaaa tcactgtgca agctgcacag aaccataaaa agcagcggca gcagtaaaaa
     5341 tgcacagaag ataaaggagg caggaaccag aaccaagcgt tttttcatct ctttcatccc
     5401 ctcatcaatt cttgaatgtc ctgcattttc tctgacggtg caattcctaa taaccagcaa
     5461 aacaatggag ccgccagcag ctgtggtaca gtttgttcct tcagcttttg gcgtttattt
     5521 gaaatcaaat aaaaaggaac ttcacgatgt gcagccaagc tgccgccatg cagtccgtgc
     5581 gcatccatcc cgtgatccgc agtcaccacg acttgataac ctttttgcag ccattcagga
     5641 aggtaaatag ccaataatct gtccgcctga ttaaccgctg ctgcatattc atgagaatcg
     5701 gcggtaaatt tgtgaccgac atcatcaata ttcatcgaat gtacgaccaa aaagtctggc
     5761 tgatactgat tcactaaata atgggcatcc gcaaataaat gcgaatcagg gtaagtgtcc
     5821 tcactataaa agatgccatg ctgaatcagt cgctcctcct gatgctggat gcgatcaacc
     5881 aaacgattat agggggcttg attgtacaac tcgctgtacc agtggtaagc ggcggctcct
     5941 gtttttccgc cagcttcctt aactaattca aacagcgacc actctttgga tcgctgagca
     6001 tggtaattac tatagatgcc attttcatag gctggaacgc cggtcatcag tacttcgtat
     6061 aacggacgtg aattgctggg cagctcactt tttaccttca ataagcttgc ttgctggtgc
     6121 tcaactaaat gattcatgaa cccaagctgg gcgacggctg tgtcgtagcg acagccgtct
     6181 agtataacta caatcagttt gtcaagtcta ctttgcatag gtcagtacct ccgcctgcca
     6241 aagctctgga agctctacag aggtcttgtt ccaagcttca ttgtcttcaa ctggcaccgc
     6301 cgcttcataa gcatcctccg gcagtaattt ttcagctact tctttaggca gctctacctt
     6361 gcgaataggt cgcgcatagc cctttgccaa attgatttgc ccttcatcag ataaaatata
     6421 ttcccgtgct agctttgccg cattgggatg aggggcattt ttattgatta aggttgtata
     6481 cccgctaatg atggagccgt ctgttggcac agttacctca aaacgctcgg agtcaatttg
     6541 gtcacgataa tttagtccat tgaagtccca aacaactgcc acatcgattt cacctttttc
     6601 aagatttgct acgctggcat caacggtgga caaacggcct ttttctgcca tttttttgta
     6661 gtaattcaga cctggctgaa tatccttttc gtcacctccg ttggcaatcg ccgcagccaa
     6721 tacagcaaat tgtgcttggt tggcaatcgt cacatccccc acgctaactt gatagtcacc
     6781 ctcttgcagc tccttccagc tagtcggtgc ttgcttcaca ttctctttat cggtgataaa
     6841 ggaaatggtt cccgtatagc ttaacaacca atccccatca tcatcctttg cccaatccgg
     6901 aatttcctcc caatagttgg tcttataagg taaggttaac tctttgcttt cagccaacgg
     6961 accgaagctg ataccgacat caccaatatc ggcggttcca ttttttcctt ctgattcaaa
     7021 cttagccaat tcttctgcac tggacatatc cgtatccacg tgacttaagc cgtattcttt
     7081 ttcaaggtcg ttccaagtgc ctacccaatt tgcccaagta tccggcattc ccaagctggc
     7141 aatctctcct tcttccttgg ctttttctat aatcgtatct aaagagtcgt ctgccgcagc
     7201 cgattgcttc gttgcttcac ctgagccgca gcctgtcagc aggatactta ctgttgctgc
     7261 acaaaataaa ccgatagtca tgttacgaat tttttcattc attttcattc attcttctcc
     7321 cctttcgata attccattct aacaaagctt tgtaatgaga gaatcagttt cttgaaaagg
     7381 tttcatgaac aatgtaaagg ttgtatatgg tttgtaaatt tcgacgaatt cccgttaacc
     7441 ttctaaagct tttgcggtat gatagtagaa aaaaggaggc tccctatgca gtattatgat
     7501 cagcatctcc acacgtattt ttcctttgat tcagaggaaa aatttgaaaa ctatttagcc
     7561 tatcagccgg agtttttcgt ttctacggat cactttgatt taaacaatcc atctaccgga
     7621 aaagatgaca ttccggatta tccagcctat ataaacaaac tggactcttt aaaagaaacc
     7681 tattccacca aatttctgcg aggtatcgag attggcgttg ttccaggaca agaagagcgg
     7741 atcgtggatt acctcgctca gcatccttat gatttaaagc tggtaagtat tcatcaaaat
     7801 ggccgctttg attatatgga cgatgtggtt ttaaccaaag atcccattca aactaccaag
     7861 gaatattttg atcagatgtt gcaggtcttg acggattttc cagaaggaga tattttgact
     7921 cactttgatt atggattacg ccgcttcacc ttcacagcgg aagaattcgc ggagcatttt
     7981 gaggctcagc tgctaacgat tttcaaaaag gttgttgaac gagaattggc gatggagtta
     8041 aatgccaaga gcttcttgaa atacggcaac gaagaccttt accgttatgc cgttcctttg
     8101 tatcaaagtg tcggcggaaa gctctttacc ttaggctcgg atgctcatgt tgccgaagat
     8161 tatcagctgg gctttaaaaa aatgagtcag ctgttggcag actgtcaggt agaaaagttg
     8221 ctagtcattc aaggaaaaga acgccgcctg acgccgctgc cgattttgtg attcctatat
     8281 aatagaagaa agtggtgaaa aaaatgagca acctgtggga ctacaatcaa gaggcaccca
     8341 tccattattt aattgcgcga cattgggatg cgttgaaaat tgaggcggtt tgccgatcct
     8401 tgttagcagc cgttccaaaa caacaactgg aaaatttttt ggtggctgac agcttgcagc
     8461 gggaaaaagt tcaggcttat tttgcagcct ttaaagatca gcctttggaa tatttgcatg
     8521 cgcagtttca tcttttttat caagtagccg ccccagatga ctacaacgat ttacgaggac
     8581 agctgcaatt gacctttcaa gcggatgaaa ccgcctatac cgttttattg ggaatggctc
     8641 gccttggtga tcaggcgaag gtcgagtggc ggattttcga tatttgatgg caggatcaag
     8701 gcgtttcagt tgcaaacctt cctctctttc aattagaaaa accgcagcag gaagaagttt
     8761 gtgtgctggt caccgatgct ggtgaaattc gtctccgctt atttcctgac caagctcccc
     8821 tggctgtagc caactggcgc tccctagctg agcaaggcgt ttatgacaac acacctttcg
     8881 gctcgtgtca ttaaggaatt tgttattcaa ggtggcgctt tggatggttc tggtgatgaa
     8941 gctgtttcca gctatgaggg ctttttcgct gatgaagtgc atcgcggact ttatcatttt
     9001 aatggcgcct tggccttagg ggaccacggc ccccacacca acggcaacca gttttttatt
     9061 gtgcaaaaca caaaggcgca ggctgacctg ctaatgtaaa gcaagcatac ttggagcttg
     9121 gaggactacc ggagctggat ggtcgttata ctgtttttgg acaggtatac caagggttag
     9181 aaatcgtagc agcgatcgca gctcagccga caaacagtga agatgtaccg attgagccag
     9241 tgttcattcg acaaattaac ttcgaaaaaa catcgtaaaa agacactcac tgacttcagc
     9301 aaaagctcta ttcttgagct ggctgcagct agtgagtgtc tttcatttat ccataagata
     9361 aagcttggat tcaataaata aataaatgtt ttggttaaag ctgtgaccag caaatgagga
     9421 accgtgcgag ccagttgatc tgccaatgca aacatttctt taggtgaagg cgctggcgtg
     9481 tctgccaacg gatactccag tcccaatgct tggtaaccgt gcataccata gcagtggtac
     9541 ggtagtaatt ctaacggtac gaaccgatca agatctgcca caaaacaagc aatcgtcagc
     9601 agctcggtca ggttcatatt gatacctgga accacaggaa ctcgaattcg cacttcgcag
     9661 ttaggatact caattaaaaa gcgacgaata ttcttcaaaa tttgttggtt atctaagccg
     9721 gtccattgtt tgtggagctt gctggtaaac atttttacat ccacaaacaa tgtatccaca
     9781 tagggagcta ccttttggat ttcattataa gcaccacaaa agctggtttc cagtacggta
     9841 ttgattccaa tgtatttcga tttttgcaaa atttctttag caaaatccgc ctgaaccagt
     9901 gcctcgcctc cgctaatggt cactccgcca tccgagtgaa agtaaaaaac agcatccttc
     9961 tcaatctcat ccattacctc ttcagcggtc atggtctggc caaatccttt ttcaatctgc
    10021 ttgctttgtg attcgggaat cgaacaccag gcacagcgta atgggcagcc ctttaaataa
    10081 acaacggtcc gtaaaccagc accatcatgt agagaaccct gttctattct taatactgtt
    10141 ccttccatat agatttttga attacacact agtttaaact aaaacacaca aggaaaaact
    10201 taccatttaa tcataaaatt agctcatata aatatactag gatgtaaaat agactggtca
    10261 aaatctggtc aaaaaagagg actaatacag gtagtcctct tttttgttat tttaatattc
    10321 taaatcattt tttattggtt tattccctta ttcgatgtag actcaaatca ctatatcact
    10381 agatatcaaa cggcgtcata cggaatcaaa aacatcaaat ttgacctgtt aaatggtcat
    10441 tgaataacat tcaaaatcaa taaaacggag tcagacacgg agtcaaaaac gctgagagta
    10501 aaaaatatat acagccttgt aggtaaagta ccacagggct gtatattttt tatttcagat
    10561 taccttaaat gattccattt actttttatc tcattttttc ctactagtta gtatcaaata
    10621 ctctcctatt ttctcaataa actatttttg gatacatggt atctgaaaca gctaaaacct
    10681 tgtcattaca gcaaaaaatc acgttatcca gactgtagac aaagtccatt ttatggatga
    10741 tgtctacagt cttttctttt ataatagatt tatgaaaacg aa
//
LOCUS       DACEUF010000084        10646 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.088, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000084 DACEUF010000000
VERSION     DACEUF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.088"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            23..646
                     /locus_tag="IXG20_002191"
     CDS             23..646
                     /locus_tag="IXG20_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610371.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IXG20_002192"
     CDS             1096..2145
                     /locus_tag="IXG20_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1610372.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IXG20_002193"
     CDS             2142..3023
                     /locus_tag="IXG20_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1610373.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IXG20_002194"
     CDS             3026..3796
                     /locus_tag="IXG20_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1610374.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IXG20_002195"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IXG20_002195"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ1610375.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IXG20_002196"
     CDS             5107..6270
                     /locus_tag="IXG20_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1610376.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IXG20_002197"
     CDS             6387..6797
                     /locus_tag="IXG20_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1610377.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IXG20_002198"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IXG20_002198"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1610378.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IXG20_002199"
     CDS             9337..9654
                     /locus_tag="IXG20_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610379.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IXG20_002200"
     CDS             9814..10050
                     /locus_tag="IXG20_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1610380.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IXG20_002201"
     CDS             10047..10445
                     /locus_tag="IXG20_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1610381.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3715 a         1773 c         2253 g         2905 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttat
//
LOCUS       DACEUF010000085        10500 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.089, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000085 DACEUF010000000
VERSION     DACEUF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.089"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="IXG20_002202"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="IXG20_002202"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ1610382.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="IXG20_002203"
     CDS             1085..2005
                     /locus_tag="IXG20_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1610383.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="IXG20_002204"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="IXG20_002204"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ1610384.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="IXG20_002205"
     CDS             3311..4789
                     /locus_tag="IXG20_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1610385.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5056..7116
                     /locus_tag="IXG20_002206"
     CDS             5056..7116
                     /locus_tag="IXG20_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610386.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7199..7768
                     /locus_tag="IXG20_002207"
     CDS             7199..7768
                     /locus_tag="IXG20_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1610387.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7943..8749)
                     /locus_tag="IXG20_002208"
     CDS             complement(7943..8749)
                     /locus_tag="IXG20_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610388.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8887..10401
                     /locus_tag="IXG20_002209"
     CDS             8887..10401
                     /locus_tag="IXG20_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ1610389.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3721 a         1751 c         2070 g         2958 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat tattccgatt
     4921 ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct tcttttgatt
     4981 ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga caagagtagg
     5041 gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa acacgcttgt
     5101 cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat gatgcgcacg
     5161 atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc aacgacacaa
     5221 accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat cactattact
     5281 catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc ctataataaa
     5341 gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac attatggaca
     5401 tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga tcctacaaaa
     5461 ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga tataaaggac
     5521 ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt tgaaagaaag
     5581 aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga aaggctgaag
     5641 aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg gaatatcgtg
     5701 gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc aattgcttta
     5761 aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc tatcaaagga
     5821 aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt tgaatatgta
     5881 acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg gacagtagat
     5941 actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa aacatcaaat
     6001 gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc gactgtcatc
     6061 actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca tatgagtagt
     6121 gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt gccaaatata
     6181 gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg gatgcaagca
     6241 aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca ctcagaactg
     6301 atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta tataccagac
     6361 aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat aaacaaagaa
     6421 gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa ggatgtatcg
     6481 gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac aggtgatttt
     6541 acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc tcagtcacta
     6601 ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag acaagaagaa
     6661 agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg gaaagtaaaa
     6721 caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc taataaaacg
     6781 ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga aattttagag
     6841 cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga agatcagaag
     6901 gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga ttccaccgat
     6961 gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc tggtggaggt
     7021 attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg ggtatacgct
     7081 aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc tgttatagtc
     7141 aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg atagaaaaat
     7201 gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc aacaaaatgt
     7261 tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag aagatgaatc
     7321 tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg aagcacttga
     7381 gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga tgttttttgt
     7441 agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa aatcaagaga
     7501 gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag gaaaagggct
     7561 cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata cggacggacc
     7621 aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata aaaaaatggg
     7681 ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg aaaaagttat
     7741 gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc agtattggga
     7801 taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact atcggataac
     7861 taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg tcactgcccc
     7921 ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa ttcttgatga
     7981 acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg aaaacaactg
     8041 ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg tctaagtaac
     8101 cattttggat gattgtttta tctgttttac catctgcatt ttgactttca atgattgtcc
     8161 gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc acactattga
     8221 tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct gctgaaaatt
     8281 ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca actgctagta
     8341 ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca agggaaaagc
     8401 cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac attgaaaaat
     8461 agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa gataacaaaa
     8521 tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa taagggattt
     8581 caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg ttccagacct
     8641 gatccactag cataataacg acgatgatac gtaataaaat atagattccg tttgtgattc
     8701 tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata aaataactcc
     8761 ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat atgacttaaa
     8821 gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga gaaaaggggg
     8881 atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct tgctatgagc
     8941 gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa ggaatctgcc
     9001 gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat gaaagaatct
     9061 tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat cgaagcaaaa
     9121 gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg aaacacatct
     9181 aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga aggaatcaaa
     9241 gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac gaatgataaa
     9301 gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct agatcaaaac
     9361 ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag gacacatcgt
     9421 ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa taacgtccat
     9481 gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa aaatggggaa
     9541 acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt caccggaaaa
     9601 gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga aaaatatcgt
     9661 cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga tggtatcacc
     9721 atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat tcatccaact
     9781 gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg agcaatcttg
     9841 gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa agtatctgca
     9901 gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg cgtgcgtgat
     9961 cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg aaagacaatg
    10021 gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac agttgatgga
    10081 tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac agccatggaa
    10141 catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat ccattacacg
    10201 atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa ccagccaatt
    10261 agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa ccggatcggt
    10321 ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca agcagctcga
    10381 tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga aatcagcgtt
    10441 ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt tgtgaaaaag
//
LOCUS       DACEUF010000086        10408 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.090, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000086 DACEUF010000000
VERSION     DACEUF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.090"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(<1..71)
                     /locus_tag="IXG20_002210"
     CDS             complement(<1..71)
                     /locus_tag="IXG20_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1610390.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEA"
     gene            295..1086
                     /locus_tag="IXG20_002211"
     CDS             295..1086
                     /locus_tag="IXG20_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1610391.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1052..2005
                     /locus_tag="IXG20_002212"
     CDS             1052..2005
                     /locus_tag="IXG20_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAQ1610392.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2403..3377)
                     /locus_tag="IXG20_002213"
     CDS             complement(2403..3377)
                     /locus_tag="IXG20_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1610393.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3492..3926
                     /locus_tag="IXG20_002214"
     CDS             3492..3926
                     /locus_tag="IXG20_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1610394.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4051..5071)
                     /locus_tag="IXG20_002215"
                     /pseudo
     CDS             complement(4051..5071)
                     /locus_tag="IXG20_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
     gene            5463..5885
                     /gene="arsC"
                     /locus_tag="IXG20_002216"
     CDS             5463..5885
                     /gene="arsC"
                     /locus_tag="IXG20_002216"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAQ1610395.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDL
                     KDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
     gene            complement(6165..8087)
                     /locus_tag="IXG20_002217"
     CDS             complement(6165..8087)
                     /locus_tag="IXG20_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ1610396.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(8174..10114)
                     /locus_tag="IXG20_002218"
     CDS             complement(8174..10114)
                     /locus_tag="IXG20_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1610397.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
BASE COUNT         3194 a         2031 c         1783 g         3400 t
ORIGIN      
        1 gcttcttctt tattttttac ttctaccgct aattgaatca aactaggaac gaacatctct
       61 ttatatttca ttccaacctc ctcctttttg attaagtttg ttaatttatt gttctactaa
      121 gttcatcttt gttttacaag aacattatat ttctgttagt ttgttttgtc aacgctttca
      181 aaagaaattt attttgttaa atttgatcta cttgaaaata aaatcgttta tctatataat
      241 taagaagaac aaagtcgaac aaaaattgac ttttatgtta aaggagtaat tcatatgctg
      301 aaaaaagaaa gacaagaaca aatcattaca ttattagaga aagattttta tttaactaat
      361 tcagaaatcg ctaacaaatt aggcgtatca gaaatgacca tacgccgaga tgtcactgaa
      421 ttagcaaaaa aaaataaagt aaaaaagcta tatggtggcg ttgaacgaaa ggaaccttat
      481 aatcgagaac tatcgactca agaaaagatt gatacgaatg tagccgaaaa aaaatatatt
      541 ggtcaagtca tgaacagtat gattcccgat aattctacta tttatgtcgg tgctggaaca
      601 acgatcctat atgcacttcc tttcattcaa aagaaaaatc tatttgtaat tacaaacagt
      661 ttgattgctt tcaactattt aaagaatcat acaagttatc ggattttact tactggtggc
      721 gaattttctc ctattacaga agagttcatt ggggaagttg cagaaagatc ttttgaaagt
      781 ttgaatgtcg atatctcttt cgctgcaaca aacggcatat ttgataacaa tgtgacaacc
      841 gctcaattta ttgagggaaa tattcagaac gcagcgctca atgcagcaaa aatcaaatgc
      901 attgttgcag attattcaaa attaaataaa tcagacgtct atacttttag aaaattaacc
      961 gattttgatt atctaataac cgataatcat ttatcagaac aggatttcaa tcattattca
     1021 aaatacttaa caatattaaa ggagcccaaa aatgatactg acaatcacga tgaacccatc
     1081 cattgattcc atttatttta caaaacactt tgaattaggt acgatgacac gctttgattc
     1141 tccggtacgc tatgtaggcg gcaagggaat caattgcgga cgagcttctg ctctactagg
     1201 ttcatcagtt attttaacag ggtttttggg cggttatttt ggcagtatga ttcaagatat
     1261 gctgaaaaat gaaaatttgt ttgatttgca ttttttacct attgcagaag aatcaagagg
     1321 tgccgtcacg atcatgcatg atgatgaaac gcagacagag ctagtggaag ctggtcctga
     1381 aatagcagat actactatca aaaaattaat gaatgatatc aaactaatca gtaaaaataa
     1441 ttacctttca gtcattacaa taaatggttc tgttaacaat ccagatccct ttttgtatgc
     1501 taaattatta caatatatca aagaacaaat agatccttcc acctatatat taatggatat
     1561 atctggacaa catctccgtg aagtcttagc aagtaagtat aaaccgatgt tcatcaaacc
     1621 taatatccat gagttttcag atatcatcca tcaaaaaatt actcaaaaaa gcgacataaa
     1681 acaccatcta aaaacaaatc cactatttaa agatatcgat gttatcatga tttcttgcgg
     1741 ctcgtatggt gcagtagtca aagtaaaaga agaattttgg aatgttgaga tccctaagat
     1801 tcctatcatt aatacaacag gctctgggga tgccactgtt ggaggttttg ctcattcatt
     1861 agaacagaat tatcctttaa aagaatgtgt aaggtacgca atggcttgcg gcatgtcgaa
     1921 tgcacagcaa tcagaagtcg ggaaagttat tccagctgat gtaaaaaatt tagcaaaaag
     1981 cattaaaatc acagaaatga aataaatagt acaaagtaaa taattgttta tatgaaatga
     2041 gacacaaaga gactgtgaca aaaaacgtgt gtaccgaccc ccaaaagtta gattttttgg
     2101 tctaactttt gggagtcgat acattgtcac agtccttttt tcgaataaac ggtgttctcc
     2161 tgccaactcc tcggtcacaa gtcacaatat tcaacaaaca tgagaagcta ttttttgaaa
     2221 ttgctccttg tgactcagag ttaaacggca ggctcacgac ctcttggtat aaacggtgtt
     2281 caaaagcttg catctacgct aatgagtcca accaagtcaa aaatgaggag cttttttggc
     2341 tgtccttatt acttgatgca gggcagtttt ttcataatct cttttagatt aaatcctttt
     2401 ggttacacaa ggttctcttc aaagaatgtt gccatttttt caaatggaat gatgtctgtc
     2461 tggtcgtata gatctgtatg ggatgctcct ggaatgatca tgagttcctt gttgtcacct
     2521 tttagttctt tgaaagcgtc ctccccaaag taacgtgaat gagctttttc cccgtgaatg
     2581 actaagactg catgacggat ttctggaata taatgcaata gttttgcatt cataagtgac
     2641 aaccctgatg tcctattcca accatcattg gagtttaaag aacggacatg atatccgcgg
     2701 tctgttttat aatacgcatg atagtctttg acaaattgag gagcatcttc aggcaacgga
     2761 tcaaccacac ctccagcacg tgcgtagtcc ccttgtttga aatcaatgat gcgttgtgca
     2821 tttaattctt cacgcatttc atatcgggca ttttcatcca taacatcgaa gtagccattt
     2881 gctgttactc gagtcatgtc atacattgtg gaaacaactg ttgctttgac ccgcgtatcg
     2941 atagctgctg cattcaatgc catacctccc catccacaaa taccaatgat tccgattctc
     3001 tcagggttta catttttttg tacagataaa aagtctactg ccgcttggaa atcttccgta
     3061 ttgatatcag gcgaagcaac atatcgaggt actcctccgc tttctcctgt aaacgatgga
     3121 tcaaacgcaa tcgttaaaaa gcctctttca gccatcgtct gcgcatatag accagacgat
     3181 tgttccttca ctgcaccaaa tggcccgctc acagcgattg ccggcaattt cttttcactt
     3241 acttcttttg gttcatataa atcagcgact aatgtgatac caaaacgatt cgggaagctc
     3301 acctttcgat gattgacttg attgcttttt ggaaatactt tgtcccattc ttgtgtgatg
     3361 tttaattctt tattcatatt ctagactctc ctttgatcaa aattgacatt tgtttttgaa
     3421 tgtattatta tgataaaact taaacttgac tttaggtcaa ggtaaaaatt ttattttagg
     3481 aggatttttt tatgtattcc attggacaag tgtctgaaat gtttggattg cctgtttcta
     3541 ctttacgtta ttacgacaaa gagggattat ttcctgatat ccagcgtgtt tctggtatta
     3601 gaaaattttc agagaatgag ttagaggctt taagagtcat tgactgtttg aaaaaatctg
     3661 gtttagaaat aaaagacatt aagcaattca tggaatggag caaagaaggc agtaagacat
     3721 ttgaggttcg taaagaacta tttgaaaaac aaaaagaagt agtagaaaaa gaaatagcaa
     3781 aattagaaag agtactcgct atgctaaact acaaaagctg gtactatgaa gaagcaataa
     3841 aagccggcaa tgaagaagcc gtattgacaa tgattcctga tgatttaccc caacatgtaa
     3901 aagaagctta tgtacattct cattgaacaa acggataact cactctcctt ctctatgaac
     3961 ttcatgaaaa catgaataaa cacctctaac tgattgttag aagtgtttat tttttcactc
     4021 aagtatcctg cacttacaaa aatatccttt ttaccgccat cctaattctg gagcgatatc
     4081 ttttacgatt gtttcaagca agtgcatgtt atattctacc cccaaagtat taggaatcgt
     4141 gacaagtact gtgtctgctt ctttgattgc ttcatcttca gctagctcct tgattaattg
     4201 atcaggttca ccagcaaaag tttttccaaa aatcgttggg tttcggtgat aagcaagata
     4261 gccgacagca tcttgttgcg gactaccgtc ttgtccaaat aagcgtcgat ccaaatcagt
     4321 ggtgattggc tgaatcgagc gagtaactaa tgttcttggt tcaaaatcat gacctgcttg
     4381 cttccacgct tctttatacg cacgtaattg gtttgcttgt tggatatgga atggctcgtg
     4441 ggattcgtaa ttgactaatg tagatgattg cagattcatt ccatgttgtg ctgcccagat
     4501 tgccgtttgc tgcgaaccag ctccccacca gattctttgt cgtaatcctt ctgaataagg
     4561 ttctatccgt aattttcctg atccttgtgg gaacattggt tgaggatttg gttcttcaaa
     4621 tggttttcct tcaagtaatt ccaagaattc aagggtgcgt tcccgtgtta cctctttgat
     4681 ttcttcttca gtaatcataa ccaaagtatt tccagccatc tagaacttgt tcaggtgaac
     4741 cacgccccac tcctagttgc actcttcctt gcgtgatgat atctgtcaat ccggcatttt
     4801 ctgccatata ataagggttt tcatatcgca tatcaataag tcctgtaccg atttcaatct
     4861 tacttgtctt tgcgccgatt gccgctagca aaggaaacgg cgacccaatc tgaggggcaa
     4921 aatgatgtac gcggaaaaac gctccgtcca ctccgatttt ttctgcttcc accgctaaat
     4981 caatcgattg caaataagca tcttgagctg atttcacaag cgatccttgc tccatccaat
     5041 gtccaaagct taaaaagccg atttttttca aggtaatgac ctcctttaaa cctacaaaaa
     5101 tggaaggttt tttattttta atatgaactg attctactac tttttttctt aaaagacgaa
     5161 taattgttct cgcctcacaa tcaagaaatg tatcggctta ctacacccct actttcttca
     5221 tcatctgccg tgtcttaatc tcttttaaca gtctgttaca tcgttgattt ttgactcaaa
     5281 atgattgttt ctaacatgta tttatggtaa aaaagacaag attagttctt tgtacacatc
     5341 gatttatacg ctgaacaaaa agaaatatat aaactaagaa acccattgtt ttttcgctcc
     5401 atcacataga gtttacttta gataaacagt gacatttact aatccataac ggaggaaatc
     5461 aaatgaaaaa aatctatttc ttatgtacgg gcaattcatg ccgcagtcaa atagcagaag
     5521 gctacgcgcg taaatacttg tctccttcaa tgtttgagat aagaagtgcg gggatcgaga
     5581 cacatggctt gaatccaaga gctataaaaa tcatggcaga agacggcatc gatatttcca
     5641 aacagacgtc tgatttgatt gatagagatt actttactca ggcagacttg atcattacac
     5701 tttgtggtga tgccaaagac aagtgccctg ccgttccaaa caatcacgaa caccttcact
     5761 gggacctaaa agatcctgca caagctgtcg ggagcgaaga agaaatatta acggaattca
     5821 gaaaagtcag agaaggaatc aaaagaaaag ttttagactt aaatcaacgt acatcgatcg
     5881 tataaagaag tattcaaaat gatttttcaa gcactaagaa acaatgttaa aaaaatagct
     5941 ttccatattt tacaaaattg tgccttattg ccgacaaatt gtctcttgaa caccgtttac
     6001 cagtagtcaa gacatcgtaa gcaactataa aaaattataa taaactcaaa ataaaaggat
     6061 gtgccacaac tttggccaca tcctcttttt ttgtatttag ttttctactt ccaaataaat
     6121 agatagaatt attataaata taaaggcagt tttttaatcg ctacttattc ataactagat
     6181 aacaacgtta agatttctag cggatgattg gattgcatca actgagccat ttgttctggc
     6241 tgcttttcta gatagtgaat aacagaaaat atttttttga actctgcatg tttcaaatca
     6301 tttttcttta ttgctaatag cagtacgata cgtatctgtt ccccgtgcca taggactggt
     6361 tctttcattg tagcaataga aatcatcgaa tgcttcacat acagcggatt accgtgaggg
     6421 attgttacaa atggtgctac tctagtactg gagtgactgt ctctttccaa gacagaatcg
     6481 atgaattccg gctccacata atgatgttcc actaagacgt ttcccataaa gtttaaaata
     6541 tcggctgggt ctgtccgttc aagttgagga aagatcagaa atggatgagt aaagcttgct
     6601 agatcaaatg tttttttctt cttgataggc acatgctcca gcatatattt tgtcaaatag
     6661 gctagatcag tttctgtgat cattggtgag atctcgatta ctggaatgct tatatgttta
     6721 aatggtaacg tagccaagat aaaatcaaca gagggaaaat cttgtctctt taattctggc
     6781 ttatattcaa ctagatccat aatcgttaat tctggaagtt ctctgttcag ccgtgcttcg
     6841 agaaaagtag ccatagcttt tgaatattca ctaactaaaa ccgtttgata tcttctactt
     6901 ttctgatgtt tgtgtcgctc gatcgctgct tggaaatgga tcgttaaata agctgtctcc
     6961 tcttgtggaa cataaaaatt ttccttttca aaataatctt ctaaaattaa ctgtaaaact
     7021 aagaaaagtt gtgtgtacgt actaaacaca tttttcgtca atggattaga aatctgcaaa
     7081 ccataatgga gtcgaaaata agttgaagat aaatgagacc ttaaattttg ttttagtagc
     7141 tgatcttctt ttaatgaata gcctagaaac tgttccactt gttcgatcaa atgatcagca
     7201 agtgcgctag attccttttg aaaacgtgtg gctgcttgtg aatttgtaaa aagattggcg
     7261 atacgcaaag ccagataatt gatttcatca ggagaaaatt caattggata gatggtggct
     7321 agttttgcta atatttgact acttaacttt tgtgcttggg tgtgttccac gacttcgttt
     7381 tcgtctttac ttaatttcac tttctggtac tgtttcattc gctcgagcat aaagtagatg
     7441 tgaatctcca cactagccag tccattcggc tgttctaaat ggttttcata aaaaacatct
     7501 tgtaatgtat gttggataac ttttaatgta tctttagcga agaattcttt caacgacgga
     7561 ctttgtggat cttctttctg catatttcgt aatgtcttaa caagcaactg tcttttttct
     7621 tgttcatcgc ctttaagaaa agttcctagt tttgttttgg atactagctg taaatggtga
     7681 tactcaaaaa agctttgcaa ctctgtcaat tctttacgaa tcgtacgttt tccaacatat
     7741 agtttttctg aaagttcttc aagactaaat ccgtcttttg ctactagcag atggaaaatc
     7801 aataactgac gaagctgttt tttatccatt tctgtttgag tctcttcatt aagcgctcgt
     7861 ttcagttgtt ccctttcgtg aggcagacca ttcaacaaga tacctgtacc tttttttctt
     7921 tcaaacatta aaggcagatg tttgtctctc aaccaagttt caatggtatt caaatcgcta
     7981 tgaatcgttc tttctgacac atggaaatgc tctgctagct tttttgttgt tatataatta
     8041 ttgttctcac ttaattgata gatcaaagat tgctgacgct gattcataat gtgcagaaac
     8101 tcccttctaa ataaaaaatc gatcatgata acacatctat tatgtctcat cacggtcgat
     8161 ttttcatctt atgttacatt tcgcttaata attggtgaac tgttgtttta tctggcgctt
     8221 ccatcagtgc ttgacggaag tcatcatcca tcaatttacg tgatagacgt tgcagtattt
     8281 tcaagtgcat atcaccttgg ctgttttcag gtacagcaat cataaagatc atatgtgcag
     8341 gttttccatc taaagaatgc caatctaccc cagattgact tctggcaaat gccactgttg
     8401 gtttaagaac ataagctgat tttccatgtg gaatagcaat ccccataccg attcccgttg
     8461 tactttcatg ttctctatca tagatgactt ctttaaatct ggttttatct gtgatcgccg
     8521 ctgttttttc taaacgttgg actaaatctt cgattactcc gtcttttgtt gttgctgttt
     8581 cacctaaaac gattgcttca tctgacataa tatccgtgat agaatcaaaa gtttcagttt
     8641 tcgtataaac atcacttgtt tcagtctctg atagatctgc tactgtcaca ccatttgctg
     8701 cagcaaccgc agtttgttgt aatgtcggtt taaggattct taacattagc acggttacag
     8761 caataccagc aatcactgct acaaaaaaca tggctacgtg atccactgct cctaaaaccg
     8821 ctacgatcgg accgccatga gctacatgat ctgtcactcc tgaaagcatc gcgatcacac
     8881 ttcctgtcat tgccccgatt acaatgcttg gaatcacccg tagtggatct gttgaagcaa
     8941 acggaatagc gccttctgta atcccaaaca gtcccattgc taaggacgcc ttaccagctt
     9001 ctcgttctgt tttcgtaaat ttggttttta aaatcaatga tgccagcccc aatgctaatg
     9061 gagggataca gatagctaca gcaattggcc ccataatgga ataattgcct tctgcaatca
     9121 agccagatcc gaacaaaaag gctactttat tgaacggccc ccccatatca aaagcaatca
     9181 tcgctcctaa gatcatagct agaacaattt cacttcctcc ttgcatccct gctaaaaagt
     9241 gagtcaatga actaaataga agagctactg gttctccaat aacatagata aacacaaaac
     9301 caactgccaa tgatcctaaa attgggataa caataattgg catgatcgat tgtaaagctt
     9361 tcggtactcg cactttcttg attcctaaag caatgaatcc tgctaaaaat ccagtgatga
     9421 tcccgcctaa gaatcccgca ttcgcatcac taccatataa tgctccgttt gccgctaaca
     9481 tcccaccaac aaatccagga acaagtcctg gacgatctgc aatactttgc gcaataaatg
     9541 cagatagaat tggaaccatc aaccccatag cgatacttcc gatactattc atcgttgccc
     9601 aaatcgttcc ttctggtatc ttgatccctt caggtgtagc tgttcctcct aatgttaaag
     9661 aaatcgcgat caataaccct cctgtaacta cgaaaggtac catgtacgaa acgccattca
     9721 tcaaactctt atagattttt ttacctaaat tttctgattc acttttattt tcggaagaaa
     9781 taattgcctc cgtactttta tagactggcg cttttcctgc taaaatatct ctcaccaact
     9841 catctgcatg gtggattcct tcttgcacac ctacggttat caaaggttta ccgccgaatc
     9901 gtgatttatc tattttagta tctgctgcga tgattacacc agctgcttct tcgatatctc
     9961 ttgcagtcaa ttcattttca ataccgatgg aaccatgtgt ttcgactttt acctcggcgc
    10021 caactgcttt ccccgctttc tctaagtttt ctgctgccat gtatgtatgg gcaataccaa
    10081 ctggacagga tgtgattgca ataatttttt tcattttcct accgcctttc tacatttata
    10141 gtatataaaa acggtttctt tttattaagc acaaaatgat tccgtaataa ctgaaattct
    10201 tggaataaat agaaatttcg ctaaaaaaat tgatatacga tttattatgt tgaataaaat
    10261 aatattggaa aatcttacat ttctgtactt ctgcttacgt acatctaaat tattatctac
    10321 attaacaatt tccgtaatta attataaaaa gcttattcca tttaatttca ttctggaata
    10381 agcttcttat tttctaatga gttagtga
//
LOCUS       DACEUF010000087        10154 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.091, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000087 DACEUF010000000
VERSION     DACEUF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.091"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(560..1600)
                     /locus_tag="IXG20_002219"
     CDS             complement(560..1600)
                     /locus_tag="IXG20_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1610398.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="IXG20_002220"
     CDS             complement(1949..2275)
                     /locus_tag="IXG20_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610399.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3050)
                     /locus_tag="IXG20_002221"
     CDS             complement(2262..3050)
                     /locus_tag="IXG20_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1610400.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3844..4023)
                     /locus_tag="IXG20_002222"
     CDS             complement(3844..4023)
                     /locus_tag="IXG20_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610401.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4020..4370)
                     /locus_tag="IXG20_002223"
     CDS             complement(4020..4370)
                     /locus_tag="IXG20_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610402.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4363..5688)
                     /locus_tag="IXG20_002224"
     CDS             complement(4363..5688)
                     /locus_tag="IXG20_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1610403.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6138..6752
                     /locus_tag="IXG20_002225"
     CDS             6138..6752
                     /locus_tag="IXG20_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1610404.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6917..7741)
                     /locus_tag="IXG20_002226"
     CDS             complement(6917..7741)
                     /locus_tag="IXG20_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610405.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(8379..8636)
                     /locus_tag="IXG20_002227"
     CDS             complement(8379..8636)
                     /locus_tag="IXG20_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1610406.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(8629..8898)
                     /locus_tag="IXG20_002228"
     CDS             complement(8629..8898)
                     /locus_tag="IXG20_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1610407.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(9029..9208)
                     /locus_tag="IXG20_002229"
     CDS             complement(9029..9208)
                     /locus_tag="IXG20_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610408.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(9589..>10154)
                     /locus_tag="IXG20_002230"
     CDS             complement(9589..>10154)
                     /locus_tag="IXG20_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610409.1"
                     /translation="EQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEK
                     YIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDD
                     YYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYT
                     YDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         3101 a         1854 c         1458 g         3741 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgtgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgttac taataattcc cttcttatag tgcattttct tagaaaaaaa
     3361 caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg attgatatta
     3421 tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa
     3481 agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata
     3541 aacgaatgaa gcaggtaatg attttattct acgttgtcgg gttatccagc ttgcaaccgt
     3601 tccttgtggg taggcatata actcacaaaa ttgttctaca gtgattccta aattcttgat
     3661 aatgtaaaca ttgatcgggt gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc
     3721 tactttcttt tattattgcg tttgcaataa tagtttaaca cacgcattat tctagtatct
     3781 actcctattt gtaaaaacaa aaaaggattc atagagataa ctctatagaa tccgtaaaat
     3841 accttatata tataacggga agatataaaa cctaaaatta tgataacctg ctgcagcttc
     3901 tacatagaaa gagaagcgct caacaccaac ctcatttatc aaacgttggc cataacccca
     3961 aatctcttct gatagctgat caaaggttat atactttagc tcatattgct ggataatgtt
     4021 cattctaatc gactccattt attatcctgg taaaattcga tatgacgtat ttcgtcgtag
     4081 tgaattttgt cttttccaac gtagatcccc agctcatcac tacctaagat cttgccaatg
     4141 atatcttctg gatagtatcc ttcagcgttt cgttcttctt tttgaataga tactgatcta
     4201 ttttttatga ctgcagcgtt caatacttcc gcaatctctt cagtcgtcat ttgttctttt
     4261 gctaaatttt gactggttcg ttgttcattg tcggcatcaa tttcttcagt atgatcagat
     4321 aaataaaacc ctgcccactt taaacgtcca cgatcattgt aatcaatcat ttttaattcc
     4381 cccatttccg cccgcatgac caccaacaag tgtgcttcta gtaatgcttc tagcgccttc
     4441 taacattgaa gaagcatgta caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat
     4501 aattttatct aaattttctt cgtcaatttg cttttgtgga tcactaaata aatccagctg
     4561 aagcgaatcg gtgtacacaa gcttgctata ggttattcca acatgcctta cttcttgtcc
     4621 ttcataatac ttatcaaaca agaaaagaca ataattctgc aattcctttg tattatctgt
     4681 gatagggatc ttcatctggt gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat
     4741 gtgtaaatgg acacaacctg ttttacagtt atgctgccta atgcgtatcg ctacttgttc
     4801 cgccatttct tttaccacca gctcgatttc atttcttcta gtataatctc gtggtaacac
     4861 ttgtgaattt ccatatgatt tttcctttgt cggttctggt gggattgcta tatctgttcg
     4921 atcgatccca ttagcatgga agtacagctg taagccaatc acacctagtc tattcttaat
     4981 cgtataagga ttccagttag caagatctct aatgctcata atccccatct gattgagtct
     5041 ttttttcatt cgagagccaa ttccccaaaa gtccgtcatt tcaggaatat tccatacttt
     5101 ttcaggaacg ttttcgtacg tccattccgc aacaaatcct tcattatgct ttgcttcgtt
     5161 gtctaaggct agtttagcca aaaggggatt atctccgaca cctactgtag caatcaatcc
     5221 tagctcttct ttaatattct cctggatcat ttgagccagc ttctttctcc gttgacttcg
     5281 tgttccttca gtcgtaaaaa gattcagtga tcgggtcact tttaagattg attcatcgat
     5341 cgagtaaatc agtagatctt catcagccac atatcttctg aaaatattat ttacctgcat
     5401 atttcgcttg atatataggt tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg
     5461 ttgtggtaag tcacgtggtc tactcacatt tgtaatacca taccgctttt ttgcttcagg
     5521 agaagaagct aatatcaatc ctgaaccagt attatcagct cgactcataa caacaagttc
     5581 tgttgttaat ggatctaaat ttctttctat acattcgaca cttgcgtaaa agcttttcat
     5641 atcgattaaa aagtaatcat ttactggctc ttttgaataa tccatcataa ataacactcc
     5701 attctttcac attatacaaa catatgttcg atattgtaaa gaagaaatag ctaaatacat
     5761 aaaaaaagaa gcataggtat gctatcaccc tgcttcttct tttgatttgt atgatcaatt
     5821 tattctatca ctttactaat taatttgaaa atctaataaa aactctcact ctaaaagagt
     5881 gagagactgc aaattagagt acggactttt ttggagttcc gttctttaat attaacaaat
     5941 gaataaaatc cttacaacat ttgagaataa gttatcagta gacacttaca aaaaattctc
     6001 atactatttc ctccatcatt agtttttgtc tatataaggt ttaccctaaa tagacactgg
     6061 tataacattg aaaaatatgg cttcctcgtg tatgattagg gtgtatttta aatagttgca
     6121 gaaagtaggt aaaagttttg agagttgcat acatacgtgt ctcatcgatc gatcaaaatg
     6181 aacaacgcca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc atcgagaaac
     6241 aatctggcgc aacgattact catcgacctg ttttccaaga agcattagat tacgtccgag
     6301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattat gacgagatca
     6361 tcgcttcagt caattacttg aagaagaaaa atgtgaagct gatcattact agtttaccga
     6421 tcatggctga agcaattggc aatcctctcc tggataaatt cattaaggat ctgatcatcc
     6481 aaattttggc catgatcgct gaacaagaac ggactgaatc gaaacgtcgc caggcacaag
     6541 gaattaagat cgcaaaagca aacggcgtct acaaagggcg gcccaaatta tacagtgcag
     6601 acgcaaaaga tccccaacgt cgccttgttt acaaaagcat cgttgaagat cttaaaaatg
     6661 gtgttgcgat tgctaagatt gctaaagact acaatgttac gagacaaacc gtgtatagaa
     6721 taaaaaaaga cagcatggtc aatgacaaat agttgatcat gctgctttta ttatcttctc
     6781 atctttaatt tcctacatat aattactatc tataaaatta agtcttactc agaacagata
     6841 aagcataaaa ttccaacaaa ccatttttta cttaactaaa taaagagtct gttaatataa
     6901 tcttcactta tttacgttaa tcccctactg tatttgttgg ttccttatct atattttcta
     6961 caaaatcctt attatcttgt gatttagctt cgtataattc tttgtatgtc gacacattgg
     7021 ttatactaat tcctatagct agttcttgag actctacttt tctctctagc tcctttgaca
     7081 aagtgatttg agatttattt ggttccgttg gagtcaattc tatagattct tcataaaaat
     7141 ctctatacat aaagctatca ttaattttat aaaaaaaatt aaatgttttt agcactgcca
     7201 tataacattc aaaacattct tctatagttg gtttacttcc ttgaggagaa tgaaccaatt
     7261 tattacgttt ttctatgaaa tttttaattt cctttcttat ttctttcgga aactcaatat
     7321 taaagtaccc tttaaacatg ctccctaata atttgtctac agggggagat tgtaaattct
     7381 caaatattag agttaaatca ggtttctctt gttgataaaa ttcctttacc cctatttcac
     7441 aggctgtaat tatcgaaata aacctatctc tagtatttga tatattttta cttcttttaa
     7501 ggagtataag agaaaagggg atactttcgt gtccatgcat gtaattaact aattgttgag
     7561 cttcagaagt tatatctcta attatatagt taacggaatt aggaagtgtc tttaactcta
     7621 atgaaatttc tatccaatta ctatcttcta atctgtatat gctcgaagta taatttgatt
     7681 cgtcagcaag catcgctaca tcccaaacac tgatcataaa atcttttaaa cgttcaatca
     7741 tttcagcaat attgctaaaa tcagctaatt tttctttaga ataaaaaaaa acatttatat
     7801 cagaatcaat aaattctatt tttttattac tatatcgtca tcattatctt gaccatttat
     7861 gtttattgtt ccctgagtgg ggagtacttc tttcaaattc aagttaaatt tatattccat
     7921 tacttctcct ccaatatata aaaattactt aactgtatcc aaatttaata tgtaaataaa
     7981 tcattaattt ttagctaaat tctatgagaa gtttattttt gttgtttttg ctaattaact
     8041 aaaactagta caaaaaattt aaaatagatt attaaaccta ttgcttattc tattcagtca
     8101 tatatataag cgtcatcctt tagtaactaa catcccacac tacaatagta ttataacata
     8161 tctattcaac ttcaaattgt tgaaatttaa gccatttata tttacttttc ctcaacttta
     8221 gaaatgattt tactaacctt atatccgtaa tacgcgtaac aaacacattc attaataaca
     8281 atcaacacta agctatatta gctttttaaa tgactagggt aaataaaata aggatcaaaa
     8341 acttttaagt ttctgaccct ttccttactt ccgattggtt aatagtgatc ttttgcagaa
     8401 taaataaata tcgtttcatt ttcaactcta tatatcagtc tatgttcatc tgtaattctt
     8461 ctggaccatt ttccagataa atcatgcttt aatggctcag gttttcctaa tccagcaaag
     8521 ggggaacgat cgatatcttt tattaacttg ttaatctttt ttatattgct tttgtttcct
     8581 tgctcatgcc aataaagata atcatcccaa gcatcatcag accaagcctt aatcatcaga
     8641 ttcaacctcg attaagtcat gtgttttaaa tgcacctttg gagaattgtt catctcctcg
     8701 acgaattttt tccatgacgt aattattaga aagtgttctc aacgtttctt gcatagaatc
     8761 ataatctctt tttgataata caacaactgt atcttctaca tctttacttg ttacaataag
     8821 tgtttcagca tcctcattaa cttgtttcat ataactacgt aaattttggc ggaaatttga
     8881 ataagctact gcttccattc ctttcacccc ttatttattt ctataaaaca attgtacatt
     8941 atattgtaca attaagcaaa tgattaatat tctaacttct tataattaaa agtgctatca
     9001 cttttagttt ttaggataaa aagtgacgtc actttttgag tgctggtacc tcaaatcctg
     9061 ctttttcttg ggcaatcaat aaatctaacg caatacacat aatcctggat tcacttttgc
     9121 cgtacttctg ctttagttgc tgcaattctt tttgtctctt ttcatccaac gtaacgggta
     9181 accgtttatt tcctattgca attggcatta gtactctctc ctttcatcga aataacccta
     9241 tgaaaaatat ggctgcaatc atcttgaata gtagactcac aagggctaat acaaagcgcc
     9301 aaaagatcaa acaaaatacc agtattgcca aaaatactac gaagtttaaa tgcacagctg
     9361 cagcatggcg cataagtatt tcgccaacaa ataggactaa caatagagga atgattttat
     9421 tcatatggat ctccttcttt ctcttaggtt atcgggtgtt gcccgcttat ttcgcaccac
     9481 ctaagcggcg ggggcggttg gagcgattgg tgcgaaataa gaaccattcc cttagttact
     9541 aggttttttg tccttatttc acacattctc acttttcggt ataaatgatt attgcctttt
     9601 tttcctttca aatcgttgat atggcattcg tttacggtca agttcatttt ggatttcttc
     9661 tgtatcgtcg taagtatagg tgtctaaaaa atcagttaaa gtcgtgtacc agtcgtcgcc
     9721 tgggttatct acttcttcta catattccct caaaaatgct tcgggtacag cgagttcata
     9781 atccattccc attaacacga tagattccgt tccacacgtc aaaaatctag taaatacaag
     9841 gtaataatcg tctatcgtcg catctttgct gtttagctct tccatttctt cttccattaa
     9901 ttccatgctc tcaaaaattg aataatattc tgattctgag taatagtgtt tcctgcaaat
     9961 cgtgtagctg acaaagtttt cgaccagctc actcctttct aggtaaacat tcatgatgta
    10021 tttttctgtt tctagtcgta gaagctccgt tccttttttt accgtttcag gacgacgttt
    10081 atacacgtct aaacgattat ggctgactgt gaccttcgtt tgactcgttt cactttccaa
    10141 atacaattgt tctt
//
LOCUS       DACEUF010000088        10005 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.092, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000088 DACEUF010000000
VERSION     DACEUF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.092"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            201..680
                     /locus_tag="IXG20_002231"
     CDS             201..680
                     /locus_tag="IXG20_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610410.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            713..2941
                     /locus_tag="IXG20_002232"
     CDS             713..2941
                     /locus_tag="IXG20_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1610411.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3070..4713
                     /locus_tag="IXG20_002233"
     CDS             3070..4713
                     /locus_tag="IXG20_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1610412.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4786..5331)
                     /locus_tag="IXG20_002234"
     CDS             complement(4786..5331)
                     /locus_tag="IXG20_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ1610413.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5354..5839)
                     /locus_tag="IXG20_002235"
     CDS             complement(5354..5839)
                     /locus_tag="IXG20_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1610414.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5829..6641)
                     /locus_tag="IXG20_002236"
     CDS             complement(5829..6641)
                     /locus_tag="IXG20_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ1610415.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6780..8312)
                     /locus_tag="IXG20_002237"
     CDS             complement(6780..8312)
                     /locus_tag="IXG20_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1610416.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8421..9452)
                     /locus_tag="IXG20_002238"
     CDS             complement(8421..9452)
                     /locus_tag="IXG20_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1610417.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3334 a         1845 c         1920 g         2906 t
ORIGIN      
        1 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
       61 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
      121 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
      181 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
      241 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatatggat gcaaaagggc
      301 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
      361 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
      421 tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct tatcgttatg
      481 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
      541 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
      601 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
      661 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
      721 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
      781 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
      841 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
      901 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
      961 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
     1021 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
     1081 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
     1141 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
     1201 cccttctgaa atcgttcgag cgattgaaaa aggctattta ttagagattc aagagccgac
     1261 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
     1321 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
     1381 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
     1441 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
     1501 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
     1561 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
     1621 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
     1681 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
     1741 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
     1801 tacaaaagaa agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
     1861 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
     1921 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
     1981 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
     2041 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
     2101 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
     2161 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
     2221 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
     2281 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
     2341 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
     2401 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
     2461 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
     2521 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
     2581 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
     2641 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
     2701 gcttaatatt agtgacggtc agccaaaagc gttgccggat tatacaggca aaaaagcaaa
     2761 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
     2821 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
     2881 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
     2941 ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
     3001 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
     3061 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
     3121 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
     3181 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
     3241 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
     3301 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
     3361 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
     3421 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
     3481 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
     3541 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
     3601 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
     3661 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
     3721 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
     3781 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
     3841 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
     3901 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacatgca
     3961 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
     4021 catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg
     4081 tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa
     4141 ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta
     4201 gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg
     4261 gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa
     4321 caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg
     4381 atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg
     4441 atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat
     4501 gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg
     4561 gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg
     4621 acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct
     4681 tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg
     4741 gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct
     4801 aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt
     4861 tttcaaacga ggtctgacag gtacttggac atgcttgata tgcataccga ttgatgtgtc
     4921 accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg
     4981 gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat
     5041 ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc
     5101 acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta acgtacggat
     5161 gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct
     5221 tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc
     5281 attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa
     5341 ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag
     5401 gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc
     5461 cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc
     5521 ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa
     5581 catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa
     5641 tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa
     5701 gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta
     5761 cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg
     5821 ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt
     5881 ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt
     5941 tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa
     6001 aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca
     6061 taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct
     6121 ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct
     6181 gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta
     6241 atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc
     6301 acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt
     6361 gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg
     6421 ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa
     6481 gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg
     6541 cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca
     6601 gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat
     6661 aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc
     6721 caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt
     6781 taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct
     6841 gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg
     6901 acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt
     6961 tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct
     7021 aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga
     7081 tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa
     7141 tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct
     7201 ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct
     7261 gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt
     7321 ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat
     7381 acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg
     7441 ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc
     7501 tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc
     7561 cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata
     7621 ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg cactggagag
     7681 atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc
     7741 gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca
     7801 gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa
     7861 gcaggttgtt ccattaaata aagaatagga atctgttttg attttgttcc atttgaaatt
     7921 tctctcactc gcttttttgt ctcatcatca aataaaatga aagggtcttc taaacgatcg
     7981 ataaatactt ccattgttgt tgttggtaga tgataagaag tcatgacagg cactgcacca
     8041 aggtaagaca cagaaacagc tagaaggtat gtgtcaaaag cggaactctt ataaataata
     8101 actttgtctc cggatttcac tccaagatga gccaattgat aagcccgttt caacacactt
     8161 tcgcagcatt ttttgtaagt tgtttcaagt cctaattctg ggaaagctga aagtggctca
     8221 tcaaagataa tcggggtctc ggaaaagtta gctgcagctt cttgaaaatt cgtaaataag
     8281 ttcaaagggg aatacgttat ggtctccatc attcacactc cattatcttt tgttaagtaa
     8341 tatacaaatt caagataatc gtaacataat cctttatata ataacatagt ttattgtgaa
     8401 aaaaagatca tacatttgtt ttatatggaa gaaacccgaa cgaatgccgc taattcgtga
     8461 taagaaaaat caaccatctt ttcccagata tcttgaaaag aatgggatga gaggtatcga
     8521 ttaccacata aataatttac cgcgtcagca tcatctaaat atagttctgc ataataacga
     8581 tatgtttttc tgcatagttc tggcgatacg tttttatata ctgtaatttt gctcaaaatt
     8641 tggcgaatag tccgaggaga aatttgctga tcatgtgagg aacgaaaaac aaaagaattt
     8701 tcgtccgtat tttgtgctgt taacttaaaa tagttctcca gaaatggcat cgcttctttt
     8761 ggtattggta ctttattcag tctgttttct tttctattca gattaagaat ctgcttggtc
     8821 aaagatacat ctttacgttt aagttctact acttcatgga tctttaatcc gcaatacaga
     8881 agaatggcag taattgcaag gttcctcggt ctattctttt cttcaaagct agcttctctg
     8941 cttgtttgaa agccttcaat cgggataaac aaatgctcaa aatctttgaa aagttctgca
     9001 tcgatatatc gcggagctgc tttggttgtt tccggtaaac ttgtacccaa aagagaaatc
     9061 tcttcttcaa tcagtccttt ttgatacaaa aatcgtaaaa aatgatttaa tgaatattgc
     9121 ttccttgaaa tcccgtaata gctattttca aacaattgct ccgtttcttg gttcgctgta
     9181 gtttggaacg aacgagtatg tcgatagatt ttagctaaga aattctgata atcttgtttt
     9241 tctaaatgga taatcagctg atcatccact tcttcagaaa atacttttct caaataatca
     9301 gtaaacagat gaaaatcttt tgcgtattca tagcgactgc ctaatgaaag ggaagaacga
     9361 ttttcttcta aaaactgttg aaagacggaa gggagcaagt gtgtacgttg ctttaacttt
     9421 tcttgcagtt gttttctagt tttgctgtcc attacaaatt ccctccaata gtgttcattt
     9481 tctatatttt accgcatttc atcaaatgaa gatagacagt ttgtcttgat cttaggaagc
     9541 aaatttttaa ctaagtacta gctaaaagat atgaagtaag aagatacatt tttagttatt
     9601 ttattagtgc agtcattctt ttgatctttt aattgattta tcctcttttt agctatgtgg
     9661 ttacttcatg agcaatgaaa tggatttggt tcgttctaat ggcaggaggc tctttctttt
     9721 atttccgaaa aattagaaaa cattttttca gaatcgattg gctctccatt tattcaataa
     9781 tggtttgtct aacgatattg ttgggataat tcttaaagaa gtattagaaa tagttggtac
     9841 ttcattactt tacatgaaga tttattctat ggtaggagca attatattga gaattaatcc
     9901 ttttttatat aagttgtttg cttattgatg gattggataa attaaaccat cacaacgagt
     9961 ggaaattata tgaaattttt cataaaaatt catatagaaa caaat
//
LOCUS       DACEUF010000089         9873 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.093, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000089 DACEUF010000000
VERSION     DACEUF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.093"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(21..266)
                     /locus_tag="IXG20_002239"
     CDS             complement(21..266)
                     /locus_tag="IXG20_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610418.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(381..959)
                     /locus_tag="IXG20_002240"
     CDS             complement(381..959)
                     /locus_tag="IXG20_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1610419.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1036..1566)
                     /locus_tag="IXG20_002241"
     CDS             complement(1036..1566)
                     /locus_tag="IXG20_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ1610420.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="IXG20_002242"
     CDS             complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="IXG20_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ1610421.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2423..2818)
                     /locus_tag="IXG20_002243"
     CDS             complement(2423..2818)
                     /locus_tag="IXG20_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1610422.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2815..4227)
                     /locus_tag="IXG20_002244"
     CDS             complement(2815..4227)
                     /locus_tag="IXG20_002244"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ1610423.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="IXG20_002245"
     CDS             complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="IXG20_002245"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ1610424.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(5082..6542)
                     /locus_tag="IXG20_002246"
     CDS             complement(5082..6542)
                     /locus_tag="IXG20_002246"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ1610425.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6712..7869)
                     /locus_tag="IXG20_002247"
     CDS             complement(6712..7869)
                     /locus_tag="IXG20_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ1610426.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7903..9273)
                     /gene="radA"
                     /locus_tag="IXG20_002248"
     CDS             complement(7903..9273)
                     /gene="radA"
                     /locus_tag="IXG20_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ1610427.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9336..9863)
                     /locus_tag="IXG20_002249"
     CDS             complement(9336..9863)
                     /locus_tag="IXG20_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ1610428.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2508 a         2214 c         1784 g         3367 t
ORIGIN      
        1 atctcaggct cgtttgcttt ttaaactgct tcagttaaaa attcgatctc aactgtccga
       61 gtaagtacat ctgaatagct gtaagatact cgttcaaaag aattttcttc aggatccaaa
      121 tcaacaacga atactgaagg ataggtttct gttaaaacgc cgcggcgttc tgtttgacgc
      181 tttcttcctg tctgagcaac caacatgatt gggctgccga tacgaccttc taaatctttc
      241 ttgattgaag ctaaagttgt tggcatacag ttcacctcta ctatcaacta taccacatat
      301 ttttaaaatt ttcaagttta ccataaaaaa aaaagaaatg caataaaaat aaataagaag
      361 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
      421 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
      481 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa
      541 aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt
      601 tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt
      661 gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag
      721 atttgattgc ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg
      781 taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca
      841 taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac
      901 agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata
      961 aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact
     1021 gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt
     1081 ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt
     1141 attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga
     1201 agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac
     1261 tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc
     1321 cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat
     1381 gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata
     1441 atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag
     1501 ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt
     1561 tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc
     1621 aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat
     1681 ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga
     1741 tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa
     1801 tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac
     1861 atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg
     1921 ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa
     1981 attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt
     2041 tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac
     2101 tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac
     2161 tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc
     2221 ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg
     2281 cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg
     2341 tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga
     2401 ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc
     2461 ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt
     2521 cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt
     2581 tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca
     2641 gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat
     2701 gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt
     2761 aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc
     2821 ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa
     2881 caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc
     2941 ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa
     3001 gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt
     3061 cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt
     3121 tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa
     3181 agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa
     3241 attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc
     3301 actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg
     3361 atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt
     3421 ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt
     3481 ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg
     3541 cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc
     3601 aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt
     3661 tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt
     3721 acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt
     3781 ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc
     3841 aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag
     3901 tgtagcaggc tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc
     3961 agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac
     4021 atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat
     4081 tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg
     4141 tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct
     4201 ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga
     4261 acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg
     4321 gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc
     4381 gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg
     4441 ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca
     4501 atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg
     4561 atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc
     4621 cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat
     4681 gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc
     4741 gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc
     4801 atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc
     4861 cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac gcctgttata
     4921 cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag
     4981 accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct
     5041 cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt
     5101 aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca
     5161 tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca
     5221 gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt
     5281 tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact
     5341 tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg
     5401 atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg
     5461 attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac
     5521 atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt
     5581 tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc
     5641 aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata
     5701 aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag
     5761 attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata
     5821 tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta
     5881 ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc
     5941 gcaacagcaa aattataagt tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg
     6001 atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta
     6061 ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt
     6121 tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt
     6181 tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg
     6241 ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa
     6301 gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg
     6361 ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata
     6421 cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt
     6481 gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc
     6541 attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg
     6601 gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa
     6661 atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt tttattttga
     6721 ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg
     6781 tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat
     6841 acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta
     6901 ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc
     6961 agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt
     7021 ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag
     7081 cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta
     7141 catttcgaca gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg
     7201 accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac
     7261 aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg
     7321 accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt
     7381 tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg
     7441 tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata
     7501 gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt
     7561 gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc
     7621 gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc
     7681 tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct
     7741 gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga
     7801 gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg
     7861 tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt
     7921 caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt
     7981 acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac
     8041 tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa
     8101 acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc
     8161 taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg
     8221 attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt
     8281 gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc
     8341 ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt
     8401 tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc
     8461 atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc
     8521 tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat
     8581 atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa
     8641 aatggctatc ccatttgttt tagcgatttt gagtagttct gcagtggttt ctcttacttg
     8701 gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat
     8761 gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc
     8821 agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg
     8881 ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg
     8941 agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat
     9001 agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat
     9061 ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt
     9121 caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt
     9181 ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg
     9241 gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat
     9301 gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg
     9361 ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct
     9421 tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg
     9481 aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact
     9541 ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa
     9601 aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt
     9661 ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc
     9721 cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca
     9781 gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata
     9841 acttcaaatc ctctttttct caccggtttt cct
//
LOCUS       DACEUF010000090         9820 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.094, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000090 DACEUF010000000
VERSION     DACEUF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.094"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            55..507
                     /locus_tag="IXG20_002250"
     CDS             55..507
                     /locus_tag="IXG20_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610429.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            521..1210
                     /locus_tag="IXG20_002251"
     CDS             521..1210
                     /locus_tag="IXG20_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610430.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1335..3686
                     /locus_tag="IXG20_002252"
     CDS             1335..3686
                     /locus_tag="IXG20_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ1610431.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3766..4161
                     /locus_tag="IXG20_002253"
     CDS             3766..4161
                     /locus_tag="IXG20_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610432.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4486..5103
                     /locus_tag="IXG20_002254"
     CDS             4486..5103
                     /locus_tag="IXG20_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1610433.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5144..5443
                     /locus_tag="IXG20_002255"
     CDS             5144..5443
                     /locus_tag="IXG20_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610434.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5477..5644
                     /locus_tag="IXG20_002256"
     CDS             5477..5644
                     /locus_tag="IXG20_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1610435.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5689..6294)
                     /locus_tag="IXG20_002257"
     CDS             complement(5689..6294)
                     /locus_tag="IXG20_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1610436.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            6419..9406
                     /locus_tag="IXG20_002258"
     CDS             6419..9406
                     /locus_tag="IXG20_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAQ1610437.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            9555..9764
                     /locus_tag="IXG20_002259"
                     /pseudo
     CDS             9555..9764
                     /locus_tag="IXG20_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3359 a         1479 c         1879 g         3103 t
ORIGIN      
        1 ttatcttatc gtagatatat attataaatt taataagaaa gcgaggtgag aatcgtgaaa
       61 aaaacgatac taagtatttt ttcagtattt acaatattaa ttgggggcta ttttcttttg
      121 aatttagatt ctgttaattc aaacgctcaa ataaaagatc aaagagtagc tgaacataat
      181 aataaaaaaa tgagtttgac caaaaatatc tattctatgt cagatgaaga attgtataaa
      241 gaatttggtg atttatccgg ggaacagtta gaagaaagta ttccaggaat tagtgactca
      301 gataaaattg ttgtcttgtc tgctggtggg caattaatgg gagatggcaa atccgaatta
      361 gttagttata aacaaaatca cagacagttt attgatgtta atactgattc caacgctatt
      421 tcgattaagg atctaaaaga gcttttaaat agtaaaagaa atgaattaaa gcagataaaa
      481 gaaactaatt cagtggaatt agagtaaagg aagattcatt atgaaaaaga tttttggtgt
      541 ttctgttggg ctacttatga tgttaacatt tggaaatgca gtatatgctg ctgagaaaga
      601 agttatttca gatccagagc agtacgcctc acgatacttt gctgattttg aagaagaaga
      661 taaagtactt gcattgccag aaggtggttt tttaaaaggg gaagctaaaa ttgtatctat
      721 ggataatcca gatataattc aaggttcata taattcagaa actgatcctg cttcagtttc
      781 agtgacagaa gcaaaaaaaa ttgttgctga aaaattcaaa aatgatattg aaactactcc
      841 aattatgcct cgaggagcat ttccacccga tgttggtact agcggacacc ttcttgtact
      901 agcaggagga ggaagctata aatcacagcc atttagtggt gcgggatggc gttttggagg
      961 tatgggtgta aaaccagctg atggtacagg caacttttta cgttggaaat cctacattga
     1021 cgatggaaga gtaggaaatg taaatcaagc caacgcaact aaatctggtt ctattcaagg
     1081 aatggcattg tatgctcgtg aaggattcaa atactttgca ccaaacgggc ctggttggac
     1141 aatgaatata ggtatttatt acacttataa tcctgttgca ggtacgtatt atgttgttca
     1201 aaatatttaa taaaacagca caaagagatt tctttgtgct gttttattat ttttagttgt
     1261 tgacaaacac taaaatataa aatataatca acacataaac gaacgtatgt tcggcaagaa
     1321 agcaggggat ttttatggat gagatggttt taagtactca gaaatggcta aacaagaaat
     1381 acagtaatgt tacagggttt gataaggttc cagaaaatgg gcgaacaggg tggcctacaa
     1441 tctatggact tattgaagga ctacaagtag aacttggtat tactaatcta gtagctaatt
     1501 ttggacctac cactgaaaaa atgtacgata atcaggttac tcctaaatgg ggcaaaaatc
     1561 tacctaaaaa tattgtattt cttattcaag gtgccttttg gtgtaagggg attaatccag
     1621 gtggcttcga tggggtatat actccatatt tagatactgc tgtaaaagag ttgcagactg
     1681 atgcagggtt taatggaagc gctgtaactg gagttttgga tgcgaaatgg gccaaagcgt
     1741 tatttgatat gagtgctttt gttcttgttc ccggcggaga tagtaaaatt agaaaaatgc
     1801 agcaatggtt aaatgtaaat tatttagaat acacgggaat aatgccttgt gatggtattt
     1861 atcaaagaaa ttcaaatcaa gcattaattt ttgcattaca agctgaacta ggatattctc
     1921 ccagtgaagc aacagggtct tatggtaatg ggacaactag taaaacttat cctgtgtctg
     1981 aaggtaactc gggaaactat gtaagaataa ttcaatatgg attatatgtt aatggttatt
     2041 ttgaagacgg tacattcgat ggtattttca ctaaatatat gggtttagaa gtactggcat
     2101 tccgaaaatt tatggtattg cctgaatata ctgaaatagc agatgttgtt gtaataaaag
     2161 gactattgtc tagcgctgga gatattagaa gatctgcaga tggcgcagat acttctacgc
     2221 aattaactag atctcaaatc caaacattgg ttgataatga tataaaaata gttggtagat
     2281 atcttactgg tactgttgga attggaaaag atgaacgaaa taagtatcta actacagagg
     2341 aattgaataa tattttttct aaaaatttat ctgtgtttcc tatctatcaa gatggaggtg
     2401 cagcattagc ttattttact tatgatcgtg gcttatcaga tggtaagaag gctatagatg
     2461 cagcaaaaaa tctaaatata ccattaacaa cagttattta ttttgctgtt gatctagaca
     2521 tgttagggga agaaattctt gcatatgctg gagaatattt taagggggta tctgctgtaa
     2581 tgtcatatag cgggtatcaa acgggtgttt acggaacaag aaatgtttgt tctcaaataa
     2641 tcaataacgg gtatgcttcg tttgcatttg tttcagatat gtcgactggt tatagtggga
     2701 atttaggttt tcctatgcct agacaatggt cttttgatca atttgttgaa tttaccatag
     2761 gaagtggcaa cggagctgtt ggtatcgatt taatcgctac atcgggtaga gatagcggat
     2821 ttaatgagtt atcaaatgat actaataatg ataattacat tgccaaatac aatcagaagg
     2881 ttataaatca gatggtacgt gcgtatcaat atctatcaca agctggtctt gataatcctt
     2941 ggaaccctca tttgacattt tatagatatg taaattattc tggtttgtca tgggacatca
     3001 tttcttctcc tgtaacagag catgatagaa aaatttatga tgaatatagg aataaattaa
     3061 ctaatagcga gggcctgtat aattatttta tagatcctaa tactggttca ataataggtt
     3121 tgcctcactt aattgtgacg ttacaatcac agatgtttct agccgacaca atcaatgata
     3181 gtgtttctga ctttgcaggt tggttaggag atttaatgac ttgctggggt gaagttaaga
     3241 aattaggaat acaaatggag caaggtgtat ttgcattagt aggtacggct gcaggtggaa
     3301 catttagtct agaagattta taccaagatg ttgacgcggt aaatgtttat accttagcaa
     3361 aaagaaattc tgattcaaca gctttatcgg ctttaaaaac gtactattgt gataagggtg
     3421 ctattgatag atttaatcaa tttgttaaga acagatttgg ttcttcagat aaaatcgctt
     3481 cagtaacaaa tagtttatta gaaaatgacg aaacagttga tatcttaggt gtagggataa
     3541 tttcattttt cattgataaa atagttaata attcacttgg gacagatttt acttatcttg
     3601 attttattgg agatgagggg aaagttgcaa atggattttc tgaaaaaatt attcaatttc
     3661 aagaaaatag aagtaaactt aattaagaat cgtttactat ttaaacagtg tccttttcct
     3721 ttttaaatat atacgataaa ataagtatat gaattgtaaa aggacatgat gattatggaa
     3781 gaaatagaat ggaataatct gactcaaaat aataaaaata attctggatg tggaattatc
     3841 tttatgtttg ttatagtttt gggaatagta gtacttttaa taactgtcaa gatagtagat
     3901 tactttgcga atactcctga agaacaaata cagagaaaac ttcctgagtt tgaaagagta
     3961 ccaccttatg atccaagaga taattcattt caagtaaaaa aattaaaaga gtctccaaat
     4021 gaaatgtggt ttgaagttac agttgaatca agcgacttca aagaaaaaaa gaaagaatac
     4081 aattatattg taaaggcaaa agtatcaaaa agatttattt ttaaccctaa attagaaata
     4141 tatagtgttg taatagaata atagtgttaa aaaaattgta aaagttaaag aagtcaaatt
     4201 ctgatgtgaa aaataaaata taccatggaa gacataaaag aaaaactgga taactagttt
     4261 atttaatggc agaccgttaa ggcctgccat ttttatgtat tttagcggaa taaatataat
     4321 catgcgggag tgtaaactta ctgtttcaaa aagtatactt tttgacatgc cttgattttc
     4381 tttgtttatg tatggcataa taaccacata atacaatttg aaacgtttct gatttaatct
     4441 gattgttttg aaacaatttt agtgaaaaaa gggtagataa aaacgatgag aaaatttttt
     4501 tatatccggg tcagtagtta tgatcaatcg atcgaacgac aactgatttc cgcgaaggaa
     4561 ttaggaatcc ctgaagaata tatatttgta gaaaaagcca gcggcaaaga ttttaaacgc
     4621 cctgaatatc aattaatgaa acggatgttt cgaaaaggag atttactcta tctacaatca
     4681 ttagatagac taggcagaaa taaacaaatg attttagaag aatggcgaga attaactaag
     4741 gaaaagcaga ttgatatttc tgtgttagac atgcctctgt tagatactgc tagttacaag
     4801 caactagatg ggttagagac gcttatctct gacttaattc tgcaattact cagctatatg
     4861 gccgaagacg agcggaaacg aattaatagc cggcaaagag aaggcgtaga agctgcgaaa
     4921 caaaagggtg taaaattcgg cagaaagaaa attgctttac ctcctgaatt tcctgcgatc
     4981 tattctgact ggaaagcagg aaagataact gcagtctatg ctatggatca attgggcatg
     5041 aaacgaaata ccttttatcg acgggtgaaa gaatatgaac ataccttgca ccacttttct
     5101 tgatagatat aacagctatt agacgggaag ggagagtttg aaaatggact taacgaatca
     5161 aagggtcgtt tttactggaa cgctgcagca aatgacacga acccaagcca tcggtttggt
     5221 tcactcttta aacggccatt gtcaatcaag tgttacttca aaaacaaatt tcctggtttg
     5281 tggtcagtat tctaagtcgt tgtttgatga ttctctctat acaaaaaagg aacaaaccgc
     5341 gcgggacttg attgcgttag gtcatgagat taccatttta tcagaagtcg aattttatgc
     5401 gttaattagt caacagttga aagaatggca acggtattta taatcgttag taaaatttac
     5461 aaggaggaaa atttttatga agcgaaaatc ttatgatcgt tggggcattg aaataactag
     5521 ttttaatcga aatattgctt ttcacgtgga gtgtgactgt ggggaattag caaaaagtgt
     5581 ttatcaaaaa aattactttt actgtcctaa atgccatcgc cgatacgggg tacatggaac
     5641 atagaaacga aatagtgcgg tagaaaagcg aatgaatgta gtatatttct acaaatcatt
     5701 cgctttcttt attttataaa tagttggtcg agagatcccg ttttcttttg caatctctgc
     5761 aactggaatg ccttgcttta acatgtccac cacggtttta aaaactaagc gtttttgcgg
     5821 atctgttgag ttaatagaat actcgattgg tcgtccctta tatcggccgg ctttttgagc
     5881 caataatact ccttgccgct gacgttcacg aattttctca cgctcgtttt gtgcaatata
     5941 acttagcagt gataaaaaca tatcgaacat agcggtatcc aggagttcat ttccagtatt
     6001 aaaatctaaa aactgtgcat ctaatatttt taacgctact tttttctgtt tcataaaagc
     6061 aactgtattt tttatatctt cataatctct tcctaaacga tctagtgatg taacgatgac
     6121 gcaatcacct tcacggatat aatttagtaa tttttgaaac tcaggacgct cagtatcttt
     6181 accactggct ttttcttgga ataatttatc aatgttaagt ccttccatca ttgccaattg
     6241 tcgatccaaa ttttgatctt gtgaagatac tcgaatatag ccaatttttc ccataaaaaa
     6301 ctccttttta aaagtgatat aatttacatg taaaatttac ttgtagtttt actgctatca
     6361 tagcattttt aaaaataaat tttatgtaga cttaaaattt acttcatgga gggtttaaat
     6421 ggatgaaatc aaaagtaaca aaaaagcatt tggcatttat ttaggagaac ctagtacaga
     6481 acaattaaac caattttttt atttgcatga aaaagattta gaattgatgg agactatacg
     6541 acgaccagct actagattag gttttgcact acagttagtc tcactacgtt ttttaggaac
     6601 ttttatatta gatcttacca atattcccca aaatgtattg agttatgtag caactcaact
     6661 aaatattgat cctggagaaa ttatctacta tagcaggaga cagaccaggt atcagcacac
     6721 aaaattgata aaatcacatt atggatatca agatttcaac caaccggaaa tagaaacata
     6781 cctagctaac tggcttttaa atagagcgct gtatacaaca gaatcttcag atatgttgtt
     6841 tgatatgctt ctaaaaaaat gtctggatga aaagattgtt ttacctggaa ttactacttt
     6901 ttctcgtttt gttgctagtg caattgaaaa agctgaagag catttatacc gacaattgtc
     6961 tttggttcca actgaagatg agagaaagcg gcttctcaat catttagaat taattggaac
     7021 tccagtttat ggggcaataa tcaaaatgga cattttgaga accccgttag ctgacgatag
     7081 tcataaagaa atagccagag gttttgaccg actaaagcaa tttcaagctt tctctactga
     7141 aaaatggaag atggaatcta ttcctgaagg gaaaataaaa gttcttgcaa attatgcatt
     7201 taaagctaaa gctcaattaa ttcaacgaat gagtgaacag aaaaaactgg cgttgttagt
     7261 ggcatttatt ttcatataca aacgtaaggc tatggatgaa caaattttag cactatctaa
     7321 tttttttgaa actgttttta gacgcgccaa aaataaggaa tctaaagaac gtcttcgtac
     7381 cataaaagat ctagatcgag ccgcatcgac actttcagat attgtagatt tagtaatgtc
     7441 cagtcattct gatactaatg aactatccct tcgcgatcgt atcttagaaa aatattctga
     7501 ggaaaatgtt accgaagctg tatcgcaagt tcggctattg gttaaaaatg atcgtgaacc
     7561 aatagccatt tctgaacttc tgaacactta tcgaaagttc agaaaattta ttccggatat
     7621 tttaactaca ctaacatttg acagcaatga ctacggtaac aactgtaagg aactctggac
     7681 cttcattaat aatagatttc ctaagccgat tacttacaaa atgtttggag ctattaaaca
     7741 tactcttcca aagaaatgga tttattacat tcatgaatac cctgaacaaa ctaataaatg
     7801 tgtattgatt gcagctgtcg aattgttaat tcaatcttta aaacgccatg atatttatat
     7861 tcctcagagt gtcttatata acaatcctat ggattgcttg attgatgaga aaacttgggt
     7921 tcaacaaaaa tacgttttat tagaacaact agatcttcct tcttcagcaa atgaaatgtc
     7981 tttatggtta aagaacgatt tggaactgtc ttataaagaa gcgctaaatc gttggcctac
     8041 ttctaaaatg gccagaatag aaaatgcaga tcgcctggtt gtctctagac ttaaaaagac
     8101 atatgaaaaa aaggaagata agtcttttag acgtcgcatt cagcagttac ttcctaaaat
     8161 tgatttgtca gatttgttat tggaagtaaa ccaacagtta aatcttactc aatcatttaa
     8221 ccacttaagt gacaaggaca ccaaaatgaa agacttggat attagtttat tagcagttct
     8281 tttatcagag tcctgcaata ttggtttttc cccggtttca aaagaaggat ttgattcttt
     8341 aaagtatgat cgtcttgtct atgtcaatca tcattatatt cgagtggata cactttcagg
     8401 agctaatcaa aaaataatac atgcacacag gaaacttgcc acctctaaga tatggggtga
     8461 tgggcatatg gcttcagctg acggtattcg ttatatcaca ccacaacgtt ccctatattc
     8521 tagaagtaat ccaaaatatt ttggccgtgg tcgtgggatc acattctaca attttgtctc
     8581 tgatcaatat atagggttcc acggaatggt cgtttcaggg acactgcgag attcgcttta
     8641 ccttctagaa ggattcctca atcaacccag cgaattaact cctaatcaga tcatgactga
     8701 tacagctggt tatagcgata tgatttttgg tctctttggc ttattagggt ttcaatttag
     8761 tccaagaatt gccaatgata aaggaaccaa actttggcga atcgacgcta acgctcacta
     8821 cggggaactt gatacgttgt ctcaaaattc aattaatatg gaactgattt atacccattg
     8881 ggaggacatt cttagggttg caggttcgtt gaaatctgga aaagtgaatg caacagaatt
     8941 aacgaaatct cttcaacgga atgggcaacc cacgccttta ggaaaagcaa taactgagta
     9001 tggaaaagtt tataagacga aacatcagct tcgttatatt tctgacgaaa tatatgcacg
     9061 gcaaatttta gaacaattga ataaggggga agctcggcat gccttatgcc gtaatatttt
     9121 ttatggacga aaaggaaaac tctaccagac ttattatgat gggatggaag agcagctcaa
     9181 tgcattagga ttagttacta atgccatcat atattggaac tcactgtatt tagaaaaagt
     9241 atttgaacaa atgaaagctg aaggattcga ctgttccgaa gaaatgatac gcaggcaatc
     9301 tccactaatt agtgatcaca tcaattttgt tggtaagtat acgtttagat acgaagctga
     9361 attatccaac ggtaagttac gtccattgaa tgtagaagaa ttctagcaat gttcgggttt
     9421 tgcatgctac cgcactattt cgtatgactg ttccagcgaa tcacttcgaa tagaaaaaga
     9481 gaaaaacacg agaacagcgt taaatcaacg tttcttgtgt ttttactttt gtgctaccgc
     9541 actatttcgt ttctatgttc catgtacccc tttatcaaga agcgcaaaca cgccaaattg
     9601 ccgattggtt gatcggcatg aacgcaagcc ctttatatac cttgaattta caacaaaaag
     9661 gcgtacaagg tacattttca ttaggacgtg tacaaacacc gactttatat ctcatttatc
     9721 aacgccagga agcaatagaa aactttaaaa aagaaccttt tttcctgaat aattcatagt
     9781 tttactaata gtctttccta acattgattt aatagtcttt
//
LOCUS       DACEUF010000091         9470 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.095, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000091 DACEUF010000000
VERSION     DACEUF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.095"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            236..1042
                     /locus_tag="IXG20_002260"
     CDS             236..1042
                     /locus_tag="IXG20_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1610438.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1054..2274
                     /locus_tag="IXG20_002261"
     CDS             1054..2274
                     /locus_tag="IXG20_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1610439.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2264..3850
                     /locus_tag="IXG20_002262"
     CDS             2264..3850
                     /locus_tag="IXG20_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1610440.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3889..6027
                     /locus_tag="IXG20_002263"
     CDS             3889..6027
                     /locus_tag="IXG20_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1610441.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6315..7706
                     /locus_tag="IXG20_002264"
     CDS             6315..7706
                     /locus_tag="IXG20_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1610442.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7765..8574
                     /locus_tag="IXG20_002265"
     CDS             7765..8574
                     /locus_tag="IXG20_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1610443.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8546..>9470
                     /locus_tag="IXG20_002266"
     CDS             8546..>9470
                     /locus_tag="IXG20_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1610444.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLA"
BASE COUNT         3278 a         1448 c         1905 g         2839 t
ORIGIN      
        1 aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca caaaatatta
       61 tagaacccct tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta
      121 gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa
      181 atatggtaaa atggcacttg actgtaattc actgaaatcg cttggaggaa cacaaatgtt
      241 taaagatttg tatttattta tgaaagacat ttatcagaat agaaaattac tgctacagtt
      301 ttcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct tatgggcgtt
      361 tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg gactgaaagc
      421 acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga tgattccctg
      481 gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat atacttattt
      541 agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc tatccaatct
      601 gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa atggtatata
      661 tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg ctttcttgac
      721 aggattgact tggctgacgg catcgatcca accattttta cctgatatca tgcaattaat
      781 tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc ctaatcagtt
      841 tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg ttcaaggtta
      901 tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata ctttgtattt
      961 ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta gattgaaacc
     1021 acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat tagactaaaa
     1081 gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg tgaagccctc
     1141 aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt gaatgtagaa
     1201 ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa atctacaatg
     1261 ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat cgaaggaaaa
     1321 atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg ctatgaaaat
     1381 atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga aatggtcgat
     1441 gatatcattg aatttgctga tattggcgac catatttatc aacctgtaaa aacctattca
     1501 agtgggatgt tcgttcgtct agcttttgct gtagcgatca atgtgaatcc tgatattttg
     1561 attgtggatg aagcattggc agtaggagat cttgaattcc aactgaaatg tatggaaaaa
     1621 tttacagaaa ttaagaactc gggaaaaacg attttattcg tttctcatga tattagttcg
     1681 attcgccgtt tttgtgatcg ttcctattgg ttgaaaaatg gagaagttgt cgaatatggt
     1741 gatacgatgg aagtaacgac gaattacgaa aactttttga agaaaaaatc agttaaaaca
     1801 gtagatcgaa ataaaaacca agtagaacgc ttcgcagctc cagatatcgt agacgtgacg
     1861 aaagccgaat tgttggataa aaataaaaat ccagtggata tgctggaaca aggagaaaaa
     1921 ctatacgtta aagtaaccta tgaagtaaaa gacgatacaa tcaaaaatcc agttttagga
     1981 attgctctgc gtacagtcga caatttttat gtgtgcggac tcaatactct actagatggt
     2041 atcaagatcc cttggaaaaa aggtgagaat atcttttatc tggagtatga aaagttaggt
     2101 ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc aacggttcct
     2161 ttggtataca agacaaaata catgaacttg tttgtcagcg gttcttatat cggagaaggc
     2221 atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat atgattttga
     2281 aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca aaccaggaag
     2341 taaagtttta gagtttggtc ccggaaacgg tcgattaacg aaacatttga tcggagcaaa
     2401 acaatgtgaa gtgagcattg ttgagctaga taaggaatta ttcgattttg tcagcgaatt
     2461 tgcccaagat ggattttatg gagatatcga aagttttgaa tgggcaaact attatgctgg
     2521 acagacattt gattacgtct tgtttgcgga tgttttagaa catttagtag atcctggaaa
     2581 aacattgaag aaagtcagag aatttttgaa tgaagaagga gagatcctga ttacattccc
     2641 taacttagca cataattctg tcatgatcga tttgttcaat aatcaattgc cttgggcttc
     2701 ttatggtctc ctagatgaaa cccataattc tttttatact catgatggtt tccaaaaagt
     2761 ttttgaaaaa gccggattat tcattaatat tgaagattac ttgtacttgg ctgtaggaga
     2821 tacagaatta aaatcaactt atgaagagtt gccggaagct gtacgctatg atttcaaaat
     2881 gcgaccattt ggtgaagtct accaatactt tttctcattg atgaaacatc cagtagtaca
     2941 aacttccatt gcacagccac aaaactctaa ttatgtaaaa gtactagaag taacccagca
     3001 aacgaaacaa gatgaaacaa tccaacaaat accattcaat aatttcacag gggaaaatga
     3061 gacactaagt ttccccgtgt cagaagcaac agaaagaatg gtgtttcgct tcgcacagca
     3121 acctagcttt atcgaattca gtgcagaagc tgctggagaa aaactcatat ttatcgattc
     3181 aaatgctgta gtcaaaacag taaatgattg ttatttgttt gatgggaaag aaataccaga
     3241 gttcgctcta acggatattt ctgggaaaga agtgactatc cattgtcact atcgctttat
     3301 cggggaattg acaccaacaa tgaaagaatt gctggaaaca attcgtccta tggctgaagt
     3361 cacaaagcag ctttctgaga aaaatgatga attagaaaga gaaaatcagc atcttcttga
     3421 ggaaaataca agactagacc atacgttaaa aacaaccaca aatcgctatt gcacattgtt
     3481 ggaaagtgat gaatggacga tcaaacatag actaggacga cgtaagaaaa aaacatcgaa
     3541 aaaaatacaa gaaaaagagc tttctatatg tattgatgag aaaatatggg atgcagaaac
     3601 aaaaatatta aaaatcatcg gatggggaat cgctaattcg gatcgccagc cattgtccta
     3661 taagttatca gctgaccaat cgccattttt cgaagcaatt cctgtttcac gagatgaagt
     3721 caatcaagca gaacagctcg caaaaggaac agaagcggga tttgaattac gaatcctttg
     3781 cgagcaagaa cgttcattct tagtggaagc agtagctcaa aatggtcaat catggatcat
     3841 tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt gcaaaaaaaa
     3901 gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga tttgcttatt
     3961 gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt agaaaaagaa
     4021 aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt atataatctt
     4081 gatgttaaga cccaaagcgg ttttaagata cgtttatccg ggaaaatgag aggaaaagcg
     4141 atcttagatt ttcaaacggc aaaacatcaa aatggcatag ctgtgaaact aaatggtaaa
     4201 tacccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt actgaaaaaa
     4261 gggataaatt atgcaaggac tcatggcgta aaaaaaacaa tccgccgggt caaactggaa
     4321 ttgaaaccag gatcgatcga ctatgcagag tggatcagtc gtcacgagaa acctgatctg
     4381 aaagagcaaa ggaaagaagc acaagggttt gcataccgtc cattgatttc catacttatg
     4441 cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt attggatcaa
     4501 acgtatgatc actgggaact atgtatcagt gatgatgctt ctactgatcc agctatccga
     4561 aagtgtctgg aatcctatca agcaaaggat gatagaatca aagttgtttt ccgacaagaa
     4621 aatggacata tcagtctggc aactaattct gcgttggaaa tggcagaagg agaatttata
     4681 gcgctcttag ataatgatga tgaattaccg ccatttgcat tatatgaagt ggcaaaagtg
     4741 ctaaacatac atccagaact ggatttgatc tacagtgacg aagataaaat cgatgcagat
     4801 ggcaatcgct ttgatccgca cttcaaagca gactggtctc ccgatacatt gatgggaaat
     4861 aattatatct ctcacttagg cgtatatcga acaagtattg tgaaaaagct tggcggtttc
     4921 cgtaaaggct acgaaggctc tcaagattat gatttggtcc ttcgtgtgac agaacaaatc
     4981 cctgcagatc atatttacca tattgaccga gtcttgtatc attggcggac gatcccagga
     5041 tcaaccgcaa gtaatggtga agccaaaagt tatatctatg attctggagt gaaagcattg
     5101 acagatgccc tgtctagaag gaatatcaaa ggaagcgtac gtcctggcag aatttctgga
     5161 ttttatgaga ttgcttatga cgtcttgcaa gaggatttgg tcagcgtgat cattccaacg
     5221 aagaatggct atgaagatct aaaaacttgt gtggattcga ttatcgaaaa aacgtcttat
     5281 ccaaattatg aaatcattat tgcagataat ggaagtacag atcctaaaat gcaggaatta
     5341 tttgcagaat acaagcatca gctgaaagac cgatttatcg ttgaattaat tgacattcca
     5401 ttcaattatt cacgtatcaa caatctggca gcagaaaaag caaacggcaa atacttttta
     5461 ttcttgaata acgatacaga agtgattgaa cctgactgga tgacagcgat ggtttcttat
     5521 gcacagtttg atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga tgatacaacg
     5581 cagcatgcag gagtattatt aggaatcggt ggtgttgcag gacatgcatt gaataactat
     5641 gatcgaacac attgtggtta tttcggccga ttggtcattg acgtcaatta cttagcagtc
     5701 actgcagctt gtatgatggt caaggcagct gacttcaatg ctgtcaatgg atttgatgaa
     5761 acattagaag tagcattcaa tgatgttgac ttgtgtttga aagtttatga actgggtcgt
     5821 tacaatgtct atgcacatca agcagaattg tatcatttcg aatctaaatc aagaggttat
     5881 gaagatacac cagaaaaaca aagacgtttt gccggggaaa taaaaaaaat gcaagataag
     5941 tggccttctt atattgctca tgacccattt tataacgata atctaactaa agaaggaatc
     6001 ggcgattttt cattgagacc tgattgatta aaagaactgt attttcattt ttattgatga
     6061 aaatacagtt tttttagtat atgaaaattg taacttgtga ccgaagagat gataggaaga
     6121 acacccattc agtaggaaga atgtgatacg atttttgtcc aactctctgt taggcagaaa
     6181 tatacatata aacatacaat agtcaccttt tctagtttct tttaatttat tttacaattt
     6241 ggttattcac taggttttta aactttgcta tggtaagatg atagaggcat tattttatgg
     6301 ttaggagtgt atatatgaat aaaaatgggg agtggaatgc ggcacgtagg atattcatta
     6361 tcatcatgga tgtacttgta ttgaatgtgt cgatctatct tagctttttg ctgaagttcc
     6421 aaggagaagt cccagcaaga aacttagaga cctttcagca ttcagcagtt tttatttcca
     6481 ttatctttat tgcgttgaat attttgttag gagcttatgt attctatagc cgagtgatca
     6541 gtgatatcgt cttcattact gttattggac aggtgctgat agcattgggg atcatggtag
     6601 ttacttttgc tggacgttgg tttgcatttc cgcgtggcgt catcgtgctc tcgtttatac
     6661 ttggcacgat attacttagc gtttatcgga tagttgttta taaactatac ttaagagtca
     6721 gtcgtgataa gaaagtcctt atcgtaggtt tggagaaaga tgtcgcacca gctatccaaa
     6781 actttcaatc taagaaaaac aacaaacata aagttgaagc agtagtgatc gacaagtatt
     6841 acgaaaacac aaaaaaattg attgatgata tcgatattgt ctacttggct tctaaaatcc
     6901 cagaagacga aaaaatcaat atttatcaat tagtaactaa aaaagagaag aagctatttt
     6961 taaatacaac ttttgaaaat ttaacgatga tcaatccgaa catcatgaat ttcgaagatg
     7021 aaagtatcat tgaagcttcc ggtttcaaaa ttcagccgga atatgaatta ttcaaacgtc
     7081 tttttgatat catcgtttcg cttattttac tgattcttgc ttcacctttt atgctaatca
     7141 cagcgatttt ggtaaaaaca acttcacctg gaccaattat ttataaacaa gtacgtatta
     7201 cgaaaaatca aaaggaattt tcaatctata aatttcgttc catgagtgta acagctgaag
     7261 caaaatcagg accggtcttg gcaaagagca atgatgcacg tgtgacacca gtaggcaaat
     7321 ttatccgtgc cgtacgtttt gacgaattac ctcaaatctt taatgtattg aaaggcgata
     7381 tgtcgatcgt cggaccacgt ccagaaagac catttttcgt tgatcagttc aatgaagaaa
     7441 atccttatta ttatttaaga cacaatgtac gtgcagggat taccggatat gcacaagtat
     7501 acggaaaata tgtatcagat tataatagta aactacgttt tgatttgttg tatatcaaaa
     7561 agtattcctt actattagat ttgaagattt tacttcaaac aatcaaaatc ttatttgata
     7621 agatgtcctc acaaggacta gatgaagatg aaaatatccg ttcgttagca gaaatcgact
     7681 tttcacaaga gaaaatttat cgctagatgg gaaaaatttt gagcaaagaa gtaggagaga
     7741 acattggaaa ataaaaatat aagtatgctg ctttctttat atgataaaga aaaaccagag
     7801 tatttaaagg aagcattaac tagtattttt gagcaaacag taatgccaaa agaaattgta
     7861 ttagtttatg acggccctat aaatgaaaaa cttcaagtag ttgtcactta tttccaacaa
     7921 aaagatcctc acttgtttac agttgtaaaa ttagaaaaaa atcaaggatt ggggattgct
     7981 ttagcaattg gattagaaca tacgaaaaat cgcttagtag ccagaatgga tacagatgat
     8041 atcatggaac cccaacgatt agaaaaacaa ttagagatct tccaaaaaga tccagaaata
     8101 gcgattgttg ggagtaatat tgaggaattt actggcacgc ttagcaatgt tatcggaaga
     8161 aggattgtac ctgaatataa tgatgaaatc cgtgtgtttt cgaaaagaag aaatcccttt
     8221 aatcatatga cagtcatgtt tgataaaaat gctattttag aagtgggaaa ctatcgccca
     8281 ttaattggat ttgaagatta ttatttgtgg gtaagactgt tgaaggcggg atataaaggg
     8341 tataatatcc aagagattct agtccatgct agagcaggtg ctgacatgta tgctagacgt
     8401 ggaggaagta aatatctttt accaggaatt aaaggccgat atcgcgtatg gaaagatggt
     8461 ctaggatcac taaaagattt tctgatagtt atttgcggac atcttgtaat tagtttatta
     8521 ccgaataaaa ttaggggaaa agtatatgaa tcaaaactta gaaaaacaaa ctaaactttc
     8581 tataatcata cctttttaca atgcaaaaaa tactttaaaa agagctgtag acagcgtctt
     8641 aacgcaaggt tatcccaata ttgaaatctt gttaattgat gatggatcta cagatggtag
     8701 tagtgacatt ggtgacgaaa ttgctaatct agagagtaaa gtagcatgtt atcacttgga
     8761 aaacgctggt ccatcagttg ctaggaattg tgggataaaa gaagcaactg gaaatttggt
     8821 aggattttta gatagtgacg attatttttt acctgattgt tttaataatg ttttgaaaga
     8881 ctaccatagg aattaccccg atatcattgc ttttggatta agtaaaggaa aaaatataga
     8941 aaatgcaaat ttatttgttc ccgaaaagaa gattggttgt tcaagtgaag aagcaattga
     9001 gcaaatgttt agaagtaagg cagtggattt ttacgtatgg aataaatttt ttaaaaggga
     9061 attattcaat catatagttt ttccagaaaa taaattatat gaggatatgg ttcctatgta
     9121 tgaagctttt agagcggcta gtaaaattga tattttgcca ttttcaggta ttttttatta
     9181 tcaaaataat gatagcatcg tttatcagtc ttttaattca aaacagtatg ataatatttt
     9241 gcaaagaaaa atattagttg atgaagtaaa gaaagattat cctcgactta ttaatttggc
     9301 caatgctcga ttagtagacg gatacctttc aacagggttt aaaattactg gcaagaatca
     9361 aaatcagaaa gaaaagcaaa aatattataa tcagtctaaa agagaaataa aaagtatata
     9421 ttcaaaaata agaagtaaca atgagatatc aattgctaaa aaattagctt
//
LOCUS       DACEUF010000092         9321 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.096, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000092 DACEUF010000000
VERSION     DACEUF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.096"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(52..720)
                     /locus_tag="IXG20_002267"
     CDS             complement(52..720)
                     /locus_tag="IXG20_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1610445.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            854..1141
                     /locus_tag="IXG20_002268"
     CDS             854..1141
                     /locus_tag="IXG20_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610446.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1557..3095
                     /locus_tag="IXG20_002269"
     CDS             1557..3095
                     /locus_tag="IXG20_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ1610447.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3092..4057
                     /locus_tag="IXG20_002270"
     CDS             3092..4057
                     /locus_tag="IXG20_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1610448.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4044..5366
                     /locus_tag="IXG20_002271"
     CDS             4044..5366
                     /locus_tag="IXG20_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1610449.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5429..5533
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5607..6350
                     /locus_tag="IXG20_002272"
     CDS             5607..6350
                     /locus_tag="IXG20_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1610450.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6365..7357
                     /locus_tag="IXG20_002273"
     CDS             6365..7357
                     /locus_tag="IXG20_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1610451.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7369..8106
                     /locus_tag="IXG20_002274"
     CDS             7369..8106
                     /locus_tag="IXG20_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1610452.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8096..8818
                     /locus_tag="IXG20_002275"
     CDS             8096..8818
                     /locus_tag="IXG20_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1610453.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8902..>9321)
                     /locus_tag="IXG20_002276"
     CDS             complement(8902..>9321)
                     /locus_tag="IXG20_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ1610454.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3054 a         1605 c         1968 g         2694 t
ORIGIN      
        1 gtttacataa tgatatttct gtaaattaaa taaagtagtg aaaaataaga atcagtgctc
       61 taactgttca agaggatcaa gtggttcaaa tgtttctgta gtctgttttg cttccaattg
      121 gggcaaaaca gatccaattt ccagatactt cactttattc aatgtataag atagaataga
      181 acagctaagt aatgtataca gaaaatgtgt gagatccaaa taaagcaagg agacaactaa
      241 aataccaaaa gtaatcaaga aaagactttt gccaatctta atatgaaaat atttttcaat
      301 ggcttgataa atgatatctg ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca
      361 accaatgcca gaaagaatac ctgtcatcaa actattgatc agtagactat ctacagcaaa
      421 atgtatatga aggctctccc ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat
      481 cgtccggaga aaagcgtgtt ttccaaaaag aaacaatgag ataagcaata aaggtaaatt
      541 aaaaagcagt gaagtgacag aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat
      601 accagtaacc ccaccatctg ccagcttaga tggaatattg attcctgtca cagctaatcc
      661 gtagattcct gcgcctaaaa tgattccgca caattctgta aataaattta attttttcat
      721 ttctagcctc ttttcttttg aaaattttca gcaacttctt tattatactg acttatactc
      781 ttacttcaag aggaaaatga aaaaaaatta tttttggctg tttcttttta tttatcaaat
      841 aaggagtgga aaaatgcgag cagcagactt attgacaatt cttacaaaat cacccaaaaa
      901 aacagagtat caaggagttt ttttggctca agaacaaaaa ctggtgcctg tgacaatggt
      961 ccagataaat aaagcaaatc aatttatctt tttatttgaa cacggcaaag caccacttgc
     1021 tatgaaagac atctttcttt ttttgatgaa gaaccgtgag aaagctgttt attatcagtc
     1081 aaaagaagaa ctcattcctc tttatggagt gaaagagata gaaaataaag tggtgatata
     1141 agtaaaaata agaaaacaag acgtttatga tgtcaaaaca accatttttt acatttttgg
     1201 caaaaaagtg tcatgataca gtataatatt cgttgaacag gccaataaag gtgttctccc
     1261 ccacgagatt tctactttat tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa
     1321 aaaagatata gctgattcca agtgacaatt tcgtatagat ttttatggta ttcgtagtga
     1381 aaaaacgaga accaaaaagt tgatccgact ttgttgcttg gattcttttt ttgtcagcat
     1441 atttagaggt atttttttct tttttttgtt cgtgctgtaa ggttaaggca agacaagtta
     1501 gttgggaaaa aggaaagaaa tgtttatact taaagaataa cagaaaggaa ggtcgcatga
     1561 ataattttat acaaacatta agtgaaagaa aaggcgagtt gctggaagct gcatttcaac
     1621 atctgtccat ttctctcatt tctttgctga ttgcagcact gattgctatt cctttagcga
     1681 tttgggcagc caatcataaa aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc
     1741 aaacgattcc ttcacttgcc ttattaggtt tacttatccc atttgtaggg atcggaactg
     1801 ttccagcatt gattgcactt gttatttacg ctttgcttcc gattttccaa aacacgtata
     1861 ttggtctagc tgaaattgac ccgtctattg aagaagcagc ggtagcattt ggtatgtcca
     1921 gaatgagacg cttgctgaaa gtagagcttc ccatcgcatt acctgtcatt atctcaggta
     1981 tccgaactgc cttagtttta atcatcggta cggctacttt ggcagcattg atcggagcag
     2041 gtggattagg gacatttatt ttgttaggta ttgaccgaaa tacacctgtt ttgacattga
     2101 ttggtgcgat cagttctgcc ttgcttgcaa ttatattcag cggactgatt cgctggttgc
     2161 agcataagaa acctcgatat tccatcatta ctttagcagc agtggtgtgt ttatttggcg
     2221 gcgtttcttt atatcagtct gccttgttta aaaatgaaac catcacgatt gctggaaaat
     2281 taggagcgga acctgaaatc ctgatccaaa tgtataaaga attgattgaa gacaacacaa
     2341 aaacagaagt tgaattaaaa ccgaattttg gcaagacgag ttttttgttc aatgcactgg
     2401 atcaaggtca aatcgatatc tatcctgagt tcacaggtac tgtcctagaa agcttggtga
     2461 aggtacctga agcaatgaaa agtgctgatt tgacaaaaga aaaaacatat gacgaagcag
     2521 aagaattatt gaataagcaa ttcgatatga cattattaaa accaatggct taccaaaata
     2581 cgtatgcctt ggcagtcaag agatcttttg cgaacgaaca tcaacttaaa aagatttccg
     2641 atttggcagc tatctctgat cagatcaaag cgggattcac actggaattt attgatcgtt
     2701 cagatggata taaaggaatc caagaagcat atggcttgaa tttttcaagt gtacaaagca
     2761 tggagccaag tttgcgttac caagcaatca ataatggaga tgtcaatatt gtggatgcct
     2821 attccacaga tagccagctc aaggagtacg atctagtgac tctagaagat gatcgagggt
     2881 tattcccagc ttatcaaggc gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg
     2941 aaatcgttaa agcactgaat aaattaagcg gaaaaataac agaggatcaa atgattgata
     3001 tgaattacca agtcaatgtc gaaaagaaaa agccgacaga agtagcacga atcttcctga
     3061 aaaatgaagg attagtaaag gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt
     3121 gcaaaaaaag tatgatggaa agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg
     3181 agaaatcttt gttttggttg gtccttccgg tagtggaaaa acaactactt tgaaaatgat
     3241 caatggatta tctaaaccat cagcaggaga catctacttc aaaggaaaat cgttaaatga
     3301 atataatctg cagaaaatgc ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt
     3361 cccgaccatg acagtcaagc aaaatattga agtcatccct gaaatgttag gttgggaaaa
     3421 gcagaaaaga gcagaccgtg tagatgaact tcttcaaaaa gttggcttat ctcctgatat
     3481 ttatcgagat cgtatgccta gagagttgtc tggaggagaa cagcaacgga tcgggatcat
     3541 ccgagctatt gctgcaagcc cagatgtgat tttgatggac gagccattta gcgcattaga
     3601 tccgatttca agaaactcat tacaagaatt ggttttgtcc ttacacgaag aattgggcac
     3661 gaccatcgtt ttcgttacgc acaatatgga agaagcaatc aagctaggtg atcgtattgc
     3721 atttatgaag gatggagaaa ttatccaatg tgacacacca gagcagttat tgatgaatcc
     3781 taaaaacgac tacgttcggc atttctttga tgaaccgaaa cagacgaaag agtggcgagt
     3841 agaagattta gtcgtcaatg gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca
     3901 agtttgcttt gatacgccta tgaaagaagt atattcgctt ctttcggtgt atccaagcat
     3961 tgtgatagta gaaaagcaaa gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt
     4021 tttaagtcga gaggaggaag gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg
     4081 tgagcggctt aagtgctgct tatggattga aagaagcagg taaaacagta ctagtggtcg
     4141 aagaagatct atggggcggg acttgtccta accgtggatg tgatcctaaa aaagtcttgt
     4201 taagtgcagt agaggcacgt aatcgggtaa aacaattgag tggaaaaggt tttaacgaga
     4261 tcccaactgc caactgggaa gaacttcaaa aattcaaacg aacatttacg gatccagtcc
     4321 cagaaagccg aaaaaaacaa ttagcagaag ctgaaatcga tcatctttca ggaaccgctc
     4381 gttttttgga tgattcatct attgaagtca acgaagaagt cttccatgcg gattacttag
     4441 ttttggcaac cgggcaaaga cctacgatct taccagtgga gggaaaggaa tacttgaaaa
     4501 ctagtgcaga ctttttatca ttgcctgtac tgcctaaaga aatcattttt atcggtggtg
     4561 gttatattgc ttttgagcta gctacgattg ccaatgctgc agggagcaaa gtaaccattg
     4621 tccatcataa tcaacggcca ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc
     4681 atcaaatgga agcatctggc attcagtttg cttttggtgt tgaaacccaa aagatcattt
     4741 cagaaggtac tcgctatcgt ttagtcggaa aagagacgga attagtagca gatatgattt
     4801 tttgtgcgac tgggagacag ccgaatacag aatctttagc acttgagcaa gcgaatatcg
     4861 tctttgataa acacggaatt gcagtcaatg attatctgca aacgagcaat ccaaaaattt
     4921 ttgcgtgtgg agatattgtt tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg
     4981 aaggaaatta tgtagctaag cggataacgg atgcaacaag cgaacctatc aaatatccga
     5041 ttatacctac gatcgtctac gccagtccta aacttgctga agtaggagtt acaaaaagtc
     5101 acgcctcttc gtcagatcaa gtggtagaga tggatcttac cagttggttc acctatcatc
     5161 gtgtcaatga accagttgct aaggctgaat tgacctttga ccagcaaaat tatttgattg
     5221 gtgctgcagt gattagcgag caagcagacg aattgatcga tgatttgaca ctcgtcatca
     5281 atcaaaaatt gactaaaaaa gagttggata gctatatcat ggggtatccg acattggcaa
     5341 gtgatctctc ctatttattg aaataatcag catgataact aaagcgcttg ttgctttttc
     5401 cttcttgtgc tacactttaa aaaatgaata cctgtttctg gggatgtcgt agacgacatg
     5461 agagtaagtt gtgaacttag acccattgaa cctgtcagtt aatactggtg aagggagaaa
     5521 catagcagac gcttgaagcg ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt
     5581 catattagaa gaaaggaggt aacaaaatga agaaaaatct ttatccagca gtcagtatgg
     5641 cattcatttt aattatttgg cagattgttg tcatagtttg gaaaatacct gcctttattt
     5701 tacccggacc tgctgctgta ttttctgcat tagcggcaga tgcacaagta ttactttccc
     5761 attctgtcac gacattaaat gaagcagtgc ttggtttggt aatagctagc gttctttctt
     5821 ttattactgc cttggcaatg gattattgga agtttttaga aatgtctttt tatccgttac
     5881 tagtgatttc acaaatgttg ccaatcatgg tgttagggcc attacttacc ctatggtttg
     5941 gtttcggtat gcttccaaaa gtgatccttg ttgtcttgat aagctatttt ccgatcgtag
     6001 tttctttttc ggatacattg aggaaagtat caaaagagca aatcatcttt ttgaaaacga
     6061 tgggggcaga tacagggaag atttatcgaa tatataaaat accagcgggt ttatccggct
     6121 tcttttccgg tttaaaagtg gctgctactt attgtgtcag cggagcagtc gttggagaat
     6181 ggctaagcgc tcaagcaggc ttagggtatt acatgatacg cgtaaaaaac agctaccaga
     6241 tcgataaagt ttttgctgcg atcatttgtg tcattctatt gagtttattg ctgaatggcg
     6301 cttgttcatt attgaaaaaa gggtattatc gtatgcttta tattttttaa gagaagggga
     6361 tcaaatgaaa aaattatggc ttttattacc gctactgttg cttttatccg cttgtggcac
     6421 tgcaaaagaa acaagtagca aacaagaaat aaaggatttg aaaaaagtga cacttgtttt
     6481 ggattatgta ccgaatacta atcatactgg catctaccta gcaaaagaaa aaggctatta
     6541 taaagaggct ggtttaaatg tccaaatcat cgaaccagga gataattcga caagtattgg
     6601 tctagtaggt gcggacaaag cacaatttgg tgtaagttat caagaagatg tcacttatgc
     6661 tcacgcagat ggacagaata taccagtcaa agcaattgct acagtgatca aacataatac
     6721 ttctggattc gccaccctat cagacagtaa catccattca ccaaaagatt ttgaaggaaa
     6781 aacatatgca ggttggcaaa gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa
     6841 agacggcggg gacttttcaa aactgacaat ggtaggaagc aatggagaag ggccagaaag
     6901 cttaggaaaa tcctcagata tccaatggta ctttgaaggt tgggatatga tcaaagcaaa
     6961 agaagcgggt attgaactaa actatatccc attgaaagaa ttagatgaac ggttagatta
     7021 ctatactccg gtaattatta cgaatgatca actgatcaaa agtgatccag aattggtaca
     7081 gtcttttatg gatgccacga aaaaaggcta tcaggaagct attaaggatc caaatgacag
     7141 tgctaaactg ttgcaaaaat atgcaaaaga aaatgatcgg acgtttttag aagaatcaca
     7201 agcattcttg tcaaaaaatt atacggatga tcctgagaat tggggattga tggaagaaaa
     7261 agtttggcgc aattatacag cttttatgca ggaaaacgga ttgatcaagc aagatgtacc
     7321 ttcgcaagaa ctattcacta atcagtttat aaaataggag tgatagatat gtcaatccaa
     7381 atcagccact tgaatgtagt cattgaaaac ctgaaaatat tagaagacat ccatttcact
     7441 atccctaaag atacctttac atcgattgtc gcgccaagcg gagccggtaa gtcaacattg
     7501 ctgaaaactt taacaggagt ccagcctatg acttctggaa cgatcaaagt agacgatcaa
     7561 aaagtaactg gtctccatac agcatttagt tatatgccgc aagaagatat gttacttcct
     7621 tggctgagcg tctatcaaaa cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat
     7681 gaagaacaag taagggatta tctggatatc tttgggttaa acggctatga acattttttg
     7741 ccggaacaat tatctggcgg gatgaagcag cgaacggcct tgttacgaac aatcatgaat
     7801 ccttcttctt acttgcttct tgatgaacca tttggagcgt tagatgctat gaccagaggg
     7861 cagatgcaag actggatgtt gaagctgcct aaaaaagcaa agcggactac tttacttgtg
     7921 acgcatgata tcgaagaagc aatttattta tcagacagga ttcttgtgct ttccgcccgt
     7981 ccggcacatg tgattgctga gattcaagtg cctgaaaaac gacggtcacg tgaatggcta
     8041 ctgcagcaat cagagttgaa gcaaacgatt tatcagttat tggcaggtga attagatgtt
     8101 aagtgatgct cattgtcatt tagatgggag tcagagttta gcactccttc agcaggaaca
     8161 cacgattttg acgatcatca attgtgattc gccagaagaa tggaaggaaa acagacagct
     8221 tgcagcaagt aagactcaag ccttaagtta cggtatccat ccttggaaag cagatagcta
     8281 tacgtttgaa caagttgagc cttttttgaa aaaagcaagg atcatcggtg aaatagggtt
     8341 agataatatt tggacgaatg taccgatgac tacacaaaaa aaagtatttg agcgacagct
     8401 tgcttttgca gccataaatg agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga
     8461 gatcttaaat tatatccaag cttaccctaa tcactatttg atccattggt attcttcttt
     8521 ggactatcaa caagactata ttgatttagg ctgttatttc agcattggtc ttgatttgaa
     8581 aaagaatcca gcagtctggc aactagctaa agcagtccca atcgaccact tacttattga
     8641 aacggatgga ctacatgcag cagagtgggc tttagggggg attttttcag aaaaagatta
     8701 tgtctcactg cttgaagaac agttaaagac aatcgctgag atcaaagagc tatcagttga
     8761 agaagttaaa aaaaatactt atcaaaactt ggaacgtttc attagattag gtaaataaaa
     8821 aagagatgtg acacaactag tgtcgcacct ctttttggct ttctttgtat aaaagtaaac
     8881 tgtttgatta ataaatgaat cttagtacca accgtttgct tcccagaatg ctttcgccgc
     8941 ttcccaagaa ccataacgtg aagtaacata ttgttctgct actctttctt ggtttgcagc
     9001 agagtagtca ccatttaagt aagaagaatc taattggtaa cgtccgatgt aacgtccgtt
     9061 cgttgctgtg taagaaccgc ttgattcttt ttgtgcgatc cattcttttg cagagcttgt
     9121 gcttgctgga gtagcagcag tttctgttac agcaggagca ggttgtgttt cagcaacagg
     9181 cgctgcttgt ttttccgttt gaaccgtttc ttgtacaaca ggttgttcag cttggactgg
     9241 tgcttcttga acggctgctt gaggttcagc agctgcttgt gcagagcctt ctgttgggat
     9301 tgttaattgt tgtcctgagt a
//
LOCUS       DACEUF010000093         9292 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.097, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000093 DACEUF010000000
VERSION     DACEUF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.097"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..384
                     /locus_tag="IXG20_002277"
     CDS             <1..384
                     /locus_tag="IXG20_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610455.1"
                     /translation="NNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRL
                     LLGRCRTCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNY
                     FLLNQSIIGVKTLAYLSGMGRNRRF"
     gene            702..1292
                     /locus_tag="IXG20_002278"
     CDS             702..1292
                     /locus_tag="IXG20_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAQ1610456.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            1319..2629
                     /locus_tag="IXG20_002279"
     CDS             1319..2629
                     /locus_tag="IXG20_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1610457.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            2626..3345
                     /locus_tag="IXG20_002280"
     CDS             2626..3345
                     /locus_tag="IXG20_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1610458.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            3360..4445
                     /locus_tag="IXG20_002281"
     CDS             3360..4445
                     /locus_tag="IXG20_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1610459.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            4581..6113
                     /locus_tag="IXG20_002282"
     CDS             4581..6113
                     /locus_tag="IXG20_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1610460.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            6291..6512
                     /locus_tag="IXG20_002283"
     CDS             6291..6512
                     /locus_tag="IXG20_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ1610461.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            7094..7711
                     /locus_tag="IXG20_002284"
     CDS             7094..7711
                     /locus_tag="IXG20_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1610462.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            7708..8238
                     /locus_tag="IXG20_002285"
     CDS             7708..8238
                     /locus_tag="IXG20_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ1610463.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(9104..>9292)
                     /locus_tag="IXG20_002286"
     CDS             complement(9104..>9292)
                     /locus_tag="IXG20_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1610464.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT         3309 a         1010 c         1777 g         3196 t
ORIGIN      
        1 aataataatt cattgacaca taattttaga gataatgagt tcgattgtaa tattgaatta
       61 gttaaaaaaa ttgaaggcga tttaagctat atattagaaa atgagatatt tgtaaacaaa
      121 acaaggagac ttttgttggg acgctgtcgt acttgtattc aaaaggcagt tagatatgct
      181 agttttaata aagaatttga tttaaactcc tatttgtcta agatacttat gaacgatgag
      241 ttgattgcct gtattcactc atacccatat aaacaaaatc caattaaaca acgaatattt
      301 aactattttt tactaaacca atccattatt ggggttaaaa cattagcata tttaagcgga
      361 atgggtagaa ataggagatt ttaatcataa aaaactgtac tatagtgaat ttttggagat
      421 ttatattcca gttatatatt aatgattgtg atacttgtac tattgtaaaa attcgttagg
      481 tttaaataac tggaaatatc ggaaaatggg cattatacat cacggacatc ccttcttttg
      541 gaaaaaatgt tacgattttt ataagtaata ttgtatccgg tggaatcaac gatgaagata
      601 acatatccat cgttgtagat tcagtagcat tgaatgatgc tcttgttgca agaaatccga
      661 ataaaattgt taagtataaa ttgaattaat aggagatatg aatggaaatt agcagtaaag
      721 aatatttaaa agcggttctt cgtatggaaa agaatctata tatagaatct aaaggacaac
      781 acacaaaatt actaaaaata atgaaatcac atccggacta caacgcttgg cggtatactc
      841 gtcgaatgag aattacatct tattattatt caaagagaaa gaaaaatatc atttatgcga
      901 ttatgtatat tgtgtcttgc agaagaatga atagtttggg aagaaaatgt ggtatagagt
      961 gtggtgaaaa tgtatttgat gaaggtgtaa gattttacca tacacaagga attgttatta
     1021 atggtaacgc cataatagga aaaaattgtt acttatatgg gaataactgt attggaaatg
     1081 atggaatcga tcctaagtgt cctgtaattg gaaataatgt acgtgtatgc gtgggtgcaa
     1141 agattattgg taatgtaaaa atagctaaca atgtagttgt tgcggcagga gctattgtta
     1201 tcaaagattg tctagagaat ggtgcaattc ttgcaggtgt accggcaaaa ataatagggt
     1261 atgttaattt aacaaaagta tttttagaat aatttttagc gaaagaaggt gaataacgat
     1321 gcgtatttca ataaaaagat ggtcaggagc agattggaca ttgttcacgt tggcattttt
     1381 tgcaatctcc atagttcttg tatatacttt taacgcccca agagcaattt tatatttggg
     1441 agatataatt aatatctttg tgttattaaa tgcacttagc tttagacgca gaaaaggaat
     1501 atatgctagc aaaaatgaga tgctgatagt tttgttttta ctagtttcta tttgtagctc
     1561 agttattaat ttagaaaata taggattgat tatctggggg atacgaaata acactcgtat
     1621 gttcatagta tatataaatt gtgcaacata tttaacaatt aaagattact ataaagtatt
     1681 cgctataatt gaaaaattat tctggatttc actgccactt tgtttaattg aaagattcat
     1741 ggtcacctat ccaactggaa caattgtagg agatatgata ggtggagttt tttggaatta
     1801 ttcaggttgt aatatgccat tgaatttaat tttgtgtgtg acagttactg ttgttacaat
     1861 cggatatttc aagggagaaa aaaatttcat ccattttatt ttatgtgttt gtgcagcact
     1921 ttttatgtct gcattagctg aattgaaagt ttttattcct gaattattta taatatttgt
     1981 tgcaatagta ttcactttaa aagttgattt taaagtagtg atcaagatat tatcatgcgt
     2041 aatacttgtg tcgatagtat tatccttttt tataacaata tttattacat tgaacgataa
     2101 tggacaaagc tatgctgata atttcacaat tcaaggtctt ttagaatatg cgtcaagaga
     2161 ttctggatat gatggtgttg gtgatttaaa tcgaataact ggtatctcta gagtagtaga
     2221 tgaaatattt aataataatt ttagattagt cctaatggga gtaggcattg gcaatgcaga
     2281 gtttaccaat ttttttgtca gtgagtttta taaatcgttt ggtaatcttc attatcagta
     2341 ttttcatcat atttggttat ttatagaagt tggaatatta ggagtttcga tattcctatt
     2401 ttttataata aagacatttt tcaatgcaaa aagatctttt atgaaggata aagatgcaag
     2461 tcttgttaaa gttattgcaa ttatgtcggt attctttttt gtgtataaca caacacttag
     2521 gggtgaaaca acggggccac tgatttatat gattttagcc atgcctgaag ttgtgaaaag
     2581 gaaagagcat tattatagtg aaaaaaatac aaaaggagag atattatgat tcctaagaag
     2641 atacattact gttggttcgg gggaaatcca cttccgaaaa aagatagaaa gtgcatagaa
     2701 agctggaaaa agtattgccc tgattatgag attattgaat ggaatgaatc aaattatgat
     2761 ttaaataaga accgttatat ggccgatgca tataaagaac gtaaatgggg ttttgttcca
     2821 gattatgcaa gatttgatat tatttataat catggtggat tttatttaga tacagatgtt
     2881 gagctaatta agtcgttaga tatacttcgt gaaaatcatg cctatatggg atttgaaggg
     2941 gaaatctggg ttaatggcgg tattggtttt ggtgcagaag aaggacattc tgttattaaa
     3001 ggtttaaggg atatttacga ttcattaagt ttttatcaaa atgatggtac attgaacttg
     3061 aaaccgagtc cgtattatat tacagattat cttgaatcta ttggattggt tcgcgataac
     3121 agcgaacaaa taataaatgg cctgcatatc tatcctacgg attattttgc tgccaaggat
     3181 tatgatactg gaaaaattta taaaactagt aatacaataa gcattcatca atataatgca
     3241 tcttggaaaa cacctaaaca aaaatttgtt tatagattaa aatcaacaat tggacaaaaa
     3301 aaatttaaca ggttacttgc actgaaaaaa tttttacact tataaaggag aaaagtactt
     3361 tgaagaaaat ttgtttcatt agtggtgtaa tttctcgttc tgggggaact gaaagagtag
     3421 ggagtattat tgccaatagc ttgtctgatt taggttatga tgtcactatt ttaagctttt
     3481 ggaatagtgg agagccttat tttgggttag ataagacgat taaggttgat tatatgttaa
     3541 atcctaaaac ggagggaaaa ttatatagaa cttatttata tccggtttgg aaattgcaca
     3601 agtatatatg caaaaatgaa tttgatatag taattgatat tgatactttg ttgtctttgt
     3661 atactacaag ggcaattaaa ggaacaaagt gcaaattgat ttcttgggaa cattttaatt
     3721 actggacaat ggaaatgctg aaagaaaaga agagattcaa ggctaaaaaa ttgataaaaa
     3781 aatatgcaaa gaggcttgtg gttctcacag aagaagatcg tcaaaaacat attgatgaat
     3841 atagtttatc agaagatttt gttataacta tgccaaatcc gtgtatatct aatatcgagt
     3901 acgaatatca ttatgataaa aagagatttc ttgcagtagg gcgattagtg gaagagaaaa
     3961 attttgctga catgataagt gcttggaaaa ttgtagaaaa aaaagtagat gattgggatc
     4021 ttatcattgt aggaaaaggt gaattagaaa aagatcttcg ccagcagata aaagatttaa
     4081 atctgcaaag agtaactata gcagggcatt cagaccatgt ggaggaatat tataaatctg
     4141 catcttgtta tgtgcttagt tctgtttatg aaggatttcc tatggttata ttagaagcac
     4201 agtcttatgg attaccagtt atttcttatg attgtaaaac tggtccgaga gatttggtac
     4261 atcataattt taatgggatg ttggttgaag ataagaatat agaccaatta gctaagagca
     4321 tgataatgtt tactaagaat actgatttag caatgagaat gtcactgaac gcatataata
     4381 atgtacaaaa atttaatttg aaagaaatta caaaacaatg ggttgctttg attgaaaatg
     4441 tatagttttt tgtggttgtc caaacatgaa aattagcgta attaatcaaa gagctagaag
     4501 aaacctatta catttcaaga ttttagaaat tatatagtat taggtaacaa taaaaaatag
     4561 aaagtttgga gccatatgat atgataatta atcgtaaaca aaatgctaaa aggaatataa
     4621 ttactggaac aatttttaag ttatatcaga ttatcattcc cttttttatg cgtacactta
     4681 tgatttatca gatgggaatg gagtatgctg gattgaacaa cctgttcaca tctatatttc
     4741 aaatgttaaa tttagcggaa ttgggaatag gtgctgcact aactgtaagt atgtataaac
     4801 ctatagcaga agacaataga aaagagattt gtgctttact gaacgcatat aaaaagtact
     4861 tttcaattat tggatggata gtgtttacat taggattgtt atgcattcct tttttgagat
     4921 ttttagttaa aggttcgatt cctggaaatt taaatatgac agttttgtat ttaatgtatt
     4981 tgataaatac tgttttgtcg tattggttat tttcatatag aaagagtcta atatatgcac
     5041 atcaaagaaa tgatgtaatt aacgtgtcta tgctaattac ttttacattc caatttgtaa
     5101 tgcaggcata tgttctgatt ttcttaaaaa attactattt gtatttattg gcttctattt
     5161 tgggacaaat agttcttaac ttaatgtgtg ctgttgtttc tcaaaagatg tatcctgatt
     5221 atactcctgt tggtgagtta gagaaagaaa ctattgacga tattataaaa aaagtaaaag
     5281 gattagttac aaacaaggta ggcggtacaa tattacgatc ttcagattca attattattt
     5341 catctttctt aggtctttca gtacttgcga tttatcagaa ttattacttt attatttctg
     5401 ccataatgag tatatttgca ataatttatg aatcatgtat agcaggtata ggtaacagtc
     5461 ttattgtaga aagtcaagaa aaaaattata tagatttttg tgatattaca acaattatag
     5521 gttggtttct ggcaattagt tgtgcatgct ttattacatt atttcagccg ttcatgatta
     5581 tctgggtggg aagtgataat cttatgagta tttcatttgt tatatttatg atagttcatt
     5641 ttttcttata tgaaattgat cagttaattg ggttatacaa agatgctgct ggaatatggt
     5701 acgcagacag gtttagacca ttaattacag caatgataaa cattgtatta aatatcatcc
     5761 tcgttaattg gttcggatta tatggagttt tggcggcaac cattgtttca gtattgttgg
     5821 ttgatttacc ctggttatta tataatgttt tccaaaatat atttaaattt gaatcggcag
     5881 gtaaatatgt ttggaagctc ataagtttgt gtgtaatagc ttttgttgct tcttatttaa
     5941 gttattttgc ttgttcgtat gttaatatcg gaaatttcaa agtgataagt ttagtgataa
     6001 atttagttat atcggttgtc atatcgattt tagttattct tctgtttaat atccgtaata
     6061 agaagtttaa gtatattttg aaagttctta cgtcggtatt ggtaaaaaga tgagaagaga
     6121 catcagaatg atatataaag taactacaat ttttaggctg ccgaataggc gaacgatgta
     6181 tagtttttgt tccaacgaag actcttgttg aaattggaga tgatatgaag gagttacaat
     6241 tttgactcat gtttatgatt gatcggtttt aaaagtagta tggagaagct ttgggatcat
     6301 cggagaggat caaaataggt aataatgttt ttataggaat gaagtctaga atactaaagg
     6361 gtgtacatat aggaaataat gtagttatag gtgcaaatag tttagtaaac aaggacgtat
     6421 cagacgattg tgttgttgca ggaaatctag caaaagtaat tatgtcccta gatgaatatt
     6481 ataaaaagat aaacaatatg aagaatccgt aaaacttgtg aaactatacc gagaataata
     6541 tgggaaaaat attgatgaaa aagcattata tgagttttta gggctgttta cagatggaga
     6601 tgacaatttg tcgaagagtt gggagcaatg caaaatttag taggggatta taaacagact
     6661 gacaaggtat ttaaagaaca tgcacaagta tttcataata ttgaagaatt tttgaatact
     6721 gtgagatagg agttgctaca ttgagaaaag tatgtatttt tccaatgtaa aagattacga
     6781 gtttgaaaac gacaccataa tattattttt taagaagata ttatagagct taggaacgga
     6841 agtttattta aggatgttga ataaaatgaa aaataagatt aggtggatga aaaaggcaga
     6901 tgatttttta ctataattaa aaatattgtt actatgttat caatcgtata aaaatactta
     6961 ctcgttatca agacgattat ttcgaatatt ttaggaaaaa taaattgaat attagagaat
     7021 gtgataatac actctaatat gatcatgtgt aattggaagc gataagtgta tacttgatat
     7081 tggagaaata attatgataa tagtgattgc agaaattatt acagatatat tatataaatt
     7141 atatgaaccc tttgggtttg ctgttttatt gacaatttta ggactggtct attttatgta
     7201 tgcaacagat ggcattgctg caggtgaggg ctataaacgt atactaatgg aactttatct
     7261 tcatttaaag aaggatagaa aatatagaaa agtttgtgct ttgatattct atactgcgat
     7321 gattttattt acaacattaa tcaatcgaac aatttggtta aatcctttat ctgatgttat
     7381 gggagactgg tggatttata agtataatac aacaactgga acaaaagagt tatctacagc
     7441 gtgtttagaa aatattattc tttttattcc atatgtttat atggtaatgt ctattaaata
     7501 tgaaaatata aagaaaccca aaatatggtc ctcaatagta tatggaggaa aaattgcatt
     7561 ttatttttca gcaataattg aatttttaca actttttttg aggctaggaa cgtttcaaat
     7621 ttcggatttt gtatataata cgttgggcgg tatattgggt gtaatccttt ataaactaat
     7681 tttgattaca aaaagagatg gtgaaagatg agactcggat tgataataaa gaatatatta
     7741 aatttaccta aaacgatata cttcaattat aaatttcttc atatttcaga tgcgtggaga
     7801 ttacctattt tggtgcatcg taaagtacgg attacacgca aatcgagagg gaaaattgag
     7861 atagtattac aatctggaaa aatagtttat ttgggctttc cagtattaaa tactgcggga
     7921 aatgaagaaa cgattataag tattgaagga atactaagaa tacaagggcc agcgtttatc
     7981 gcacaagggg ctcatattga cataaggaaa aatgcagttt tagatattgg gtcaaagttt
     8041 aattcgacag gaaaaatgag tataatttgc tttaaggaaa ttcaattcaa agatggctgt
     8101 acagtatcgt gggatacatt atttatggat accgattcac atacgatttt tgaattgaat
     8161 actcatagga ttttgaataa aaataataaa atagttatag gtaaacattg ttggattgga
     8221 gcaaaaagcg tgatatagaa aggtgttcat ttagcagata atattgttgt tgcttgtaat
     8281 tccacggtag taaaaagtca tgaaaataat aatgagatta tagggggggc tccagttcga
     8341 acgttgaaaa gaaatacaag ttggtgtttg aacgagccaa agtgaaacca aataattata
     8401 aaactatgga agtataataa agtttttgag aaatttgaac gacaagaccc taaaaaaatc
     8461 catggaccat tattatggat ggacttccca aaaggaatag ccacttttga gcacgtaaat
     8521 cccctaataa gtggatcgac atcgcttgtg cagcagatga tactatcaag aaaggtgttt
     8581 ataatgtaga acttgaacta aatagatttg taatcaccca ttaggattgt aaagaatatg
     8641 aataggatat atcttaagat gaaaaaattt gcaaattcta atgtatattt attaagggca
     8701 ctttgctgtg tttttttcag agataagatg aaagaaagat ggaagtcttg gagtatacga
     8761 aataaagaaa aatattatat ctgtttaata agttgatatt gaacaaagga ctagtcggag
     8821 tcagcaacac ataagttgtg agtgatgctg actcgttttt ggcagaaaag aatcgaatag
     8881 taagtaggtg ttttgatgtt gcttgacaat ggagagagaa cccaagaaga ttactagcaa
     8941 agcttataga tgttactgta gatttttaac tccagataca gagtttgatg aatattctga
     9001 ttatatcaat aacactataa aaaaggaaat ttccttttca caggagaaag gtaaaagata
     9061 ttttcatagg agaatcaatg ttaattttaa attatatgat ttgtcaaatt aagctagaat
     9121 aaaatgcttc ttgtacatag ctcaaaaata tttcgattgg tgttctgtaa ttcaatgatt
     9181 ttcttggaat atgattgcgt tgattgctga cactggaaat aaatgtctga ttcacttctc
     9241 taaaatctat tgatttcggc agtccattac gacgcagaat cccgttagaa tt
//
LOCUS       DACEUF010000094         8736 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.098, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000094 DACEUF010000000
VERSION     DACEUF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.098"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(479..1066)
                     /locus_tag="IXG20_002287"
     CDS             complement(479..1066)
                     /locus_tag="IXG20_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610465.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            1241..2563
                     /locus_tag="IXG20_002288"
     CDS             1241..2563
                     /locus_tag="IXG20_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1610466.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2718..3494
                     /locus_tag="IXG20_002289"
     CDS             2718..3494
                     /locus_tag="IXG20_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1610467.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3487..4056
                     /gene="rnmV"
                     /locus_tag="IXG20_002290"
     CDS             3487..4056
                     /gene="rnmV"
                     /locus_tag="IXG20_002290"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ1610468.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            4049..4933
                     /gene="rsmA"
                     /locus_tag="IXG20_002291"
     CDS             4049..4933
                     /gene="rsmA"
                     /locus_tag="IXG20_002291"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ1610469.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5384..6013
                     /locus_tag="IXG20_002292"
     CDS             5384..6013
                     /locus_tag="IXG20_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610470.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(6137..7246)
                     /gene="ugpC"
                     /locus_tag="IXG20_002293"
     CDS             complement(6137..7246)
                     /gene="ugpC"
                     /locus_tag="IXG20_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ1610471.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7455..7877
                     /gene="mgsA"
                     /locus_tag="IXG20_002294"
     CDS             7455..7877
                     /gene="mgsA"
                     /locus_tag="IXG20_002294"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ1610472.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            8200..8736
                     /locus_tag="IXG20_002295"
     CDS             8200..8736
                     /locus_tag="IXG20_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1610473.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         2765 a         1543 c         1790 g         2638 t
ORIGIN      
        1 aaattcaaag ggggtatgac actagttgtg tcataccccc tttttccgaa atttgagaag
       61 caattttcgt aaattccttc ttattacttg aagccaatca tataatacag aaacagagaa
      121 ctatccaaaa taaatcctag aaaaagaagt acgagcttcg ctgatttttg ttaatttaga
      181 ataatactaa taggaatcgt aaaaacagaa aaaaagcata atgataaagg gtcgggcaga
      241 ttttaattga ttcatctggt actctttttt attgtcctta cattcataaa gtttgaatgc
      301 aatgacttta ttacatctat atttatatct aacagtagcg ttgatgtgtt aattataagt
      361 tctatcactt tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa atatagataa
      421 aaaaacaatc atcggctttg acttcgatga ttgttttctt tattttgtaa aaatcctatc
      481 acttgatttt atggtaacct tttatttttg tcttcaaacg gcttggtaag agagcagtaa
      541 gcagtgcaaa tgggatcgaa aagacatacc agaaaatcgt acgtccgata ccataatgtt
      601 gatagagctt tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag gagccagcta
      661 taaaccaatg attatcccaa tgcatgactt cactaagtgc catcactcca aaaggataga
      721 aataaaaata gatcccgact aaggtcatcc gccagttctt tgtaaatgtt ccgatattca
      781 ataaaaagaa aatcgctgaa agcaaaatac cgataggcgg aaaaagccca accaaaaagg
      841 aatacaacat cccccacaag atcatcacag gacctttgat caatgcaacg attgcgatga
      901 acaaagtcag caatattagt ttttgtgata acgttgcaac agtcattaat ccaaataaaa
      961 ttaatgtgaa ccaatgccaa tgggactgct gttgttcttt acgggaaaaa tcgacccggt
     1021 tggataaata atctgctaat ttcggataag aacgcttaaa atccataaac tgttcctcct
     1081 tcgtggaaaa ggtaggttta gtatacgaaa aataaaaaag aaatacatcc gtcttaagag
     1141 ggagtttttc actgaaagtg gagaaaaaag aatggtaaat taaaatattc tcagttatac
     1201 tgaaaattat accaaatttc aaagaagaaa gaggatggga atgaaaggca aactaaaacg
     1261 agaaatcaat ctgtttggcg cattagctac cgttatggga accgtgatcg gtgcaggcgt
     1321 cttcttcaaa actgctgctg ttactgctag tactcagtct acaagtttga cattgctagc
     1381 ttggcttttg ggtggtttct taacgatctg tgctggattg acagtggctg aattagctac
     1441 agcaatcccg gaaactggtg gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc
     1501 cagcttttta ttaggatggg cgcaaagctt gatttacttt cctgctaata ttgctgcatt
     1561 atcgattatc tttgcgacac aattgacgaa cttattacaa ttatctaccg attatctttt
     1621 gttgattgca gtgatcaccg ctgtttctgt gacagggttg aatttgttag ggacaaaagt
     1681 cggtgcttct gtacaatcag tgacgttgat tgttaaatta ataccaattg ctgtgattgt
     1741 catatgggga ttattgaccc caggacaagg aactgtccag ttatttccaa tcgaagctgg
     1801 aaaagatgtt acttttgtcg aaggattgag cagtgcgttg ttagcaacgt tgtttgctta
     1861 tgacggctgg ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg aaaaagattt
     1921 gcctaaagca attattcttg gtttgagttt tgtaacagtc gtttatctat tgatcaattt
     1981 tgtttttttg aaaactttgc cgattgatca tcttgccggt aatttgaatg cagcttctga
     2041 agcatcagac gtcatttttg gaggaatcgg tggaaagctg gtcactattg ggatcttgat
     2101 ctcggtatac ggtgcattga acggttatac actaactggg atccgcgtgc cttatgcgat
     2161 ggctttagaa gacgagctac ctttcagtaa acagctgaca aagctttcaa agaagtttac
     2221 ggttccttat gttcctgcgg ttttccagtt agcagtagct tgtattatga tgagccttgg
     2281 ttctttcgac tttttaacag acatgttgat ttttgtgatg tggctattca gtttactgat
     2341 ttttatcggt gtctttattt taagaaaaaa agcgccggaa ttgccacgtc catataaagt
     2401 accactttat ccaatcgttc cgattattgc gattctcggt gctattttca ttttaggtat
     2461 gacgatgatg acgcaaacga aattagcatt gatcggtata ggtgttacct tgataggtat
     2521 cccagtctat tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt caacaacaga
     2581 cagaaaaagt acgaactagg tcctctagat cttcgtgctt tttctatttc aataaaacaa
     2641 gttcttctat tttgattttt gctacttctg caagtagtgc ttatgtgcta aagtaagaat
     2701 gaaaaggaga gataaacatg atatttgatt ctcataccca tttaaatgct gaacagttca
     2761 atgaagatat tccagaaacg attcagcgag cacaagaaat gggggtaacg aaaatggcgg
     2821 tcgtgggctt cgatacgcca acgattgaaa agtctttgca attaagccat gactatccaa
     2881 atatatacag tattatcggc tggcatccga cagaggctgg cagttatacg aaagaaatcg
     2941 aaaataaatt acaagaacaa ttgactttac caaaggttgt agcattaggg gaaattggct
     3001 tggattatca ttggatggaa gacccgaaag aagtccaaga taaagtattt cgcagacaga
     3061 ttgctatcgc aaaagaaatg aacctaccaa tcagtattca tactagagaa gcacttgaag
     3121 acacgtatca aatcctaaaa gaagaagatg ttcgagatat cggtggaatc atgcatagtt
     3181 tcagtggtga ttatgaatgg gcaaaacgat ttcttgactt agggatgcat atttcattta
     3241 gcggtgtcgt cactttcaaa aaagcattag acgtccaaga ggctgcagca aatgtaccaa
     3301 tcgaacgatt acttgtggaa acagatgccc cttatctggc accagttcct tttagaggaa
     3361 agcgaaatga accaggctat actcgttata cagtagaaaa gattgctgaa cttcggaatc
     3421 ttccgacaga agaagtggct gcccaaacat ggacaaatgc gcaccgatta tttggtctaa
     3481 gcgaacatgg ataagatgaa gatccaagag attatagtcg ttgaaggaaa agacgataca
     3541 agaaggctgc gggaagtagt tgatgcagat acgatagaaa caatagggtc tgcaatcaat
     3601 gaagagatcc tgatgcaaat tgaacatgcc caagagactc gtggagtgat cgtttttacc
     3661 gatcctgatt tttcaggaga aaaaataaga aaaatcatta tggaagctgt tcctcaggca
     3721 aaacatgctt ttttgccgcg cacacaagct aaaccaaaga aaaagggaag ccttggtgtc
     3781 gagcatgcaa gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac gccagacatt
     3841 tcccaaacgg ctgtttctga aatcactaga caagaccttt tagactatgg tttaatagct
     3901 ggggcacaag caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat cggttacacg
     3961 aacggcaaac aattggaaaa acgtttgaag atgtttcgta ttacgccgaa agaactcgcc
     4021 caagcaatga aaatagtgga ggaacaacat gagtgaatat cgtgaaattg cgacaccctc
     4081 tagaacaaaa gagattttat tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa
     4141 ttttctgact gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca
     4201 tacgaacgtg atcgaagttg gaccaggaat cggtgcattg acggaacaat tagccaagca
     4261 tgcaaaacaa gtcgtagcat ttgagatcga tgatcgtttg atccctgtat tggctgatac
     4321 gatgcaaccc tatgacaatg tgactattgt acatcaagat attttaaaga ccgatttgtc
     4381 aacagctgtt agagaaacat ttcatgagga gcttccgtta aaagtcgtag cgaatttacc
     4441 ttactatatt acgacaccga tcatgatgca ttttcttgaa tcagatttgg ttgtggatga
     4501 actagtcgtc atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac caggaacgaa
     4561 agcttatggt tcattatcga ttgctgttca atattatatg gaagccagtt tagcattcat
     4621 cgtaccaaaa acagtatttg tccctcagcc aaatgtagat tcagcgattt tgaaactgac
     4681 aagaagagat ataccggcag tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa
     4741 agcggcattt cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa
     4801 agatgaccag acaaaagcat ggttagcaaa aagcttggaa acagctggaa tcgatccaaa
     4861 acgtcgtggg gagactttgt cattacaaga attcgccgca ttaagcaatg cgatgtcaga
     4921 aaatatgcag taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag
     4981 taataagaac agccaaacaa aaatagttcc tcatgttttt cgtttgtttg ggcttattac
     5041 cgcagatgca agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc
     5101 taagtcacaa ggaacaattt caaaaaacag cttctcatgt ttttgagtta gttggtctta
     5161 ttacttgatg caagcttttg accactatta tgccgaacaa ggagatgtga cacaaatttt
     5221 gtcacatctc ctatttttgt ttcttgatca aaaaattctt atacttgata ctcacactca
     5281 ttttgtatgg atttagaaaa gaatatgtgt cttgacagtt attagtaagt gagttatact
     5341 aagtcgacat ttatgtgtca agacatatta ggagagagaa aaaatgagaa aaaattcagt
     5401 tcgaaaacta acgatttctg ctttattgat tgggatgggg gtcattatcc caatggtcat
     5461 gcctaaaatc atgattccac cagcttcctt tactttggcg tctcatgtgc cattatttat
     5521 tgcgatgttt tttacaccag gagttgcagt ggctgttgcg ctaggtacga catttggttt
     5581 ctttttaaca acgccggtta tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt
     5641 gattggggct tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac
     5701 ttttgctaat tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc attctttagc
     5761 agaaatgcta gtagtcagca tcttttactt tattggtaat atgcctgaaa catattattc
     5821 tcaaggatat ttttatacag tattcatctt gatggggatt ggtgggctta tccacagttt
     5881 agttgattat aatattgctt attttatagc aggaacactc agcaaatatt ttgatatccc
     5941 cgtatttacg gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa
     6001 aacgacatat tagacatgat ctctcaaaaa aaaaactctc atctagtaat ggataataaa
     6061 tcctctaaga tgagagtttt ttgtcctaaa aagagctgca ccaacatctt ctggcacagc
     6121 tctaacaggg ggcaatttat ggaagtttga tgacttgttc tgtttcttta tcaaagaagt
     6181 ggcctttgct gatgttgaag gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg
     6241 catctacttt tgagatgaac tcagtagaac cagtttttgt gtaaagcatc gtttcagctc
     6301 caagtaattc ggaaacgaca acttctgatt tcactacggc ttcaggtgaa gcttcaaggg
     6361 ctacttgttc actgtggatg tcttctggac gaataccgaa gataacttct tttccttcat
     6421 aacctttttc aactaatact ttgttttttc cttcaggaat acgaagctta agcccatgtc
     6481 catctgtgat aacaccattt ttcaatgtca cattaaagaa gttcatggca ggagatccaa
     6541 taaagccggc aacgaacata ttgacgggtg tatcataaac ttctttcggt gtaccgattt
     6601 gttggataaa tccgtctttc atgataacga ttcggtcagc catagtcatt gcttctgtct
     6661 gatcgtgagt aacatagatg gtcgttgttt caagacgctg gtgaagtttg gcaatctcag
     6721 cacgcatggc tacacgtaat ttagcatcta aatttgataa tggctcatcc attaagaaga
     6781 cttttgcatc ccgaacgatt gcccgtccca atgccacacg ttggcgttgt ccaccagata
     6841 aagcagctgg ttttcgtttc aagtattctg tcagacctaa gatctctgct gcattgtcca
     6901 cacgtttttt gatgtctgct ttatcatatt tacgtaattt caagccaaaa gccatattat
     6961 caaaaacagt catatgagga tacaatgcgt agttttggaa aaccatggca atatcacggt
     7021 ctttaggtgc cacgtcattc atgacttttc cgccgatatt tagttctcct tcagagatat
     7081 cttccagtcc cgcgatcatt cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa
     7141 aaacgataaa ttcacggtct ttgattgata gattaaaatc agttacagaa taattttctg
     7201 cattgtcata ttttttatat acgtttttca gagccatttc taccataata cgttccacac
     7261 tttctttttt agttttatta actatttaaa gtataaatga aagcgttaac taacttcaat
     7321 gtcatgttga acaagaattt tttctttttt ttgtcaatgt gctagtttta agctttaata
     7381 aatatccata agaaataaaa actgatacac tggagtaaat aagtatgtta acaatatcaa
     7441 aaggagtgtc tcttatgaaa atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg
     7501 tgaaattagc aacagcgtat aaaacaatat tggaaaaaca tgaacttttt gctaccggga
     7561 cgactgggca gaggatcacg gaagcaaccg gcttgccgat ccatcggttt aaatccggac
     7621 cgttaggcgg ggatcagcaa attggagcca tgatttcgga ggatcagtta gatttggtga
     7681 tttttcttcg agacccgctg gctgcacagc cccatgaacc agatgtaaca gcacttatcc
     7741 gtttatctga tgtctatgag attccgttag ctacgaatat cggaacagct gaggtgctac
     7801 taagaggact ggaagccggc ttcctagatt ttcgaagaat cgtccatgaa atagacaagc
     7861 atccactttc attttgatgg ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat
     7921 gtagctataa aagctaaaca aactgataat atgcgatata atacgaacgt attggataat
     7981 ataaacaata atattagttt ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct
     8041 gtgggaaaag atcccaaaaa taatacagag tagaaaataa agcgttaagt gctaagagga
     8101 tgggatgttg cctcttggac gaaggacgta atgttcgcgg ttttattttc gcattcgctg
     8161 catgatacga tgtagcagct cttaaaggag atgctaaaaa tgaagaaaca ccggttagac
     8221 gcacgtatga ttgctattat gggcttattg attgcattga tggtcacttt gtcccgactg
     8281 gtagcaatcg agaccccatt tattaaaatc agtgtcacat ttattccaca agtgatcatg
     8341 ggcattcttt ttggtccttt ttggtcaggg ataggcgcag ttttagctga tttagtagga
     8401 atggccctct tctcgaaatc tgcattcttt attggattta cattgaatgc atttattgaa
     8461 ggagcaatct acggattctt tttctatcga aaagagatca cttggaaaaa tgccattctg
     8521 gctacgctta gtgtcacgct gatcatcaac ttatttttga caccattatg gcttgcgttg
     8581 atgtaccatg tgccattgtt cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta
     8641 atatggttac cgattcaatc aattgccatt tattacgtag gacgtagtat tccttacaaa
     8701 aaaattttaa gaagtttagc gatccacgct aaataa
//
LOCUS       DACEUF010000095         8604 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.099, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000095 DACEUF010000000
VERSION     DACEUF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.099"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            173..634
                     /locus_tag="IXG20_002296"
     CDS             173..634
                     /locus_tag="IXG20_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1610474.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            complement(1132..1671)
                     /locus_tag="IXG20_002297"
     CDS             complement(1132..1671)
                     /locus_tag="IXG20_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1610475.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            complement(1699..2436)
                     /locus_tag="IXG20_002298"
     CDS             complement(1699..2436)
                     /locus_tag="IXG20_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1610476.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            3082..3168
                     /locus_tag="IXG20_002299"
     CDS             3082..3168
                     /locus_tag="IXG20_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1610477.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            complement(3289..3978)
                     /locus_tag="IXG20_002300"
     CDS             complement(3289..3978)
                     /locus_tag="IXG20_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610478.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4218..5132)
                     /locus_tag="IXG20_002301"
     CDS             complement(4218..5132)
                     /locus_tag="IXG20_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1610479.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            complement(5114..5494)
                     /locus_tag="IXG20_002302"
     CDS             complement(5114..5494)
                     /locus_tag="IXG20_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1610480.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            complement(6187..6807)
                     /locus_tag="IXG20_002303"
     CDS             complement(6187..6807)
                     /locus_tag="IXG20_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1610481.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            complement(6804..7856)
                     /locus_tag="IXG20_002304"
                     /pseudo
     CDS             complement(6804..7856)
                     /locus_tag="IXG20_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7909..>8604)
                     /locus_tag="IXG20_002305"
     CDS             complement(7909..>8604)
                     /locus_tag="IXG20_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ1610482.1"
                     /translation="MDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTY
                     VWNELLQDINSRGVQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRV
                     KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPP
                     SIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGF
                     QQVTDTLVSMFTE"
BASE COUNT         2553 a         1391 c         1343 g         3317 t
ORIGIN      
        1 agacacccat taaagcagaa agacataaaa attttttcat aacttatttc tcctatcttt
       61 tttactagaa ttattttatt ttagtgtgtt ttataaaata aagaactttt ttggcgcgtt
      121 agtattttta tgataaaatt ttatgtaaat aatcgtttac ataggagaaa aaatggagga
      181 tttaggatct aagctaagag aattaagagt gaaaaaaggg tatactcaag aatatgtggc
      241 aagaaaattt aacgttacac gtcaaactat ttcaaagtgg gaaaataaca aaacaacacc
      301 agatatagag acactaaaaa aaatttgtga attttatagt actaacataa attctatttt
      361 aaatgaagat aattcaaaaa ataaaaaaaa taatttactg gtattgacag ctattatttt
      421 taatatattt gttactatat taggtttatt tgaattgatt caaatgaatt tttttagctt
      481 tacacatgga atatttactt ggtttgctct atctggatta atagtaatag atatcttaaa
      541 ttatttttat atttactatt cttatttaaa aaatgaaaaa tttttttata caatgttttt
      601 ccccctagtt attttgatta ttttattaag ctaatatgaa gtagttagtt atttaattgc
      661 atagttgaca taatgtacac tatgccaagt agcccctcta ccaatggata gagacgctca
      721 ctttgtgatt tttaattggt ctagctaaaa gcaactttct aaagcttgat ttttcgcggt
      781 tatctatatc taattctttg tgcattttta tctgtcattt ttttaagact tgttattttt
      841 tgtaaaatat ggtaaatttt tggtagcgct tcataagcgc gtgctttttc aagaataaac
      901 actcattatt ttttaactgg ctgctcggaa gagcagtctt ttttattatt tatccgtctc
      961 ttttacaaac aattcttcgt taggactgtt gtaataattt ggattcaatt tcaattcacc
     1021 atgtttattc cctttattga ttactgacaa taacatcgga ctgaagccta gaattcctag
     1081 taaaatagaa ccgaatagaa gattttttct tgtcatgaat gttccctcca gttatttcca
     1141 aagttgccac catttttgct tcttcttttt tgtgtgtttt tctatcgatt ctgtttcttc
     1201 agaaagaata gtagttgatt catcttctgg agtttcttcc gtataagcta attgtaactg
     1261 ttcttgcagt ttttcgatct gttgatttgc ctgtagggtg agctgttgtt gctggtcgag
     1321 taatttttgc atattagaat tattttgctc tttgagggaa agttgttctc gtaaaaattc
     1381 attttcttca cgcaacttat ctagcaactc gtttacaatc acgtttttgc ttgatatata
     1441 aacgttttta tcgttttctt gttcatactc atgcaaccta tatagtaacc tttggttacc
     1501 taagttgcta gcaagttgca atactccaga acgtaataca accttataac cacgctcatt
     1561 tttagctaat aaggacgggt ctaatttatc aacatgttta gagattgctt gcctacttat
     1621 ttcaaataat tcagataatt cctttattgt aaaggtttct ttgggttgca tgataattgc
     1681 ctcctaaatt gttaggtatt atttctctaa ccaattatct aaggtaattg aaggtatatt
     1741 ttcttcaaaa taaatttgca gagatcctat cttatttcct ttgtaggctt taattttttt
     1801 gatttcaaaa gttttaaaaa tagcagggtg agttgaatta acgtttaaaa attctttttt
     1861 cgcttgggca aaaatttgtt tatcaatatc tctcatgcga taactttctg gtattgctaa
     1921 tagagctcta aaatcttcta tttttacaaa ccagtgacct tttttagatc ttctgtcttt
     1981 atgagccata agttggcgat ataactcttt aacgtagcta ctctttaagt ttgtcatatt
     2041 ttctaattcg aaacgagtaa aattagttcc gattgaatta agtatatatt caaattcttc
     2101 agaaaccttt attaataatg tttcattcac tttatcgatt ttaaattttt gaaacagatt
     2161 taattgttca tatgtttttt cgtcttcata aataaaagac aaagtaatga ttttttttga
     2221 catttttatt aaatcattag caaaacggtc gccacgtgca tttatattgt aatcaattaa
     2281 ttcttttatt tgtttaaatg taaaaatttc ttcttttgta ccttttcttt tcattttaga
     2341 acatattccc caaaataaat ccatatctat aggggtaaat tttcttaaag ggacagaatt
     2401 taatccatta ttatatcgaa ctatatcgtt actcaataaa aatcacctca ataaattatg
     2461 ttatcataga cataagagga tgtaaaacac aatttatccc ccatagtgtg acaatttatc
     2521 ccccatagtg tgacaattta tcccccatag tgtgacaatt tatcccccat agtgtgacaa
     2581 tttatccccc actacctatg tcaatactta ctctcccata aggttgctaa tccctaacag
     2641 ttattattaa cagtatatct aacagtatat ctaacagtat atcttaagag cacattgaat
     2701 ttcccgccta aaaaactcct actttttcct ccaaaaaggt tcgcgaattt ttctaaactg
     2761 gaaaacttgc tccctacggt cgctaaaaag gtgtgatcgc tatgctctca tattatactg
     2821 cgcagtcgcc tccggctcct ttgctaaaac cgttgttcaa agaggaggtg tgagactctg
     2881 aaagcttgtc taagccgttc taagacaatt cttgattaaa tttcccttaa tctagtcgag
     2941 aagcgcctta caggcggtca gggcaagccc taacaaccct aaaacaagtg atacttaaaa
     3001 acaccacaca aatttaattt tttaagatat aataacttgt cgcctatccg acagtcttgc
     3061 gactggaagg aggtgtatct aatgtttgaa ctgttcattt gcccccttct tgtaggagtt
     3121 tgtgtctctt tattcagtta ttggttgaat aaccgaaaca agaagtaaaa tcaataaagg
     3181 cgacatcaac ctgaaaaaaa gccccagaga atccgacctc tctggggctt tcgtgtatct
     3241 aatgtttgat catttgcaca ttcagtttaa catgatttta aatcaaattc aattttgttg
     3301 ttgctgatct aaccattcct tgacactttt tagatagttt aaggcggttt ctttttttgt
     3361 cggattttgc atgttagttg cttgttgaag taagatttca tagcgttcat atgtgacaga
     3421 ttgttgtttt tctttttttt cttcttcact ggcaaaccaa gggtttgtgt ctccaaatag
     3481 atcactacag gcttcttcta tctttgtatc ttttacatat tgcacgtctt ttttggttcg
     3541 ctcatagttg tcttgaacct ctttttgctg tttttctggc aagttttgat aaagacgagt
     3601 gagtttttcc atgtttttat ccgtcaaacg tttttcttga ttcacaaaaa tatcgttcat
     3661 ttcctcataa caagtggtaa actcttccat tttttgtgct aatgcttgct cttttttctt
     3721 tttatcacgc gctgattcat acttttgagt gacatttcct aataaaatac ttctagaaac
     3781 gcgcttattt ccctctaaaa cgccgaataa gccgcttgaa acgattatca aggaaaatat
     3841 acagccaaga aagaaaaatc gcttaaatcg aattttagag ccttttaatg aatatcttgt
     3901 ttgaaaaatt ttatagatga gccaaatact tcctgaaata ctaacaaaaa tgagtagata
     3961 gggtaaataa aatgccattt tagcctcccc atactaacca aaatgcgaac agtgtcagga
     4021 ctaagcaaca taagggggca agaaaatcca atgatcgttg aataagcacc tttttctgtt
     4081 ccttatttcg ctggtctaat agctcttgat ggagtttttt aacttgtaaa tcaatcgtag
     4141 tttgtatcct ttcttgcgta taaggaacga gaaaagcacg ttgtttcttt atttcgtttt
     4201 ctatgtcttt taacgtttta gagcgataat tcttgttggt ggtctgttcc tctttcaacg
     4261 tgttgttcat ggtttcttcg agccggtcta atttccggtt gttcaggcgt aagagttcgt
     4321 tgttctcttc gtacttcttc tgcagtttcg cgaatgctag ctcgagctct tcgtagctcg
     4381 ttggttcgct gttggttata ggttcgttct cgtctgtcca gctcatgtaa aatcgtctcc
     4441 ttttcgtagt cctttcctag tctgttggat cgtacttttt tctttttgtg gatcaattcg
     4501 taagtgaaat ttttcccgcg attgaagcaa tgaatcccca atgttttgag ggaattacaa
     4561 aattggtcat aggttctggt ttttttgttt ttcattgttt gatcgatcgc aatccgaatt
     4621 tcgtctttcc aaacaggttc atttttttct tttaatttta tttcagacat ggttcgtttt
     4681 tctggtttct ttcctcgctc agtgattggt aagttatgtg caagacaaat ctcgtcattt
     4741 aggtcataac atttgtttag aaatgcttga ccatgagctt gaaatttttt tccagtctcc
     4801 agattaacgg agttaatgac aatgtgatta tgaatatggt ctttgtctgt atgtgtgtaa
     4861 atagctgttt ggtgatcggg ggctattttt tttgcatact ctgcccctag ttgattagct
     4921 ttttcagcgg taatttctcc tggtcgaaaa gattgaatca ataagtgtgc ctgtacacgg
     4981 tcgtcttttc catacagcat gcgaatttgt ttcatttcac ttttggcata atcgacgtca
     5041 cagtttattc ctgttttaat ggttgctcgt ggttctgcgt aattaattgc acgactacaa
     5101 gaagtggttc ggcttacctt aattgttgcc atatcttttg tagctccttt tgcatgattt
     5161 ttaggttttc ggataaacgt tgagcgtcac cagaagcaac gtcatgttgg tgtgtattgc
     5221 accacttagc aatttgattg atattccctc cagctttggc taaatcgcgc gcgattgcct
     5281 gggcaatgtc tggcgccact ttcggtgtca ccagacgcgc gttttgtgcc ttggctttca
     5341 cgaaagccgg cacactcatt tgcaaagctt cagccgattg cttcagcttt gcaaattcgg
     5401 attcgctcac tcgaaaactg atttgttttg gctcggatcg ataggtatat tttttagaag
     5461 aattgctagc caaattttga ttttgttcgc tcataagtcc caccctttca gtacgtccta
     5521 cggacgtatc gttcgcaata ccattgtacc taaaaaggta caatgagtaa agctcactaa
     5581 aagggtttgg caagctctat atttgctagc caaataccta cgtatttagt gcaaataacc
     5641 gcttgtcaaa ggggcgcaag ctcccccttg aatccccctc aaaagccacg ctccacaata
     5701 caaaaaaagc tagcaaatgc tagccacttt ttgtaaagtt tcgcttattt caccgaagat
     5761 gaaattgtta ctttgcaaca aaaaacataa atgctttttt gtgtaaacta ctaactattt
     5821 agctaaaact gatagtttta ttaaacattc agcaatattt ttagccaatt ttttatcaga
     5881 ctttcggcga gtcattatga taatatgtcc actttttgtt acttctacca aaaatctagc
     5941 aataactctc ccattcttca gttgatttag tttcataagt cttttgtaat tgacggttta
     6001 atttttttgg caattgcaaa gaataaataa gtctatcata aaatttttca tactcttttt
     6061 tcaataaaat cactagggtg cctccttttt attaagtgat tatttaaatt ttgggatagg
     6121 agagatggaa tataaaataa atacccgtgg tgctagtttt aaatcagcta gcatcacggg
     6181 taaaaattat aattctataa tattagttcc atagtttaga acatttaagt catgtgttac
     6241 tagtacaacg gctttttttt ctttttctgt taaatttttc agaaagttca ttataaaagt
     6301 tgagttttct ttatctaaat ttcctgtagg ttcatctgca aataatatat caaatggttt
     6361 taacatattt ctaattaatg ctattctttg ttgttctcca ccgcttaaga gaaagatttt
     6421 ttcttttaag atatcttttg gcaaagaaaa atctgttaat aaattataaa tatgatcatt
     6481 ggaaatatta ctgttaaact tttttgctaa ttcgaaattg tattgtactg tttgattttc
     6541 aagaagagca tagttttgaa ataaatagct aatatgattt cttcgtagac gttgtatatc
     6601 atgtttagaa actttctttt caaaatagaa taagttaccg gtatattgtt tatctattag
     6661 gcttaaatta tttaacagag tagtttttcc actaccactt tttcctgtta ctataataaa
     6721 atctttttct gatattgaaa agttccaatt ttgtttgatc ttttttttat taattgacag
     6781 ggtgaaattc tcaactgata atatcataaa atattctcct taaaatattt aaaactattt
     6841 tttttataaa taataataga agtaattaga tttattaaaa agatcataaa aattataaaa
     6901 tacaagtaag tgttttttga aaatatcaat tgaaaacaca tagtagcaaa taactctatt
     6961 gtaaaaggga gtaaacataa atctaaaact tgacttattg gtgaatatcc aaatattttt
     7021 tttaaagcta ttttttttga atttatagta aaactaacaa aaatcatttg aaaattaatt
     7081 attataaata atcctaatat taaaagatat tgtagatatg catttaattt atttactttt
     7141 ttttgagata ataattcacc agtttcttga aaaacagaat aagaaaaatt tatgatattt
     7201 tctaggttaa attcttttat atacggatta atttgattaa aaaagttttg gtttgaagta
     7261 tgtttctgat caaaaaataa tccaccatca ctagtcaata tatttcccca aaataatgat
     7321 tctgttattt ctggattaat aactctaact atgggatcag ttattgtata gttatctttt
     7381 gcctttccat ataaggtatt aaaactaaaa tacttttggt tattattaac aaatattagt
     7441 tttattttta aatttttcaa attaggattt ttataaggag cttgaggagg agagtcctca
     7501 agtctaaact gtatgtcttc taaataattt ttttcaatca tttttgcata ttctttatat
     7561 tttttaggta ctagaatgta agtagttagt ggatcagagt ttattaaatt taagtcagta
     7621 gattcttcaa aagaaatagg attgtaatat tttagatagt ttaaatcaac acatattcct
     7681 gcaccagcag gttgactaac atagtcttca aaatttggat aaactttttg tccaataaaa
     7741 aattctttat tattctcttg gatagttgcg taattgtatg gcgcgataat aaaagtttta
     7801 taattcttct gtattttata aacaaattta ctagcttttt tcaagtagtt attttcggga
     7861 gcgtcaataa ttttgtgtaa ataaattgtc ctcctgcaaa ataattagtt actcagtaaa
     7921 cattgaaact aatgtatcgg ttacctgttg aaaaccttta tggcttctgt ttagaaattt
     7981 ttgattgtat gtatcaaaaa tgctgactag aaagcgttct agtgattctt cattttgaaa
     8041 ctgctctttt ctacggctgt atcttttaat ttgcttattg aaagactcga ttagattggt
     8101 tgagtaaatg gttctacgaa tgctaggtgg aaaatcataa aaagttaata agtcttggtt
     8161 ttctatgagt gactgcgtca ctttaggata gtttttcttc catttctcaa tcatgccgga
     8221 taagaaggta ttcgcttctt cttttgagtt agcttgataa acagccttaa agtcatcaca
     8281 gatttctttt cggtctttga cacgtacttt atgagcgatg ttacgagata catggataca
     8341 acaatgctga tattttgctt taggataaat ttgatggata gtatctttca tgccttttaa
     8401 gccgtccgta ataaaaagca agacttcttg aactcctctg gagttaatat cctgtagcag
     8461 ctcattccaa acgtatgttg attcagttgg agcaatcgca taactcagta cttctttagt
     8521 gccgtcttct cgtataccaa tggcaatata aatcgcttct ttggatacgg tttgacgttt
     8581 tagtggaatg taagtagcgt ccat
//
LOCUS       DACEUF010000096         8287 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.100, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000096 DACEUF010000000
VERSION     DACEUF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.100"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            216..1409
                     /locus_tag="IXG20_002306"
     CDS             216..1409
                     /locus_tag="IXG20_002306"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ1610483.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1539..3011
                     /locus_tag="IXG20_002307"
     CDS             1539..3011
                     /locus_tag="IXG20_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1610484.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3094..3882
                     /locus_tag="IXG20_002308"
     CDS             3094..3882
                     /locus_tag="IXG20_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ1610485.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3949..4617)
                     /locus_tag="IXG20_002309"
     CDS             complement(3949..4617)
                     /locus_tag="IXG20_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1610486.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4732..6444
                     /locus_tag="IXG20_002310"
     CDS             4732..6444
                     /locus_tag="IXG20_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1610487.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6447..8219
                     /locus_tag="IXG20_002311"
     CDS             6447..8219
                     /locus_tag="IXG20_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1610488.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2538 a         1387 c         1821 g         2541 t
ORIGIN      
        1 ttaaaaagct tagcagagtt atctttatgg atgattgtgc taagcttttt tggacagttc
       61 taatgtaaaa tatttttata tttttaagca aaaaaatgaa aaaatacgag cagaaaactg
      121 ttttatctgc tatacttggt tcgttgcaag ttcccgaaag gatccgcagg acggagatgc
      181 cttgtaaccg aatttaacta gggggaatca ttataatggt agaaagacac ttatttacat
      241 cagaatctgt ttctgaagga catccagata aaattgctga ccaaatcagt gatgcaatct
      301 tggatgcaat tttaaaacaa gatccaacag cacgagtagc gtgtgaaacg tctgtaacaa
      361 ctggtcttgt tttagtattt ggagaaatat ctacgacggc atatgtagat attcaaaaag
      421 tcgtacggga aacaataaaa gaaattggtt atacacgtgc aaaattcgga tttgatggag
      481 atacagcagc tgtattggtt gcgattgatg aacaatctcc tgatattgct caaggagttg
      541 atgaagcact tgagatccgg gatgaagata aaaaagacgt attagatgag attggtgctg
      601 gtgaccaagg tttaatgttt gggtttgctg tagatgaaac accagaattg atgcctttac
      661 caatcgcttt gagtcatcgt ctggtacgac gcttggcaga tttgcgtaaa tcaaatgaat
      721 taacgtattt acgtccagat gcaaaatctc aagtaacggt tgaatatgat gatcaaggac
      781 aaccggaacg cgtagataca atcgttattt caacacagca tgatgatgca gtagataatg
      841 aaacaattcg tcatgatgtc atcgaaaaag tagtgaagga agttattcca gctgaattat
      901 tagatgatca aacgaaatat tatatcaatc cgactggccg atttgtcatt ggtggtcctc
      961 aaggggatgc cggattaaca ggaagaaaaa tcattgttga tacgtatggc ggttatgctc
     1021 gtcatggtgg cggtgctttt tctggtaaag atgcgacaaa agttgaccgt tctgccagct
     1081 atgctgcacg ttatattgct aaaaacattg ttgcagcagg gcttgctcgc aaagcagaag
     1141 tacagctagc ttatgcgatt ggtgttgctc aacctgtttc gatctcaatc aatactttcg
     1201 gtactggaac ggttccggaa gaagaactga ttgctgcagt gagagaaaac ttcgatcttc
     1261 ggccagcagg aatcatcgag atgcttgatt tgcgtcgtcc gatttacaaa caaacagctg
     1321 cttacggtca ttttggtcgt acagatgtag atttgccttg ggaacatacg gacaaagtag
     1381 atgcgttgaa agcaagttta gcaaaataag ttatattcag ctggaacgat cgccaagtgg
     1441 tggttgttcc agcttttttt atggcagtta acctcttgaa aaagatagta attatttatc
     1501 tatttttaga tcctgtcata atgaaaggga ggaataagat gaaaaaagaa acaaatgtta
     1561 ctttggtcac agtgagtatt ttcattgcaa cgttcatgac ggctattgaa ggtacaatcg
     1621 taacaaccgc tatgccaacg attgtcggtt cattacatgg aatggaaatc atgaactggg
     1681 tattttcgat ttatttgctg acaaacgcta tgcttactcc gatttacgga aaacttgcgg
     1741 ataaagtggg acggaaacca atctttattt ttgggacatt attgtttatt atcggttcgt
     1801 tattttgcgg tttatctcaa agcatgattg ggttgattgc tgctcgtgcg attcaaggga
     1861 tgggtgctgg ggcaatgatg ccagtagctt tgacgatcat cggcgatctt tattctattg
     1921 aaaagcgagc aaaagtttta ggtttgaata gttcagcctg ggggattgcc agtgtttttg
     1981 gaccgcttgc gggaggattc atcgttgaaa cgatcagctg gcactggatc ttttttatca
     2041 atgtgccgat cggaattcta ttaattattt tgatttggct atatttaaat gaaccaaaaa
     2101 tcatacatga gtctaaacca atggatattt ttggtagttt tagtttgatg gccatgcttg
     2161 tctcattgct gttagggttc caattattag gagacgaagg aatgagtccg cttgtttctg
     2221 ctttgctgct aggtagtatc gtaatgttag gtatttttgt ttttgccgaa aaaagagcta
     2281 aagatcctgt catcatgatg catttattca aaaatccact atttgttgtg gtcaacttgg
     2341 ctgcagcatt ggctagtggt tttttgatgg gaatcgatgt ttatatccct atgtggatgc
     2401 aaggtgtatt agggctgaat gcagcaattg gtgggctggt attagcacca ttatcactag
     2461 tctggatggt cggttctttc cttgcaagca agtggatgac tcaaatgaaa actcattggg
     2521 tcttgagaat tggactacta atcgcagcta ttgggggcgc ttggttgtat cttatgcctt
     2581 tcagaagtaa ctatttatgg ttttttgcta tatcaacggt catcggtata ggcttaggtg
     2641 tgaccatgac tacctcgact gtcagtgcac aaaatagtgt atctcgtgac cagcttggtg
     2701 tagcaacttc ttttaatacg ttagttcgaa caattggtca aacagtcatg gtttctattt
     2761 ttggtttatt gataaatggg gtaactaaac gagaacttgc agcagcaaat ttaacaaatg
     2821 atgcagatat catgaataaa ctggtgaatc cacaaacggc taaatatatt gatgcggata
     2881 ttttgttacc attaagaaaa attctttatg atgggttaca ttccgtttat ctagttggct
     2941 tgttactcgt gatcattgct ttggtcttaa cgcttttcgt tcatgaaaaa agtggtaaaa
     3001 taaaacagta gtttcggtta agatatgtac gtagccgaat cattcaaatt taaagttatt
     3061 caaaaataac ttcttactaa ggaaggcgaa aatatggcat ttattcaagc aaatatttat
     3121 tcgaatgtgt tagaaatgga agtcaatcta aatgtgatac taccgcaaac aacaaaaaag
     3181 gtgattggca ccagttcaga gcaagagaca acggatatac ctgttttata tttacttcat
     3241 ggaatgggtg gcaatcatag tgtatgggaa agacgtacgt cgattgaacg ttatgtagca
     3301 aactacggat tagctgtcat catgccatct actgatcttg gctggtacac agatacagtt
     3361 tatgacatga agtattggac attcattgca gaagagctac ctgagatttg tcatgaatta
     3421 ttcccgcaat taacaagaaa aagagaaaaa acatatgccg ctggattatc gatgggcgga
     3481 tatggcgcgt taaaactagg ccttgcaaaa tcagaaaatt tcgcagcagt aatctctctt
     3541 tcaggcgctg tttctgtagg aaatcgaatg gatgatcttt tgatggttcg taaagcatct
     3601 ttttgggaag ggatctttgg accattggat caaatcgaag gttcaaaaaa tgatccgcaa
     3661 tacttattga aagaactagt aaaaagcgga aaaccagttc caagatttta tatggcttgt
     3721 ggggaatctg attttcttta tcaggcaaat caggaaatgg tatctgctat gcaggaacaa
     3781 ggcttggcgg tcacctttga acaaggacct ggcgatcata actggatctt ctgggaccaa
     3841 tggattcaac gtgcattggc gtggctaatg gaagaaaaat aaaaaaagag tcttaaaaag
     3901 atagctccta tagagatcac aaacatagtg atctctatag gagctttttt actgctcgtt
     3961 ttctcttctt ttattttgtt ggcataagcc ttcaatatag gtttgtgcac tcgttagaaa
     4021 gagtgctttt tcattcaatt ctgcatggga ctgcatactt tctttaaatc cctctaattg
     4081 attttcaact acataattac gcataaatag aactgtttct ttcaaaaatt catttccgtc
     4141 cataattttt tgtcgaagct gctgttcatg ctgctgatcc attaactgtc caatcaataa
     4201 attgtggaga gaggcaatgg cctgatgact ttgggtgact gaaaatcctt cacgaacaag
     4261 taattggatc atttgctcca aatgttctaa tcgactcgga aaagcgggca cagtattgat
     4321 ttctaattct gccccacaag ggaaacgtgt ataaaggtca tagtaatttt ccatatactg
     4381 cagcaactgt tcattccaag gcaaagttgg atctggaagc gctaattctt cttccattgt
     4441 ttcagccatt ttttgcaaga gctgttgttt gtttttgaaa taccaatata aagcaggtgc
     4501 ttgaacatga attctgtttg cgatgtttcg catggaaagc tgcgaaagag cttgtgtttc
     4561 agccaggata tcaaaggcgg cctgaatgat gattgcttga gaaagcttta tttccatttt
     4621 atcaacctcg tgtattgcat tatcttttta aacatggtaa attgttagtc ggtctaattt
     4681 tacagtgtta aattagtcct gtcaatttaa acaaaggggt gtaatcaacg aatgaaatta
     4741 atcttacgac atgcgaaaaa atataaagta gctgttttta tttccttgct gtccgtagca
     4801 gtcatggttg cagcagcatt atggcaacct aagttactac agcaagtatt ggaagctatt
     4861 attacagaag ataatgatga aatgaaaaca attggtattt acctgatcag tctggctttg
     4921 ctaggattag ccgcaggtgt aacgaacacg attttctctg caaaagtagc tcaaggcgta
     4981 agtgcagata tccgagaaga agcatttcga aagatccaga cattttcatt tggaaatatc
     5041 gaacagttct ctgctggaaa tcttgttgtt cgtctaacaa atgatatcac gcaaatccaa
     5101 aatttagtga tgatttcttt gcaatcactt tttcgaattc cattcttatt tattggtgcc
     5161 ttcattttag ctatgatcac gatgccgcaa ttatggtgga tcatcgtagc gcttatcgtt
     5221 acggtatttc tgattacggc actttcattt acaaggatgg gcaagcactt catgatcatt
     5281 caaaaactga ttgatcgtgt gaatagcatt gcaaaagaaa acttactagg gattcgtgta
     5341 gtgaaatctt ttgtacaaga agaaaaccaa ttgaagtcat tcagcaaagt aagtgaggac
     5401 ttaacaaaac acaacatcat agtcggtaca ttattttctg tgatgatccc gtccttcatg
     5461 ctggctgcca atttggctgt ggttggggcg atcttttttg tgagcgatct tgttaaagat
     5521 gatcctacgt taattggtgg aattgcttca ttcatgaact acttgatgca gattatgatg
     5581 gcaatcatta ttggcggaat gatgatgatg atgacttcaa gagcagccgt atcgctgaag
     5641 cggattgcgg aaattttaga tgctgaacca gatcttactt atttagacgt tccagaacaa
     5701 gagttgacgg gttctgtcac ttttgaccat gtttcttttc gttatcctgg tgaggataca
     5761 gatacgctta aagatatcag cttttctatc aaacctggag aaatggttgg tatcgtcggg
     5821 gcgactggtg ccgggaagtc aacactagca caattgatcc ctcgtctatt tgatccgaca
     5881 gaaggcaaag ttgaagtagg tggtgtagat cttcgtcaag taaatgaaaa gagtttaaga
     5941 caaacggttt cctttgtttt gcaaaaagca attttattct caggaacgat tgctcaaaac
     6001 ttgcgccaag ggaaaaagaa tgcaacagaa aaagagatgg aacatgcttc ttctattgct
     6061 caagcaaaag aattcatcga aaagttatct gatcgttacg aagcaccaat agaagaacgt
     6121 agcagtaact tttctggtgg tcaaaagcaa cgtttatcca ttactcgtgg cgttatcggt
     6181 aatccaaaga tcttgatcct agatgacagt acgagtgcac tggatgctcg ctctgaaaag
     6241 ctggtccgag aagcactcga tcgagatttg aagggaacaa cgacgattgt catcgcacaa
     6301 aaaattgctt ctgttgtcaa agcagaccgc atcttagtat tggatgaagg cagattagtc
     6361 ggcgaaggaa cccatgagga attggtagct ggaaaccgtg tctatcagga aatctttgaa
     6421 actcaaaaag gaacggagga gtaagtatga ctgatctaat aaaagcaagt aaatttttct
     6481 atcactattt gaagcggtac aaactctcct ttttctttat ctttatcaca gtgatcattg
     6541 ctacctatct acaagtaaag gcacctcaat acatcgggga agcttttcaa gaattagcta
     6601 attacgtagg aggcttgatg caaggcgtag acgataaaag caaatttgtt tctgtaatat
     6661 ggaaactctt gctgttttat gtactagcta gtgcagccaa ctttatctat agtattttgt
     6721 tcacacaagt agtagggaag tcaacgaacc gaatgcgtat cggtctattc aacaaattgg
     6781 aaaaattgac gatccgtttc tttgattctc atcaagacgg tgaaatttta agtcgtttta
     6841 ccagtgactt agataatatt caaaatagtt tgaaccaagc tttgcttcaa gtcatcacga
     6901 atgctgtttt acttgtcggg atcttgatca tgatgttccg ccaaaatgta gagttagctt
     6961 gggctacaat tgcttccact ccagtagccg ttttgatagc tgtcttgatt atccgcaaag
     7021 cacggaaata tgtggatctt cagcaagatg aagtggggaa actaaatggt tatatggatg
     7081 aaaaaatcag cggacaacgt gtcatcatca caaatggctt acaagaagaa acgattgaag
     7141 gattcttaga gcataatcaa agtgttcgcg aagcaacctt caaaggacaa gcttattctg
     7201 gtctactatt tccgatgatg caaggaatgt cactggtcaa tacagcgata gttatctttt
     7261 tcggcggctg gttagcatta aatggtgatt tggaacgttc ggtggcatta ggcttagttg
     7321 tgacattcgt ccagtattca caacaatatt accaaccact gatgcagatt tcatctggat
     7381 acagcatgat ccaattagcg attacagggg caagacgatt gaatgaaata tttgatgaaa
     7441 aagatgaagt caaaccagaa aacggtaaag catttgatgg tgttgaaaaa ggtcttgcgt
     7501 tgcagcacgt agattttggt tattctccag aaaaactagt cttgaaagat gtctcgatca
     7561 atgtggacaa aggagaaatg gttgctcttg ttggtccaac aggatcgggg aaaacgacga
     7621 tcatgaatct gctaaaccgt ttttacgatg tcaatgatgg ttcagtagtc atcgatggaa
     7681 tagatatccg agattatgat cttgatgcat tacgtagaca tgtaggaatc gttttgcaag
     7741 aatctgtatt gttctccgga acgatcaaag acaatatcac attcggcaaa ccggacgctt
     7801 cagatgaaga agtcatcaat gcagcaaaac aagcgaatat tcatgacttc atcatgacgt
     7861 tagaaaaagg ttatgataca gagatatccg aagaaaataa cttattcagt acaggtcaaa
     7921 agcagttgat cagtattgct cgaacgatca tcacaaatcc atctttattg attttggatg
     7981 aagcaacaag taatgtagac acggtaacag aagcaaggat ccaaaaagca atggatgaag
     8041 caatcaaagg aagaaccagc tttgtgattg ctcaccggct gaaaacaatc ttgaatgcag
     8101 atagaatcgt tgtcttgcgt gatggggaag tcattgaaga aggcagtcat catgagttat
     8161 tagcagaaaa tggattttat gcggaacttt atcataatca atttgtattt gaaagttaaa
     8221 ttaaaagaac ctcgttataa cggggttctt tttatatatt ttatgttgtt tgtttttata
     8281 aggggca
//
LOCUS       DACEUF010000097         7760 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.101, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000097 DACEUF010000000
VERSION     DACEUF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.101"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="IXG20_002312"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="IXG20_002312"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ1610489.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3273)
                     /locus_tag="IXG20_002313"
     CDS             complement(2032..3273)
                     /locus_tag="IXG20_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1610490.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="IXG20_002314"
     CDS             complement(3291..4214)
                     /locus_tag="IXG20_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1610491.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="IXG20_002315"
     CDS             complement(4435..6927)
                     /locus_tag="IXG20_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1610492.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="IXG20_002316"
     CDS             complement(6934..7401)
                     /locus_tag="IXG20_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ1610493.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="IXG20_002317"
     tRNA            complement(7537..7610)
                     /locus_tag="IXG20_002317"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="IXG20_002318"
     tRNA            complement(7668..7741)
                     /locus_tag="IXG20_002318"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2161 a         1691 c         1247 g         2661 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DACEUF010000098         7673 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.102, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000098 DACEUF010000000
VERSION     DACEUF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.102"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(56..1444)
                     /gene="ltrA"
                     /locus_tag="IXG20_002319"
     CDS             complement(56..1444)
                     /gene="ltrA"
                     /locus_tag="IXG20_002319"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1610494.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(1897..2649)
                     /locus_tag="IXG20_002320"
     CDS             complement(1897..2649)
                     /locus_tag="IXG20_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1610495.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(3013..5679)
                     /locus_tag="IXG20_002321"
     CDS             complement(3013..5679)
                     /locus_tag="IXG20_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1610496.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6020..7471
                     /gene="cls"
                     /locus_tag="IXG20_002322"
     CDS             6020..7471
                     /gene="cls"
                     /locus_tag="IXG20_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1610497.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2094 a         1679 c         1364 g         2536 t
ORIGIN      
        1 aactgacctt ccgcccctct cacaccaccg tacgtacgga cccgtatacg gcggttcaat
       61 aagttgagtg tacgaactgg tacttcaagg agatgtcctc atatccttgg cgggccagtt
      121 ctttgtctgt taatgatcga tgaagaatat gacttttgga gactgaccaa ataccttttc
      181 ttgtatttga ccattcatac gctttcgctt tatcgatccc taaacgaatc agattttttc
      241 ttttggttct tggattcttc cattgtttcc aaatatattg tctgattctt cgcttcaacc
      301 aaccgtttaa ctccctcatg aattctttca tctcaccaat tccatagtag ttgatccaac
      361 cagtcattag ttgcttgatt tcttttaaaa taagttggat acttcttcca cggtttcttt
      421 tagtgagttg tttgagcttg cttttcacac gctgttttgc ttttgcgtga gggcgaattt
      481 taataccttt ctttgttggc agaatgcaga atccaagaaa ctttcgtttc aatggacttc
      541 ctaccgcact cttttctcga tttaccgtca actttagttg ttcttcgaga aattgaatgg
      601 atttcttcat cacacgctgt cctgctcgtt tgcttctaac atagatattg cagtcatcgg
      661 cgtatcgaac aaatttatgt cctagatttt ctagcacttg atcaaattcg ttcaggtaga
      721 tgttacttaa caggggagag agattgcctc cttggggcgt tccttcttca gttgttgtga
      781 tgagtccatt ttccattaca ccactcatta agtattgacg gatcagtttt aggacttgtt
      841 tgtcctttac tttccgctca atgaaataca tcagcttatc gtgatttacg gtgtcaaagt
      901 aggctttcat atcaatgtcc accacatatc ggtaaccttg ttcataataa gattttgctt
      961 tcttcactgc gtcatgtgcg ctgcgatttg ggcgaaaccc ataactatta tctgagaatt
     1021 ctggttcaaa tagcggttgt aacacttgat tgattgcctg ttgaagcata cgatcaataa
     1081 ccgtgggaat tcctagtttt cttacaccgc cgtttggttt cggtatttct actcgcaaaa
     1141 caggctgagg tttatagttc ccttggcgta aattggatag taattcctcg cggtgctctt
     1201 tcaagtaagg aagcagttga tcgacggtca tcccatctgc accagccgcc cctttatttc
     1261 tgactacttg aagatacgct tgattgagat tattccgatc tagaattctc ccaaacagat
     1321 tttctacacc atcttgtttc ctctgttcac cgcaagaagt gctgcgcact tcatcatacc
     1381 cttcgtgttc cacactagct ctttgatgat agccaagttt tcttgttttc tgcggtcgtc
     1441 gcatcttctc acacctccta aattattcaa gattcttatt gttcagtcct tcgtaacggt
     1501 cagtgtcgtt actactatga cctcggctga cttctcatga ttcaccatca tgtcgccatg
     1561 atcgttgtgc tggtttgttt tcattcaacg cccacaacac cctcatgaga tctcccccgg
     1621 taagagtgaa aactttcctc ccatgtaact gctagattta ctgtataggt ttcgggcagt
     1681 attggacttt gtcttgtgat gaagacttat ccgacctaat tcagccttgt atctagtttc
     1741 tgttcgtcag ttcaggagtt tgcgtccgac ttccttcagc cacaacctca cgattgccgc
     1801 cttgtctttc gctaacagtt cctactgcca agcctgtagt ggactttcac caccaagttt
     1861 tcacccatgc agggcgcaca tcataaaaag aacccttcac gagtcgtgaa aggttctttt
     1921 tttatctgcg gaccaaagga gcagtctata ggtcagctta gtcgtactaa ggatttctcg
     1981 tttttcactg aatagtttgc gaatgtacct ggtcacaaca ggatcttttg cgtacgcgtc
     2041 atagtatagc ttcatcaatt cttcagtaat tgattcctgt gtaaaataag taaaatcttt
     2101 tttcttcact ttgaatcctt catcaattaa gtatgtttca aattgatcca ttacaactgg
     2161 cgatgtatta ggatcttctt ctatcattcc tagttgcttt tcgataggat aaaaaacatc
     2221 atcgattgat tccactactc tgattatcac gcaccatttg cttgactttt tcatcaattt
     2281 ttctaattga ataagatgta ccaatgctgg atttgctgct gtaaaaacca tttcatatgt
     2341 ctcatttgat tggataaatt cttctaacga ctgtttgata aaggaaacat ttggcaactc
     2401 atttttttct gcttcttttt ttgcattttc tagcattcct gaagaaaaat cgatcaaatc
     2461 atagcgatta atttcttttg ctaaggaaag aaatctgcca gatcctccag caacgtctgc
     2521 tgctgtttca gcaggcagga ctttctccgt tttcagataa ttttttacat cttcagcaat
     2581 agttacttgc gattcttgct ggatttcata atattcttca gcaaattcat cccattcttt
     2641 ttcattcatt tgatttcctc taattttttg ttatatgttt tcacttttcg gaataaatca
     2701 ttctcctctt ttatctattc tatctctttt gatgaataat aaaatgattg gctttccgtc
     2761 tatcataaat aaaaaaaggg actgtgacca aagtcgtgtc aaagtccctc tttccgaata
     2821 aacagtgtcc aatcagatga ccctcagtat tagctcaaaa tgctgatttt acaacttctt
     2881 ccttaaacgg tgttctcctg ccaactcttc ggtcacaagt cacaatattc aacaaacatg
     2941 agaagctgtt tgttgaaatt gcaccttgtg actcagaatt aaacggcagg ctcacaacct
     3001 cgttgattcg ttttatttat ttccttgtac tcgtacaatg atcaatttag tgatttccat
     3061 caaaacaatt gtcacaagtg cgattcccat acatgtaaag acttctgtca atccaaatgc
     3121 tgatgggata gagaagactt ctttcagtcc tgggatcatt gttaagcagt aaagcgcgct
     3181 acatacaact acagctagta agacacttct gttagagaag aaccctgctt ggatagctgt
     3241 ttgtgtgttt gaacgtgctg ggaatgtttg caatgtccga caccaaatca atgttgaaaa
     3301 tgacatggct acaccgaatt caggtgaatg ctgcatgcca agccattgag caaagataac
     3361 agcaatccca atcagcacac cacgatacgt tacagaaatc aatgttttgt ccgtaaagat
     3421 tcctgaattt ggatctcttg gcgggcggtt catcgtagca ggttcagctt tttctgtacc
     3481 taatgcaatt gctggcagtg aatcattgac taaattgatg aacaacagtt gtaatgctgt
     3541 aaatggattg gaccagtcag caactaaggc aaagatgatc gcaatgatcg cacctaaatt
     3601 tcctgcaaat agataagaaa tagctttctt gatattatca aacacattac gtcctacttc
     3661 tactgcgctg acaatagaag caaagttatc atccgtcaat accatcgctg ctgcatcttt
     3721 tgccacatct gtacccgttc ccatagcaat tccgatgtct gcttgtttca atgctggtgc
     3781 gtcatttaca ccatcacctg tcatagctga gatatgacct ttattttgcc aagcacgaac
     3841 gatacgaatt ttattctcag gtgaaacacg tgcataaaca gtgacttttt ctaaaacttc
     3901 gtttagttga tcttctgtca attcatctag ttccgtcccg gtcaaagcca ggtttccttc
     3961 ttcaaaaatc ccgatttgtt ttgcgatagc aacagctgtt gttttgtggt caccagtaat
     4021 catgattgtt ttgattcctg cacttcttgc ttctgttacc gcctgagtca cttcagcacg
     4081 tggaggatcg atcatagcaa gcaaaccaac aaggatcaaa tcgtcttcat cttcaaatga
     4141 taaatcaaaa ttatcaagtg gacggtaagc aaaagctagt acacgtaatg cgcgtgttga
     4201 aaaagcttca ttttgctctt ggaatttttt catcaattca tccgtcatcg gaacgacttc
     4261 accatttagc aatacttttt tactgcgttg gaagacgata tctggtccgc ctttaacaaa
     4321 caagtaattt ttgttatcaa ttgtgtgtcc agtagacatt aattttcgat ctgagtcaaa
     4381 aggaagttct gcttgtcttg ggtagttgtt tcggatttcc tgataaggct ggtttaattt
     4441 ttcactaaag tcgataaacg ctacttccgt agggtcgccc aatttagtac cgtcttcact
     4501 gatcgatgca tcattcgcta aaacactgat ttcgatcaag cgacgttcgt cagctgtcca
     4561 ttttgaaggg tcatttgtga aatccccagt atcgccatta cctaaaaagt agtcaacaac
     4621 tgtcattttg ttttgtgtca atgtaccagt cttatctgta cagatgatac ttgtcgaacc
     4681 aagtgtttca actgctggaa gcttccgaat aatggcatga cgttttgcca ttttattcgt
     4741 tcccaaagat aacacgattg ttacgataga ctgtaatgct tctggaatag cagctacggc
     4801 tacggctacg gcaaacatca ctgcgctgac gatatctgca gtcatatctc ctgtaccttc
     4861 tccaagataa atacgaaaca gttggatacc taaaatcaat aaagacaatg ccaaaatagc
     4921 caaacttagt tttttactga atttatccag acctcgttgt aacggagtga cttgctcagt
     4981 tgttgattcc aaaaggccag caacttgtcc gatctccgta ttattcccag ttgaagtcac
     5041 taagaattta ccgcgtccgt aagttacgat cgttccactg aataccatat ttgtacgatc
     5101 tccgattggt accgtacttg tgatatccgt tacttctttt tctgcagaag tcgactctcc
     5161 tgtcagcata ccttcatcta cttttaatga tcccgcatct agtaaacgtc catcagcagg
     5221 tacataatcg cctgcgtcta acaacacgat atctcctacc acaagttcgt ttgccggaat
     5281 cgttttttcc ttaccatcac gaatcactcg agcatcgggc gctgaaaggt ttttcaacgc
     5341 ttctaaagaa ccttctgctt tttttgtttg gacaacactg attatagaat tcaacatcaa
     5401 tactgcaaaa ataactaaga attcgatgta gtcacccatg accatctgaa tcagtgcgac
     5461 aatcagcaat acgataacca ttgcatcttt gaaagtttct aagaacaatt tccaagttgg
     5521 cttcttcggc ttttctttta gctggttcaa gccatctctt tctaattttt ccttcgcttg
     5581 cgtggtgctc aatccatcgg ttgagctctg cgtttgctgc atcaacgagt cgatcgtttc
     5641 tttatagacc gcttgttgtg cttgttcctt gttttccaaa tttcatcccc cattttttga
     5701 tcttggccct agtcctgtct gaaaaaaaag agacctaaga caaatacgca tttgtcttag
     5761 gtctcaccat ttcatacaat accagaaaac catttggtta acttactgtt gatattatcg
     5821 caacaaacgt tgccggttac tcccctaagg agatattcaa ctgtactaat atcctacctt
     5881 attaacctac aaaaagcaac tactttagcc cgatttacct acattttcat gaaaggagtg
     5941 aaaagtatat tcaccttggt aggcttcttt cttctatcat gctatactaa tgttattgta
     6001 tgtaaggagg aacatttttg tggtatctag tattataacc gccctttatc tattaaatgc
     6061 acttattgct ttggtggcta ttttgatcaa accccgagat gtagcagcca tttgggcatg
     6121 gctcttagta ttgtttgccc ttcctggggt gggctttgta ttatatttat ttttcggacg
     6181 tggattaacg gataagaaaa aattctatct ccgacaaagt gacttgaaag aattagaaaa
     6241 ctttcagtct tttagaggag ataccattga acattacgat cctgacatgg gcgataaaga
     6301 caaacagcaa tttgttgact tcttctcttc attaaatcgt atgccgctga caagaatgaa
     6361 ttctgtcact cttctcacag acggacaaga gaaattggat tcactgcttc aagatctaaa
     6421 aaaagccaaa cattcgatcc atatcgaata ttacgcattt gtgacagata atatcggcca
     6481 gcaagtctta catgttttag aagaaaaagc cgcagaaggc gtggaagttc gaatattata
     6541 tgatgcattt ggctctcatg gcacaaaagc aaaagatttc aatcgtctaa tcaaaaatgg
     6601 tggacatgtc catacatttg ttacctcaca aagggcatta cttcgtttcc gattgaatta
     6661 ccatgatcac cgaaaaatcg ttgtgatcga tggaaagatt agttataccg gtggtttcaa
     6721 tattgccaat caatatgtaa atacaacaaa aaaattcggc tattggcgcg atacgcatat
     6781 acggattttc ggtgccgctt cttctttgct ccagcttcgc ttcttaacag actggaacgt
     6841 ctcggtacct gaagaaaaaa aggtcggcta tcatttgaat tatttcttta aaaaagcaga
     6901 tcgagatgaa tctaagcttg ctgatacatc catccagctt gtttcaagcg gaccgaataa
     6961 cgaaagggaa caaatcaagc tttcatttat caaattgatt acttctgcta aaaaacgtgt
     7021 ttggatacag acaccttacc ttgttcctga tgaaagtgtc attgctgctt taaaaatcgc
     7081 aactgcctct ggtgtagatg tgaaaatcat gattcccaac aaaccggatc atccttttat
     7141 ttatcgagca acacaatatt atgctcggca gctgatcaag gaaaatgtac aaatccttgt
     7201 ctatgagaac ggcttcctcc atgcaaaaac attgataatg gatgatgaaa tctgcatggt
     7261 aggttcagca aatcaagata ttcgtagcta ccgattgaat tttgaaacaa gtgctgtcat
     7321 ttacgatcct gagtttttag aagaacttgc tactcagttc aaagaagatg agacacattg
     7381 ttcatccatg acaactgaaa cagtcaagga aatgtctaac tggctattat tcaagcaaca
     7441 aatttctcga ttattttctc caatcctata atttttttat tcaagtagag aaaaatatac
     7501 taagattaaa gggactgtga caaaaatctt gtcacagtcc cttcttccga acaaacggtg
     7561 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgtttt
     7621 ttgaacttgt tccttgtgac tcggagctgg acggcaggct cacgacctct ttc
//
LOCUS       DACEUF010000099         7660 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.103, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000099 DACEUF010000000
VERSION     DACEUF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.103"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(93..1196)
                     /locus_tag="IXG20_002323"
     CDS             complement(93..1196)
                     /locus_tag="IXG20_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1610498.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1286..2221)
                     /locus_tag="IXG20_002324"
     CDS             complement(1286..2221)
                     /locus_tag="IXG20_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ1610499.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2523..4457
                     /locus_tag="IXG20_002325"
     CDS             2523..4457
                     /locus_tag="IXG20_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1610500.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            4914..5330
                     /locus_tag="IXG20_002326"
     CDS             4914..5330
                     /locus_tag="IXG20_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1610501.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5344..5910
                     /locus_tag="IXG20_002327"
     CDS             5344..5910
                     /locus_tag="IXG20_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ1610502.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            5933..6919
                     /locus_tag="IXG20_002328"
     CDS             5933..6919
                     /locus_tag="IXG20_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1610503.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7074..>7660)
                     /gene="scm"
                     /locus_tag="IXG20_002329"
     CDS             complement(7074..>7660)
                     /gene="scm"
                     /locus_tag="IXG20_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1610504.1"
                     /translation="DTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASS
                     DSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTS
                     DSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALP
                     KTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2169 a         1472 c         1594 g         2425 t
ORIGIN      
        1 aataccccta ccgcaaaagg aaatacacta gccacctttg ttcgtatttc aactacttct
       61 aaaaagattt ttagtgtcat ggttcgtact ccttatttta gttcagaacg gtcacccatc
      121 gtgtaatggg agtcctccga taatttgaag gtgtctttga ttggatcggt cacatatact
      181 ttatcgtctt ttgtcacgaa gatcgcttcc gtcccgttat tcaattcgct ttcgacgtat
      241 tccagtcctt ttttcacacc cattgaaaag actgctgtag acaacccgtc accatcaatt
      301 gatttatctg ttatgatcgt gacacttgcg atatcattgt cgaacggata acccgttttt
      361 ggatcaaaca aatgatggta ttttttgcca tctacttcta aataacgctc gtaaattcca
      421 gaggtaacta atgttttgtt ccgttcttta atagaaccta aaacagatcc gcgagccatg
      481 ttcggatctt ggatgccgac tgtccagtct tggttttcgc ctcttgggct gtggcctaag
      541 acatagacat taccgcccaa gtcaacgatg gcagtggtca ctccttgttt tttcaagact
      601 ttcactactt cgtccgtaat gtaacctttg gcgattgcac ctaaatcgat gatcattcct
      661 ttttctttta gataaacagt tttttctttg tcatttagtt cgatcttatg ataatccact
      721 aatttcaatg cctgatcgat ttcttcttgg gaaggttttc tggcgtcatc aaaaccgatt
      781 cgccataatt gtgtgattgc accaatcgcc atatcgaatc cgccttgaga atcttggcta
      841 tattcgtagg cccgcttgac taacgtgtag acgtcatcag aaacttttac tggtttgatc
      901 ccagcttgct cattcacttc atcgatctca gaaccttttt gattgatcgt gattttatca
      961 ccaagttctt ttactcgatc gaaggcaggt ttcaatgctg attctttgcc ttcatcatac
     1021 acacggatac gaacgtaagt ccctaacaag aactgttcat cagaatatgg atctttcaat
     1081 atcttggtag ctgctgaact ttcagtggtt gttttatttg ctgagttgct gcagccagtt
     1141 agcaccataa aagagatgcc cagtgtgatt aaggaaaata gtagtttttt ccgcatcttt
     1201 ttcctctctt cattttgaaa ggtgagacga ctgcctaatc tggctatcgt cccaccctcc
     1261 aacttttatt agtatgatta tgtgattatt tttctgttac gatattatct acttcgattg
     1321 tttgagtgtc gcctttttca gctgcattca ctaattgttg tgcgtacatg atgaatgaat
     1381 gtgagctgtg tgtagcacct gtaacaactt ctacattagc aggtgagcca tcttcttcgc
     1441 ccattgcttt gacgaattcg tcgttcagag tagggatgta agtttttggt tctgtaccgc
     1501 tttttgcttt catattttca ttatattctg tatcatcttg ttttgattta ccgtctttgt
     1561 cgatataatc aaagtttgat tcagttactt tgccgccagc aactgtcatt tcgaattgca
     1621 cacggtagcc gttagagtag ttcttttctt caagcttgta agtgccgtct ttcaaatcag
     1681 caccattgtc gatttcgatt gtatctgtgt taccagcttg agcagcttgg atcaattgtt
     1741 gtgcgtagtt ttggaagctt tcagatgagt gagtagctcc agttacgact tccacaccgc
     1801 ttgcgctttg tgcttttaag aattggtcat tcaactcagg aatgtatttt tctgggtttg
     1861 tgccagattt tgcttccatg tttttgttgt attctgcatc ttttgttttt gattcgccgt
     1921 tttcattgac gttatcatat tttgattcag tgattttgcc gtcttttaca accatttcaa
     1981 agactgcacg gtaaccattt gaataatttt tttcttctaa tttatatgtg ccgtcttgca
     2041 aatctccgcc agctacaact tttgcagatg attctgttgt gctttttgca gtcgaagatt
     2101 gtgctgtaga tgattcagaa gaagagtttg agcttccgtt gtcatctgcg ccgcaagcgc
     2161 ctaacactag agctgaaaat gctaatacgg caaatccagt tacaaaacgt tttttcttca
     2221 ttttaatacc ccaccattcc atttctagtc cacaattttt ctttgtgatt cattatacat
     2281 tctaatcttt ttagcctata agtcaactat ttttgataaa taacccatgt aatatttcaa
     2341 acgttttctt tcagcaaatt tcgtgaaaaa acgctttatt ttaaaatttg aaaaaaatcc
     2401 aaaaggagtt agacaaaaga acagaacatc tatataatat atggagattg tgtaataagt
     2461 atcaaactaa aagaattatc atatattcat tatatagata gtctataagg agcggatgat
     2521 caatggcaaa gcaaaatatt gtcgttgtag gagcaggata tgccggtgtt tcagctacaa
     2581 aatacttggc aaagaaactg aaaaaagaag atgtagcaat tactctgatc gatcgtcatt
     2641 cgtaccatac tatgatgacc gagttgcatg aagtagctgg cggacgtgta gaaccagaag
     2701 caatccaata cgatctgcag catttgtttg ctcgtcaaaa gaatgtacaa cttgtcacag
     2761 acactgtaat cggtatcgat aaagaacgaa aaatcgtcaa gaccaaatta ggttcctatc
     2821 cttttgatca gctgatcctt ggaatgggcg gagagccgaa tgactttggt acacctggtg
     2881 tcaaagcaca cggattcacc ctttggtcat ttgaagattc tgtaaggatt cgggaacata
     2941 ttgagaagac agtagcaaaa gctgcattgg aaccagatga tgaactgcgt aaagccatgc
     3001 tgactttcgt tgtctgtggt tctggattta ccggtattga aatgattggg gaactgatcg
     3061 actggaaaaa tcgcctagcc aaagattata aattagatcc aaacgaaatc acactgatgg
     3121 tcgtagaggc aatgccgacg atcttgaata tgttggatcg aaatgatgct gcaaaagctg
     3181 aacgctattt gcgtaagcac aacgtggaat tattattgaa tgcaccaatc gtagaagtgg
     3241 ctgctgatca tatcaaattg aaagacggac gcaacattcc gactcacaca ctgatttgga
     3301 cagctggtgt caaaggaact tctgacgctg ctgactttgg attagaatca gcacgcggac
     3361 agcggttgat cgccaatgaa tatatgcagg caaaaggcta cgaagataaa aacatctata
     3421 tcattggtga tttagtcttt tatgaagaat tcccaggaac accgacaccg cagatcgttc
     3481 aagctgctga acaaacagga cacacagccg cagcgaatat cgtggcttcg atcaaaggcg
     3541 gagaaaagca taaatttaaa ggaaactatc aaggatttat ggtttctatt ggtgccaaat
     3601 ggggggtagc taacctattt gataagatcc atttaagcgg atttttagct atcgtaatga
     3661 aacatatcgt gaatttgaaa tacttctttg atattcgttc tggttattat atgttccaat
     3721 atttaatgca tgagtttttc catatcaaag atgatcgcaa cgtgactcga ggacattctt
     3781 ctcgttacgg caatgtctta tggagtgtgc cacttcgtat tttctatggt ttagtttggt
     3841 tcgttgaagc ttccaaaaaa atcattggga atggtgaagt gttcaaacca agtacttggt
     3901 ttggtgaagg atcatggttc actggaaaca gtgtgttccc ttggacatgg gaatacaatg
     3961 taacagatgt tgccagtggt gcttctgctg ctggtgatgc aaccacagct gccagcggag
     4021 caggggcaag tgccggagaa gcagcgactc aagctgcaca ttttggtttg agttatgcat
     4081 acggtgaaga accaatggct gtgattgaga agtcaccaga ctggttcaca aagatcatgc
     4141 aagtgatcat gccaaacaat gatgtagcaa tgttcttcca aaaattcatg gtattctttg
     4201 aaattgcctt ggcattagca ttgattgctg gattattcac atggctatgc agtgcgacaa
     4261 cgattgtatt ggtgatcacg ttctgcttat caggcatgtt ttactgggta aacatttggt
     4321 tcatttttgt tgctttcgca ttgatgaacg gttccggacg tgcgattggg ttggatcgct
     4381 gggtaattcc atggctacaa cgtacactag gtaattggtg gtacggtaga ccaagttccc
     4441 agtacggcaa gaaataaata gataaagaac gttatttgaa tgcaagattt caatagaaag
     4501 gggctgagac aaaccgtcac agcctccttt ttatgtaaaa ttaaaggaat aagatgcccg
     4561 agataagcct ccatgagcaa gacacaggtg tcacaacctc tgttacaacc tcttgttata
     4621 aacggtgttc cagaagtagc ttcttcgaaa aataagccga aatttcacaa aaacttgaaa
     4681 agtaattttc gtaaattcct tcttatttct tgaagttgcc cacttctgtc acaacctctt
     4741 gttataaacg gtgttccacc tgctgcttct cggtaataag cccaaagaat tagaaaacat
     4801 ggaaagctgt tttctattct tcgttcttat tactcaaagc aagacacagg tgtcacaacc
     4861 tcttgttttt atgctaaaat tgggtgagtt aatctggaaa aaggagaaca aaaatgagcc
     4921 ttaaagaatt tgctgaaaaa agtctgctca aaccttggga tgtttttatt attttgcttt
     4981 tgactgttct ttctttttta ccagtcatga tttttggtct tcagcaaaac accgcaacag
     5041 cagaaaaaga agcggtactg cgtgtggatg gcacagaaat caaaacattc cctttgatgg
     5101 ctggagaaga gagcaacaca tatacttaca aagatgaaca cggagattat aacttgatcg
     5161 agatagatgg cgatcgagta cggataaaag aagcagattg cgatgaccag atctgcgtac
     5221 gtcgaggctg ggcaacgaaa aatggcgaaa ccatcgtctg tttgccacat aaattagtga
     5281 tcgaagtccg ttcaacagat ggaggtgacg aagacagtct gatttattag accttcacac
     5341 accatgagta aattacaaaa aatgatttat atttcgctac ttgttgcaca aggagtgatc
     5401 attggtttat tggaaaatat gatcccttat cctttttctt ttgcgcctgg tgcaaaacta
     5461 ggcttagcca atctaatcac gatcgttgcc atttttacca tgaaaaaaca agacagcttc
     5521 ttactacttt ggctacgact attcctgact actttgctag gcggaacgat ttccacattc
     5581 ctttatagta tgagtggtgc tttgcttagt tattttggaa tgttattggt caagcaatta
     5641 ggaccaaaac gtgtcagtat catcggaatt agtgcggccg gcggtttcat gcataatgtc
     5701 ggacagcttt tgatggcttc tttcattgca caatcatgga cagtcatgtt gtacttgcca
     5761 gtcctttcag ttttagggat tttatcaggg attgcaatcg ggatcgcagc gaattatctt
     5821 ttacgacatg tgcagacact tcaacgattc caaaaagatt atgagcaggg aaccaaacaa
     5881 aaatggagcc caatgtttac caaaaaataa tcgtcacaca aggagttttt ttatgaagct
     5941 acatccaatg tgggaaaact atcctgcttt aagtaaagaa ttgacagaaa cactaaaacg
     6001 gatggaagaa actgtccgcc tgaaaaacaa acctgtcgaa caagcaatca aagaaacgat
     6061 ccatgccggc ggaaagctgt tgcgtcctgc ctatcagcta ttgttctcac aatttggacc
     6121 agagcaagat cgagacaaag ccatcgcttt ggcagcttct atcgaaatgc tccatacggc
     6181 cacactgatc catgatgata tcgttgatga agcggatctg cgacgtggtc agcctaatat
     6241 ccgtagtcag tttggtaatg tcgtttctgt ctatgcagga gactatttat ttgtctgctg
     6301 ttttaaacta ttgtctgatt actccacttc cttgaaaagt ctccaattga attctcgcag
     6361 tatggaaaaa gtattgaatg gcgaattagg gcagatggat gatcgctata attatgaact
     6421 ttctgtaaaa gaatatttgg aaaatatttc aggtaagaca gctgaactgt tccaattaag
     6481 ttgttctgtt ggcgcttttg aaagtggaac aagcgagcgc ttcgcaaaaa aagcaggaga
     6541 tatcggactt tctatcggga tggcctttca gattatcgat gatattttag attatacaaa
     6601 agagagccag gaaatcggca aacctgtact agaagacatg cgtcaagggg tctattcttt
     6661 accgttgatc tactctttgc aaaaaaacaa accaaagcta ctaccttatt tagagaagaa
     6721 agcggcaatg acagaagaag atgtcgacgc cgtacgcaaa atcgttgaac atacaggagg
     6781 cgtggaagaa gctaggaagt tagcggcctc ttacacagaa aaggctttga aggaaataaa
     6841 gaaattacct gccacctctc ttcgaacaaa agagaatctc ttttccctta cccaactgat
     6901 tttggatcgc aaagactgaa tagggaaagg ttcgatacgt aaataaattt ctcagaagga
     6961 aatctacgtg ttttttgaca aattgaattg tagttcttgt atttatgata aaaagcaaaa
     7021 ataaagagca acgtccgtta gcgacgttgc tcttttttat cttgaatatt tttttacctt
     7081 ttcgatttct tataactcat aaaagtgatt cttaaaccga ttacaagtaa tataacgcct
     7141 gcgagtgtta tccaattatt cgattgactt ccagttttag gaagtgctct ttttgatttg
     7201 tttccattgt cattgcttgt tttattagtt aagttaaagt tcgtcctgtt atccgaactt
     7261 gtttttgaga aggagacact tgcatgacgt gtatcgcttg tggtagacga cgtgtctgta
     7321 ctatcgcttg aagttgatga atctgagcta tcacttgtgg tagaggatgt atctgtactg
     7381 tcacttgaag ctgacgtgtc cgtgctatca cttgtagtgg tagatgaatc tgagctatca
     7441 cttgtggtag aggtagtatc tgtactgtca cttgaagctg acgtgtccgt gctatcactt
     7501 gttgtggtag atgtatccgt actgtcgctt gaagctgacg tgtccgtgct atcacttgtg
     7561 gtagaggtag tatctgtact gtcacttgaa gctgatgtgt ccgtgctatc acttgtggta
     7621 gaggtagtat ctgtactgtc gcttgaagct gacgtgtccg
//
LOCUS       DACEUF010000100         7623 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.104, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000100 DACEUF010000000
VERSION     DACEUF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.104"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(14..706)
                     /locus_tag="IXG20_002330"
     CDS             complement(14..706)
                     /locus_tag="IXG20_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1610505.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IXG20_002331"
                     /pseudo
     CDS             791..967
                     /locus_tag="IXG20_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IXG20_002332"
     CDS             complement(1143..1712)
                     /locus_tag="IXG20_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1610506.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IXG20_002333"
     CDS             complement(1786..2109)
                     /locus_tag="IXG20_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610507.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IXG20_002334"
     CDS             complement(2263..3075)
                     /locus_tag="IXG20_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610508.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IXG20_002335"
     CDS             3429..4277
                     /locus_tag="IXG20_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ1610509.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IXG20_002336"
     CDS             4422..5363
                     /locus_tag="IXG20_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ1610510.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IXG20_002337"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IXG20_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1511 c         1371 g         2469 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aac
//
LOCUS       DACEUF010000101         7623 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.105, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000101 DACEUF010000000
VERSION     DACEUF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.105"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(137..1147)
                     /locus_tag="IXG20_002338"
     CDS             complement(137..1147)
                     /locus_tag="IXG20_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1610511.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(1159..2112)
                     /locus_tag="IXG20_002339"
     CDS             complement(1159..2112)
                     /locus_tag="IXG20_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1610512.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(2102..3172)
                     /locus_tag="IXG20_002340"
     CDS             complement(2102..3172)
                     /locus_tag="IXG20_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1610513.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(3178..4221)
                     /locus_tag="IXG20_002341"
     CDS             complement(3178..4221)
                     /locus_tag="IXG20_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1610514.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(4222..5163)
                     /locus_tag="IXG20_002342"
     CDS             complement(4222..5163)
                     /locus_tag="IXG20_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1610515.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            5247..5594
                     /locus_tag="IXG20_002343"
     CDS             5247..5594
                     /locus_tag="IXG20_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ1610516.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(5664..7325)
                     /locus_tag="IXG20_002344"
     CDS             complement(5664..7325)
                     /locus_tag="IXG20_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1610517.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         2131 a         1574 c         1379 g         2539 t
ORIGIN      
        1 tttttctgct attgttttat tggtttgtca taattagtga gcttttcttc aaaagaaaaa
       61 ttcctagaaa caaaagtcat taagactttt tattaacaag gctaacccta acgctcctat
      121 cagtttcatt cattttttat cccttgataa ccgaatgtct gcccattttt tctactactt
      181 ggctctttct tacggaattg ctttcttctg ctccgtcggt attgatcaaa agaaattttt
      241 tattatacca ttgggactta taacgcaatt ttgttattaa tttatgccag tcgtgaatag
      301 aaaaatcagc atgtgcctct tcttttggaa aagattctac aaataagtag tcatattgtt
      361 tgaccaattt cccagataat tgttcaagat aatcttctcg ctggttcttt tgtttcagat
      421 aaagatccat tacttttctc ttttgtttct gataattgct tgcctgatct aacctgatct
      481 ttctatggtg agcggagcgg actttcacat tcaatttccg ctgtgcatgt tgcagctttt
      541 cttctgtctg gtgaaatttt ggtaaagtta cttctagagg tcgagatgtt tggatcaatg
      601 tcttcggatg ataggcgatc ccgatccatt tagctaaaga ttcttttttt actggcgaaa
      661 catcacaaag tagtgcgaca taaaattctt cattatatcg agcagaaatc gtagcggagc
      721 gaatcgatcc ttcaactggc cgatgctgat ttatcttgat tagttctttt tgttttggta
      781 actttaagta gccattttcc agatagatcg tacgggtttg gttgttggtc gtataagatt
      841 gccacatact ttttttcgtt ttaagctttg ggaagctggc acgtcccttg aaataattgc
      901 gaaaagcccg atccaaatta agctgtgcat ttgctaaagc aagactgtct acttctttta
      961 agaatgggta ttcttttttt aatgtagcgg gtgtgagcga aaaattggct actcctgttt
     1021 gttgccaact tttctgccgc tttttcagca aatggttata ggtaaatcga gcacatccaa
     1081 aagtacgaat caattgctgt ttttgttctt ctgtcggata catacggaat ttaaaagctt
     1141 ttagcatcta tgtccctcct agttagcaac cagaagagat tccttttgtc gttttagttt
     1201 ttttgcgtaa tatgggactt cctgctcagt tgcatagaca aaatgttctg gtgcgatttc
     1261 acgaagttga cgtacttgtc cgtcatctgg ttcaggttga tatttgattc ttctgcgttt
     1321 tctctcatgg tctggatctg gtagaggaat agcagataac aaactttctg tataaggatg
     1381 aacaccgtga ttatagattt catcacttgt tcccatctct aataacaagc cgttatgcat
     1441 tacaccgatt cggtcactaa tatgtttaac catcgacaaa tcatgtgcaa taaatagata
     1501 ggtcaattga tgttcttttt ggagatcttg caacaaattg accacttggg cttggatcga
     1561 cacatctagt gcagaaattg gttcatcaca aatgataaaa cgcggtttta ctgccaatgc
     1621 tcgtgcaata ccaatacgct ggcgttgccc acctgaaaat tcatgaggat aacgagtacc
     1681 gtggcttgga ttcaatccaa cagttctcaa tagatcatcg actttttttg cacgctcttc
     1741 agatgattta gctagtccat taacatcaat tccttcggca atgatatctc tgactttcat
     1801 acgaggattc aacgaagcgt atggatcttg aaagatcatc tgaacatcat gccggaattt
     1861 cgtcaaacct tttttatctt tgatcttcgt tatatcttga ccgtcgaata atatctcacc
     1921 atcagtaggt tcattcaagc gaatgatcgt acggccagta gtggattttc cgcttccaga
     1981 ttctccaact aaaccgaatg tttctccttc ataaatgtga aagctgatgt catttacagc
     2041 atgtacttca ttcttttttc ctacattgaa atactgtttc aatcctttaa cttcaagtag
     2101 tttatttggc atcagcaatt cctccttgac cgtaagtatg tttttgtttc tctttaaaga
     2161 tagcccagcg acgttggatt tcttctggcg gtgtcacttt gggtgcctgc ggagcaagta
     2221 gccaagttgc tgccttgtgt gtttttgata gttcaaaaaa tggtggttct tgttctgcat
     2281 cgattttcat cgcgaattca tttcttggat agaatgcgtc tccttttggc gggttcaata
     2341 aatccggtgg agaaccagga atagcataca atcgatcgtt cgcactatct aatgtcggca
     2401 ttgaaccaag cagtccccaa gtatagggat gttgcgggtt gtagaaaatt tcttccgatg
     2461 ttccaacttc aacgattctt cctccataca tgacagcgac acgatcagcg acattcgcta
     2521 ctacaccaag gtcatgagtg ataaagataa ttgatgtatc gatttttgtc tgaatttctt
     2581 tcagcagctc caaaatctgt gcttgaatag tcacatcaag cgctgttgta ggttcatcag
     2641 caatcaggat ttccggataa cataccagtg caatcgcgat cacgatccgt tgacgctgac
     2701 ctcctgagaa ctgatgcgga taactgttga tacgtttctc agcttcgggt attccaacta
     2761 atttaagcaa ttctaatgca gctttttgtc cctctttttt tgaaacttta ttatgctttc
     2821 taagggactc agcgacttgc ttcccaatgg gcatcgtcgg atctaaagag gtcattggat
     2881 cttggaaaat catcgctatt tctttaccac ggattttttg catctctctt tctgattttc
     2941 caacgatatc gttaccttta aagaggattt gaccagcatc tatattggcg ttgcttgaca
     3001 gcaagcgcat gatgcttctg gtcgtcactg attttccact tccagattcc ccaacgatcg
     3061 ccaatgtttc tcctttattc aaatgaaaat tgacaccgcg aatcgctcgt acctttccag
     3121 catacgtatc aaaagagatt tctaagtctt tcacttctaa aattttcgtc atcgtcttca
     3181 ctctttcatt ttaggatcaa aggcatcacg caatccatca gcaagtaagt taaagccaat
     3241 catgataacc gataaagtta ctgccggaat ccataataga taaggcagat acaggaatgt
     3301 cttgtaccct tcgctcaaca tcgttcctaa tgaagctgac ggaggcgtaa gacctaaacc
     3361 gataaagctt aaaaaggctt cgaagaaaat agcagatgga atactaaaca tcatttgaat
     3421 aataatgaca ctagagatat tcggcaaaat atgtttgaat gcaattttca ttcttgattc
     3481 acctaatgtc aatccagcta aaacatattc ttgatctttt aatttcattg tttgtgcacg
     3541 aacgatacgt gccattggta tccagtttgt gatggccata gcagccacga ttgaaaaaat
     3601 tcctggttca aaaacgacca acatcaaaat catgacaacg aggtttggaa ttcctgataa
     3661 gatttccaaa aaacgttgca tcacgttatc gacacgtccg cctaacaaac cagaaaccaa
     3721 cccgtaagct acaccaatcg ttacatctag taatgcagca ataaatgcga tcagcaacga
     3781 gattctcatt cccataaata agcggcttaa tacatcacgt cccaaaccgt ctgttcctaa
     3841 aaagaaattt acgtttgaag ggacgtttgc ttgagcatat ttatcaacaa gttcaccacc
     3901 aaccattgct tttccattca atccatcgat attgattccc ggaatacgag gaggcaaatt
     3961 gatataagcc acgttttggg cagttggatc atgaggagaa acaaagattg tgatcacaga
     4021 gatcaaaatg atgaccaata gtaaaaccat tgaaataacg gcagctttgt ttttctttaa
     4081 acggcgccat gaatcttgga taaaattcaa agaaggtgca gtaatctgtt cacgttcttc
     4141 ttcggtattt ttttgtaatg gaagaaattc atccgcaggg atatctgcaa ctgtttcata
     4201 acgctttggt atcatttcca tttatcctcg gctcccttct gaaacacgaa ttcttggatc
     4261 aactagaccg tacaatagat ccacgatcaa aataacaaaa atcagcataa atgagtaaag
     4321 gatcgttaca gccatgatcg taggataatc atttgttgtg attgacttaa cgaattgttc
     4381 cccgatacca ggaatagcaa agatattttc taccaccagt gatccggtca tcaatgctac
     4441 agctaacggt cctaataaag tcatcaatgg aatcaaacta tttcgaagac catgtttaaa
     4501 ggcgatttcc caacggctca atccttttgc ttttgctaat tccacataat cactatgcag
     4561 tacttccacc atttccgtac ggataaaccg agcagagtca gcaagcggcg acatggccaa
     4621 cgcaattgtt ggtaagattg tgtacacaaa gccatcccat tttgcgattg gcaaaacttg
     4681 taatttgatt gcaaagatat attgtaataa aacagcaaat acaaagtttg gaatggaacg
     4741 acctaatatc gctacgagtg tagaagatgt atctgcccat gtattttgct tcatggcaga
     4801 gatcgttcct aaaatcgtcc ctaggactgt tccaaagata attgcctgta accctaactg
     4861 caaggatggt ccgattcttc cagctaacaa atgtgcgact ggctggtttt taaattggaa
     4921 ggaaattcca aaatctcctt gaagcaaatt agaaagataa atcccatatt gtacgataac
     4981 tggtttatcc aaaccgactt gtttatttag catctcgatt gtttcaggac tcaaacgttc
     5041 ctgattcgta tatggcgtgc ccggcagcaa ttgcatcaag aaaaaggtga ttgttgcaat
     5101 taaccaaagc gtgatgatca tgaaaaagac acgttttaat acatatttga tataactatt
     5161 cactaatgtt tccccctagc ttttgacttc tgacagatgt ttcgctacat tattagataa
     5221 tgaaccaaaa ataaaggagc aatcaaatga aaaaaaaatc tatcccctac gcagtagctt
     5281 ttctactgat tctggttatt ttaataaaaa atctcatcaa ccattccttt acactcattc
     5341 aattatctaa tgatctattc ttatggtcat tgccgtttct gattatcggc ggttttttat
     5401 gggtcttttc ttcgggtttc ttcgatcatt ttcagcgatc ggtccatctt gcacgaacaa
     5461 ggaaccgaaa gaaaaaacca gaattttcct cgctctcgtc tgccagctac ggtatgtaca
     5521 gcttttggct gatcattgcc ggaatactaa ttgctctttc tgctattttc atgctttttt
     5581 ctttgttagg ataagtgaat atgagaacga ggttgcgaca tttgccgcag cctcgttttt
     5641 tctcatgctc acggtattaa cgattattct tcaacgtatg tccatttgta atcccatgaa
     5701 gcgcctgccg catggtgaac gatacctttg attttttcat tcactaggtg accttctgct
     5761 ttttggtaca ccggaacgac acccatatca tcagagatga tcttgattgc tttttggaaa
     5821 tcatcccaac gggcttgttc atcgccagcg tctgttgatg aagctgcttt aactgcttcg
     5881 tcatattctg cattgctcca gcgaccacgg ttgtatgaat tacctgtgac aaacaaatca
     5941 gtaaagctgc ttgggtctgc atagtcagca gcccatccgc ctagtactgc gtcaaaatca
     6001 cccgaagttg agcggtcaag acgtacagag aatggaacag gtgttggttt gactgttacg
     6061 ccatccaagt tttcttggat tgagttttga acatattcga ttactttttt cgatgaatca
     6121 tcatcagaag ccatcaaatc aaattctaaa gaatcaacgc ccaattcttt tttcgctttt
     6181 tcccaatatt cttttgcttt atctttgtca taagaaacta attttccagc ttcttttgca
     6241 aagtcttcgt tcgttgttgg actttttacc atatctgatg ggatcaatcc tgtagagaca
     6301 actgatccgt cacctagtac ttgttttact aaagcttcac gatcgataga ataagaaata
     6361 gcttttctta ggttcacatt gttgaatggt gaattctttt cacgttgatt gaattcgata
     6421 tagcttgtcc gcgcttcttt aacagaagta taagctggat cgtttgcgtt ttgctgtgct
     6481 aactcaccag acaagatcac gtcatctgct tggccatctt tgaataagtt caatgcagta
     6541 gaagattctt ttactacact gactttgatt tcagaaagtt ttactgtatc tttgtcccag
     6601 taatgatcat ttttcttata agtccattct gtatctgttc ctggtccatc aaaatcttct
     6661 aatgtgaatg gtccattata aacagcttta tcacttgcag atgcataggc atccccattt
     6721 tcttccacta cggattgttt ttgtgggaaa aatgatggga aagcaagcag atattggaaa
     6781 tatggtgttt gtttcgtcaa tttgatttct aattcatagt caccaactgc tttgatccct
     6841 agttctgatt tatctttctt tccagctgta atgtcttctg cattttcaac tggcgcatac
     6901 aggtaagcat attcagaagc tgtagctggg tctactgttc gttgccagcc ataaacgtaa
     6961 tccgctgctg taacaggatc accatttgac cattttgcgt cttctcgtaa tttgattttg
     7021 taagttaatc cgtcttcact gacttctgcc aactcgctag ctcctgccgg ctgtggtttg
     7081 ctgtcttcat caagacggta aattccttca taaacgttat tcaatgctgt aaaactgatt
     7141 gtatctgttg caactgataa gtcagctgta ggcatttctt gaggcacgac taagttaaat
     7201 acgcctcctc catctgctga accgcttttt gctgaactac tgcttgtggc tgaactgccg
     7261 ccataacatc cagcaagtac aaggccgcat accgcaacca aaccaaatgt aaatttcttt
     7321 ttcattttac gtaccccttt attggaattt tcagaataat taatattttc tgaagttgtt
     7381 taagagttta atgaataatt attaaaaaat caacactttt ttttgtcttt ttcataaaat
     7441 aatgattttt tcgtgaggtt tcaaggaatt tttcagaaaa aatagctttg tacaaggttt
     7501 tccatggtct tccaaaagcc attaaataat tttcgttata ttgtctatta ttgccataca
     7561 aagataaaaa agaataaaca aaaagaaact gtgacaaaag tcttgtcaca gtcccttatc
     7621 tga
//
LOCUS       DACEUF010000102         7597 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.106, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000102 DACEUF010000000
VERSION     DACEUF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.106"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(135..425)
                     /locus_tag="IXG20_002345"
     CDS             complement(135..425)
                     /locus_tag="IXG20_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610518.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(543..803)
                     /locus_tag="IXG20_002346"
     CDS             complement(543..803)
                     /locus_tag="IXG20_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610519.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(940..1353)
                     /locus_tag="IXG20_002347"
     CDS             complement(940..1353)
                     /locus_tag="IXG20_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ1610520.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="IXG20_002348"
     CDS             complement(1809..2294)
                     /locus_tag="IXG20_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610521.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2648)
                     /locus_tag="IXG20_002349"
     CDS             complement(2433..2648)
                     /locus_tag="IXG20_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ1610522.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2872..3672
                     /locus_tag="IXG20_002350"
     CDS             2872..3672
                     /locus_tag="IXG20_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1610523.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3691..5559
                     /locus_tag="IXG20_002351"
     CDS             3691..5559
                     /locus_tag="IXG20_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1610524.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLKLAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5552..6043
                     /locus_tag="IXG20_002352"
     CDS             5552..6043
                     /locus_tag="IXG20_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ1610525.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6031..6585
                     /gene="srlA"
                     /locus_tag="IXG20_002353"
     CDS             6031..6585
                     /gene="srlA"
                     /locus_tag="IXG20_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ1610526.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6603..>7597
                     /locus_tag="IXG20_002354"
     CDS             6603..>7597
                     /locus_tag="IXG20_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ1610527.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2234 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaaa
     2641 gttttcatta gaattctcct ttcttaaatg ataaaacaat tatatatcaa aaagagaaac
     2701 attgcttata taacgctcag gaattttagc aatctatgca aaaagatgaa aatttttttt
     2761 gctcttccat tagtacacat tttttagctg aagctgtaaa tttcatgtct taattttttt
     2821 tagtgagctt ttatactgga tttgtaagaa aaaagaagga gaggatcaca tatgtcggat
     2881 tggttggata ttgcaggaaa agtagtaatt gtaactggag gttcctcagg aattggaaga
     2941 agtatcgtcg aaaatttgtt gaagcagaat gcacaagtag cgaattttga tgtaactgaa
     3001 tgccgtatac agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc taaaacggac
     3061 atagaagaag ggatatataa agtaatgaaa catttcaaga cgatcgatgg tctagtcaat
     3121 aatgcaggaa taaatattcc ttctcttcta atagatagaa atcatccaaa aagcaagtat
     3181 gaattatcag aacaagtttt tgataagatg atcgctgtca atcaaaaaag tgtatatttg
     3241 atgagccaag cagttggaag aatcttggtg caaaagggat caggagtgat cgtgaatctt
     3301 tcttctgaat caggattgga aggatcagaa ggacagagct gttatgctgc tacaaaagca
     3361 gcgatgaaca gttttactcg ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg
     3421 gtaggtgttg caccagggat actggaagaa accggtttga gaacacaaga atatgaagaa
     3481 gctctttctt atacgagagg tataagcgtc gaacagctga gaaacggtta cagtcgaaca
     3541 agtacgatcc ctctaggaag gagtggtaag ctgcaagaag tggcggatct agtctgctat
     3601 tatctttcag aaagatcaag ttatatcact ggagtcacga ctaatatttc aggaggtaaa
     3661 acaagaggat aaaggatagg ggggacgcac gtgacgctag taaacagatg gtaccaatta
     3721 ttgaaattac tagtcactca caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa
     3781 accagtaatc aaaccataaa aaaagatatt ggggagttga atgaagaaat tgccggtatt
     3841 gcaaaaatca tccaaaaaaa caaacatttt gaacttgaaa tttatgattt tgattttttt
     3901 gatgagatca tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc atccaaaaga
     3961 atcgcttatt tactaaatag gctgatcgaa acagaggatt tcttacgtat ggatgatttg
     4021 tctgaagaag tcggtgtcag cagaggaaca gtggtcaagg atctaaagac attaaaagag
     4081 atgatcaatg attttgatgt aaagattaca ggcacaccaa ataaaggact aagaataata
     4141 ggagatgagc tagaattacg actactttta ttttattttg tttctcccta cttttcagag
     4201 acgaaaacag aacagtttct agaagaaaaa caagcaatca aacattttca gaagaagacg
     4261 aatgctagta aacaagaaat caccctttta acgaaagtta tcggtatttc tttgaataga
     4321 atctctagta actatctgtt gaagaagccc atcaatcgtt acagtgggtg ttttgaatat
     4381 atccaagaat tcaatgaact gatcaatcag ttggaagaaa tctatgaact aactttgagc
     4441 caatatgaaa aagattttat cagttttcct ttgaacatca tcaacacaag tataacgatg
     4501 ataaggaaag ctaactctga tttgcgctta tatttcgacc ccatgatgat gcgtattcga
     4561 cgattagatc tcattgatct ggatgagtcc ttcttatatc aagagatgaa acagcacctc
     4621 tctttattga taaatcgggt aatttttcgt tatcgattga ctgatttatt ttatggtgag
     4681 atagaaaaaa agtatccttt ttcttaccaa atcgctagcg aatgcataca agcattagaa
     4741 gctatcttgc aacataagat ctctaaagta gagatcagct acctaacgtt gtatttgaag
     4801 ttagcatttc gatatcaaaa agagcatttt tcctctaaaa aagtagccat tgttcatacg
     4861 acaggaaaag gaacagcatt aatgatccaa agacaaatca aaaggatatt ggggagggat
     4921 atacaaataa cacatgtttc agaagaacaa tatgaaaaag tagatctaaa caaatatttc
     4981 gctgttttta caacgattcc gttaaaaaat gtaaatgaag agacaccggt gatccatttg
     5041 actaatcttt ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga
     5101 ttagccgggt ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat
     5161 caagaatatt taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa
     5221 tttgaaaaaa ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt
     5281 gcttttcctc acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata
     5341 actgatcaag gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct
     5401 gaagaaatga cggctgaagt tgagaatgaa ttatttgagc tatacgatat cctatttgaa
     5461 ctcataaaaa tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta
     5521 aaactaatca aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt
     5581 cgcagcttca gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa
     5641 tcaaacatat agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa
     5701 aatccgttca ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt
     5761 cagaaaaatg caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg
     5821 tgaagatatc cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac
     5881 acaagcagct ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa
     5941 aagtgaacaa ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag
     6001 tacgatcaaa gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga
     6061 tttatgaggc tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta
     6121 ccagttgtct tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac
     6181 cgagtaacga aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta
     6241 ccgttcttat ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt
     6301 ccagaatact acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat
     6361 ggtgtattcc cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg
     6421 atcgaaacct tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg
     6481 ctgatgaact ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag
     6541 caaggaatcg ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt
     6601 aaatgtatgg tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta
     6661 ttaggccgac aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa
     6721 tgatcgtaga taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat
     6781 caatacctga tagtgaaatc gcgcttgcaa tcgttgactg tggagggact cttagatgtg
     6841 gaatttatcc taaaaaaggg ataccaacca tcaatatcgt ttccacagga aaaagtggcc
     6901 cattagcaca atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac
     6961 agccactaat ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa
     7021 agtacaaata tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga
     7081 tagcaaaaat tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag
     7141 aagcagtaga tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga
     7201 ttggtgtgat acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag
     7261 caggaaatgc agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc
     7321 ctttgcttgg tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga
     7381 ttgggaaagg aaatattcct ccccaattag cattaccggc actatttgct atcaatactc
     7441 aaaatgcatg tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag
     7501 tagaagtagg tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta
     7561 tcattgcttg ggtagcaagt tttggattat atagctg
//
LOCUS       DACEUF010000103         7396 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.107, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000103 DACEUF010000000
VERSION     DACEUF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.107"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            161..391
                     /locus_tag="IXG20_002355"
     CDS             161..391
                     /locus_tag="IXG20_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ1610528.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="IXG20_002356"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="IXG20_002356"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1610529.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="IXG20_002357"
     CDS             1802..1978
                     /locus_tag="IXG20_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1610530.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="IXG20_002358"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="IXG20_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ1610531.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="IXG20_002359"
     CDS             2593..3060
                     /locus_tag="IXG20_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1610532.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="IXG20_002360"
     CDS             3145..4029
                     /locus_tag="IXG20_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1610533.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4146..4379
                     /locus_tag="IXG20_002361"
     CDS             4146..4379
                     /locus_tag="IXG20_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ1610534.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="IXG20_002362"
     tRNA            4467..4547
                     /locus_tag="IXG20_002362"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="IXG20_002363"
     tRNA            4554..4625
                     /locus_tag="IXG20_002363"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            5140..5847
                     /locus_tag="IXG20_002364"
     CDS             5140..5847
                     /locus_tag="IXG20_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1610535.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="IXG20_002365"
     CDS             complement(5907..6551)
                     /locus_tag="IXG20_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ1610536.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="IXG20_002366"
     CDS             6678..6965
                     /locus_tag="IXG20_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610537.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="IXG20_002367"
     CDS             7073..7393
                     /locus_tag="IXG20_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1610538.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
BASE COUNT         2450 a         1188 c         1593 g         2165 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtc
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaata
//
LOCUS       DACEUF010000104         7385 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.108, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000104 DACEUF010000000
VERSION     DACEUF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.108"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(1..1155)
                     /locus_tag="IXG20_002368"
     CDS             complement(1..1155)
                     /locus_tag="IXG20_002368"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ1610539.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1340..3781
                     /locus_tag="IXG20_002369"
     CDS             1340..3781
                     /locus_tag="IXG20_002369"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ1610540.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(4111..4815)
                     /locus_tag="IXG20_002370"
     CDS             complement(4111..4815)
                     /locus_tag="IXG20_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610541.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(5002..5247)
                     /locus_tag="IXG20_002371"
     CDS             complement(5002..5247)
                     /locus_tag="IXG20_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610542.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(5264..6115)
                     /locus_tag="IXG20_002372"
     CDS             complement(5264..6115)
                     /locus_tag="IXG20_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1610543.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            6281..7285
                     /locus_tag="IXG20_002373"
     CDS             6281..7285
                     /locus_tag="IXG20_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ1610544.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         2329 a         1325 c         1527 g         2204 t
ORIGIN      
        1 ttatattttg tagtaacgaa tatcattttc gatctttgta atcgaaaaag gtacatctga
       61 agtatattct gcacattctc cgtgttcagg aagcgtctca ctgaaaatcg tttcatattc
      121 gtcaacagtg atccgtgttc gagaatctag catctcttga tgagattgtg cgaagagata
      181 ttcttgatag ttttcttcca gataacctgt aaaaaattca cttactgcac cagaaccgta
      241 gctgaataga ccgatgcgat ctcctggctg tagtgatttc gaattttcca gtagagaagt
      301 tagccccagg tataatgaac cagtgtaaag attaccgatt cgtcggctgt aacggatgct
      361 ttcttcatag cgagccataa gacgttcctg attttcttcg tctgtttggt ctaatacgct
      421 ttgcaatgcc ttttttccca tcttagtata cggaatatgg aaagcaatcg cctgataatc
      481 ttcgagtcct cgacccgaca attctttatg tcgattccaa actttttgga atgattcgat
      541 atacgtagaa ttagataaag gaccatcaac aacaggaaat tcgctataat ctggacgcca
      601 gaaatcatag atatcttctg tcagaaatac gctgtcgtct tcaatcgata aaatacgcgg
      661 gttttgagtg atcatcatcg caacagcacc gacaccttgc gtcacttcac caccgcttgc
      721 caagccgtaa cgagcaatat cacttgctat gactagtact tttcgttctg gatgattttt
      781 gacatattct ttcgccatgt gcaaagctgc ggtgccaccg tagcaagcct cttttacttc
      841 aaaagaacga gcaaaagatt ggattttcag taaatggtgg ataattaccg cactcgcttt
      901 tgaatgatca atcccggatt ctgttccgac gatgaccatg tcgattagct cacggtcttt
      961 ttctgttacg attttgctgg cagcatttgc tcctagtgta atgatgtctt catttgcacg
     1021 attgactgcc atttggtctt gaccaatccc tatatggtat tttgcaggat catccccacg
     1081 gctttctgcc aactctgtca tatctaaata taaattagga ataaaaaagg aaagacgatc
     1141 aatccctatt ttcatttcaa cacctcttta cttatgataa aaaacttact ttactaaggt
     1201 atcataaaaa gattaaaaga aatataaaaa agagaatatt taaggattgt taaaatttaa
     1261 tcttagaaaa aaataactgg aacaagagct atttgttttt acagcaaaga atggtatatt
     1321 tttgtaggag gtggcttact tgaaagaagt cgttatgata gatgctgcaa gaacgccgat
     1381 aggaaagtat cgcggaagtt taagtccatt tactgcagtt gaattaggca cacttgtgac
     1441 taaaggatta ttagataaaa caaaactgaa aaaagataaa atcgatcaag taatatttgg
     1501 aaatgtcctg caagctggta atggtcaaaa tgttgccaga cagattgctt tgaacagcgg
     1561 tctccctgta gatgtcccag caatgacgat caatgaagtt tgcggatctg gtatgaaagc
     1621 agtgatctta gcacgccagc tgatccaatt aggtgaagca gaacttgtga tagccggcgg
     1681 aacagagagt atgtctcaag cacctatgct gaaacagtat cagtcagaaa caaatgaata
     1741 tggtgaacca atttccagta tggtcaacga cggattgact gacgcatttt caaatgcaca
     1801 tatgggatta accgcagaga atgttgcaac acaattttct gtgagcagag aagagcagga
     1861 tcgctatgcc ttgtcgtccc agttgaaagc agcacatgct gtcgaagccg gtgtattttc
     1921 tgaggagatc atcccagtca agatttctga tgaagacgtg ttatctgagg atgaagcagt
     1981 tcgtggaaat agtacattgg aaaaactggg cacgttacgt acagtattct cagaagaagg
     2041 aactgtaaca gcaggaaatg cttccccgtt gaatgacggt gcctctgtgg tgatccttgc
     2101 atccaaagaa tacgcagaaa ataataatct gccttattta gcaaccatca aagaagtagc
     2161 ggaggtcggt attgacccaa gtatcatggg aatcgctcca atcaaagcca ttcaaaaatt
     2221 gacagataga tcaggaatga atttatcaac aatcgatctt tttgagatca atgaagcatt
     2281 tgcagcttct tctatcgtag ttagccaaga actgcagtta gatgaagaaa aggtcaatat
     2341 atatggagga gcaatcgcat taggccatcc aattggtgca agcggtgcac gtatcttgac
     2401 tacattagct tacggattgc ttcgtgaaca gaagagatac ggtattgctt cattatgtat
     2461 tggtggaggt ttaggcttgg cagtcttgtt agaagcgaat atggagcaaa cacataagga
     2521 tgtgcaaaaa aaaaagtttt atcagcttac gccatctgaa cggcgcagtc aattaataga
     2581 aaaaaatgta ttgacacaag aaactgcgtt gatttttcag gaacaaacac tttctgaaga
     2641 gctctccgat cacatgatcg aaaaccaagt gagtgaagtg gaaatcccaa tggggatcgc
     2701 gcagaatttt caaatcaatg gcaagaaaaa atggattccg atggcaacag aggaaccatc
     2761 tgtcattgca gctgcaagta atggtgcaaa aatctgtgga aatatctgcg cagaaacacc
     2821 tcagcggctg atgcgagggc aaatcgttct atcaggaaaa tcggagtatc aagcagtgat
     2881 caatgcagtt aatcatcgaa aagaagagct gattctttgt gcaaatgaaa gttatccttc
     2941 aatcgtgaaa cgcgggggcg gtgttcaaga tatctctact cgggaattca tggggagttt
     3001 ccatgcatac ttatctattg attttttagt ggatgtaaaa gatgcaatgg gcgctaacat
     3061 gatcaactcg attcttgaga gtgtggcaaa caagttacgg gagtggttcc ctgaagaaga
     3121 gattctattc agtattttaa gtaattttgc tacagaatca ttggcttctg cctgttgtga
     3181 aatcccattc gaacgcttag ggagaaacaa agaaatcggt gaacaaattg ccaaaaaaat
     3241 ccaacaggca ggggaatatg cgaagcttga cccttaccgt gcagcaacgc acaacaaagg
     3301 gatcatgaat ggtatagaag ctgtagtggc agctacagga aatgatacac gtgcggttag
     3361 cgcttcgatc cacgcctacg cggcaagaaa tggattatac caaggattaa cagactggca
     3421 gatcaaaggg gataaactag ttggaaaact aacggtgcct ttggctgttg ccacagtagg
     3481 cggagcttcc aatatcctgc caaaagcaaa agcatcttta gctatgttag atattgattc
     3541 agccaaggaa ctagcacaag taattgctgc agtagggtta gctcaaaacc tggctgcttt
     3601 gcgtgcatta gttacagaag gaatccaaaa aggccacatg ggacttcaag ctcgttcatt
     3661 agcgatctca attggggcta ttggtgagga aatcgagcaa gttgcgaaaa aattacgtga
     3721 agcagaaaag atgaaccagc agacagctat acaaatattg gaaaaaatcc gggaaaaata
     3781 aaaaagcaat atctttagaa gaataaagca agatttacag ttattttatt tactgcagat
     3841 cttgcttttt tctctatcag aaaatatgtt tattattaga attttttata gttaaggtta
     3901 ggcatcttaa gaaatcaggc aactccaaga tatcttcagg tagaagagaa gtcgcctaac
     3961 attgtaggat cataggtgat ttgttggaca ggtcaagctg atatttgagc ctgatgttag
     4021 aaggaatttc tttttacttg ctgtagaaat tattatatga ctaaaaaggc tgggatttta
     4081 aatcccagcc ttttatgaca aatagaattt ttatcgatca gtaatcgctg tcgctgcaaa
     4141 ttgttcgatc aaaggaatca aagtcaattt gactttgtat ttccgttcga gtgctttttc
     4201 gataacgaac tcattttcag tagtcataga aatagttgct aatttacgag gttcatcagc
     4261 atttttttca ttaataacta cttcatgttc attttgttct gtgtaaaatt taggatactc
     4321 agcaacggct tttgaaaaat cagcaaaact taaagcggca ttcgtatcag ttgagcgtgt
     4381 attttcagtt gtgtccgctt ttggtttttc cgttttttct tctggcacac tgactgtttc
     4441 ttctgtaact ggtgtcgcaa cagcttgcgc agttggttca tcgtttttca atcgttgatc
     4501 attccagtaa gacacgattt caataagaag attgtcacgg aatcgttcga aaatcccgtt
     4561 ttctcctgaa cgaattctct ttaatacagt attgaagttg tcatcgattg gcacgttgat
     4621 ccgtgtatct tcatttgttt tcccagctaa tccgatcgtt aagatttgac ggtttttggg
     4681 atcaagcatg acttgtaacg gcaaaccata acgtcgcaag ctctcttctt ctagagaacg
     4741 gaataattgt cctcttacac catccaatga aggacggaat tgatttaata atttttgttt
     4801 ctgttttttc ttcatatgtt ctctccttcg ctcataccct ttcgattata agaaaaaaac
     4861 agccaaatga aaaggagtaa tgcaaaaaaa cttgcgtata cgcaaattac aacagaaaaa
     4921 tttgcgtatt ttcccgtttt ttgaaagaaa aataaaaaat ggaacggatt atgtatatat
     4981 acataatccg ttccattctt tctatttttt cggtatcaag gcaaaaacag gtagttcagg
     5041 tgaataaatg acttcatgtt cgtgccaggg tttgaccatt agcgaatgat cgaaccgttt
     5101 tttcagttct tcgatgattt gatcatgtgt ccagtttctt gcaggaaaat gttggatttt
     5161 ctctgcatcg atcaaaaaga caaaatcgga atgaaaagtc tgggtaactt cttccgagag
     5221 agaattcaag cgttcgattg gatccataaa atcacctttc gacttaaaat tgggtagtat
     5281 ctgggtcttt tgctttgccg actacaccat ctaactgatc gatttgtttc atttcttctt
     5341 ctgttaattc aaaatcaaat aattcagtat tttctttgat tctgcttggt gtcactgact
     5401 taggaagagg aaggaattcg tgttgcagcg accaacgcaa agcaacttgt gcaatcgtct
     5461 tgtcatgtgc ttcagcgatt tgtttcattt caggcacatc gaagatttta ccagtgccta
     5521 atggactata tgcttcgagg atcatatcat gtttttttgc atactcaact acagttgtct
     5581 gtaattcgcc tggtgcaagg aaaacttgat tgaccatcgg cataattttt gctgtttttg
     5641 ctagtgtatc tagatggtgg ggtaagaaat tgctcacacc gattgctttg attttacctg
     5701 cttcatacag ttcttcaaat gctcgccatg tatctgcatt tgcttgttcc caattatcac
     5761 gaaaagcaac agggtttggc cagtggatca aaaacagatc taaataatct gtttggagct
     5821 tcttcaatga ttcttcaaaa gaagacatca caagttcata gctgtggttt gcattccata
     5881 attttgttgt caagaatatc tcttctctag gaattccact ttccttaatc gcttggccga
     5941 cgctttcttc attcttgtat ccttgggcag tatcgatatg gcgataacct gcagccagcg
     6001 cttctttaac agaggaaaca gcgacatccc catcaggtgt ttgccatgtg ccgaatccta
     6061 ctaccgggat ttcgtaacca tttgatagac gataagtgct tgttaatgaa ttcatacttt
     6121 cagctccttt tttattcgtt caactataag tgtagcgcat ttttcgaaaa aaagaatcca
     6181 ttttgtttct actcttgaaa tttttgttta aaagaaaatg attttgattc tggtattttt
     6241 gatttttttt ccgtaaacta agacagaagg gaatgagaaa atggatatct atttaaaaaa
     6301 agcaattctg catattattg acagagaaac tggttctcca gtcttttcac aaaaagagct
     6361 ggatctgaca aaagaatata ttcgtgattt tcttcaaaag aagatccaaa aaatctcttc
     6421 tgctcaaaca aaaactggac aactagcaga agaaagcatg atcgttgaat cacttaaaat
     6481 cgctcaagaa aattttgtgg aagcaagtga agcaatcgtt cagcggtggt tcgatatcta
     6541 ccaagaaagt gaagaagcac ctagtgcaga tgtatttgtt gtcttgtatg aattagatac
     6601 agtgatgtac ttatctttat tgaaagtcaa ttatcgtgag gcttacactc actttgttga
     6661 agcagacgaa gcagggatcg acaaccgctt gattttgaat cgagcaattc taggggcgaa
     6721 aacacaaaaa gcagatgaag cattcgctgt gaatttgact gattggacct acgaattaat
     6781 tgaaaaaaaa tatgagtttt ctggcaaaaa agagctttat ttttctggaa gagtgatcga
     6841 aagtgttcct gcaccttctt tagaagaaaa tgtaaaagtg atccaaaaag tagccaagca
     6901 tcttggaaaa aaatttgaga cagaagaatt tgatatcatg gctgatttga aagaagctgt
     6961 ctatgatacg atagaagaaa aaggtcggtt ggatcatgag atgattgcgg aaaaagtctt
     7021 caaggaaaac atcacggcaa aaatggcttt taaagaagaa gtacaagagc aaggatttgt
     7081 cccagaagca cctcctgtaa aagaagtaca agaaatttct gagaaaaaat ttggaaaaca
     7141 aaaattcaag ctatcaaacg ggatcgaact gatcgtacca gtagatgtat atcgcaaccc
     7201 agatttgatt gaatttgtga ataatccgga tggtacgata tccgtgatga tcaaaaatgt
     7261 ggatgaagta ttgaatcgtt tataaaaatg actagaatat taggtgttca agtaccagtg
     7321 atcagttaaa tgaattgtgt caagagaagg atcggaaaag acgaatataa caaaaaaaga
     7381 gcgtg
//
LOCUS       DACEUF010000105         7187 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.109, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000105 DACEUF010000000
VERSION     DACEUF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.109"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IXG20_002374"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IXG20_002374"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ1610545.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IXG20_002375"
     CDS             complement(1172..1867)
                     /locus_tag="IXG20_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ1610546.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IXG20_002376"
     CDS             complement(1880..2596)
                     /locus_tag="IXG20_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ1610547.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSILYK"
     gene            2926..3534
                     /locus_tag="IXG20_002377"
     CDS             2926..3534
                     /locus_tag="IXG20_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ1610548.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IXG20_002378"
     CDS             3859..4182
                     /locus_tag="IXG20_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1610549.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IXG20_002379"
     CDS             4364..5734
                     /locus_tag="IXG20_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ1610550.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            6081..>7187
                     /locus_tag="IXG20_002380"
     CDS             6081..>7187
                     /locus_tag="IXG20_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1610551.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKK"
BASE COUNT         2110 a         1445 c         1343 g         2289 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcgttatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttatata aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaacat actaagatta gaggggaaca tcattgacga atgatgttct cctctatttt
     5881 ctatttttag attatagtga agagtgaaag aaagttcgaa caaagccttg aggactacga
     5941 gtccctatca aaaaccgaac ttctttactg tcattttaga atcaggttta cgtatgttgg
     6001 ggccatgagc ccgtgtatag gaaattggaa tcgaaatgaa gcagtaaagt tctttcaaaa
     6061 cttaaaaagg aggaaaacaa atgccttaca ttcttgcatt agatgtttca atgggacaca
     6121 gctacagtgt cctatacaaa gacgacacct gcttatttga agacgagatt gaacacactc
     6181 aaagaggatt tcatgcatta ctcgaagaga tccagcactt gcctgagcgt ccattgattg
     6241 tttttgaagc aacggggatt tattccagac cagtcgaaaa gttctttcaa gatcatcact
     6301 tttcttactg tttgttaaac ccgttagagg cgaaaaaaca gatggaagaa agcaccttac
     6361 gcagttggaa aacagataaa tcggatgccc accggctcgc acagactcat cttaaaaatc
     6421 aacgtcagcc aaaagaagta caaagcaatt tttacttaga gatgcgcgat ttagcccgtt
     6481 tttatcaaga aattgagaag aaaatcactc gtctgcgcat ggatttacac aactgccttc
     6541 aattgacttt tccagagctt gaacagttct tttcaaacag gctgacccct tacgcattga
     6601 cattgatccg tctttttccc catccagatt ttgtattggc atctactcgg acaaaaataa
     6661 aaaataaact gatcaacgaa acacggaaaa aaatctccgc gaatcgtgcc gagcaaaagg
     6721 cagatcaaat catccactat gcacagtgtg cctatccagc tgttgaaaaa gacagtatcc
     6781 actgtcaaaa aacgatctat tacgccgaac ttctccaaga tttactcgaa caaaaaaaag
     6841 cactggcgac acaaatgatc aaaaaagccg aaggctctcc ttgctttttg ttgtaccaaa
     6901 cgttccctgg gattggcgca ctcacagccg ctttattgct cggtgaatta ggagatatca
     6961 cacgatttaa aacccataaa caattgaatg cctttgtcgg aatcgacatc cgacggtatc
     7021 attcaggtaa gtatactggc caagaccgca tcaataagcg tggtaatccc aaagcacgga
     7081 aaattatttt cttcaccatt cgaaatatga ttcgccagca acgcgcagct cctaatcata
     7141 ttgtcgatta ttactacaaa ttaaaaaagc aacctatccc taagaaa
//
LOCUS       DACEUF010000106         6892 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.110, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000106 DACEUF010000000
VERSION     DACEUF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.110"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..227
                     /locus_tag="IXG20_002381"
     CDS             <1..227
                     /locus_tag="IXG20_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAQ1610552.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..574
                     /locus_tag="IXG20_002382"
     CDS             227..574
                     /locus_tag="IXG20_002382"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610553.1"
                     /translation="MRKRTIKTQLAVSFLAIATLIIGSISLVALSLMNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKVLENNIYFSIEDEQGNMVWELTGK
                     DLKSAQEKLKKMH"
     gene            571..1620
                     /locus_tag="IXG20_002383"
     CDS             571..1620
                     /locus_tag="IXG20_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1610554.1"
                     /translation="MKVSEKNSVKLDETIEVKKKLINDGNEFGKVTFYYFGPFAYTEH
                     DALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTHKLTRGEYADKIP
                     QETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGV
                     WKITPERLQSCYDEIDRLTRLIGNIEIINKIEAKYDHLNKTEFNIYKLIESVIENFAS
                     KIESKNLHVEIQGDNINISADKDKMNQVVINLLNNAIKFTQKEGTIKFSISKNKDHVL
                     LIVEDNGIGIEKDQQLHIFDRFYMADPSRSRALGGQGIGLAIVKSVIEAHKGSITVKS
                     KLGLGTKFVIKLPFQ"
     gene            1635..1841
                     /locus_tag="IXG20_002384"
     CDS             1635..1841
                     /locus_tag="IXG20_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610555.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="IXG20_002385"
     CDS             1862..2458
                     /locus_tag="IXG20_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HAQ1610556.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
     gene            3410..4009
                     /locus_tag="IXG20_002386"
     CDS             3410..4009
                     /locus_tag="IXG20_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1610557.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            4058..4774
                     /locus_tag="IXG20_002387"
     CDS             4058..4774
                     /locus_tag="IXG20_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HAQ1610558.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            4788..5567
                     /locus_tag="IXG20_002388"
     CDS             4788..5567
                     /locus_tag="IXG20_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ1610559.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            5650..6006
                     /locus_tag="IXG20_002389"
     CDS             5650..6006
                     /locus_tag="IXG20_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1610560.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            5996..6262
                     /locus_tag="IXG20_002390"
     CDS             5996..6262
                     /locus_tag="IXG20_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1610561.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            6421..6699
                     /locus_tag="IXG20_002391"
     CDS             6421..6699
                     /locus_tag="IXG20_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610562.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(6696..>6892)
                     /locus_tag="IXG20_002392"
     CDS             complement(6696..>6892)
                     /locus_tag="IXG20_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1610563.1"
                     /translation="NNLIEQDHRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKE
                     GTLFGFSVCTEIRILLGIPA"
BASE COUNT         2534 a          959 c         1335 g         2064 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagagaga caactgaaaa
     2641 tttagtcata accagttagg cagagtgctt aactggtttt tgttcgcatt tattattctt
     2701 ttgatattgg ctgcttgccc ttcaatttta tttggtttct taatcaactc ttcatataac
     2761 cccaaatcat atattaatga ggtgcctcct attcctataa atctatctct tctttaatag
     2821 tatgagaaaa gtctactctt gtagatttaa attgtcaaca aaaaatttac agatgtaaat
     2881 gataagttga atagagaaag ataaattatt tttttggtat tcatataagg ttaattatgt
     2941 attatcacaa aatgattgtt gtcgaataac taaaagtgtc aaaacaatga tgaaatcatt
     3001 tagttcttat aaattagaca agagaaaata aatattccat atctacttta ttggatccat
     3061 aacatgacta aattctgaat actttttaaa gcttatcatt tggtaaaact gaatataagt
     3121 taattatata gactgaaaat tttgaatttt cagaatagaa taacttaaaa gtactgacaa
     3181 attttttaaa ggggcaatat taatgttctg agataatcaa atcttaccga aaatatcaaa
     3241 agtccaatgt gacacaattg gattttgtca cattcgttaa aatcgctcca tccttagagg
     3301 ctctaatagg aaatcacttt tcttttagcg ccttttttct atagcttgtt taaatgtgac
     3361 ttattatctg ttataatcgt attaagtaac atttgagagg tgtctaaaaa tgaaaagcgt
     3421 ggaaccgatt cgcgacaaaa aaaagatcga tgccatgaag gccattttgg cttccggaaa
     3481 atatgggcaa cgaaatttgg tgcttttttc gatcgggatc aatacagcct atcgaatctc
     3541 cgatttaaga cagttaaaat taagggatgt gctagaaatt tcccgtgggc gggtgatcgt
     3601 caaagaacgt ctggccatga aggaacaaaa aacggccaaa cacaattcgg tcttcatttc
     3661 gaacaaattg cgaaaagtga ttcttgatta tgttcaatcc gaatttcctg agcagttgca
     3721 agcacaagac ttcagcaagt atttgtttcc aagtcgaaaa ggagcagata cgcctttgac
     3781 ccgacaaagc ctttggcgga tcattcatga agcaggaact gcagttgggt taaaagaaat
     3841 tggtccccat tcgatgcgta agacctttgg ctatttttta tacaagcaag gaaccaaaac
     3901 agagatcatt cagtcgctgc taaaccattc ttcacaacgg gagaccttac gctatattgg
     3961 aatcacccag gaagacaaag atactgcagt aaagagtttg gatttatgac agaataatgc
     4021 cctatataat agaaggaaaa attcaagagg tgagaaaatg aacgaagaaa agcagcctaa
     4081 ctttcctgat aaatatcact tatctcgcaa agagtctgtg tatttattga agaaaaacat
     4141 tgtagaactg gtgtataatg cggggaaatt tgaagggtta aatacaacgt tgttacaaac
     4201 agaagggatt attaagtata atcgggccaa caacgtggct gtagatgatg tcctgaccgt
     4261 cgttaactta aaaagaggct ttgaaatgct tttaaatgac gtacaggagc ccctgttaga
     4321 gacaagtaag cggattaatc ggatcgttgc agcagaggat gcgctttttc ccggtgaaat
     4381 acgaaccggt ggggtagaat tatcgacgat tcaaggaaga tatgtgccac caatgttaac
     4441 tgaagacgag gtaaacaatc aatatggtga gataatgaat caagagatat cagatacgga
     4501 aaaagccctg cgactgtttc tattcatttc gaaaaatcaa atattctgga atggtaatgt
     4561 gggtacagcc ttgttgattg cgaataagat tatgtttagc aacggtttag gactattatc
     4621 aatacccgaa aatgtattta tgaaatttaa cgaactttta tcaaagtatt gcaacagtaa
     4681 tctattagtc aatgagtcaa atattatatc atttttatat gaacagtgta tctttggtat
     4741 taattatttg aaaataatcc aacatacaat ttaagaggta taagtaaatg atgactatag
     4801 acgaagcaaa acaattagca acttattttt cagttggttc agcgggatat attttagact
     4861 ttactaatgt aacgtggaac atatttacaa agaaaatcgt agggattgaa atacaaaatc
     4921 attatggggg ctcaaaggga gtatcctttc aaaagtttct gcttgacgat caatttgatg
     4981 attcgttaaa gaatcggtta atccaatctt taaaaggtat tatggatcga gataaaaaat
     5041 atgaatctac tgaaatgaat cccatagttc agttgctttt tgaggaagta tttaaagaag
     5101 atacttcaac tgcattaaga aaaatgaaat tttcaagcga gattgattta ctttatattc
     5161 ggcagctacc cattaggatt caaacagata ttaaaaacaa ggattttgac tctgttttga
     5221 caaaagcaaa tactttaata gatgaagttc taaaatttat tattgaaaat accgaaggac
     5281 cgagtgttaa tattaattta tttaagtcaa aagaattaag aaaagaagta tttggccgcc
     5341 tcaatattaa aactgaaaaa gatatggata atagaatcaa agcccttgtt ggagcactca
     5401 atacattatc agataaaata ctcgaaatga gaaatagtca aggagatgct catgcacatg
     5461 gctcaaatag aatagtaatt aaggaagaag aagcaattct tgttgcaaat tcttctatgg
     5521 ttttgtgcga gtatgtgttt actaagtata aaggattaaa tagataataa aatgcatatt
     5581 ttatagtata caattcgaat ttttatgtta tatactataa tataaaatca atatatgata
     5641 taattgggta tggaaaaaat atcttttgaa acatttaaaa gacccaatgg tcatgatgaa
     5701 tttttagaat ggttggaaac attaccaaaa aaagatagtg cgaagctttt acgtacaata
     5761 gaagaaacag aaaagaatgg gatgctagta gctcaacggt tgaaatgggt gaaaaaatta
     5821 gacactaatt tgtacgaatt aagaagtaag gtaggatcaa acatccaaag agccatctat
     5881 ttccatgtgg acaatggaag gtatgtcatt actcacggat ttactaaaaa aacggagaaa
     5941 acaccaataa ctgaactcaa gcatgcaaaa gaacttagag aggagtggtt cacaaatgat
     6001 gattaaaaaa ttgattgaag aaaagaaaaa agataaagat tttgcagaag catatgaccg
     6061 tgaaggaaga aaactcgaaa ctgcggttgc tctttatcat gctcgggagg aagcagggct
     6121 aactcaagcg gagcttgcac aaaaagcaca tacaactcaa tctactattg ctcgaattga
     6181 gagaggggat aacgtatctt tcgataaact tgatgatatc gccagggcat taggtaaaaa
     6241 gctggtagta gaatttgctt aaaagcgagt tgaaaatata tctataatag gctcctaact
     6301 aatcaattag tttagtgggc ctttttttac tagatagata aaggttgtac tatagtgtaa
     6361 aagacaataa attttatact aaaattactt taaagagaac tagagagaaa ggtacaataa
     6421 atgaaaacca taaatcttat aaaagtattt atagcatcac caggtgatat agcagcaaaa
     6481 aggaatgaag tagaagaaac catatataga tggaatatag aaaatacaga taatacaaaa
     6541 gtgattttga tgcctatacg atgggaaact aatgcgattc ctacttatgt aaataatagc
     6601 tctgggcaaa gtgttattaa taaacaaatt gtagaaaata gggttctgct gcaaagtttt
     6661 aaaaataaaa tcagtttttg tattatttct tcattttaag ctggaattcc caataatatc
     6721 ctgatttcag tgcagactga aaagccgaag agagtgcctt cttttcggct tttcttgtag
     6781 attcctcgaa tggtttccat ccctttaatt gtggtggaag ctgtacgtag actttgatac
     6841 aatttatgtc gacgttttat cgatcgatga tcttgttcta ttaaattgtt aa
//
LOCUS       DACEUF010000107         6821 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.111, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000107 DACEUF010000000
VERSION     DACEUF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.111"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            46..936
                     /locus_tag="IXG20_002393"
     CDS             46..936
                     /locus_tag="IXG20_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ1610564.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1009..1833)
                     /gene="nadE"
                     /locus_tag="IXG20_002394"
     CDS             complement(1009..1833)
                     /gene="nadE"
                     /locus_tag="IXG20_002394"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ1610565.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1845..3317)
                     /locus_tag="IXG20_002395"
     CDS             complement(1845..3317)
                     /locus_tag="IXG20_002395"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ1610566.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3954..4316)
                     /locus_tag="IXG20_002396"
     CDS             complement(3954..4316)
                     /locus_tag="IXG20_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1610567.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4539..5291
                     /locus_tag="IXG20_002397"
     CDS             4539..5291
                     /locus_tag="IXG20_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1610568.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5431..6354)
                     /locus_tag="IXG20_002398"
     CDS             complement(5431..6354)
                     /locus_tag="IXG20_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1610569.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6369..6641)
                     /locus_tag="IXG20_002399"
     CDS             complement(6369..6641)
                     /locus_tag="IXG20_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610570.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2089 a         1353 c         1164 g         2215 t
ORIGIN      
        1 caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata
       61 aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc
      121 cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc
      181 agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa
      241 aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat cagttttcca
      301 gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca
      361 aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg
      421 gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc
      481 agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg
      541 ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca
      601 gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt
      661 gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc
      721 ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca
      781 aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg
      841 aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac
      901 gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa
      961 acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa
     1021 atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc
     1081 gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt
     1141 atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc
     1201 agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc
     1261 ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt
     1321 gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc
     1381 tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa
     1441 atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt
     1501 gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc
     1561 ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt
     1621 tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc
     1681 ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc
     1741 tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc
     1801 taattcattg ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga
     1861 agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca
     1921 gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt
     1981 gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact
     2041 ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt
     2101 ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct
     2161 tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc
     2221 cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta
     2281 tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct
     2341 ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt
     2401 tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct
     2461 tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct
     2521 ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga
     2581 atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct
     2641 ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac
     2701 gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt
     2761 gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct
     2821 tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga
     2881 gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact
     2941 aattggcagt gcgctaaagg cccttccact tggattaatg gttcatttgc aaaaactagt
     3001 tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa
     3061 aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc
     3121 agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta
     3181 aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc
     3241 cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct
     3301 gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta
     3361 taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt
     3421 acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat
     3481 ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta
     3541 gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg
     3601 ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa
     3661 aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat
     3721 ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg
     3781 ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca
     3841 gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta
     3901 ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata
     3961 gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt
     4021 attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc
     4081 atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta
     4141 ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct
     4201 aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta
     4261 ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca
     4321 ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta
     4381 gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact
     4441 taatagtata acataatttc gtgagaaaaa aatacaattt tttcttgacc atctcgttac
     4501 gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa
     4561 tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc
     4621 taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc
     4681 gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg
     4741 tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc
     4801 tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt
     4861 acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt
     4921 tgaaagaaaa tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaagg
     4981 ttacagcatc caatgaaaaa tggatgaata tggataaaga aacgtatgaa gaaatgcaaa
     5041 ctgttgaaaa gcagatgtta tttgatctca ctctttactt aaaagaacca gaagatgaaa
     5101 gcttagcaga ccgtatcttc caagctcata agaagtggtt ctcttacagc tggcctgaat
     5161 atcagccaaa agcgcataaa ggactaggaa tgatgtacga agccgatgaa cggttcactg
     5221 catattatga cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa
     5281 aaaaggcgta atcttttaca tgatccaaca aaaaagtaca cgtaaaatga cctaataaca
     5341 aaacagcaga cacccaagtt agatttttat gctaactttt ggtgtctgct ccattctttc
     5401 acaagttttt tcatcctact ttaaatatat tcatcgttga ataaacgtat aaggtatttt
     5461 cactttatcc tttttttctg taagaaacaa ttgaaatgcc atttctccgc aagcttccag
     5521 atggtggtcg atggttggga aaccaagaag ctcgctgatc agcaaattat cgcgtccaat
     5581 caccactggt gtcttatctt gcagatagct ttgcaagatg cctgctgcaa ccatatccga
     5641 atttgtaaag acaccatcaa ctccaagctc tttaaaaaag aagcctgctt gttttccttt
     5701 ctctgcatct atacaatccc aaaaaacaag tgattcatca aaagatggaa aaagttgatg
     5761 acatagctgc aaagtgattt ttgagttata acttagcctg ccacttcgcc ccaacgtgat
     5821 gcccaattgc tttatacctt gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc
     5881 tcgatcaata aagacacagc ttgcctgttt tcccttgatt tcttcacaaa aaactagagg
     5941 tccatatttt tgatatgcca ataaatcatc tagtgtattg gcacgagacg taatgatcaa
     6001 tccatcgaaa gctttagctg caaattcttc aagatacctt cgctcttttt ctttatgata
     6061 gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa tgatcccact
     6121 gatcaactga tcaaaatacg gatgattggt ataaggcaga atcactccaa tgcttttcgt
     6181 ttttccataa cttaactgtc tggcatttgc attagggata taatcgactt ctttcatgac
     6241 cgcaagtatt tctttccttt tttcctcatc tacatatgga tgtccattga ttacacgtga
     6301 aatagtagaa actgaataac ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc
     6361 ttcgccacct atacaaaaag attttcttgt aaatagtcta ccacagctgg accgcattca
     6421 aaaaactgat acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata
     6481 tcttttgtca aattttcgat ttcattcatt ggtatttctt ttactatatt cctattcaac
     6541 ccttgaaaac tgtacaacgt taatacggat cccttgaact cgacaaacgc ataaaaataa
     6601 ttgttcgttg tgccatctgt tatctctaca taagtattca ttgcaagccc tccttttgat
     6661 actttgagta tagtgtatga aacgcgtctc atagcaagga gtttgttttg ccaaagaaaa
     6721 gttttattcc tctttcatta ttaccaaagt ctcttatttt aaattgaata cattataatg
     6781 atactgtaaa tatatcttta ttattagtga atggatattt a
//
LOCUS       DACEUF010000108         6812 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.112, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000108 DACEUF010000000
VERSION     DACEUF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.112"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(<1..152)
                     /locus_tag="IXG20_002400"
     CDS             complement(<1..152)
                     /locus_tag="IXG20_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1610571.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEIL"
     gene            complement(165..653)
                     /locus_tag="IXG20_002401"
     CDS             complement(165..653)
                     /locus_tag="IXG20_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ1610572.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(619..1290)
                     /locus_tag="IXG20_002402"
     CDS             complement(619..1290)
                     /locus_tag="IXG20_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ1610573.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            1428..2714
                     /locus_tag="IXG20_002403"
     CDS             1428..2714
                     /locus_tag="IXG20_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ1610574.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            2786..2980
                     /gene="ssrS"
                     /locus_tag="IXG20_002404"
     ncRNA           2786..2980
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IXG20_002404"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            3058..3342
                     /locus_tag="IXG20_002405"
     CDS             3058..3342
                     /locus_tag="IXG20_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610575.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            3415..3894
                     /locus_tag="IXG20_002406"
     CDS             3415..3894
                     /locus_tag="IXG20_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1610576.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            4117..4260
                     /locus_tag="IXG20_002407"
     CDS             4117..4260
                     /locus_tag="IXG20_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ1610577.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            4838..6421
                     /locus_tag="IXG20_002408"
     CDS             4838..6421
                     /locus_tag="IXG20_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1610578.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
BASE COUNT         2091 a         1404 c         1357 g         1960 t
ORIGIN      
        1 tctaaaatct caccatattg atccgactga aaatgttcaa catctaaaga atcttcaatc
       61 gcgacaccac tcgcccctgc ctccatcaaa atattagcaa ctgcttcgac tgcttcactt
      121 tcagtttcta cttttacttc attccatttc atatctgtca ctccttaata acttgggtac
      181 ggtaaataag tatctgcttc attttgctgt tttacttcta ctaattctgc tagttgctga
      241 ttatcccagt gaagctcgaa tacagcttcg tcatcgtctt catccaaaaa atcaagcaaa
      301 tcgctttgcc cttgagctaa tacttcattt agatattcca ctaaagaatc agctactgat
      361 tttgccaacc ctttttttcc ttcataaggg attactgcta gataattgcg ttcatcaaaa
      421 acagatttct gaggattaaa aagaagtacg ccgtcttcaa attcgataat tggttcttgc
      481 gaaacgatcc cttctgcatc atcaatctca aattgtgctt tattttctgc aaacaagcga
      541 acaacgatct cgatcgtatg attttttgta tcccaatcta aagctacatc atagtctgtc
      601 acttgttttt caattatttc atctaaatat gttaatagat ccttcttttt cattttcttt
      661 cctccaaccc caatctttct cgatttggtt ttttctttgt aaagagatat aaggatttcc
      721 cgacacaaca aaacgcaacg gcttttctgt ccatacacct ttgtttggga taccgattct
      781 aggcaaagca ttgatctttt ttggtgtctt ttttttctca aaaactaagc gtaacgaact
      841 atctgctata gactgaccat aaagttcttg aggaattgct aatgcttcca ctaattttcc
      901 cggaccgttg ctgatatctg gaccagtttt tccgccacga tttttactca tttgatcaat
      961 cattgcggcg ggttcgattg cacgtatcat tactccttga ggaatccctt ctggctgagt
     1021 aatgatgttc aaaatccgat gagtatgcat cgtgtaaagg tagatcgtac ctggtttttc
     1081 atacattgcc cgcacacgcg gtgttcttct taaaccatag ctatgggcgg cctcgtcttc
     1141 tggacctagg tatgcttccg cgtctacaat atatccagct aatcttcctt ctggtgtgtc
     1201 atgctccaaa tacatcccca ataaatcccg tgccacttct ggcgtcgttt tattccaaaa
     1261 aaattcatct atcttttgtt tatccgtcat ttttctcacc tactttatca tacacaatat
     1321 tagttggata agctatgaaa acaaaacaac agaaggctgt tctttctagg catcttgcta
     1381 aatatgatat aattaaggga cgtaaaaaca agaaggagga aaatcctatg caacagccat
     1441 tggcttatcg catgagacct cgaacgatcg atgaagtcgt tggtcagcag catctagtcg
     1501 gggaaggaaa aatcatacgc cgcatggtcg atgctcgcat gctctcttcc atgattctat
     1561 atggacctcc tggtactgga aaaacgagta tcgcaagtgc gatcgccggc tctactaatt
     1621 atgcatttcg catgctaaac gccgctacag attcaaaaaa ggatctacaa gttgtagcgg
     1681 aagaagcaaa aatgagtggt acagtgatcc tcttattgga tgaagtacac cgattagata
     1741 aaacaaaaca ggattttctg cttcctcatt tagaaagtgg ccgaatcatc ttgatcggag
     1801 cgactactga gaatccttat attaccatca atccagcgat ccgcagccgt acacagatct
     1861 ttgaagtcaa accattaaca gaacaagaca tccagttggc agtcgaacat gctttaaggg
     1921 ataaagaacg cggccttgga caacaagcaa tccagttaga cgaagatgct cttcttcacc
     1981 tttcgcgtgc aacgaacggc gatttgcgga gtgcattgaa tgggctggaa ctggctacac
     2041 tatcgactcc ttcagataaa gaaggaagga ttcatcttac actgtcgatc attgaagaat
     2101 gtgtccaaag aaaagctctt acccatgaca aaaatggaga tgctcattat gacgttattt
     2161 cggcttttca aaaatcgatt cgtggaagtg atgtggatgc tgcacttcat tatctcgccc
     2221 gtttagttga ggcaggtgat ctggcaagta tttgccggcg tctgatggtt atcggttatg
     2281 aagatatagg tttaggtaat cctgctgctg cggctcgtac agtgaacgct gtattagctg
     2341 ctgaaaaatt gggattgcct gaggcacgta tcccactagc agacgtcgtc gtcgatcttt
     2401 gcttgtcgcc aaaatccaat tctgcctaca tggcattaga tgcagcactt gctgatattc
     2461 gagagggcaa agctggagat gttcctgatc atttgagaga tagccattac aaaggtgcga
     2521 aatcactcaa ccgtggcgtg ggttatcagt atcctcatca ttttgatcag gcttgggtca
     2581 atcagcaata tttaccagat aaactgaaga atgctcagta ttatcagcca aaagatacag
     2641 ggaaatatga acaggctctg ggtcaacagt attaccgtat aaaagagtgg aaaaagcatc
     2701 ctccaaaaaa ctagacaaga aaacggttct gtttctgcga gctctctcat tgctcttttt
     2761 aaattcatat gctactatag aggtgggaaa tctgtgatgt tcgaattgtc ctctttgtgt
     2821 gttgaccgaa catttttatt gatatcttgg gacccgtctt gtgtcactgc tggtaacatg
     2881 ccttatcatt tggtagttcg aagctacgac catgggaccc acctgcttga attcgcggga
     2941 tcaatactac aggacaaaca acggcattaa cggagattac catcttttaa agaataaagc
     3001 aatttgctct tggcaaattg ctttttctat ttgttgctta ttttggagga tttgttcatg
     3061 attaggattt taatgattat cgccacttta gtattattat tcgttagcta ttatttattt
     3121 aataaacagg atatcttttt tgtattgatc aacaaaaatg ataaaaatca aggattccta
     3181 caattttacg gagctgcata tgctgtttta ggcgtaatgg gtattttaac agcttttttc
     3241 aatcaacgct ttattgcctt ggtcttttta ctgatcgtaa ttattgtttc tgccaccttc
     3301 agtatccgat ttgcaaaaaa aattgctgaa ccaaagcagt aaataattct ctttttccct
     3361 aaagtgttct acaataaaag agtacaatac gattgacttg aggagtgaat gcttatgtta
     3421 caacaataca aaaaaatcat ggttgccgta gatggttctg atgaagctga attagctttt
     3481 aaaaaggcgg tcaatgtagc tatccgaaat aacggagaac ttctattagc acatgtcatt
     3541 gacacacgtt ctttccaaac cgtttcatcc tttgatggaa tgcttgcaga acaagctact
     3601 gaaatggcaa agcaaacatt ggcagattat gaaagcaatg cgaagaaagc tggcttgaat
     3661 aatgtaacct ctgtcgtaga atatggctca ccaaaacaaa ttattgccag agaaatccca
     3721 gaagataatc aagtagattt gattatgtta ggagccacag gattgaatgc tgtagaacgt
     3781 ttatttattg gttctgtttc tgaatatgtt attagaaacg cagcttgcga tgtgcttgtt
     3841 gtccgaactg atttagaaaa ccaattgcca gctaaagacc acaccaaagc ttaacacgta
     3901 gacgacctgc caactatcgg caggtttttt tgcacaaaaa accgtgataa aaacaaaaga
     3961 cgcggcaagc actcatcggt ctttataatg tttaaaataa agggaaaaca gtcaatgagt
     4021 tcaagtaaaa taaaaaaaag tgaacaccag ggcgattagt cgccctggtg tggtttaaaa
     4081 cgacctaagc atgccaaagc ttattggttc gtcaatgtgg aagtttccga tgaatctgat
     4141 acgaaaaaat atgaagtatc gatggcggat accgatttta tcgccaaact aaaattttat
     4201 ttcgggttcg ggtaccgcga gaaggcaagt gctacattcg gtaaatcatt caagaattga
     4261 tgaaaggcta ccgcttgcca ttgcaaccac aacgatactc ctgaacgcga tgttggtgag
     4321 atttccaaag agaagagctg tttaatgcaa cggtattaag caaatggcaa aaggaagtca
     4381 tgtagacaaa gttttttatc aaacaccata tgatttcgcc tgaacgctgg tttagctcag
     4441 aatacaatga tgtggtccac gagacattac cctcagtcag ataaaaaaag ctctctgaca
     4501 gaattacaca tcgataaata ttaagtaaat gtcagcgcta gttgaacttt ctgacacttt
     4561 caggtacact ggaaaagaca actacacgaa ggagggaaaa tactatgatg cgcatgacta
     4621 aaataacaag taatgaaaat taccaacgat accatatcgc atttagccat gcaattggtt
     4681 ttacagttgc tgttgacggg acaagtttgt ccaaaacagt tgaattcacg taaaatcaat
     4741 ggccatctca aggtattttt ctcatgataa tctgagttta aataacacag gccatggata
     4801 accatggcct ttttgagtgg tctcaaggag agagatcatg atcatacaag caatttctat
     4861 taaaaaaaca atcggcggga atccattatt tgaggattta tcctgtcaaa ttgaagctgg
     4921 tgaaaaaatt gccttagtcg gtatcaacgg aactggcaaa tcaacctttt tacaaatctt
     4981 gaccggcaga gaaggtgtcg acagcggtgt tatcagtcgc aaaaaaggct taaagatcgg
     5041 gacggtcgaa caagaactta cagtttccga agcgaccgtc aaccactact tgctccattc
     5101 tgccgctgag atacaggacc tcaaacagca aatgagtcgc tacgaagcat tgatgacaga
     5161 accagataca aacttagaga agtgtctggc ccgttacggt gagctgcagc atcgctttga
     5221 agaactaggc ggttacgtat tagaagaccg gattgcagcg atcttgcaag gcttagggat
     5281 tccacaccgt caggaagcac ggttaacaga acttagcgga ggcgaacgag ttaaagtcgc
     5341 tttagccaaa attttagcag ccgatgccga tttactttta ctcgatgaac cgaccaatca
     5401 tttagatctt aacagtattc gctggttgga aaactattta cagaatacaa aaaaatcggt
     5461 tctggtcgtt tctcatgatc ggcaatttct cgatcaagtc accacaaaaa catgggaact
     5521 ggaagaaggt gcattgattg agtatcccgg aaactacagc cgttttcgag tcttgaaaga
     5581 agctcgcttg gctgagctga caaaaaatta cgaactgcag caaaaagaag tccagcggtt
     5641 gaaagtcatg attcgtcgtt ttcgtcagtg ggcacatgaa ggagacaatg agtccttctt
     5701 caaaaaagcc aaagaactgg aacgccgcct ggcgaaactg acactcgtca aaccgccacc
     5761 tcccccgaaa aatcggctgc aaagcttgtc taatggtgga aaatctggca aagaagtttt
     5821 catcattcaa aacttgcacc agcagtacgc tgatcaagta ctattcaaag acagcagttt
     5881 tgcagtctat cgtggagatc atttagccat catcggcgac aatggtgcag gaaaatcaac
     5941 cttactcaaa ttattactcg gtgaggaaaa gccttacggt ggcaccatca aattaggtag
     6001 tagcttgcaa atcggttatt tgccgcagca gctgcagttt gccgatcccg atagccgttt
     6061 gctggcttac gccatcacat tgactggaaa cgaagaagtc gctcgacggc aattagccaa
     6121 aagtggcttt tatcaaagcg atgtgggtaa acggatcaaa gatttatccg gtggtgaaaa
     6181 aatccgtttg gcgttgctga aactcttttt agaaaaaatc aacgtcctga ttttggatga
     6241 accaaccaat cacctcgaca gctatgctcg agaagaaatt gaagaaatgc tgcaagacta
     6301 tcaaggaacg ctcctagcag tcagccatga ccgctacttt ttgcagcaac acttccaaga
     6361 agcactcgtt attgatcagg agcagatcag ccgccagccg ctcggccaac tgattgacta
     6421 ggacgttttt tcgcaacaaa aaacttgatt ttcatacctg tgctttcacg attcaggcaa
     6481 agtacaggtg gaaaatcaag tttttattat tcgatcaatg aatgtttcgg atcggttttc
     6541 tagctaaaca taaagcgaag aataaagtcg acagcccacg aggctgatct aaaaatgttt
     6601 tttcttttta catatgtaat gtcccttata aagatgaatg aaaataaaat attgggattt
     6661 tcctgaaaat caataaataa aatgccagtc ttttcccttt tgaccacttt tccaagcaaa
     6721 attccctaag aagatcctgt aaaaaagaag ggaaattgaa gttaaattac cattttgcgg
     6781 tcttctccgc aaaaaaaaca catgcaagaa tt
//
LOCUS       DACEUF010000109         6065 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.113, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000109 DACEUF010000000
VERSION     DACEUF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.113"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            832..1566
                     /locus_tag="IXG20_002409"
     CDS             832..1566
                     /locus_tag="IXG20_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1610579.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="IXG20_002410"
     CDS             1582..2751
                     /locus_tag="IXG20_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1610580.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="IXG20_002411"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="IXG20_002411"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1610581.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="IXG20_002412"
     CDS             3841..4767
                     /locus_tag="IXG20_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1610582.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="IXG20_002413"
     CDS             4786..6036
                     /locus_tag="IXG20_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1610583.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2026 a          997 c         1245 g         1797 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agtta
//
LOCUS       DACEUF010000110         6062 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.114, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000110 DACEUF010000000
VERSION     DACEUF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.114"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(<1..182)
                     /locus_tag="IXG20_002414"
     CDS             complement(<1..182)
                     /locus_tag="IXG20_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1610584.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="IXG20_002415"
     CDS             517..951
                     /locus_tag="IXG20_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1610585.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="IXG20_002416"
     CDS             974..1294
                     /locus_tag="IXG20_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610586.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="IXG20_002417"
     CDS             1435..1626
                     /locus_tag="IXG20_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610587.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="IXG20_002418"
     CDS             complement(1697..1954)
                     /locus_tag="IXG20_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ1610588.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="IXG20_002419"
     CDS             2112..2375
                     /locus_tag="IXG20_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610589.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="IXG20_002420"
     CDS             2399..2587
                     /locus_tag="IXG20_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610590.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="IXG20_002421"
     CDS             complement(2670..2855)
                     /locus_tag="IXG20_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610591.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="IXG20_002422"
     CDS             3070..3441
                     /locus_tag="IXG20_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1610592.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="IXG20_002423"
     tRNA            complement(3591..3663)
                     /locus_tag="IXG20_002423"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="IXG20_002424"
     CDS             complement(3709..4137)
                     /locus_tag="IXG20_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610593.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="IXG20_002425"
     CDS             complement(4197..4955)
                     /locus_tag="IXG20_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ1610594.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="IXG20_002426"
     CDS             5250..5447
                     /locus_tag="IXG20_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610595.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="IXG20_002427"
     CDS             5553..6035
                     /locus_tag="IXG20_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610596.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
BASE COUNT         2096 a          921 c         1201 g         1844 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gt
//
LOCUS       DACEUF010000111         5844 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.117, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000111 DACEUF010000000
VERSION     DACEUF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.117"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            49..309
                     /locus_tag="IXG20_002428"
                     /pseudo
     CDS             49..309
                     /locus_tag="IXG20_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            472..1137
                     /locus_tag="IXG20_002429"
                     /pseudo
     CDS             472..1137
                     /locus_tag="IXG20_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            1173..1418
                     /locus_tag="IXG20_002430"
     CDS             1173..1418
                     /locus_tag="IXG20_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1610597.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1521..2390
                     /locus_tag="IXG20_002431"
     CDS             1521..2390
                     /locus_tag="IXG20_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1610598.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2371..3105
                     /locus_tag="IXG20_002432"
     CDS             2371..3105
                     /locus_tag="IXG20_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1610599.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            3138..4046
                     /locus_tag="IXG20_002433"
     CDS             3138..4046
                     /locus_tag="IXG20_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ1610600.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4043..4585
                     /gene="sat4"
                     /locus_tag="IXG20_002434"
     CDS             4043..4585
                     /gene="sat4"
                     /locus_tag="IXG20_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1610601.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            4678..5472
                     /locus_tag="IXG20_002435"
                     /note="aph(3')-IIIa"
     CDS             4678..5472
                     /locus_tag="IXG20_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1610602.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(5580..5768)
                     /locus_tag="IXG20_002436"
                     /pseudo
     CDS             complement(5580..5768)
                     /locus_tag="IXG20_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5748..>5844
                     /locus_tag="IXG20_002437"
     CDS             5748..>5844
                     /locus_tag="IXG20_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1610603.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNIS"
BASE COUNT         2021 a          867 c         1269 g         1687 t
ORIGIN      
        1 aatttgtatc gataaggggt acaaattccc actaagcgct cgggacccct tgtaggaaaa
       61 tgtcctaagt gtggcaacaa tattgtatta aaaaaatcgt tttatggttg ttcaaattat
      121 cctgaatgta agtttacttt agctgaacat tttagaaaga aaaaactaac caaaacgaat
      181 gtaaaagaat tactggaggg aaaagaaacc ctggtaaaag gaatcaaaaa caaagagaaa
      241 aagccctaca atgccgttgt aaaaattggg gaaaagggat atattgattt tatctctttt
      301 tcaaaataaa catggggctg acccaaaagt agctcctata tgaaaaatag aggtctttcc
      361 atttagatag tagtggaaag acctctattt atcttttgaa agtgaataca aaaataaagc
      421 acactctgct ataatatagg tgattaatcc atttgagaga ggtgctttat catgtatcaa
      481 aattatacca taaaccaact ttgtttgcct atagatcttg aaattaaatt agaagaaaat
      541 gattttgctc atgccattgt gcagtttgtt gactctattc ccgatgaagt ctttcttcct
      601 tattatcaat ctatgggtag accccaatat catcctcgta tgctactttc cattattctt
      661 tgtgcctata ttcaaggggt ttattcgggc cgccaaatac aaaatatgct gatagatagt
      721 attcggatgc gctacttatc tcaagagcaa ttccctaact ttcgtactat taatcgtttt
      781 cgcgttcatc cgattatgaa cgatattcta gatcattcat ttgttcaatt tcgtgagcta
      841 ctggttcaaa gtggcttaat cagtggcagt gctctataca ttgatggtac aaaaattgaa
      901 gcggatgcga ataaatactc tttcgtttgg aaaaaaagta ttttaaagta taaggatagc
      961 cttgatcaaa aagcccttga aaactaccaa caaatggtgg aaacaaaaat cttacctgag
     1021 ttaatagatg atttaaagga tgaactttct atagaagaga ttgaaaggat tcgtcaatct
     1081 ttggaagaaa aggaaaatca attaattgaa gcgattgacc aaaccgaaac agtagaaggg
     1141 ggctgaccca ttattaataa ggagtggaca acatgattga tgtaaatgag ctattggaag
     1201 aagctattag agaaactgaa aatctaaatg atggcgaagt ttttcttgtt aaagatttat
     1261 tcaagggata tgtggggaat aggataccca gaaaggatcg acttctgctt ggaacgttat
     1321 ttttaaacca tgttagcaaa atgaatggta atttaaaggc tattgaaaag acttcatcta
     1381 atcagcagag gtataaaaag acaattgata agttttaact atcttttgtg aaattaaaaa
     1441 aattataaat atgttgagta gaagcaaaag atttggcttg gaatttttac agcagtgaat
     1501 cgataaaagg agcatgcttg atggtagata atattattaa atcagtagca gagaaattat
     1561 cctctctgtc ttatatagaa ggtattgttt taggtggttc acgtgcaagg ggcacccata
     1621 cagaggattc ggatatagat atcggcatct attacaattc agaatcattt gacataaata
     1681 ctattaatca attcgctaca aagctggatg atgagcatag aaataacctt gttgtacctc
     1741 ccggagcatg gggtgattgg attaatggcg gcggatggtt agtcataaac gggtatcatg
     1801 tggatttaat tttacgtgat attaaacgtg tggaacaaat aatgaaagat acagagcacg
     1861 gaattgttac tgccaattat cagactgggc atccccatgg ttatattagt gcaatgtatc
     1921 gaggagaatt agcgattagc aaaatactat atgctaagaa tgaaagctta tgcgaattaa
     1981 aaaaacaggc agaaacttat cccaatgctt tgcagaaaag tttagttaac ttttttatgt
     2041 ttgaagcagg gttctcttta atgtttgtaa aagcaaattc gggaacagac gataaatatt
     2101 atattgcggg tcatgttttt cgtatagttt catgtttaaa tcaagtgtta tttgcatgta
     2161 ataatgctta ttgtatcaac gaaaagaaag ctataaaact gcttgaaact tttgaacata
     2221 aacctgaaaa atataccgag aaggtaaatc atatttttga agtactcggt atctcacttt
     2281 ttgaatgcta cgacatgacc gagaagcttt ataatgaagt gaatgaaatt gtatcggaga
     2341 taaataactt tttaaacgag gagagttcag atgaaagaaa acaaatatga tgataatata
     2401 ttttttcaaa aatacagtca aatgagtcgc tcgcagaaag gactggctgg tgcgggagaa
     2461 tgggagactt tgaaaaagat gctacctgat tttaagggta agcgtgtgct tgatttagga
     2521 tgcggctatg gatggcactg tatatatgcg atggaaaacg gtgcttcctc tgtagtaggt
     2581 gttgatattt ctcataaaat gctcgaagta gcaaaaggaa aaacccattt tccacagatt
     2641 gaatatgaat gctgtgccat agaagatgtg gatttcccag aggagagctt tgatgtaata
     2701 ctaagttcgc ttgcgtttca ttatgtagca gactatgaga atttaataaa aaagatatat
     2761 aggatgctga aggctggtgg caatttagtt tttacagttg aacatcctgt ttttactgct
     2821 catggaacac aagactggta ttataacgaa aaaggagaaa tactgcattt cccggtggac
     2881 aattattatt atgagggcaa acggacagct atgtttttgg aagaaaaggt tacaaaatat
     2941 catagaacac tgaccacata tctaaataca ctgctttcaa atagttttat aataaatcag
     3001 attgtggagc cacagccgcc agagaacatg atggatattc cggggatggc ggatgaaatg
     3061 cgacgcccaa tgatgctgat tgtatcggca aaaaagaaga tgtaataata tagaaaaaat
     3121 aaacgaggag tatgtaaatg agatcagaaa aagaaatgat ggatttagta ctttctttag
     3181 cagaacagga tgaacgtatt cgaattgtga cccttgaggg gtcacgcgca aatattaata
     3241 tacctaaaga tgaatttcag gattatgata ttacatattt tgtaagtgat atagaaccgt
     3301 ttatatctaa tgatgactgg cttaatcaat ttgggaatat aataatgatg caaaagccgg
     3361 aggatatgga attattccca cctgaagaaa agggattttc ctatcttatg ctatttgatg
     3421 attacaataa aattgatctt accttattgc ccttggaaga gttagataat tacctaaagg
     3481 gcgataaatt aataaaggtt ctaattgata aagattgtag aattaaaagg gacatagttc
     3541 cgactgatat agattatcat gtaagaaagc caagcgcaag ggagtatgat gattgctgca
     3601 atgaattttg gaatgtaaca ccttatgtta ttaaaggatt gtgccgtaag gaaattttat
     3661 ttgctattga tcattttaat cagattgttc gccatgagct gctgagaatg atatcatgga
     3721 aggtcggcat cgaaacaggc tttaaattaa gtgtaggcaa gaactataag tttattgaaa
     3781 ggtatatatc cgaggatttg tgggagaaac ttttgtccac ctaccggatg gattcctatg
     3841 aaaacatatg ggaagcatta tttctatgcc atcaattgtt cagggcggta tccggtgagg
     3901 tggcggaaag gcttcattat gcctatccgg agtatgatag gaatataaca aaatatacca
     3961 gggacatgta taaaaaatac actggtaaaa ccggctgcct ggatagcaca tatgccgctg
     4021 atatagaaga gaggcgggaa cagtgattac agaaatgaaa gcagggcacc tgaaagatat
     4081 cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt atgaaaacga
     4141 gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct accaagacga
     4201 agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata ctgataagat
     4261 aatatatctt tactaccaag acgataaatg cgtcggaaaa gttaaactgc gaaaaaattg
     4321 gaaccggtac gcttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     4381 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     4441 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     4501 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     4561 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt
     4621 ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg
     4681 gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa
     4741 gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta
     4801 tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag
     4861 gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg
     4921 catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag
     4981 tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc
     5041 tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag ccgattagcc
     5101 gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa
     5161 gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa
     5221 gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc
     5281 aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt
     5341 gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt
     5401 tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat
     5461 gaattgtttt agtacctaga tttagatgtc taaaaagctt taactacaag ctttttagac
     5521 atctaatctt ttctgaagta catccgcaac tgtccatact ctgatgtttt atatcttttc
     5581 taaaagttcg ctagatagag ttctatatta gagatgtaaa gagttttggt gaagaggttt
     5641 gtgagtatgg cttttataac cagggtgcaa tgagaagcac taacagcagc tagcttaaga
     5701 acgcatagga tatacttttt atggaagtca cagaggagga ataaaatttg aacaggatta
     5761 acagagtaac ctctattctt attcagctgc aatcaaaaaa aataattcct gccaaagaaa
     5821 ttgcacagcg atttaatata agct
//
LOCUS       DACEUF010000112         5770 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.118, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000112 DACEUF010000000
VERSION     DACEUF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.118"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(1015..1305)
                     /locus_tag="IXG20_002438"
     CDS             complement(1015..1305)
                     /locus_tag="IXG20_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610604.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1647..1943
                     /locus_tag="IXG20_002439"
     CDS             1647..1943
                     /locus_tag="IXG20_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610605.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1950..3902
                     /locus_tag="IXG20_002440"
     CDS             1950..3902
                     /locus_tag="IXG20_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ1610606.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3974..4471)
                     /gene="dfrG"
                     /locus_tag="IXG20_002441"
     CDS             complement(3974..4471)
                     /gene="dfrG"
                     /locus_tag="IXG20_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1610607.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4798..5490)
                     /locus_tag="IXG20_002442"
     CDS             complement(4798..5490)
                     /locus_tag="IXG20_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1610608.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1894 a          997 c          917 g         1962 t
ORIGIN      
        1 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
       61 gcataaagtg tttagaaaag aacattaaga atagttctat aataattctt gaactccatg
      121 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      181 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      241 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      301 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      361 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      421 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      481 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      541 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      601 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      661 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      721 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
      781 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
      841 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
      901 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
      961 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1021 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1081 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1141 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1201 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1261 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1321 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1381 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1441 actaaaaaaa agaggcagca gtatagattt aagtagttca gttttggagt acaaaaagtg
     1501 aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt
     1561 taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat
     1621 gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta
     1681 ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta
     1741 agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat
     1801 atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata
     1861 agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg
     1921 gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg
     1981 aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta
     2041 aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct
     2101 tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca
     2161 atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac
     2221 caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca
     2281 atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt
     2341 ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc
     2401 cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc
     2461 cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg
     2521 cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac
     2581 aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc
     2641 gtacttgtga tattttagga attggagttg gaacctacta tcataagctg gagtggctgt
     2701 ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat
     2761 ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga
     2821 aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag
     2881 ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga
     2941 atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata
     3001 cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta
     3061 acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta
     3121 gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa
     3181 ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta
     3241 gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta
     3301 tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa
     3361 aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt
     3421 tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta
     3481 gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca
     3541 ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa
     3601 acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac
     3661 agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga
     3721 agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa
     3781 gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg
     3841 ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat
     3901 gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca
     3961 attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat
     4021 acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat
     4081 tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt
     4141 tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt
     4201 cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa
     4261 aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga
     4321 ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat
     4381 attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc actctattct
     4441 tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta actaatttac
     4501 catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg ttagttgaat
     4561 aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa ttttcattca
     4621 attatcattt tcaattgcat tttataaatc attttgtatg taatttggta tgtaagttaa
     4681 aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca aaactgaact
     4741 acttagtata gattactgtt gcttctttac taatttaggt ttacttgtta agaaagatta
     4801 tttacgcaaa tgttcttcaa caatttgctg agatttatca gctgtatctt ccacttcatc
     4861 ttttgctttt tctaactttt gaatatattg tttcaagctt gagttttcag cagttgcttc
     4921 gtctaatttc ttttgtgtat ctgttaactg agtttctaca tttgtttttt cttgtgtcag
     4981 tgttgcgatt tgttgattca aaccatttac agtattttgt aattctgaaa gttttgcttg
     5041 tgcttcttgt agtcttgctg tccaatctgc attcagatga ttgatttcat catttttttg
     5101 gttgatctca tctagtttga cattgatttc attttgtttg ctttgtagtt gtgcatcctt
     5161 gctgactaat tgactttcta agttttgttt ttctgctgcc gattgatttt tttggttttc
     5221 taattcattt acttttgttt ggtattcttg gacatctgcc tttgaatctt gcaatgtctg
     5281 ttctttttgt gctagtcgag cggtaagatc tgtaatcgct tgctctttat ctgtcaatgt
     5341 atgatcaagc tgtgtcagat tctcattgat tgtatcaata tctgtctctc caccccacaa
     5401 aataatttca tttgcgtacc gcgtgcctgt gtagccaatt agacatagaa atgcaacgac
     5461 aatcccacca gtaataaatt tctttttcat ttgcatagct ccttaatcca atacttttac
     5521 atgctaaaag taagtgatct caaacacaat gtcaagcgtt tttaaattac atatgtaatt
     5581 ttaatataaa tactatcaaa tactcattta atcctccttt tttatctatt taaaaaatct
     5641 tttttataga gaaaaatgca ggttaatact ttcttaaata aatatttatg gtgaacaaaa
     5701 acaaaaaagc aagttttatt aaacgtaacc cttttcattt catagattac tgtatttatt
     5761 acaaaaaacg
//
LOCUS       DACEUF010000113         5745 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.119, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000113 DACEUF010000000
VERSION     DACEUF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.119"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(131..802)
                     /locus_tag="IXG20_002443"
     CDS             complement(131..802)
                     /locus_tag="IXG20_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610609.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(795..1289)
                     /locus_tag="IXG20_002444"
     CDS             complement(795..1289)
                     /locus_tag="IXG20_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1610610.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(1309..2529)
                     /locus_tag="IXG20_002445"
     CDS             complement(1309..2529)
                     /locus_tag="IXG20_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1610611.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            2676..4511
                     /gene="lepA"
                     /locus_tag="IXG20_002446"
     CDS             2676..4511
                     /gene="lepA"
                     /locus_tag="IXG20_002446"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ1610612.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(4605..5732)
                     /locus_tag="IXG20_002447"
     CDS             complement(4605..5732)
                     /locus_tag="IXG20_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1610613.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         1759 a         1154 c         1093 g         1739 t
ORIGIN      
        1 agaaaaaagg ggtctgacac aactagtgtc agacctcctt ttttcaaata aacagtgttc
       61 cagaagtagt ttcttcgaaa aataagccga aatttcttga agctgcccgc ttctgtcaca
      121 acctctttta ttaggtattt ttcacttttt ctaatttata actttcaaag taacgctcat
      181 cttttttact ttgagaccat gcaaacaatc ccattaaggc agcacctatc gtatttgtca
      241 gcaagtcgcc catcgtatcc atcaatgctg ctcgtccgac aaacagtgtg ccatcagaag
      301 cagcaaaacg ttgaagattc atgcctaaga actggtcgca taagaattcc cagaactccc
      361 aaaacacgcc gcaaagtcca gcaaacgcaa agccgaataa gaggaacaac caaggactgg
      421 tttttgatat ctcagcatct ttcatcaagt aaccaatcaa tccatagcca agagctgtca
      481 ggaccattgg actcaccgca tgtaagattt tgtcccaaaa agagatgata ctgatcaaat
      541 gcatccctgt gcctaaaaag acagagatga acaagaaaaa ccagtaaaac aaaacgattg
      601 ctgctggtat tttaagtttg aaaactttca aaatgacttg tggcatgaaa atcaatgcaa
      661 tccctaataa agattccacg ataaatgtga ttccttgaga agtcgaatat ttttgtgtga
      721 taaattcata aaaattatag cagacaacga atacaccaaa agcaagtaaa gctatttgca
      781 cctttttgat ttctttaatc actgaaagtc cctccatcta aaaatctttt tacttctgaa
      841 agcaactcaa tttctgaaga aaccactttt ccgttcaatt tgatcaaacc tattgtatat
      901 aagttcaggt agtgaaattg attttcagct gtttcttgta gtgcagcgat ttttttcggg
      961 tgggttccgc cttgctgtcg agtatcagta tataagccga tcactgggat gcctttcgca
     1021 taagccacac cgatttccga tgctaccccg gcatcgatcg tcagcccatc tagtaaagca
     1081 atcattaaat cactagccaa cactttttct gtgtcagcaa gagcaatcat tcgactgtct
     1141 gcataagcag ttttgtcatt gattgcttca ttttcttgag gaagatagat cgaaagctca
     1201 gaagagagct ctctgatttc tttaactaac tgttggttat ataacaaatc acttttagca
     1261 aacattggtg cggcaaaata aattttcatc taaacatcat cctttttatc aaaaataatc
     1321 gctaaagtaa gcttccatgt ttggaatgat acgctctagt gacttcagtg tttcttcatc
     1381 tgtttctaac cgttcgattc ttgccaaata agaagcacgg cgataattca tcatcaagca
     1441 agtcaatgtt tggatatcca gtcgaacgga ttttcctata ggttcattta agacacggac
     1501 ttgtccctgt tcatcccatg ttaaagcgaa gacaccatta ttccaaggag cgacaggatc
     1561 ttcaatgatg aaatgaaaag catcagctgt tccaacaaac ggaaaccgtt gaaggaattc
     1621 ttttacatca acgatccgag ccataaaata tggttcgatc tgctctttga tctgactatc
     1681 ttcaatcaaa aatgctaaag gctcattttt ataaatatct ccatgtaccc agtagatcat
     1741 ggaaaaatgt gcggaaataa agttccataa gccatttcga gcttcttgat caagatagaa
     1801 catttctttt acgtgaaata tttcttcagc tacccagtag aacagaacac ctcttggttc
     1861 atggttgcta tcgtagtaga cagcggctgt gcgctcatct tcattttcga aacgccaata
     1921 ctcttcccaa tgaaaatcac tgcggaataa agcaccatgg ttttgttggg caaatttgtc
     1981 ataaacggaa aatacgtctg gatgatcaac tggtaatcgc tcaaccatac cacttactgg
     2041 gatttgttta ggtagctgtg tatctctgat tttaaaggaa agtttatcag acatgatttc
     2101 ccatccttta cgccgatagt aggggatact gtaaggatac aaataagaga tccattgctt
     2161 attcttgcgc atattatcca atgcaacgat aattagatct tgcatcaatc catggttagc
     2221 atattctgga taagttccaa ctccagtcac accacccatt ttataacgtg caccgtgaat
     2281 attgacttca caaggataga tagcgatttg agagatcagt ttattttcgt ggaaccatcc
     2341 gaacactttc gatacttcca aaatcggttt tttggaacgg ataaaagctc gtttattctc
     2401 aaacccgctt tcctcgatgt ctgcttcagt cacttggaac acataggaaa gcaattcatt
     2461 gaattgatcg atatgttctt gagtgaccgg ttttaattca agattttctc taaaatcttt
     2521 ttgatccatt ttttcatctc catttattag cttgataaca gtatagcaaa attctacaaa
     2581 aaaaacttct atagctgact aattaaaaac tctttaaatt actgcttttc gttggtataa
     2641 tggaaagtgc tgattatatg aaaagaggag acgctatgga tataaagaaa atgaaagaac
     2701 gccaagagca gattcgtaat ttttcgatca tcgctcatat cgaccatggg aaatctacat
     2761 tggccgatcg tattttagaa atgacgcaca ctgtgacgtc tcgcgaaatg caagatcagc
     2821 tgttagactc gatggatttg gaacgtgaac gtggaatcac gattaaatta aatgctgtag
     2881 aactcaatta cacagcgaaa aatggagaaa catatatctt tcacttgatt gacaccccag
     2941 ggcacgtcga cttcacctat gaagtctctc gaagccttgc tgcctgtgag ggtgccgttt
     3001 tagtggtcga tgcagcacaa ggaatcgaag cacaaacatt ggccaatgtt tatttagcgt
     3061 tagacaatga tttggagatc ctgccagtca tcaataaaat cgacctaccc gcagcagatc
     3121 ccgaacgtgt ccgtcaagaa atcgaagacg taatcggaat cgatgctagt gaagctgtat
     3181 tagccagtgc caaatcaggg attgggattg aagatattct cgagcagatc gtggaatatg
     3241 ttcctgcacc aagtggagac ttagatgcac cgctaaaagc tttgatcttt gactctattt
     3301 atgatagtta ccgcggtgtt gttctaaatg tccggatcac ggacggtatg gtcaaacccg
     3361 gagacaaaat caaactgatg agtaatggca aaacatttga tgttacagag gtcggggtct
     3421 tctcgccaaa agcagttgct agagactttt tgatggtcgg tgatgtcggt tatatcacag
     3481 caagtatcaa aactgtccaa gatacacgag taggggatac agtaacgtta gcagacaatc
     3541 ctgccgctga agctttacca ggttaccgaa aaatgaatcc gatggtttat tgtggtcttt
     3601 atccaattga tacttctcgt tacaacgatt tgcgggaagc tttagaaaaa ctgcaattga
     3661 atgatgctgc tttacaattc gaaccagaaa cctctcaagc attaggtttt gggttccgct
     3721 gtggattcct cggacttctt catatggatg tcgtccaaga acgcttggaa cgggaattca
     3781 atctagaatt gatcacgact gcaccatccg ttatttacca tgttaataaa acggatggta
     3841 cgactgctac agtagataac ccggcagatt ttcctgaacc tgtaacgatt caggatgtag
     3901 aagaaccatt cgtcaaagca cagatcatgg tcccaaatga ttttgtcggt gcagtaatgg
     3961 aactttctca acgaaaacgg ggagaattca ttacgatgga ttatttagat gattaccgag
     4021 taaatgtcgt ctataatatt ccgctttctg aaattgtctt tgactttttc gacaaattga
     4081 aatcaagcac gaaaggctat gcctcattgg attatgaaat gtctggttat caaaaaagca
     4141 agttagtgaa gatggacatc ttgctgaatg gagaaaaagt cgatgcatta agctttatcg
     4201 ttcaccgaga ctttgcctat gaacgtggaa aagcaatcgt tgaaaaactg aaaaaactaa
     4261 tcccacgcca acaatttgag gtgccaatcc aagcagctat tggacaaaaa atcgttgccc
     4321 gttcagacat caaagccttg cgtaaaaacg tcttggctaa atgttatggt ggagatgttt
     4381 ctcgtaaacg taaactctta gagaaacaaa aagaagggaa gaagcggatg aaacaaatcg
     4441 gctccgtcga agttccgcaa gaagccttca tggccgttct gaaaatggac gaggacgaac
     4501 cgaagaaata gcaagggttt gtttgaaaac agtgtaaaaa ataaaccatg tttacatgga
     4561 tttgccaccg gtttgccacc ggtggttttt ttcggtggca aaaatcaaga attctttcta
     4621 agattttcca tgatatcgac cgtttcattt ctcatcttat ctgttacatg cgagtaagta
     4681 tccatcgtaa ttgaaattct gctgtggccc aatcgttcag agatttcttt catttttgcg
     4741 ccattttcga gaagaagtgt agcatgagta tgtctgagag aatggaagtt aaaagagagg
     4801 gagagtgcat tcgatatcct cctagtattc catttcacta cacttggcgt aactagctca
     4861 ccgtcttcct tcgtacatac tgcatttgaa tcaatgtaga gctttccata cttcattcga
     4921 ttttctaatt gttgtttctt atgttttttc agaattgcta gcaaggtttg tccaataaaa
     4981 atcgttcgat tggaactgct tgtctttggc gtaccatata cccatgcgcc gtcattcttt
     5041 accatttgtt tctctacagt aattgttcca tttgaaaaat cgacatcatc ccacgtcaga
     5101 ccacaaactt cgccaacgcg cattcccgta taaaatccaa tattcaaagg aatatagaaa
     5161 ggatgtcctt caggagtaat ttctagcata tgatcaaagt cctcaagaga aatgattttt
     5221 agatcttttt tagtcgttgg tcgtgcttca tattttggta tttttacata cagcatagga
     5281 ttttgcttga ttaatcccca aggataaacc gccatattca gcgcattctt aaggacagag
     5341 tgagtaatag tcattgtttt cttcgagtaa ccctttttaa attcagcatt gatgaaattt
     5401 tgtaaaagag caggggagag atccgtaagt tttttctttc ctaaataacc gtttatatga
     5461 tttttgatgg taaatcggta gttttcatag gtattgtatt ttagatttag tttaacgtat
     5521 tcctccatcc aaaaatcaag gtattgtttt actcgagtat ccgtacctaa aaagtattgt
     5581 cctgtttcgt caatatctga taaaactttt cgtaaagcag cttcggcctc tggtcgggtg
     5641 tctccgccaa ctttctccac tttttttctt gagccatcat cattgatatc ttcgccgttt
     5701 gtataattaa gcgagacaaa taaaaaagcc atttatgtta cactc
//
LOCUS       DACEUF010000114         5643 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.120, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000114 DACEUF010000000
VERSION     DACEUF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.120"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            132..1106
                     /locus_tag="IXG20_002448"
     CDS             132..1106
                     /locus_tag="IXG20_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1610614.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1088..1549
                     /locus_tag="IXG20_002449"
     CDS             1088..1549
                     /locus_tag="IXG20_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1610615.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1599..2063
                     /locus_tag="IXG20_002450"
     CDS             1599..2063
                     /locus_tag="IXG20_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1610616.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2077..2826
                     /locus_tag="IXG20_002451"
     CDS             2077..2826
                     /locus_tag="IXG20_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1610617.1"
                     /translation="MLLKAILLCLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2826..3656
                     /locus_tag="IXG20_002452"
     CDS             2826..3656
                     /locus_tag="IXG20_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1610618.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3656..4303
                     /locus_tag="IXG20_002453"
     CDS             3656..4303
                     /locus_tag="IXG20_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1610619.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKINYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4401..5177)
                     /locus_tag="IXG20_002454"
     CDS             complement(4401..5177)
                     /locus_tag="IXG20_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1610620.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5259..5402
                     /locus_tag="IXG20_002455"
                     /pseudo
     CDS             5259..5402
                     /locus_tag="IXG20_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            complement(5519..>5643)
                     /locus_tag="IXG20_002456"
     CDS             complement(5519..>5643)
                     /locus_tag="IXG20_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1610621.1"
                     /translation="EESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
BASE COUNT         1926 a          765 c         1006 g         1946 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat ttttttgaaa aatagtattg caaaataaag
       61 caatttcata gataataggt atcgtaaagt tatataactt tataatacct attagagtga
      121 gggataaaga aatgagacaa ctacacaaag aacagattga aacagcaatg gcagcgttaa
      181 aggaaaagac aattgatcat gtatatttta cagcatgtgg gggttcacaa gccgttttaa
      241 tggctggtca atatctattt gataaagaat taactgtacc ttgtcatgtt tatactgcta
      301 acgaatttaa ctatgctata ccaaaaggat tatctgaaaa ttctttagtt atcacttgtt
      361 cccattctgg cacaacacca gaaactgtag aagcaacaaa aaaagcaaga gaatttggag
      421 caatgactat ttgtttatct aatgaagaag gatctcctct atgggaagca gctgaatatc
      481 ctgttcacta tgattggggt aaagatgttg atgctagtga tttaaataaa ggaattcttt
      541 atggtttatt atttaatatt ttacttgttt tagatggctc taaaaaatgg gaaacttgct
      601 tgaatgaatt tgataagtta aatgatcttg ttattgctga aaagagcaaa tttgaaataa
      661 ctgctaaaga atggggtaaa aaccaaaaac gagataaaat tatttacaca attggtagcg
      721 gtattaatta cggagaaatt tactccacag cagcttgctg gttcatggaa atgcaatgga
      781 ttaattctaa tgctattcat tctggggaat ttttccatgg tccttttgaa atcactgatt
      841 atgatgtacc attcatctta gtgaaaagta ttggagatac aagattttta gatcaacggg
      901 tagaagactt cactaaaaaa ttcagtaata aattaatggt tttagaccaa gctgattttg
      961 acttagaatc tatagatagt gtagcgaaag aatatattgc agctgtactt agtggcgttg
     1021 ttattcgcca aatggtagaa gcgattgctt ttgaaagagg ccattcctta gatgttagac
     1081 gctatatgtg gcaaatgaaa tattaattaa taggaggttg caaaagatgg caaatcaatt
     1141 atttcacatt gtgattacaa cacattctac cctttgtgag ggttatttaa aggcaacaaa
     1201 attaatttta atgaatgacg ccacaggtat tagtgctgtt ccatttaaag aaaatactag
     1261 tccagatgat tttgaggaga gtatgaaaga aataattagt cagcatcatt tgcaacctct
     1321 aattatttta acggatttaa ttggaggaac acctaataat gtttctatta aacagttgaa
     1381 caacaataga attcaaatta ttgctgggat taatttacct cttttactgg agttattaat
     1441 ggcccagtca gctggtaaat ctatagaaga aatagatttt aaggaattac tgaataactc
     1501 tcaaaaaagt atggttcata ttaattcgct tttaaaagat gtcagttaag gtggcaattt
     1561 ctattctata aattttaaaa attgaaggag gaaatagaat gattaaatta gtgcgtgtag
     1621 accacagatt acttcatgga caagtaatat tttcttggac taaacagtat gatattaacc
     1681 atattattgt ggctgataac aaagttccag gagatccaat gtctgtgatg gctttgacaa
     1741 ttgctaaacc tgctgattgc aaattagaca ttgttaaaat tagtgatgtt aaagggatta
     1801 tggaaacatc cccaaatgat aattacatga ttttaattaa aggacctaag gaagcttttg
     1861 aattagttca aaatttacca gaaattaaag aaattaattt tggcggtgtg tctaagaaaa
     1921 gcgagtccaa acaatatgga aaggcagtct ttttaactcc tgaagaatta gaagccacaa
     1981 aaaaaattct tgatcaagaa gtgaaaatat ttgtgcaaca agtcccatct tcaagtattg
     2041 aaaatgtaga ttttagtaag taagggggga aaataaatgt tattaaaagc cattttatta
     2101 tgtttagttg gtgtggtagc tgttattgac tccaggttaa ttggtcgaca aaatattggt
     2161 agaccactaa ttttgagtac acttgccgga cttgtgctag gtgacttgca tacagggatt
     2221 gttcttgggg catctttaga attaatttct atgggattcg tctcaattgg ggcggctgga
     2281 cctcctaata tgcaacttgg tagtattatt gccactgctt ttgcaatttt aactaatagt
     2341 agtacagaaa cagccttaac aatagctatc ccagtggctg tagctggtga atttatgagt
     2401 attatcatgc gcatgataat tgctcaattt ggacatgctg cagataaagc cattgaaaat
     2461 aataaattta gaaaagctca aattattcat atttattggt catttatttt taatgctttt
     2521 gtttacttta ttccaatctt tttaacagta tattttggtg ctgacgttgt ggcagattta
     2581 gtagataaaa ttccagaaat gattacagac gccttgactg tctctggaaa tatgctttcc
     2641 gcattagggt ttgcaatgct attaagcaca atgttgagta aaaaattata tccttacttt
     2701 atttttggat tctttattgt tgcctattca ggtctttcac taattggcgt gacaattttt
     2761 gcagctctga ttgcctttgt aatggatcaa gttaaatatg gaaaaagaga agaggcgcat
     2821 ggttaatgga aaatagtaca gaaaaaaaac gtttaatcac aaaaaaagat cttcataaga
     2881 tgtgtcttcg ttcatggaca atcatgtgtt cgtggaatta tgaaagacaa atgcatatgg
     2941 gatttatgta tgggatggcg ccaattttaa ataaagttta taaaaatgat gaagagaaaa
     3001 aaagagaagc ttatactcgt catatggcat tttttaactg tacgcctcaa atgactccat
     3061 ttattatggg actttgtgct tctatggaag aacaaaatgc tcaagcagaa gaaggaaact
     3121 ttaatactga atctatctcc atgattaaaa ccagtttaat gggtcctttt gctggaattg
     3181 gtgattcatt tttccaaggt actttaagaa ttattacatt tgggattgga ttgtcttttg
     3241 ctaagcaagg tagtatttta ggaccgattt tagctgttgg cctttttgct ataccttcta
     3301 ttttagctgt tttttacggc ggtaaattag gatatgagtc tggcaataaa taccttacta
     3361 aattgtatca agaaggtctt atggatcgag taatgcaatt tgcttctatc attggtttgg
     3421 ctgtagtagg tggaatggtg gctagcatgg tgccaattgt caccccttta aaattcacta
     3481 cagggggaac agaacttgtt ttacaagact tacttgattc cattattcct ggattattac
     3541 cacttgtatg tactttggga atatataaat tgattaagaa aaatgttagt acaaatatgc
     3601 tcttattagg ttgtattttg gcagggtttg ttttaagtat aataggtatc ttataatgat
     3661 taaggctcta atttttgata tggacggagt tttaattgat agcgagatta tgtatcaaaa
     3721 gtgggtattt aattttttaa aacaagaaaa tttaagctat cctttgtatt cttatcaaaa
     3781 aaagattgga actacagcaa gtatttttga tgatatagaa aagtacaatg agggatgtaa
     3841 cagtttagaa ttgaagggaa aatttactaa atattggaaa tcgcaaaaga ttaattactt
     3901 ggatattttt cctaatatac taattgaaca gcttgaatgg ttaaaaaaga aaaaaataaa
     3961 aatggcaatt gcttcttcca gtccaatgga tactataaat gaagttgtta ccacttgtaa
     4021 aatagagtca tattttgatt gtttaattag cggaagagat ttgccagaaa gtaaacctaa
     4081 tcctactata ttccttaaag cttcagaaca gctcagaatt ccagttgaag aatgtctggt
     4141 gatagaagat tcttataatg gtataaaagc tggtaaaaga gcaaatatga aggttcttgc
     4201 aattaaagat aaacgttttt ctcaagatgt aagtctagca gatgatgtta tttacgatat
     4261 taaatatctg cgacaagaag tacaggtgaa atttggaata taaataagtt ttttgatgta
     4321 tttagagaat attagtaaaa ataaaaatca tgcccaaatc taatcaaaat tgattgcttt
     4381 gggtatgatt tcttttaata tcaatctatt actttaggat ctgaatcatt tttatgcacc
     4441 gtatctaaag tgatattttc tgtgacttca ataacataac taattctatc acaacgaact
     4501 gtggattgag aataatgaaa tggttgatta tttttatctt tgccaacctt ttctaaaata
     4561 aatagcggat ctcctactat acattgcaat aagtcggaaa gctcaggtgt tgcagataaa
     4621 ccgtttattt ccataataga gctcgtagct ttgacatcat agtcatcttc tagaagttta
     4681 tataatgaag tatattggct acctttttca aataaagttg gaaatttttc ttctgatgcg
     4741 aaaaaattat caattcccat aggggcaccg tcttcccaca ttaaacgttt aatattgtaa
     4801 actctatcag ttacattgat ttgtaattta ttagctaaaa actcatcagc ctttataatt
     4861 ttagaatgta aaacagtata tctaatttta tgtcctcggg tagaaagaga atcgctaaat
     4921 ccatgaactc cagctaattt taaactttct tttttttgag ttacaaaggt ccctttcccc
     4981 cttaatcgtt caactgttcc actttctgtt aaaagtttaa aagcttttcg aatggtaatc
     5041 ttacttactc cataaatttt acataattct tcttctgtag gtaattgctc tcctggaaga
     5101 tatttttttt cacgaatatc gttacgtata gaagcttcta cttgtagata taatggtcta
     5161 ttatcttgaa aattcatagt acactcccta aaaagttata cttcattata tcacaattta
     5221 ttaaaataaa agattaagat ataaatataa atgaactagt ctttgactaa ccaatgcccg
     5281 cttttgaaag aaaacaaatt cattctgttt taagtaaaaa tccttatgtt accactcatt
     5341 ctgaagggga tgagccttat cgctacttgg ttgtggaacc aagtgataag acaattttat
     5401 aaattcaata ttaattaaaa taaaaacaaa caaagtagat tattttactt tgtttgtttt
     5461 tttattggga gcgtcaataa ttttgtgtaa ataactcgtc cttctgcaaa ataatgggtt
     5521 actcagtaaa cattgaagct aatgtatccg tcacttgttg gaagccttta tggcttctat
     5581 ttaagaattt ttgattgtat gtatcaaaga tggatactag gaaacgctct agtgattctt
     5641 cat
//
LOCUS       DACEUF010000115         5339 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.121, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000115 DACEUF010000000
VERSION     DACEUF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.121"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(19..414)
                     /locus_tag="IXG20_002457"
     CDS             complement(19..414)
                     /locus_tag="IXG20_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610622.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
     gene            complement(404..616)
                     /locus_tag="IXG20_002458"
     CDS             complement(404..616)
                     /locus_tag="IXG20_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1610623.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1688..2290
                     /locus_tag="IXG20_002459"
     CDS             1688..2290
                     /locus_tag="IXG20_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610624.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            2645..2881
                     /locus_tag="IXG20_002460"
     CDS             2645..2881
                     /locus_tag="IXG20_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAQ1610625.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            2878..3291
                     /locus_tag="IXG20_002461"
     CDS             2878..3291
                     /locus_tag="IXG20_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1610626.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            3810..4760
                     /locus_tag="IXG20_002462"
     CDS             3810..4760
                     /locus_tag="IXG20_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HAQ1610627.1"
                     /translation="MERNSENKMTGRNPKVFISYSWENKENKEWARELADKLLENGID
                     AYIDQYDLSLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAEL
                     LSGKNERKFIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKP
                     ALGDIPRHIKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRV
                     PSQLWKELFVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIE
                     KSNTQEKKILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(4757..>5339)
                     /locus_tag="IXG20_002463"
     CDS             complement(4757..>5339)
                     /locus_tag="IXG20_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1610628.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1908 a          760 c          902 g         1769 t
ORIGIN      
        1 ttgtatgaac ctttatttct acattttttt ctcaagcaca ctactgataa tgattaaaat
       61 aatcggctgg attgcaatga tacttccgac aataatagct ggaatgatgt agtctaaaca
      121 aataaatata actgttgcca cccctaacag tattgtgtta aacatatttg ttatatttcc
      181 cgctttttct ttgattatga tattccgttc gtccataaca ttgattttgt agtctgcatc
      241 atttttaatc attttatcat gcaaaatttg aacatgagaa acgccaaaaa cggcactgaa
      301 taaaatagca agtccaatat ctaccatttt aggaaaatct gtaaccaaaa ttgtcacaat
      361 caaaaacaac gaaacaatat aaccgatata ccaaatctga cccttatttt tcatcttcac
      421 tttcctcctc atagataaaa atttcttcaa tgctcatttg aaagtacctt gcaattttaa
      481 atgccaatat aatagaggga ttataacgtc cattttctaa agaacctatt gtttgacgag
      541 atacttcaag agcagaagct aaatcttctt gtttaattcc tctttgtttt cgtaattctt
      601 ccaaacgatt tttcacaaca tgctcctttc taaaaaatgg aaagtttact ttcctttcgt
      661 gcttttataa tactcctgcg agcacaaaat gtcaaggaag ctttccatat aaattttgat
      721 tttaatgttt tagttactgt ttttatgaca catgaacaca cttattctta tttcctctta
      781 tctgctatca acagaaaaga aaaagccctg tcaagagccg attagatcaa gccagtcgag
      841 aaacgatgtt agaccaaatt gattttgggt aatgaatgtt tagaacttag tagtcatcca
      901 aaaacgacta ctttttttgt tgaatcgtcc aaggacgatt cgatgctttt gacttgtaag
      961 aacaggattg aatgaaacca tactgttcgc atatttttag tggatgaaca aacaaaatac
     1021 gagagatttt ttgttcgttc atccatggtt ttagaaaaaa gagggacgat tttggaagaa
     1081 gaaaatcgtc tctttttttt cttctttttg tatgacaaaa agaaagatct tttgcccatt
     1141 ttatttttat aaaatgggta ggtggcgttc gcgtaaagca aatcgataca atccaatggt
     1201 tttggcgaat cagatttacc attctaggct tgtttaatac agacatcgat tgttattttg
     1261 agaatttata tccttattgg caatgaaatg gatattctcc ataccaatag aaatcttcaa
     1321 gataattttc ggaaatatat aagagttaat caattatgat catatagtag gtaaaaatta
     1381 ggacagcctt caattatatg ggaaaccaat ataatcaagt aattattcgg gtaacacaaa
     1441 tactagagta ctcatttctt ataactgttt tacacctatt agggatcagt attattaata
     1501 gatctagata aattaatacg taattatata ataatttatg tttgtgccat taaacaatct
     1561 taacagatat ttcattttat ttatgtaaaa caacacgaat aattattgct tcgtaaagtt
     1621 atatgagtat aagttaattt actggaggta gtgcaatgct tttttctgaa agatttggtt
     1681 ttaaaaaatg gaggatatca tactgagaga tagattgaca gaccctctta aaaatagttt
     1741 agcaaatgtg atttatgata tattacgaga ggctgataaa gtttatagta attataatgg
     1801 aaaagtctca tgggtaatat attctttttt aattaaagag ttttttaaag aacagataaa
     1861 taatagctat gagttgggtt atcattttac tgtttattat gaagatatta tcaagaggtt
     1921 agaagacttg gagtggtatg cttattatga tttattagag gtttttgcaa gtgctaaaat
     1981 cttggataaa aagaaaagag aggaagtaaa taaaattctg gagtcggaaa attctggtta
     2041 cagattaagc gaaagcaatg aatttattcc aatcacagat gatatttcta ttaagtctat
     2101 aaatgctgta tctgaatcac catttaatta cgcaaaaaat catatccata aatctcttac
     2161 atatataagc gagaaggaaa atcaagatta taatactgta gtgagagaag caattagtgc
     2221 tgtcgaaagt tgtcttatcg aattttcagg attaccagct aataaaaaaa tactttggga
     2281 actgctataa aagcaattaa agacaaatcg aataacaatg tcgatcttac atttttaaaa
     2341 ccatttgaga caatgtatgg tttggcgagt aataatggaa tacgacatgc agaaaatgaa
     2401 aaaacaattg tctcagattt acctgatgca gttttggtgt taacaacatg ttcagccgta
     2461 attaattatt tgggagtaaa aatagcatat gactccaata aatcatgaaa agttaaaatt
     2521 catccaagag ctacattata actattaatt tccaactctt taaaagttta gtaattgaag
     2581 gaagtaataa gggtgattga acaatacgga tatacggtgt atacttatcg taggagttga
     2641 tgatatgaat attaaaaaac gcaaaatcag agaagtaggt aattcaatag ttgtaacttt
     2701 gtcaaaagag agtttattgc aaaaaggttt gaaacctgga gatactattt ttattgatga
     2761 agataaaatg atggaagcta ttgtaaaaga ggaaagcaag atagatcttg aaattgatat
     2821 gtatgttaac caagctttat ctgagtatga tgcagctttc aaggaattag ttgaaaaatg
     2881 atctatctaa gtgaaaaaga tattgtaaaa ttgaatgtta ttcaaataaa gaaatattca
     2941 ccgcgagaaa caatcggagt cattgacaaa aatgcgctga gtatggctgt aaatcaacct
     3001 aaacagcata tttttggaaa agatctgtat cctgataaat ttagcaaagc agctgttctg
     3061 ctaataaatc tgatcaaaaa gcatccattt cataatgcta acaaacgtac agcattttta
     3121 gccgcgtatg tttttctcaa gctaaatggc tattcactaa aaatgaaaaa tcaggaagta
     3181 gtagaatttg tagttgaaat tgctacgtat cagggtgatt ttgatgactt aaaagaggca
     3241 acaacagata tactaaaaaa gaattcaaca aagaccgatt caaaaaagta gaagcaaact
     3301 agggattcag ttaaatctga agatttagtt tgttctttat tttagtttac ataagataca
     3361 ttatgcgaag ttcattattt taaaagctta aataataaac ttttttataa taccaattta
     3421 tttatgtaaa ccaatgacct taatcgctta agcaagcatg ttatgtaata ctctactctc
     3481 tccaaaacta atgactatct attttcaaag aagctagaat gattatctga tcaaatgata
     3541 atttatattt aattcctggt ctactgctct tcttttaatc aattaatgtc taacgtactg
     3601 tacacgataa agtaggaaga cttattttca acttcggatt cattttttta attcggtgaa
     3661 aatcactaaa ttaagagttt atccaagttc attttagctg agaatacgct tgaaaataat
     3721 tttgatgaat tttcctgaat taaaatttat taacttgttg agtgatataa ttgtaatgta
     3781 ttaccaaata gttagcactt agattagtga tggaaaggaa tagtgagaac aagatgactg
     3841 gtagaaatcc caaagtattt atatcatatt catgggagaa taaggaaaat aaagaatggg
     3901 ctagagaact agcagacaaa ttacttgaaa acggtattga tgcatatata gatcagtatg
     3961 atttaagttt aggtgataga ttacctcagt ttatggagca gcaaatatcc gattcagatt
     4021 atgttctaat tatatgtaca ccagagtata aaaaaaaggc aaataatcgt acaagtggtg
     4081 ttgggtatga aggtcatatt atctcagcag aattgctttc tggaaaaaat gaacgtaaat
     4141 ttatcccaat aattaaagaa ggacgtgcaa gcgaagtact tcctactttt cttgctggaa
     4201 agcttggaat tgatttatca gatagccagc attatgatga aaacttagat gatttaatta
     4261 caactatatt tggtgttaag agaaaaccag cattaggtga tatacctagg catattaaag
     4321 gcagtccttt actacctata gaacataatg aaaataaaga tattgaaatc ttaggaatta
     4381 tcactgataa agtaactgta cctaaaatgg atggctcaag gggttctgca ttatataaaa
     4441 ttccatttag attaaataga gtaccaagtc agctttggaa agagttgttt gttagaaatt
     4501 ggaattcacc accacgatat acaacaatgc atagaccagg aattgcttct gttgtagacg
     4561 atgaaatttt acttaatgga acaactattg aggaagtccg ggaatatcat cgggaaactt
     4621 taaaactttg cattgaaaaa agtaatactc aagaaaaaaa aatattgcta gaggaggaac
     4681 aaaaaaaaga gagagagaat gagcgagtca gatcacataa agaaagtgtt cagaaattag
     4741 cagatgaatt agagttctag ttgatagtca gttctttctt aattcgatac acagtttgtc
     4801 tagttacatt atagtctgtt gctatttttg aaatagcaac accattctcc tggtcctgaa
     4861 cgatgcttct ataaacaagg cgacgttgag gatcttttgt gttcgcactg tataacttag
     4921 gacgtccttt atagacgccg tttgctttag caatctttat cccttgtgcc tggcgtcgtt
     4981 ttgattcagt acgctcttgt tcagcaatca tggccaagat ttgaatgatt aggtctttga
     5041 taaatttatc caggagagga tttccgattg cttcagccat gatcggtaaa ttagtgataa
     5101 taagtcgcac atttttcttc tttagataat ttactgaatc gataatctcg tcataattac
     5161 gtccaagtcg atcgatagcc tctacaataa aaatatcttg gtctctaaca aaatctagaa
     5221 cttcctgaaa aatcggtcga tgagtaatag tcgctccaga ttgtttctcg atgaaaattt
     5281 tttcagcacc aaattttctc atttcctcga tttgacgttg ttcattttga tcgatcgat
//
LOCUS       DACEUF010000116         5232 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.122, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000116 DACEUF010000000
VERSION     DACEUF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.122"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            124..1323
                     /locus_tag="IXG20_002464"
     CDS             124..1323
                     /locus_tag="IXG20_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1610629.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(1350..2618)
                     /locus_tag="IXG20_002465"
     CDS             complement(1350..2618)
                     /locus_tag="IXG20_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ1610630.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(2781..3427)
                     /locus_tag="IXG20_002466"
                     /pseudo
     CDS             complement(2781..3427)
                     /locus_tag="IXG20_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(3835..4338)
                     /locus_tag="IXG20_002467"
     CDS             complement(3835..4338)
                     /locus_tag="IXG20_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1610631.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(4461..5225)
                     /locus_tag="IXG20_002468"
     CDS             complement(4461..5225)
                     /locus_tag="IXG20_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ1610632.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
BASE COUNT         1488 a         1141 c          891 g         1712 t
ORIGIN      
        1 tttaattgaa caataagaaa aacatctttc catcggaaag atgttttttt gctttttcta
       61 gtttaaataa cagctttttt catctatttt cgttatactg tataagaaaa aggagttgaa
      121 tttatgaatc taaccagaga tctgatcttt ttagttctct atcttattgg ggtattctct
      181 atcgtttatt ggcgaaaaca gaagaaggat ccttccttgt tcttttacgg cagtacgtgg
      241 acatttacgg taatcttaac ttattttgtc gtgttggaaa ttttttattc gaccttttat
      301 tttttgattc cattgttgtt ctttagtatc tttgcttttt cttatttcac agaaaaacga
      361 aggctactga atggtctttt attcaatgtt ttactcatca gtttcggcat ctacttgttt
      421 gtcttgctgt atgaaacgca aaatattttt cttggcggac tgatcgcctt gatcacgatt
      481 ccattgctac ttgtcctttt atttggtatt tatggattga ttgtttttct tttttggaac
      541 ggggtcacag ttttacgtag agaatcgcat tcattagcta atcttttaac gttgatacta
      601 gccatttttc ttacactgtt tcttgttttt gactttttct tgttaaaata tttgccgcaa
      661 tggataaatg ctttgttttt ctgcgttccc ctgatcttga tctatctatt tatcgtattc
      721 tacaactttt tgactgtctc attcttgtat cagttcaacc ggcctcgata caatcaagac
      781 ttcatcgttg tgttaggcgc tggattgatc aatggagaaa cagtttctcc tcttctagcc
      841 aagcgaatca ataaggcaat tgctttttat cgggctcaaa gccgtgctac attaaatccg
      901 cccatcttat tgatgtctgg cgggcaagga gcagacgaaa aagtaccaga ggccattgcg
      961 atgaaacaat atgccatgga acaaggaatc cctgaaagag acattcttgt cgaaaccaat
     1021 tcgacaacca ctttggaaaa catgctctat tcaaaggaaa tcatggatca gcaaatgaaa
     1081 gggccatacc gcgcgatctt tagttccaac aattaccaca tttttagagc cggtctttat
     1141 gcgcggcaag ccaaattgaa agcaaatggt attggtgcaa aaacagcttt ctactacttg
     1201 cctaatgcgt ttttaagaga atacctcgcc atcttgatga tgaacaaaaa aagacatatg
     1261 atcatttgcg gattgctttt cgcaggctcc gcattcttat cgctgattac tttacttttc
     1321 taaaaaaaca aggtgtgaca aaacatatgt cacaccttgt ttttttcttt cattctctta
     1381 actattggca aaaagagtat cccttttttg accgctactt gctgagcaga ataaataccg
     1441 ctgttgcctg aacacataaa gctgatcaag cagatcataa agaagtatac tgccgcttgt
     1501 gaaccgaaca attcgatccc catcacaaag caggcaatcg gtgtatttgt cgcaccagaa
     1561 aatactccga taaagcccaa tcctgcaaga aaaggaacag aaacatggag caaaggcgct
     1621 agtgctgcac caagcgttgc accaatttca aacaatggtg tcacttctcc cccttgatat
     1681 cccgcgccta aagacaaaac tgtgaagaat aacttcccaa taaaatcata gacatgtgcc
     1741 tgtccattaa atgattcttg aagtaacgga agactcaatc cgagataacg ttgtgtttgc
     1801 aaaatcaatg ctgctgccac aactacagct ccacctatcg tatttcgaca aacaggatga
     1861 ggaatccatt gtgcatatcg cgcttttaaa aagacgatcg aacggctgaa tacccaacca
     1921 atcaaaccaa aagcaatccc agcaacaaaa actttgaaga acagcaacat actccattca
     1981 ggaatcacac ccatttggta atgtagatga gaaacaccat agctttctgt catgaaattt
     2041 gcaaacagtg cagcaaagaa gctagggaag atcgcttctg ttcggacttt cccgattgcc
     2101 aacacttcta atccaaataa tgttccagct aaaggtgtgc caaatacaga actaaaaccc
     2161 gcactgattc cactgataat caagatttcc cgttctgatt tatccaatcg gaacaaacgt
     2221 gccgtattat ctgctaacgc tccccccatt tggactgctg ttccttcacg gccaacagat
     2281 cccccaaata aatgggtagt tatggttcca aacagtgtaa gtggaataag ccgcaacggg
     2341 attttttctt ctccaccatt tccttgatcg atcactaagt tgtttccacg actagaaagt
     2401 ccgccgtgat acgcatagag ataagcaaaa agcgcaccac taactggtag tagacaaagg
     2461 agccaaggat gtgatagtcg gatatctgtc acaactgtca ggctatttaa gaaaaaagca
     2521 gacaagctcc ccatgaatat accgagaatc gctgtgatca ccagccactt cagcatatac
     2581 actaaaatgc gtactggact cggtaattgt ttttccataa aaaattcctc ctcaataaaa
     2641 aaacgcctac taaagaaagg ggctgtcccc agactttagt aggcgtcatt agctatcttt
     2701 cgttagcatt taaaggcgaa caccatcacc tattcacttc cattaaaaga tagcatattt
     2761 ttctgtaaaa aacaataaaa ttatcgttct ttctttacac gttcagcaaa ttcatagaaa
     2821 ggagccaatt tcgttaactg ttgattacat tggtcactat catgacaaat ataattcccc
     2881 tttttcgtat acgtgccatc agaacctgct ttcgttgtag aaagaaaaag cgaaacattc
     2941 gatgttttct gacagattgc acagattccc ttatttatcg ttggtgataa agtaccgcta
     3001 atccctttta atttttcatc ttcgtaatag accatgaatt ttttctgcgt acctggatca
     3061 ttccagccga tgtacgtatg ttcttgcaag tcgatttcat tccaagctgg ttgtttgagt
     3121 tttttcactt ttcggaataa tttctcgatt tgtggtttgc taggctgcat aaacggctga
     3181 acgatttgct tcagctcctc caatgctctt tctgcttgcg cttttgtcag ttttacgtct
     3241 aaaactgtcg ttaaaaatgt atcgattctg gcagttcgtg atctaatatg tctcgaatct
     3301 tttcagctgc tagtgcctga actgtttgta ccgtttttgg atcattgaca gatgagtacg
     3361 catttaataa ctgttctact tctcttatta taaaaaaata ttggtatggt tttattgttt
     3421 gttccatgat tttaaagtcc tttcttctaa ccaaagtgca tactttgtag aaaaaagtga
     3481 tcttctgcaa agtatgtgtt acctgaagat cccggactct tttaaataag atacgttttg
     3541 catcttatca tcaccccctt taatgcttat tattctatgt acatgattca ttcgataaat
     3601 aaaagtaaac cgtatcttga acagatagtc aactgcaaac agaaaaaagg actgtgacaa
     3661 aagtcgtgtc aaagtccctt gtttcgaatg aatggtgttc aaaagctggc atctgcggta
     3721 ataagccgaa acttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt
     3781 gaagctgacc acttctgtca caacctcaat atggtctatt attggtttta tctattaaat
     3841 cgaggattct ttcccaaagt aacgttgcca tctcttacgc atacttgctg acataggctg
     3901 agcggcttct ttgactgcgc agtatttgtc tacgaatgtt acgatatatc cttctttgta
     3961 cttaggcggt gcaatcgttg cgccccacat gtgcttcaaa atgatatctc gctcaagatc
     4021 cgacaattcg gtgatctttt ctgcattctt tacagcaatt cttggatgga tatacgcatg
     4081 tgtgccttca tcaaatttcg tcgtacgcca atcatagtag aacagatcat gcaaaagtcc
     4141 agcacgcgct gttgctttag cattgccacc ccattttttt gcgagcagat aactatagta
     4201 agaaacactg atggaatgct ccaaacgagt ggaatgaaca tgctgtgtgt aattcgctaa
     4261 ttttttcact gcttctgttt ctaaaagatc ttctacataa gacatatatt cttcgtcttc
     4321 acgccatttt tctgtcataa ttatcgatct gccacctttt tgaatttcat tttcttctga
     4381 aattgatcta agtataccac cagtgttcgg attattctag tcgccttgga taaaatagcc
     4441 aaagcttaat agttccttaa tcataaagaa aaatgataaa gaacaatccc tgccgcaacc
     4501 ccaacattca acgattccgc atttcctttc atcgggatat atacgttctg atcagtcaaa
     4561 gacaagattt cctgacttac cccctgacct tcgttcccca tgattattgc gaagttatct
     4621 gatttttcta ctttgtctaa tgcgtgtgct tcttcattta attctgtgcc atacactgta
     4681 aaccctttgt ctttcattaa aggaatcact tctgtcaaat ctttttgcaa cacaggcaaa
     4741 tgataattgc ttccctgcat gctgcgcagc acctttgtac tataaatatc tgccgttcct
     4801 ttgcctaata aaacaccagc catatcaaat gcatctgccg tacggatcat cgtccctaca
     4861 tttccaggat cttgtacatt atctaacaac agccatcctc ctgaaaaatc tggcgtgcct
     4921 tcagccgtta acgccatcac tgctaagatt ccttgtggtg taggtagatc cgatacagca
     4981 tccatgacct catccgaaac tagaaaagta gaaatctctt tgtttgcggc cagccagtct
     5041 ccccattctt tcatgcctcg ctgtgtcaca aaaagttctt ttatttctgc atcagacgac
     5101 gcagcttctt caatcagatg gaacccttcc aaaagatatt cttccttttc ttcccgatat
     5161 ttctttttat gtagtttttt taattctttg attcttggat tttttgctga ttggatctct
     5221 ttcacattcc tc
//
LOCUS       DACEUF010000117         4930 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.123, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000117 DACEUF010000000
VERSION     DACEUF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.123"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            83..970
                     /locus_tag="IXG20_002469"
     CDS             83..970
                     /locus_tag="IXG20_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1610633.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IXG20_002470"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IXG20_002470"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ1610634.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IXG20_002471"
     CDS             2272..2718
                     /locus_tag="IXG20_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1610635.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IXG20_002472"
     CDS             2991..4343
                     /locus_tag="IXG20_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ1610636.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IXG20_002473"
     CDS             4514..4801
                     /locus_tag="IXG20_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610637.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1693 a          813 c         1030 g         1394 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt
//
LOCUS       DACEUF010000118         4807 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.124, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000118 DACEUF010000000
VERSION     DACEUF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.124"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IXG20_002474"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IXG20_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ1610638.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="IXG20_002475"
     CDS             complement(3250..3768)
                     /locus_tag="IXG20_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ1610639.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1579 a          786 c          730 g         1712 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccactta
//
LOCUS       DACEUF010000119         4727 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.125, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000119 DACEUF010000000
VERSION     DACEUF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.125"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            131..1276
                     /gene="tgt"
                     /locus_tag="IXG20_002476"
     CDS             131..1276
                     /gene="tgt"
                     /locus_tag="IXG20_002476"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ1610640.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1373..1753
                     /gene="yajC"
                     /locus_tag="IXG20_002477"
     CDS             1373..1753
                     /gene="yajC"
                     /locus_tag="IXG20_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ1610641.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
     gene            2058..4655
                     /gene="adhE"
                     /locus_tag="IXG20_002478"
                     /gene_synonym="adhC"
     CDS             2058..4655
                     /gene="adhE"
                     /locus_tag="IXG20_002478"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ1610642.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     AQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         1655 a          835 c         1010 g         1227 t
ORIGIN      
        1 cataaaaaaa ggataaaaaa aagaatatta aataaaatag aaggaagccg agggaacttg
       61 caaaaagcac ttgaaacgat tatgatggtg ggagttgtca agatatttta tatagaaagt
      121 agggaaatga atgacgcaac ctgccattcg ctatcgttta atcaagaaag aaaaacatac
      181 tggcgcgcgt ttaggggaat tgatcacgcc acacggaact ttccccacac caatgttcat
      241 gccagtagga acattagcta cagtaaaaac aatgtcccca gaagagttga aagaaatggg
      301 tgctggcgtt attttaagta atacatatca tttatggcta cgccctggtg aagacttagt
      361 agaagaagct ggcggactgc ataaatttat gaactgggat caaccgattt taacagattc
      421 aggcggcttt caagtttttt cattaagtga tatgcggaat atcgaagaag aaggcgtgca
      481 tttcaaaaat cacttgaacg gttcgcgtat gtttttatca ccagaaaaag caatcaacat
      541 ccaaaataaa ttgggctctg atatcatgat gagttttgac gaatgtccac cattcgatga
      601 aagttacgac tacgtgaaaa aatcagtaga acgtacgtct cgatgggcag aacgaggatt
      661 gaaagctcat gctaacccag accgtcaagg attatttgga atcatccaag gagcaggatt
      721 tgaagatctt cgtcgtcaaa gcgcgaaaga tctagtcggg atggatttcc ctggttactc
      781 aatcggcggg ttatctgtcg gggaacctaa atcagaaatg aatcgtgtat tagaattcac
      841 tacaccgttg atccccgaaa acaaaccacg ttacttgatg ggtgtcggtg ctgctgattc
      901 attgatcgat ggtgttatcc gtggagtaga catgtttgac tgtgtcttgc ctactcgaat
      961 cgctcgaaat ggcacatgta tgacttctca aggtcgtcta gtagtgaaaa atgcgaagta
     1021 tgctcatgat ttccgtccaa ttgatgagaa atgtgattgt tatacatgta aaaactatac
     1081 acgtgcctat atccgccatt tgatcaaatg tgatgaaaca tttgggattc gtttgacttc
     1141 ttaccacaat ttatacttct tgttgaatct aatgaaacaa gtacgtcaag caatcatgga
     1201 tgataatttg cttgaattta gagaagcctt ttttgaagaa tacgggttca ataaagaaaa
     1261 cgcaaaaagc ttctaaagga aaatctttta aaaattaagt aaaaaactag tgttagcagc
     1321 atttttctgc taaagtgttt tttgtagata cagaattagt gaggtgaaaa aaatgggaac
     1381 attaccaatg cttgtgatgt ttatcgcgct attagcaatg tggtttttta tgtcacgttc
     1441 gcaaaagaaa caacagcaag aacgtcaaaa cttgttagat agcatgaaac caggtgattc
     1501 agtcgtaacg atcggcggat tacatggagt tgtttcagaa atcgacaatg acaaacggac
     1561 agttgtttta gactgcgaag ggatcttcct tgagtacgat cgtgcagcaa tcaaaacggt
     1621 caaaccaggg actgtcgtaa caaatgatgc aacaactact gttgaaacaa cagaagaacc
     1681 agctgagaca gttgaagtaa aaactgaaac aacagaaacg ctggaagaga cagaagagaa
     1741 gaaagaaaaa taagtatcaa agcttattta aagacgaggt agggcagaaa gatgtcctac
     1801 ctcatttttt attcaaaaaa gaaagagata agagtatagg atatattgct caatattgaa
     1861 taatcgggtg tgtcatataa ataataataa ataactttat atatttttct atacaaacgc
     1921 ctataaataa agcaacttta tatttaaaaa taactgtgaa aaaaatcaca aaaaagtgtt
     1981 tacatagaaa aataaatatt gtatgatata catgtgaatg atgtaacaaa taaccatgac
     2041 ccattaggag gataatgatg gctaaagtaa tggaaaaaga aaaaacgaag acaattgacg
     2101 tacaagcaat gattgatgat ttggcagaaa aagcaaatgc agcattgaaa gaaatggaaa
     2161 gctttgatca agaaaaagtg gaccatatcg tgcatgaaat ggcaatggca gctttagatc
     2221 aacatatgcc attagcgaag atggctgtag aggaaacagg ccgcggtgtt tacgaagata
     2281 aagcaatcaa aaatatgtat gcgtccgaat atatttggca taatatcaaa catgataaaa
     2341 cagttggtgt aatcaatgaa gatgcccaaa aaggattgat tgaaattgct gaaccagtcg
     2401 gtgtggtttg cggagtaact ccaacaacga atccgacttc cacaacgatt ttcaaatcaa
     2461 tgatcgcgtt gaaaacaaga aatccaatcg tttttgcttt tcatccaagt gcccaaaaat
     2521 cttctgcaga agcagcacga gtagtacgtg atgctgctat cgctgccgga gctcctgaaa
     2581 attgtatcca atggatcgaa catccttcga tcgaagcgac ttctatgcta atgaaccatc
     2641 caggtatcgc gatcgtctta gcgacaggtg gagctggaat ggtcaagtct gcttactcta
     2701 caggaaaacc agctttaggt gtaggtccag gtaacgtacc agcttatatt gaaaaaacag
     2761 caaaaattaa acgtgcagtg aacgatttga tcgtttcgaa aacatttgat aatggaatga
     2821 tttgtgcttc tgaacaagct gtgatcgtgg ataaagaaat ctatgcagca gtaaaagcag
     2881 aattccaagc acaccaagtt tatatcgtaa aaccagatga attgcaaaaa ttagaagatg
     2941 cagtgatgaa cgaaggaaaa tatgcggtca atccatcaat cgttggacat tctgcgatgg
     3001 aaatcgcaaa attggcagga atcagtgtac caaaaggaac gaaaatgctg attgctgaat
     3061 tggaaggcgt aggcccagat tatccgctat caagagaaaa actttctcca gttcttgcca
     3121 tgatcaaagc taacaacacg gaccatgcgt ttgatttgtg tgaaggtatg ttgaatcttg
     3181 gaggattagg acatacagca gttatccatt cagaaaacga agagttgcat gtcaaattcg
     3241 gtcttcgaat gaaagcttgt cgtattttag tgaatacacc atctgcagaa ggcggcatcg
     3301 gtgatatcta taacgaaatg attccgtcat tgactttggg atgcggttct tatggtaaga
     3361 actctgtttc tcgtaacgtc tctgctgtaa acttaatcaa tgtcaaaact gtagcgaaac
     3421 ggagaaataa catgcaatgg ttcaaattac cgccaaaaat cttctttgaa aagaactctc
     3481 tattgtactt agagaaaatg gaaaacgttg aacgtgtcat gatcgtttgt gaccctggta
     3541 tggtccaatt cggttactgc gatacagttc gtgaagtatt ggcacgacgt aaaaatgatg
     3601 tgaagatcga agtcttctca gatgttgaac cgaacccatc gacaaatacc gtatacgcag
     3661 gaacgaaact gatggcagac ttcaagccag atacagtaat cgctcttgga ggtggttctg
     3721 caatggacgc agctaaggga atgtggatgt tctatgaaca cccagacaca tctttctttg
     3781 gtgcaaaaca aaaattcttg gatatccgta aacggacata taagatcgac aaaccagaaa
     3841 aaacacaatt cgtatgtatc ccgacaacat cagggactgg ttctgaagta acgccatttg
     3901 cggtcatcac tgacagtgaa acacatgtga aatatccgtt agcagattat gcattgacgc
     3961 cagatgtcgc aatcgtcgat cctcaattcg tcatgtcagt tccagcatct gtcacagcag
     4021 ataccgggat ggatgtgtta acacatgcaa tcgaatccta tgtttcagtc atggcttcag
     4081 attatacacg tggattgagc ctacaagcaa tcaagttagt ctttgactat ttagaaaaat
     4141 cagtaaaaac acctgatatg gaatcacgtg aaaaaatgca caacgcttct acaatggctg
     4201 gtatggcgtt tgctaacgca ttcctaggta tttgtcactc agtcgcacat aaaatcggcg
     4261 gggaatacgg gatccctcat ggacggacaa atgcgattct tttaccgcat attatccgct
     4321 ataatgcaaa agatccacaa aaacatgcga tgttccctaa atatgactat ttccgtgcag
     4381 atacagatta cgcagatatt gcaaaattct taggtctgaa aggcgaaaca acagaggaat
     4441 tagtcgaagc tttagcaacg gctgtttatg aattaggaaa atctgtcggc atcaacatga
     4501 gtctaaaagc tcaaggagtc acacaagaaa cactagatac aaccgtagac cgtatggctg
     4561 aattagctta cgaagaccaa tgtacgacag ctaatccgaa agaaccattg atctctgaat
     4621 tgaaacagat catcattgat gcttacaacg gataagcaaa agaaagcgta gaaaaaagat
     4681 aagaaaatag atgaaaaaga acttggaaaa cgttgtttct ccaagtt
//
LOCUS       DACEUF010000120         4655 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.126, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000120 DACEUF010000000
VERSION     DACEUF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.126"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(<1..217)
                     /locus_tag="IXG20_002479"
     CDS             complement(<1..217)
                     /locus_tag="IXG20_002479"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1610643.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKP"
     gene            complement(328..582)
                     /locus_tag="IXG20_002480"
     CDS             complement(328..582)
                     /locus_tag="IXG20_002480"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1610644.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(775..1050)
                     /locus_tag="IXG20_002481"
     CDS             complement(775..1050)
                     /locus_tag="IXG20_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ1610645.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1022..1975)
                     /locus_tag="IXG20_002482"
     CDS             complement(1022..1975)
                     /locus_tag="IXG20_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1610646.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2587..4080
                     /locus_tag="IXG20_002483"
     CDS             2587..4080
                     /locus_tag="IXG20_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ1610647.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4215..4511
                     /locus_tag="IXG20_002484"
     CDS             4215..4511
                     /locus_tag="IXG20_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1610648.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4535..>4655)
                     /locus_tag="IXG20_002485"
     CDS             complement(4535..>4655)
                     /locus_tag="IXG20_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1610649.1"
                     /translation="FIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1525 a          772 c          810 g         1548 t
ORIGIN      
        1 tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt aaatttaatt
       61 gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt aataaaactt
      121 gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct
      181 ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta taaaaataat
      241 agacacttct agggatttct aataaccgac acaacaaaga aacagtgtaa tccttacacc
      301 atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg ctgcggcttt
      361 ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt cagctacttc
      421 ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt tgtaaatcgt
      481 tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag tttgattcag
      541 ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc atcctctcgt
      601 agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga tactaacacc
      661 aaatgttgta tacccccaaa caatacacat attttgaagg attaggatta ataaagctaa
      721 aaatatagta agtatactaa atatatttac tatatctagt atacttacta tattttaatc
      781 ttttgatcgt ctattatata taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc
      841 cattgcaagt ttgactattt catacatagg aatatctttt gcataactaa gatcccgaat
      901 ggtttttagt acgggaggat ctacttgaac tggttttctt tctttcgcag tgtactttcg
      961 ttcccctaac tcgacattaa tatcatcttt aattgaaata tcttcagcac caattttctt
     1021 cttatctttg ttcgttggta ataatgccat ttagtttaat ctcctttcca agaggtagga
     1081 tatgattggc cattgagtca gcatatttag gtgtataatc aaatccttca atatcaccat
     1141 gttgttcata gtattcaatt ctttcttcta attctgtaaa gatgtctgca aaaatagccc
     1201 accatcttcg atctacataa tcatttaatt ggataccatt ctcaccatac atttctaacc
     1261 gatccgagcc tttaacaatg cttttaaaaa tgtttgattc accatactta tcaattgttt
     1321 tttgatagtt tgcgtgttga gaggaacgtc gcactgagaa aagaacaggc aaaattccaa
     1381 tgatttgaag atgagactct tcaaaattcg tgacaatcgg aactaaaact ttattaataa
     1441 aatcttctgt tccttccatt gcgtaccgtt ttaattcttg cattgcaata atataatctg
     1501 cagcagttaa gaatgcacga accacattat ctgttgatgg tgggcagtcg ataaagatat
     1561 aatcgaaatt ttttctcaat tcttctaaag gtttaatgaa ggccaatgat ctcttacgac
     1621 gatcaggata tttttcggac aagtattcat taagttcttc agatccagga gagccagcca
     1681 taatatataa atgtggactt acttgagtaa tacctgaaac ccagtcccct tttttgattg
     1741 ctgcagacat ggatcgatca aagctagtaa tttgatacgt tgatccaatg ttggaagtag
     1801 tatttctttg ttggtcataa tcaattagca acacgtcttt ttcaaaaagt tgactagaaa
     1861 caatcgctaa taaatcattg tctgtagtct taccaatgcc gccttttcta cctgtaagca
     1921 ttacaacttt tcctagttta tctgatgttt taaatgcagt ccgtaagttt tccatagttc
     1981 cctccaattt cctatactaa atatatttag tatacttcat atacttaata tattcgatat
     2041 atctaatata ctccaaattg ataagaattc agactttata aggaatatag tggatatact
     2101 aaatatattt gctatattca gtatatcaga tatattcaaa atgtcaaaat atatttgtgc
     2161 gttttgcgtt gacaatttag agtgaaaaga ggtaaactta attctataga aaccacagat
     2221 aattcactat ttacaaagta gcaaaagcag tggtactctt taaacagaaa aattacataa
     2281 aaaaaagaga cggaaataat tgattttagc agaatcaatt atttctgaat cacatggtta
     2341 ggacatgtgt tcacgctcgt ctcaagaata tgtaagtagc taccaactac ttatattaaa
     2401 agttgatcgg ataccttttc cgattgcctt tattctacca aaaaaaggcg agcaactcaa
     2461 cagaagaacg tttatttagt gtacttaaat taaatattct taaacactga agagagaaca
     2521 catactatgg ctgagattaa agctaaactg tgttctcttt ttgtttgaga aaggacgtta
     2581 caatcgatgt tgagcgaaat ttattcagct attcttaaaa acggcttacg tacatacaaa
     2641 tatcgaaaca gcaccatgaa acctgcaggt catattgatt ccagtaggag tggtgctatc
     2701 tttgcgtacc gtagcaagca attaatgaat gttgggcgtg gtatggtcat tacttcggaa
     2761 gaagctattt tagagaacga aaagaaacta acgcattgga cacctaatac ctatcgcttt
     2821 ggtacttatg cggatgatta tcgaacagtg gttaaaggtc attctgagaa aaacttaagt
     2881 caaatcaaca catttgtagt tgatatcgat tcaaaagaga accatcaagg cgaaattatt
     2941 ttagcctgtt tagatcaagt gggctacatg ccaacgctaa ttttagaatc agatcatggt
     3001 tatcaagttt attttgtact aaagacacca ggctatgtca caaagaaaag caatttcaag
     3061 gtgatcgatg tagcaaaaaa aatttcaaaa acaattcgtc tacagttggc tgaaaaattg
     3121 tccggagtgg atctaggatg taaccacttc ggaattgctc gattcccaca agaaaataat
     3181 attgttttta aagaaatgaa ctatcagtat tcttttgctg actggattca atggtcgatg
     3241 aagcttgaat cagatcaaaa aacaaaagaa gatcgagaac gaaaactaat tgtctttcct
     3301 gagaaaaaag aaattcgtca agtagatgaa ccgtggttcg atatgttact tcataaagca
     3361 aacatcgtcg gcggagaagg acgtcttggt cgaaataatg tgattttcac gctttcttta
     3421 gcttattact cttcaggtta tgcattggat acttgcgaat acaatatgtt cacattcaac
     3481 gaacggttac aggaaccgct atctgaagca gaattagtaa agattgtacg tagtgcttat
     3541 tctggtaatt atcaagcagc aagcagagaa ttcattattg aattgtgcca gcaatgggtt
     3601 tcaccaacct taaaaaataa tgatctgttc attcaacgac gtggctggtg gaaatttaaa
     3661 aagccacgag atcaaagaaa atatagtcat aaaaatgaat ggaaagaaga tctattggct
     3721 tatctaagcg aaaaaagtct tgctagtgag ccttatttga acctgtcaaa aaaagaattg
     3781 gcttcacaac taacgatgcc caagcgaaca cttgataaga tcttaaatga gttacgagag
     3841 gagaataaag tcttctacaa ggtaacacga ggaagaaatg gcggaatgat cattgcttca
     3901 gtcaaaacgt tgttcgcgag ggttattcaa ctaagtaaag aaaagagact agattattca
     3961 acaaggatca cagaggtatt tggactcaac gagagcttag tagaagagac atttagacaa
     4021 ttatcaaccc ccttgaaccc tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga
     4081 aaaataaaag gtgcaaattg ccttatacaa tatatctatg atacttctgt aggtgttgct
     4141 ccctttgcct agctacgtgt aggtggtata cttataactg cgtatgcagt cttatgtatc
     4201 atggaggaag ttgtatgtcg ttaaaaaatt atgtctattc acatcctgtt aatgtgttta
     4261 tcattggtcg tttgggatta tctgtggaag tgttctgtga gctttatggt tttaagcagg
     4321 ggactttgtc ttcttgggtt accagggaaa aaacagttgc ttctttgccg attgagtttc
     4381 ttcatgggtt gtccttagct tctggggaga ctatggatca ggtatatgat tgtttgtgtg
     4441 tgttagagca ggagtatatt gagtataaaa tagctaacga gttaagaaaa aggaagaagt
     4501 atatccagta gtagttttta tttactctta attgtcaaat taagttagac aaatcatcaa
     4561 tacttacgta actcaaaaat acttccaaag gtgttcgata attcagtgat tttctaggaa
     4621 tattatttcg tttcgatgcg acagattgaa taaaa
//
LOCUS       DACEUF010000121         4600 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.127, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000121 DACEUF010000000
VERSION     DACEUF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.127"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            93..422
                     /locus_tag="IXG20_002486"
     CDS             93..422
                     /locus_tag="IXG20_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1610650.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            412..699
                     /locus_tag="IXG20_002487"
     CDS             412..699
                     /locus_tag="IXG20_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1610651.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYDQLKKLI"
     gene            985..1842
                     /locus_tag="IXG20_002488"
     CDS             985..1842
                     /locus_tag="IXG20_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1610652.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1843..2391
                     /locus_tag="IXG20_002489"
     CDS             1843..2391
                     /locus_tag="IXG20_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610653.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            2651..3379
                     /locus_tag="IXG20_002490"
     CDS             2651..3379
                     /locus_tag="IXG20_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1610654.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWSKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLRYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            3409..4536
                     /locus_tag="IXG20_002491"
     CDS             3409..4536
                     /locus_tag="IXG20_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HAQ1610655.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEEVIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYSKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAIDTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIEHVKKIMSFSKKNLDI"
     gene            4500..4600
                     /locus_tag="IXG20_002492"
                     /pseudo
     CDS             4500..4600
                     /locus_tag="IXG20_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1663 a          619 c          946 g         1372 t
ORIGIN      
        1 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
       61 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
      121 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
      181 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
      241 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
      301 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
      361 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
      421 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa agctaaaaga gaagccattg
      481 aaagaaaaat ttttgaattt aatttatgat gaaattgcga ttgatccata taagggtgaa
      541 caaaaaactg gggatttatc aggtatttgg tctagtgggt tcaaatatgc gggcacaaca
      601 tatagagtag cttacgagat tatagaaaac aaggttattc cagtattgtt atgtggaacg
      661 catgaaaact tttatgatca gttgaaaaaa ttaatctagt gatttgttgt cgttcttaag
      721 aaggacggct tcttttttat cgaaaatctg attcgtctac atgactaaag tcgcgagtat
      781 tctctgcttc tcgataaatt aaataacgtc attactttga ggaaagctat aaatcaatat
      841 atttcagctt ttccttattt atgtactacc ttttccttat taattataag gtagtaccta
      901 tatatctttt ttttggtagt aactaaaaat aaatatgtta taatagtatc aaatgaatat
      961 tgctaaaaga aaagaggtag gaacgtggta gcgaagaaat atgtaatcgg taattttaaa
     1021 ggtggcgtag gtaaaagtac gtgcgcgcaa atgttagggt ttgaatcagc agttaatgaa
     1081 ggaaaaagaa ccttgataat tgacttggat atgcaaggaa atacttctga tgtgatgaac
     1141 ttaacgcata tgaacttcac taacgaagaa ggaggcggag aaggtgaacc gattgtttac
     1201 gaaaatactg tgactgacgt gttgataaac aatcttccac ctaaagaggc tgtctataaa
     1261 gtaatcaata acctctatat acttccagca gacatgtcct ttgaaatgta tgatgattgg
     1321 atcaaagacc aatataatga ttcattatcc caattcaaat atatgaaaga aaagctagaa
     1381 ccattatttg atgagtttga cgtcatctat ttagatgtcc caccttctat ttctgtatat
     1441 agtaaatccg cgatgtacat tgcggactgg gcaatcgtcg tcttacagac ccaagtaaaa
     1501 tcgatgagaa atgcgatgca atatcttgag tatatggact tttttgtgaa agaatttgat
     1561 acaaatttat atgttgctgg tgttattcct ttcatgttgg aaagcggtga cgcagtagat
     1621 caggaaatgt acaaacaagc ccaagagatt tatggggaac atttgattaa aaatgttgta
     1681 ctcaaaaatg cacgcttaaa acgatacgac ggatcaggta ttacgatgga gaagacaaag
     1741 aaaggtctct taaaacaatg ggatcgcaaa agccacgaat tatttatcag tattttgaaa
     1801 gaattggaag aacacgagaa ttggtatgcg gaggtggagt aattggctga catcaaagga
     1861 cttgatattt ttgggaacaa caaaggactc gccaaaataa aaaaagaagc agaagcaaag
     1921 aaaaagcagc aagcaattac accatctgaa aataagcaat tagaagtgca ccaagttcaa
     1981 gaaataaaaa cttcctccaa tgttgaaaga cagagtagag gaagaacaaa aaaagttggc
     2041 gcaccaatca gaaagtttga taaggtttat tccaccaagc atcctttaaa actgtcgact
     2101 ttgttaaatg cgacttccag agttttagta gaaaagtatg agactaatat gacaagagat
     2161 gaattgctta gaaaagcttt agatgagtat attaaacaga atcttacgca agaagataag
     2221 caagatttgt ttaatgacgt ggtgaaagaa ctagacttgt tcagagaaaa atatccaatt
     2281 gttccagaat tagatgatcg tggagaaata ataagaacta ccagtcagat aaaacaagat
     2341 accgaagact cactgcgcaa agggtgggga atgaataata aagttagata ataatcaaag
     2401 aagaactaag taactacctt taaacggtag ttacttagtt cttctttgat ttgcgtgaaa
     2461 tagaggggat ttatttttct ggttacgtga ttaaaaaatc caaaaaatga gcataggaac
     2521 ggttatgatt ttagggtgtg ttttaatgag atattgtgat ttcattaaca agtgtataat
     2581 aatctgttgt tgagttagta aataagtaat agagctaact ctaaaataaa actattagga
     2641 ggagatgtag gtgaaaaagt ttaaaaagaa aatatattta ggattggttg ttggggtgag
     2701 tatgtttggg ttcatggctc atactaaaca agtaactgca gatactaacc aaaaagaaag
     2761 gattatgagc caattagatg actctcaaat agagtatgta cccaatgagc taattgtcag
     2821 tttaaaggct acacaaagaa tttcaacaga gaaaaataaa ccgcaaaatt tattgggtgt
     2881 aaaagttatg gtggaaaaag atttgactct taggacaaat gaaagcgatt ataataagcg
     2941 agatcaagta ttattagtga aattgaacga agaaattagt gtggaagagg cagtaagaac
     3001 atttaatgct tctgatatgg tcaattatgc agagccaaac tatgtggttg aactagagcc
     3061 gttgaatgat gttgaggagc caaatacaac tgattcttta ggaaatcgat caattattct
     3121 taacgatcct aattggagta aacaagctaa tttaagagag attagcatgc catccgcttg
     3181 ggaagaaacc aagggttcta aggatgtgaa ggtagcggtc attgattcag gaattgatta
     3241 tcaacatgaa gatctttctg gaaatgtaga tgaaagtttg agatacgatt tttttgacga
     3301 tgatacagat cctatggata ctatgggaca tggacacata ttgccgggat tattggtgct
     3361 caaacaaaca acgaattagg tgttgctgga ataaattggg atgttacgat ggtacctata
     3421 cgtgtatttg gtagtagtgg aggtaccagt ttggcaatct taactgaagc tattaattat
     3481 gcatcccaaa aagggtttcc aattatcaat tttagtatag gtagtgttgt ttatagcagt
     3541 acagaagaag taattaaaaa ctatcctgga ttattcgtag cagcagctgg aaataatggg
     3601 atagatatga ctgataaacc tttttaccca gcttctttgg atatacctaa tattattact
     3661 gtaggaaatt ctagtggtta tgaacgaagt tctgattcaa attatagtaa aacaggtgtt
     3721 gatttattcg ctagagggtt tcgtgttttt agtacatttc taggaagtaa atacacgtat
     3781 atgtcaggta cctcgatggc aacacctcat gtcactggta gtgctgcatt agcgttgtca
     3841 ctagatcctg atctatctac tgatgaatta aaaaaaatca ttttaaatag tgttgattca
     3901 ttttatgaac tatctgattt atgtcttaca gggggaagac taaatacgaa taatgtcgta
     3961 cgacaagtaa cgggtaaact tactgcatct aatatcacat taagattaaa tagtgaatgg
     4021 tcagatgaaa ttgctaaaga gatgtgtcac gttcactgga ttgaccagtc aggaaatgat
     4081 ttgacggatc aagtggttgt tctaaaaaac gaagtggata catctaactt tggcgattac
     4141 gaagtagtat ttgcatcacc tgatttaaca gatcaaatct cagttacagt gaccgtacct
     4201 cgattacgta aacttacgct aggaaaagaa acagcaacca ttgatattgg ttccgcatgg
     4261 gatagtgtca tagccattga tacttttcaa gtgaaagcta tagatgattt tgggaatgat
     4321 gttacaaatc aagtacaggt tactattaat gctgatatag atacaattaa ttcaattcaa
     4381 gcagtgatta agtttgaagt tccagaatta ggcttagccc aaattggtag actgaatatt
     4441 actgcaaaaa atagtacaat caatggattg aatgaagtta gcatcgagca cgtaaaaaag
     4501 attatgtcgt tttcgaagaa gaatttggac atttagaggg tgatacgatt gtcggcatcc
     4561 accataaaag tgtcgtcatc acactcgttg agcgactttc
//
LOCUS       DACEUF010000122         4515 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.128, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000122 DACEUF010000000
VERSION     DACEUF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.128"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..801
                     /gene="prmA"
                     /locus_tag="IXG20_002493"
     CDS             <1..801
                     /gene="prmA"
                     /locus_tag="IXG20_002493"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1610656.1"
                     /translation="LDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGL
                     AIGKNKVTVSEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPG
                     MAFGTGTHPTTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEV
                     AVRSAKENMDMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQ
                     TGTLIVSGIIEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            802..1566
                     /locus_tag="IXG20_002494"
     CDS             802..1566
                     /locus_tag="IXG20_002494"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ1610657.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            1799..4012
                     /locus_tag="IXG20_002495"
     CDS             1799..4012
                     /locus_tag="IXG20_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ1610658.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            4023..4469
                     /locus_tag="IXG20_002496"
     CDS             4023..4469
                     /locus_tag="IXG20_002496"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ1610659.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
BASE COUNT         1585 a          793 c         1003 g         1134 t
ORIGIN      
        1 ttagataaaa atgattttac atcgctgaaa gaaggcgcac tagtcatggc ttattttccg
       61 gaaacgactt tcttaccgga aatcttgcct tttatcaaag atcgtatcac acaattacct
      121 gagtttggtc tagctattgg aaaaaataaa gtaacagtta gtgaagtaga ggaaagcaac
      181 tgggcaacag cctggaagaa atactatcat ccggtgcgta ttacgcgaat gctgacggtt
      241 gcgccaagct gggaaaacta ccagacagaa gatcctctag aaaaaattat cacactagat
      301 cctggtatgg catttggcac aggcactcat cccacgactt ccttgacatt gacagcacta
      361 gaaacagtct tgcgtggtgg tgaaacattg ctagatgtgg gaactgggtc gggcgtgcta
      421 agtattgcag caaaatatct tggtgcaaaa gatgtctatg cctttgattt agacgaagta
      481 gccgttcgtt cggcgaaaga aaatatggat atgaatgaaa tagccaaaga tgtccatgta
      541 tcagctaacg atcttttgac aggaatcgag attgaaagtg atgtaatcgt tgcgaacatt
      601 ttagcagata tcatcttatt gatgatccca gatgcttggc gtttactgaa gcaaacaggc
      661 acgctgatcg tttcaggaat cattgaagag aagaagcaaa tggttctgga tgccatgtta
      721 gaacaagggt ttatcgtgga tcaaattttc cagcaaaaag actggtatgc aattattttg
      781 aaaaaaccag aggtggaata gatgcagcgg tattttttaa aagaatcata tgaatcaaaa
      841 gactattttg ttttagaagg ggaaaactat caccacattg tacgggttat gaggatggaa
      901 cccgaggatg aagttttttt ggcattcaat gatcaaatag ccattgttgc agaaatcacg
      961 gagattactg aagaggctgt ttatctgaaa gaaattaaaa aagaagaaaa tcaaaaggaa
     1021 ctgccaagtt cgattaccat agcgagtggc tatcctaaag gggaaaagct ggattggatc
     1081 gttcagaaat ccactgaatt aggagcgcaa gcttttcttg gtttcccggc atcagcctct
     1141 gtcgttaaat gggatgcaaa aaaacgtatg aaacgacaac aacggcttga aaagattgct
     1201 caagaggcag cggaacaatc ccatcggcag atcgtgccat cagtagagtt attggaaacg
     1261 aaacaagatt tatttgagcg gctagatgat tatgacgtgg tcttggttgc ttatgaggaa
     1321 tcagcaaagc aaggcgaaaa atcaaatgca gccaaaattc ttagtgaaac aaaaccagat
     1381 agtcgaatct tagctgtctt tggaccagaa ggtggattat cccgcgatga agtcgattat
     1441 tttgtagaac acaaagggat actttgtgga ttaggaccaa gaatccttcg tacagaaaca
     1501 gcacctcttt atttattaag tgctgctagc tatcaatggg aattaatgaa tccttctttc
     1561 agctaacgtt cttcattaat aaaagagtat cgacagaaag aattgaaaaa atagtcaaca
     1621 atttgtataa tagaaacaat tatttataca atggatacga aaaatcgttt gaaaaagctt
     1681 ttaaaaagcg aacatttccg taaaaaaacg tataactgca cggattttgg gacaattatc
     1741 aaacagatat ttgtcccgat tcccgtttta ccgaaaaaag aaagggggat cgattcctgt
     1801 gacaaaagaa gaaattttaa cgggacccgg tgtcataaag ctcgttagtc aatacatggg
     1861 tcccgaacat gtagcatttg ttgagaaagc atgcgaatac gcgactgctg cacatgacgg
     1921 acagtttaga aaatcaggcg aaccctacat tattcatcct atccaagtcg caggtatatt
     1981 agcagattta aaaatggatc cccatacagt ggctacaggc ttcttacatg atgttgttga
     2041 agatacagaa atcactttag aagatctgag agaagaattt ggcgatgacg ttgctatgtt
     2101 agtggacggc gtaaccaaat tagggaaaat aaaatataaa tcccacgaag agcagctagc
     2161 agaaaaccac cgaaagatgc tgctagcaat ggctcaagat ttacgagtta tcatggtcaa
     2221 attagctgat agactacata acatgcgtac gttgaagcac ctgcgagaag ataagcaaag
     2281 aaggatcgct caggaaactt tagaaatcta tgcgcctctt gcacatcgtc tagggatcag
     2341 ccggatcaaa tgggaattag aagatacagc acttcgttat ctaaatccaa aacagtatta
     2401 ccgtatcgtc cacttgatgc agacgaagag agaagaacgt gaaaaatacg taagtggtac
     2461 tgttgaagat attcgaatag cgacagaaga gctggggatt tttgcagaaa tctatggacg
     2521 gccaaaacac atttattcaa tctatcgtaa aatgaaagat cagaaaaagc agttcaacga
     2581 aatttatgac ttgctagcta ttcgagtgat cgtagattca atcaaggatt gttatgctgt
     2641 attaggagca atccacacaa aatggaaacc aatgcccggc agatttaagg attatattgc
     2701 catgccaaaa gctaatatgt accaatcttt gcatactacc gtgatcggtc cggcaggaaa
     2761 tccggtagaa attcaaatcc gaacacagga aatgcatgaa atcgctgaat tcggggttgc
     2821 tgcacactgg gcctataaag aaggaaaaaa tgaaaaagta gaaccagatg gtatgacgaa
     2881 acaattaagc tggttccatg agatactcga acttcaagac gaaagctatg atgcttctga
     2941 atttatggaa ggcgtaaaag gagatatctt tagtgataaa gtctacgtct tcacaccaaa
     3001 aggagacgtt actgagttac caaaaggatc cggaccattg gactttgcat acagtatcca
     3061 tacagatatc ggtaacaaaa ccactggtgc aaaagtaaat ggcaaaatgg tgcagcttga
     3121 ttacaaattg aaaaacggag atatcattga gatcatgact tctccaaatt catttggccc
     3181 aagtcgcgac tggttgaaat tagttgctac tagcaaagca agaaataaga tcaaacgttt
     3241 cttcaaagcc caagatcgag aagaaaatgt gatcaaaggc cacgaatccg tggtcaaatg
     3301 tattacagat ctaggattta cgcctaaaga tattttgacg aagaacaaac tgcaagaagc
     3361 actcgatcgt tttaattatc aaacagaaga tgatctctat gcagctgtag ggtatggaga
     3421 agttagcccc ttgacgatgg ccaatcgtct gactgaaaaa gaacgtaaag aacaaaaaat
     3481 cgagcagcaa aagcaagaag cagaagaaat catgaatcag ccgaaaaaag aacctgacaa
     3541 aatgaaagta cgtcatgaag gtggcgttgt cattcaaggg gtagaaaact tactaattcg
     3601 tatcagtcgc tgctgtaatc cgattcctgg tgatgatatc gttggttata tcactaaagg
     3661 cagagggata tccattcatc gtcgagattg tccgaatgtt cagcctgaca aaccaaatgt
     3721 agcagaacgt ttgattgaag tcgaatggga agatacatcg aatacacgaa aagagtatga
     3781 tgcagatttg gaaatttacg gctataatcg ttcaggctta ttgaatgatg tacttcaaac
     3841 agtcaatgcg ctaacgaaaa atctcaacag cgttgaagca cggacgaata aagataaaat
     3901 ggcgacgatc catttgacgg ttggtatcca gaatttatcc cacctaaaga gtatcgtgga
     3961 taaaatcaaa gcagtacctg atgtctacag tgtacgccgg acgaatggat aggaggacag
     4021 atatgagagc agttgttcaa cgggtaagta aagctgctgt gtcagtcgat cagcaagaaa
     4081 ttggaaaaat tggctacggc ctacttgttt tattaggcat tcatgaaacc gatacgcaaa
     4141 aagatgtgga ctatctggta aaaaaaattg cacaaatgcg tatttttgaa gatgaacaag
     4201 gaaaaatgaa tattagtgta gaagatgtgg gaggacaaat tttaagtatc tctcaattca
     4261 cactgtttgc ggatacgaaa aaaggaaatc gaccaagctt tgtggaagcc gcacgaccag
     4321 aaacagctat tcctttatac gaatcattca atgaaggaat cagaagtcgc ggcatcacag
     4381 tcgctaccgg acaattcgga gcagatatgt cggtttctct cgtcaatgat ggacctgtaa
     4441 cgatcattat tgattctaaa aacaaataaa gtaatctggt tttcactagt aaaaactatg
     4501 aaacacgtcg cttaa
//
LOCUS       DACEUF010000123         4489 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.129, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000123 DACEUF010000000
VERSION     DACEUF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.129"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(207..1169)
                     /locus_tag="IXG20_002497"
     CDS             complement(207..1169)
                     /locus_tag="IXG20_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1610660.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IXG20_002498"
     CDS             complement(1171..2610)
                     /locus_tag="IXG20_002498"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ1610661.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4216
                     /locus_tag="IXG20_002499"
                     /pseudo
     CDS             2795..4216
                     /locus_tag="IXG20_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
     gene            4349..>4489
                     /locus_tag="IXG20_002500"
     CDS             4349..>4489
                     /locus_tag="IXG20_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1610662.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKY"
BASE COUNT         1419 a          782 c          737 g         1551 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatgagagt
     4261 gtaaaatatt ttgtgtaaat agaaaaaagg aagtcccttc tgtagaatag agttaccaca
     4321 acacattcac agaaaagagg acttccatat gaacgatttt actacagaaa ttctaaagac
     4381 tctagcgaac aaaggcgatt tgaatgaatt attccgtgtc catttggaaa aagctgtcaa
     4441 tacgcttctc aaaacggagt taacggcttt cctcgattac gaaaagtac
//
LOCUS       DACEUF010000124         4374 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.130, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000124 DACEUF010000000
VERSION     DACEUF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.130"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(83..1051)
                     /locus_tag="IXG20_002501"
     CDS             complement(83..1051)
                     /locus_tag="IXG20_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1610663.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2904)
                     /locus_tag="IXG20_002502"
     CDS             complement(1498..2904)
                     /locus_tag="IXG20_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1610664.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3126..3488)
                     /locus_tag="IXG20_002503"
     CDS             complement(3126..3488)
                     /locus_tag="IXG20_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610665.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3492..3800)
                     /locus_tag="IXG20_002504"
     CDS             complement(3492..3800)
                     /locus_tag="IXG20_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610666.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3809..4228)
                     /locus_tag="IXG20_002505"
     CDS             complement(3809..4228)
                     /locus_tag="IXG20_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610667.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4221..4361)
                     /locus_tag="IXG20_002506"
     CDS             complement(4221..4361)
                     /locus_tag="IXG20_002506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610668.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1234 a          994 c          619 g         1527 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tagaaatata
     2101 cctcaactca tcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat
     2161 ttcactcatg atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc
     2221 ccttttccaa ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa
     2281 attattgaaa agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc
     2341 tttattaaag ctcggctgct tttgaacacc ccgtttataa ccttcagcaa ccggcacaat
     2401 attcaagtgc aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa
     2461 attagggttt ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac
     2521 tcgattttgt tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt
     2581 ctggaaatca tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt
     2641 atccttgcgc actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc
     2701 attatatttt tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg
     2761 ctctaaataa atattttcat ccgatttcgt gaagtcaata tgcttatgcc aatcattggt
     2821 tttctggcga tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt
     2881 tttaaatgaa atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag
     2941 attgtaaacc tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc
     3001 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     3061 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     3121 atcgcttatc gtaaattcac gtcattaaat attgacgtaa atttactctc actcatctga
     3181 cttttcttat tcggtatcac tccgaataag ccgtcagatt catcgggata atctcgttcg
     3241 cttggcgctg atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga
     3301 tcgtcagaaa tcgacgatag ccaccgctca aaatcagcaa aagtttcaaa cccaaaacgc
     3361 ttgcgaagag ccttgcccat tgcagcattt tctttctcaa tcaagcgttt tttctgttgg
     3421 cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg ctttaatatc tgctaaagtc
     3481 attcccataa actacatctc cttttctttg gaattaataa cggaaatttg gtttaactgc
     3541 ataacatgac tcattaattt caaccaaaat tctttttctg attcatcagt tgtttgcgct
     3601 aaatgcttat gaacaaattc attagtcaaa actgaagatt ttgctagatc agcattgata
     3661 accgagttga gaacatccat cattcgttca agcatttcac tataattatc ttcatccggc
     3721 aaattcaaat aatcagctaa ccgttcatac atcaattcaa ttctatcgac tgtttcttct
     3781 ctcaaatcaa ttttatccat gtcacacctc acttccagaa ttgccaccat tttcgatttt
     3841 ttaattcttg attttctagt aacaactgat cgtttttttg ttgctgaagc aaagtcaatt
     3901 tttgttggtc acgaatcatt tccgttaatt cagaaatatg cctatctttt tccgctaatt
     3961 gttcacgcaa aaattcattt tctttttcta acgggaaatc attgcctgca tcattgaccg
     4021 gtaaatcacc agtcaattga ccaccttttg accgataaat tgaccgcaca acaccaatca
     4081 attccttgtc taacatgata accccattat ctttataccg agcaaggtca taacctgcat
     4141 tgaccagttg attgcgcact gtatttttag atatacccaa ctcatcagca aggtttttta
     4201 tggtcatttt ttctggcaaa tcagtcatta tcttctccgt tactttcagc taccccataa
     4261 acatatttaa cagcatgctt agggataatg attggggaat caattttatt atttttcaaa
     4321 atcaactcat actgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DACEUF010000125         4324 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.131, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000125 DACEUF010000000
VERSION     DACEUF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.131"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(1..657)
                     /locus_tag="IXG20_002507"
                     /pseudo
     CDS             complement(1..657)
                     /locus_tag="IXG20_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(662..1429)
                     /locus_tag="IXG20_002508"
     CDS             complement(662..1429)
                     /locus_tag="IXG20_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1610669.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1918..2343
                     /gene="lacA"
                     /locus_tag="IXG20_002509"
     CDS             1918..2343
                     /gene="lacA"
                     /locus_tag="IXG20_002509"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ1610670.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2360..2875
                     /gene="lacB"
                     /locus_tag="IXG20_002510"
     CDS             2360..2875
                     /gene="lacB"
                     /locus_tag="IXG20_002510"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ1610671.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2886..3818
                     /gene="lacC"
                     /locus_tag="IXG20_002511"
     CDS             2886..3818
                     /gene="lacC"
                     /locus_tag="IXG20_002511"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ1610672.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTVSHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3821..>4324
                     /locus_tag="IXG20_002512"
     CDS             3821..>4324
                     /locus_tag="IXG20_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HAQ1610673.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYD"
BASE COUNT         1431 a          681 c          845 g         1367 t
ORIGIN      
        1 gcttatgacg acttttggaa gtaaaaatcc atccattatc tttataatca ggattgaatc
       61 ttatatgttg gtgatttaac tcaatttcct ctttaagaat ttctatagcc cttttagata
      121 attggacatt ttggtaactt tgtaagtttt ttacattacc ttcatagcta ttttctgcag
      181 ctcctccgtg agtggactga ctaatgatga tattttgctt ttcaaaatat tgataggcac
      241 caatattttg gagttttcga ctctgtaagt caacagggcc attagttaga atacctattg
      301 ggatgcggtt tttatgtaga gccagtagta gttgatgtat ctcaggaatc atcgtaatct
      361 gatcgagttc atattcatag tcagcttgga agtctagagc ttctgtccta gaaatactat
      421 cgatgccaaa ttcttccata gtcttctgac agcgaaaaat tctaagctgc tcaatactga
      481 tgagtttttc gtgtatttag gaaaagcaac atcagaccaa catctgaaac gtttgtaaat
      541 gttatctatt tgttggatat caatcgttgg aaaacatttc tcaacgctac ttttaaaggg
      601 gagttgatgg tcataaagag tgtcatcaag gtcaaaaaca atatttttat aagtcataat
      661 tctagtccgc ttttaaaata gtagtgtact ggctaaattc ctctaagtgt tgagggctaa
      721 tctggttatc tgtaatgatg gtatcgagtt gatctagatt atagaagtta aagaaatcgt
      781 atcgatcgaa tttagtactg tctactaata agaatttttc tacagcattg tttagggcaa
      841 gttgttgaat cacaccttcc ttgtcactat atgtagcaat agaattatgg gtaacagcat
      901 ttgcactaac aaaagctttg gcaaatctaa ttgcttttaa atttgtcgaa gccattgaac
      961 ctacaaaagc tccagttatt tctctatatt caccgccaag aagcaataaa tcaatggttt
     1021 cgctatcatt tagaatcaag aacacaggga gactatttgt aatgacacga attttataac
     1081 ctttacgacc tttcaattct aatgccagtt gttcaagtgt agttccgggt ccaataaaaa
     1141 tagtatcgcc atctttgatt aaagagcagg cttttttagc aatgtcaatt ttttcttttg
     1201 tatttagact tttcttctcg atatgtgtct tttcaagtgg ctttttagag gaaagtaatt
     1261 gtgcaccacc atgagtacgt gttaaaagtc catcagcttc tagagcatca aggtctctac
     1321 gggctgtcat atcggaaata tctagttcat ctattatttc ttttatggtt atggtcccat
     1381 caatctttaa catatctaaa attttttcta atcgtcgttt tttgttcata tgaagaccct
     1441 tctttcataa agtaattttt ttccaaagat aattctcttt taattgtatc ataaaagaca
     1501 atattttcaa ggtaaaacaa acaatttcaa acaaaaacaa atgttagatg atgaaataag
     1561 aacagtggat tgacgtatat tagcttaggt cagattttgt ataagacgaa aataaagtag
     1621 gacctcttaa tcagtaagtt atagaaagta aaagactttt gtaatacttg aatagatatt
     1681 tcacgtccat tttgtgatgg attaaatgaa caaaaatgaa caataattta acggtgttat
     1741 ctatttttta aaaaaacaaa taaaaaaaca aaaaataaac aaaaaaagtg ttgctttttg
     1801 tttgaatgct tgatattata taaacaaaga aatgatgaaa acgttttttc gaacattttg
     1861 caaaatgttt tctacttcta cgtagcattt ctttctaaaa tttaggaggt ggtcaaaatg
     1921 gctattgttg ttggtgcaga tctcaaaggt acgagattaa aagatgttgt gaaaaatttt
     1981 cttgtagaag aaggttttga ggtaattgat gtgactaagg acggacaaga ttttgttgat
     2041 gttaccttgg cagttgcctc tgaagtaaat aaagatgagc aaaacttagg tattgtaatt
     2101 gatgcttatg gagctggtcc attcatggta gcaactaaaa ttaaaggtat ggttgcagca
     2161 gaagtttcag atgaacgctc agcttatatg acacgtggac ataacaattc acgaatgatt
     2221 acggttggcg ctgaaatcgt cggtgatgag cttgctaaaa acatcgctaa aggtttcgtc
     2281 aatggtaaat atgatggcgg acgtcatcaa atccgagtag atatgcttaa caaaatgtgt
     2341 taaggaaagg agttaaggaa tgagaatcgc aattggatgt gaccacattg tcacagatga
     2401 aaaaatggca gtatcagaat tcttgaaatc aaaaggacat gaggtccttg atttcgggac
     2461 atatgaccac gcacgtactc actaccctat ctatggtaaa aaagtcggtg aagcagtagt
     2521 aagtggtcaa gcagacttgg gagtatgtat ctgtggtaca ggtgtcggca ttaacaatgc
     2581 agtcaataaa gttccaggtg ttcgctcagc gttggttcgt gatatgacat cagctatata
     2641 tgcaaaagaa gagttaaatg cgaatgttat cggtttcggt ggaaagatta ctggtggtct
     2701 tcttatgaat gacatcattg aagctttcat tgaagctgag tacaaaccaa cagaagaaaa
     2761 taaaaaattg attgcaaaaa tcgaacacgt tgaaacacat aatgcacatc aagcagatga
     2821 agatttcttt acagaattcc ttgaaagatg ggaccgtggt gactaccacg attaaggtat
     2881 aacaaatgat tctgacagtc acactaaatc cttcagtaga catctcttat cctctagaaa
     2941 cattgaaaat tgatacagtg aatagggtga aagatgttag taagacagct ggtggaaaag
     3001 gcttaaatgt tactagagtc ttgtatgaat ctggtgataa agttactgct acaggtttct
     3061 taggtggtaa aattggtgaa tttattgaaa gcgaattaga acaatctcct gttagtccag
     3121 ctttttataa aatctcagga aacactagaa actgtattgc tatcttacac gagggtaacc
     3181 aaacagaaat tttggaacaa ggaccaacag tttctcatga agaagcagaa ggttttcttg
     3241 accactatag caatcttatt aaacaatcag aagttgtgac aatttcagga agtttacctt
     3301 caggacttcc caacgattat tatgaaaaac ttatccaact tgcttcggat gaaggagtag
     3361 cagtcgtttt ggattgttca ggtgcacctc ttgaaacagt tctaaaatcg tcggctaaac
     3421 ctactgccat caaacctaat aatgaggaac tttcacagtt gcttggaaaa gaagtaacaa
     3481 aagatattga agaactcaaa gacgttctta aagagtcact ttttagtggt atcgaatgga
     3541 tagtggtctc attaggtcga aacggtgcct ttgcaaaaca tggtgatgtt ttctataagg
     3601 tagatattcc tgatatccca gttgttaatc cagtcggatc aggggactca acggtagctg
     3661 gtattgcatc agctttaaat agtaaaaaaa gtgacgctga cttattaaaa catgcgatga
     3721 cattgggtat gttaaatgct caagaaacaa tgacagggca tgtgaatatg actaattacg
     3781 aaacactaaa ctcacaaatt ggagtaaaag aggtataaaa atggtactta cagaacagaa
     3841 acgcaaatct ttggaaaaaa tttcagataa aaacggtgtt atctcagctt tggcatttga
     3901 ccaacgtggt gctttgaaac gtttgatggc acagtatcaa gatacagaac caactgtagc
     3961 tcaaatggaa gaactaaagg ttttggttgc tgatgaactt acaaaatacg cttcttcaat
     4021 gttgcttgac cctgaatatg gacttccagc aacaaaagca ttggataaag aagcaggtct
     4081 tcttcttgcc tatgaaaaaa caggttatga tacatcaagt acaaaacgtt tgcctgactg
     4141 cttggatgtt tggtctgcaa aacgtatcaa agagcaaggc gctgatgccg ttaagttctt
     4201 gctttactat gatgtagaca gctcagatga acttaaccaa caaaaacaag cttatattga
     4261 acgtgttggt tctgaatgtg tcgctgaaga tattcctttc ttcttggaaa tccttgctta
     4321 tgat
//
LOCUS       DACEUF010000126         4266 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.132, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000126 DACEUF010000000
VERSION     DACEUF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.132"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..1485
                     /locus_tag="IXG20_002513"
     CDS             <1..1485
                     /locus_tag="IXG20_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1610674.1"
                     /translation="CEAKFSENSYGFRPNRSVENAIKATYNRLQISQLHYVIEFDIKG
                     FFDNVNHSKLIKQIWAMGIRDKHLIFILKRILKAPIKMTNGTITYPEKGTPQGGIISP
                     LLANIVLNELDHWVESQWQENPVTKNYVVHINKSGSPCKSNAYKEMKKTKLKEMYMVR
                     YADDFRVFCRYKESAEKAKIAITQWIEQRLKLEVSQEKTRIVNVRKRYSDFLGFKIKM
                     IPRRKKLVVKSHISDKQFKNQKDKLVSQAKKIASPSKGKTELEETRLYNSMVLGMQNY
                     YQIATNVNLDCMKLNRAVMAIFTNRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYV
                     AGEGEPIFPVGYIQHRKPMAKVYLANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENR
                     TIEYADNRISLFSAQHGKCAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAV
                     HRLIHAKTEETIQKYIALLKLNKVQLIRLNKYRVLAGNQELNLI"
     gene            1589..1813
                     /locus_tag="IXG20_002514"
     CDS             1589..1813
                     /locus_tag="IXG20_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610675.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            1826..2329
                     /locus_tag="IXG20_002515"
     CDS             1826..2329
                     /locus_tag="IXG20_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1610676.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            2389..2778
                     /locus_tag="IXG20_002516"
     CDS             2389..2778
                     /locus_tag="IXG20_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1610677.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            2765..>4266
                     /locus_tag="IXG20_002517"
     CDS             2765..>4266
                     /locus_tag="IXG20_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1610678.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT         1427 a          696 c          919 g         1224 t
ORIGIN      
        1 tgcgaagcta aatttagtga aaacagctat ggttttagac ctaataggtc agttgaaaat
       61 gcaataaaag caacctataa ccgcctacaa atcagccagc tacattatgt gattgaattt
      121 gatattaagg gtttctttga caacgtgaac cattccaagc tgataaaaca gatatgggct
      181 atgggaatac gggacaagca cttgatattc attttaaaaa ggatattaaa agctccaatc
      241 aaaatgacta acggtacaat aacatatcca gagaaaggaa ctccgcaagg gggaataata
      301 tccccactac tagcaaacat tgttcttaat gagttagacc attgggtaga aagccaatgg
      361 caggaaaatc ctgttacaaa aaactatgtg gttcatatca ataaaagcgg tagcccatgt
      421 aaaagcaacg cttacaaaga aatgaagaaa acaaagttaa aggaaatgta tatggttcgt
      481 tatgcagatg attttagagt tttctgccgc tacaaggaaa gtgctgaaaa agccaaaata
      541 gcaatcactc aatggattga acaacgatta aaattggaag tatcacaaga aaaaacaaga
      601 attgtaaatg ttagaaaaag atactccgac tttcttggtt tcaagattaa aatgataccg
      661 agaagaaaga agttagttgt taaatcacat ataagtgata aacaattcaa aaaccaaaaa
      721 gacaagctgg tatcacaggc aaagaaaatt gcttctccct caaaagggaa aacggaatta
      781 gaagaaacaa gactttataa ttcaatggta ttaggtatgc aaaattatta tcagattgca
      841 acgaatgtaa atctggattg tatgaaactg aatagagctg taatggctat cttcactaac
      901 agattaggta caaacaaaag gggcagattg agaaaaacag gcagacaact tacgacaaag
      961 gaggcaacaa gatatggtaa atctaaaatg cttcggtatg ttgctggcga gggcgaacct
     1021 attttccctg ttggatatat ccaacacaga aaaccaatgg caaaggtgta tttagcgaat
     1081 tgctacactc tagagggaag aaaatttatc cacaccaatc taagtgttga taaattactg
     1141 atgtaccagt taatgaaatt atctttggaa aatcgcacaa tagaatacgc agacaatcga
     1201 atttcgctat tttctgctca acatggaaaa tgtgctataa ctctggaaga atttcaacaa
     1261 gctaatgaaa tacattgtca tcatattgta ccaactggag ctggtggaaa tgacgattat
     1321 aaaaatctta ttttggtaaa agaggcggta caccgtctaa tacacgctaa aacagaagaa
     1381 acaatacaaa aatatatagc tctattgaag ctaaataaag tccagctcat aagactaaat
     1441 aaatatagag tattagctgg caatcaagaa ttaaacttga tataggtcta ctacataaat
     1501 acttttcaat ataaacacga tggaacgcgg agtgcggtga aagtcgcacg ctccgtgtga
     1561 atggggggaa aagtttgaga tatttataat atcaggaact tacctatcca tatatcaatg
     1621 gtttttaacc aatgcccaat ccttagtctt actggcaatt gtggtcattg gtttgttttt
     1681 aggatttaag cgggagtttt ccaaactgat tggctttttg gtgattgctt tgattgcggt
     1741 cggattggtc tttaatgctt ctggggtgaa ggatgtctta ctcagtttgt ttaaccgaat
     1801 tgtcggtgcg taagggggaa gcacgatgga acagatgcgt gtttatattg cgaacctcgg
     1861 gaaatataac gaaggtgaat tggtgggggc gtggtttacc ccacctgtcg attttgatga
     1921 agtgaaagaa cgaattgggt taaatgacga atacgaggaa tatgcaattc atgattatga
     1981 acttcccttt gagattgatg agtatacgcc aattgaggaa attaatcggt tgtgtggctt
     2041 agcggaggaa cttgaaggaa cgccagttgg tgaagttgct tctgagattc aacatgcttt
     2101 cttcaattct tttgaggaga tggtagagca tgtggatgat attatctatt atccagattg
     2161 cgatgatatg agtgatgtag catatttctt gattgtagaa gctggtgatt ttggagaagt
     2221 accagaacac ttgaagacgt atattgattt tgatgcgtgt ggtagagact tggagattgg
     2281 aggcaattat ttggtaacga gtcgaggtgt ttttgagtat ccatattaac aaaattattg
     2341 aagggtagtt gatttctaaa ctatccttct ttttaaagga atgatttgat gaagaaaata
     2401 aaaagctaca ccagtatttg ggcagttgaa aaggtcattt atgcaattaa tgactttcaa
     2461 ctgccttttc ctgtgacttt taaccagatg gcttggtttg ttttatcctt gctgtttgtc
     2521 atcgtatttg cccatgtgcc tccactatct atgatagagg gggccttttt aaaatactta
     2581 ggtataccgg tggcagttac ttggttcatg tcgcaaaaaa cgtttgacgg taagaaacca
     2641 ttgggatttt tacgatcttt tattagctat cacttacgat ttaaggtcac tttcgcagga
     2701 aagaaagtca aagaacagaa gaaacgatgg gacgaaccca ttactctggt aaggagtgtg
     2761 aactatgtac ccgattaagt atattgaaaa taatcttgtc tttaatcaag agggagaatg
     2821 ttttgcctac tatgagttaa taccttacaa ttactccttt ttgtcacctg aacagaaata
     2881 tcaagtccac gataactttc gtcaattgat tgcgcaaaat cgtgaaggca agattcatgc
     2941 cttacaaatt gccacagaaa gtagtattcg tgccacgcaa gaacgatcaa aaaaggaaat
     3001 taccgggcga ttaacagaag tagcgaaaca acgaattgat ttacagacag aggcattagt
     3061 atccatgatt ggcgatagtc agattgatta tcgctttttt attggcttca aactgattgc
     3121 gacagatgaa gaggtcaatc taaagaatct caagaaatct ttcttctccg gctttcaaga
     3181 atttgtctat ggagtgaatc atcatttgat gggcgatttt gtttccttat ccaatgaaga
     3241 aattcgccga tataccaagc ttgaaaaact aatggaaagt aaattggctc gaagatttaa
     3301 agtcagacga gtcacaccga gtgatctgat gtatttgatt gaacatatct atggcgaaaa
     3361 gggtacacca tttgaagaat atgaatttca gctaccaaag aaaaagctga agtcggaaac
     3421 tttggtgaaa cgttacgact tattacgtcc cagtcgttgt ttgattgaag aaaagccccg
     3481 gtacttatgc atggaacatg agaatcatga atcatatgta gcgtacttaa caattaatac
     3541 aattgtgggg gaaatggagt ttccctcctc agaacttttc tattaccagc aacaacaatt
     3601 tacattccca attgacacta gtatgaatgt ggagattgtg acgaacaaaa aggcacttgc
     3661 taccgttcgc aataagaaaa aggaattaaa agacttggac aaccatgcct atcaatctga
     3721 caatgaaaca aattccaatg tcttagatgc attggattcc gtagatgaat tggaaactac
     3781 cttggatcag tccaaagaat ccatgtacaa actgagctat gtggttcgag taagtgccga
     3841 atcagtagat gaactaaagc gccggtgtga tgaagtgcta gatttttatg atgataccaa
     3901 tgtaaaacta gtccggcctt ttggcgatat gatggggcta catgaagaat tcttaccttc
     3961 tagtaagcga tacatgaatg attatatcca gtatgttacg tctgattttc tcgccggact
     4021 tggttttggc gccactcaga tgttagggga gttggaaggc atttactttg gttataacgt
     4081 agatacgggg cgcaatgttt acctgaaacc ggcattggcc tcacaagggg tcaagggttc
     4141 agttaccaat gcgctagcag cggcattcct cggttcactc ggtggcggaa agtcctttag
     4201 taacaatctc ttagtttatt acgccgtcct ttttggtgga caagctgtga ttgtcgatcc
     4261 aaaagg
//
LOCUS       DACEUF010000127         4226 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.133, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000127 DACEUF010000000
VERSION     DACEUF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.133"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..345
                     /locus_tag="IXG20_002518"
     CDS             <1..345
                     /locus_tag="IXG20_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610679.1"
                     /translation="SNIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQD
                     KKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQK
                     KRDNNDQYLPKK"
     gene            426..1046
                     /locus_tag="IXG20_002519"
     CDS             426..1046
                     /locus_tag="IXG20_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610680.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     EIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQIS"
     gene            complement(1376..2602)
                     /locus_tag="IXG20_002520"
     CDS             complement(1376..2602)
                     /locus_tag="IXG20_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1610681.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2674..2832)
                     /locus_tag="IXG20_002521"
     CDS             complement(2674..2832)
                     /locus_tag="IXG20_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1610682.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3520..>4226
                     /locus_tag="IXG20_002522"
     CDS             3520..>4226
                     /locus_tag="IXG20_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1610683.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIG"
BASE COUNT         1449 a          591 c          691 g         1495 t
ORIGIN      
        1 tcaaatataa aaactagtac gaaattaatt tgtaaattat ttaagcttga taaaataagc
       61 aatatcgatg atgtaaagtc caagatatta acaagaaaag taagcgaacc agtaaagcta
      121 acggcacaag ataaaaaaaa tggaattgtt ctttatggtg atgatgcaaa gtataactat
      181 aattgggata cctcctcaga agctgaggta ttaagtattc ctactaccga tacttcaggg
      241 gaaaaagtca cctttaaaat aaattttata acggcaattt ctaagctgga gtatgggaag
      301 caaaaaaaga gagataacaa tgatcaatat ctacctaaaa aataacgaat taatgatgca
      361 tatgttaaag ttgttttttt gaattaaaca actcaattta ttcagttata tgttcatcgc
      421 agcaagtgaa cattaatcga gttataaaat taataggagc taaaaatata aataaagttc
      481 gatcagaatt tatttttcca gaagattttt ttaactggtt tttttatcaa ttcagtgaat
      541 gcaagcgact cttaggtgag aattattact taaaaggaat cggcggattt atggggaatg
      601 tggctgatga acaaaatatt ataaaaggaa tatctgaaca aacatcagag cttacagtaa
      661 ctaaagcatt tataagtaat ggtgaaagtt tgaaaaatgt aactgcgcga attagaaaag
      721 aaaatgaagt agaaattgtt tttggaattg accatgaatg taattcagtg atatatgtaa
      781 cacaatcagc aaatttaatt ctttttgaga atggttgcaa ggacgatttt atggtggtct
      841 atttgtacga tatactaatt ccaaaacttc gttccttata tatgtcttct gaaagtattt
      901 ttgtgagtga acaaaaggga tctttctcga aaaaaatagg tattgaagta attgaatcga
      961 ttatgtgtca taatcatatt acgctagaag aattagaaat tttgataggg caaaaaaata
     1021 ataacacgaa aatacaaatt tcttagaaat cattttttat tgagatatct agataaatga
     1081 tttattcttc aatcaaccaa ctcgccaagc tgaggtaatg ttttgaggta atgataacat
     1141 acaacttttt ataccctagt aaaactctat aaacaaagac ttattaattt cgcatgaaat
     1201 acattcctgt aaaatgcaat aaaatagagt tttgtttaac caggtctcaa aaaggctcgt
     1261 aaagcatctc aattctctaa acgttaatat tatttatata tcaatgtttc aaggcacttt
     1321 tcaattgttt gaaaagtgtc tttttcttgt ttaagaacac atttaagaac acatactaat
     1381 cacatttatt caatcctctc tgaaaggctt caaaaacagt tttatctact acatctggtg
     1441 tatttacgta tattctcgtt gtagaaatat ctgagtgtgt taaagcacta gatatttgat
     1501 tcatatttgc tccgccttgt ctagctatag ttgcataggt atgacgaagc ttatgtggag
     1561 ataaatgagc caattcagga tgtcgtcttt taattgaatt gattcgatag tttaggtaat
     1621 cagcgtgaac agggcaattt acttctccgg atggtttagt ataggtaaag agatactgat
     1681 ctggtgtttg attaattttt aatctcaaca attgctcagc ttgcactgat ttccatttgc
     1741 ttagtaatgc cattacaatt tcgggtactt gaattacggt atcctttcga ccttttgtaa
     1801 atttcttttt ttgatattta tccaaagcat gtttaaggcg aaccgtttgt ttctcaaagt
     1861 cgatatattt ccattgcaaa gcatacgtct cactttttct atcaccaaga tatagggtaa
     1921 gcatgaaaag agtatagtca tgaagagtaa gcaagtgatt attcaagtca gtgtttactg
     1981 cttctatcca agcaataagc tcttctgacg ataatgcttg attaccttcc cgtatccttc
     2041 tttcttctaa agcaattttt ttaggtgcag tgatacattg aataattttg gtagttcggt
     2101 taaattcaat ataatttagt atttctgcaa tatcaaacat agaacggaca tatcctttga
     2161 taatttttat atttgcgtat tctttcgatt tttttgttaa ttcatctgat attatctcag
     2221 tatttgaatt tagatagttc attgcatact caccaaacat tggaaggatg tgcaatcgga
     2281 atatatcttt agtattacta attgttacgt aacttggagc tctttttgat ctagtagtct
     2341 gacctagttc atatctagga agccattttg attcatagaa ttccttaaat tttatttttc
     2401 cttttaattc tagtgaattt gcattctcct ctcttaaaac tgtttcaatt ttccttttca
     2461 ttgcattttc ggctttaata gccaattgcc tagttggaaa tactttttga tacttttctg
     2521 atgtaattcc agtgatttct ctgaattttt ttggatacct cagattagtt ttatatttcc
     2581 catttgaaag ttttgtaggc aaaattcctc actcccttaa aaagtaaggc agtatatttc
     2641 gctattctta gtatatgcgg cgagaagaaa ttttcaaatt gttttaaatg tattcataaa
     2701 atctttgata tcctctatgt caaagcgaac tataccgtca attctgatta ctggaaaatt
     2761 atatatacga atccacttat ctaaagtatt attatcaatt cctagaaact cacaggtttg
     2821 ttttttattc aagtagactc tcaattttat aaaatcatct cctaaaaaag attagaattt
     2881 ttgaattgct ttgggtaagc acataggaat ttcacctctc cccagtttac ggcgagcctt
     2941 acggaagtat cattatttgc tgctattgtc atggcatttt gttttcccag aacaaaaaga
     3001 aatctctaag tatttgtcgc taatcaggtc atggcgtact tgagtttcta gctcgaatag
     3061 cagggatcgt tattcaaaaa ttgtatttaa ttgtcaatga tcaaaaatag gttttttcct
     3121 attcactatt aataacgata aacatgtata aaaagacaca ctacgaaaaa agttaaatgg
     3181 agtttactga ttttcatatt tgtatatgga acagacatag tcttgaataa tttttataaa
     3241 tgttttgatg cttatcaact tattcaaccc gctatccact ttaagtataa tttttccata
     3301 cgccaataaa gtagttttag gagcatgttc tcatttgaga acgctccttt tttatatatt
     3361 ttgaaagcta cttactatac gcgtagtttc aaatagtttt gtcattctaa tcttcagcaa
     3421 cagaaaaaaa tctaaattat aaaatttagg gttgactaaa atataatttt tgtataccat
     3481 atgatcgtga taatcattat catttagaag gagaaagtta tgttagaagt gaaaaagctt
     3541 aatgtttgtt ataacgattt tttagcattg aacgagattt ctttaaaaat acaagaaggt
     3601 gctttaacag gtattattgg tccaaatggt gctggaaaat ctacattgct taaagctatg
     3661 ttaaatatta tccctcatca gggagaggtc tccattaata atgaagatat caataaaaaa
     3721 ctggtgaaga ttgcatatgt agagcaaaaa gctgatattg attttacttt tccaataaaa
     3781 gtaaaagaat gtgtatctat gggaacatat gccggaatga aagtttttca aagaattaaa
     3841 aatgcagaat ggcaaagagt ttcaaaggct ttggaaaagg tcgatatggg aaaatattct
     3901 aatcatcaaa taggagaatt atctggagga caatttcaac gtgtactttt agcaagatgt
     3961 cttgctcaaa atgcggattt catattttta gatgaaccat ttgtagggat agacttagtt
     4021 agtgaacgaa taattatgga tacgttaaaa gaattgaaac atcagggaaa aacagtgcta
     4081 attgtgcatc atgatttaag caaggtaaag aagtattttg ataatattat tcttttgaat
     4141 cgaaatctaa ttgctcacgg aagtgtagaa tctgtattta atgaagagaa tttaaaaaaa
     4201 gcctatggag atactatatt tattgg
//
LOCUS       DACEUF010000128         4216 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.134, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000128 DACEUF010000000
VERSION     DACEUF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.134"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            624..3263
                     /locus_tag="IXG20_002523"
     CDS             624..3263
                     /locus_tag="IXG20_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1610684.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3431..4129)
                     /locus_tag="IXG20_002524"
     CDS             complement(3431..4129)
                     /locus_tag="IXG20_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1610685.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIAAILLIMIPI
                     ILFATTIYDGSASVSDATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVIGLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT         1277 a          805 c          912 g         1222 t
ORIGIN      
        1 ttctttctgt tctgtccaaa ttattctaag atttctcttt accaacatca tttgacaaag
       61 agccagaaaa agccgagctc tcgatgagct cggctttctt gtacctcgga aggattcaaa
      121 taaacagaaa tgattgtaac tttccatcat acatgcaaag cacggtgttg aatttcttca
      181 tgttaaacaa aaattttgtt ttcgttacat cttcaacatt tgaaacatct cttgttccta
      241 ttcaattttc agacgtttac gttattcaat tgttacgtat cgcattactt cttgtttcgg
      301 atacttcgtg atccaaacat ccatacgcat tcactattta taggtttgta ctctacacgc
      361 atgtctgggt gaaacctgta cttctaacaa ctttatttgc ttacactgtt ctatcgagaa
      421 attcttctgt ctatttgatg gtatcctcct tccttacatc tatataatag catcctttgg
      481 tagcgtttgc aaatataagc actttaaatt ttacgctttc ttaacaaata atcgataagc
      541 cctttatttg aagtaaataa aaaaaggatg ttatagtaaa ataagtacct ttttttgtac
      601 gaagatttta aaggaggaat aggatggaag aatacaagca gacaatagaa gaagtaaaga
      661 aggtaacaga agcagatcca gatactgggt tgtcttcaca agaagtggat gaacggcgaa
      721 aaaagcaggg attgaataaa ttcgatgagg cgccgaaaga atcgatgatc aaaaaattct
      781 ttcgaagctt atccgatttt acaacgatca tccttcttgt agcagcggtg atttcctttt
      841 ataccgcatt tgctacagaa catggggatt tatttgaagg actgctgatc atcgctatcg
      901 ttgtgatcaa ctcggtattg gcgattgtcc aagaaggaaa cgcagaaaaa gcactggaat
      961 ccttgcaaga tatgaataaa caaacggcaa ctgtcatacg agacggcaaa gtgaccactg
     1021 ttgagtcaga gcaactggta gtgggtgata ttcttgtttt agagtccggt gatgctattt
     1081 ctgccgatgc ccgtttgatc gaagcatcac aattacgtgt agaagaatcg gcgttgactg
     1141 gagaaagtga agcagttgaa aaagatgcag catttattgc tgaagaagat gagtcattag
     1201 gtgaccaact taatatggta tttaaaggat gtacggttgc tgcaggtcgt gggaaagcga
     1261 ttgtcactgc cattggtatg gctacagaaa tgggtaagat cgcggatctt ttgaacgaaa
     1321 atactatgca aaaaacacct ttacaggtcc gattgaatca gctaggcaaa cgaatcagta
     1381 tgatcgcttt agcggctgcg gctttagtat ttgtgattgg tgaactgcaa ggagaaccac
     1441 tccttgagat gttcatgact tcgatttcct tggctgttgc agctgtaccg gaaaccttga
     1501 ctgttattgt tacgttgaca cttgcatacg gtgtgcagaa gatggcgagg aaacatgcga
     1561 tcatccgaca gttgccagca gttgaaacgc taggaaccgc taatgttatc tgctcagaca
     1621 aaacaggaac gctgacacaa aatgaaatgc gcgtccgccg ggtatggagc aaagaagatg
     1681 aagtaacgaa tatcgaagat tcaatgacga acagcgcgat ggaaatattg aaaatggctg
     1741 cactatgtac agatgtgacg gtcgagcaag aagatgacga ccttgttatc aaaggtaatc
     1801 caacagaagt agccatcgta agggctgttg aagagaatta tcatacaaaa gcagaattag
     1861 aagaaaaata tccacgagta aacgagcttc cttttgattc tgaacgaaaa atgatgacaa
     1921 cagttcacca aatgggcgaa aaatacatct caatcaccaa aggagcattt gatgttcttg
     1981 ctccgcgctt tagttcaggc gatgtagaac aagcggcgat cgttaatgat cgctttggta
     2041 aacgggcact gagagtgatt gctgttggtt atgctactta tgacgaagaa ccgcaagata
     2101 tttcttctga agcactggaa aaagacttac gtttgattgg tctgatcgga atgatcgatc
     2161 caccacgtcc agaaagtaaa ggagcaatca agcgagcgaa aaaggctggc atcaaaacag
     2221 tcatgatcac tggagatcat gtggtaactg ccagtgccat agcgaaagaa ttaggtatct
     2281 tgaatgatcc gtcagaagca ctctcaggat cagaactgca tcaattatca gacgaagaat
     2341 tagatgcgcg tgtcaaagct ctctctgtct atgctcgggt aacgccagaa gacaagatcc
     2401 ggatcgtcaa atcttggcaa cgtacgggtg cagtcgtagc gatgactggg gatggtgtga
     2461 atgatgcacc agcgttgaaa gcaagtaacg ttgggtgtgc catggggatc acagggacag
     2521 atgtggcgca aagtgccgca gatatgattt tgacggatga caactttgcg acgatcgtag
     2581 atgctgttag tcaagggcgt tcagtttatc agaatattcg taaagcaatc aactttttac
     2641 taagctgtaa tatttcggag atctttattg ttttgattgc gatgctgtta ggatggggag
     2701 caccatttac agctgtgcaa ctactgtttg tcaatgtagt tgctgatgga cttccaggat
     2761 ttgctttagg aagagagcca gcggaacacg gcatcatgga tcagccaccc attccgaaaa
     2821 atgaagggat ctttgctcga ggtttattgc aaaaaattgc cataaatgca ggggtcttca
     2881 ccatcgtaac cttgtttggt tattatttag gtagttatgt cgatactatc agcccatggg
     2941 tagacgcaag tcagcatgtc ggtcaaactg ttgcattctt gatcttagct tattcatcga
     3001 ttctgcatgt gttcaatgta cgcagcagcc aatcgatctt caaagtcaat ttagcaacca
     3061 ataaagcttt ggtagaaatg gcgttactgg cattagctat tacaacggct gttgctttgc
     3121 tgccatttac gcaagaacta ttcggcctag tacacatcag tttgaatcac tggttcttgg
     3181 tagggatttt atctatcgta ccaatcgcgg tgaatgaact gatcaagttc catcgattac
     3241 cggaagcaga agaagaggaa tgattaactg tttctaagtt gattttcttt ttactggaac
     3301 tatacaaaaa ggaggtgtga catgaatgtt gtcacacctc ctttttgcta atgggctgaa
     3361 tgtttctttt actgcctctc gataaacggt gttctcctac caacccctcg gtcacaagtc
     3421 acactctctt ttaaatatct tttggcaatt gttcataaaa tgctgctttt gttgcggaaa
     3481 tgtatgggct gagccataga taaccaatac cagcagttgc taatgctaag atatgccagc
     3541 cgataaatga aagatctaac cagaaaagtt gtcctttata accatccatc aattttcgac
     3601 ttgcagtgat cgtatccagt actttaggtg tttgtcctgt ttcttggacg atatcgtagt
     3661 agataaaata gctttgagaa taagaatagc ttttaatgat tccaggaata actaatagca
     3721 aaccccataa tgttgtaaag atcgagatca gcacagctaa gccgatcact ccgccgataa
     3781 acaaaccttt gaatccacga aatgcatctt tgaatggttc gatctgatct ttttgtccac
     3841 gtaaaatatc aagataagtc caagaaatac cgctcatgaa caaagcgcta atgatgctgg
     3901 aaataattcc gccaccgcct cctgaactac cgcctcctga agttgtcgca tctgagacgc
     3961 tcgctgatcc atcatatatg gtggtggcaa ataagataat aggaatcata ataagaagaa
     4021 tagcagcaat accaatcaat gtagggatta gatttagtaa aacagcttgt ccccaacgtc
     4081 cttttagact ctcttttgct tggcgtttta attctgcaga agattgcatg atttttccct
     4141 ccttttcttt aataacacca atataccatg tatcaaaaaa tatttctgtc ataatgtttt
     4201 tctctttttt gttatt
//
LOCUS       DACEUF010000129         4109 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.135, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000129 DACEUF010000000
VERSION     DACEUF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.135"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            154..309
                     /locus_tag="IXG20_002525"
                     /pseudo
     CDS             154..309
                     /locus_tag="IXG20_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            328..1605
                     /locus_tag="IXG20_002526"
     CDS             328..1605
                     /locus_tag="IXG20_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1610686.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1615..2271
                     /locus_tag="IXG20_002527"
     CDS             1615..2271
                     /locus_tag="IXG20_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1610687.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2271..3647
                     /locus_tag="IXG20_002528"
     CDS             2271..3647
                     /locus_tag="IXG20_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1610688.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3777..>4109
                     /locus_tag="IXG20_002529"
     CDS             3777..>4109
                     /locus_tag="IXG20_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1610689.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1628 a          452 c          725 g         1304 t
ORIGIN      
        1 aggcttacca gcgagataga gatcgacgat ttgttgtttg aattcttttg aataggttct
       61 tcgttgacgt cttggcatga aaaatcctcc agtgtgtttt ttattattct acacacctta
      121 ttttttctgt ctagtctagt gtagccgatt caggataagg ataagtgtat taattgtgga
      181 aaatgtaaaa aagtttgcca aatgaataaa gattcagttg agaattgcaa tcatctggag
      241 tgtatcagat gtggacgatg taaaaatgct tgtccagtgg atgcaattag ttatggagta
      301 agaaaataga aaggagtagt taggaaagtg ataaaaaatg ctatagcata tattacaaga
      361 aagaaaaata gaactttaat aatttttatc atattaacta tagttctttc ttgcttgtat
      421 tcttgtttaa caataatgaa atcaagcaat gaaatagaaa aggctttata tgaaagttct
      481 aattcttcaa tatcaataac aaaaaaagat ggtaaatatt ttaatgttaa tcaatttaaa
      541 gatattgaaa aattaaaaga aattgaagaa aaaataattc aatatgatgg attagcaaaa
      601 ctaaaagatg ctaaagtagt tagcggagaa caaagaataa atagagaaga tttatctgac
      661 gaatttaaga atgttgtttc actcgaagct acaaataata ctaaaagaaa tattttattt
      721 agtagtggag tatttacaat taaagaaggt aaaaatatag aagaaaatga taagaattca
      781 attattgttc atgaagaatt tgctaaacaa aacaatctaa aattaggtga tgaagttgat
      841 cttgaattac tagatattga aaaaagtgga aaaataaaaa gtcataaatt taaaattata
      901 gggatctttt ctggtaaaaa acaggaaaca tatacaggat tatcttctga ttttagtgaa
      961 aatatggtgt ttgtagatta ttcaacaagc caagagatat taaacaattc agaaaataat
     1021 gaaattgcaa ataaaatttt aatgtattct ggtagtgcag aatctacaga cttagcctta
     1081 aacaaattga aagagcttaa aattgatgag tcaaagtatt ttgttgaaaa agattctaat
     1141 gcgtttgaag agtctttaga gtcagtgagt ggaataaaat atataataaa agtaatgact
     1201 tattctatta tgttgggtgg gatggttgtt ctttcattaa tcttgattct atggttaagg
     1261 gaaagaattt atgaaatagg tatattctta tctattggaa catctaagat acaaattata
     1321 atgcaattta tattcgagtt aatattcata tcgataccaa gtataatatc ttccttattt
     1381 ttaggaaatg tattactaaa agtaattgta gatggattca ttaactcaga ggattcaatg
     1441 atttccggtg gaagtttaat aaataatagt agttttatgt caaatataac aacacttgga
     1501 caaagttatt taatattaat aagtattatt gttttatcag ttgtatttgc ttcttcatta
     1561 atgttaatca agaagcctaa agaaatctta tcaaaaataa gttaggagaa aataatggat
     1621 atattagaaa taaagaatgt agcatatagt tatgcaaatt ctaaagaaaa agttttgtca
     1681 ggagtaaatc aaaaatttga acttgggaag ttttatgcga tagtaggaaa gtcaggaaca
     1741 ggaaaatcta cacttctttc cttacttgct ggacttgata aacctcaaac aggaaaaata
     1801 ttgtttaaga atgaagatat acaaaataaa ggatatagta atcatagaaa aaataatata
     1861 tctttagtat ttcaaaacta taatttaata gattatttat caccaattga aaatattaga
     1921 ttagtaaata aatcagcaga tgaaagtatc ttgtttgaac taggtttaga taaaaaacaa
     1981 ataaaaagaa atgttatgaa attatctggt ggacagcaac aaagagtagc tattgctaga
     2041 gcattggtat cagatgctcc aataatacta gctgatgagc ctactggtaa tctagacagt
     2101 gttactgctg gagaaataat taatatatta aagacattag ctaaagatag aaataaatgt
     2161 gtaatagttg taacacatag taaggaagta gcagattctg cggatatcat tttagaacta
     2221 agtggtaaaa agttgaaaaa agtaaataaa atgaatttgg aggttgaata atgataaaaa
     2281 atgcatttgc ttatgtaact agaaaaagtt taaaatcatt aattatcata ttagttattt
     2341 tatctatgtc aactttaagt ctcattagtt tatctattaa agatgctacg gacagagctt
     2401 caaaagaaac atttgctaat ataacaaata gtttttctat ggagataaat agacaagtaa
     2461 atccaggaac acctagaggt gggggaaatg taaaaggtga agatattaaa aagatatctc
     2521 aaacagatag tatagactcc tatgtaaaaa gaataaacag tgttgcagat ttagttgatt
     2581 atgatattat tgaaactcaa gagactcttg cgaatcaatc acctgaaaga gcaaagaatt
     2641 ttaaaagaac agttatgtta actggggtta acgactcaac aaaagaaaca aaatttgtat
     2701 cagaagcata taaattagta gaaggaaagc atttagaaaa tgaagataaa aataaaatct
     2761 taatgcataa agatttagct gaaaaaaata accttaaagt tggagataag ataaaaataa
     2821 aatctaattt atttgatgct gataatgaga aaggtgcaga tgaaacagta gaagtagaaa
     2881 ttaaaggtct attcgatggg cataatagcg gtggagtaag tgcagcacaa gaactatacg
     2941 aaaatacatt aatcactgat attcatacag ctgctaaagt ttatggtaat accgaagata
     3001 cagctgtata ccaagatgca acattctttg taaaaggtga taagaatctt gatagtgtaa
     3061 taaaagatct tgggaaatta gatataaatt ggagagaata taatttgatt aaaagttcat
     3121 caaattaccc tgcattacaa caatctatat caggtattta ctcaatttca aataaattat
     3181 tttttggctc attaatattt gcaggagttg tagtttcatt attattattc ttatggatga
     3241 atgctagaaa gaaagaaata gcagtattac tatcattagg tatatcaaaa ttagaaattt
     3301 ttggtcaatt cctaattgag atggtattta tatcaatccc cgcattcgtt ggttcctatt
     3361 tcttagctca atatacagct gacaagcttg ggaataatat attgaataag gttactggtg
     3421 atatagctaa acaaatagct aaacaatctg catctagcca attaggtggt ggggcagaag
     3481 ctgaaggatt taacaagaca ttatcaagtt tagatatcaa tgtattacct aaattcatga
     3541 tttatgtagt tatatttatg agtttagtac ttcttgtatc attgattatt tcttcattca
     3601 atatattaag aaggaatcca aaagaattat taattgacaa taattaaaat tttcggtgct
     3661 ttttagcacc aaaaatgaga gtgtaatata aaccgtgtaa gtaaccatca atcctaggcg
     3721 ggtggttgct tcacggtttt tatttttcta aaataagatc aagaggtgat tacacgatgg
     3781 ctagaaagaa aagaaatcct gatgctgaga agttagctga atccattttg aatacttatc
     3841 aacccgaatc tgtagaggat atgcaagatg ttttaaaaga tgtgtttggt cccttatttg
     3901 aaaaaatgct tcaaggagaa ttgaataacc atttagggta tgacgcccat tctaaagagc
     3961 caaagaaaca tgacaatcga cgaaacggtt atggcaataa gacgcttaaa acgagctttg
     4021 gtaaagtaaa cattgatgtt cccagagacc gggaggcttc ttttgaacct gagttgattc
     4081 ctaatagaaa acgagatgtc tccgacatc
//
LOCUS       DACEUF010000130         3925 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.136, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000130 DACEUF010000000
VERSION     DACEUF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.136"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(573..1130)
                     /locus_tag="IXG20_002530"
     CDS             complement(573..1130)
                     /locus_tag="IXG20_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610690.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IXG20_002531"
     CDS             complement(1309..1920)
                     /locus_tag="IXG20_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610691.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IXG20_002532"
     CDS             complement(2033..2290)
                     /locus_tag="IXG20_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610692.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="IXG20_002533"
     CDS             complement(2887..3165)
                     /locus_tag="IXG20_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610693.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="IXG20_002534"
     CDS             complement(3199..3450)
                     /locus_tag="IXG20_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610694.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DACEUF010000131         3627 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.137, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000131 DACEUF010000000
VERSION     DACEUF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.137"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(148..969)
                     /locus_tag="IXG20_002535"
     CDS             complement(148..969)
                     /locus_tag="IXG20_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1610695.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(1066..1344)
                     /locus_tag="IXG20_002536"
     CDS             complement(1066..1344)
                     /locus_tag="IXG20_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610696.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            1612..3621
                     /gene="metG"
                     /locus_tag="IXG20_002537"
     CDS             1612..3621
                     /gene="metG"
                     /locus_tag="IXG20_002537"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ1610697.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
BASE COUNT         1203 a          668 c          712 g         1044 t
ORIGIN      
        1 ttcgtacgag ctttttctgc tattgttaat tttttattca gaaagtttac gaatacatca
       61 aaattctgaa acaaaaaacg taactcacaa agagttgttt ttgcgttttt tcttgaatga
      121 ttaggtctga atcttttgct cacaacctta ttcatggtgc cccggcttga gctgatagat
      181 ttttgaagga cggccgattc ctacaggacg ttcaccgatc tcatcgaaat attggagcat
      241 agctttctta aaattggaat gatcgatcgt tttataatcg atccctaaga atttagcaaa
      301 tacttttcga gcttccgtaa tcgtgaaatc tttacctaaa acttgtagaa cttgcggctc
      361 gtgttccatt ttattgacta cacggttgaa tgctttcaag atgatttgac tatggtcaaa
      421 agccaaagta tctttgccag aagactctcc agtgactaaa tcaagcgaga tagccacttc
      481 tccgctagat aaatggatct tgttactatg acgttctagt gtgaaccagc gtacttcctt
      541 ggcatcatct ccggcgatta gcggttcttc tccaataaag gctaagtagc tgactgtgac
      601 gacccagcct cgcggatctc gattaggcgt actgaatgta tgcagttgct caatattttc
      661 ttttgaaata accacaccag tttcttcctt tgtttctctt aagacgcttt ctccagttga
      721 ctcatccttt tggacaaagc caccaggcaa tgcccatgaa tttcgataag gatgcccttt
      781 tctttggatc aacaaaactt tcagctgatc ttcttctttg ttatagcaca tcagtacgat
      841 atcgacagtc aaagagggct tgtcatattc tggacgttcc tgtttttgat accagttaag
      901 aaattcttct tcacttgctt tatgttcata atataatttt tcttcttctt tagaagcaaa
      961 acggttcatc tgaccacctc cattgacagt aaaagtactt tttaccttat tgtagcagac
     1021 tctgaaaaaa aaccaaggat ttacttgaaa aatctaggtt atcatttaaa tatgaaaaat
     1081 aaatggaagt atgagattga ataaaaagaa aagcagataa aacggcaata ctttataccg
     1141 ctcttcacga atggcaaaga agccacaaat aacgatatat acgtcaatga taaaacgcaa
     1201 aatccaagct gctgtcataa aactgttcaa atgcccatgg aggttaaaat aaccatacat
     1261 acgaatataa aaatagctga atattaaatg tgctaatgaa agaaaagcaa tactaagtgt
     1321 aaaaaaaaga tgtttcttct gcatgattgt tccagtccct tactaagata gatagtcttt
     1381 attataccac gtgtgagggg agaaataaaa agccgttttt tagtagatat gaaacaaaac
     1441 aaaaacccgc atttattcaa cctattcatc cttttaaagg atagattgaa cgtagataga
     1501 agtcgtttat ccagtctttt tcattttgaa aaaaagctaa cagcatgctt tttttatggt
     1561 atactagcaa ggaaacgaat tcgcatcatg cgataaagga ggaagagccc cgtggcagaa
     1621 aaggaaactt tttatatcac tacgccaatc tattatccaa gcggcaaact tcatatcggc
     1681 aactcgtata ctacgattgc atgtgacgca attgctcgtt ataaacggtt gatgggcttt
     1741 gatgtgtttt atctcactgg agtggatgaa catggtcaaa aaatcgaaac aaaagcagaa
     1801 gaactaggtg taaaaccgca agaatatgta gataaaatgg cggctgatgt caagaaatta
     1861 tggaaaacgc ttgatatcag ttatgataaa ttcattcgca cgacagatga ttaccataaa
     1921 gcagctgttc aaaaaatatt tgatcgttta ttagaacagg gagatattta cttaggtgaa
     1981 tatgaaggct ggtactctgt ttctgacgaa gaattcttta cagaaacaca acttgcagaa
     2041 gtgtacaaag atgacgaagg taacgtgatc ggtggtaaag cgccaagtgg acacgaagta
     2101 gaattggtaa aagaagaatc atacttcttc cgcatgagca aatacgcaga ccgtctattg
     2161 gaatactatg aagaacatcc cgaattcatc cagccagaat ctcgtaaaaa cgaaatgatc
     2221 aacaacttta tcaaaccagg attagaggat cttgcagttt cacgaacaac ttttacttgg
     2281 ggagtaccag tcaagagtga tccgaaacat gtggtgtatg tatggatcga tgcattatcc
     2341 aattatatta ctgcactagg atatggctcc gaagatgaaa gtctattcga aaaatactgg
     2401 cctgcagatg ttcagatggt cggtaaagaa atcgttcgct ttcatacgat ctattggcca
     2461 atcatgctga tggcccttga tttgcctcta cctaaaaaag tatttgggca tggctggcta
     2521 ttgatgaaag acggaaaaat gtctaaatca aaaggaaatg tcgtttatcc tgaaatgctt
     2581 gtggaacgtt atggtttaga tgcactccgt tattatttgc ttcgtgctgt tccgtttggg
     2641 tcagatggtg tctttacgcc agaagatttt gtttcccgtg tcaattatga tctggcaaat
     2701 gatctaggga acttattgaa tcgaacgatt gctatgatca acaaatattg tgatggactc
     2761 gttcctgact atgcctcact tgtcactcca tttgatagtg aactgtctac gacggctgct
     2821 aacgttgtcg gacgttacca tgatgcaatg gaaaaaatgg aattcaatac agcattggct
     2881 gaaatctggg tgctgatttc tcgtgcaaac aaatacattg atgaaacaga accatgggta
     2941 ttagcaaaag acgaagaaaa gaaagctgaa ttgaatagtg tcatgatcca tttagctgaa
     3001 tctttgcgta tcgcagcgat attacttcag ccaatcatga cagaaacgcc agagaagatc
     3061 ttcaatcaac taggattgga ctctgaaaca atgaatcttg aaggattaca ctttggcgaa
     3121 tttccttcag gaacaaaagt agttgcaaaa ggtacaccaa tcttcccacg tttagatatg
     3181 gaagaagaag ttgcttttat tcaagaaaaa atgtctgaag gtactcaaac aaacgaagat
     3241 acagtcaaat gggatccaga agaaacagaa cttgtttcaa cgaaagaaaa acaaatcaaa
     3301 tttgatgtat ttgaaaaagt agaactaaaa gtagcagaag tcatcaactg tcaaaaagta
     3361 gaaggggcag ataagttgct gcaattccga ctagatgctg gagacagcca agaccgtcaa
     3421 atcttgtcag gaatcgctga attttatcct gatccaagcg aattgatcgg gaaaaaagtc
     3481 gtcattgtag caaatctgaa accacgaaag atgcgtggac aaatcagtca aggaatgatc
     3541 ctttctgcag aagcaccaga tggctcttta caagtaatcg aagcaccaaa atccatgcca
     3601 aatggctcag aaatcgcata gaaattc
//
LOCUS       DACEUF010000132         3390 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.138, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000132 DACEUF010000000
VERSION     DACEUF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.138"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            647..964
                     /locus_tag="IXG20_002538"
     CDS             647..964
                     /locus_tag="IXG20_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1610698.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="IXG20_002539"
     CDS             complement(1055..1420)
                     /locus_tag="IXG20_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610699.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="IXG20_002540"
     CDS             1628..2110
                     /locus_tag="IXG20_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610700.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /gene="pstS"
                     /locus_tag="IXG20_002541"
     CDS             2307..3197
                     /gene="pstS"
                     /locus_tag="IXG20_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ1610701.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1172 a          519 c          619 g         1080 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac
//
LOCUS       DACEUF010000133         3298 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.139, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000133 DACEUF010000000
VERSION     DACEUF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.139"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            138..1106
                     /locus_tag="IXG20_002542"
     CDS             138..1106
                     /locus_tag="IXG20_002542"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ1610702.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(1423..1947)
                     /locus_tag="IXG20_002543"
     CDS             complement(1423..1947)
                     /locus_tag="IXG20_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ1610703.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            2234..>3298
                     /gene="scm"
                     /locus_tag="IXG20_002544"
     CDS             2234..>3298
                     /gene="scm"
                     /locus_tag="IXG20_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1610704.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         1052 a          667 c          611 g          968 t
ORIGIN      
        1 aaaaatgaac gcccaccctc ttcttttttc tctttccttc tttcgttttt gctttatgta
       61 tcaaccgttg tttcttcgaa aactattttg ttctctcctt cttcctgata gaatggatgg
      121 gaaaggagct tgatgccatg acaacgaaag acaaggtact tcaattattg aaaaagacac
      181 cagattttct ttccggagaa aagttggcac agcaactgga agtttcccgg acaagtattt
      241 ggaaagccat caaagaattg gaaaaggcag gctatcgttt tgagcacggg cctacagggt
      301 atcgttacct gccctctgac gtactagatg caaaagaaat ccagcaagag ctacggtcgt
      361 ttattcctga tttgaaagta caaacgatgt tgacttctga atctacgatg aaagatgcca
      421 aactggcagc agctcaagga gattctgttt cgaagttatt tgttgctgat acacaagaac
      481 aagctcgtgg acgttttgga cggccttttt tcgctgaacc tggacgagga atctacatga
      541 gtctcttgct ccatccagac aaacggtttg atgaattgcc tcaatatacg atcatcgcag
      601 ccagtgcctg tgtgaaagcc attgaatctg taaccggtcg ttctgcttcg atcaaatggg
      661 tcaatgatat ctacatggat ggaaaaaaag tctgtgggat tttatctgaa gcagtcagtg
      721 atatggaaag cggcaggatc agtcatatca tcattgggat cgggcttaat ttttctgttc
      781 ctcaaaatca ttttccagct gatctccaag aaaaagccat ctctgtcttt cccgacggac
      841 aagctagtgt cacaaggaat cagctaatca gcaaaatctg gcaagaattg ttcacttatc
      901 tttctcaccc agaaaaagcg ttggacttct ataaagaaaa atcattcgta ttgggaaaaa
      961 ccgtgtcgtt caatcaaaaa ggaacgactt atgtcggtct tgcaaaagaa atcggtgatc
     1021 atggcgaact gatcgtccaa acaaatgaac agaagatgag tttgtcttct ggcgaaatca
     1081 gtctcacatc tatccgtacg caataattat cagatttcac aaaaggacac ctgatctcat
     1141 acagtgtcct tttttctgaa ttctcttcaa aagactctcc ttattctatc agctgacagt
     1201 ttctctatcc tctagaaaaa ggtattctaa aaatatctat aaagcatggt acactttctt
     1261 taaatgaatc aatgcaaaaa acaagagagt catcagttta cacgatcaaa agagatagca
     1321 ggccgcttcc tttttcaatc ttatagatag accatgggta acgataaaaa caaaaaagaa
     1381 cttgcgactt attttgccgt aagttctttt ttagctagtg ccttaatctc ttaatcccaa
     1441 caaacgtctc gcatagatca cctgaccaga gtgcatagca gcatcatcaa taatagaaac
     1501 gagacgcatt cctcgtgtga ctggtggtgt ccagttctcg tcaattactt catctagctc
     1561 ggaaggttcc agccttctta agtaagaaat agcaaaatca gcagctcctc gcaagtatcc
     1621 tagcagatct tctatttgat tagcttggat ctgctttgct tgttccagac tatgcttcca
     1681 atcttgttcg ttatctgcta tcgtcatcgg aaaccggctt tcccagtctt gactatgcca
     1741 aattggagaa gttcctgcta aatcagcaat ctggaaatct aattctcgag cggtatgcca
     1801 agccagccaa gtcattgatt tgatctgcgg ggctctatct tccactggaa aacgattggc
     1861 ctcctcaata gagacgcctt caaacaaacg ttcaaaccga tcgtatgccc gatccacact
     1921 ctcgcatagc atatctaaat aatccattta ttttcctcct ttttatttat cctacttact
     1981 ttttcctaat tctaggatac ctacttatat gttactacac acccttattt tttacacttt
     2041 aacagtcacc atcatcaata gtttggagat tgaaagaaaa agatagtgtc gaactatcaa
     2101 ttgaacaaga agcaacaata tgaacgtttc attaatattc tttaaataaa caatgatttt
     2161 tcaaacgaac agtttaagac taggagaaaa agagtataat aggaaaatgt ttgaaaggaa
     2221 aggaagggga aaaatgaaaa aaagggcaag tttactagtc ctagttgctt tgttgttcaa
     2281 tatcttttcg tatggtgtgg ccgtttcagc ggaaacatac attcctaaag accctgggaa
     2341 tatttataat gatctaactg gtaactatgg cttgttagga attgctagtc aattccacgt
     2401 atttgctaaa aataagacaa ctgtgaatgc acatacagac ggaaatatag caacacatga
     2461 acttgatgca aaaaataatt ttggtacaga gcttcatgaa ggtttattga accaagaaat
     2521 aaattatata caaaaaactg attctttcgc taattcttca ggcataccta caggtagcac
     2581 aagaatgaat aaatttgtcg ttggcagtaa cgtggatgta gcacttcaag atggtcatcc
     2641 tacagtcaat ggatccaggc ttgatcactt aactccagat gatttttata aagatcgtgc
     2701 tgggcatgaa tatattgatt tcgagcagga atttgctaaa ttgaatgtag cagcaaatga
     2761 tcttgcaacg atcactccag ctaaaacata tactgctgca gattttccag acatgaacaa
     2821 tcggacaatc gatttaactg atttatcaga taccgggttt ttattggtaa atatcgatgc
     2881 agaagtgtta acgatgaata cgccacttca aatcattaat ccaaatgatc aagtagttgt
     2941 atttaatgtc atcaacagtt cttctgcatt gaacgtacag tcaccaatca aatacaatga
     3001 tcgttcaaat catgaaacag aagatttttc ggatgcgaat atctcttgga attttggaaa
     3061 tgagatgact gatttgacaa tcagtgctcc ttttcaagga acaatccttg cgcctaatgc
     3121 tactatccgc attactcaaa accaagacgg aacgattata ggaacaaatg tgattttaga
     3181 tgcagctacc aatcgttggg atcctaacga aatatttatc agcgacaatg gtacagatac
     3241 gacagacaca agcgacagta cagatacatc taccacaagt gatagctcag attcatca
//
LOCUS       DACEUF010000134         3282 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.140, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000134 DACEUF010000000
VERSION     DACEUF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.140"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            500..787
                     /locus_tag="IXG20_002545"
     CDS             500..787
                     /locus_tag="IXG20_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610705.1"
                     /translation="MEIKEIKVRGINKKYVQEIDHRCVELTEQTGQKWSRNDYLKLLI
                     ENDFERPLMDYKKDQFDRLLEKFTDVQLHNTKVLEAYTNEVKNLIEILIAH"
     gene            805..1227
                     /locus_tag="IXG20_002546"
     CDS             805..1227
                     /locus_tag="IXG20_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610706.1"
                     /translation="MVKAIEKHITFYEAHDEVLKHIMETQPTVKNYSEAIRFLCMNYD
                     PEIEKNNNMTNAKLNAMGKEISILIEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKSINESVHARVDPFDPTKFLKR"
     gene            1247..2839
                     /locus_tag="IXG20_002547"
     CDS             1247..2839
                     /locus_tag="IXG20_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610707.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQNSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQIEQERQ"
     gene            3095..>3282
                     /locus_tag="IXG20_002548"
     CDS             3095..>3282
                     /locus_tag="IXG20_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610708.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1317 a          502 c          629 g          834 t
ORIGIN      
        1 atttgacaaa tcatatgaaa aaagtatata aaaaatgttt atggatcagc tatatcaccc
       61 taggtatagc tgttaaagag atgggagaaa acattaacca cgattctaac acaaacatca
      121 agcacataaa acatttaaca ctcctccttt ctggctgaag atttcaacca aacaggaaga
      181 cagcgtatca attatagcat acactttttc ttaaattcat ttttcatatg acaaaatatt
      241 tccttctaat cgacaaagtt agaaggattt tttattgtct aaatacacaa ataaccttaa
      301 tttttaaaga gcataaggaa acgtgggaac actttttaaa aaaagtgttc caataggctg
      361 tgcctattac gtttctctaa aacaaaagtg ggtacaaaat attcgattta tggatagtgt
      421 atggatagta aaacagctat acatggatag catatggata gactccatag actatccata
      481 cttttttcgg agggtgaaaa tggaaattaa agagattaaa gtaagaggta ttaataaaaa
      541 atatgttcaa gaaattgatc atagatgcgt agaattaaca gaacaaactg gtcaaaaatg
      601 gagtcgaaat gattatctaa aactattgat tgaaaacgac tttgaacgtc ctttaatgga
      661 ctacaagaaa gatcaatttg atcgtttatt ggaaaaattt acagatgttc agctgcataa
      721 tacaaaagtt ttggaagcat acacgaacga ggtcaagaac ttaattgaaa tattaatcgc
      781 acattaggaa ggagataatg ttctatggtt aaagcgatag aaaagcatat tacattttat
      841 gaagcgcacg atgaagtttt aaaacatatt atggaaactc agcctacggt aaaaaactat
      901 tctgaagcca ttcgattttt atgtatgaat tacgatccag aaattgaaaa aaataataat
      961 atgacgaacg caaagttgaa cgcaatggga aaggaaattt ctattttaat agaaatggtt
     1021 tcagagtttg cggatcttca tttgaaagat acaggaatat taattggtac aaattcaact
     1081 gtctatcaag aagcaaaaga gcgtgtggaa gggaaaataa aagcgaatca aacgaagaag
     1141 tattctccta aaatcaagaa atcaataaat gaatctgttc atgccagagt agacccgttt
     1201 gatccaacaa agtttttaaa aagataaaaa gtagaagggg aatatgatga ataaatcgcc
     1261 tgcagtcatt ctcacttgtc aatttacctt gcccaataaa aaatcttttt caacatacat
     1321 tgattatatg acaagagaaa aagcgctgga agaaatggaa cgacgtacgc ctgaagaaga
     1381 acaggaactg caacgaatca agaatgcttt agaggatttt tatattcctg aaggagaaac
     1441 tttttcggag agtaagacgg ataaagaatt cagcgaaacg tcgaatgaag ctcaatcttt
     1501 gatgggaaat ggaatcaact ttgacaagtt agaggaacaa gattttacca aatatctttc
     1561 ttatatgacc cgtcactatg cgttagaaca aaagaaagaa ctgacgcaaa gtgaacaaaa
     1621 agaataccaa cgcttgaacc aagcaataaa aaaatatcaa acagagccac acaaaaaagc
     1681 ttcaggaaac cttcctggag ttttttctat ggcttcagac gaagtgaaag gaagtgatct
     1741 gaaggagatt aagcaaatct tccaaagagg acaacaaaaa ggctcaatca tttatcaaga
     1801 tgttgtctct catgataatt cgtatcttga aaaaatagga ttatacgatc ccaaaacaga
     1861 tactttagat gaagaaggat taaaagcagc tggaagaaaa atgatggaga cattattcga
     1921 gaaagagcaa ctgaacgaaa caggctactg gatggcaacg attcaccgta ataccaaaca
     1981 tatccatatt cattttgcag ttgttgaaaa agaaaatacg cgtaaacctt acacggaggt
     2041 tgaaaatggt gtgagttata tcgtaccgaa aggtaagaga agacaagcaa cattagacgc
     2101 aatgaagacc aattatgtac atgagttaga acgtttttct tatgaaaagg atcgaatgat
     2161 tggtatggag aaacgaaatt tattgacgcg gaaaagtgat ttgcgtaaca ccttaacaaa
     2221 agtggtaaaa gagccagcgc gttacgatcg acacgcaatg aacttattaa atgaagtata
     2281 taaaacccta cctgacaaac gttcggaatg gcattatggg gatgaaaaac gaaccaaact
     2341 ttcaccatac acaagagaga aactcgatga gttaaccaaa tatgttttaa caaaagaacc
     2401 tgaatatcaa gaatatatac agatttcaaa agcattgaaa gaagaagcaa aaagtcttta
     2461 tggggaaagc aaaagagaat caaaggatgc agaaaaaaat gctctctttg atttgaaaaa
     2521 gcgcacagga aatgccattc taacagaact gaagaaacag aatagtcaaa taaaaccttt
     2581 aatagaaagt atggatcaga tgcaatctat ttacaatgaa cgatcctttc gaacgcttaa
     2641 acagttacca acgaatgaat acaaatatag ttacgaagaa ttcatgaagc gcaggaaaca
     2701 attccaacaa agagttgtca cgcaacggag tttgcgtcaa ttaaatcgaa cgattaatgc
     2761 ccatgaagaa aaataccacg caagtaaagc ctacgaagag caacaacgaa gaatccaaat
     2821 agaacaagaa agacagtaaa gacagtaaag ttagcgcctt attatactac ttgggaaatg
     2881 aatgaagagg ggatcaataa cttcccgtgt aaaagcattg aagagtctat ttcactcaca
     2941 gaatgttagt tgttaagtga ataaaagaag taaaagagtc ctgaacgtaa ttgtccagga
     3001 ctcttttttt caatgaattg ttttccgcca attcaatgag tggtgcgatt tgcgactaca
     3061 ttatatcaaa agaaagagaa gggaggaaga agaaatggct tattcaacga ttggagttgt
     3121 tgttgaaaaa agcagagaca atctcgtttt tgtgacagaa attcaaacag gcagagcttt
     3181 tgtcgtcaca gacaaagcgg caaaagcgta tcaaaatggc gatattttaa cgttaaatct
     3241 gactacgaag acgttcgtcg atgcggcaga agattatcca tt
//
LOCUS       DACEUF010000135         3261 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.141, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000135 DACEUF010000000
VERSION     DACEUF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.141"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(368..1486)
                     /locus_tag="IXG20_002549"
     CDS             complement(368..1486)
                     /locus_tag="IXG20_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610709.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..>3261
                     /locus_tag="IXG20_002550"
     CDS             1957..>3261
                     /locus_tag="IXG20_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ1610710.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
BASE COUNT         1019 a          678 c          588 g          976 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt t
//
LOCUS       DACEUF010000136         3234 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.142, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000136 DACEUF010000000
VERSION     DACEUF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.142"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            183..1137
                     /locus_tag="IXG20_002551"
                     /pseudo
     CDS             183..1137
                     /locus_tag="IXG20_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 105 protein"
     gene            1154..2178
                     /locus_tag="IXG20_002552"
                     /pseudo
     CDS             1154..2178
                     /locus_tag="IXG20_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            2184..2663
                     /locus_tag="IXG20_002553"
     CDS             2184..2663
                     /locus_tag="IXG20_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ1610711.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            2679..>3234
                     /locus_tag="IXG20_002554"
     CDS             2679..>3234
                     /locus_tag="IXG20_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ1610712.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
BASE COUNT          979 a          540 c          751 g          964 t
ORIGIN      
        1 cttggaaaac gttgtttctc caagttcttt ttttaatttc caagtaactg aaagttactt
       61 ttgttattta acatttaggt caattttaga cagtttttgt tcaaaaagcg atatttatag
      121 tggacggtat tactgatata ttaaggatat aaaatgaaag cgtttaaaaa ggtggcgtaa
      181 agatgattat taaagaagag atttttgtac ctaaagaatt agtgaaagaa aaaattgacc
      241 tgctcgttga aaacctgaca acgatcaaag acgataatgg tgaatttttg ttgggctttg
      301 atggactgaa agtagatgac aagagctgga cagtgtggaa ttggccacaa ggtgtcggtc
      361 tatatggtat ttataaaaat taccagatga caaaaaatcc tcgcgcttat caagtggtca
      421 atgaatggtt tgaggatcgt atggaagaag gcgctccgcc caaaaacgtc aatacaatgg
      481 caccgctatt gactatggct tacttgtatg aagacacgaa agattcacgc tatttgcctt
      541 atttagaaca atgggcagaa tgggtcatga atgacatgcc tcggacaaat gaagacgggt
      601 tacagcatgc cacatatgga ccggaaaata agaatcagtt gtgggacgat acattgatga
      661 tgacagtgct tcctttggca aaaatcggga aactgctggt tgacgattgc tattccagag
      721 atcattgaaa tcttagaatt atcgaaagaa gatgcattgc gcaaattatt gatcgaaaca
      781 cttgaagcac aagtgcgtgc attaaaaact tatcagtctg aaagcggttt atggcatacg
      841 ttgttggatg atccttcttc ttacgtagaa tcttctgcaa ctgcaggatt tgcttacggt
      901 attttgaaag cggtccataa acgttattta ccacaagaat ataaagaagt cgcttatcgt
      961 gctatccaag gattgcttga acaaatcgat gaaaaaggag aagttcaaaa cgtttcaatc
     1021 gggacaggaa tgggggacag tttagatttt tatcggaata tcggaatcac agcaatgcct
     1081 tatggacagt cgttaacggt gctttgtttg acagaattgc tcgtctctta ttgttaaaag
     1141 aaaggaaacc catatgaaaa aaacatcact gctgtttgca gcaagttcgc ttagcctagg
     1201 tctgctaggt ctaagcggct gcggaaataa ttctagtgtg gcaactgtcg cagatgcaga
     1261 cgtaaatgag atagttacct tgcgctttgc ttatgcaagc aacagccagt aattgatgca
     1321 atgaatgaat ttgggcgttt agtaaaagaa aaaacagatg gttccgtaca gatccagtat
     1381 ttcccagatg gacaactggg tggagaacgt gaactgatcg aattgacaca gtcaggagca
     1441 gtagatttca caaaagtaag tgcctcagcg ttggaaagtt tctctaaaga ttattcgatt
     1501 ttttccgttc cttatctatt caatgatgaa gaacatttct ttaaggtgat ggataatgaa
     1561 gaaataatgg agccaatcta ccaatcgacc aaaaatttag gatttaccgg attgacctat
     1621 tatgattctg gtcagctcag cttttatatg gtagacggtc cgatccatac gccggaagat
     1681 ttgaagggaa agaagatccg tgtgatgcaa agcgaaaccg ctatcaaaat ggtagagctt
     1741 ttaggcggtt cgcccgtacc tatgggaagt gatgaagtgt acacgtctct tcaatccaat
     1801 ttgatcaacg gatcagaaaa caatgaattt gtcttgtata cagctggaca tggcggggta
     1861 gctaaatact attcctatga tgagcataca cgtgtgccag atatcatcat catgaatgat
     1921 gccatcaaag aacagctaac tgatgaacag gaaaaagcca ttgaagaagc agcgaaagaa
     1981 tcaacggttt ttgaaattga agcttttgca aaagcaattg aagaagaaaa agctttagca
     2041 caagaagaac atggagtcca attcaatgat gtagataata caccgtttcg agaggcagtt
     2101 gccccacttc atgatatatt caaaaacgat cctacctaca aagaccttta tcaaaagatc
     2161 caagagcaag gagcgtagct gtaatgaaaa tgattttaaa caaggtatta gagatcctgg
     2221 gagctgtttt gcttgttggg atggtcgctg ttgttttatg gcaagtattt tccagaacgg
     2281 ttttaggaaa tccaaatacg gtcacggaag agcttgttcg attcggtctt gtctggtttg
     2341 ccatgttgtc ttccgcgtat gttgtgggac aaaaagcacg tttagccgta acggttttaa
     2401 gtgaaaaatt aactggcaaa aaaagtaaaa ttttagaagt tgttgttcaa agtttatttc
     2461 tggtatttgc tgcgatgatc atggtgtatg gcggatggaa tgctgtcaca ttgacaatgg
     2521 ggcagatctc gccttcttta agtattccta tgggatatgt ttatctgtca gtacctgtat
     2581 cgggggtact catcattatc tatagcctga tcaatctaat cgatacatta agcaacaagc
     2641 cagcaattca agaaaaaata taaaaatgga ggaataaaat ggcaatagaa gccggattag
     2701 tgttattttt tgtatttgtt gtgttgcttg tattagggat gccgattgct ataagtattg
     2761 cattctcatc gatgatgact ttattgttag ttataccgtt tgatgtttct gcttttagtt
     2821 cagctcaaaa aatggtggga agtatcaata gtttttcgct gatcgctatt ccgtttttcg
     2881 ttctctcggg cattatcatg aataatggag ggatagctaa gaaactagtg gattttgcga
     2941 tgttgttcgt cgggcgcgtg ccgggggctt tggctcatac gaatgtgtta gggaatgctt
     3001 tatttggttc catctctagc tcggccattg ccgcttctac cgcgattggt ggagtgttga
     3061 tccctcagca agtggaagaa ggatatgacc ggaagtttgc gacagcggtc aatatcgcgt
     3121 cagctccgac aggtatggtc attcctccaa gtactgcttt tatcatgtat tcacttgttg
     3181 ctggtggtgc ttcgatctct gctttgttta tgggaggtta tctcgtaggc gctt
//
LOCUS       DACEUF010000137         2924 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.143, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000137 DACEUF010000000
VERSION     DACEUF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.143"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            441..1400
                     /locus_tag="IXG20_002555"
     CDS             441..1400
                     /locus_tag="IXG20_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1610713.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1387..1992)
                     /locus_tag="IXG20_002556"
     CDS             complement(1387..1992)
                     /locus_tag="IXG20_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610714.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
     gene            complement(2232..>2924)
                     /gene="tnpB"
                     /locus_tag="IXG20_002557"
     CDS             complement(2232..>2924)
                     /gene="tnpB"
                     /locus_tag="IXG20_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1610715.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSNCGHKDGKKSLEIRKWTCPICHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          939 a          501 c          461 g         1023 t
ORIGIN      
        1 tttgcttttc actaattaca aaatatgtga tataggaagt ttagttattg ttctcataat
       61 gccatcctgt ttttagatta tctagtaatt tttttgttat ttaaaattag gagcagtgcc
      121 aattgtttta attttaaagg ctttttttat atcttctaaa ttagaagata tttgtatatc
      181 aatatggaat atattacctt ttttgtattt ttttatgtta tctttagcta aaacatcaaa
      241 tacatgtcct tgaaattgta tcgaggcagc tggatttagt ttaaattgag gaaactttac
      301 cacagctgat tcaaaacact tgtcacattt ttttattttt ttaccttcgc cgattgtaaa
      361 attaagctag acaactaaaa aagtcatttg tgctacactc aaaatagttc cgtcgaaaga
      421 attataagga gtgagtacaa atgacctata cccatcttac accaactgaa ctcgtaatga
      481 tagaagctta tttcaatcaa tcacactctg tttcaaaagt agcccatctg gtacaacgtt
      541 ctcgacaaac catttataat gtctatcgtt ttcttaaatc tggtggaagc gcaataagct
      601 actatgaaca atataagaaa aacaaacgta actgcggaag acgtccgatt gtgttaccag
      661 atgggcaaca agaatacatt caaaaaaagg tggctcaagg gtggactcca gatgtcctta
      721 ttggtcgagc agagtttcct attgcttgtt cggtacgtac actatatcgg atgtttaaag
      781 ataaaacctt tgaccctcac gatttaccaa tgaaagggaa aagaaaacca aacggacata
      841 aagaaaaaag aggaaaacaa gcttttcgcc gttcgattca ggatagaaca actgattata
      901 tacagttcaa tcatgagttt ggtcacctag aaggtgacac cattgtcggt ggaaaacata
      961 aaagtgcggt gatcacgtta gttgagcgat tatctaaagt gatcatcacc ttgaaacccg
     1021 ctggtaggca agctatagat attgagaata gactgaatca atggtttcaa tctgtaccga
     1081 aacacttgtt taaatccatc acatttgatt gtggaaaaga gttctcgaat tggaaaaaga
     1141 ttagtaatac caatgatatt tcgatttatt tcgctgatcc aggaacacct tctcaacgag
     1201 gactaaatga acattcgaat ggcttattac gtaaagatgg cttatataaa gctatggatt
     1261 ttaataaggt atccgaaacc tttatccaat cggtggcatc aaaacgaaat catattccta
     1321 gaaaatcatt acactatcgc acaccattgg aagtattttt gagttacgta ggtaaagatg
     1381 atttgtctag cttaatttga caaacgaaac tttgtaaaaa tattcgattg aatattctat
     1441 ttggcacgac tcacttggca gtatatgtat gtcaatattt ttcatctgtt tcataggtat
     1501 ttcaaaatct aaactattaa aaacatataa ttccctgtaa ttatttacat ctaactctac
     1561 taaatttatc tttggaacaa tagatcctcc acccattcca ccataataaa aaaaatcttg
     1621 ttctctatca aaatagcaca tattaagctc agtttgttta tcatcctcaa taattttata
     1681 aatcaaacct atataatcat gaatgctggc aaaaattttt tttaagtcaa gttctaaatc
     1741 ttcttcgaaa acacatttaa tttctcctac tgataattga tactctatat tacatgactt
     1801 aattagtttt ttagatcccc cctccagata ggcatatgtt tcaataatta tgttcttttt
     1861 attgcttggc aaatttccgt tatttattgc ataaattaaa aatgactgtc tatttagccc
     1921 ataatctaat acaacttctt catactcata tttctttaaa tatttaatat gaaaatatgc
     1981 tgaattaatc aaaactcgag tgttactgtt gttatttatt gttgttctat tgtgtataaa
     2041 attgtatagg ttgtcatatt ttcttggttt agtgaactac tcaccactta gctaaaccta
     2101 acggtttctt acgtttgaag tgggagcttc ttgggaagag agcatacttg atagcgtatt
     2161 actttactaa cagaggtgcc tcttatttac caagctatcc ccgtagttcc tacggttctt
     2221 gtttatttga tctaagccaa tcccatagag tgtagcctta gaccttcggt caggatattg
     2281 atactagcgt tgatatctcg gtcatgatga gtatgacaaa tagggcaagt ccattttcga
     2341 atttcgagag atttcttgcc gtctttatgt ccacaattcg aacagatttg gctagatgga
     2401 aaccacttat ctactttgat gattttccga ccgtaccagt cagccttgta ttgtaattta
     2461 gacacaaaac tagaccaaga aacatccgaa atactttttg ctagtttatg attacgcaac
     2521 atgccttttg tgtttaagtc ttcaatacag atgatatcgt gatttttgat aatttctgta
     2581 ctcaacttat tcagaaaatc agtacgttga ttcattactt tttcatgcaa tcgtgctact
     2641 ttacgttttt gtgtttgata atttctagct tcaaacagat tgataccttt ttgtttagct
     2701 aataaggcac gtcttgacaa cttacgttgt tcacgtttga gtttcttttc cattttggac
     2761 gtaaatttat tattatcaaa tttttgaccg gtagaaagaa ttgcaaagtc tataatacct
     2821 aggtcaatac caacagcaga attagattta ggtagttcgc gaacttcttc tttgcataac
     2881 aaagaaatat aatatttacc gctagaatga cgtgatattg tagc
//
LOCUS       DACEUF010000138         2844 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.144, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000138 DACEUF010000000
VERSION     DACEUF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.144"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(22..2631)
                     /gene="clpB"
                     /locus_tag="IXG20_002558"
     CDS             complement(22..2631)
                     /gene="clpB"
                     /locus_tag="IXG20_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ1610716.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
BASE COUNT          735 a          623 c          478 g         1008 t
ORIGIN      
        1 ttttatagtc tcaatggaag attagacgat ttcttctagc tcttctgttt tgaaatgaag
       61 ttgtccatca agtaaagtaa tcgttacttt tgatttaggc ataacatgtc ctgcaacgat
      121 ttctttagct agtggtgttt ctacttcctt agtgataaat cgtttcaatg gtcttgcacc
      181 atatgctggt tcataagcat tttcagcaat ccacgttttg gcttcatcac tgattgtcag
      241 caggatttct tgatgttcca gacgttgtgc caattgcgcc accattttgt caacgattcc
      301 tttcacgttg tctaggctca atggtgtaaa taaaatcgtg tcgtcgattc ggttcaagaa
      361 ttctggtttg aagtttccac gtaatagcgt gttgacttgt tcagccactg cttctggaat
      421 cgttccatca gcagtcacgc cttccagcag taactgtgaa ccgatattgc tggtcatgat
      481 cagaaccgta tttttgaaat ctactactcg cccttttgaa tcagtcaagc gtccgtcatc
      541 taggacttgt aacaagatat tgaagacatc gggatgcgct ttttcgattt catccaacaa
      601 gacgattgta taaggatttc gtctgacagc ttccgttaat tgaccacctt cttcgtaacc
      661 tacatagcct ggaggagctc cgactagacg agacacagca tgtttttcca tatattcact
      721 catatcgatc cgtaccatat gatcttctga gtcaaacaag ttctcagcta acgctttcgc
      781 taattcggtt tttccgacac cagttggtcc taggaaaagg aaagaaccta gaggacgatt
      841 tgggtcttgc aatccagccc gtgaacgaat taccgcgtca ctgaccgcat caactgcttc
      901 atcttgacca atcactcgtt tatgaagtgt ttcattcaat ttaattagtt tttctcgttc
      961 accttctaca agtttcgtta cggggatacc cgttaatctg ccaacgactt gagcaatctc
     1021 attttcggta acggactctt gaaccatctt aatctcgctg tcttttgctt tagcttctaa
     1081 ttccttcaat tctttttcta attgaggtat cgtaccatga cgcaatacag cagcgcgttc
     1141 taaatcatag ttattttctg catcttctaa ttcgtgtttt gctttatcga tttcagcacg
     1201 ttttgctgaa acagaattga cttcttcttt ttctgtttcc cactgcatct tcatcgcatt
     1261 tgcttcttca cgaagttcag ctaattcttc ttgcaaattt ttcaagcgtt ttttacttgc
     1321 atcatctgat tcttttttca gtgcagcttc ttcgatttcc aactgcatca aacgtctagt
     1381 aacttgatct aattccgttg gcatagagtt catttctacc cgaatcgttg cactggcttc
     1441 atcgatcaaa tcgattgctt tgtccggtaa gaaccgatca gtaatataac ggtcagataa
     1501 agtagctgct gctactaatg cgttgtcatg gatgttaaca ccatggtgga tctcaaaacg
     1561 ttcttttaat ccacgcagaa tactgatcgt atcttccact gttggttctt tgactaatac
     1621 cttttgaaag cgacgttcca acgctttgtc tttttccata tattgacggt actcatctaa
     1681 agttgtggca ccgatcaaat gtaattcacc gcgagcaagc attggtttca acagatttcc
     1741 ggcatccata cttccttctg tttttcctgc ccctacgata ttgtggattt catcgatgaa
     1801 caagaggata cgtccatcac tttttttgac ttctttcaag actgctttta gtctttcttc
     1861 aaattcacca cggaattttg ctccagcgat caatgcaccc atatctaatg aaaaaatcgt
     1921 tttatctttt aaattttcag gcacatcttt gcgtacgata cgttgagcaa gcccttcaac
     1981 gattgccgtt ttccctacac cgggttctcc tatcagaaca ggattatttt ttgtttttcg
     2041 tgaaagaata cggatgacat cacggatttc ttcatcacga ccgatgattg gatccatttt
     2101 tccgcttttg acttgctgta ctaaatcaac gccgtatttt tctaatgctt tgtattgttc
     2161 ttcttggttt tgtgaggtca ctctttctcc tcctctcatc tcttcgatgt ttttacgcaa
     2221 ttctttttca gataatccgt ttttattcaa ataaacagtt aacggataat tttttaattt
     2281 catcaacgct aaaataacga cttccgttga aagaaactcg tcgccgaagc tttcacgtat
     2341 ttggtctgct tcattcaata atcgaaagaa attctggctg aggttttgtc catattggac
     2401 gtttccacca gaaacaactg gatactcatc cagtagacga tcaatttcat gttcaaaaga
     2461 ttcgacatcc aatccggcat ccgtatagaa atttcgacca aaatgattag attgtaaaaa
     2521 gattttccat acatgggcaa tgtcgatatc ttgatgtttt cgagtgaccg caatctgttg
     2581 agcttcagca atcgcttctt gaagcgtcgt agtcattttc tcaatattca tacaggtata
     2641 ccctcctaat taaaggtaag atcacttatt tctttgatct ttacaaaaac tattatatcg
     2701 ctttggtcaa ttttggtcaa aatatacaca tagttttttg actttttttc tcgacttata
     2761 taaatcttaa aaaaaataaa attgattgta tgtatataaa aaaagagcca aacaaacgta
     2821 aaattatctt atccataacg tttt
//
LOCUS       DACEUF010000139         2842 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.145, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000139 DACEUF010000000
VERSION     DACEUF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.145"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            95..370
                     /locus_tag="IXG20_002559"
     CDS             95..370
                     /locus_tag="IXG20_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ1610717.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            808..1758
                     /gene="yidC"
                     /locus_tag="IXG20_002560"
     CDS             808..1758
                     /gene="yidC"
                     /locus_tag="IXG20_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1610718.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(1937..2137)
                     /locus_tag="IXG20_002561"
     CDS             complement(1937..2137)
                     /locus_tag="IXG20_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610719.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT          892 a          564 c          523 g          863 t
ORIGIN      
        1 ccatgattat atcatggaat aggtgcttga gcttgtttca tcctaaaaaa atcggtatca
       61 ttaatttata aaataggaaa gaaaggtgac gaacatgcgc aaagttaaaa tgaacgtcca
      121 aggacgcgtc caaggcgtag gatttcgcta catgaccaag atggtcgctg atcaactagg
      181 cgtgactgga acggtaaaaa acgaagatga cggctcagtc acgatcgaag caatcggcaa
      241 tgacgacatc atccagaaat ttattgaaga agtcaagaaa tctccgagcc caagtggacg
      301 agtgcagtat gtggatgtcc aagaagaccc gatgattgag gaacgaaaga aatttgatgt
      361 ggtgggataa ggttgtgaaa aagctggtct gcatcaagca ttaaggacag gttgtgaaaa
      421 atagctctcc atatttttca acaggctgga cttaatgccg tagatgcaag cttttgaaca
      481 ccgtcgataa ggttattctt gaggcgcttt gcctcaaaaa ttaaggacag cagtgaaaaa
      541 gctgatctat cacttgcagt gagttcattt gactccgctt tttcgggcag aaaaaaagtt
      601 tttagtttat taaagtaaga aagaaacact tggaatcatt ctactttttc ctagtatttc
      661 tttcttgatt ttttgtcttt ttttcaaaat ttttcgctac gtaaaagaac gaatatttat
      721 atgaaatttc tatgaagttg attgaattcc atgcgctttt ttagtatagt taagtttgtg
      781 taaaaattat agaagaggaa gtattctatg aataaatgga aaaactggtt attgggatcc
      841 ggacttgtca gtatcctgct ctttttatct ggctgtgtgc ggatggattc aaacggaaac
      901 cctgacactt cgggaatcgt ttatcgagta ctcgtccatc ctatgggcca agcgattaca
      961 tatctagttg aaaatttcaa ttggtcttat ggctgggcag ttattgcaat gactgtgatc
     1021 gtccgtatca tcatcttgcc actaggtatt agtcaatcga aaaaaacaat gatccaatct
     1081 gaaaaaatgc aagcgttaaa accacaagtt gaagcagccc aacaaaaatt gaaagctgca
     1141 acaacacgtg aagaacaaat ggcagcacaa gcagaaatgc agcaagtata ccgcgaaaac
     1201 ggattgagta tgaccggcgg aatcggctgt ctgcctctat tgatccaaat gccgatcttc
     1261 tctgccttgt actttacggc acgttataca gaaggtatcc gggaatcatc tttctacggc
     1321 atcgatttag gctcaccaag tatggtactt gttgccattg caggggtcgc ttatctgctt
     1381 caaggataca tctcaacgat cggtattcct gaagaacaga aaaagacgat gcgtacgatg
     1441 ctgatcgtct ctcctgctat gatcgtcttc atgtcgatca gtgcgccagc cggtgttaca
     1501 ctttactggg tagtcggtgg tgtgttcagc tgtctgcaaa cattcatcac aaacgttatg
     1561 atgaaaccac gtttgaaagc acaagtggca gaagagttga aacaaaaccc accaaaacaa
     1621 gtcgtcactc caagaattga tgtgacagaa tcagctaaac cagctgacag caaacaagca
     1681 accaaacaaa tcaagcaacc caaaaacagt acaaacaata aaggccgcaa cgcaggaaag
     1741 cagcagaagc ggaagtagat agtaaataag agtgtaataa aaattcatta acagttttag
     1801 caattaaaaa tatcgaccaa gatttttgaa aaagaaatct ggtcgatatt tttatttaag
     1861 tgaatgttta atttgaatca tcgattgatc gctatctaat tgcagcagca tctttttatg
     1921 aagaaacaac tttttcttat ctatcaacgt agacaggtgt tgctgtttct tctaatagtg
     1981 tacttaattc cttaatattt tgttttcctt cttctaacat ccaactactc gctttttctt
     2041 cgccttcggt aacaaaaggc gccactccct tcgcccatgt tgctcgccca cataagacac
     2101 cattctccat aaaggacaac gcggagatac cacccatcta aataacatcc ataaaccttt
     2161 ctagtagaag aatagtgata gtatccatcc ctttttctac tgggaataca attacacgat
     2221 tcaaagcttg atacacaaca tttcccttct tataaaccgc tattgtaacc gaattgtttt
     2281 gaatcacctt tttgggatag aaactggctc ctttccgcaa cttcttaaat gttggtttct
     2341 tttatacttt ctttgtagca actacaaatt tcttcaaatc tctaaatgtt tgcataacaa
     2401 ccgcctttct gacttttact caggatggta tttaaaaatc agcaaaataa ggcttctctc
     2461 catctttttt atttcctagc tgctttaaag acactcttgc tgttttttaa tctctatcaa
     2521 gggggctctg aatctatttt gatgaactta tcttattaat ttctcaaatg agctgtcaga
     2581 cacaactgta ttttttcaat ctcataccgg acaactatgc cagcttaaaa aaatttcctc
     2641 tggcaaaact tctttattga aaaatgacaa tgaaaaaaga ccattctttt tgttgaatgc
     2701 tcattgtgta aacatatttc ctgcgactgc tcttcgaacc attctcagtg caatccctaa
     2761 tttatctatt aaatcttctt ttagttcctc atctttcata ttcaaaattt cctttgtatg
     2821 ttacacaagt ttgttctcta aa
//
LOCUS       DACEUF010000140         2778 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.146, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000140 DACEUF010000000
VERSION     DACEUF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.146"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..197
                     /locus_tag="IXG20_002562"
     CDS             <1..197
                     /locus_tag="IXG20_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610720.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IXG20_002563"
     CDS             314..811
                     /locus_tag="IXG20_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1610721.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IXG20_002564"
     CDS             898..1290
                     /locus_tag="IXG20_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1610722.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="IXG20_002565"
     CDS             1274..>2778
                     /locus_tag="IXG20_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1610723.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DACEUF010000141         2773 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.147, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000141 DACEUF010000000
VERSION     DACEUF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.147"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            178..774
                     /locus_tag="IXG20_002566"
     CDS             178..774
                     /locus_tag="IXG20_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1610724.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="IXG20_002567"
     CDS             898..2697
                     /locus_tag="IXG20_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1610725.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMRGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          789 a          479 c          524 g          981 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcgtggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg ggatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagtagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt ttt
//
LOCUS       DACEUF010000142         2639 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.148, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000142 DACEUF010000000
VERSION     DACEUF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.148"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(36..1355)
                     /locus_tag="IXG20_002568"
     CDS             complement(36..1355)
                     /locus_tag="IXG20_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1610726.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1352..2005)
                     /locus_tag="IXG20_002569"
     CDS             complement(1352..2005)
                     /locus_tag="IXG20_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1610727.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2293..>2639
                     /locus_tag="IXG20_002570"
     CDS             2293..>2639
                     /locus_tag="IXG20_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1610728.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAIL"
BASE COUNT          875 a          415 c          264 g         1085 t
ORIGIN      
        1 tatttttcaa taactattgc aaaattacat cttaattaag aaaatatttt tattttaaag
       61 ataaaaaatt cttcatcctg caatacttta tattctatat tgtaattatt cagaatatta
      121 cttacaatat ataaacctaa tccattacta ttttccttat ttaaatcaaa tttaacatca
      181 aatattttat ccatattcga aattttattg ttaccgtatg aattctctat atataaccaa
      241 tcattaacta tcccaatatt aattacccca tttacatcag tatattttac cgcattacta
      301 atcaaattag atagaataat ctttaaagct gtttttccta tataaacatt ctcgtctaat
      361 atataattat tgacagttat tttcttttga tttgctaata tttcatactt ttttaatatg
      421 tcttctaatg tatcatttat tttcaaatat tcttcatcat tattcaaatt ttctatagaa
      481 tgaactgata atatttgaga aatatttttt gttaaactat caacgatttc aatacattca
      541 ttaatataaa tatctctttc tttatatttt ccaatattgt atttcatgtt ttccagtatt
      601 attttaagac tagcaagagg ggttttaagt tcatgggatg cacctttaaa aaagtcatat
      661 tttaactttt ctaattttaa aatttcttta tttttaaatt ccaaatcgtc aattgttctt
      721 aataaagtag aatacaaatc attaatttgt tgttttaatt ctccaacttc atcattagag
      781 ctaactttta aactcatttt tttatcaagt ttcatcattt tatcagtaac aatttttatt
      841 tcttgtatat tattttttat tgattttgca taaattaaag aaataatagc agaaaataaa
      901 attgatatta ataaagaata tggtaaaaat ttaaggctta aatctttcgc atctttttgc
      961 atatcagctg tagaaacaaa ttgtagattt gtttttttac catcattaag ctttatttct
     1021 ctttcttcaa ttatcaaaga attactatca ctttctaaat taacatttat attatctttg
     1081 atttgtattt cattcttatt atttttttct tttatgaata cttttatttc actactcttg
     1141 gaataaaact ccaaagtttg ttctatatat tttatttctt ttccattcat attactagaa
     1201 atttcatttg ccatattatg aatcttttct tttctcgtct ccaaatatgt tttaggaaag
     1261 ataaaaaaaa ttaatgaatg aactaatata attattattc caagaacaga aaatatttgt
     1321 atgaacattt ttggaaatat ctttaatttt ttcattttct ctccaattta tatcctacat
     1381 ttcttatagt agttatacag tctaattgta atttttttct aagttctttt atatatacat
     1441 ctattactcg atcataagga gtttcttcgc tatctttcca tacataatca ataatttgca
     1501 ttcttgttaa cacttgtcca ttattatcca ataaacattt taatacttct agttcttttg
     1561 catttacatc tatttcttca ccatttattt tagctgtata gctattaaaa ttaaccgata
     1621 gatccttata ttcaaacttc tctaaatgtc cataattctt cttaattaaa gaatctattc
     1681 tagcttttaa gaccggcaat gaaaatggct tttctacata tccgtctact aaattagtaa
     1741 atgcatcaat tttatattct tcatcactaa atgcagtcaa gattagaatt ggtaaattgc
     1801 ttttctttct aatttctttc aacacttcta aaccatttat aaaaggtatc tgaatgtcta
     1861 agataaccaa atttatatcg ctattaaatt ttgacaaggc ttctcttcca tctttagctt
     1921 gaataacagt atatccaaat tctgaaagat attcacttat tccctctctt atcatattat
     1981 catcttcaac agttaatatt ttcatatata cctccactta agcctataca ctagatatta
     2041 tacagcatat tatggctcta taatatctag tgttggtgac gccttctgtt gccaatactt
     2101 tttctttttt ttaataaatg caaacagaca ccttgaaatc ctttagaata aagggttcta
     2161 taatattttg tgttgttgga aaatcgaagg cgattttcta ctaacacttt ttcttcgttt
     2221 caactcaaaa agagactcac ttttggtaaa atttagtcga ccaaaacatc accctaccaa
     2281 aggagtcgct ctatggataa tcatactaga aaattactcg gattaacaga taaacattta
     2341 ttttttgatg aagaatggct gattgaaaaa gaatataaag gcgttcaagc gcagtttatt
     2401 aaagggagat tggatgactc accttcccat tgtgaacact gtggatcgat tcgtattatc
     2461 cgcaacggtt cctatacgac acgtactcag atattgaaag tcaaagaaaa gttgactatt
     2521 ttagaattaa aacgtacccg ttttctatgc tatgattgtg ggcagacctt ttctgcgaaa
     2581 acggacttag ttgatgaaca tcatcaacta acaaaggaac tgaagcaggc gattctcat
//
LOCUS       DACEUF010000143         2621 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.149, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000143 DACEUF010000000
VERSION     DACEUF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.149"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="IXG20_002571"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="IXG20_002571"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1610729.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          939 a          458 c          581 g          643 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg g
//
LOCUS       DACEUF010000144         2535 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.150, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000144 DACEUF010000000
VERSION     DACEUF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.150"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(51..1517)
                     /locus_tag="IXG20_002572"
     CDS             complement(51..1517)
                     /locus_tag="IXG20_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ1610730.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="IXG20_002573"
     CDS             1904..2500
                     /locus_tag="IXG20_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1610731.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT          884 a          495 c          440 g          716 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg aggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaa
//
LOCUS       DACEUF010000145         2445 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.151, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000145 DACEUF010000000
VERSION     DACEUF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.151"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(234..782)
                     /gene="aac(6')"
                     /locus_tag="IXG20_002574"
     CDS             complement(234..782)
                     /gene="aac(6')"
                     /locus_tag="IXG20_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1610732.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1335..2438
                     /locus_tag="IXG20_002575"
     CDS             1335..2438
                     /locus_tag="IXG20_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1610733.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT          766 a          475 c          428 g          776 t
ORIGIN      
        1 aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca
       61 aaatttttct tactactcga aactaagcga ctttttcaca acctctggta cacggtgttc
      121 caaaagtagc ttcttcggaa ataagttgga atttcacaaa aattcaagaa gtaattttcg
      181 taaattcctt cttatttctt gaagctgatt acttctgtca caacctcttt ttattattga
      241 gaatttggtc gaggaataat cgtttttgcc atccaaatat ccggtttgtc ccagccattt
      301 gcatttggta agacacctac gattttataa cctaattttt catagaattc atacggatgt
      361 tcacgaaggt tctggatgga agccacttta tcaaatgtat gctcatacag gtccgtttga
      421 cttaacgttg ttccatggtc taaatcatcc gtacctaaat aaatcgtgat tcctcctctg
      481 gaagctactt ctttttctaa gtaattgact aatcgagtac ctatttggtt ctttcgtcgg
      541 gagctttcta caactaatgg atgcaattcc caacctgtga taccgtattg agggattgca
      601 ccaataaatc ctactaactc atcttggtct accgctgcta ccgcgattcg ttctggattc
      661 atcatttctt ctacttcttc tgccgagctg tctccatatt cttccggcca agtcagtctc
      721 agtaaatcag aaagctgatc tttcaatact ggattattac ggtcaaattc actgattatc
      781 atacgtcttc ccccttgttc ttgcttattc taacagtcct tttgaaaaaa cgaaatgttc
      841 tcgctcaaaa atctttagct accaatcagt atctctattc tttcattatt tatcttactt
      901 ataaaataaa aaaattatac agaaacactt tattcaaaat aggaaagatc aggaaagcca
      961 tcaattggag gaaaaaactc tgtttgttca tcatgatata gattatcttt tgctttaaaa
     1021 aaaaccaatt atgaattgac aaataaaacc tgttttcata taataatctt aatttttaga
     1081 atttttagat aattacaaaa aagcaaaggg aatcggtaga aatactaaga ggaacaaaaa
     1141 ttcattattg tcgttttcaa tgtttttgta cttgttcgtt tttaggatac ttattgatga
     1201 tttcaaggtg ttcatgccac cttattcatc acttatcctg tcttaggttc acctctactc
     1261 catctacagc cgcagtcaga tcgatcactc tttaaaattt taacccgttt caacgaagaa
     1321 agaaggaaat gaatatgagt caaagtgttt tcttaccaaa ttataccatt ggaacagatg
     1381 cgtatgaaaa agcaaatgga atcgtcaaag aatatgggac aaaagtagtg ctgattggag
     1441 ggaagactgc actttcaaag gcagaaaaca aaataaaagt agctttgaca caggaaatag
     1501 aggttgtcgc cagtctctgg tacgggggta attcaactta tggaaatatt gaacagttga
     1561 tccagcatga aagtgtccaa aaagcagacg tcctgtttgc agtcggtggt ggccgtgctt
     1621 gcgatactgt aaaagtcatt ggtgaaaaac tagctaaacc agtcttcacc tttcccacta
     1681 ttggttcgaa ctgttcgccc gtcactgcgg tatgtgtcat ctataacgaa gaaggcgtaa
     1741 tggacggact cttctttcct tcaaaaccac ctgtacacac cttcattgat acagcaatta
     1801 ttgccgaagc acctgataaa tacttatggg caggtattgg cgatgcttta tccaaagaag
     1861 tcgaatctac tttttctgct cgtggcgatg atctaggcta cacggataga ttaggaatcc
     1921 aaatcggaaa aatgtgcatt gatcgtctct tgacttatgg tacgcaagca ttgaaagata
     1981 gtgccgacga tcgtccctca tatgcaattg aacaagtcgt ttcggatatc attgttacta
     2041 ccggattggt ttctgtttta gttaacaata actataattc tgctatggca cactcattct
     2101 attacggtac gactgttcta gaaaaaatgg agggatacct tcacggagca tctgtcagtt
     2161 atggtgtact gaccttattg atgatggatc agcagttcga tttgtttgat aaaatctatg
     2221 tgtttatgaa aaagaaccaa cttccagtat cgttaaccga catgaatctc aatgtcacga
     2281 ccgatttaga agcaatctta gataaagcca tggcaacaaa tgatattaaa catgtgcctt
     2341 atgaaatcac ccgagaaaaa tttaaacagg ccattcttga cgtagaaact tatcatgcta
     2401 cacaagaaag ccagcaagga tataagaaaa tcagttaaat aattg
//
LOCUS       DACEUF010000146         2415 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.152, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000146 DACEUF010000000
VERSION     DACEUF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.152"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..543
                     /locus_tag="IXG20_002576"
     CDS             <1..543
                     /locus_tag="IXG20_002576"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1610734.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1016..1588)
                     /locus_tag="IXG20_002577"
     CDS             complement(1016..1588)
                     /locus_tag="IXG20_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1610735.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1604..2209)
                     /locus_tag="IXG20_002578"
     CDS             complement(1604..2209)
                     /locus_tag="IXG20_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ1610736.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          761 a          475 c          371 g          808 t
ORIGIN      
        1 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
       61 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
      121 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
      181 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
      241 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
      301 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
      361 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
      421 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
      481 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
      541 taaccgtctc tactaaaatg tgtccaagat attgactcaa atccagtaat atcacaatag
      601 gaatgctgtt tattatatta gcagcagtgc tgatttttat gatagttatt tggttcttaa
      661 atgaaagatc taaagacaat caacaccgta gggatatgga gataaaatca atagagtcaa
      721 tagattattt atcgataata acactaaaaa agccacgttc ctaaacgtga cttacgctga
      781 acgatatgtg ttgtttggta gacgaacaaa tcgttcatta taaatatatg atggtatcct
      841 aatatagtaa gcgtggctag cataccttta attactttac cttttacagt taccatacac
      901 attttttgag cagaaatcaa gtaaataaaa aaatattata tttcacaaca tgctaataga
      961 ctcatatcta gaacgcatta agtatttacc tgtctggttc gctgagactt cttttttaac
     1021 tttcttcaat tttcttcttt attctaaaaa gcgtactgcg actgaaaccg gttatagctt
     1081 caacttcctt atacgattta cctgaagtaa gcagttcata tgcgtgcctc ttcttcggtg
     1141 tgattgttgc tttcggacgt ccttctctga aattaggatt attctttctt gcaaacattt
     1201 ttccttcttg ggtacgttcc acaatcatat cacgttccat ttctgctaca ctcaacaacg
     1261 tccgtgtcac cattcgcccc atggacgtat tttcaatcgt tcctagattc aaaaccttga
     1321 tcgttacgtt atcatcgagt aaaggttcaa tgatctctaa tgcttctctc gtgttacgag
     1381 caaatcgatc aagcttcgta acaactattt catctccagg cttcagacca gccaacagct
     1441 gattgaattt tggccgatcc cgttttgtac ccgtaaattt ttctgaaaaa atattcttct
     1501 tctctacgcc gttctcaagc aactgttgaa tttgttcttt tgtactttga gaagcggttg
     1561 agacacgtgc atatccatat ttcgtcattt ggtccaccta cttttacaaa tcatctatcg
     1621 tatactcaga atacccttca tagaaatagc tgacatcgat tcctttcata tataactcgc
     1681 gatcgtcaat tttatcagtc agagcattgg aaattagata gcgtatttcc aaatcgttta
     1741 tgggacttcg ctccattgca gaaagatagt ctgctttatc gattaagttc caatctacaa
     1801 ctttttgcaa ttcttccttt aaaatgagat ccagccaaat tctagtactt cggccatttc
     1861 cttctcgaaa tgggtgagcg atattcatct ccacatattt cttgataatc tcttcaaaag
     1921 aagtttgtgg catctgatca atatgctgta acgaatgttc taaatacata actggtgcaa
     1981 atcgaaaatt tccttttgca atattgaccg atcggatttt tccggcaaaa tcgtaaatat
     2041 ccgaaaatag aaattcatga atttcagata aacctttaaa tgtaccaact tcgatttcat
     2101 ttatttttcc acaatcgtac aattctttcg ctttttgttt actgagtttt tcttcaacct
     2161 tagccaactc aacttgattg gttaatccca atttattctc taaaatcatg cgaaatctcc
     2221 ttaactttat gacataagtt tatgaaacac tctcaacccc attatatcgc gattttgttt
     2281 atgtgtcaaa aactattaag tttctgaccc tcgatagatt tttcaagtgt ctctattttg
     2341 cacagtctag ttggttctgt tgcaaagttt taaataaaga ataaaatccc ttacggtatc
     2401 tatgatttaa gctgg
//
LOCUS       DACEUF010000147         2401 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.153, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000147 DACEUF010000000
VERSION     DACEUF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.153"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            208..2070
                     /locus_tag="IXG20_002579"
     CDS             208..2070
                     /locus_tag="IXG20_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1610737.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2070..>2401
                     /locus_tag="IXG20_002580"
     CDS             2070..>2401
                     /locus_tag="IXG20_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1610738.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          867 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaaatttg aaaaagcgtt tgattattta tattacgact tagtgcaata aaaaacgctt
       61 tttaacagta catgtagtcg tctttataat agataaactc tgtaatatta gttaaaaagc
      121 tataagttgc acttagttaa gtgcaaacat ttcatattgt cgttagttta gaaaacgctt
      181 atattagcaa tgtaaggaga gatgataatg cagcagctaa caaaaagaga aaggcagctt
      241 ttgatttact tggtggaaat gtctgaaccg gtaacaatca aaaagttggc tgaaaaagaa
      301 aatgttagtg ttcggacgat aaaatacgat ctagatgata taagagagtg gctgaatgag
      361 cggaagcaag tcctatatag caaacgtagt cagggaattt ggatgaatat tagtagctct
      421 gatcgaatca aattaaaaag cgaattgatg gatgttaatc gttttgaaat atttgcagat
      481 caaagtctcc gaatcaaacg tttactaatt atgcttttag taacaaaaga agctattact
      541 ggaatgaaaa tggccaacgc attagatgtc agcaaagata cgatcatgaa tgatttagat
      601 gtgctggaaa ataattttct taaggaagga ttgaccttga atcgtcaggc gcgaaagggt
      661 ttttggatca taggagaaga acgtctagtt cgattaacga ttgaagagat tcttcaaaaa
      721 gagttcacgg attatgacat ttataaatta atgtcattgt tattgaatgg cggtgagact
      781 gaatactttg aaatgtattc tgctactgct acacctatcc aagaggtatt taatcaagta
      841 atcattcgaa tgcgacactt actggaattt gaaaatctag agaaactgaa ttacgcggaa
      901 ttactaaata ttctgattcg agtgacaatt gctaccgttc gcttacgcaa agaggctacg
      961 attggtcgtt atcaattggt tgctgaacaa gaattagaca aaaacgatct ttcttatcgc
     1021 gtcatgcagc aggtctttga ttattatcag ttgccactgt tcgaggatga atatcattat
     1081 atctatagtg atacattcga cacgattcac cagcaagacg tcatgaaact agttgagaat
     1141 gtgatttata aagtaagtaa agcaatcaac cattcttttt atcaagatcc acaattgttg
     1201 acgaatctgt atgctcattt atccatgcga ttatcgagaa agaaaaaatt tgtcaatgaa
     1261 tataatcctt ttaaagaaga tatcaagcag aaatacccgg aattatttga tgcgatcaag
     1321 caagcagttc gacaagaaat aagtgaacaa aatattctga tcaatgattc tttcgtggca
     1381 tatattgcct tacacttttt ggtttcctac gaaagagaag aagatctacg aagcgttaga
     1441 gccgtctaca tttgttcaac agggttgggt gtgactagtc tgatcaagca aaagatcaat
     1501 gaagaattaa ataatatcac tatcgtggca tttgcttcag tattgaatgc gcaagaaatg
     1561 attgctacca aagatcctga tttggtgatt agtatttttc caattaatgg catcgatcgt
     1621 cctttcatca aagtccatcc attacccact gaacaggact tgaaaaaaat tcaacaaata
     1681 ataaaaaaaa tacttgcttc ttccaatcag caggaaaggc ataacttgaa tcttagagaa
     1741 acgtccatca ataaagatac tttagaagac tttagcaagg aattgatttt gaaagcttat
     1801 acgatttatg aacgtttact tgtcattttg gaagcagaat tgatccaaga atatcgagaa
     1861 gcttttttgc ttcatgtatt cttaatggtt catcgtatca catttgatca acaatatatg
     1921 gcagaaggtc gtcaagtaga ccagattatt ttagacaaag gaacactggt aggaaaaatc
     1981 gaagcagttt ttgcagaaaa taatttagga atcaatcagt cagaaatcag tgcattattc
     2041 tcatatataa aagaaggagc gtcaagttag tgaaaatgtc agacgaggct ttaaaaatta
     2101 tcgaggaaag tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt gaagagcgat
     2161 tagtagaaca tgctattgta ccaaccgagt tgcagtggac cattctaatt aatcacttga
     2221 atgagatgct gaaacgttct caaagaaagg aaacgattcc cgtagtcgat cgggatttgt
     2281 tcaaagaagt atccaaagaa gccataacta tttctgatga aatagttaga agtatcggta
     2341 atttaaccga tgatgaaata tatgttttgt cgattcattt tgaaacagca aaaaataatt
     2401 a
//
LOCUS       DACEUF010000148         2293 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.155, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000148 DACEUF010000000
VERSION     DACEUF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.155"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(51..1598)
                     /locus_tag="IXG20_002581"
     CDS             complement(51..1598)
                     /locus_tag="IXG20_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1610739.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IXG20_002582"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IXG20_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ1610740.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="IXG20_002583"
     CDS             complement(2043..2237)
                     /locus_tag="IXG20_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610741.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          644 a          476 c          408 g          765 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ttcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DACEUF010000149         2202 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.156, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000149 DACEUF010000000
VERSION     DACEUF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.156"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(581..1195)
                     /locus_tag="IXG20_002584"
     CDS             complement(581..1195)
                     /locus_tag="IXG20_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1610742.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1801..2178)
                     /locus_tag="IXG20_002585"
     CDS             complement(1801..2178)
                     /locus_tag="IXG20_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610743.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT          733 a          420 c          326 g          723 t
ORIGIN      
        1 tttttcctta aaattctttt tttattttta gattagagaa aatctcgtcc tgtttttttc
       61 attttacaat cattgaaaaa ttgaatttca ttgcaaaaaa aataataaac acttataaat
      121 atccattatc tatccttcaa tttttgtgta ttttatttct attagaaaaa ggtatcctgt
      181 tggacaattt tgttatatca ggtaaacttc attgcaataa tgataacaaa tgtactataa
      241 ttactttgta aagagttttg gggaagactc tttcatcaac gtgtattaag agacaagaaa
      301 gaatccatat ggattctttc ttgtctcttt ttccactact ttttattttt ctgacactac
      361 atattcttat aactcatttc tcataactgt gaaacaactt ataaaaataa caaactttaa
      421 acaaaacaag cttaaaatca aagatgatcc atcttaaaat aactaaaaca aaaaaagtag
      481 tcgttgttaa tcctcattaa taatcgtttt ctttttttca aacgctccat ttttatgtgg
      541 aacattattt ttttaaggaa ctttgtcaac atgtggataa ctattttcca cttgtgtcct
      601 ggactagctg ttttgctgta aaatacaaaa cttgctgcag caaaaagatc gttgcaagag
      661 aggctgttcc gcagatcgct ggatctagat ttggcaaagt caagactgca tcatgtaaat
      721 tggcgtggtt gtccaaaacc aaatcagcaa attcaaataa tcgcaaacca ctgctatgtt
      781 gcgatttgat acttcgtgac aaatcaaatc cagtaacaat gattaatgga tgaccatttt
      841 ctttgatcca ctgagctagt tcgatgattg ccgggtttct tccttcattc gaaagaagaa
      901 gaaaaatgtc ttctgatttg acagaaagct gatcaataat tgcctgtcct gcaccttcta
      961 gcgtttcata gacacctccg gctggatttt caatcaatat acttggtaca agatccgggg
     1021 tttgatccca aaattcaaat gctacaccac ttagtgtgcg gctagcatat atctgcaatc
     1081 taccaccttg attaatttgt ttcgcgatcc aagtgccagc ttgaagcatc agcggctctt
     1141 ctatccttaa tttctcttcc aattggtgat aatagtcaaa ataacataaa ctcatttatc
     1201 tctcccgcct agctatactt gacatgaata gaaacagctg cctaatctaa ggaagtacat
     1261 aaaaaaacag tagttagaca gtaacacatg tggtctttgc tgtttcaaac acaaaaaagg
     1321 cattgaacga atacgcccaa taccttgaca tagcaaataa aacaacgtgc agaccatgga
     1381 aaatatctac ataggggtag aattttcacc ctagattagg tacaataaat taggtcgatg
     1441 cactgcattg actatggcaa aatgatcggt cagggtgtat gtgtgtgact ggtcattccc
     1501 tgattcttag cgccaactaa gggtcaggga gccttttttt attcagctgt ttctatctga
     1561 atttattgta agtaatctct tatccaagtg caagcaagaa aatacaaaaa aacattggta
     1621 tgatttatat atatataaat aacataaaaa aaggaatggc ttatcccgat acaacaagca
     1681 ttcctttaaa aaagattctt ttatataagt gaaaaaagct tttttccacc aattaaataa
     1741 atctctcatc tcaatagttt atccattatt ttatcttttt ttgcgtatac ctactaaata
     1801 ttaccgtctt cttctcgttc ttctactttg ttcgagagaa tctgtactag aaaaagtagt
     1861 tgtatgtcta ccttttctgc ctatccgaga ctttcttttc atatatggga tataaatgat
     1921 tagatacagt acagcaaaaa ataaagcaaa tacgcaagct tcccaactac ctaacgccaa
     1981 ttcaaccatc aaggtagcaa ccccaacgga tgctgcatac cagataaaaa tcagaaacgt
     2041 ttcttttttt ctgaccatgc gaaacatgat aaatagacta ataagcaata gtggcaacac
     2101 aaatatccaa ttgcttcctt ccatcagcca cttgagtata tcaagcagcg actgtgcaac
     2161 agcgactaat tttcccatag tgaacacaca ccttttttta ta
//
LOCUS       DACEUF010000150         2125 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.157, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000150 DACEUF010000000
VERSION     DACEUF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.157"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..159
                     /locus_tag="IXG20_002586"
     CDS             <1..159
                     /locus_tag="IXG20_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1610744.1"
                     /translation="RRNKFYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
     gene            242..859
                     /locus_tag="IXG20_002587"
     CDS             242..859
                     /locus_tag="IXG20_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610745.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            1023..1355
                     /locus_tag="IXG20_002588"
     CDS             1023..1355
                     /locus_tag="IXG20_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610746.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            1679..1840
                     /locus_tag="IXG20_002589"
     CDS             1679..1840
                     /locus_tag="IXG20_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610747.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            1872..2125
                     /locus_tag="IXG20_002590"
                     /pseudo
     CDS             1872..2125
                     /locus_tag="IXG20_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          758 a          272 c          429 g          666 t
ORIGIN      
        1 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      241 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      301 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      361 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      421 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      481 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      541 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      601 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      661 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      721 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
      781 acgtactatt ttaaaatttg agcaaacctt tagaagtttt ttgaaaaata gagtatccca
      841 aatttttaat ttggattgaa aaaattaaaa atttggccaa aaatactaca gttttttaga
      901 gcaatcatct tatgatgatt gctctttttt tgttgttttt gtgtcattcg ttgttcaaat
      961 cctctattcg taaaataaat attttaaact agaaatatgc cttattttta ggaggaatca
     1021 aaatgaacaa tagagaaata ttggcaaaag aaatggcgga tttgaaagca aaagaagcga
     1081 agattagagc gaaacgaaaa cagattagac agaaagaaat gatttcttta gcgaaattat
     1141 attgtaaaaa ggaaggcttt aagacacctg ctgaagcatt agattttttg gaatcaaata
     1201 aaagaaatca ttcagtaaat gatgaggttt tagtatcgct aagaaaattt gcagatggat
     1261 tagtttggca agaaaatggt gcatattggc gtacgaatga tgttaagggt ttgactcaat
     1321 ttatggcaca atttcgttca gaaaatgtga actaaattga ggaggggttg aagtgaaaag
     1381 caatactttt cacttttggt tttttgcgga ataaaaattg tgaaaacaat gacgcaaaaa
     1441 gccaatggtt ttgaacgaaa aaaggacgta aaaaaggaat gcgaaggcag tcgtttttcg
     1501 ttcgcttttt cgtttgagag aaacggagtt tctcatcggc tgttaagccg atatagccct
     1561 cggcagagct tacagacttt acgcagtata agtctagtaa gttatacctt acatggaggt
     1621 ttttccgaat ttagcttgtg attctcgcaa taattttaac gaaaggaagt acacagttat
     1681 ggcctatatg gtggcacgaa cacaaaagca taaagttggg tcgttaaatg gacttcaaaa
     1741 tcatgtagat cgtaaaacga aaaatcatac gaataaagac attgatatta cgaaaagtgg
     1801 ttctgttgca aagttttcca aaaaatctat tttagtgtaa aattgagaaa aaagacagag
     1861 aggatagagt aatgaatcat tttaaaggca aacaattcaa aaaatgacgt cattattgtc
     1921 gctgttagtt actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat
     1981 gatcgtggaa taaatgtttg tcatactacg atttatcgtt gggtgcaaga gtacagcaaa
     2041 gtcctcgatt atctttggaa gaagaaaaat agacaatcct tctattcatg gaaaatggac
     2101 gaaacctata tcaaaattaa gggac
//
LOCUS       DACEUF010000151         2056 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.158, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000151 DACEUF010000000
VERSION     DACEUF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.158"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            493..714
                     /locus_tag="IXG20_002591"
     CDS             493..714
                     /locus_tag="IXG20_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610748.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="IXG20_002592"
     CDS             715..972
                     /locus_tag="IXG20_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610749.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="IXG20_002593"
     CDS             complement(1108..1710)
                     /locus_tag="IXG20_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1610750.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="IXG20_002594"
     CDS             complement(1777..1932)
                     /locus_tag="IXG20_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1610751.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          464 c          313 g          601 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggcggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaat cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatca ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DACEUF010000152         2052 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.159, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000152 DACEUF010000000
VERSION     DACEUF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.159"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            161..271
                     /locus_tag="IXG20_002595"
                     /pseudo
     CDS             161..271
                     /locus_tag="IXG20_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(368..844)
                     /locus_tag="IXG20_002596"
     CDS             complement(368..844)
                     /locus_tag="IXG20_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1610752.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(857..>2052)
                     /locus_tag="IXG20_002597"
     CDS             complement(857..>2052)
                     /locus_tag="IXG20_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1610753.1"
                     /translation="GFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLG
                     FFGGSRFIPIVSSLAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRL
                     TGAVGLHHTIYPLFWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFA
                     GRFATMMFGLPAACYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAP
                     WLYVIHAFLDGLSFYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIA
                     WAFIYFFVFTFFIKKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENI
                     ESVEACATRLRVAVTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQI
                     LGKDN"
BASE COUNT          726 a          384 c          283 g          659 t
ORIGIN      
        1 catctttcag cattttacta tatttggtat atagtgctaa ggttcatatt cgattgacaa
       61 gagaatataa attgatctat ttctatttcc taagttgagt aaacataact ttgcccttta
      121 taaatatctt ccctctctct cttctctcta tattttatat ttgaataatc atatcaaggt
      181 attccaacga aatgccaatg gatttcgaag cttttataac tttaaattac ggatcatgat
      241 ttggcatggg gaatcattac taataaaata aaaaaatgaa cagaaagaaa acagttcttt
      301 ctgttcacca acttcatttg acaaatatcc tatttagctt attttatagc tttttatata
      361 cactttttta aaaagtgatt tttccacatg aattcccatg agcaattcga ccattatcta
      421 ttattaaatc attgaactga tttttctccg gaaaaacaac tataacagaa gtatctttac
      481 cagattttat tattttgtca aaatccacat caaccaacga ggtttcagga gtaacctcat
      541 cattttcttg aacacaaatc gaaaaaggct ctccttgtaa ttcaactgta tcaattccaa
      601 tatgaatcaa ataatcaatg cctgagttgc tttttatatt aatagcatgt ttagtaggaa
      661 atatatttgt tactcgacca gccaatggag cataaatttt tgaaccatca ggtatgacgg
      721 caaaaccatc acctagtaat ttttgtgaaa agacaggatc atttacttct gtgatggaaa
      781 tgatttttcc ggaacaaggt gcataaaaag tttcttgttt tttctttttg aaaatttcaa
      841 acatattaaa aacctcctaa ttatcttttc ctaatatttg attgatttct tgacttaata
      901 agtcagcttt ccctccaaaa acagcttgga tgccaccctt cacttcgaag acagcagttg
      961 caccagtgtc ttttattgtt tgtttttgaa taatctcccc attcgtaaca gcaacacgca
     1021 aacgagttgc acaagcttca acagactcaa tattttcttt tccaccaaga gcttcaataa
     1081 tgataagaga ctcttcatgt aatgatgtat ttttgtttat tactttttca gaatgaactt
     1141 ctgattcttc ttccattcca ggaatagcta ccttaaattt tttgataaag aatgtaaaaa
     1201 caaagaaata tataaatgcc catgcaatac caaaaggaat taattttaac caattagttt
     1261 tgtcattccc ttgaagtaca ccgaataata aaaagtcgat caaaccacct gaaaaggaat
     1321 tccctacacg aatatttaag atatcagcaa aataaaaaga taatccatct aagaatgcat
     1381 gaatcacata aagccaagga gcaacaaata aaaacatata ttcaactggt tcagtaattc
     1441 cagttaaaaa tgatgtaagg gccccgctaa aataaagccc accattcttt ttacgatttt
     1501 tcttaggaat tgcacgatac attgcataac atgctgctgg taatccaaac atcattgttg
     1561 caaatcttcc tgcaaaaaat ctagtaccat aagtaaacag accagtgtgg tttggatcag
     1621 ctagttgggc aaagaaaata ttttgggctc cagcaattgt tgatccagct acagtttcgg
     1681 taccacctaa agatgtatac cagaacaaag ggtaaatggt gtggtgtagt ccaacagcac
     1741 ctgttaaacg caataaaaaa ccatataaaa aagttcctaa actacccatt tgggcaattt
     1801 gttcaccagc aactactaaa ccattttgaa ttggtggcca aattaaataa aagaaagaac
     1861 caataatgat tgcagctaaa ctagaaacaa tcggaataaa tcttgatcct ccaaaaaaac
     1921 ctaagaattg tggtaattca atttttctat aacgattgtg taaaaatgca acagtaccac
     1981 caacaacaat tgatcccact acacctgtat ctagtgttgc atcttttgct gaaaaaagtt
     2041 caagaaaccc ac
//
LOCUS       DACEUF010000153         1863 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.160, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000153 DACEUF010000000
VERSION     DACEUF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.160"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..168
                     /locus_tag="IXG20_002598"
     CDS             <1..168
                     /locus_tag="IXG20_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAQ1610754.1"
                     /translation="GFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGVRAYATCNAT
                     LTGNTGWWNVY"
     gene            222..1301
                     /locus_tag="IXG20_002599"
                     /pseudo
     CDS             222..1301
                     /locus_tag="IXG20_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1411..>1863
                     /locus_tag="IXG20_002600"
     CDS             1411..>1863
                     /locus_tag="IXG20_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1610755.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSR"
BASE COUNT          723 a          285 c          234 g          621 t
ORIGIN      
        1 ggatttaaag tttatgcaaa ttactaccat gctagagcta aacatagtgc aacagctaaa
       61 aatggacgag gcggtcaagt acgatcagtt cagaaagctg gagtaagagc ttatgcaaca
      121 tgcaatgcca cacttactgg aaatactggt tggtggaatg tttattaaaa aagagagttt
      181 aaactctctt ttttatttac atttgaaagg aaattcaaaa tatgttaaaa aaaataaacg
      241 ctgttttatc actttttgtc tgcattacaa tactattttt ttctactaat gttataaatg
      301 gtataaagac ttctgaagcg ctatataaaa ataccgctga aattcatatg caatataaag
      361 atgatatgga ctttaacagt tatataaacg ctttgtatga aatatctaca caagaaaata
      421 ttaatatttc tcaatatagt tttactgctg ttaaccaact aactattctt gctacaaacc
      481 ctaacgctaa caaacactgg aaattttcta aaaaaaatat attagcaaga aacgattcta
      541 aagtccatta tactaataac aaaaattcta agtaccaatt aaaacttcca aataattttt
      601 taaatataaa tatttatcct ttttctcatg tggaaaatat tggattaagt gaaatattat
      661 atgtacaagg aaactcaaac aaattaattc ctgtactgaa aaaatatgga acacctacta
      721 tttccaatat caatcaatca gatacttttc aaattaatac taatattgtt cttgttatct
      781 gctatcttag tatatttatt gtaattacta caattatatt tgctttttca aaaatgaaag
      841 aaatcactct aaaaaaaatg ttaggctata actcttttga tactataaaa gattctatta
      901 aagagttaaa cctcttattt tgttctttga ttataacttt cattttatct atttttttga
      961 gcatatatta cagtaacgaa aattttttcc tatcatttct tctatgtagt atttttcatt
     1021 ttataattag ttttttattt atcatagtta ctctaagtat ctatcattta tctagtataa
     1081 aaaattatct aaaaaattca cgaccactta agttaaatct ttgtatatta aatatctgtc
     1141 tattcttaag catgatcctt ctgttaatag cttctacaaa agtaataaat acccataatg
     1201 aagccgaaaa taattctctt aaatattggg aaagaactac taatttatac aaaacaaata
     1261 ttactaatca attaaataga aacaacaccg tagaagaaaa taagagagta taaaatattt
     1321 tgtgtaaatg aaaaaatcca tacaaaaaag gaagtccctt ctgtagaata aagttaacga
     1381 caaccaattc acagaaaaga ggacttccct atgaatgatt ttactacaga aattgtgcaa
     1441 actctagtca ctaaaggcga tttaaatgaa ttattccgtt cgcacttaga aaaagcgata
     1501 aacacactcc tacggactga attaacggct tttttagatt acgaaaaata tgatcgcact
     1561 ggttttaatt caggtaattc gagaaacggt tcttactttc gatcaatcaa aaccgaatat
     1621 ggtgaattaa cattggaaat acctagagat cgtaatggtg agtttaaaca acaaacttta
     1681 ccagcctaca aaagaacaaa cgatacattg gaaaccacta ttatccattt attcgaaaaa
     1741 ggtgttacga tgtctgaaat tgctgatttg atcgaaaaaa tgtacggtca tcactatact
     1801 ccacaaacca tgtccaacat gactaaagtt ctgactgaag aagtaaatgc ctttaaatcc
     1861 aga
//
LOCUS       DACEUF010000154         1852 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.161, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000154 DACEUF010000000
VERSION     DACEUF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.161"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(493..1785)
                     /locus_tag="IXG20_002601"
                     /pseudo
     CDS             complement(493..1785)
                     /locus_tag="IXG20_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          577 a          371 c          310 g          594 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tgtacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctca ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga tt
//
LOCUS       DACEUF010000155         1778 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.162, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000155 DACEUF010000000
VERSION     DACEUF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.162"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            1..72
                     /locus_tag="IXG20_002602"
     CDS             1..72
                     /locus_tag="IXG20_002602"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1610756.1"
                     /translation="MCSLLVGLLSALFSYWLNNRNKK"
     gene            complement(374..619)
                     /locus_tag="IXG20_002603"
     CDS             complement(374..619)
                     /locus_tag="IXG20_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610757.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            965..1249
                     /locus_tag="IXG20_002604"
     CDS             965..1249
                     /locus_tag="IXG20_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610758.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1252..1668
                     /locus_tag="IXG20_002605"
     CDS             1252..1668
                     /locus_tag="IXG20_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610759.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
BASE COUNT          614 a          324 c          267 g          573 t
ORIGIN      
        1 atttgctccc ttcttgttgg gcttttaagc gctttgttca gttactggtt gaataaccga
       61 aacaagaagt aaaacttaat taaggcgact cagcccacaa aaaaagcccc agagaatccg
      121 acctctctgg ggctttcgtg tgtttcgtgt tcacttattt gcaatcaaat tataacagaa
      181 ataaatattt atttcaaaaa tatcctacaa aagtctaaaa tccgtaatta tttcttatat
      241 gcaacattta caaataaagt cgctctgaga gctcgtattt ttcgatttaa gacgttttat
      301 ttagtttttg gtgtatttca ttggtccaag ctaaaaaact cttagaagcg attttagagc
      361 catttaaacc cacttaatta tattctccat gaaattcgct ccatgcgtca aaagctgcgt
      421 ctgaaattcc gccgtcttcg ttcactagtt gcattaattt ttccaacgat cgttttcttt
      481 cgggtgtcat atttttatat tccaacaatt tttcgtctgt taaatcgaac ttgattgctt
      541 gaattgcttg ctctaatttt tctaagtttt gaaaatattc gtaaggaaat ttttccattt
      601 tttctttctc cagtctcaca atttttctag aaataaaatc acttctttgc cgtctatact
      661 ctcaaaaaaa atgtcgtttg aaaaactaaa taaatttttg atcgtgcatt tttaaatttg
      721 gttatcgatt acacgtgcag cactcccccc ccgaatttta aagagcataa ggaaacgtgt
      781 acccactttc taaaaaaagt gggtagatag gctgtgccta tcacgtttct cagaaaaaaa
      841 tttgatatca ctttttcgcc atcgtgggta gcttatgtat agcattttgg caaaaaacag
      901 ctatacaaat atagtatttg tatggtatac ataaattata cataattgca aaaggagatt
      961 ttttatggac attttagttc ggaatattga tccaaagttt gttgcaacaa tagacaaacg
     1021 ttgccaagaa ctcacgaata aaacaggaga aaaatggagt cgaaataaat atctaaaagt
     1081 tttaatcgaa agtgattttg accgtcaact tacgacttac aaaaaagacc aattcgattt
     1141 attgctcgat aaatttattg ctatccaaac agaaaatact caaatattaa gcgagtatgt
     1201 ctacacaaat aatcaaatca ttgaaatgct tatcggagga atgatataac catgagaaaa
     1261 acttctgtcc aaaaacatgt tactttgtac cctagtcatg ttgacgtttt agattccatc
     1321 atcgaaaaaa atccagatat caataactac tcggaagcca ttcgcttttt atgtatgagt
     1381 tatgaacccg ataccaacac gaccactacc agcaatcagg caaaactaaa tgctatggga
     1441 aaagaaattt ccatcatgac tgaaatcatc gcagaatttg ctgatttata tctaaaagat
     1501 acaggcgttc ttattggcac aaattctaaa gtatatcaag aagtgaaaga aaaaatcgaa
     1561 gctaaactaa atccatcaaa tcgaaatcga ccaaagaatc atcaaaaaga aaccagtcaa
     1621 acagtcactt ctagaaattt cgacccaaca aattttctaa aacgataaaa aacagatagg
     1681 agttattcct atctgttttt tatttagaag ccccatacaa tatgctagaa tgtactagca
     1741 atcttagtcg gttgtaaggg acttgtctac ttgcttaa
//
LOCUS       DACEUF010000156         1677 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.163, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000156 DACEUF010000000
VERSION     DACEUF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.163"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(51..122)
                     /locus_tag="IXG20_002606"
     tRNA            complement(51..122)
                     /locus_tag="IXG20_002606"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(87..89),aa:Glu,seq:ttc)
     gene            complement(134..207)
                     /locus_tag="IXG20_002607"
     tRNA            complement(134..207)
                     /locus_tag="IXG20_002607"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(171..173),aa:Ile,seq:gat)
     gene            complement(220..290)
                     /locus_tag="IXG20_002608"
     tRNA            complement(220..290)
                     /locus_tag="IXG20_002608"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(256..258),aa:Gly,seq:tcc)
     gene            complement(295..367)
                     /locus_tag="IXG20_002609"
     tRNA            complement(295..367)
                     /locus_tag="IXG20_002609"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(332..334),aa:Phe,seq:gaa)
     gene            complement(371..443)
                     /locus_tag="IXG20_002610"
     tRNA            complement(371..443)
                     /locus_tag="IXG20_002610"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(408..410),aa:Asp,seq:gtc)
     gene            complement(462..535)
                     /locus_tag="IXG20_002611"
     tRNA            complement(462..535)
                     /locus_tag="IXG20_002611"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(499..501),aa:Met,seq:cat)
     gene            complement(548..637)
                     /locus_tag="IXG20_002612"
     tRNA            complement(548..637)
                     /locus_tag="IXG20_002612"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(599..601),aa:Ser,seq:tga)
     gene            complement(660..731)
                     /locus_tag="IXG20_002613"
     tRNA            complement(660..731)
                     /locus_tag="IXG20_002613"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(696..698),aa:Glu,seq:ttc)
     gene            complement(753..840)
                     /locus_tag="IXG20_002614"
     tRNA            complement(753..840)
                     /locus_tag="IXG20_002614"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(804..806),aa:Ser,seq:gct)
     gene            complement(865..938)
                     /locus_tag="IXG20_002615"
     tRNA            complement(865..938)
                     /locus_tag="IXG20_002615"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ile,seq:gat)
     gene            complement(952..1022)
                     /locus_tag="IXG20_002616"
     tRNA            complement(952..1022)
                     /locus_tag="IXG20_002616"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(988..990),aa:Gly,seq:tcc)
     gene            complement(1024..1099)
                     /locus_tag="IXG20_002617"
     tRNA            complement(1024..1099)
                     /locus_tag="IXG20_002617"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1064..1066),aa:Asp,seq:gtc)
     gene            complement(1118..1191)
                     /locus_tag="IXG20_002618"
     tRNA            complement(1118..1191)
                     /locus_tag="IXG20_002618"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1155..1157),aa:Met,seq:cat)
     gene            complement(1204..1293)
                     /locus_tag="IXG20_002619"
     tRNA            complement(1204..1293)
                     /locus_tag="IXG20_002619"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1255..1257),aa:Ser,seq:tga)
     gene            complement(1317..1390)
                     /locus_tag="IXG20_002620"
     tRNA            complement(1317..1390)
                     /locus_tag="IXG20_002620"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1354..1356),aa:Ile,seq:cat)
     gene            complement(1404..1477)
                     /locus_tag="IXG20_002621"
     tRNA            complement(1404..1477)
                     /locus_tag="IXG20_002621"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1441..1443),aa:Met,seq:cat)
     gene            complement(1505..1577)
                     /locus_tag="IXG20_002622"
     tRNA            complement(1505..1577)
                     /locus_tag="IXG20_002622"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1542..1544),aa:Ala,seq:tgc)
     gene            complement(1597..1670)
                     /locus_tag="IXG20_002623"
     tRNA            complement(1597..1670)
                     /locus_tag="IXG20_002623"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1634..1636),aa:Pro,seq:tgg)
BASE COUNT          464 a          457 c          394 g          362 t
ORIGIN      
        1 aaatcattct ttcagataaa aagaaaaaga tactcaaaaa gtatcttcca tgacccgtac
       61 gggattcgaa cccgtgttac cgccgtgaaa gggcggtgtc ttaaccgctt gaccaacggg
      121 ccaataaaat aaatgggcct aaatggactc gaaccatcga cctcacgctt atcaggcgtg
      181 cgctctaacc agctgagcta taggcccata aaaactaaaa gcgggtgacg agaatcgaac
      241 tcgcgacaac agcttggaag gctgtggttt taccactaaa ctacacccgc ataatggcgc
      301 gggacagaat cgaactgccg acacatggag cttcaatcca ttgctctacc aactgagcta
      361 ccgagccaaa cggttccgac gggaatcgaa cccgcgatct cctgcgtgac aggcaggcat
      421 gttaacccct acaccacgga accaatgttt ttagttttta attgcgggag caggatttga
      481 acctacgacc ttcgggttat gagcccgacg agctaccaga ctgctccatc ccgcgataat
      541 atgaaaaagg aggataaggg attcgaaccc ttgcacggtt ttacccgcct gacggttttc
      601 aagaccgttc ccttcagccg gacttgggta atcctccatg taaaactata ttaaattaat
      661 gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg
      721 accaacgggc caggtaaaac aaaatattga aacggagaag gagggatttg aaccctcgcg
      781 ccggtttccc gacctacacc cttagcaggg gcgcctcttc agccacttga gtacttcccc
      841 aaaaccaata agattttgta ctaatgggcc taaatggact cgaaccatcg acctcacgct
      901 tatcaggcgt gcgctctaac cagctgagct ataggcccat aaaaaactaa aagcgggtga
      961 cgagaatcga actcgcgaca acagcttgga aggctgtggt tttaccacta aactacaccc
     1021 gcatggcggt tccgacggga atcgaacccg cgatctcctg cgtgacaggc aggcatgtta
     1081 acccctacac cacggaacca gtgtttttag tttttaattg cgggagcagg atttgaacct
     1141 acgaccttcg ggttatgagc ccgacgagct accagactgc tccatcccgc gataatatga
     1201 aaaaggagga taagggattc gaacccttgc acggttttac ccgcctgacg gttttcaaga
     1261 ccgttccctt cagccggact tgggtaatcc tccatatgaa acttacaacg ccacaatgga
     1321 ccttgtagga ctcgaaccta cgaccggacg gttatgagcc gtctgctcta accaactgag
     1381 ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc gaacccccga cctcccgggt
     1441 atgaaccgga cgctctagcc agctgagcta caccgcgaaa aagatattca ttatttaaat
     1501 gaaatggagc ctagcgggat cgaaccgctg acctcctgcg tgcaaagcag gcgctctccc
     1561 agctgagcta aggccccgtg tttaaattta taattatcgg gaagacagga ttcgaacctg
     1621 cgaccccttg gtcccaaacc aagtgctcta ccaagctgag ctacttcccg tttaatt
//
LOCUS       DACEUF010000157         1664 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.164, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000157 DACEUF010000000
VERSION     DACEUF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.164"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            131..505
                     /locus_tag="IXG20_002624"
     CDS             131..505
                     /locus_tag="IXG20_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1610760.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IXG20_002625"
     CDS             complement(619..1248)
                     /locus_tag="IXG20_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1610761.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLPLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IXG20_002626"
     tRNA            complement(1382..1454)
                     /locus_tag="IXG20_002626"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          284 g          556 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt aggggaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DACEUF010000158         1629 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.165, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000158 DACEUF010000000
VERSION     DACEUF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.165"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            287..694
                     /locus_tag="IXG20_002627"
     CDS             287..694
                     /locus_tag="IXG20_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610762.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IXG20_002628"
     CDS             708..1109
                     /locus_tag="IXG20_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610763.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IXG20_002629"
     CDS             1111..1482
                     /locus_tag="IXG20_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610764.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          612 a          209 c          283 g          525 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtataca
//
LOCUS       DACEUF010000159         1611 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.166, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000159 DACEUF010000000
VERSION     DACEUF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.166"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            244..1494
                     /locus_tag="IXG20_002630"
     CDS             244..1494
                     /locus_tag="IXG20_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1610765.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          534 a          319 c          283 g          475 t
ORIGIN      
        1 atttcctcct gtagcgtttg tctaccctgt ttagtctgtt acccgaacca ctgttatcac
       61 taccaactca ttagctgttt cgataatgga ctagactttc aaatagtagt ttggaaggca
      121 gcttctagct cttttatcac cgtattctat taggcgaggt tgtggctata cagtgagttt
      181 ctgggaaatc ccacagtatc gaagcatttt ccaaaattta tatcgaaagg aggtccttct
      241 tccatgaaat tatttgtcgg tattgacgtt agctctgaaa aactagatgt ttgtttttta
      301 acagacgaca atcaattgtc tatcttatct gaaatctctg ttgccaatga catcgaaggt
      361 gcttcattca tacgagaaac aattcttgaa ttcaataaca gctaccactt tgatcaaatt
      421 gtgattggca tggagtcaac gtccatgtac agcttccatc cttccatgtt ttttcatgaa
      481 gatgagaaac taaagaagct caacacactt gtaacgattg aaaatccttt tcgggtgaaa
      541 caatttagtc gcatgtttga tgagaataaa actgatcgaa acgatgcatt aagaatcgct
      601 gatttcttgc gtattcaacg attcacaact tcccctatca aagaagagaa atacatggcc
      661 ttacagcgtt taacacgtac tcgctatcaa cttattaagc aattgattcg tacaaagcaa
      721 cattttcttg aaaatctaac gtacaaatgt aatacgcttg ctcgagaaat gcgagacgaa
      781 tcgacgagtc tttttagtgc aaccctcatt tcactcatga cggaagattt cacattggat
      841 gaacttgctg agcttccttt agaagctttc tgtgatttac ttcaagaaaa aggaaaaggc
      901 cgtttcaagc aaccagaaaa aatcgctaaa gcaattcaac gtgcaattac catgagttac
      961 cgcctaggtg ctcttgcaca agaatcaatt aatgttgttt taagtgttct cgtacgagaa
     1021 atacgagcac ttgaaaaaaa tatcaaagaa ttagataaag ccatcgaaca agtaattgtt
     1081 gtcattccag aatatcaatg cttaaccagt attccaggcg ttggtaaagt ctatgctgcc
     1141 ggtttaatcg ctgaaattgg tcaaatcgaa agatttgaag atcagacaaa attggcaaaa
     1201 tacgctggat taagttggaa agtgaaccaa tctggaaact atcagtcgca aaatacacct
     1261 ctgacaaaac aaggaaatcg atattttaga tactacttag ttgaagctgc caactctgta
     1321 aaaaattatc ttcctgaata caaagctttt tatcaaagta aatataagga ggttccaaag
     1381 catcaacaca aacgtgcact cgtcctaacc gcaagaaaat ttacgcgtct ggtggatacg
     1441 ctactacgta aacaccaact ctatacgcca ccaaggagtg tgatagagaa ataacaatcc
     1501 gttattgacg caaatccttg aatcaacccg aaaaaaattt gattttgatc gggtctagtt
     1561 tcgtgcgctt caaaacatat tactcaataa agaaaatctt aaatttcatc t
//
LOCUS       DACEUF010000160         1608 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.167, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000160 DACEUF010000000
VERSION     DACEUF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.167"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(52..507)
                     /gene="tnpA"
                     /locus_tag="IXG20_002631"
     CDS             complement(52..507)
                     /gene="tnpA"
                     /locus_tag="IXG20_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1610766.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            750..1608
                     /locus_tag="IXG20_002632"
                     /pseudo
     CDS             750..1608
                     /locus_tag="IXG20_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          522 a          307 c          305 g          474 t
ORIGIN      
        1 ggggattttt aaaagaccgc caactgctca tcttcactca cttacttctc tttatttttt
       61 aaatggttct acgtactctc ttttacttat aatatatcga atgcgatcat cagcttcttg
      121 ttcacggatg tatttttcat ttgtcttttg attcaatccg actgtactca catagtatct
      181 cttcgaccaa aaggatttgt ttccgtattt atatttgaga tttgcgtgct gctcgtggat
      241 tagtaccgca ctacgacctt tcagataccc cataaaactg gatatacata tttttggtgg
      301 tactcgaaca agcatgtgaa tatgatctag cattgcatgt gcttctatga tttccacatc
      361 tttcatttcg cacaacttcc taagtatttt tccaagatct tttcgtaatt gcccatagat
      421 tacttttcgt tgatattttg gtgtaaaaat aatatgatac ttacaattcc aacgagtata
      481 tgctaaactt ttatcgtcat ttgacatgaa aaaactcctt tcgatctgat gagcggttgg
      541 tagccactca ctagtatatc gaaaggagtt tttatcgcta aagatacttc ttccacgcgt
      601 agaaacgcgc ggttttcttg tttcgcacgc tttacgcaca aaactgactg aagtcattaa
      661 gaaaataaaa gggtctaaaa cttttaagtt ttgtaccgac ccccaaaagt tagaccaaaa
      721 aatctaactt ttggaggtcg gtatttttta tggctaagta tacttttgaa cttaaattaa
      781 agatcgttca tgattacttg gatggtaaag gcggaagtga ttatctggca aagaaatatt
      841 ccattaaggc tccatcacaa gtgaagcgct ggatcaatgc ctatcaagaa tttggtgaag
      901 agggtctagt aagaaagcga caaaagaaaa aatattctgt tcaatttaag ctggatgcga
      961 tagagttata tctaacaagt gagctgtcct atcgtgaagt tgccaacaca cttaacatga
     1021 acaaccctaa tttgatcgct agttggatgc gtcaattccg tgagggtggg atcgatggac
     1081 tctcaaaaac gaaaggatgt ccacctatct tgtctaagaa aaatgaaccc aaaaataaga
     1141 aaaaatctac aactaaagca acgtctaaag aacgtgaacg catcgaggag ttggagaaac
     1201 gagtccgttc ccttcaaatt gaaaatgcct ttttaaaaga attgaggaag ctgcgaaaac
     1261 aggaagccca acaacgacga atgaagcaat cgcacgaatc atcgcaagcc tccgaggacc
     1321 gtttaaatta gttgagcttc tagaaacttt aaagttccct aaggcaatct ttatgtactg
     1381 gcaaaaacga ttggaccgta aaaatccaga tcaagagatt gaagcggaaa tgcttaaagt
     1441 tcgggagaag cacaaggatt atggctgttt acggatgacc aatgaactaa gaaaccgcgg
     1501 tttctctatc aataaaaaga aagttcagcg cctgatcaag aagctaggaa taaaagtaac
     1561 gacatacaca cgtaaatcac ggcgctacaa ttcttatcga ggacaagt
//
LOCUS       DACEUF010000161         1582 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.168, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000161 DACEUF010000000
VERSION     DACEUF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.168"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            292..1479
                     /locus_tag="IXG20_002633"
     CDS             292..1479
                     /locus_tag="IXG20_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1610767.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT          467 a          296 c          315 g          504 t
ORIGIN      
        1 ttttaacctt ccggttttga ttggataacc ctatgaacca tcgttatttt aatttggtat
       61 taactcattt cttttgagat aatatattta ttggattgag aggctaagcc gttcccccct
      121 tgggccattg aacccgcact cgagagactg gctcctctct ctgataagtg gattgcgtat
      181 gagttagatg ttgaaagtcc ttactttact ccgtatctcg tacaaggtag tgacgctttt
      241 cagttgagtg gatttcaatc caaaattctt tttttgaaag gtcagatata catgtacttt
      301 ttaggcattg atatcggtaa acgcactcac gttgcttcta tcatgaacga ggaaggaaag
      361 gtattactaa aaggtttttc atttcctaac accactgaag gagcagagtc attaattgag
      421 cgaatggtcg actattccgg agcacccagt gactttgtta tagggatgga agccacgggg
      481 cactattggc tgtccatctt ttcatacctt cacgaatcag attatttgat tcacgttgtg
      541 aatccccttc aaacagacgg atggcgtaaa ggtacagaaa tacggaaacg aaaaaacgat
      601 attattgatt ctgttttgat tgctgactta atgcgctatg gctcatttgt cgaaacggtc
      661 ttatccgatg aaaatgtttt ttctttgaaa caactttctc gttaccgaac ttatttagta
      721 ggtacagcga gcgacttcaa aagaaaaatt atagccgtat tggatcaagt ctttccagaa
      781 tacgcgacga tttttaccaa gcagggcgtt ttcggaaaag cttctaaaga gttattgctg
      841 gcgtttagtg ccccgatcga cttagaaaac atttcctcag aaactttggc ccaatactta
      901 gctgagttaa gcaggaaccg aatcaaggca gaaaaagcgg accaattgaa gcaagcagct
      961 tctaactcct ttggagtaaa atttgctcaa gaagcgttca ccttccaact acgttcaatg
     1021 attgaacaat taaaattcat tgaaagccaa ataaaggaaa cggaaagaga aatcaagcac
     1081 ataatggaca ctttaaattc tgttattctc acaattcctg gaattggtcc tataaacggt
     1141 gcgactattc ttggtgagat cggagatatt caaaaatttt ctaacccgaa aaaactcgtt
     1201 gcttacgctg ggatagacgc ttccgtgaca caatcagggc aatatgaggc aacgcataat
     1261 gttatgagta aacgagggtc tccatatttg cgaaaagcct tgttttcggc ggctttagta
     1321 gcttctcaat gtgatcctgt ttttagtgcg ttttaccaga aaaaagtttc cgaaggaaaa
     1381 catcatctaa ctgccttagg tgcggtttct cgtaagcttt gttacgtgat tcatgctatt
     1441 ttgactaaaa acgagcctta tataattgtt tctgattagc tattgaacat tcaaaaaaac
     1501 taatggtaag aagcttattt gttgtaccct tttttcggaa ttatctgtta taaaatattt
     1561 tttgatttgg ctaattttta ct
//
LOCUS       DACEUF010000162         1507 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.169, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000162 DACEUF010000000
VERSION     DACEUF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.169"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(606..962)
                     /locus_tag="IXG20_002634"
     CDS             complement(606..962)
                     /locus_tag="IXG20_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ1610768.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /locus_tag="IXG20_002635"
     CDS             complement(976..1386)
                     /locus_tag="IXG20_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1610769.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          464 a          274 c          216 g          553 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgaga
//
LOCUS       DACEUF010000163         1473 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.170, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000163 DACEUF010000000
VERSION     DACEUF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.170"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(41..125)
                     /locus_tag="IXG20_002636"
     tRNA            complement(41..125)
                     /locus_tag="IXG20_002636"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89..91),aa:Leu,seq:caa)
     gene            complement(143..214)
                     /locus_tag="IXG20_002637"
     tRNA            complement(143..214)
                     /locus_tag="IXG20_002637"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(180..182),aa:Gln,seq:ttg)
     gene            complement(224..296)
                     /locus_tag="IXG20_002638"
     tRNA            complement(224..296)
                     /locus_tag="IXG20_002638"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(261..263),aa:His,seq:gtg)
     gene            complement(358..442)
                     /locus_tag="IXG20_002639"
     tRNA            complement(358..442)
                     /locus_tag="IXG20_002639"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Leu,seq:caa)
     gene            complement(463..533)
                     /locus_tag="IXG20_002640"
     tRNA            complement(463..533)
                     /locus_tag="IXG20_002640"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(499..501),aa:Cys,seq:gca)
     gene            complement(579..650)
                     /locus_tag="IXG20_002641"
     tRNA            complement(579..650)
                     /locus_tag="IXG20_002641"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(616..618),aa:Gln,seq:ttg)
     gene            complement(662..734)
                     /locus_tag="IXG20_002642"
     tRNA            complement(662..734)
                     /locus_tag="IXG20_002642"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(699..701),aa:His,seq:gtg)
     gene            complement(737..810)
                     /locus_tag="IXG20_002643"
     tRNA            complement(737..810)
                     /locus_tag="IXG20_002643"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(776..778),aa:Trp,seq:cca)
     gene            complement(817..897)
                     /locus_tag="IXG20_002644"
     tRNA            complement(817..897)
                     /locus_tag="IXG20_002644"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(861..863),aa:Tyr,seq:gta)
     gene            complement(899..974)
                     /locus_tag="IXG20_002645"
     tRNA            complement(899..974)
                     /locus_tag="IXG20_002645"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(939..941),aa:Phe,seq:gaa)
     gene            complement(1010..1082)
                     /locus_tag="IXG20_002646"
     tRNA            complement(1010..1082)
                     /locus_tag="IXG20_002646"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1047..1049),aa:Asp,seq:gtc)
     gene            complement(1092..1164)
                     /locus_tag="IXG20_002647"
     tRNA            complement(1092..1164)
                     /locus_tag="IXG20_002647"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1129..1131),aa:Val,seq:tac)
     gene            complement(1180..1251)
                     /locus_tag="IXG20_002648"
     tRNA            complement(1180..1251)
                     /locus_tag="IXG20_002648"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1216..1218),aa:Glu,seq:ttc)
     gene            complement(1266..1355)
                     /locus_tag="IXG20_002649"
     tRNA            complement(1266..1355)
                     /locus_tag="IXG20_002649"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1319..1321),aa:Ser,seq:gga)
     gene            complement(1395..1468)
                     /locus_tag="IXG20_002650"
     tRNA            complement(1395..1468)
                     /locus_tag="IXG20_002650"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1432..1434),aa:Asn,seq:gtt)
BASE COUNT          419 a          388 c          336 g          330 t
ORIGIN      
        1 atagcaactc tctataagaa agttgctata ttgataatta taccggcggc cggggtcgaa
       61 ccggcacgtc ctcaacggac actggatttt gagtccagcg cgtctgccaa ttccgccacg
      121 ccggcaaatt taaaaataat aactggggta gctggattcg aaccaacgca tgagggagtc
      181 aaagtccctt gccttaccgc ttggctatac cccaagaata aagggcgaat gatgggaatc
      241 gaacccacga atgccggagc cacaatccgg tgcgttaacc acttcgccac attcgccatc
      301 ataaaagatg ttatttaatt tctcaataaa aaactaagat gaagacatct tagtcaatac
      361 cggcggccgg ggtcgaaccg gcacgccctc aacgggcact ggattttgag tccagcgcgt
      421 ctgccaattc cgccacgccg gcaagttgat ttattagtac taaggcggta accggatttg
      481 aaccggtgat gaaggttttg cagacctctg ccttaccact tggctatacc gccattatac
      541 tattatcaat ctataaaata aagaataaat tatagaaact ggggtagctg gattcgaacc
      601 aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca agaataaaaa
      661 gggcgaatga tgggaatcga acccacgaat gccggagcca caatccggtg cgttaaccac
      721 ttcgccacat tcgccatggc aggggcagca ggaattgaac ccacactaac ggttttggag
      781 accgctgttc tacctttaaa ctatgcccct ataaaatgga ggagagtgga ttcgaaccac
      841 tgaaccctaa ggaacggatt tacagtccgt cgcgtttagc cacttcgcta ctcctccatg
      901 gtggcgcggg acagaatcga actgccgaca cacggagctt caatccgttg ctctaccaac
      961 tgagctaccg cgccatattt taaaattatt aattgaataa caagaacgac ggttccgacg
     1021 ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta caccacggaa
     1081 ccagttcatt atggaggtta acgggatcga accgctgacc ctctgcttgt aaggcagatg
     1141 ctctcccagc tgagctaaac ctccaacatt caattaaaat gacccgtacg ggattcgaac
     1201 ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg accaacgggc caggtaaatt
     1261 tgctacggag agtaagggat tcgaaccctt gagacagttt ggcaccatct acatgatttc
     1321 caatcatgct ccttcggcct ctcggacaac tctccagagt gaaagaagct caatactctt
     1381 tctcaaaatg agaactccgg cagtaggact cgaacctacg acatcatgat taacagtcat
     1441 gcgctactac caactgagct atgccggaat aac
//
LOCUS       DACEUF010000164         1464 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.171, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000164 DACEUF010000000
VERSION     DACEUF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.171"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(106..1125)
                     /locus_tag="IXG20_002651"
     CDS             complement(106..1125)
                     /locus_tag="IXG20_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1610770.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1136..1342)
                     /locus_tag="IXG20_002652"
     CDS             complement(1136..1342)
                     /locus_tag="IXG20_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1610771.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          405 a          305 c          246 g          508 t
ORIGIN      
        1 ctattatggg tgatagtgaa gatatgcgat aacatcacta ttttatcgaa tgatttttat
       61 agttgtcaat atagttatgt aatgctcctc aacgaggagc tatttttatc gtttaggaat
      121 atttaaatac caacgtttgt catggaaatc ttgcgcaccg cctttagtgt ttccctctgg
      181 atcattcgtt gcccgcatca tgacatagac tttcttatta gggaagttac gcatgttaaa
      241 agatacatga tagccaacat ttccagaagt attataagct tgatttacat ctgatctata
      301 aattccatca gctcttactc gagctaattc tttcccagta ttgtaatcca taatgaagat
      361 atactcgtat ttatagttag caatgtgcca tccagctaca tgcaaatttg cattttcgat
      421 ttctccaaac tgatcaatgt gggcgtaatt cgttccatct gtcagtgtag gattagctgc
      481 accagctcgt gttggatcaa tgacaggctt gttttcagaa gttgttggat tttcatcggt
      541 aaatccatga gctaaatcat atgctaattt ttctttactt acgcccattt cagaaagata
      601 accgtaagga tctgtatgat cgccccagat gttttgtgtt acccataaat gcgatttgat
      661 tcccggttgg ttataaggag tgtccaacgt taatggaata ccatatttca ttgctgaatc
      721 tctagccaat tcaacgtatg ccttgtagtt tttctcaaac gttgctttat catgtgtgtg
      781 ttgtaactca atctgcacag gactgttggc attagcatac gaaccagcac cgtactgtac
      841 ataaccaggt tgaccgactt gataaacaat tccgccgtct cccacaatat aagcagtata
      901 agcgctagtc catgaacgtt gcatatactg cgcttcattg cgtcctgttg ctgtttcatt
      961 agccgtttca tgcagtaaaa tatactgatt atttgctact tgtgagctac cttcatttgc
     1021 gcccaaatta aattcattgt tgatagtata ggcaaatccg ttaattggca ataaaaaaag
     1081 agccgttaac aggctcatcg cagtaatagt aattttcttt ttcatatcta tcctcctatt
     1141 tatctgatga aattttctta tacgttcgat ttgatacacc taaaatactc cctagaaatg
     1201 ctccaaaacc tgtaataatc atcaccgtta tatctgtgta ccgccagtta acaccttttc
     1261 cgacaagacc gacaaaagtc gctaaagcgg ggataactac tatagcgatc catttcaaaa
     1321 tatcaaaggt tctattgttc atactatcca cctcccttca ttggttgggc tactttcttt
     1381 ttcaatcgct tgttttcttc tatcaatatt tctctttctg aaagcaaggc atcaatttgc
     1441 ccgtccagtt gttcaatttt atct
//
LOCUS       DACEUF010000165         1455 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.172, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000165 DACEUF010000000
VERSION     DACEUF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.172"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            108..1040
                     /locus_tag="IXG20_002653"
     CDS             108..1040
                     /locus_tag="IXG20_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1610772.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1455
                     /locus_tag="IXG20_002654"
     CDS             1112..>1455
                     /locus_tag="IXG20_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610773.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQ"
BASE COUNT          640 a          169 c          227 g          419 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caaga
//
LOCUS       DACEUF010000166         1362 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.173, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000166 DACEUF010000000
VERSION     DACEUF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.173"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            82..>1362
                     /locus_tag="IXG20_002655"
     CDS             82..>1362
                     /locus_tag="IXG20_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ1610774.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DACEUF010000167         1355 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.174, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000167 DACEUF010000000
VERSION     DACEUF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.174"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..>1355
                     /locus_tag="IXG20_002656"
     rRNA            <1..>1355
                     /locus_tag="IXG20_002656"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACEUF010000168         1348 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.175, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000168 DACEUF010000000
VERSION     DACEUF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.175"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..330
                     /locus_tag="IXG20_002657"
     CDS             <1..330
                     /locus_tag="IXG20_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1610775.1"
                     /translation="DFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLTNIENKGQQP
                     SFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVTIGKVIQAIY
                     GEKCKFN"
     gene            complement(844..1011)
                     /locus_tag="IXG20_002658"
     CDS             complement(844..1011)
                     /locus_tag="IXG20_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1610776.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
     gene            complement(1099..>1348)
                     /locus_tag="IXG20_002659"
     CDS             complement(1099..>1348)
                     /locus_tag="IXG20_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HAQ1610777.1"
                     /translation="KYCANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVC
                     LTELKGYQVTNIKSAFQPRRPNNRIDKVRHMFNKINLH"
BASE COUNT          440 a          204 c          227 g          477 t
ORIGIN      
        1 gatttcaaac cattgggtct tgcgattaaa gaagcccgtc ttagtaaagg attgactcgt
       61 gagcaggtag gatcaatgat ccagattgcg ccacgctatt tgacgaacat tgaaaataaa
      121 ggacaacagc caagttttca tgttgtatat gatttagtga ctttgctaaa tatttctttg
      181 gatggattta ttttagggga agaaaacagt cataaaagct ccaaacgtct tcaagtagaa
      241 gcacagttgg attgtctgga tgaagatgaa ttagtgacga ttgggaaagt gattcaagca
      301 atttacggag aaaaatgtaa atttaactag ttcaataata cacaatggat taattaatac
      361 tatcaggaaa gattgattac aaagatgtaa tgaatttttt ctgatttttg ttaatcataa
      421 atagaaaatg aatttatctt tgttacactt aggaactaga gcgaatgata aatagttctt
      481 tggttactct tatcgctgca aagtacagtt cattttttgt tgttgggaaa attttctgga
      541 tttattgcat atcacttcat ccgactaaaa atttttgatt cccatagcga agttcgtttt
      601 tgttccttag gaaaaacttg actgatatac tagttttgaa atgacgagta actctgtact
      661 tcatcaataa tcaagtattg aattttaatt gtatactttt tatgcttgag ggatgtctac
      721 tgtaatacag ctactgcaaa aaaaatcagt acacttattt tcttataaag gatatcaaga
      781 actaagctca atggtatgca tagaaaagaa aaagccgata aaatgagatt ttgatatctc
      841 gaattatcgg ctctgcgtct ttgcgtctgg ctctgtaatt acagttattc taaacttcgt
      901 tatttcaatt aaaatttctt gtctgcattt aggacaaaat agagggaaat tttttaattc
      961 agtatcttcc ctcattttta aacgggtctt attgccacat atagggcaca gtatccacct
     1021 agtatttaca atatctatat cctcctgttc taattaacaa ctcaatattc attttaaaaa
     1081 tttctcttta gttcactatc aatgtaagtt gattttatta aacatatgcc ttactttgtc
     1141 tattctatta tttggacgac gtggttggaa agcagactta atgttagtaa cctgataacc
     1201 ttttaactct gttaaacaga cactgcgtcc atttgtaaag aaagttaaac tgtttcgata
     1261 ctcttgaata caacgggctg gaatttcacc aatgagaatg acctcgttac cttttaactt
     1321 agtatttaaa atatttgcac aatatttg
//
LOCUS       DACEUF010000169         1281 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.176, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000169 DACEUF010000000
VERSION     DACEUF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.176"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            55..315
                     /locus_tag="IXG20_002660"
     CDS             55..315
                     /locus_tag="IXG20_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610778.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
     gene            complement(589..>1281)
                     /gene="tnpB"
                     /locus_tag="IXG20_002661"
     CDS             complement(589..>1281)
                     /gene="tnpB"
                     /locus_tag="IXG20_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1610779.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEK
                     VMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYK
                     ADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          405 a          206 c          194 g          476 t
ORIGIN      
        1 actctcaatt attttaatat aagtacttca gatttaataa aggagaaata tctaatgaaa
       61 aaaattgatt taataaatat gataggtatg ttaataggga tattagtaaa tatagtaatt
      121 ttcacagatt ggctcggggt gttattttct aatcttattc ccatacttat tataggcatt
      181 tgtggaatta tactttcaat attagaactt tttgaaagca gaaatacaat gaatagaata
      241 tttgcttgta ttatattaat tgttaatcta ttgcctatgg tctattttac ttttttatat
      301 tttgcactgg gatgaaataa attaaccaaa agtttctttt tcttataatg tgtatcaata
      361 ttaaaactga aaaagatatg gataatagga tcaaagccct cattggaaca ctcaatatat
      421 tatcagatgt gaactactca ccacttagct aaacctgacg gttcttaacg cttgaagtgg
      481 gagcttcttg ggaatagagc atacttgata gcgtatttct ttactaacag cggtgtctct
      541 tatttaccaa gctatccccg tagttcctac ggttcttgat gattggatct aagccaatcc
      601 catagaatgt agtcttagac cttcggtcag aatattgata ctagcattga tatcacgatc
      661 atgatgagta tgacaaacag gacaagtcca ttctcgaatt tcaagagatt tcttgccatc
      721 tttatgtccg cactctgaac aaatctgact agacggaaac catttatcta ctttgatgat
      781 ttttcgtcca taccagtcag ccttgtattg taatttagtc acaaactttg accaagaaac
      841 atcagaaata cttttagcta atttatgatt tcgcaacata ccttttgtgt ttaagtcttc
      901 aatacagata atatcgtgat ttttgatcat ttctgtactc aacttattca gaaaatcagt
      961 acgttgattc attacttttt catgcaatct agctactttg cgtttttgtt tttgataatt
     1021 tttagcttca aatagattgg tacctttatt tttagccaat aaagcacgcc tagacaattt
     1081 gcgttgttca cgctttagtt tcttttccat tttggacgtg aatttattat tatcaatttt
     1141 ttgtccatca gaaagaatcg caaagtccat aatacctaag tcaataccaa ttgtggaatt
     1201 agttttaggt aattcgataa tttcttcttt acacaacaaa gaaatatagt atttaccgct
     1261 agaatgacgt gatattgtag c
//
LOCUS       DACEUF010000170         1263 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.177, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000170 DACEUF010000000
VERSION     DACEUF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.177"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            849..1061
                     /locus_tag="IXG20_002662"
     CDS             849..1061
                     /locus_tag="IXG20_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610780.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          470 a          189 c          209 g          395 t
ORIGIN      
        1 taaatattat acagataata acggaatatt atcatcgaaa cagctaacaa aattgtcata
       61 aaaaacgaga tggttgatat tagaacttgt gtttttgttt aggtctagag ataaagagag
      121 acaatttcca aatatatgta atgaatacaa aaagacgttt gtcgcggatt accgttacaa
      181 acgtctttta ttgattttcc gagatctcat cagtcgccat ttggaatggt tcactcctta
      241 tctagattcg tttaatgctc ggactgcttg ttcgattgta ataccaaacg caacgttatt
      301 ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt gtatcaaaag ctacgcggta
      361 ttgcatattt gtttttgtaa acgtcataaa attcatctcc tttgtaatgt aatttagttc
      421 ttgaaagttt cctttaaact ttcgaactta taccaagttt agcatcgtac gttttttgat
      481 gcaattaaaa agcatttatc attttcctta tttatttttt cttaaatggt ttagctattt
      541 tcttttaatc cgataagtag cccaatcatc aaggttacaa atactattgc ttgaatataa
      601 atcatatgct cacctcttta taaataactt agcaatataa agaaaaaaac gcacgaacaa
      661 cataggaata taaacaaacg tttttgtcac aacgtctaag atcgtgttaa aaaatagtta
      721 tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa atggataaaa acgttttcaa
      781 atacaaggta atttgttatt ataaaataaa gaaaaaacaa aataaaaata aagaaaagag
      841 ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat atgcaaaccg atcggagtat
      901 ctcatgaact aatcggctgt attgaacgta catatatcaa cacggctgtc gtgtatgttg
      961 aaagttatga ccaacgagac tatcaactta tcaatgaaag caagtatcgc atgctcgtaa
     1021 aattctgtga tattagcgca cctgctgaat tggtttctta aacagtattt ttgagaattc
     1081 gcaagaactc ccttataata taaataaaga cagaagggag cgagaagata tgccaaaaag
     1141 aaaaagttca gggaaaatgg gaattattct tgcagtgatc tctgcaattc ctgctatttt
     1201 agctattgtt cgtgcgatga aggaatcaag agaaagttaa gcacacatta tgagcgtcaa
     1261 aca
//
LOCUS       DACEUF010000171         1247 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.178, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000171 DACEUF010000000
VERSION     DACEUF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.178"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            200..742
                     /locus_tag="IXG20_002663"
     CDS             200..742
                     /locus_tag="IXG20_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610781.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1247
                     /locus_tag="IXG20_002664"
     CDS             832..>1247
                     /locus_tag="IXG20_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ1610782.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          515 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccaga
//
LOCUS       DACEUF010000172         1215 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.180, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000172 DACEUF010000000
VERSION     DACEUF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.180"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            414..947
                     /locus_tag="IXG20_002665"
     CDS             414..947
                     /locus_tag="IXG20_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ1610783.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACEUF010000173         1138 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.181, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000173 DACEUF010000000
VERSION     DACEUF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.181"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(<1..118)
                     /locus_tag="IXG20_002666"
     CDS             complement(<1..118)
                     /locus_tag="IXG20_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HAQ1610784.1"
                     /translation="MSRFFKFGKLHVTKGNGDKLLDILLTASKKLKRSLAPTG"
     gene            complement(123..860)
                     /gene="erm(B)"
                     /locus_tag="IXG20_002667"
     CDS             complement(123..860)
                     /gene="erm(B)"
                     /locus_tag="IXG20_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1610785.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(985..1068)
                     /locus_tag="IXG20_002668"
     CDS             complement(985..1068)
                     /locus_tag="IXG20_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1610786.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT          343 a          185 c          184 g          426 t
ORIGIN      
        1 tccccgtagg cgctagggac ctctttagct tcttggaagc tgtcagtagt atatctaata
       61 atttatctcc attcccttta gtaacgtgta actttccaaa tttaaaaaag cgactcatag
      121 aattatttcc tcccgttaaa taatagataa ctattaaaaa tagacaatac ttgctcataa
      181 gtaacggtac ttaaattgtt tactttggcg tgtttcattg cttgatgaaa ctgattttta
      241 gtaaacagtt gacgatattc tcgattgacc cattttgaaa caaagtacgt atatagcttc
      301 caatatttat ctggaacatc tgtggtatgg cgggtaagtt ttattaagac actgtttact
      361 tttggtttag gatgaaagca ttccgctggc agcttaagca attgctgaat cgagacttga
      421 gtgtgcaaga gcaaccctag tgttcggtga atatccaagg tacgcttgta gaatccttct
      481 tcaacaatca gatagatgtc agacgcatgg ctttcaaaaa ccactttttt aataatttgt
      541 gtgcttaaat ggtaaggaat actcccaaca attttatacc tctgtttgtt agggaattga
      601 aactgtagaa tatcttggtg aattaaagtg acacgaatgt tcagttttaa tttttctgac
      661 gataagttga atagatgact gtctaattca atagacgtta cctgtttact tattttagcc
      721 agtttcgtcg ttaaatgccc tttacctgtt ccaatttcgt aaacggtatc ggtttctttt
      781 aaattcaatt gttttattat ttggttgagt actttttcac tcgttaaaaa gttttgagaa
      841 tattttatat ttttgttcat gtaatcactc ctgaagttat tacatctgta aataaataca
      901 gaagttaaac gatttgtttg taattttagt tatctgttta aaaagtcata agattagtca
      961 ctggtaggaa ttaatctaac gtatttattt atctgcgtaa tcactgtttt tagtctgttt
     1021 caaaacagta gatgttttat ctacattacg catttggaat accaacatga cgaatccctc
     1081 cttcttaatt acaaattttt agcatctaat ttaacttcaa ttcctattat acacaaaa
//
LOCUS       DACEUF010000174         1123 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.182, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000174 DACEUF010000000
VERSION     DACEUF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.182"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(206..760)
                     /locus_tag="IXG20_002669"
     CDS             complement(206..760)
                     /locus_tag="IXG20_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1610787.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          362 a          217 c          130 g          414 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatacttcc gaaaattcag ttatcttcat taatctcacc ttctaagttt tcattataca
      181 caaaaaatat atcctaatat ctcaattagc ctcccaattt aaaaccacgt aacgatgccc
      241 ctatttcatc ttcagttata ccaatgtacc tttttgtaat tgcttgcgag ctatgattaa
      301 aaataaacat taaggtagca atgtctctag ttttcttgta gtagtgatac ccaaaagttt
      361 ttcttaatgt atgtgttcct atatcatctc ttcctaattt ttctgctaca tctgcatata
      421 tctgatagac acgatccctt gttatatgtc gaccttgttg ccgacttgga aataaccaat
      481 cattttcttc tttaccttca gtatataata aaatctcttc tctcaaatta tctataaaaa
      541 tcggttttcg cttacctgtt ttatgttcaa caataatagg cttactttta cctcgtatgt
      601 ctccaacttt taatcctata atatctgaca tacgtaaccc attattgatt ccaaacaaaa
      661 ataaaaaact atctctttct ggattacttg ttcttcttag ccaaaactga aaatcttgta
      721 tctcttcatt tgtccttaac ggttgtacat tatagctcac tattgtttcc cttcttaaaa
      781 atattctgag ttcatgatta tatatatatt ttaccataat catgaactta tatatatact
      841 ctgttaaaag tgaagaaaat caaggggttt agtttatgat tttatatcat ttcagtaact
      901 cagaatattc cgactttttt gtacaaaaaa attaacctta cttatatgaa gattctaagc
      961 tcttatttaa attttagacg ggcagttatc cgtagaagtt cttagaatag ctaatttaca
     1021 taattaaatt atatgaaatt taagtttgaa caatatacta agaactcccc ttactataaa
     1081 gtgaaataaa ttagcatatg aataaccgtg ctaccaaact ttt
//
LOCUS       DACEUF010000175         1082 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.183, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000175 DACEUF010000000
VERSION     DACEUF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.183"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            700..>1082
                     /locus_tag="IXG20_002670"
     CDS             700..>1082
                     /locus_tag="IXG20_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1610788.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMW"
BASE COUNT          413 a          161 c          244 g          264 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 ga
//
LOCUS       DACEUF010000176         1071 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.184, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000176 DACEUF010000000
VERSION     DACEUF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.184"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..90
                     /locus_tag="IXG20_002671"
     CDS             <1..90
                     /locus_tag="IXG20_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1610789.1"
                     /translation="RRNKFYQSLRTASSTIKGMETIRGIYKKN"
BASE COUNT          400 a          142 c          156 g          373 t
ORIGIN      
        1 cgacgaaata aattttatca aagtctccgt acagcctctt ccacgattaa gggcatggag
       61 accattcgag gaatatataa aaagaactga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaaa tcaaggtatt aatgggaata acagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga acccattagc aatatataaa agaaggtatc
      241 aagagtcaca gtgaatttat tccgaaaagt gaaaagagca aatataaaat ttaataaaaa
      301 taagaactaa ataggaaaac gtagacgtaa ttttctacgt tttccttttc atatatatta
      361 aatattacgg aaaattatat aaactaattt agtgttttgt ttgtaattcc taattttgct
      421 ccacttttag ataattttta ggtctattta actcattaga cattctatgc tcatttttct
      481 ccaaatttgt ttcaactctt tcgtacttaa ttttgcgtat ttgaaaattc ttcttttaga
      541 gtacttttat tacgatacta gtatgatata tatctacagc aaacagttaa aatcttccta
      601 aaataagcga atagcattcc taaaacctat aaaattttta gatgaagcga caagtggaca
      661 attaataaac tacttgaaca tgaaattatg gatcaccttt caaatttaga aaatggaatt
      721 cttatactta ttgcgcaaca tttattgatt gcaaaattat gtgacaaaaa gaactagtag
      781 aagaaggaat aatggcttga taataaagta ctacctatta aaggtagtac tttatttaat
      841 ttagtataaa ttaacatact actatatttt aggtttcata ttatataaat taataatata
      901 ctaccatttg ttggtagtat aatttaaagg taataagtta ttcttttatt actatgtttg
      961 ggttttatag caaagaaagt ttaataagag tttggaccta gcaaacaata ttaatgaggt
     1021 cattaaatct tattatcttt agtgatataa tgaaatatct acacgacaaa a
//
LOCUS       DACEUF010000177         1035 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.185, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000177 DACEUF010000000
VERSION     DACEUF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.185"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(<1..>1035)
                     /locus_tag="IXG20_002672"
     rRNA            complement(<1..>1035)
                     /locus_tag="IXG20_002672"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACEUF010000178         1005 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.186, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000178 DACEUF010000000
VERSION     DACEUF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.186"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(132..>1005)
                     /gene="menA"
                     /locus_tag="IXG20_002673"
     CDS             complement(132..>1005)
                     /gene="menA"
                     /locus_tag="IXG20_002673"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ1610790.1"
                     /translation="FSIAFFHEIHWLNTFLFFIGMLVFDLATTAINNYMDYEKAHSEV
                     YKYEENVIGRRNLSPVLIRNMILAMIAFVFVIGVILTINTGWLLLLMGFICCFIGVFY
                     TFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVNTFGSDVYDLTVNFSSGQFDLVGNLW
                     AIAAIVLASLPLVFTIANIMLANNLRDLDRDIENHRYTLVFYIGRPAGTVLFQLLMYA
                     CYFVILMGLLSGIYEWPILVTFLTLPIVKKHLDQFKQTLPQPISFSYSIKNMVLFNAS
                     YVLGLLLTIIMQAI"
BASE COUNT          369 a          198 c          175 g          263 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaat
//
LOCUS       DACEUF010000179          974 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.187, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000179 DACEUF010000000
VERSION     DACEUF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.187"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            6..>974
                     /locus_tag="IXG20_002674"
     CDS             6..>974
                     /locus_tag="IXG20_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1610791.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYI"
BASE COUNT          345 a          127 c          171 g          331 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tata
//
LOCUS       DACEUF010000180          942 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.188, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000180 DACEUF010000000
VERSION     DACEUF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.188"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            210..911
                     /locus_tag="IXG20_002675"
     CDS             210..911
                     /locus_tag="IXG20_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1610792.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          337 a          163 c          151 g          291 t
ORIGIN      
        1 cgtttactat atgtattatt gttgtttttt atacacaaat cattcgcttc aaaacaaatt
       61 attcaatacc attgtatatt ttttacataa taacaaatta tatttgtaaa gtattaaaac
      121 ttgtttctaa tgttggtatg ttaaaacttt acaaattctt taaaaaaagt gacaataaat
      181 atagtgaaca ataaggagag gactttttaa tgcgtgttaa taaaatcgat tttcactttt
      241 caaaactcag atatcaacct gattttgaac atttctcgga tgaagagttc caacagatca
      301 aggaacatac tgttcttcgt tcttataaaa aaggacagat ccttttcgac gatggtgatc
      361 ccagacaacg gttgtatatc ttgaccaaag gactcgttcg tttagaacgt tatgatgaaa
      421 gtgcaactta ttactactat gactatgtga atacaaataa cctttttcct atgtcaggaa
      481 tgtttggaga agagacctac acttatacag ctcaagcgat gactgatatt gaaacattct
      541 accttcctgt ttcctactat gaaaaattag cacgaaaaaa tcccgaacag ttgatctata
      601 tgatacaaaa aatttctaaa gtagttgaaa tacatgaatt gcgtatccaa atcggattga
      661 cctctagcgc ctttgaccgg gtaaaacaaa atttattcat tttgaaagaa gaattgggcg
      721 taccaacaaa agatggtcat atcaccattc cttatccgat caccttaaaa gaattagcag
      781 tgaatagcgg cacgacacgt gaaactgctg gacaagtaat caaacaacta cgtgattccg
      841 ggctgttaga ttatgacaaa aagcgtttta ctttctgtaa gacagctact actccagcta
      901 tgagagaata acgcgaacaa aaataagcag tgaccctgat aa
//
LOCUS       DACEUF010000181          895 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.189, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000181 DACEUF010000000
VERSION     DACEUF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.189"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..618
                     /locus_tag="IXG20_002676"
     CDS             <1..618
                     /locus_tag="IXG20_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1610793.1"
                     /translation="PFYKEEESLMPYFALAAQRAGAVGIRANSVRDIIEIRKCVSLPM
                     IGIIKRDYPPESPFITATMREVDELVETGVEVIALDCTLRNRHDGLSINSFITKIKEK
                     YPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGYTEESKKQDGPDIDLIKKLVEEGIC
                     VIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQEIASRFVKALS"
     gene            632..>895
                     /locus_tag="IXG20_002677"
     CDS             632..>895
                     /locus_tag="IXG20_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1610794.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAG"
BASE COUNT          313 a          116 c          194 g          272 t
ORIGIN      
        1 ccattttaca aagaggaaga aagtttaatg ccttattttg ctttggctgc tcaaagggct
       61 ggagcagttg gtatacgtgc aaattctgtt cgagatatta ttgaaattag aaagtgtgta
      121 tcattaccaa tgattggaat tattaaaaga gattatccac cagaatctcc attcattact
      181 gccactatga gagaagtaga tgaattagta gaaaccggtg ttgaggtaat cgctcttgat
      241 tgcactctta gaaatcgaca tgatggacta tctataaatt cttttattac taaaataaaa
      301 gaaaaatatc ctcatcagtt atttatggca gatatttcta catttgaaga aggcaaaaat
      361 gcatataaag caggaataga ttttgtagga actactttaa gtggatatac tgaggaaagt
      421 aaaaaacaag atgggccaga tatagatttg attaaaaaat tggtagaaga agggatttgt
      481 gtaattgctg agggaaggat tcatactcca gaacaagcaa aagagataaa aaaaatagga
      541 gttgcaggta ttgttgtggg tggtgctatt actcgaccac aggaaattgc atcaagattt
      601 gttaaagcat taagttaggg ggagaaataa aatgtttaaa aaattttcac aattaggacg
      661 cgctttcatg ttaccaattg ctatattacc agtagccgga ttactattgg gactgggagg
      721 agcgttaaca aatgagagtg ctataaatgc ttatccattt ttagatcaac cgtggttgca
      781 tacaatttta agtattatga gttatgctgg aaatgcagtt tttgcaaacc tagcactaat
      841 ttttgcaatt ggtattgctg taggacttgc aaatggagat aaaggaacag ctggt
//
LOCUS       DACEUF010000182          890 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.190, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000182 DACEUF010000000
VERSION     DACEUF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.190"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..888
                     /locus_tag="IXG20_002678"
     CDS             <1..888
                     /locus_tag="IXG20_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ1610795.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACEUF010000183          862 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.191, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000183 DACEUF010000000
VERSION     DACEUF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.191"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(<1..434)
                     /locus_tag="IXG20_002679"
     CDS             complement(<1..434)
                     /locus_tag="IXG20_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1610796.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /locus_tag="IXG20_002680"
     CDS             complement(451..855)
                     /locus_tag="IXG20_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ1610797.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          260 a          139 c          136 g          327 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat at
//
LOCUS       DACEUF010000184          860 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.192, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000184 DACEUF010000000
VERSION     DACEUF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.192"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..856
                     /locus_tag="IXG20_002681"
     CDS             <1..856
                     /locus_tag="IXG20_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1610798.1"
                     /translation="KRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHA
                     LLDIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFH
                     IVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRA
                     SFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPET
                     YTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFKLRIMIR
                     HGKTFLTK"
BASE COUNT          273 a          173 c          165 g          249 t
ORIGIN      
        1 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
       61 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      121 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      181 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      241 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      301 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      361 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      421 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      481 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      541 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      601 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      661 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      721 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      781 gtacggcttt cgcaactttt acaatttcaa actacgaatt atgattcgtc atggcaaaac
      841 atttttaacc aaataaacag
//
LOCUS       DACEUF010000185          826 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.193, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000185 DACEUF010000000
VERSION     DACEUF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.193"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            192..290
                     /locus_tag="IXG20_002682"
                     /pseudo
     CDS             192..290
                     /locus_tag="IXG20_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(513..743)
                     /locus_tag="IXG20_002683"
     CDS             complement(513..743)
                     /locus_tag="IXG20_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ1610799.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
BASE COUNT          299 a          127 c          136 g          264 t
ORIGIN      
        1 accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt tactgataga
       61 cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat tttggcaatt
      121 tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat tcaaacatta
      181 gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga acacctttgg
      241 aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga caattaagag
      301 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
      361 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      421 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      481 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      541 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      601 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      661 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      721 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      781 tttcaagttg atcaatagct tccacgataa aaatatcttt agataa
//
LOCUS       DACEUF010000186          791 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.194, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000186 DACEUF010000000
VERSION     DACEUF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.194"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..782
                     /locus_tag="IXG20_002684"
     CDS             <1..782
                     /locus_tag="IXG20_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1610800.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          242 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc t
//
LOCUS       DACEUF010000187          751 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.195, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000187 DACEUF010000000
VERSION     DACEUF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.195"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            36..>751
                     /locus_tag="IXG20_002685"
     CDS             36..>751
                     /locus_tag="IXG20_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1610801.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLY"
BASE COUNT          280 a           96 c          123 g          252 t
ORIGIN      
        1 taatggagaa aagttgaaat tgtagaggag tttgtatgag ttatatctat ttaattaaag
       61 aaaattatcc atttttaacg aagtctgaaa aaaaagtcgc tgattttatt ttaaattctg
      121 ggaaaactat tatttatagt acgatgagtg atattaagaa aaacacaaaa gttggcgatg
      181 ctactattat tcgcttttgt caaaaaattg gtttttctgg tttttctgat ttaaaaattg
      241 aaattgcaaa ggaagacttt agtcaaaaaa aagaacatcc gactagtgta aagtactatg
      301 atgaaattgc taatagttta acagaagctc tctattccac tattaggctt ttagacgaag
      361 aaaaattgaa tgaagccatt aaaatgatca ctcaatcaaa aaacatatat atatttggag
      421 tgggatctag tggaaatact agtcttgatt tagaaaatat gtttcttcgc gtaggagttc
      481 aagcaaaagc tgtactagac cctcattttc aagcccaagt cgcttcttta ttaacagtta
      541 atgatttagt aatcattttt tctttatctg ggaaaacgaa agatacttat gattcattga
      601 aaatagctaa aaaaaatggc gcaaaaatta ttgctataac taactatatt cattcaccta
      661 taggaaagtc tgcagactta gttttacaaa cagccattga agaatttctt aatggtggat
      721 ctttagctgg aaaaatatca caactttata t
//
LOCUS       DACEUF010000188          735 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.196, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000188 DACEUF010000000
VERSION     DACEUF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.196"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            27..>735
                     /locus_tag="IXG20_002686"
     CDS             27..>735
                     /locus_tag="IXG20_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1610802.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISN"
BASE COUNT          260 a          127 c          139 g          209 t
ORIGIN      
        1 caaagaatta taaggagtga gtacaaatga cctataccca tcttacaacg gatgaacttg
       61 taataataga gtcttatttc aaaatgaatc aatctgttgc taaaactgcc cattgcttga
      121 atcgttcaag acaaacgatc cataaagtat atctgttctt caagcaagga aaatcagcct
      181 tagaatatta tcaacagtat aagaaaaaca aatcaaactg tggtagacgt ccgcttgttt
      241 tacccgagga acaatcagaa tatattcaaa gaaaggttgt tcaaggatgg acacccgatg
      301 tgattgttgg tcgtgcagcg tttcctatta gttgttctgc tcgtaccatt tatcgtatgt
      361 tcaaaaaggg gctatttgat tcttctgact taccgatgaa aggcaagcgt aaaccgaatg
      421 gacatcaaga aagacgtgga aaacaaactt tccgccgctc tattcatgaa cgtgaaaagg
      481 attatagcca attctcaaat gagtttggtc accttgaagg tgacactatc gtaggtctga
      541 aacataaaag tgctgtaatt accttagttg aacgattatc aaaagttatc atcacattga
      601 aaccgtgtgg tagacaagcg attgatattg aaaaaaaatt aaatcaatgg tttgaatctg
      661 taccgaaaaa cctattcaaa tccatcactt ttgattgtgg aaaggaattt tcaaattgga
      721 aacagatcag taatg
//
LOCUS       DACEUF010000189          727 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.197, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000189 DACEUF010000000
VERSION     DACEUF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.197"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            1..727
                     /locus_tag="IXG20_002687"
                     /pseudo
     CDS             1..727
                     /locus_tag="IXG20_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          272 a          124 c          138 g          193 t
ORIGIN      
        1 tacgtaaaag aaaccagcaa ttagaaatgg aaaatgatat tttaaagcaa gcagcgctga
       61 tattcggacg aagagacaag taatcgatgc gaataagcat ctttacccta tatcagcgat
      121 gtgcagaata ttaggtctat cacgtcagtc ctattattat caatcaaaac caaagaaaga
      181 cgaatcagaa cttgaagaag tagtcgctga agaatttatc cgcagccgaa aggcctacgg
      241 ctcaagaaaa ataaaaaaag ccttatcaaa acgaggcatt cagatcagcc gacgaaaaat
      301 tagtagaatc atgaaaaata gaggattaaa atcgagctat actgttgctt attttaaagt
      361 acatcattct acttgcaatg aagccaaaac gacaaacgta ttgaatcgta aattcttaag
      421 agacaaccca ttagaagcga tcgtaacaga cttgacttat gtacgagtcg ggaaaaaatg
      481 gaattatgtc tgtttcattt tggatctgtt caatcgagaa attctcggct attcttgtgg
      541 agaacataaa gatgccgttc tagtaaaaaa agcatttagc cgtatcaaac aacctctgac
      601 agaggttgag atttttcata ctgatcgtgg aaaagagttt gataaccaag ctattgatga
      661 attattaaca acttttgaca tcaatcgatc attgagtcat aaaggctgtc cttttgataa
      721 tgccgta
//
LOCUS       DACEUF010000190          718 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.198, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000190 DACEUF010000000
VERSION     DACEUF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.198"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..696
                     /locus_tag="IXG20_002688"
     CDS             <1..696
                     /locus_tag="IXG20_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1610803.1"
                     /translation="QLPFLKRYFSHFSREIREAVEWIVIDMYAPYVSLVKKLFPKAQL
                     IIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQKHYQLGKQLKRYWKLLQKDERKLDYT
                     RRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFLYAVKERDYVSFEELLTNNI
                     MLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKL
                     RIMIRHGNALIFN"
BASE COUNT          241 a          146 c          127 g          204 t
ORIGIN      
        1 caactgcctt ttttaaaacg ttattttagt catttttcac gagaaattcg tgaagcggtc
       61 gaatggatcg tgattgatat gtacgctcct tatgtatcgc tcgttaaaaa gctgtttcca
      121 aaagcacagc tgattattga ccggtttcac atcgtccaac atattggtcg tacctttcgc
      181 aatcatcgaa tcaaagaaac gaaccaacta ttaaaaagca aagaacaaaa acactatcaa
      241 ttaggtaagc aattaaaaag atactggaaa cttcttcaaa aagatgaacg caagcttgat
      301 tatactcgac gcctttggcg acctggcttc aaagctcatt taacagagac cgatattgtg
      361 gatcgcttac tgaaagggag ccctgctttg cgtgtaggct atcagttata tcaagacttt
      421 ctttacgctg taaaggaacg agactacgta tcgtttgaag aactacttac caataatatc
      481 atgcttcccg aaggctatca gacgaccttg agaacgttcc agaaatttct gccacaaatc
      541 aaaaatgctt tacaacaaag ttattcaaat ggcccacttg aatgtctgaa caaccatatc
      601 aaagtgttaa aacgaaatgc atatggcttt cgtagctttt ataatttcaa attgcgcatc
      661 atgattcgtc atggcaacgc attgattttc aactaaaaaa gatcaagagg aactgatc
//
LOCUS       DACEUF010000191          685 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.199, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000191 DACEUF010000000
VERSION     DACEUF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.199"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            116..331
                     /locus_tag="IXG20_002689"
     CDS             116..331
                     /locus_tag="IXG20_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ1610804.1"
                     /translation="MKKKLVKGSVICSMIGIGFIVIGTNVEAATYYGNGVYCNKQKCW
                     VNWGQAWSKGVKRWGDNLFGSFSGGRI"
     gene            332..673
                     /locus_tag="IXG20_002690"
     CDS             332..673
                     /locus_tag="IXG20_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1610805.1"
                     /translation="MIDIRETKKIIHELYNSLMKRDKTKAILDITDVLLQVYKKIDSE
                     KYPEILINKLVNYIYIVGFDNKIHFLGNDEKLLIELGDISKKAGINSKYKANFTDKSQ
                     FYSYSEKVPIR"
BASE COUNT          259 a           58 c          132 g          236 t
ORIGIN      
        1 attttgagtg tagcacaaat gactttttta gttgtctagc ttaattttac aatcggcgct
       61 atataatttc tacatagaag acgttttcta atttttattt tcgaggtgaa tattaatgaa
      121 aaagaaatta gttaaaggct cagttatttg tagcatgatt gggattggtt ttatagtaat
      181 aggaacaaat gtagaagctg ccacgtatta cggaaatggt gtgtattgta ataagcaaaa
      241 atgttgggta aattggggac aagcttggtc aaaaggggtt aaacgttggg gagataattt
      301 gtttggtagt tttagtggag gaaggattta aatgattgat attagggaaa caaaaaaaat
      361 aattcatgaa ttgtataata gtttaatgaa gcgtgataag accaaagcta tattagatat
      421 tacagatgtt ttgttgcaag tctataaaaa aatagatagc gaaaaatatc cagaaatatt
      481 aataaacaaa ttggtgaatt atatctatat tgttggattt gacaataaaa ttcatttttt
      541 aggaaatgat gaaaagttat taatagaatt gggagatata tctaaaaagg ctggaattaa
      601 cagtaaatat aaagctaatt ttacagataa atctcaattc tatagttatt ctgaaaaagt
      661 acctattaga taattatatt tccct
//
LOCUS       DACEUF010000192          582 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.200, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000192 DACEUF010000000
VERSION     DACEUF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.200"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            143..400
                     /locus_tag="IXG20_002691"
     CDS             143..400
                     /locus_tag="IXG20_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610806.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          240 a           72 c           80 g          190 t
ORIGIN      
        1 ttttctgtcc acgttcattt tactccaaag ttttcctcat ttcaaaagaa aatcacagaa
       61 tacacgaaga agcgtttcta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaataa
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 actttacatt tttttaggaa tgtaaagtct tatctctttt ct
//
LOCUS       DACEUF010000193          528 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.201, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000193 DACEUF010000000
VERSION     DACEUF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.201"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(5..90)
                     /locus_tag="IXG20_002692"
     tRNA            complement(5..90)
                     /locus_tag="IXG20_002692"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IXG20_002693"
     tRNA            complement(107..178)
                     /locus_tag="IXG20_002693"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IXG20_002694"
     tRNA            complement(190..262)
                     /locus_tag="IXG20_002694"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IXG20_002695"
     tRNA            complement(280..361)
                     /locus_tag="IXG20_002695"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IXG20_002696"
     tRNA            complement(369..441)
                     /locus_tag="IXG20_002696"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IXG20_002697"
     tRNA            complement(445..517)
                     /locus_tag="IXG20_002697"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACEUF010000194          520 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.202, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000194 DACEUF010000000
VERSION     DACEUF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.202"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..>520
                     /locus_tag="IXG20_002698"
     rRNA            <1..>520
                     /locus_tag="IXG20_002698"
                     /product="23S ribosomal RNA"
BASE COUNT          149 a          100 c          158 g          113 t
ORIGIN      
        1 agcccgttaa tgggtgatgg cgtgcctttt gtagaatgaa ccggcgagtt acgattgcat
       61 gcgaggttaa gttgaagaga cggagccgca gcgaaagcga gtctgaatag ggcgtttgag
      121 tatgtagtcg tagacccgaa accatgtgat ctacccatgt ccaggttgaa ggtgcggtaa
      181 aacgcactgg aggaccgaac ccacgtacgt tgaaaagtgc ggggatgagg tgtgggtagc
      241 ggagaaattc caaacgaact tggagatagc tggttctctc cgaaatagct ttagggctag
      301 cctcggaatt gagaatgatg gaggtagagc actgtttgga ctaggggccc atctcgggtt
      361 accgaattca gataaactcc gaatgccatt cattcatatc cgggagtcag actgtgagtg
      421 ataagatcca tagtcgaaag ggaaacagcc cagaccacca gctaaggtcc caaaatatat
      481 gttaagtgga aaaggatgtg gggttgcaca gacaactagg
//
LOCUS       DACEUF010000195          487 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.204, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000195 DACEUF010000000
VERSION     DACEUF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.204"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(<1..>487)
                     /locus_tag="IXG20_002699"
     CDS             complement(<1..>487)
                     /locus_tag="IXG20_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAQ1610807.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DACEUF010000196          466 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.205, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000196 DACEUF010000000
VERSION     DACEUF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.205"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            326..>466
                     /locus_tag="IXG20_002700"
     CDS             326..>466
                     /locus_tag="IXG20_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1610808.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKY"
BASE COUNT          146 a           67 c           84 g          169 t
ORIGIN      
        1 tatttcttta ttttttttta gtagtaattc ttggaatttg gataagatat tgaactttaa
       61 atcatggtat tttacccaag gtttatggga aatggagggt atgtggtttt ggaaagcgtt
      121 catttttgaa actccgtttg ttttactccg tgtattaccg ttatttttct ttctgttttt
      181 gggtatctat tatcttttac gttcacttat aaatgatgat tcaacagtga taacaaaata
      241 gaatattttg tgtaaataga aaaaaggaag tcccttgtgt agaatagagt taccacaaca
      301 cattcacaga aaagaggact tccatatgaa cgattttact acagaaattc taaagactct
      361 agcgaacaaa ggcgatttga atgaattatt ccgtgtccat ttggaaaaag ctgtcaatac
      421 gcttctcaaa acggagttaa cggctttcct cgattacgaa aagtac
//
LOCUS       DACEUF010000197          440 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.206, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000197 DACEUF010000000
VERSION     DACEUF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.206"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            44..>440
                     /locus_tag="IXG20_002701"
     CDS             44..>440
                     /locus_tag="IXG20_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1610809.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEI"
BASE COUNT          137 a           83 c           86 g          134 t
ORIGIN      
        1 cctttagaat taatttggct accaattcat aggaggattc aagatgtctc atactcagct
       61 tatcaaagat accctaaata ttctagacct aaatatctat ttcgaggaaa attgtttaac
      121 taaagaaaaa tacaaagggc aaatttgctt gatttacaaa ggaactcttc tgtacaagcc
      181 agaagagtgt tcccattgtc tttgtgtcgt cccctctagg atcattcgtt gggggacgac
      241 aaccgttcgg ttgttactga atgatgtgtc tgagtatcgc acttatctgg agctgaagaa
      301 acaacgattt aaatgtaagt cttgccaaag aacattcgta gcggatacct ctgttgctga
      361 aaaacactgt ttcattagtc aaaaagttcg ttggtctgtc attgctcgct taaaagagaa
      421 tacttccatg acggaaatcg
//
LOCUS       DACEUF010000198          440 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.207, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000198 DACEUF010000000
VERSION     DACEUF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.207"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            11..>440
                     /locus_tag="IXG20_002702"
     CDS             11..>440
                     /locus_tag="IXG20_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1610810.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          168 a           57 c           88 g          127 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag
//
LOCUS       DACEUF010000199          421 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.208, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000199 DACEUF010000000
VERSION     DACEUF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.208"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..>421
                     /locus_tag="IXG20_002703"
     CDS             <1..>421
                     /locus_tag="IXG20_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1610811.1"
                     /translation="AAFPIRCSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQ
                     AFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQA
                     IDIENKLNHWFESVPKNLFKSITFDCGKEFSNWKQISN"
BASE COUNT          142 a           72 c           81 g          126 t
ORIGIN      
        1 gcagcgtttc ctattcgttg ttctgctcgt accatttatc gtatgttcaa aaaggggcta
       61 tttaatcctt ctgacttacc gatgaaaggc aagcgtaaac caaatggaca tcaagaaaga
      121 cgtggaaaac aagctttccg tcgctctatt catgaacgtg aaaaagatta tagccaattc
      181 tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtttgaaaca taaaagtgct
      241 gtaattacct tagttgagcg attatcaaaa gttatcatca cattgaaacc gtgtggtaga
      301 caagcgattg atattgaaaa caaattaaat cattggtttg aatctgtacc gaaaaatcta
      361 ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca gatcagtaat
      421 g
//
LOCUS       DACEUF010000200          400 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.209, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000200 DACEUF010000000
VERSION     DACEUF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.209"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
BASE COUNT          136 a           59 c           48 g          157 t
ORIGIN      
        1 tttaaaaaat gataaacctc tctcgaacgg ttttttatca aaaatcgtca tttttccatc
       61 aaaatctgct tatgcaaaat tataaaaaaa ttttgcagtt tttcttactt tttttgtcgt
      121 ttaattgaat gataaaaacc agtttcttta ttcagttaat caaatatgaa cgaattagat
      181 atgaatcatt gtatttgccg ttgcggaagg attttttaat taaccacaga taaacattga
      241 taacgattcg cttattgtaa tcaacagatt aaatttcata tgttcatatg tacggataca
      301 ttttcggacc gattaatttt aaccattcta tttatttgct tacctattag atatcaataa
      361 aaaactgctt aacagctatt tcagctgtta agcagtttta
//
LOCUS       DACEUF010000201          380 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.210, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000201 DACEUF010000000
VERSION     DACEUF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.210"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(<1..>380)
                     /locus_tag="IXG20_002704"
     CDS             complement(<1..>380)
                     /locus_tag="IXG20_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1610812.1"
                     /translation="FLIAAQQLEKMGYVNENYLNGLIAREREFPTGLITKYLNIALPH
                     SECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVENLFFLGIKEPKEQVGLLQAFMNLF
                     MDETFVENYKKQETPQNIYELITN"
BASE COUNT          113 a           69 c           53 g          145 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag
//
LOCUS       DACEUF010000202          375 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.211, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000202 DACEUF010000000
VERSION     DACEUF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.211"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..>375
                     /locus_tag="IXG20_002705"
     CDS             <1..>375
                     /locus_tag="IXG20_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1610813.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IAVGIRPDGSKKVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNIVHKV"
BASE COUNT          104 a           85 c           87 g           99 t
ORIGIN      
        1 atgactaaat catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt
       61 tatgccgcta tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag
      121 gaagccattc atatcgcagt tggcattcgc ccggacggtt caaagaaagt gttgagctat
      181 gcgattgcac caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa
      241 cgtggcgtga aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc
      301 gtgcagcggt tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat
      361 attgttcata aagta
//
LOCUS       DACEUF010000203          372 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.212, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000203 DACEUF010000000
VERSION     DACEUF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.212"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            82..>372
                     /locus_tag="IXG20_002706"
     CDS             82..>372
                     /locus_tag="IXG20_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1610814.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRT"
BASE COUNT          132 a           61 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cc
//
LOCUS       DACEUF010000204          348 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.213, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000204 DACEUF010000000
VERSION     DACEUF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.213"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..>348
                     /locus_tag="IXG20_002707"
     CDS             <1..>348
                     /locus_tag="IXG20_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAQ1610815.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCT"
BASE COUNT          119 a           70 c           70 g           89 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactga
//
LOCUS       DACEUF010000205          308 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.214, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000205 DACEUF010000000
VERSION     DACEUF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.214"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            50..>308
                     /locus_tag="IXG20_002708"
     CDS             50..>308
                     /locus_tag="IXG20_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1610816.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKW"
BASE COUNT          120 a           41 c           61 g           86 t
ORIGIN      
        1 actatcaaat aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatcattt
       61 taaaggaaag caatttcagc aggatgtgat tattgtagcc gtgggctact atcttcgtta
      121 taaccttagc tatcgtgaag ttcaagaaat cttatatgat cgtggcatta acgtttctca
      181 tacgacgatt tatcgttggg tgcaagaata tggcaaacta ctctatcaaa tttggaaaaa
      241 gaaaaataaa aaatcctttt attcatggaa aatggatgaa acgtacatca aaattaaagg
      301 aaaatggc
//
LOCUS       DACEUF010000206          296 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.215, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000206 DACEUF010000000
VERSION     DACEUF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.215"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(<1..>296)
                     /locus_tag="IXG20_002709"
     CDS             complement(<1..>296)
                     /locus_tag="IXG20_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1610817.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEK"
BASE COUNT           80 a           60 c           50 g          106 t
ORIGIN      
        1 tccttttcta agtccaaagt aaaagggcgt tttccagcct ctattcgttg acgattgatc
       61 tcatcttcta attcatcctg ataggctttc gcttcatcat gggtcatttc tttcgtaaac
      121 ttatgtttat tggcgttagc tttgatgtgg gtagaatcca gatacaaatt atcctctgtt
      181 acaaaacctg ctttaactgc ttgttctaaa atataggcaa aaatatcctc aaacaccgtc
      241 gtttctctaa atcggcgaac ataattcttt ccaaaagttg agaaatgggg gatttt
//
LOCUS       DACEUF010000207          259 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.216, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000207 DACEUF010000000
VERSION     DACEUF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.216"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..>259
                     /locus_tag="IXG20_002710"
     CDS             <1..>259
                     /locus_tag="IXG20_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1610818.1"
                     /translation="FGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIET
                     ALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFA"
BASE COUNT           81 a           47 c           44 g           87 t
ORIGIN      
        1 gtttggtcac cttgaaggtg atacgatcgt tggcattcat cataaaagtg ccgtcattac
       61 tttagttgaa agattatcta aagtcattat cacgattaaa cccaacggcc gtaaggcatt
      121 agatattgaa actgccctta atcaatggtt ttctcgcttc cctaaaaact tctttaaatc
      181 tattacgttt gactgtggaa aagaattttc taactggaaa gccattagta accaacatga
      241 tattgatata tattttgcg
//
LOCUS       DACEUF010000208          249 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.217, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000208 DACEUF010000000
VERSION     DACEUF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.217"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..>249
                     /locus_tag="IXG20_002711"
     CDS             <1..>249
                     /locus_tag="IXG20_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1610819.1"
                     /translation="SKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIVEA
                     TYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           71 a           50 c           47 g           81 t
ORIGIN      
        1 gtctaaaaac aatacgaatg gtagaaatca atttgcgatg cttacgattg atgatctagt
       61 tcctcaagat catctcgttc gaaagattga tgccgcgctt gattttgaat ttatttatcc
      121 aatcgtcgaa gccacttact ccgatttagg tcgcccaagt atcgatccag tgattctgat
      181 caaattggtg tttattcagt atttattcgg tatccgttcc atgcgacaaa ccattaagga
      241 agtcgatac
//
LOCUS       DACEUF010000209          244 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.218, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000209 DACEUF010000000
VERSION     DACEUF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.218"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(<1..>244)
                     /locus_tag="IXG20_002712"
     rRNA            complement(<1..>244)
                     /locus_tag="IXG20_002712"
                     /product="23S ribosomal RNA"
BASE COUNT           52 a           81 c           49 g           62 t
ORIGIN      
        1 ttggacagac atttccgatc gtctgcattc cttagcctcc tgcgtccctc cattgctcaa
       61 acaaaaacaa cgggtacagg aatatcaacc tgttatccat cgcctacgcc tgtcggcctc
      121 ggcttaggtc ccgactaacc ctgggcggac gagccttccc caggaaacct tagtcattcg
      181 gtggacagga ttctcacctg tctttcgcta ctcataccgg cattctcact tctaagcgct
      241 ccag
//
LOCUS       DACEUF010000210          236 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.219, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000210 DACEUF010000000
VERSION     DACEUF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.219"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            26..>236
                     /locus_tag="IXG20_002713"
     CDS             26..>236
                     /locus_tag="IXG20_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1610820.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRL"
BASE COUNT           86 a           48 c           39 g           63 t
ORIGIN      
        1 aaccaaccca aaaggaggaa tcaagatgtc ttacactcac cttatcaaag aaacattaga
       61 tattctagac ttatcagtta cttttaacga aaattgtctc acaaaagaaa aatacaaagg
      121 gcaaatctgt catatttatc gtggaaactt gatttataca gcccaagaat gcatccactg
      181 taaacaccag attgcttcag atattgtgcg ttggggtaca acaaccgtcc ggttgt
//
LOCUS       DACEUF010000211          227 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.221, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000211 DACEUF010000000
VERSION     DACEUF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.221"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            1..227
                     /locus_tag="IXG20_002714"
                     /pseudo
     CDS             1..227
                     /locus_tag="IXG20_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT           94 a           43 c           44 g           46 t
ORIGIN      
        1 tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct cattcaaaga
       61 aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga aaaataagca
      121 actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac gaaaagacaa
      181 gtaattgatg ccaacaagca taaatattcc atatcagcga tgtgcaa
//
LOCUS       DACEUF010000212          224 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.222, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000212 DACEUF010000000
VERSION     DACEUF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.222"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..>224
                     /locus_tag="IXG20_002715"
     CDS             <1..>224
                     /locus_tag="IXG20_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAQ1610821.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DACEUF010000213          223 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.223, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000213 DACEUF010000000
VERSION     DACEUF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.223"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..>223
                     /locus_tag="IXG20_002716"
     CDS             <1..>223
                     /locus_tag="IXG20_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1610822.1"
                     /translation="NSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDT
                     LEETVIHLFRKGITMSEIADLIEKMYGHH"
BASE COUNT           63 a           56 c           53 g           51 t
ORIGIN      
        1 gtaattctcg taacggctcc tatgaccgta cggtcaagac cgagtacggg gaacttcatc
       61 tccagattcc gcgcgaccgc aacggcgagt tcaagcaaca gactgttcct gcttatagac
      121 ggacgaatga taccttagag gagaccgtca ttcacctctt ccgaaaaggt attaccatgt
      181 cggaaatcgc agacttgatt gagaaaatgt atgggcatca cta
//
LOCUS       DACEUF010000214          217 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.224, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000214 DACEUF010000000
VERSION     DACEUF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.224"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..>217
                     /locus_tag="IXG20_002717"
     CDS             <1..>217
                     /locus_tag="IXG20_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1610823.1"
                     /translation="LNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHD
                     VKVLETLSEEANLPNILGDKGYISHKI"
BASE COUNT           77 a           40 c           36 g           64 t
ORIGIN      
        1 tattgaatca aatagccaaa gttggctata actcaacaaa aaaatcttat ttttatggtc
       61 ttaaaattca tatgatcgtt actaagactg gctttccgat tacttactca atcacgaatc
      121 caggtgtcca tgacgtgaaa gtgttagaga ctttatcgga agaagcgaat cttcccaata
      181 tcctagggga caaaggatat attagccata aaatcca
//
LOCUS       DACEUF010000215          216 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.225, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000215 DACEUF010000000
VERSION     DACEUF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.225"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..>216
                     /locus_tag="IXG20_002718"
     CDS             <1..>216
                     /locus_tag="IXG20_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1610824.1"
                     /translation="IAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHA
                     SIEDKMSFICADGETGQLIDVLPTRKL"
BASE COUNT           66 a           39 c           45 g           66 t
ORIGIN      
        1 ttattgcgaa aaattgtcag gtatctttga ccaccgttat tcgtacgttg aaagagttta
       61 aaagctactt accaaagcaa tctaagagga ttctccccag agtattgatg gttgatgaat
      121 ttcgttcgca cgcttccata gaagataaaa tgagctttat ttgcgcagat ggcgaaacag
      181 gacaattaat tgatgtcttg cctacgcgta aattgc
//
LOCUS       DACEUF010000216          215 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.226, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000216 DACEUF010000000
VERSION     DACEUF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.226"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            76..>215
                     /locus_tag="IXG20_002719"
     CDS             76..>215
                     /locus_tag="IXG20_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HAQ1610825.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LK"
BASE COUNT           82 a           44 c           33 g           56 t
ORIGIN      
        1 agacaaataa aaaagccatt tatgttacac tcaaattgta atacccgcta aagcatacaa
       61 ggagagtgaa cataaatgac ttatacccat cttacaccaa acgagcttgt aatgatagaa
      121 gcatattttc atcaagaaac tccggttgct atcgttgcga agcagcttaa acgtggacgc
      181 caaacaattt acaatgtcta taactttctc aaatg
//
LOCUS       DACEUF010000217          209 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.denovo.227, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000217 DACEUF010000000
VERSION     DACEUF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.denovo.227"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            <1..>209
                     /locus_tag="IXG20_002720"
     CDS             <1..>209
                     /locus_tag="IXG20_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1610826.1"
                     /translation="RWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRT
                     PLPKVLCFDEFKSVRGVSGAMSFIM"
BASE COUNT           51 a           43 c           44 g           71 t
ORIGIN      
        1 cgctggtctg tcgtgactcg tctgaaaaaa aatacctcaa tgacagagat cgccgctcag
       61 aaaaatctat cggtctcttc tgtttaccgt attatgaagc gattttatcg tccgttgaat
      121 ccttttcgga caccgttacc taaggtactt tgttttgatg aattcaaatc agttcggggt
      181 gtttcaggtg ctatgagttt catcatgat
//
LOCUS       DACEUF010000218        57895 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.guided.005, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000218 DACEUF010000000
VERSION     DACEUF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.guided.005"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            173..1483
                     /locus_tag="IXG20_002721"
     CDS             173..1483
                     /locus_tag="IXG20_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ1610827.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            1726..2001
                     /locus_tag="IXG20_002722"
     CDS             1726..2001
                     /locus_tag="IXG20_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ1610828.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            2236..3282
                     /locus_tag="IXG20_002723"
     CDS             2236..3282
                     /locus_tag="IXG20_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610829.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            3284..4543
                     /locus_tag="IXG20_002724"
     CDS             3284..4543
                     /locus_tag="IXG20_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1610830.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            4549..5130
                     /locus_tag="IXG20_002725"
     CDS             4549..5130
                     /locus_tag="IXG20_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1610831.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(5212..6087)
                     /locus_tag="IXG20_002726"
     CDS             complement(5212..6087)
                     /locus_tag="IXG20_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1610832.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            6235..6567
                     /locus_tag="IXG20_002727"
     CDS             6235..6567
                     /locus_tag="IXG20_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1610833.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            6679..7887
                     /locus_tag="IXG20_002728"
     CDS             6679..7887
                     /locus_tag="IXG20_002728"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1610834.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            8041..8496
                     /locus_tag="IXG20_002729"
     CDS             8041..8496
                     /locus_tag="IXG20_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610835.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            8858..9133
                     /locus_tag="IXG20_002730"
     CDS             8858..9133
                     /locus_tag="IXG20_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610836.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            9146..11224
                     /locus_tag="IXG20_002731"
     CDS             9146..11224
                     /locus_tag="IXG20_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1610837.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            11384..13270
                     /locus_tag="IXG20_002732"
     CDS             11384..13270
                     /locus_tag="IXG20_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1610838.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            13282..14229
                     /locus_tag="IXG20_002733"
     CDS             13282..14229
                     /locus_tag="IXG20_002733"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1610839.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            14249..14776
                     /locus_tag="IXG20_002734"
     CDS             14249..14776
                     /locus_tag="IXG20_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1610840.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(14839..15492)
                     /locus_tag="IXG20_002735"
     CDS             complement(14839..15492)
                     /locus_tag="IXG20_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1610841.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            15624..16466
                     /locus_tag="IXG20_002736"
     CDS             15624..16466
                     /locus_tag="IXG20_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1610842.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            16624..17520
                     /locus_tag="IXG20_002737"
     CDS             16624..17520
                     /locus_tag="IXG20_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1610843.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            17523..18236
                     /locus_tag="IXG20_002738"
     CDS             17523..18236
                     /locus_tag="IXG20_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1610844.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            18256..18774
                     /gene="msrA"
                     /locus_tag="IXG20_002739"
     CDS             18256..18774
                     /gene="msrA"
                     /locus_tag="IXG20_002739"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1610845.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            18771..18998
                     /locus_tag="IXG20_002740"
     CDS             18771..18998
                     /locus_tag="IXG20_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1610846.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            19150..19701
                     /gene="lepB"
                     /locus_tag="IXG20_002741"
     CDS             19150..19701
                     /gene="lepB"
                     /locus_tag="IXG20_002741"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1610847.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(19784..20326)
                     /locus_tag="IXG20_002742"
     CDS             complement(19784..20326)
                     /locus_tag="IXG20_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1610848.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(20563..20961)
                     /locus_tag="IXG20_002743"
     CDS             complement(20563..20961)
                     /locus_tag="IXG20_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1610849.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            21221..22081
                     /gene="ylqF"
                     /locus_tag="IXG20_002744"
     CDS             21221..22081
                     /gene="ylqF"
                     /locus_tag="IXG20_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1610850.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            22074..22841
                     /locus_tag="IXG20_002745"
     CDS             22074..22841
                     /locus_tag="IXG20_002745"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1610851.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            22897..23754
                     /gene="dprA"
                     /locus_tag="IXG20_002746"
     CDS             22897..23754
                     /gene="dprA"
                     /locus_tag="IXG20_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1610852.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            23876..25954
                     /gene="topA"
                     /locus_tag="IXG20_002747"
     CDS             23876..25954
                     /gene="topA"
                     /locus_tag="IXG20_002747"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1610853.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            25923..27299
                     /gene="trmFO"
                     /locus_tag="IXG20_002748"
     CDS             25923..27299
                     /gene="trmFO"
                     /locus_tag="IXG20_002748"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1610854.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            27497..28402
                     /gene="xerC"
                     /locus_tag="IXG20_002749"
     CDS             27497..28402
                     /gene="xerC"
                     /locus_tag="IXG20_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1610855.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            28439..28987
                     /gene="hslV"
                     /locus_tag="IXG20_002750"
     CDS             28439..28987
                     /gene="hslV"
                     /locus_tag="IXG20_002750"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1610856.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            29001..30401
                     /gene="hslU"
                     /locus_tag="IXG20_002751"
     CDS             29001..30401
                     /gene="hslU"
                     /locus_tag="IXG20_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1610857.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            30419..31210
                     /gene="codY"
                     /locus_tag="IXG20_002752"
     CDS             30419..31210
                     /gene="codY"
                     /locus_tag="IXG20_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1610858.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            31292..32167
                     /locus_tag="IXG20_002753"
     CDS             31292..32167
                     /locus_tag="IXG20_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1610859.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(32299..32937)
                     /gene="plsY"
                     /locus_tag="IXG20_002754"
     CDS             complement(32299..32937)
                     /gene="plsY"
                     /locus_tag="IXG20_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ1610860.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            33064..33498
                     /locus_tag="IXG20_002755"
     CDS             33064..33498
                     /locus_tag="IXG20_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1610861.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            33664..35709
                     /gene="parE"
                     /locus_tag="IXG20_002756"
     CDS             33664..35709
                     /gene="parE"
                     /locus_tag="IXG20_002756"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1610862.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            35721..38171
                     /gene="parC"
                     /locus_tag="IXG20_002757"
     CDS             35721..38171
                     /gene="parC"
                     /locus_tag="IXG20_002757"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1610863.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            38448..40679
                     /gene="pflB"
                     /locus_tag="IXG20_002758"
     CDS             38448..40679
                     /gene="pflB"
                     /locus_tag="IXG20_002758"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1610864.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            40923..41684
                     /gene="pflA"
                     /locus_tag="IXG20_002759"
     CDS             40923..41684
                     /gene="pflA"
                     /locus_tag="IXG20_002759"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1610865.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            41769..42695
                     /locus_tag="IXG20_002760"
     CDS             41769..42695
                     /locus_tag="IXG20_002760"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1610866.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            42877..43770
                     /locus_tag="IXG20_002761"
     CDS             42877..43770
                     /locus_tag="IXG20_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1610867.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            43904..44029
                     /locus_tag="IXG20_002762"
     CDS             43904..44029
                     /locus_tag="IXG20_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1610868.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(44163..44795)
                     /locus_tag="IXG20_002763"
     CDS             complement(44163..44795)
                     /locus_tag="IXG20_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610869.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(44943..45485)
                     /locus_tag="IXG20_002764"
     CDS             complement(44943..45485)
                     /locus_tag="IXG20_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ1610870.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            45620..46009
                     /locus_tag="IXG20_002765"
     CDS             45620..46009
                     /locus_tag="IXG20_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610871.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            46013..46777
                     /locus_tag="IXG20_002766"
     CDS             46013..46777
                     /locus_tag="IXG20_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1610872.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            46906..47853
                     /locus_tag="IXG20_002767"
     CDS             46906..47853
                     /locus_tag="IXG20_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1610873.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            47983..48723
                     /locus_tag="IXG20_002768"
     CDS             47983..48723
                     /locus_tag="IXG20_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610874.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(49014..49682)
                     /locus_tag="IXG20_002769"
     CDS             complement(49014..49682)
                     /locus_tag="IXG20_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1610875.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            49816..50103
                     /locus_tag="IXG20_002770"
     CDS             49816..50103
                     /locus_tag="IXG20_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610876.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            50519..52057
                     /locus_tag="IXG20_002771"
     CDS             50519..52057
                     /locus_tag="IXG20_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ1610877.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            52054..53019
                     /locus_tag="IXG20_002772"
     CDS             52054..53019
                     /locus_tag="IXG20_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1610878.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            53006..54328
                     /locus_tag="IXG20_002773"
     CDS             53006..54328
                     /locus_tag="IXG20_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1610879.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      54391..54495
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            54569..55312
                     /locus_tag="IXG20_002774"
     CDS             54569..55312
                     /locus_tag="IXG20_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1610880.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            55327..56319
                     /locus_tag="IXG20_002775"
     CDS             55327..56319
                     /locus_tag="IXG20_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1610881.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            56331..57068
                     /locus_tag="IXG20_002776"
     CDS             56331..57068
                     /locus_tag="IXG20_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1610882.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            57058..57780
                     /locus_tag="IXG20_002777"
     CDS             57058..57780
                     /locus_tag="IXG20_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1610883.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT        19998 a         9884 c        12112 g        15901 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcct ttttcttttg aaaagcgatt
       61 agtatttcaa taaggaataa aaatacttcg tttaattgac aatctatgct tgaaagttgg
      121 caatgcattc gttaaactaa gtgagattgg aaacttgtga ggaggaaata atatggcaaa
      181 tccaacaatt gcaatcgttg gccgcccgaa cgtcggtaag tctacgattt ttaaccgcat
      241 cgctggtgag cgtatctcga ttgtcgaaga tactcctgga gtaactcgtg atcgtattta
      301 tgcaaaaggt gaatggttag gtcgagaatt cagcgtgatc gacacaggcg gaattgatct
      361 tggcgatgaa ccttttatgg atcagatcaa gcatcaagca gaaatcgcga ttgaagaagc
      421 agatgtaatc atttgtgtag tcagcggacg cgaaggcgtg acagacgcag atgagatggt
      481 tgcgaaaatt ttatacagaa gtaataaacc agtgatttta gcagtcaata aagtagataa
      541 tccagaaatg agaaacgata tctatgaatt ttattcctta gggctaggcg atccttatcc
      601 tgtttcagga agccatggac tagggattgg tgatatttta gatgaagcag tgaagtattt
      661 ctctgaagag actgaagaag aggacgacga cacgattaag tttagtttga ttggtcgccc
      721 aaatgttggt aaatcctctt tgatcaatgc gattttagga gaagaacgtg taattgtttc
      781 ggatattgaa ggaacgaccc gagacgcaat cgacacccac ttcgtttctg aaagtggtca
      841 gaaattctta atgatagata cagctggtat gcgtaaacgc ggaaaagtat acgaaaatac
      901 tgaaaaatac agcgtgatgc gtgcaatgcg tgccattgac cgttctgata tcgttttgat
      961 ggtactgaat gctgaagaag ggattcggga acaagacaag cgagtagcgg gttacgccca
     1021 tgaagctggt cgtggaatta ttatcgtcgt gaataaatgg gatcttgtaa agaaagaaac
     1081 caatacaatg cgtgatttcg aacaagagat tcgtgatgaa ttccggtatc ttgattacgc
     1141 tccaatcgtt tttgtttctg cagtgactaa acaacggttg gaacgtctgc ctgaaatgat
     1201 cgaacaagta agtatgaacc agaatcttcg tatctcttcg gctgtgttga atgatatcat
     1261 tatggatgct gtagcaatca atccaacacc aacagataaa ggaaaacgcc tgaagatttt
     1321 ttatgctaca caagtcgctg tcaaaccacc tacatttgtc gtttttgtca atgaagagga
     1381 attgatgcat ttttcttatg aacgtttctt agaaaatcaa atcagaaaag catttacttt
     1441 tgaagggacg tccatccgaa ttatccctcg taggagaaaa taggcatttt accatatcga
     1501 cgtttttttt tcaaaagaaa aagacagttt cgtttgttac gtttctatga ctaaaaaaag
     1561 catgtgaaaa ttggtaaaaa ccttgctatt aagcggttca tatgatatcg tgataacaga
     1621 atattgaagt taatcaatat ttctttttaa acttctggac cactcagaaa gttttaacta
     1681 aatgatattt ctatcatatc tcttgtggag gaggtgaatc tatccatggc aaacaaagca
     1741 gaattaatcg aaaaagttgc ttcagctact gatttaacta aaaaagatgc aactgcagca
     1801 gtagatgctg tattttcaac tattcaagat gctttagcta acggcgaaaa agttcaatta
     1861 atcggttttg gtaactttga agttcgcgaa cgcgcagctc gcaaaggtcg taacccacaa
     1921 actggtgaag aaatcgaaat tccagctagc aaagtacctg cattcaaacc aggtaaagca
     1981 ttaaaagacg ctgttaaata agacagttga aaaagcgctt gtggctctag ggctacaagt
     2041 gcttttcttt ttatttccta gcagaaaagg aataaataag agacagagat tccgaatccg
     2101 tatgtcatac atagaaaaca catgtatttt atttaatttt tatttatttg caagttatga
     2161 ggtgagcttt gctcttatct tttctagaat ttgcttacta atggtaaaat aaaaataaga
     2221 agaggaggga tgcgtatgga acttgattat caagtagaag atgcgttgac gaaagtgttt
     2281 tacggagaaa cgctagtcag tcagctaaga gaactttcca ttgatcagcc aatcttattt
     2341 ttaacgaatc agcgctatta tgatttgttt gcagataaga tcaacccgtt atttgcaaat
     2401 caagcaaaca atgactggta tatttgtgcc aacactcaat gcaatcattt aacagaatta
     2461 aagaacctgc ttgatttcgt caaacgctat cccgaaaatc aatccatgtt tatcatcgga
     2521 tttggaaatg aagggatcat ggatctggca ggtttttttc aaaaacatac aacccttgat
     2581 tcatcactat ggatgatccc tgtatcgatc cgttctctaa gccgagcatt aacaccgcag
     2641 cggacgattt tgcagctacc aaatcgtgta gtgctgcaaa ccatcaatct gccagaacgc
     2701 atcgtttatg atcaaacaat cagcgagcgg caggtggacg gaaagcagat tgactttttg
     2761 acttttatct gttgcggaat gatttgtgat catcgttttt tgcaaaatct gtataaaaac
     2821 tatccaaata aaaagcaatt aacgactcgg ccgtttgccg ggatgatcga agaaatgatc
     2881 tatttttatc aattagaggc agaaaacctg tcaaattatg gtaaactgtt tgaacaagct
     2941 ttttatctga ccgaaaatgg ccatctactt tctgctagta tgaaaaaatt tttaggaatg
     3001 atgtttcatt tcgtgtggaa tctccaagca tatccttttg atttccaact aaaaaacttt
     3061 tttgtgtggc tggacttgct tggctatcca atccactggc tggaacagat ttcaaagatg
     3121 gagtatttgg aaaaagttct ttttttggca gaaaaaagca aaaaaatact tgttttagaa
     3181 aaaataggta taatagacgg ataccaacat ccaaacgaaa aagaattatt gagaacaatg
     3241 acggtatttc aacaaatcgt cagtgagata agagggaatt gaaatgaata caaatagcga
     3301 aaaaatgttg caagctttat cagatgaaga cttgtcacaa gcacaaatct ttttagaaaa
     3361 agcattaaaa gaagacccag cagatatatt agctgaacta ggcgaagaat tgctggctat
     3421 tggctttttg atcgaagcaa aacagatttt tgaacaactg atcaaccaat acccagaaaa
     3481 tgacggactg aacatcccgt tagcagaaat tgcaatcgaa gataatgaga tcgacttagc
     3541 ttttgaatac ctggaaaaag tggataagca aagtgattac tatccgcaaa gcttattggc
     3601 gatagctgat ttgtaccaag ttatcggaat ccctgaagtg agtgaagcaa aattgaaaga
     3661 agcagctggg ttgcttcctg atgaaccatt gatccagttt gcgctagctg aattgtattt
     3721 ttcagttgat cgtttcaaag aggcagcagt ggtttatgaa acattgttgg caagcgggat
     3781 aaatgaaatt tcaggaatct cgatgcagga acgtttagga caatccttaa gtatgcaagg
     3841 agaattcgaa gcagctatcg tacctttaga agaagcactg aaagaagaaa ggacagatga
     3901 tcgactgttc cagctagctt ttacgaatct tcaattgaaa gaaaatcagc aggcaatcaa
     3961 ctatctggaa gaacttcgag aagttaatcc gcattatcaa tcgttatatc tatatctagg
     4021 acaagctctt caagaagaag aaatgattga agaagcacaa acagtgttag aagaaggaat
     4081 caaagaaaat ccttaccaag tcgaacttta tcacctggcc tcggaaaatg ctttccgtct
     4141 gcatgataga cagcgagcag aagaactttt actgaaagct ttagaagtag gagacaaaca
     4201 agatgaaaca ttacttactt taagtaatct ttacttggat gatgaaagat atgaagatgt
     4261 gatacaagtg atcaatcaga tggaagaaac tgctaatcct tatgcagaat ggaacctagc
     4321 ccatgcttat aatgaattag aagactttac cgttgcagct gtacactatg aacaggctta
     4381 tcatgaacta tcacatgaac cagatttctt gaaagagtat gcctttttct taagagaaga
     4441 agggcagctg aaaagagcgc aagaattatt gacttattat ttaacacttg aaccaggaga
     4501 tatggaggca ctgtcattat tagatgacct aacagagagg tgagaaaaat gctaattgat
     4561 gtacgggaaa agaagaattt tttaacgtgg atggtcaatc atgtatcttt tagccgaaga
     4621 gaagtttttt ggatcttgaa ttatcttgcc aatcatgaag cgattttaag caatgtccac
     4681 tttgttgaag gggcgaattt tgcggatcga ggactgcaga tcagtgatct atctgttgaa
     4741 ggggaaccca tgaaattgtt tttgcaggga aaagaattta cggacacaga ccagattttt
     4801 catgaaatcc gtttgaattg gaaaaagcct ttatatgtgg aatgtatatt cgaaaatgct
     4861 tggcagacaa gagaatatct atctatctta gaagacaatc cattttcttc ttgggattct
     4921 tcagtcagtg aagaagatac tgaattaatt gaacgatatt ttcaagaaaa agaagaagag
     4981 gcaaaactaa aaatgcttta tgctcaaatc gatcaagcac tagaagatgg agatagggat
     5041 gcttttttga aattatctga tgaagttaac cgaaagatcc ttttgcagac acaaaagaaa
     5101 agtaaccggg taagtgaaca taacaattag ttaaaaatag tatagtacta aaaaagacaa
     5161 aggtcagctg gatgtggaat ctttcctcat agcctatctt tgtctttttt cttatagttt
     5221 tttcagtaat cttgtctgtg gcttcaaata agtaaagcct tgcccttcca cttcatgcac
     5281 attgatgatt gaaaggaaag ctttttcgtc aatctcgtgg acgatcctct tagcttcagt
     5341 taattcacta gagctaacga ccatatagat gatttttttt gaattaccag agaatgcgcc
     5401 ttctccagaa aggaaagtca cacctctttg cagaccggtc atcagtagtg gtgcaatctc
     5461 ttcgcttttg ttggagacga caagtagacc ttttgcagaa tatccgccat ctaagatcga
     5521 atcaatcaca cgactgaaaa catacgaaac aatcaacgta tacatcatac gtttcaagtc
     5581 gatataggtt aatgaagcaa gtaagacgat gatatcaaat gccaataggg aacgccccat
     5641 agtgatgccg taattttgtt ctaaaatccg agcgataata tcactgccgc ctgttgttcc
     5701 acccatgcgg tagacgatcc cgcttcctac acctgcaact aaacccgcaa gtagagaaac
     5761 gatcagtaga tcatgctgta aattgatttc tagtggaacc ctttgccaga tccataaaaa
     5821 gaatgacaaa gaaagtgtac ctagaacagt ataggccaag gaacgtttac cgaggatctt
     5881 tcctcctagt aaaatcaatg ggatgttgat taataaagtc gtataagctg gatcaatacc
     5941 aaataaagct cttaagatca aggtgatccc tgagactccc ccttctgcta aatgattagc
     6001 gatgttcaag taaacaagac caaatgcata aatacttgtt cctataatga taaaaagaac
     6061 ttcttttact gtttttcgtt tgaccatatg aattccagct ttcaattatg tattttcaca
     6121 gaatttccat ttagaaattc cttcctaaat gtagcatgga aattcgattt tctcaattgc
     6181 tcttatctag gatttgcgtt aaagttttgg taagatagtt ttggagggaa aaagatggat
     6241 aaaaaacgtt ctttatacag tatgcagcag gaagtggatg aatatatcca gcaattcaag
     6301 gtggggtatt tctctccatt agctcagatg gctcgattaa ctgaagaagt aggtgaattg
     6361 gcaagagaag tcaaccacac ttatggagaa aaaagcaaaa aggcttctga accagttaac
     6421 tcggtagcag aagaacttgg tgatgtttta ttcgttacga tgatcatggc taattcattg
     6481 aatatcgact taactgaagt ttttgaaaaa aacatggaaa aattcaatca aagagaccat
     6541 aatcgctttg agcgtaagga cgagtaaaca agatgaagtg gtcctggcct tgaagactct
     6601 gaaacggact tgtcaaacag tcaccatgca actgagtctt tatctcagga caagaaacac
     6661 gcaggaaagg ttggaaaaat gaaattaaaa caattacctc tggaatacca aaaggcaatt
     6721 ccagttctga aaaaattaga gaatgcagga tatgaagctt attttgtagg tggaagtgtt
     6781 cgtgatatct tgctgaacca gccaatccat gatgtggata tcgcaacaag cgcatttcca
     6841 gaagaaatca aacagctttt tccaaaaacg atagatattg ggatcgaaca tggaactgtg
     6901 ttggtattag aaaatgatga acaatatgag atcaccacat ttcggacgga atcaacttat
     6961 caggactacc gtagaccaga tcatgtcgaa ttcgttcgtt cgttggcaga agatttgaaa
     7021 agaagagatt ttacgatcaa tgcgtttgct ttgaaagaag atggagaaat catcgatttg
     7081 ttcgatggtc tgcaagatct agaagaacag atcttgcgag ctgtaggcaa tcctcatgag
     7141 cggttccatg aagatgcctt acgaatgatg cgcgggttgc gttttgtcag tcaactaggg
     7201 ttcaaattag aggaagaaac cttctacgcc atcaaagaaa accacgagct attaggtaaa
     7261 atatctgtag aacgtataaa tgtggaattc atcaagatgc tcttgggaaa tgaacgggcc
     7321 gcgggaatcc atgcatttat tgaaacagag tgctacattt gctgtcccgg tttgaaagaa
     7381 gaaggcgaag cacttttacg ttttgctgaa ttaccatccg tcccattgaa agaagagagc
     7441 caagcatggg cattattgat cagccaactg gatctaaaag aaaatgagat tcgttccttt
     7501 ttgaaatcgt ggaagtgttc taaccaaatg attaaagacg ttcaagcact tgtccaagga
     7561 ctacgacaac gagaaactgg agctttggaa ccattcgaat tgtacttctt aggcaaaaaa
     7621 catgcactgc aagtagaaga attgatgatc tattttgacg aaacacctca gatggatgcc
     7681 gtaggagaat cctatgatcg tttacctatc aagtcattaa gcgaattagc agtagatggt
     7741 aaaatcttgc tagaagaaac tggaaaaaaa cctggtaagt gggttagtga agctttgcaa
     7801 ttagctgaaa aagccgtcgt agaacaaaga ataaaaaatg ataaaaaaca ggttttggat
     7861 tttttatcga aagaaaaatt gatttaaaaa acactagact gtatctgctc agctttgttg
     7921 gctacttaaa gtgggcagat acgtcattag aagaaaaaac ataagatgtg accacttaca
     7981 taaatttgtg ctaaactgag gtagtacaag aataaattac atcataaagg agtcttttta
     8041 atgagtaaag aaatgacagc gctaaaattt tattttagaa atggagaaac ctggacgatc
     8101 aaccgccgtc atatcggcga tctttggatc aaacaaatca caacaagttt tggacgaatc
     8161 aatggcagtg aatttgttga aatccatccg tgtgctggat ttaaaatcga aatcttccat
     8221 gaaggggacg cagttgcgac acatgatatc aacctaggcg gactggaaat gggcatgttt
     8281 aatcgcgcat tgaaatacga agatatcgag cgaatggaaa tcctttaccg aaatggcaca
     8341 cctgatcttg tgtacttccc atatctagat aaaggaacag aaggattaga caatcaatat
     8401 caatcaacaa aaatcagcga aaaaacaggt aacttgtata tcgtgatcaa tccagatcaa
     8461 cgtgtagaag atgtatacgg ggaatttttc gaataaatcg atttttacgc aaaaatgagg
     8521 ctgtgaaaga gatgtgtctt attactactg ataggctgtc tcttccacac cgtttattca
     8581 gaataaggga ctgtgacaag atttttgtcg cagccccatt tttttgttgt gataaaagaa
     8641 caagaaaggc aggaatggga aagtaaacat ccttttgtca gaaactttta tctttcttaa
     8701 gttttacttg atatctgata ctatttggaa tagaatgagg cgttgaaaaa tttatggaaa
     8761 tttctaacta gcaataaacg atgaaacaat gaaatatttt tcatccaaaa agaactttgc
     8821 tataatgatg gaagtttgtg tataggagat ctggaaaatg agagagagtt ttgaacaaca
     8881 aaaaaaactg ttgcatgatc gctatggcgc cctttcgatg gatgaccgcc gtcaaatact
     8941 ttgtaagttg cgtaaaagaa atatattgat gtatcgtcag ttggaacgtc tgaaacatga
     9001 tctgcttcgt ttggagtcaa aacgtgtaca gtgtgaatta gaggggaacc aaacacaggt
     9061 tgaagtagtc gaaaccaaaa ttttaaagaa aaaagaacaa tttttaaaaa tgcttacaca
     9121 aaataaaaaa taaaggggat ggacgatgga atttgctgag ttagttattg tatttgcagt
     9181 aacgatcacc ttatcgaaca tagctagccg cattttgcca atgattccag ctccactgat
     9241 ccaaattttc cttggagtgg tattaggtct gacagagtgg ggacaatcaa tcgattttga
     9301 acctgaattg ttcttagtta tgattattgc accattgtta tttagagaag gagaaaaagc
     9361 agatatttct tcgattctca aaaatttcgg gacaattttg ttcttggcat ttggaggagt
     9421 cattttgacc cttgtcgggg taggtgcgac gttatcattc ctgttgccaa gtgtcccact
     9481 tgctgcctgc tttgcttttg gagcagcgtt aggtccgaca gatgcggtag cagtcagttc
     9541 tctttctgga cgagtgaata tccctaaaaa ggcgatgcat attttggaag gagaaggtct
     9601 tttgaatgat gcatctggtg ttactgcctt ccagtttgct cttggtgcgc tgatcaccgg
     9661 atctttttca gcagtaaacg cggggatgag tctagtagtt tctagtattg ggggagcttt
     9721 gatcggcttt ttgcttgtct ggttcaaaca aaaaataatt catttgatcg aaaaagcatc
     9781 agcacaagat gtcacagggt atctgctgat cgaacttttg cttccttttc tagcctatgt
     9841 attggcagag gcatttgatg tatctggaat cattgctgca gtggcagcag gtattctcca
     9901 agcttctggt tttaggaaaa tctctgtgtt tgatgcagaa ttgtcgagct tgtcccacag
     9961 tacatggaca actattgctt ttacactgaa tgcattagta tttatatttt taggaatcga
    10021 attgacgcaa gtgttctcgc cagtatgggg agacggttta tatccaaacg gtcttttatt
    10081 agcgattatc gtattaatct ctgtcatgct ttttgtgatc agattcattt ctatcagtct
    10141 tttttatgta ttcaaagatg gcagtaaaaa gttcaaaaaa cagttgaacg agatcttgat
    10201 cttaacattt ggcggtgtaa agggaacagt cagtttggct acgattttta ttttgccatt
    10261 ttcaatcaat aatatgatgt tttaccagcg gtctttgctt ttgtttctga cggcgggtgt
    10321 catcttagtg actttggtca tagggatcat tgtcttgcct atgctgacgg aaacagagga
    10381 agcgaaaagc acggatttga atgctttgat gattttagaa gaggtcgttg aagttttgcg
    10441 aaaagaaata aaggaaattg atcgtaatac aaaagaattt ttagccactg aagcagtgat
    10501 agaaaattat caagaacgga tacgtgatct gtacttggaa gatttaaccg atgatgaaaa
    10561 gcaagaagta caagaaatac aagcactgat tttgtcgatc gaacgggatg gattggatga
    10621 aagttatcgt tcgggcaagc tttcttctaa tggttatcgt ttttactccc gttttctttc
    10681 tcgattcgaa cattctatca caagccagat tttgtctttt atcggctttt ggttcattgt
    10741 tgtccgtagg ttaatgcgaa tcgttctcca tccgaaaatg ttttggcaaa gaagatatgc
    10801 tgatcgtcag acatttatta gtgatgaaga tatccaagaa atcagagaga catatcaaaa
    10861 aaatactcag ttgattattg aaagtttaga taacttgact gatatttatg atgaaacgat
    10921 ggttcaattt tttatccagc aacgcaaact tgaaggaata aaaatgaact cggggaattt
    10981 gatttcttcg tggatgatcc agcaagatag tctgtttacg aaaaaaatgc taagaggtta
    11041 ttatcttgaa agaaaaatca ttgatgaata cgaagttgct gaaaaaatca cgacgttttc
    11101 tgcgaacagc tatcgcagga atatcaattt gttagaatct tatacgatga acaaaccatc
    11161 tgataacttt tctttccgat ttgcgtttcg agcaaagtca aaaaaaacga gtccaataaa
    11221 ataaaaaggt aaaaattttc aagttttata aaaaacaaaa catatcagaa caggatcgaa
    11281 tcggtcctgt tctttttttg agaacacaaa aagaacaaat accaaaaaaa ttttcctgaa
    11341 gttcactatt tttatgatac aatatccaag ataaggaggg aacatgttga aagaattaaa
    11401 agtaactgac ctcaaaaaaa cttatggtga aaaagatttg tttgaccaga tttctttttt
    11461 ggtccacgaa aaagaccgta tcgggttgat tgggacgaac gggacgggta aaacaagtct
    11521 cttaaatatt attgccggta tcgatagcag cgacggtgat cggcaaacag tgttttatcc
    11581 aactgattac cgtattggtt atctttctca agcagctgaa ttttccgatg aattaactgt
    11641 tctccaagca gtctttcaag gaaacagccc cttgatccaa actgttcgag catatgagga
    11701 agcattgatc gaactagggg aaaacggaga agaccctgat gtgcaaaaac gttatgctaa
    11761 agcagaagaa caaatgaata aagaagatgc ttggactaca gataccaacg caaagattat
    11821 tttgcaaaaa ttaggaatca gtgaattaga taaaaaaatc agtactttat caggtggtca
    11881 gaaaaaacga gttagtctgg cgcaagtgtt gattgatgaa cccgatttac tattactaga
    11941 cgaaccaaca aaccatttag attatgaagc gatcgagtgg ttggaaaact atttgaaaca
    12001 ataccgtggc gcactattga tggttaccca cgatcgttat tttctagata gagtagcaaa
    12061 ccgaatcttc gaactttcat ttggaaaatt atacgagtat aaaggaaatt atgaagctta
    12121 cgtcttagaa aaagcagaac gtgatcgtgt agaagtagaa caagaagaaa agcgcaagcg
    12181 gctttataaa caggaattag cttggatgag ggctggcgta caggcacgtg gcaccaagca
    12241 gcaagcacgg atcaaccgtt tcgaagatct aaaggagaac ttgtaccaag taaaccaaga
    12301 agatgattta gagctgaacc tagcgacaca acgtttaggg aaaaaagtac tggaaatcaa
    12361 agatggcagt taccgtataa atgaccaaac actgctagaa catttagatt tattgattca
    12421 atctcgggaa agactaggta ttactggtaa aaatggtgcg ggaaaatcga cattgcttaa
    12481 tattctagct ggcagaattc ccctggatag cggtacaatg tctatcggtg aaacggttca
    12541 tcttgcgtat tatacacaag aaaatgaaga aatggcacca gataaacgga tgattgctta
    12601 cttacaagaa gctgctgaag aagcaaaaac agcagatggc agtcaaatcg gagtagcaga
    12661 attacttgaa cgatttttat ttccaagatt tatgcatggg acactgatag gcaaactttc
    12721 aggcggagaa aaacgccggt tatttttgtt gaaactgctg attcagcaac ctaatgtttt
    12781 attattggat gaaccgacca atgatttgga catagcaaca ttgacgattt tggaagatta
    12841 ttttagaagt tttccaggtg ctgtgatcac tgtttcccat gaccgttatt tcttggacaa
    12901 ggtagcagat aagttattag tattccaagg aaacggaaaa caagaacttt attacggaaa
    12961 catgtccagt tatttactga agcaaaaaga gacccagcag cctgctgaaa aagcgaaacc
    13021 aaaaatacag agcaaggaac ctgctgaaaa gaagaaatta tcttacatgg aacaaaaaga
    13081 atgggaaacg attgaagatg agattgcaga acttgaagaa aaaatctcat tgctccaaga
    13141 agagatgaat catcaaggag ataatttcac tcgattacaa gaattgcaaa acgacgtatc
    13201 tgaaacagaa gcacaacttg aagaaaaaat ggcacggtgg gaatacttga gtgaatgggt
    13261 agaagattag gaggaaaaag gatggaacaa gcgtatcttg acttaggaag aaaattgttg
    13321 gaagaagggc atcgtaaaga agacagaaca ggcacaggca cattaagctt attcggatac
    13381 caaatgcggt ttgatctgca aaaagggttt cctttgttaa caacgaaacg agtacctttt
    13441 ggattgatca aaagcgaatt gctttggttt ttaaaaggag atacgaatat ccgctatcta
    13501 ttacaaaata aaaaccatat ttgggatgaa tgggcatttg aacgctacat caaaagtcca
    13561 gactataatg gaccagatat gactgatttt ggccatagat gtttgacaga tcctgaattc
    13621 aatgaagaat ataaaaagca gagcaagctt ttttgcgaga agatcctaac cgatgattcc
    13681 tttgctgaaa catacggcga tcttggacac atctacggct atcaatggcg tcactgggaa
    13741 acaaaagacg gtggctttat cgatcaaatc aaacaagtga tcgaagcaat caaaaagact
    13801 cctgattcta gaagattgat cgtttctgca tggaatccgg aagatgttcc tagcatggct
    13861 ttgcctcctt gtcatacgat gtttcaattt tatgttcaag agggacggct aagctgccaa
    13921 ttgtatcaac gcagtgggga tgtgtttcta ggcgttccgt tcaacattgc aagttatgcg
    13981 ttgctgactc acctaattgc acatgaaaca ggtttagcgg ttggcgaatt tgttcacaca
    14041 ttaggagatg ctcatcttta tatcaatcat atcgaacaaa tgaaagaaca attgagccga
    14101 gaaatacgcg catttccgac tttagttttg aatcaggaaa agcagtcagt atttgatttt
    14161 gagatggaag atatccaatt agaaggatat gacccacatc cggcgatcaa agctcctatc
    14221 gctgtttaga atcagaaagg atgagaaaat gttagctgca atttgggcac aggatgaaaa
    14281 cggattgatt ggaaaagaag accgtcttcc ttggcgtttg ccaaatgatc tgaaattctt
    14341 caaacaaatg acagaagcaa atacactagt gatgggacga aaaacattcg aagggatggg
    14401 tagccgtccg ttgccgaatc gtcagacgat tgttttgacg agagattctt cctataaagc
    14461 agaaggcgtg catgtcatgc atagtgtgga agacgtgctt gctctggaaa aagaaacaga
    14521 cggtatcttt ttcatcgctg gagggtcagc tgtttatcaa gaatttctgc cattttgtac
    14581 gatattatac cgtacagtca tccatcatgt attcgatgga gatgcttatt ttccacctgt
    14641 tgactggtct gattggtcac tgatcaatac tagtcaagga gaaatggatg aaaaaaatcg
    14701 ttatccgcat caatttgaaa cgtatcagcg taatgagaat cctgtaaaaa gcgggacagg
    14761 cgaattgacg atataaaaaa gctatggata gactagctgt tatttagagc tgtcccatcc
    14821 atagctttat tttttttatt aagtaaagaa taaaacagag aagtacataa aacctgcacc
    14881 taacataaca aacaaatgcc aaacaacatg catatagcgg atagaattca agctataaaa
    14941 gaatgcgcct atcgtgtaag aaagcccgcc tgctaccatc aaaataacac cagtcattcc
    15001 aagagagtga taaagaggaa cagcagccac gacacagagc caccccataa aaatatagat
    15061 gatcgtagat acttttgata ctctctcccg tttatgcaac gtcaacgctt tatatataat
    15121 gccaagaatc gcaaacaacc agattaatac aaataaagtc caacctagcc atcctcggat
    15181 actcagcaaa caaaaaggag tataacttcc tgcaatcaaa agaaaaatcg aactatgatc
    15241 gaatacttgg aacacctttt ttgcttttgt aaaaatcaat gaatgaaaca aagtagacgt
    15301 taaaaataaa agaatcatca tcgatccata gatagcataa gagacaatat ggagggagga
    15361 atgcaagtgg gcacctttta ctaaaagaac cactagtccc gcgatactca atccagtccc
    15421 aattccatgg gtgactgcat taaacacttc atttaatatt tcatacgttc gattaaattt
    15481 cgttttttcc atgaaaacgg ctcctttact aggtgtaatt aatgaaaaat cattatttgt
    15541 ctgttatact aaaaatagaa attgtcttac atgtagttta acatgaataa ggcatcttgt
    15601 taaagaaaga gagagagaaa ataatgacaa atgtaaaaat cgtaacagat tcttcatgta
    15661 cgatggaaaa aaatgtacgc gatgctttag gcattcgtgt catgccgcta tccatcatga
    15721 tcgacggaac agtatatccc gatgatgacc aactagacgg cgaaagattt atggaaatga
    15781 tggcgaaagc aaaaaatctg cccaaaacaa gccaaccacc aattggagaa ttcatctcga
    15841 tgtatgatga gctaggagaa gacggcagtg aagtgatttc gatccatatg acaaaaggct
    15901 tgagtggaac agtagaagca gcaagacaag ccagtaatct aacaaaaacg aaagtaacgg
    15961 ttatcgacag tgataccact gaccaaggat tatcttttca ggtaattcga gcagctgaat
    16021 tagcaaaaga aggtaaaaca gtagagaaaa tccttgaaga agtcaaaaaa gtacaagaca
    16081 atacaaaatt atacatcgga atatccacac tagataactt ggtaaaaggt ggaagaatca
    16141 gccgaacaac tggtttgctg tctagtgttt tgaacatacg cgtggtgatg aatttcgccc
    16201 attctgaatt aatccctgta actaaggggc gaggaaagaa aacgttcgat aaatggttta
    16261 ataatttgaa aaatgaactg aaagaattgc caaatgtgcg acaaatcggt atttcacatg
    16321 cggatgcaaa agaattggcg caagaattca aagagggttt gcaagaatta ttcccggaca
    16381 tgcatatccc tgtactgcat acgaatccgg ttatcgctac gcatactggt aaagacgcgt
    16441 ttgcaatcat gtattatgta gattagtcga aaagaggata agccctcagc ctgaaaggct
    16501 tgcagcttat tctctttttc tttataaaat taggaaaata attgttttct tttttcataa
    16561 agactacctt tttgataaaa tttttgataa cattagaacc aatcgaatac aagaggtgaa
    16621 aaaatgaaat taacgaaacg acatttgatc ctcgttttgt tcccattatt aattgcgatc
    16681 atcatctatg caggtttagc tgcttctata ccgaaagctg gcccactaat gaaaccagta
    16741 actactgtga gagaagatcc aaataaaaaa gaaaaacttc actacatagc tatcggagat
    16801 tcgctgactg aaggagtagg agatcaaacc aagcaaggag gattcgttcc ccttgtcgca
    16861 aatgatctaa aagaccgtta taatttgacc gcggttgaaa cagaaaacta tggtgtaaat
    16921 ggtgaacgaa gtgaccagat cttaaagcga gtaaaaacaa aagaagaaat tcgaaacaat
    16981 atcaaaacag ctgattttat cacactgact gttggcggta atgatttgat gaaagttatc
    17041 caaaataatt tattcgggtt aacggtcaat tcctttaaaa agccactgaa aaactatcag
    17101 gaaaatgtca cagaactatt ggaagaattt cgatccctca ataaaaccgc acctatttac
    17161 attttaggca tctacaatcc attttatttg aactttcctg agatcactga catgcagaca
    17221 attgttaaca attggaacaa aggaacagaa acggtgacaa aaaaaatgcc gaactgctac
    17281 tttatcccaa tcaatgattt gctttatcaa ggattgaatc aggaagtagg tgtcacagaa
    17341 gaaggaacag atcaaacaac tagcacgaca gaaaatctaa atggcatcaa aaataatgtg
    17401 ctctatgaag aagatcattt ccatccaaat aatttaggtt atcaattgat ggcaaatgca
    17461 gtgagagatc agctgatcga gacacaaaac caatggttag agaaggagga tgatcaataa
    17521 acatgaaaac aagatctgac gtatcttcaa acaaaacaaa gaaaacaaat tactggaaat
    17581 tcgccttcct tatccttctt ggggtagtac ttggttcgac cgtattttta ggcagccgta
    17641 tttttgctaa tcgggaacca gaactcccag aaataccagc tcttacggaa agacaaggag
    17701 atcctgtctt aacgatcaat tcaaataaag aaaaagtcaa tcaaatcatc agctttttcc
    17761 ttagtgagta tcaaaaagat agtgatattg aatataaatt ttatttggaa aatgaagcac
    17821 tactcaatgg tactttcgaa gtactaggtt ttcctatcga tttctattta tattttgatc
    17881 cgtatgtgat ggaaaacgga aatgtccaac taaaagctag aagtctttct atcggaacat
    17941 tgagtttgcc aatcaaagat gtgatgaata tgatcaagag aaactataaa ttaccagatt
    18001 ggatcgaagt caatacagat gatctgacta tcatgcttag gttggatcaa ttccgaatgc
    18061 aaaacgggat gtatattaaa gcagataaaa tcgacttagt aaatgatgac attcggttta
    18121 gtctatattt gccaaaagat acctctgatg cttcaaacaa ctcaaatacc aacaaaacta
    18181 gcaccagcag tactgaaagc aagacaaaag aaaataaaaa atcatcggat gagtagtatt
    18241 agagggggaa tcataatgga acgagcaata tttgcaggag gctgcttctg gtgtatgatc
    18301 cagcctttcg acacgcttcc tggtatccat acagtgatgt caggatatac tggagggcat
    18361 gtaccagatc cgacttatga acaagtaaaa agccaaacga ctggacatac agaagcagtg
    18421 gagattatct atgatccaac attgatttca tatgaacaat tagtagaact ttactggcag
    18481 caaactgatc caactgacgc tttcggacaa ttcgaggatc gcggtgacaa ttatcgcccg
    18541 gtgatctttt atttgacaga agaacagcgg atcattgctg aaaaaagtaa gaaacagtta
    18601 gctgaaagtg gacgtttcga acagccaatc gttacaaaaa tcgaaccagc ccaaccattt
    18661 tatctagcag aagaagacca tcaagagtat tataaaaagc atcctgaaaa ttttaaaaga
    18721 aaccatgcac gccgggctgc ctttatcgaa gaaaattgga ggaacgaagg gtgaatcgaa
    18781 gcttttttca ttatttaatg acactgcgag gcggtggcac acatgatgct ttatctgttt
    18841 tcgctacaga agccggtaaa gacatccaat ttcctaagca cagctctagc tacgaagaaa
    18901 tttctaatta tctggaactg aatgtcgact atttgccgtc catggatatc tttgacgaag
    18961 catgggaaaa atatttagaa aataatcatg acatataaga gatagttttg tactttatga
    19021 tcaaaaaagt tttttgtcca atagcctaat tgaaagaatt atttttattt cagctatcta
    19081 gtcgcatttt tttgatattt ttcttataat aaagaaagaa tgaaaaaaag agggatggat
    19141 tgatagagat tgacaaaaaa acaacgctat attaatcgct tctggcttgt attcaaatac
    19201 ttacttgttt ctgtattcct tgcgttcatg ttgcggggat ttttattcat cccggtacca
    19261 gtggaaggca attcaatgga aaacgtgttg aagcaaggtg atatggttgt gatggaaaaa
    19321 ttttcggaga tcagacgatt tgatattgtt gtttttcaat tagcagatgg cacgatttat
    19381 atcaaacgag taatcggtct tccaggagaa aatgtcagct accaaaatga tcaattgaag
    19441 atcaatggga aagtagttaa ggaaccttat ctaactaaaa acctgaaatc agatcatgca
    19501 aatgcatcct atacgacgga ctttacacta caagagctaa cgggacaatc caagcttccg
    19561 gaagataatt attttgtact tggagataat cgaagagttt caaaagatag tcgttcgttt
    19621 ggcacgatca ataaaactga tattctaggt aaagcaagat ttgtctatta tccacttgat
    19681 gaaataaaat ggatccagtg agaaacaata aagcgtgcag tctgttgaga ctgcacgctt
    19741 tattgtttaa tccaaaatca gtttactacc ttagtacgta aaatcaaaca gctcgtttaa
    19801 acacttttct agcctctaaa gttttgataa atgaataaac aaccattatt tgaataggga
    19861 tttggatcat attcctgatg aatctaccta gaaaaattac tttgaaatct gcaccaaaca
    19921 aaacagacat cagccaagaa ttgataaaaa ggctgactgg gatcataatc aataattggg
    19981 cagcgatgac ggaaggcagg tttatctttt tatgatataa aaataagcca ttaataaatc
    20041 caccaaggaa agccgtgata agaaaagcag cttgaaacgt tcctgaacca aatatcgtga
    20101 aatttaaagt gttcgacaac atttcaagca aaccagccca gatcggtcca tatagataac
    20161 ccataagcgc catacttaaa aatccgaatg tgatttgaaa ataagcagga ccaatcgcta
    20221 tcttgtataa gatgacatca agggctaata aaagagcaga ggtcgacaac aatcttgcat
    20281 tcatttcggg caatctaaaa acgtcttttg caatcaaagt gttcaaaata ttctctcctt
    20341 tgtcagttaa taataaaagg ataccataaa atcaccaatg tgtcatgaca cattggtgat
    20401 ttacatacac tggcatctaa caattaaaaa aagggtgaga agactgcttt tataaactgt
    20461 aaaaaagaga ttgtacgaat aaaaaaaata gaggcggaga atacttatcg tatcatccgc
    20521 ctttgttttt ttgttataaa aaacgtcaga ttagttatct gcttacatct tcatccatac
    20581 gggttctaat gtatttccgt ctagatcttg gacttctaat gaaaacattt cttcttcagg
    20641 cattcccata tctacttcgt ggaaagtacc accattttct tttgcaattt gagcaaattt
    20701 ttttacttca tccgcatttt caacactaaa cgaaatcaaa gcaccacttg tttttttatt
    20761 atcagctatt tctttatctt ttataaattt tttataaaaa tcatgagaga gaagcattat
    20821 ccaaaaagtg tcatcccata ccattgaacc agcttcttca gtagaaaatt cttcattttt
    20881 tttaaaaccc agcttttcat aaaaagcaac ggaacgattg acatcagcaa caggaaaatt
    20941 aacaaaaacc atactattca tttgtttcgt ctcctttctg ttttgttcgt ctatcatctc
    21001 ttatttagga aacgctgtca aaaatttaga ttcatatata gaaaccaaaa taaaaatttg
    21061 catcatcaaa aaagtgaaga tgtgatagaa tgaggactgg ttcactttca cttaactaat
    21121 agatggaatt tctttataat aaaaaagtga atttatattg aaaaagaaaa aaacaagtaa
    21181 agccggggaa tattccggta tgaaaggaat ggagagaaca atgaccatcc aatggtttcc
    21241 gggacatatg gcaaaagccc gacgagaagt ttctgaaaaa atgaaatatg tggacatcgt
    21301 atttgaattg gtagacgcac gattaccttt atcttctcgt aatccaatgc tagatcagat
    21361 cattcagcaa aaacctcgcc ttgttttatt gaataaagcg gatctggcag atccacaaca
    21421 gacaaagctg tggcaagaat attttcgtaa acaaggccat cttgcattag ctatcacggc
    21481 acaagaaagt aaaggaatta aaggtctagt acctgcggct aaagaagcat tgaaagaaaa
    21541 acgggcaaga gatgctgctc gggggatcaa gcctcgcgct attcgggcaa tggtcttagg
    21601 tattccaaat gttgggaaat ctacgttgat gaatcgacta gtaggcaaga aaattgcaca
    21661 gacaggaaat aagcctggcg taacaaaagg tcagcagtgg cttcgtttag gtaatgagtt
    21721 ggaattgtta gacacaccag ggattttatg gcccaaattt gaagatgaag aaatcggcaa
    21781 aaaactagcg ctgacaggcg cgatcaaaga ccagcttgtc catttagatg atttagcgat
    21841 ttacggatta gacttttttg cccgatatta ttcaggaaga atcagcgatc gttatggttt
    21901 atcaatcgag caagaagaaa aattggcacc ggaattactg atggatatta cagcaaaacg
    21961 tgggtataaa gaagactatg atcgtggcag cgaattagtc gtgcatgata ttcgtcaagg
    22021 aaaacttgga cgatacacac tggatcgttg cgaagaaatg gagcaaaaaa gtgatgactg
    22081 aatccatcca aagtatcaaa gaacgattga aaagcataca gagaatagat gatcctacac
    22141 tagaatcatt aagaaatgat ccacgcaaag gcgtgcagca agcactggct tcttttgaac
    22201 gacgattgaa aaaggaagca ttaattaagg aaaaatatga agaaatgttt tgctttgaaa
    22261 agcaagggta cgcacaaggt caccaatgga ttgccggcat tgatgaagta ggaagaggac
    22321 cattagctgg acctgtcgtg gcagctgcag ttatcttgcc gaaaaatgag atcatattag
    22381 ggctgaatga ctccaaacaa ttatcagaga aaaagcgaga acttctttat caacaagttc
    22441 aagaaaaagc cctggcaatc gggatcggga tcgttgatca gcagacaatc gatgatgtga
    22501 atatttatga agcaagtaaa ttagcgatga tccaagcagt agaaaaattg tccattgtac
    22561 caagctattt gttgatcgat gcgatgacct tggatttgcc tattcagcag gaaaaaataa
    22621 tcaaagggga tgcacgatct gtgtcgattg ctgctgcaag tatcattgct aaagtatatc
    22681 gagaccaatt gatgaaagaa tatgacgtgc tttatcctgg atatggtttc tcgcataatg
    22741 caggttatgg aacaaaggaa cacttagaag gacttgaacg acaaggaatt acgccaatcc
    22801 atcgtaaaag ttttgcacct gtaaaaaaat atttacaata aaaaacagcc gcttttacgt
    22861 atcttattag aaagagagac gaaaggcggt tttatgatgt atcaaataga agaaaattta
    22921 ttgaaacttg cttatacaaa aggaatcagt agtcaaggaa aatgggcact tgctaactgg
    22981 atgatgcagt atccttataa agaaatcgga tttgaagaca tcattaagat aggtaaaata
    23041 accaaacacc gggagctgtt tcgcaaaagt tggcaggaaa ttcacgctaa ctggggcaaa
    23101 atccaatcga aacagtcgtt tatcacttgc tttgatccca aatatccccc tcagctgctt
    23161 cacttgacct atccgccaat tgtactgttt tataatggtg atctatcttt actttcttgt
    23221 aacatgttaa gcgttgttgg tggaagacac acttctgctt tagccaaaaa aacagtttat
    23281 tatttactaa aaccagtgat agaagctgga tatgtgatcg tcagtggctg cgcgaaaggg
    23341 atagatacat acggtcacca agcagcaatc tctcatggtg gacggacaat cgcagtcatt
    23401 ggaacaggga tagagcaagt ctatcctgct gaaaatcgtg acttgcaaga ggtgattgca
    23461 aatgatcatc ttcttttgag tgaatatcct cctcaatccg gcccgaaaaa gcattgtttt
    23521 ccaatgagaa atcggattat cgcagctttg tctaaaggaa catgtgtctt tgaagccaag
    23581 aaaaaaagcg gctctctgat aacagcacag caagcattgg atcttggaag aacagttttt
    23641 tctgttccag gaaatatttt aacagaacat tctaccggat gtcatcattt gatccaagac
    23701 ggtgcagttt gcacggtttc gggtgaagat atcttggcag aatttaaaga ataacaccat
    23761 acttttcatt tgcttttctt gacaaacgtt ttctgaatgg atatgattag ctacgatttg
    23821 tatgaatcct cttttaatag taggaacacg acaatggaaa ggagtccatt cagtaatggc
    23881 gtataaatat ttagtgattg tagaatcacc tgcaaaagcc aagacaattg agaaatatct
    23941 tggacgaaac tataaagtaa tggccagtgt tggccatata agagatttac cgaaaagtaa
    24001 aatgggtatc gattttgaaa acaattatga accccattat atttctatac gcggaaaagg
    24061 cgatgtcatc aaaagcctga aagccgcagc aaaaaaagct caaaaagttt acttggcaag
    24121 tgacccggat agagaaggag aagcgattgc ttggcattta gcgtacctac ttgggttgga
    24181 tctgaaagaa aaaaatcggg tggtcttcaa tgaaatcaca aaagacgcag tcaaagcagc
    24241 ttttaaggaa ccaagaacga tcgatgtaga tttagtagat gcacagcaag ctcgtcgtac
    24301 cttagacaga atcgttggtt attcgatcag tcctattctc tggcgtaagg tcaagaaagg
    24361 gttaagtgca ggacgtgtcc aatctgtcgc tttaaaaatt attattgacc gtgaaaaaga
    24421 gatccgagaa tttgttccag aagaatattg gagcatcgac ggtaatttta aaaaagctcg
    24481 caagaaattc aaagcaaatt tctggggaat cgacggtaag aaaaagaaat taccagatgc
    24541 acaaagtgta aaagaagtca ctgctagaat tgatggaaca gacttcgaag ttaaaaaagt
    24601 agaaaagaaa gagcgcaaaa gaaaccctgc gaatcctttc actacaagta gcctgcaaca
    24661 agaagctgcc cgtaagttaa actttcgaac aagaaaaacc atgatggtag cacagcaatt
    24721 atatgaagga atcactttgg gtaaacaagg aacagtcggc ttgattacat acatgcgtac
    24781 tgattctacc cggattgccg atacagcaaa agcagaggcc gcagcattta ttgaagatac
    24841 gtatggaaaa gaattttcta gacatgatca ccgaaagatc aaaaatgctc aaggcgcaca
    24901 agatgcccat gaagctgtgc gtccaacgag tgtgcttcgt acaccagatg aactcaaaca
    24961 atatttagat aaagatcaat tgaaacttta tacattgatc tggtcacgtt ttgtggctag
    25021 tcagatgaca ccagctattt tagatacaat gaaagtgaca ttagaacaaa atggtgtcat
    25081 cttcattgcg aacggttcaa aaatcaaatt caaaggattc atgcaagtgt atgtggaagg
    25141 tagagatgac ggcaaagaag agaaagaaaa tattcttcca gaacttgaag aaggcgatgt
    25201 agtccaatct gttgatattg aaccaaaaca acattttact caaccaccag cacgttttag
    25261 tgaggctacg ttgattcgtg cactagaaga aaacggggta gggcgacctt ccacttatgc
    25321 accaacactt gatacgatcc aaagacgcta ctatgtaaaa ttgacacaaa aaagatttga
    25381 gccgactgaa ctaggagaaa ttgtcaattc attaatctgt gaattcttcc ctcaaatcgt
    25441 cgatatccat ttcacagcag aaatggaagg agatttggat aaaattgaag aaggcacgga
    25501 agcgtgggtc aaagtggttg atcgtttcta taaaccgttt gaaaaagaac tgacgaatgc
    25561 ggaagaaaaa atcgaaaaaa ttcaaatcaa agatgaacca gcaggattcg attgtgatgt
    25621 ctgtggacat ccgatggtga taaaattagg gaaatatggg aagttttatg cttgcagtaa
    25681 ttttcctgat tgtcggaata cgaaaccaat cgtaaaagaa atcggtgtga cttgcccagt
    25741 atgtcatgaa ggacaagtca ttgaacgaaa atctaagaaa aacagactat tctatggttg
    25801 ttcccgtttc ccagaatgtg aattcacttc ttgggataaa ccagtgggca gaaattgtcc
    25861 aaaatgtgac ggatatctgg tcgagaagaa agtcaaaggc ggtaaacaag tagtctgtgt
    25921 aaatggagat tacgaagaag acatccaaaa ataatagaaa agaggttctt tcattgaaaa
    25981 tggtaaatgt tgttggagca gggcttgccg gaagtgaagc tgcttggcag atcgcacaag
    26041 caggagtccc tgtccgttta tatgaaatgc gtccagtcaa atcgactgag gcgcatcaca
    26101 caagtaattt tgctgaacta gtatgttcca attcattaag aggaaacagt ttggctaatg
    26161 cagtagggtt attgaaagaa gaaatgcgcc gattgaattc ggtagtaatc acttcggcag
    26221 atgaaacagc agtgccagct ggtggtgcct tagctgttga ccgcgattct ttttctgaaa
    26281 caattacccg aaaagtcaaa gagcatccat tagtaacagt gataaacgaa gaaattactg
    26341 aaataccgga aggaatcacc gtagtagcaa caggaccttt gacttctcat ccattagcag
    26401 aaagcatcaa agcattcaat ggttcagatg gtttttattt ctatgatgca gctgcaccaa
    26461 tcgtagacaa agctacgatc gatatggata aagtatattt gaagtcccgc tatgacaaag
    26521 gagaagcagc ttacttgaac tgtccgatga cagaagagga attcaaagct ttccatgagg
    26581 cactaatcaa tgccgaagtg gcagaactta aatcttttga gaaagaaaaa tattttgaag
    26641 gatgtatgcc aattgaagta atggcacagc gaggattcaa aacgatgctt ttcggaccta
    26701 tgaaaccagt tggattggaa gatcctaaaa ctggcaaacg tccttatgct gttatccagc
    26761 tgcgtcaaga caatgctgca gcttctttat acaacatcgt gggctttcag actcacttga
    26821 aatggggcga gcaaaaacgt gttttccgga tgattcctgg gttagaaaat gctgaattcg
    26881 ttcgctatgg tgtgatgcac cgaaacagct ttatgaattc gccagaattg ctgaaaccga
    26941 cttaccaatc aacgaaacgc gaagatctgt tctttgcagg tcagatgact ggtgtagaag
    27001 gctatgttga aagtgcagcg agcggactac ttgctggaat caacgccgca cgcttagcaa
    27061 aagaaatgga accagtcgag tttccacaag aaacagctat tggaagtatg gcttactaca
    27121 ttactcatgc ggaaggaaaa catttccaac cgatgaatgc caactatggt ctgtttcctg
    27181 aactgccaga gcgcattcga gacaaaaaag caagatatga agcaattgca caacgagctt
    27241 taaaagctaa tgaggcaata aaaacagaat tagaaaaaac tgcaatccca aatgagtaag
    27301 gaaactccat gaagagggtg agacggatag tctcgctctt tttcttgcgt ttttcttaag
    27361 aatttctcaa acaaaatgtg aggaattgcg ccaattgtga acaaacttag aaatattggt
    27421 taaattctga caatttattt gtctttttat aaacgctgtg ctaaattatt ctctgggaca
    27481 aaaggaaggt gagaaaatga cagaagccac gtgggaacaa aaatttttgc gttatttgat
    27541 tgtcgaaaga ggatattcag aaaaaacgaa agaagcatat gaagaagaca ttctccattt
    27601 caaacatttt ttggaggaat ctggagacgc tgatctgctg aaggcggaat attttacggt
    27661 tcgtgtctat ttgagtgcat tagctgatca tcgtcctagc tatagcagaa attctatcag
    27721 tcgcaagata gccagcctac gttcgtttta tcagtatctt ctcaaagaag aagtgataaa
    27781 agaaaatcca ttttcttacg ttcatctcaa aaagaaaaat ttgagactgc cacgtttttt
    27841 ttatgaaaat gaaatgcaag cactttttga cagcgtagca ggagatagtc ctttggattt
    27901 gaggaaccgc gctttactgg aagtccttta cgggtcaggc atccgtttaa gtgagtgtag
    27961 cagtttgtcg gtatcagaca ttgattttga ttcagaggtc atgttgatcc atggtaaagg
    28021 gaacaaagag agatatgctc ctctaggttc ttttgctcaa gatgctctgc aagaatattt
    28081 cagggaaggc cgccaggtat tgatgacaaa atatcacgaa gagcatgata tcgtttttgt
    28141 gaatcatcat ggaaaaccaa tcacgcctac tggaattgaa tatgtcttga atcaaatgat
    28201 caaaaaaagc agtttggata gtgatatcca tcctcacatg ctgcgccata catttgctac
    28261 tcatttatta aataatgggg cagatttgcg gacagtccaa gaattgctag gacatgccaa
    28321 tttatcgact acccagatct atgcccatgt aacaaaagaa agtttacaaa aaaactatcg
    28381 ttctttccat ccaagagcgt aaaaataagg aaaagaacaa atttaggagg aaacaaacat
    28441 ggtcgaatca ccatttcatt caaccactat ctgtgccgtc gaaaaagacg ggaaatttgc
    28501 tatggctgga gatggccaag taacaatggg tgaatctgtt gtcatgaaag gcactgcaaa
    28561 gaaagtacga cgtatctaca atggagaagt tgttgtaggt ttcgccggaa gtgttgcaga
    28621 tgcgtttact ttagaagaaa aatttgaagg caaactaaac gaatataacg ggaatctaca
    28681 acgcgcagct gtcgaattag ctcaagaatg gcggacacag caatctatgc aaaaacttga
    28741 agcgatgctg atcgtaatga atgataaaga aatgcttctt gtttcaggta caggagaagt
    28801 tatcacacca gatgatggta tcttagctat cggatcagga gggaattttg cgttggctgc
    28861 agcccgagca atgaaaaact atgctcaaaa cgacatgagt gcgaaagaaa ttgcagaaaa
    28921 tgcgttaaat attgccgcag atatctgtat ctttactaat cataatatta tcgtagaaga
    28981 actgtaagag gtgagtagat atgtatgaat taaaacaaac accaagacaa atcgtaagtg
    29041 aattagatga atatatcgta ggacagcacg cagcaaaaaa atctgttgcc gttgctttgc
    29101 gtaaccgcta tcgccgtttg cagttagatg aaaaaatgca acaagatatt acaccaaaaa
    29161 atatgctgat gatcggacct acaggggtcg gtaaaacaga aatcgcacga cgtttggcaa
    29221 aaatcgtcaa tgcaccattt gtcaaagtcg aagcaacaaa atttactgaa gtcggttatg
    29281 tcggaagaga tgtagaatca atggttcggg atttagtaga aaatgcgatc caaatcgtag
    29341 aaaaagagca gtacagccgt gtatacacaa gtgctaaaaa aaatgcagaa aaacgtttgg
    29401 taaaaatctt ggtacctggt atcaaaaaag aacagaaaca atccactaat caatttgaac
    29461 aaatgatgaa tctttttaat gctagtcagc aaaataccgc tgaaccgcaa gaagaagtga
    29521 cagaagatat tcgaatcaat cgccgtacag tcttggaaca actacaaaag ggtttactgg
    29581 atagtcgtga agtaaccatt gaagttgaag accaaaagaa acctgcacca atgatgaaca
    29641 atggattgga gcaaatgggg atcgacctaa atgaaactct aggtgctttg acaccgaaga
    29701 aaaaagtgga acggacggta acagtcaaag aagctttaga gttattgatc aaggaagaat
    29761 cagcaaaatt agtcaaggat gctgatatcc atagtgaggc aatcaaatta gcggaagaaa
    29821 gtggaattat ttttatcgat gaaatcgata agatcacttc taaatcacag caatctggag
    29881 aagtatccag agaaggcgtc caaagagaca tattgccaat tgtcgaaggt tcacaagtaa
    29941 acacaaaata cgggatcatc caaacagatc acgtcctatt cattgcttca ggagctttcc
    30001 atttgtctaa accaagtgat ctaattccag aacttcaagg acgcttcccg atacgggtag
    30061 aattagatga tttgacagct gaagattttg tgaagattct gaccgaacca aataatgctt
    30121 tagtaaaaca atacgtcgcg ttgatcggta cagaaaatgt gtcagttacg tttacaaaag
    30181 aagcaattga acggatcgct caaatcgctt tcgatgtcaa ccgtgacaca gataatatcg
    30241 gcgcacgcag actgcatacc attttggaaa aactactaga agatctatta tttgaagcac
    30301 cagatatgca aatgggagaa ataacgatca cagaagcata tgtggatgac aaacttggct
    30361 caattgcggc aaatgaagat ttaagtcgct atattttata aaaaatcgga ggacgacaat
    30421 gacagacttg ctaacgaaaa cccgcaagat aaacacactt ttgcagcaaa agaatacttt
    30481 tgacatgcaa gcagatttgc cttacgataa aatggctgtc acactaggtg gtattcttga
    30541 cagcaacacc tatatcatag gaagtgacgg agtattacta ggttataatg aacgtcacga
    30601 tgtgaacaat gaacgaatca aaacaatgtt tgttgagaaa caatttcctg aatcttatac
    30661 tgatacagtg gcacagttga tgaaaactga agctaatatc ccaattacta gtgatattac
    30721 tgcatttccg gtagagtcaa gagagaaata tccatttggt ctgacgactg tcgttccgat
    30781 ctttggagct ggcgaacgtt tgggtacgat tatcttatct cgtatggaac aatcgtttgc
    30841 agatgacgat ctagtcttag cagaatatgg agctacggtt gtcggtatgc agatccttta
    30901 tcagaagtct cgaagcatcg aagcagatgt acggagtgca acggctgtcc aaatggcaat
    30961 caacacatta tcttatagcg aattgaaggc tgtacaagct atctttgaag cattggatgg
    31021 agatgaaggt cgtctgactg cttctaatat tgccgatagt atcggcatca ctcgttctgt
    31081 gatcgtcaat gctttacgaa aattggaatc agctggaatc attgaatctc gttcgttagg
    31141 catgaaggga acttatttaa aagtattgaa ttcccgtttt aaagacgagt tagcaaaaca
    31201 cagttattag aagtaagggg actggggcag atagaaatgt ttgttccagc cttcccattt
    31261 taaaaagtaa gtcgaagttg gaggaatggc aatgactgtt attattcaaa atgaccagct
    31321 gatcgccgaa atcgctgaac acggtgcgga attgatcagt ctgaaatcaa aagaacatgg
    31381 tttagagtat atctggcaag gtgatccagc gtattggggc agacatgcac cggttttatt
    31441 cccttttgtt ggacgtttaa aagatgatca gtatgtttat caagggaaaa catatcaaat
    31501 ggggcaacac ggatttgctc gggatatgga ttttgaagtc attgaacatg gacaagaaat
    31561 ggctagtttc atcttaagga gtacgccgga gacattagaa aagtatccat ttgattttga
    31621 attagtgatc agctatgaat taggaggaga cggcatcacg gttcattatc aagtggcaaa
    31681 taccggtgaa aaagaaatgt tcttttctat cggtggacat cctgctttca atgtacctct
    31741 tgaagaagga ttgtcatttg aagatttcta tctggcattt tctccattga aatcaagatt
    31801 acgcctacca ttgaaaggcc cattcatcga tatggatcaa aagacgttag gtcagacaaa
    31861 tacgaatctg gctttgaatc gtgaactttt tgagcaagat gccttggttt ttgaaacaag
    31921 gggtctaaat gcgtacgcca ttcgaagtga aaaatcttcc catagcgtaa ctttaagcta
    31981 taaagatatg ccttatgtag ggatctggtc tccttatcca aaagaagcgc ctttcgtttg
    32041 tatcgaaccg tggtgcggaa tcgctgacac ggtgaacagt agtggaaact tgatggaaaa
    32101 agtagggatc aatcaactag gtgcccatga aatcttcaag acaaaatatt ccatcacagt
    32161 gaagtaagag gttgtgaccg agaagttagc agggaacacc gtttaccaag gtgtgtaaaa
    32221 aagctatcct gtaagtaata agggagtgtg acatcgattt gtcacactcc cgttttatct
    32281 ctttggaatg aaaaagtatc aggatttttt cttgctgcta ttcaaaccaa acgggattcg
    32341 actttcagta tggtttctga tacgcttgat attgtcttta tgccgataaa agataaagga
    32401 agaaactaaa aaggcaatga tcgtcagtaa ccaattgggc ttagccaaaa tcacaggagc
    32461 tacataagga aggacgatcg tagataaagt gatcaatact gcactgatca tactgctcaa
    32521 actcaccata cttgtacaaa ataaactaac gataaagatg atacaagaat aaacaaaaaa
    32581 tacaggatta aatcctaaaa gcattcctgc acttgtcgca acagcttttc ctcctttgaa
    32641 cccagcaaaa atcggaaatg tatggccaag aatggcgcat accccaaacc aaagagggtt
    32701 aatcccagaa aggtggaaga tcatcggcaa acaagtagcc aatgtacctt ttaaaatatc
    32761 cataagcagg acaacaatcc cggctgtttt gcctaataca cgaaatgtat tggtcgttcc
    32821 agtattaccg ctcccgtgtt gtcggatatc ttttttgaaa aacaatttgc caatccagac
    32881 accggaagga atagaaccta aaagatatgc taaaataagt aataagatca atttcatttt
    32941 ttaccctctt tcaagttgaa aactatcctt tattttagca tgaataggta acaggaacaa
    33001 ttgaaaaaat gaaagataca agtataatag atggaataaa aagaaaagca aggggtggca
    33061 gaaatgtctg taaaaaatcc aacacaagaa acgattttca actatttgga agaagcaaaa
    33121 aatattgccg tcgtaggctt gagcaataaa ccagaacgaa caagttacat ggttgcacag
    33181 gtattgcaag acaatggata taaaatcata ccagtaaatc cattattggc tggtgaagaa
    33241 attctaggcg agaaagtcta tgcgaatttg acagaaatcg atgatccaat cgatatcgta
    33301 gatgtattta gacggagtga atttttacct gaaatagcag gtgatttttt gaaaactgat
    33361 gctaaagtgt tctgggcaca attagggatt gaaaacaaag aagcagctga gatgctaaga
    33421 aaagcaggac gtacagatat cgtaatggat cgttgtatta agattgaact tggtaagttg
    33481 aaagaaaacc aaaaataaaa atgaccacaa agaagagatg atcgatagag gggaatgtcg
    33541 aaaaagcggt taggagataa ttaaaattga tttctcgtct accctctttt ttttcacgac
    33601 cttttctagt atgataatcg tggtgcggtt ttgccgcaaa gtattattga aggagcgttt
    33661 gccttggcaa aaaaagtaaa caacgaatac aatgactcgt ccatccaagt attggaagga
    33721 ttagaggcag ttagaaaaag accagggatg tacattggat ccactgacag ccgaggcttg
    33781 catcatttag tctatgaaat cgtcgataat gcagttgacg aagcattatc aggttatggc
    33841 agtgagattg atgtgaccat ccatgaagac aatagtataa ctgttgcaga tagcggacgt
    33901 gggatgcctg taggaatgca tgcttccgga ataccaacag ttgaagtgat ttttactgtg
    33961 ctgcatgctg gcggaaagtt tggtcaagga gggtacaaaa cttctggtgg actgcacggt
    34021 gtaggagcca gtgtggtaaa tgccttatcg aaatggctaa ttgtaacgat cgttagagat
    34081 ggcgtagagt atcagcagaa atttaaaaac ggcggtaaac ctgacggtac tttgaaaaag
    34141 ataggaaaaa caaaaaaagc aaacgggacg actgtccatt ttttaccaga tgatacaata
    34201 ttcagtacaa cgaaattttc atatgaaatt ttagccgaac ggttaagaga atctgccttt
    34261 cttctaaaag gcgttaaaat ctctctttct gatcttcgtg gagaagaacc agtcaaggaa
    34321 atttttcatt acgaagaagg aatcaaagaa tttgttgatt atctgaatga agaaaaagat
    34381 acattgacac cagttgttta tttttcagga gaaaaagaag gtatcgaagt agaggtcgct
    34441 tatcaataca atgacggtta ttcagaaaat gttttatctt ttgtcaataa cgttcggaca
    34501 aaagatgggg gaacccatga agctggtatg aaagcggcga tgacgaaatc ttataatgaa
    34561 tatgcgagaa aagttggttt gctgaaagaa cgggacaaga atctggaagg tagtgatttc
    34621 agagaagggt tagctgctgt actttctatc cgtgtacctg aaaatcttct gcaatttgaa
    34681 gggcaaacaa aagaaaaact tggtacgcca gttgctcgta ccgtagtaga taacgtcatc
    34741 agtgaacaaa tgggctttta tttgcaggaa aatagcgaaa tgagccagat gttggtccgt
    34801 aaagcaatca aagccagaga agcgagagaa gcagcacgta aagccagaga agaaagccga
    34861 aatgggaaga agcgtaaaaa aggcgaatct cttctttcag gaaaattgac acccgcacaa
    34921 tcccgaaacc ctaagaaaaa tgaattgtat ttagtcgaag gggattctgc tggcggatct
    34981 gctaaacaag gaagagacag aaagttccaa gcaattctac ctttacgagg aaaagtcatc
    35041 aataccgaaa aagctaaaat gcaagatatc ttgaaaaatg aagaaatcaa tacgatgatc
    35101 tatacgattg gtgcaggagt cggtcctgag ttttctatcg aagactgtaa ttacgacaaa
    35161 gtaatcatca tgaccgatgc ggatacagac ggcgcccata ttcaagtatt actgcttact
    35221 ttcttttatc gttatatgaa accgctgatt gaagctggga aagtatatat cgcgttaccg
    35281 cctttatata aagtatccaa aggacaagga aagaagcaag tcattgaata tgcgtggaca
    35341 gatgacgaat tggcagcaat gattaaaaaa gtcggcaaag gatatatgct gcagcgatat
    35401 aaaggacttg gagagatgaa tgcggaacaa ttgtgggaaa caacgatgga tccgacttcc
    35461 cgtacgttga tccgcgtccg tatcgatgat gctgcgcaag ctgaacgtcg agtaaccaca
    35521 ctgatgggtg acaaagtaga acctcgaaga aaatggatcg aaaatcacgt gcaatttacg
    35581 ctcgaagaag atggcagtat cttagataaa aaagaggata ctgaaatctc tccttcagtt
    35641 tctaatgatt tattagatga agaacgagca gataaaaatg aaaacaatca gttatttgaa
    35701 gtagaatagg aggtcacctg atggaaaatc gacaagaagt acaagaatta actttagaag
    35761 aagtgatggg tgaccgcttc ggacgttatt ccaaatatat tatccaagaa cgtgcactgc
    35821 ctgatatccg tgatggattg aagccagtgc aacgccggat attatttgcc atgaacaaag
    35881 atggcaatac gtatgacaaa ggattccgta aatcagcaaa atctgtcgga aatatcatgg
    35941 ggaattatca tcctcatggt gacatcagta tttatgaagc gatggtccgc atgagccaag
    36001 actggaagtt gcgggaagta ctgatcgaaa tgcacgggaa taacgggagt atggacggtg
    36061 atccacctgc tgcgatgcgt tatactgaag ccagattatc caagttaagt ggagagatgc
    36121 tagcagatat cgagaaagaa acagtcgatc tcgtgtggaa ttttgatgat acagaaaaag
    36181 aaccaacagt ccttccagct cgatacccaa accttctcgt caatggatcg acaggtatat
    36241 ctgctggata tgcaacagaa atcccgactc ataacttagc tgaagtgatc gatggcacaa
    36301 tctatatgat cgatcatcca caagcgagcc ttgaaaaatt gatggaatat attcctggac
    36361 ctgactttcc aacaggaggg atcttgcaag gaaaagatga aatcaaaaaa gcttatgaaa
    36421 caggacgagg aaaagttatt cttcgttcga aaacaaagat agaggcaatg aagggtaata
    36481 aacagcaaat cgtgatcaca gaaatcccat atgaagtaaa taaagcgacc ttggtgaaaa
    36541 aaatggatga gatccgtttg aataagaaaa tcgatggaat agctgaagta cgagatgaaa
    36601 gtgatagaac aggtctgcaa attgtcgtcg aattgaaaaa agacgccaat gctcaaggaa
    36661 ttttgaatta tttgttcaaa aacactgagc tacagatcaa ttacaatttc aatatggtcg
    36721 ctatcgacca tatgactccg catcaagttg gactgaaaga cattttaaga agttatatcg
    36781 agcatcgaaa gcaagtcata acaaaacgta gtcaattcga tttagccaaa gctcaaaaaa
    36841 gacagcatat cgtagaagga ttgatgaaag ctttatcgat tctggatgaa gtaatcgcaa
    36901 cgattcgtga aagtaaagac aaaaaagacg ccaaaaagaa tttagtcgac gtctttcaat
    36961 ttacagaaga acaagcagaa gcaattgtca cattgcaatt ataccgtttg acgaacacgg
    37021 atatcacaga gcttcaaaag gaatccgaaa gcttgattgc gcaaatcaca gagttgaata
    37081 aaatcctttc aaatgacaaa gagcttttct ctgttatgaa aaaagaattg cgagaagtga
    37141 aaaaaaatta ttcttccgct cgtctaacga caatcgaaga cgaaatcgaa gaaatcaaaa
    37201 ttgatacgca agtcttagtg gcacaagaag acgtaattgt cagtgtgacg cgagaaggct
    37261 atgtgaagcg aacaagcctt cgttcttata gtgcttccaa accagaagaa atcgggatgc
    37321 gagaaggcga ctacttgcta tactcaggtg aattgagtac attggaccat gttcttctga
    37381 tcacaaataa agcaaatgtg atctaccgcc ctgtccatga actacccgat ttgaaatgga
    37441 aagatgctgg tgagcacatt tctcaaacaa tctccaatct gtctgtagac gaatcgattt
    37501 tagctgtttt cccatatcaa aaaattcaag cagagaaaac atttgtattc atcagcaaga
    37561 atggattgat caagcaaaca cgaatgacag agttcgaacc atggcgcacc tataagagcc
    37621 gtccgctcag ctgtatgaag ctaaaggcgg aagaagatga actgattgct gtgtatctga
    37681 aagaagatca gccaggctta gatgttttct tagtaacgca tcaaggaatg ggtttgcgct
    37741 atcctttaac agatgttccc gttgttggag caaaggcagc tggagtgaag tctatgaatt
    37801 tgaaagatgg agataggatt atcaacgggc tgcttgtgtt agcagaaggt gatacgccga
    37861 tcgtaatcgt tactcagcga ggagctgtta aacgcatgct tgctcaagaa atcagccaaa
    37921 cttcacgtgc gaagcgggga gtgacggtca tgcgtgagct gaaaaaaaat ccgcaccgta
    37981 ttatctatat gtcggaagga agatcaaaag aaataacctt gatgaatcaa aaagggcagc
    38041 agttaacgat tgacccaact gatttcccta taggtgatcg aacatcgaat ggatcatttg
    38101 cgatggatga gaaaaaaggt ggagaagtca tcgaagttat cgatgctcct gagattttca
    38161 tcgagtcgta actgttgtag tattttaatg aagagacgcc atctgtatta atacaggtgg
    38221 cgtctctttt tcattcattt ttaataaatc cttatacgtt tgcaaaaaaa gatgaaagtg
    38281 ttcacaatgc ttaaatgttg gtatgtcaac gttgatttaa atatatgtaa ataaaacata
    38341 aaaattgtta aataaaaaac aagaaaaccc ttgcaaagat agggtgctgt gttatactaa
    38401 tcacgtaaaa actgttacat aaaacagaaa tttaggaggt ccatacaatg gagcaatgga
    38461 atggatttaa aggtaccaaa tggaaagaag gcgtagacac tagagacttt attcaaaata
    38521 actacactga atatcgcggt gatgacagct ttttggaagg cccaacagaa gcaacagaaa
    38581 aattatggga tgaattgcaa aagttaaatg atatccaatt cgagcgtaac ggtgtctatg
    38641 atatggacac agaaatcgta tcaactatta cttcacacga tgcaggctat ttggataaag
    38701 atttggaaaa aatcgtcggt ctacagacag acaaaccttt gaaacaagca ttcatgccgt
    38761 tcggcggaat caatatggct aacaatgcgc taaaaagcaa tggctataca ccaaacgatg
    38821 aattgacaca tattttcact gactggcgca aaacacataa tcaaggtgta tttgacgctt
    38881 atactgctga aatgcgtgct gctcgtaaaa acaaaatcat tacagggctt ccagacgctt
    38941 acggtcgcgg acgtatcatc ggtgactatc gtcgtgtggc attgtatgga atcgactatt
    39001 tgatggaaca aaagaaaaaa gatcatgata tgactggtta tcatacaatg agtgaagaag
    39061 tgattcgttt acgtgaagaa atcactgaac aatatcgtgc attagcgcaa atgaaagaaa
    39121 tggctgcgaa atatggctat gatatttcta aaccagctgc aaatgcaaaa gaagctgtcc
    39181 aatggctata cttcggatat ttagcagcaa tcaaatcaca aaatggtgct gcgatgtcaa
    39241 tcggacgtat ctcagcattc cttgatatct atatccaacg tgatttggat aatggagtaa
    39301 tcacagaaaa agaagcacaa gaattgatcg accacttagt aatgaaatta cgtatggtca
    39361 aattcgcgcg tacacctgaa tataaccaat tattctcagg attccctatc tgggctactt
    39421 tatccattgc aggtatggga ttagatggtc gttcattagt tacaaagaat gatttccgtc
    39481 tgcttcatac attaacaaat atgggacctt caccagaacc aaacttgact gttttgtatt
    39541 ctgaacacct tccagaagga ttcagaacat atgctagcag aatcgctatc gaaagttctt
    39601 cgattcaatt cgaaaatgat aatcagcttc gtgaacaatg gggttctgat gattgtgcga
    39661 ttgcttgctg tgtatcagga acagttgtcg gaaaagacat gcaattcttt ggcgctcgtg
    39721 ctaacttggc taaaacacta ttatatgcca tcaatggcgg agtcgatgaa gtgactggcg
    39781 cccaagttgg accagaatat cgtccaatca catcagaaat actagacttt gaagaagtga
    39841 aacataactt catgaatatg atggattggt tagcagaact atatgtcaat acgttgaaca
    39901 tcattcatta tatgcatgat aaatattctt atgaagcagc tcagctggct ttgatggatt
    39961 ctgacttgca acgcacattt gctacaggta ttgctgggat ctctcatgcg gcagacagct
    40021 tgtcagcgat caaatatgca aaagtaaaag caatccgtaa tgaaaaagga atagcagttg
    40081 attttgaagt ggatggcgac ttccctaaat acggaaatga tgatgaccgt gcagatgaat
    40141 tagtttcatg gatcttggaa tacttcatgg gacaaatcaa acgtcataaa acttaccgca
    40201 actcaaaacc aacaacttca ttattgacga ttacttcaaa tgttgtttac ggtaaaaata
    40261 caggaaatac tcctgatggc cgtgaagctg gaaaaccact tgcgccagga gcaaatccaa
    40321 gttacaatgc ggaacaaagt gggttattag ctagcctgaa ctcaacagct aaaatgaatt
    40381 acggctgtgc attggatgga atttcaaata cccaaacaat caatccttca ggtcttggaa
    40441 aagaacaaga tacaagaatc gaaaacttgc gtaatgtctt agatggatac ttctcaaaag
    40501 gagcttatca tttgaatgtc aatgtattca gtaatgattt attgcgtgat gcaatggaaa
    40561 atccaatgaa atatccgaac ttgacgatcc gggtatctgg ttatgcagtt aaattccgtg
    40621 acttgacaag agaacaacaa ttagatgtct tgatgagaac ttcacacgac agaatgtaaa
    40681 aagttcgtga aaaaagtttt tcgacttagc gtcgaaagga cgttgaatag ccatcgcatc
    40741 aaaatcgaat aatcagtatg cttatttgaa agaaacgatt tacaggggca ggacttgtat
    40801 gttccgtccc tgtaaatttt acacataacg aagcggcaat tgcgaaaaat tttgttcaga
    40861 aagcttcgct tcgacagcta acttgtactt aaaatgaaat caaagaaaag atgtgaactt
    40921 aaatggaaga aaaaacaatc ggctatgttc attctatcga aactttcggt tcagtagacg
    40981 gacctggact gcgctttgtc gtgtttatgc aaggatgccg catgcgttgc caattttgtc
    41041 acaatccaga tacttggaat attggcggag ggaaagaata cacagcagat gaacttttag
    41101 ataaagcaga acgcttcaga ccttactggg gagataaagg tggtattact gttagtggtg
    41161 gagaacctct attgcagatc gattttttga tcgaactatt taaaaaagca aaagagcgga
    41221 agatgcatac tacattggat acatgcggaa aaccttttac gtatgaagaa cctttctttt
    41281 cacgttttca agaattgatg aagtatacag atttactgtt atttgacatc aaacacatcg
    41341 ataatgaaga gcataaaaaa ctaacgcatt gggacaatga aaatatttta gaaatggctc
    41401 aatatttgtc aaaaatcaac aaaccggttt ggattcgcca tgtactagtt cctgagcgaa
    41461 gtgattatga tgaatatttg atccgactag acaactttat tcaaaccttg tcaaatgtag
    41521 acagagtaga gattcttcct tatcatacga tgggaaaata caagtgggaa acacttggtt
    41581 tgaaatatcc attagaaggg atcgaaccac ctacaaaaga acgtgtcgaa aatgctaaac
    41641 gattgcttca tacagaagag tatacaaaat atttaacgcg ataggtcgca tcggaaagaa
    41701 gataagtatt gcttctttcc gtttttttgc tattattaaa taggagaaat cacaaaggag
    41761 cgaatgacat gtcaaaaatt ttagtttttg gtcaccaaaa tccagataca gatgcaatcg
    41821 gggcagccat tgcgttttca cacttgcaaa atgaattagg taaagaaaca gaagcagttg
    41881 ctctaggtac acctaatgaa gaaacacaat acgcattaga ccactttcag ctatctgcac
    41941 cacgagtagt agagtcagct aaagcagaaa atgcagatgt catgcttgtg gatcataatg
    42001 aattccaaca aagtatttca gatatcagcg aagtaaatat tttagctgta gtagaccacc
    42061 atcgtattgc aaactttgaa acagctaatc cattgtacta tcgtgcagaa ccagttggat
    42121 gtacaagtac gattatttta aaactatata aagaaaacca agtaacaatt cctaaagaaa
    42181 tcgcaggaat catgctctca gctatcattt cagatacatt gttgttcaaa tcaccaacat
    42241 gtacgcctga agatgaagca gctgcaaaag aacttgcagc aatcgctgaa gtcgatctaa
    42301 acacttatgg acttgaaatg ttgaaagcag gaacaaatct aagtgataaa tcagctgcgg
    42361 ttcttcttga tttggatgca aaaagtttcc caatgggcag ttccaacgtt cgtatcgctc
    42421 aagtcaatac agtggacctt aatgaagtga tggatcgaca agcagagcta gaagcagcaa
    42481 tggaagatga aatgaaagca aatggctacg atctgttcgt attagttgtc acaaacatct
    42541 tagacagtga ttcagaatta cttgttgctg gtaaaggaaa agaaaaagta gaagaagctt
    42601 tccaaacaac tttgaccaac aaccgtgctt tgcttaaagg tgtcgtttca cgtaaaaaac
    42661 aagtcgtacc tcaattgacg gctgtattta gctaatcaaa tggtagtatg aagcattcaa
    42721 aatgaatgga taagcgattg atataaaaag aaacatagaa catactaaag gccgaagaaa
    42781 tggaggagaa taacagtcaa ttatgactct tgttcttcct cctttttacg taaaagcctt
    42841 ttaagtaaaa taaaacggat agaaaagaga acatatatga actattattc aaaaaataag
    42901 gaaaactttt atcaaaaact gaccgatgat ccgctttttt cttcattgac cgattatcta
    42961 tatgaacatc gagaacaaga aacgattcta agagaattga aaaaagaatt ttcgcaaaat
    43021 aaattttccc attttcttga tttattgatc gatgcaggat taatcaagag ggaagaacga
    43081 agatatcatt tgaactttcc aatatttgat cctaaagatt acttgcaaca agccacttcg
    43141 gctgctgaaa ccatagctga acaattgaag agactctctg ttgatgaaca aaaattagcg
    43201 atgggagaag ttatttgggc gtattgtttc gaagatgaac ggaaagaagc gtatttttat
    43261 ggagtaagaa acagccgaga gacagaattg ctgaggacaa ctgcaggaaa tgagaagtac
    43321 cgttttatca cactttcctc tatagaacac ttcccgctta ctttggctaa ttactttttt
    43381 gtacagaaga atcagcttcc tgtaacgaag gcattcaaag aattagccga acttattgga
    43441 gacgtaaatg aatcgtactt ttttgatcaa atagaagtta tcgttgacag gatacgtaag
    43501 aataaatata aaaatcgccg gcctagtatt ttccatcagt ctctacttgt gacgaatact
    43561 ataaaggaag aagaatcatt cacattagaa ttacctattg tcgaaaaaaa caattttgag
    43621 attgaattac caacattgga tccttcattg acaatggagg aaatggcctt tcttaaacga
    43681 caaatctttt ctgagctgag caagaaattt ataccgcatg cattttctta tatcaaagaa
    43741 tatggaacag ttttagtgag taaaacctaa gagagcagta agcaaaaatt atactgactc
    43801 ttgcaggagg agcctttctc ttcctttatg gttaaaagaa ttatttgagt attttgccta
    43861 tctaccacct attagattca ctacatggtc tggaaacaaa ggaatgcaaa aaaatggtga
    43921 gaaatgtgga atgacaaagg aagtagtgat aagaaaagtt agatttttaa ataatcaata
    43981 ttatgattca gtcaagtatg gcattttatg ggaagaacta gctgattaga ttaggaagtc
    44041 catttgataa tccagaatca ctaagaaacg aatggcgagt cattaataat ttatccaatc
    44101 ttgggttatt cttttagatt gaagtgttta aaactaaaaa agcatttatg aagtcgtaat
    44161 agtcaggctt caaaatgctt tttattttgt ttgttttttc tttgccacat aaatacacct
    44221 agactgacga tgaagagcat atccaacaga tttcctgcga cttggatcgt cgaagggttg
    44281 gtcagtggca ttaaagcaag gacaaaaaag gaaaacaagc tgtaaataaa acaggtgaca
    44341 ggaactaatc gaaagaagag cggacatttc aattggctaa aaatgagtga aaaaataaaa
    44401 taagtaaaaa tggataaaat cacgactaaa ccattcatga taaagaagaa aagcgtattg
    44461 tgattaaacg aataaaaaag aattgctaaa aaagaaaacg ttttaaatgc aatcaatacg
    44521 cttgaagctt cgtttaacgt accaggatgt cgatgaaaaa aataggcacc gaaataaaca
    44581 gaaaacagta agccgcaaag agaagacaaa atcaaaaaaa acggattttc ttcggcttct
    44641 gtgatatcag aatgatccaa aagcaaccag cctaatgcac tagaaggcgc atgtgaggtc
    44701 ttaaggtatg aataacatag aaaaaataag atcgtcaaag ttgcaaaaaa agagcctaaa
    44761 tgtaaaaaat tagtggttct acgttgaatt tccataatat ctccaaatta aattgataag
    44821 tatataaagt ataataacaa attgatgagt atatgtcgat gttttttaat caaaataata
    44881 aataaaaact gtctaacatt taaaatttaa tttagatatt agacagtttt taagtttgtg
    44941 agttattttc cttgtcgttt tctcactttt ttagtccaga aaccacctga aatatatttt
    45001 ggctcataag aaatgataaa agcacgctct tcgatttctt tgatcaggcg atacagttca
    45061 cgttcgcttt ttctaggaga aagtatttcg agaactagcc gctcaccttc cagccccatt
    45121 gcagaagtct gtgtcacacc ataaccgtgt tctctcagta tacccggcaa acttttatct
    45181 tctgcgctat ttgctggcaa aatcgttgtg accatgatgt atcctaatgc caatttgtct
    45241 tcaatcaaaa ttccgacact gatacctact gcgtaaccta acgcatagac aaataaattc
    45301 agtggattat ctaaacgatc taaaacaagg gataagccga cgatataaat cgtgatctcg
    45361 accatactga caagtggagc aatcagtcga tagcctttca ttgtcaacat gaaacgtaac
    45421 gtgttcagag tgatataagc aaaattaatg aggaagatca tccccaacat ttttggatca
    45481 atcatttgta aaactccttt catttatata acaacaatat caaaaaaaat cgtaagtgaa
    45541 gaaaaattga aactttatac atacaaaaaa atgataaaat gattataata gaattataaa
    45601 gataggttag gtgattaaaa tgaaaacggt caatcgtgtg ttttgtgcca gccacgtgct
    45661 aagtctcctt ctcagtatat acttaggtgt ctattcgatt caacagccaa ttgaggtaga
    45721 gaacattgtt tttcagatgt cgctgacaga tggtttaatt ttaagcgttt ttttcttttt
    45781 gatactgttt attggtaata ttttcggtag cgttgccagt tttttgaatt tttctattta
    45841 tccactcctt tcgctagcat taggagtggt gggactttgt tctctgtgct tatttcccga
    45901 gccattcagt cctgcattta tattatttgg tacactgaac ctgttccaga caactgttgg
    45961 agcatggttg ctatggcgat caggaaattt aatgaaaata ggtgagtaac aattgaatcc
    46021 aattatattg aaacgttctg tccatcatat tccagtgtta gaagttgttc tgaaagaatt
    46081 aagagaggaa cgtattcccg ttgtcatcta ttatcacgga tggcagacaa gtaaggagtt
    46141 agttttgacg caaggaagaa aaattgcgca gcagggcatt cgtgtgatcc tgccagatgc
    46201 gatgaatcat ggggagcgca aacagccggt ttcttctatt ccatcctata cattttggga
    46261 tagtatctac ggaaatctgt ttgaatttga cacacttatc aagcatttag aaaaaagagg
    46321 tcttctacat gatcagcttg cagttggcgg ggtttcgatg gggggaatga caacatgcgc
    46381 cttgttggct aaacatcctc atatagtagc gggcgtgtgt ttgatgggtt cccctgcacc
    46441 tttggaatat ggaaaagaaa tcgtaaggcg cgcatcggaa tataattatg tcgtaccaaa
    46501 agactatttt gatttgattt catgggttaa gagctatgat ttgtcattgc agccggaaaa
    46561 gttagctgga cgtcctctgt tcttttggca tggacaagct gacgaaaaaa tcccttatat
    46621 acaaacggaa cgatttgtag aaaaaaatcg gcataagtct tacggaaaag aaattgtttt
    46681 tcgttcctca aaaacacagc gacatatggt acaaatacca ctaatggaag aagcttccac
    46741 ttttttagca aaaaatttga taaaaaagta taaataattg aaacggaaaa tcactcatga
    46801 cgatagagga ttttccgttt tttattttgg gctataataa actctgttag gaaaatttgg
    46861 tataattgat agtaagaatg acaacatgtt agaaaggaag agagcatgcc cactttagca
    46921 gatgttgcaa aacgtgccaa tgtttcaaaa atgacggtct ctcgagtcat caatcatccg
    46981 gaacaggtga ctgacgaatt gaaagagtta gttttctcag caatggctga actggattac
    47041 cgtccaaata ttgctgcaaa agcacttgtc acgaatcggt cgcagatcat caaattattt
    47101 atactggaag aaatcgacac aactgagccc tattacatga atttgctgat gggaatcgct
    47161 aaacggatcg gtaaagcgca ttattctctc caattagtta caaatgatgc cttcgacgtc
    47221 ggttcttgtg atggatacat catcacaggt gtcagaaaaa aagacttcga ttggatcgac
    47281 cgtttggaaa aaccagttgt cttgtttggg gaaaattctg aaggatatga ttatgtagac
    47341 agcgataatg aatatggtac agcaaaagca acgcaatatg ccttagaaag agattatcaa
    47401 acgattattt atatcggtat cgaagtggac gagccttttg aaatttcaag ggaaactggt
    47461 tatttatctg tgatgaagga agttgatctt cctagtaaaa tcttgcggtt tccaaatcat
    47521 tcgacagaag tagaaagata tatgacagaa aactgggcag attttacaga taaaacttgt
    47581 tttgtatgta gttcagatcg gctagcacta ggagcagtaa gagggattat aaatgctgga
    47641 ggacacttgc ccgaagaatt tggtgtgatt ggatttgatg gggtattttt agaccaaatc
    47701 agttcaccta aattgacaac gatcaagcaa gatattatac gtatgggtga agtatgtgga
    47761 gaaatgttgt taaaaaaaat agaagaaaaa ggggcgagtc aaggttatcg tcgttttttt
    47821 cctgaactag ttatcagaga gacaacgaat taagtcttat tactatatat atatttatga
    47881 aattcatcgc tattttctta aaataatcag cgcattgcta ctgaaaacaa tgaatctgtg
    47941 ctattattga gaaaagttcc atttttgaga aggatggttt gaatgaaaga aatcagacga
    48001 aaacatttcc gttttcctgc gtacgatgat gagttaggcg taaaattaac caacgacaag
    48061 aagcgagtat tatttgacgg acaagaagat cttttgactg aacagaatca gtccccgttt
    48121 gaacagatga aagaatttca tttcacgaac acgaacaccg agaaaatccg tcgcaagcgt
    48181 cgtgcaacaa atggattgaa gtcagctccg cgtaaggaaa atttggaaaa gcataaagga
    48241 aaattaccgg attataccgg ccattaccaa tcagaaatca catcgacagg acgaaaaaaa
    48301 ctttttggtg attcctctcg ttctgtcaga caaagtgaaa caaggacaga gtccaagcat
    48361 actacacgaa acgcatctgt tgacacgaag gtgcgggagc gttcttattt tgttcctaaa
    48421 tatgtacccg catcgattat cccagaccct agagaaagca aaatttctga ggatcattta
    48481 atcgaatcga tgaaaaaaac ggatgactct tatctgttgt tcgatacaga accgacgccg
    48541 tatcaagaaa aaagaaatgg tgagccgtct gtacgaacat tcaatcgaac aaaaacgtta
    48601 gaggaaacaa agcagtcaga agaaaaaaaa tcgaaaaaaa gccatggtat cttagaacgc
    48661 tcattacaag gattgatcga tgatcaagga aactctttgg gagaaaatag ctattttaaa
    48721 taagtgatat acaaatttat attcggctac agttggaaag gaaacgctgc ggcgtttcct
    48781 tattttatac tctatccgaa agtttatatc gtatcctcaa atttttttga gtttaagcga
    48841 attttttctc ccaataagta agaaaataat ggcaaaataa aaaaacaaac tattttcgca
    48901 gatcgtttgc aaaaatagtt tattttttta taaagaagat tttactgaat aatatcattt
    48961 tagtttacat aatgatattt ctgtaaatta aataaagtag tgaaaaataa gaatcagtgc
    49021 tctaactgtt caagaggatc aagtggttca aatgtttctg tagtctgttt tgcttccaat
    49081 tggggcaaaa cagatccaat ttccagatac ttcactttat tcaatgtata agatagaata
    49141 gaacagctaa gtaatgtata cagaaaatgt gtgagatcca aataaagcaa ggagacaact
    49201 aaaataccaa aagtaatcaa gaaaagactt ttgccaatct taatatgaaa atatttttca
    49261 atggcttgat aaatgatatc tgttccgcct gtgcttccgc cgaatcgaaa gacgagaccg
    49321 caaccaatgc cagaaagaat acctgtcatc aaactattga tcagtagact atctacagca
    49381 aaatgtatat gaaggctctc ccataaatgt aaaaagaaaa ctagcgcaaa tgttccgacg
    49441 atcgtccgga gaaaagcgtg ttttccaaaa agaaacaatg agataagcaa taaaggtaaa
    49501 ttaaaaagca gtgaagtgac agaaggagcg aatccaaaaa gattatgcaa aagcaaagca
    49561 ataccagtaa ccccaccatc tgccagctta gatggaatat tgattcctgt cacagctaat
    49621 ccgtagattc ctgcgcctaa aatgattccg cacaattctg taaataaatt taattttttc
    49681 atttctagcc tcttttcttt tgaaaatttt cagcaacttc tttattatac tgacttatac
    49741 tcttacttca agaggaaaat gaaaaaaaat tatttttggc tgtttctttt tatttatcaa
    49801 ataaggagtg gaaaaatgcg agcagcagac ttattgacaa ttcttacaaa atcacccaaa
    49861 aaaacagagt atcaaggagt ttttttggct caagaacaaa aactggtgcc tgtgacaatg
    49921 gtccagataa ataaagcaaa tcaatttatc tttttatttg aacacggcaa agcaccactt
    49981 gctatgaaag acatctttct ttttttgatg aagaaccgtg agaaagctgt ttattatcag
    50041 tcaaaagaag aactcattcc tctttatgga gtgaaagaga tagaaaataa agtggtgata
    50101 taagtaaaaa taagaaaaca agacgtttat gatgtcaaaa caaccatttt ttacattttt
    50161 ggcaaaaaag tgtcatgata cagtataata ttcgttgaac aggccaataa aggtgttctc
    50221 ccccacgaga tttctacttt attggctgtt tctttttatt aaaataaaag ttgtgtgtgt
    50281 aaaaaagata tagctgattc caagtgacaa tttcgtatag atttttatgg tattcgtagt
    50341 gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct tggattcttt ttttgtcagc
    50401 atatttagag gtattttttt cttttttttg ttcgtgctgt aaggttaagg caagacaagt
    50461 tagttgggaa aaaggaaaga aatgtttata cttaaagaat aacagaaagg aaggtcgcat
    50521 gaataatttt atacaaacat taagtgaaag aaaaggcgag ttgctggaag ctgcatttca
    50581 acatctgtcc atttctctca tttctttgct gattgcagca ctgattgcta ttcctttagc
    50641 gatttgggca gccaatcata aaaaaatggc agaagtctta ttgcagatca cgggtgtgct
    50701 ccaaacgatt ccttcacttg ccttattagg tttacttatc ccatttgtag ggatcggaac
    50761 tgttccagca ttgattgcac ttgttattta cgctttgctt ccgattttcc aaaacacgta
    50821 tattggtcta gctgaaattg acccgtctat tgaagaagca gcggtagcat ttggtatgtc
    50881 cagaatgaga cgcttgctga aagtagagct tcccatcgca ttacctgtca ttatctcagg
    50941 tatccgaact gccttagttt taatcatcgg tacggctact ttggcagcat tgatcggagc
    51001 aggtggatta gggacattta ttttgttagg tattgaccga aatacacctg ttttgacatt
    51061 gattggtgcg atcagttctg ccttgcttgc aattatattc agcggactga ttcgctggtt
    51121 gcagcataag aaacctcgat attccatcat tactttagca gcagtggtgt gtttatttgg
    51181 cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa accatcacga ttgctggaaa
    51241 attaggagcg gaacctgaaa tcctgatcca aatgtataaa gaattgattg aagacaacac
    51301 aaaaacagaa gttgaattaa aaccgaattt tggcaagacg agttttttgt tcaatgcact
    51361 ggatcaaggt caaatcgata tctatcctga gttcacaggt actgtcctag aaagcttggt
    51421 gaaggtacct gaagcaatga aaagtgctga tttgacaaaa gaaaaaacat atgacgaagc
    51481 agaagaatta ttgaataagc aattcgatat gacattatta aaaccaatgg cttaccaaaa
    51541 tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa catcaactta aaaagatttc
    51601 cgatttggca gctatctctg atcagatcaa agcgggattc acactggaat ttattgatcg
    51661 ttcagatgga tataaaggaa tccaagaagc atatggcttg aatttttcaa gtgtacaaag
    51721 catggagcca agtttgcgtt accaagcaat caataatgga gatgtcaata ttgtggatgc
    51781 ctattccaca gatagccagc tcaaggagta cgatctagtg actctagaag atgatcgagg
    51841 gttattccca gcttatcaag gcgcaccgct gatgaaaaaa gattttgcta aagaacatcc
    51901 tgaaatcgtt aaagcactga ataaattaag cggaaaaata acagaggatc aaatgattga
    51961 tatgaattac caagtcaatg tcgaaaagaa aaagccgaca gaagtagcac gaatcttcct
    52021 gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta aattaatcga gttcaaacat
    52081 gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc taaatctggc aatcaaaaaa
    52141 ggagaaatct ttgttttggt tggtccttcc ggtagtggaa aaacaactac tttgaaaatg
    52201 atcaatggat tatctaaacc atcagcagga gacatctact tcaaaggaaa atcgttaaat
    52261 gaatataatc tgcagaaaat gcggtggaat atgggatatg tgctgcagca aatcgctttg
    52321 ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc ctgaaatgtt aggttgggaa
    52381 aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa aagttggctt atctcctgat
    52441 atttatcgag atcgtatgcc tagagagttg tctggaggag aacagcaacg gatcgggatc
    52501 atccgagcta ttgctgcaag cccagatgtg attttgatgg acgagccatt tagcgcatta
    52561 gatccgattt caagaaactc attacaagaa ttggttttgt ccttacacga agaattgggc
    52621 acgaccatcg ttttcgttac gcacaatatg gaagaagcaa tcaagctagg tgatcgtatt
    52681 gcatttatga aggatggaga aattatccaa tgtgacacac cagagcagtt attgatgaat
    52741 cctaaaaacg actacgttcg gcatttcttt gatgaaccga aacagacgaa agagtggcga
    52801 gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa taccagaaaa tgctcgtcaa
    52861 caagtttgct ttgatacgcc tatgaaagaa gtatattcgc ttctttcggt gtatccaagc
    52921 attgtgatag tagaaaagca aagaagtatt gggtcgctga caagtaaaga aatctttgct
    52981 tttttaagtc gagaggagga aggaaatgaa aacatatgat gcaatcatca tcggttcagg
    53041 tgtgagcggc ttaagtgctg cttatggatt gaaagaagca ggtaaaacag tactagtggt
    53101 cgaagaagat ctatggggcg ggacttgtcc taaccgtgga tgtgatccta aaaaagtctt
    53161 gttaagtgca gtagaggcac gtaatcgggt aaaacaattg agtggaaaag gttttaacga
    53221 gatcccaact gccaactggg aagaacttca aaaattcaaa cgaacattta cggatccagt
    53281 cccagaaagc cgaaaaaaac aattagcaga agctgaaatc gatcatcttt caggaaccgc
    53341 tcgttttttg gatgattcat ctattgaagt caacgaagaa gtcttccatg cggattactt
    53401 agttttggca accgggcaaa gacctacgat cttaccagtg gagggaaagg aatacttgaa
    53461 aactagtgca gactttttat cattgcctgt actgcctaaa gaaatcattt ttatcggtgg
    53521 tggttatatt gcttttgagc tagctacgat tgccaatgct gcagggagca aagtaaccat
    53581 tgtccatcat aatcaacggc cattaaaaga atttgaggcg tctttggtag aggaagccgt
    53641 ccatcaaatg gaagcatctg gcattcagtt tgcttttggt gttgaaaccc aaaagatcat
    53701 ttcagaaggt actcgctatc gtttagtcgg aaaagagacg gaattagtag cagatatgat
    53761 tttttgtgcg actgggagac agccgaatac agaatcttta gcacttgagc aagcgaatat
    53821 cgtctttgat aaacacggaa ttgcagtcaa tgattatctg caaacgagca atccaaaaat
    53881 ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa ttgacaccag tcgccacttt
    53941 tgaaggaaat tatgtagcta agcggataac ggatgcaaca agcgaaccta tcaaatatcc
    54001 gattatacct acgatcgtct acgccagtcc taaacttgct gaagtaggag ttacaaaaag
    54061 tcacgcctct tcgtcagatc aagtggtaga gatggatctt accagttggt tcacctatca
    54121 tcgtgtcaat gaaccagttg ctaaggctga attgaccttt gaccagcaaa attatttgat
    54181 tggtgctgca gtgattagcg agcaagcaga cgaattgatc gatgatttga cactcgtcat
    54241 caatcaaaaa ttgactaaaa aagagttgga tagctatatc atggggtatc cgacattggc
    54301 aagtgatctc tcctatttat tgaaataatc agcatgataa ctaaagcgct tgttgctttt
    54361 tccttcttgt gctacacttt aaaaaatgaa tacctgtttc tggggatgtc gtagacgaca
    54421 tgagagtaag ttgtgaactt agacccattg aacctgtcag ttaatactgg tgaagggaga
    54481 aacatagcag acgcttgaag cgggttacta tgtcgtgccc atttcaagcg ttttttgtta
    54541 ttcatattag aagaaaggag gtaacaaaat gaagaaaaat ctttatccag cagtcagtat
    54601 ggcattcatt ttaattattt ggcagattgt tgtcatagtt tggaaaatac ctgcctttat
    54661 tttacccgga cctgctgctg tattttctgc attagcggca gatgcacaag tattactttc
    54721 ccattctgtc acgacattaa atgaagcagt gcttggtttg gtaatagcta gcgttctttc
    54781 ttttattact gccttggcaa tggattattg gaagttttta gaaatgtctt tttatccgtt
    54841 actagtgatt tcacaaatgt tgccaatcat ggtgttaggg ccattactta ccctatggtt
    54901 tggtttcggt atgcttccaa aagtgatcct tgttgtcttg ataagctatt ttccgatcgt
    54961 agtttctttt tcggatacat tgaggaaagt atcaaaagag caaatcatct ttttgaaaac
    55021 gatgggggca gatacaggga agatttatcg aatatataaa ataccagcgg gtttatccgg
    55081 cttcttttcc ggtttaaaag tggctgctac ttattgtgtc agcggagcag tcgttggaga
    55141 atggctaagc gctcaagcag gcttagggta ttacatgata cgcgtaaaaa acagctacca
    55201 gatcgataaa gtttttgctg cgatcatttg tgtcattcta ttgagtttat tgctgaatgg
    55261 cgcttgttca ttattgaaaa aagggtatta tcgtatgctt tatatttttt aagagaaggg
    55321 gatcaaatga aaaaattatg gcttttatta ccgctactgt tgcttttatc cgcttgtggc
    55381 actgcaaaag aaacaagtag caaacaagaa ataaaggatt tgaaaaaagt gacacttgtt
    55441 ttggattatg taccgaatac taatcatact ggcatctacc tagcaaaaga aaaaggctat
    55501 tataaagagg ctggtttaaa tgtccaaatc atcgaaccag gagataattc gacaagtatt
    55561 ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt atcaagaaga tgtcacttat
    55621 gctcacgcag atggacagaa tataccagtc aaagcaattg ctacagtgat caaacataat
    55681 acttctggat tcgccaccct atcagacagt aacatccatt caccaaaaga ttttgaagga
    55741 aaaacatatg caggttggca aagtccaagt gaagaagctg tcttaaaagc tgtgatggaa
    55801 aaagacggcg gggacttttc aaaactgaca atggtaggaa gcaatggaga agggccagaa
    55861 agcttaggaa aatcctcaga tatccaatgg tactttgaag gttgggatat gatcaaagca
    55921 aaagaagcgg gtattgaact aaactatatc ccattgaaag aattagatga acggttagat
    55981 tactatactc cggtaattat tacgaatgat caactgatca aaagtgatcc agaattggta
    56041 cagtctttta tggatgccac gaaaaaaggc tatcaggaag ctattaagga tccaaatgac
    56101 agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc ggacgttttt agaagaatca
    56161 caagcattct tgtcaaaaaa ttatacggat gatcctgaga attggggatt gatggaagaa
    56221 aaagtttggc gcaattatac agcttttatg caggaaaacg gattgatcaa gcaagatgta
    56281 ccttcgcaag aactattcac taatcagttt ataaaatagg agtgatagat atgtcaatcc
    56341 aaatcagcca cttgaatgta gtcattgaaa acctgaaaat attagaagac atccatttca
    56401 ctatccctaa agataccttt acatcgattg tcgcgccaag cggagccggt aagtcaacat
    56461 tgctgaaaac tttaacagga gtccagccta tgacttctgg aacgatcaaa gtagacgatc
    56521 aaaaagtaac tggtctccat acagcattta gttatatgcc gcaagaagat atgttacttc
    56581 cttggctgag cgtctatcaa aacgttacgt tatatcaaaa aatcaatcat ctaacaatca
    56641 atgaagaaca agtaagggat tatctggata tctttgggtt aaacggctat gaacattttt
    56701 tgccggaaca attatctggc gggatgaagc agcgaacggc cttgttacga acaatcatga
    56761 atccttcttc ttacttgctt cttgatgaac catttggagc gttagatgct atgaccagag
    56821 ggcagatgca agactggatg ttgaagctgc ctaaaaaagc aaagcggact actttacttg
    56881 tgacgcatga tatcgaagaa gcaatttatt tatcagacag gattcttgtg ctttccgccc
    56941 gtccggcaca tgtgattgct gagattcaag tgcctgaaaa acgacggtca cgtgaatggc
    57001 tactgcagca atcagagttg aagcaaacga tttatcagtt attggcaggt gaattagatg
    57061 ttaagtgatg ctcattgtca tttagatggg agtcagagtt tagcactcct tcagcaggaa
    57121 cacacgattt tgacgatcat caattgtgat tcgccagaag aatggaagga aaacagacag
    57181 cttgcagcaa gtaagactca agccttaagt tacggtatcc atccttggaa agcagatagc
    57241 tatacgtttg aacaagttga gccttttttg aaaaaagcaa ggatcatcgg tgaaataggg
    57301 ttagataata tttggacgaa tgtaccgatg actacacaaa aaaaagtatt tgagcgacag
    57361 cttgcttttg cagccataaa tgagaaacct gttgtactgc acacaaaagg atgcgaaaaa
    57421 gagatcttaa attatatcca agcttaccct aatcactatt tgatccattg gtattcttct
    57481 ttggactatc aacaagacta tattgattta ggctgttatt tcagcattgg tcttgatttg
    57541 aaaaagaatc cagcagtctg gcaactagct aaagcagtcc caatcgacca cttacttatt
    57601 gaaacggatg gactacatgc agcagagtgg gctttagggg ggattttttc agaaaaagat
    57661 tatgtctcac tgcttgaaga acagttaaag acaatcgctg agatcaaaga gctatcagtt
    57721 gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt tcattagatt aggtaaataa
    57781 aaaagagatg tgacacaact agtgtcgcac ctctttttgg ctttctttgt ataaaagtaa
    57841 actgtttgat taataaatga atcttagtac caaccgtttg cttcccagaa tgctt
//
LOCUS       DACEUF010000219        20985 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.guided.046, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000219 DACEUF010000000
VERSION     DACEUF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.guided.046"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(134..577)
                     /locus_tag="IXG20_002778"
     CDS             complement(134..577)
                     /locus_tag="IXG20_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1610884.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IXG20_002779"
     CDS             complement(795..2135)
                     /locus_tag="IXG20_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1610885.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IXG20_002780"
     CDS             2404..3300
                     /locus_tag="IXG20_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1610886.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IXG20_002781"
     CDS             complement(3450..4391)
                     /locus_tag="IXG20_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1610887.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IXG20_002782"
     CDS             4517..5638
                     /locus_tag="IXG20_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1610888.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IXG20_002783"
     CDS             5896..7398
                     /locus_tag="IXG20_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1610889.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IXG20_002784"
     CDS             7465..7992
                     /locus_tag="IXG20_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1610890.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="IXG20_002785"
     CDS             8203..9264
                     /locus_tag="IXG20_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1610891.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="IXG20_002786"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="IXG20_002786"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1610892.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="IXG20_002787"
     CDS             10177..12159
                     /locus_tag="IXG20_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1610893.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IXG20_002788"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IXG20_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1610894.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="IXG20_002789"
     CDS             12881..14305
                     /locus_tag="IXG20_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1610895.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="IXG20_002790"
     CDS             complement(14486..15259)
                     /locus_tag="IXG20_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1610896.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="IXG20_002791"
     CDS             complement(15256..16161)
                     /locus_tag="IXG20_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1610897.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSYKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSIKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="IXG20_002792"
     CDS             complement(16315..16635)
                     /locus_tag="IXG20_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1610898.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17109..17795
                     /locus_tag="IXG20_002793"
     CDS             17109..17795
                     /locus_tag="IXG20_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1610899.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17797..18741
                     /locus_tag="IXG20_002794"
     CDS             17797..18741
                     /locus_tag="IXG20_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1610900.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18940..19584
                     /gene="pcp"
                     /locus_tag="IXG20_002795"
     CDS             18940..19584
                     /gene="pcp"
                     /locus_tag="IXG20_002795"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1610901.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19663..20940
                     /locus_tag="IXG20_002796"
     CDS             19663..20940
                     /locus_tag="IXG20_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610902.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6779 a         3783 c         4361 g         6062 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaaaaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttatt gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgatgga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttataac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    17161 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    17221 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    17281 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    17341 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    17401 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    17461 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    17521 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    17581 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    17641 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    17701 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    17761 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    17821 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    17881 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    17941 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
    18001 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    18061 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
    18121 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
    18181 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
    18241 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
    18301 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
    18361 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
    18421 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
    18481 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
    18541 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
    18601 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
    18661 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
    18721 tatatgattc tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct
    18781 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
    18841 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
    18901 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
    18961 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
    19021 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
    19081 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
    19141 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
    19201 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
    19261 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
    19321 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
    19381 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgcca
    19441 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
    19501 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
    19561 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
    19621 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
    19681 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
    19741 caatacacaa gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa
    19801 ctagtcgata agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta
    19861 gctgcaacag attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga
    19921 agccagcaaa aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa
    19981 tcgccaaaaa ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat
    20041 gtctctccga cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta
    20101 gacgaattat tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa
    20161 tactgttttg tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa
    20221 ttagctcagc ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat
    20281 caagcattgg aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag
    20341 cgacagataa aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa
    20401 gaattcttga tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa
    20461 gaagtgccag tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca
    20521 aaacgtcaac gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga
    20581 ttcatccaac caaaaatcta tctatttatt tggaagcaat attgtcgcta tggttttaca
    20641 gaagggttct tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt
    20701 aaagaacgag aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa
    20761 tggcaactag tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg
    20821 aaaggaaaac attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag
    20881 ttagaagaat cactgcgttt caatgatgag gcaagaaagt atctgaagga attactataa
    20941 aaaatagagg ctgggacaga agcgtttaac tccaagaaat aagaa
//
LOCUS       DACEUF010000220        20863 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.guided.047, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000220 DACEUF010000000
VERSION     DACEUF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.guided.047"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            267..1304
                     /locus_tag="IXG20_002797"
     CDS             267..1304
                     /locus_tag="IXG20_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1610903.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="IXG20_002798"
     CDS             complement(1393..1716)
                     /locus_tag="IXG20_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1610904.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="IXG20_002799"
     CDS             complement(1703..2410)
                     /locus_tag="IXG20_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1610905.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="IXG20_002800"
     CDS             2496..2837
                     /locus_tag="IXG20_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ1610906.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="IXG20_002801"
     CDS             complement(3008..3856)
                     /locus_tag="IXG20_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610907.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="IXG20_002802"
     CDS             4454..5317
                     /locus_tag="IXG20_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1610908.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="IXG20_002803"
     CDS             5417..5899
                     /locus_tag="IXG20_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1610909.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="IXG20_002804"
     CDS             complement(6162..6629)
                     /locus_tag="IXG20_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1610910.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="IXG20_002805"
     CDS             6752..7603
                     /locus_tag="IXG20_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1610911.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="IXG20_002806"
     CDS             complement(7854..9812)
                     /locus_tag="IXG20_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1610912.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="IXG20_002807"
     CDS             complement(9809..10681)
                     /locus_tag="IXG20_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1610913.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="IXG20_002808"
     CDS             complement(11023..11895)
                     /locus_tag="IXG20_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1610914.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="IXG20_002809"
     CDS             12023..12592
                     /locus_tag="IXG20_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1610915.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="IXG20_002810"
     CDS             12659..13396
                     /locus_tag="IXG20_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1610916.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="IXG20_002811"
     CDS             13627..14895
                     /locus_tag="IXG20_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1610917.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="IXG20_002812"
     CDS             15382..15846
                     /locus_tag="IXG20_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1610918.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="IXG20_002813"
     CDS             15830..16459
                     /locus_tag="IXG20_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1610919.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..18291
                     /gene="msr"
                     /locus_tag="IXG20_002814"
     CDS             16813..18291
                     /gene="msr"
                     /locus_tag="IXG20_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1610920.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            18338..18784
                     /locus_tag="IXG20_002815"
     CDS             18338..18784
                     /locus_tag="IXG20_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1610921.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18983..19498)
                     /locus_tag="IXG20_002816"
     CDS             complement(18983..19498)
                     /locus_tag="IXG20_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1610922.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            19615..20817
                     /locus_tag="IXG20_002817"
     CDS             19615..20817
                     /locus_tag="IXG20_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1610923.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         6632 a         3703 c         4315 g         6213 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt gtggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg atgttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacag ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtata ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatgt tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
    17701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
    17761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
    17821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
    17881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
    17941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
    18001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
    18061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
    18121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
    18181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
    18241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
    18301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
    18361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
    18421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
    18481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
    18541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
    18601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
    18661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
    18721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
    18781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
    18841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
    18901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
    18961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
    19021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
    19081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
    19141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
    19201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
    19261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
    19321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
    19381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
    19441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
    19501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
    19561 tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
    19621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
    19681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
    19741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
    19801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
    19861 cggatcggtt tgaaaaaaac attgatgaca ggattgttta tcatcgttgt attcgctggt
    19921 cttggcggat tatctgggaa tatcggaatg ctgattgggt tacgtgccgg ctggggtgtc
    19981 gggaatgctt tatttgtctc tactgcttta gcaacgatcg tcagtatttt agttggtaac
    20041 acagaaaaag caatcatgat gtatgaagca gcacttggct gcggaatggc tgttggccct
    20101 ctagtcggtg gggtcttggg cagtggttca tggcgttatc cattttttgg ggtagcattt
    20161 ttgatgtttt gcggttttat agccctagcg gtacttttac ctgaaactga gaaaccaaag
    20221 agaaaagtag gcctttggga aggtgtcaaa gcgttaagca atcataaact tcgttcagtt
    20281 ggactaatcg cattgttata taatttcgga ttctttactt tacttgcgta tgcgccattt
    20341 ttattgattg gctattctga attagaagtc ggttttgtat tttttggctg gggtgtgctt
    20401 ctggctattg cttcgatttt cgttgcaccg gctcttgagc ggaagtttac gacttatcgg
    20461 acaattttag cagcatttgt cttattcatt atttgtttgc tgttattagg agctggaact
    20521 tctatgccag tccttgtacc gatcagcatt gtgttagcag gatttttcca aggaattatc
    20581 aatacactgt tgacgacgat tgctatggaa ataccaggtc tgcaacgaaa cgttgcttcg
    20641 tccagctata gttttgtccg ctttttcggt ggagctttag caccatttat tgcaggtgtg
    20701 attggtgaac atattggtga aagctatgcg ttttattttg caggtgccat tgttcttatc
    20761 agtgtatgtt gtatcattta tcaccgtaat tattttgcta caaatgaaga agaataagaa
    20821 atcagaggtt gtgaaaaagc tgtcctgcat caagtcataa gaa
//
LOCUS       DACEUF010000221        11428 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.guided.083, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000221 DACEUF010000000
VERSION     DACEUF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.guided.083"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            39..977
                     /locus_tag="IXG20_002818"
                     /pseudo
     CDS             39..977
                     /locus_tag="IXG20_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            980..3163
                     /locus_tag="IXG20_002819"
     CDS             980..3163
                     /locus_tag="IXG20_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1610924.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3160..4161
                     /locus_tag="IXG20_002820"
     CDS             3160..4161
                     /locus_tag="IXG20_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1610925.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4176..5090
                     /locus_tag="IXG20_002821"
     CDS             4176..5090
                     /locus_tag="IXG20_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1610926.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5335..5451
                     /locus_tag="IXG20_002822"
     CDS             5335..5451
                     /locus_tag="IXG20_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1610927.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5467..7386
                     /gene="tet(M)"
                     /locus_tag="IXG20_002823"
     CDS             5467..7386
                     /gene="tet(M)"
                     /locus_tag="IXG20_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1610928.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7484..8857
                     /gene="tet(L)"
                     /locus_tag="IXG20_002824"
     CDS             7484..8857
                     /gene="tet(L)"
                     /locus_tag="IXG20_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ1610929.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9421..10683
                     /locus_tag="IXG20_002825"
     CDS             9421..10683
                     /locus_tag="IXG20_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ1610930.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10928..11410
                     /locus_tag="IXG20_002826"
     CDS             10928..11410
                     /locus_tag="IXG20_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ1610931.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNGSVAKF
                     "
BASE COUNT         3772 a         1910 c         2388 g         3358 t
ORIGIN      
        1 acttggagat aacatcaaaa agttacctat cgctataacg tggcagatgg aaagaaacct
       61 tgccagagat ttcccatgaa atcaatatcg tcaatctgac ttctgatgag aaaaacaaag
      121 gcttacttga cccttatgta attatgaaaa ataccaaaga ttctgaatca ctggctattg
      181 atattttgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc ccaatcctta
      241 gaaaagccat tcgtgcagta accaatagtg aagtgcgagg gttgatgaaa gtgattgaag
      301 aattacgggt tgagaatacg ccactaagta ccagcatagc cgaccatatc gaaagtttta
      361 cagactatga ctttgcccat ctgcttttta gtgatggtta tgtagagcag tctatcagcc
      421 ttgaaaaaca actgaacatt atacaggttg ccgacttggt acttcctgac aaggaaactt
      481 cctttgagga atataccaca atggagttac tatcggtagc aatgctgatt gtcattagta
      541 cctttgcgtt ggactttatc catacagacc gaagtatttt caagattgta gacttagacg
      601 aagcatggag cttcttacag gtggcacaag ggaaaacact atctatgaag ctggttcgtg
      661 ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagat gacctcttag
      721 atgaaaaact gaaaaataac ctcggcttaa aatttgcctt tcgttccact gaccttaacg
      781 agattaaaaa aacccttgcc ttttttggtg tagacccaga ggacgaaaac aatcagaagc
      841 gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt cgtgtcggtg
      901 tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc agaccacctg
      961 tgcgaaaaga ggtgtaaatg tgaaaccatc aatattaaac agaataaaat caaactggac
     1021 gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga ttgccatttt
     1081 tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg atacggtcaa
     1141 tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt tttatgtgga
     1201 taatagctgg ggctggctac cgtggaactg gtcggacgga attggaaaac aagtcatgta
     1261 tggactatat gccattacca attttatttg gacaatcagt ctttatgttt ccaatgcgac
     1321 gggttactta gtacaggaag cctattcctt agacttcatt tccgctacag cagattccat
     1381 tggcaagaat atgcagaccc ttgcaggggt tacgcctagt ggactatcaa cagagggttt
     1441 ctatgttgga ttccttttac tcttgatttt ggttcttggg gtttatgttg cctatacagg
     1501 actgataaag agagaaacca caaaggcaat tcatgccctc atgaattttg tgctggtttt
     1561 tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa tcaatgactt
     1621 ttcatcagac atcagcaacg ctagtttgtc actgggtaca aaaattgtca tgccccattc
     1681 agatagtcaa ggcaaggaca gtgtggactt gatacgtgac agcctatttt ccatacaggt
     1741 tcagcaaccg tggctactgc ttcaatataa cagttcagac attgaaagta ttggtattga
     1801 ccgtgtggaa agcctgctct caaccagtcc agattccaac aatggggaag acagagaaaa
     1861 aattgttgcg gaagaaattg aagatagaag caataccaat atgacgatta caaagacgat
     1921 taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt ccatatttgt
     1981 attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct atgctatgtt
     2041 tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt caaaacgagc
     2101 cataacaaag ctctttaata ccattttgac acgagctgga atcacattga ttattacgac
     2161 agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt tctttttgat
     2221 tgcttttcta cagattgtga cctttgcagg aatctacttt aagctgggcg atttaatgag
     2281 tatgttttct ctacagagta acgattctca aagtgtggga agtcgtatga tgagaaaacc
     2341 tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac gctccgtgac
     2401 tgctttaggg gctgggtctg ccattgccag tgctacgggt aaaaaaggac agtcgggttc
     2461 gggaagttct gcgagtacac aagcagatca ctctcgacca aacgggcagg aaaaatcaac
     2521 actaggaaag cgtatcggtc aaaccatcgg tacagtagct gataccaaag acagaatggt
     2581 agatactgct ggtaatttga aagaacaggt taaggatttg ccgaccaatg caagatatgc
     2641 agtatatcaa ggaaaatcta aagtgacgga taatgtccat gatttaacca gaagtatttc
     2701 tcaaaccaaa gcggataaag ccagtggacg caagaaacag caggaacaaa gacggaaaac
     2761 cattgcggag cgtcgctctg aaatggaaca ggtcaaacag aaaaaacagc cagcttcttc
     2821 cgttcatgaa agaccagcca ctaagcaaga acaatctcat gatggacaga cttcaaggca
     2881 aacaactgta caggcttcct atagggaatc tcaacaagcc aaacaagagc gtccaacagt
     2941 taagtccgat tcttcaagtt taaaaacgga acgtcaaagt aatacagttc aagaaagaac
     3001 ggttcaaaag ccagtaactt caactgcacc aacagataga gcttcacaac gttcaatcac
     3061 aaaagaacgt cagtctactg ttcaaagagt accactacaa aatacaaaaa gtagaccacc
     3121 aatcaaaacc gccaccatta agaaaggcag taagaaacca tgaagctgaa aactttagtg
     3181 attggtggtt cgggattatt cttgatggtc ttctcactgc ttctgtttgt cgccatttta
     3241 ttttcagatg aacaagatgg cggtttttcc aacattcact atggcggtgt ggatgtttcc
     3301 gcagaagtgc tggctcataa gcctatggta gaaaaagttg ccaaagaata tggaattgaa
     3361 gaatacgtca atatacttct tgcgattata caggtggaat cgggtggtac agcagaagat
     3421 gttatgcagt cctcggaatc cctcggtctt ccacctaatt cattgagtac agaagaatcc
     3481 attaagcaag gtgtgaagta tttcagtgag ttattagcca gtagcgaaag gcttggtgta
     3541 gatttagaat cggttatcca gtcctacaat tatggtggtg gtttcttagg atatgtggct
     3601 aatcgtggga ataaatatac ctttgaactg gctcaaagtt tctcaaaaga gtattcgggt
     3661 gacgaaaaag tgtcttaccc caatcccata gccataccta tcaatggggg ctggcgatac
     3721 aattacggga atatgtttta tgtgcaactg gtaacgcagt atcttgtcac aacagagttt
     3781 gatgatgata cagtacaagc catcatggac gaagctctga aatttgaagg ctggaagtat
     3841 gtctatggcg gagcttcccc gactacctct tttgattgta gcggactgac acaatggacg
     3901 tatggaaaag ctggaatcaa cttaccacga acagcacaac agcaatatga tgtaacccgg
     3961 catatcccat tattggaagc aaaagccggc gatttggttt tctttcattc tacctataac
     4021 gctggctctt atattactca tatcgggatt tatttaggcg ataaccgtat gtttcatgca
     4081 ggcgacccta tcggttatgc cgacttaaca agctcttact ggcaacagca tttagtggga
     4141 gcaggacgaa tcaaacaata agaaaggacg attcaatgat gaaattcgga aaaaatcaga
     4201 atacagaaaa acagacacca aaggaaaaga aacctcgtgt ctataaggtc aatcctcgta
     4261 aaaaggttgt gattgtccta tgggtacttt taggacttag tttcagcttt gcgatattca
     4321 agcactttac agccattgat acccatacca ttcatgaaac caccatcata gaaaaggaat
     4381 atgtcgatac tcatcatgtg gaaaattttg tagagaactt tgcgaaagtc tactattcat
     4441 gggaactaaa cgacaagtcc attgataatc gcatagaaaa cctgaaagca tatctgacag
     4501 aggaacttca agctctgaat gtcgatacag ttcgtaaaga tattcctgta tcgtcttctg
     4561 taaaaggatt tcagatatgg acggtagaag cagatggcga caatcaattt gatgtaacct
     4621 acagtgtaga ccaactcatt acagaggggg aaaatacaaa gaccgtccac tctgcttata
     4681 tagtgagtgt ctatgtagac agtactggaa atatggtcct cattaagaat ccgaccatta
     4741 ctagcatacc aaagaaatca gactataaac caaaagctat tgaaagtgag ggtacggttg
     4801 attccattac aaccaatgaa atcaatgagt ttttaacgac gttcttcaag ctctatccta
     4861 cagcgacagc cagtgaactt tcctactatg tgaatgacgg gatattaaaa ccaatcggaa
     4921 aagagtacat ctttcaagaa ctggtaaatc ctatttacaa tcgtaaggat aatcaagtca
     4981 cggtatcgct gacagtggag tatatcgacc agcagaccaa agcaacgcag gtatctcaat
     5041 ttgatttggt acttgaaaag aacgggagta attggaagat tatagaataa caaatattgg
     5101 tacattatta cagctatttt gtaatcacgt actctctttg ataaaaaatt ggagattcct
     5161 ttacaaatat gctcttacgt gctattattt aagtatctat ttaaaaggag ttaataaata
     5221 tgcggcaagg tattcttaaa taaactgtca atttgatagt gggaacaaat aattggatgt
     5281 ccttttttag gagggcttag ttttttgcac ccagtttaag aataccttta tcatgtgatt
     5341 ctaaagtatc cggagaatat ctgtatgctt tgtatgccta tggttatgca taaaaatccc
     5401 agtgataaga gtatttatca ctgggatttt tatgcccttt tgggcttttg aatggaggaa
     5461 aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcggg aaaaactacc
     5521 ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag cgtggacaaa
     5581 ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac aattcagaca
     5641 ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac gccaggacat
     5701 atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc aattctactg
     5761 atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc acttaggaaa
     5821 atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat tgatttatca
     5881 acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa acagaaggta
     5941 gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg ggatacggta
     6001 atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt agaagcattg
     6061 gaactcgaac aagaggaaag cataagattt cagaattgct ccttgtaccc tgtttatcat
     6121 ggaagcgcaa aaagcaacat agggattgag cagcttatag aagtgataac gaataaattt
     6181 tattcatcaa catacagaaa gaagtctgaa ctttgcggaa atgtcttcaa aattgaatat
     6241 tcggaagaaa gacaacgtct tgcatatgta cgcctttatg gcggaatcct gcatttgcgg
     6301 gattcggtta gaatatcgga aaaggaaaaa ataaaaatta cagaaatgta tacttcaata
     6361 aatggtgaat tatgtaaaat tgataaggct tattccgggg aaattgttat tttgcaaaat
     6421 gagtttttga agctaaatag tgttcttgga gatacaaagc tattgccaca gagagagaga
     6481 attgaaaatc cgctccctct gctgcaaaca actgttgaac cgagcaaacc tcaacaaagg
     6541 gaaatgttac ttgatgcact tttagaaatc tccgacagtg acccgcttct acaatattat
     6601 gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg
     6661 attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc
     6721 atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca
     6781 aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg
     6841 cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg
     6901 gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa
     6961 atctgtttta agtatggctt atactatagc cctgttagta ccccagcaga ttttcggatg
     7021 cttgctccta ttgtattgga acaagtctta aaaaaagctg gaacagaatt gttagagcca
     7081 tatcttagtt ttaaaattta tgcgccacag gaatatcttt cacgagcata caacgatgct
     7141 cctaaatatt gtgcgaacat cgtagacact caattgaaaa ataatgaggt cattcttagt
     7201 ggagaaatcc ctgctcggtg tattcaagaa tatcgtagtg atttaacttt ctttacaaat
     7261 ggacgtagtg tttgtttaac agagttaaaa gggtaccatg ttactaccgg tgaacctgtt
     7321 tgccagcccc gtcgtccaaa tagtcggata gataaagtac gatatatgtt caataaaata
     7381 acttagtgta ttttatgttg ttatataaat atggtttctt gttaaataag atgaaatatt
     7441 ttttaataaa gatttgaatt aaagtgtaaa ggaggaatta attatgaagt gtaatgaatg
     7501 taacagggtt aaatacaacc aaattttaat ttggctttgc gttttatctt tttttagcgt
     7561 attaaatgaa atggttttga acgtctcatt acctgatatt gcaaatgatt ttaataaacc
     7621 acctgcgagt acaaactggg tgaacacagc ctttatgtta accttttcca ttggaacagc
     7681 tgtatatgga aagctatctg atcaattagg catcaaaagg ttactcctat ttggaattat
     7741 aataaattgt ttcgggtcgg taattgggtt tgttggccat tctttctttt ccttacttat
     7801 tatggctcgt tttattcaag gggctggtgc agctgcattt ccagcactcg taatggttgt
     7861 agttgcgcgc tatattccaa aggaaaatag gggtaaagca tttggtctta ttggatcgat
     7921 agtagccatg ggagaaggag tcggtccagc gattggtgga atgatagccc attatattca
     7981 ttggtcctat cttctactca ttcctatgat aacaattatc actgttccgt ttcttatgaa
     8041 attattaaag aaagaagtaa ggataaaagg tcattttgat atcaaaggaa ttatactaat
     8101 gtctgtaggc attgtatttt ttatgttgtt tacaacatca tatagcattt cttttcttat
     8161 cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc aggaaagtaa cagatccttt
     8221 tgttgatccc ggattaggga aaaatatacc ttttatgatt ggagttcttt gtgggggaat
     8281 tatatttgga acagtagcag ggtttgtctc tatggttcct tatatgatga aagatgttca
     8341 ccagctaagt actgccgaaa tcggaagtgt aattattttc cctggaacaa tgagtgtcat
     8401 tattttcggc tacattggtg ggatacttgt tgatagaaga ggtcctttat acgtgttaaa
     8461 catcggagtt acatttcttt ctgttagctt tttaactgct tcctttcttt tagaaacaac
     8521 atcatggttc atgacaatta taatcgtatt tgttttaggt gggctttcgt tcaccaaaac
     8581 agttatatca acaattgttt caagtagctt gaaacagcag gaagctggtg ctggaatgag
     8641 tttgcttaac tttaccagct ttttatcaga gggaacaggt attgcaattg taggtggttt
     8701 attatccata cccttacttg atcaaaggtt gttacctatg gaagttgatc agtcaactta
     8761 tctgtatagt aatttgttat tacttttttc aggaatcatt gtcattagtt ggctggttac
     8821 cttgaatgta tataaacatt ctcaaaggga tttctaaatc gttaagggat caactttggg
     8881 agagagttca aaattgatcc tttttttata acaggaattc aaatcttttt gttccattaa
     8941 agggcgcgat tgctgaataa aagatacgag agacctctct tgtatctttt ttattttgag
     9001 tggttttgtc cgttacacta gaaaaccgaa agacaataaa aattttattc ttgctgagtc
     9061 tggctttcgg taagctagac aaaacggaca aaataaaaat tggcaagggt ttaaaggtgg
     9121 agattttttg agtgatcttc tcaaaaaata ctacctgtcc cttgctgatt tttaaacgag
     9181 cacgagagca aaacccccct ttgctgaggt ggcagagggc aggttttttt gtttcttttt
     9241 tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt tggtcggcac tgccgacagc
     9301 ctcgcagagc acacacttta tgaatataaa gtatagtgtg ttatacttta cttggaagtg
     9361 gttgccggaa agagcgaaaa tgcctcacat ttgtgccacc taaaaaggag cgatttacat
     9421 atgagttatg cagtttgtag aatgcaaaaa gtgaaatcag ctggactaaa aggcatgcaa
     9481 tttcataatc aaagagagcg aaaaagtaga acgaatgatg atattgacca tgagcgaaca
     9541 cgtgaaaatt atgatttgaa aaatgataaa aatattgatt acaacgaacg tgtcaaagaa
     9601 attattgaat cacaaaaaac aggtacaaga aaaacgagga aagatgctgt tcttgtaaat
     9661 gagttgctag taacatctga ccgagatttt tttgagcaac tggatccagg agaacaaaaa
     9721 cgattttttg aggaaagtta taaattattt tccgaacgat atggcaagca aaatattgct
     9781 tatgcaacag ttcataatga tgagcaaacc cctcacatgc atttaggtgt tgtgcctatg
     9841 cgtgatggaa aactgcaagg aaaaaatgtg tttaatcgtc aagaactgtt atggctacaa
     9901 gataaattcc ccgagcatat gaaaaaacag ggttttgagt tgaagcgtgg tgaacgtggc
     9961 tctgaccgta aacatattga gacagctaaa tttaaaaaac aaactttgga aaaagagatt
    10021 gattttctag aaaaaaattt agcagttaaa aaagatgaat ggactgctta tagcgataaa
    10081 gttaaatcag atttagaagt accagcgaaa cgacacatga aaagtgttga agtgccaacg
    10141 ggtgaaaagt ccatgtttgg tttgggaaaa gaaataatga aaacagaaaa gaaaccaacc
    10201 aaaaatgttg ttatatcgga gcgtgattat aaaaacttag tgactgctgc gagagataac
    10261 gataggttaa aacagcatgt tagaaatctc atgagtactg atatggcgag agaatataaa
    10321 aaattaagta aagaacatgg gcaagttaaa gaaaaatata gtggtcttgt agagcgattt
    10381 aatgaaaatg taaatgatta taatgagttg cttgaagaaa acaagtcttt aaagtctaaa
    10441 ataagcgatt taaagcgtga tgtgagttta atctatgaaa gcactaagga attccttaag
    10501 gaacgtacag acggcttaaa agcctttaaa aacgttttta aggggtttgt agacaaggta
    10561 aaggataaaa cagcacaatt ccaagaaaaa cacgatttag aacctaaaaa gaacgaattt
    10621 gaactaactc ataaccgaga ggtaaaaaaa gaacgaagtc gagatcaggg aatgagttta
    10681 taaaataaaa aaagcacctg aaaaggtgtc tttttttgat ggttttgaac ttgttctttc
    10741 ttatcttgat atatatagaa ataacgtcat ttttatttta gttgctgaaa ggtgcgttga
    10801 agtgttggta tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt gcacagaaaa
    10861 accccatctg ttaaagttat aagtgaccaa acaaataact aaatagatgg gggtttcttt
    10921 taatattatg tgtcctaata gtagcattta ttcagatgaa aaatcaaggg ttttagtgga
    10981 caagacaaaa agtggaaaag tgagaccatg gagagaaaag aaaatcgcta atgttgatta
    11041 ctttgaactt ctgcatattc ttgaatttaa aaaggctgaa agagtaaaag attgtgctga
    11101 aatattagag tataaacaaa atcgtgaaac aggcgaaaga aagttgtatc gagtgtggtt
    11161 ttgtaaatcc aggctttgtc caatgtgcaa ctggaggaga gcaatgaaac atggcattca
    11221 gtcacaaaag gttgttgctg aagttattaa acaaaagcca acagttcgtt ggttgtttct
    11281 cacattaaca gttaaaaatg tttatgatgg cgaagaatta aataagagtt tgtcagatat
    11341 ggctcaagga tttcgccgaa tgatgcaata taaaaaaatt aataaaaatg gttctgttgc
    11401 aaagttttaa ataaagaata aaatccct
//
LOCUS       DACEUF010000222         5926 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.guided.115, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000222 DACEUF010000000
VERSION     DACEUF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.guided.115"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            90..350
                     /locus_tag="IXG20_002827"
                     /pseudo
     CDS             90..350
                     /locus_tag="IXG20_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            513..1178
                     /locus_tag="IXG20_002828"
                     /pseudo
     CDS             513..1178
                     /locus_tag="IXG20_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            1214..1459
                     /locus_tag="IXG20_002829"
     CDS             1214..1459
                     /locus_tag="IXG20_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1610932.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1562..2431
                     /locus_tag="IXG20_002830"
     CDS             1562..2431
                     /locus_tag="IXG20_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1610933.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2412..3146
                     /locus_tag="IXG20_002831"
     CDS             2412..3146
                     /locus_tag="IXG20_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1610934.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            3179..4087
                     /locus_tag="IXG20_002832"
     CDS             3179..4087
                     /locus_tag="IXG20_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ1610935.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4084..4626
                     /gene="sat4"
                     /locus_tag="IXG20_002833"
     CDS             4084..4626
                     /gene="sat4"
                     /locus_tag="IXG20_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1610936.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            4719..5513
                     /locus_tag="IXG20_002834"
                     /note="aph(3')-IIIa"
     CDS             4719..5513
                     /locus_tag="IXG20_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1610937.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(5621..5809)
                     /locus_tag="IXG20_002835"
                     /pseudo
     CDS             complement(5621..5809)
                     /locus_tag="IXG20_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5789..5914
                     /locus_tag="IXG20_002836"
                     /pseudo
     CDS             5789..5914
                     /locus_tag="IXG20_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         2048 a          882 c         1283 g         1713 t
ORIGIN      
        1 gattttgtac aacccatcag tccttattga caaagagccg gaatttgtat cgataagggg
       61 tacaaattcc cactaagcgc tcgggacccc ttgtaggaaa atgtcctaag tgtggcaaca
      121 atattgtatt aaaaaaatcg ttttatggtt gttcaaatta tcctgaatgt aagtttactt
      181 tagctgaaca ttttagaaag aaaaaactaa ccaaaacgaa tgtaaaagaa ttactggagg
      241 gaaaagaaac cctggtaaaa ggaatcaaaa acaaagagaa aaagccctac aatgccgttg
      301 taaaaattgg ggaaaaggga tatattgatt ttatctcttt ttcaaaataa acatggggct
      361 gacccaaaag tagctcctat atgaaaaata gaggtctttc catttagata gtagtggaaa
      421 gacctctatt tatcttttga aagtgaatac aaaaataaag cacactctgc tataatatag
      481 gtgattaatc catttgagag aggtgcttta tcatgtatca aaattatacc ataaaccaac
      541 tttgtttgcc tatagatctt gaaattaaat tagaagaaaa tgattttgct catgccattg
      601 tgcagtttgt tgactctatt cccgatgaag tctttcttcc ttattatcaa tctatgggta
      661 gaccccaata tcatcctcgt atgctacttt ccattattct ttgtgcctat attcaagggg
      721 tttattcggg ccgccaaata caaaatatgc tgatagatag tattcggatg cgctacttat
      781 ctcaagagca attccctaac tttcgtacta ttaatcgttt tcgcgttcat ccgattatga
      841 acgatattct agatcattca tttgttcaat ttcgtgagct actggttcaa agtggcttaa
      901 tcagtggcag tgctctatac attgatggta caaaaattga agcggatgcg aataaatact
      961 ctttcgtttg gaaaaaaagt attttaaagt ataaggatag ccttgatcaa aaagcccttg
     1021 aaaactacca acaaatggtg gaaacaaaaa tcttacctga gttaatagat gatttaaagg
     1081 atgaactttc tatagaagag attgaaagga ttcgtcaatc tttggaagaa aaggaaaatc
     1141 aattaattga agcgattgac caaaccgaaa cagtagaagg gggctgaccc attattaata
     1201 aggagtggac aacatgattg atgtaaatga gctattggaa gaagctatta gagaaactga
     1261 aaatctaaat gatggcgaag tttttcttgt taaagattta ttcaagggat atgtggggaa
     1321 taggataccc agaaaggatc gacttctgct tggaacgtta tttttaaacc atgttagcaa
     1381 aatgaatggt aatttaaagg ctattgaaaa gacttcatct aatcagcaga ggtataaaaa
     1441 gacaattgat aagttttaac tatcttttgt gaaattaaaa aaattataaa tatgttgagt
     1501 agaagcaaaa gatttggctt ggaattttta cagcagtgaa tcgataaaag gagcatgctt
     1561 gatggtagat aatattatta aatcagtagc agagaaatta tcctctctgt cttatataga
     1621 aggtattgtt ttaggtggtt cacgtgcaag gggcacccat acagaggatt cggatataga
     1681 tatcggcatc tattacaatt cagaatcatt tgacataaat actattaatc aattcgctac
     1741 aaagctggat gatgagcata gaaataacct tgttgtacct cccggagcat ggggtgattg
     1801 gattaatggc ggcggatggt tagtcataaa cgggtatcat gtggatttaa ttttacgtga
     1861 tattaaacgt gtggaacaaa taatgaaaga tacagagcac ggaattgtta ctgccaatta
     1921 tcagactggg catccccatg gttatattag tgcaatgtat cgaggagaat tagcgattag
     1981 caaaatacta tatgctaaga atgaaagctt atgcgaatta aaaaaacagg cagaaactta
     2041 tcccaatgct ttgcagaaaa gtttagttaa cttttttatg tttgaagcag ggttctcttt
     2101 aatgtttgta aaagcaaatt cgggaacaga cgataaatat tatattgcgg gtcatgtttt
     2161 tcgtatagtt tcatgtttaa atcaagtgtt atttgcatgt aataatgctt attgtatcaa
     2221 cgaaaagaaa gctataaaac tgcttgaaac ttttgaacat aaacctgaaa aatataccga
     2281 gaaggtaaat catatttttg aagtactcgg tatctcactt tttgaatgct acgacatgac
     2341 cgagaagctt tataatgaag tgaatgaaat tgtatcggag ataaataact ttttaaacga
     2401 ggagagttca gatgaaagaa aacaaatatg atgataatat attttttcaa aaatacagtc
     2461 aaatgagtcg ctcgcagaaa ggactggctg gtgcgggaga atgggagact ttgaaaaaga
     2521 tgctacctga ttttaagggt aagcgtgtgc ttgatttagg atgcggctat ggatggcact
     2581 gtatatatgc gatggaaaac ggtgcttcct ctgtagtagg tgttgatatt tctcataaaa
     2641 tgctcgaagt agcaaaagga aaaacccatt ttccacagat tgaatatgaa tgctgtgcca
     2701 tagaagatgt ggatttccca gaggagagct ttgatgtaat actaagttcg cttgcgtttc
     2761 attatgtagc agactatgag aatttaataa aaaagatata taggatgctg aaggctggtg
     2821 gcaatttagt ttttacagtt gaacatcctg tttttactgc tcatggaaca caagactggt
     2881 attataacga aaaaggagaa atactgcatt tcccggtgga caattattat tatgagggca
     2941 aacggacagc tatgtttttg gaagaaaagg ttacaaaata tcatagaaca ctgaccacat
     3001 atctaaatac actgctttca aatagtttta taataaatca gattgtggag ccacagccgc
     3061 cagagaacat gatggatatt ccggggatgg cggatgaaat gcgacgccca atgatgctga
     3121 ttgtatcggc aaaaaagaag atgtaataat atagaaaaaa taaacgagga gtatgtaaat
     3181 gagatcagaa aaagaaatga tggatttagt actttcttta gcagaacagg atgaacgtat
     3241 tcgaattgtg acccttgagg ggtcacgcgc aaatattaat atacctaaag atgaatttca
     3301 ggattatgat attacatatt ttgtaagtga tatagaaccg tttatatcta atgatgactg
     3361 gcttaatcaa tttgggaata taataatgat gcaaaagccg gaggatatgg aattattccc
     3421 acctgaagaa aagggatttt cctatcttat gctatttgat gattacaata aaattgatct
     3481 taccttattg cccttggaag agttagataa ttacctaaag ggcgataaat taataaaggt
     3541 tctaattgat aaagattgta gaattaaaag ggacatagtt ccgactgata tagattatca
     3601 tgtaagaaag ccaagcgcaa gggagtatga tgattgctgc aatgaatttt ggaatgtaac
     3661 accttatgtt attaaaggat tgtgccgtaa ggaaatttta tttgctattg atcattttaa
     3721 tcagattgtt cgccatgagc tgctgagaat gatatcatgg aaggtcggca tcgaaacagg
     3781 ctttaaatta agtgtaggca agaactataa gtttattgaa aggtatatat ccgaggattt
     3841 gtgggagaaa cttttgtcca cctaccggat ggattcctat gaaaacatat gggaagcatt
     3901 atttctatgc catcaattgt tcagggcggt atccggtgag gtggcggaaa ggcttcatta
     3961 tgcctatccg gagtatgata ggaatataac aaaatatacc agggacatgt ataaaaaata
     4021 cactggtaaa accggctgcc tggatagcac atatgccgct gatatagaag agaggcggga
     4081 acagtgatta cagaaatgaa agcagggcac ctgaaagata tcgataaacc cagcgaacca
     4141 tttgaggtga taggtaagat tataccgagg tatgaaaacg agaattggac ctttacagaa
     4201 ttactctatg aagcgccata tttaaaaagc taccaagacg aagaggatga agaggatgag
     4261 gaggcagatt gccttgaata tattgacaat actgataaga taatatatct ttactaccaa
     4321 gacgataaat gcgtcggaaa agttaaactg cgaaaaaatt ggaaccggta cgcttatata
     4381 gaagatatcg ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc gcttatcaat
     4441 atatctatag aatgggcaaa gcataaaaac ttgcatggac taatgcttga aacccaggac
     4501 aataacctta tagcttgtaa attctatcat aattgtggtt tcaaaatcgg ctccgtcgat
     4561 actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg
     4621 ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt
     4681 atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac
     4741 cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc
     4801 ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca
     4861 gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg
     4921 aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc
     4981 tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc
     5041 ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat
     5101 cggattgtcc ctatacgaat agcttagaca gccgattagc cgaattggat tacttactga
     5161 ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc
     5221 cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc
     5281 acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg
     5341 atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga
     5401 tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
     5461 agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag
     5521 atttagatgt ctaaaaagct ttaactacaa gctttttaga catctaatct tttctgaagt
     5581 acatccgcaa ctgtccatac tctgatgttt tatatctttt ctaaaagttc gctagataga
     5641 gttctatatt agagatgtaa agagttttgg tgaagaggtt tgtgagtatg gcttttataa
     5701 ccagggtgca atgagaagca ctaacagcag ctagcttaag aacgcatagg atatactttt
     5761 tatggaagtc acagaggagg aataaaattt gaacaggatt aacagagtaa cctctattct
     5821 tattcagctg caatcaaaaa aaataattcc tgccaaagaa attgcacagc gatttaatat
     5881 aagcttaagg gttctgttgc aaagttttaa atctactatc aaataa
//
LOCUS       DACEUF010000223         5852 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.guided.116, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000223 DACEUF010000000
VERSION     DACEUF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.guided.116"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            322..1014
                     /locus_tag="IXG20_002837"
     CDS             322..1014
                     /locus_tag="IXG20_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1610938.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="IXG20_002838"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="IXG20_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1610939.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1910..3862)
                     /locus_tag="IXG20_002839"
     CDS             complement(1910..3862)
                     /locus_tag="IXG20_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ1610940.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3869..4165)
                     /locus_tag="IXG20_002840"
     CDS             complement(3869..4165)
                     /locus_tag="IXG20_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610941.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4507..4797
                     /locus_tag="IXG20_002841"
     CDS             4507..4797
                     /locus_tag="IXG20_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1610942.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            5511..5813
                     /locus_tag="IXG20_002842"
                     /pseudo
     CDS             5511..5813
                     /locus_tag="IXG20_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1998 a          925 c         1010 g         1919 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagcttgaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1861 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1921 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1981 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     2041 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     2101 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2161 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2221 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     2281 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     2341 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     2401 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     2461 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     2521 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     2581 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     2641 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     2701 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     2761 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     2821 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     2881 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     2941 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     3001 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     3061 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     3121 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     3181 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     3241 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     3301 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     3361 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     3421 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     3481 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     3541 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     3601 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     3661 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     3721 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     3781 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     3841 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     3901 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3961 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     4021 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     4081 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     4141 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     4201 tacattttaa cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa
     4261 aacgagtatc aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg
     4321 tactccaaaa ctgaactact taaatctata ctgctgcctc ttttttttag tacatgtcct
     4381 tcatcttcgc aggaagtgcc aatatgattt taagaaaggt aatagacaaa aagtcaaaaa
     4441 gataaaacag agtttcttct ttacaaaggg aatgtatgtt cgtataatgg tattgaggtg
     4501 aaggttatgg agaaaaaata tggaacagta gataagatcc gtactttgca agttagtgcc
     4561 agaccgttga tccgtttcag tttaaatggg gtcaattgtt tgattgcttc ccatagtttg
     4621 aacttcctag ctgaagtaga cgaagggatg aagttagtcg ttacaggtta ttataataat
     4681 agaaagcaat ttgttgtgag atcctatcat gtgttaggga aacccaaaat tgtggtagaa
     4741 tatgagaaaa gtctataccc aagaaaaaag gtccgacaag atgaaagaat aaactagaaa
     4801 acaagcggct gagacaaaaa tgtcctagcc gcttgttttg gaaagatgga tgcagacgat
     4861 gggatttgaa cccacacgag catacgctca caagaacctc aatctatata taactacatg
     4921 tatttaagta tttgtgatga atcactaaac tttccaaact catttgaggg atattgctta
     4981 aagattgaac agatcaattc gaagccctat tagtattcgc tcaatggggg gatcagaggc
     5041 cataaaaaga gactagtcat tactagtctc aggtagtgga attgtaggat tttaaacgtt
     5101 cctggagagt accggtatgt aactcgaaga ggtgattatc atagtcgtaa aagtaaattg
     5161 aagaagcctc gcctttcaca cgagaacgtc ctggttcgac agttaaacct aacatttgga
     5221 tttttgagac aaacgaatct atatcttgtt cgtcaatttt aaaagcaata tgattgtatg
     5281 tctttggcag tttattcgac gaattctgca ttactgcaat ccataggtcc ttaactaaga
     5341 aaaatttttc tggatataat gaaaattttt ttgttgacta tcgtagattt cttttgcatc
     5401 aaataaggta tttaacaatt aagtatttaa aagataactt ttaagttatc tttaagtaat
     5461 gcttaaagct atgttatact ctattaggaa atataagaag gagtataaaa atgaacaaac
     5521 ataatagaaa aataacgaat aacaaagaac tgatcttgtt actaataggg atttggggta
     5581 tctttttact ctttaatatg tatatgccga ctatgagagc ggatgacgta gtttatgcaa
     5641 gtagattaga tgaacttggc tatttgggtg cttcgataga acattataag acatggagtt
     5701 caagaattat tatagaacta ttcttaatgt tcttttctaa acactttatg ctatggaaat
     5761 tattaaattc tacgattatg ttaggaattg tagtattact ttgcaagtat gttgagagta
     5821 taaaatattt tgtgtaaatg aaaaaatcca ta
//
LOCUS       DACEUF010000224         2318 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.guided.154, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000224 DACEUF010000000
VERSION     DACEUF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.guided.154"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            complement(49..1152)
                     /locus_tag="IXG20_002843"
     CDS             complement(49..1152)
                     /locus_tag="IXG20_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1610943.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            1705..2253
                     /gene="aac(6')"
                     /locus_tag="IXG20_002844"
     CDS             1705..2253
                     /gene="aac(6')"
                     /locus_tag="IXG20_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1610944.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          737 a          413 c          449 g          719 t
ORIGIN      
        1 tgattttgca caactcatca gtccttattg acaaagagcc gcaattattt aactgatttt
       61 cttatatcct tgctggcttt cttgtgtagc atgataagtt tctacgtcaa gaatggcctg
      121 tttaaatttt tctcgggtga tttcataagg cacatgttta atatcatttg ttgccatggc
      181 tttatctaag attgcttcta aatcggtcgt gacattgaga ttcatgtcgg ttaacgatac
      241 tggaagttgg ttctttttca taaacacata gattttatca aacaaatcga actgctgatc
      301 catcatcaat aaggtcagta caccataact gacagatgct ccgtgaaggt atccctccat
      361 tttttctaga acagtcgtac cgtaatagaa tgagtgtgcc atagcagaat tatagttatt
      421 gttaactaaa acagaaacca atccggtagt aacaatgata tccgaaacga cttgttcaat
      481 tgcatatgag ggacgatcgt cggcactatc tttcaatgct tgcgtaccat aagtcaagag
      541 acgatcaatg cacatttttc cgatttggat tcctaatcta tccgtgtagc ctagatcatc
      601 gccacgagca gaaaaagtag attcgacttc tttggataaa gcatcgccaa tacctgccca
      661 taagtattta tcaggtgctt cggcaataat tgctgtatca atgaaggtgt gtacaggtgg
      721 ttttgaagga aagaagagtc cgtccattac gccttcttcg ttatagatga cacataccgc
      781 agtgacgggc gaacagttcg aaccaatagt gggaaaggtg aagactggtt tagctagttt
      841 ttcaccaatg acttttacag tatcgcaagc acggccacca ccgactgcaa acaggacgtc
      901 tgctttttgg acactttcat gctggatcaa ctgttcaata tttccataag ttgaattacc
      961 cccgtaccag agactggcga caacctctat ttcctgtgtc aaagctactt ttattttgtt
     1021 ttctgccttt gaaagtgcag tcttccctcc aatcagcact acttttgtcc catattcttt
     1081 gacgattcca tttgcttttt catacgcatc tgttccaatg gtataatttg gtaagaaaac
     1141 actttgactc atattcattt ccttctttct tcgttgaaac gggttaaaat tttaaagagt
     1201 gatcgatctg actgcggctg tagatggagt agaggtgaac ctaagacagg ataagtgatg
     1261 aataaggtgg catgaacacc ttgaaatcat caataagtat cctaaaaacg aacaagtaca
     1321 aaaacattga aaacgacaat aatgaatttt tgttcctctt agtatttcta ccgattccct
     1381 ttgctttttt gtaattatct aaaaattcta aaaattaaga ttattatatg aaaacaggtt
     1441 ttatttgtca attcataatt ggtttttttt aaagcaaaag ataatctata tcatgatgaa
     1501 caaacagagt tttttcctcc aattgatggc tttcctgatc tttcctattt tgaataaagt
     1561 gtttctgtat aattttttta ttttataagt aagataaata atgaaagaat agagatactg
     1621 attggtagct aaagattttt gagcgagaac atttcgtttt ttcaaaagga ctgttagaat
     1681 aagcaagaac aagggggaag acgtatgata atcagtgaat ttgaccgtaa taatccagta
     1741 ttgaaagatc agctttctga tttactgaga ctgacttggc cggaagaata tggagacagc
     1801 tcggcagaag aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc agcggtagac
     1861 caagatgagt tagtaggatt tattggtgca atccctcaat acggtatcac aggttgggaa
     1921 ttgcatccat tagttgtaga aagctcccga cgaaagaacc aaataggtac tcgattagtc
     1981 aattacttag aaaaagaagt agcttccaga ggaggaatca cgatttattt aggtacggat
     2041 gatttagacc atggaacaac gttaagtcaa acggacctgt atgagcatac atttgataaa
     2101 gtggcttcca tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa attaggttat
     2161 aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca aaccggatat ttggatggca
     2221 aaaacgatta ttcctcgacc aaattctcaa taataaaaag aggttgtgac agaagtaatc
     2281 agcttcaaga aataagaagg aatttacgaa aattactt
//
LOCUS       DACEUF010000225         1218 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Flask Transfer
            Population 4 Isolate 8 SAMN10983721-rid9591473.guided.179, whole
            genome shotgun sequence.
ACCESSION   DACEUF010000225 DACEUF010000000
VERSION     DACEUF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983721
            Sequence Read Archive: SRR8602250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705464.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705464.1
            Genome Coverage       :: 861x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:15:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 26 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983721-rid9591473.guided.179"
                     /strain="HOU503"
                     /isolate="Flask Transfer Population 4 Isolate 8"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2018"
                     /collected_by="Amy Prater"
     gene            111..194
                     /locus_tag="IXG20_002845"
     CDS             111..194
                     /locus_tag="IXG20_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1610945.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="IXG20_002846"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="IXG20_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1610946.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          453 a          195 c          199 g          371 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacca ttttgtgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ctgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta cagatgtaat
      301 aacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 attcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggagtat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttttaaatt
     1081 tggaaagtta cacgttacta aagggaatgg agataaatta ttagatatac tactgacagc
     1141 ttccaagaag ctaaagaggt ccctagcgcc tacggggaat ttgggctcta taatttatgg
     1201 gactgatgaa tcagaatt
//
